Adgrb2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Adgrb2
Entrez Gene ID	230775
Full Name	adhesion G protein-coupled receptor B2
Synonyms	Bai2
General protein information	Preferred Names adhesion G protein-coupled receptor B2 Names adhesion G protein-coupled receptor B2 G protein-coupled receptor brain-specific angiogenesis inhibitor 1 brain-specific angiogenesis inhibitor 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 D2.2

mRNA and Protein(s)

mRNA	Protein	Name
XM_011240507.1	XP_011238809.1	adhesion G protein-coupled receptor B2 isoform X2
XM_011240510.1	XP_011238812.1	adhesion G protein-coupled receptor B2 isoform X6
NM_173071.3	NP_775094.2	adhesion G protein-coupled receptor B2 isoform 1 precursor
NM_001290715.1	NP_001277644.1	adhesion G protein-coupled receptor B2 isoform 3 precursor
NM_001290714.1	NP_001277643.1	adhesion G protein-coupled receptor B2 isoform 4 precursor
NM_001377057.1	NP_001363986.1	adhesion G protein-coupled receptor B2 isoform 6 precursor
XM_011240508.1	XP_011238810.1	adhesion G protein-coupled receptor B2 isoform X3
XM_011240512.1	XP_011238814.1	adhesion G protein-coupled receptor B2 isoform X5
XM_011240514.1	XP_011238816.1	adhesion G protein-coupled receptor B2 isoform X9
XM_011240506.1	XP_011238808.1	adhesion G protein-coupled receptor B2 isoform X1
XM_030253456.1	XP_030109316.1	adhesion G protein-coupled receptor B2 isoform X6
XM_030253459.1	XP_030109319.1	adhesion G protein-coupled receptor B2 isoform X9
NM_001199696.1	NP_001186625.1	adhesion G protein-coupled receptor B2 isoform 2 precursor
XM_030253457.1	XP_030109317.1	adhesion G protein-coupled receptor B2 isoform X7
XM_030253460.1	XP_030109320.1	adhesion G protein-coupled receptor B2 isoform X10
NM_001199696.2	NP_001186625.1	adhesion G protein-coupled receptor B2 isoform 2 precursor
NM_001377058.1	NP_001363987.1	adhesion G protein-coupled receptor B2 isoform 7 precursor
NM_001377056.1	NP_001363985.1	adhesion G protein-coupled receptor B2 isoform 5 precursor
NM_001290715.2	NP_001277644.1	adhesion G protein-coupled receptor B2 isoform 3 precursor
XM_011240509.1	XP_011238811.1	adhesion G protein-coupled receptor B2 isoform X4
XM_011240511.1	XP_011238813.1	adhesion G protein-coupled receptor B2 isoform X5
XM_011240513.1	XP_011238815.1	adhesion G protein-coupled receptor B2 isoform X8
XM_011240507.2	XP_011238809.1	adhesion G protein-coupled receptor B2 isoform X2
NM_173071.4	NP_775094.2	adhesion G protein-coupled receptor B2 isoform 1 precursor
NM_001290714.2	NP_001277643.1	adhesion G protein-coupled receptor B2 isoform 4 precursor

Pathways from BioSystems

Homologies

	BAI2	XP_001094702.2
Mus musculus (house mouse)	Bai2	NP_775094.2
Rattus norvegicus (Norway rat)	Bai2	NP_001101384.1
Homo sapiens (human)	BAI2	NP_001694.2
Pan troglodytes (chimpanzee)	BAI2	XP_003307988.1
Bos taurus (cattle)	BAI2	XP_003585838.2
Xenopus tropicalis (tropical clawed frog)	bai2	XP_002938534.2
Canis lupus familiaris (dog)	BAI2	XP_005617717.1
Gallus gallus (chicken)	BAI2	XP_423828.4

Gene Ontology
Bibliography

Related articles in PubMed

Whole exome sequencing reveals a functional mutation in the GAIN domain of the Bai2 receptor underlying a forward mutagenesis hyperactivity QTL.
Speca DJ, Trimmer JS, Peterson AS, D?az E
Mammalian genome : official journal of the International Mammalian Genome Society28(11-12)465-475(2017 Dec)

International Union of Basic and Clinical Pharmacology. XCIV. Adhesion G protein-coupled receptors.
Hamann J, Aust G, Ara? D, Engel FB, Formstone C, Fredriksson R, Hall RA, Harty BL, Kirchhoff C, Knapp B, Krishnan A, Liebscher I, Lin HH, Martinelli DC, Monk KR, Peeters MC, Piao X, Pr?mel S, Sch?neberg T, Schwartz TW, Singer K, Stacey M, Ushkaryov YA, Vallon M, Wolfrum U, Wright MW, Xu L, Langenhan T, Schi?th HB
Pharmacological reviews67(2)338-67(2015)

Anterograde C1ql1 signaling is required in order to determine and maintain a single-winner climbing fiber in the mouse cerebellum.
Kakegawa W, Mitakidis N, Miura E, Abe M, Matsuda K, Takeo YH, Kohda K, Motohashi J, Takahashi A, Nagao S, Muramatsu S, Watanabe M, Sakimura K, Aricescu AR, Yuzaki M
Neuron85(2)316-29(2015 Jan)

Calsyntenins function as synaptogenic adhesion molecules in concert with neurexins.
Um JW, Pramanik G, Ko JS, Song MY, Lee D, Kim H, Park KS, S?dhof TC, Tabuchi K, Ko J
Cell reports6(6)1096-1109(2014 Mar)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

BAI2 is a functional GPCR regulated by proteolytic processing and activates the NFAT pathway
Title: Brain-specific angiogenesis inhibitor 2 (BAI2) may be activated by proteolytic processing.

Results point out that brain-specific angiogenesis inhibitor 2 controls VEGF transcription through GA-binding protein under normal conditions and cerebral ischemia.
Title: Brain-specific angiogenesis inhibitor 2 regulates VEGF through GABP that acts as a transcriptional repressor.

The following Adgrb2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Adgrb2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu16824	NM_001290714.2 Latest version!	Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu73520	NM_001377057.1 Latest version!	Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu90667	NM_001377058.1 Latest version!	Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu73521	XM_030253459.1 Latest version!	Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu88509	XM_030253460.1 Latest version!	Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu14259	NM_001199696.2 Latest version!	Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu14294	NM_001290715.2 Latest version!	Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu14311	NM_173071.4 Latest version!	Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu73516	NM_001377056.1 Latest version!	Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu73514	XM_011240507.2 Latest version!	Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu73517	XM_030253457.1 Latest version!	Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu73518	XM_030253456.1 Latest version!	Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu73513	XM_011240506.1 Latest version!	Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu73515	XM_011240508.1 Latest version!	Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu73516	XM_011240509.1 Latest version!	Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu73517	XM_011240510.1 Latest version!	Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu73518	XM_011240511.1 Latest version!	Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu73519	XM_011240512.1 Latest version!	Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu73520	XM_011240513.1 Latest version!	Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu73521	XM_011240514.1 Latest version!	Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
View Old Accession Versions >>
OMu14259	NM_001199696.1	Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu14294	NM_001290715.1	Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu73514	XM_011240507.1	Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu14311	NM_173071.3	Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$909.30 $1299.00
OMu16824	NM_001290714.1	Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Adgrb2 ( NM_001290714.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001277643.1 Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OMu16824
Clone ID Related Accession (Same CDS sequence)	NM_001290714.1 , NM_001290714.2
Accession Version	NM_001290714.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-11
Organism	Mus musculus(house mouse)
Product	adhesion G protein-coupled receptor B2 isoform 4 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL626774.23. On Dec 12, 2019 this sequence version replaced NM_001290714.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK147485.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849379 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCCCAG CCTGTCCCCT CTTACTGTCT GTGATTCTGT CCCTGCGCCT GGCCACGGCC 
TTCGACCCTG CCCCCAGTGC CTGCTCTGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTC 
TCGCTGCAGG ACCTCTTTCC CACCATCGCC TCGGGCTGCT CCTGGACCCT GGAGAACCCA 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGGC AGGAGCAGGT TTGCACACAC 
TTTGCCCCGC GCCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTTACCTG CCTGCGGCCT 
GGTCCAGAGG AAGCCACAGC CCGGGCTGAG TCGGAGGTGG GACGGCCAGA GGAGGAGGAG 
GAGGAGGCGG CGGCAGCAGC ATCAGGGTTG GAGTTGTGTG GTGGCTCAGG CCCCTTTACC 
TTTCTGCACT TCGACAAGAA CTTTGTGCAG CTGTGCCTGT CGGCTGAGCC CTCTGAGGCC 
CCTCGTCTGC TAGCGCCTGC TGCCCTGGCC TTCCGTTTTG TGGAGGTCTT GCTGATCAAC 
AACAACAACT CCAGCCAGTT CACCTGTGGT GTGCTCTGCC GCTGGAGTGA GGAGTGTGGC 
CGGGCTGCAG GCAGGGCTTG TGGCTTTGCA CAGCCAGGGT GTAGTTGTCC TGGGGAGGCA 
GGGGCCAACC CCGCCACCAC CACATCTCCG GGGCCTCCGG TTGCCCACAC CCTGTCCAAT 
GCCCTGGTGC CCGGGGGCCC AGCCCCTCCT GCTGAGGCCG ACTTGCACTC GGGGAGCAGC 
AATGACCTGT TCACCACCGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG 
AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTAT ACATGGCGCA GACAGGCGAC 
CCAGCAGCTG AGGAGTGGTC CCCGTGGAGC GTGTGTTCCC TGACGTGTGG GCAGGGTCTG 
CAGGTGCGGA CCCGCTCCTG CGTGTCCTCC CCCTATGGGA CCCTGTGCAG CGGGCCCCTT 
CGGGAGACCC GGCCTTGCAA CAATTCAGCC ACCTGCCCAG TGGAAGGCCA GTGGCTAGAA 
TGGGGTCCCT GGGGCCCATG CTCATCATCT TGTGCCAATG GGACCCAGCA GCGCAGCCGG 
AAATGCAGTG TGGCGGGTCC AGCCTGGGCC ACGTGCGCAG GTGCCCTCAC GGATACCCGT 
GAGTGCAGCA ATCTCGATTG CCCGGCCACT GACGGCAAGT GGGGGCCGTG GAACGCGTGG 
AGCCTGTGCT CCAAGACGTG TGACACGGGC TGGCAACGCC GCTTCCGCAT GTGCCAGGCT 
TCTGGCACAC AGGGCTACCC TTGCGAGGGC ACAGGAGAGG AGGTGAAACC CTGCAGTGAG 
AAGAGGTGTC CAGCCTTCCA TGAGATGTGC AGGGATGAGT ACGTGATGTT GATGACATGG 
AAGAGGGCGG CAGCTGGCGA GATCATTTAC AACAAGTGTC CCCCTAATGC CTCGGGTTCT 
GCTAGCCGCC GCTGTCTCCT CAGTGCCCAG GGCGTAGCAT ACTGGGGACT GCCCAGCTTT 
GCTCGTTGCA TATCCCATGA ATACCGCTAC CTGTACCTGT CACTTCGGGA ACACCTGGCT 
AAGGGCCAGC GCATGCTGGC AGGTGAGGGC ATGTCACAGG TGGTGCGGAG CCTGCAGGAG 
CTACTGGCAC GGCGCACTTA CTACAGCGGG GACCTGCTCT TCTCTGTGGA CATCCTAAGG 
AACGTCACTG ACACCTTCAA GAGGGCCACC TATGTCCCTT CCGCCGATGA CGTGCAGCGT 
TTCTTCCAGG TGGTGAGCTT CATGGTGGAT TCAGAAAACA AGGACAAATG GGATGATGCT 
CAGCAGGTGT CACCGGGCTC TGTGCACCTG CTGCGTGTTG TGGAAGATTT CATTCACCTC 
GTGGGCGACG CTCTCAAGGC CTTCCAGAGC TCTCTCATTG TCACGGACAA TCTGGTGATC 
AGCATTCAGA GAGAGCCTAT CTCCGCCGTG TCCAGTGACA TCACGTTTCC CATGCGGGGC 
CGCAGGGGCA TGAAGGACTG GGTGCGACAC TCAGAGGATC GTCTCTTTCT ACCCAAGGAG 
GTGCTCAGCC TGTCCTCCCC AGGAAAGCCA GCCACACCTG GGGCAGCCAC AGCAGGCAGC 
CCGGGCAGGG GGAGGGGCCC AGGAACGGTG CCCCCTGGCC CAGGCCACGC CCACCAGCGC 
CTTCTCCCAG CTGACCCCGA AGAGTCCTCC TCCTACTTTG TGATCGGTGC TGTGCTCTAC 
CGCACCCTTG GCCTCATCCT GCCGCCCCCC AGGCCTCCAC TTGCTGTCAC CTCCCGGGTG 
ATGACAGTGA CTGTGCGTCC CCCCACCCAG CCTCCAGCTG AGCCCCTCAT TACAGTGGAA 
CTCTCGTACA TCATCAATGG CACCACCGAT CCCCACTGTG CCAGCTGGGA CTACTCCAGA 
GCAGATACCA ACTCGGGGGA CTGGAACACT GAGAGCTGCC AGACCTTGGA GACCCAGGCG 
GCTCACACCC GCTGCCAGTG CCAGCACCTG TCCACCTTTG CCGTCCTGGC CCAGCCACCC 
AAGGACCTGA CCCTGGAGCT GGCAGGTGCT CCCTCTGTCC CCCTGGTGAT CGGCTGTGCA 
GTGTCCTGCA TGGCTCTGCT CACCCTGCTG GCCATCTATG CAGCCTTCTG GAGGTTTATA 
AAATCAGAAC GCTCCATCAT CTTGCTGAAC TTCTGCCTGT CCATCCTGGC TTCCAACATT 
CTGATCCTGG TGGGCCAGTC CCGGGTGCTG AGCAAGGGCG TATGCACCAT GACGGCTGCC 
TTCCTACACT TCTTCTTTCT GTCCTCCTTT TGCTGGGTGC TTACAGAGGC CTGGCAATCC 
TATCTGGCTG TCATCGGGCG GATGCGCACC CGCCTGGTTC GCAAGCGCTT CCTCTGCCTG 
GGCTGGGGTC TACCTGCCCT GGTGGTGGCT GTGTCTGTTG GCTTTACTCG CACCAAAGGA 
TATGGTACAT CCAGCTACTG CTGGCTGTCC CTAGAGGGCG GCCTGCTCTA TGCCTTCGTG 
GGTCCAGCAG CAGTCATTGT CCTGGTGAAC ATGCTCATCG GGATTATCGT CTTCAACAAG 
CTCATGGCTC GCGATGGCGT CTCAGACAAA TCTAAGAAGC AGAGGGCTGG GGCTTCACTC 
TGGAGTTCCT GCGTGGTACT GCCTCTCCTG GCGCTTACCT GGATGTCTGC CGTCCTGGCC 
ATGACAGATC GCCGCTCCGT CCTCTTCCAG GCACTCTTTG CCGTTTTCAA CTCTGCACAA 
GGCTTTGTCA TCACCGCTGT GCACTGCTTC CTGCGCCGAG AGGTCCAGGA TGTGGTAAAG 
TGTCAGATGG GTGTGTGTCG GGCTGATGAG AGTGAAGACT CCCCAGACTC GTGCAAGAAC 
GGGCAGCTGC AGATCCTGTC AGACTTTGAA AAGGACGTGG ATCTGGCTTG TCAGACAGTT 
CTGTTCAAGG AGGTCAACAC CTGCAACCCG TCCACCATTA CCGGCACTCT GTCCCGCCTG 
TCTCTGGATG AGGATGAGGA GCCCAAGTCC TGTCTCGTGG GTCCTGAGGG TGGCCTCAGC 
TTCTCACCAC TGCCTGGGAA CATCCTGGTA CCCATGGCAG CCTCACCAGG TCTAGGGGAG 
CCACCACCAC CCCAGGAGAC CAACCCTGTG TACATGTGTG GGGAGGGTGG CCTGCGGCAG 
TTGGACCTTA CATGGATACG GCAGAGTGAA CCAGGCTCTG AGGGGGACTA CATGGTTCTG 
CCCCGGCGGA CTTTGAGCCT GCAGCCTGGT GGTGGGGGTA CAGCGGGTGA GGAGGCCCCA 
AGGGCCCGGC CTGAGGGGAC CCCCCGGCGG GCTGCCAAAA CGGTAGCCCA CACTGAAGGC 
TACCCCAGCT TCCTGTCTGT GGAGCACTCG GGTCTAGGGC TGGGCCCTGC CTATGGGTCT 
CTCCAGAACC CGTATGGAAT GACCTTCCAA CCACCACCAC CAACACCCAG CGCCCGCCAA 
GTACCAGAGC CAGGAGAACG TAGCCGGACC ATGCCCCGTA CAGTGCCTGG TTCCACCATG 
AAGCTGGGCT CCCTTGAGCG AAAGAAGCTT CGGTATTCGG ACTTGGACTT TGAGAAGGTG 
ATGCACACTC GGAAACGGCA CTCGGAACTC TACCACGAAC TCAACCAGAA GTTCCACACT 
TTCGACCGCT ACCGTAGCCA GTCCTCAGCC AAGGAGAAAC CCAGCCCCCC CGGGGGACGC 
CCTGGCTTGT CCCAGCACAG GAGGCATCAA AGCTGGAGCA CCTTCAAATC TATGACACTG 
GGCTCACTGC CCCCCAAGCC CCGAGAACGG CTGGCCCTGC ACCGGACAGC AGCCTGGGAG 
CCCACAGAAC CGCCAGACGG CGACTTCCAG ACAGAGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001277643.1
CDS	454..4875
Translation	MTPACPLLLSVILSLRLATAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCTHFAPRLLPLDHYLVNFTCLRPGPEEATARAESEVGRPEEEEEEAAAAASGLELCGGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGANPATTTSPGPPVAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVEGQWLEWGPWGPCSSSCANGTQQRSRKCSVAGPAWATCAGALTDTRECSNLDCPATDGKWGPWNAWSLCSKTCDTGWQRRFRMCQASGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKRAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDSENKDKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPISAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSSPGKPATPGAATAGSPGRGRGPGTVPPGPGHAHQRLLPADPEESSSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADTNSGDWNTESCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGAPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNILILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGVSDKSKKQRAGASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGGLSFSPLPGNILVPMAASPGLGEPPPPQETNPVYMCGEGGLRQLDLTWIRQSEPGSEGDYMVLPRRTLSLQPGGGGTAGEEAPRARPEGTPRRAAKTVAHTEGYPSFLSVEHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVPEPGERSRTMPRTVPGSTMKLGSLERKKLRYSDLDFEKVMHTRKRHSELYHELNQKFHTFDRYRSQSSAKEKPSPPGGRPGLSQHRRHQSWSTFKSMTLGSLPPKPRERLALHRTAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	NM_001290714.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001290714.2

ORF Insert Sequence:

ATGACCCCAG CCTGTCCCCT CTTACTGTCT GTGATTCTGT CCCTGCGCCT GGCCACGGCC 
TTCGACCCTG CCCCCAGTGC CTGCTCTGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTC 
TCGCTGCAGG ACCTCTTTCC CACCATCGCC TCGGGCTGCT CCTGGACCCT GGAGAACCCA 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGGC AGGAGCAGGT TTGCACACAC 
TTTGCCCCGC GCCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTTACCTG CCTGCGGCCT 
GGTCCAGAGG AAGCCACAGC CCGGGCTGAG TCGGAGGTGG GACGGCCAGA GGAGGAGGAG 
GAGGAGGCGG CGGCAGCAGC ATCAGGGTTG GAGTTGTGTG GTGGCTCAGG CCCCTTTACC 
TTTCTGCACT TCGACAAGAA CTTTGTGCAG CTGTGCCTGT CGGCTGAGCC CTCTGAGGCC 
CCTCGTCTGC TAGCGCCTGC TGCCCTGGCC TTCCGTTTTG TGGAGGTCTT GCTGATCAAC 
AACAACAACT CCAGCCAGTT CACCTGTGGT GTGCTCTGCC GCTGGAGTGA GGAGTGTGGC 
CGGGCTGCAG GCAGGGCTTG TGGCTTTGCA CAGCCAGGGT GTAGTTGTCC TGGGGAGGCA 
GGGGCCAACC CCGCCACCAC CACATCTCCG GGGCCTCCGG TTGCCCACAC CCTGTCCAAT 
GCCCTGGTGC CCGGGGGCCC AGCCCCTCCT GCTGAGGCCG ACTTGCACTC GGGGAGCAGC 
AATGACCTGT TCACCACCGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG 
AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTAT ACATGGCGCA GACAGGCGAC 
CCAGCAGCTG AGGAGTGGTC CCCGTGGAGC GTGTGTTCCC TGACGTGTGG GCAGGGTCTG 
CAGGTGCGGA CCCGCTCCTG CGTGTCCTCC CCCTATGGGA CCCTGTGCAG CGGGCCCCTT 
CGGGAGACCC GGCCTTGCAA CAATTCAGCC ACCTGCCCAG TGGAAGGCCA GTGGCTAGAA 
TGGGGTCCCT GGGGCCCATG CTCATCATCT TGTGCCAATG GGACCCAGCA GCGCAGCCGG 
AAATGCAGTG TGGCGGGTCC AGCCTGGGCC ACGTGCGCAG GTGCCCTCAC GGATACCCGT 
GAGTGCAGCA ATCTCGATTG CCCGGCCACT GACGGCAAGT GGGGGCCGTG GAACGCGTGG 
AGCCTGTGCT CCAAGACGTG TGACACGGGC TGGCAACGCC GCTTCCGCAT GTGCCAGGCT 
TCTGGCACAC AGGGCTACCC TTGCGAGGGC ACAGGAGAGG AGGTGAAACC CTGCAGTGAG 
AAGAGGTGTC CAGCCTTCCA TGAGATGTGC AGGGATGAGT ACGTGATGTT GATGACATGG 
AAGAGGGCGG CAGCTGGCGA GATCATTTAC AACAAGTGTC CCCCTAATGC CTCGGGTTCT 
GCTAGCCGCC GCTGTCTCCT CAGTGCCCAG GGCGTAGCAT ACTGGGGACT GCCCAGCTTT 
GCTCGTTGCA TATCCCATGA ATACCGCTAC CTGTACCTGT CACTTCGGGA ACACCTGGCT 
AAGGGCCAGC GCATGCTGGC AGGTGAGGGC ATGTCACAGG TGGTGCGGAG CCTGCAGGAG 
CTACTGGCAC GGCGCACTTA CTACAGCGGG GACCTGCTCT TCTCTGTGGA CATCCTAAGG 
AACGTCACTG ACACCTTCAA GAGGGCCACC TATGTCCCTT CCGCCGATGA CGTGCAGCGT 
TTCTTCCAGG TGGTGAGCTT CATGGTGGAT TCAGAAAACA AGGACAAATG GGATGATGCT 
CAGCAGGTGT CACCGGGCTC TGTGCACCTG CTGCGTGTTG TGGAAGATTT CATTCACCTC 
GTGGGCGACG CTCTCAAGGC CTTCCAGAGC TCTCTCATTG TCACGGACAA TCTGGTGATC 
AGCATTCAGA GAGAGCCTAT CTCCGCCGTG TCCAGTGACA TCACGTTTCC CATGCGGGGC 
CGCAGGGGCA TGAAGGACTG GGTGCGACAC TCAGAGGATC GTCTCTTTCT ACCCAAGGAG 
GTGCTCAGCC TGTCCTCCCC AGGAAAGCCA GCCACACCTG GGGCAGCCAC AGCAGGCAGC 
CCGGGCAGGG GGAGGGGCCC AGGAACGGTG CCCCCTGGCC CAGGCCACGC CCACCAGCGC 
CTTCTCCCAG CTGACCCCGA AGAGTCCTCC TCCTACTTTG TGATCGGTGC TGTGCTCTAC 
CGCACCCTTG GCCTCATCCT GCCGCCCCCC AGGCCTCCAC TTGCTGTCAC CTCCCGGGTG 
ATGACAGTGA CTGTGCGTCC CCCCACCCAG CCTCCAGCTG AGCCCCTCAT TACAGTGGAA 
CTCTCGTACA TCATCAATGG CACCACCGAT CCCCACTGTG CCAGCTGGGA CTACTCCAGA 
GCAGATACCA ACTCGGGGGA CTGGAACACT GAGAGCTGCC AGACCTTGGA GACCCAGGCG 
GCTCACACCC GCTGCCAGTG CCAGCACCTG TCCACCTTTG CCGTCCTGGC CCAGCCACCC 
AAGGACCTGA CCCTGGAGCT GGCAGGTGCT CCCTCTGTCC CCCTGGTGAT CGGCTGTGCA 
GTGTCCTGCA TGGCTCTGCT CACCCTGCTG GCCATCTATG CAGCCTTCTG GAGGTTTATA 
AAATCAGAAC GCTCCATCAT CTTGCTGAAC TTCTGCCTGT CCATCCTGGC TTCCAACATT 
CTGATCCTGG TGGGCCAGTC CCGGGTGCTG AGCAAGGGCG TATGCACCAT GACGGCTGCC 
TTCCTACACT TCTTCTTTCT GTCCTCCTTT TGCTGGGTGC TTACAGAGGC CTGGCAATCC 
TATCTGGCTG TCATCGGGCG GATGCGCACC CGCCTGGTTC GCAAGCGCTT CCTCTGCCTG 
GGCTGGGGTC TACCTGCCCT GGTGGTGGCT GTGTCTGTTG GCTTTACTCG CACCAAAGGA 
TATGGTACAT CCAGCTACTG CTGGCTGTCC CTAGAGGGCG GCCTGCTCTA TGCCTTCGTG 
GGTCCAGCAG CAGTCATTGT CCTGGTGAAC ATGCTCATCG GGATTATCGT CTTCAACAAG 
CTCATGGCTC GCGATGGCGT CTCAGACAAA TCTAAGAAGC AGAGGGCTGG GGCTTCACTC 
TGGAGTTCCT GCGTGGTACT GCCTCTCCTG GCGCTTACCT GGATGTCTGC CGTCCTGGCC 
ATGACAGATC GCCGCTCCGT CCTCTTCCAG GCACTCTTTG CCGTTTTCAA CTCTGCACAA 
GGCTTTGTCA TCACCGCTGT GCACTGCTTC CTGCGCCGAG AGGTCCAGGA TGTGGTAAAG 
TGTCAGATGG GTGTGTGTCG GGCTGATGAG AGTGAAGACT CCCCAGACTC GTGCAAGAAC 
GGGCAGCTGC AGATCCTGTC AGACTTTGAA AAGGACGTGG ATCTGGCTTG TCAGACAGTT 
CTGTTCAAGG AGGTCAACAC CTGCAACCCG TCCACCATTA CCGGCACTCT GTCCCGCCTG 
TCTCTGGATG AGGATGAGGA GCCCAAGTCC TGTCTCGTGG GTCCTGAGGG TGGCCTCAGC 
TTCTCACCAC TGCCTGGGAA CATCCTGGTA CCCATGGCAG CCTCACCAGG TCTAGGGGAG 
CCACCACCAC CCCAGGAGAC CAACCCTGTG TACATGTGTG GGGAGGGTGG CCTGCGGCAG 
TTGGACCTTA CATGGATACG GCAGAGTGAA CCAGGCTCTG AGGGGGACTA CATGGTTCTG 
CCCCGGCGGA CTTTGAGCCT GCAGCCTGGT GGTGGGGGTA CAGCGGGTGA GGAGGCCCCA 
AGGGCCCGGC CTGAGGGGAC CCCCCGGCGG GCTGCCAAAA CGGTAGCCCA CACTGAAGGC 
TACCCCAGCT TCCTGTCTGT GGAGCACTCG GGTCTAGGGC TGGGCCCTGC CTATGGGTCT 
CTCCAGAACC CGTATGGAAT GACCTTCCAA CCACCACCAC CAACACCCAG CGCCCGCCAA 
GTACCAGAGC CAGGAGAACG TAGCCGGACC ATGCCCCGTA CAGTGCCTGG TTCCACCATG 
AAGCTGGGCT CCCTTGAGCG AAAGAAGCTT CGGTATTCGG ACTTGGACTT TGAGAAGGTG 
ATGCACACTC GGAAACGGCA CTCGGAACTC TACCACGAAC TCAACCAGAA GTTCCACACT 
TTCGACCGCT ACCGTAGCCA GTCCTCAGCC AAGGAGAAAC CCAGCCCCCC CGGGGGACGC 
CCTGGCTTGT CCCAGCACAG GAGGCATCAA AGCTGGAGCA CCTTCAAATC TATGACACTG 
GGCTCACTGC CCCCCAAGCC CCGAGAACGG CTGGCCCTGC ACCGGACAGC AGCCTGGGAG 
CCCACAGAAC CGCCAGACGG CGACTTCCAG ACAGAGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adgrb2 ( NM_001377057.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001363986.1 Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant 6, mRNA.

