Vac14 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vac14
Entrez Gene ID	234729
Full Name	Vac14 homolog (S. cerevisiae)
Synonyms	AA959718,BC032215,D8Wsu151e,Tax1bp2,Trx,ingls
General protein information	Preferred Names Vac14 homolog (S. cerevisiae) Names protein VAC14 homolog Tax1 (human T-cell leukemia virus type I) binding protein 1 Tax1 (human T-cell leukemia virus type I) binding protein 2 vacuole morphology 14
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8 D3-E1\|8 57.59 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006530918.2	XP_006530981.1	protein VAC14 homolog isoform X1
XM_006530918.3	XP_006530981.1	protein VAC14 homolog isoform X1
XM_006530919.3	XP_006530982.1	protein VAC14 homolog isoform X2
NM_146216.2	NP_666328.2	protein VAC14 homolog
NM_146216.3	NP_666328.2	protein VAC14 homolog

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Vac14	NP_808791.1
Canis lupus familiaris (dog)	VAC14	XP_546837.2
Bos taurus (cattle)	VAC14	NP_001075913.1
Danio rerio (zebrafish)	vac14	NP_001004650.1
Kluyveromyces lactis	KLLA0E05193g	XP_002999395.1
Eremothecium gossypii	AGOS_AER049W	NP_984909.2
Neurospora crassa	NCU02409	XP_959537.2
Oryza sativa (rice)	Os03g0223700	NP_001049425.1
Mus musculus (house mouse)	Vac14	NP_666328.2
Drosophila melanogaster (fruit fly)	CG5608	NP_650208.1
Arabidopsis thaliana (thale cress)	AT2G01690	NP_973395.1
Pan troglodytes (chimpanzee)	VAC14	XP_001170940.1
Gallus gallus (chicken)	VAC14	NP_001025735.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000770	XP_311309.5
Caenorhabditis elegans (roundworm)	K04G2.6	NP_492215.1
Schizosaccharomyces pombe (fission yeast)	SPBC25H2.03	NP_596363.1
Magnaporthe oryzae (rice blast fungus)	MGG_03568	XP_003716347.1
Homo sapiens (human)	VAC14	NP_060522.3
	VAC14	XP_001106962.1
Saccharomyces cerevisiae (baker's yeast)	VAC14	NP_013490.3
Xenopus tropicalis (tropical clawed frog)	vac14	NP_001135664.1

Gene Ontology
Bibliography

Related articles in PubMed

A molecular mechanism to regulate lysosome motility for lysosome positioning and tubulation.
Li X, Rydzewski N, Hider A, Zhang X, Yang J, Wang W, Gao Q, Cheng X, Xu H
Nature cell biology18(4)404-17(2016 Apr)

PI(3,5)P2 biosynthesis regulates oligodendrocyte differentiation by intrinsic and extrinsic mechanisms.
Mironova YA, Lenk GM, Lin JP, Lee SJ, Twiss JL, Vaccari I, Bolino A, Havton LA, Min SH, Abrams CS, Shrager P, Meisler MH, Giger RJ
eLife5(2016 03)

Upregulation of Parkin in endophilin mutant mice.
Cao M, Milosevic I, Giovedi S, De Camilli P
The Journal of neuroscience : the official journal of the Society for Neuroscience34(49)16544-9(2014 Dec)

PIKfyve, a class III lipid kinase, is required for TLR-induced type I IFN production via modulation of ATF3.
Cai X, Xu Y, Kim YM, Loureiro J, Huang Q
Journal of immunology (Baltimore, Md. : 1950)192(7)3383-9(2014 Apr)

In vivo, Pikfyve generates PI(3,5)P2, which serves as both a signaling lipid and the major precursor for PI5P.
Zolov SN, Bridges D, Zhang Y, Lee WW, Riehle E, Verma R, Lenk GM, Converso-Baran K, Weide T, Albin RL, Saltiel AR, Meisler MH, Russell MW, Weisman LS
Proceedings of the National Academy of Sciences of the United States of America109(43)17472-7(2012 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Thus, VAC14, PI(3,5)P(2) and/or PI(5)P play a role in controlling postsynaptic function via regulation of endocytic cycling of AMPA receptors.
Title: Modulation of synaptic function by VAC14, a protein that regulates the phosphoinositides PI(3,5)P? and PI(5)P.

a novel regulatory mechanism whereby ArPIKfyve enhances Sac3 abundance by attenuating Sac3 proteasome-dependent degradation and suggest that a failure of this mechanism could be the primary molecular defect in the pathogenesis of CMT4J.
Title: ArPIKfyve regulates Sac3 protein abundance and turnover: disruption of the mechanism by Sac3I41T mutation causing Charcot-Marie-Tooth 4J disorder.

The authors show that Vac14 exerts an effect as a scaffold for the PI(3,5)P2 regulatory complex by direct contact with the known regulators of PI(3,5)P2: Fig4, Fab1, Vac7 and Atg18.
Title: VAC14 nucleates a protein complex essential for the acute interconversion of PI3P and PI(3,5)P(2) in yeast and mouse.

report the characterization of a mouse mutant lacking Vac14, a regulator of PI(3,5)P(2) synthesis
Title: Loss of Vac14, a regulator of the signaling lipid phosphatidylinositol 3,5-bisphosphate, results in neurodegeneration in mice.

The data demonstrate for the first time a physical association between functionally related PIKfyve and ArPIKfyve in 3T3-L1 adipocytes.
Title: ArPIKfyve-PIKfyve interaction and role in insulin-regulated GLUT4 translocation and glucose transport in 3T3-L1 adipocytes.

