Fhod1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Fhod1
Entrez Gene ID	234686
Full Name	formin homology 2 domain containing 1
Synonyms	BC017144,FHOS,FHOS1
General protein information	Preferred Names formin homology 2 domain containing 1 Names FH1/FH2 domain-containing protein 1 formin homolog overexpressed in spleen 1 formin homology 2 domain-containing protein 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8\|8 D3

mRNA and Protein(s)

mRNA	Protein	Name
NM_177699.4	NP_808367.2	FH1/FH2 domain-containing protein 1
XM_030243503.1	XP_030099363.1	FH1/FH2 domain-containing protein 1 isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Fhod1	NP_808367.2
Rattus norvegicus (Norway rat)	Fhod1	NP_001178529.1
Danio rerio (zebrafish)	fhod1	XP_003199019.2

Gene Ontology
Bibliography

Related articles in PubMed

FHOD1 regulates cytoplasmic actin-based spindle migration for mouse oocyte asymmetric cell division.
Pan MH, Wang F, Lu Y, Tang F, Duan X, Zhang Y, Xiong B, Sun SC
Journal of cellular physiology233(3)2270-2278(2018 Mar)

Expression of multiple formins in adult tissues and during developmental stages of mouse brain.
Dutta P, Maiti S
Gene expression patterns : GEP19(1-2)52-9(2015 Sep-Nov)

The formin FHOD1 in cardiomyocytes.
Dwyer J, Pluess M, Iskratsch T, Dos Remedios CG, Ehler E
Anatomical record (Hoboken, N.J. : 2007)297(9)1560-70(2014 Sep)

FHOD1 interaction with nesprin-2G mediates TAN line formation and nuclear movement.
Kutscheidt S, Zhu R, Antoku S, Luxton GW, Stagljar I, Fackler OT, Gundersen GG
Nature cell biology16(7)708-15(2014 Jul)

FHOD1 is needed for directed forces and adhesion maturation during cell spreading and migration.
Iskratsch T, Yu CH, Mathur A, Liu S, St?venin V, Dwyer J, Hone J, Ehler E, Sheetz M
Developmental cell27(5)545-59(2013 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

FHOD1 is regulated by ROCK for cytoplasm actin assembly and spindle migration during mouse oocyte meiosis.
Title: FHOD1 regulates cytoplasmic actin-based spindle migration for mouse oocyte asymmetric cell division.

Report FODH1 expression in cardiomyocytes.
Title: The formin FHOD1 in cardiomyocytes.

We report here that a major formin in mouse fibroblasts, FHOD1, is recruited to integrin clusters, resulting in actin assembly.
Title: FHOD1 is needed for directed forces and adhesion maturation during cell spreading and migration.

Letter: G-protein coupled and ITAM receptor regulation of the formin FHOD1 through Rho kinase in platelets.
Title: G-protein coupled and ITAM receptor regulation of the formin FHOD1 through Rho kinase in platelets.

