Arhgap25 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Arhgap25
Entrez Gene ID	232201
Full Name	Rho GTPase activating protein 25
Synonyms	A130039I20Rik
General protein information	Preferred Names Rho GTPase activating protein 25 Names rho GTPase-activating protein 25 rho-type GTPase-activating protein 25
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6\|6 D1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006505980.3	XP_006506043.1	rho GTPase-activating protein 25 isoform X2
NM_001286610.1	NP_001273539.1	rho GTPase-activating protein 25 isoform c
XM_017321530.1	XP_017177019.1	rho GTPase-activating protein 25 isoform X1
XM_017321531.1	XP_017177020.1	rho GTPase-activating protein 25 isoform X1
NM_175476.4	NP_780685.2	rho GTPase-activating protein 25 isoform b
NM_001037727.2	NP_001032816.1	rho GTPase-activating protein 25 isoform a

Pathways from BioSystems

Homologies

Bos taurus (cattle)	ARHGAP25	NP_001039857.1
Rattus norvegicus (Norway rat)	Arhgap25	NP_001102717.1
Mus musculus (house mouse)	Arhgap25	NP_001032816.1
Gallus gallus (chicken)	ARHGAP25	NP_001026054.1
Pan troglodytes (chimpanzee)	ARHGAP25	XP_001135470.1
Canis lupus familiaris (dog)	ARHGAP25	XP_005626306.1
Danio rerio (zebrafish)	arhgap25	NP_001119876.1
Homo sapiens (human)	ARHGAP25	NP_001007232.2
	ARHGAP25	XP_001095743.1
Xenopus tropicalis (tropical clawed frog)	arhgap25	NP_001006710.1

Gene Ontology
Bibliography

Related articles in PubMed

Rac GTPase Activating Protein ARHGAP25 Regulates Leukocyte Transendothelial Migration in Mice.
Cs?p?nyi-K?mi R, Wisniewski ?, Bartos B, L?vai P, N?meth T, Bal?zs B, Kurz AR, Bierschenk S, Sperandio M, Ligeti E
Journal of immunology (Baltimore, Md. : 1950)197(7)2807-15(2016 Oct)

Phosphoproteomic profiling of mouse primary HSPCs reveals new regulators of HSPC mobilization.
Wang LD, Ficarro SB, Hutchinson JN, Csepanyi-Komi R, Nguyen PT, Wisniewski E, Sullivan J, Hofmann O, Ligeti E, Marto JA, Wagers AJ
Blood128(11)1465-74(2016 09)

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Thompson CL, Ng L, Menon V, Martinez S, Lee CK, Glattfelder K, Sunkin SM, Henry A, Lau C, Dang C, Garcia-Lopez R, Martinez-Ferre A, Pombero A, Rubenstein JLR, Wakeman WB, Hohmann J, Dee N, Sodt AJ, Young R, Smith K, Nguyen TN, Kidney J, Kuan L, Jeromin A, Kaykas A, Miller J, Page D, Orta G, Bernard A, Riley Z, Smith S, Wohnoutka P, Hawrylycz MJ, Puelles L, Jones AR
Neuron83(2)309-323(2014 Jul)

Identification and characterization of ARHGAP24 and ARHGAP25 genes in silico.
Katoh M, Katoh M
International journal of molecular medicine14(2)333-8(2004 Aug)

Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
Okazaki N, F-Kikuno R, Ohara R, Inamoto S, Koseki H, Hiraoka S, Saga Y, Seino S, Nishimura M, Kaisho T, Hoshino K, Kitamura H, Nagase T, Ohara O, Koga H
DNA research : an international journal for rapid publication of reports on genes and genomes11(3)205-18(2004 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Using Arhgap25-deficient mice, it identified ARHGAP25 as a relevant negative regulator of leukocyte transendothelial migration.
Title: Rac GTPase Activating Protein ARHGAP25 Regulates Leukocyte Transendothelial Migration in Mice.

The following Arhgap25 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Arhgap25 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu05452	NM_175476.4 Latest version!	Mus musculus Rho GTPase activating protein 25 (Arhgap25), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$370.30 $529.00
OMu12831	NM_001037727.2 Latest version!	Mus musculus Rho GTPase activating protein 25 (Arhgap25), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$384.30 $549.00
OMu21618	NM_001286610.1 Latest version!	Mus musculus Rho GTPase activating protein 25 (Arhgap25), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu37171	XM_006505980.3 Latest version!	Mus musculus Rho GTPase activating protein 25 (Arhgap25), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu81697	XM_017321530.1 Latest version!	Mus musculus Rho GTPase activating protein 25 (Arhgap25), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu81698	XM_017321531.1 Latest version!	Mus musculus Rho GTPase activating protein 25 (Arhgap25), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Arhgap25 ( NM_175476.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_780685.2 Mus musculus Rho GTPase activating protein 25 (Arhgap25), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu05452
Clone ID Related Accession (Same CDS sequence)	NM_175476.4
Accession Version	NM_175476.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	rho GTPase-activating protein 25 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ145934.1, AK137444.1 and AK137526.1. On Nov 8, 2013 this sequence version replaced NM_175476.3. Transcript Variant: This variant (2) contains an alternate 5' terminal exon and initiates translation at a downstream start codon, compared to variant 1. It encodes isoform b, which has a shorter N-terminus, compared to isoform a. ##Evidence-Data-START## Transcript exon combination :: AK048450.1, AK137444.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCGGGG AGCAGATGGC GGCCTTTCAC CCACCCACCA CTCCCAACCC CCTGGAAAGG 
CCAATTAAGG TGGGCTGGCT GAAGAAGCAG AGGTCCATCG TGAAAAACTG GCAGCAGCGG 
TACTTTGTGT TGAGAGCTCA GCAACTCTAC TACTACAAGG ACGAAGAGGA CTCAAAGCCA 
CAGGGCTGCA TGTATCTGCC AGGCAGTACA GTCAAAGAAA TTGCCACAAA CCCCGAAGAA 
GCTGGGAAAT TTGTCTTTGA AGTTATTCCA GCCTCAAGTG ACCAGAACCG CATTGGACAA 
GACTCCTACG TCCTCATGGC CAGCTCCCAG GTAGAGATGG AGGAGTGGGT TAAGTTCCTC 
AGGAGAGTTG CTGGCACGCC CTCTGGAGCG GTGTTTGGCC AGCGTCTGGA TGAGACTGTG 
GCCTATGAGC AGAAGTTTGG CCCTCACCTG GTGCCCATCT TGGTGGAGAA GTGCGCTGAG 
TTCATCCTGG AACATGGTGT GAGTGAAGAG GGCATCTTCC GCCTGCCCGG GCAGGACAAC 
CTTGTGAAGC AGCTGAGAGA TGCTTTCGAT GCGGGGGAGC GGCCCTCCTT TGACAGGGAC 
ACAGATGTGC ACACGGTGGC ATCTCTACTA AAGCTCTACC TCCGAGACCT GCCAGAGCCT 
GTGGTTCCTT GGAGTCAGTA TGAAGGGTTC CTGCTCTGTG GGCAGCTCAT GAACGCAGAT 
GAGGCAAAGG CTCAGCAGGA GTTGGTGAAG CAGCTTTCTA CCCTTCCCCG AGACAACTAC 
AACCTCCTGA GCTACATCTG CAGATTTCTG CATGAAATCC AGCTGAACTG TGCCGTCAAC 
AAGATGAGCG TGGACAACCT GGCCACTGTG ATCGGGGTGA ACCTCATCAG GTCGAAGGTT 
GAAGACCCAG CTGTGATTAT GAGAGGGACT CCTCAGATCC AAAGAGTGAT GACCATGATG 
ATCAGAGACC ACGAAGTCCT CTTCCCCAAG TCTAAGGATG CACCGATCTC ACCCCCTGCC 
CAGAAAAACG ATGCCAAGAA GGCTCCAGTG CCGCGAAGCT CTGTGGGGTG GGATGCCACG 
GAAGACCCAC CCCTTTCTAG GACAGACAGC TTCAGTAACA CAGCAAGTAG TCCTGATGCC 
ACCAGTCCCA CTGGACCACT GCCCAGTGAC CAGCATCAGG AAGACAGCGG AAAAGCCCCC 
AGGGAAAACC CAGGAGACTG GAAGATGCAA TCCCGTAAAA GGACTCAAAC GCTTCCCAAC 
CGGAAGTGTT TCCTGACGTC CGCATTCCAA GGCACCACCA GCAGTAAACT GGAAATCTTT 
AAAAATGAGT TCTGGTCTCC ATCTTCAGAG GCTAAGGCAG GAGAAGGGCA CAGGCGAACT 
ATGTCCCAAG ACTTGCGCCA CCTTTCCAAT GACCAGCGGA CTTCTACCTA CGATAATGTC 
CCCACCTCAC CACAGTCCCA AGGGAACCCA GCAGGTGCAC TCTCTCCCCC TGCCAGTGAC 
TCCAAGAGAG ATGCTCTTGT TAGCACAGAC TCTGAAATGG AGGCTGGAAG CAAGAACTCT 
GGCGAGGATG ACCTTGATTC TCTGCAGAGG ACAGTCCAGA GCCTACAGAA GGAAATAGAA 
ACCCAGAAGC AGGTCTATGA GGAACAGATT AAAAACCTGG AGAAGGAAAA TTACGATGTC 
TGGGCTAAGG TGGTGAGGCT CAATGAAGAA CTCGAGAGGG AGAGGAAGAA ATTCGCGGCC 
CTGGAAATCA GCCTTCGAAA TGTGGAGCGC TCCCGGGAGG ACGTTGAGAA GAGGAACAGA 
GTCTTGGAAG AAGAAGTCAA GGAGTTTGTG AAGTCGATGG AGAAGCCCAA GACAAAGACG 
GATCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_780685.2
CDS	123..1991
Translation	MTGEQMAAFHPPTTPNPLERPIKVGWLKKQRSIVKNWQQRYFVLRAQQLYYYKDEEDSKPQGCMYLPGSTVKEIATNPEEAGKFVFEVIPASSDQNRIGQDSYVLMASSQVEMEEWVKFLRRVAGTPSGAVFGQRLDETVAYEQKFGPHLVPILVEKCAEFILEHGVSEEGIFRLPGQDNLVKQLRDAFDAGERPSFDRDTDVHTVASLLKLYLRDLPEPVVPWSQYEGFLLCGQLMNADEAKAQQELVKQLSTLPRDNYNLLSYICRFLHEIQLNCAVNKMSVDNLATVIGVNLIRSKVEDPAVIMRGTPQIQRVMTMMIRDHEVLFPKSKDAPISPPAQKNDAKKAPVPRSSVGWDATEDPPLSRTDSFSNTASSPDATSPTGPLPSDQHQEDSGKAPRENPGDWKMQSRKRTQTLPNRKCFLTSAFQGTTSSKLEIFKNEFWSPSSEAKAGEGHRRTMSQDLRHLSNDQRTSTYDNVPTSPQSQGNPAGALSPPASDSKRDALVSTDSEMEAGSKNSGEDDLDSLQRTVQSLQKEIETQKQVYEEQIKNLEKENYDVWAKVVRLNEELERERKKFAALEISLRNVERSREDVEKRNRVLEEEVKEFVKSMEKPKTKTDP