The following Vac14 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vac14 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu41396	XM_006530919.3 Latest version!	Mus musculus Vac14 homolog (S. cerevisiae) (Vac14), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu12323	NM_146216.3 Latest version!	Mus musculus Vac14 homolog (S. cerevisiae) (Vac14), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OMu41395	XM_006530918.3 Latest version!	Mus musculus Vac14 homolog (S. cerevisiae) (Vac14), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OMu41395	XM_006530918.2	Mus musculus Vac14 homolog (S. cerevisiae) (Vac14), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu12323	NM_146216.2	Mus musculus Vac14 homolog (S. cerevisiae) (Vac14), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vac14 ( XM_006530919.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530982.1 Mus musculus Vac14 homolog (S. cerevisiae) (Vac14), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu41396
Clone ID Related Accession (Same CDS sequence)	XM_006530919.3
Accession Version	XM_006530919.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1710bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein VAC14 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006530919.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCCAG AGAAGGATTT TGCGCCGCTC ACTCCCAACA TCGTAAGGGC CCTGAATGAT 
AAACTCTACG AAAAGCGGAA GGTTGCAGCG CTGGAGATCG AGAAGCTGGT GCGGGACTTT 
GTGGCCCAGA ACAACACCAT GCAAATCAAG CACGTGATCC AGACCCTGTC TCAGGAGTTT 
GCCCTGTCCC AGCACCCCCA CAGCCGGAAA GGGGGCCTCA TCGGTCTGGC AGCCTGCTCT 
ATTGCCCTAG GCAAGGACTC AGGGCTCTAC CTGAAGGAGC TGATAGAGCC AGTGCTGACC 
TGCTTTAATG ACGCAGACAG CAGGCTGCGC TACTACGCTT GTGAGGCCCT CTACAATATC 
GTCAAAGTGG CCCGAGGTGC CGTGCTGCCG CACTTCAATG TTCTCTTCGA TGGACTGAGT 
AAGTTGGCTG CTGACCCAGA CCCCAATGTG AAAAGTGGAT CTGAGCTCCT GGACCGCCTC 
TTAAAGGACA TTGTGACAGA AAGCAGCAAG TTTGACCTGG TGAGCTTCAT CCCCCTTTTG 
CGGGAGAGGA TTTACTCCAA CAACCAATAC GCCCGACAGT TCATCATCTC CTGGATCCTG 
GTTCTGGTGT CGGTGCCAGA CATTAACCTG CTGGATTACC TGCCAGAGAT CCTAGATGGG 
CTCTTCCAGA TCCTGGGTGA CAATGGCAAG GAGATTCGGA AAATGTGTGA GGTGGTTCTT 
GGAGAGTTCT TGAAAGAAAT TAAGAAGAAC CCATCCAGTG TGAAGTTTGC TGAGATGGCC 
AACATCCTGG TGATCCACTG CCAGACCACA GATGACCTGA TTCAGCTGAC GGCTATGTGC 
TGGATGCGGG AATTCATCCA GCTGGCGGGC CGGGTAATGC TGCCCTATTC CTCTGGGATC 
CTGACTGCTG TCCTGCCATG CTTGGCCTAT GATGACCGCA AGAAAAGTAT CAAGGAGGTG 
GCCAATGTGT GTAACCAGAG CTTGATGAAG CTGGTCACCC CTGAGGATGA TGAACCCGAT 
GAGCCAAAGT CAGTAGCACA GAAGCAGACC GAACCCAACC CTGAGGATTC CCTGCCAAAG 
CAGGAGGGGA CAGCCAGTGG AGGTCCAGGT TCCTGTGACT CCAGCTTTGG AAGTGGCATC 
AATGTCTTCA CCTCAGCCAA CACTGATAGG GCTCCAGTGA CCCTGCACCT GGACGGGATT 
GTTCAAGTCC TGAACTGTCA CCTTAGTGAC ACAACCATTG GGATGATGAC CAGGATTGCT 
GTTCTGAAGT GGCTCTACCA TCTCTACATC AAAACTCCTC GAAAGATGTT CCGGCACACA 
GACAGCCTCT TCCCCATCTT ACTTCAGACA TTATCTGACG AATCTGATGA GGTTGTCTTG 
AAGGATCTGG AAGTGTTGGC AGAAATTGCT TCCTCCCCTG CAGGCCAGAC GGATGACCCA 
GGTGCACCTG ATGGCCCTGA CCTCCGAGTC AACCACTCGG AGCTTCAGGT GCCCACCTCC 
GGCAGAGCCA ACCTACTAAA CCCTCCCAGT ACCAAAGGCT TAGAAGGCTC CCCCTCCACT 
CCCACCATGA ACTCCTACTT TTACAAGTTC ATGATCAACC TTCTACAGAC GTTCAGCAGT 
GAACGGAAGC TCCTGGAGGC CAGAGGCCCG TTCATCATCA GGAAAGCCTG TCTATCCTGT 
CCCTGGGGGA AGCCCAAGGA AGAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530982.1
CDS	325..2034
Translation	MNPEKDFAPLTPNIVRALNDKLYEKRKVAALEIEKLVRDFVAQNNTMQIKHVIQTLSQEFALSQHPHSRKGGLIGLAACSIALGKDSGLYLKELIEPVLTCFNDADSRLRYYACEALYNIVKVARGAVLPHFNVLFDGLSKLAADPDPNVKSGSELLDRLLKDIVTESSKFDLVSFIPLLRERIYSNNQYARQFIISWILVLVSVPDINLLDYLPEILDGLFQILGDNGKEIRKMCEVVLGEFLKEIKKNPSSVKFAEMANILVIHCQTTDDLIQLTAMCWMREFIQLAGRVMLPYSSGILTAVLPCLAYDDRKKSIKEVANVCNQSLMKLVTPEDDEPDEPKSVAQKQTEPNPEDSLPKQEGTASGGPGSCDSSFGSGINVFTSANTDRAPVTLHLDGIVQVLNCHLSDTTIGMMTRIAVLKWLYHLYIKTPRKMFRHTDSLFPILLQTLSDESDEVVLKDLEVLAEIASSPAGQTDDPGAPDGPDLRVNHSELQVPTSGRANLLNPPSTKGLEGSPSTPTMNSYFYKFMINLLQTFSSERKLLEARGPFIIRKACLSCPWGKPKEEK

Target ORF information:

RefSeq Version	XM_006530919.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Vac14 homolog (S. cerevisiae) (Vac14), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530919.3

ORF Insert Sequence:

ATGAACCCAG AGAAGGATTT TGCGCCGCTC ACTCCCAACA TCGTAAGGGC CCTGAATGAT 
AAACTCTACG AAAAGCGGAA GGTTGCAGCG CTGGAGATCG AGAAGCTGGT GCGGGACTTT 
GTGGCCCAGA ACAACACCAT GCAAATCAAG CACGTGATCC AGACCCTGTC TCAGGAGTTT 
GCCCTGTCCC AGCACCCCCA CAGCCGGAAA GGGGGCCTCA TCGGTCTGGC AGCCTGCTCT 
ATTGCCCTAG GCAAGGACTC AGGGCTCTAC CTGAAGGAGC TGATAGAGCC AGTGCTGACC 
TGCTTTAATG ACGCAGACAG CAGGCTGCGC TACTACGCTT GTGAGGCCCT CTACAATATC 
GTCAAAGTGG CCCGAGGTGC CGTGCTGCCG CACTTCAATG TTCTCTTCGA TGGACTGAGT 
AAGTTGGCTG CTGACCCAGA CCCCAATGTG AAAAGTGGAT CTGAGCTCCT GGACCGCCTC 
TTAAAGGACA TTGTGACAGA AAGCAGCAAG TTTGACCTGG TGAGCTTCAT CCCCCTTTTG 
CGGGAGAGGA TTTACTCCAA CAACCAATAC GCCCGACAGT TCATCATCTC CTGGATCCTG 
GTTCTGGTGT CGGTGCCAGA CATTAACCTG CTGGATTACC TGCCAGAGAT CCTAGATGGG 
CTCTTCCAGA TCCTGGGTGA CAATGGCAAG GAGATTCGGA AAATGTGTGA GGTGGTTCTT 
GGAGAGTTCT TGAAAGAAAT TAAGAAGAAC CCATCCAGTG TGAAGTTTGC TGAGATGGCC 
AACATCCTGG TGATCCACTG CCAGACCACA GATGACCTGA TTCAGCTGAC GGCTATGTGC 
TGGATGCGGG AATTCATCCA GCTGGCGGGC CGGGTAATGC TGCCCTATTC CTCTGGGATC 
CTGACTGCTG TCCTGCCATG CTTGGCCTAT GATGACCGCA AGAAAAGTAT CAAGGAGGTG 
GCCAATGTGT GTAACCAGAG CTTGATGAAG CTGGTCACCC CTGAGGATGA TGAACCCGAT 
GAGCCAAAGT CAGTAGCACA GAAGCAGACC GAACCCAACC CTGAGGATTC CCTGCCAAAG 
CAGGAGGGGA CAGCCAGTGG AGGTCCAGGT TCCTGTGACT CCAGCTTTGG AAGTGGCATC 
AATGTCTTCA CCTCAGCCAA CACTGATAGG GCTCCAGTGA CCCTGCACCT GGACGGGATT 
GTTCAAGTCC TGAACTGTCA CCTTAGTGAC ACAACCATTG GGATGATGAC CAGGATTGCT 
GTTCTGAAGT GGCTCTACCA TCTCTACATC AAAACTCCTC GAAAGATGTT CCGGCACACA 
GACAGCCTCT TCCCCATCTT ACTTCAGACA TTATCTGACG AATCTGATGA GGTTGTCTTG 
AAGGATCTGG AAGTGTTGGC AGAAATTGCT TCCTCCCCTG CAGGCCAGAC GGATGACCCA 
GGTGCACCTG ATGGCCCTGA CCTCCGAGTC AACCACTCGG AGCTTCAGGT GCCCACCTCC 
GGCAGAGCCA ACCTACTAAA CCCTCCCAGT ACCAAAGGCT TAGAAGGCTC CCCCTCCACT 
CCCACCATGA ACTCCTACTT TTACAAGTTC ATGATCAACC TTCTACAGAC GTTCAGCAGT 
GAACGGAAGC TCCTGGAGGC CAGAGGCCCG TTCATCATCA GGAAAGCCTG TCTATCCTGT 
CCCTGGGGGA AGCCCAAGGA AGAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vac14 ( XM_006530918.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530981.1 Mus musculus Vac14 homolog (S. cerevisiae) (Vac14), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu41395
Clone ID Related Accession (Same CDS sequence)	XM_006530918.2 , XM_006530918.3
Accession Version	XM_006530918.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein VAC14 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006530918.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCCAG AGAAGGATTT TGCGCCGCTC ACTCCCAACA TCGTAAGGGC CCTGAATGAT 
AAACTCTACG AAAAGCGGAA GGTTGCAGCG CTGGAGATCG AGAAGCTGGT GCGGGACTTT 
GTGGCCCAGA ACAACACCAT GCAAATCAAG CACGTGATCC AGACCCTGTC TCAGGAGTTT 
GCCCTGTCCC AGCACCCCCA CAGCCGGAAA GGGGGCCTCA TCGGTCTGGC AGCCTGCTCT 
ATTGCCCTAG GCAAGGACTC AGGGCTCTAC CTGAAGGAGC TGATAGAGCC AGTGCTGACC 
TGCTTTAATG ACGCAGACAG CAGGCTGCGC TACTACGCTT GTGAGGCCCT CTACAATATC 
GTCAAAGTGG CCCGAGGTGC CGTGCTGCCG CACTTCAATG TTCTCTTCGA TGGACTGAGT 
AAGTTGGCTG CTGACCCAGA CCCCAATGTG AAAAGTGGAT CTGAGCTCCT GGACCGCCTC 
TTAAAGGACA TTGTGACAGA AAGCAGCAAG TTTGACCTGG TGAGCTTCAT CCCCCTTTTG 
CGGGAGAGGA TTTACTCCAA CAACCAATAC GCCCGACAGT TCATCATCTC CTGGATCCTG 
GTTCTGGTGT CGGTGCCAGA CATTAACCTG CTGGATTACC TGCCAGAGAT CCTAGATGGG 
CTCTTCCAGA TCCTGGGTGA CAATGGCAAG GAGATTCGGA AAATGTGTGA GGTGGTTCTT 
GGAGAGTTCT TGAAAGAAAT TAAGAAGAAC CCATCCAGTG TGAAGTTTGC TGAGATGGCC 
AACATCCTGG TGATCCACTG CCAGACCACA GATGACCTGA TTCAGCTGAC GGCTATGTGC 
TGGATGCGGG AATTCATCCA GCTGGCGGGC CGGGTAATGC TGCCCTATTC CTCTGGGATC 
CTGACTGCTG TCCTGCCATG CTTGGCCTAT GATGACCGCA AGAAAAGTAT CAAGGAGGTG 
GCCAATGTGT GTAACCAGAG CTTGATGAAG CTGGTCACCC CTGAGGATGA TGAACCCGAT 
GAGCCAAAGT CAGTAGCACA GAAGCAGACC GAACCCAACC CTGAGGATTC CCTGCCAAAG 
CAGGAGGGGA CAGCCAGTGG AGGTCCAGGT TCCTGTGACT CCAGCTTTGG AAGTGGCATC 
AATGTCTTCA CCTCAGCCAA CACTGATAGG GCTCCAGTGA CCCTGCACCT GGACGGGATT 
GTTCAAGTCC TGAACTGTCA CCTTAGTGAC ACAACCATTG GGATGATGAC CAGGATTGCT 
GTTCTGAAGT GGCTCTACCA TCTCTACATC AAAACTCCTC GAAAGATGTT CCGGCACACA 
GACAGCCTCT TCCCCATCTT ACTTCAGACA TTATCTGACG AATCTGATGA GGTTGTCTTG 
AAGGATCTGG AAGTGTTGGC AGAAATTGCT TCCTCCCCTG CAGGCCAGAC GGATGACCCA 
GGTGCACCTG ATGGCCCTGA CCTCCGAGTC AACCACTCGG AGCTTCAGGT GCCCACCTCC 
GGCAGAGCCA ACCTACTAAA CCCTCCCAGT ACCAAAGGCT TAGAAGGCTC CCCCTCCACT 
CCCACCATGA ACTCCTACTT TTACAAGTTC ATGATCAACC TTCTACAGAC GTTCAGCAGT 
GAACGGAAGC TCCTGGAGGC CAGAGGCCCG TTCATCATCA GGCAGCTCTG CCTCCTGCTG 
AATGCAGAGA ACATCTTCCA TTCAATGGCA GACATCCTGC TTCGGGAGGA GGACCTCAAG 
TTCGCGTCCA CCATGGTGCA CACCCTCAAC ACCATCCTGC TCACCTCCAC CGAGCTCTTC 
CAGCTGAGGA ACCAGCTCAA GGACCTGCAG ACCCCGGAAA GCCAGAACCT CTTCTGCTGC 
CTGTACCGCT CCTGGTGCCA CAACCCAGTC ACCACCGTGT CCCTCTGCTT CCTCACCCAG 
AACTACCGCC ACGCCTATGA CCTCATCCAG AAGTTTGGGG ACCTGGAAGT CACAGTGGAT 
TTCCTCACAG AGGTGGACAA ACTCGTGCAG CTAATTGAGT GCCCCATCTT TACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530981.1
CDS	288..2324
Translation	MNPEKDFAPLTPNIVRALNDKLYEKRKVAALEIEKLVRDFVAQNNTMQIKHVIQTLSQEFALSQHPHSRKGGLIGLAACSIALGKDSGLYLKELIEPVLTCFNDADSRLRYYACEALYNIVKVARGAVLPHFNVLFDGLSKLAADPDPNVKSGSELLDRLLKDIVTESSKFDLVSFIPLLRERIYSNNQYARQFIISWILVLVSVPDINLLDYLPEILDGLFQILGDNGKEIRKMCEVVLGEFLKEIKKNPSSVKFAEMANILVIHCQTTDDLIQLTAMCWMREFIQLAGRVMLPYSSGILTAVLPCLAYDDRKKSIKEVANVCNQSLMKLVTPEDDEPDEPKSVAQKQTEPNPEDSLPKQEGTASGGPGSCDSSFGSGINVFTSANTDRAPVTLHLDGIVQVLNCHLSDTTIGMMTRIAVLKWLYHLYIKTPRKMFRHTDSLFPILLQTLSDESDEVVLKDLEVLAEIASSPAGQTDDPGAPDGPDLRVNHSELQVPTSGRANLLNPPSTKGLEGSPSTPTMNSYFYKFMINLLQTFSSERKLLEARGPFIIRQLCLLLNAENIFHSMADILLREEDLKFASTMVHTLNTILLTSTELFQLRNQLKDLQTPESQNLFCCLYRSWCHNPVTTVSLCFLTQNYRHAYDLIQKFGDLEVTVDFLTEVDKLVQLIECPIFT

Target ORF information:

RefSeq Version	XM_006530918.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Vac14 homolog (S. cerevisiae) (Vac14), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530918.2

ORF Insert Sequence:

ATGAACCCAG AGAAGGATTT TGCGCCGCTC ACTCCCAACA TCGTAAGGGC CCTGAATGAT 
AAACTCTACG AAAAGCGGAA GGTTGCAGCG CTGGAGATCG AGAAGCTGGT GCGGGACTTT 
GTGGCCCAGA ACAACACCAT GCAAATCAAG CACGTGATCC AGACCCTGTC TCAGGAGTTT 
GCCCTGTCCC AGCACCCCCA CAGCCGGAAA GGGGGCCTCA TCGGTCTGGC AGCCTGCTCT 
ATTGCCCTAG GCAAGGACTC AGGGCTCTAC CTGAAGGAGC TGATAGAGCC AGTGCTGACC 
TGCTTTAATG ACGCAGACAG CAGGCTGCGC TACTACGCTT GTGAGGCCCT CTACAATATC 
GTCAAAGTGG CCCGAGGTGC CGTGCTGCCG CACTTCAATG TTCTCTTCGA TGGACTGAGT 
AAGTTGGCTG CTGACCCAGA CCCCAATGTG AAAAGTGGAT CTGAGCTCCT GGACCGCCTC 
TTAAAGGACA TTGTGACAGA AAGCAGCAAG TTTGACCTGG TGAGCTTCAT CCCCCTTTTG 
CGGGAGAGGA TTTACTCCAA CAACCAATAC GCCCGACAGT TCATCATCTC CTGGATCCTG 
GTTCTGGTGT CGGTGCCAGA CATTAACCTG CTGGATTACC TGCCAGAGAT CCTAGATGGG 
CTCTTCCAGA TCCTGGGTGA CAATGGCAAG GAGATTCGGA AAATGTGTGA GGTGGTTCTT 
GGAGAGTTCT TGAAAGAAAT TAAGAAGAAC CCATCCAGTG TGAAGTTTGC TGAGATGGCC 
AACATCCTGG TGATCCACTG CCAGACCACA GATGACCTGA TTCAGCTGAC GGCTATGTGC 
TGGATGCGGG AATTCATCCA GCTGGCGGGC CGGGTAATGC TGCCCTATTC CTCTGGGATC 
CTGACTGCTG TCCTGCCATG CTTGGCCTAT GATGACCGCA AGAAAAGTAT CAAGGAGGTG 
GCCAATGTGT GTAACCAGAG CTTGATGAAG CTGGTCACCC CTGAGGATGA TGAACCCGAT 
GAGCCAAAGT CAGTAGCACA GAAGCAGACC GAACCCAACC CTGAGGATTC CCTGCCAAAG 
CAGGAGGGGA CAGCCAGTGG AGGTCCAGGT TCCTGTGACT CCAGCTTTGG AAGTGGCATC 
AATGTCTTCA CCTCAGCCAA CACTGATAGG GCTCCAGTGA CCCTGCACCT GGACGGGATT 
GTTCAAGTCC TGAACTGTCA CCTTAGTGAC ACAACCATTG GGATGATGAC CAGGATTGCT 
GTTCTGAAGT GGCTCTACCA TCTCTACATC AAAACTCCTC GAAAGATGTT CCGGCACACA 
GACAGCCTCT TCCCCATCTT ACTTCAGACA TTATCTGACG AATCTGATGA GGTTGTCTTG 
AAGGATCTGG AAGTGTTGGC AGAAATTGCT TCCTCCCCTG CAGGCCAGAC GGATGACCCA 
GGTGCACCTG ATGGCCCTGA CCTCCGAGTC AACCACTCGG AGCTTCAGGT GCCCACCTCC 
GGCAGAGCCA ACCTACTAAA CCCTCCCAGT ACCAAAGGCT TAGAAGGCTC CCCCTCCACT 
CCCACCATGA ACTCCTACTT TTACAAGTTC ATGATCAACC TTCTACAGAC GTTCAGCAGT 
GAACGGAAGC TCCTGGAGGC CAGAGGCCCG TTCATCATCA GGCAGCTCTG CCTCCTGCTG 
AATGCAGAGA ACATCTTCCA TTCAATGGCA GACATCCTGC TTCGGGAGGA GGACCTCAAG 
TTCGCGTCCA CCATGGTGCA CACCCTCAAC ACCATCCTGC TCACCTCCAC CGAGCTCTTC 
CAGCTGAGGA ACCAGCTCAA GGACCTGCAG ACCCCGGAAA GCCAGAACCT CTTCTGCTGC 
CTGTACCGCT CCTGGTGCCA CAACCCAGTC ACCACCGTGT CCCTCTGCTT CCTCACCCAG 
AACTACCGCC ACGCCTATGA CCTCATCCAG AAGTTTGGGG ACCTGGAAGT CACAGTGGAT 
TTCCTCACAG AGGTGGACAA ACTCGTGCAG CTAATTGAGT GCCCCATCTT TACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vac14 ( NM_146216.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_666328.2 Mus musculus Vac14 homolog (S. cerevisiae) (Vac14), mRNA.