The following Fhod1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fhod1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu89823	XM_030243503.1 Latest version!	Mus musculus formin homology 2 domain containing 1 (Fhod1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu06671	NM_177699.4 Latest version!	Mus musculus formin homology 2 domain containing 1 (Fhod1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fhod1 ( XM_030243503.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030099363.1 Mus musculus formin homology 2 domain containing 1 (Fhod1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu89823
Clone ID Related Accession (Same CDS sequence)	XM_030243503.1
Accession Version	XM_030243503.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2679bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	FH1/FH2 domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCGC TGGTCCAACG TTTCTTGGGC ACCGCTGGCA CTGATGTTGA CCTGCGAACC 
CAGCTAACGC TCTATGAGAG TGCCCTTCGG TTAGAGGATG GAGATATGGA AGAGGCTGCA 
GCCGCCGCTG CTGCAGGTGG CCGGCGAGAG CGGCGGAAGC CATCCTCGGA GGAGGGCAAA 
AGGAGCCGAA GATCACTAGA AGGTGGAGGC TGCCCTGTGC GTGCCCCAGA ACCTGGCTCT 
ACAGGCTCCG CCTCACCAGT AGGCTCCACC CCCTCCACTG GCTCCGCCCC GCCTACAAAT 
CCAGCCTTCA GCTCTACTGG CCCAGCCTCT GGCCTTCTTC GAACCTCAGT GAACCTCTTT 
CCTACCATTT CCGTGGGGCC GTCAGTGGAC AGTTCCTGTG AGAGAAGCGT CTACAAAGCC 
CGGTTCCTGG AGAATGTGGC GGCAGCAGAG ACGGAGAAGC AGGCTGCTCT GGCCCAAGGC 
CGAGCGGAGA CGCTGGCTGG AGCCACGGTA GATGACACTG ATGGATCATC AGGCACAAGG 
GAACTGTGGG ACTCCCCAGA GCCAGCCTCT GCACCCAGGA CACCCCAGAG CCCTGTTTCC 
CGAATCCTGC TGCGCACCCA GCGGAGTCTT GAGCCAGAGC CCAAGAAGCC AGTGTCACCA 
CCAAGCCCTA AGGCTGAGCC TATCCAGGAG CCTCCCACCT GTGTCCCCAA GCTCTGCATT 
GGGGACTTGG ACTTCTCAGA CCTAGGGGAG GATGAAGACC AGGACACACT GAATGTGGAA 
TCTGTGGAGG CTGGAAAAGC ATCTCCCTTC CTGTCATCTC TATCGCCCTC ACTCTCTGGG 
GGTCCCCCTC CTCCGCCCCC ACCTCCTCCA CCCATCACAG GCTCCTGCCC CCCACCTCCG 
CCCCCACCTC TCCCCCCACC CGCCACAGGC TCCTGCCCTC CACCTCCGCC CCCACCTCCC 
CCACCCATCA TAGGCTCCTG CCCACCGCCT CCACCCCTGG CTGCTCCTTT TACCCACTCA 
GCACTTGACG GCCCAAGGCA CCCCACCAAA AGGAAGACAG TAAAACTTTT CTGGCGGGAA 
CTAAAGCTGA CTGGGGGCCC TGGGTGCTCT AGAAGCCGCT TTGGGCCTTG TCCTACCCTG 
TGGGCCTCGC TGGAACCCGT CTCGGTGGAC ACAGCCCGCC TGGAACACCT ATTTGAGTCC 
AGAGCCAAGG ATGTGCTACC AACCAAGAAA GCTGGTGAGG GCCGCCGGAC AATGACCGTA 
GTGCTGGACC CCAAGCGCAG CAATGCCATC AACATTGGCC TAACCACTCT GCCACCCGTG 
CACGTCATCA AGGCTGCCCT GCTCAACTTC GATGAGTTTG CTGTCAGCAA AGATGGCATT 