Target ORF information:

RefSeq Version	NM_175476.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho GTPase activating protein 25 (Arhgap25), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175476.4

ORF Insert Sequence:

ATGACCGGGG AGCAGATGGC GGCCTTTCAC CCACCCACCA CTCCCAACCC CCTGGAAAGG 
CCAATTAAGG TGGGCTGGCT GAAGAAGCAG AGGTCCATCG TGAAAAACTG GCAGCAGCGG 
TACTTTGTGT TGAGAGCTCA GCAACTCTAC TACTACAAGG ACGAAGAGGA CTCAAAGCCA 
CAGGGCTGCA TGTATCTGCC AGGCAGTACA GTCAAAGAAA TTGCCACAAA CCCCGAAGAA 
GCTGGGAAAT TTGTCTTTGA AGTTATTCCA GCCTCAAGTG ACCAGAACCG CATTGGACAA 
GACTCCTACG TCCTCATGGC CAGCTCCCAG GTAGAGATGG AGGAGTGGGT TAAGTTCCTC 
AGGAGAGTTG CTGGCACGCC CTCTGGAGCG GTGTTTGGCC AGCGTCTGGA TGAGACTGTG 
GCCTATGAGC AGAAGTTTGG CCCTCACCTG GTGCCCATCT TGGTGGAGAA GTGCGCTGAG 
TTCATCCTGG AACATGGTGT GAGTGAAGAG GGCATCTTCC GCCTGCCCGG GCAGGACAAC 
CTTGTGAAGC AGCTGAGAGA TGCTTTCGAT GCGGGGGAGC GGCCCTCCTT TGACAGGGAC 
ACAGATGTGC ACACGGTGGC ATCTCTACTA AAGCTCTACC TCCGAGACCT GCCAGAGCCT 
GTGGTTCCTT GGAGTCAGTA TGAAGGGTTC CTGCTCTGTG GGCAGCTCAT GAACGCAGAT 
GAGGCAAAGG CTCAGCAGGA GTTGGTGAAG CAGCTTTCTA CCCTTCCCCG AGACAACTAC 
AACCTCCTGA GCTACATCTG CAGATTTCTG CATGAAATCC AGCTGAACTG TGCCGTCAAC 
AAGATGAGCG TGGACAACCT GGCCACTGTG ATCGGGGTGA ACCTCATCAG GTCGAAGGTT 
GAAGACCCAG CTGTGATTAT GAGAGGGACT CCTCAGATCC AAAGAGTGAT GACCATGATG 
ATCAGAGACC ACGAAGTCCT CTTCCCCAAG TCTAAGGATG CACCGATCTC ACCCCCTGCC 
CAGAAAAACG ATGCCAAGAA GGCTCCAGTG CCGCGAAGCT CTGTGGGGTG GGATGCCACG 
GAAGACCCAC CCCTTTCTAG GACAGACAGC TTCAGTAACA CAGCAAGTAG TCCTGATGCC 
ACCAGTCCCA CTGGACCACT GCCCAGTGAC CAGCATCAGG AAGACAGCGG AAAAGCCCCC 
AGGGAAAACC CAGGAGACTG GAAGATGCAA TCCCGTAAAA GGACTCAAAC GCTTCCCAAC 
CGGAAGTGTT TCCTGACGTC CGCATTCCAA GGCACCACCA GCAGTAAACT GGAAATCTTT 
AAAAATGAGT TCTGGTCTCC ATCTTCAGAG GCTAAGGCAG GAGAAGGGCA CAGGCGAACT 
ATGTCCCAAG ACTTGCGCCA CCTTTCCAAT GACCAGCGGA CTTCTACCTA CGATAATGTC 
CCCACCTCAC CACAGTCCCA AGGGAACCCA GCAGGTGCAC TCTCTCCCCC TGCCAGTGAC 
TCCAAGAGAG ATGCTCTTGT TAGCACAGAC TCTGAAATGG AGGCTGGAAG CAAGAACTCT 
GGCGAGGATG ACCTTGATTC TCTGCAGAGG ACAGTCCAGA GCCTACAGAA GGAAATAGAA 
ACCCAGAAGC AGGTCTATGA GGAACAGATT AAAAACCTGG AGAAGGAAAA TTACGATGTC 
TGGGCTAAGG TGGTGAGGCT CAATGAAGAA CTCGAGAGGG AGAGGAAGAA ATTCGCGGCC 
CTGGAAATCA GCCTTCGAAA TGTGGAGCGC TCCCGGGAGG ACGTTGAGAA GAGGAACAGA 
GTCTTGGAAG AAGAAGTCAA GGAGTTTGTG AAGTCGATGG AGAAGCCCAA GACAAAGACG 
GATCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgap25 ( NM_001037727.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001032816.1 Mus musculus Rho GTPase activating protein 25 (Arhgap25), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu12831
Clone ID Related Accession (Same CDS sequence)	NM_001037727.2
Accession Version	NM_001037727.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	rho GTPase-activating protein 25 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK159941.1, AK037710.1 and AK137526.1. On Nov 8, 2013 this sequence version replaced NM_001037727.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). ##Evidence-Data-START## Transcript exon combination :: SRR1660819.152499.1, AK037710.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTCTCA AGCTGCCAAG GAACTGGGAT TTCAATTTGA AAGCGGAGGC TTCAAAAATA 
GCTCGGTCGA GGAGTGTGAT GACCGGGGAG CAGATGGCGG CCTTTCACCC ACCCACCACT 
CCCAACCCCC TGGAAAGGCC AATTAAGGTG GGCTGGCTGA AGAAGCAGAG GTCCATCGTG 
AAAAACTGGC AGCAGCGGTA CTTTGTGTTG AGAGCTCAGC AACTCTACTA CTACAAGGAC 
GAAGAGGACT CAAAGCCACA GGGCTGCATG TATCTGCCAG GCAGTACAGT CAAAGAAATT 
GCCACAAACC CCGAAGAAGC TGGGAAATTT GTCTTTGAAG TTATTCCAGC CTCAAGTGAC 
CAGAACCGCA TTGGACAAGA CTCCTACGTC CTCATGGCCA GCTCCCAGGT AGAGATGGAG 
GAGTGGGTTA AGTTCCTCAG GAGAGTTGCT GGCACGCCCT CTGGAGCGGT GTTTGGCCAG 
CGTCTGGATG AGACTGTGGC CTATGAGCAG AAGTTTGGCC CTCACCTGGT GCCCATCTTG 
GTGGAGAAGT GCGCTGAGTT CATCCTGGAA CATGGTGTGA GTGAAGAGGG CATCTTCCGC 
CTGCCCGGGC AGGACAACCT TGTGAAGCAG CTGAGAGATG CTTTCGATGC GGGGGAGCGG 
CCCTCCTTTG ACAGGGACAC AGATGTGCAC ACGGTGGCAT CTCTACTAAA GCTCTACCTC 
CGAGACCTGC CAGAGCCTGT GGTTCCTTGG AGTCAGTATG AAGGGTTCCT GCTCTGTGGG 
CAGCTCATGA ACGCAGATGA GGCAAAGGCT CAGCAGGAGT TGGTGAAGCA GCTTTCTACC 
CTTCCCCGAG ACAACTACAA CCTCCTGAGC TACATCTGCA GATTTCTGCA TGAAATCCAG 
CTGAACTGTG CCGTCAACAA GATGAGCGTG GACAACCTGG CCACTGTGAT CGGGGTGAAC 
CTCATCAGGT CGAAGGTTGA AGACCCAGCT GTGATTATGA GAGGGACTCC TCAGATCCAA 
AGAGTGATGA CCATGATGAT CAGAGACCAC GAAGTCCTCT TCCCCAAGTC TAAGGATGCA 
CCGATCTCAC CCCCTGCCCA GAAAAACGAT GCCAAGAAGG CTCCAGTGCC GCGAAGCTCT 
GTGGGGTGGG ATGCCACGGA AGACCCACCC CTTTCTAGGA CAGACAGCTT CAGTAACACA 
GCAAGTAGTC CTGATGCCAC CAGTCCCACT GGACCACTGC CCAGTGACCA GCATCAGGAA 
GACAGCGGAA AAGCCCCCAG GGAAAACCCA GGAGACTGGA AGATGCAATC CCGTAAAAGG 
ACTCAAACGC TTCCCAACCG GAAGTGTTTC CTGACGTCCG CATTCCAAGG CACCACCAGC 
AGTAAACTGG AAATCTTTAA AAATGAGTTC TGGTCTCCAT CTTCAGAGGC TAAGGCAGGA 
GAAGGGCACA GGCGAACTAT GTCCCAAGAC TTGCGCCACC TTTCCAATGA CCAGCGGACT 
TCTACCTACG ATAATGTCCC CACCTCACCA CAGTCCCAAG GGAACCCAGC AGGTGCACTC 
TCTCCCCCTG CCAGTGACTC CAAGAGAGAT GCTCTTGTTA GCACAGACTC TGAAATGGAG 
GCTGGAAGCA AGAACTCTGG CGAGGATGAC CTTGATTCTC TGCAGAGGAC AGTCCAGAGC 
CTACAGAAGG AAATAGAAAC CCAGAAGCAG GTCTATGAGG AACAGATTAA AAACCTGGAG 
AAGGAAAATT ACGATGTCTG GGCTAAGGTG GTGAGGCTCA ATGAAGAACT CGAGAGGGAG 
AGGAAGAAAT TCGCGGCCCT GGAAATCAGC CTTCGAAATG TGGAGCGCTC CCGGGAGGAC 
GTTGAGAAGA GGAACAGAGT CTTGGAAGAA GAAGTCAAGG AGTTTGTGAA GTCGATGGAG 
AAGCCCAAGA CAAAGACGGA TCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001032816.1
CDS	374..2320
Translation	MSLKLPRNWDFNLKAEASKIARSRSVMTGEQMAAFHPPTTPNPLERPIKVGWLKKQRSIVKNWQQRYFVLRAQQLYYYKDEEDSKPQGCMYLPGSTVKEIATNPEEAGKFVFEVIPASSDQNRIGQDSYVLMASSQVEMEEWVKFLRRVAGTPSGAVFGQRLDETVAYEQKFGPHLVPILVEKCAEFILEHGVSEEGIFRLPGQDNLVKQLRDAFDAGERPSFDRDTDVHTVASLLKLYLRDLPEPVVPWSQYEGFLLCGQLMNADEAKAQQELVKQLSTLPRDNYNLLSYICRFLHEIQLNCAVNKMSVDNLATVIGVNLIRSKVEDPAVIMRGTPQIQRVMTMMIRDHEVLFPKSKDAPISPPAQKNDAKKAPVPRSSVGWDATEDPPLSRTDSFSNTASSPDATSPTGPLPSDQHQEDSGKAPRENPGDWKMQSRKRTQTLPNRKCFLTSAFQGTTSSKLEIFKNEFWSPSSEAKAGEGHRRTMSQDLRHLSNDQRTSTYDNVPTSPQSQGNPAGALSPPASDSKRDALVSTDSEMEAGSKNSGEDDLDSLQRTVQSLQKEIETQKQVYEEQIKNLEKENYDVWAKVVRLNEELERERKKFAALEISLRNVERSREDVEKRNRVLEEEVKEFVKSMEKPKTKTDP

