Ajap1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ajap1
Entrez Gene ID	230959
Full Name	adherens junction associated protein 1
Synonyms	Gm573
General protein information	Preferred Names adherens junction associated protein 1 Names adherens junction-associated protein 1 likely ortholog of H. sapiens transmembrane protein SHREW1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 E2

mRNA and Protein(s)

mRNA	Protein	Name
XM_006538824.4	XP_006538887.1	adherens junction-associated protein 1 isoform X1
NM_001099299.1	NP_001092769.1	adherens junction-associated protein 1
XM_006538824.3	XP_006538887.1	adherens junction-associated protein 1 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	AJAP1	XP_001153509.1
Canis lupus familiaris (dog)	AJAP1	XP_005620500.1
Homo sapiens (human)	AJAP1	NP_001035943.1
Bos taurus (cattle)	AJAP1	NP_001193744.1
Mus musculus (house mouse)	Ajap1	NP_001092769.1
Rattus norvegicus (Norway rat)	Ajap1	NP_001094484.1
Danio rerio (zebrafish)	LOC101887016	XP_005167007.1
Gallus gallus (chicken)	AJAP1	XP_004947415.1
Xenopus tropicalis (tropical clawed frog)	ajap1	NP_001072363.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Tbx5 is required for avian and Mammalian epicardial formation and coronary vasculogenesis.
Diman NY, Brooks G, Kruithof BP, Elemento O, Seidman JG, Seidman CE, Basson CT, Hatcher CJ
Circulation research115(10)834-44(2014 Oct)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

Junction protein shrew-1 influences cell invasion and interacts with invasion-promoting protein CD147.
Schreiner A, Ruonala M, Jakob V, Suthaus J, Boles E, Wouters F, Starzinski-Powitz A
Molecular biology of the cell18(4)1272-81(2007 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ajap1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ajap1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu21342	NM_001099299.1 Latest version!	Mus musculus adherens junction associated protein 1 (Ajap1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu34175	XM_006538824.4 Latest version!	Mus musculus adherens junction associated protein 1 (Ajap1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OMu34175	XM_006538824.3	Mus musculus adherens junction associated protein 1 (Ajap1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ajap1 ( NM_001099299.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001092769.1 Mus musculus adherens junction associated protein 1 (Ajap1), mRNA.