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CloneID	OMu12323
Clone ID Related Accession (Same CDS sequence)	NM_146216.2 , NM_146216.3
Accession Version	NM_146216.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2349bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-24
Organism	Mus musculus(House mouse)
Product	protein VAC14 homolog
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC052199.1. On Jun 9, 2003 this sequence version replaced NM_146216.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC052199.1, AK046826.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END##

ATGAACCCAG AGAAGGATTT TGCGCCGCTC ACTCCCAACA TCGTAAGGGC CCTGAATGAT 
AAACTCTACG AAAAGCGGAA GGTTGCAGCG CTGGAGATCG AGAAGCTGGT GCGGGACTTT 
GTGGCCCAGA ACAACACCAT GCAAATCAAG CACGTGATCC AGACCCTGTC TCAGGAGTTT 
GCCCTGTCCC AGCACCCCCA CAGCCGGAAA GGGGGCCTCA TCGGTCTGGC AGCCTGCTCT 
ATTGCCCTAG GCAAGGACTC AGGGCTCTAC CTGAAGGAGC TGATAGAGCC AGTGCTGACC 
TGCTTTAATG ACGCAGACAG CAGGCTGCGC TACTACGCTT GTGAGGCCCT CTACAATATC 
GTCAAAGTGG CCCGAGGTGC CGTGCTGCCG CACTTCAATG TTCTCTTCGA TGGACTGAGT 
AAGTTGGCTG CTGACCCAGA CCCCAATGTG AAAAGTGGAT CTGAGCTCCT GGACCGCCTC 
TTAAAGGACA TTGTGACAGA AAGCAGCAAG TTTGACCTGG TGAGCTTCAT CCCCCTTTTG 
CGGGAGAGGA TTTACTCCAA CAACCAATAC GCCCGACAGT TCATCATCTC CTGGATCCTG 
GTTCTGGTGT CGGTGCCAGA CATTAACCTG CTGGATTACC TGCCAGAGAT CCTAGATGGG 
CTCTTCCAGA TCCTGGGTGA CAATGGCAAG GAGATTCGGA AAATGTGTGA GGTGGTTCTT 
GGAGAGTTCT TGAAAGAAAT TAAGAAGAAC CCATCCAGTG TGAAGTTTGC TGAGATGGCC 
AACATCCTGG TGATCCACTG CCAGACCACA GATGACCTGA TTCAGCTGAC GGCTATGTGC 
TGGATGCGGG AATTCATCCA GCTGGCGGGC CGGGTAATGC TGCCCTATTC CTCTGGGATC 
CTGACTGCTG TCCTGCCATG CTTGGCCTAT GATGACCGCA AGAAAAGTAT CAAGGAGGTG 
GCCAATGTGT GTAACCAGAG CTTGATGAAG CTGGTCACCC CTGAGGATGA TGAACCCGAT 
GAGCCAAAGT CAGTAGCACA GAAGCAGACC GAACCCAACC CTGAGGATTC CCTGCCAAAG 
CAGGAGGGGA CAGCCAGTGG AGGTCCAGGT TCCTGTGACT CCAGCTTTGG AAGTGGCATC 
AATGTCTTCA CCTCAGCCAA CACTGATAGG GCTCCAGTGA CCCTGCACCT GGACGGGATT 
GTTCAAGTCC TGAACTGTCA CCTTAGTGAC ACAACCATTG GGATGATGAC CAGGATTGCT 
GTTCTGAAGT GGCTCTACCA TCTCTACATC AAAACTCCTC GAAAGATGTT CCGGCACACA 
GACAGCCTCT TCCCCATCTT ACTTCAGACA TTATCTGACG AATCTGATGA GGTTGTCTTG 
AAGGATCTGG AAGTGTTGGC AGAAATTGCT TCCTCCCCTG CAGGCCAGAC GGATGACCCA 
GGTGCACCTG ATGGCCCTGA CCTCCGAGTC AACCACTCGG AGCTTCAGGT GCCCACCTCC 
GGCAGAGCCA ACCTACTAAA CCCTCCCAGT ACCAAAGGCT TAGAAGGCTC CCCCTCCACT 
CCCACCATGA ACTCCTACTT TTACAAGTTC ATGATCAACC TTCTACAGAC GTTCAGCAGT 
GAACGGAAGC TCCTGGAGGC CAGAGGCCCG TTCATCATCA GGCAGCTCTG CCTCCTGCTG 
AATGCAGAGA ACATCTTCCA TTCAATGGCA GACATCCTGC TTCGGGAGGA GGACCTCAAG 
TTCGCGTCCA CCATGGTGCA CACCCTCAAC ACCATCCTGC TCACCTCCAC CGAGCTCTTC 
CAGCTGAGGA ACCAGCTCAA GGACCTGCAG ACCCCGGAAA GCCAGAACCT CTTCTGCTGC 
CTGTACCGCT CCTGGTGCCA CAACCCAGTC ACCACCGTGT CCCTCTGCTT CCTCACCCAG 
AACTACCGCC ACGCCTATGA CCTCATCCAG AAGTTTGGGG ACCTGGAAGT CACAGTGGAT 
TTCCTCACAG AGGTGGACAA ACTCGTGCAG CTAATTGAGT GCCCCATCTT TACATATCTA 
CGCCTGCAAC TGCTGGACGT GAAGAACAAC CCATACCTGA TCAAGGCCCT GTACGGCCTG 
CTCATGCTGT TGCCCCAGAG CAGTGCCTTC CAGCTGCTCT CTCACCGGCT CCAGTGTGTG 
CCCAACCCTG AGCTGCTGCA GACTGAAGAC TGTCTGAAAG CGGCCCCCAA GTCCCAGAAA 
GGTGACTCCC CCAGCATCGA CTACACAGAG CTGCTGCAGC ACTTTGAAAA AGTCCAGAAA 
CAGCACCTGG AAGTAAGACA CCAGCGCAGT GGGCGAGGGG ATCACCTGGA CCGCAGAGTT 
ATCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_666328.2
CDS	253..2601
Translation	MNPEKDFAPLTPNIVRALNDKLYEKRKVAALEIEKLVRDFVAQNNTMQIKHVIQTLSQEFALSQHPHSRKGGLIGLAACSIALGKDSGLYLKELIEPVLTCFNDADSRLRYYACEALYNIVKVARGAVLPHFNVLFDGLSKLAADPDPNVKSGSELLDRLLKDIVTESSKFDLVSFIPLLRERIYSNNQYARQFIISWILVLVSVPDINLLDYLPEILDGLFQILGDNGKEIRKMCEVVLGEFLKEIKKNPSSVKFAEMANILVIHCQTTDDLIQLTAMCWMREFIQLAGRVMLPYSSGILTAVLPCLAYDDRKKSIKEVANVCNQSLMKLVTPEDDEPDEPKSVAQKQTEPNPEDSLPKQEGTASGGPGSCDSSFGSGINVFTSANTDRAPVTLHLDGIVQVLNCHLSDTTIGMMTRIAVLKWLYHLYIKTPRKMFRHTDSLFPILLQTLSDESDEVVLKDLEVLAEIASSPAGQTDDPGAPDGPDLRVNHSELQVPTSGRANLLNPPSTKGLEGSPSTPTMNSYFYKFMINLLQTFSSERKLLEARGPFIIRQLCLLLNAENIFHSMADILLREEDLKFASTMVHTLNTILLTSTELFQLRNQLKDLQTPESQNLFCCLYRSWCHNPVTTVSLCFLTQNYRHAYDLIQKFGDLEVTVDFLTEVDKLVQLIECPIFTYLRLQLLDVKNNPYLIKALYGLLMLLPQSSAFQLLSHRLQCVPNPELLQTEDCLKAAPKSQKGDSPSIDYTELLQHFEKVQKQHLEVRHQRSGRGDHLDRRVIL

