Rsf1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rsf1
Entrez Gene ID	233532
Full Name	remodeling and spacing factor 1
Synonyms	4832420A03Rik,C030033M12Rik,Gm164,Hbxap,XAP8,p325
General protein information	Preferred Names remodeling and spacing factor 1 Names remodeling and spacing factor 1 hepatitis B virus x associated protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 E1

mRNA and Protein(s)

mRNA	Protein	Name
XM_011241735.3	XP_011240037.1	remodeling and spacing factor 1 isoform X1
NM_001081267.2	NP_001074736.1	remodeling and spacing factor 1
XM_011241735.2	XP_011240037.1	remodeling and spacing factor 1 isoform X1

Pathways from BioSystems

Homologies

	RSF1	XP_001091378.1
Canis lupus familiaris (dog)	RSF1	XP_534002.2
Rattus norvegicus (Norway rat)	Rsf1	XP_218939.4
Homo sapiens (human)	RSF1	NP_057662.3
Gallus gallus (chicken)	RSF1	XP_424124.3
Danio rerio (zebrafish)	si:dkey-21n12.1	XP_005169237.1
Bos taurus (cattle)	RSF1	XP_002699109.1
Mus musculus (house mouse)	Rsf1	NP_001074736.1
Pan troglodytes (chimpanzee)	RSF1	XP_001175061.2

Gene Ontology
Bibliography

Related articles in PubMed

Role of remodeling and spacing factor 1 in histone H2A ubiquitination-mediated gene silencing.
Zhang Z, Jones AE, Wu W, Kim J, Kang Y, Bi X, Gu Y, Popov IK, Renfrow MB, Vassylyeva MN, Vassylyev DG, Giles KE, Chen D, Kumar A, Fan Y, Tong Y, Liu CF, An W, Chang C, Luo J, Chow LT, Wang H
Proceedings of the National Academy of Sciences of the United States of America114(38)E7949-E7958(2017 Sep)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Specification of jaw identity by the Hand2 transcription factor.
Funato N, Kokubo H, Nakamura M, Yanagisawa H, Saga Y
Scientific reports628405(2016 Jun)

Specific inhibition of gene expression and transactivation functions of hepatitis B virus X protein and c-myc by small interfering RNAs.
Hung L, Kumar V
FEBS letters560(1-3)210-4(2004 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Small interfering RNAs have specific inhibition of Hbxap expression and transactivation.
Title: Specific inhibition of gene expression and transactivation functions of hepatitis B virus X protein and c-myc by small interfering RNAs.

The following Rsf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rsf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu73683	XM_011241735.3 Latest version!	Mus musculus remodeling and spacing factor 1 (Rsf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu01993	NM_001081267.2 Latest version!	Mus musculus remodeling and spacing factor 1 (Rsf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
View Old Accession Versions >>
OMu73683	XM_011241735.2	Mus musculus remodeling and spacing factor 1 (Rsf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rsf1 ( XM_011241735.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011240037.1 Mus musculus remodeling and spacing factor 1 (Rsf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu73683
Clone ID Related Accession (Same CDS sequence)	XM_011241735.3 , XM_011241735.2
Accession Version	XM_011241735.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2532bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	remodeling and spacing factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011241735.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGCGGCGGC GGCGGCGGCG GCAGCGGCAG CGGCGATGGC TCCTCCGGGC 
TGCCCGGGTT CGTGCCCCAA CTTCGCCGTA GTCTGCTCCT TCTTGGAACG CTACGGGCCG 
CTGCTGGACC TGCCCGAGTT GCCGTTCCCA GAGCTGGAGC GGGTGCTGCA GGCGCCGCCG 