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CloneID	OMu73520
Clone ID Related Accession (Same CDS sequence)	NM_001377057.1 , XM_011240513.1
Accession Version	NM_001377057.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-11
Organism	Mus musculus(house mouse)
Product	adhesion G protein-coupled receptor B2 isoform 6 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL626774.23. On Dec 12, 2019 this sequence version replaced XM_030253458.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7652917.182517.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849379 [ECO:0000348] ##Evidence-Data-END##

ATGACCCCAG CCTGTCCCCT CTTACTGTCT GTGATTCTGT CCCTGCGCCT GGCCACGGCC 
TTCGACCCTG CCCCCAGTGC CTGCTCTGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTC 
TCGCTGCAGG ACCTCTTTCC CACCATCGCC TCGGGCTGCT CCTGGACCCT GGAGAACCCA 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGGC AGGAGCAGGT TTGCACACAC 
TTTGCCCCGC GCCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTTACCTG CCTGCGGCCT 
GGTCCAGAGG AAGCCACAGC CCGGGCTGAG TCGGAGGTGG GACGGCCAGA GGAGGAGGAG 
GAGGAGGCGG CGGCAGCAGC ATCAGGGTTG GAGTTGTGTG GTGGCTCAGG CCCCTTTACC 
TTTCTGCACT TCGACAAGAA CTTTGTGCAG CTGTGCCTGT CGGCTGAGCC CTCTGAGGCC 
CCTCGTCTGC TAGCGCCTGC TGCCCTGGCC TTCCGTTTTG TGGAGGTCTT GCTGATCAAC 
AACAACAACT CCAGCCAGTT CACCTGTGGT GTGCTCTGCC GCTGGAGTGA GGAGTGTGGC 
CGGGCTGCAG GCAGGGCTTG TGGCTTTGCA CAGCCAGGGT GTAGTTGTCC TGGGGAGGCA 
GGGGCCAACC CCGCCACCAC CACATCTCCG GGGCCTCCGG TTGCCCACAC CCTGTCCAAT 
GCCCTGGTGC CCGGGGGCCC AGCCCCTCCT GCTGAGGCCG ACTTGCACTC GGGGAGCAGC 
AATGACCTGT TCACCACCGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG 
AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTAT ACATGGCGCA GACAGTGCAC 
GGCGTGTGGG AGGAGTGGGG GTCCTGGAGC CTGTGCTCCC GCAGCTGCGG GCGGGGGTCT 
CGGAGCCGGA TGCGGACCTG CGTGCCCCCC CAGCACGGCG GCAAGGCCTG CGAGGGTCCC 
GAGCTGCAGA CTAAACTCTG CAGTATGGCC GCCTGCCCGG TGGAAGGCCA GTGGCTAGAA 
TGGGGTCCCT GGGGCCCATG CTCATCATCT TGTGCCAATG GGACCCAGCA GCGCAGCCGG 
AAATGCAGTG TGGCGGGTCC AGCCTGGGCC ACGTGCGCAG GTGCCCTCAC GGATACCCGT 
GAGTGCAGCA ATCTCGATTG CCCGGCCACT GACGGCAAGT GGGGGCCGTG GAACGCGTGG 
AGCCTGTGCT CCAAGACGTG TGACACGGGC TGGCAACGCC GCTTCCGCAT GTGCCAGGCT 
TCTGGCACAC AGGGCTACCC TTGCGAGGGC ACAGGAGAGG AGGTGAAACC CTGCAGTGAG 
AAGAGGTGTC CAGCCTTCCA TGAGATGTGC AGGGATGAGT ACGTGATGTT GATGACATGG 
AAGAGGGCGG CAGCTGGCGA GATCATTTAC AACAAGTGTC CCCCTAATGC CTCGGGTTCT 
GCTAGCCGCC GCTGTCTCCT CAGTGCCCAG GGCGTAGCAT ACTGGGGACT GCCCAGCTTT 
GCTCGTTGCA TATCCCATGA ATACCGCTAC CTGTACCTGT CACTTCGGGA ACACCTGGCT 
AAGGGCCAGC GCATGCTGGC AGGTGAGGGC ATGTCACAGG TGGTGCGGAG CCTGCAGGAG 
CTACTGGCAC GGCGCACTTA CTACAGCGGG GACCTGCTCT TCTCTGTGGA CATCCTAAGG 
AACGTCACTG ACACCTTCAA GAGGGCCACC TATGTCCCTT CCGCCGATGA CGTGCAGCGT 
TTCTTCCAGG TGGTGAGCTT CATGGTGGAT TCAGAAAACA AGGACAAATG GGATGATGCT 
CAGCAGGTGT CACCGGGCTC TGTGCACCTG CTGCGTGTTG TGGAAGATTT CATTCACCTC 
GTGGGCGACG CTCTCAAGGC CTTCCAGAGC TCTCTCATTG TCACGGACAA TCTGGTGATC 
AGCATTCAGA GAGAGCCTAT CTCCGCCGTG TCCAGTGACA TCACGTTTCC CATGCGGGGC 
CGCAGGGGCA TGAAGGACTG GGTGCGACAC TCAGAGGATC GTCTCTTTCT ACCCAAGGAG 
GTGCTCAGCC TGTCCTCCCC AGGAAAGCCA GCCACACCTG GGGCAGCCAC AGCAGGCAGC 
CCGGGCAGGG GGAGGGGCCC AGGAACGGTG CCCCCTGGCC CAGGCCACGC CCACCAGCGC 
CTTCTCCCAG CTGACCCCGA AGAGTCCTCC TCCTACTTTG TGATCGGTGC TGTGCTCTAC 
CGCACCCTTG GCCTCATCCT GCCGCCCCCC AGGCCTCCAC TTGCTGTCAC CTCCCGGGTG 
ATGACAGTGA CTGTGCGTCC CCCCACCCAG CCTCCAGCTG AGCCCCTCAT TACAGTGGAA 
CTCTCGTACA TCATCAATGG CACCACCGAT CCCCACTGTG CCAGCTGGGA CTACTCCAGA 
GCAGATACCA ACTCGGGGGA CTGGAACACT GAGAGCTGCC AGACCTTGGA GACCCAGGCG 
GCTCACACCC GCTGCCAGTG CCAGCACCTG TCCACCTTTG CCGTCCTGGC CCAGCCACCC 
AAGGACCTGA CCCTGGAGCT GGCAGGTGCT CCCTCTGTCC CCCTGGTGAT CGGCTGTGCA 
GTGTCCTGCA TGGCTCTGCT CACCCTGCTG GCCATCTATG CAGCCTTCTG GAGGTTTATA 
AAATCAGAAC GCTCCATCAT CTTGCTGAAC TTCTGCCTGT CCATCCTGGC TTCCAACATT 
CTGATCCTGG TGGGCCAGTC CCGGGTGCTG AGCAAGGGCG TATGCACCAT GACGGCTGCC 
TTCCTACACT TCTTCTTTCT GTCCTCCTTT TGCTGGGTGC TTACAGAGGC CTGGCAATCC 
TATCTGGCTG TCATCGGGCG GATGCGCACC CGCCTGGTTC GCAAGCGCTT CCTCTGCCTG 
GGCTGGGGTC TACCTGCCCT GGTGGTGGCT GTGTCTGTTG GCTTTACTCG CACCAAAGGA 
TATGGTACAT CCAGCTACTG CTGGCTGTCC CTAGAGGGCG GCCTGCTCTA TGCCTTCGTG 
GGTCCAGCAG CAGTCATTGT CCTGGTGAAC ATGCTCATCG GGATTATCGT CTTCAACAAG 
CTCATGGCTC GCGATGGCGT CTCAGACAAA TCTAAGAAGC AGAGGGCTGG GGCTTCACTC 
TGGAGTTCCT GCGTGGTACT GCCTCTCCTG GCGCTTACCT GGATGTCTGC CGTCCTGGCC 
ATGACAGATC GCCGCTCCGT CCTCTTCCAG GCACTCTTTG CCGTTTTCAA CTCTGCACAA 
GGCTTTGTCA TCACCGCTGT GCACTGCTTC CTGCGCCGAG AGGTCCAGGA TGTGGTAAAG 
TGTCAGATGG GTGTGTGTCG GGCTGATGAG AGTGAAGACT CCCCAGACTC GTGCAAGAAC 
GGGCAGCTGC AGATCCTGTC AGACTTTGAA AAGGACGTGG ATCTGGCTTG TCAGACAGTT 
CTGTTCAAGG AGGTCAACAC CTGCAACCCG TCCACCATTA CCGGCACTCT GTCCCGCCTG 
TCTCTGGATG AGGATGAGGA GCCCAAGTCC TGTCTCGTGG GTCCTGAGGG TGGCCTCAGC 
TTCTCACCAC TGCCTGGGAA CATCCTGGTA CCCATGGCAG CCTCACCAGG TCTAGGGGAG 
CCACCACCAC CCCAGGAGAC CAACCCTGTG TACATGTGTG GGGAGGGTGG CCTGCGGCAG 
TTGGACCTTA CATGGATACG GCAGAGTGAA CCAGGCTCTG AGGGGGACTA CATGGTTCTG 
CCCCGGCGGA CTTTGAGCCT GCAGCCTGGT GGTGGGGGTA CAGCGGGTGA GGAGGCCCCA 
AGGGCCCGGC CTGAGGGGAC CCCCCGGCGG GCTGCCAAAA CGGTAGCCCA CACTGAAGGC 
TACCCCAGCT TCCTGTCTGT GGAGCACTCG GGTCTAGGGC TGGGCCCTGC CTATGGGTCT 
CTCCAGAACC CGTATGGAAT GACCTTCCAA CCACCACCAC CAACACCCAG CGCCCGCCAA 
GTACCAGAGC CAGGAGAACG TAGCCGGACC ATGCCCCGTA CAGTGCCTGG TTCCACCATG 
AAGCTGGGCT CCCTTGAGCG AAAGAAGCTT CGGTATTCGG ACTTGGACTT TGAGAAGGTG 
ATGCACACTC GGAAACGGCA CTCGGAACTC TACCACGAAC TCAACCAGAA GTTCCACACT 
TTCGACCGCT ACCGTAGCCA GTCCTCAGCC AAGGAGAAAC CCAGCCCCCC CGGGGGACGC 
CCTGGCTTGT CCCAGCACAG GAGGCATCAA AGCTGGAGCA CCTTCAAATC TATGACACTG 
GGCTCACTGC CCCCCAAGCC CCGAGAACGG CTGGCCCTGC ACCGGACAGC AGCCTGGGAG 
CCCACAGAAC CGCCAGACGG CGACTTCCAG ACAGAGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363986.1
CDS	454..4875
Translation	MTPACPLLLSVILSLRLATAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCTHFAPRLLPLDHYLVNFTCLRPGPEEATARAESEVGRPEEEEEEAAAAASGLELCGGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGANPATTTSPGPPVAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSSSCANGTQQRSRKCSVAGPAWATCAGALTDTRECSNLDCPATDGKWGPWNAWSLCSKTCDTGWQRRFRMCQASGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKRAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDSENKDKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPISAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSSPGKPATPGAATAGSPGRGRGPGTVPPGPGHAHQRLLPADPEESSSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADTNSGDWNTESCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGAPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNILILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGVSDKSKKQRAGASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGGLSFSPLPGNILVPMAASPGLGEPPPPQETNPVYMCGEGGLRQLDLTWIRQSEPGSEGDYMVLPRRTLSLQPGGGGTAGEEAPRARPEGTPRRAAKTVAHTEGYPSFLSVEHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVPEPGERSRTMPRTVPGSTMKLGSLERKKLRYSDLDFEKVMHTRKRHSELYHELNQKFHTFDRYRSQSSAKEKPSPPGGRPGLSQHRRHQSWSTFKSMTLGSLPPKPRERLALHRTAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	NM_001377057.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001377057.1

ORF Insert Sequence:

ATGACCCCAG CCTGTCCCCT CTTACTGTCT GTGATTCTGT CCCTGCGCCT GGCCACGGCC 
TTCGACCCTG CCCCCAGTGC CTGCTCTGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTC 
TCGCTGCAGG ACCTCTTTCC CACCATCGCC TCGGGCTGCT CCTGGACCCT GGAGAACCCA 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGGC AGGAGCAGGT TTGCACACAC 
TTTGCCCCGC GCCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTTACCTG CCTGCGGCCT 
GGTCCAGAGG AAGCCACAGC CCGGGCTGAG TCGGAGGTGG GACGGCCAGA GGAGGAGGAG 
GAGGAGGCGG CGGCAGCAGC ATCAGGGTTG GAGTTGTGTG GTGGCTCAGG CCCCTTTACC 
TTTCTGCACT TCGACAAGAA CTTTGTGCAG CTGTGCCTGT CGGCTGAGCC CTCTGAGGCC 
CCTCGTCTGC TAGCGCCTGC TGCCCTGGCC TTCCGTTTTG TGGAGGTCTT GCTGATCAAC 
AACAACAACT CCAGCCAGTT CACCTGTGGT GTGCTCTGCC GCTGGAGTGA GGAGTGTGGC 
CGGGCTGCAG GCAGGGCTTG TGGCTTTGCA CAGCCAGGGT GTAGTTGTCC TGGGGAGGCA 
GGGGCCAACC CCGCCACCAC CACATCTCCG GGGCCTCCGG TTGCCCACAC CCTGTCCAAT 
GCCCTGGTGC CCGGGGGCCC AGCCCCTCCT GCTGAGGCCG ACTTGCACTC GGGGAGCAGC 
AATGACCTGT TCACCACCGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG 
AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTAT ACATGGCGCA GACAGTGCAC 
GGCGTGTGGG AGGAGTGGGG GTCCTGGAGC CTGTGCTCCC GCAGCTGCGG GCGGGGGTCT 
CGGAGCCGGA TGCGGACCTG CGTGCCCCCC CAGCACGGCG GCAAGGCCTG CGAGGGTCCC 
GAGCTGCAGA CTAAACTCTG CAGTATGGCC GCCTGCCCGG TGGAAGGCCA GTGGCTAGAA 
TGGGGTCCCT GGGGCCCATG CTCATCATCT TGTGCCAATG GGACCCAGCA GCGCAGCCGG 
AAATGCAGTG TGGCGGGTCC AGCCTGGGCC ACGTGCGCAG GTGCCCTCAC GGATACCCGT 
GAGTGCAGCA ATCTCGATTG CCCGGCCACT GACGGCAAGT GGGGGCCGTG GAACGCGTGG 
AGCCTGTGCT CCAAGACGTG TGACACGGGC TGGCAACGCC GCTTCCGCAT GTGCCAGGCT 
TCTGGCACAC AGGGCTACCC TTGCGAGGGC ACAGGAGAGG AGGTGAAACC CTGCAGTGAG 
AAGAGGTGTC CAGCCTTCCA TGAGATGTGC AGGGATGAGT ACGTGATGTT GATGACATGG 
AAGAGGGCGG CAGCTGGCGA GATCATTTAC AACAAGTGTC CCCCTAATGC CTCGGGTTCT 
GCTAGCCGCC GCTGTCTCCT CAGTGCCCAG GGCGTAGCAT ACTGGGGACT GCCCAGCTTT 
GCTCGTTGCA TATCCCATGA ATACCGCTAC CTGTACCTGT CACTTCGGGA ACACCTGGCT 
AAGGGCCAGC GCATGCTGGC AGGTGAGGGC ATGTCACAGG TGGTGCGGAG CCTGCAGGAG 
CTACTGGCAC GGCGCACTTA CTACAGCGGG GACCTGCTCT TCTCTGTGGA CATCCTAAGG 
AACGTCACTG ACACCTTCAA GAGGGCCACC TATGTCCCTT CCGCCGATGA CGTGCAGCGT 
TTCTTCCAGG TGGTGAGCTT CATGGTGGAT TCAGAAAACA AGGACAAATG GGATGATGCT 
CAGCAGGTGT CACCGGGCTC TGTGCACCTG CTGCGTGTTG TGGAAGATTT CATTCACCTC 
GTGGGCGACG CTCTCAAGGC CTTCCAGAGC TCTCTCATTG TCACGGACAA TCTGGTGATC 
AGCATTCAGA GAGAGCCTAT CTCCGCCGTG TCCAGTGACA TCACGTTTCC CATGCGGGGC 
CGCAGGGGCA TGAAGGACTG GGTGCGACAC TCAGAGGATC GTCTCTTTCT ACCCAAGGAG 
GTGCTCAGCC TGTCCTCCCC AGGAAAGCCA GCCACACCTG GGGCAGCCAC AGCAGGCAGC 
CCGGGCAGGG GGAGGGGCCC AGGAACGGTG CCCCCTGGCC CAGGCCACGC CCACCAGCGC 
CTTCTCCCAG CTGACCCCGA AGAGTCCTCC TCCTACTTTG TGATCGGTGC TGTGCTCTAC 
CGCACCCTTG GCCTCATCCT GCCGCCCCCC AGGCCTCCAC TTGCTGTCAC CTCCCGGGTG 
ATGACAGTGA CTGTGCGTCC CCCCACCCAG CCTCCAGCTG AGCCCCTCAT TACAGTGGAA 
CTCTCGTACA TCATCAATGG CACCACCGAT CCCCACTGTG CCAGCTGGGA CTACTCCAGA 
GCAGATACCA ACTCGGGGGA CTGGAACACT GAGAGCTGCC AGACCTTGGA GACCCAGGCG 
GCTCACACCC GCTGCCAGTG CCAGCACCTG TCCACCTTTG CCGTCCTGGC CCAGCCACCC 
AAGGACCTGA CCCTGGAGCT GGCAGGTGCT CCCTCTGTCC CCCTGGTGAT CGGCTGTGCA 
GTGTCCTGCA TGGCTCTGCT CACCCTGCTG GCCATCTATG CAGCCTTCTG GAGGTTTATA 
AAATCAGAAC GCTCCATCAT CTTGCTGAAC TTCTGCCTGT CCATCCTGGC TTCCAACATT 
CTGATCCTGG TGGGCCAGTC CCGGGTGCTG AGCAAGGGCG TATGCACCAT GACGGCTGCC 
TTCCTACACT TCTTCTTTCT GTCCTCCTTT TGCTGGGTGC TTACAGAGGC CTGGCAATCC 
TATCTGGCTG TCATCGGGCG GATGCGCACC CGCCTGGTTC GCAAGCGCTT CCTCTGCCTG 
GGCTGGGGTC TACCTGCCCT GGTGGTGGCT GTGTCTGTTG GCTTTACTCG CACCAAAGGA 
TATGGTACAT CCAGCTACTG CTGGCTGTCC CTAGAGGGCG GCCTGCTCTA TGCCTTCGTG 
GGTCCAGCAG CAGTCATTGT CCTGGTGAAC ATGCTCATCG GGATTATCGT CTTCAACAAG 
CTCATGGCTC GCGATGGCGT CTCAGACAAA TCTAAGAAGC AGAGGGCTGG GGCTTCACTC 
TGGAGTTCCT GCGTGGTACT GCCTCTCCTG GCGCTTACCT GGATGTCTGC CGTCCTGGCC 
ATGACAGATC GCCGCTCCGT CCTCTTCCAG GCACTCTTTG CCGTTTTCAA CTCTGCACAA 
GGCTTTGTCA TCACCGCTGT GCACTGCTTC CTGCGCCGAG AGGTCCAGGA TGTGGTAAAG 
TGTCAGATGG GTGTGTGTCG GGCTGATGAG AGTGAAGACT CCCCAGACTC GTGCAAGAAC 
GGGCAGCTGC AGATCCTGTC AGACTTTGAA AAGGACGTGG ATCTGGCTTG TCAGACAGTT 
CTGTTCAAGG AGGTCAACAC CTGCAACCCG TCCACCATTA CCGGCACTCT GTCCCGCCTG 
TCTCTGGATG AGGATGAGGA GCCCAAGTCC TGTCTCGTGG GTCCTGAGGG TGGCCTCAGC 
TTCTCACCAC TGCCTGGGAA CATCCTGGTA CCCATGGCAG CCTCACCAGG TCTAGGGGAG 
CCACCACCAC CCCAGGAGAC CAACCCTGTG TACATGTGTG GGGAGGGTGG CCTGCGGCAG 
TTGGACCTTA CATGGATACG GCAGAGTGAA CCAGGCTCTG AGGGGGACTA CATGGTTCTG 
CCCCGGCGGA CTTTGAGCCT GCAGCCTGGT GGTGGGGGTA CAGCGGGTGA GGAGGCCCCA 
AGGGCCCGGC CTGAGGGGAC CCCCCGGCGG GCTGCCAAAA CGGTAGCCCA CACTGAAGGC 
TACCCCAGCT TCCTGTCTGT GGAGCACTCG GGTCTAGGGC TGGGCCCTGC CTATGGGTCT 
CTCCAGAACC CGTATGGAAT GACCTTCCAA CCACCACCAC CAACACCCAG CGCCCGCCAA 
GTACCAGAGC CAGGAGAACG TAGCCGGACC ATGCCCCGTA CAGTGCCTGG TTCCACCATG 
AAGCTGGGCT CCCTTGAGCG AAAGAAGCTT CGGTATTCGG ACTTGGACTT TGAGAAGGTG 
ATGCACACTC GGAAACGGCA CTCGGAACTC TACCACGAAC TCAACCAGAA GTTCCACACT 
TTCGACCGCT ACCGTAGCCA GTCCTCAGCC AAGGAGAAAC CCAGCCCCCC CGGGGGACGC 
CCTGGCTTGT CCCAGCACAG GAGGCATCAA AGCTGGAGCA CCTTCAAATC TATGACACTG 
GGCTCACTGC CCCCCAAGCC CCGAGAACGG CTGGCCCTGC ACCGGACAGC AGCCTGGGAG 
CCCACAGAAC CGCCAGACGG CGACTTCCAG ACAGAGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adgrb2 ( NM_001377058.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001363987.1 Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant 7, mRNA.