Target ORF information:

RefSeq Version	NM_146216.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus Vac14 homolog (S. cerevisiae) (Vac14), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_146216.2

ORF Insert Sequence:

ATGAACCCAG AGAAGGATTT TGCGCCGCTC ACTCCCAACA TCGTAAGGGC CCTGAATGAT 
AAACTCTACG AAAAGCGGAA GGTTGCAGCG CTGGAGATCG AGAAGCTGGT GCGGGACTTT 
GTGGCCCAGA ACAACACCAT GCAAATCAAG CACGTGATCC AGACCCTGTC TCAGGAGTTT 
GCCCTGTCCC AGCACCCCCA CAGCCGGAAA GGGGGCCTCA TCGGTCTGGC AGCCTGCTCT 
ATTGCCCTAG GCAAGGACTC AGGGCTCTAC CTGAAGGAGC TGATAGAGCC AGTGCTGACC 
TGCTTTAATG ACGCAGACAG CAGGCTGCGC TACTACGCTT GTGAGGCCCT CTACAATATC 
GTCAAAGTGG CCCGAGGTGC CGTGCTGCCG CACTTCAATG TTCTCTTCGA TGGACTGAGT 
AAGTTGGCTG CTGACCCAGA CCCCAATGTG AAAAGTGGAT CTGAGCTCCT GGACCGCCTC 
TTAAAGGACA TTGTGACAGA AAGCAGCAAG TTTGACCTGG TGAGCTTCAT CCCCCTTTTG 
CGGGAGAGGA TTTACTCCAA CAACCAATAC GCCCGACAGT TCATCATCTC CTGGATCCTG 
GTTCTGGTGT CGGTGCCAGA CATTAACCTG CTGGATTACC TGCCAGAGAT CCTAGATGGG 
CTCTTCCAGA TCCTGGGTGA CAATGGCAAG GAGATTCGGA AAATGTGTGA GGTGGTTCTT 
GGAGAGTTCT TGAAAGAAAT TAAGAAGAAC CCATCCAGTG TGAAGTTTGC TGAGATGGCC 
AACATCCTGG TGATCCACTG CCAGACCACA GATGACCTGA TTCAGCTGAC GGCTATGTGC 
TGGATGCGGG AATTCATCCA GCTGGCGGGC CGGGTAATGC TGCCCTATTC CTCTGGGATC 
CTGACTGCTG TCCTGCCATG CTTGGCCTAT GATGACCGCA AGAAAAGTAT CAAGGAGGTG 
GCCAATGTGT GTAACCAGAG CTTGATGAAG CTGGTCACCC CTGAGGATGA TGAACCCGAT 
GAGCCAAAGT CAGTAGCACA GAAGCAGACC GAACCCAACC CTGAGGATTC CCTGCCAAAG 
CAGGAGGGGA CAGCCAGTGG AGGTCCAGGT TCCTGTGACT CCAGCTTTGG AAGTGGCATC 
AATGTCTTCA CCTCAGCCAA CACTGATAGG GCTCCAGTGA CCCTGCACCT GGACGGGATT 
GTTCAAGTCC TGAACTGTCA CCTTAGTGAC ACAACCATTG GGATGATGAC CAGGATTGCT 
GTTCTGAAGT GGCTCTACCA TCTCTACATC AAAACTCCTC GAAAGATGTT CCGGCACACA 
GACAGCCTCT TCCCCATCTT ACTTCAGACA TTATCTGACG AATCTGATGA GGTTGTCTTG 
AAGGATCTGG AAGTGTTGGC AGAAATTGCT TCCTCCCCTG CAGGCCAGAC GGATGACCCA 
GGTGCACCTG ATGGCCCTGA CCTCCGAGTC AACCACTCGG AGCTTCAGGT GCCCACCTCC 
GGCAGAGCCA ACCTACTAAA CCCTCCCAGT ACCAAAGGCT TAGAAGGCTC CCCCTCCACT 
CCCACCATGA ACTCCTACTT TTACAAGTTC ATGATCAACC TTCTACAGAC GTTCAGCAGT 
GAACGGAAGC TCCTGGAGGC CAGAGGCCCG TTCATCATCA GGCAGCTCTG CCTCCTGCTG 
AATGCAGAGA ACATCTTCCA TTCAATGGCA GACATCCTGC TTCGGGAGGA GGACCTCAAG 
TTCGCGTCCA CCATGGTGCA CACCCTCAAC ACCATCCTGC TCACCTCCAC CGAGCTCTTC 
CAGCTGAGGA ACCAGCTCAA GGACCTGCAG ACCCCGGAAA GCCAGAACCT CTTCTGCTGC 
CTGTACCGCT CCTGGTGCCA CAACCCAGTC ACCACCGTGT CCCTCTGCTT CCTCACCCAG 
AACTACCGCC ACGCCTATGA CCTCATCCAG AAGTTTGGGG ACCTGGAAGT CACAGTGGAT 
TTCCTCACAG AGGTGGACAA ACTCGTGCAG CTAATTGAGT GCCCCATCTT TACATATCTA 
CGCCTGCAAC TGCTGGACGT GAAGAACAAC CCATACCTGA TCAAGGCCCT GTACGGCCTG 
CTCATGCTGT TGCCCCAGAG CAGTGCCTTC CAGCTGCTCT CTCACCGGCT CCAGTGTGTG 
CCCAACCCTG AGCTGCTGCA GACTGAAGAC TGTCTGAAAG CGGCCCCCAA GTCCCAGAAA 
GGTGACTCCC CCAGCATCGA CTACACAGAG CTGCTGCAGC ACTTTGAAAA AGTCCAGAAA 
CAGCACCTGG AAGTAAGACA CCAGCGCAGT GGGCGAGGGG ATCACCTGGA CCGCAGAGTT 
ATCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vac14 ( NM_146216.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_666328.2 Mus musculus Vac14 homolog (S. cerevisiae) (Vac14), transcript variant 1, mRNA.