GAGAAACTGC TGACAATGAT GCCCACGGAG GAAGAGCGGC AGAAGATTGA GGAAGCCCAG 
CTGGCTAACC CCGATGTACC CCTCGGCCCC GCTGAGAATT TCCTGATGAC GCTTGCTTCC 
ATTGGAGGCC TGGCTGCGCG CCTACAGCTC TGGGCTTTCA AGCTGGACTA TGAAAGCATG 
GAGCGGGAAA TTGCAGAGCC ACTGTTTGAT CTGAAAGTGG GCATGGAACA GCTGGTACAC 
AATGCCACCT TCCGCTGTAT TCTGGCTACC CTTTTGGCTG TGGGCAACTT CCTCAATGGT 
TCCCAGAGCA GTGGCTTTGA GCTGAGCTAC CTGGAGAAGG TGTCAGAAGT GAAGGACACA 
GTGCGACGGC AGTCATTGCT CTATCATCTC TGCTCCCTGG TGCTCCAGAC CCGACCTGAT 
TCCTCTGACC TCTACTCAGA AATTCCTGCC CTCACCCGCT GTGCCAAGGT GGACTTTGAA 
CAGCTGACTG AGAACCTAGG GCAGCTGGAG TGCCGGAGCC AGGCTGCCGA GGACAGCCTC 
CGGAGCTTGG CTAAGCACGA GCTCTCCCCA GCTCTGCGTG CTCGCCTCAC CCACTTCTTG 
GCCCAGTGTA CCCGCCGGGT AGCCATGTTA AGAGTAGTGC ATCGCCGAGT CTGCAATAGG 
TTCCATGCCT TCCTGCTCTA CCTGGGCTAC ACCCCACAGG CAGCAAGGGA TGTACGCATC 
ATGCAGTTCT GCCACACACT GAGAGAGTTT GCCCTTGAGT ATCGGACTTG TCGGGAACGG 
GTACTGCAGC AGCAGCAGAA GCGGGCTACA TACCGTGAGC GCAACAAGAC CCGTGGTCGC 
ATGATTACCG AGACAGAGAA GTTCTCAGGT GTGGCTGGGG AGGCCCCCAA TAACCTGTCT 
GTCCCAGTGG CTGTGGGCAG CGGGCCAGGT CAGGGTGATA CTGACAATCA TGCCAGCATG 
AAGAGCCTGC TTACCAGCAG GCCGGAAGAT GCCACACACA GCCGACGCAG CAGAGGTATG 
GTCCAGAGCA GTTCCCCCGT CTCACACACA GCAGTGGGGC CCTCCGCTGC ATCCCCTGAG 
GAGACTGCAG CCTCCGGCTT ACCCACCGAC ACGTCAGATG AGATAATGGA CCTGCTGGTG 
CAGTCAGTTA CCAAGAGCGG TCCTAGAGCC TTAGCTGCTC GGGAGAGGAA GCGCTCTCGT 
GGCAACCGAA AGTCCTTGAG ACGGACACTG AAGAGTGGAC TTGGAGATGA CCTGGTGCAG 
GCACTGGGAC TAAGCAAAGC TCCTGGTCTA GAGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030099363.1
CDS	1127..3805
Translation	MEALVQRFLGTAGTDVDLRTQLTLYESALRLEDGDMEEAAAAAAAGGRRERRKPSSEEGKRSRRSLEGGGCPVRAPEPGSTGSASPVGSTPSTGSAPPTNPAFSSTGPASGLLRTSVNLFPTISVGPSVDSSCERSVYKARFLENVAAAETEKQAALAQGRAETLAGATVDDTDGSSGTRELWDSPEPASAPRTPQSPVSRILLRTQRSLEPEPKKPVSPPSPKAEPIQEPPTCVPKLCIGDLDFSDLGEDEDQDTLNVESVEAGKASPFLSSLSPSLSGGPPPPPPPPPPITGSCPPPPPPPLPPPATGSCPPPPPPPPPPIIGSCPPPPPLAAPFTHSALDGPRHPTKRKTVKLFWRELKLTGGPGCSRSRFGPCPTLWASLEPVSVDTARLEHLFESRAKDVLPTKKAGEGRRTMTVVLDPKRSNAINIGLTTLPPVHVIKAALLNFDEFAVSKDGIEKLLTMMPTEEERQKIEEAQLANPDVPLGPAENFLMTLASIGGLAARLQLWAFKLDYESMEREIAEPLFDLKVGMEQLVHNATFRCILATLLAVGNFLNGSQSSGFELSYLEKVSEVKDTVRRQSLLYHLCSLVLQTRPDSSDLYSEIPALTRCAKVDFEQLTENLGQLECRSQAAEDSLRSLAKHELSPALRARLTHFLAQCTRRVAMLRVVHRRVCNRFHAFLLYLGYTPQAARDVRIMQFCHTLREFALEYRTCRERVLQQQQKRATYRERNKTRGRMITETEKFSGVAGEAPNNLSVPVAVGSGPGQGDTDNHASMKSLLTSRPEDATHSRRSRGMVQSSSPVSHTAVGPSAASPEETAASGLPTDTSDEIMDLLVQSVTKSGPRALAARERKRSRGNRKSLRRTLKSGLGDDLVQALGLSKAPGLEV