CCAGACATCG GCAACGGAGA AGTACCAAAA GAATTGGTGG AGCTGCATTT GAAGTTAATG 
AGGAAGATTG GCAAATCCGT TACAGCAGAC AGATGGGAAA AATACTTGAT CAAGATCTGT 
CAAGAGTTTA ACAGTACCTG GGCATGGGAG ATGGAGAAGA AAGGCTATCT TGAAATGAGT 
GTTGAATGCA AACTAGCACT CTTAAAGTAT CTCTGTGAGT GTCAGTTTGA TGACAATCTT 
AAATTCAAGA ATATAATTAA TGAGGAGGAT GCTGATACTA TGCGTCTCCA GCCAATTGGG 
CGAGACAAAG ATGGCCTCAT GTATTGGTAC CAGTTGGATC AAGACCATAA TGTGAGAATG 
TACATAGAAG AACAAGATGA TCAAGATGGC TCTTCTTGGA AATGCATTGT CAGAAATCGA 
AACGAGTTGG CTGAGACTCT TGCACTCCTG AAAGCACAAA TTGATCCTGT CCTATTGAAA 
AACTCGAGCC AACAGGAAGA TTCTTCTCGG GAAAGCCCCA CCCTAGAGGA TGAGGAGACT 
AAGAAAGATG AAGAAACACC TAAACAAGAA GCATCAAAAG AAAATGAAAA GACAGGAGCT 
GATGAGCGGT CAGTGGATTC TGGGAAGTGT TCTGCAGCCA GCGCTCTAGA AGAACCAGCT 
GTGAAAACTG AGAAAGCAGA TGAAAAGGAG CTTGCGAAAC TGCCAGTCAT TGTAAAGCTA 
GAAAAGCCTT TGCCAGAAAC TGAGGAAAAA AAGATTATCA GAGAGGAAAG TGATTCTTTC 
AAGGAAAATG TCAAACCCAT TAAAGTAGAA GCAAAGGAAT GCAGAGCGGA CCCAAAAGAT 
CTCAAAGGCA GCTTGGAGCG GCTGGGCTCG CAGGAGCCTG AGCGAGCCGA CTTTGGTAGC 
AACATCAAAT CTCAAGACAT CATAGAGAAA TCTACTGAGG AAACAGAGAA GCTTAAAAAT 
GATCAGCAAG CCAAGATACC ACTGAAGAAA CGTGAAATTA AGCTGAGTGA TGATTTTGAC 
AGTCCAGTCA AAGGACCTTT GTGTAAGTCA GCTACTCCAA CTAAAGAAGT TTTGAAAGAT 
GACATAAAAC AAGAGGAAGA GACTTGTAAA CGGGTCTCTA CCATCAGTGC TTTGAGTCAT 
GAAGGGAAAC AACTTGTGAA TGGAGAGATT AATGATGACA AGGTAATTCC AAATTTTAAG 
ACAGAACAAA TGGAAATACA GCTTTGTGAT ACAAAAGAAG ATAGTGCTAG CATCCCTGCT 
AAGGATGGAA ATGCCTGCAT GGAGGGAAAT GGTACGGAGT GCTTAAATTC TGTCATAACC 
AGCACAAAGA CAAGTGAGCT TGAGAAAGAG GCCCCTCTAG GGAAGGGTAT AGACAGCTCT 
GTACCTGACA TAGAGAGCCT CAGTCAGAAA GCAAGGTTAG AGGAGCCTGG GCTTCTGAAC 
ATGGACATGC CTCTCGAACC TTCTGAGAAA GCCACGGATC TCTCTTTAAA ACCCACTTTG 
TCTGCCACAG AACCCTGCAG TACGAAAGTT GAAGAGAAGG CCCCCAGAAG TAAGAGTGAG 
AACCACACCC CAGGCATAGA ATGCCTGGAA AGGGTGGAGA AAGCCAAGAA GACCTCCATT 
GATAAGGACA TCCAAAAATT GAGTCCAATA CCAGAAGAAG TTGTAAGGGG TGCTCTAGAG 
TCAGAAAAGT CAGGTCCTTG TGAAGTGGCT GAAACTCCTC TCCCACTCTC GATGGCTGGG 
GCCAAGGAGA AACAGACCTC AGAAAAAAAG GATGTAGACT GTAGTAGAGG TTCGAGTGAA 
AGTCAGTCCC TTGAAAATGC AAGCCCAGAA ATTCTAAAAG AGGATTCTGA GTCCTCGAGG 
GAGGAAGTGG CCAAACTGGA CAATGCCCAG ACCTCTGGCA TGGAGGATAC TTCTGAAACA 
AAGGGCTCTG TGCAGAAAAA CAAATTTAAA TATAAGTTAG TTTCTGAAAG AAACAGTACT 
GCTTCAGAAA ACACTGAGAT CACCTCTGAA AGGAAGAAGG AAGGCATCAA GTTGACAATT 
AGGATATCAA GTCGGAAGAA GAAGCCAGAT TGCCCCCCAC AAACTGTAGA CTCAGAAAGC 
AAGGAAGAGA AGGCGGGCAA GGAAGAAGAG AAAGCAAGTG TGGGTCGAAC TTTAAGAAGG 
TCTCCAAGAA TATCTAGACC CACAGCAAAA GTCGCTGAGA TCAGAGTCCA GAAAGCCGAT 
AAGAGAAGAG CTGACGGAGA AGATGGAGTG GAAGGGGAGC CGGCATCTTT GCAAACAGCA 
GACAAGAAGG ATCACTTGAA GAAAGCAGAG AAGGATACAA ATTCTAAAGC AAGCAAGGCA 
ATGCTGCATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011240037.1
CDS	35..2566
Translation	MAAAAAAAAAAAAAAMAPPGCPGSCPNFAVVCSFLERYGPLLDLPELPFPELERVLQAPPPDIGNGEVPKELVELHLKLMRKIGKSVTADRWEKYLIKICQEFNSTWAWEMEKKGYLEMSVECKLALLKYLCECQFDDNLKFKNIINEEDADTMRLQPIGRDKDGLMYWYQLDQDHNVRMYIEEQDDQDGSSWKCIVRNRNELAETLALLKAQIDPVLLKNSSQQEDSSRESPTLEDEETKKDEETPKQEASKENEKTGADERSVDSGKCSAASALEEPAVKTEKADEKELAKLPVIVKLEKPLPETEEKKIIREESDSFKENVKPIKVEAKECRADPKDLKGSLERLGSQEPERADFGSNIKSQDIIEKSTEETEKLKNDQQAKIPLKKREIKLSDDFDSPVKGPLCKSATPTKEVLKDDIKQEEETCKRVSTISALSHEGKQLVNGEINDDKVIPNFKTEQMEIQLCDTKEDSASIPAKDGNACMEGNGTECLNSVITSTKTSELEKEAPLGKGIDSSVPDIESLSQKARLEEPGLLNMDMPLEPSEKATDLSLKPTLSATEPCSTKVEEKAPRSKSENHTPGIECLERVEKAKKTSIDKDIQKLSPIPEEVVRGALESEKSGPCEVAETPLPLSMAGAKEKQTSEKKDVDCSRGSSESQSLENASPEILKEDSESSREEVAKLDNAQTSGMEDTSETKGSVQKNKFKYKLVSERNSTASENTEITSERKKEGIKLTIRISSRKKKPDCPPQTVDSESKEEKAGKEEEKASVGRTLRRSPRISRPTAKVAEIRVQKADKRRADGEDGVEGEPASLQTADKKDHLKKAEKDTNSKASKAMLH

