Arhgap35 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Arhgap35
Entrez Gene ID	232906
Full Name	Rho GTPase activating protein 35
Synonyms	6430596G11Rik,AI841135,Grlf1,mKIAA1722,p190A,p190RhoGAP
General protein information	Preferred Names Rho GTPase activating protein 35 Names rho GTPase-activating protein 35 P190 RhoGAP glucocorticoid receptor DNA-binding factor 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 A2

mRNA and Protein(s)

mRNA	Protein	Name
XM_006539809.3	XP_006539872.1	rho GTPase-activating protein 35 isoform X1
XM_011250512.2	XP_011248814.1	rho GTPase-activating protein 35 isoform X1
XM_011250512.3	XP_011248814.1	rho GTPase-activating protein 35 isoform X1
XM_006539809.4	XP_006539872.1	rho GTPase-activating protein 35 isoform X1
NM_172739.4	NP_766327.3	rho GTPase-activating protein 35

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ARHGAP35	XP_003316510.1
	GRLF1	XP_001112719.1
Rattus norvegicus (Norway rat)	Arhgap35	NP_001258061.1
Gallus gallus (chicken)	ARHGAP35	XP_003643808.2
Homo sapiens (human)	ARHGAP35	NP_004482.4
Bos taurus (cattle)	ARHGAP35	NP_001179327.1
Danio rerio (zebrafish)	arhgap35	XP_003198691.2
Danio rerio (zebrafish)	LOC100004299	XP_001343636.1
Xenopus tropicalis (tropical clawed frog)	arhgap35	XP_004917009.1
Drosophila melanogaster (fruit fly)	RhoGAPp190	NP_001259658.1
Mus musculus (house mouse)	Grlf1	NP_766327.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000870	XP_316844.5
Canis lupus familiaris (dog)	ARHGAP35	NP_001003022.1

Gene Ontology
Bibliography

Related articles in PubMed

A Point Mutation in p190A RhoGAP Affects Ciliogenesis and Leads to Glomerulocystic Kidney Defects.
Stewart K, Gaitan Y, Shafer ME, Aoudjit L, Hu D, Sharma R, Tremblay M, Ishii H, Marcotte M, Stanga D, Tang YC, Boualia SK, Nguyen AH, Takano T, Lamarche-Vane N, Vidal S, Bouchard M
PLoS genetics12(2)e1005785(2016 Feb)

An AUTS2-Polycomb complex activates gene expression in the CNS.
Gao Z, Lee P, Stafford JM, von Schimmelmann M, Schaefer A, Reinberg D
Nature516(7531)349-54(2014 Dec)

p190RhoGAP has cellular RacGAP activity regulated by a polybasic region.
L?vay M, Bartos B, Ligeti E
Cellular signalling25(6)1388-94(2013 Jun)

Integrin ?3 is required for late postnatal stability of dendrite arbors, dendritic spines and synapses, and mouse behavior.
Kerrisk ME, Greer CA, Koleske AJ
The Journal of neuroscience : the official journal of the Society for Neuroscience33(16)6742-52(2013 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

p190A acts as a modulator of Rho GTPases in a localized area around the cilia to permit the dynamic actin rearrangement required for cilia elongation.
Title: A Point Mutation in p190A RhoGAP Affects Ciliogenesis and Leads to Glomerulocystic Kidney Defects.

The cellular RacGAP activity of p190RhoGAP requires an intact polybasic region adjacent to the GAP domain whereas the RhoGAP activity is inhibited by the same domain.
Title: p190RhoGAP has cellular RacGAP activity regulated by a polybasic region.

study demonstrates that the epidermal growth factor receptor (EGFR) cooperates with beta3 integrin to regulate p190RhoGAP activity in mammary gland epithelial cells
Title: ?3 integrin-EGF receptor cross-talk activates p190RhoGAP in mouse mammary gland epithelial cells.

p190RhoGAP links integrins and caveolin-1/caveolae to RhoA in a mechanotransduction cascade that participates in endothelial adaptation to flow.
Title: p190 RhoGTPase-activating protein links the ?1 integrin/caveolin-1 mechanosignaling complex to RhoA and actin remodeling.

In a novel knockout mouse strain p190RhoGAP does not play a major, indispensable role in integrin signaling of neutrophils.
Title: Neutrophil functions and autoimmune arthritis in the absence of p190RhoGAP: generation and analysis of a novel null mutation in mice.

The following Arhgap35 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Arhgap35 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu20691	XM_011250512.3 Latest version!	Mus musculus Rho GTPase activating protein 35 (Arhgap35), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu20691	XM_006539809.4 Latest version!	Mus musculus Rho GTPase activating protein 35 (Arhgap35), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu20691	NM_172739.4 Latest version!	Mus musculus Rho GTPase activating protein 35 (Arhgap35), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
View Old Accession Versions >>
OMu20691	XM_011250512.2	Mus musculus Rho GTPase activating protein 35 (Arhgap35), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu20691	XM_006539809.3	Mus musculus Rho GTPase activating protein 35 (Arhgap35), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Arhgap35 ( XM_011250512.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011248814.1 Mus musculus Rho GTPase activating protein 35 (Arhgap35), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu20691
Clone ID Related Accession (Same CDS sequence)	XM_006539809.3 , XM_011250512.2 , XM_011250512.3 , XM_006539809.4 , NM_172739.4
Accession Version	XM_011250512.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rho GTPase-activating protein 35 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011250512.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGG CAAGAAAGCA AGATGTCCGC ATCCCCACCT ACAACATCAG TGTGGTGGGA 
TTGTCAGGCA CTGAGAAGGA GAAAGGCCAG TGCGGCATTG GGAAGTCTTG TCTGTGTAAC 
CGCTTTGTGC GCCCAAGTGC TGATGAGTTT CACCTGGACC ACACTTCTGT CCTTAGCACC 
AGTGACTTTG GTGGGCGGGT GGTCAATAAT GACCACTTTC TGTACTGGGG AGAAGTTAGC 
CGCTCCCTGG AGGATTGTGT GGAATGTAAG ATGCACATTG TGGAGCAGAC TGAGTTTATT 
GACGATCAGA CTTTTCAACC TCATCGAAGC ACGGCACTGC AGCCCTACAT CAAGAGAGCC 
GCAGCCACCA AGCTTGCTTC AGCTGAAAAA CTCATGTATT TTTGCACTGA CCAGCTGGGA 
CTGGAGCAAG ACTTTGAGCA GAAACAGATG CCAGATGGGA AGCTGCTGGT TGACGGTTTC 
CTTCTGGGCA TTGATGTTAG CAGGGGCATG AACAGGAACT TCGATGATCA GCTCAAATTT 
GTCTCCAATC TCTACAATCA GCTTGCAAAA ACAAAAAAGC CCATAGTGGT AGTCCTGACT 
AAGTGTGATG AGGGCGTTGA GCGGTACATT AGAGATGCAC ATACTTTTGC CTTAAGCAAA 
AAGAACCTCC AGGTTGTAGA GACCTCTGCA AGGTCCAATG TGAATGTGGA CTTGGCTTTC 
AGCACCTTAG TGCAACTCAT TGATAAGAGT CGAGGGAAGA CAAAAATCAT CCCTTACTTT 
GAAGCTCTCA AGCAGCAGAG TCAGCAGATA GCTACAGCAA AGGACAAGTA TGAGTGGTTA 
GTGAGCCGCA TTGTGAAAAA TCACAATGAG AACTGGCCGA GTGTCAGCCG AAAGATGCAG 
GCCTCCCCTG AGTACCAGGA CTATGTCTAC CTGGAAGGGA CACAGAAGGC CAAGAAGCTC 
TTCCTGCAGC ACATTCACCG CCTCAAGCAT GAGCATATTG AGCGTCGGAG AAAGCTGTAC 
CTGGCAGCTC TGCCATTGGC TTTTGAAGCC CTCATACCTA ATCTAGATGA AGTGGACCAC 
CTGAGCTGTA TTAAAGCAAA AAAGCTGTTA GAGACCAAGC CAGAGTTCTT AAAGTGGTTT 
GTTGTACTTG AAGAAACACC ATGGGATGCC ACCAGCCACA TTGACAACAT GGAAAATGAG 
CGGATTCCCT TTGACTTGAT GGATACTGTC CCTGCTGAGC AGTTGTATGA GACCCACTTG 
GAGAAGCTGA GGAACGAGAG GAAGAGAGCT GAGATGCGAA GGGCTTTCAA AGAAAACCTG 
GAGACCTCTC CTTTCATAAC TCCTGGGAAA CCTTGGGAAG AAGCTCGTAG TTTTATTATG 
AATGAAGACT TCTACCAGTG GCTGGAAGAA TCTGTGTACA TGGACATCTA TGGCAAGCAC 
CAAAAGCAGA TTATAGACCG AGCAAAGGAA GAGTTCCAAG AGTTGCTTTT GGAGTACTCA 
GAATTGTTTT ATGAGCTGGA GCTGGACGCT AAGCCCAGCA AGGAGAAGAT GGGTGTCATC 
CAGGATGTTC TGGGCGAAGA GCAGAGATTT AAAGCATTGC AGAAGCTCCA AGCAGAGCGT 
GATGCCCTCA TTCTGAAGCA CATCCATTTT GTGTACCACC CGACGAAGGA GACATGCCCA 
AGCTGTCCAG CTTGTGTAGA TGCTAAGATT GAACACTTGA TCAGTTCTCG CTTTATCAGA 
CCATCTGATC GGAATCAGAA GAACTCTCTG TCTGACCTCA ATATTGATAG GATCAATTTG 
GTTATCTTAG GCAAAGATGG CCTTGCCCGA GAGTTGGCCA ATGAAATTCG AGCTCTTTGT 
ACAAATGATG ACAAGTATGT AATAGATGGT AAAATGTATG AGCTTTCTCT GAGGCCAATA 
GAGGGGAATG TCCGGCTTCC TGTGAACTCT TTCCAGACAC CAACCTTCCA ACCCCATGGC 
TGTCTCTGCC TTTACAATTC AAAGGAGTCA CTGTCCTATG TGGTGGAGAG TATAGAGAAG 
AGCAGAGAGT CTACACTGGG CAGGCGGGAT AATCATTTAG TCCACCTTCC CTTGACCTTA 
ATTTTAGTTA ACAAGAGAGG GGACACAAGT GGAGAGACTC TGCACAGCTT GATACAGCAA 
GGTCAGCAGA TTGCCAGCAA ACTTCAGTGT GTCTTTCTTG ATCCGGCTTC TGCTGGCATT 
GGTTATGGAC GCAACATTAA TGAGAAGCAG ATCAGTCAAG TTCTGAAAGG ACTCCTGGAC 
TCCAAGCGCA ACTTAAACCT GGTTAGCTCC ACTGCAAGTA TCAAAGATTT GGCTGATGTG 
GACCTTCGAA TTGTCATGTG TCTGATGTGT GGTGATCCCT TTAGTGCAGA TGACGTTCTC 
TCTCCTGTCC TGCAATCCCA AACTTGTAAA TCTTCCCACT GTGGGAGCAG CAACTCTGTT 
TTACTTGAAC TTCCAATTGG ACTACACAAG AAGCGCATTG AGCTGTCTGT TCTTTCATAC 
CATTCCTCAT TTAGCATCCG AAAGAGCCGG TTGGTTCATG GGTACATTGT TTTTTATTCA 
GCCAAACGTA AGGCCTCCTT GGCAATGTTA CGTGCCTTTC TTTGTGAAGT GCAGGATATT 
ATCCCCATCC AGCTTGTCGC ACTCACTGAT GGCGCTATAG ATGTCCTAGA CAATGACTTA 
AGTCGAGAGC AGCTAACAGA GGGAGAGGAA ATTGCACAAG AGATTGACGG GAGATTCACA 
AGCATCCCTT GTAGCCAGCC CCAGCATAAA CTTGAGCTCT TTCATCCCTT TTTTAAAGAT 
GTGGTGGAGA AAAAGAACAT AATTGAGGCC ACACATATGT ATGATAATGT AGCTGAGGCC 
TGCAGCACCA CTGAGGAAGT GTTCAACTCC CCCAGAGCTG GCTCACCCCT CTGCAACTCA 
AACTTACAGG ACTCAGAAGA AGATGTGGAG CCTCCATCAT ACCACCTGTT TCGGGAAGAT 
GCGACATTGC CCTCCCTGTC CAAAGATCAT TCCAAGTTCT CTATGGAACT GGAGGGAAAT 
GACGGGCTAT CTTTCATAAT GAGCAATTTT GAGAGTAAAC TGAACAACAA AGTACCTCCC 
CCAGTCAAAC CAAAGCCTCC TGTGCATTTT GATATCACAA AAGATCTTTC TTACTTAGAC 
CAAGGCCATC GGGAGGGACA GAGGAAATCT ATGTCTTCTA GCCCCTGGAT GCCTCAGGAT 
GGGTTTGATC CTTCTGACTA CGCAGAGCCC ATGGATGCTG TGGTCAAGCC AAGGAATGAG 
GAAGAAAACA TATACTCGGT GCCCCACGAC AGCACCCAGG GCAAGATCAT TACCATTCGG 
AACATCAACA AAGCCCAGTC CAATGGCAGT GGTAATGGTT CTGACAGTGA GATGGACACA 
AGTTCCCTAG AGCGAGGCCG CAAGGTATCT GCAGTGAGTA AGCCTGTGCT GTACAGGACA 
AGATGCACTC GCCTGGGGCG GTTTGCTAGT TACCGTACCA GCTTCAGTGT CGGGAGTGAT 
GATGAGCTGG GACCCATCCG AAAGAAAGAG GAGGACCAGG CATCCCAAGG TTATAAAGGG 
GACAATGCTG TCATTCCATA TGAAACAGAT GAGGACCCCA GGAGGAGGAA TATCCTTCGA 
AGTCTAAGAA GGAACACCAA GAAACCAAAG CCCAAACCCC GGCCATCCAT CACAAAGGCA 
ACCTGGGAGA GTAACTATTT TGGGGTGCCC TTAACAACAG TGGTGACTCC AGAGAAACCG 
ATACCCATTT TCATTGAAAG ATGCATTGAG TACATCGAAG CCACAGGACT AAGCACTGAA 
GGCATCTACC GGGTCAGTGG AAACAAGTCA GAAATGGAAA GTTTGCAAAG ACAGTTTGAT 
CAAGACCACA ATCTGGACCT GGCAGAGAAA GACTTCACTG TGAACACTGT GGCAGGGGCC 
ATGAAGAGTT TTTTCTCAGA GCTACCAGAC CCCCTGGTAC CATACAGCAT GCAGATTGAC 
TTGGTGGAAG CTCACAAAAT CAACGACAGG GAACAGAAGC TGCATGCTCT GAAGGAAGTG 
CTGAAGAAGT TCCCGAAGGA AAACCATGAA GTCTTCAAAT ATGTCATCTC CCACCTGAAC 
AAAGTCAGCC ACAACAACAA GGTGAATCTT ATGACCAGTG AGAACCTGTC CATCTGCTTC 
TGGCCCACGC TGATGCGGCC TGACTTCAGC AGCATGGACG CGCTCACAGC CACGCGCTCC 
TACCAGACCA TCATCGAGCT CTTCATCCAG CAGTGCCCCT TCTTCTTCTA CAACCGGCCC 
ATCAGCGAGC CTCCAGGGGC TGCGCCTGGC TCCCCTTCAG CCATGGCTCC CACTGTTCCC 
TTCCTCACCT CTACACCTGC TACCAGTCAG CCATCACCTC CCCAGTCACC TCCTCCAACC 
CCTCAGTCCC CAATGCAGCC ATTGCTGTCC TCTCAGCTCC AAGCCGAACA CACGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011248814.1
CDS	446..4945
Translation	MMMARKQDVRIPTYNISVVGLSGTEKEKGQCGIGKSCLCNRFVRPSADEFHLDHTSVLSTSDFGGRVVNNDHFLYWGEVSRSLEDCVECKMHIVEQTEFIDDQTFQPHRSTALQPYIKRAAATKLASAEKLMYFCTDQLGLEQDFEQKQMPDGKLLVDGFLLGIDVSRGMNRNFDDQLKFVSNLYNQLAKTKKPIVVVLTKCDEGVERYIRDAHTFALSKKNLQVVETSARSNVNVDLAFSTLVQLIDKSRGKTKIIPYFEALKQQSQQIATAKDKYEWLVSRIVKNHNENWPSVSRKMQASPEYQDYVYLEGTQKAKKLFLQHIHRLKHEHIERRRKLYLAALPLAFEALIPNLDEVDHLSCIKAKKLLETKPEFLKWFVVLEETPWDATSHIDNMENERIPFDLMDTVPAEQLYETHLEKLRNERKRAEMRRAFKENLETSPFITPGKPWEEARSFIMNEDFYQWLEESVYMDIYGKHQKQIIDRAKEEFQELLLEYSELFYELELDAKPSKEKMGVIQDVLGEEQRFKALQKLQAERDALILKHIHFVYHPTKETCPSCPACVDAKIEHLISSRFIRPSDRNQKNSLSDLNIDRINLVILGKDGLARELANEIRALCTNDDKYVIDGKMYELSLRPIEGNVRLPVNSFQTPTFQPHGCLCLYNSKESLSYVVESIEKSRESTLGRRDNHLVHLPLTLILVNKRGDTSGETLHSLIQQGQQIASKLQCVFLDPASAGIGYGRNINEKQISQVLKGLLDSKRNLNLVSSTASIKDLADVDLRIVMCLMCGDPFSADDVLSPVLQSQTCKSSHCGSSNSVLLELPIGLHKKRIELSVLSYHSSFSIRKSRLVHGYIVFYSAKRKASLAMLRAFLCEVQDIIPIQLVALTDGAIDVLDNDLSREQLTEGEEIAQEIDGRFTSIPCSQPQHKLELFHPFFKDVVEKKNIIEATHMYDNVAEACSTTEEVFNSPRAGSPLCNSNLQDSEEDVEPPSYHLFREDATLPSLSKDHSKFSMELEGNDGLSFIMSNFESKLNNKVPPPVKPKPPVHFDITKDLSYLDQGHREGQRKSMSSSPWMPQDGFDPSDYAEPMDAVVKPRNEEENIYSVPHDSTQGKIITIRNINKAQSNGSGNGSDSEMDTSSLERGRKVSAVSKPVLYRTRCTRLGRFASYRTSFSVGSDDELGPIRKKEEDQASQGYKGDNAVIPYETDEDPRRRNILRSLRRNTKKPKPKPRPSITKATWESNYFGVPLTTVVTPEKPIPIFIERCIEYIEATGLSTEGIYRVSGNKSEMESLQRQFDQDHNLDLAEKDFTVNTVAGAMKSFFSELPDPLVPYSMQIDLVEAHKINDREQKLHALKEVLKKFPKENHEVFKYVISHLNKVSHNNKVNLMTSENLSICFWPTLMRPDFSSMDALTATRSYQTIIELFIQQCPFFFYNRPISEPPGAAPGSPSAMAPTVPFLTSTPATSQPSPPQSPPPTPQSPMQPLLSSQLQAEHTL