Target ORF information:

RefSeq Version	XM_030243503.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus formin homology 2 domain containing 1 (Fhod1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030243503.1

ORF Insert Sequence:

ATGGAAGCGC TGGTCCAACG TTTCTTGGGC ACCGCTGGCA CTGATGTTGA CCTGCGAACC 
CAGCTAACGC TCTATGAGAG TGCCCTTCGG TTAGAGGATG GAGATATGGA AGAGGCTGCA 
GCCGCCGCTG CTGCAGGTGG CCGGCGAGAG CGGCGGAAGC CATCCTCGGA GGAGGGCAAA 
AGGAGCCGAA GATCACTAGA AGGTGGAGGC TGCCCTGTGC GTGCCCCAGA ACCTGGCTCT 
ACAGGCTCCG CCTCACCAGT AGGCTCCACC CCCTCCACTG GCTCCGCCCC GCCTACAAAT 
CCAGCCTTCA GCTCTACTGG CCCAGCCTCT GGCCTTCTTC GAACCTCAGT GAACCTCTTT 
CCTACCATTT CCGTGGGGCC GTCAGTGGAC AGTTCCTGTG AGAGAAGCGT CTACAAAGCC 
CGGTTCCTGG AGAATGTGGC GGCAGCAGAG ACGGAGAAGC AGGCTGCTCT GGCCCAAGGC 
CGAGCGGAGA CGCTGGCTGG AGCCACGGTA GATGACACTG ATGGATCATC AGGCACAAGG 
GAACTGTGGG ACTCCCCAGA GCCAGCCTCT GCACCCAGGA CACCCCAGAG CCCTGTTTCC 
CGAATCCTGC TGCGCACCCA GCGGAGTCTT GAGCCAGAGC CCAAGAAGCC AGTGTCACCA 
CCAAGCCCTA AGGCTGAGCC TATCCAGGAG CCTCCCACCT GTGTCCCCAA GCTCTGCATT 
GGGGACTTGG ACTTCTCAGA CCTAGGGGAG GATGAAGACC AGGACACACT GAATGTGGAA 
TCTGTGGAGG CTGGAAAAGC ATCTCCCTTC CTGTCATCTC TATCGCCCTC ACTCTCTGGG 
GGTCCCCCTC CTCCGCCCCC ACCTCCTCCA CCCATCACAG GCTCCTGCCC CCCACCTCCG 
CCCCCACCTC TCCCCCCACC CGCCACAGGC TCCTGCCCTC CACCTCCGCC CCCACCTCCC 
CCACCCATCA TAGGCTCCTG CCCACCGCCT CCACCCCTGG CTGCTCCTTT TACCCACTCA 
GCACTTGACG GCCCAAGGCA CCCCACCAAA AGGAAGACAG TAAAACTTTT CTGGCGGGAA 
CTAAAGCTGA CTGGGGGCCC TGGGTGCTCT AGAAGCCGCT TTGGGCCTTG TCCTACCCTG 
TGGGCCTCGC TGGAACCCGT CTCGGTGGAC ACAGCCCGCC TGGAACACCT ATTTGAGTCC 
AGAGCCAAGG ATGTGCTACC AACCAAGAAA GCTGGTGAGG GCCGCCGGAC AATGACCGTA 
GTGCTGGACC CCAAGCGCAG CAATGCCATC AACATTGGCC TAACCACTCT GCCACCCGTG 
CACGTCATCA AGGCTGCCCT GCTCAACTTC GATGAGTTTG CTGTCAGCAA AGATGGCATT 
GAGAAACTGC TGACAATGAT GCCCACGGAG GAAGAGCGGC AGAAGATTGA GGAAGCCCAG 
CTGGCTAACC CCGATGTACC CCTCGGCCCC GCTGAGAATT TCCTGATGAC GCTTGCTTCC 
ATTGGAGGCC TGGCTGCGCG CCTACAGCTC TGGGCTTTCA AGCTGGACTA TGAAAGCATG 
GAGCGGGAAA TTGCAGAGCC ACTGTTTGAT CTGAAAGTGG GCATGGAACA GCTGGTACAC 
AATGCCACCT TCCGCTGTAT TCTGGCTACC CTTTTGGCTG TGGGCAACTT CCTCAATGGT 
TCCCAGAGCA GTGGCTTTGA GCTGAGCTAC CTGGAGAAGG TGTCAGAAGT GAAGGACACA 
GTGCGACGGC AGTCATTGCT CTATCATCTC TGCTCCCTGG TGCTCCAGAC CCGACCTGAT 
TCCTCTGACC TCTACTCAGA AATTCCTGCC CTCACCCGCT GTGCCAAGGT GGACTTTGAA 
CAGCTGACTG AGAACCTAGG GCAGCTGGAG TGCCGGAGCC AGGCTGCCGA GGACAGCCTC 
CGGAGCTTGG CTAAGCACGA GCTCTCCCCA GCTCTGCGTG CTCGCCTCAC CCACTTCTTG 
GCCCAGTGTA CCCGCCGGGT AGCCATGTTA AGAGTAGTGC ATCGCCGAGT CTGCAATAGG 
TTCCATGCCT TCCTGCTCTA CCTGGGCTAC ACCCCACAGG CAGCAAGGGA TGTACGCATC 
ATGCAGTTCT GCCACACACT GAGAGAGTTT GCCCTTGAGT ATCGGACTTG TCGGGAACGG 
GTACTGCAGC AGCAGCAGAA GCGGGCTACA TACCGTGAGC GCAACAAGAC CCGTGGTCGC 
ATGATTACCG AGACAGAGAA GTTCTCAGGT GTGGCTGGGG AGGCCCCCAA TAACCTGTCT 
GTCCCAGTGG CTGTGGGCAG CGGGCCAGGT CAGGGTGATA CTGACAATCA TGCCAGCATG 
AAGAGCCTGC TTACCAGCAG GCCGGAAGAT GCCACACACA GCCGACGCAG CAGAGGTATG 
GTCCAGAGCA GTTCCCCCGT CTCACACACA GCAGTGGGGC CCTCCGCTGC ATCCCCTGAG 
GAGACTGCAG CCTCCGGCTT ACCCACCGAC ACGTCAGATG AGATAATGGA CCTGCTGGTG 
CAGTCAGTTA CCAAGAGCGG TCCTAGAGCC TTAGCTGCTC GGGAGAGGAA GCGCTCTCGT 
GGCAACCGAA AGTCCTTGAG ACGGACACTG AAGAGTGGAC TTGGAGATGA CCTGGTGCAG 
GCACTGGGAC TAAGCAAAGC TCCTGGTCTA GAGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fhod1 ( NM_177699.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_808367.2 Mus musculus formin homology 2 domain containing 1 (Fhod1), mRNA.