Target ORF information:

RefSeq Version	XM_011241735.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus remodeling and spacing factor 1 (Rsf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011241735.3

ORF Insert Sequence:

ATGGCGGCGG CGGCGGCGGC GGCGGCGGCG GCAGCGGCAG CGGCGATGGC TCCTCCGGGC 
TGCCCGGGTT CGTGCCCCAA CTTCGCCGTA GTCTGCTCCT TCTTGGAACG CTACGGGCCG 
CTGCTGGACC TGCCCGAGTT GCCGTTCCCA GAGCTGGAGC GGGTGCTGCA GGCGCCGCCG 
CCAGACATCG GCAACGGAGA AGTACCAAAA GAATTGGTGG AGCTGCATTT GAAGTTAATG 
AGGAAGATTG GCAAATCCGT TACAGCAGAC AGATGGGAAA AATACTTGAT CAAGATCTGT 
CAAGAGTTTA ACAGTACCTG GGCATGGGAG ATGGAGAAGA AAGGCTATCT TGAAATGAGT 
GTTGAATGCA AACTAGCACT CTTAAAGTAT CTCTGTGAGT GTCAGTTTGA TGACAATCTT 
AAATTCAAGA ATATAATTAA TGAGGAGGAT GCTGATACTA TGCGTCTCCA GCCAATTGGG 
CGAGACAAAG ATGGCCTCAT GTATTGGTAC CAGTTGGATC AAGACCATAA TGTGAGAATG 
TACATAGAAG AACAAGATGA TCAAGATGGC TCTTCTTGGA AATGCATTGT CAGAAATCGA 
AACGAGTTGG CTGAGACTCT TGCACTCCTG AAAGCACAAA TTGATCCTGT CCTATTGAAA 
AACTCGAGCC AACAGGAAGA TTCTTCTCGG GAAAGCCCCA CCCTAGAGGA TGAGGAGACT 
AAGAAAGATG AAGAAACACC TAAACAAGAA GCATCAAAAG AAAATGAAAA GACAGGAGCT 
GATGAGCGGT CAGTGGATTC TGGGAAGTGT TCTGCAGCCA GCGCTCTAGA AGAACCAGCT 
GTGAAAACTG AGAAAGCAGA TGAAAAGGAG CTTGCGAAAC TGCCAGTCAT TGTAAAGCTA 
GAAAAGCCTT TGCCAGAAAC TGAGGAAAAA AAGATTATCA GAGAGGAAAG TGATTCTTTC 
AAGGAAAATG TCAAACCCAT TAAAGTAGAA GCAAAGGAAT GCAGAGCGGA CCCAAAAGAT 
CTCAAAGGCA GCTTGGAGCG GCTGGGCTCG CAGGAGCCTG AGCGAGCCGA CTTTGGTAGC 
AACATCAAAT CTCAAGACAT CATAGAGAAA TCTACTGAGG AAACAGAGAA GCTTAAAAAT 
GATCAGCAAG CCAAGATACC ACTGAAGAAA CGTGAAATTA AGCTGAGTGA TGATTTTGAC 
AGTCCAGTCA AAGGACCTTT GTGTAAGTCA GCTACTCCAA CTAAAGAAGT TTTGAAAGAT 
GACATAAAAC AAGAGGAAGA GACTTGTAAA CGGGTCTCTA CCATCAGTGC TTTGAGTCAT 
GAAGGGAAAC AACTTGTGAA TGGAGAGATT AATGATGACA AGGTAATTCC AAATTTTAAG 
ACAGAACAAA TGGAAATACA GCTTTGTGAT ACAAAAGAAG ATAGTGCTAG CATCCCTGCT 
AAGGATGGAA ATGCCTGCAT GGAGGGAAAT GGTACGGAGT GCTTAAATTC TGTCATAACC 
AGCACAAAGA CAAGTGAGCT TGAGAAAGAG GCCCCTCTAG GGAAGGGTAT AGACAGCTCT 
GTACCTGACA TAGAGAGCCT CAGTCAGAAA GCAAGGTTAG AGGAGCCTGG GCTTCTGAAC 
ATGGACATGC CTCTCGAACC TTCTGAGAAA GCCACGGATC TCTCTTTAAA ACCCACTTTG 
TCTGCCACAG AACCCTGCAG TACGAAAGTT GAAGAGAAGG CCCCCAGAAG TAAGAGTGAG 
AACCACACCC CAGGCATAGA ATGCCTGGAA AGGGTGGAGA AAGCCAAGAA GACCTCCATT 
GATAAGGACA TCCAAAAATT GAGTCCAATA CCAGAAGAAG TTGTAAGGGG TGCTCTAGAG 
TCAGAAAAGT CAGGTCCTTG TGAAGTGGCT GAAACTCCTC TCCCACTCTC GATGGCTGGG 
GCCAAGGAGA AACAGACCTC AGAAAAAAAG GATGTAGACT GTAGTAGAGG TTCGAGTGAA 
AGTCAGTCCC TTGAAAATGC AAGCCCAGAA ATTCTAAAAG AGGATTCTGA GTCCTCGAGG 
GAGGAAGTGG CCAAACTGGA CAATGCCCAG ACCTCTGGCA TGGAGGATAC TTCTGAAACA 
AAGGGCTCTG TGCAGAAAAA CAAATTTAAA TATAAGTTAG TTTCTGAAAG AAACAGTACT 
GCTTCAGAAA ACACTGAGAT CACCTCTGAA AGGAAGAAGG AAGGCATCAA GTTGACAATT 
AGGATATCAA GTCGGAAGAA GAAGCCAGAT TGCCCCCCAC AAACTGTAGA CTCAGAAAGC 
AAGGAAGAGA AGGCGGGCAA GGAAGAAGAG AAAGCAAGTG TGGGTCGAAC TTTAAGAAGG 
TCTCCAAGAA TATCTAGACC CACAGCAAAA GTCGCTGAGA TCAGAGTCCA GAAAGCCGAT 
AAGAGAAGAG CTGACGGAGA AGATGGAGTG GAAGGGGAGC CGGCATCTTT GCAAACAGCA 
GACAAGAAGG ATCACTTGAA GAAAGCAGAG AAGGATACAA ATTCTAAAGC AAGCAAGGCA 
ATGCTGCATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rsf1 ( NM_001081267.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001074736.1 Mus musculus remodeling and spacing factor 1 (Rsf1), mRNA.