Target ORF information:

RefSeq Version	XM_011250512.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho GTPase activating protein 35 (Arhgap35), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011250512.3

ORF Insert Sequence:

ATGATGATGG CAAGAAAGCA AGATGTCCGC ATCCCCACCT ACAACATCAG TGTGGTGGGA 
TTGTCAGGCA CTGAGAAGGA GAAAGGCCAG TGCGGCATTG GGAAGTCTTG TCTGTGTAAC 
CGCTTTGTGC GCCCAAGTGC TGATGAGTTT CACCTGGACC ACACTTCTGT CCTTAGCACC 
AGTGACTTTG GTGGGCGGGT GGTCAATAAT GACCACTTTC TGTACTGGGG AGAAGTTAGC 
CGCTCCCTGG AGGATTGTGT GGAATGTAAG ATGCACATTG TGGAGCAGAC TGAGTTTATT 
GACGATCAGA CTTTTCAACC TCATCGAAGC ACGGCACTGC AGCCCTACAT CAAGAGAGCC 
GCAGCCACCA AGCTTGCTTC AGCTGAAAAA CTCATGTATT TTTGCACTGA CCAGCTGGGA 
CTGGAGCAAG ACTTTGAGCA GAAACAGATG CCAGATGGGA AGCTGCTGGT TGACGGTTTC 
CTTCTGGGCA TTGATGTTAG CAGGGGCATG AACAGGAACT TCGATGATCA GCTCAAATTT 
GTCTCCAATC TCTACAATCA GCTTGCAAAA ACAAAAAAGC CCATAGTGGT AGTCCTGACT 
AAGTGTGATG AGGGCGTTGA GCGGTACATT AGAGATGCAC ATACTTTTGC CTTAAGCAAA 
AAGAACCTCC AGGTTGTAGA GACCTCTGCA AGGTCCAATG TGAATGTGGA CTTGGCTTTC 
AGCACCTTAG TGCAACTCAT TGATAAGAGT CGAGGGAAGA CAAAAATCAT CCCTTACTTT 
GAAGCTCTCA AGCAGCAGAG TCAGCAGATA GCTACAGCAA AGGACAAGTA TGAGTGGTTA 
GTGAGCCGCA TTGTGAAAAA TCACAATGAG AACTGGCCGA GTGTCAGCCG AAAGATGCAG 
GCCTCCCCTG AGTACCAGGA CTATGTCTAC CTGGAAGGGA CACAGAAGGC CAAGAAGCTC 
TTCCTGCAGC ACATTCACCG CCTCAAGCAT GAGCATATTG AGCGTCGGAG AAAGCTGTAC 
CTGGCAGCTC TGCCATTGGC TTTTGAAGCC CTCATACCTA ATCTAGATGA AGTGGACCAC 
CTGAGCTGTA TTAAAGCAAA AAAGCTGTTA GAGACCAAGC CAGAGTTCTT AAAGTGGTTT 
GTTGTACTTG AAGAAACACC ATGGGATGCC ACCAGCCACA TTGACAACAT GGAAAATGAG 
CGGATTCCCT TTGACTTGAT GGATACTGTC CCTGCTGAGC AGTTGTATGA GACCCACTTG 
GAGAAGCTGA GGAACGAGAG GAAGAGAGCT GAGATGCGAA GGGCTTTCAA AGAAAACCTG 
GAGACCTCTC CTTTCATAAC TCCTGGGAAA CCTTGGGAAG AAGCTCGTAG TTTTATTATG 
AATGAAGACT TCTACCAGTG GCTGGAAGAA TCTGTGTACA TGGACATCTA TGGCAAGCAC 
CAAAAGCAGA TTATAGACCG AGCAAAGGAA GAGTTCCAAG AGTTGCTTTT GGAGTACTCA 
GAATTGTTTT ATGAGCTGGA GCTGGACGCT AAGCCCAGCA AGGAGAAGAT GGGTGTCATC 
CAGGATGTTC TGGGCGAAGA GCAGAGATTT AAAGCATTGC AGAAGCTCCA AGCAGAGCGT 
GATGCCCTCA TTCTGAAGCA CATCCATTTT GTGTACCACC CGACGAAGGA GACATGCCCA 
AGCTGTCCAG CTTGTGTAGA TGCTAAGATT GAACACTTGA TCAGTTCTCG CTTTATCAGA 
CCATCTGATC GGAATCAGAA GAACTCTCTG TCTGACCTCA ATATTGATAG GATCAATTTG 
GTTATCTTAG GCAAAGATGG CCTTGCCCGA GAGTTGGCCA ATGAAATTCG AGCTCTTTGT 
ACAAATGATG ACAAGTATGT AATAGATGGT AAAATGTATG AGCTTTCTCT GAGGCCAATA 
GAGGGGAATG TCCGGCTTCC TGTGAACTCT TTCCAGACAC CAACCTTCCA ACCCCATGGC 
TGTCTCTGCC TTTACAATTC AAAGGAGTCA CTGTCCTATG TGGTGGAGAG TATAGAGAAG 
AGCAGAGAGT CTACACTGGG CAGGCGGGAT AATCATTTAG TCCACCTTCC CTTGACCTTA 
ATTTTAGTTA ACAAGAGAGG GGACACAAGT GGAGAGACTC TGCACAGCTT GATACAGCAA 
GGTCAGCAGA TTGCCAGCAA ACTTCAGTGT GTCTTTCTTG ATCCGGCTTC TGCTGGCATT 
GGTTATGGAC GCAACATTAA TGAGAAGCAG ATCAGTCAAG TTCTGAAAGG ACTCCTGGAC 
TCCAAGCGCA ACTTAAACCT GGTTAGCTCC ACTGCAAGTA TCAAAGATTT GGCTGATGTG 
GACCTTCGAA TTGTCATGTG TCTGATGTGT GGTGATCCCT TTAGTGCAGA TGACGTTCTC 
TCTCCTGTCC TGCAATCCCA AACTTGTAAA TCTTCCCACT GTGGGAGCAG CAACTCTGTT 
TTACTTGAAC TTCCAATTGG ACTACACAAG AAGCGCATTG AGCTGTCTGT TCTTTCATAC 
CATTCCTCAT TTAGCATCCG AAAGAGCCGG TTGGTTCATG GGTACATTGT TTTTTATTCA 
GCCAAACGTA AGGCCTCCTT GGCAATGTTA CGTGCCTTTC TTTGTGAAGT GCAGGATATT 
ATCCCCATCC AGCTTGTCGC ACTCACTGAT GGCGCTATAG ATGTCCTAGA CAATGACTTA 
AGTCGAGAGC AGCTAACAGA GGGAGAGGAA ATTGCACAAG AGATTGACGG GAGATTCACA 
AGCATCCCTT GTAGCCAGCC CCAGCATAAA CTTGAGCTCT TTCATCCCTT TTTTAAAGAT 
GTGGTGGAGA AAAAGAACAT AATTGAGGCC ACACATATGT ATGATAATGT AGCTGAGGCC 
TGCAGCACCA CTGAGGAAGT GTTCAACTCC CCCAGAGCTG GCTCACCCCT CTGCAACTCA 
AACTTACAGG ACTCAGAAGA AGATGTGGAG CCTCCATCAT ACCACCTGTT TCGGGAAGAT 
GCGACATTGC CCTCCCTGTC CAAAGATCAT TCCAAGTTCT CTATGGAACT GGAGGGAAAT 
GACGGGCTAT CTTTCATAAT GAGCAATTTT GAGAGTAAAC TGAACAACAA AGTACCTCCC 
CCAGTCAAAC CAAAGCCTCC TGTGCATTTT GATATCACAA AAGATCTTTC TTACTTAGAC 
CAAGGCCATC GGGAGGGACA GAGGAAATCT ATGTCTTCTA GCCCCTGGAT GCCTCAGGAT 
GGGTTTGATC CTTCTGACTA CGCAGAGCCC ATGGATGCTG TGGTCAAGCC AAGGAATGAG 
GAAGAAAACA TATACTCGGT GCCCCACGAC AGCACCCAGG GCAAGATCAT TACCATTCGG 
AACATCAACA AAGCCCAGTC CAATGGCAGT GGTAATGGTT CTGACAGTGA GATGGACACA 
AGTTCCCTAG AGCGAGGCCG CAAGGTATCT GCAGTGAGTA AGCCTGTGCT GTACAGGACA 
AGATGCACTC GCCTGGGGCG GTTTGCTAGT TACCGTACCA GCTTCAGTGT CGGGAGTGAT 
GATGAGCTGG GACCCATCCG AAAGAAAGAG GAGGACCAGG CATCCCAAGG TTATAAAGGG 
GACAATGCTG TCATTCCATA TGAAACAGAT GAGGACCCCA GGAGGAGGAA TATCCTTCGA 
AGTCTAAGAA GGAACACCAA GAAACCAAAG CCCAAACCCC GGCCATCCAT CACAAAGGCA 
ACCTGGGAGA GTAACTATTT TGGGGTGCCC TTAACAACAG TGGTGACTCC AGAGAAACCG 
ATACCCATTT TCATTGAAAG ATGCATTGAG TACATCGAAG CCACAGGACT AAGCACTGAA 
GGCATCTACC GGGTCAGTGG AAACAAGTCA GAAATGGAAA GTTTGCAAAG ACAGTTTGAT 
CAAGACCACA ATCTGGACCT GGCAGAGAAA GACTTCACTG TGAACACTGT GGCAGGGGCC 
ATGAAGAGTT TTTTCTCAGA GCTACCAGAC CCCCTGGTAC CATACAGCAT GCAGATTGAC 
TTGGTGGAAG CTCACAAAAT CAACGACAGG GAACAGAAGC TGCATGCTCT GAAGGAAGTG 
CTGAAGAAGT TCCCGAAGGA AAACCATGAA GTCTTCAAAT ATGTCATCTC CCACCTGAAC 
AAAGTCAGCC ACAACAACAA GGTGAATCTT ATGACCAGTG AGAACCTGTC CATCTGCTTC 
TGGCCCACGC TGATGCGGCC TGACTTCAGC AGCATGGACG CGCTCACAGC CACGCGCTCC 
TACCAGACCA TCATCGAGCT CTTCATCCAG CAGTGCCCCT TCTTCTTCTA CAACCGGCCC 
ATCAGCGAGC CTCCAGGGGC TGCGCCTGGC TCCCCTTCAG CCATGGCTCC CACTGTTCCC 
TTCCTCACCT CTACACCTGC TACCAGTCAG CCATCACCTC CCCAGTCACC TCCTCCAACC 
CCTCAGTCCC CAATGCAGCC ATTGCTGTCC TCTCAGCTCC AAGCCGAACA CACGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgap35 ( XM_006539809.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006539872.1 Mus musculus Rho GTPase activating protein 35 (Arhgap35), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu20691
Clone ID Related Accession (Same CDS sequence)	XM_006539809.3 , XM_011250512.2 , XM_011250512.3 , XM_006539809.4 , NM_172739.4
Accession Version	XM_006539809.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rho GTPase-activating protein 35 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006539809.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGG CAAGAAAGCA AGATGTCCGC ATCCCCACCT ACAACATCAG TGTGGTGGGA 
TTGTCAGGCA CTGAGAAGGA GAAAGGCCAG TGCGGCATTG GGAAGTCTTG TCTGTGTAAC 
CGCTTTGTGC GCCCAAGTGC TGATGAGTTT CACCTGGACC ACACTTCTGT CCTTAGCACC 
AGTGACTTTG GTGGGCGGGT GGTCAATAAT GACCACTTTC TGTACTGGGG AGAAGTTAGC 
CGCTCCCTGG AGGATTGTGT GGAATGTAAG ATGCACATTG TGGAGCAGAC TGAGTTTATT 
GACGATCAGA CTTTTCAACC TCATCGAAGC ACGGCACTGC AGCCCTACAT CAAGAGAGCC 
GCAGCCACCA AGCTTGCTTC AGCTGAAAAA CTCATGTATT TTTGCACTGA CCAGCTGGGA 
CTGGAGCAAG ACTTTGAGCA GAAACAGATG CCAGATGGGA AGCTGCTGGT TGACGGTTTC 
CTTCTGGGCA TTGATGTTAG CAGGGGCATG AACAGGAACT TCGATGATCA GCTCAAATTT 
GTCTCCAATC TCTACAATCA GCTTGCAAAA ACAAAAAAGC CCATAGTGGT AGTCCTGACT 
AAGTGTGATG AGGGCGTTGA GCGGTACATT AGAGATGCAC ATACTTTTGC CTTAAGCAAA 
AAGAACCTCC AGGTTGTAGA GACCTCTGCA AGGTCCAATG TGAATGTGGA CTTGGCTTTC 
AGCACCTTAG TGCAACTCAT TGATAAGAGT CGAGGGAAGA CAAAAATCAT CCCTTACTTT 
GAAGCTCTCA AGCAGCAGAG TCAGCAGATA GCTACAGCAA AGGACAAGTA TGAGTGGTTA 
GTGAGCCGCA TTGTGAAAAA TCACAATGAG AACTGGCCGA GTGTCAGCCG AAAGATGCAG 
GCCTCCCCTG AGTACCAGGA CTATGTCTAC CTGGAAGGGA CACAGAAGGC CAAGAAGCTC 
TTCCTGCAGC ACATTCACCG CCTCAAGCAT GAGCATATTG AGCGTCGGAG AAAGCTGTAC 
CTGGCAGCTC TGCCATTGGC TTTTGAAGCC CTCATACCTA ATCTAGATGA AGTGGACCAC 
CTGAGCTGTA TTAAAGCAAA AAAGCTGTTA GAGACCAAGC CAGAGTTCTT AAAGTGGTTT 
GTTGTACTTG AAGAAACACC ATGGGATGCC ACCAGCCACA TTGACAACAT GGAAAATGAG 
CGGATTCCCT TTGACTTGAT GGATACTGTC CCTGCTGAGC AGTTGTATGA GACCCACTTG 
GAGAAGCTGA GGAACGAGAG GAAGAGAGCT GAGATGCGAA GGGCTTTCAA AGAAAACCTG 
GAGACCTCTC CTTTCATAAC TCCTGGGAAA CCTTGGGAAG AAGCTCGTAG TTTTATTATG 
AATGAAGACT TCTACCAGTG GCTGGAAGAA TCTGTGTACA TGGACATCTA TGGCAAGCAC 
CAAAAGCAGA TTATAGACCG AGCAAAGGAA GAGTTCCAAG AGTTGCTTTT GGAGTACTCA 
GAATTGTTTT ATGAGCTGGA GCTGGACGCT AAGCCCAGCA AGGAGAAGAT GGGTGTCATC 
CAGGATGTTC TGGGCGAAGA GCAGAGATTT AAAGCATTGC AGAAGCTCCA AGCAGAGCGT 
GATGCCCTCA TTCTGAAGCA CATCCATTTT GTGTACCACC CGACGAAGGA GACATGCCCA 
AGCTGTCCAG CTTGTGTAGA TGCTAAGATT GAACACTTGA TCAGTTCTCG CTTTATCAGA 
CCATCTGATC GGAATCAGAA GAACTCTCTG TCTGACCTCA ATATTGATAG GATCAATTTG 
GTTATCTTAG GCAAAGATGG CCTTGCCCGA GAGTTGGCCA ATGAAATTCG AGCTCTTTGT 
ACAAATGATG ACAAGTATGT AATAGATGGT AAAATGTATG AGCTTTCTCT GAGGCCAATA 
GAGGGGAATG TCCGGCTTCC TGTGAACTCT TTCCAGACAC CAACCTTCCA ACCCCATGGC 
TGTCTCTGCC TTTACAATTC AAAGGAGTCA CTGTCCTATG TGGTGGAGAG TATAGAGAAG 
AGCAGAGAGT CTACACTGGG CAGGCGGGAT AATCATTTAG TCCACCTTCC CTTGACCTTA 
ATTTTAGTTA ACAAGAGAGG GGACACAAGT GGAGAGACTC TGCACAGCTT GATACAGCAA 
GGTCAGCAGA TTGCCAGCAA ACTTCAGTGT GTCTTTCTTG ATCCGGCTTC TGCTGGCATT 
GGTTATGGAC GCAACATTAA TGAGAAGCAG ATCAGTCAAG TTCTGAAAGG ACTCCTGGAC 
TCCAAGCGCA ACTTAAACCT GGTTAGCTCC ACTGCAAGTA TCAAAGATTT GGCTGATGTG 
GACCTTCGAA TTGTCATGTG TCTGATGTGT GGTGATCCCT TTAGTGCAGA TGACGTTCTC 
TCTCCTGTCC TGCAATCCCA AACTTGTAAA TCTTCCCACT GTGGGAGCAG CAACTCTGTT 
TTACTTGAAC TTCCAATTGG ACTACACAAG AAGCGCATTG AGCTGTCTGT TCTTTCATAC 
CATTCCTCAT TTAGCATCCG AAAGAGCCGG TTGGTTCATG GGTACATTGT TTTTTATTCA 
GCCAAACGTA AGGCCTCCTT GGCAATGTTA CGTGCCTTTC TTTGTGAAGT GCAGGATATT 
ATCCCCATCC AGCTTGTCGC ACTCACTGAT GGCGCTATAG ATGTCCTAGA CAATGACTTA 
AGTCGAGAGC AGCTAACAGA GGGAGAGGAA ATTGCACAAG AGATTGACGG GAGATTCACA 
AGCATCCCTT GTAGCCAGCC CCAGCATAAA CTTGAGCTCT TTCATCCCTT TTTTAAAGAT 
GTGGTGGAGA AAAAGAACAT AATTGAGGCC ACACATATGT ATGATAATGT AGCTGAGGCC 
TGCAGCACCA CTGAGGAAGT GTTCAACTCC CCCAGAGCTG GCTCACCCCT CTGCAACTCA 
AACTTACAGG ACTCAGAAGA AGATGTGGAG CCTCCATCAT ACCACCTGTT TCGGGAAGAT 
GCGACATTGC CCTCCCTGTC CAAAGATCAT TCCAAGTTCT CTATGGAACT GGAGGGAAAT 
GACGGGCTAT CTTTCATAAT GAGCAATTTT GAGAGTAAAC TGAACAACAA AGTACCTCCC 
CCAGTCAAAC CAAAGCCTCC TGTGCATTTT GATATCACAA AAGATCTTTC TTACTTAGAC 
CAAGGCCATC GGGAGGGACA GAGGAAATCT ATGTCTTCTA GCCCCTGGAT GCCTCAGGAT 
GGGTTTGATC CTTCTGACTA CGCAGAGCCC ATGGATGCTG TGGTCAAGCC AAGGAATGAG 
GAAGAAAACA TATACTCGGT GCCCCACGAC AGCACCCAGG GCAAGATCAT TACCATTCGG 
AACATCAACA AAGCCCAGTC CAATGGCAGT GGTAATGGTT CTGACAGTGA GATGGACACA 
AGTTCCCTAG AGCGAGGCCG CAAGGTATCT GCAGTGAGTA AGCCTGTGCT GTACAGGACA 
AGATGCACTC GCCTGGGGCG GTTTGCTAGT TACCGTACCA GCTTCAGTGT CGGGAGTGAT 
GATGAGCTGG GACCCATCCG AAAGAAAGAG GAGGACCAGG CATCCCAAGG TTATAAAGGG 
GACAATGCTG TCATTCCATA TGAAACAGAT GAGGACCCCA GGAGGAGGAA TATCCTTCGA 
AGTCTAAGAA GGAACACCAA GAAACCAAAG CCCAAACCCC GGCCATCCAT CACAAAGGCA 
ACCTGGGAGA GTAACTATTT TGGGGTGCCC TTAACAACAG TGGTGACTCC AGAGAAACCG 
ATACCCATTT TCATTGAAAG ATGCATTGAG TACATCGAAG CCACAGGACT AAGCACTGAA 
GGCATCTACC GGGTCAGTGG AAACAAGTCA GAAATGGAAA GTTTGCAAAG ACAGTTTGAT 
CAAGACCACA ATCTGGACCT GGCAGAGAAA GACTTCACTG TGAACACTGT GGCAGGGGCC 
ATGAAGAGTT TTTTCTCAGA GCTACCAGAC CCCCTGGTAC CATACAGCAT GCAGATTGAC 
TTGGTGGAAG CTCACAAAAT CAACGACAGG GAACAGAAGC TGCATGCTCT GAAGGAAGTG 
CTGAAGAAGT TCCCGAAGGA AAACCATGAA GTCTTCAAAT ATGTCATCTC CCACCTGAAC 
AAAGTCAGCC ACAACAACAA GGTGAATCTT ATGACCAGTG AGAACCTGTC CATCTGCTTC 
TGGCCCACGC TGATGCGGCC TGACTTCAGC AGCATGGACG CGCTCACAGC CACGCGCTCC 
TACCAGACCA TCATCGAGCT CTTCATCCAG CAGTGCCCCT TCTTCTTCTA CAACCGGCCC 
ATCAGCGAGC CTCCAGGGGC TGCGCCTGGC TCCCCTTCAG CCATGGCTCC CACTGTTCCC 
TTCCTCACCT CTACACCTGC TACCAGTCAG CCATCACCTC CCCAGTCACC TCCTCCAACC 
CCTCAGTCCC CAATGCAGCC ATTGCTGTCC TCTCAGCTCC AAGCCGAACA CACGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006539872.1
CDS	385..4884
Translation	MMMARKQDVRIPTYNISVVGLSGTEKEKGQCGIGKSCLCNRFVRPSADEFHLDHTSVLSTSDFGGRVVNNDHFLYWGEVSRSLEDCVECKMHIVEQTEFIDDQTFQPHRSTALQPYIKRAAATKLASAEKLMYFCTDQLGLEQDFEQKQMPDGKLLVDGFLLGIDVSRGMNRNFDDQLKFVSNLYNQLAKTKKPIVVVLTKCDEGVERYIRDAHTFALSKKNLQVVETSARSNVNVDLAFSTLVQLIDKSRGKTKIIPYFEALKQQSQQIATAKDKYEWLVSRIVKNHNENWPSVSRKMQASPEYQDYVYLEGTQKAKKLFLQHIHRLKHEHIERRRKLYLAALPLAFEALIPNLDEVDHLSCIKAKKLLETKPEFLKWFVVLEETPWDATSHIDNMENERIPFDLMDTVPAEQLYETHLEKLRNERKRAEMRRAFKENLETSPFITPGKPWEEARSFIMNEDFYQWLEESVYMDIYGKHQKQIIDRAKEEFQELLLEYSELFYELELDAKPSKEKMGVIQDVLGEEQRFKALQKLQAERDALILKHIHFVYHPTKETCPSCPACVDAKIEHLISSRFIRPSDRNQKNSLSDLNIDRINLVILGKDGLARELANEIRALCTNDDKYVIDGKMYELSLRPIEGNVRLPVNSFQTPTFQPHGCLCLYNSKESLSYVVESIEKSRESTLGRRDNHLVHLPLTLILVNKRGDTSGETLHSLIQQGQQIASKLQCVFLDPASAGIGYGRNINEKQISQVLKGLLDSKRNLNLVSSTASIKDLADVDLRIVMCLMCGDPFSADDVLSPVLQSQTCKSSHCGSSNSVLLELPIGLHKKRIELSVLSYHSSFSIRKSRLVHGYIVFYSAKRKASLAMLRAFLCEVQDIIPIQLVALTDGAIDVLDNDLSREQLTEGEEIAQEIDGRFTSIPCSQPQHKLELFHPFFKDVVEKKNIIEATHMYDNVAEACSTTEEVFNSPRAGSPLCNSNLQDSEEDVEPPSYHLFREDATLPSLSKDHSKFSMELEGNDGLSFIMSNFESKLNNKVPPPVKPKPPVHFDITKDLSYLDQGHREGQRKSMSSSPWMPQDGFDPSDYAEPMDAVVKPRNEEENIYSVPHDSTQGKIITIRNINKAQSNGSGNGSDSEMDTSSLERGRKVSAVSKPVLYRTRCTRLGRFASYRTSFSVGSDDELGPIRKKEEDQASQGYKGDNAVIPYETDEDPRRRNILRSLRRNTKKPKPKPRPSITKATWESNYFGVPLTTVVTPEKPIPIFIERCIEYIEATGLSTEGIYRVSGNKSEMESLQRQFDQDHNLDLAEKDFTVNTVAGAMKSFFSELPDPLVPYSMQIDLVEAHKINDREQKLHALKEVLKKFPKENHEVFKYVISHLNKVSHNNKVNLMTSENLSICFWPTLMRPDFSSMDALTATRSYQTIIELFIQQCPFFFYNRPISEPPGAAPGSPSAMAPTVPFLTSTPATSQPSPPQSPPPTPQSPMQPLLSSQLQAEHTL