Price & Availability↑

CloneID	OMu06671
Clone ID Related Accession (Same CDS sequence)	NM_177699.4
Accession Version	NM_177699.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	FH1/FH2 domain-containing protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB858085.1, AB041045.1 and AW540395.2. On Nov 28, 2009 this sequence version replaced NM_177699.3. ##Evidence-Data-START## Transcript exon combination :: BC060654.1, AK030737.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGCG AGGAAGAGCG CGGGGATGGG GACCCAGTAT CCGTGGTAAC TGTGAGAGTG 
CAGTACCTGG AAGACACCGA CCCTTTCGCT TGTGCCAACT TCCCGGAACC ACGCCGGGCC 
CCCACCTGCA GCCTGGACGG GGCTCTGCCC CTGAGTGCGC AGATCCCTGC TCTGCACCGA 
CTCCTGGGGG CGCCTCTTAA GCTGGAGGAC TGTGCATTGC AAGTGTCTCC CTCCGGATAC 
TACCTGGACC CGGAGCTGTC CCTAGAAGAA CAGCGGGAGA TGCTGGAGGG TTTCTATGAA 
GAGATCAGCA AAGGGCGGAA GCCCACGTTA ATCCTGCGGA CCCAGCTCTC CGTGAGGGTC 
AATGCTATCT TGGAAAAGCT GTATGGCTCC AGTGGCCCCG AGCTCCGCCG ATCTCTCTTC 
TCATTAAAGC AGATATTCCA GGAAGACAAG GACCTGGTGC CCGAATTCGT GCACTCGGAG 
GGTCTGAGTT GCCTGATCCG CGTGGGTGCT GCTGCAGACC ACAACTACCA GAGCTACATC 
CTTCGAGCCC TGGGCCAGCT GATGCTGTTT GTGGATGGGA TGCTGGGGGT GGTGGCCCAC 
AGCGAGACCG TGCAGTGGCT GTATACCCTG TGTGCTAGCC TGTCCCGCTT GGTGGTAAAG 
ACAGCCCTGA AGCTGCTGCT GGTGTTTGTG GAGTATTCCG AGAACAACGC GCCGCTGTTC 
ATCCAGGCAG TCAACGCAGT AGCCAGTGCC ACCGGTACTC TTCCCTGGGC CAACTTGGTG 
TCCATCCTGG AGGAGAAGAA TGGAGCTGAT GCAGAGTTGT TGGTGTACAC TGTCACTCTC 
ATTAACAAGA CGCTGGCGGC ACTCCCAGAC CAGGACTCCT TCTATGATGT GACAGATGCT 
CTGGAGCAGC AGGGCATGGA AGCGCTGGTC CAACGTTTCT TGGGCACCGC TGGCACTGAT 
GTTGACCTGC GAACCCAGCT AACGCTCTAT GAGAGTGCCC TTCGGTTAGA GGATGGAGAT 
ATGGAAGAGG CTGCAGCCGC CGCTGCTGCA GGTGGCCGGC GAGAGCGGCG GAAGCCATCC 
TCGGAGGAGG GCAAAAGGAG CCGAAGATCA CTAGAAGGTG GAGGCTGCCC TGTGCGTGCC 
CCAGAACCTG GCTCTACAGG CTCCGCCTCA CCAGTAGGCT CCACCCCCTC CACTGGCTCC 
GCCCCGCCTA CAAATCCAGC CTTCAGCTCT ACTGGCCCAG CCTCTGGCCT TCTTCGAACC 
TCAGTGAACC TCTTTCCTAC CATTTCCGTG GGGCCGTCAG TGGACAGTTC CTGTGAGAGA 
AGCGTCTACA AAGCCCGGTT CCTGGAGAAT GTGGCGGCAG CAGAGACGGA GAAGCAGGCT 
GCTCTGGCCC AAGGCCGAGC GGAGACGCTG GCTGGAGCCA CGGTAGATGA CACTGATGGA 
TCATCAGGCA CAAGGGAACT GTGGGACTCC CCAGAGCCAG CCTCTGCACC CAGGACACCC 
CAGAGCCCTG TTTCCCGAAT CCTGCTGCGC ACCCAGCGGA GTCTTGAGCC AGAGCCCAAG 
AAGCCAGTGT CACCACCAAG CCCTAAGGCT GAGCCTATCC AGGAGCCTCC CACCTGTGTC 
CCCAAGCTCT GCATTGGGGA CTTGGACTTC TCAGACCTAG GGGAGGATGA AGACCAGGAC 
ACACTGAATG TGGAATCTGT GGAGGCTGGA AAAGCATCTC CCTTCCTGTC ATCTCTATCG 
CCCTCACTCT CTGGGGGTCC CCCTCCTCCG CCCCCACCTC CTCCACCCAT CACAGGCTCC 
TGCCCCCCAC CTCCGCCCCC ACCTCTCCCC CCACCCGCCA CAGGCTCCTG CCCTCCACCT 
CCGCCCCCAC CTCCCCCACC CATCATAGGC TCCTGCCCAC CGCCTCCACC CCTGGCTGCT 
CCTTTTACCC ACTCAGCACT TGACGGCCCA AGGCACCCCA CCAAAAGGAA GACAGTAAAA 
CTTTTCTGGC GGGAACTAAA GCTGACTGGG GGCCCTGGGT GCTCTAGAAG CCGCTTTGGG 
CCTTGTCCTA CCCTGTGGGC CTCGCTGGAA CCCGTCTCGG TGGACACAGC CCGCCTGGAA 
CACCTATTTG AGTCCAGAGC CAAGGATGTG CTACCAACCA AGAAAGCTGG TGAGGGCCGC 