Target ORF information:

RefSeq Version	NM_001037727.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho GTPase activating protein 25 (Arhgap25), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001037727.2

ORF Insert Sequence:

ATGTCTCTCA AGCTGCCAAG GAACTGGGAT TTCAATTTGA AAGCGGAGGC TTCAAAAATA 
GCTCGGTCGA GGAGTGTGAT GACCGGGGAG CAGATGGCGG CCTTTCACCC ACCCACCACT 
CCCAACCCCC TGGAAAGGCC AATTAAGGTG GGCTGGCTGA AGAAGCAGAG GTCCATCGTG 
AAAAACTGGC AGCAGCGGTA CTTTGTGTTG AGAGCTCAGC AACTCTACTA CTACAAGGAC 
GAAGAGGACT CAAAGCCACA GGGCTGCATG TATCTGCCAG GCAGTACAGT CAAAGAAATT 
GCCACAAACC CCGAAGAAGC TGGGAAATTT GTCTTTGAAG TTATTCCAGC CTCAAGTGAC 
CAGAACCGCA TTGGACAAGA CTCCTACGTC CTCATGGCCA GCTCCCAGGT AGAGATGGAG 
GAGTGGGTTA AGTTCCTCAG GAGAGTTGCT GGCACGCCCT CTGGAGCGGT GTTTGGCCAG 
CGTCTGGATG AGACTGTGGC CTATGAGCAG AAGTTTGGCC CTCACCTGGT GCCCATCTTG 
GTGGAGAAGT GCGCTGAGTT CATCCTGGAA CATGGTGTGA GTGAAGAGGG CATCTTCCGC 
CTGCCCGGGC AGGACAACCT TGTGAAGCAG CTGAGAGATG CTTTCGATGC GGGGGAGCGG 
CCCTCCTTTG ACAGGGACAC AGATGTGCAC ACGGTGGCAT CTCTACTAAA GCTCTACCTC 
CGAGACCTGC CAGAGCCTGT GGTTCCTTGG AGTCAGTATG AAGGGTTCCT GCTCTGTGGG 
CAGCTCATGA ACGCAGATGA GGCAAAGGCT CAGCAGGAGT TGGTGAAGCA GCTTTCTACC 
CTTCCCCGAG ACAACTACAA CCTCCTGAGC TACATCTGCA GATTTCTGCA TGAAATCCAG 
CTGAACTGTG CCGTCAACAA GATGAGCGTG GACAACCTGG CCACTGTGAT CGGGGTGAAC 
CTCATCAGGT CGAAGGTTGA AGACCCAGCT GTGATTATGA GAGGGACTCC TCAGATCCAA 
AGAGTGATGA CCATGATGAT CAGAGACCAC GAAGTCCTCT TCCCCAAGTC TAAGGATGCA 
CCGATCTCAC CCCCTGCCCA GAAAAACGAT GCCAAGAAGG CTCCAGTGCC GCGAAGCTCT 
GTGGGGTGGG ATGCCACGGA AGACCCACCC CTTTCTAGGA CAGACAGCTT CAGTAACACA 
GCAAGTAGTC CTGATGCCAC CAGTCCCACT GGACCACTGC CCAGTGACCA GCATCAGGAA 
GACAGCGGAA AAGCCCCCAG GGAAAACCCA GGAGACTGGA AGATGCAATC CCGTAAAAGG 
ACTCAAACGC TTCCCAACCG GAAGTGTTTC CTGACGTCCG CATTCCAAGG CACCACCAGC 
AGTAAACTGG AAATCTTTAA AAATGAGTTC TGGTCTCCAT CTTCAGAGGC TAAGGCAGGA 
GAAGGGCACA GGCGAACTAT GTCCCAAGAC TTGCGCCACC TTTCCAATGA CCAGCGGACT 
TCTACCTACG ATAATGTCCC CACCTCACCA CAGTCCCAAG GGAACCCAGC AGGTGCACTC 
TCTCCCCCTG CCAGTGACTC CAAGAGAGAT GCTCTTGTTA GCACAGACTC TGAAATGGAG 
GCTGGAAGCA AGAACTCTGG CGAGGATGAC CTTGATTCTC TGCAGAGGAC AGTCCAGAGC 
CTACAGAAGG AAATAGAAAC CCAGAAGCAG GTCTATGAGG AACAGATTAA AAACCTGGAG 
AAGGAAAATT ACGATGTCTG GGCTAAGGTG GTGAGGCTCA ATGAAGAACT CGAGAGGGAG 
AGGAAGAAAT TCGCGGCCCT GGAAATCAGC CTTCGAAATG TGGAGCGCTC CCGGGAGGAC 
GTTGAGAAGA GGAACAGAGT CTTGGAAGAA GAAGTCAAGG AGTTTGTGAA GTCGATGGAG 
AAGCCCAAGA CAAAGACGGA TCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgap25 ( NM_001286610.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001273539.1 Mus musculus Rho GTPase activating protein 25 (Arhgap25), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu21618
Clone ID Related Accession (Same CDS sequence)	NM_001286610.1
Accession Version	NM_001286610.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	rho GTPase-activating protein 25 isoform c
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK159941.1 and AK137526.1. Transcript Variant: This variant (3) lacks an alternate exon in the 5' coding region and initiates translation at a downstream start codon, compared to variant 1. It encodes isoform c, which is shorter at the N-terminus, compared to isoform a. ##Evidence-Data-START## Transcript exon combination :: AK159941.1, AK159598.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTATCTGC CAGGCAGTAC AGTCAAAGAA ATTGCCACAA ACCCCGAAGA AGCTGGGAAA 
TTTGTCTTTG AAGTTATTCC AGCCTCAAGT GACCAGAACC GCATTGGACA AGACTCCTAC 
GTCCTCATGG CCAGCTCCCA GGTAGAGATG GAGGAGTGGG TTAAGTTCCT CAGGAGAGTT 
GCTGGCACGC CCTCTGGAGC GGTGTTTGGC CAGCGTCTGG ATGAGACTGT GGCCTATGAG 
CAGAAGTTTG GCCCTCACCT GGTGCCCATC TTGGTGGAGA AGTGCGCTGA GTTCATCCTG 
GAACATGGTG TGAGTGAAGA GGGCATCTTC CGCCTGCCCG GGCAGGACAA CCTTGTGAAG 
CAGCTGAGAG ATGCTTTCGA TGCGGGGGAG CGGCCCTCCT TTGACAGGGA CACAGATGTG 
CACACGGTGG CATCTCTACT AAAGCTCTAC CTCCGAGACC TGCCAGAGCC TGTGGTTCCT 
TGGAGTCAGT ATGAAGGGTT CCTGCTCTGT GGGCAGCTCA TGAACGCAGA TGAGGCAAAG 
GCTCAGCAGG AGTTGGTGAA GCAGCTTTCT ACCCTTCCCC GAGACAACTA CAACCTCCTG 
AGCTACATCT GCAGATTTCT GCATGAAATC CAGCTGAACT GTGCCGTCAA CAAGATGAGC 
GTGGACAACC TGGCCACTGT GATCGGGGTG AACCTCATCA GGTCGAAGGT TGAAGACCCA 