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CloneID	OMu12323
Clone ID Related Accession (Same CDS sequence)	NM_146216.2 , NM_146216.3
Accession Version	NM_146216.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2349bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Mus musculus(house mouse)
Product	protein VAC14 homolog
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC069308.21 and BC052199.1. On Oct 26, 2017 this sequence version replaced NM_146216.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC052199.1, AK046826.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACCCAG AGAAGGATTT TGCGCCGCTC ACTCCCAACA TCGTAAGGGC CCTGAATGAT 
AAACTCTACG AAAAGCGGAA GGTTGCAGCG CTGGAGATCG AGAAGCTGGT GCGGGACTTT 
GTGGCCCAGA ACAACACCAT GCAAATCAAG CACGTGATCC AGACCCTGTC TCAGGAGTTT 
GCCCTGTCCC AGCACCCCCA CAGCCGGAAA GGGGGCCTCA TCGGTCTGGC AGCCTGCTCT 
ATTGCCCTAG GCAAGGACTC AGGGCTCTAC CTGAAGGAGC TGATAGAGCC AGTGCTGACC 
TGCTTTAATG ACGCAGACAG CAGGCTGCGC TACTACGCTT GTGAGGCCCT CTACAATATC 
GTCAAAGTGG CCCGAGGTGC CGTGCTGCCG CACTTCAATG TTCTCTTCGA TGGACTGAGT 
AAGTTGGCTG CTGACCCAGA CCCCAATGTG AAAAGTGGAT CTGAGCTCCT GGACCGCCTC 
TTAAAGGACA TTGTGACAGA AAGCAGCAAG TTTGACCTGG TGAGCTTCAT CCCCCTTTTG 
CGGGAGAGGA TTTACTCCAA CAACCAATAC GCCCGACAGT TCATCATCTC CTGGATCCTG 
GTTCTGGTGT CGGTGCCAGA CATTAACCTG CTGGATTACC TGCCAGAGAT CCTAGATGGG 
CTCTTCCAGA TCCTGGGTGA CAATGGCAAG GAGATTCGGA AAATGTGTGA GGTGGTTCTT 
GGAGAGTTCT TGAAAGAAAT TAAGAAGAAC CCATCCAGTG TGAAGTTTGC TGAGATGGCC 
AACATCCTGG TGATCCACTG CCAGACCACA GATGACCTGA TTCAGCTGAC GGCTATGTGC 
TGGATGCGGG AATTCATCCA GCTGGCGGGC CGGGTAATGC TGCCCTATTC CTCTGGGATC 
CTGACTGCTG TCCTGCCATG CTTGGCCTAT GATGACCGCA AGAAAAGTAT CAAGGAGGTG 
GCCAATGTGT GTAACCAGAG CTTGATGAAG CTGGTCACCC CTGAGGATGA TGAACCCGAT 
GAGCCAAAGT CAGTAGCACA GAAGCAGACC GAACCCAACC CTGAGGATTC CCTGCCAAAG 
CAGGAGGGGA CAGCCAGTGG AGGTCCAGGT TCCTGTGACT CCAGCTTTGG AAGTGGCATC 
AATGTCTTCA CCTCAGCCAA CACTGATAGG GCTCCAGTGA CCCTGCACCT GGACGGGATT 
GTTCAAGTCC TGAACTGTCA CCTTAGTGAC ACAACCATTG GGATGATGAC CAGGATTGCT 
GTTCTGAAGT GGCTCTACCA TCTCTACATC AAAACTCCTC GAAAGATGTT CCGGCACACA 
GACAGCCTCT TCCCCATCTT ACTTCAGACA TTATCTGACG AATCTGATGA GGTTGTCTTG 
AAGGATCTGG AAGTGTTGGC AGAAATTGCT TCCTCCCCTG CAGGCCAGAC GGATGACCCA 
GGTGCACCTG ATGGCCCTGA CCTCCGAGTC AACCACTCGG AGCTTCAGGT GCCCACCTCC 
GGCAGAGCCA ACCTACTAAA CCCTCCCAGT ACCAAAGGCT TAGAAGGCTC CCCCTCCACT 
CCCACCATGA ACTCCTACTT TTACAAGTTC ATGATCAACC TTCTACAGAC GTTCAGCAGT 
GAACGGAAGC TCCTGGAGGC CAGAGGCCCG TTCATCATCA GGCAGCTCTG CCTCCTGCTG 
AATGCAGAGA ACATCTTCCA TTCAATGGCA GACATCCTGC TTCGGGAGGA GGACCTCAAG 
TTCGCGTCCA CCATGGTGCA CACCCTCAAC ACCATCCTGC TCACCTCCAC CGAGCTCTTC 
CAGCTGAGGA ACCAGCTCAA GGACCTGCAG ACCCCGGAAA GCCAGAACCT CTTCTGCTGC 
CTGTACCGCT CCTGGTGCCA CAACCCAGTC ACCACCGTGT CCCTCTGCTT CCTCACCCAG 
AACTACCGCC ACGCCTATGA CCTCATCCAG AAGTTTGGGG ACCTGGAAGT CACAGTGGAT 
TTCCTCACAG AGGTGGACAA ACTCGTGCAG CTAATTGAGT GCCCCATCTT TACATATCTA 
CGCCTGCAAC TGCTGGACGT GAAGAACAAC CCATACCTGA TCAAGGCCCT GTACGGCCTG 
CTCATGCTGT TGCCCCAGAG CAGTGCCTTC CAGCTGCTCT CTCACCGGCT CCAGTGTGTG 
CCCAACCCTG AGCTGCTGCA GACTGAAGAC TGTCTGAAAG CGGCCCCCAA GTCCCAGAAA 
GGTGACTCCC CCAGCATCGA CTACACAGAG CTGCTGCAGC ACTTTGAAAA AGTCCAGAAA 
CAGCACCTGG AAGTAAGACA CCAGCGCAGT GGGCGAGGGG ATCACCTGGA CCGCAGAGTT 
ATCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_666328.2
CDS	324..2672
Translation	MNPEKDFAPLTPNIVRALNDKLYEKRKVAALEIEKLVRDFVAQNNTMQIKHVIQTLSQEFALSQHPHSRKGGLIGLAACSIALGKDSGLYLKELIEPVLTCFNDADSRLRYYACEALYNIVKVARGAVLPHFNVLFDGLSKLAADPDPNVKSGSELLDRLLKDIVTESSKFDLVSFIPLLRERIYSNNQYARQFIISWILVLVSVPDINLLDYLPEILDGLFQILGDNGKEIRKMCEVVLGEFLKEIKKNPSSVKFAEMANILVIHCQTTDDLIQLTAMCWMREFIQLAGRVMLPYSSGILTAVLPCLAYDDRKKSIKEVANVCNQSLMKLVTPEDDEPDEPKSVAQKQTEPNPEDSLPKQEGTASGGPGSCDSSFGSGINVFTSANTDRAPVTLHLDGIVQVLNCHLSDTTIGMMTRIAVLKWLYHLYIKTPRKMFRHTDSLFPILLQTLSDESDEVVLKDLEVLAEIASSPAGQTDDPGAPDGPDLRVNHSELQVPTSGRANLLNPPSTKGLEGSPSTPTMNSYFYKFMINLLQTFSSERKLLEARGPFIIRQLCLLLNAENIFHSMADILLREEDLKFASTMVHTLNTILLTSTELFQLRNQLKDLQTPESQNLFCCLYRSWCHNPVTTVSLCFLTQNYRHAYDLIQKFGDLEVTVDFLTEVDKLVQLIECPIFTYLRLQLLDVKNNPYLIKALYGLLMLLPQSSAFQLLSHRLQCVPNPELLQTEDCLKAAPKSQKGDSPSIDYTELLQHFEKVQKQHLEVRHQRSGRGDHLDRRVIL

Target ORF information:

RefSeq Version	NM_146216.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Vac14 homolog (S. cerevisiae) (Vac14), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_146216.3

ORF Insert Sequence:

ATGAACCCAG AGAAGGATTT TGCGCCGCTC ACTCCCAACA TCGTAAGGGC CCTGAATGAT 
AAACTCTACG AAAAGCGGAA GGTTGCAGCG CTGGAGATCG AGAAGCTGGT GCGGGACTTT 
GTGGCCCAGA ACAACACCAT GCAAATCAAG CACGTGATCC AGACCCTGTC TCAGGAGTTT 
GCCCTGTCCC AGCACCCCCA CAGCCGGAAA GGGGGCCTCA TCGGTCTGGC AGCCTGCTCT 
ATTGCCCTAG GCAAGGACTC AGGGCTCTAC CTGAAGGAGC TGATAGAGCC AGTGCTGACC 
TGCTTTAATG ACGCAGACAG CAGGCTGCGC TACTACGCTT GTGAGGCCCT CTACAATATC 
GTCAAAGTGG CCCGAGGTGC CGTGCTGCCG CACTTCAATG TTCTCTTCGA TGGACTGAGT 
AAGTTGGCTG CTGACCCAGA CCCCAATGTG AAAAGTGGAT CTGAGCTCCT GGACCGCCTC 
TTAAAGGACA TTGTGACAGA AAGCAGCAAG TTTGACCTGG TGAGCTTCAT CCCCCTTTTG 
CGGGAGAGGA TTTACTCCAA CAACCAATAC GCCCGACAGT TCATCATCTC CTGGATCCTG 
GTTCTGGTGT CGGTGCCAGA CATTAACCTG CTGGATTACC TGCCAGAGAT CCTAGATGGG 
CTCTTCCAGA TCCTGGGTGA CAATGGCAAG GAGATTCGGA AAATGTGTGA GGTGGTTCTT 
GGAGAGTTCT TGAAAGAAAT TAAGAAGAAC CCATCCAGTG TGAAGTTTGC TGAGATGGCC 
AACATCCTGG TGATCCACTG CCAGACCACA GATGACCTGA TTCAGCTGAC GGCTATGTGC 
TGGATGCGGG AATTCATCCA GCTGGCGGGC CGGGTAATGC TGCCCTATTC CTCTGGGATC 
CTGACTGCTG TCCTGCCATG CTTGGCCTAT GATGACCGCA AGAAAAGTAT CAAGGAGGTG 
GCCAATGTGT GTAACCAGAG CTTGATGAAG CTGGTCACCC CTGAGGATGA TGAACCCGAT 
GAGCCAAAGT CAGTAGCACA GAAGCAGACC GAACCCAACC CTGAGGATTC CCTGCCAAAG 
CAGGAGGGGA CAGCCAGTGG AGGTCCAGGT TCCTGTGACT CCAGCTTTGG AAGTGGCATC 
AATGTCTTCA CCTCAGCCAA CACTGATAGG GCTCCAGTGA CCCTGCACCT GGACGGGATT 
GTTCAAGTCC TGAACTGTCA CCTTAGTGAC ACAACCATTG GGATGATGAC CAGGATTGCT 
GTTCTGAAGT GGCTCTACCA TCTCTACATC AAAACTCCTC GAAAGATGTT CCGGCACACA 
GACAGCCTCT TCCCCATCTT ACTTCAGACA TTATCTGACG AATCTGATGA GGTTGTCTTG 
AAGGATCTGG AAGTGTTGGC AGAAATTGCT TCCTCCCCTG CAGGCCAGAC GGATGACCCA 
GGTGCACCTG ATGGCCCTGA CCTCCGAGTC AACCACTCGG AGCTTCAGGT GCCCACCTCC 
GGCAGAGCCA ACCTACTAAA CCCTCCCAGT ACCAAAGGCT TAGAAGGCTC CCCCTCCACT 
CCCACCATGA ACTCCTACTT TTACAAGTTC ATGATCAACC TTCTACAGAC GTTCAGCAGT 
GAACGGAAGC TCCTGGAGGC CAGAGGCCCG TTCATCATCA GGCAGCTCTG CCTCCTGCTG 
AATGCAGAGA ACATCTTCCA TTCAATGGCA GACATCCTGC TTCGGGAGGA GGACCTCAAG 
TTCGCGTCCA CCATGGTGCA CACCCTCAAC ACCATCCTGC TCACCTCCAC CGAGCTCTTC 
CAGCTGAGGA ACCAGCTCAA GGACCTGCAG ACCCCGGAAA GCCAGAACCT CTTCTGCTGC 
CTGTACCGCT CCTGGTGCCA CAACCCAGTC ACCACCGTGT CCCTCTGCTT CCTCACCCAG 
AACTACCGCC ACGCCTATGA CCTCATCCAG AAGTTTGGGG ACCTGGAAGT CACAGTGGAT 
TTCCTCACAG AGGTGGACAA ACTCGTGCAG CTAATTGAGT GCCCCATCTT TACATATCTA 
CGCCTGCAAC TGCTGGACGT GAAGAACAAC CCATACCTGA TCAAGGCCCT GTACGGCCTG 
CTCATGCTGT TGCCCCAGAG CAGTGCCTTC CAGCTGCTCT CTCACCGGCT CCAGTGTGTG 
CCCAACCCTG AGCTGCTGCA GACTGAAGAC TGTCTGAAAG CGGCCCCCAA GTCCCAGAAA 
GGTGACTCCC CCAGCATCGA CTACACAGAG CTGCTGCAGC ACTTTGAAAA AGTCCAGAAA 
CAGCACCTGG AAGTAAGACA CCAGCGCAGT GGGCGAGGGG ATCACCTGGA CCGCAGAGTT 
ATCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vac14 ( XM_006530918.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006530981.1 Mus musculus Vac14 homolog (S. cerevisiae) (Vac14), transcript variant X2, mRNA.

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CloneID	OMu41395
Clone ID Related Accession (Same CDS sequence)	XM_006530918.2 , XM_006530918.3
Accession Version	XM_006530918.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein VAC14 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006530918.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCCAG AGAAGGATTT TGCGCCGCTC ACTCCCAACA TCGTAAGGGC CCTGAATGAT 
AAACTCTACG AAAAGCGGAA GGTTGCAGCG CTGGAGATCG AGAAGCTGGT GCGGGACTTT 
GTGGCCCAGA ACAACACCAT GCAAATCAAG CACGTGATCC AGACCCTGTC TCAGGAGTTT 
GCCCTGTCCC AGCACCCCCA CAGCCGGAAA GGGGGCCTCA TCGGTCTGGC AGCCTGCTCT 
ATTGCCCTAG GCAAGGACTC AGGGCTCTAC CTGAAGGAGC TGATAGAGCC AGTGCTGACC 
TGCTTTAATG ACGCAGACAG CAGGCTGCGC TACTACGCTT GTGAGGCCCT CTACAATATC 
GTCAAAGTGG CCCGAGGTGC CGTGCTGCCG CACTTCAATG TTCTCTTCGA TGGACTGAGT 
AAGTTGGCTG CTGACCCAGA CCCCAATGTG AAAAGTGGAT CTGAGCTCCT GGACCGCCTC 
TTAAAGGACA TTGTGACAGA AAGCAGCAAG TTTGACCTGG TGAGCTTCAT CCCCCTTTTG 
CGGGAGAGGA TTTACTCCAA CAACCAATAC GCCCGACAGT TCATCATCTC CTGGATCCTG 
GTTCTGGTGT CGGTGCCAGA CATTAACCTG CTGGATTACC TGCCAGAGAT CCTAGATGGG 
CTCTTCCAGA TCCTGGGTGA CAATGGCAAG GAGATTCGGA AAATGTGTGA GGTGGTTCTT 
GGAGAGTTCT TGAAAGAAAT TAAGAAGAAC CCATCCAGTG TGAAGTTTGC TGAGATGGCC 
AACATCCTGG TGATCCACTG CCAGACCACA GATGACCTGA TTCAGCTGAC GGCTATGTGC 
TGGATGCGGG AATTCATCCA GCTGGCGGGC CGGGTAATGC TGCCCTATTC CTCTGGGATC 
CTGACTGCTG TCCTGCCATG CTTGGCCTAT GATGACCGCA AGAAAAGTAT CAAGGAGGTG 
GCCAATGTGT GTAACCAGAG CTTGATGAAG CTGGTCACCC CTGAGGATGA TGAACCCGAT 
GAGCCAAAGT CAGTAGCACA GAAGCAGACC GAACCCAACC CTGAGGATTC CCTGCCAAAG 
CAGGAGGGGA CAGCCAGTGG AGGTCCAGGT TCCTGTGACT CCAGCTTTGG AAGTGGCATC 
AATGTCTTCA CCTCAGCCAA CACTGATAGG GCTCCAGTGA CCCTGCACCT GGACGGGATT 
GTTCAAGTCC TGAACTGTCA CCTTAGTGAC ACAACCATTG GGATGATGAC CAGGATTGCT 
GTTCTGAAGT GGCTCTACCA TCTCTACATC AAAACTCCTC GAAAGATGTT CCGGCACACA 
GACAGCCTCT TCCCCATCTT ACTTCAGACA TTATCTGACG AATCTGATGA GGTTGTCTTG 
AAGGATCTGG AAGTGTTGGC AGAAATTGCT TCCTCCCCTG CAGGCCAGAC GGATGACCCA 
GGTGCACCTG ATGGCCCTGA CCTCCGAGTC AACCACTCGG AGCTTCAGGT GCCCACCTCC 
GGCAGAGCCA ACCTACTAAA CCCTCCCAGT ACCAAAGGCT TAGAAGGCTC CCCCTCCACT 
CCCACCATGA ACTCCTACTT TTACAAGTTC ATGATCAACC TTCTACAGAC GTTCAGCAGT 
GAACGGAAGC TCCTGGAGGC CAGAGGCCCG TTCATCATCA GGCAGCTCTG CCTCCTGCTG 
AATGCAGAGA ACATCTTCCA TTCAATGGCA GACATCCTGC TTCGGGAGGA GGACCTCAAG 
TTCGCGTCCA CCATGGTGCA CACCCTCAAC ACCATCCTGC TCACCTCCAC CGAGCTCTTC 
CAGCTGAGGA ACCAGCTCAA GGACCTGCAG ACCCCGGAAA GCCAGAACCT CTTCTGCTGC 
CTGTACCGCT CCTGGTGCCA CAACCCAGTC ACCACCGTGT CCCTCTGCTT CCTCACCCAG 
AACTACCGCC ACGCCTATGA CCTCATCCAG AAGTTTGGGG ACCTGGAAGT CACAGTGGAT 
TTCCTCACAG AGGTGGACAA ACTCGTGCAG CTAATTGAGT GCCCCATCTT TACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006530981.1
CDS	362..2398
Translation	MNPEKDFAPLTPNIVRALNDKLYEKRKVAALEIEKLVRDFVAQNNTMQIKHVIQTLSQEFALSQHPHSRKGGLIGLAACSIALGKDSGLYLKELIEPVLTCFNDADSRLRYYACEALYNIVKVARGAVLPHFNVLFDGLSKLAADPDPNVKSGSELLDRLLKDIVTESSKFDLVSFIPLLRERIYSNNQYARQFIISWILVLVSVPDINLLDYLPEILDGLFQILGDNGKEIRKMCEVVLGEFLKEIKKNPSSVKFAEMANILVIHCQTTDDLIQLTAMCWMREFIQLAGRVMLPYSSGILTAVLPCLAYDDRKKSIKEVANVCNQSLMKLVTPEDDEPDEPKSVAQKQTEPNPEDSLPKQEGTASGGPGSCDSSFGSGINVFTSANTDRAPVTLHLDGIVQVLNCHLSDTTIGMMTRIAVLKWLYHLYIKTPRKMFRHTDSLFPILLQTLSDESDEVVLKDLEVLAEIASSPAGQTDDPGAPDGPDLRVNHSELQVPTSGRANLLNPPSTKGLEGSPSTPTMNSYFYKFMINLLQTFSSERKLLEARGPFIIRQLCLLLNAENIFHSMADILLREEDLKFASTMVHTLNTILLTSTELFQLRNQLKDLQTPESQNLFCCLYRSWCHNPVTTVSLCFLTQNYRHAYDLIQKFGDLEVTVDFLTEVDKLVQLIECPIFT