CGGACAATGA CCGTAGTGCT GGACCCCAAG CGCAGCAATG CCATCAACAT TGGCCTAACC 
ACTCTGCCAC CCGTGCACGT CATCAAGGCT GCCCTGCTCA ACTTCGATGA GTTTGCTGTC 
AGCAAAGATG GCATTGAGAA ACTGCTGACA ATGATGCCCA CGGAGGAAGA GCGGCAGAAG 
ATTGAGGAAG CCCAGCTGGC TAACCCCGAT GTACCCCTCG GCCCCGCTGA GAATTTCCTG 
ATGACGCTTG CTTCCATTGG AGGCCTGGCT GCGCGCCTAC AGCTCTGGGC TTTCAAGCTG 
GACTATGAAA GCATGGAGCG GGAAATTGCA GAGCCACTGT TTGATCTGAA AGTGGGCATG 
GAACAGCTGG TACACAATGC CACCTTCCGC TGTATTCTGG CTACCCTTTT GGCTGTGGGC 
AACTTCCTCA ATGGTTCCCA GAGCAGTGGC TTTGAGCTGA GCTACCTGGA GAAGGTGTCA 
GAAGTGAAGG ACACAGTGCG ACGGCAGTCA TTGCTCTATC ATCTCTGCTC CCTGGTGCTC 
CAGACCCGAC CTGATTCCTC TGACCTCTAC TCAGAAATTC CTGCCCTCAC CCGCTGTGCC 
AAGGTGGACT TTGAACAGCT GACTGAGAAC CTAGGGCAGC TGGAGTGCCG GAGCCAGGCT 
GCCGAGGACA GCCTCCGGAG CTTGGCTAAG CACGAGCTCT CCCCAGCTCT GCGTGCTCGC 
CTCACCCACT TCTTGGCCCA GTGTACCCGC CGGGTAGCCA TGTTAAGAGT AGTGCATCGC 
CGAGTCTGCA ATAGGTTCCA TGCCTTCCTG CTCTACCTGG GCTACACCCC ACAGGCAGCA 
AGGGATGTAC GCATCATGCA GTTCTGCCAC ACACTGAGAG AGTTTGCCCT TGAGTATCGG 
ACTTGTCGGG AACGGGTACT GCAGCAGCAG CAGAAGCGGG CTACATACCG TGAGCGCAAC 
AAGACCCGTG GTCGCATGAT TACCGAGACA GAGAAGTTCT CAGGTGTGGC TGGGGAGGCC 
CCCAATAACC TGTCTGTCCC AGTGGCTGTG GGCAGCGGGC CAGGTCAGGG TGATACTGAC 
AATCATGCCA GCATGAAGAG CCTGCTTACC AGCAGGCCGG AAGATGCCAC ACACAGCCGA 
CGCAGCAGAG GTATGGTCCA GAGCAGTTCC CCCGTCTCAC ACACAGCAGT GGGGCCCTCC 
GCTGCATCCC CTGAGGAGAC TGCAGCCTCC GGCTTACCCA CCGACACGTC AGATGAGATA 
ATGGACCTGC TGGTGCAGTC AGTTACCAAG AGCGGTCCTA GAGCCTTAGC TGCTCGGGAG 
AGGAAGCGCT CTCGTGGCAA CCGAAAGTCC TTGAGACGGA CACTGAAGAG TGGACTTGGA 
GATGACCTGG TGCAGGCACT GGGACTAAGC AAAGCTCCTG GTCTAGAGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_808367.2
CDS	118..3711
Translation	MAGEEERGDGDPVSVVTVRVQYLEDTDPFACANFPEPRRAPTCSLDGALPLSAQIPALHRLLGAPLKLEDCALQVSPSGYYLDPELSLEEQREMLEGFYEEISKGRKPTLILRTQLSVRVNAILEKLYGSSGPELRRSLFSLKQIFQEDKDLVPEFVHSEGLSCLIRVGAAADHNYQSYILRALGQLMLFVDGMLGVVAHSETVQWLYTLCASLSRLVVKTALKLLLVFVEYSENNAPLFIQAVNAVASATGTLPWANLVSILEEKNGADAELLVYTVTLINKTLAALPDQDSFYDVTDALEQQGMEALVQRFLGTAGTDVDLRTQLTLYESALRLEDGDMEEAAAAAAAGGRRERRKPSSEEGKRSRRSLEGGGCPVRAPEPGSTGSASPVGSTPSTGSAPPTNPAFSSTGPASGLLRTSVNLFPTISVGPSVDSSCERSVYKARFLENVAAAETEKQAALAQGRAETLAGATVDDTDGSSGTRELWDSPEPASAPRTPQSPVSRILLRTQRSLEPEPKKPVSPPSPKAEPIQEPPTCVPKLCIGDLDFSDLGEDEDQDTLNVESVEAGKASPFLSSLSPSLSGGPPPPPPPPPPITGSCPPPPPPPLPPPATGSCPPPPPPPPPPIIGSCPPPPPLAAPFTHSALDGPRHPTKRKTVKLFWRELKLTGGPGCSRSRFGPCPTLWASLEPVSVDTARLEHLFESRAKDVLPTKKAGEGRRTMTVVLDPKRSNAINIGLTTLPPVHVIKAALLNFDEFAVSKDGIEKLLTMMPTEEERQKIEEAQLANPDVPLGPAENFLMTLASIGGLAARLQLWAFKLDYESMEREIAEPLFDLKVGMEQLVHNATFRCILATLLAVGNFLNGSQSSGFELSYLEKVSEVKDTVRRQSLLYHLCSLVLQTRPDSSDLYSEIPALTRCAKVDFEQLTENLGQLECRSQAAEDSLRSLAKHELSPALRARLTHFLAQCTRRVAMLRVVHRRVCNRFHAFLLYLGYTPQAARDVRIMQFCHTLREFALEYRTCRERVLQQQQKRATYRERNKTRGRMITETEKFSGVAGEAPNNLSVPVAVGSGPGQGDTDNHASMKSLLTSRPEDATHSRRSRGMVQSSSPVSHTAVGPSAASPEETAASGLPTDTSDEIMDLLVQSVTKSGPRALAARERKRSRGNRKSLRRTLKSGLGDDLVQALGLSKAPGLEV