GCTGTGATTA TGAGAGGGAC TCCTCAGATC CAAAGAGTGA TGACCATGAT GATCAGAGAC 
CACGAAGTCC TCTTCCCCAA GTCTAAGGAT GCACCGATCT CACCCCCTGC CCAGAAAAAC 
GATGCCAAGA AGGCTCCAGT GCCGCGAAGC TCTGTGGGGT GGGATGCCAC GGAAGACCCA 
CCCCTTTCTA GGACAGACAG CTTCAGTAAC ACAGCAAGTA GTCCTGATGC CACCAGTCCC 
ACTGGACCAC TGCCCAGTGA CCAGCATCAG GAAGACAGCG GAAAAGCCCC CAGGGAAAAC 
CCAGGAGACT GGAAGATGCA ATCCCGTAAA AGGACTCAAA CGCTTCCCAA CCGGAAGTGT 
TTCCTGACGT CCGCATTCCA AGGCACCACC AGCAGTAAAC TGGAAATCTT TAAAAATGAG 
TTCTGGTCTC CATCTTCAGA GGCTAAGGCA GGAGAAGGGC ACAGGCGAAC TATGTCCCAA 
GACTTGCGCC ACCTTTCCAA TGACCAGCGG ACTTCTACCT ACGATAATGT CCCCACCTCA 
CCACAGTCCC AAGGGAACCC AGCAGGTGCA CTCTCTCCCC CTGCCAGTGA CTCCAAGAGA 
GATGCTCTTG TTAGCACAGA CTCTGAAATG GAGGCTGGAA GCAAGAACTC TGGCGAGGAT 
GACCTTGATT CTCTGCAGAG GACAGTCCAG AGCCTACAGA AGGAAATAGA AACCCAGAAG 
CAGGTCTATG AGGAACAGAT TAAAAACCTG GAGAAGGAAA ATTACGATGT CTGGGCTAAG 
GTGGTGAGGC TCAATGAAGA ACTCGAGAGG GAGAGGAAGA AATTCGCGGC CCTGGAAATC 
AGCCTTCGAA ATGTGGAGCG CTCCCGGGAG GACGTTGAGA AGAGGAACAG AGTCTTGGAA 
GAAGAAGTCA AGGAGTTTGT GAAGTCGATG GAGAAGCCCA AGACAAAGAC GGATCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273539.1
CDS	441..2120
Translation	MYLPGSTVKEIATNPEEAGKFVFEVIPASSDQNRIGQDSYVLMASSQVEMEEWVKFLRRVAGTPSGAVFGQRLDETVAYEQKFGPHLVPILVEKCAEFILEHGVSEEGIFRLPGQDNLVKQLRDAFDAGERPSFDRDTDVHTVASLLKLYLRDLPEPVVPWSQYEGFLLCGQLMNADEAKAQQELVKQLSTLPRDNYNLLSYICRFLHEIQLNCAVNKMSVDNLATVIGVNLIRSKVEDPAVIMRGTPQIQRVMTMMIRDHEVLFPKSKDAPISPPAQKNDAKKAPVPRSSVGWDATEDPPLSRTDSFSNTASSPDATSPTGPLPSDQHQEDSGKAPRENPGDWKMQSRKRTQTLPNRKCFLTSAFQGTTSSKLEIFKNEFWSPSSEAKAGEGHRRTMSQDLRHLSNDQRTSTYDNVPTSPQSQGNPAGALSPPASDSKRDALVSTDSEMEAGSKNSGEDDLDSLQRTVQSLQKEIETQKQVYEEQIKNLEKENYDVWAKVVRLNEELERERKKFAALEISLRNVERSREDVEKRNRVLEEEVKEFVKSMEKPKTKTDP

Target ORF information:

RefSeq Version	NM_001286610.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho GTPase activating protein 25 (Arhgap25), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286610.1

ORF Insert Sequence:

ATGTATCTGC CAGGCAGTAC AGTCAAAGAA ATTGCCACAA ACCCCGAAGA AGCTGGGAAA 
TTTGTCTTTG AAGTTATTCC AGCCTCAAGT GACCAGAACC GCATTGGACA AGACTCCTAC 
GTCCTCATGG CCAGCTCCCA GGTAGAGATG GAGGAGTGGG TTAAGTTCCT CAGGAGAGTT 
GCTGGCACGC CCTCTGGAGC GGTGTTTGGC CAGCGTCTGG ATGAGACTGT GGCCTATGAG 
CAGAAGTTTG GCCCTCACCT GGTGCCCATC TTGGTGGAGA AGTGCGCTGA GTTCATCCTG 
GAACATGGTG TGAGTGAAGA GGGCATCTTC CGCCTGCCCG GGCAGGACAA CCTTGTGAAG 
CAGCTGAGAG ATGCTTTCGA TGCGGGGGAG CGGCCCTCCT TTGACAGGGA CACAGATGTG 
CACACGGTGG CATCTCTACT AAAGCTCTAC CTCCGAGACC TGCCAGAGCC TGTGGTTCCT 
TGGAGTCAGT ATGAAGGGTT CCTGCTCTGT GGGCAGCTCA TGAACGCAGA TGAGGCAAAG 
GCTCAGCAGG AGTTGGTGAA GCAGCTTTCT ACCCTTCCCC GAGACAACTA CAACCTCCTG 
AGCTACATCT GCAGATTTCT GCATGAAATC CAGCTGAACT GTGCCGTCAA CAAGATGAGC 
GTGGACAACC TGGCCACTGT GATCGGGGTG AACCTCATCA GGTCGAAGGT TGAAGACCCA 
GCTGTGATTA TGAGAGGGAC TCCTCAGATC CAAAGAGTGA TGACCATGAT GATCAGAGAC 
CACGAAGTCC TCTTCCCCAA GTCTAAGGAT GCACCGATCT CACCCCCTGC CCAGAAAAAC 
GATGCCAAGA AGGCTCCAGT GCCGCGAAGC TCTGTGGGGT GGGATGCCAC GGAAGACCCA 
CCCCTTTCTA GGACAGACAG CTTCAGTAAC ACAGCAAGTA GTCCTGATGC CACCAGTCCC 
ACTGGACCAC TGCCCAGTGA CCAGCATCAG GAAGACAGCG GAAAAGCCCC CAGGGAAAAC 
CCAGGAGACT GGAAGATGCA ATCCCGTAAA AGGACTCAAA CGCTTCCCAA CCGGAAGTGT 
TTCCTGACGT CCGCATTCCA AGGCACCACC AGCAGTAAAC TGGAAATCTT TAAAAATGAG 
TTCTGGTCTC CATCTTCAGA GGCTAAGGCA GGAGAAGGGC ACAGGCGAAC TATGTCCCAA 
GACTTGCGCC ACCTTTCCAA TGACCAGCGG ACTTCTACCT ACGATAATGT CCCCACCTCA 
CCACAGTCCC AAGGGAACCC AGCAGGTGCA CTCTCTCCCC CTGCCAGTGA CTCCAAGAGA 
GATGCTCTTG TTAGCACAGA CTCTGAAATG GAGGCTGGAA GCAAGAACTC TGGCGAGGAT 
GACCTTGATT CTCTGCAGAG GACAGTCCAG AGCCTACAGA AGGAAATAGA AACCCAGAAG 
CAGGTCTATG AGGAACAGAT TAAAAACCTG GAGAAGGAAA ATTACGATGT CTGGGCTAAG 
GTGGTGAGGC TCAATGAAGA ACTCGAGAGG GAGAGGAAGA AATTCGCGGC CCTGGAAATC 
AGCCTTCGAA ATGTGGAGCG CTCCCGGGAG GACGTTGAGA AGAGGAACAG AGTCTTGGAA 
GAAGAAGTCA AGGAGTTTGT GAAGTCGATG GAGAAGCCCA AGACAAAGAC GGATCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgap25 ( XM_006505980.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006506043.1 Mus musculus Rho GTPase activating protein 25 (Arhgap25), transcript variant X2, mRNA.