Price & Availability↑

CloneID	OMu21342
Clone ID Related Accession (Same CDS sequence)	NM_001099299.1
Accession Version	NM_001099299.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	adherens junction-associated protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL954390.19 and AL805910.7. On or before Jul 25, 2007 this sequence version replaced XM_001475023.1, XM_485506.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. CCDS Note: This CCDS ID represents the mouse ortholog of the protein described in PMIDs: 14595118 and 17267690. This variant is supported by BC147230.1 and BE648223.1. It should be noted that this transcript is predicted to undergo nonsense-mediated mRNA decay (NMD). However, the protein is represented because it was detected endogenously in human in PMID: 14595118. It is likely that the majority of transcripts representing this variant will undergo NMD, while some low level of NMD escape may allow for the expression of this protein. ##Evidence-Data-START## CDS exon combination :: BC147658.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164139 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## NMD candidate :: translation inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTGGATCC AACAGCTTTT AGGACTCAGC TCCATGTCTA TCCGCTGGCC CGGCCGCTCT 
CTCGGAAGCC ATGCCTGGAT ACTGATAGCC ATGCTGCAGC TTGCGGTCGA CTTCCCATCC 
TGTGACTCAC TAGGCCCTGG GCCCGAGTTC CGGCTCCTGT CACGCCCACA GAGGCCCCAG 
CGGCTGTGGA GTCTGCGAAG TGGACCCCCA ACGAGGCTAC CGACACCGGC CTGGAGTCCC 
CGTGCTGCCC GGGCAGAACG TGCCCACGGA CCCATACAGA TGCAGACTCC CCGTGCTCGG 
AGGGCCCACA GGCCCAGGGA CCAGGTAGCC ACCCTCGGAC CCAAAGGGGG ACTCACCAAG 
CCCCCAGCTG CCACCAGGTC GAGCCCTTCC CTCGCCTCTG CGACGGCCTC ATCCTCCATA 
GTGACTGCTG GGGCTGCAGA GCACCAGGGC CTCCTAAGAC GGGGTAGGAG GCACACACAT 
GATACAGAGT TCAACGATTT CGACTTCCGT GGTGGCAGGC CCACGACAGA GACAGAATTC 
ATAGCCTGGG GGCCTACGGG GGATGAGGAC GCCCTGGAGT CCAACACCTT CCCTGGTGGT 
TTCGGCCCCA CCACGGTCTC CATCTTGCAA ACACGGAAGA CAACGGTGGC CACCACCACC 
ACCACCACCG CGGCCAGCAC GGCCACCGCC ATGACACTGC AGACTAAGGG GGTCACCGAG 
TCCCTGGATC CCTGGAAAAG GACCCCTGTT GGGGTTAGCA CAACGGAGCC TTCCACTAGT 
CCCAGCAGCA ATGGGAAAGA CATCCAGCCC CCGAGGATCT TAGGGGAGAC CTCAGGTCTG 
GCTGTGCATC AGATCATCAC CATCACCGTC TCCCTCATCA TGGTCATCGC TGCGCTCATC 
ACAACTCTTG TCTTAAAAAA TTGCTGTGCC CCAAGCGGGC ACACTCGGAG GAATAGCCAC 
CAGCGGAAGA TGAATCAGCA GGAGGAGAGT TGCCAGAACT TGACGGACTT CACCCCGGCC 
CGGGTGCCCA GCAGTGTGGA CATCTTCACC GCCTATAATG AGACACTGCA GTGTTCTCAC 
GAGTGTGTCA GGGCCTCTGT GCCCGTGTAT GCCGACGAGA CGCTGCACTC GACCGGGGAG 
TACAAGTCCA CATTCAACGG AAACCGAACA TCCTCTGCGG ATAGGCATCT CATCCCTGTG 
GCCTTTGTGT CTGAGAAATG GTTTGAAATC TCCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001092769.1
CDS	674..1912
Translation	MWIQQLLGLSSMSIRWPGRSLGSHAWILIAMLQLAVDFPSCDSLGPGPEFRLLSRPQRPQRLWSLRSGPPTRLPTPAWSPRAARAERAHGPIQMQTPRARRAHRPRDQVATLGPKGGLTKPPAATRSSPSLASATASSSIVTAGAAEHQGLLRRGRRHTHDTEFNDFDFRGGRPTTETEFIAWGPTGDEDALESNTFPGGFGPTTVSILQTRKTTVATTTTTTAASTATAMTLQTKGVTESLDPWKRTPVGVSTTEPSTSPSSNGKDIQPPRILGETSGLAVHQIITITVSLIMVIAALITTLVLKNCCAPSGHTRRNSHQRKMNQQEESCQNLTDFTPARVPSSVDIFTAYNETLQCSHECVRASVPVYADETLHSTGEYKSTFNGNRTSSADRHLIPVAFVSEKWFEISC

Target ORF information:

RefSeq Version	NM_001099299.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus adherens junction associated protein 1 (Ajap1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001099299.1

ORF Insert Sequence:

ATGTGGATCC AACAGCTTTT AGGACTCAGC TCCATGTCTA TCCGCTGGCC CGGCCGCTCT 
CTCGGAAGCC ATGCCTGGAT ACTGATAGCC ATGCTGCAGC TTGCGGTCGA CTTCCCATCC 
TGTGACTCAC TAGGCCCTGG GCCCGAGTTC CGGCTCCTGT CACGCCCACA GAGGCCCCAG 
CGGCTGTGGA GTCTGCGAAG TGGACCCCCA ACGAGGCTAC CGACACCGGC CTGGAGTCCC 
CGTGCTGCCC GGGCAGAACG TGCCCACGGA CCCATACAGA TGCAGACTCC CCGTGCTCGG 
AGGGCCCACA GGCCCAGGGA CCAGGTAGCC ACCCTCGGAC CCAAAGGGGG ACTCACCAAG 
CCCCCAGCTG CCACCAGGTC GAGCCCTTCC CTCGCCTCTG CGACGGCCTC ATCCTCCATA 
GTGACTGCTG GGGCTGCAGA GCACCAGGGC CTCCTAAGAC GGGGTAGGAG GCACACACAT 
GATACAGAGT TCAACGATTT CGACTTCCGT GGTGGCAGGC CCACGACAGA GACAGAATTC 
ATAGCCTGGG GGCCTACGGG GGATGAGGAC GCCCTGGAGT CCAACACCTT CCCTGGTGGT 
TTCGGCCCCA CCACGGTCTC CATCTTGCAA ACACGGAAGA CAACGGTGGC CACCACCACC 
ACCACCACCG CGGCCAGCAC GGCCACCGCC ATGACACTGC AGACTAAGGG GGTCACCGAG 
TCCCTGGATC CCTGGAAAAG GACCCCTGTT GGGGTTAGCA CAACGGAGCC TTCCACTAGT 
CCCAGCAGCA ATGGGAAAGA CATCCAGCCC CCGAGGATCT TAGGGGAGAC CTCAGGTCTG 
GCTGTGCATC AGATCATCAC CATCACCGTC TCCCTCATCA TGGTCATCGC TGCGCTCATC 
ACAACTCTTG TCTTAAAAAA TTGCTGTGCC CCAAGCGGGC ACACTCGGAG GAATAGCCAC 
CAGCGGAAGA TGAATCAGCA GGAGGAGAGT TGCCAGAACT TGACGGACTT CACCCCGGCC 
CGGGTGCCCA GCAGTGTGGA CATCTTCACC GCCTATAATG AGACACTGCA GTGTTCTCAC 
GAGTGTGTCA GGGCCTCTGT GCCCGTGTAT GCCGACGAGA CGCTGCACTC GACCGGGGAG 
TACAAGTCCA CATTCAACGG AAACCGAACA TCCTCTGCGG ATAGGCATCT CATCCCTGTG 
GCCTTTGTGT CTGAGAAATG GTTTGAAATC TCCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ajap1 ( XM_006538824.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006538887.1 Mus musculus adherens junction associated protein 1 (Ajap1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu34175
Clone ID Related Accession (Same CDS sequence)	XM_006538824.4 , XM_006538824.3
Accession Version	XM_006538824.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1230bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	adherens junction-associated protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006538824.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGACAC AACTAACCAG CTCCATGTCT ATCCGCTGGC CCGGCCGCTC TCTCGGAAGC 
CATGCCTGGA TACTGATAGC CATGCTGCAG CTTGCGGTCG ACTTCCCATC CTGTGACTCA 
CTAGGCCCTG GGCCCGAGTT CCGGCTCCTG TCACGCCCAC AGAGGCCCCA GCGGCTGTGG 
AGTCTGCGAA GTGGACCCCC AACGAGGCTA CCGACACCGG CCTGGAGTCC CCGTGCTGCC 
CGGGCAGAAC GTGCCCACGG ACCCATACAG ATGCAGACTC CCCGTGCTCG GAGGGCCCAC 
AGGCCCAGGG ACCAGGTAGC CACCCTCGGA CCCAAAGGGG GACTCACCAA GCCCCCAGCT 
GCCACCAGGT CGAGCCCTTC CCTCGCCTCT GCGACGGCCT CATCCTCCAT AGTGACTGCT 
GGGGCTGCAG AGCACCAGGG CCTCCTAAGA CGGGGTAGGA GGCACACACA TGATACAGAG 
TTCAACGATT TCGACTTCCG TGGTGGCAGG CCCACGACAG AGACAGAATT CATAGCCTGG 
GGGCCTACGG GGGATGAGGA CGCCCTGGAG TCCAACACCT TCCCTGGTGG TTTCGGCCCC 
ACCACGGTCT CCATCTTGCA AACACGGAAG ACAACGGTGG CCACCACCAC CACCACCACC 
GCGGCCAGCA CGGCCACCGC CATGACACTG CAGACTAAGG GGGTCACCGA GTCCCTGGAT 
CCCTGGAAAA GGACCCCTGT TGGGGTTAGC ACAACGGAGC CTTCCACTAG TCCCAGCAGC 
AATGGGAAAG ACATCCAGCC CCCGAGGATC TTAGGGGAGA CCTCAGGTCT GGCTGTGCAT 
CAGATCATCA CCATCACCGT CTCCCTCATC ATGGTCATCG CTGCGCTCAT CACAACTCTT 
GTCTTAAAAA ATTGCTGTGC CCCAAGCGGG CACACTCGGA GGAATAGCCA CCAGCGGAAG 
ATGAATCAGC AGGAGGAGAG TTGCCAGAAC TTGACGGACT TCACCCCGGC CCGGGTGCCC 
AGCAGTGTGG ACATCTTCAC CGCCTATAAT GAGACACTGC AGTGTTCTCA CGAGTGTGTC 
AGGGCCTCTG TGCCCGTGTA TGCCGACGAG ACGCTGCACT CGACCGGGGA GTACAAGTCC 
ACATTCAACG GAAACCGAAC ATCCTCTGCG GATAGGCATC TCATCCCTGT GGCCTTTGTG 
TCTGAGAAAT GGTTTGAAAT CTCCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006538887.1
CDS	1881..3110
Translation	MQTQLTSSMSIRWPGRSLGSHAWILIAMLQLAVDFPSCDSLGPGPEFRLLSRPQRPQRLWSLRSGPPTRLPTPAWSPRAARAERAHGPIQMQTPRARRAHRPRDQVATLGPKGGLTKPPAATRSSPSLASATASSSIVTAGAAEHQGLLRRGRRHTHDTEFNDFDFRGGRPTTETEFIAWGPTGDEDALESNTFPGGFGPTTVSILQTRKTTVATTTTTTAASTATAMTLQTKGVTESLDPWKRTPVGVSTTEPSTSPSSNGKDIQPPRILGETSGLAVHQIITITVSLIMVIAALITTLVLKNCCAPSGHTRRNSHQRKMNQQEESCQNLTDFTPARVPSSVDIFTAYNETLQCSHECVRASVPVYADETLHSTGEYKSTFNGNRTSSADRHLIPVAFVSEKWFEISC