Target ORF information:

RefSeq Version	NM_177699.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus formin homology 2 domain containing 1 (Fhod1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177699.4

ORF Insert Sequence:

ATGGCGGGCG AGGAAGAGCG CGGGGATGGG GACCCAGTAT CCGTGGTAAC TGTGAGAGTG 
CAGTACCTGG AAGACACCGA CCCTTTCGCT TGTGCCAACT TCCCGGAACC ACGCCGGGCC 
CCCACCTGCA GCCTGGACGG GGCTCTGCCC CTGAGTGCGC AGATCCCTGC TCTGCACCGA 
CTCCTGGGGG CGCCTCTTAA GCTGGAGGAC TGTGCATTGC AAGTGTCTCC CTCCGGATAC 
TACCTGGACC CGGAGCTGTC CCTAGAAGAA CAGCGGGAGA TGCTGGAGGG TTTCTATGAA 
GAGATCAGCA AAGGGCGGAA GCCCACGTTA ATCCTGCGGA CCCAGCTCTC CGTGAGGGTC 
AATGCTATCT TGGAAAAGCT GTATGGCTCC AGTGGCCCCG AGCTCCGCCG ATCTCTCTTC 
TCATTAAAGC AGATATTCCA GGAAGACAAG GACCTGGTGC CCGAATTCGT GCACTCGGAG 
GGTCTGAGTT GCCTGATCCG CGTGGGTGCT GCTGCAGACC ACAACTACCA GAGCTACATC 
CTTCGAGCCC TGGGCCAGCT GATGCTGTTT GTGGATGGGA TGCTGGGGGT GGTGGCCCAC 
AGCGAGACCG TGCAGTGGCT GTATACCCTG TGTGCTAGCC TGTCCCGCTT GGTGGTAAAG 
ACAGCCCTGA AGCTGCTGCT GGTGTTTGTG GAGTATTCCG AGAACAACGC GCCGCTGTTC 
ATCCAGGCAG TCAACGCAGT AGCCAGTGCC ACCGGTACTC TTCCCTGGGC CAACTTGGTG 
TCCATCCTGG AGGAGAAGAA TGGAGCTGAT GCAGAGTTGT TGGTGTACAC TGTCACTCTC 
ATTAACAAGA CGCTGGCGGC ACTCCCAGAC CAGGACTCCT TCTATGATGT GACAGATGCT 
CTGGAGCAGC AGGGCATGGA AGCGCTGGTC CAACGTTTCT TGGGCACCGC TGGCACTGAT 
GTTGACCTGC GAACCCAGCT AACGCTCTAT GAGAGTGCCC TTCGGTTAGA GGATGGAGAT 
ATGGAAGAGG CTGCAGCCGC CGCTGCTGCA GGTGGCCGGC GAGAGCGGCG GAAGCCATCC 
TCGGAGGAGG GCAAAAGGAG CCGAAGATCA CTAGAAGGTG GAGGCTGCCC TGTGCGTGCC 
CCAGAACCTG GCTCTACAGG CTCCGCCTCA CCAGTAGGCT CCACCCCCTC CACTGGCTCC 
GCCCCGCCTA CAAATCCAGC CTTCAGCTCT ACTGGCCCAG CCTCTGGCCT TCTTCGAACC 
TCAGTGAACC TCTTTCCTAC CATTTCCGTG GGGCCGTCAG TGGACAGTTC CTGTGAGAGA 
AGCGTCTACA AAGCCCGGTT CCTGGAGAAT GTGGCGGCAG CAGAGACGGA GAAGCAGGCT 
GCTCTGGCCC AAGGCCGAGC GGAGACGCTG GCTGGAGCCA CGGTAGATGA CACTGATGGA 
TCATCAGGCA CAAGGGAACT GTGGGACTCC CCAGAGCCAG CCTCTGCACC CAGGACACCC 
CAGAGCCCTG TTTCCCGAAT CCTGCTGCGC ACCCAGCGGA GTCTTGAGCC AGAGCCCAAG 
AAGCCAGTGT CACCACCAAG CCCTAAGGCT GAGCCTATCC AGGAGCCTCC CACCTGTGTC 
CCCAAGCTCT GCATTGGGGA CTTGGACTTC TCAGACCTAG GGGAGGATGA AGACCAGGAC 
ACACTGAATG TGGAATCTGT GGAGGCTGGA AAAGCATCTC CCTTCCTGTC ATCTCTATCG 
CCCTCACTCT CTGGGGGTCC CCCTCCTCCG CCCCCACCTC CTCCACCCAT CACAGGCTCC 
TGCCCCCCAC CTCCGCCCCC ACCTCTCCCC CCACCCGCCA CAGGCTCCTG CCCTCCACCT 
CCGCCCCCAC CTCCCCCACC CATCATAGGC TCCTGCCCAC CGCCTCCACC CCTGGCTGCT 
CCTTTTACCC ACTCAGCACT TGACGGCCCA AGGCACCCCA CCAAAAGGAA GACAGTAAAA 
CTTTTCTGGC GGGAACTAAA GCTGACTGGG GGCCCTGGGT GCTCTAGAAG CCGCTTTGGG 
CCTTGTCCTA CCCTGTGGGC CTCGCTGGAA CCCGTCTCGG TGGACACAGC CCGCCTGGAA 
CACCTATTTG AGTCCAGAGC CAAGGATGTG CTACCAACCA AGAAAGCTGG TGAGGGCCGC 
CGGACAATGA CCGTAGTGCT GGACCCCAAG CGCAGCAATG CCATCAACAT TGGCCTAACC 
ACTCTGCCAC CCGTGCACGT CATCAAGGCT GCCCTGCTCA ACTTCGATGA GTTTGCTGTC 
AGCAAAGATG GCATTGAGAA ACTGCTGACA ATGATGCCCA CGGAGGAAGA GCGGCAGAAG 
ATTGAGGAAG CCCAGCTGGC TAACCCCGAT GTACCCCTCG GCCCCGCTGA GAATTTCCTG 
ATGACGCTTG CTTCCATTGG AGGCCTGGCT GCGCGCCTAC AGCTCTGGGC TTTCAAGCTG 
GACTATGAAA GCATGGAGCG GGAAATTGCA GAGCCACTGT TTGATCTGAA AGTGGGCATG 
GAACAGCTGG TACACAATGC CACCTTCCGC TGTATTCTGG CTACCCTTTT GGCTGTGGGC 
AACTTCCTCA ATGGTTCCCA GAGCAGTGGC TTTGAGCTGA GCTACCTGGA GAAGGTGTCA 
GAAGTGAAGG ACACAGTGCG ACGGCAGTCA TTGCTCTATC ATCTCTGCTC CCTGGTGCTC 
CAGACCCGAC CTGATTCCTC TGACCTCTAC TCAGAAATTC CTGCCCTCAC CCGCTGTGCC 
AAGGTGGACT TTGAACAGCT GACTGAGAAC CTAGGGCAGC TGGAGTGCCG GAGCCAGGCT 
GCCGAGGACA GCCTCCGGAG CTTGGCTAAG CACGAGCTCT CCCCAGCTCT GCGTGCTCGC 
CTCACCCACT TCTTGGCCCA GTGTACCCGC CGGGTAGCCA TGTTAAGAGT AGTGCATCGC 
CGAGTCTGCA ATAGGTTCCA TGCCTTCCTG CTCTACCTGG GCTACACCCC ACAGGCAGCA 
AGGGATGTAC GCATCATGCA GTTCTGCCAC ACACTGAGAG AGTTTGCCCT TGAGTATCGG 
ACTTGTCGGG AACGGGTACT GCAGCAGCAG CAGAAGCGGG CTACATACCG TGAGCGCAAC 
AAGACCCGTG GTCGCATGAT TACCGAGACA GAGAAGTTCT CAGGTGTGGC TGGGGAGGCC 
CCCAATAACC TGTCTGTCCC AGTGGCTGTG GGCAGCGGGC CAGGTCAGGG TGATACTGAC 
AATCATGCCA GCATGAAGAG CCTGCTTACC AGCAGGCCGG AAGATGCCAC ACACAGCCGA 
CGCAGCAGAG GTATGGTCCA GAGCAGTTCC CCCGTCTCAC ACACAGCAGT GGGGCCCTCC 
GCTGCATCCC CTGAGGAGAC TGCAGCCTCC GGCTTACCCA CCGACACGTC AGATGAGATA 
ATGGACCTGC TGGTGCAGTC AGTTACCAAG AGCGGTCCTA GAGCCTTAGC TGCTCGGGAG 
AGGAAGCGCT CTCGTGGCAA CCGAAAGTCC TTGAGACGGA CACTGAAGAG TGGACTTGGA 
GATGACCTGG TGCAGGCACT GGGACTAAGC AAAGCTCCTG GTCTAGAGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

