gRNAs
The following Nup133 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nup133 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OMu41431 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006530964.3 , XM_006530964.4 | |
| Accession Version | XM_006530964.3 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1854bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-06-21 | |
| Organism | Mus musculus(house mouse) | |
| Product | nuclear pore complex protein Nup133 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006530964.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGCTGATG AGCTGTTTTC CTCTCACACG GATTTGGATT CGGACCCTGA GCTAGACAAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006531027.1 |
| CDS | 200..2053 |
| Translation |
Target ORF information:
Target ORF information:
|
 XM_006530964.3 |
1 | ATGGCTGATG AGCTGTTTTC CTCTCACACG GATTTGGATT CGGACCCTGA GCTAGACAAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu41431 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006530964.3 , XM_006530964.4 | |
| Accession Version | XM_006530964.4 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1854bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-08-07 | |
| Organism | Mus musculus(house mouse) | |
| Product | nuclear pore complex protein Nup133 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006530964.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGCTGATG AGCTGTTTTC CTCTCACACG GATTTGGATT CGGACCCTGA GCTAGACAAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006531027.1 |
| CDS | 197..2050 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006530964.4 |
1 | ATGGCTGATG AGCTGTTTTC CTCTCACACG GATTTGGATT CGGACCCTGA GCTAGACAAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu16200 | |
| Clone ID Related Accession (Same CDS sequence) | NM_172288.2 | |
| Accession Version | NM_172288.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3468bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-27 | |
| Organism | Mus musculus(house mouse) | |
| Product | nuclear pore complex protein Nup133 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC123825.5, AK029317.1 and AC147266.6. On Jan 13, 2010 this sequence version replaced NM_172288.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC023915.1, AK029317.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGTTTCCGA GCGTCTCCTC TCCGCGAACC CCAGGGCCGG GGACCCGACG CGGTCCGCTA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_758492.2 |
| CDS | 90..3557 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_172288.2 |
1 | ATGTTTCCGA GCGTCTCCTC TCCGCGAACC CCAGGGCCGG GGACCCGACG CGGTCCGCTA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
A Nup133-dependent NPC-anchored network tethers centrosomes to the nuclear envelope in prophase. |
Nuclear pore composition regulates neural stem/progenitor cell differentiation in the mouse embryo. |
Novel vertebrate nucleoporins Nup133 and Nup160 play a role in mRNA export. |
An evolutionarily conserved NPC subcomplex, which redistributes in part to kinetochores in mammalian cells. |
