gRNAs
The following Smg1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Smg1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OMu14540 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001031814.1 | |
| Accession Version | NM_001031814.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 10977bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-09-21 | |
| Organism | Mus musculus(house mouse) | |
| Product | serine/threonine-protein kinase SMG1 | |
| Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC132432.4 and AC131788.3. On Aug 18, 2005 this sequence version replaced XM_620559.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## | |
1 | ATGAGCCGCA GAGCCCCGGG GTCTCGGCTG AGCAGCGGCG GCGGCGGCAC CAAGTACCCG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001026984.1 |
| CDS | 348..11324 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001031814.1 |
1 | ATGAGCCGCA GAGCCCCGGG GTCTCGGCTG AGCAGCGGCG GCGGCGGCAC CAAGTACCCG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu39525 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006507677.3 , XM_006507677.2 | |
| Accession Version | XM_006507677.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 10974bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-08-07 | |
| Organism | Mus musculus(house mouse) | |
| Product | serine/threonine-protein kinase SMG1 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006507677.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGAGCCGCA GAGCCCCGGG GTCTCGGCTG AGCAGCGGCG GCGGCGGCAC CAAGTACCCG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006507740.1 |
| CDS | 1148..12121 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006507677.3 |
1 | ATGAGCCGCA GAGCCCCGGG GTCTCGGCTG AGCAGCGGCG GCGGCGGCAC CAAGTACCCG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu39525 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006507677.3 , XM_006507677.2 | |
| Accession Version | XM_006507677.2 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 10974bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-06-21 | |
| Organism | Mus musculus(house mouse) | |
| Product | serine/threonine-protein kinase SMG1 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006507677.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGAGCCGCA GAGCCCCGGG GTCTCGGCTG AGCAGCGGCG GCGGCGGCAC CAAGTACCCG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006507740.1 |
| CDS | 368..11341 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006507677.2 |
1 | ATGAGCCGCA GAGCCCCGGG GTCTCGGCTG AGCAGCGGCG GCGGCGGCAC CAAGTACCCG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu39526 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006507678.3 , XM_006507678.2 | |
| Accession Version | XM_006507678.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 10905bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-08-07 | |
| Organism | Mus musculus(house mouse) | |
| Product | serine/threonine-protein kinase SMG1 isoform X2 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006507678.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGCGATTTT TGAGAACTGA TAGTGCATCA GCCGACCCAG ATACTTTAAA ATACTCCTCA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006507741.1 |
| CDS | 503..11407 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006507678.3 |
1 | ATGCGATTTT TGAGAACTGA TAGTGCATCA GCCGACCCAG ATACTTTAAA ATACTCCTCA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu39526 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006507678.3 , XM_006507678.2 | |
| Accession Version | XM_006507678.2 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 10905bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-06-21 | |
| Organism | Mus musculus(house mouse) | |
| Product | serine/threonine-protein kinase SMG1 isoform X2 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006507678.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGCGATTTT TGAGAACTGA TAGTGCATCA GCCGACCCAG ATACTTTAAA ATACTCCTCA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006507741.1 |
| CDS | 498..11402 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006507678.2 |
1 | ATGCGATTTT TGAGAACTGA TAGTGCATCA GCCGACCCAG ATACTTTAAA ATACTCCTCA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu89464 | |
| Clone ID Related Accession (Same CDS sequence) | XM_030242506.1 | |
| Accession Version | XM_030242506.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 10902bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-08-07 | |
| Organism | Mus musculus(house mouse) | |
| Product | serine/threonine-protein kinase SMG1 isoform X3 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGCGATTTT TGAGAACTGA TAGTGCATCA GCCGACCCAG ATACTTTAAA ATACTCCTCA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_030098366.1 |
| CDS | 464..11365 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_030242506.1 |
1 | ATGCGATTTT TGAGAACTGA TAGTGCATCA GCCGACCCAG ATACTTTAAA ATACTCCTCA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
A Role for the Nonsense-Mediated mRNA Decay Pathway in Maintaining Genome Stability in |
Smg6/Est1 licenses embryonic stem cell differentiation via nonsense-mediated mRNA decay. |
SMG1 regulates adipogenesis via targeting of staufen1-mediated mRNA decay. |
Smg1 haploinsufficiency predisposes to tumor formation and inflammation. |