FHOD1 regulates cytoplasmic actin-based spindle migration for mouse oocyte asymmetric cell division.
Journal of cellular physiology233(3)2270-2278(2018 Mar)
Pan MH,Wang F,Lu Y,Tang F,Duan X,Zhang Y,Xiong B,Sun SC

Expression of multiple formins in adult tissues and during developmental stages of mouse brain.
Gene expression patterns : GEP19(1-2)52-9(2015 Sep-Nov)
Dutta P,Maiti S

The formin FHOD1 in cardiomyocytes.
Anatomical record (Hoboken, N.J. : 2007)297(9)1560-70(2014 Sep)
Dwyer J,Pluess M,Iskratsch T,Dos Remedios CG,Ehler E

FHOD1 interaction with nesprin-2G mediates TAN line formation and nuclear movement.
Nature cell biology16(7)708-15(2014 Jul)
Kutscheidt S,Zhu R,Antoku S,Luxton GW,Stagljar I,Fackler OT,Gundersen GG

FHOD1 is needed for directed forces and adhesion maturation during cell spreading and migration.
Developmental cell27(5)545-59(2013 Dec)
Iskratsch T,Yu CH,Mathur A,Liu S,St?venin V,Dwyer J,Hone J,Ehler E,Sheetz M

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Fhod1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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