Target ORF information:

RefSeq Version	XM_006539809.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho GTPase activating protein 35 (Arhgap35), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006539809.4

ORF Insert Sequence:

ATGATGATGG CAAGAAAGCA AGATGTCCGC ATCCCCACCT ACAACATCAG TGTGGTGGGA 
TTGTCAGGCA CTGAGAAGGA GAAAGGCCAG TGCGGCATTG GGAAGTCTTG TCTGTGTAAC 
CGCTTTGTGC GCCCAAGTGC TGATGAGTTT CACCTGGACC ACACTTCTGT CCTTAGCACC 
AGTGACTTTG GTGGGCGGGT GGTCAATAAT GACCACTTTC TGTACTGGGG AGAAGTTAGC 
CGCTCCCTGG AGGATTGTGT GGAATGTAAG ATGCACATTG TGGAGCAGAC TGAGTTTATT 
GACGATCAGA CTTTTCAACC TCATCGAAGC ACGGCACTGC AGCCCTACAT CAAGAGAGCC 
GCAGCCACCA AGCTTGCTTC AGCTGAAAAA CTCATGTATT TTTGCACTGA CCAGCTGGGA 
CTGGAGCAAG ACTTTGAGCA GAAACAGATG CCAGATGGGA AGCTGCTGGT TGACGGTTTC 
CTTCTGGGCA TTGATGTTAG CAGGGGCATG AACAGGAACT TCGATGATCA GCTCAAATTT 
GTCTCCAATC TCTACAATCA GCTTGCAAAA ACAAAAAAGC CCATAGTGGT AGTCCTGACT 
AAGTGTGATG AGGGCGTTGA GCGGTACATT AGAGATGCAC ATACTTTTGC CTTAAGCAAA 
AAGAACCTCC AGGTTGTAGA GACCTCTGCA AGGTCCAATG TGAATGTGGA CTTGGCTTTC 
AGCACCTTAG TGCAACTCAT TGATAAGAGT CGAGGGAAGA CAAAAATCAT CCCTTACTTT 
GAAGCTCTCA AGCAGCAGAG TCAGCAGATA GCTACAGCAA AGGACAAGTA TGAGTGGTTA 
GTGAGCCGCA TTGTGAAAAA TCACAATGAG AACTGGCCGA GTGTCAGCCG AAAGATGCAG 
GCCTCCCCTG AGTACCAGGA CTATGTCTAC CTGGAAGGGA CACAGAAGGC CAAGAAGCTC 
TTCCTGCAGC ACATTCACCG CCTCAAGCAT GAGCATATTG AGCGTCGGAG AAAGCTGTAC 
CTGGCAGCTC TGCCATTGGC TTTTGAAGCC CTCATACCTA ATCTAGATGA AGTGGACCAC 
CTGAGCTGTA TTAAAGCAAA AAAGCTGTTA GAGACCAAGC CAGAGTTCTT AAAGTGGTTT 
GTTGTACTTG AAGAAACACC ATGGGATGCC ACCAGCCACA TTGACAACAT GGAAAATGAG 
CGGATTCCCT TTGACTTGAT GGATACTGTC CCTGCTGAGC AGTTGTATGA GACCCACTTG 
GAGAAGCTGA GGAACGAGAG GAAGAGAGCT GAGATGCGAA GGGCTTTCAA AGAAAACCTG 
GAGACCTCTC CTTTCATAAC TCCTGGGAAA CCTTGGGAAG AAGCTCGTAG TTTTATTATG 
AATGAAGACT TCTACCAGTG GCTGGAAGAA TCTGTGTACA TGGACATCTA TGGCAAGCAC 
CAAAAGCAGA TTATAGACCG AGCAAAGGAA GAGTTCCAAG AGTTGCTTTT GGAGTACTCA 
GAATTGTTTT ATGAGCTGGA GCTGGACGCT AAGCCCAGCA AGGAGAAGAT GGGTGTCATC 
CAGGATGTTC TGGGCGAAGA GCAGAGATTT AAAGCATTGC AGAAGCTCCA AGCAGAGCGT 
GATGCCCTCA TTCTGAAGCA CATCCATTTT GTGTACCACC CGACGAAGGA GACATGCCCA 
AGCTGTCCAG CTTGTGTAGA TGCTAAGATT GAACACTTGA TCAGTTCTCG CTTTATCAGA 
CCATCTGATC GGAATCAGAA GAACTCTCTG TCTGACCTCA ATATTGATAG GATCAATTTG 
GTTATCTTAG GCAAAGATGG CCTTGCCCGA GAGTTGGCCA ATGAAATTCG AGCTCTTTGT 
ACAAATGATG ACAAGTATGT AATAGATGGT AAAATGTATG AGCTTTCTCT GAGGCCAATA 
GAGGGGAATG TCCGGCTTCC TGTGAACTCT TTCCAGACAC CAACCTTCCA ACCCCATGGC 
TGTCTCTGCC TTTACAATTC AAAGGAGTCA CTGTCCTATG TGGTGGAGAG TATAGAGAAG 
AGCAGAGAGT CTACACTGGG CAGGCGGGAT AATCATTTAG TCCACCTTCC CTTGACCTTA 
ATTTTAGTTA ACAAGAGAGG GGACACAAGT GGAGAGACTC TGCACAGCTT GATACAGCAA 
GGTCAGCAGA TTGCCAGCAA ACTTCAGTGT GTCTTTCTTG ATCCGGCTTC TGCTGGCATT 
GGTTATGGAC GCAACATTAA TGAGAAGCAG ATCAGTCAAG TTCTGAAAGG ACTCCTGGAC 
TCCAAGCGCA ACTTAAACCT GGTTAGCTCC ACTGCAAGTA TCAAAGATTT GGCTGATGTG 
GACCTTCGAA TTGTCATGTG TCTGATGTGT GGTGATCCCT TTAGTGCAGA TGACGTTCTC 
TCTCCTGTCC TGCAATCCCA AACTTGTAAA TCTTCCCACT GTGGGAGCAG CAACTCTGTT 
TTACTTGAAC TTCCAATTGG ACTACACAAG AAGCGCATTG AGCTGTCTGT TCTTTCATAC 
CATTCCTCAT TTAGCATCCG AAAGAGCCGG TTGGTTCATG GGTACATTGT TTTTTATTCA 
GCCAAACGTA AGGCCTCCTT GGCAATGTTA CGTGCCTTTC TTTGTGAAGT GCAGGATATT 
ATCCCCATCC AGCTTGTCGC ACTCACTGAT GGCGCTATAG ATGTCCTAGA CAATGACTTA 
AGTCGAGAGC AGCTAACAGA GGGAGAGGAA ATTGCACAAG AGATTGACGG GAGATTCACA 
AGCATCCCTT GTAGCCAGCC CCAGCATAAA CTTGAGCTCT TTCATCCCTT TTTTAAAGAT 
GTGGTGGAGA AAAAGAACAT AATTGAGGCC ACACATATGT ATGATAATGT AGCTGAGGCC 
TGCAGCACCA CTGAGGAAGT GTTCAACTCC CCCAGAGCTG GCTCACCCCT CTGCAACTCA 
AACTTACAGG ACTCAGAAGA AGATGTGGAG CCTCCATCAT ACCACCTGTT TCGGGAAGAT 
GCGACATTGC CCTCCCTGTC CAAAGATCAT TCCAAGTTCT CTATGGAACT GGAGGGAAAT 
GACGGGCTAT CTTTCATAAT GAGCAATTTT GAGAGTAAAC TGAACAACAA AGTACCTCCC 
CCAGTCAAAC CAAAGCCTCC TGTGCATTTT GATATCACAA AAGATCTTTC TTACTTAGAC 
CAAGGCCATC GGGAGGGACA GAGGAAATCT ATGTCTTCTA GCCCCTGGAT GCCTCAGGAT 
GGGTTTGATC CTTCTGACTA CGCAGAGCCC ATGGATGCTG TGGTCAAGCC AAGGAATGAG 
GAAGAAAACA TATACTCGGT GCCCCACGAC AGCACCCAGG GCAAGATCAT TACCATTCGG 
AACATCAACA AAGCCCAGTC CAATGGCAGT GGTAATGGTT CTGACAGTGA GATGGACACA 
AGTTCCCTAG AGCGAGGCCG CAAGGTATCT GCAGTGAGTA AGCCTGTGCT GTACAGGACA 
AGATGCACTC GCCTGGGGCG GTTTGCTAGT TACCGTACCA GCTTCAGTGT CGGGAGTGAT 
GATGAGCTGG GACCCATCCG AAAGAAAGAG GAGGACCAGG CATCCCAAGG TTATAAAGGG 
GACAATGCTG TCATTCCATA TGAAACAGAT GAGGACCCCA GGAGGAGGAA TATCCTTCGA 
AGTCTAAGAA GGAACACCAA GAAACCAAAG CCCAAACCCC GGCCATCCAT CACAAAGGCA 
ACCTGGGAGA GTAACTATTT TGGGGTGCCC TTAACAACAG TGGTGACTCC AGAGAAACCG 
ATACCCATTT TCATTGAAAG ATGCATTGAG TACATCGAAG CCACAGGACT AAGCACTGAA 
GGCATCTACC GGGTCAGTGG AAACAAGTCA GAAATGGAAA GTTTGCAAAG ACAGTTTGAT 
CAAGACCACA ATCTGGACCT GGCAGAGAAA GACTTCACTG TGAACACTGT GGCAGGGGCC 
ATGAAGAGTT TTTTCTCAGA GCTACCAGAC CCCCTGGTAC CATACAGCAT GCAGATTGAC 
TTGGTGGAAG CTCACAAAAT CAACGACAGG GAACAGAAGC TGCATGCTCT GAAGGAAGTG 
CTGAAGAAGT TCCCGAAGGA AAACCATGAA GTCTTCAAAT ATGTCATCTC CCACCTGAAC 
AAAGTCAGCC ACAACAACAA GGTGAATCTT ATGACCAGTG AGAACCTGTC CATCTGCTTC 
TGGCCCACGC TGATGCGGCC TGACTTCAGC AGCATGGACG CGCTCACAGC CACGCGCTCC 
TACCAGACCA TCATCGAGCT CTTCATCCAG CAGTGCCCCT TCTTCTTCTA CAACCGGCCC 
ATCAGCGAGC CTCCAGGGGC TGCGCCTGGC TCCCCTTCAG CCATGGCTCC CACTGTTCCC 
TTCCTCACCT CTACACCTGC TACCAGTCAG CCATCACCTC CCCAGTCACC TCCTCCAACC 
CCTCAGTCCC CAATGCAGCC ATTGCTGTCC TCTCAGCTCC AAGCCGAACA CACGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgap35 ( NM_172739.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_766327.3 Mus musculus Rho GTPase activating protein 35 (Arhgap35), mRNA.

Price & Availability↑

CloneID	OMu20691
Clone ID Related Accession (Same CDS sequence)	XM_006539809.3 , XM_011250512.2 , XM_011250512.3 , XM_006539809.4 , NM_172739.4
Accession Version	NM_172739.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	rho GTPase-activating protein 35
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK147326.1, AK136766.1, AK032559.1 and AK137250.1. On Sep 14, 2006 this sequence version replaced NM_172739.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK147326.1, AK147688.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849382, SAMN01164131 [ECO:0000348] ##Evidence-Data-END##

ATGATGATGG CAAGAAAGCA AGATGTCCGC ATCCCCACCT ACAACATCAG TGTGGTGGGA 
TTGTCAGGCA CTGAGAAGGA GAAAGGCCAG TGCGGCATTG GGAAGTCTTG TCTGTGTAAC 
CGCTTTGTGC GCCCAAGTGC TGATGAGTTT CACCTGGACC ACACTTCTGT CCTTAGCACC 
AGTGACTTTG GTGGGCGGGT GGTCAATAAT GACCACTTTC TGTACTGGGG AGAAGTTAGC 