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CloneID	OMu90667
Clone ID Related Accession (Same CDS sequence)	NM_001377058.1
Accession Version	NM_001377058.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-11
Organism	Mus musculus(house mouse)
Product	adhesion G protein-coupled receptor B2 isoform 7 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL626774.23. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849379 [ECO:0000348] ##Evidence-Data-END##

ATGACCCCAG CCTGTCCCCT CTTACTGTCT GTGATTCTGT CCCTGCGCCT GGCCACGGCC 
TTCGACCCTG CCCCCAGTGC CTGCTCTGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTC 
TCGCTGCAGG ACCTCTTTCC CACCATCGCC TCGGGCTGCT CCTGGACCCT GGAGAACCCA 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGGC AGGAGCAGGT TTGCACACAC 
TTTGCCCCGC GCCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTTACCTG CCTGCGGCCT 
GGTCCAGAGG AAGCCACAGC CCGGGCTGAG TCGGAGGTGG GACGGCCAGA GGAGGAGGAG 
GAGGAGGCGG CGGCAGCAGC ATCAGGGTTG GAGTTGTGTG GTGGCTCAGG CCCCTTTACC 
TTTCTGCACT TCGACAAGAA CTTTGTGCAG CTGTGCCTGT CGGCTGAGCC CTCTGAGGCC 
CCTCGTCTGC TAGCGCCTGC TGCCCTGGCC TTCCGTTTTG TGGAGGTCTT GCTGATCAAC 
AACAACAACT CCAGCCAGTT CACCTGTGGT GTGCTCTGCC GCTGGAGTGA GGAGTGTGGC 
CGGGCTGCAG GCAGGGCTTG TGGCTTTGCA CAGCCAGGGT GTAGTTGTCC TGGGGAGGCA 
GGGGCCAACC CCGCCACCAC CACATCTCCG GGGCCTCCGG TTGCCCACAC CCTGTCCAAT 
GCCCTGGTGC CCGGGGGCCC AGCCCCTCCT GCTGAGGCCG ACTTGCACTC GGGGAGCAGC 
AATGACCTGT TCACCACCGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG 
AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTAT ACATGGCGCA GACAGTGCAC 
GGCGTGTGGG AGGAGTGGGG GTCCTGGAGC CTGTGCTCCC GCAGCTGCGG GCGGGGGTCT 
CGGAGCCGGA TGCGGACCTG CGTGCCCCCC CAGCACGGCG GCAAGGCCTG CGAGGGTCCC 
GAGCTGCAGA CTAAACTCTG CAGTATGGCC GCCTGCCCGG TGGAAGGCCA GTGGCTAGAA 
TGGGGTCCCT GGGGCCCATG CTCATCATCT TGTGCCAATG GGACCCAGCA GCGCAGCCGG 
AAATGCAGTG TGGCGGGTCC AGCCTGGGCC ACGTGCGCAG GTGCCCTCAC GGATACCCGT 
GAGTGCAGCA ATCTCGATTG CCCGGCCACT GACGGCAAGT GGGGGCCGTG GAACGCGTGG 
AGCCTGTGCT CCAAGACGTG TGACACGGGC TGGCAACGCC GCTTCCGCAT GTGCCAGGCT 
TCTGGCACAC AGGGCTACCC TTGCGAGGGC ACAGGAGAGG AGGTGAAACC CTGCAGTGAG 
AAGAGGTGTC CAGCCTTCCA TGAGATGTGC AGGGATGAGT ACGTGATGTT GATGACATGG 
AAGAGGGCGG CAGCTGGCGA GATCATTTAC AACAAGTGTC CCCCTAATGC CTCGGGTTCT 
GCTAGCCGCC GCTGTCTCCT CAGTGCCCAG GGCGTAGCAT ACTGGGGACT GCCCAGCTTT 
GCTCGTTGCA TATCCCATGA ATACCGCTAC CTGTACCTGT CACTTCGGGA ACACCTGGCT 
AAGGGCCAGC GCATGCTGGC AGGTGAGGGC ATGTCACAGG TGGTGCGGAG CCTGCAGGAG 
CTACTGGCAC GGCGCACTTA CTACAGCGGG GACCTGCTCT TCTCTGTGGA CATCCTAAGG 
AACGTCACTG ACACCTTCAA GAGGGCCACC TATGTCCCTT CCGCCGATGA CGTGCAGCGT 
TTCTTCCAGG TGGTGAGCTT CATGGTGGAT TCAGAAAACA AGGACAAATG GGATGATGCT 
CAGCAGGTGT CACCGGGCTC TGTGCACCTG CTGCGTGTTG TGGAAGATTT CATTCACCTC 
GTGGGCGACG CTCTCAAGGC CTTCCAGAGC TCTCTCATTG TCACGGACAA TCTGGTGATC 
AGCATTCAGA GAGAGCCTAT CTCCGCCGTG TCCAGTGACA TCACGTTTCC CATGCGGGGC 
CGCAGGGGCA TGAAGGACTG GGTGCGACAC TCAGAGGATC GTCTCTTTCT ACCCAAGGAG 
GTGCTCAGCC TGTCCTCCCC AGGAAAGCCA GCCACACCTG GGGCAGCCAC AGCAGGCAGC 
CCGGGCAGGG GGAGGGGCCC AGGAACGGTG CCCCCTGGCC CAGGCCACGC CCACCAGCGC 
CTTCTCCCAG CTGACCCCGA AGAGTCCTCC TCCTACTTTG TGATCGGTGC TGTGCTCTAC 
CGCACCCTTG GCCTCATCCT GCCGCCCCCC AGGCCTCCAC TTGCTGTCAC CTCCCGGGTG 
ATGACAGTGA CTGTGCGTCC CCCCACCCAG CCTCCAGCTG AGCCCCTCAT TACAGTGGAA 
CTCTCGTACA TCATCAATGG CACCACCGAT CCCCACTGTG CCAGCTGGGA CTACTCCAGA 
GCAGATACCA ACTCGGGGGA CTGGAACACT GAGAGCTGCC AGACCTTGGA GACCCAGGCG 
GCTCACACCC GCTGCCAGTG CCAGCACCTG TCCACCTTTG CCGTCCTGGC CCAGCCACCC 
AAGGACCTGA CCCTGGAGCT GGCAGGTGCT CCCTCTGTCC CCCTGGTGAT CGGCTGTGCA 
GTGTCCTGCA TGGCTCTGCT CACCCTGCTG GCCATCTATG CAGCCTTCTG GAGGTTTATA 
AAATCAGAAC GCTCCATCAT CTTGCTGAAC TTCTGCCTGT CCATCCTGGC TTCCAACATT 
CTGATCCTGG TGGGCCAGTC CCGGGTGCTG AGCAAGGGCG TATGCACCAT GACGGCTGCC 
TTCCTACACT TCTTCTTTCT GTCCTCCTTT TGCTGGGTGC TTACAGAGGC CTGGCAATCC 
TATCTGGCTG TCATCGGGCG GATGCGCACC CGCCTGGTTC GCAAGCGCTT CCTCTGCCTG 
GGCTGGGGTC TACCTGCCCT GGTGGTGGCT GTGTCTGTTG GCTTTACTCG CACCAAAGGA 
TATGGTACAT CCAGCTACTG CTGGCTGTCC CTAGAGGGCG GCCTGCTCTA TGCCTTCGTG 
GGTCCAGCAG CAGTCATTGT CCTGGTGAAC ATGCTCATCG GGATTATCGT CTTCAACAAG 
CTCATGGCTC GCGATGGCGT CTCAGACAAA TCTAAGAAGC AGAGGGCTGG GGCTTCACTC 
TGGAGTTCCT GCGTGGTACT GCCTCTCCTG GCGCTTACCT GGATGTCTGC CGTCCTGGCC 
ATGACAGATC GCCGCTCCGT CCTCTTCCAG GCACTCTTTG CCGTTTTCAA CTCTGCACAA 
GGCTTTGTCA TCACCGCTGT GCACTGCTTC CTGCGCCGAG AGGTCCAGGA TGTGGTAAAG 
TGTCAGATGG GTGTGTGTCG GGCTGATGAG AGTGAAGACT CCCCAGACTC GTGCAAGAAC 
GGGCAGCTGC AGATCCTGTC AGACTTTGAA AAGGACGTGG ATCTGGCTTG TCAGACAGTT 
CTGTTCAAGG AGGTCAACAC CTGCAACCCG TCCACCATTA CCGGCACTCT GTCCCGCCTG 
TCTCTGGATG AGGATGAGGA GCCCAAGTCC TGTCTCGTGG GTCCTGAGGG TGGCCTCAGC 
TTCTCACCAC TGCCTGGGAA CATCCTGGTA CCCATGGCAG CCTCACCAGG TCTAGGGGAG 
CCACCACCAC CCCAGGAGAC CAACCCTGTG TACATGTGTG GGGAGGGTGG CCTGCGGCAG 
TTGGACCTTA CATGGATACG GCAGAGTGAA CCAGGCTCTG AGGGGGACTA CATGGTTCTG 
CCCCGGCGGA CTTTGAGCCT GCAGCCTGGT GGTGGGGGTA CAGCGGGTGA GGAGGCCCCA 
AGGGCCCGGC CTGAGGGGAC CCCCCGGCGG GCTGCCAAAA CGGTAGCCCA CACTGAAGGC 
TACCCCAGCT TCCTGTCTGT GGAGCACTCG GGTCTAGGGC TGGGCCCTGC CTATGGGTCT 
CTCCAGAACC CGTATGGAAT GACCTTCCAA CCACCACCAC CAACACCCAG CGCCCGCCAA 
GTACCAGAGC CAGGAGAACG TAGCCGGACC ATGCCCCGTA CAGTGCCTGG TTCCACCATG 
AAGCTGGGCT CCCTTGAGCG AAAGAAGCTT CGGTATTCGG ACTTGGACTT TGAGGTGATG 
CACACTCGGA AACGGCACTC GGAACTCTAC CACGAACTCA ACCAGAAGTT CCACACTTTC 
GACCGCTACC GTAGCCAGTC CTCAGCCAAG GAGAAACCCA GCCCCCCCGG GGGACGCCCT 
GGCTTGTCCC AGCACAGGAG GCATCAAAGC TGGAGCACCT TCAAATCTAT GACACTGGGC 
TCACTGCCCC CCAAGCCCCG AGAACGGCTG GCCCTGCACC GGACAGCAGC CTGGGAGCCC 
ACAGAACCGC CAGACGGCGA CTTCCAGACA GAGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363987.1
CDS	454..4872
Translation	MTPACPLLLSVILSLRLATAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCTHFAPRLLPLDHYLVNFTCLRPGPEEATARAESEVGRPEEEEEEAAAAASGLELCGGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGANPATTTSPGPPVAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSSSCANGTQQRSRKCSVAGPAWATCAGALTDTRECSNLDCPATDGKWGPWNAWSLCSKTCDTGWQRRFRMCQASGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKRAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDSENKDKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPISAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSSPGKPATPGAATAGSPGRGRGPGTVPPGPGHAHQRLLPADPEESSSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADTNSGDWNTESCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGAPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNILILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGVSDKSKKQRAGASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGGLSFSPLPGNILVPMAASPGLGEPPPPQETNPVYMCGEGGLRQLDLTWIRQSEPGSEGDYMVLPRRTLSLQPGGGGTAGEEAPRARPEGTPRRAAKTVAHTEGYPSFLSVEHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVPEPGERSRTMPRTVPGSTMKLGSLERKKLRYSDLDFEVMHTRKRHSELYHELNQKFHTFDRYRSQSSAKEKPSPPGGRPGLSQHRRHQSWSTFKSMTLGSLPPKPRERLALHRTAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	NM_001377058.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001377058.1

ORF Insert Sequence:

ATGACCCCAG CCTGTCCCCT CTTACTGTCT GTGATTCTGT CCCTGCGCCT GGCCACGGCC 
TTCGACCCTG CCCCCAGTGC CTGCTCTGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTC 
TCGCTGCAGG ACCTCTTTCC CACCATCGCC TCGGGCTGCT CCTGGACCCT GGAGAACCCA 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGGC AGGAGCAGGT TTGCACACAC 
TTTGCCCCGC GCCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTTACCTG CCTGCGGCCT 
GGTCCAGAGG AAGCCACAGC CCGGGCTGAG TCGGAGGTGG GACGGCCAGA GGAGGAGGAG 
GAGGAGGCGG CGGCAGCAGC ATCAGGGTTG GAGTTGTGTG GTGGCTCAGG CCCCTTTACC 
TTTCTGCACT TCGACAAGAA CTTTGTGCAG CTGTGCCTGT CGGCTGAGCC CTCTGAGGCC 
CCTCGTCTGC TAGCGCCTGC TGCCCTGGCC TTCCGTTTTG TGGAGGTCTT GCTGATCAAC 
AACAACAACT CCAGCCAGTT CACCTGTGGT GTGCTCTGCC GCTGGAGTGA GGAGTGTGGC 
CGGGCTGCAG GCAGGGCTTG TGGCTTTGCA CAGCCAGGGT GTAGTTGTCC TGGGGAGGCA 
GGGGCCAACC CCGCCACCAC CACATCTCCG GGGCCTCCGG TTGCCCACAC CCTGTCCAAT 
GCCCTGGTGC CCGGGGGCCC AGCCCCTCCT GCTGAGGCCG ACTTGCACTC GGGGAGCAGC 
AATGACCTGT TCACCACCGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG 
AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTAT ACATGGCGCA GACAGTGCAC 
GGCGTGTGGG AGGAGTGGGG GTCCTGGAGC CTGTGCTCCC GCAGCTGCGG GCGGGGGTCT 
CGGAGCCGGA TGCGGACCTG CGTGCCCCCC CAGCACGGCG GCAAGGCCTG CGAGGGTCCC 
GAGCTGCAGA CTAAACTCTG CAGTATGGCC GCCTGCCCGG TGGAAGGCCA GTGGCTAGAA 
TGGGGTCCCT GGGGCCCATG CTCATCATCT TGTGCCAATG GGACCCAGCA GCGCAGCCGG 
AAATGCAGTG TGGCGGGTCC AGCCTGGGCC ACGTGCGCAG GTGCCCTCAC GGATACCCGT 
GAGTGCAGCA ATCTCGATTG CCCGGCCACT GACGGCAAGT GGGGGCCGTG GAACGCGTGG 
AGCCTGTGCT CCAAGACGTG TGACACGGGC TGGCAACGCC GCTTCCGCAT GTGCCAGGCT 
TCTGGCACAC AGGGCTACCC TTGCGAGGGC ACAGGAGAGG AGGTGAAACC CTGCAGTGAG 
AAGAGGTGTC CAGCCTTCCA TGAGATGTGC AGGGATGAGT ACGTGATGTT GATGACATGG 
AAGAGGGCGG CAGCTGGCGA GATCATTTAC AACAAGTGTC CCCCTAATGC CTCGGGTTCT 
GCTAGCCGCC GCTGTCTCCT CAGTGCCCAG GGCGTAGCAT ACTGGGGACT GCCCAGCTTT 
GCTCGTTGCA TATCCCATGA ATACCGCTAC CTGTACCTGT CACTTCGGGA ACACCTGGCT 
AAGGGCCAGC GCATGCTGGC AGGTGAGGGC ATGTCACAGG TGGTGCGGAG CCTGCAGGAG 
CTACTGGCAC GGCGCACTTA CTACAGCGGG GACCTGCTCT TCTCTGTGGA CATCCTAAGG 
AACGTCACTG ACACCTTCAA GAGGGCCACC TATGTCCCTT CCGCCGATGA CGTGCAGCGT 
TTCTTCCAGG TGGTGAGCTT CATGGTGGAT TCAGAAAACA AGGACAAATG GGATGATGCT 
CAGCAGGTGT CACCGGGCTC TGTGCACCTG CTGCGTGTTG TGGAAGATTT CATTCACCTC 
GTGGGCGACG CTCTCAAGGC CTTCCAGAGC TCTCTCATTG TCACGGACAA TCTGGTGATC 
AGCATTCAGA GAGAGCCTAT CTCCGCCGTG TCCAGTGACA TCACGTTTCC CATGCGGGGC 
CGCAGGGGCA TGAAGGACTG GGTGCGACAC TCAGAGGATC GTCTCTTTCT ACCCAAGGAG 
GTGCTCAGCC TGTCCTCCCC AGGAAAGCCA GCCACACCTG GGGCAGCCAC AGCAGGCAGC 
CCGGGCAGGG GGAGGGGCCC AGGAACGGTG CCCCCTGGCC CAGGCCACGC CCACCAGCGC 
CTTCTCCCAG CTGACCCCGA AGAGTCCTCC TCCTACTTTG TGATCGGTGC TGTGCTCTAC 
CGCACCCTTG GCCTCATCCT GCCGCCCCCC AGGCCTCCAC TTGCTGTCAC CTCCCGGGTG 
ATGACAGTGA CTGTGCGTCC CCCCACCCAG CCTCCAGCTG AGCCCCTCAT TACAGTGGAA 
CTCTCGTACA TCATCAATGG CACCACCGAT CCCCACTGTG CCAGCTGGGA CTACTCCAGA 
GCAGATACCA ACTCGGGGGA CTGGAACACT GAGAGCTGCC AGACCTTGGA GACCCAGGCG 
GCTCACACCC GCTGCCAGTG CCAGCACCTG TCCACCTTTG CCGTCCTGGC CCAGCCACCC 
AAGGACCTGA CCCTGGAGCT GGCAGGTGCT CCCTCTGTCC CCCTGGTGAT CGGCTGTGCA 
GTGTCCTGCA TGGCTCTGCT CACCCTGCTG GCCATCTATG CAGCCTTCTG GAGGTTTATA 
AAATCAGAAC GCTCCATCAT CTTGCTGAAC TTCTGCCTGT CCATCCTGGC TTCCAACATT 
CTGATCCTGG TGGGCCAGTC CCGGGTGCTG AGCAAGGGCG TATGCACCAT GACGGCTGCC 
TTCCTACACT TCTTCTTTCT GTCCTCCTTT TGCTGGGTGC TTACAGAGGC CTGGCAATCC 
TATCTGGCTG TCATCGGGCG GATGCGCACC CGCCTGGTTC GCAAGCGCTT CCTCTGCCTG 
GGCTGGGGTC TACCTGCCCT GGTGGTGGCT GTGTCTGTTG GCTTTACTCG CACCAAAGGA 
TATGGTACAT CCAGCTACTG CTGGCTGTCC CTAGAGGGCG GCCTGCTCTA TGCCTTCGTG 
GGTCCAGCAG CAGTCATTGT CCTGGTGAAC ATGCTCATCG GGATTATCGT CTTCAACAAG 
CTCATGGCTC GCGATGGCGT CTCAGACAAA TCTAAGAAGC AGAGGGCTGG GGCTTCACTC 
TGGAGTTCCT GCGTGGTACT GCCTCTCCTG GCGCTTACCT GGATGTCTGC CGTCCTGGCC 
ATGACAGATC GCCGCTCCGT CCTCTTCCAG GCACTCTTTG CCGTTTTCAA CTCTGCACAA 
GGCTTTGTCA TCACCGCTGT GCACTGCTTC CTGCGCCGAG AGGTCCAGGA TGTGGTAAAG 
TGTCAGATGG GTGTGTGTCG GGCTGATGAG AGTGAAGACT CCCCAGACTC GTGCAAGAAC 
GGGCAGCTGC AGATCCTGTC AGACTTTGAA AAGGACGTGG ATCTGGCTTG TCAGACAGTT 
CTGTTCAAGG AGGTCAACAC CTGCAACCCG TCCACCATTA CCGGCACTCT GTCCCGCCTG 
TCTCTGGATG AGGATGAGGA GCCCAAGTCC TGTCTCGTGG GTCCTGAGGG TGGCCTCAGC 
TTCTCACCAC TGCCTGGGAA CATCCTGGTA CCCATGGCAG CCTCACCAGG TCTAGGGGAG 
CCACCACCAC CCCAGGAGAC CAACCCTGTG TACATGTGTG GGGAGGGTGG CCTGCGGCAG 
TTGGACCTTA CATGGATACG GCAGAGTGAA CCAGGCTCTG AGGGGGACTA CATGGTTCTG 
CCCCGGCGGA CTTTGAGCCT GCAGCCTGGT GGTGGGGGTA CAGCGGGTGA GGAGGCCCCA 
AGGGCCCGGC CTGAGGGGAC CCCCCGGCGG GCTGCCAAAA CGGTAGCCCA CACTGAAGGC 
TACCCCAGCT TCCTGTCTGT GGAGCACTCG GGTCTAGGGC TGGGCCCTGC CTATGGGTCT 
CTCCAGAACC CGTATGGAAT GACCTTCCAA CCACCACCAC CAACACCCAG CGCCCGCCAA 
GTACCAGAGC CAGGAGAACG TAGCCGGACC ATGCCCCGTA CAGTGCCTGG TTCCACCATG 
AAGCTGGGCT CCCTTGAGCG AAAGAAGCTT CGGTATTCGG ACTTGGACTT TGAGGTGATG 
CACACTCGGA AACGGCACTC GGAACTCTAC CACGAACTCA ACCAGAAGTT CCACACTTTC 
GACCGCTACC GTAGCCAGTC CTCAGCCAAG GAGAAACCCA GCCCCCCCGG GGGACGCCCT 
GGCTTGTCCC AGCACAGGAG GCATCAAAGC TGGAGCACCT TCAAATCTAT GACACTGGGC 
TCACTGCCCC CCAAGCCCCG AGAACGGCTG GCCCTGCACC GGACAGCAGC CTGGGAGCCC 
ACAGAACCGC CAGACGGCGA CTTCCAGACA GAGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adgrb2 ( XM_030253459.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030109319.1 Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OMu73521
Clone ID Related Accession (Same CDS sequence)	XM_011240514.1 , XM_030253459.1
Accession Version	XM_030253459.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	adhesion G protein-coupled receptor B2 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCAG CCTGTCCCCT CTTACTGTCT GTGATTCTGT CCCTGCGCCT GGCCACGGCC 
TTCGACCCTG CCCCCAGTGC CTGCTCTGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTC 
TCGCTGCAGG ACCTCTTTCC CACCATCGCC TCGGGCTGCT CCTGGACCCT GGAGAACCCA 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGGC AGGAGCAGGT TTGCACACAC 
TTTGCCCCGC GCCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTTACCTG CCTGCGGCCT 
GGTCCAGAGG AAGCCACAGC CCGGGCTGAG TCGGAGGTGG GACGGCCAGA GGAGGAGGAG 
GAGGAGGCGG CGGCAGCAGC ATCAGGGTTG GAGTTGTGTG GTGGCTCAGG CCCCTTTACC 
TTTCTGCACT TCGACAAGAA CTTTGTGCAG CTGTGCCTGT CGGCTGAGCC CTCTGAGGCC 
CCTCGTCTGC TAGCGCCTGC TGCCCTGGCC TTCCGTTTTG TGGAGGTCTT GCTGATCAAC 
AACAACAACT CCAGCCAGTT CACCTGTGGT GTGCTCTGCC GCTGGAGTGA GGAGTGTGGC 
CGGGCTGCAG GCAGGGCTTG TGGCTTTGCA CAGCCAGGGT GTAGTTGTCC TGGGGAGGCA 
GGGGCCAACC CCGCCACCAC CACATCTCCG GGGCCTCCGG TTGCCCACAC CCTGTCCAAT 
GCCCTGGTGC CCGGGGGCCC AGCCCCTCCT GCTGAGGCCG ACTTGCACTC GGGGAGCAGC 
AATGACCTGT TCACCACCGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG 
AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTAT ACATGGCGCA GACAGTGGAA 
GGCCAGTGGC TAGAATGGGG TCCCTGGGGC CCATGCTCAT CATCTTGTGC CAATGGGACC 
CAGCAGCGCA GCCGGAAATG CAGTGTGGCG GGTCCAGCCT GGGCCACGTG CGCAGGTGCC 
CTCACGGATA CCCGTGAGTG CAGCAATCTC GATTGCCCGG CCACTGACGG CAAGTGGGGG 
CCGTGGAACG CGTGGAGCCT GTGCTCCAAG ACGTGTGACA CGGGCTGGCA ACGCCGCTTC 
CGCATGTGCC AGGCTTCTGG CACACAGGGC TACCCTTGCG AGGGCACAGG AGAGGAGGTG 
AAACCCTGCA GTGAGAAGAG GTGTCCAGCC TTCCATGAGA TGTGCAGGGA TGAGTACGTG 
ATGTTGATGA CATGGAAGAG GGCGGCAGCT GGCGAGATCA TTTACAACAA GTGTCCCCCT 
AATGCCTCGG GTTCTGCTAG CCGCCGCTGT CTCCTCAGTG CCCAGGGCGT AGCATACTGG 
GGACTGCCCA GCTTTGCTCG TTGCATATCC CATGAATACC GCTACCTGTA CCTGTCACTT 
CGGGAACACC TGGCTAAGGG CCAGCGCATG CTGGCAGGTG AGGGCATGTC ACAGGTGGTG 
CGGAGCCTGC AGGAGCTACT GGCACGGCGC ACTTACTACA GCGGGGACCT GCTCTTCTCT 
GTGGACATCC TAAGGAACGT CACTGACACC TTCAAGAGGG CCACCTATGT CCCTTCCGCC 
GATGACGTGC AGCGTTTCTT CCAGGTGGTG AGCTTCATGG TGGATTCAGA AAACAAGGAC 
AAATGGGATG ATGCTCAGCA GGTGTCACCG GGCTCTGTGC ACCTGCTGCG TGTTGTGGAA 
GATTTCATTC ACCTCGTGGG CGACGCTCTC AAGGCCTTCC AGAGCTCTCT CATTGTCACG 
GACAATCTGG TGATCAGCAT TCAGAGAGAG CCTATCTCCG CCGTGTCCAG TGACATCACG 
TTTCCCATGC GGGGCCGCAG GGGCATGAAG GACTGGGTGC GACACTCAGA GGATCGTCTC 
TTTCTACCCA AGGAGGTGCT CAGCCTGTCC TCCCCAGGAA AGCCAGCCAC ACCTGGGGCA 
GCCACAGCAG GCAGCCCGGG CAGGGGGAGG GGCCCAGGAA CGGTGCCCCC TGGCCCAGGC 
CACGCCCACC AGCGCCTTCT CCCAGCTGAC CCCGAAGAGT CCTCCTCCTA CTTTGTGATC 
GGTGCTGTGC TCTACCGCAC CCTTGGCCTC ATCCTGCCGC CCCCCAGGCC TCCACTTGCT 
GTCACCTCCC GGGTGATGAC AGTGACTGTG CGTCCCCCCA CCCAGCCTCC AGCTGAGCCC 
CTCATTACAG TGGAACTCTC GTACATCATC AATGGCACCA CCGATCCCCA CTGTGCCAGC 
TGGGACTACT CCAGAGCAGA TACCAACTCG GGGGACTGGA ACACTGAGAG CTGCCAGACC 
TTGGAGACCC AGGCGGCTCA CACCCGCTGC CAGTGCCAGC ACCTGTCCAC CTTTGCCGTC 
CTGGCCCAGC CACCCAAGGA CCTGACCCTG GAGCTGGCAG GTGCTCCCTC TGTCCCCCTG 
GTGATCGGCT GTGCAGTGTC CTGCATGGCT CTGCTCACCC TGCTGGCCAT CTATGCAGCC 
TTCTGGAGGT TTATAAAATC AGAACGCTCC ATCATCTTGC TGAACTTCTG CCTGTCCATC 
CTGGCTTCCA ACATTCTGAT CCTGGTGGGC CAGTCCCGGG TGCTGAGCAA GGGCGTATGC 
ACCATGACGG CTGCCTTCCT ACACTTCTTC TTTCTGTCCT CCTTTTGCTG GGTGCTTACA 
GAGGCCTGGC AATCCTATCT GGCTGTCATC GGGCGGATGC GCACCCGCCT GGTTCGCAAG 
CGCTTCCTCT GCCTGGGCTG GGGTCTACCT GCCCTGGTGG TGGCTGTGTC TGTTGGCTTT 
ACTCGCACCA AAGGATATGG TACATCCAGC TACTGCTGGC TGTCCCTAGA GGGCGGCCTG 
CTCTATGCCT TCGTGGGTCC AGCAGCAGTC ATTGTCCTGG TGAACATGCT CATCGGGATT 
ATCGTCTTCA ACAAGCTCAT GGCTCGCGAT GGCGTCTCAG ACAAATCTAA GAAGCAGAGG 
GCTGGGTCGG AGCGGTGCCC CTGGGCCAGC CTGCTCCTTC CCTGCTCAGC GTGTGGAGCG 
GTCCCCAGCC CCCTGCTCAG CTCAGCCTCG GCCAGGAATG CCATCCGACT GTCTTGGAAT 
CTCTGGGGAT ATGGCTCTCA ACTGTGTCTT TTTCCAAAGC TTCCAAGGGC TTCACTCTGG 
AGTTCCTGCG TGGTACTGCC TCTCCTGGCG CTTACCTGGA TGTCTGCCGT CCTGGCCATG 
ACAGATCGCC GCTCCGTCCT CTTCCAGGCA CTCTTTGCCG TTTTCAACTC TGCACAAGGC 
TTTGTCATCA CCGCTGTGCA CTGCTTCCTG CGCCGAGAGG TCCAGGATGT GGTAAAGTGT 
CAGATGGGTG TGTGTCGGGC TGATGAGAGT GAAGACTCCC CAGACTCGTG CAAGAACGGG 
CAGCTGCAGA TCCTGTCAGA CTTTGAAAAG GACGTGGATC TGGCTTGTCA GACAGTTCTG 
TTCAAGGAGG TCAACACCTG CAACCCGTCC ACCATTACCG GCACTCTGTC CCGCCTGTCT 
CTGGATGAGG ATGAGGAGCC CAAGTCCTGT CTCGTGGGTC CTGAGGGTGG CCTCAGCTTC 
TCACCACTGC CTGGGAACAT CCTGGTACCC ATGGCAGCCT CACCAGGTCT AGGGGAGCCA 
CCACCACCCC AGGAGACCAA CCCTGTGTAC ATGTGTGGGG AGGGTGGCCT GCGGCAGTTG 
GACCTTACAT GGATACGGCA GAGTGAACCA GGCTCTGAGG GGGACTACAT GGTTCTGCCC 
CGGCGGACTT TGAGCCTGCA GCCTGGTGGT GGGGGTACAG CGGGTGAGGA GGCCCCAAGG 
GCCCGGCCTG AGGGGACCCC CCGGCGGGCT GCCAAAACGG TAGCCCACAC TGAAGGCTAC 
CCCAGCTTCC TGTCTGTGGA GCACTCGGGT CTAGGGCTGG GCCCTGCCTA TGGGTCTCTC 
CAGAACCCGT ATGGAATGAC CTTCCAACCA CCACCACCAA CACCCAGCGC CCGCCAAGTA 
CCAGAGCCAG GAGAACGTAG CCGGACCATG CCCCGTACAG TGCCTGGTTC CACCATGAAG 
CTGGGCTCCC TTGAGCGAAA GAAGCTTCGG TATTCGGACT TGGACTTTGA GAAGGTGATG 
CACACTCGGA AACGGCACTC GGAACTCTAC CACGAACTCA ACCAGAAGTT CCACACTTTC 
GACCGCTACC GTAGCCAGTC CTCAGCCAAG GAGAAACCCA GCCCCCCCGG GGGACGCCCT 
GGCTTGTCCC AGCACAGGAG GCATCAAAGC TGGAGCACCT TCAAATCTAT GACACTGGGC 
TCACTGCCCC CCAAGCCCCG AGAACGGCTG GCCCTGCACC GGACAGCAGC CTGGGAGCCC 
ACAGAACCGC CAGACGGCGA CTTCCAGACA GAGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030109319.1
CDS	551..4969
Translation	MTPACPLLLSVILSLRLATAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCTHFAPRLLPLDHYLVNFTCLRPGPEEATARAESEVGRPEEEEEEAAAAASGLELCGGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGANPATTTSPGPPVAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTVEGQWLEWGPWGPCSSSCANGTQQRSRKCSVAGPAWATCAGALTDTRECSNLDCPATDGKWGPWNAWSLCSKTCDTGWQRRFRMCQASGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKRAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDSENKDKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPISAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSSPGKPATPGAATAGSPGRGRGPGTVPPGPGHAHQRLLPADPEESSSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADTNSGDWNTESCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGAPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNILILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGVSDKSKKQRAGSERCPWASLLLPCSACGAVPSPLLSSASARNAIRLSWNLWGYGSQLCLFPKLPRASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGGLSFSPLPGNILVPMAASPGLGEPPPPQETNPVYMCGEGGLRQLDLTWIRQSEPGSEGDYMVLPRRTLSLQPGGGGTAGEEAPRARPEGTPRRAAKTVAHTEGYPSFLSVEHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVPEPGERSRTMPRTVPGSTMKLGSLERKKLRYSDLDFEKVMHTRKRHSELYHELNQKFHTFDRYRSQSSAKEKPSPPGGRPGLSQHRRHQSWSTFKSMTLGSLPPKPRERLALHRTAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_030253459.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030253459.1