Target ORF information:

RefSeq Version	XM_006538824.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus adherens junction associated protein 1 (Ajap1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006538824.3

ORF Insert Sequence:

ATGCAGACAC AACTAACCAG CTCCATGTCT ATCCGCTGGC CCGGCCGCTC TCTCGGAAGC 
CATGCCTGGA TACTGATAGC CATGCTGCAG CTTGCGGTCG ACTTCCCATC CTGTGACTCA 
CTAGGCCCTG GGCCCGAGTT CCGGCTCCTG TCACGCCCAC AGAGGCCCCA GCGGCTGTGG 
AGTCTGCGAA GTGGACCCCC AACGAGGCTA CCGACACCGG CCTGGAGTCC CCGTGCTGCC 
CGGGCAGAAC GTGCCCACGG ACCCATACAG ATGCAGACTC CCCGTGCTCG GAGGGCCCAC 
AGGCCCAGGG ACCAGGTAGC CACCCTCGGA CCCAAAGGGG GACTCACCAA GCCCCCAGCT 
GCCACCAGGT CGAGCCCTTC CCTCGCCTCT GCGACGGCCT CATCCTCCAT AGTGACTGCT 
GGGGCTGCAG AGCACCAGGG CCTCCTAAGA CGGGGTAGGA GGCACACACA TGATACAGAG 
TTCAACGATT TCGACTTCCG TGGTGGCAGG CCCACGACAG AGACAGAATT CATAGCCTGG 
GGGCCTACGG GGGATGAGGA CGCCCTGGAG TCCAACACCT TCCCTGGTGG TTTCGGCCCC 
ACCACGGTCT CCATCTTGCA AACACGGAAG ACAACGGTGG CCACCACCAC CACCACCACC 
GCGGCCAGCA CGGCCACCGC CATGACACTG CAGACTAAGG GGGTCACCGA GTCCCTGGAT 
CCCTGGAAAA GGACCCCTGT TGGGGTTAGC ACAACGGAGC CTTCCACTAG TCCCAGCAGC 
AATGGGAAAG ACATCCAGCC CCCGAGGATC TTAGGGGAGA CCTCAGGTCT GGCTGTGCAT 
CAGATCATCA CCATCACCGT CTCCCTCATC ATGGTCATCG CTGCGCTCAT CACAACTCTT 
GTCTTAAAAA ATTGCTGTGC CCCAAGCGGG CACACTCGGA GGAATAGCCA CCAGCGGAAG 
ATGAATCAGC AGGAGGAGAG TTGCCAGAAC TTGACGGACT TCACCCCGGC CCGGGTGCCC 
AGCAGTGTGG ACATCTTCAC CGCCTATAAT GAGACACTGC AGTGTTCTCA CGAGTGTGTC 
AGGGCCTCTG TGCCCGTGTA TGCCGACGAG ACGCTGCACT CGACCGGGGA GTACAAGTCC 
ACATTCAACG GAAACCGAAC ATCCTCTGCG GATAGGCATC TCATCCCTGT GGCCTTTGTG 
TCTGAGAAAT GGTTTGAAAT CTCCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ajap1 ( XM_006538824.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006538887.1 Mus musculus adherens junction associated protein 1 (Ajap1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu34175
Clone ID Related Accession (Same CDS sequence)	XM_006538824.4 , XM_006538824.3