CGCTCCCTGG AGGATTGTGT GGAATGTAAG ATGCACATTG TGGAGCAGAC TGAGTTTATT 
GACGATCAGA CTTTTCAACC TCATCGAAGC ACGGCACTGC AGCCCTACAT CAAGAGAGCC 
GCAGCCACCA AGCTTGCTTC AGCTGAAAAA CTCATGTATT TTTGCACTGA CCAGCTGGGA 
CTGGAGCAAG ACTTTGAGCA GAAACAGATG CCAGATGGGA AGCTGCTGGT TGACGGTTTC 
CTTCTGGGCA TTGATGTTAG CAGGGGCATG AACAGGAACT TCGATGATCA GCTCAAATTT 
GTCTCCAATC TCTACAATCA GCTTGCAAAA ACAAAAAAGC CCATAGTGGT AGTCCTGACT 
AAGTGTGATG AGGGCGTTGA GCGGTACATT AGAGATGCAC ATACTTTTGC CTTAAGCAAA 
AAGAACCTCC AGGTTGTAGA GACCTCTGCA AGGTCCAATG TGAATGTGGA CTTGGCTTTC 
AGCACCTTAG TGCAACTCAT TGATAAGAGT CGAGGGAAGA CAAAAATCAT CCCTTACTTT 
GAAGCTCTCA AGCAGCAGAG TCAGCAGATA GCTACAGCAA AGGACAAGTA TGAGTGGTTA 
GTGAGCCGCA TTGTGAAAAA TCACAATGAG AACTGGCCGA GTGTCAGCCG AAAGATGCAG 
GCCTCCCCTG AGTACCAGGA CTATGTCTAC CTGGAAGGGA CACAGAAGGC CAAGAAGCTC 
TTCCTGCAGC ACATTCACCG CCTCAAGCAT GAGCATATTG AGCGTCGGAG AAAGCTGTAC 
CTGGCAGCTC TGCCATTGGC TTTTGAAGCC CTCATACCTA ATCTAGATGA AGTGGACCAC 
CTGAGCTGTA TTAAAGCAAA AAAGCTGTTA GAGACCAAGC CAGAGTTCTT AAAGTGGTTT 
GTTGTACTTG AAGAAACACC ATGGGATGCC ACCAGCCACA TTGACAACAT GGAAAATGAG 
CGGATTCCCT TTGACTTGAT GGATACTGTC CCTGCTGAGC AGTTGTATGA GACCCACTTG 
GAGAAGCTGA GGAACGAGAG GAAGAGAGCT GAGATGCGAA GGGCTTTCAA AGAAAACCTG 
GAGACCTCTC CTTTCATAAC TCCTGGGAAA CCTTGGGAAG AAGCTCGTAG TTTTATTATG 
AATGAAGACT TCTACCAGTG GCTGGAAGAA TCTGTGTACA TGGACATCTA TGGCAAGCAC 
CAAAAGCAGA TTATAGACCG AGCAAAGGAA GAGTTCCAAG AGTTGCTTTT GGAGTACTCA 
GAATTGTTTT ATGAGCTGGA GCTGGACGCT AAGCCCAGCA AGGAGAAGAT GGGTGTCATC 
CAGGATGTTC TGGGCGAAGA GCAGAGATTT AAAGCATTGC AGAAGCTCCA AGCAGAGCGT 
GATGCCCTCA TTCTGAAGCA CATCCATTTT GTGTACCACC CGACGAAGGA GACATGCCCA 
AGCTGTCCAG CTTGTGTAGA TGCTAAGATT GAACACTTGA TCAGTTCTCG CTTTATCAGA 
CCATCTGATC GGAATCAGAA GAACTCTCTG TCTGACCTCA ATATTGATAG GATCAATTTG 
GTTATCTTAG GCAAAGATGG CCTTGCCCGA GAGTTGGCCA ATGAAATTCG AGCTCTTTGT 
ACAAATGATG ACAAGTATGT AATAGATGGT AAAATGTATG AGCTTTCTCT GAGGCCAATA 
GAGGGGAATG TCCGGCTTCC TGTGAACTCT TTCCAGACAC CAACCTTCCA ACCCCATGGC 
TGTCTCTGCC TTTACAATTC AAAGGAGTCA CTGTCCTATG TGGTGGAGAG TATAGAGAAG 
AGCAGAGAGT CTACACTGGG CAGGCGGGAT AATCATTTAG TCCACCTTCC CTTGACCTTA 
ATTTTAGTTA ACAAGAGAGG GGACACAAGT GGAGAGACTC TGCACAGCTT GATACAGCAA 
GGTCAGCAGA TTGCCAGCAA ACTTCAGTGT GTCTTTCTTG ATCCGGCTTC TGCTGGCATT 
GGTTATGGAC GCAACATTAA TGAGAAGCAG ATCAGTCAAG TTCTGAAAGG ACTCCTGGAC 
TCCAAGCGCA ACTTAAACCT GGTTAGCTCC ACTGCAAGTA TCAAAGATTT GGCTGATGTG 
GACCTTCGAA TTGTCATGTG TCTGATGTGT GGTGATCCCT TTAGTGCAGA TGACGTTCTC 
TCTCCTGTCC TGCAATCCCA AACTTGTAAA TCTTCCCACT GTGGGAGCAG CAACTCTGTT 
TTACTTGAAC TTCCAATTGG ACTACACAAG AAGCGCATTG AGCTGTCTGT TCTTTCATAC 
CATTCCTCAT TTAGCATCCG AAAGAGCCGG TTGGTTCATG GGTACATTGT TTTTTATTCA 
GCCAAACGTA AGGCCTCCTT GGCAATGTTA CGTGCCTTTC TTTGTGAAGT GCAGGATATT 
ATCCCCATCC AGCTTGTCGC ACTCACTGAT GGCGCTATAG ATGTCCTAGA CAATGACTTA 
AGTCGAGAGC AGCTAACAGA GGGAGAGGAA ATTGCACAAG AGATTGACGG GAGATTCACA 
AGCATCCCTT GTAGCCAGCC CCAGCATAAA CTTGAGCTCT TTCATCCCTT TTTTAAAGAT 
GTGGTGGAGA AAAAGAACAT AATTGAGGCC ACACATATGT ATGATAATGT AGCTGAGGCC 
TGCAGCACCA CTGAGGAAGT GTTCAACTCC CCCAGAGCTG GCTCACCCCT CTGCAACTCA 
AACTTACAGG ACTCAGAAGA AGATGTGGAG CCTCCATCAT ACCACCTGTT TCGGGAAGAT 
GCGACATTGC CCTCCCTGTC CAAAGATCAT TCCAAGTTCT CTATGGAACT GGAGGGAAAT 
GACGGGCTAT CTTTCATAAT GAGCAATTTT GAGAGTAAAC TGAACAACAA AGTACCTCCC 
CCAGTCAAAC CAAAGCCTCC TGTGCATTTT GATATCACAA AAGATCTTTC TTACTTAGAC 
CAAGGCCATC GGGAGGGACA GAGGAAATCT ATGTCTTCTA GCCCCTGGAT GCCTCAGGAT 
GGGTTTGATC CTTCTGACTA CGCAGAGCCC ATGGATGCTG TGGTCAAGCC AAGGAATGAG 
GAAGAAAACA TATACTCGGT GCCCCACGAC AGCACCCAGG GCAAGATCAT TACCATTCGG 
AACATCAACA AAGCCCAGTC CAATGGCAGT GGTAATGGTT CTGACAGTGA GATGGACACA 
AGTTCCCTAG AGCGAGGCCG CAAGGTATCT GCAGTGAGTA AGCCTGTGCT GTACAGGACA 
AGATGCACTC GCCTGGGGCG GTTTGCTAGT TACCGTACCA GCTTCAGTGT CGGGAGTGAT 
GATGAGCTGG GACCCATCCG AAAGAAAGAG GAGGACCAGG CATCCCAAGG TTATAAAGGG 
GACAATGCTG TCATTCCATA TGAAACAGAT GAGGACCCCA GGAGGAGGAA TATCCTTCGA 
AGTCTAAGAA GGAACACCAA GAAACCAAAG CCCAAACCCC GGCCATCCAT CACAAAGGCA 
ACCTGGGAGA GTAACTATTT TGGGGTGCCC TTAACAACAG TGGTGACTCC AGAGAAACCG 
ATACCCATTT TCATTGAAAG ATGCATTGAG TACATCGAAG CCACAGGACT AAGCACTGAA 
GGCATCTACC GGGTCAGTGG AAACAAGTCA GAAATGGAAA GTTTGCAAAG ACAGTTTGAT 
CAAGACCACA ATCTGGACCT GGCAGAGAAA GACTTCACTG TGAACACTGT GGCAGGGGCC 
ATGAAGAGTT TTTTCTCAGA GCTACCAGAC CCCCTGGTAC CATACAGCAT GCAGATTGAC 
TTGGTGGAAG CTCACAAAAT CAACGACAGG GAACAGAAGC TGCATGCTCT GAAGGAAGTG 
CTGAAGAAGT TCCCGAAGGA AAACCATGAA GTCTTCAAAT ATGTCATCTC CCACCTGAAC 
AAAGTCAGCC ACAACAACAA GGTGAATCTT ATGACCAGTG AGAACCTGTC CATCTGCTTC 
TGGCCCACGC TGATGCGGCC TGACTTCAGC AGCATGGACG CGCTCACAGC CACGCGCTCC 
TACCAGACCA TCATCGAGCT CTTCATCCAG CAGTGCCCCT TCTTCTTCTA CAACCGGCCC 
ATCAGCGAGC CTCCAGGGGC TGCGCCTGGC TCCCCTTCAG CCATGGCTCC CACTGTTCCC 
TTCCTCACCT CTACACCTGC TACCAGTCAG CCATCACCTC CCCAGTCACC TCCTCCAACC 
CCTCAGTCCC CAATGCAGCC ATTGCTGTCC TCTCAGCTCC AAGCCGAACA CACGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_766327.3
CDS	714..5213
Translation	MMMARKQDVRIPTYNISVVGLSGTEKEKGQCGIGKSCLCNRFVRPSADEFHLDHTSVLSTSDFGGRVVNNDHFLYWGEVSRSLEDCVECKMHIVEQTEFIDDQTFQPHRSTALQPYIKRAAATKLASAEKLMYFCTDQLGLEQDFEQKQMPDGKLLVDGFLLGIDVSRGMNRNFDDQLKFVSNLYNQLAKTKKPIVVVLTKCDEGVERYIRDAHTFALSKKNLQVVETSARSNVNVDLAFSTLVQLIDKSRGKTKIIPYFEALKQQSQQIATAKDKYEWLVSRIVKNHNENWPSVSRKMQASPEYQDYVYLEGTQKAKKLFLQHIHRLKHEHIERRRKLYLAALPLAFEALIPNLDEVDHLSCIKAKKLLETKPEFLKWFVVLEETPWDATSHIDNMENERIPFDLMDTVPAEQLYETHLEKLRNERKRAEMRRAFKENLETSPFITPGKPWEEARSFIMNEDFYQWLEESVYMDIYGKHQKQIIDRAKEEFQELLLEYSELFYELELDAKPSKEKMGVIQDVLGEEQRFKALQKLQAERDALILKHIHFVYHPTKETCPSCPACVDAKIEHLISSRFIRPSDRNQKNSLSDLNIDRINLVILGKDGLARELANEIRALCTNDDKYVIDGKMYELSLRPIEGNVRLPVNSFQTPTFQPHGCLCLYNSKESLSYVVESIEKSRESTLGRRDNHLVHLPLTLILVNKRGDTSGETLHSLIQQGQQIASKLQCVFLDPASAGIGYGRNINEKQISQVLKGLLDSKRNLNLVSSTASIKDLADVDLRIVMCLMCGDPFSADDVLSPVLQSQTCKSSHCGSSNSVLLELPIGLHKKRIELSVLSYHSSFSIRKSRLVHGYIVFYSAKRKASLAMLRAFLCEVQDIIPIQLVALTDGAIDVLDNDLSREQLTEGEEIAQEIDGRFTSIPCSQPQHKLELFHPFFKDVVEKKNIIEATHMYDNVAEACSTTEEVFNSPRAGSPLCNSNLQDSEEDVEPPSYHLFREDATLPSLSKDHSKFSMELEGNDGLSFIMSNFESKLNNKVPPPVKPKPPVHFDITKDLSYLDQGHREGQRKSMSSSPWMPQDGFDPSDYAEPMDAVVKPRNEEENIYSVPHDSTQGKIITIRNINKAQSNGSGNGSDSEMDTSSLERGRKVSAVSKPVLYRTRCTRLGRFASYRTSFSVGSDDELGPIRKKEEDQASQGYKGDNAVIPYETDEDPRRRNILRSLRRNTKKPKPKPRPSITKATWESNYFGVPLTTVVTPEKPIPIFIERCIEYIEATGLSTEGIYRVSGNKSEMESLQRQFDQDHNLDLAEKDFTVNTVAGAMKSFFSELPDPLVPYSMQIDLVEAHKINDREQKLHALKEVLKKFPKENHEVFKYVISHLNKVSHNNKVNLMTSENLSICFWPTLMRPDFSSMDALTATRSYQTIIELFIQQCPFFFYNRPISEPPGAAPGSPSAMAPTVPFLTSTPATSQPSPPQSPPPTPQSPMQPLLSSQLQAEHTL