ORF Insert Sequence:

ATGACCCCAG CCTGTCCCCT CTTACTGTCT GTGATTCTGT CCCTGCGCCT GGCCACGGCC 
TTCGACCCTG CCCCCAGTGC CTGCTCTGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTC 
TCGCTGCAGG ACCTCTTTCC CACCATCGCC TCGGGCTGCT CCTGGACCCT GGAGAACCCA 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGGC AGGAGCAGGT TTGCACACAC 
TTTGCCCCGC GCCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTTACCTG CCTGCGGCCT 
GGTCCAGAGG AAGCCACAGC CCGGGCTGAG TCGGAGGTGG GACGGCCAGA GGAGGAGGAG 
GAGGAGGCGG CGGCAGCAGC ATCAGGGTTG GAGTTGTGTG GTGGCTCAGG CCCCTTTACC 
TTTCTGCACT TCGACAAGAA CTTTGTGCAG CTGTGCCTGT CGGCTGAGCC CTCTGAGGCC 
CCTCGTCTGC TAGCGCCTGC TGCCCTGGCC TTCCGTTTTG TGGAGGTCTT GCTGATCAAC 
AACAACAACT CCAGCCAGTT CACCTGTGGT GTGCTCTGCC GCTGGAGTGA GGAGTGTGGC 
CGGGCTGCAG GCAGGGCTTG TGGCTTTGCA CAGCCAGGGT GTAGTTGTCC TGGGGAGGCA 
GGGGCCAACC CCGCCACCAC CACATCTCCG GGGCCTCCGG TTGCCCACAC CCTGTCCAAT 
GCCCTGGTGC CCGGGGGCCC AGCCCCTCCT GCTGAGGCCG ACTTGCACTC GGGGAGCAGC 
AATGACCTGT TCACCACCGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG 
AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTAT ACATGGCGCA GACAGTGGAA 
GGCCAGTGGC TAGAATGGGG TCCCTGGGGC CCATGCTCAT CATCTTGTGC CAATGGGACC 
CAGCAGCGCA GCCGGAAATG CAGTGTGGCG GGTCCAGCCT GGGCCACGTG CGCAGGTGCC 
CTCACGGATA CCCGTGAGTG CAGCAATCTC GATTGCCCGG CCACTGACGG CAAGTGGGGG 
CCGTGGAACG CGTGGAGCCT GTGCTCCAAG ACGTGTGACA CGGGCTGGCA ACGCCGCTTC 
CGCATGTGCC AGGCTTCTGG CACACAGGGC TACCCTTGCG AGGGCACAGG AGAGGAGGTG 
AAACCCTGCA GTGAGAAGAG GTGTCCAGCC TTCCATGAGA TGTGCAGGGA TGAGTACGTG 
ATGTTGATGA CATGGAAGAG GGCGGCAGCT GGCGAGATCA TTTACAACAA GTGTCCCCCT 
AATGCCTCGG GTTCTGCTAG CCGCCGCTGT CTCCTCAGTG CCCAGGGCGT AGCATACTGG 
GGACTGCCCA GCTTTGCTCG TTGCATATCC CATGAATACC GCTACCTGTA CCTGTCACTT 
CGGGAACACC TGGCTAAGGG CCAGCGCATG CTGGCAGGTG AGGGCATGTC ACAGGTGGTG 
CGGAGCCTGC AGGAGCTACT GGCACGGCGC ACTTACTACA GCGGGGACCT GCTCTTCTCT 
GTGGACATCC TAAGGAACGT CACTGACACC TTCAAGAGGG CCACCTATGT CCCTTCCGCC 
GATGACGTGC AGCGTTTCTT CCAGGTGGTG AGCTTCATGG TGGATTCAGA AAACAAGGAC 
AAATGGGATG ATGCTCAGCA GGTGTCACCG GGCTCTGTGC ACCTGCTGCG TGTTGTGGAA 
GATTTCATTC ACCTCGTGGG CGACGCTCTC AAGGCCTTCC AGAGCTCTCT CATTGTCACG 
GACAATCTGG TGATCAGCAT TCAGAGAGAG CCTATCTCCG CCGTGTCCAG TGACATCACG 
TTTCCCATGC GGGGCCGCAG GGGCATGAAG GACTGGGTGC GACACTCAGA GGATCGTCTC 
TTTCTACCCA AGGAGGTGCT CAGCCTGTCC TCCCCAGGAA AGCCAGCCAC ACCTGGGGCA 
GCCACAGCAG GCAGCCCGGG CAGGGGGAGG GGCCCAGGAA CGGTGCCCCC TGGCCCAGGC 
CACGCCCACC AGCGCCTTCT CCCAGCTGAC CCCGAAGAGT CCTCCTCCTA CTTTGTGATC 
GGTGCTGTGC TCTACCGCAC CCTTGGCCTC ATCCTGCCGC CCCCCAGGCC TCCACTTGCT 
GTCACCTCCC GGGTGATGAC AGTGACTGTG CGTCCCCCCA CCCAGCCTCC AGCTGAGCCC 
CTCATTACAG TGGAACTCTC GTACATCATC AATGGCACCA CCGATCCCCA CTGTGCCAGC 
TGGGACTACT CCAGAGCAGA TACCAACTCG GGGGACTGGA ACACTGAGAG CTGCCAGACC 
TTGGAGACCC AGGCGGCTCA CACCCGCTGC CAGTGCCAGC ACCTGTCCAC CTTTGCCGTC 
CTGGCCCAGC CACCCAAGGA CCTGACCCTG GAGCTGGCAG GTGCTCCCTC TGTCCCCCTG 
GTGATCGGCT GTGCAGTGTC CTGCATGGCT CTGCTCACCC TGCTGGCCAT CTATGCAGCC 
TTCTGGAGGT TTATAAAATC AGAACGCTCC ATCATCTTGC TGAACTTCTG CCTGTCCATC 
CTGGCTTCCA ACATTCTGAT CCTGGTGGGC CAGTCCCGGG TGCTGAGCAA GGGCGTATGC 
ACCATGACGG CTGCCTTCCT ACACTTCTTC TTTCTGTCCT CCTTTTGCTG GGTGCTTACA 
GAGGCCTGGC AATCCTATCT GGCTGTCATC GGGCGGATGC GCACCCGCCT GGTTCGCAAG 
CGCTTCCTCT GCCTGGGCTG GGGTCTACCT GCCCTGGTGG TGGCTGTGTC TGTTGGCTTT 
ACTCGCACCA AAGGATATGG TACATCCAGC TACTGCTGGC TGTCCCTAGA GGGCGGCCTG 
CTCTATGCCT TCGTGGGTCC AGCAGCAGTC ATTGTCCTGG TGAACATGCT CATCGGGATT 
ATCGTCTTCA ACAAGCTCAT GGCTCGCGAT GGCGTCTCAG ACAAATCTAA GAAGCAGAGG 
GCTGGGTCGG AGCGGTGCCC CTGGGCCAGC CTGCTCCTTC CCTGCTCAGC GTGTGGAGCG 
GTCCCCAGCC CCCTGCTCAG CTCAGCCTCG GCCAGGAATG CCATCCGACT GTCTTGGAAT 
CTCTGGGGAT ATGGCTCTCA ACTGTGTCTT TTTCCAAAGC TTCCAAGGGC TTCACTCTGG 
AGTTCCTGCG TGGTACTGCC TCTCCTGGCG CTTACCTGGA TGTCTGCCGT CCTGGCCATG 
ACAGATCGCC GCTCCGTCCT CTTCCAGGCA CTCTTTGCCG TTTTCAACTC TGCACAAGGC 
TTTGTCATCA CCGCTGTGCA CTGCTTCCTG CGCCGAGAGG TCCAGGATGT GGTAAAGTGT 
CAGATGGGTG TGTGTCGGGC TGATGAGAGT GAAGACTCCC CAGACTCGTG CAAGAACGGG 
CAGCTGCAGA TCCTGTCAGA CTTTGAAAAG GACGTGGATC TGGCTTGTCA GACAGTTCTG 
TTCAAGGAGG TCAACACCTG CAACCCGTCC ACCATTACCG GCACTCTGTC CCGCCTGTCT 
CTGGATGAGG ATGAGGAGCC CAAGTCCTGT CTCGTGGGTC CTGAGGGTGG CCTCAGCTTC 
TCACCACTGC CTGGGAACAT CCTGGTACCC ATGGCAGCCT CACCAGGTCT AGGGGAGCCA 
CCACCACCCC AGGAGACCAA CCCTGTGTAC ATGTGTGGGG AGGGTGGCCT GCGGCAGTTG 
GACCTTACAT GGATACGGCA GAGTGAACCA GGCTCTGAGG GGGACTACAT GGTTCTGCCC 
CGGCGGACTT TGAGCCTGCA GCCTGGTGGT GGGGGTACAG CGGGTGAGGA GGCCCCAAGG 
GCCCGGCCTG AGGGGACCCC CCGGCGGGCT GCCAAAACGG TAGCCCACAC TGAAGGCTAC 
CCCAGCTTCC TGTCTGTGGA GCACTCGGGT CTAGGGCTGG GCCCTGCCTA TGGGTCTCTC 
CAGAACCCGT ATGGAATGAC CTTCCAACCA CCACCACCAA CACCCAGCGC CCGCCAAGTA 
CCAGAGCCAG GAGAACGTAG CCGGACCATG CCCCGTACAG TGCCTGGTTC CACCATGAAG 
CTGGGCTCCC TTGAGCGAAA GAAGCTTCGG TATTCGGACT TGGACTTTGA GAAGGTGATG 
CACACTCGGA AACGGCACTC GGAACTCTAC CACGAACTCA ACCAGAAGTT CCACACTTTC 
GACCGCTACC GTAGCCAGTC CTCAGCCAAG GAGAAACCCA GCCCCCCCGG GGGACGCCCT 
GGCTTGTCCC AGCACAGGAG GCATCAAAGC TGGAGCACCT TCAAATCTAT GACACTGGGC 
TCACTGCCCC CCAAGCCCCG AGAACGGCTG GCCCTGCACC GGACAGCAGC CTGGGAGCCC 
ACAGAACCGC CAGACGGCGA CTTCCAGACA GAGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adgrb2 ( XM_030253460.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030109320.1 Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant X10, mRNA.

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CloneID	OMu88509
Clone ID Related Accession (Same CDS sequence)	XM_030253460.1
Accession Version	XM_030253460.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	adhesion G protein-coupled receptor B2 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCAG CCTGTCCCCT CTTACTGTCT GTGATTCTGT CCCTGCGCCT GGCCACGGCC 
TTCGACCCTG CCCCCAGTGC CTGCTCTGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTC 
TCGCTGCAGG ACCTCTTTCC CACCATCGCC TCGGGCTGCT CCTGGACCCT GGAGAACCCA 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGGC AGGAGCAGGT TTGCACACAC 
TTTGCCCCGC GCCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTTACCTG CCTGCGGCCT 
GGTCCAGAGG AAGCCACAGC CCGGGCTGAG TCGGAGGTGG GACGGCCAGA GGAGGAGGAG 
GAGGAGGCGG CGGCAGCAGC ATCAGGGTTG GAGTTGTGTG GTGGCTCAGG CCCCTTTACC 
TTTCTGCACT TCGACAAGAA CTTTGTGCAG CTGTGCCTGT CGGCTGAGCC CTCTGAGGCC 
CCTCGTCTGC TAGCGCCTGC TGCCCTGGCC TTCCGTTTTG TGGAGGTCTT GCTGATCAAC 
AACAACAACT CCAGCCAGTT CACCTGTGGT GTGCTCTGCC GCTGGAGTGA GGAGTGTGGC 
CGGGCTGCAG GCAGGGCTTG TGGCTTTGCA CAGCCAGGGT GTAGTTGTCC TGGGGAGGCA 
GGGGCCAACC CCGCCACCAC CACATCTCCG GGGCCTCCGG TTGCCCACAC CCTGTCCAAT 
GCCCTGGTGC CCGGGGGCCC AGCCCCTCCT GCTGAGGCCG ACTTGCACTC GGGGAGCAGC 
AATGACCTGT TCACCACCGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG 
AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTAT ACATGGCGCA GACAGTGGAA 
GGCCAGTGGC TAGAATGGGG TCCCTGGGGC CCATGCTCAT CATCTTGTGC CAATGGGACC 
CAGCAGCGCA GCCGGAAATG CAGTGTGGCG GGTCCAGCCT GGGCCACGTG CGCAGGTGCC 
CTCACGGATA CCCGTGAGTG CAGCAATCTC GATTGCCCGG CCACTGACGG CAAGTGGGGG 
CCGTGGAACG CGTGGAGCCT GTGCTCCAAG ACGTGTGACA CGGGCTGGCA ACGCCGCTTC 
CGCATGTGCC AGGCTTCTGG CACACAGGGC TACCCTTGCG AGGGCACAGG AGAGGAGGTG 
AAACCCTGCA GTGAGAAGAG GTGTCCAGCC TTCCATGAGA TGTGCAGGGA TGAGTACGTG 
ATGTTGATGA CATGGAAGAG GGCGGCAGCT GGCGAGATCA TTTACAACAA GTGTCCCCCT 
AATGCCTCGG GTTCTGCTAG CCGCCGCTGT CTCCTCAGTG CCCAGGGCGT AGCATACTGG 
GGACTGCCCA GCTTTGCTCG TTGCATATCC CATGAATACC GCTACCTGTA CCTGTCACTT 
CGGGAACACC TGGCTAAGGG CCAGCGCATG CTGGCAGGTG AGGGCATGTC ACAGGTGGTG 
CGGAGCCTGC AGGAGCTACT GGCACGGCGC ACTTACTACA GCGGGGACCT GCTCTTCTCT 
GTGGACATCC TAAGGAACGT CACTGACACC TTCAAGAGGG CCACCTATGT CCCTTCCGCC 
GATGACGTGC AGCGTTTCTT CCAGGTGGTG AGCTTCATGG TGGATTCAGA AAACAAGGAC 
AAATGGGATG ATGCTCAGCA GGTGTCACCG GGCTCTGTGC ACCTGCTGCG TGTTGTGGAA 
GATTTCATTC ACCTCGTGGG CGACGCTCTC AAGGCCTTCC AGAGCTCTCT CATTGTCACG 
GACAATCTGG TGATCAGCAT TCAGAGAGAG CCTATCTCCG CCGTGTCCAG TGACATCACG 
TTTCCCATGC GGGGCCGCAG GGGCATGAAG GACTGGGTGC GACACTCAGA GGATCGTCTC 
TTTCTACCCA AGGAGGTGCT CAGCCTGTCC TCCCCAGGAA AGCCAGCCAC ACCTGGGGCA 
GCCACAGCAG GCAGCCCGGG CAGGGGGAGG GGCCCAGGAA CGGTGCCCCC TGGCCCAGGC 
CACGCCCACC AGCGCCTTCT CCCAGCTGAC CCCGAAGAGT CCTCCTCCTA CTTTGTGATC 
GGTGCTGTGC TCTACCGCAC CCTTGGCCTC ATCCTGCCGC CCCCCAGGCC TCCACTTGCT 
GTCACCTCCC GGGTGATGAC AGTGACTGTG CGTCCCCCCA CCCAGCCTCC AGCTGAGCCC 
CTCATTACAG TGGAACTCTC GTACATCATC AATGGCACCA CCGATCCCCA CTGTGCCAGC 
TGGGACTACT CCAGAGCAGA TACCAACTCG GGGGACTGGA ACACTGAGAG CTGCCAGACC 
TTGGAGACCC AGGCGGCTCA CACCCGCTGC CAGTGCCAGC ACCTGTCCAC CTTTGCCGTC 
CTGGCCCAGC CACCCAAGGA CCTGACCCTG GAGCTGGCAG GTGCTCCCTC TGTCCCCCTG 
GTGATCGGCT GTGCAGTGTC CTGCATGGCT CTGCTCACCC TGCTGGCCAT CTATGCAGCC 
TTCTGGAGGT TTATAAAATC AGAACGCTCC ATCATCTTGC TGAACTTCTG CCTGTCCATC 
CTGGCTTCCA ACATTCTGAT CCTGGTGGGC CAGTCCCGGG TGCTGAGCAA GGGCGTATGC 
ACCATGACGG CTGCCTTCCT ACACTTCTTC TTTCTGTCCT CCTTTTGCTG GGTGCTTACA 
GAGGCCTGGC AATCCTATCT GGCTGTCATC GGGCGGATGC GCACCCGCCT GGTTCGCAAG 
CGCTTCCTCT GCCTGGGCTG GGGTCTACCT GCCCTGGTGG TGGCTGTGTC TGTTGGCTTT 
ACTCGCACCA AAGGATATGG TACATCCAGC TACTGCTGGC TGTCCCTAGA GGGCGGCCTG 
CTCTATGCCT TCGTGGGTCC AGCAGCAGTC ATTGTCCTGG TGAACATGCT CATCGGGATT 
ATCGTCTTCA ACAAGCTCAT GGCTCGCGAT GGCGTCTCAG ACAAATCTAA GAAGCAGAGG 
GCTGGGGCTT CACTCTGGAG TTCCTGCGTG GTACTGCCTC TCCTGGCGCT TACCTGGATG 
TCTGCCGTCC TGGCCATGAC AGATCGCCGC TCCGTCCTCT TCCAGGCACT CTTTGCCGTT 
TTCAACTCTG CACAAGGCTT TGTCATCACC GCTGTGCACT GCTTCCTGCG CCGAGAGGTC 
CAGGATGTGG TAAAGTGTCA GATGGGTGTG TGTCGGGCTG ATGAGAGTGA AGACTCCCCA 
GACTCGTGCA AGAACGGGCA GCTGCAGATC CTGTCAGACT TTGAAAAGGA CGTGGATCTG 
GCTTGTCAGA CAGTTCTGTT CAAGGAGGTC AACACCTGCA ACCCGTCCAC CATTACCGGC 
ACTCTGTCCC GCCTGTCTCT GGATGAGGAT GAGGAGCCCA AGTCCTGTCT CGTGGGTCCT 
GAGGGTGGCC TCAGCTTCTC ACCACTGCCT GGGAACATCC TGGTACCCAT GGCAGCCTCA 
CCAGGTCTAG GGGAGCCACC ACCACCCCAG GAGACCAACC CTGTGTACAT GTGTGGGGAG 
GGTGGCCTGC GGCAGTTGGA CCTTACATGG ATACGGCAGA GTGAACCAGG CTCTGAGGGG 
GACTACATGG TTCTGCCCCG GCGGACTTTG AGCCTGCAGC CTGGTGGTGG GGGTACAGCG 
GGTGAGGAGG CCCCAAGGGC CCGGCCTGAG GGGACCCCCC GGCGGGCTGC CAAAACGGTA 
GCCCACACTG AAGGCTACCC CAGCTTCCTG TCTGTGGAGC ACTCGGGTCT AGGGCTGGGC 
CCTGCCTATG GGTCTCTCCA GAACCCGTAT GGAATGACCT TCCAACCACC ACCACCAACA 
CCCAGCGCCC GCCAAGTACC AGAGCCAGGA GAACGTAGCC GGACCATGCC CCGTACAGTG 
CCTGGTTCCA CCATGAAGCT GGGCTCCCTT GAGCGAAAGA AGCTTCGGTA TTCGGACTTG 
GACTTTGAGA AGGTGATGCA CACTCGGAAA CGGCACTCGG AACTCTACCA CGAACTCAAC 
CAGAAGTTCC ACACTTTCGA CCGCTACCGT AGCCAGTCCT CAGCCAAGGA GAAACCCAGC 
CCCCCCGGGG GACGCCCTGG CTTGTCCCAG CACAGGAGGC ATCAAAGCTG GAGCACCTTC 
AAATCTATGA CACTGGGCTC ACTGCCCCCC AAGCCCCGAG AACGGCTGGC CCTGCACCGG 
ACAGCAGCCT GGGAGCCCAC AGAACCGCCA GACGGCGACT TCCAGACAGA GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030109320.1
CDS	551..4807
Translation	MTPACPLLLSVILSLRLATAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCTHFAPRLLPLDHYLVNFTCLRPGPEEATARAESEVGRPEEEEEEAAAAASGLELCGGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGANPATTTSPGPPVAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTVEGQWLEWGPWGPCSSSCANGTQQRSRKCSVAGPAWATCAGALTDTRECSNLDCPATDGKWGPWNAWSLCSKTCDTGWQRRFRMCQASGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKRAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDSENKDKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPISAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSSPGKPATPGAATAGSPGRGRGPGTVPPGPGHAHQRLLPADPEESSSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADTNSGDWNTESCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGAPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNILILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGVSDKSKKQRAGASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGGLSFSPLPGNILVPMAASPGLGEPPPPQETNPVYMCGEGGLRQLDLTWIRQSEPGSEGDYMVLPRRTLSLQPGGGGTAGEEAPRARPEGTPRRAAKTVAHTEGYPSFLSVEHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVPEPGERSRTMPRTVPGSTMKLGSLERKKLRYSDLDFEKVMHTRKRHSELYHELNQKFHTFDRYRSQSSAKEKPSPPGGRPGLSQHRRHQSWSTFKSMTLGSLPPKPRERLALHRTAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_030253460.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030253460.1