Price & Availability↑

CloneID	OMu01993
Clone ID Related Accession (Same CDS sequence)	NM_001081267.2
Accession Version	NM_001081267.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4326bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-25
Organism	Mus musculus(house mouse)
Product	remodeling and spacing factor 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC149605.7, AC124541.4 and AC151477.1. On Dec 2, 2009 this sequence version replaced NM_001081267.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849382, SAMN00849384 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGG CGGCGGCGGC GGCGGCGGCG GCAGCGGCAG CGGCGATGGC TCCTCCGGGC 
TGCCCGGGTT CGTGCCCCAA CTTCGCCGTA GTCTGCTCCT TCTTGGAACG CTACGGGCCG 
CTGCTGGACC TGCCCGAGTT GCCGTTCCCA GAGCTGGAGC GGGTGCTGCA GGCGCCGCCG 
CCAGACATCG GCAACGGAGA AGTACCAAAA GAATTGGTGG AGCTGCATTT GAAGTTAATG 
AGGAAGATTG GCAAATCCGT TACAGCAGAC AGATGGGAAA AATACTTGAT CAAGATCTGT 
CAAGAGTTTA ACAGTACCTG GGCATGGGAG ATGGAGAAGA AAGGCTATCT TGAAATGAGT 
GTTGAATGCA AACTAGCACT CTTAAAGTAT CTCTGTGAGT GTCAGTTTGA TGACAATCTT 
AAATTCAAGA ATATAATTAA TGAGGAGGAT GCTGATACTA TGCGTCTCCA GCCAATTGGG 
CGAGACAAAG ATGGCCTCAT GTATTGGTAC CAGTTGGATC AAGACCATAA TGTGAGAATG 
TACATAGAAG AACAAGATGA TCAAGATGGC TCTTCTTGGA AATGCATTGT CAGAAATCGA 
AACGAGTTGG CTGAGACTCT TGCACTCCTG AAAGCACAAA TTGATCCTGT CCTATTGAAA 
AACTCGAGCC AACAGGAAGA TTCTTCTCGG GAAAGCCCCA CCCTAGAGGA TGAGGAGACT 
AAGAAAGATG AAGAAACACC TAAACAAGAA GCATCAAAAG AAAATGAAAA GACAGGAGCT 
GATGAGCGGT CAGTGGATTC TGGGAAGTGT TCTGCAGCCA GCGCTCTAGA AGAACCAGCT 
GTGAAAACTG AGAAAGCAGA TGAAAAGGAG CTTGCGAAAC TGCCAGTCAT TGTAAAGCTA 
GAAAAGCCTT TGCCAGAAAC TGAGGAAAAA AAGATTATCA GAGAGGAAAG TGATTCTTTC 
AAGGAAAATG TCAAACCCAT TAAAGTAGAA GCAAAGGAAT GCAGAGCGGA CCCAAAAGAT 
CTCAAAGGCA GCTTGGAGCG GCTGGGCTCG CAGGAGCCTG AGCGAGCCGA CTTTGGTAGC 
AACATCAAAT CTCAAGACAT CATAGAGAAA TCTACTGAGG AAACAGAGAA GCTTAAAAAT 
GATCAGCAAG CCAAGATACC ACTGAAGAAA CGTGAAATTA AGCTGAGTGA TGATTTTGAC 
AGTCCAGTCA AAGGACCTTT GTGTAAGTCA GCTACTCCAA CTAAAGAAGT TTTGAAAGAT 
GACATAAAAC AAGAGGAAGA GACTTGTAAA CGGGTCTCTA CCATCAGTGC TTTGAGTCAT 
GAAGGGAAAC AACTTGTGAA TGGAGAGATT AATGATGACA AGGTAATTCC AAATTTTAAG 
ACAGAACAAA TGGAAATACA GCTTTGTGAT ACAAAAGAAG ATAGTGCTAG CATCCCTGCT 
AAGGATGGAA ATGCCTGCAT GGAGGGAAAT GGTACGGAGT GCTTAAATTC TGTCATAACC 
AGCACAAAGA CAAGTGAGCT TGAGAAAGAG GCCCCTCTAG GGAAGGGTAT AGACAGCTCT 
GTACCTGACA TAGAGAGCCT CAGTCAGAAA GCAAGGTTAG AGGAGCCTGG GCTTCTGAAC 
ATGGACATGC CTCTCGAACC TTCTGAGAAA GCCACGGATC TCTCTTTAAA ACCCACTTTG 
TCTGCCACAG AACCCTGCAG TACGAAAGTT GAAGAGAAGG CCCCCAGAAG TAAGAGTGAG 
AACCACACCC CAGGCATAGA ATGCCTGGAA AGGGTGGAGA AAGCCAAGAA GACCTCCATT 
GATAAGGACA TCCAAAAATT GAGTCCAATA CCAGAAGAAG TTGTAAGGGG TGCTCTAGAG 
TCAGAAAAGT CAGGTCCTTG TGAAGTGGCT GAAACTCCTC TCCCACTCTC GATGGCTGGG 
GCCAAGGAGA AACAGACCTC AGAAAAAAAG GATGTAGACT GTAGTAGAGG TTCGAGTGAA 
AGTCAGTCCC TTGAAAATGC AAGCCCAGAA ATTCTAAAAG AGGATTCTGA GTCCTCGAGG 
GAGGAAGTGG CCAAACTGGA CAATGCCCAG ACCTCTGGCA TGGAGGATAC TTCTGAAACA 
AAGGGCTCTG TGCAGAAAAA CAAATTTAAA TATAAGTTAG TTTCTGAAAG AAACAGTACT 
GCTTCAGAAA ACACTGAGAT CACCTCTGAA AGGAAGAAGG AAGGCATCAA GTTGACAATT 
AGGATATCAA GTCGGAAGAA GAAGCCAGAT TGCCCCCCAC AAACTGTAGA CTCAGAAAGC 
AAGGAAGAGA AGGCGGGCAA GGAAGAAGAG AAAGCAAGTG TGGGTCGAAC TTTAAGAAGG 
TCTCCAAGAA TATCTAGACC CACAGCAAAA GTCGCTGAGA TCAGAGTCCA GAAAGCCGAT 
AAGAGAAGAG CTGACGGAGA AGATGGAGTG GAAGGGGAGC CGGCATCTTT GCAAACAGCA 
GACAAGAAGG ATCACTTGAA GAAAGCAGAG AAGGATACAA ATTCTAAAGC AAGCAAGGTA 
AAACCCAAAG GCAAAGTTCG ATGGACTGGC TCTCGGACAC GAGGCAGGTG GAAATACTCC 