Target ORF information:

RefSeq Version	NM_172739.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho GTPase activating protein 35 (Arhgap35), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172739.4

ORF Insert Sequence:

ATGATGATGG CAAGAAAGCA AGATGTCCGC ATCCCCACCT ACAACATCAG TGTGGTGGGA 
TTGTCAGGCA CTGAGAAGGA GAAAGGCCAG TGCGGCATTG GGAAGTCTTG TCTGTGTAAC 
CGCTTTGTGC GCCCAAGTGC TGATGAGTTT CACCTGGACC ACACTTCTGT CCTTAGCACC 
AGTGACTTTG GTGGGCGGGT GGTCAATAAT GACCACTTTC TGTACTGGGG AGAAGTTAGC 
CGCTCCCTGG AGGATTGTGT GGAATGTAAG ATGCACATTG TGGAGCAGAC TGAGTTTATT 
GACGATCAGA CTTTTCAACC TCATCGAAGC ACGGCACTGC AGCCCTACAT CAAGAGAGCC 
GCAGCCACCA AGCTTGCTTC AGCTGAAAAA CTCATGTATT TTTGCACTGA CCAGCTGGGA 
CTGGAGCAAG ACTTTGAGCA GAAACAGATG CCAGATGGGA AGCTGCTGGT TGACGGTTTC 
CTTCTGGGCA TTGATGTTAG CAGGGGCATG AACAGGAACT TCGATGATCA GCTCAAATTT 
GTCTCCAATC TCTACAATCA GCTTGCAAAA ACAAAAAAGC CCATAGTGGT AGTCCTGACT 
AAGTGTGATG AGGGCGTTGA GCGGTACATT AGAGATGCAC ATACTTTTGC CTTAAGCAAA 
AAGAACCTCC AGGTTGTAGA GACCTCTGCA AGGTCCAATG TGAATGTGGA CTTGGCTTTC 
AGCACCTTAG TGCAACTCAT TGATAAGAGT CGAGGGAAGA CAAAAATCAT CCCTTACTTT 
GAAGCTCTCA AGCAGCAGAG TCAGCAGATA GCTACAGCAA AGGACAAGTA TGAGTGGTTA 
GTGAGCCGCA TTGTGAAAAA TCACAATGAG AACTGGCCGA GTGTCAGCCG AAAGATGCAG 
GCCTCCCCTG AGTACCAGGA CTATGTCTAC CTGGAAGGGA CACAGAAGGC CAAGAAGCTC 
TTCCTGCAGC ACATTCACCG CCTCAAGCAT GAGCATATTG AGCGTCGGAG AAAGCTGTAC 
CTGGCAGCTC TGCCATTGGC TTTTGAAGCC CTCATACCTA ATCTAGATGA AGTGGACCAC 
CTGAGCTGTA TTAAAGCAAA AAAGCTGTTA GAGACCAAGC CAGAGTTCTT AAAGTGGTTT 
GTTGTACTTG AAGAAACACC ATGGGATGCC ACCAGCCACA TTGACAACAT GGAAAATGAG 
CGGATTCCCT TTGACTTGAT GGATACTGTC CCTGCTGAGC AGTTGTATGA GACCCACTTG 
GAGAAGCTGA GGAACGAGAG GAAGAGAGCT GAGATGCGAA GGGCTTTCAA AGAAAACCTG 
GAGACCTCTC CTTTCATAAC TCCTGGGAAA CCTTGGGAAG AAGCTCGTAG TTTTATTATG 
AATGAAGACT TCTACCAGTG GCTGGAAGAA TCTGTGTACA TGGACATCTA TGGCAAGCAC 
CAAAAGCAGA TTATAGACCG AGCAAAGGAA GAGTTCCAAG AGTTGCTTTT GGAGTACTCA 
GAATTGTTTT ATGAGCTGGA GCTGGACGCT AAGCCCAGCA AGGAGAAGAT GGGTGTCATC 
CAGGATGTTC TGGGCGAAGA GCAGAGATTT AAAGCATTGC AGAAGCTCCA AGCAGAGCGT 
GATGCCCTCA TTCTGAAGCA CATCCATTTT GTGTACCACC CGACGAAGGA GACATGCCCA 
AGCTGTCCAG CTTGTGTAGA TGCTAAGATT GAACACTTGA TCAGTTCTCG CTTTATCAGA 
CCATCTGATC GGAATCAGAA GAACTCTCTG TCTGACCTCA ATATTGATAG GATCAATTTG 
GTTATCTTAG GCAAAGATGG CCTTGCCCGA GAGTTGGCCA ATGAAATTCG AGCTCTTTGT 
ACAAATGATG ACAAGTATGT AATAGATGGT AAAATGTATG AGCTTTCTCT GAGGCCAATA 
GAGGGGAATG TCCGGCTTCC TGTGAACTCT TTCCAGACAC CAACCTTCCA ACCCCATGGC 
TGTCTCTGCC TTTACAATTC AAAGGAGTCA CTGTCCTATG TGGTGGAGAG TATAGAGAAG 
AGCAGAGAGT CTACACTGGG CAGGCGGGAT AATCATTTAG TCCACCTTCC CTTGACCTTA 
ATTTTAGTTA ACAAGAGAGG GGACACAAGT GGAGAGACTC TGCACAGCTT GATACAGCAA 
GGTCAGCAGA TTGCCAGCAA ACTTCAGTGT GTCTTTCTTG ATCCGGCTTC TGCTGGCATT 
GGTTATGGAC GCAACATTAA TGAGAAGCAG ATCAGTCAAG TTCTGAAAGG ACTCCTGGAC 
TCCAAGCGCA ACTTAAACCT GGTTAGCTCC ACTGCAAGTA TCAAAGATTT GGCTGATGTG 
GACCTTCGAA TTGTCATGTG TCTGATGTGT GGTGATCCCT TTAGTGCAGA TGACGTTCTC 
TCTCCTGTCC TGCAATCCCA AACTTGTAAA TCTTCCCACT GTGGGAGCAG CAACTCTGTT 
TTACTTGAAC TTCCAATTGG ACTACACAAG AAGCGCATTG AGCTGTCTGT TCTTTCATAC 
CATTCCTCAT TTAGCATCCG AAAGAGCCGG TTGGTTCATG GGTACATTGT TTTTTATTCA 
GCCAAACGTA AGGCCTCCTT GGCAATGTTA CGTGCCTTTC TTTGTGAAGT GCAGGATATT 
ATCCCCATCC AGCTTGTCGC ACTCACTGAT GGCGCTATAG ATGTCCTAGA CAATGACTTA 
AGTCGAGAGC AGCTAACAGA GGGAGAGGAA ATTGCACAAG AGATTGACGG GAGATTCACA 
AGCATCCCTT GTAGCCAGCC CCAGCATAAA CTTGAGCTCT TTCATCCCTT TTTTAAAGAT 
GTGGTGGAGA AAAAGAACAT AATTGAGGCC ACACATATGT ATGATAATGT AGCTGAGGCC 
TGCAGCACCA CTGAGGAAGT GTTCAACTCC CCCAGAGCTG GCTCACCCCT CTGCAACTCA 
AACTTACAGG ACTCAGAAGA AGATGTGGAG CCTCCATCAT ACCACCTGTT TCGGGAAGAT 
GCGACATTGC CCTCCCTGTC CAAAGATCAT TCCAAGTTCT CTATGGAACT GGAGGGAAAT 
GACGGGCTAT CTTTCATAAT GAGCAATTTT GAGAGTAAAC TGAACAACAA AGTACCTCCC 
CCAGTCAAAC CAAAGCCTCC TGTGCATTTT GATATCACAA AAGATCTTTC TTACTTAGAC 
CAAGGCCATC GGGAGGGACA GAGGAAATCT ATGTCTTCTA GCCCCTGGAT GCCTCAGGAT 
GGGTTTGATC CTTCTGACTA CGCAGAGCCC ATGGATGCTG TGGTCAAGCC AAGGAATGAG 
GAAGAAAACA TATACTCGGT GCCCCACGAC AGCACCCAGG GCAAGATCAT TACCATTCGG 
AACATCAACA AAGCCCAGTC CAATGGCAGT GGTAATGGTT CTGACAGTGA GATGGACACA 
AGTTCCCTAG AGCGAGGCCG CAAGGTATCT GCAGTGAGTA AGCCTGTGCT GTACAGGACA 
AGATGCACTC GCCTGGGGCG GTTTGCTAGT TACCGTACCA GCTTCAGTGT CGGGAGTGAT 
GATGAGCTGG GACCCATCCG AAAGAAAGAG GAGGACCAGG CATCCCAAGG TTATAAAGGG 
GACAATGCTG TCATTCCATA TGAAACAGAT GAGGACCCCA GGAGGAGGAA TATCCTTCGA 
AGTCTAAGAA GGAACACCAA GAAACCAAAG CCCAAACCCC GGCCATCCAT CACAAAGGCA 
ACCTGGGAGA GTAACTATTT TGGGGTGCCC TTAACAACAG TGGTGACTCC AGAGAAACCG 
ATACCCATTT TCATTGAAAG ATGCATTGAG TACATCGAAG CCACAGGACT AAGCACTGAA 
GGCATCTACC GGGTCAGTGG AAACAAGTCA GAAATGGAAA GTTTGCAAAG ACAGTTTGAT 
CAAGACCACA ATCTGGACCT GGCAGAGAAA GACTTCACTG TGAACACTGT GGCAGGGGCC 
ATGAAGAGTT TTTTCTCAGA GCTACCAGAC CCCCTGGTAC CATACAGCAT GCAGATTGAC 
TTGGTGGAAG CTCACAAAAT CAACGACAGG GAACAGAAGC TGCATGCTCT GAAGGAAGTG 
CTGAAGAAGT TCCCGAAGGA AAACCATGAA GTCTTCAAAT ATGTCATCTC CCACCTGAAC 
AAAGTCAGCC ACAACAACAA GGTGAATCTT ATGACCAGTG AGAACCTGTC CATCTGCTTC 
TGGCCCACGC TGATGCGGCC TGACTTCAGC AGCATGGACG CGCTCACAGC CACGCGCTCC 
TACCAGACCA TCATCGAGCT CTTCATCCAG CAGTGCCCCT TCTTCTTCTA CAACCGGCCC 
ATCAGCGAGC CTCCAGGGGC TGCGCCTGGC TCCCCTTCAG CCATGGCTCC CACTGTTCCC 
TTCCTCACCT CTACACCTGC TACCAGTCAG CCATCACCTC CCCAGTCACC TCCTCCAACC 
CCTCAGTCCC CAATGCAGCC ATTGCTGTCC TCTCAGCTCC AAGCCGAACA CACGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgap35 ( XM_006539809.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006539872.1 Mus musculus Rho GTPase activating protein 35 (Arhgap35), transcript variant X2, mRNA.