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CloneID	OMu37171
Clone ID Related Accession (Same CDS sequence)	XM_006505980.3
Accession Version	XM_006505980.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rho GTPase-activating protein 25 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006505980.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCACG TCCCCAGGAT GCCGCACTGG GAACTGGGTT GTGGCTTTCC CACCTTGGCG 
CTGCTCTTTT GCTGGGACGT CAGGAAGAGA GCCCCCAAGC TCCGAAAGAA AGCGGTGTTT 
GGCCAGCGTC TGGATGAGAC TGTGGCCTAT GAGCAGAAGT TTGGCCCTCA CCTGGTGCCC 
ATCTTGGTGG AGAAGTGCGC TGAGTTCATC CTGGAACATG GTGTGAGTGA AGAGGGCATC 
TTCCGCCTGC CCGGGCAGGA CAACCTTGTG AAGCAGCTGA GAGATGCTTT CGATGCGGGG 
GAGCGGCCCT CCTTTGACAG GGACACAGAT GTGCACACGG TGGCATCTCT ACTAAAGCTC 
TACCTCCGAG ACCTGCCAGA GCCTGTGGTT CCTTGGAGTC AGTATGAAGG GTTCCTGCTC 
TGTGGGCAGC TCATGAACGC AGATGAGGCA AAGGCTCAGC AGGAGTTGGT GAAGCAGCTT 
TCTACCCTTC CCCGAGACAA CTACAACCTC CTGAGCTACA TCTGCAGATT TCTGCATGAA 
ATCCAGCTGA ACTGTGCCGT CAACAAGATG AGCGTGGACA ACCTGGCCAC TGTGATCGGG 
GTGAACCTCA TCAGGTCGAA GGTTGAAGAC CCAGCTGTGA TTATGAGAGG GACTCCTCAG 
ATCCAAAGAG TGATGACCAT GATGATCAGA GACCACGAAG TCCTCTTCCC CAAGTCTAAG 
GATGCACCGA TCTCACCCCC TGCCCAGAAA AACGATGCCA AGAAGGCTCC AGTGCCGCGA 
AGCTCTGTGG GGTGGGATGC CACGGAAGAC CCACCCCTTT CTAGGACAGA CAGCTTCAGT 
AACACAGCAA GTAGTCCTGA TGCCACCAGT CCCACTGGAC CACTGCCCAG TGACCAGCAT 
CAGGAAGACA GCGGAAAAGC CCCCAGGGAA AACCCAGGAG ACTGGAAGAT GCAATCCCGT 
AAAAGGACTC AAACGCTTCC CAACCGGAAG TGTTTCCTGA CGTCCGCATT CCAAGGCACC 
ACCAGCAGTA AACTGGAAAT CTTTAAAAAT GAGTTCTGGT CTCCATCTTC AGAGGCTAAG 
GCAGGAGAAG GGCACAGGCG AACTATGTCC CAAGACTTGC GCCACCTTTC CAATGACCAG 
CGGACTTCTA CCTACGATAA TGTCCCCACC TCACCACAGT CCCAAGGGAA CCCAGCAGGT 
GCACTCTCTC CCCCTGCCAG TGACTCCAAG AGAGATGCTC TTGTTAGCAC AGACTCTGAA 
ATGGAGGCTG GAAGCAAGAA CTCTGGCGAG GATGACCTTG ATTCTCTGCA GAGGACAGTC 
CAGAGCCTAC AGAAGGAAAT AGAAACCCAG AAGCAGGTCT ATGAGGAACA GATTAAAAAC 
CTGGAGAAGG AAAATTACGA TGTCTGGGCT AAGGTGGTGA GGCTCAATGA AGAACTCGAG 
AGGGAGAGGA AGAAATTCGC GGCCCTGGAA ATCAGCCTTC GAAATGTGGA GCGCTCCCGG 
GAGGACGTTG AGAAGAGGAA CAGAGTCTTG GAAGAAGAAG TCAAGGAGTT TGTGAAGTCG 
ATGGAGAAGC CCAAGACAAA GACGGATCCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006506043.1
CDS	1178..2770
Translation	MPHVPRMPHWELGCGFPTLALLFCWDVRKRAPKLRKKAVFGQRLDETVAYEQKFGPHLVPILVEKCAEFILEHGVSEEGIFRLPGQDNLVKQLRDAFDAGERPSFDRDTDVHTVASLLKLYLRDLPEPVVPWSQYEGFLLCGQLMNADEAKAQQELVKQLSTLPRDNYNLLSYICRFLHEIQLNCAVNKMSVDNLATVIGVNLIRSKVEDPAVIMRGTPQIQRVMTMMIRDHEVLFPKSKDAPISPPAQKNDAKKAPVPRSSVGWDATEDPPLSRTDSFSNTASSPDATSPTGPLPSDQHQEDSGKAPRENPGDWKMQSRKRTQTLPNRKCFLTSAFQGTTSSKLEIFKNEFWSPSSEAKAGEGHRRTMSQDLRHLSNDQRTSTYDNVPTSPQSQGNPAGALSPPASDSKRDALVSTDSEMEAGSKNSGEDDLDSLQRTVQSLQKEIETQKQVYEEQIKNLEKENYDVWAKVVRLNEELERERKKFAALEISLRNVERSREDVEKRNRVLEEEVKEFVKSMEKPKTKTDP

Target ORF information:

RefSeq Version	XM_006505980.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho GTPase activating protein 25 (Arhgap25), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006505980.3

ORF Insert Sequence:

ATGCCTCACG TCCCCAGGAT GCCGCACTGG GAACTGGGTT GTGGCTTTCC CACCTTGGCG 
CTGCTCTTTT GCTGGGACGT CAGGAAGAGA GCCCCCAAGC TCCGAAAGAA AGCGGTGTTT 
GGCCAGCGTC TGGATGAGAC TGTGGCCTAT GAGCAGAAGT TTGGCCCTCA CCTGGTGCCC 
ATCTTGGTGG AGAAGTGCGC TGAGTTCATC CTGGAACATG GTGTGAGTGA AGAGGGCATC 
TTCCGCCTGC CCGGGCAGGA CAACCTTGTG AAGCAGCTGA GAGATGCTTT CGATGCGGGG 
GAGCGGCCCT CCTTTGACAG GGACACAGAT GTGCACACGG TGGCATCTCT ACTAAAGCTC 
TACCTCCGAG ACCTGCCAGA GCCTGTGGTT CCTTGGAGTC AGTATGAAGG GTTCCTGCTC 
TGTGGGCAGC TCATGAACGC AGATGAGGCA AAGGCTCAGC AGGAGTTGGT GAAGCAGCTT 
TCTACCCTTC CCCGAGACAA CTACAACCTC CTGAGCTACA TCTGCAGATT TCTGCATGAA 
ATCCAGCTGA ACTGTGCCGT CAACAAGATG AGCGTGGACA ACCTGGCCAC TGTGATCGGG 
GTGAACCTCA TCAGGTCGAA GGTTGAAGAC CCAGCTGTGA TTATGAGAGG GACTCCTCAG 
ATCCAAAGAG TGATGACCAT GATGATCAGA GACCACGAAG TCCTCTTCCC CAAGTCTAAG 
GATGCACCGA TCTCACCCCC TGCCCAGAAA AACGATGCCA AGAAGGCTCC AGTGCCGCGA 
AGCTCTGTGG GGTGGGATGC CACGGAAGAC CCACCCCTTT CTAGGACAGA CAGCTTCAGT 
AACACAGCAA GTAGTCCTGA TGCCACCAGT CCCACTGGAC CACTGCCCAG TGACCAGCAT 
CAGGAAGACA GCGGAAAAGC CCCCAGGGAA AACCCAGGAG ACTGGAAGAT GCAATCCCGT 
AAAAGGACTC AAACGCTTCC CAACCGGAAG TGTTTCCTGA CGTCCGCATT CCAAGGCACC 
ACCAGCAGTA AACTGGAAAT CTTTAAAAAT GAGTTCTGGT CTCCATCTTC AGAGGCTAAG 
GCAGGAGAAG GGCACAGGCG AACTATGTCC CAAGACTTGC GCCACCTTTC CAATGACCAG 
CGGACTTCTA CCTACGATAA TGTCCCCACC TCACCACAGT CCCAAGGGAA CCCAGCAGGT 
GCACTCTCTC CCCCTGCCAG TGACTCCAAG AGAGATGCTC TTGTTAGCAC AGACTCTGAA 
ATGGAGGCTG GAAGCAAGAA CTCTGGCGAG GATGACCTTG ATTCTCTGCA GAGGACAGTC 
CAGAGCCTAC AGAAGGAAAT AGAAACCCAG AAGCAGGTCT ATGAGGAACA GATTAAAAAC 
CTGGAGAAGG AAAATTACGA TGTCTGGGCT AAGGTGGTGA GGCTCAATGA AGAACTCGAG 
AGGGAGAGGA AGAAATTCGC GGCCCTGGAA ATCAGCCTTC GAAATGTGGA GCGCTCCCGG 
GAGGACGTTG AGAAGAGGAA CAGAGTCTTG GAAGAAGAAG TCAAGGAGTT TGTGAAGTCG 
ATGGAGAAGC CCAAGACAAA GACGGATCCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgap25 ( XM_017321530.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017177019.1 Mus musculus Rho GTPase activating protein 25 (Arhgap25), transcript variant X1, mRNA.