Accession Version	XM_006538824.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1230bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	adherens junction-associated protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006538824.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGACAC AACTAACCAG CTCCATGTCT ATCCGCTGGC CCGGCCGCTC TCTCGGAAGC 
CATGCCTGGA TACTGATAGC CATGCTGCAG CTTGCGGTCG ACTTCCCATC CTGTGACTCA 
CTAGGCCCTG GGCCCGAGTT CCGGCTCCTG TCACGCCCAC AGAGGCCCCA GCGGCTGTGG 
AGTCTGCGAA GTGGACCCCC AACGAGGCTA CCGACACCGG CCTGGAGTCC CCGTGCTGCC 
CGGGCAGAAC GTGCCCACGG ACCCATACAG ATGCAGACTC CCCGTGCTCG GAGGGCCCAC 
AGGCCCAGGG ACCAGGTAGC CACCCTCGGA CCCAAAGGGG GACTCACCAA GCCCCCAGCT 
GCCACCAGGT CGAGCCCTTC CCTCGCCTCT GCGACGGCCT CATCCTCCAT AGTGACTGCT 
GGGGCTGCAG AGCACCAGGG CCTCCTAAGA CGGGGTAGGA GGCACACACA TGATACAGAG 
TTCAACGATT TCGACTTCCG TGGTGGCAGG CCCACGACAG AGACAGAATT CATAGCCTGG 
GGGCCTACGG GGGATGAGGA CGCCCTGGAG TCCAACACCT TCCCTGGTGG TTTCGGCCCC 
ACCACGGTCT CCATCTTGCA AACACGGAAG ACAACGGTGG CCACCACCAC CACCACCACC 
GCGGCCAGCA CGGCCACCGC CATGACACTG CAGACTAAGG GGGTCACCGA GTCCCTGGAT 
CCCTGGAAAA GGACCCCTGT TGGGGTTAGC ACAACGGAGC CTTCCACTAG TCCCAGCAGC 
AATGGGAAAG ACATCCAGCC CCCGAGGATC TTAGGGGAGA CCTCAGGTCT GGCTGTGCAT 
CAGATCATCA CCATCACCGT CTCCCTCATC ATGGTCATCG CTGCGCTCAT CACAACTCTT 
GTCTTAAAAA ATTGCTGTGC CCCAAGCGGG CACACTCGGA GGAATAGCCA CCAGCGGAAG 
ATGAATCAGC AGGAGGAGAG TTGCCAGAAC TTGACGGACT TCACCCCGGC CCGGGTGCCC 
AGCAGTGTGG ACATCTTCAC CGCCTATAAT GAGACACTGC AGTGTTCTCA CGAGTGTGTC 
AGGGCCTCTG TGCCCGTGTA TGCCGACGAG ACGCTGCACT CGACCGGGGA GTACAAGTCC 
ACATTCAACG GAAACCGAAC ATCCTCTGCG GATAGGCATC TCATCCCTGT GGCCTTTGTG 
TCTGAGAAAT GGTTTGAAAT CTCCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006538887.1
CDS	98..1327
Translation	MQTQLTSSMSIRWPGRSLGSHAWILIAMLQLAVDFPSCDSLGPGPEFRLLSRPQRPQRLWSLRSGPPTRLPTPAWSPRAARAERAHGPIQMQTPRARRAHRPRDQVATLGPKGGLTKPPAATRSSPSLASATASSSIVTAGAAEHQGLLRRGRRHTHDTEFNDFDFRGGRPTTETEFIAWGPTGDEDALESNTFPGGFGPTTVSILQTRKTTVATTTTTTAASTATAMTLQTKGVTESLDPWKRTPVGVSTTEPSTSPSSNGKDIQPPRILGETSGLAVHQIITITVSLIMVIAALITTLVLKNCCAPSGHTRRNSHQRKMNQQEESCQNLTDFTPARVPSSVDIFTAYNETLQCSHECVRASVPVYADETLHSTGEYKSTFNGNRTSSADRHLIPVAFVSEKWFEISC