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CloneID	OMu20691
Clone ID Related Accession (Same CDS sequence)	XM_006539809.3 , XM_011250512.2 , XM_011250512.3 , XM_006539809.4 , NM_172739.4
Accession Version	XM_006539809.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	rho GTPase-activating protein 35 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006539809.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGG CAAGAAAGCA AGATGTCCGC ATCCCCACCT ACAACATCAG TGTGGTGGGA 
TTGTCAGGCA CTGAGAAGGA GAAAGGCCAG TGCGGCATTG GGAAGTCTTG TCTGTGTAAC 
CGCTTTGTGC GCCCAAGTGC TGATGAGTTT CACCTGGACC ACACTTCTGT CCTTAGCACC 
AGTGACTTTG GTGGGCGGGT GGTCAATAAT GACCACTTTC TGTACTGGGG AGAAGTTAGC 
CGCTCCCTGG AGGATTGTGT GGAATGTAAG ATGCACATTG TGGAGCAGAC TGAGTTTATT 
GACGATCAGA CTTTTCAACC TCATCGAAGC ACGGCACTGC AGCCCTACAT CAAGAGAGCC 
GCAGCCACCA AGCTTGCTTC AGCTGAAAAA CTCATGTATT TTTGCACTGA CCAGCTGGGA 
CTGGAGCAAG ACTTTGAGCA GAAACAGATG CCAGATGGGA AGCTGCTGGT TGACGGTTTC 
CTTCTGGGCA TTGATGTTAG CAGGGGCATG AACAGGAACT TCGATGATCA GCTCAAATTT 
GTCTCCAATC TCTACAATCA GCTTGCAAAA ACAAAAAAGC CCATAGTGGT AGTCCTGACT 
AAGTGTGATG AGGGCGTTGA GCGGTACATT AGAGATGCAC ATACTTTTGC CTTAAGCAAA 
AAGAACCTCC AGGTTGTAGA GACCTCTGCA AGGTCCAATG TGAATGTGGA CTTGGCTTTC 
AGCACCTTAG TGCAACTCAT TGATAAGAGT CGAGGGAAGA CAAAAATCAT CCCTTACTTT 
GAAGCTCTCA AGCAGCAGAG TCAGCAGATA GCTACAGCAA AGGACAAGTA TGAGTGGTTA 
GTGAGCCGCA TTGTGAAAAA TCACAATGAG AACTGGCCGA GTGTCAGCCG AAAGATGCAG 
GCCTCCCCTG AGTACCAGGA CTATGTCTAC CTGGAAGGGA CACAGAAGGC CAAGAAGCTC 
TTCCTGCAGC ACATTCACCG CCTCAAGCAT GAGCATATTG AGCGTCGGAG AAAGCTGTAC 
CTGGCAGCTC TGCCATTGGC TTTTGAAGCC CTCATACCTA ATCTAGATGA AGTGGACCAC 
CTGAGCTGTA TTAAAGCAAA AAAGCTGTTA GAGACCAAGC CAGAGTTCTT AAAGTGGTTT 
GTTGTACTTG AAGAAACACC ATGGGATGCC ACCAGCCACA TTGACAACAT GGAAAATGAG 
CGGATTCCCT TTGACTTGAT GGATACTGTC CCTGCTGAGC AGTTGTATGA GACCCACTTG 
GAGAAGCTGA GGAACGAGAG GAAGAGAGCT GAGATGCGAA GGGCTTTCAA AGAAAACCTG 
GAGACCTCTC CTTTCATAAC TCCTGGGAAA CCTTGGGAAG AAGCTCGTAG TTTTATTATG 
AATGAAGACT TCTACCAGTG GCTGGAAGAA TCTGTGTACA TGGACATCTA TGGCAAGCAC 
CAAAAGCAGA TTATAGACCG AGCAAAGGAA GAGTTCCAAG AGTTGCTTTT GGAGTACTCA 
GAATTGTTTT ATGAGCTGGA GCTGGACGCT AAGCCCAGCA AGGAGAAGAT GGGTGTCATC 
CAGGATGTTC TGGGCGAAGA GCAGAGATTT AAAGCATTGC AGAAGCTCCA AGCAGAGCGT 
GATGCCCTCA TTCTGAAGCA CATCCATTTT GTGTACCACC CGACGAAGGA GACATGCCCA 
AGCTGTCCAG CTTGTGTAGA TGCTAAGATT GAACACTTGA TCAGTTCTCG CTTTATCAGA 
CCATCTGATC GGAATCAGAA GAACTCTCTG TCTGACCTCA ATATTGATAG GATCAATTTG 
GTTATCTTAG GCAAAGATGG CCTTGCCCGA GAGTTGGCCA ATGAAATTCG AGCTCTTTGT 
ACAAATGATG ACAAGTATGT AATAGATGGT AAAATGTATG AGCTTTCTCT GAGGCCAATA 
GAGGGGAATG TCCGGCTTCC TGTGAACTCT TTCCAGACAC CAACCTTCCA ACCCCATGGC 
TGTCTCTGCC TTTACAATTC AAAGGAGTCA CTGTCCTATG TGGTGGAGAG TATAGAGAAG 
AGCAGAGAGT CTACACTGGG CAGGCGGGAT AATCATTTAG TCCACCTTCC CTTGACCTTA 
ATTTTAGTTA ACAAGAGAGG GGACACAAGT GGAGAGACTC TGCACAGCTT GATACAGCAA 
GGTCAGCAGA TTGCCAGCAA ACTTCAGTGT GTCTTTCTTG ATCCGGCTTC TGCTGGCATT 
GGTTATGGAC GCAACATTAA TGAGAAGCAG ATCAGTCAAG TTCTGAAAGG ACTCCTGGAC 
TCCAAGCGCA ACTTAAACCT GGTTAGCTCC ACTGCAAGTA TCAAAGATTT GGCTGATGTG 
GACCTTCGAA TTGTCATGTG TCTGATGTGT GGTGATCCCT TTAGTGCAGA TGACGTTCTC 
TCTCCTGTCC TGCAATCCCA AACTTGTAAA TCTTCCCACT GTGGGAGCAG CAACTCTGTT 
TTACTTGAAC TTCCAATTGG ACTACACAAG AAGCGCATTG AGCTGTCTGT TCTTTCATAC 
CATTCCTCAT TTAGCATCCG AAAGAGCCGG TTGGTTCATG GGTACATTGT TTTTTATTCA 
GCCAAACGTA AGGCCTCCTT GGCAATGTTA CGTGCCTTTC TTTGTGAAGT GCAGGATATT 
ATCCCCATCC AGCTTGTCGC ACTCACTGAT GGCGCTATAG ATGTCCTAGA CAATGACTTA 
AGTCGAGAGC AGCTAACAGA GGGAGAGGAA ATTGCACAAG AGATTGACGG GAGATTCACA 
AGCATCCCTT GTAGCCAGCC CCAGCATAAA CTTGAGCTCT TTCATCCCTT TTTTAAAGAT 
GTGGTGGAGA AAAAGAACAT AATTGAGGCC ACACATATGT ATGATAATGT AGCTGAGGCC 
TGCAGCACCA CTGAGGAAGT GTTCAACTCC CCCAGAGCTG GCTCACCCCT CTGCAACTCA 
AACTTACAGG ACTCAGAAGA AGATGTGGAG CCTCCATCAT ACCACCTGTT TCGGGAAGAT 
GCGACATTGC CCTCCCTGTC CAAAGATCAT TCCAAGTTCT CTATGGAACT GGAGGGAAAT 
GACGGGCTAT CTTTCATAAT GAGCAATTTT GAGAGTAAAC TGAACAACAA AGTACCTCCC 
CCAGTCAAAC CAAAGCCTCC TGTGCATTTT GATATCACAA AAGATCTTTC TTACTTAGAC 
CAAGGCCATC GGGAGGGACA GAGGAAATCT ATGTCTTCTA GCCCCTGGAT GCCTCAGGAT 
GGGTTTGATC CTTCTGACTA CGCAGAGCCC ATGGATGCTG TGGTCAAGCC AAGGAATGAG 
GAAGAAAACA TATACTCGGT GCCCCACGAC AGCACCCAGG GCAAGATCAT TACCATTCGG 
AACATCAACA AAGCCCAGTC CAATGGCAGT GGTAATGGTT CTGACAGTGA GATGGACACA 
AGTTCCCTAG AGCGAGGCCG CAAGGTATCT GCAGTGAGTA AGCCTGTGCT GTACAGGACA 
AGATGCACTC GCCTGGGGCG GTTTGCTAGT TACCGTACCA GCTTCAGTGT CGGGAGTGAT 
GATGAGCTGG GACCCATCCG AAAGAAAGAG GAGGACCAGG CATCCCAAGG TTATAAAGGG 
GACAATGCTG TCATTCCATA TGAAACAGAT GAGGACCCCA GGAGGAGGAA TATCCTTCGA 
AGTCTAAGAA GGAACACCAA GAAACCAAAG CCCAAACCCC GGCCATCCAT CACAAAGGCA 
ACCTGGGAGA GTAACTATTT TGGGGTGCCC TTAACAACAG TGGTGACTCC AGAGAAACCG 
ATACCCATTT TCATTGAAAG ATGCATTGAG TACATCGAAG CCACAGGACT AAGCACTGAA 
GGCATCTACC GGGTCAGTGG AAACAAGTCA GAAATGGAAA GTTTGCAAAG ACAGTTTGAT 
CAAGACCACA ATCTGGACCT GGCAGAGAAA GACTTCACTG TGAACACTGT GGCAGGGGCC 
ATGAAGAGTT TTTTCTCAGA GCTACCAGAC CCCCTGGTAC CATACAGCAT GCAGATTGAC 
TTGGTGGAAG CTCACAAAAT CAACGACAGG GAACAGAAGC TGCATGCTCT GAAGGAAGTG 
CTGAAGAAGT TCCCGAAGGA AAACCATGAA GTCTTCAAAT ATGTCATCTC CCACCTGAAC 
AAAGTCAGCC ACAACAACAA GGTGAATCTT ATGACCAGTG AGAACCTGTC CATCTGCTTC 
TGGCCCACGC TGATGCGGCC TGACTTCAGC AGCATGGACG CGCTCACAGC CACGCGCTCC 
TACCAGACCA TCATCGAGCT CTTCATCCAG CAGTGCCCCT TCTTCTTCTA CAACCGGCCC 
ATCAGCGAGC CTCCAGGGGC TGCGCCTGGC TCCCCTTCAG CCATGGCTCC CACTGTTCCC 
TTCCTCACCT CTACACCTGC TACCAGTCAG CCATCACCTC CCCAGTCACC TCCTCCAACC 
CCTCAGTCCC CAATGCAGCC ATTGCTGTCC TCTCAGCTCC AAGCCGAACA CACGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006539872.1
CDS	961..5460
Translation	MMMARKQDVRIPTYNISVVGLSGTEKEKGQCGIGKSCLCNRFVRPSADEFHLDHTSVLSTSDFGGRVVNNDHFLYWGEVSRSLEDCVECKMHIVEQTEFIDDQTFQPHRSTALQPYIKRAAATKLASAEKLMYFCTDQLGLEQDFEQKQMPDGKLLVDGFLLGIDVSRGMNRNFDDQLKFVSNLYNQLAKTKKPIVVVLTKCDEGVERYIRDAHTFALSKKNLQVVETSARSNVNVDLAFSTLVQLIDKSRGKTKIIPYFEALKQQSQQIATAKDKYEWLVSRIVKNHNENWPSVSRKMQASPEYQDYVYLEGTQKAKKLFLQHIHRLKHEHIERRRKLYLAALPLAFEALIPNLDEVDHLSCIKAKKLLETKPEFLKWFVVLEETPWDATSHIDNMENERIPFDLMDTVPAEQLYETHLEKLRNERKRAEMRRAFKENLETSPFITPGKPWEEARSFIMNEDFYQWLEESVYMDIYGKHQKQIIDRAKEEFQELLLEYSELFYELELDAKPSKEKMGVIQDVLGEEQRFKALQKLQAERDALILKHIHFVYHPTKETCPSCPACVDAKIEHLISSRFIRPSDRNQKNSLSDLNIDRINLVILGKDGLARELANEIRALCTNDDKYVIDGKMYELSLRPIEGNVRLPVNSFQTPTFQPHGCLCLYNSKESLSYVVESIEKSRESTLGRRDNHLVHLPLTLILVNKRGDTSGETLHSLIQQGQQIASKLQCVFLDPASAGIGYGRNINEKQISQVLKGLLDSKRNLNLVSSTASIKDLADVDLRIVMCLMCGDPFSADDVLSPVLQSQTCKSSHCGSSNSVLLELPIGLHKKRIELSVLSYHSSFSIRKSRLVHGYIVFYSAKRKASLAMLRAFLCEVQDIIPIQLVALTDGAIDVLDNDLSREQLTEGEEIAQEIDGRFTSIPCSQPQHKLELFHPFFKDVVEKKNIIEATHMYDNVAEACSTTEEVFNSPRAGSPLCNSNLQDSEEDVEPPSYHLFREDATLPSLSKDHSKFSMELEGNDGLSFIMSNFESKLNNKVPPPVKPKPPVHFDITKDLSYLDQGHREGQRKSMSSSPWMPQDGFDPSDYAEPMDAVVKPRNEEENIYSVPHDSTQGKIITIRNINKAQSNGSGNGSDSEMDTSSLERGRKVSAVSKPVLYRTRCTRLGRFASYRTSFSVGSDDELGPIRKKEEDQASQGYKGDNAVIPYETDEDPRRRNILRSLRRNTKKPKPKPRPSITKATWESNYFGVPLTTVVTPEKPIPIFIERCIEYIEATGLSTEGIYRVSGNKSEMESLQRQFDQDHNLDLAEKDFTVNTVAGAMKSFFSELPDPLVPYSMQIDLVEAHKINDREQKLHALKEVLKKFPKENHEVFKYVISHLNKVSHNNKVNLMTSENLSICFWPTLMRPDFSSMDALTATRSYQTIIELFIQQCPFFFYNRPISEPPGAAPGSPSAMAPTVPFLTSTPATSQPSPPQSPPPTPQSPMQPLLSSQLQAEHTL

Target ORF information:

RefSeq Version	XM_006539809.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho GTPase activating protein 35 (Arhgap35), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006539809.3

ORF Insert Sequence:

ATGATGATGG CAAGAAAGCA AGATGTCCGC ATCCCCACCT ACAACATCAG TGTGGTGGGA 
TTGTCAGGCA CTGAGAAGGA GAAAGGCCAG TGCGGCATTG GGAAGTCTTG TCTGTGTAAC 
CGCTTTGTGC GCCCAAGTGC TGATGAGTTT CACCTGGACC ACACTTCTGT CCTTAGCACC 
AGTGACTTTG GTGGGCGGGT GGTCAATAAT GACCACTTTC TGTACTGGGG AGAAGTTAGC 
CGCTCCCTGG AGGATTGTGT GGAATGTAAG ATGCACATTG TGGAGCAGAC TGAGTTTATT 
GACGATCAGA CTTTTCAACC TCATCGAAGC ACGGCACTGC AGCCCTACAT CAAGAGAGCC 
GCAGCCACCA AGCTTGCTTC AGCTGAAAAA CTCATGTATT TTTGCACTGA CCAGCTGGGA 
CTGGAGCAAG ACTTTGAGCA GAAACAGATG CCAGATGGGA AGCTGCTGGT TGACGGTTTC 
CTTCTGGGCA TTGATGTTAG CAGGGGCATG AACAGGAACT TCGATGATCA GCTCAAATTT 
GTCTCCAATC TCTACAATCA GCTTGCAAAA ACAAAAAAGC CCATAGTGGT AGTCCTGACT 
AAGTGTGATG AGGGCGTTGA GCGGTACATT AGAGATGCAC ATACTTTTGC CTTAAGCAAA 
AAGAACCTCC AGGTTGTAGA GACCTCTGCA AGGTCCAATG TGAATGTGGA CTTGGCTTTC 
AGCACCTTAG TGCAACTCAT TGATAAGAGT CGAGGGAAGA CAAAAATCAT CCCTTACTTT 
GAAGCTCTCA AGCAGCAGAG TCAGCAGATA GCTACAGCAA AGGACAAGTA TGAGTGGTTA 
GTGAGCCGCA TTGTGAAAAA TCACAATGAG AACTGGCCGA GTGTCAGCCG AAAGATGCAG 
GCCTCCCCTG AGTACCAGGA CTATGTCTAC CTGGAAGGGA CACAGAAGGC CAAGAAGCTC 
TTCCTGCAGC ACATTCACCG CCTCAAGCAT GAGCATATTG AGCGTCGGAG AAAGCTGTAC 
CTGGCAGCTC TGCCATTGGC TTTTGAAGCC CTCATACCTA ATCTAGATGA AGTGGACCAC 
CTGAGCTGTA TTAAAGCAAA AAAGCTGTTA GAGACCAAGC CAGAGTTCTT AAAGTGGTTT 
GTTGTACTTG AAGAAACACC ATGGGATGCC ACCAGCCACA TTGACAACAT GGAAAATGAG 
CGGATTCCCT TTGACTTGAT GGATACTGTC CCTGCTGAGC AGTTGTATGA GACCCACTTG 
GAGAAGCTGA GGAACGAGAG GAAGAGAGCT GAGATGCGAA GGGCTTTCAA AGAAAACCTG 
GAGACCTCTC CTTTCATAAC TCCTGGGAAA CCTTGGGAAG AAGCTCGTAG TTTTATTATG 
AATGAAGACT TCTACCAGTG GCTGGAAGAA TCTGTGTACA TGGACATCTA TGGCAAGCAC 
CAAAAGCAGA TTATAGACCG AGCAAAGGAA GAGTTCCAAG AGTTGCTTTT GGAGTACTCA 
GAATTGTTTT ATGAGCTGGA GCTGGACGCT AAGCCCAGCA AGGAGAAGAT GGGTGTCATC 
CAGGATGTTC TGGGCGAAGA GCAGAGATTT AAAGCATTGC AGAAGCTCCA AGCAGAGCGT 
GATGCCCTCA TTCTGAAGCA CATCCATTTT GTGTACCACC CGACGAAGGA GACATGCCCA 
AGCTGTCCAG CTTGTGTAGA TGCTAAGATT GAACACTTGA TCAGTTCTCG CTTTATCAGA 
CCATCTGATC GGAATCAGAA GAACTCTCTG TCTGACCTCA ATATTGATAG GATCAATTTG 
GTTATCTTAG GCAAAGATGG CCTTGCCCGA GAGTTGGCCA ATGAAATTCG AGCTCTTTGT 
ACAAATGATG ACAAGTATGT AATAGATGGT AAAATGTATG AGCTTTCTCT GAGGCCAATA 
GAGGGGAATG TCCGGCTTCC TGTGAACTCT TTCCAGACAC CAACCTTCCA ACCCCATGGC 
TGTCTCTGCC TTTACAATTC AAAGGAGTCA CTGTCCTATG TGGTGGAGAG TATAGAGAAG 
AGCAGAGAGT CTACACTGGG CAGGCGGGAT AATCATTTAG TCCACCTTCC CTTGACCTTA 
ATTTTAGTTA ACAAGAGAGG GGACACAAGT GGAGAGACTC TGCACAGCTT GATACAGCAA 
GGTCAGCAGA TTGCCAGCAA ACTTCAGTGT GTCTTTCTTG ATCCGGCTTC TGCTGGCATT 
GGTTATGGAC GCAACATTAA TGAGAAGCAG ATCAGTCAAG TTCTGAAAGG ACTCCTGGAC 
TCCAAGCGCA ACTTAAACCT GGTTAGCTCC ACTGCAAGTA TCAAAGATTT GGCTGATGTG 
GACCTTCGAA TTGTCATGTG TCTGATGTGT GGTGATCCCT TTAGTGCAGA TGACGTTCTC 
TCTCCTGTCC TGCAATCCCA AACTTGTAAA TCTTCCCACT GTGGGAGCAG CAACTCTGTT 
TTACTTGAAC TTCCAATTGG ACTACACAAG AAGCGCATTG AGCTGTCTGT TCTTTCATAC 
CATTCCTCAT TTAGCATCCG AAAGAGCCGG TTGGTTCATG GGTACATTGT TTTTTATTCA 
GCCAAACGTA AGGCCTCCTT GGCAATGTTA CGTGCCTTTC TTTGTGAAGT GCAGGATATT 
ATCCCCATCC AGCTTGTCGC ACTCACTGAT GGCGCTATAG ATGTCCTAGA CAATGACTTA 
AGTCGAGAGC AGCTAACAGA GGGAGAGGAA ATTGCACAAG AGATTGACGG GAGATTCACA 
AGCATCCCTT GTAGCCAGCC CCAGCATAAA CTTGAGCTCT TTCATCCCTT TTTTAAAGAT 
GTGGTGGAGA AAAAGAACAT AATTGAGGCC ACACATATGT ATGATAATGT AGCTGAGGCC 
TGCAGCACCA CTGAGGAAGT GTTCAACTCC CCCAGAGCTG GCTCACCCCT CTGCAACTCA 
AACTTACAGG ACTCAGAAGA AGATGTGGAG CCTCCATCAT ACCACCTGTT TCGGGAAGAT 
GCGACATTGC CCTCCCTGTC CAAAGATCAT TCCAAGTTCT CTATGGAACT GGAGGGAAAT 
GACGGGCTAT CTTTCATAAT GAGCAATTTT GAGAGTAAAC TGAACAACAA AGTACCTCCC 
CCAGTCAAAC CAAAGCCTCC TGTGCATTTT GATATCACAA AAGATCTTTC TTACTTAGAC 
CAAGGCCATC GGGAGGGACA GAGGAAATCT ATGTCTTCTA GCCCCTGGAT GCCTCAGGAT 
GGGTTTGATC CTTCTGACTA CGCAGAGCCC ATGGATGCTG TGGTCAAGCC AAGGAATGAG 
GAAGAAAACA TATACTCGGT GCCCCACGAC AGCACCCAGG GCAAGATCAT TACCATTCGG 
AACATCAACA AAGCCCAGTC CAATGGCAGT GGTAATGGTT CTGACAGTGA GATGGACACA 
AGTTCCCTAG AGCGAGGCCG CAAGGTATCT GCAGTGAGTA AGCCTGTGCT GTACAGGACA 
AGATGCACTC GCCTGGGGCG GTTTGCTAGT TACCGTACCA GCTTCAGTGT CGGGAGTGAT 
GATGAGCTGG GACCCATCCG AAAGAAAGAG GAGGACCAGG CATCCCAAGG TTATAAAGGG 
GACAATGCTG TCATTCCATA TGAAACAGAT GAGGACCCCA GGAGGAGGAA TATCCTTCGA 
AGTCTAAGAA GGAACACCAA GAAACCAAAG CCCAAACCCC GGCCATCCAT CACAAAGGCA 
ACCTGGGAGA GTAACTATTT TGGGGTGCCC TTAACAACAG TGGTGACTCC AGAGAAACCG 
ATACCCATTT TCATTGAAAG ATGCATTGAG TACATCGAAG CCACAGGACT AAGCACTGAA 
GGCATCTACC GGGTCAGTGG AAACAAGTCA GAAATGGAAA GTTTGCAAAG ACAGTTTGAT 
CAAGACCACA ATCTGGACCT GGCAGAGAAA GACTTCACTG TGAACACTGT GGCAGGGGCC 
ATGAAGAGTT TTTTCTCAGA GCTACCAGAC CCCCTGGTAC CATACAGCAT GCAGATTGAC 
TTGGTGGAAG CTCACAAAAT CAACGACAGG GAACAGAAGC TGCATGCTCT GAAGGAAGTG 
CTGAAGAAGT TCCCGAAGGA AAACCATGAA GTCTTCAAAT ATGTCATCTC CCACCTGAAC 
AAAGTCAGCC ACAACAACAA GGTGAATCTT ATGACCAGTG AGAACCTGTC CATCTGCTTC 
TGGCCCACGC TGATGCGGCC TGACTTCAGC AGCATGGACG CGCTCACAGC CACGCGCTCC 
TACCAGACCA TCATCGAGCT CTTCATCCAG CAGTGCCCCT TCTTCTTCTA CAACCGGCCC 
ATCAGCGAGC CTCCAGGGGC TGCGCCTGGC TCCCCTTCAG CCATGGCTCC CACTGTTCCC 
TTCCTCACCT CTACACCTGC TACCAGTCAG CCATCACCTC CCCAGTCACC TCCTCCAACC 
CCTCAGTCCC CAATGCAGCC ATTGCTGTCC TCTCAGCTCC AAGCCGAACA CACGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgap35 ( XM_011250512.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011248814.1 Mus musculus Rho GTPase activating protein 35 (Arhgap35), transcript variant X1, mRNA.

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CloneID	OMu20691
Clone ID Related Accession (Same CDS sequence)	XM_006539809.3 , XM_011250512.2 , XM_011250512.3 , XM_006539809.4 , NM_172739.4
Accession Version	XM_011250512.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	rho GTPase-activating protein 35 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011250512.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGG CAAGAAAGCA AGATGTCCGC ATCCCCACCT ACAACATCAG TGTGGTGGGA 
TTGTCAGGCA CTGAGAAGGA GAAAGGCCAG TGCGGCATTG GGAAGTCTTG TCTGTGTAAC 
CGCTTTGTGC GCCCAAGTGC TGATGAGTTT CACCTGGACC ACACTTCTGT CCTTAGCACC 
AGTGACTTTG GTGGGCGGGT GGTCAATAAT GACCACTTTC TGTACTGGGG AGAAGTTAGC 
CGCTCCCTGG AGGATTGTGT GGAATGTAAG ATGCACATTG TGGAGCAGAC TGAGTTTATT 
GACGATCAGA CTTTTCAACC TCATCGAAGC ACGGCACTGC AGCCCTACAT CAAGAGAGCC 
GCAGCCACCA AGCTTGCTTC AGCTGAAAAA CTCATGTATT TTTGCACTGA CCAGCTGGGA 
CTGGAGCAAG ACTTTGAGCA GAAACAGATG CCAGATGGGA AGCTGCTGGT TGACGGTTTC 
CTTCTGGGCA TTGATGTTAG CAGGGGCATG AACAGGAACT TCGATGATCA GCTCAAATTT 
GTCTCCAATC TCTACAATCA GCTTGCAAAA ACAAAAAAGC CCATAGTGGT AGTCCTGACT 
AAGTGTGATG AGGGCGTTGA GCGGTACATT AGAGATGCAC ATACTTTTGC CTTAAGCAAA 
AAGAACCTCC AGGTTGTAGA GACCTCTGCA AGGTCCAATG TGAATGTGGA CTTGGCTTTC 
AGCACCTTAG TGCAACTCAT TGATAAGAGT CGAGGGAAGA CAAAAATCAT CCCTTACTTT 
GAAGCTCTCA AGCAGCAGAG TCAGCAGATA GCTACAGCAA AGGACAAGTA TGAGTGGTTA 
GTGAGCCGCA TTGTGAAAAA TCACAATGAG AACTGGCCGA GTGTCAGCCG AAAGATGCAG 
GCCTCCCCTG AGTACCAGGA CTATGTCTAC CTGGAAGGGA CACAGAAGGC CAAGAAGCTC 
TTCCTGCAGC ACATTCACCG CCTCAAGCAT GAGCATATTG AGCGTCGGAG AAAGCTGTAC 
CTGGCAGCTC TGCCATTGGC TTTTGAAGCC CTCATACCTA ATCTAGATGA AGTGGACCAC 
CTGAGCTGTA TTAAAGCAAA AAAGCTGTTA GAGACCAAGC CAGAGTTCTT AAAGTGGTTT 
GTTGTACTTG AAGAAACACC ATGGGATGCC ACCAGCCACA TTGACAACAT GGAAAATGAG 
CGGATTCCCT TTGACTTGAT GGATACTGTC CCTGCTGAGC AGTTGTATGA GACCCACTTG 
GAGAAGCTGA GGAACGAGAG GAAGAGAGCT GAGATGCGAA GGGCTTTCAA AGAAAACCTG 
GAGACCTCTC CTTTCATAAC TCCTGGGAAA CCTTGGGAAG AAGCTCGTAG TTTTATTATG 
AATGAAGACT TCTACCAGTG GCTGGAAGAA TCTGTGTACA TGGACATCTA TGGCAAGCAC 
CAAAAGCAGA TTATAGACCG AGCAAAGGAA GAGTTCCAAG AGTTGCTTTT GGAGTACTCA 
GAATTGTTTT ATGAGCTGGA GCTGGACGCT AAGCCCAGCA AGGAGAAGAT GGGTGTCATC 
CAGGATGTTC TGGGCGAAGA GCAGAGATTT AAAGCATTGC AGAAGCTCCA AGCAGAGCGT 
GATGCCCTCA TTCTGAAGCA CATCCATTTT GTGTACCACC CGACGAAGGA GACATGCCCA 
AGCTGTCCAG CTTGTGTAGA TGCTAAGATT GAACACTTGA TCAGTTCTCG CTTTATCAGA 
CCATCTGATC GGAATCAGAA GAACTCTCTG TCTGACCTCA ATATTGATAG GATCAATTTG 
GTTATCTTAG GCAAAGATGG CCTTGCCCGA GAGTTGGCCA ATGAAATTCG AGCTCTTTGT 
ACAAATGATG ACAAGTATGT AATAGATGGT AAAATGTATG AGCTTTCTCT GAGGCCAATA 
GAGGGGAATG TCCGGCTTCC TGTGAACTCT TTCCAGACAC CAACCTTCCA ACCCCATGGC 
TGTCTCTGCC TTTACAATTC AAAGGAGTCA CTGTCCTATG TGGTGGAGAG TATAGAGAAG 
AGCAGAGAGT CTACACTGGG CAGGCGGGAT AATCATTTAG TCCACCTTCC CTTGACCTTA 
ATTTTAGTTA ACAAGAGAGG GGACACAAGT GGAGAGACTC TGCACAGCTT GATACAGCAA 
GGTCAGCAGA TTGCCAGCAA ACTTCAGTGT GTCTTTCTTG ATCCGGCTTC TGCTGGCATT 
GGTTATGGAC GCAACATTAA TGAGAAGCAG ATCAGTCAAG TTCTGAAAGG ACTCCTGGAC 
TCCAAGCGCA ACTTAAACCT GGTTAGCTCC ACTGCAAGTA TCAAAGATTT GGCTGATGTG 
GACCTTCGAA TTGTCATGTG TCTGATGTGT GGTGATCCCT TTAGTGCAGA TGACGTTCTC 
TCTCCTGTCC TGCAATCCCA AACTTGTAAA TCTTCCCACT GTGGGAGCAG CAACTCTGTT 
TTACTTGAAC TTCCAATTGG ACTACACAAG AAGCGCATTG AGCTGTCTGT TCTTTCATAC 
CATTCCTCAT TTAGCATCCG AAAGAGCCGG TTGGTTCATG GGTACATTGT TTTTTATTCA 
GCCAAACGTA AGGCCTCCTT GGCAATGTTA CGTGCCTTTC TTTGTGAAGT GCAGGATATT 
ATCCCCATCC AGCTTGTCGC ACTCACTGAT GGCGCTATAG ATGTCCTAGA CAATGACTTA 
AGTCGAGAGC AGCTAACAGA GGGAGAGGAA ATTGCACAAG AGATTGACGG GAGATTCACA 
AGCATCCCTT GTAGCCAGCC CCAGCATAAA CTTGAGCTCT TTCATCCCTT TTTTAAAGAT 
GTGGTGGAGA AAAAGAACAT AATTGAGGCC ACACATATGT ATGATAATGT AGCTGAGGCC 
TGCAGCACCA CTGAGGAAGT GTTCAACTCC CCCAGAGCTG GCTCACCCCT CTGCAACTCA 
AACTTACAGG ACTCAGAAGA AGATGTGGAG CCTCCATCAT ACCACCTGTT TCGGGAAGAT 
GCGACATTGC CCTCCCTGTC CAAAGATCAT TCCAAGTTCT CTATGGAACT GGAGGGAAAT 
GACGGGCTAT CTTTCATAAT GAGCAATTTT GAGAGTAAAC TGAACAACAA AGTACCTCCC 
CCAGTCAAAC CAAAGCCTCC TGTGCATTTT GATATCACAA AAGATCTTTC TTACTTAGAC 
CAAGGCCATC GGGAGGGACA GAGGAAATCT ATGTCTTCTA GCCCCTGGAT GCCTCAGGAT 
GGGTTTGATC CTTCTGACTA CGCAGAGCCC ATGGATGCTG TGGTCAAGCC AAGGAATGAG 
GAAGAAAACA TATACTCGGT GCCCCACGAC AGCACCCAGG GCAAGATCAT TACCATTCGG 
AACATCAACA AAGCCCAGTC CAATGGCAGT GGTAATGGTT CTGACAGTGA GATGGACACA 
AGTTCCCTAG AGCGAGGCCG CAAGGTATCT GCAGTGAGTA AGCCTGTGCT GTACAGGACA 
AGATGCACTC GCCTGGGGCG GTTTGCTAGT TACCGTACCA GCTTCAGTGT CGGGAGTGAT 
GATGAGCTGG GACCCATCCG AAAGAAAGAG GAGGACCAGG CATCCCAAGG TTATAAAGGG 
GACAATGCTG TCATTCCATA TGAAACAGAT GAGGACCCCA GGAGGAGGAA TATCCTTCGA 
AGTCTAAGAA GGAACACCAA GAAACCAAAG CCCAAACCCC GGCCATCCAT CACAAAGGCA 
ACCTGGGAGA GTAACTATTT TGGGGTGCCC TTAACAACAG TGGTGACTCC AGAGAAACCG 
ATACCCATTT TCATTGAAAG ATGCATTGAG TACATCGAAG CCACAGGACT AAGCACTGAA 
GGCATCTACC GGGTCAGTGG AAACAAGTCA GAAATGGAAA GTTTGCAAAG ACAGTTTGAT 
CAAGACCACA ATCTGGACCT GGCAGAGAAA GACTTCACTG TGAACACTGT GGCAGGGGCC 
ATGAAGAGTT TTTTCTCAGA GCTACCAGAC CCCCTGGTAC CATACAGCAT GCAGATTGAC 
TTGGTGGAAG CTCACAAAAT CAACGACAGG GAACAGAAGC TGCATGCTCT GAAGGAAGTG 
CTGAAGAAGT TCCCGAAGGA AAACCATGAA GTCTTCAAAT ATGTCATCTC CCACCTGAAC 
AAAGTCAGCC ACAACAACAA GGTGAATCTT ATGACCAGTG AGAACCTGTC CATCTGCTTC 
TGGCCCACGC TGATGCGGCC TGACTTCAGC AGCATGGACG CGCTCACAGC CACGCGCTCC 
TACCAGACCA TCATCGAGCT CTTCATCCAG CAGTGCCCCT TCTTCTTCTA CAACCGGCCC 
ATCAGCGAGC CTCCAGGGGC TGCGCCTGGC TCCCCTTCAG CCATGGCTCC CACTGTTCCC 
TTCCTCACCT CTACACCTGC TACCAGTCAG CCATCACCTC CCCAGTCACC TCCTCCAACC 
CCTCAGTCCC CAATGCAGCC ATTGCTGTCC TCTCAGCTCC AAGCCGAACA CACGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011248814.1
CDS	1022..5521
Translation	MMMARKQDVRIPTYNISVVGLSGTEKEKGQCGIGKSCLCNRFVRPSADEFHLDHTSVLSTSDFGGRVVNNDHFLYWGEVSRSLEDCVECKMHIVEQTEFIDDQTFQPHRSTALQPYIKRAAATKLASAEKLMYFCTDQLGLEQDFEQKQMPDGKLLVDGFLLGIDVSRGMNRNFDDQLKFVSNLYNQLAKTKKPIVVVLTKCDEGVERYIRDAHTFALSKKNLQVVETSARSNVNVDLAFSTLVQLIDKSRGKTKIIPYFEALKQQSQQIATAKDKYEWLVSRIVKNHNENWPSVSRKMQASPEYQDYVYLEGTQKAKKLFLQHIHRLKHEHIERRRKLYLAALPLAFEALIPNLDEVDHLSCIKAKKLLETKPEFLKWFVVLEETPWDATSHIDNMENERIPFDLMDTVPAEQLYETHLEKLRNERKRAEMRRAFKENLETSPFITPGKPWEEARSFIMNEDFYQWLEESVYMDIYGKHQKQIIDRAKEEFQELLLEYSELFYELELDAKPSKEKMGVIQDVLGEEQRFKALQKLQAERDALILKHIHFVYHPTKETCPSCPACVDAKIEHLISSRFIRPSDRNQKNSLSDLNIDRINLVILGKDGLARELANEIRALCTNDDKYVIDGKMYELSLRPIEGNVRLPVNSFQTPTFQPHGCLCLYNSKESLSYVVESIEKSRESTLGRRDNHLVHLPLTLILVNKRGDTSGETLHSLIQQGQQIASKLQCVFLDPASAGIGYGRNINEKQISQVLKGLLDSKRNLNLVSSTASIKDLADVDLRIVMCLMCGDPFSADDVLSPVLQSQTCKSSHCGSSNSVLLELPIGLHKKRIELSVLSYHSSFSIRKSRLVHGYIVFYSAKRKASLAMLRAFLCEVQDIIPIQLVALTDGAIDVLDNDLSREQLTEGEEIAQEIDGRFTSIPCSQPQHKLELFHPFFKDVVEKKNIIEATHMYDNVAEACSTTEEVFNSPRAGSPLCNSNLQDSEEDVEPPSYHLFREDATLPSLSKDHSKFSMELEGNDGLSFIMSNFESKLNNKVPPPVKPKPPVHFDITKDLSYLDQGHREGQRKSMSSSPWMPQDGFDPSDYAEPMDAVVKPRNEEENIYSVPHDSTQGKIITIRNINKAQSNGSGNGSDSEMDTSSLERGRKVSAVSKPVLYRTRCTRLGRFASYRTSFSVGSDDELGPIRKKEEDQASQGYKGDNAVIPYETDEDPRRRNILRSLRRNTKKPKPKPRPSITKATWESNYFGVPLTTVVTPEKPIPIFIERCIEYIEATGLSTEGIYRVSGNKSEMESLQRQFDQDHNLDLAEKDFTVNTVAGAMKSFFSELPDPLVPYSMQIDLVEAHKINDREQKLHALKEVLKKFPKENHEVFKYVISHLNKVSHNNKVNLMTSENLSICFWPTLMRPDFSSMDALTATRSYQTIIELFIQQCPFFFYNRPISEPPGAAPGSPSAMAPTVPFLTSTPATSQPSPPQSPPPTPQSPMQPLLSSQLQAEHTL