AGCAATGACG AAAGTGAAGG ATCCGAGAGT GACAAATCCT CTGCAGCCTT GGAAGAGGAG 
GAAGGAAAGG AAGGTGAAGA AGCGGTCCTT CCAGATGACG ATGAACCTTG CAAAAAGTGT 
GGCCTTCCGA ACCATCCAGA ACTAATTCTT CTGTGTGATT CTTGCGACAG TGGATACCAT 
ACTGCCTGCC TCCGCCCTCC GCTGATGATT ATCCCTGATG GAGAATGGTT CTGCCCTCCT 
TGCCAACATA AACTACTGTG TGAAAAGTTA GAAGAACAGC TGCAAGATTT GGACGTCGCC 
TTAAAGAAGA AAGAGCGTGC AGAGCGAAGG AAAGAACGTT TGGTGTATGT TGGTATCAGT 
ATTGAAAACA TCATTCCTCC ACAAGAGCCT GAGTTTTCTG AAGAGCAAGA AGAAAAGAAA 
AAAGATGCAA AAAAATCCAA AGCAAATGCA CTTGAAAGGA GATCAACAAG AACAAGGAAA 
TGTATTAGCT ACAGATTTGA TGAATTTGAT GAAGCAATTG ATGAAGCTAT AGAAGATGAT 
ATTAAAGAGG CTGATGGAGG AGGAGTTGGC CGGGGGAAAG ATATCTCCAC CATCACAGGT 
CACCGTGGGA AAGACATTTC TACTATTTTG GATGAAGAAA GAAAGGAAAA TAAACGGCCC 
CAGAGGGCAG CTGCTGCTCG CAGGAAGAAA CGCCGGCGAC TTAATGATCT TGACAGTGAT 
AGCAACCTAG ATGAAGAAGA GAGTGAGGAT GAATTCAAGA TCAGTGACGG ATCTCAGGAT 
GAGTTTGTTG TGTCTGATGA AAATCCAGAT GAAAGTGAAG AAGAGCCACC ATCCAATGAG 
GACAGCGACA CTGACTTCTG CAGCCGAAGA CTGAGGCGGC ATCCTTCTCG GCCAATGAGG 
CAAAGCAGGC GTTTGCGGAG AAAGACCCCA AAGAAGAAGT ACTCCGATGA CGATGAGGAG 
GAGGAAGAAT CCGAGGAGAA CAGCAGAGAC TCTGAAAGTG ACTTTAGCGA TGATTTTAGT 
GATGATTTTG TAGAAACCCG GCGAAGGCGA TCAAGAAGGA ACCAGAAAAG ACAAATTAAC 
TATAAAGAAG ATTCAGAAAG TGATGGTTCC CAGAAAAGTC TACGACGTGG TAAAGAAATC 
AGGCGAGTTC ACAAGCGCAG ACTTTCCAGC TCAGAGAGTG AAGAGAGCTT TATGTCCAAG 
AACTCTGAAG ATGATGAGCT AACAAAAGAA TCGAAGCGAT CTGTTCGAAA ACGGGGCCGA 
AGCACAGATG ACTATTCAGA AGCAGATGAG GATGATGAAG AGGAAGGCAA ACCATCCCGC 
AAGCGGCTAC ACCGGATTGA GACGGACGAG GAGAGCTGTG ACAATGCTCA TGGGGACGCA 
GACCAGCCTG CCCGTGACAG CCAGCCCAGT GCCCTGCCCT CAGAGCAGGA GAGCAGCAAG 
AAGCCCTACC GGATAGAGAG TGATGAGGAA GAAGACTTTG AAAACAAAGT AGGGAGCCCA 
TTGGACTACA GCCTAGTAGA CTTGCCTTCC ACCAATGGAC AGAGCCCTGG CAAGGCCATT 
GAGAACTTGA TGGGCAAAGC AACTGAAAAG TCCCAAACCC CCAAGGACAA TGGCACAGCC 
AGTGCAAGTC TAGCCCCCAA TGGGACAAGT GGTGGACAGG AGGTGGGGGC ACCAGAGGAA 
GATGAAGATG AGCTTTTGCG AGTGACTGAT CTTGTTGATT ACGTCTGTAA CAGTGAACAG 
TTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074736.1
CDS	9..4334
Translation	MAAAAAAAAAAAAAAMAPPGCPGSCPNFAVVCSFLERYGPLLDLPELPFPELERVLQAPPPDIGNGEVPKELVELHLKLMRKIGKSVTADRWEKYLIKICQEFNSTWAWEMEKKGYLEMSVECKLALLKYLCECQFDDNLKFKNIINEEDADTMRLQPIGRDKDGLMYWYQLDQDHNVRMYIEEQDDQDGSSWKCIVRNRNELAETLALLKAQIDPVLLKNSSQQEDSSRESPTLEDEETKKDEETPKQEASKENEKTGADERSVDSGKCSAASALEEPAVKTEKADEKELAKLPVIVKLEKPLPETEEKKIIREESDSFKENVKPIKVEAKECRADPKDLKGSLERLGSQEPERADFGSNIKSQDIIEKSTEETEKLKNDQQAKIPLKKREIKLSDDFDSPVKGPLCKSATPTKEVLKDDIKQEEETCKRVSTISALSHEGKQLVNGEINDDKVIPNFKTEQMEIQLCDTKEDSASIPAKDGNACMEGNGTECLNSVITSTKTSELEKEAPLGKGIDSSVPDIESLSQKARLEEPGLLNMDMPLEPSEKATDLSLKPTLSATEPCSTKVEEKAPRSKSENHTPGIECLERVEKAKKTSIDKDIQKLSPIPEEVVRGALESEKSGPCEVAETPLPLSMAGAKEKQTSEKKDVDCSRGSSESQSLENASPEILKEDSESSREEVAKLDNAQTSGMEDTSETKGSVQKNKFKYKLVSERNSTASENTEITSERKKEGIKLTIRISSRKKKPDCPPQTVDSESKEEKAGKEEEKASVGRTLRRSPRISRPTAKVAEIRVQKADKRRADGEDGVEGEPASLQTADKKDHLKKAEKDTNSKASKVKPKGKVRWTGSRTRGRWKYSSNDESEGSESDKSSAALEEEEGKEGEEAVLPDDDEPCKKCGLPNHPELILLCDSCDSGYHTACLRPPLMIIPDGEWFCPPCQHKLLCEKLEEQLQDLDVALKKKERAERRKERLVYVGISIENIIPPQEPEFSEEQEEKKKDAKKSKANALERRSTRTRKCISYRFDEFDEAIDEAIEDDIKEADGGGVGRGKDISTITGHRGKDISTILDEERKENKRPQRAAAARRKKRRRLNDLDSDSNLDEEESEDEFKISDGSQDEFVVSDENPDESEEEPPSNEDSDTDFCSRRLRRHPSRPMRQSRRLRRKTPKKKYSDDDEEEEESEENSRDSESDFSDDFSDDFVETRRRRSRRNQKRQINYKEDSESDGSQKSLRRGKEIRRVHKRRLSSSESEESFMSKNSEDDELTKESKRSVRKRGRSTDDYSEADEDDEEEGKPSRKRLHRIETDEESCDNAHGDADQPARDSQPSALPSEQESSKKPYRIESDEEEDFENKVGSPLDYSLVDLPSTNGQSPGKAIENLMGKATEKSQTPKDNGTASASLAPNGTSGGQEVGAPEEDEDELLRVTDLVDYVCNSEQL