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CloneID	OMu81697
Clone ID Related Accession (Same CDS sequence)	XM_017321530.1
Accession Version	XM_017321530.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2202bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	rho GTPase-activating protein 25 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCTGTT GGAGAGACCC TGTTGGGCAG AGAGATCCAA GCTACAGGCT GGCTTCCCAG 
AATGGCTATA GAGGAAAGCC AGGCACTTTC TGGCGGTCTC TGCAGCTGTG TGGACCTTAC 
TCTAAGCACA GAGCGGGGCA CTATTTTGTC CCCTGTCAGA TACTCCCACG CAGGAAAAGG 
AAGCACCTTC CGTCAGATGC TGTGGCCACT TCCTCTTCAG AGGCTCCAGC CAATCTCCGC 
TGTGCAGCAG GCAGGGCCGG AGGCAGCTGG ACGGCAGGAG CTGGTCGTCA GTTGGGCTCC 
TTTCAGGAGC AGGACCCTGT GAGGTGTGGG GGACAACAGG GTTTCCTCTC AGCCTCCCCC 
ACCCTTACCC CCTCTCTCTT GACTTTGTGC TTCATTTCCC TCGGTCAGCC CGCCTATCTG 
TTCCTCTCCT TAGCTCGGTC GAGGAGTGTG ATGACCGGGG AGCAGATGGC GGCCTTTCAC 
CCACCCACCA CTCCCAACCC CCTGGAAAGG CCAATTAAGG TGGGCTGGCT GAAGAAGCAG 
AGGTCCATCG TGAAAAACTG GCAGCAGCGG TACTTTGTGT TGAGAGCTCA GCAACTCTAC 
TACTACAAGG ACGAAGAGGA CTCAAAGCCA CAGGGCTGCA TGTATCTGCC AGGCAGTACA 
GTCAAAGAAA TTGCCACAAA CCCCGAAGAA GCTGGGAAAT TTGTCTTTGA AGTTATTCCA 
GCGGTGTTTG GCCAGCGTCT GGATGAGACT GTGGCCTATG AGCAGAAGTT TGGCCCTCAC 
CTGGTGCCCA TCTTGGTGGA GAAGTGCGCT GAGTTCATCC TGGAACATGG TGTGAGTGAA 
GAGGGCATCT TCCGCCTGCC CGGGCAGGAC AACCTTGTGA AGCAGCTGAG AGATGCTTTC 
GATGCGGGGG AGCGGCCCTC CTTTGACAGG GACACAGATG TGCACACGGT GGCATCTCTA 
CTAAAGCTCT ACCTCCGAGA CCTGCCAGAG CCTGTGGTTC CTTGGAGTCA GTATGAAGGG 
TTCCTGCTCT GTGGGCAGCT CATGAACGCA GATGAGGCAA AGGCTCAGCA GGAGTTGGTG 
AAGCAGCTTT CTACCCTTCC CCGAGACAAC TACAACCTCC TGAGCTACAT CTGCAGATTT 
CTGCATGAAA TCCAGCTGAA CTGTGCCGTC AACAAGATGA GCGTGGACAA CCTGGCCACT 
GTGATCGGGG TGAACCTCAT CAGGTCGAAG GTTGAAGACC CAGCTGTGAT TATGAGAGGG 
ACTCCTCAGA TCCAAAGAGT GATGACCATG ATGATCAGAG ACCACGAAGT CCTCTTCCCC 
AAGTCTAAGG ATGCACCGAT CTCACCCCCT GCCCAGAAAA ACGATGCCAA GAAGGCTCCA 
GTGCCGCGAA GCTCTGTGGG GTGGGATGCC ACGGAAGACC CACCCCTTTC TAGGACAGAC 
AGCTTCAGTA ACACAGCAAG TAGTCCTGAT GCCACCAGTC CCACTGGACC ACTGCCCAGT 
GACCAGCATC AGGAAGACAG CGGAAAAGCC CCCAGGGAAA ACCCAGGAGA CTGGAAGATG 
CAATCCCGTA AAAGGACTCA AACGCTTCCC AACCGGAAGT GTTTCCTGAC GTCCGCATTC 
CAAGGCACCA CCAGCAGTAA ACTGGAAATC TTTAAAAATG AGTTCTGGTC TCCATCTTCA 
GAGGCTAAGG CAGGAGAAGG GCACAGGCGA ACTATGTCCC AAGACTTGCG CCACCTTTCC 
AATGACCAGC GGACTTCTAC CTACGATAAT GTCCCCACCT CACCACAGTC CCAAGGGAAC 
CCAGCAGGTG CACTCTCTCC CCCTGCCAGT GACTCCAAGA GAGATGCTCT TGTTAGCACA 
GACTCTGAAA TGGAGGCTGG AAGCAAGAAC TCTGGCGAGG ATGACCTTGA TTCTCTGCAG 
AGGACAGTCC AGAGCCTACA GAAGGAAATA GAAACCCAGA AGCAGGTCTA TGAGGAACAG 
ATTAAAAACC TGGAGAAGGA AAATTACGAT GTCTGGGCTA AGGTGGTGAG GCTCAATGAA 
GAACTCGAGA GGGAGAGGAA GAAATTCGCG GCCCTGGAAA TCAGCCTTCG AAATGTGGAG 
CGCTCCCGGG AGGACGTTGA GAAGAGGAAC AGAGTCTTGG AAGAAGAAGT CAAGGAGTTT 
GTGAAGTCGA TGGAGAAGCC CAAGACAAAG ACGGATCCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017177019.1
CDS	98..2299
Translation	MICWRDPVGQRDPSYRLASQNGYRGKPGTFWRSLQLCGPYSKHRAGHYFVPCQILPRRKRKHLPSDAVATSSSEAPANLRCAAGRAGGSWTAGAGRQLGSFQEQDPVRCGGQQGFLSASPTLTPSLLTLCFISLGQPAYLFLSLARSRSVMTGEQMAAFHPPTTPNPLERPIKVGWLKKQRSIVKNWQQRYFVLRAQQLYYYKDEEDSKPQGCMYLPGSTVKEIATNPEEAGKFVFEVIPAVFGQRLDETVAYEQKFGPHLVPILVEKCAEFILEHGVSEEGIFRLPGQDNLVKQLRDAFDAGERPSFDRDTDVHTVASLLKLYLRDLPEPVVPWSQYEGFLLCGQLMNADEAKAQQELVKQLSTLPRDNYNLLSYICRFLHEIQLNCAVNKMSVDNLATVIGVNLIRSKVEDPAVIMRGTPQIQRVMTMMIRDHEVLFPKSKDAPISPPAQKNDAKKAPVPRSSVGWDATEDPPLSRTDSFSNTASSPDATSPTGPLPSDQHQEDSGKAPRENPGDWKMQSRKRTQTLPNRKCFLTSAFQGTTSSKLEIFKNEFWSPSSEAKAGEGHRRTMSQDLRHLSNDQRTSTYDNVPTSPQSQGNPAGALSPPASDSKRDALVSTDSEMEAGSKNSGEDDLDSLQRTVQSLQKEIETQKQVYEEQIKNLEKENYDVWAKVVRLNEELERERKKFAALEISLRNVERSREDVEKRNRVLEEEVKEFVKSMEKPKTKTDP