Target ORF information:

RefSeq Version	XM_011250512.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho GTPase activating protein 35 (Arhgap35), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011250512.2

ORF Insert Sequence:

ATGATGATGG CAAGAAAGCA AGATGTCCGC ATCCCCACCT ACAACATCAG TGTGGTGGGA 
TTGTCAGGCA CTGAGAAGGA GAAAGGCCAG TGCGGCATTG GGAAGTCTTG TCTGTGTAAC 
CGCTTTGTGC GCCCAAGTGC TGATGAGTTT CACCTGGACC ACACTTCTGT CCTTAGCACC 
AGTGACTTTG GTGGGCGGGT GGTCAATAAT GACCACTTTC TGTACTGGGG AGAAGTTAGC 
CGCTCCCTGG AGGATTGTGT GGAATGTAAG ATGCACATTG TGGAGCAGAC TGAGTTTATT 
GACGATCAGA CTTTTCAACC TCATCGAAGC ACGGCACTGC AGCCCTACAT CAAGAGAGCC 
GCAGCCACCA AGCTTGCTTC AGCTGAAAAA CTCATGTATT TTTGCACTGA CCAGCTGGGA 
CTGGAGCAAG ACTTTGAGCA GAAACAGATG CCAGATGGGA AGCTGCTGGT TGACGGTTTC 
CTTCTGGGCA TTGATGTTAG CAGGGGCATG AACAGGAACT TCGATGATCA GCTCAAATTT 
GTCTCCAATC TCTACAATCA GCTTGCAAAA ACAAAAAAGC CCATAGTGGT AGTCCTGACT 
AAGTGTGATG AGGGCGTTGA GCGGTACATT AGAGATGCAC ATACTTTTGC CTTAAGCAAA 
AAGAACCTCC AGGTTGTAGA GACCTCTGCA AGGTCCAATG TGAATGTGGA CTTGGCTTTC 
AGCACCTTAG TGCAACTCAT TGATAAGAGT CGAGGGAAGA CAAAAATCAT CCCTTACTTT 
GAAGCTCTCA AGCAGCAGAG TCAGCAGATA GCTACAGCAA AGGACAAGTA TGAGTGGTTA 
GTGAGCCGCA TTGTGAAAAA TCACAATGAG AACTGGCCGA GTGTCAGCCG AAAGATGCAG 
GCCTCCCCTG AGTACCAGGA CTATGTCTAC CTGGAAGGGA CACAGAAGGC CAAGAAGCTC 
TTCCTGCAGC ACATTCACCG CCTCAAGCAT GAGCATATTG AGCGTCGGAG AAAGCTGTAC 
CTGGCAGCTC TGCCATTGGC TTTTGAAGCC CTCATACCTA ATCTAGATGA AGTGGACCAC 
CTGAGCTGTA TTAAAGCAAA AAAGCTGTTA GAGACCAAGC CAGAGTTCTT AAAGTGGTTT 
GTTGTACTTG AAGAAACACC ATGGGATGCC ACCAGCCACA TTGACAACAT GGAAAATGAG 
CGGATTCCCT TTGACTTGAT GGATACTGTC CCTGCTGAGC AGTTGTATGA GACCCACTTG 
GAGAAGCTGA GGAACGAGAG GAAGAGAGCT GAGATGCGAA GGGCTTTCAA AGAAAACCTG 
GAGACCTCTC CTTTCATAAC TCCTGGGAAA CCTTGGGAAG AAGCTCGTAG TTTTATTATG 
AATGAAGACT TCTACCAGTG GCTGGAAGAA TCTGTGTACA TGGACATCTA TGGCAAGCAC 
CAAAAGCAGA TTATAGACCG AGCAAAGGAA GAGTTCCAAG AGTTGCTTTT GGAGTACTCA 
GAATTGTTTT ATGAGCTGGA GCTGGACGCT AAGCCCAGCA AGGAGAAGAT GGGTGTCATC 
CAGGATGTTC TGGGCGAAGA GCAGAGATTT AAAGCATTGC AGAAGCTCCA AGCAGAGCGT 
GATGCCCTCA TTCTGAAGCA CATCCATTTT GTGTACCACC CGACGAAGGA GACATGCCCA 
AGCTGTCCAG CTTGTGTAGA TGCTAAGATT GAACACTTGA TCAGTTCTCG CTTTATCAGA 
CCATCTGATC GGAATCAGAA GAACTCTCTG TCTGACCTCA ATATTGATAG GATCAATTTG 
GTTATCTTAG GCAAAGATGG CCTTGCCCGA GAGTTGGCCA ATGAAATTCG AGCTCTTTGT 
ACAAATGATG ACAAGTATGT AATAGATGGT AAAATGTATG AGCTTTCTCT GAGGCCAATA 
GAGGGGAATG TCCGGCTTCC TGTGAACTCT TTCCAGACAC CAACCTTCCA ACCCCATGGC 
TGTCTCTGCC TTTACAATTC AAAGGAGTCA CTGTCCTATG TGGTGGAGAG TATAGAGAAG 
AGCAGAGAGT CTACACTGGG CAGGCGGGAT AATCATTTAG TCCACCTTCC CTTGACCTTA 
ATTTTAGTTA ACAAGAGAGG GGACACAAGT GGAGAGACTC TGCACAGCTT GATACAGCAA 
GGTCAGCAGA TTGCCAGCAA ACTTCAGTGT GTCTTTCTTG ATCCGGCTTC TGCTGGCATT 
GGTTATGGAC GCAACATTAA TGAGAAGCAG ATCAGTCAAG TTCTGAAAGG ACTCCTGGAC 
TCCAAGCGCA ACTTAAACCT GGTTAGCTCC ACTGCAAGTA TCAAAGATTT GGCTGATGTG 
GACCTTCGAA TTGTCATGTG TCTGATGTGT GGTGATCCCT TTAGTGCAGA TGACGTTCTC 
TCTCCTGTCC TGCAATCCCA AACTTGTAAA TCTTCCCACT GTGGGAGCAG CAACTCTGTT 
TTACTTGAAC TTCCAATTGG ACTACACAAG AAGCGCATTG AGCTGTCTGT TCTTTCATAC 
CATTCCTCAT TTAGCATCCG AAAGAGCCGG TTGGTTCATG GGTACATTGT TTTTTATTCA 
GCCAAACGTA AGGCCTCCTT GGCAATGTTA CGTGCCTTTC TTTGTGAAGT GCAGGATATT 
ATCCCCATCC AGCTTGTCGC ACTCACTGAT GGCGCTATAG ATGTCCTAGA CAATGACTTA 
AGTCGAGAGC AGCTAACAGA GGGAGAGGAA ATTGCACAAG AGATTGACGG GAGATTCACA 
AGCATCCCTT GTAGCCAGCC CCAGCATAAA CTTGAGCTCT TTCATCCCTT TTTTAAAGAT 
GTGGTGGAGA AAAAGAACAT AATTGAGGCC ACACATATGT ATGATAATGT AGCTGAGGCC 
TGCAGCACCA CTGAGGAAGT GTTCAACTCC CCCAGAGCTG GCTCACCCCT CTGCAACTCA 
AACTTACAGG ACTCAGAAGA AGATGTGGAG CCTCCATCAT ACCACCTGTT TCGGGAAGAT 
GCGACATTGC CCTCCCTGTC CAAAGATCAT TCCAAGTTCT CTATGGAACT GGAGGGAAAT 
GACGGGCTAT CTTTCATAAT GAGCAATTTT GAGAGTAAAC TGAACAACAA AGTACCTCCC 
CCAGTCAAAC CAAAGCCTCC TGTGCATTTT GATATCACAA AAGATCTTTC TTACTTAGAC 
CAAGGCCATC GGGAGGGACA GAGGAAATCT ATGTCTTCTA GCCCCTGGAT GCCTCAGGAT 
GGGTTTGATC CTTCTGACTA CGCAGAGCCC ATGGATGCTG TGGTCAAGCC AAGGAATGAG 
GAAGAAAACA TATACTCGGT GCCCCACGAC AGCACCCAGG GCAAGATCAT TACCATTCGG 
AACATCAACA AAGCCCAGTC CAATGGCAGT GGTAATGGTT CTGACAGTGA GATGGACACA 
AGTTCCCTAG AGCGAGGCCG CAAGGTATCT GCAGTGAGTA AGCCTGTGCT GTACAGGACA 
AGATGCACTC GCCTGGGGCG GTTTGCTAGT TACCGTACCA GCTTCAGTGT CGGGAGTGAT 
GATGAGCTGG GACCCATCCG AAAGAAAGAG GAGGACCAGG CATCCCAAGG TTATAAAGGG 
GACAATGCTG TCATTCCATA TGAAACAGAT GAGGACCCCA GGAGGAGGAA TATCCTTCGA 
AGTCTAAGAA GGAACACCAA GAAACCAAAG CCCAAACCCC GGCCATCCAT CACAAAGGCA 
ACCTGGGAGA GTAACTATTT TGGGGTGCCC TTAACAACAG TGGTGACTCC AGAGAAACCG 
ATACCCATTT TCATTGAAAG ATGCATTGAG TACATCGAAG CCACAGGACT AAGCACTGAA 
GGCATCTACC GGGTCAGTGG AAACAAGTCA GAAATGGAAA GTTTGCAAAG ACAGTTTGAT 
CAAGACCACA ATCTGGACCT GGCAGAGAAA GACTTCACTG TGAACACTGT GGCAGGGGCC 
ATGAAGAGTT TTTTCTCAGA GCTACCAGAC CCCCTGGTAC CATACAGCAT GCAGATTGAC 
TTGGTGGAAG CTCACAAAAT CAACGACAGG GAACAGAAGC TGCATGCTCT GAAGGAAGTG 
CTGAAGAAGT TCCCGAAGGA AAACCATGAA GTCTTCAAAT ATGTCATCTC CCACCTGAAC 
AAAGTCAGCC ACAACAACAA GGTGAATCTT ATGACCAGTG AGAACCTGTC CATCTGCTTC 
TGGCCCACGC TGATGCGGCC TGACTTCAGC AGCATGGACG CGCTCACAGC CACGCGCTCC 
TACCAGACCA TCATCGAGCT CTTCATCCAG CAGTGCCCCT TCTTCTTCTA CAACCGGCCC 
ATCAGCGAGC CTCCAGGGGC TGCGCCTGGC TCCCCTTCAG CCATGGCTCC CACTGTTCCC 
TTCCTCACCT CTACACCTGC TACCAGTCAG CCATCACCTC CCCAGTCACC TCCTCCAACC 
CCTCAGTCCC CAATGCAGCC ATTGCTGTCC TCTCAGCTCC AAGCCGAACA CACGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A Point Mutation in p190A RhoGAP Affects Ciliogenesis and Leads to Glomerulocystic Kidney Defects.
PLoS genetics12(2)e1005785(2016 Feb)
Stewart K,Gaitan Y,Shafer ME,Aoudjit L,Hu D,Sharma R,Tremblay M,Ishii H,Marcotte M,Stanga D,Tang YC,Boualia SK,Nguyen AH,Takano T,Lamarche-Vane N,Vidal S,Bouchard M

An AUTS2-Polycomb complex activates gene expression in the CNS.
Nature516(7531)349-54(2014 Dec)
Gao Z,Lee P,Stafford JM,von Schimmelmann M,Schaefer A,Reinberg D

p190RhoGAP has cellular RacGAP activity regulated by a polybasic region.
Cellular signalling25(6)1388-94(2013 Jun)
L?vay M,Bartos B,Ligeti E

Integrin ?3 is required for late postnatal stability of dendrite arbors, dendritic spines and synapses, and mouse behavior.
The Journal of neuroscience : the official journal of the Society for Neuroscience33(16)6742-52(2013 Apr)
Kerrisk ME,Greer CA,Koleske AJ

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Arhgap35 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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