Target ORF information:

RefSeq Version	NM_001081267.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus remodeling and spacing factor 1 (Rsf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081267.2

ORF Insert Sequence:

ATGGCGGCGG CGGCGGCGGC GGCGGCGGCG GCAGCGGCAG CGGCGATGGC TCCTCCGGGC 
TGCCCGGGTT CGTGCCCCAA CTTCGCCGTA GTCTGCTCCT TCTTGGAACG CTACGGGCCG 
CTGCTGGACC TGCCCGAGTT GCCGTTCCCA GAGCTGGAGC GGGTGCTGCA GGCGCCGCCG 
CCAGACATCG GCAACGGAGA AGTACCAAAA GAATTGGTGG AGCTGCATTT GAAGTTAATG 
AGGAAGATTG GCAAATCCGT TACAGCAGAC AGATGGGAAA AATACTTGAT CAAGATCTGT 
CAAGAGTTTA ACAGTACCTG GGCATGGGAG ATGGAGAAGA AAGGCTATCT TGAAATGAGT 
GTTGAATGCA AACTAGCACT CTTAAAGTAT CTCTGTGAGT GTCAGTTTGA TGACAATCTT 
AAATTCAAGA ATATAATTAA TGAGGAGGAT GCTGATACTA TGCGTCTCCA GCCAATTGGG 
CGAGACAAAG ATGGCCTCAT GTATTGGTAC CAGTTGGATC AAGACCATAA TGTGAGAATG 
TACATAGAAG AACAAGATGA TCAAGATGGC TCTTCTTGGA AATGCATTGT CAGAAATCGA 
AACGAGTTGG CTGAGACTCT TGCACTCCTG AAAGCACAAA TTGATCCTGT CCTATTGAAA 
AACTCGAGCC AACAGGAAGA TTCTTCTCGG GAAAGCCCCA CCCTAGAGGA TGAGGAGACT 
AAGAAAGATG AAGAAACACC TAAACAAGAA GCATCAAAAG AAAATGAAAA GACAGGAGCT 
GATGAGCGGT CAGTGGATTC TGGGAAGTGT TCTGCAGCCA GCGCTCTAGA AGAACCAGCT 
GTGAAAACTG AGAAAGCAGA TGAAAAGGAG CTTGCGAAAC TGCCAGTCAT TGTAAAGCTA 
GAAAAGCCTT TGCCAGAAAC TGAGGAAAAA AAGATTATCA GAGAGGAAAG TGATTCTTTC 
AAGGAAAATG TCAAACCCAT TAAAGTAGAA GCAAAGGAAT GCAGAGCGGA CCCAAAAGAT 
CTCAAAGGCA GCTTGGAGCG GCTGGGCTCG CAGGAGCCTG AGCGAGCCGA CTTTGGTAGC 
AACATCAAAT CTCAAGACAT CATAGAGAAA TCTACTGAGG AAACAGAGAA GCTTAAAAAT 
GATCAGCAAG CCAAGATACC ACTGAAGAAA CGTGAAATTA AGCTGAGTGA TGATTTTGAC 
AGTCCAGTCA AAGGACCTTT GTGTAAGTCA GCTACTCCAA CTAAAGAAGT TTTGAAAGAT 
GACATAAAAC AAGAGGAAGA GACTTGTAAA CGGGTCTCTA CCATCAGTGC TTTGAGTCAT 
GAAGGGAAAC AACTTGTGAA TGGAGAGATT AATGATGACA AGGTAATTCC AAATTTTAAG 
ACAGAACAAA TGGAAATACA GCTTTGTGAT ACAAAAGAAG ATAGTGCTAG CATCCCTGCT 
AAGGATGGAA ATGCCTGCAT GGAGGGAAAT GGTACGGAGT GCTTAAATTC TGTCATAACC 
AGCACAAAGA CAAGTGAGCT TGAGAAAGAG GCCCCTCTAG GGAAGGGTAT AGACAGCTCT 
GTACCTGACA TAGAGAGCCT CAGTCAGAAA GCAAGGTTAG AGGAGCCTGG GCTTCTGAAC 
ATGGACATGC CTCTCGAACC TTCTGAGAAA GCCACGGATC TCTCTTTAAA ACCCACTTTG 
TCTGCCACAG AACCCTGCAG TACGAAAGTT GAAGAGAAGG CCCCCAGAAG TAAGAGTGAG 
AACCACACCC CAGGCATAGA ATGCCTGGAA AGGGTGGAGA AAGCCAAGAA GACCTCCATT 
GATAAGGACA TCCAAAAATT GAGTCCAATA CCAGAAGAAG TTGTAAGGGG TGCTCTAGAG 
TCAGAAAAGT CAGGTCCTTG TGAAGTGGCT GAAACTCCTC TCCCACTCTC GATGGCTGGG 
GCCAAGGAGA AACAGACCTC AGAAAAAAAG GATGTAGACT GTAGTAGAGG TTCGAGTGAA 
AGTCAGTCCC TTGAAAATGC AAGCCCAGAA ATTCTAAAAG AGGATTCTGA GTCCTCGAGG 
GAGGAAGTGG CCAAACTGGA CAATGCCCAG ACCTCTGGCA TGGAGGATAC TTCTGAAACA 
AAGGGCTCTG TGCAGAAAAA CAAATTTAAA TATAAGTTAG TTTCTGAAAG AAACAGTACT 
GCTTCAGAAA ACACTGAGAT CACCTCTGAA AGGAAGAAGG AAGGCATCAA GTTGACAATT 
AGGATATCAA GTCGGAAGAA GAAGCCAGAT TGCCCCCCAC AAACTGTAGA CTCAGAAAGC 
AAGGAAGAGA AGGCGGGCAA GGAAGAAGAG AAAGCAAGTG TGGGTCGAAC TTTAAGAAGG 
TCTCCAAGAA TATCTAGACC CACAGCAAAA GTCGCTGAGA TCAGAGTCCA GAAAGCCGAT 
AAGAGAAGAG CTGACGGAGA AGATGGAGTG GAAGGGGAGC CGGCATCTTT GCAAACAGCA 
GACAAGAAGG ATCACTTGAA GAAAGCAGAG AAGGATACAA ATTCTAAAGC AAGCAAGGTA 
AAACCCAAAG GCAAAGTTCG ATGGACTGGC TCTCGGACAC GAGGCAGGTG GAAATACTCC 
AGCAATGACG AAAGTGAAGG ATCCGAGAGT GACAAATCCT CTGCAGCCTT GGAAGAGGAG 
GAAGGAAAGG AAGGTGAAGA AGCGGTCCTT CCAGATGACG ATGAACCTTG CAAAAAGTGT 
GGCCTTCCGA ACCATCCAGA ACTAATTCTT CTGTGTGATT CTTGCGACAG TGGATACCAT 
ACTGCCTGCC TCCGCCCTCC GCTGATGATT ATCCCTGATG GAGAATGGTT CTGCCCTCCT 
TGCCAACATA AACTACTGTG TGAAAAGTTA GAAGAACAGC TGCAAGATTT GGACGTCGCC 
TTAAAGAAGA AAGAGCGTGC AGAGCGAAGG AAAGAACGTT TGGTGTATGT TGGTATCAGT 
ATTGAAAACA TCATTCCTCC ACAAGAGCCT GAGTTTTCTG AAGAGCAAGA AGAAAAGAAA 
AAAGATGCAA AAAAATCCAA AGCAAATGCA CTTGAAAGGA GATCAACAAG AACAAGGAAA 
TGTATTAGCT ACAGATTTGA TGAATTTGAT GAAGCAATTG ATGAAGCTAT AGAAGATGAT 
ATTAAAGAGG CTGATGGAGG AGGAGTTGGC CGGGGGAAAG ATATCTCCAC CATCACAGGT 
CACCGTGGGA AAGACATTTC TACTATTTTG GATGAAGAAA GAAAGGAAAA TAAACGGCCC 
CAGAGGGCAG CTGCTGCTCG CAGGAAGAAA CGCCGGCGAC TTAATGATCT TGACAGTGAT 
AGCAACCTAG ATGAAGAAGA GAGTGAGGAT GAATTCAAGA TCAGTGACGG ATCTCAGGAT 
GAGTTTGTTG TGTCTGATGA AAATCCAGAT GAAAGTGAAG AAGAGCCACC ATCCAATGAG 
GACAGCGACA CTGACTTCTG CAGCCGAAGA CTGAGGCGGC ATCCTTCTCG GCCAATGAGG 
CAAAGCAGGC GTTTGCGGAG AAAGACCCCA AAGAAGAAGT ACTCCGATGA CGATGAGGAG 
GAGGAAGAAT CCGAGGAGAA CAGCAGAGAC TCTGAAAGTG ACTTTAGCGA TGATTTTAGT 
GATGATTTTG TAGAAACCCG GCGAAGGCGA TCAAGAAGGA ACCAGAAAAG ACAAATTAAC 
TATAAAGAAG ATTCAGAAAG TGATGGTTCC CAGAAAAGTC TACGACGTGG TAAAGAAATC 
AGGCGAGTTC ACAAGCGCAG ACTTTCCAGC TCAGAGAGTG AAGAGAGCTT TATGTCCAAG 
AACTCTGAAG ATGATGAGCT AACAAAAGAA TCGAAGCGAT CTGTTCGAAA ACGGGGCCGA 
AGCACAGATG ACTATTCAGA AGCAGATGAG GATGATGAAG AGGAAGGCAA ACCATCCCGC 
AAGCGGCTAC ACCGGATTGA GACGGACGAG GAGAGCTGTG ACAATGCTCA TGGGGACGCA 
GACCAGCCTG CCCGTGACAG CCAGCCCAGT GCCCTGCCCT CAGAGCAGGA GAGCAGCAAG 
AAGCCCTACC GGATAGAGAG TGATGAGGAA GAAGACTTTG AAAACAAAGT AGGGAGCCCA 
TTGGACTACA GCCTAGTAGA CTTGCCTTCC ACCAATGGAC AGAGCCCTGG CAAGGCCATT 
GAGAACTTGA TGGGCAAAGC AACTGAAAAG TCCCAAACCC CCAAGGACAA TGGCACAGCC 
AGTGCAAGTC TAGCCCCCAA TGGGACAAGT GGTGGACAGG AGGTGGGGGC ACCAGAGGAA 
GATGAAGATG AGCTTTTGCG AGTGACTGAT CTTGTTGATT ACGTCTGTAA CAGTGAACAG 
TTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rsf1 ( XM_011241735.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011240037.1 Mus musculus remodeling and spacing factor 1 (Rsf1), transcript variant X1, mRNA.