Target ORF information:

RefSeq Version	XM_017321530.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho GTPase activating protein 25 (Arhgap25), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017321530.1

ORF Insert Sequence:

ATGATCTGTT GGAGAGACCC TGTTGGGCAG AGAGATCCAA GCTACAGGCT GGCTTCCCAG 
AATGGCTATA GAGGAAAGCC AGGCACTTTC TGGCGGTCTC TGCAGCTGTG TGGACCTTAC 
TCTAAGCACA GAGCGGGGCA CTATTTTGTC CCCTGTCAGA TACTCCCACG CAGGAAAAGG 
AAGCACCTTC CGTCAGATGC TGTGGCCACT TCCTCTTCAG AGGCTCCAGC CAATCTCCGC 
TGTGCAGCAG GCAGGGCCGG AGGCAGCTGG ACGGCAGGAG CTGGTCGTCA GTTGGGCTCC 
TTTCAGGAGC AGGACCCTGT GAGGTGTGGG GGACAACAGG GTTTCCTCTC AGCCTCCCCC 
ACCCTTACCC CCTCTCTCTT GACTTTGTGC TTCATTTCCC TCGGTCAGCC CGCCTATCTG 
TTCCTCTCCT TAGCTCGGTC GAGGAGTGTG ATGACCGGGG AGCAGATGGC GGCCTTTCAC 
CCACCCACCA CTCCCAACCC CCTGGAAAGG CCAATTAAGG TGGGCTGGCT GAAGAAGCAG 
AGGTCCATCG TGAAAAACTG GCAGCAGCGG TACTTTGTGT TGAGAGCTCA GCAACTCTAC 
TACTACAAGG ACGAAGAGGA CTCAAAGCCA CAGGGCTGCA TGTATCTGCC AGGCAGTACA 
GTCAAAGAAA TTGCCACAAA CCCCGAAGAA GCTGGGAAAT TTGTCTTTGA AGTTATTCCA 
GCGGTGTTTG GCCAGCGTCT GGATGAGACT GTGGCCTATG AGCAGAAGTT TGGCCCTCAC 
CTGGTGCCCA TCTTGGTGGA GAAGTGCGCT GAGTTCATCC TGGAACATGG TGTGAGTGAA 
GAGGGCATCT TCCGCCTGCC CGGGCAGGAC AACCTTGTGA AGCAGCTGAG AGATGCTTTC 
GATGCGGGGG AGCGGCCCTC CTTTGACAGG GACACAGATG TGCACACGGT GGCATCTCTA 
CTAAAGCTCT ACCTCCGAGA CCTGCCAGAG CCTGTGGTTC CTTGGAGTCA GTATGAAGGG 
TTCCTGCTCT GTGGGCAGCT CATGAACGCA GATGAGGCAA AGGCTCAGCA GGAGTTGGTG 
AAGCAGCTTT CTACCCTTCC CCGAGACAAC TACAACCTCC TGAGCTACAT CTGCAGATTT 
CTGCATGAAA TCCAGCTGAA CTGTGCCGTC AACAAGATGA GCGTGGACAA CCTGGCCACT 
GTGATCGGGG TGAACCTCAT CAGGTCGAAG GTTGAAGACC CAGCTGTGAT TATGAGAGGG 
ACTCCTCAGA TCCAAAGAGT GATGACCATG ATGATCAGAG ACCACGAAGT CCTCTTCCCC 
AAGTCTAAGG ATGCACCGAT CTCACCCCCT GCCCAGAAAA ACGATGCCAA GAAGGCTCCA 
GTGCCGCGAA GCTCTGTGGG GTGGGATGCC ACGGAAGACC CACCCCTTTC TAGGACAGAC 
AGCTTCAGTA ACACAGCAAG TAGTCCTGAT GCCACCAGTC CCACTGGACC ACTGCCCAGT 
GACCAGCATC AGGAAGACAG CGGAAAAGCC CCCAGGGAAA ACCCAGGAGA CTGGAAGATG 
CAATCCCGTA AAAGGACTCA AACGCTTCCC AACCGGAAGT GTTTCCTGAC GTCCGCATTC 
CAAGGCACCA CCAGCAGTAA ACTGGAAATC TTTAAAAATG AGTTCTGGTC TCCATCTTCA 
GAGGCTAAGG CAGGAGAAGG GCACAGGCGA ACTATGTCCC AAGACTTGCG CCACCTTTCC 
AATGACCAGC GGACTTCTAC CTACGATAAT GTCCCCACCT CACCACAGTC CCAAGGGAAC 
CCAGCAGGTG CACTCTCTCC CCCTGCCAGT GACTCCAAGA GAGATGCTCT TGTTAGCACA 
GACTCTGAAA TGGAGGCTGG AAGCAAGAAC TCTGGCGAGG ATGACCTTGA TTCTCTGCAG 
AGGACAGTCC AGAGCCTACA GAAGGAAATA GAAACCCAGA AGCAGGTCTA TGAGGAACAG 
ATTAAAAACC TGGAGAAGGA AAATTACGAT GTCTGGGCTA AGGTGGTGAG GCTCAATGAA 
GAACTCGAGA GGGAGAGGAA GAAATTCGCG GCCCTGGAAA TCAGCCTTCG AAATGTGGAG 
CGCTCCCGGG AGGACGTTGA GAAGAGGAAC AGAGTCTTGG AAGAAGAAGT CAAGGAGTTT 
GTGAAGTCGA TGGAGAAGCC CAAGACAAAG ACGGATCCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arhgap25 ( XM_017321531.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017177020.1 Mus musculus Rho GTPase activating protein 25 (Arhgap25), transcript variant X1, mRNA.

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CloneID	OMu81698
Clone ID Related Accession (Same CDS sequence)	XM_017321531.1
Accession Version	XM_017321531.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	rho GTPase-activating protein 25 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTCA AGCTGCCAAG GAACTGGGAT TTCAATTTGA AAGCGGAGGC TTCAAAAATA 
GCTCGGTCGA GGAGTGTGAT GACCGGGGAG CAGATGGCGG CCTTTCACCC ACCCACCACT 
CCCAACCCCC TGGAAAGGCC AATTAAGGTG GGCTGGCTGA AGAAGCAGAG GTCCATCGTG 
AAAAACTGGC AGCAGCGGTA CTTTGTGTTG AGAGCTCAGC AACTCTACTA CTACAAGGAC 
GAAGAGGACT CAAAGCCACA GGGCTGCATG TATCTGCCAG GCAGTACAGT CAAAGAAATT 
GCCACAAACC CCGAAGAAGC TGGGAAATTT GTCTTTGAAG TTATTCCAGC GGTGTTTGGC 
CAGCGTCTGG ATGAGACTGT GGCCTATGAG CAGAAGTTTG GCCCTCACCT GGTGCCCATC 
TTGGTGGAGA AGTGCGCTGA GTTCATCCTG GAACATGGTG TGAGTGAAGA GGGCATCTTC 
CGCCTGCCCG GGCAGGACAA CCTTGTGAAG CAGCTGAGAG ATGCTTTCGA TGCGGGGGAG 
CGGCCCTCCT TTGACAGGGA CACAGATGTG CACACGGTGG CATCTCTACT AAAGCTCTAC 
CTCCGAGACC TGCCAGAGCC TGTGGTTCCT TGGAGTCAGT ATGAAGGGTT CCTGCTCTGT 
GGGCAGCTCA TGAACGCAGA TGAGGCAAAG GCTCAGCAGG AGTTGGTGAA GCAGCTTTCT 
ACCCTTCCCC GAGACAACTA CAACCTCCTG AGCTACATCT GCAGATTTCT GCATGAAATC 
CAGCTGAACT GTGCCGTCAA CAAGATGAGC GTGGACAACC TGGCCACTGT GATCGGGGTG 
AACCTCATCA GGTCGAAGGT TGAAGACCCA GCTGTGATTA TGAGAGGGAC TCCTCAGATC 
CAAAGAGTGA TGACCATGAT GATCAGAGAC CACGAAGTCC TCTTCCCCAA GTCTAAGGAT 
GCACCGATCT CACCCCCTGC CCAGAAAAAC GATGCCAAGA AGGCTCCAGT GCCGCGAAGC 
TCTGTGGGGT GGGATGCCAC GGAAGACCCA CCCCTTTCTA GGACAGACAG CTTCAGTAAC 
ACAGCAAGTA GTCCTGATGC CACCAGTCCC ACTGGACCAC TGCCCAGTGA CCAGCATCAG 
GAAGACAGCG GAAAAGCCCC CAGGGAAAAC CCAGGAGACT GGAAGATGCA ATCCCGTAAA 
AGGACTCAAA CGCTTCCCAA CCGGAAGTGT TTCCTGACGT CCGCATTCCA AGGCACCACC 
AGCAGTAAAC TGGAAATCTT TAAAAATGAG TTCTGGTCTC CATCTTCAGA GGCTAAGGCA 
GGAGAAGGGC ACAGGCGAAC TATGTCCCAA GACTTGCGCC ACCTTTCCAA TGACCAGCGG 
ACTTCTACCT ACGATAATGT CCCCACCTCA CCACAGTCCC AAGGGAACCC AGCAGGTGCA 
CTCTCTCCCC CTGCCAGTGA CTCCAAGAGA GATGCTCTTG TTAGCACAGA CTCTGAAATG 
GAGGCTGGAA GCAAGAACTC TGGCGAGGAT GACCTTGATT CTCTGCAGAG GACAGTCCAG 
AGCCTACAGA AGGAAATAGA AACCCAGAAG CAGGTCTATG AGGAACAGAT TAAAAACCTG 
GAGAAGGAAA ATTACGATGT CTGGGCTAAG GTGGTGAGGC TCAATGAAGA ACTCGAGAGG 
GAGAGGAAGA AATTCGCGGC CCTGGAAATC AGCCTTCGAA ATGTGGAGCG CTCCCGGGAG 
GACGTTGAGA AGAGGAACAG AGTCTTGGAA GAAGAAGTCA AGGAGTTTGT GAAGTCGATG 
GAGAAGCCCA AGACAAAGAC GGATCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017177020.1
CDS	376..2205
Translation	MSLKLPRNWDFNLKAEASKIARSRSVMTGEQMAAFHPPTTPNPLERPIKVGWLKKQRSIVKNWQQRYFVLRAQQLYYYKDEEDSKPQGCMYLPGSTVKEIATNPEEAGKFVFEVIPAVFGQRLDETVAYEQKFGPHLVPILVEKCAEFILEHGVSEEGIFRLPGQDNLVKQLRDAFDAGERPSFDRDTDVHTVASLLKLYLRDLPEPVVPWSQYEGFLLCGQLMNADEAKAQQELVKQLSTLPRDNYNLLSYICRFLHEIQLNCAVNKMSVDNLATVIGVNLIRSKVEDPAVIMRGTPQIQRVMTMMIRDHEVLFPKSKDAPISPPAQKNDAKKAPVPRSSVGWDATEDPPLSRTDSFSNTASSPDATSPTGPLPSDQHQEDSGKAPRENPGDWKMQSRKRTQTLPNRKCFLTSAFQGTTSSKLEIFKNEFWSPSSEAKAGEGHRRTMSQDLRHLSNDQRTSTYDNVPTSPQSQGNPAGALSPPASDSKRDALVSTDSEMEAGSKNSGEDDLDSLQRTVQSLQKEIETQKQVYEEQIKNLEKENYDVWAKVVRLNEELERERKKFAALEISLRNVERSREDVEKRNRVLEEEVKEFVKSMEKPKTKTDP