Target ORF information:

RefSeq Version	XM_006538824.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus adherens junction associated protein 1 (Ajap1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006538824.4

ORF Insert Sequence:

ATGCAGACAC AACTAACCAG CTCCATGTCT ATCCGCTGGC CCGGCCGCTC TCTCGGAAGC 
CATGCCTGGA TACTGATAGC CATGCTGCAG CTTGCGGTCG ACTTCCCATC CTGTGACTCA 
CTAGGCCCTG GGCCCGAGTT CCGGCTCCTG TCACGCCCAC AGAGGCCCCA GCGGCTGTGG 
AGTCTGCGAA GTGGACCCCC AACGAGGCTA CCGACACCGG CCTGGAGTCC CCGTGCTGCC 
CGGGCAGAAC GTGCCCACGG ACCCATACAG ATGCAGACTC CCCGTGCTCG GAGGGCCCAC 
AGGCCCAGGG ACCAGGTAGC CACCCTCGGA CCCAAAGGGG GACTCACCAA GCCCCCAGCT 
GCCACCAGGT CGAGCCCTTC CCTCGCCTCT GCGACGGCCT CATCCTCCAT AGTGACTGCT 
GGGGCTGCAG AGCACCAGGG CCTCCTAAGA CGGGGTAGGA GGCACACACA TGATACAGAG 
TTCAACGATT TCGACTTCCG TGGTGGCAGG CCCACGACAG AGACAGAATT CATAGCCTGG 
GGGCCTACGG GGGATGAGGA CGCCCTGGAG TCCAACACCT TCCCTGGTGG TTTCGGCCCC 
ACCACGGTCT CCATCTTGCA AACACGGAAG ACAACGGTGG CCACCACCAC CACCACCACC 
GCGGCCAGCA CGGCCACCGC CATGACACTG CAGACTAAGG GGGTCACCGA GTCCCTGGAT 
CCCTGGAAAA GGACCCCTGT TGGGGTTAGC ACAACGGAGC CTTCCACTAG TCCCAGCAGC 
AATGGGAAAG ACATCCAGCC CCCGAGGATC TTAGGGGAGA CCTCAGGTCT GGCTGTGCAT 
CAGATCATCA CCATCACCGT CTCCCTCATC ATGGTCATCG CTGCGCTCAT CACAACTCTT 
GTCTTAAAAA ATTGCTGTGC CCCAAGCGGG CACACTCGGA GGAATAGCCA CCAGCGGAAG 
ATGAATCAGC AGGAGGAGAG TTGCCAGAAC TTGACGGACT TCACCCCGGC CCGGGTGCCC 
AGCAGTGTGG ACATCTTCAC CGCCTATAAT GAGACACTGC AGTGTTCTCA CGAGTGTGTC 
AGGGCCTCTG TGCCCGTGTA TGCCGACGAG ACGCTGCACT CGACCGGGGA GTACAAGTCC 
ACATTCAACG GAAACCGAAC ATCCTCTGCG GATAGGCATC TCATCCCTGT GGCCTTTGTG 
TCTGAGAAAT GGTTTGAAAT CTCCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Tbx5 is required for avian and Mammalian epicardial formation and coronary vasculogenesis.
Circulation research115(10)834-44(2014 Oct)
Diman NY,Brooks G,Kruithof BP,Elemento O,Seidman JG,Seidman CE,Basson CT,Hatcher CJ

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

Junction protein shrew-1 influences cell invasion and interacts with invasion-promoting protein CD147.
Molecular biology of the cell18(4)1272-81(2007 Apr)
Schreiner A,Ruonala M,Jakob V,Suthaus J,Boles E,Wouters F,Starzinski-Powitz A

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Ajap1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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