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CloneID	OMu73683
Clone ID Related Accession (Same CDS sequence)	XM_011241735.3 , XM_011241735.2
Accession Version	XM_011241735.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2532bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	remodeling and spacing factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011241735.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGCGGCGGC GGCGGCGGCG GCAGCGGCAG CGGCGATGGC TCCTCCGGGC 
TGCCCGGGTT CGTGCCCCAA CTTCGCCGTA GTCTGCTCCT TCTTGGAACG CTACGGGCCG 
CTGCTGGACC TGCCCGAGTT GCCGTTCCCA GAGCTGGAGC GGGTGCTGCA GGCGCCGCCG 
CCAGACATCG GCAACGGAGA AGTACCAAAA GAATTGGTGG AGCTGCATTT GAAGTTAATG 
AGGAAGATTG GCAAATCCGT TACAGCAGAC AGATGGGAAA AATACTTGAT CAAGATCTGT 
CAAGAGTTTA ACAGTACCTG GGCATGGGAG ATGGAGAAGA AAGGCTATCT TGAAATGAGT 
GTTGAATGCA AACTAGCACT CTTAAAGTAT CTCTGTGAGT GTCAGTTTGA TGACAATCTT 
AAATTCAAGA ATATAATTAA TGAGGAGGAT GCTGATACTA TGCGTCTCCA GCCAATTGGG 
CGAGACAAAG ATGGCCTCAT GTATTGGTAC CAGTTGGATC AAGACCATAA TGTGAGAATG 
TACATAGAAG AACAAGATGA TCAAGATGGC TCTTCTTGGA AATGCATTGT CAGAAATCGA 
AACGAGTTGG CTGAGACTCT TGCACTCCTG AAAGCACAAA TTGATCCTGT CCTATTGAAA 
AACTCGAGCC AACAGGAAGA TTCTTCTCGG GAAAGCCCCA CCCTAGAGGA TGAGGAGACT 
AAGAAAGATG AAGAAACACC TAAACAAGAA GCATCAAAAG AAAATGAAAA GACAGGAGCT 
GATGAGCGGT CAGTGGATTC TGGGAAGTGT TCTGCAGCCA GCGCTCTAGA AGAACCAGCT 
GTGAAAACTG AGAAAGCAGA TGAAAAGGAG CTTGCGAAAC TGCCAGTCAT TGTAAAGCTA 
GAAAAGCCTT TGCCAGAAAC TGAGGAAAAA AAGATTATCA GAGAGGAAAG TGATTCTTTC 
AAGGAAAATG TCAAACCCAT TAAAGTAGAA GCAAAGGAAT GCAGAGCGGA CCCAAAAGAT 
CTCAAAGGCA GCTTGGAGCG GCTGGGCTCG CAGGAGCCTG AGCGAGCCGA CTTTGGTAGC 
AACATCAAAT CTCAAGACAT CATAGAGAAA TCTACTGAGG AAACAGAGAA GCTTAAAAAT 
GATCAGCAAG CCAAGATACC ACTGAAGAAA CGTGAAATTA AGCTGAGTGA TGATTTTGAC 
AGTCCAGTCA AAGGACCTTT GTGTAAGTCA GCTACTCCAA CTAAAGAAGT TTTGAAAGAT 
GACATAAAAC AAGAGGAAGA GACTTGTAAA CGGGTCTCTA CCATCAGTGC TTTGAGTCAT 
GAAGGGAAAC AACTTGTGAA TGGAGAGATT AATGATGACA AGGTAATTCC AAATTTTAAG 
ACAGAACAAA TGGAAATACA GCTTTGTGAT ACAAAAGAAG ATAGTGCTAG CATCCCTGCT 
AAGGATGGAA ATGCCTGCAT GGAGGGAAAT GGTACGGAGT GCTTAAATTC TGTCATAACC 
AGCACAAAGA CAAGTGAGCT TGAGAAAGAG GCCCCTCTAG GGAAGGGTAT AGACAGCTCT 
GTACCTGACA TAGAGAGCCT CAGTCAGAAA GCAAGGTTAG AGGAGCCTGG GCTTCTGAAC 
ATGGACATGC CTCTCGAACC TTCTGAGAAA GCCACGGATC TCTCTTTAAA ACCCACTTTG 
TCTGCCACAG AACCCTGCAG TACGAAAGTT GAAGAGAAGG CCCCCAGAAG TAAGAGTGAG 
AACCACACCC CAGGCATAGA ATGCCTGGAA AGGGTGGAGA AAGCCAAGAA GACCTCCATT 
GATAAGGACA TCCAAAAATT GAGTCCAATA CCAGAAGAAG TTGTAAGGGG TGCTCTAGAG 
TCAGAAAAGT CAGGTCCTTG TGAAGTGGCT GAAACTCCTC TCCCACTCTC GATGGCTGGG 
GCCAAGGAGA AACAGACCTC AGAAAAAAAG GATGTAGACT GTAGTAGAGG TTCGAGTGAA 
AGTCAGTCCC TTGAAAATGC AAGCCCAGAA ATTCTAAAAG AGGATTCTGA GTCCTCGAGG 
GAGGAAGTGG CCAAACTGGA CAATGCCCAG ACCTCTGGCA TGGAGGATAC TTCTGAAACA 
AAGGGCTCTG TGCAGAAAAA CAAATTTAAA TATAAGTTAG TTTCTGAAAG AAACAGTACT 
GCTTCAGAAA ACACTGAGAT CACCTCTGAA AGGAAGAAGG AAGGCATCAA GTTGACAATT 
AGGATATCAA GTCGGAAGAA GAAGCCAGAT TGCCCCCCAC AAACTGTAGA CTCAGAAAGC 
AAGGAAGAGA AGGCGGGCAA GGAAGAAGAG AAAGCAAGTG TGGGTCGAAC TTTAAGAAGG 
TCTCCAAGAA TATCTAGACC CACAGCAAAA GTCGCTGAGA TCAGAGTCCA GAAAGCCGAT 
AAGAGAAGAG CTGACGGAGA AGATGGAGTG GAAGGGGAGC CGGCATCTTT GCAAACAGCA 
GACAAGAAGG ATCACTTGAA GAAAGCAGAG AAGGATACAA ATTCTAAAGC AAGCAAGGCA 
ATGCTGCATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011240037.1
CDS	139..2670
Translation	MAAAAAAAAAAAAAAMAPPGCPGSCPNFAVVCSFLERYGPLLDLPELPFPELERVLQAPPPDIGNGEVPKELVELHLKLMRKIGKSVTADRWEKYLIKICQEFNSTWAWEMEKKGYLEMSVECKLALLKYLCECQFDDNLKFKNIINEEDADTMRLQPIGRDKDGLMYWYQLDQDHNVRMYIEEQDDQDGSSWKCIVRNRNELAETLALLKAQIDPVLLKNSSQQEDSSRESPTLEDEETKKDEETPKQEASKENEKTGADERSVDSGKCSAASALEEPAVKTEKADEKELAKLPVIVKLEKPLPETEEKKIIREESDSFKENVKPIKVEAKECRADPKDLKGSLERLGSQEPERADFGSNIKSQDIIEKSTEETEKLKNDQQAKIPLKKREIKLSDDFDSPVKGPLCKSATPTKEVLKDDIKQEEETCKRVSTISALSHEGKQLVNGEINDDKVIPNFKTEQMEIQLCDTKEDSASIPAKDGNACMEGNGTECLNSVITSTKTSELEKEAPLGKGIDSSVPDIESLSQKARLEEPGLLNMDMPLEPSEKATDLSLKPTLSATEPCSTKVEEKAPRSKSENHTPGIECLERVEKAKKTSIDKDIQKLSPIPEEVVRGALESEKSGPCEVAETPLPLSMAGAKEKQTSEKKDVDCSRGSSESQSLENASPEILKEDSESSREEVAKLDNAQTSGMEDTSETKGSVQKNKFKYKLVSERNSTASENTEITSERKKEGIKLTIRISSRKKKPDCPPQTVDSESKEEKAGKEEEKASVGRTLRRSPRISRPTAKVAEIRVQKADKRRADGEDGVEGEPASLQTADKKDHLKKAEKDTNSKASKAMLH