Target ORF information:

RefSeq Version	XM_017321531.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Rho GTPase activating protein 25 (Arhgap25), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017321531.1

ORF Insert Sequence:

ATGTCTCTCA AGCTGCCAAG GAACTGGGAT TTCAATTTGA AAGCGGAGGC TTCAAAAATA 
GCTCGGTCGA GGAGTGTGAT GACCGGGGAG CAGATGGCGG CCTTTCACCC ACCCACCACT 
CCCAACCCCC TGGAAAGGCC AATTAAGGTG GGCTGGCTGA AGAAGCAGAG GTCCATCGTG 
AAAAACTGGC AGCAGCGGTA CTTTGTGTTG AGAGCTCAGC AACTCTACTA CTACAAGGAC 
GAAGAGGACT CAAAGCCACA GGGCTGCATG TATCTGCCAG GCAGTACAGT CAAAGAAATT 
GCCACAAACC CCGAAGAAGC TGGGAAATTT GTCTTTGAAG TTATTCCAGC GGTGTTTGGC 
CAGCGTCTGG ATGAGACTGT GGCCTATGAG CAGAAGTTTG GCCCTCACCT GGTGCCCATC 
TTGGTGGAGA AGTGCGCTGA GTTCATCCTG GAACATGGTG TGAGTGAAGA GGGCATCTTC 
CGCCTGCCCG GGCAGGACAA CCTTGTGAAG CAGCTGAGAG ATGCTTTCGA TGCGGGGGAG 
CGGCCCTCCT TTGACAGGGA CACAGATGTG CACACGGTGG CATCTCTACT AAAGCTCTAC 
CTCCGAGACC TGCCAGAGCC TGTGGTTCCT TGGAGTCAGT ATGAAGGGTT CCTGCTCTGT 
GGGCAGCTCA TGAACGCAGA TGAGGCAAAG GCTCAGCAGG AGTTGGTGAA GCAGCTTTCT 
ACCCTTCCCC GAGACAACTA CAACCTCCTG AGCTACATCT GCAGATTTCT GCATGAAATC 
CAGCTGAACT GTGCCGTCAA CAAGATGAGC GTGGACAACC TGGCCACTGT GATCGGGGTG 
AACCTCATCA GGTCGAAGGT TGAAGACCCA GCTGTGATTA TGAGAGGGAC TCCTCAGATC 
CAAAGAGTGA TGACCATGAT GATCAGAGAC CACGAAGTCC TCTTCCCCAA GTCTAAGGAT 
GCACCGATCT CACCCCCTGC CCAGAAAAAC GATGCCAAGA AGGCTCCAGT GCCGCGAAGC 
TCTGTGGGGT GGGATGCCAC GGAAGACCCA CCCCTTTCTA GGACAGACAG CTTCAGTAAC 
ACAGCAAGTA GTCCTGATGC CACCAGTCCC ACTGGACCAC TGCCCAGTGA CCAGCATCAG 
GAAGACAGCG GAAAAGCCCC CAGGGAAAAC CCAGGAGACT GGAAGATGCA ATCCCGTAAA 
AGGACTCAAA CGCTTCCCAA CCGGAAGTGT TTCCTGACGT CCGCATTCCA AGGCACCACC 
AGCAGTAAAC TGGAAATCTT TAAAAATGAG TTCTGGTCTC CATCTTCAGA GGCTAAGGCA 
GGAGAAGGGC ACAGGCGAAC TATGTCCCAA GACTTGCGCC ACCTTTCCAA TGACCAGCGG 
ACTTCTACCT ACGATAATGT CCCCACCTCA CCACAGTCCC AAGGGAACCC AGCAGGTGCA 
CTCTCTCCCC CTGCCAGTGA CTCCAAGAGA GATGCTCTTG TTAGCACAGA CTCTGAAATG 
GAGGCTGGAA GCAAGAACTC TGGCGAGGAT GACCTTGATT CTCTGCAGAG GACAGTCCAG 
AGCCTACAGA AGGAAATAGA AACCCAGAAG CAGGTCTATG AGGAACAGAT TAAAAACCTG 
GAGAAGGAAA ATTACGATGT CTGGGCTAAG GTGGTGAGGC TCAATGAAGA ACTCGAGAGG 
GAGAGGAAGA AATTCGCGGC CCTGGAAATC AGCCTTCGAA ATGTGGAGCG CTCCCGGGAG 
GACGTTGAGA AGAGGAACAG AGTCTTGGAA GAAGAAGTCA AGGAGTTTGT GAAGTCGATG 
GAGAAGCCCA AGACAAAGAC GGATCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Rac GTPase Activating Protein ARHGAP25 Regulates Leukocyte Transendothelial Migration in Mice.
Journal of immunology (Baltimore, Md. : 1950)197(7)2807-15(2016 Oct)
Cs?p?nyi-K?mi R,Wisniewski ?,Bartos B,L?vai P,N?meth T,Bal?zs B,Kurz AR,Bierschenk S,Sperandio M,Ligeti E

Phosphoproteomic profiling of mouse primary HSPCs reveals new regulators of HSPC mobilization.
Blood128(11)1465-74(2016 09)
Wang LD,Ficarro SB,Hutchinson JN,Csepanyi-Komi R,Nguyen PT,Wisniewski E,Sullivan J,Hofmann O,Ligeti E,Marto JA,Wagers AJ

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Neuron83(2)309-323(2014 Jul)
Thompson CL,Ng L,Menon V,Martinez S,Lee CK,Glattfelder K,Sunkin SM,Henry A,Lau C,Dang C,Garcia-Lopez R,Martinez-Ferre A,Pombero A,Rubenstein JLR,Wakeman WB,Hohmann J,Dee N,Sodt AJ,Young R,Smith K,Nguyen TN,Kidney J,Kuan L,Jeromin A,Kaykas A,Miller J,Page D,Orta G,Bernard A,Riley Z,Smith S,Wohnoutka P,Hawrylycz MJ,Puelles L,Jones AR

Identification and characterization of ARHGAP24 and ARHGAP25 genes in silico.
International journal of molecular medicine14(2)333-8(2004 Aug)
Katoh M,Katoh M

Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
DNA research : an international journal for rapid publication of reports on genes and genomes11(3)205-18(2004 Jun)
Okazaki N,F-Kikuno R,Ohara R,Inamoto S,Koseki H,Hiraoka S,Saga Y,Seino S,Nishimura M,Kaisho T,Hoshino K,Kitamura H,Nagase T,Ohara O,Koga H

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Arhgap25 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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