ORF Insert Sequence:

ATGACCCCAG CCTGTCCCCT CTTACTGTCT GTGATTCTGT CCCTGCGCCT GGCCACGGCC 
TTCGACCCTG CCCCCAGTGC CTGCTCTGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTC 
TCGCTGCAGG ACCTCTTTCC CACCATCGCC TCGGGCTGCT CCTGGACCCT GGAGAACCCA 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGGC AGGAGCAGGT TTGCACACAC 
TTTGCCCCGC GCCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTTACCTG CCTGCGGCCT 
GGTCCAGAGG AAGCCACAGC CCGGGCTGAG TCGGAGGTGG GACGGCCAGA GGAGGAGGAG 
GAGGAGGCGG CGGCAGCAGC ATCAGGGTTG GAGTTGTGTG GTGGCTCAGG CCCCTTTACC 
TTTCTGCACT TCGACAAGAA CTTTGTGCAG CTGTGCCTGT CGGCTGAGCC CTCTGAGGCC 
CCTCGTCTGC TAGCGCCTGC TGCCCTGGCC TTCCGTTTTG TGGAGGTCTT GCTGATCAAC 
AACAACAACT CCAGCCAGTT CACCTGTGGT GTGCTCTGCC GCTGGAGTGA GGAGTGTGGC 
CGGGCTGCAG GCAGGGCTTG TGGCTTTGCA CAGCCAGGGT GTAGTTGTCC TGGGGAGGCA 
GGGGCCAACC CCGCCACCAC CACATCTCCG GGGCCTCCGG TTGCCCACAC CCTGTCCAAT 
GCCCTGGTGC CCGGGGGCCC AGCCCCTCCT GCTGAGGCCG ACTTGCACTC GGGGAGCAGC 
AATGACCTGT TCACCACCGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG 
AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTAT ACATGGCGCA GACAGTGGAA 
GGCCAGTGGC TAGAATGGGG TCCCTGGGGC CCATGCTCAT CATCTTGTGC CAATGGGACC 
CAGCAGCGCA GCCGGAAATG CAGTGTGGCG GGTCCAGCCT GGGCCACGTG CGCAGGTGCC 
CTCACGGATA CCCGTGAGTG CAGCAATCTC GATTGCCCGG CCACTGACGG CAAGTGGGGG 
CCGTGGAACG CGTGGAGCCT GTGCTCCAAG ACGTGTGACA CGGGCTGGCA ACGCCGCTTC 
CGCATGTGCC AGGCTTCTGG CACACAGGGC TACCCTTGCG AGGGCACAGG AGAGGAGGTG 
AAACCCTGCA GTGAGAAGAG GTGTCCAGCC TTCCATGAGA TGTGCAGGGA TGAGTACGTG 
ATGTTGATGA CATGGAAGAG GGCGGCAGCT GGCGAGATCA TTTACAACAA GTGTCCCCCT 
AATGCCTCGG GTTCTGCTAG CCGCCGCTGT CTCCTCAGTG CCCAGGGCGT AGCATACTGG 
GGACTGCCCA GCTTTGCTCG TTGCATATCC CATGAATACC GCTACCTGTA CCTGTCACTT 
CGGGAACACC TGGCTAAGGG CCAGCGCATG CTGGCAGGTG AGGGCATGTC ACAGGTGGTG 
CGGAGCCTGC AGGAGCTACT GGCACGGCGC ACTTACTACA GCGGGGACCT GCTCTTCTCT 
GTGGACATCC TAAGGAACGT CACTGACACC TTCAAGAGGG CCACCTATGT CCCTTCCGCC 
GATGACGTGC AGCGTTTCTT CCAGGTGGTG AGCTTCATGG TGGATTCAGA AAACAAGGAC 
AAATGGGATG ATGCTCAGCA GGTGTCACCG GGCTCTGTGC ACCTGCTGCG TGTTGTGGAA 
GATTTCATTC ACCTCGTGGG CGACGCTCTC AAGGCCTTCC AGAGCTCTCT CATTGTCACG 
GACAATCTGG TGATCAGCAT TCAGAGAGAG CCTATCTCCG CCGTGTCCAG TGACATCACG 
TTTCCCATGC GGGGCCGCAG GGGCATGAAG GACTGGGTGC GACACTCAGA GGATCGTCTC 
TTTCTACCCA AGGAGGTGCT CAGCCTGTCC TCCCCAGGAA AGCCAGCCAC ACCTGGGGCA 
GCCACAGCAG GCAGCCCGGG CAGGGGGAGG GGCCCAGGAA CGGTGCCCCC TGGCCCAGGC 
CACGCCCACC AGCGCCTTCT CCCAGCTGAC CCCGAAGAGT CCTCCTCCTA CTTTGTGATC 
GGTGCTGTGC TCTACCGCAC CCTTGGCCTC ATCCTGCCGC CCCCCAGGCC TCCACTTGCT 
GTCACCTCCC GGGTGATGAC AGTGACTGTG CGTCCCCCCA CCCAGCCTCC AGCTGAGCCC 
CTCATTACAG TGGAACTCTC GTACATCATC AATGGCACCA CCGATCCCCA CTGTGCCAGC 
TGGGACTACT CCAGAGCAGA TACCAACTCG GGGGACTGGA ACACTGAGAG CTGCCAGACC 
TTGGAGACCC AGGCGGCTCA CACCCGCTGC CAGTGCCAGC ACCTGTCCAC CTTTGCCGTC 
CTGGCCCAGC CACCCAAGGA CCTGACCCTG GAGCTGGCAG GTGCTCCCTC TGTCCCCCTG 
GTGATCGGCT GTGCAGTGTC CTGCATGGCT CTGCTCACCC TGCTGGCCAT CTATGCAGCC 
TTCTGGAGGT TTATAAAATC AGAACGCTCC ATCATCTTGC TGAACTTCTG CCTGTCCATC 
CTGGCTTCCA ACATTCTGAT CCTGGTGGGC CAGTCCCGGG TGCTGAGCAA GGGCGTATGC 
ACCATGACGG CTGCCTTCCT ACACTTCTTC TTTCTGTCCT CCTTTTGCTG GGTGCTTACA 
GAGGCCTGGC AATCCTATCT GGCTGTCATC GGGCGGATGC GCACCCGCCT GGTTCGCAAG 
CGCTTCCTCT GCCTGGGCTG GGGTCTACCT GCCCTGGTGG TGGCTGTGTC TGTTGGCTTT 
ACTCGCACCA AAGGATATGG TACATCCAGC TACTGCTGGC TGTCCCTAGA GGGCGGCCTG 
CTCTATGCCT TCGTGGGTCC AGCAGCAGTC ATTGTCCTGG TGAACATGCT CATCGGGATT 
ATCGTCTTCA ACAAGCTCAT GGCTCGCGAT GGCGTCTCAG ACAAATCTAA GAAGCAGAGG 
GCTGGGGCTT CACTCTGGAG TTCCTGCGTG GTACTGCCTC TCCTGGCGCT TACCTGGATG 
TCTGCCGTCC TGGCCATGAC AGATCGCCGC TCCGTCCTCT TCCAGGCACT CTTTGCCGTT 
TTCAACTCTG CACAAGGCTT TGTCATCACC GCTGTGCACT GCTTCCTGCG CCGAGAGGTC 
CAGGATGTGG TAAAGTGTCA GATGGGTGTG TGTCGGGCTG ATGAGAGTGA AGACTCCCCA 
GACTCGTGCA AGAACGGGCA GCTGCAGATC CTGTCAGACT TTGAAAAGGA CGTGGATCTG 
GCTTGTCAGA CAGTTCTGTT CAAGGAGGTC AACACCTGCA ACCCGTCCAC CATTACCGGC 
ACTCTGTCCC GCCTGTCTCT GGATGAGGAT GAGGAGCCCA AGTCCTGTCT CGTGGGTCCT 
GAGGGTGGCC TCAGCTTCTC ACCACTGCCT GGGAACATCC TGGTACCCAT GGCAGCCTCA 
CCAGGTCTAG GGGAGCCACC ACCACCCCAG GAGACCAACC CTGTGTACAT GTGTGGGGAG 
GGTGGCCTGC GGCAGTTGGA CCTTACATGG ATACGGCAGA GTGAACCAGG CTCTGAGGGG 
GACTACATGG TTCTGCCCCG GCGGACTTTG AGCCTGCAGC CTGGTGGTGG GGGTACAGCG 
GGTGAGGAGG CCCCAAGGGC CCGGCCTGAG GGGACCCCCC GGCGGGCTGC CAAAACGGTA 
GCCCACACTG AAGGCTACCC CAGCTTCCTG TCTGTGGAGC ACTCGGGTCT AGGGCTGGGC 
CCTGCCTATG GGTCTCTCCA GAACCCGTAT GGAATGACCT TCCAACCACC ACCACCAACA 
CCCAGCGCCC GCCAAGTACC AGAGCCAGGA GAACGTAGCC GGACCATGCC CCGTACAGTG 
CCTGGTTCCA CCATGAAGCT GGGCTCCCTT GAGCGAAAGA AGCTTCGGTA TTCGGACTTG 
GACTTTGAGA AGGTGATGCA CACTCGGAAA CGGCACTCGG AACTCTACCA CGAACTCAAC 
CAGAAGTTCC ACACTTTCGA CCGCTACCGT AGCCAGTCCT CAGCCAAGGA GAAACCCAGC 
CCCCCCGGGG GACGCCCTGG CTTGTCCCAG CACAGGAGGC ATCAAAGCTG GAGCACCTTC 
AAATCTATGA CACTGGGCTC ACTGCCCCCC AAGCCCCGAG AACGGCTGGC CCTGCACCGG 
ACAGCAGCCT GGGAGCCCAC AGAACCGCCA GACGGCGACT TCCAGACAGA GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adgrb2 ( NM_001199696.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001186625.1 Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant 2, mRNA.

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CloneID	OMu14259
Clone ID Related Accession (Same CDS sequence)	NM_001199696.1 , NM_001199696.2
Accession Version	NM_001199696.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4521bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-11
Organism	Mus musculus(house mouse)
Product	adhesion G protein-coupled receptor B2 isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL626774.23. On Dec 12, 2019 this sequence version replaced NM_001199696.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK158735.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849379 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCCCAG CCTGTCCCCT CTTACTGTCT GTGATTCTGT CCCTGCGCCT GGCCACGGCC 
TTCGACCCTG CCCCCAGTGC CTGCTCTGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTC 
TCGCTGCAGG ACCTCTTTCC CACCATCGCC TCGGGCTGCT CCTGGACCCT GGAGAACCCA 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGGC AGGAGCAGGT TTGCACACAC 
TTTGCCCCGC GCCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTTACCTG CCTGCGGCCT 
GGTCCAGAGG AAGCCACAGC CCGGGCTGAG TCGGAGGTGG GACGGCCAGA GGAGGAGGAG 
GAGGAGGCGG CGGCAGCAGC ATCAGGGTTG GAGTTGTGTG GTGGCTCAGG CCCCTTTACC 
TTTCTGCACT TCGACAAGAA CTTTGTGCAG CTGTGCCTGT CGGCTGAGCC CTCTGAGGCC 
CCTCGTCTGC TAGCGCCTGC TGCCCTGGCC TTCCGTTTTG TGGAGGTCTT GCTGATCAAC 
AACAACAACT CCAGCCAGTT CACCTGTGGT GTGCTCTGCC GCTGGAGTGA GGAGTGTGGC 
CGGGCTGCAG GCAGGGCTTG TGGCTTTGCA CAGCCAGGGT GTAGTTGTCC TGGGGAGGCA 
GGGGCCAACC CCGCCACCAC CACATCTCCG GGGCCTCCGG TTGCCCACAC CCTGTCCAAT 
GCCCTGGTGC CCGGGGGCCC AGCCCCTCCT GCTGAGGCCG ACTTGCACTC GGGGAGCAGC 
AATGACCTGT TCACCACCGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG 
AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTAT ACATGGCGCA GACAGTGCAC 
GGCGTGTGGG AGGAGTGGGG GTCCTGGAGC CTGTGCTCCC GCAGCTGCGG GCGGGGGTCT 
CGGAGCCGGA TGCGGACCTG CGTGCCCCCC CAGCACGGCG GCAAGGCCTG CGAGGGTCCC 
GAGCTGCAGA CTAAACTCTG CAGTATGGCC GCCTGCCCGG TGGAAGGCCA GTGGCTAGAA 
TGGGGTCCCT GGGGCCCATG CTCATCATCT TGTGCCAATG GGACCCAGCA GCGCAGCCGG 
AAATGCAGTG TGGCGGGTCC AGCCTGGGCC ACGTGCGCAG GTGCCCTCAC GGATACCCGT 
GAGTGCAGCA ATCTCGATTG CCCGGCCACT GACGGCAAGT GGGGGCCGTG GAACGCGTGG 
AGCCTGTGCT CCAAGACGTG TGACACGGGC TGGCAACGCC GCTTCCGCAT GTGCCAGGCT 
TCTGGCACAC AGGGCTACCC TTGCGAGGGC ACAGGAGAGG AGGTGAAACC CTGCAGTGAG 
AAGAGGTGTC CAGCCTTCCA TGAGATGTGC AGGGATGAGT ACGTGATGTT GATGACATGG 
AAGAGGGCGG CAGCTGGCGA GATCATTTAC AACAAGTGTC CCCCTAATGC CTCGGGTTCT 
GCTAGCCGCC GCTGTCTCCT CAGTGCCCAG GGCGTAGCAT ACTGGGGACT GCCCAGCTTT 
GCTCGTTGCA TATCCCATGA ATACCGCTAC CTGTACCTGT CACTTCGGGA ACACCTGGCT 
AAGGGCCAGC GCATGCTGGC AGGTGAGGGC ATGTCACAGG TGGTGCGGAG CCTGCAGGAG 
CTACTGGCAC GGCGCACTTA CTACAGCGGG GACCTGCTCT TCTCTGTGGA CATCCTAAGG 
AACGTCACTG ACACCTTCAA GAGGGCCACC TATGTCCCTT CCGCCGATGA CGTGCAGCGT 
TTCTTCCAGG TGGTGAGCTT CATGGTGGAT TCAGAAAACA AGGACAAATG GGATGATGCT 
CAGCAGGTGT CACCGGGCTC TGTGCACCTG CTGCGTGTTG TGGAAGATTT CATTCACCTC 
GTGGGCGACG CTCTCAAGGC CTTCCAGAGC TCTCTCATTG TCACGGACAA TCTGGTGATC 
AGCATTCAGA GAGAGCCTAT CTCCGCCGTG TCCAGTGACA TCACGTTTCC CATGCGGGGC 
CGCAGGGGCA TGAAGGACTG GGTGCGACAC TCAGAGGATC GTCTCTTTCT ACCCAAGGAG 
GTGCTCAGCC TGTCCTCCCC AGGAAAGCCA GCCACACCTG GGGCAGCCAC AGCAGGCAGC 
CCGGGCAGGG GGAGGGGCCC AGGAACGGTG CCCCCTGGCC CAGGCCACGC CCACCAGCGC 
CTTCTCCCAG CTGACCCCGA AGAGTCCTCC TCCTACTTTG TGATCGGTGC TGTGCTCTAC 
CGCACCCTTG GCCTCATCCT GCCGCCCCCC AGGCCTCCAC TTGCTGTCAC CTCCCGGGTG 
ATGACAGTGA CTGTGCGTCC CCCCACCCAG CCTCCAGCTG AGCCCCTCAT TACAGTGGAA 
CTCTCGTACA TCATCAATGG CACCACCGAT CCCCACTGTG CCAGCTGGGA CTACTCCAGA 
GCAGATACCA ACTCGGGGGA CTGGAACACT GAGAGCTGCC AGACCTTGGA GACCCAGGCG 
GCTCACACCC GCTGCCAGTG CCAGCACCTG TCCACCTTTG CCGTCCTGGC CCAGCCACCC 
AAGGACCTGA CCCTGGAGCT GGCAGGTGCT CCCTCTGTCC CCCTGGTGAT CGGCTGTGCA 
GTGTCCTGCA TGGCTCTGCT CACCCTGCTG GCCATCTATG CAGCCTTCTG GAGGTTTATA 
AAATCAGAAC GCTCCATCAT CTTGCTGAAC TTCTGCCTGT CCATCCTGGC TTCCAACATT 
CTGATCCTGG TGGGCCAGTC CCGGGTGCTG AGCAAGGGCG TATGCACCAT GACGGCTGCC 
TTCCTACACT TCTTCTTTCT GTCCTCCTTT TGCTGGGTGC TTACAGAGGC CTGGCAATCC 
TATCTGGCTG TCATCGGGCG GATGCGCACC CGCCTGGTTC GCAAGCGCTT CCTCTGCCTG 
GGCTGGGGTC TACCTGCCCT GGTGGTGGCT GTGTCTGTTG GCTTTACTCG CACCAAAGGA 
TATGGTACAT CCAGCTACTG CTGGCTGTCC CTAGAGGGCG GCCTGCTCTA TGCCTTCGTG 
GGTCCAGCAG CAGTCATTGT CCTGGTGAAC ATGCTCATCG GGATTATCGT CTTCAACAAG 
CTCATGGCTC GCGATGGCGT CTCAGACAAA TCTAAGAAGC AGAGGGCTGG GTCGGAGCGG 
TGCCCCTGGG CCAGCCTGCT CCTTCCCTGC TCAGCGTGTG GAGCGGTCCC CAGCCCCCTG 
CTCAGCTCAG CCTCGGCCAG GAATGCCATG GCTTCACTCT GGAGTTCCTG CGTGGTACTG 
CCTCTCCTGG CGCTTACCTG GATGTCTGCC GTCCTGGCCA TGACAGATCG CCGCTCCGTC 
CTCTTCCAGG CACTCTTTGC CGTTTTCAAC TCTGCACAAG GCTTTGTCAT CACCGCTGTG 
CACTGCTTCC TGCGCCGAGA GGTCCAGGAT GTGGTAAAGT GTCAGATGGG TGTGTGTCGG 
GCTGATGAGA GTGAAGACTC CCCAGACTCG TGCAAGAACG GGCAGCTGCA GATCCTGTCA 
GACTTTGAAA AGGACGTGGA TCTGGCTTGT CAGACAGTTC TGTTCAAGGA GGTCAACACC 
TGCAACCCGT CCACCATTAC CGGCACTCTG TCCCGCCTGT CTCTGGATGA GGATGAGGAG 
CCCAAGTCCT GTCTCGTGGG TCCTGAGGGT GGCCTCAGCT TCTCACCACT GCCTGGGAAC 
ATCCTGGTAC CCATGGCAGC CTCACCAGGT CTAGGGGAGC CACCACCACC CCAGGAGACC 
AACCCTGTGT ACATGTGTGG GGAGGGTGGC CTGCGGCAGT TGGACCTTAC ATGGATACGG 
CAGAGTGAAC CAGGCTCTGA GGGGGACTAC ATGGTTCTGC CCCGGCGGAC TTTGAGCCTG 
CAGCCTGGTG GTGGGGGTAC AGCGGGTGAG GAGGCCCCAA GGGCCCGGCC TGAGGGGACC 
CCCCGGCGGG CTGCCAAAAC GGTAGCCCAC ACTGAAGGCT ACCCCAGCTT CCTGTCTGTG 
GAGCACTCGG GTCTAGGGCT GGGCCCTGCC TATGGGTCTC TCCAGAACCC GTATGGAATG 
ACCTTCCAAC CACCACCACC AACACCCAGC GCCCGCCAAG TACCAGAGCC AGGAGAACGT 
AGCCGGACCA TGCCCCGTAC AGTGCCTGGT TCCACCATGA AGCTGGGCTC CCTTGAGCGA 
AAGAAGCTTC GGTATTCGGA CTTGGACTTT GAGAAGGTGA TGCACACTCG GAAACGGCAC 
TCGGAACTCT ACCACGAACT CAACCAGAAG TTCCACACTT TCGACCGCTA CCGTAGCCAG 
TCCTCAGCCA AGGAGAAACC CAGCCCCCCC GGGGGACGCC CTGGCTTGTC CCAGCACAGG 
AGGCATCAAA GCTGGAGCAC CTTCAAATCT ATGACACTGG GCTCACTGCC CCCCAAGCCC 
CGAGAACGGC TGGCCCTGCA CCGGACAGCA GCCTGGGAGC CCACAGAACC GCCAGACGGC 
GACTTCCAGA CAGAGGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186625.1
CDS	454..4974
Translation	MTPACPLLLSVILSLRLATAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCTHFAPRLLPLDHYLVNFTCLRPGPEEATARAESEVGRPEEEEEEAAAAASGLELCGGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGANPATTTSPGPPVAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSSSCANGTQQRSRKCSVAGPAWATCAGALTDTRECSNLDCPATDGKWGPWNAWSLCSKTCDTGWQRRFRMCQASGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKRAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDSENKDKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPISAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSSPGKPATPGAATAGSPGRGRGPGTVPPGPGHAHQRLLPADPEESSSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADTNSGDWNTESCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGAPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNILILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGVSDKSKKQRAGSERCPWASLLLPCSACGAVPSPLLSSASARNAMASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGGLSFSPLPGNILVPMAASPGLGEPPPPQETNPVYMCGEGGLRQLDLTWIRQSEPGSEGDYMVLPRRTLSLQPGGGGTAGEEAPRARPEGTPRRAAKTVAHTEGYPSFLSVEHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVPEPGERSRTMPRTVPGSTMKLGSLERKKLRYSDLDFEKVMHTRKRHSELYHELNQKFHTFDRYRSQSSAKEKPSPPGGRPGLSQHRRHQSWSTFKSMTLGSLPPKPRERLALHRTAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	NM_001199696.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199696.2