Target ORF information:

RefSeq Version	XM_011241735.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus remodeling and spacing factor 1 (Rsf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011241735.2

ORF Insert Sequence:

ATGGCGGCGG CGGCGGCGGC GGCGGCGGCG GCAGCGGCAG CGGCGATGGC TCCTCCGGGC 
TGCCCGGGTT CGTGCCCCAA CTTCGCCGTA GTCTGCTCCT TCTTGGAACG CTACGGGCCG 
CTGCTGGACC TGCCCGAGTT GCCGTTCCCA GAGCTGGAGC GGGTGCTGCA GGCGCCGCCG 
CCAGACATCG GCAACGGAGA AGTACCAAAA GAATTGGTGG AGCTGCATTT GAAGTTAATG 
AGGAAGATTG GCAAATCCGT TACAGCAGAC AGATGGGAAA AATACTTGAT CAAGATCTGT 
CAAGAGTTTA ACAGTACCTG GGCATGGGAG ATGGAGAAGA AAGGCTATCT TGAAATGAGT 
GTTGAATGCA AACTAGCACT CTTAAAGTAT CTCTGTGAGT GTCAGTTTGA TGACAATCTT 
AAATTCAAGA ATATAATTAA TGAGGAGGAT GCTGATACTA TGCGTCTCCA GCCAATTGGG 
CGAGACAAAG ATGGCCTCAT GTATTGGTAC CAGTTGGATC AAGACCATAA TGTGAGAATG 
TACATAGAAG AACAAGATGA TCAAGATGGC TCTTCTTGGA AATGCATTGT CAGAAATCGA 
AACGAGTTGG CTGAGACTCT TGCACTCCTG AAAGCACAAA TTGATCCTGT CCTATTGAAA 
AACTCGAGCC AACAGGAAGA TTCTTCTCGG GAAAGCCCCA CCCTAGAGGA TGAGGAGACT 
AAGAAAGATG AAGAAACACC TAAACAAGAA GCATCAAAAG AAAATGAAAA GACAGGAGCT 
GATGAGCGGT CAGTGGATTC TGGGAAGTGT TCTGCAGCCA GCGCTCTAGA AGAACCAGCT 
GTGAAAACTG AGAAAGCAGA TGAAAAGGAG CTTGCGAAAC TGCCAGTCAT TGTAAAGCTA 
GAAAAGCCTT TGCCAGAAAC TGAGGAAAAA AAGATTATCA GAGAGGAAAG TGATTCTTTC 
AAGGAAAATG TCAAACCCAT TAAAGTAGAA GCAAAGGAAT GCAGAGCGGA CCCAAAAGAT 
CTCAAAGGCA GCTTGGAGCG GCTGGGCTCG CAGGAGCCTG AGCGAGCCGA CTTTGGTAGC 
AACATCAAAT CTCAAGACAT CATAGAGAAA TCTACTGAGG AAACAGAGAA GCTTAAAAAT 
GATCAGCAAG CCAAGATACC ACTGAAGAAA CGTGAAATTA AGCTGAGTGA TGATTTTGAC 
AGTCCAGTCA AAGGACCTTT GTGTAAGTCA GCTACTCCAA CTAAAGAAGT TTTGAAAGAT 
GACATAAAAC AAGAGGAAGA GACTTGTAAA CGGGTCTCTA CCATCAGTGC TTTGAGTCAT 
GAAGGGAAAC AACTTGTGAA TGGAGAGATT AATGATGACA AGGTAATTCC AAATTTTAAG 
ACAGAACAAA TGGAAATACA GCTTTGTGAT ACAAAAGAAG ATAGTGCTAG CATCCCTGCT 
AAGGATGGAA ATGCCTGCAT GGAGGGAAAT GGTACGGAGT GCTTAAATTC TGTCATAACC 
AGCACAAAGA CAAGTGAGCT TGAGAAAGAG GCCCCTCTAG GGAAGGGTAT AGACAGCTCT 
GTACCTGACA TAGAGAGCCT CAGTCAGAAA GCAAGGTTAG AGGAGCCTGG GCTTCTGAAC 
ATGGACATGC CTCTCGAACC TTCTGAGAAA GCCACGGATC TCTCTTTAAA ACCCACTTTG 
TCTGCCACAG AACCCTGCAG TACGAAAGTT GAAGAGAAGG CCCCCAGAAG TAAGAGTGAG 
AACCACACCC CAGGCATAGA ATGCCTGGAA AGGGTGGAGA AAGCCAAGAA GACCTCCATT 
GATAAGGACA TCCAAAAATT GAGTCCAATA CCAGAAGAAG TTGTAAGGGG TGCTCTAGAG 
TCAGAAAAGT CAGGTCCTTG TGAAGTGGCT GAAACTCCTC TCCCACTCTC GATGGCTGGG 
GCCAAGGAGA AACAGACCTC AGAAAAAAAG GATGTAGACT GTAGTAGAGG TTCGAGTGAA 
AGTCAGTCCC TTGAAAATGC AAGCCCAGAA ATTCTAAAAG AGGATTCTGA GTCCTCGAGG 
GAGGAAGTGG CCAAACTGGA CAATGCCCAG ACCTCTGGCA TGGAGGATAC TTCTGAAACA 
AAGGGCTCTG TGCAGAAAAA CAAATTTAAA TATAAGTTAG TTTCTGAAAG AAACAGTACT 
GCTTCAGAAA ACACTGAGAT CACCTCTGAA AGGAAGAAGG AAGGCATCAA GTTGACAATT 
AGGATATCAA GTCGGAAGAA GAAGCCAGAT TGCCCCCCAC AAACTGTAGA CTCAGAAAGC 
AAGGAAGAGA AGGCGGGCAA GGAAGAAGAG AAAGCAAGTG TGGGTCGAAC TTTAAGAAGG 
TCTCCAAGAA TATCTAGACC CACAGCAAAA GTCGCTGAGA TCAGAGTCCA GAAAGCCGAT 
AAGAGAAGAG CTGACGGAGA AGATGGAGTG GAAGGGGAGC CGGCATCTTT GCAAACAGCA 
GACAAGAAGG ATCACTTGAA GAAAGCAGAG AAGGATACAA ATTCTAAAGC AAGCAAGGCA 
ATGCTGCATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Role of remodeling and spacing factor 1 in histone H2A ubiquitination-mediated gene silencing.
Proceedings of the National Academy of Sciences of the United States of America114(38)E7949-E7958(2017 Sep)
Zhang Z,Jones AE,Wu W,Kim J,Kang Y,Bi X,Gu Y,Popov IK,Renfrow MB,Vassylyeva MN,Vassylyev DG,Giles KE,Chen D,Kumar A,Fan Y,Tong Y,Liu CF,An W,Chang C,Luo J,Chow LT,Wang H

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Specification of jaw identity by the Hand2 transcription factor.
Scientific reports628405(2016 Jun)
Funato N,Kokubo H,Nakamura M,Yanagisawa H,Saga Y

Specific inhibition of gene expression and transactivation functions of hepatitis B virus X protein and c-myc by small interfering RNAs.
FEBS letters560(1-3)210-4(2004 Feb)
Hung L,Kumar V

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Rsf1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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