Target ORF information:

RefSeq Version	XM_006530918.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Vac14 homolog (S. cerevisiae) (Vac14), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006530918.3

ORF Insert Sequence:

ATGAACCCAG AGAAGGATTT TGCGCCGCTC ACTCCCAACA TCGTAAGGGC CCTGAATGAT 
AAACTCTACG AAAAGCGGAA GGTTGCAGCG CTGGAGATCG AGAAGCTGGT GCGGGACTTT 
GTGGCCCAGA ACAACACCAT GCAAATCAAG CACGTGATCC AGACCCTGTC TCAGGAGTTT 
GCCCTGTCCC AGCACCCCCA CAGCCGGAAA GGGGGCCTCA TCGGTCTGGC AGCCTGCTCT 
ATTGCCCTAG GCAAGGACTC AGGGCTCTAC CTGAAGGAGC TGATAGAGCC AGTGCTGACC 
TGCTTTAATG ACGCAGACAG CAGGCTGCGC TACTACGCTT GTGAGGCCCT CTACAATATC 
GTCAAAGTGG CCCGAGGTGC CGTGCTGCCG CACTTCAATG TTCTCTTCGA TGGACTGAGT 
AAGTTGGCTG CTGACCCAGA CCCCAATGTG AAAAGTGGAT CTGAGCTCCT GGACCGCCTC 
TTAAAGGACA TTGTGACAGA AAGCAGCAAG TTTGACCTGG TGAGCTTCAT CCCCCTTTTG 
CGGGAGAGGA TTTACTCCAA CAACCAATAC GCCCGACAGT TCATCATCTC CTGGATCCTG 
GTTCTGGTGT CGGTGCCAGA CATTAACCTG CTGGATTACC TGCCAGAGAT CCTAGATGGG 
CTCTTCCAGA TCCTGGGTGA CAATGGCAAG GAGATTCGGA AAATGTGTGA GGTGGTTCTT 
GGAGAGTTCT TGAAAGAAAT TAAGAAGAAC CCATCCAGTG TGAAGTTTGC TGAGATGGCC 
AACATCCTGG TGATCCACTG CCAGACCACA GATGACCTGA TTCAGCTGAC GGCTATGTGC 
TGGATGCGGG AATTCATCCA GCTGGCGGGC CGGGTAATGC TGCCCTATTC CTCTGGGATC 
CTGACTGCTG TCCTGCCATG CTTGGCCTAT GATGACCGCA AGAAAAGTAT CAAGGAGGTG 
GCCAATGTGT GTAACCAGAG CTTGATGAAG CTGGTCACCC CTGAGGATGA TGAACCCGAT 
GAGCCAAAGT CAGTAGCACA GAAGCAGACC GAACCCAACC CTGAGGATTC CCTGCCAAAG 
CAGGAGGGGA CAGCCAGTGG AGGTCCAGGT TCCTGTGACT CCAGCTTTGG AAGTGGCATC 
AATGTCTTCA CCTCAGCCAA CACTGATAGG GCTCCAGTGA CCCTGCACCT GGACGGGATT 
GTTCAAGTCC TGAACTGTCA CCTTAGTGAC ACAACCATTG GGATGATGAC CAGGATTGCT 
GTTCTGAAGT GGCTCTACCA TCTCTACATC AAAACTCCTC GAAAGATGTT CCGGCACACA 
GACAGCCTCT TCCCCATCTT ACTTCAGACA TTATCTGACG AATCTGATGA GGTTGTCTTG 
AAGGATCTGG AAGTGTTGGC AGAAATTGCT TCCTCCCCTG CAGGCCAGAC GGATGACCCA 
GGTGCACCTG ATGGCCCTGA CCTCCGAGTC AACCACTCGG AGCTTCAGGT GCCCACCTCC 
GGCAGAGCCA ACCTACTAAA CCCTCCCAGT ACCAAAGGCT TAGAAGGCTC CCCCTCCACT 
CCCACCATGA ACTCCTACTT TTACAAGTTC ATGATCAACC TTCTACAGAC GTTCAGCAGT 
GAACGGAAGC TCCTGGAGGC CAGAGGCCCG TTCATCATCA GGCAGCTCTG CCTCCTGCTG 
AATGCAGAGA ACATCTTCCA TTCAATGGCA GACATCCTGC TTCGGGAGGA GGACCTCAAG 
TTCGCGTCCA CCATGGTGCA CACCCTCAAC ACCATCCTGC TCACCTCCAC CGAGCTCTTC 
CAGCTGAGGA ACCAGCTCAA GGACCTGCAG ACCCCGGAAA GCCAGAACCT CTTCTGCTGC 
CTGTACCGCT CCTGGTGCCA CAACCCAGTC ACCACCGTGT CCCTCTGCTT CCTCACCCAG 
AACTACCGCC ACGCCTATGA CCTCATCCAG AAGTTTGGGG ACCTGGAAGT CACAGTGGAT 
TTCCTCACAG AGGTGGACAA ACTCGTGCAG CTAATTGAGT GCCCCATCTT TACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A molecular mechanism to regulate lysosome motility for lysosome positioning and tubulation.
Nature cell biology18(4)404-17(2016 Apr)
Li X,Rydzewski N,Hider A,Zhang X,Yang J,Wang W,Gao Q,Cheng X,Xu H

PI(3,5)P2 biosynthesis regulates oligodendrocyte differentiation by intrinsic and extrinsic mechanisms.
eLife5(2016 03)
Mironova YA,Lenk GM,Lin JP,Lee SJ,Twiss JL,Vaccari I,Bolino A,Havton LA,Min SH,Abrams CS,Shrager P,Meisler MH,Giger RJ

Upregulation of Parkin in endophilin mutant mice.
The Journal of neuroscience : the official journal of the Society for Neuroscience34(49)16544-9(2014 Dec)
Cao M,Milosevic I,Giovedi S,De Camilli P

PIKfyve, a class III lipid kinase, is required for TLR-induced type I IFN production via modulation of ATF3.
Journal of immunology (Baltimore, Md. : 1950)192(7)3383-9(2014 Apr)
Cai X,Xu Y,Kim YM,Loureiro J,Huang Q

In vivo, Pikfyve generates PI(3,5)P2, which serves as both a signaling lipid and the major precursor for PI5P.
Proceedings of the National Academy of Sciences of the United States of America109(43)17472-7(2012 Oct)
Zolov SN,Bridges D,Zhang Y,Lee WW,Riehle E,Verma R,Lenk GM,Converso-Baran K,Weide T,Albin RL,Saltiel AR,Meisler MH,Russell MW,Weisman LS

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Vac14 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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