ORF Insert Sequence:

ATGACCCCAG CCTGTCCCCT CTTACTGTCT GTGATTCTGT CCCTGCGCCT GGCCACGGCC 
TTCGACCCTG CCCCCAGTGC CTGCTCTGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTC 
TCGCTGCAGG ACCTCTTTCC CACCATCGCC TCGGGCTGCT CCTGGACCCT GGAGAACCCA 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGGC AGGAGCAGGT TTGCACACAC 
TTTGCCCCGC GCCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTTACCTG CCTGCGGCCT 
GGTCCAGAGG AAGCCACAGC CCGGGCTGAG TCGGAGGTGG GACGGCCAGA GGAGGAGGAG 
GAGGAGGCGG CGGCAGCAGC ATCAGGGTTG GAGTTGTGTG GTGGCTCAGG CCCCTTTACC 
TTTCTGCACT TCGACAAGAA CTTTGTGCAG CTGTGCCTGT CGGCTGAGCC CTCTGAGGCC 
CCTCGTCTGC TAGCGCCTGC TGCCCTGGCC TTCCGTTTTG TGGAGGTCTT GCTGATCAAC 
AACAACAACT CCAGCCAGTT CACCTGTGGT GTGCTCTGCC GCTGGAGTGA GGAGTGTGGC 
CGGGCTGCAG GCAGGGCTTG TGGCTTTGCA CAGCCAGGGT GTAGTTGTCC TGGGGAGGCA 
GGGGCCAACC CCGCCACCAC CACATCTCCG GGGCCTCCGG TTGCCCACAC CCTGTCCAAT 
GCCCTGGTGC CCGGGGGCCC AGCCCCTCCT GCTGAGGCCG ACTTGCACTC GGGGAGCAGC 
AATGACCTGT TCACCACCGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG 
AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTAT ACATGGCGCA GACAGTGCAC 
GGCGTGTGGG AGGAGTGGGG GTCCTGGAGC CTGTGCTCCC GCAGCTGCGG GCGGGGGTCT 
CGGAGCCGGA TGCGGACCTG CGTGCCCCCC CAGCACGGCG GCAAGGCCTG CGAGGGTCCC 
GAGCTGCAGA CTAAACTCTG CAGTATGGCC GCCTGCCCGG TGGAAGGCCA GTGGCTAGAA 
TGGGGTCCCT GGGGCCCATG CTCATCATCT TGTGCCAATG GGACCCAGCA GCGCAGCCGG 
AAATGCAGTG TGGCGGGTCC AGCCTGGGCC ACGTGCGCAG GTGCCCTCAC GGATACCCGT 
GAGTGCAGCA ATCTCGATTG CCCGGCCACT GACGGCAAGT GGGGGCCGTG GAACGCGTGG 
AGCCTGTGCT CCAAGACGTG TGACACGGGC TGGCAACGCC GCTTCCGCAT GTGCCAGGCT 
TCTGGCACAC AGGGCTACCC TTGCGAGGGC ACAGGAGAGG AGGTGAAACC CTGCAGTGAG 
AAGAGGTGTC CAGCCTTCCA TGAGATGTGC AGGGATGAGT ACGTGATGTT GATGACATGG 
AAGAGGGCGG CAGCTGGCGA GATCATTTAC AACAAGTGTC CCCCTAATGC CTCGGGTTCT 
GCTAGCCGCC GCTGTCTCCT CAGTGCCCAG GGCGTAGCAT ACTGGGGACT GCCCAGCTTT 
GCTCGTTGCA TATCCCATGA ATACCGCTAC CTGTACCTGT CACTTCGGGA ACACCTGGCT 
AAGGGCCAGC GCATGCTGGC AGGTGAGGGC ATGTCACAGG TGGTGCGGAG CCTGCAGGAG 
CTACTGGCAC GGCGCACTTA CTACAGCGGG GACCTGCTCT TCTCTGTGGA CATCCTAAGG 
AACGTCACTG ACACCTTCAA GAGGGCCACC TATGTCCCTT CCGCCGATGA CGTGCAGCGT 
TTCTTCCAGG TGGTGAGCTT CATGGTGGAT TCAGAAAACA AGGACAAATG GGATGATGCT 
CAGCAGGTGT CACCGGGCTC TGTGCACCTG CTGCGTGTTG TGGAAGATTT CATTCACCTC 
GTGGGCGACG CTCTCAAGGC CTTCCAGAGC TCTCTCATTG TCACGGACAA TCTGGTGATC 
AGCATTCAGA GAGAGCCTAT CTCCGCCGTG TCCAGTGACA TCACGTTTCC CATGCGGGGC 
CGCAGGGGCA TGAAGGACTG GGTGCGACAC TCAGAGGATC GTCTCTTTCT ACCCAAGGAG 
GTGCTCAGCC TGTCCTCCCC AGGAAAGCCA GCCACACCTG GGGCAGCCAC AGCAGGCAGC 
CCGGGCAGGG GGAGGGGCCC AGGAACGGTG CCCCCTGGCC CAGGCCACGC CCACCAGCGC 
CTTCTCCCAG CTGACCCCGA AGAGTCCTCC TCCTACTTTG TGATCGGTGC TGTGCTCTAC 
CGCACCCTTG GCCTCATCCT GCCGCCCCCC AGGCCTCCAC TTGCTGTCAC CTCCCGGGTG 
ATGACAGTGA CTGTGCGTCC CCCCACCCAG CCTCCAGCTG AGCCCCTCAT TACAGTGGAA 
CTCTCGTACA TCATCAATGG CACCACCGAT CCCCACTGTG CCAGCTGGGA CTACTCCAGA 
GCAGATACCA ACTCGGGGGA CTGGAACACT GAGAGCTGCC AGACCTTGGA GACCCAGGCG 
GCTCACACCC GCTGCCAGTG CCAGCACCTG TCCACCTTTG CCGTCCTGGC CCAGCCACCC 
AAGGACCTGA CCCTGGAGCT GGCAGGTGCT CCCTCTGTCC CCCTGGTGAT CGGCTGTGCA 
GTGTCCTGCA TGGCTCTGCT CACCCTGCTG GCCATCTATG CAGCCTTCTG GAGGTTTATA 
AAATCAGAAC GCTCCATCAT CTTGCTGAAC TTCTGCCTGT CCATCCTGGC TTCCAACATT 
CTGATCCTGG TGGGCCAGTC CCGGGTGCTG AGCAAGGGCG TATGCACCAT GACGGCTGCC 
TTCCTACACT TCTTCTTTCT GTCCTCCTTT TGCTGGGTGC TTACAGAGGC CTGGCAATCC 
TATCTGGCTG TCATCGGGCG GATGCGCACC CGCCTGGTTC GCAAGCGCTT CCTCTGCCTG 
GGCTGGGGTC TACCTGCCCT GGTGGTGGCT GTGTCTGTTG GCTTTACTCG CACCAAAGGA 
TATGGTACAT CCAGCTACTG CTGGCTGTCC CTAGAGGGCG GCCTGCTCTA TGCCTTCGTG 
GGTCCAGCAG CAGTCATTGT CCTGGTGAAC ATGCTCATCG GGATTATCGT CTTCAACAAG 
CTCATGGCTC GCGATGGCGT CTCAGACAAA TCTAAGAAGC AGAGGGCTGG GTCGGAGCGG 
TGCCCCTGGG CCAGCCTGCT CCTTCCCTGC TCAGCGTGTG GAGCGGTCCC CAGCCCCCTG 
CTCAGCTCAG CCTCGGCCAG GAATGCCATG GCTTCACTCT GGAGTTCCTG CGTGGTACTG 
CCTCTCCTGG CGCTTACCTG GATGTCTGCC GTCCTGGCCA TGACAGATCG CCGCTCCGTC 
CTCTTCCAGG CACTCTTTGC CGTTTTCAAC TCTGCACAAG GCTTTGTCAT CACCGCTGTG 
CACTGCTTCC TGCGCCGAGA GGTCCAGGAT GTGGTAAAGT GTCAGATGGG TGTGTGTCGG 
GCTGATGAGA GTGAAGACTC CCCAGACTCG TGCAAGAACG GGCAGCTGCA GATCCTGTCA 
GACTTTGAAA AGGACGTGGA TCTGGCTTGT CAGACAGTTC TGTTCAAGGA GGTCAACACC 
TGCAACCCGT CCACCATTAC CGGCACTCTG TCCCGCCTGT CTCTGGATGA GGATGAGGAG 
CCCAAGTCCT GTCTCGTGGG TCCTGAGGGT GGCCTCAGCT TCTCACCACT GCCTGGGAAC 
ATCCTGGTAC CCATGGCAGC CTCACCAGGT CTAGGGGAGC CACCACCACC CCAGGAGACC 
AACCCTGTGT ACATGTGTGG GGAGGGTGGC CTGCGGCAGT TGGACCTTAC ATGGATACGG 
CAGAGTGAAC CAGGCTCTGA GGGGGACTAC ATGGTTCTGC CCCGGCGGAC TTTGAGCCTG 
CAGCCTGGTG GTGGGGGTAC AGCGGGTGAG GAGGCCCCAA GGGCCCGGCC TGAGGGGACC 
CCCCGGCGGG CTGCCAAAAC GGTAGCCCAC ACTGAAGGCT ACCCCAGCTT CCTGTCTGTG 
GAGCACTCGG GTCTAGGGCT GGGCCCTGCC TATGGGTCTC TCCAGAACCC GTATGGAATG 
ACCTTCCAAC CACCACCACC AACACCCAGC GCCCGCCAAG TACCAGAGCC AGGAGAACGT 
AGCCGGACCA TGCCCCGTAC AGTGCCTGGT TCCACCATGA AGCTGGGCTC CCTTGAGCGA 
AAGAAGCTTC GGTATTCGGA CTTGGACTTT GAGAAGGTGA TGCACACTCG GAAACGGCAC 
TCGGAACTCT ACCACGAACT CAACCAGAAG TTCCACACTT TCGACCGCTA CCGTAGCCAG 
TCCTCAGCCA AGGAGAAACC CAGCCCCCCC GGGGGACGCC CTGGCTTGTC CCAGCACAGG 
AGGCATCAAA GCTGGAGCAC CTTCAAATCT ATGACACTGG GCTCACTGCC CCCCAAGCCC 
CGAGAACGGC TGGCCCTGCA CCGGACAGCA GCCTGGGAGC CCACAGAACC GCCAGACGGC 
GACTTCCAGA CAGAGGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adgrb2 ( NM_001290715.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001277644.1 Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu14294
Clone ID Related Accession (Same CDS sequence)	NM_001290715.1 , NM_001290715.2
Accession Version	NM_001290715.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-11
Organism	Mus musculus(house mouse)
Product	adhesion G protein-coupled receptor B2 isoform 3 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL626774.23. On Dec 12, 2019 this sequence version replaced NM_001290715.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AY168407.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849379 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGACCCCAG CCTGTCCCCT CTTACTGTCT GTGATTCTGT CCCTGCGCCT GGCCACGGCC 
TTCGACCCTG CCCCCAGTGC CTGCTCTGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTC 
TCGCTGCAGG ACCTCTTTCC CACCATCGCC TCGGGCTGCT CCTGGACCCT GGAGAACCCA 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGGC AGGAGCAGGT TTGCACACAC 
TTTGCCCCGC GCCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTTACCTG CCTGCGGCCT 
GGTCCAGAGG AAGCCACAGC CCGGGCTGAG TCGGAGGTGG GACGGCCAGA GGAGGAGGAG 
GAGGAGGCGG CGGCAGCAGC ATCAGGGTTG GAGTTGTGTG GTGGCTCAGG CCCCTTTACC 
TTTCTGCACT TCGACAAGAA CTTTGTGCAG CTGTGCCTGT CGGCTGAGCC CTCTGAGGCC 
CCTCGTCTGC TAGCGCCTGC TGCCCTGGCC TTCCGTTTTG TGGAGGTCTT GCTGATCAAC 
AACAACAACT CCAGCCAGTT CACCTGTGGT GTGCTCTGCC GCTGGAGTGA GGAGTGTGGC 
CGGGCTGCAG GCAGGGCTTG TGGCTTTGCA CAGCCAGGGT GTAGTTGTCC TGGGGAGGCA 
GGGGCCAACC CCGCCACCAC CACATCTCCG GGGCCTCCGG TTGCCCACAC CCTGTCCAAT 
GCCCTGGTGC CCGGGGGCCC AGCCCCTCCT GCTGAGGCCG ACTTGCACTC GGGGAGCAGC 
AATGACCTGT TCACCACCGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG 
AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTAT ACATGGCGCA GACAGGCGAC 
CCAGCAGCTG AGGAGTGGTC CCCGTGGAGC GTGTGTTCCC TGACGTGTGG GCAGGGTCTG 
CAGGTGCGGA CCCGCTCCTG CGTGTCCTCC CCCTATGGGA CCCTGTGCAG CGGGCCCCTT 
CGGGAGACCC GGCCTTGCAA CAATTCAGCC ACCTGCCCAG TGCACGGCGT GTGGGAGGAG 
TGGGGGTCCT GGAGCCTGTG CTCCCGCAGC TGCGGGCGGG GGTCTCGGAG CCGGATGCGG 
ACCTGCGTGC CCCCCCAGCA CGGCGGCAAG GCCTGCGAGG GTCCCGAGCT GCAGACTAAA 
CTCTGCAGTA TGGCCGCCTG CCCGGTGGAA GGCCAGTGGC TAGAATGGGG TCCCTGGGGC 
CCATGCTCAT CATCTTGTGC CAATGGGACC CAGCAGCGCA GCCGGAAATG CAGTGTGGCG 
GGTCCAGCCT GGGCCACGTG CGCAGGTGCC CTCACGGATA CCCGTGAGTG CAGCAATCTC 
GATTGCCCGG CCACTGACGG CAAGTGGGGG CCGTGGAACG CGTGGAGCCT GTGCTCCAAG 
ACGTGTGACA CGGGCTGGCA ACGCCGCTTC CGCATGTGCC AGGCTTCTGG CACACAGGGC 
TACCCTTGCG AGGGCACAGG AGAGGAGGTG AAACCCTGCA GTGAGAAGAG GTGTCCAGCC 
TTCCATGAGA TGTGCAGGGA TGAGTACGTG ATGTTGATGA CATGGAAGAG GGCGGCAGCT 
GGCGAGATCA TTTACAACAA GTGTCCCCCT AATGCCTCGG GTTCTGCTAG CCGCCGCTGT 
CTCCTCAGTG CCCAGGGCGT AGCATACTGG GGACTGCCCA GCTTTGCTCG TTGCATATCC 
CATGAATACC GCTACCTGTA CCTGTCACTT CGGGAACACC TGGCTAAGGG CCAGCGCATG 
CTGGCAGGTG AGGGCATGTC ACAGGTGGTG CGGAGCCTGC AGGAGCTACT GGCACGGCGC 
ACTTACTACA GCGGGGACCT GCTCTTCTCT GTGGACATCC TAAGGAACGT CACTGACACC 
TTCAAGAGGG CCACCTATGT CCCTTCCGCC GATGACGTGC AGCGTTTCTT CCAGGTGGTG 
AGCTTCATGG TGGATTCAGA AAACAAGGAC AAATGGGATG ATGCTCAGCA GGTGTCACCG 
GGCTCTGTGC ACCTGCTGCG TGTTGTGGAA GATTTCATTC ACCTCGTGGG CGACGCTCTC 
AAGGCCTTCC AGAGCTCTCT CATTGTCACG GACAATCTGG TGATCAGCAT TCAGAGAGAG 
CCTATCTCCG CCGTGTCCAG TGACATCACG TTTCCCATGC GGGGCCGCAG GGGCATGAAG 
GACTGGGTGC GACACTCAGA GGATCGTCTC TTTCTACCCA AGGAGGTGCT CAGCCTGTCC 
TCCCCAGGAA AGCCAGCCAC ACCTGGGGCA GCCACAGCAG GCAGCCCGGG CAGGGGGAGG 
GGCCCAGGAA CGGTGCCCCC TGGCCCAGGC CACGCCCACC AGCGCCTTCT CCCAGCTGAC 
CCCGAAGAGT CCTCCTCCTA CTTTGTGATC GGTGCTGTGC TCTACCGCAC CCTTGGCCTC 
ATCCTGCCGC CCCCCAGGCC TCCACTTGCT GTCACCTCCC GGGTGATGAC AGTGACTGTG 
CGTCCCCCCA CCCAGCCTCC AGCTGAGCCC CTCATTACAG TGGAACTCTC GTACATCATC 
AATGGCACCA CCGATCCCCA CTGTGCCAGC TGGGACTACT CCAGAGCAGA TACCAACTCG 
GGGGACTGGA ACACTGAGAG CTGCCAGACC TTGGAGACCC AGGCGGCTCA CACCCGCTGC 
CAGTGCCAGC ACCTGTCCAC CTTTGCCGTC CTGGCCCAGC CACCCAAGGA CCTGACCCTG 
GAGCTGGCAG GTGCTCCCTC TGTCCCCCTG GTGATCGGCT GTGCAGTGTC CTGCATGGCT 
CTGCTCACCC TGCTGGCCAT CTATGCAGCC TTCTGGAGGT TTATAAAATC AGAACGCTCC 
ATCATCTTGC TGAACTTCTG CCTGTCCATC CTGGCTTCCA ACATTCTGAT CCTGGTGGGC 
CAGTCCCGGG TGCTGAGCAA GGGCGTATGC ACCATGACGG CTGCCTTCCT ACACTTCTTC 
TTTCTGTCCT CCTTTTGCTG GGTGCTTACA GAGGCCTGGC AATCCTATCT GGCTGTCATC 
GGGCGGATGC GCACCCGCCT GGTTCGCAAG CGCTTCCTCT GCCTGGGCTG GGGTCTACCT 
GCCCTGGTGG TGGCTGTGTC TGTTGGCTTT ACTCGCACCA AAGGATATGG TACATCCAGC 
TACTGCTGGC TGTCCCTAGA GGGCGGCCTG CTCTATGCCT TCGTGGGTCC AGCAGCAGTC 
ATTGTCCTGG TGAACATGCT CATCGGGATT ATCGTCTTCA ACAAGCTCAT GGCTCGCGAT 
GGCGTCTCAG ACAAATCTAA GAAGCAGAGG GCTGGGTCGG AGCGGTGCCC CTGGGCCAGC 
CTGCTCCTTC CCTGCTCAGC GTGTGGAGCG GTCCCCAGCC CCCTGCTCAG CTCAGCCTCG 
GCCAGGAATG CCATGGCTTC ACTCTGGAGT TCCTGCGTGG TACTGCCTCT CCTGGCGCTT 
ACCTGGATGT CTGCCGTCCT GGCCATGACA GATCGCCGCT CCGTCCTCTT CCAGGCACTC 
TTTGCCGTTT TCAACTCTGC ACAAGGCTTT GTCATCACCG CTGTGCACTG CTTCCTGCGC 
CGAGAGGTCC AGGATGTGGT AAAGTGTCAG ATGGGTGTGT GTCGGGCTGA TGAGAGTGAA 
GACTCCCCAG ACTCGTGCAA GAACGGGCAG CTGCAGATCC TGTCAGACTT TGAAAAGGAC 
GTGGATCTGG CTTGTCAGAC AGTTCTGTTC AAGGAGGTCA ACACCTGCAA CCCGTCCACC 
ATTACCGGCA CTCTGTCCCG CCTGTCTCTG GATGAGGATG AGGAGCCCAA GTCCTGTCTC 
GTGGGTCCTG AGGGTGGCCT CAGCTTCTCA CCACTGCCTG GGAACATCCT GGTACCCATG 
GCAGCCTCAC CAGGTCTAGG GGAGCCACCA CCACCCCAGG AGACCAACCC TGTGTACATG 
TGTGGGGAGG GTGGCCTGCG GCAGTTGGAC CTTACATGGA TACGGCAGAG TGAACCAGGC 
TCTGAGGGGG ACTACATGGT TCTGCCCCGG CGGACTTTGA GCCTGCAGCC TGGTGGTGGG 
GGTACAGCGG GTGAGGAGGC CCCAAGGGCC CGGCCTGAGG GGACCCCCCG GCGGGCTGCC 
AAAACGGTAG CCCACACTGA AGGCTACCCC AGCTTCCTGT CTGTGGAGCA CTCGGGTCTA 
GGGCTGGGCC CTGCCTATGG GTCTCTCCAG AACCCGTATG GAATGACCTT CCAACCACCA 
CCACCAACAC CCAGCGCCCG CCAAGTACCA GAGCCAGGAG AACGTAGCCG GACCATGCCC 
CGTACAGTGC CTGGTTCCAC CATGAAGCTG GGCTCCCTTG AGCGAAAGAA GCTTCGGTAT 
TCGGACTTGG ACTTTGAGAA GGTGATGCAC ACTCGGAAAC GGCACTCGGA ACTCTACCAC 
GAACTCAACC AGAAGTTCCA CACTTTCGAC CGCTACCGTA GCCAGTCCTC AGCCAAGGAG 
AAACCCAGCC CCCCCGGGGG ACGCCCTGGC TTGTCCCAGC ACAGGAGGCA TCAAAGCTGG 
AGCACCTTCA AATCTATGAC ACTGGGCTCA CTGCCCCCCA AGCCCCGAGA ACGGCTGGCC 
CTGCACCGGA CAGCAGCCTG GGAGCCCACA GAACCGCCAG ACGGCGACTT CCAGACAGAG 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001277644.1
CDS	454..5139
Translation	MTPACPLLLSVILSLRLATAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCTHFAPRLLPLDHYLVNFTCLRPGPEEATARAESEVGRPEEEEEEAAAAASGLELCGGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGANPATTTSPGPPVAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSSSCANGTQQRSRKCSVAGPAWATCAGALTDTRECSNLDCPATDGKWGPWNAWSLCSKTCDTGWQRRFRMCQASGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKRAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDSENKDKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPISAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSSPGKPATPGAATAGSPGRGRGPGTVPPGPGHAHQRLLPADPEESSSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADTNSGDWNTESCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGAPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNILILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGVSDKSKKQRAGSERCPWASLLLPCSACGAVPSPLLSSASARNAMASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGGLSFSPLPGNILVPMAASPGLGEPPPPQETNPVYMCGEGGLRQLDLTWIRQSEPGSEGDYMVLPRRTLSLQPGGGGTAGEEAPRARPEGTPRRAAKTVAHTEGYPSFLSVEHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVPEPGERSRTMPRTVPGSTMKLGSLERKKLRYSDLDFEKVMHTRKRHSELYHELNQKFHTFDRYRSQSSAKEKPSPPGGRPGLSQHRRHQSWSTFKSMTLGSLPPKPRERLALHRTAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	NM_001290715.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001290715.2

ORF Insert Sequence:

ATGACCCCAG CCTGTCCCCT CTTACTGTCT GTGATTCTGT CCCTGCGCCT GGCCACGGCC 
TTCGACCCTG CCCCCAGTGC CTGCTCTGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTC 
TCGCTGCAGG ACCTCTTTCC CACCATCGCC TCGGGCTGCT CCTGGACCCT GGAGAACCCA 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGGC AGGAGCAGGT TTGCACACAC 
TTTGCCCCGC GCCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTTACCTG CCTGCGGCCT 
GGTCCAGAGG AAGCCACAGC CCGGGCTGAG TCGGAGGTGG GACGGCCAGA GGAGGAGGAG 
GAGGAGGCGG CGGCAGCAGC ATCAGGGTTG GAGTTGTGTG GTGGCTCAGG CCCCTTTACC 
TTTCTGCACT TCGACAAGAA CTTTGTGCAG CTGTGCCTGT CGGCTGAGCC CTCTGAGGCC 
CCTCGTCTGC TAGCGCCTGC TGCCCTGGCC TTCCGTTTTG TGGAGGTCTT GCTGATCAAC 
AACAACAACT CCAGCCAGTT CACCTGTGGT GTGCTCTGCC GCTGGAGTGA GGAGTGTGGC 
CGGGCTGCAG GCAGGGCTTG TGGCTTTGCA CAGCCAGGGT GTAGTTGTCC TGGGGAGGCA 
GGGGCCAACC CCGCCACCAC CACATCTCCG GGGCCTCCGG TTGCCCACAC CCTGTCCAAT 
GCCCTGGTGC CCGGGGGCCC AGCCCCTCCT GCTGAGGCCG ACTTGCACTC GGGGAGCAGC 
AATGACCTGT TCACCACCGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG 
AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTAT ACATGGCGCA GACAGGCGAC 
CCAGCAGCTG AGGAGTGGTC CCCGTGGAGC GTGTGTTCCC TGACGTGTGG GCAGGGTCTG 
CAGGTGCGGA CCCGCTCCTG CGTGTCCTCC CCCTATGGGA CCCTGTGCAG CGGGCCCCTT 
CGGGAGACCC GGCCTTGCAA CAATTCAGCC ACCTGCCCAG TGCACGGCGT GTGGGAGGAG 
TGGGGGTCCT GGAGCCTGTG CTCCCGCAGC TGCGGGCGGG GGTCTCGGAG CCGGATGCGG 
ACCTGCGTGC CCCCCCAGCA CGGCGGCAAG GCCTGCGAGG GTCCCGAGCT GCAGACTAAA 
CTCTGCAGTA TGGCCGCCTG CCCGGTGGAA GGCCAGTGGC TAGAATGGGG TCCCTGGGGC 
CCATGCTCAT CATCTTGTGC CAATGGGACC CAGCAGCGCA GCCGGAAATG CAGTGTGGCG 
GGTCCAGCCT GGGCCACGTG CGCAGGTGCC CTCACGGATA CCCGTGAGTG CAGCAATCTC 
GATTGCCCGG CCACTGACGG CAAGTGGGGG CCGTGGAACG CGTGGAGCCT GTGCTCCAAG 
ACGTGTGACA CGGGCTGGCA ACGCCGCTTC CGCATGTGCC AGGCTTCTGG CACACAGGGC 
TACCCTTGCG AGGGCACAGG AGAGGAGGTG AAACCCTGCA GTGAGAAGAG GTGTCCAGCC 
TTCCATGAGA TGTGCAGGGA TGAGTACGTG ATGTTGATGA CATGGAAGAG GGCGGCAGCT 
GGCGAGATCA TTTACAACAA GTGTCCCCCT AATGCCTCGG GTTCTGCTAG CCGCCGCTGT 
CTCCTCAGTG CCCAGGGCGT AGCATACTGG GGACTGCCCA GCTTTGCTCG TTGCATATCC 
CATGAATACC GCTACCTGTA CCTGTCACTT CGGGAACACC TGGCTAAGGG CCAGCGCATG 
CTGGCAGGTG AGGGCATGTC ACAGGTGGTG CGGAGCCTGC AGGAGCTACT GGCACGGCGC 
ACTTACTACA GCGGGGACCT GCTCTTCTCT GTGGACATCC TAAGGAACGT CACTGACACC 
TTCAAGAGGG CCACCTATGT CCCTTCCGCC GATGACGTGC AGCGTTTCTT CCAGGTGGTG 
AGCTTCATGG TGGATTCAGA AAACAAGGAC AAATGGGATG ATGCTCAGCA GGTGTCACCG 
GGCTCTGTGC ACCTGCTGCG TGTTGTGGAA GATTTCATTC ACCTCGTGGG CGACGCTCTC 
AAGGCCTTCC AGAGCTCTCT CATTGTCACG GACAATCTGG TGATCAGCAT TCAGAGAGAG 
CCTATCTCCG CCGTGTCCAG TGACATCACG TTTCCCATGC GGGGCCGCAG GGGCATGAAG 
GACTGGGTGC GACACTCAGA GGATCGTCTC TTTCTACCCA AGGAGGTGCT CAGCCTGTCC 
TCCCCAGGAA AGCCAGCCAC ACCTGGGGCA GCCACAGCAG GCAGCCCGGG CAGGGGGAGG 
GGCCCAGGAA CGGTGCCCCC TGGCCCAGGC CACGCCCACC AGCGCCTTCT CCCAGCTGAC 
CCCGAAGAGT CCTCCTCCTA CTTTGTGATC GGTGCTGTGC TCTACCGCAC CCTTGGCCTC 
ATCCTGCCGC CCCCCAGGCC TCCACTTGCT GTCACCTCCC GGGTGATGAC AGTGACTGTG 
CGTCCCCCCA CCCAGCCTCC AGCTGAGCCC CTCATTACAG TGGAACTCTC GTACATCATC 
AATGGCACCA CCGATCCCCA CTGTGCCAGC TGGGACTACT CCAGAGCAGA TACCAACTCG 
GGGGACTGGA ACACTGAGAG CTGCCAGACC TTGGAGACCC AGGCGGCTCA CACCCGCTGC 
CAGTGCCAGC ACCTGTCCAC CTTTGCCGTC CTGGCCCAGC CACCCAAGGA CCTGACCCTG 
GAGCTGGCAG GTGCTCCCTC TGTCCCCCTG GTGATCGGCT GTGCAGTGTC CTGCATGGCT 
CTGCTCACCC TGCTGGCCAT CTATGCAGCC TTCTGGAGGT TTATAAAATC AGAACGCTCC 
ATCATCTTGC TGAACTTCTG CCTGTCCATC CTGGCTTCCA ACATTCTGAT CCTGGTGGGC 
CAGTCCCGGG TGCTGAGCAA GGGCGTATGC ACCATGACGG CTGCCTTCCT ACACTTCTTC 
TTTCTGTCCT CCTTTTGCTG GGTGCTTACA GAGGCCTGGC AATCCTATCT GGCTGTCATC 
GGGCGGATGC GCACCCGCCT GGTTCGCAAG CGCTTCCTCT GCCTGGGCTG GGGTCTACCT 
GCCCTGGTGG TGGCTGTGTC TGTTGGCTTT ACTCGCACCA AAGGATATGG TACATCCAGC 
TACTGCTGGC TGTCCCTAGA GGGCGGCCTG CTCTATGCCT TCGTGGGTCC AGCAGCAGTC 
ATTGTCCTGG TGAACATGCT CATCGGGATT ATCGTCTTCA ACAAGCTCAT GGCTCGCGAT 
GGCGTCTCAG ACAAATCTAA GAAGCAGAGG GCTGGGTCGG AGCGGTGCCC CTGGGCCAGC 
CTGCTCCTTC CCTGCTCAGC GTGTGGAGCG GTCCCCAGCC CCCTGCTCAG CTCAGCCTCG 
GCCAGGAATG CCATGGCTTC ACTCTGGAGT TCCTGCGTGG TACTGCCTCT CCTGGCGCTT 
ACCTGGATGT CTGCCGTCCT GGCCATGACA GATCGCCGCT CCGTCCTCTT CCAGGCACTC 
TTTGCCGTTT TCAACTCTGC ACAAGGCTTT GTCATCACCG CTGTGCACTG CTTCCTGCGC 
CGAGAGGTCC AGGATGTGGT AAAGTGTCAG ATGGGTGTGT GTCGGGCTGA TGAGAGTGAA 
GACTCCCCAG ACTCGTGCAA GAACGGGCAG CTGCAGATCC TGTCAGACTT TGAAAAGGAC 
GTGGATCTGG CTTGTCAGAC AGTTCTGTTC AAGGAGGTCA ACACCTGCAA CCCGTCCACC 
ATTACCGGCA CTCTGTCCCG CCTGTCTCTG GATGAGGATG AGGAGCCCAA GTCCTGTCTC 
GTGGGTCCTG AGGGTGGCCT CAGCTTCTCA CCACTGCCTG GGAACATCCT GGTACCCATG 
GCAGCCTCAC CAGGTCTAGG GGAGCCACCA CCACCCCAGG AGACCAACCC TGTGTACATG 
TGTGGGGAGG GTGGCCTGCG GCAGTTGGAC CTTACATGGA TACGGCAGAG TGAACCAGGC 
TCTGAGGGGG ACTACATGGT TCTGCCCCGG CGGACTTTGA GCCTGCAGCC TGGTGGTGGG 
GGTACAGCGG GTGAGGAGGC CCCAAGGGCC CGGCCTGAGG GGACCCCCCG GCGGGCTGCC 
AAAACGGTAG CCCACACTGA AGGCTACCCC AGCTTCCTGT CTGTGGAGCA CTCGGGTCTA 
GGGCTGGGCC CTGCCTATGG GTCTCTCCAG AACCCGTATG GAATGACCTT CCAACCACCA 
CCACCAACAC CCAGCGCCCG CCAAGTACCA GAGCCAGGAG AACGTAGCCG GACCATGCCC 
CGTACAGTGC CTGGTTCCAC CATGAAGCTG GGCTCCCTTG AGCGAAAGAA GCTTCGGTAT 
TCGGACTTGG ACTTTGAGAA GGTGATGCAC ACTCGGAAAC GGCACTCGGA ACTCTACCAC 
GAACTCAACC AGAAGTTCCA CACTTTCGAC CGCTACCGTA GCCAGTCCTC AGCCAAGGAG 
AAACCCAGCC CCCCCGGGGG ACGCCCTGGC TTGTCCCAGC ACAGGAGGCA TCAAAGCTGG 
AGCACCTTCA AATCTATGAC ACTGGGCTCA CTGCCCCCCA AGCCCCGAGA ACGGCTGGCC 
CTGCACCGGA CAGCAGCCTG GGAGCCCACA GAACCGCCAG ACGGCGACTT CCAGACAGAG 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adgrb2 ( NM_173071.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_775094.2 Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant 1, mRNA.

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CloneID	OMu14311
Clone ID Related Accession (Same CDS sequence)	NM_173071.3 , NM_173071.4
Accession Version	NM_173071.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4650bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-11
Organism	Mus musculus(house mouse)
Product	adhesion G protein-coupled receptor B2 isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL626774.23. On Dec 12, 2019 this sequence version replaced NM_173071.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC056926.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCCCAG CCTGTCCCCT CTTACTGTCT GTGATTCTGT CCCTGCGCCT GGCCACGGCC 
TTCGACCCTG CCCCCAGTGC CTGCTCTGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTC 
TCGCTGCAGG ACCTCTTTCC CACCATCGCC TCGGGCTGCT CCTGGACCCT GGAGAACCCA 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGGC AGGAGCAGGT TTGCACACAC 
TTTGCCCCGC GCCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTTACCTG CCTGCGGCCT 
GGTCCAGAGG AAGCCACAGC CCGGGCTGAG TCGGAGGTGG GACGGCCAGA GGAGGAGGAG 
GAGGAGGCGG CGGCAGCAGC ATCAGGGTTG GAGTTGTGTG GTGGCTCAGG CCCCTTTACC 
TTTCTGCACT TCGACAAGAA CTTTGTGCAG CTGTGCCTGT CGGCTGAGCC CTCTGAGGCC 
CCTCGTCTGC TAGCGCCTGC TGCCCTGGCC TTCCGTTTTG TGGAGGTCTT GCTGATCAAC 
AACAACAACT CCAGCCAGTT CACCTGTGGT GTGCTCTGCC GCTGGAGTGA GGAGTGTGGC 
CGGGCTGCAG GCAGGGCTTG TGGCTTTGCA CAGCCAGGGT GTAGTTGTCC TGGGGAGGCA 
GGGGCCAACC CCGCCACCAC CACATCTCCG GGGCCTCCGG TTGCCCACAC CCTGTCCAAT 
GCCCTGGTGC CCGGGGGCCC AGCCCCTCCT GCTGAGGCCG ACTTGCACTC GGGGAGCAGC 
AATGACCTGT TCACCACCGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG 
AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTAT ACATGGCGCA GACAGGCGAC 
CCAGCAGCTG AGGAGTGGTC CCCGTGGAGC GTGTGTTCCC TGACGTGTGG GCAGGGTCTG 
CAGGTGCGGA CCCGCTCCTG CGTGTCCTCC CCCTATGGGA CCCTGTGCAG CGGGCCCCTT 
CGGGAGACCC GGCCTTGCAA CAATTCAGCC ACCTGCCCAG TGCACGGCGT GTGGGAGGAG 
TGGGGGTCCT GGAGCCTGTG CTCCCGCAGC TGCGGGCGGG GGTCTCGGAG CCGGATGCGG 
ACCTGCGTGC CCCCCCAGCA CGGCGGCAAG GCCTGCGAGG GTCCCGAGCT GCAGACTAAA 
CTCTGCAGTA TGGCCGCCTG CCCGGTGGAA GGCCAGTGGC TAGAATGGGG TCCCTGGGGC 
CCATGCTCAT CATCTTGTGC CAATGGGACC CAGCAGCGCA GCCGGAAATG CAGTGTGGCG 
GGTCCAGCCT GGGCCACGTG CGCAGGTGCC CTCACGGATA CCCGTGAGTG CAGCAATCTC 
GATTGCCCGG CCACTGACGG CAAGTGGGGG CCGTGGAACG CGTGGAGCCT GTGCTCCAAG 
ACGTGTGACA CGGGCTGGCA ACGCCGCTTC CGCATGTGCC AGGCTTCTGG CACACAGGGC 
TACCCTTGCG AGGGCACAGG AGAGGAGGTG AAACCCTGCA GTGAGAAGAG GTGTCCAGCC 
TTCCATGAGA TGTGCAGGGA TGAGTACGTG ATGTTGATGA CATGGAAGAG GGCGGCAGCT 
GGCGAGATCA TTTACAACAA GTGTCCCCCT AATGCCTCGG GTTCTGCTAG CCGCCGCTGT 
CTCCTCAGTG CCCAGGGCGT AGCATACTGG GGACTGCCCA GCTTTGCTCG TTGCATATCC 
CATGAATACC GCTACCTGTA CCTGTCACTT CGGGAACACC TGGCTAAGGG CCAGCGCATG 
CTGGCAGGTG AGGGCATGTC ACAGGTGGTG CGGAGCCTGC AGGAGCTACT GGCACGGCGC 
ACTTACTACA GCGGGGACCT GCTCTTCTCT GTGGACATCC TAAGGAACGT CACTGACACC 
TTCAAGAGGG CCACCTATGT CCCTTCCGCC GATGACGTGC AGCGTTTCTT CCAGGTGGTG 
AGCTTCATGG TGGATTCAGA AAACAAGGAC AAATGGGATG ATGCTCAGCA GGTGTCACCG 
GGCTCTGTGC ACCTGCTGCG TGTTGTGGAA GATTTCATTC ACCTCGTGGG CGACGCTCTC 
AAGGCCTTCC AGAGCTCTCT CATTGTCACG GACAATCTGG TGATCAGCAT TCAGAGAGAG 
CCTATCTCCG CCGTGTCCAG TGACATCACG TTTCCCATGC GGGGCCGCAG GGGCATGAAG 
GACTGGGTGC GACACTCAGA GGATCGTCTC TTTCTACCCA AGGAGGTGCT CAGCCTGTCC 
TCCCCAGGAA AGCCAGCCAC ACCTGGGGCA GCCACAGCAG GCAGCCCGGG CAGGGGGAGG 
GGCCCAGGAA CGGTGCCCCC TGGCCCAGGC CACGCCCACC AGCGCCTTCT CCCAGCTGAC 
CCCGAAGAGT CCTCCTCCTA CTTTGTGATC GGTGCTGTGC TCTACCGCAC CCTTGGCCTC 
ATCCTGCCGC CCCCCAGGCC TCCACTTGCT GTCACCTCCC GGGTGATGAC AGTGACTGTG 
CGTCCCCCCA CCCAGCCTCC AGCTGAGCCC CTCATTACAG TGGAACTCTC GTACATCATC 
AATGGCACCA CCGATCCCCA CTGTGCCAGC TGGGACTACT CCAGAGCAGA TACCAACTCG 
GGGGACTGGA ACACTGAGAG CTGCCAGACC TTGGAGACCC AGGCGGCTCA CACCCGCTGC 
CAGTGCCAGC ACCTGTCCAC CTTTGCCGTC CTGGCCCAGC CACCCAAGGA CCTGACCCTG 
GAGCTGGCAG GTGCTCCCTC TGTCCCCCTG GTGATCGGCT GTGCAGTGTC CTGCATGGCT 
CTGCTCACCC TGCTGGCCAT CTATGCAGCC TTCTGGAGGT TTATAAAATC AGAACGCTCC 
ATCATCTTGC TGAACTTCTG CCTGTCCATC CTGGCTTCCA ACATTCTGAT CCTGGTGGGC 
CAGTCCCGGG TGCTGAGCAA GGGCGTATGC ACCATGACGG CTGCCTTCCT ACACTTCTTC 
TTTCTGTCCT CCTTTTGCTG GGTGCTTACA GAGGCCTGGC AATCCTATCT GGCTGTCATC 
GGGCGGATGC GCACCCGCCT GGTTCGCAAG CGCTTCCTCT GCCTGGGCTG GGGTCTACCT 
GCCCTGGTGG TGGCTGTGTC TGTTGGCTTT ACTCGCACCA AAGGATATGG TACATCCAGC 
TACTGCTGGC TGTCCCTAGA GGGCGGCCTG CTCTATGCCT TCGTGGGTCC AGCAGCAGTC 
ATTGTCCTGG TGAACATGCT CATCGGGATT ATCGTCTTCA ACAAGCTCAT GGCTCGCGAT 
GGCGTCTCAG ACAAATCTAA GAAGCAGAGG GCTGGCCGAC TGTCTTGGAA TCTCTGGGGA 
TATGGCTCTC AACTGTGTCT TTTTCCAAAG CTTCCAAGGG CTTCACTCTG GAGTTCCTGC 
GTGGTACTGC CTCTCCTGGC GCTTACCTGG ATGTCTGCCG TCCTGGCCAT GACAGATCGC 
CGCTCCGTCC TCTTCCAGGC ACTCTTTGCC GTTTTCAACT CTGCACAAGG CTTTGTCATC 
ACCGCTGTGC ACTGCTTCCT GCGCCGAGAG GTCCAGGATG TGGTAAAGTG TCAGATGGGT 
GTGTGTCGGG CTGATGAGAG TGAAGACTCC CCAGACTCGT GCAAGAACGG GCAGCTGCAG 
ATCCTGTCAG ACTTTGAAAA GGACGTGGAT CTGGCTTGTC AGACAGTTCT GTTCAAGGAG 
GTCAACACCT GCAACCCGTC CACCATTACC GGCACTCTGT CCCGCCTGTC TCTGGATGAG 
GATGAGGAGC CCAAGTCCTG TCTCGTGGGT CCTGAGGGTG GCCTCAGCTT CTCACCACTG 
CCTGGGAACA TCCTGGTACC CATGGCAGCC TCACCAGGTC TAGGGGAGCC ACCACCACCC 
CAGGAGACCA ACCCTGTGTA CATGTGTGGG GAGGGTGGCC TGCGGCAGTT GGACCTTACA 
TGGATACGGC AGAGTGAACC AGGCTCTGAG GGGGACTACA TGGTTCTGCC CCGGCGGACT 
TTGAGCCTGC AGCCTGGTGG TGGGGGTACA GCGGGTGAGG AGGCCCCAAG GGCCCGGCCT 
GAGGGGACCC CCCGGCGGGC TGCCAAAACG GTAGCCCACA CTGAAGGCTA CCCCAGCTTC 
CTGTCTGTGG AGCACTCGGG TCTAGGGCTG GGCCCTGCCT ATGGGTCTCT CCAGAACCCG 
TATGGAATGA CCTTCCAACC ACCACCACCA ACACCCAGCG CCCGCCAAGT ACCAGAGCCA 
GGAGAACGTA GCCGGACCAT GCCCCGTACA GTGCCTGGTT CCACCATGAA GCTGGGCTCC 
CTTGAGCGAA AGAAGCTTCG GTATTCGGAC TTGGACTTTG AGAAGGTGAT GCACACTCGG 
AAACGGCACT CGGAACTCTA CCACGAACTC AACCAGAAGT TCCACACTTT CGACCGCTAC 
CGTAGCCAGT CCTCAGCCAA GGAGAAACCC AGCCCCCCCG GGGGACGCCC TGGCTTGTCC 
CAGCACAGGA GGCATCAAAG CTGGAGCACC TTCAAATCTA TGACACTGGG CTCACTGCCC 
CCCAAGCCCC GAGAACGGCT GGCCCTGCAC CGGACAGCAG CCTGGGAGCC CACAGAACCG 
CCAGACGGCG ACTTCCAGAC AGAGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775094.2
CDS	454..5103
Translation	MTPACPLLLSVILSLRLATAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCTHFAPRLLPLDHYLVNFTCLRPGPEEATARAESEVGRPEEEEEEAAAAASGLELCGGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGANPATTTSPGPPVAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSSSCANGTQQRSRKCSVAGPAWATCAGALTDTRECSNLDCPATDGKWGPWNAWSLCSKTCDTGWQRRFRMCQASGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKRAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDSENKDKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPISAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSSPGKPATPGAATAGSPGRGRGPGTVPPGPGHAHQRLLPADPEESSSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADTNSGDWNTESCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGAPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNILILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGVSDKSKKQRAGRLSWNLWGYGSQLCLFPKLPRASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGGLSFSPLPGNILVPMAASPGLGEPPPPQETNPVYMCGEGGLRQLDLTWIRQSEPGSEGDYMVLPRRTLSLQPGGGGTAGEEAPRARPEGTPRRAAKTVAHTEGYPSFLSVEHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVPEPGERSRTMPRTVPGSTMKLGSLERKKLRYSDLDFEKVMHTRKRHSELYHELNQKFHTFDRYRSQSSAKEKPSPPGGRPGLSQHRRHQSWSTFKSMTLGSLPPKPRERLALHRTAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	NM_173071.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173071.4

ORF Insert Sequence:

ATGACCCCAG CCTGTCCCCT CTTACTGTCT GTGATTCTGT CCCTGCGCCT GGCCACGGCC 
TTCGACCCTG CCCCCAGTGC CTGCTCTGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTC 
TCGCTGCAGG ACCTCTTTCC CACCATCGCC TCGGGCTGCT CCTGGACCCT GGAGAACCCA 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGGC AGGAGCAGGT TTGCACACAC 
TTTGCCCCGC GCCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTTACCTG CCTGCGGCCT 
GGTCCAGAGG AAGCCACAGC CCGGGCTGAG TCGGAGGTGG GACGGCCAGA GGAGGAGGAG 
GAGGAGGCGG CGGCAGCAGC ATCAGGGTTG GAGTTGTGTG GTGGCTCAGG CCCCTTTACC 
TTTCTGCACT TCGACAAGAA CTTTGTGCAG CTGTGCCTGT CGGCTGAGCC CTCTGAGGCC 
CCTCGTCTGC TAGCGCCTGC TGCCCTGGCC TTCCGTTTTG TGGAGGTCTT GCTGATCAAC 
AACAACAACT CCAGCCAGTT CACCTGTGGT GTGCTCTGCC GCTGGAGTGA GGAGTGTGGC 
CGGGCTGCAG GCAGGGCTTG TGGCTTTGCA CAGCCAGGGT GTAGTTGTCC TGGGGAGGCA 
GGGGCCAACC CCGCCACCAC CACATCTCCG GGGCCTCCGG TTGCCCACAC CCTGTCCAAT 
GCCCTGGTGC CCGGGGGCCC AGCCCCTCCT GCTGAGGCCG ACTTGCACTC GGGGAGCAGC 
AATGACCTGT TCACCACCGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG 
AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTAT ACATGGCGCA GACAGGCGAC 
CCAGCAGCTG AGGAGTGGTC CCCGTGGAGC GTGTGTTCCC TGACGTGTGG GCAGGGTCTG 
CAGGTGCGGA CCCGCTCCTG CGTGTCCTCC CCCTATGGGA CCCTGTGCAG CGGGCCCCTT 
CGGGAGACCC GGCCTTGCAA CAATTCAGCC ACCTGCCCAG TGCACGGCGT GTGGGAGGAG 
TGGGGGTCCT GGAGCCTGTG CTCCCGCAGC TGCGGGCGGG GGTCTCGGAG CCGGATGCGG 
ACCTGCGTGC CCCCCCAGCA CGGCGGCAAG GCCTGCGAGG GTCCCGAGCT GCAGACTAAA 
CTCTGCAGTA TGGCCGCCTG CCCGGTGGAA GGCCAGTGGC TAGAATGGGG TCCCTGGGGC 
CCATGCTCAT CATCTTGTGC CAATGGGACC CAGCAGCGCA GCCGGAAATG CAGTGTGGCG 
GGTCCAGCCT GGGCCACGTG CGCAGGTGCC CTCACGGATA CCCGTGAGTG CAGCAATCTC 
GATTGCCCGG CCACTGACGG CAAGTGGGGG CCGTGGAACG CGTGGAGCCT GTGCTCCAAG 
ACGTGTGACA CGGGCTGGCA ACGCCGCTTC CGCATGTGCC AGGCTTCTGG CACACAGGGC 
TACCCTTGCG AGGGCACAGG AGAGGAGGTG AAACCCTGCA GTGAGAAGAG GTGTCCAGCC 
TTCCATGAGA TGTGCAGGGA TGAGTACGTG ATGTTGATGA CATGGAAGAG GGCGGCAGCT 
GGCGAGATCA TTTACAACAA GTGTCCCCCT AATGCCTCGG GTTCTGCTAG CCGCCGCTGT 
CTCCTCAGTG CCCAGGGCGT AGCATACTGG GGACTGCCCA GCTTTGCTCG TTGCATATCC 
CATGAATACC GCTACCTGTA CCTGTCACTT CGGGAACACC TGGCTAAGGG CCAGCGCATG 
CTGGCAGGTG AGGGCATGTC ACAGGTGGTG CGGAGCCTGC AGGAGCTACT GGCACGGCGC 
ACTTACTACA GCGGGGACCT GCTCTTCTCT GTGGACATCC TAAGGAACGT CACTGACACC 
TTCAAGAGGG CCACCTATGT CCCTTCCGCC GATGACGTGC AGCGTTTCTT CCAGGTGGTG 
AGCTTCATGG TGGATTCAGA AAACAAGGAC AAATGGGATG ATGCTCAGCA GGTGTCACCG 
GGCTCTGTGC ACCTGCTGCG TGTTGTGGAA GATTTCATTC ACCTCGTGGG CGACGCTCTC 
AAGGCCTTCC AGAGCTCTCT CATTGTCACG GACAATCTGG TGATCAGCAT TCAGAGAGAG 
CCTATCTCCG CCGTGTCCAG TGACATCACG TTTCCCATGC GGGGCCGCAG GGGCATGAAG 
GACTGGGTGC GACACTCAGA GGATCGTCTC TTTCTACCCA AGGAGGTGCT CAGCCTGTCC 
TCCCCAGGAA AGCCAGCCAC ACCTGGGGCA GCCACAGCAG GCAGCCCGGG CAGGGGGAGG 
GGCCCAGGAA CGGTGCCCCC TGGCCCAGGC CACGCCCACC AGCGCCTTCT CCCAGCTGAC 
CCCGAAGAGT CCTCCTCCTA CTTTGTGATC GGTGCTGTGC TCTACCGCAC CCTTGGCCTC 
ATCCTGCCGC CCCCCAGGCC TCCACTTGCT GTCACCTCCC GGGTGATGAC AGTGACTGTG 
CGTCCCCCCA CCCAGCCTCC AGCTGAGCCC CTCATTACAG TGGAACTCTC GTACATCATC 
AATGGCACCA CCGATCCCCA CTGTGCCAGC TGGGACTACT CCAGAGCAGA TACCAACTCG 
GGGGACTGGA ACACTGAGAG CTGCCAGACC TTGGAGACCC AGGCGGCTCA CACCCGCTGC 
CAGTGCCAGC ACCTGTCCAC CTTTGCCGTC CTGGCCCAGC CACCCAAGGA CCTGACCCTG 
GAGCTGGCAG GTGCTCCCTC TGTCCCCCTG GTGATCGGCT GTGCAGTGTC CTGCATGGCT 
CTGCTCACCC TGCTGGCCAT CTATGCAGCC TTCTGGAGGT TTATAAAATC AGAACGCTCC 
ATCATCTTGC TGAACTTCTG CCTGTCCATC CTGGCTTCCA ACATTCTGAT CCTGGTGGGC 
CAGTCCCGGG TGCTGAGCAA GGGCGTATGC ACCATGACGG CTGCCTTCCT ACACTTCTTC 
TTTCTGTCCT CCTTTTGCTG GGTGCTTACA GAGGCCTGGC AATCCTATCT GGCTGTCATC 
GGGCGGATGC GCACCCGCCT GGTTCGCAAG CGCTTCCTCT GCCTGGGCTG GGGTCTACCT 
GCCCTGGTGG TGGCTGTGTC TGTTGGCTTT ACTCGCACCA AAGGATATGG TACATCCAGC 
TACTGCTGGC TGTCCCTAGA GGGCGGCCTG CTCTATGCCT TCGTGGGTCC AGCAGCAGTC 
ATTGTCCTGG TGAACATGCT CATCGGGATT ATCGTCTTCA ACAAGCTCAT GGCTCGCGAT 
GGCGTCTCAG ACAAATCTAA GAAGCAGAGG GCTGGCCGAC TGTCTTGGAA TCTCTGGGGA 
TATGGCTCTC AACTGTGTCT TTTTCCAAAG CTTCCAAGGG CTTCACTCTG GAGTTCCTGC 
GTGGTACTGC CTCTCCTGGC GCTTACCTGG ATGTCTGCCG TCCTGGCCAT GACAGATCGC 
CGCTCCGTCC TCTTCCAGGC ACTCTTTGCC GTTTTCAACT CTGCACAAGG CTTTGTCATC 
ACCGCTGTGC ACTGCTTCCT GCGCCGAGAG GTCCAGGATG TGGTAAAGTG TCAGATGGGT 
GTGTGTCGGG CTGATGAGAG TGAAGACTCC CCAGACTCGT GCAAGAACGG GCAGCTGCAG 
ATCCTGTCAG ACTTTGAAAA GGACGTGGAT CTGGCTTGTC AGACAGTTCT GTTCAAGGAG 
GTCAACACCT GCAACCCGTC CACCATTACC GGCACTCTGT CCCGCCTGTC TCTGGATGAG 
GATGAGGAGC CCAAGTCCTG TCTCGTGGGT CCTGAGGGTG GCCTCAGCTT CTCACCACTG 
CCTGGGAACA TCCTGGTACC CATGGCAGCC TCACCAGGTC TAGGGGAGCC ACCACCACCC 
CAGGAGACCA ACCCTGTGTA CATGTGTGGG GAGGGTGGCC TGCGGCAGTT GGACCTTACA 
TGGATACGGC AGAGTGAACC AGGCTCTGAG GGGGACTACA TGGTTCTGCC CCGGCGGACT 
TTGAGCCTGC AGCCTGGTGG TGGGGGTACA GCGGGTGAGG AGGCCCCAAG GGCCCGGCCT 
GAGGGGACCC CCCGGCGGGC TGCCAAAACG GTAGCCCACA CTGAAGGCTA CCCCAGCTTC 
CTGTCTGTGG AGCACTCGGG TCTAGGGCTG GGCCCTGCCT ATGGGTCTCT CCAGAACCCG 
TATGGAATGA CCTTCCAACC ACCACCACCA ACACCCAGCG CCCGCCAAGT ACCAGAGCCA 
GGAGAACGTA GCCGGACCAT GCCCCGTACA GTGCCTGGTT CCACCATGAA GCTGGGCTCC 
CTTGAGCGAA AGAAGCTTCG GTATTCGGAC TTGGACTTTG AGAAGGTGAT GCACACTCGG 
AAACGGCACT CGGAACTCTA CCACGAACTC AACCAGAAGT TCCACACTTT CGACCGCTAC 
CGTAGCCAGT CCTCAGCCAA GGAGAAACCC AGCCCCCCCG GGGGACGCCC TGGCTTGTCC 
CAGCACAGGA GGCATCAAAG CTGGAGCACC TTCAAATCTA TGACACTGGG CTCACTGCCC 
CCCAAGCCCC GAGAACGGCT GGCCCTGCAC CGGACAGCAG CCTGGGAGCC CACAGAACCG 
CCAGACGGCG ACTTCCAGAC AGAGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adgrb2 ( NM_001377056.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001363985.1 Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant 5, mRNA.

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CloneID	OMu73516
Clone ID Related Accession (Same CDS sequence)	NM_001377056.1 , XM_011240509.1
Accession Version	NM_001377056.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4587bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-11
Organism	Mus musculus(house mouse)
Product	adhesion G protein-coupled receptor B2 isoform 5 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL626774.23. On Dec 12, 2019 this sequence version replaced XM_011240509.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: SRR7974084.21296.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849379 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

ATGACCCCAG CCTGTCCCCT CTTACTGTCT GTGATTCTGT CCCTGCGCCT GGCCACGGCC 
TTCGACCCTG CCCCCAGTGC CTGCTCTGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTC 
TCGCTGCAGG ACCTCTTTCC CACCATCGCC TCGGGCTGCT CCTGGACCCT GGAGAACCCA 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGGC AGGAGCAGGT TTGCACACAC 
TTTGCCCCGC GCCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTTACCTG CCTGCGGCCT 
GGTCCAGAGG AAGCCACAGC CCGGGCTGAG TCGGAGGTGG GACGGCCAGA GGAGGAGGAG 
GAGGAGGCGG CGGCAGCAGC ATCAGGGTTG GAGTTGTGTG GTGGCTCAGG CCCCTTTACC 
TTTCTGCACT TCGACAAGAA CTTTGTGCAG CTGTGCCTGT CGGCTGAGCC CTCTGAGGCC 
CCTCGTCTGC TAGCGCCTGC TGCCCTGGCC TTCCGTTTTG TGGAGGTCTT GCTGATCAAC 
AACAACAACT CCAGCCAGTT CACCTGTGGT GTGCTCTGCC GCTGGAGTGA GGAGTGTGGC 
CGGGCTGCAG GCAGGGCTTG TGGCTTTGCA CAGCCAGGGT GTAGTTGTCC TGGGGAGGCA 
GGGGCCAACC CCGCCACCAC CACATCTCCG GGGCCTCCGG TTGCCCACAC CCTGTCCAAT 
GCCCTGGTGC CCGGGGGCCC AGCCCCTCCT GCTGAGGCCG ACTTGCACTC GGGGAGCAGC 
AATGACCTGT TCACCACCGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG 
AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTAT ACATGGCGCA GACAGGCGAC 
CCAGCAGCTG AGGAGTGGTC CCCGTGGAGC GTGTGTTCCC TGACGTGTGG GCAGGGTCTG 
CAGGTGCGGA CCCGCTCCTG CGTGTCCTCC CCCTATGGGA CCCTGTGCAG CGGGCCCCTT 
CGGGAGACCC GGCCTTGCAA CAATTCAGCC ACCTGCCCAG TGCACGGCGT GTGGGAGGAG 
TGGGGGTCCT GGAGCCTGTG CTCCCGCAGC TGCGGGCGGG GGTCTCGGAG CCGGATGCGG 
ACCTGCGTGC CCCCCCAGCA CGGCGGCAAG GCCTGCGAGG GTCCCGAGCT GCAGACTAAA 
CTCTGCAGTA TGGCCGCCTG CCCGGTGGAA GGCCAGTGGC TAGAATGGGG TCCCTGGGGC 
CCATGCTCAT CATCTTGTGC CAATGGGACC CAGCAGCGCA GCCGGAAATG CAGTGTGGCG 
GGTCCAGCCT GGGCCACGTG CGCAGGTGCC CTCACGGATA CCCGTGAGTG CAGCAATCTC 
GATTGCCCGG CCACTGACGG CAAGTGGGGG CCGTGGAACG CGTGGAGCCT GTGCTCCAAG 
ACGTGTGACA CGGGCTGGCA ACGCCGCTTC CGCATGTGCC AGGCTTCTGG CACACAGGGC 
TACCCTTGCG AGGGCACAGG AGAGGAGGTG AAACCCTGCA GTGAGAAGAG GTGTCCAGCC 
TTCCATGAGA TGTGCAGGGA TGAGTACGTG ATGTTGATGA CATGGAAGAG GGCGGCAGCT 
GGCGAGATCA TTTACAACAA GTGTCCCCCT AATGCCTCGG GTTCTGCTAG CCGCCGCTGT 
CTCCTCAGTG CCCAGGGCGT AGCATACTGG GGACTGCCCA GCTTTGCTCG TTGCATATCC 
CATGAATACC GCTACCTGTA CCTGTCACTT CGGGAACACC TGGCTAAGGG CCAGCGCATG 
CTGGCAGGTG AGGGCATGTC ACAGGTGGTG CGGAGCCTGC AGGAGCTACT GGCACGGCGC 
ACTTACTACA GCGGGGACCT GCTCTTCTCT GTGGACATCC TAAGGAACGT CACTGACACC 
TTCAAGAGGG CCACCTATGT CCCTTCCGCC GATGACGTGC AGCGTTTCTT CCAGGTGGTG 
AGCTTCATGG TGGATTCAGA AAACAAGGAC AAATGGGATG ATGCTCAGCA GGTGTCACCG 
GGCTCTGTGC ACCTGCTGCG TGTTGTGGAA GATTTCATTC ACCTCGTGGG CGACGCTCTC 
AAGGCCTTCC AGAGCTCTCT CATTGTCACG GACAATCTGG TGATCAGCAT TCAGAGAGAG 
CCTATCTCCG CCGTGTCCAG TGACATCACG TTTCCCATGC GGGGCCGCAG GGGCATGAAG 
GACTGGGTGC GACACTCAGA GGATCGTCTC TTTCTACCCA AGGAGGTGCT CAGCCTGTCC 
TCCCCAGGAA AGCCAGCCAC ACCTGGGGCA GCCACAGCAG GCAGCCCGGG CAGGGGGAGG 
GGCCCAGGAA CGGTGCCCCC TGGCCCAGGC CACGCCCACC AGCGCCTTCT CCCAGCTGAC 
CCCGAAGAGT CCTCCTCCTA CTTTGTGATC GGTGCTGTGC TCTACCGCAC CCTTGGCCTC 
ATCCTGCCGC CCCCCAGGCC TCCACTTGCT GTCACCTCCC GGGTGATGAC AGTGACTGTG 
CGTCCCCCCA CCCAGCCTCC AGCTGAGCCC CTCATTACAG TGGAACTCTC GTACATCATC 
AATGGCACCA CCGATCCCCA CTGTGCCAGC TGGGACTACT CCAGAGCAGA TACCAACTCG 
GGGGACTGGA ACACTGAGAG CTGCCAGACC TTGGAGACCC AGGCGGCTCA CACCCGCTGC 
CAGTGCCAGC ACCTGTCCAC CTTTGCCGTC CTGGCCCAGC CACCCAAGGA CCTGACCCTG 
GAGCTGGCAG GTGCTCCCTC TGTCCCCCTG GTGATCGGCT GTGCAGTGTC CTGCATGGCT 
CTGCTCACCC TGCTGGCCAT CTATGCAGCC TTCTGGAGGT TTATAAAATC AGAACGCTCC 
ATCATCTTGC TGAACTTCTG CCTGTCCATC CTGGCTTCCA ACATTCTGAT CCTGGTGGGC 
CAGTCCCGGG TGCTGAGCAA GGGCGTATGC ACCATGACGG CTGCCTTCCT ACACTTCTTC 
TTTCTGTCCT CCTTTTGCTG GGTGCTTACA GAGGCCTGGC AATCCTATCT GGCTGTCATC 
GGGCGGATGC GCACCCGCCT GGTTCGCAAG CGCTTCCTCT GCCTGGGCTG GGGTCTACCT 
GCCCTGGTGG TGGCTGTGTC TGTTGGCTTT ACTCGCACCA AAGGATATGG TACATCCAGC 
TACTGCTGGC TGTCCCTAGA GGGCGGCCTG CTCTATGCCT TCGTGGGTCC AGCAGCAGTC 
ATTGTCCTGG TGAACATGCT CATCGGGATT ATCGTCTTCA ACAAGCTCAT GGCTCGCGAT 
GGCGTCTCAG ACAAATCTAA GAAGCAGAGG GCTGGGGCTT CACTCTGGAG TTCCTGCGTG 
GTACTGCCTC TCCTGGCGCT TACCTGGATG TCTGCCGTCC TGGCCATGAC AGATCGCCGC 
TCCGTCCTCT TCCAGGCACT CTTTGCCGTT TTCAACTCTG CACAAGGCTT TGTCATCACC 
GCTGTGCACT GCTTCCTGCG CCGAGAGGTC CAGGATGTGG TAAAGTGTCA GATGGGTGTG 
TGTCGGGCTG ATGAGAGTGA AGACTCCCCA GACTCGTGCA AGAACGGGCA GCTGCAGATC 
CTGTCAGACT TTGAAAAGGA CGTGGATCTG GCTTGTCAGA CAGTTCTGTT CAAGGAGGTC 
AACACCTGCA ACCCGTCCAC CATTACCGGC ACTCTGTCCC GCCTGTCTCT GGATGAGGAT 
GAGGAGCCCA AGTCCTGTCT CGTGGGTCCT GAGGGTGGCC TCAGCTTCTC ACCACTGCCT 
GGGAACATCC TGGTACCCAT GGCAGCCTCA CCAGGTCTAG GGGAGCCACC ACCACCCCAG 
GAGACCAACC CTGTGTACAT GTGTGGGGAG GGTGGCCTGC GGCAGTTGGA CCTTACATGG 
ATACGGCAGA GTGAACCAGG CTCTGAGGGG GACTACATGG TTCTGCCCCG GCGGACTTTG 
AGCCTGCAGC CTGGTGGTGG GGGTACAGCG GGTGAGGAGG CCCCAAGGGC CCGGCCTGAG 
GGGACCCCCC GGCGGGCTGC CAAAACGGTA GCCCACACTG AAGGCTACCC CAGCTTCCTG 
TCTGTGGAGC ACTCGGGTCT AGGGCTGGGC CCTGCCTATG GGTCTCTCCA GAACCCGTAT 
GGAATGACCT TCCAACCACC ACCACCAACA CCCAGCGCCC GCCAAGTACC AGAGCCAGGA 
GAACGTAGCC GGACCATGCC CCGTACAGTG CCTGGTTCCA CCATGAAGCT GGGCTCCCTT 
GAGCGAAAGA AGCTTCGGTA TTCGGACTTG GACTTTGAGA AGGTGATGCA CACTCGGAAA 
CGGCACTCGG AACTCTACCA CGAACTCAAC CAGAAGTTCC ACACTTTCGA CCGCTACCGT 
AGCCAGTCCT CAGCCAAGGA GAAACCCAGC CCCCCCGGGG GACGCCCTGG CTTGTCCCAG 
CACAGGAGGC ATCAAAGCTG GAGCACCTTC AAATCTATGA CACTGGGCTC ACTGCCCCCC 
AAGCCCCGAG AACGGCTGGC CCTGCACCGG ACAGCAGCCT GGGAGCCCAC AGAACCGCCA 
GACGGCGACT TCCAGACAGA GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363985.1
CDS	454..5040
Translation	MTPACPLLLSVILSLRLATAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCTHFAPRLLPLDHYLVNFTCLRPGPEEATARAESEVGRPEEEEEEAAAAASGLELCGGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGANPATTTSPGPPVAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSSSCANGTQQRSRKCSVAGPAWATCAGALTDTRECSNLDCPATDGKWGPWNAWSLCSKTCDTGWQRRFRMCQASGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKRAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDSENKDKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPISAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSSPGKPATPGAATAGSPGRGRGPGTVPPGPGHAHQRLLPADPEESSSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADTNSGDWNTESCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGAPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNILILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGVSDKSKKQRAGASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGGLSFSPLPGNILVPMAASPGLGEPPPPQETNPVYMCGEGGLRQLDLTWIRQSEPGSEGDYMVLPRRTLSLQPGGGGTAGEEAPRARPEGTPRRAAKTVAHTEGYPSFLSVEHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVPEPGERSRTMPRTVPGSTMKLGSLERKKLRYSDLDFEKVMHTRKRHSELYHELNQKFHTFDRYRSQSSAKEKPSPPGGRPGLSQHRRHQSWSTFKSMTLGSLPPKPRERLALHRTAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	NM_001377056.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001377056.1

ORF Insert Sequence:

ATGACCCCAG CCTGTCCCCT CTTACTGTCT GTGATTCTGT CCCTGCGCCT GGCCACGGCC 
TTCGACCCTG CCCCCAGTGC CTGCTCTGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTC 
TCGCTGCAGG ACCTCTTTCC CACCATCGCC TCGGGCTGCT CCTGGACCCT GGAGAACCCA 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGGC AGGAGCAGGT TTGCACACAC 
TTTGCCCCGC GCCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTTACCTG CCTGCGGCCT 
GGTCCAGAGG AAGCCACAGC CCGGGCTGAG TCGGAGGTGG GACGGCCAGA GGAGGAGGAG 
GAGGAGGCGG CGGCAGCAGC ATCAGGGTTG GAGTTGTGTG GTGGCTCAGG CCCCTTTACC 
TTTCTGCACT TCGACAAGAA CTTTGTGCAG CTGTGCCTGT CGGCTGAGCC CTCTGAGGCC 
CCTCGTCTGC TAGCGCCTGC TGCCCTGGCC TTCCGTTTTG TGGAGGTCTT GCTGATCAAC 
AACAACAACT CCAGCCAGTT CACCTGTGGT GTGCTCTGCC GCTGGAGTGA GGAGTGTGGC 
CGGGCTGCAG GCAGGGCTTG TGGCTTTGCA CAGCCAGGGT GTAGTTGTCC TGGGGAGGCA 
GGGGCCAACC CCGCCACCAC CACATCTCCG GGGCCTCCGG TTGCCCACAC CCTGTCCAAT 
GCCCTGGTGC CCGGGGGCCC AGCCCCTCCT GCTGAGGCCG ACTTGCACTC GGGGAGCAGC 
AATGACCTGT TCACCACCGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG 
AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTAT ACATGGCGCA GACAGGCGAC 
CCAGCAGCTG AGGAGTGGTC CCCGTGGAGC GTGTGTTCCC TGACGTGTGG GCAGGGTCTG 
CAGGTGCGGA CCCGCTCCTG CGTGTCCTCC CCCTATGGGA CCCTGTGCAG CGGGCCCCTT 
CGGGAGACCC GGCCTTGCAA CAATTCAGCC ACCTGCCCAG TGCACGGCGT GTGGGAGGAG 
TGGGGGTCCT GGAGCCTGTG CTCCCGCAGC TGCGGGCGGG GGTCTCGGAG CCGGATGCGG 
ACCTGCGTGC CCCCCCAGCA CGGCGGCAAG GCCTGCGAGG GTCCCGAGCT GCAGACTAAA 
CTCTGCAGTA TGGCCGCCTG CCCGGTGGAA GGCCAGTGGC TAGAATGGGG TCCCTGGGGC 
CCATGCTCAT CATCTTGTGC CAATGGGACC CAGCAGCGCA GCCGGAAATG CAGTGTGGCG 
GGTCCAGCCT GGGCCACGTG CGCAGGTGCC CTCACGGATA CCCGTGAGTG CAGCAATCTC 
GATTGCCCGG CCACTGACGG CAAGTGGGGG CCGTGGAACG CGTGGAGCCT GTGCTCCAAG 
ACGTGTGACA CGGGCTGGCA ACGCCGCTTC CGCATGTGCC AGGCTTCTGG CACACAGGGC 
TACCCTTGCG AGGGCACAGG AGAGGAGGTG AAACCCTGCA GTGAGAAGAG GTGTCCAGCC 
TTCCATGAGA TGTGCAGGGA TGAGTACGTG ATGTTGATGA CATGGAAGAG GGCGGCAGCT 
GGCGAGATCA TTTACAACAA GTGTCCCCCT AATGCCTCGG GTTCTGCTAG CCGCCGCTGT 
CTCCTCAGTG CCCAGGGCGT AGCATACTGG GGACTGCCCA GCTTTGCTCG TTGCATATCC 
CATGAATACC GCTACCTGTA CCTGTCACTT CGGGAACACC TGGCTAAGGG CCAGCGCATG 
CTGGCAGGTG AGGGCATGTC ACAGGTGGTG CGGAGCCTGC AGGAGCTACT GGCACGGCGC 
ACTTACTACA GCGGGGACCT GCTCTTCTCT GTGGACATCC TAAGGAACGT CACTGACACC 
TTCAAGAGGG CCACCTATGT CCCTTCCGCC GATGACGTGC AGCGTTTCTT CCAGGTGGTG 
AGCTTCATGG TGGATTCAGA AAACAAGGAC AAATGGGATG ATGCTCAGCA GGTGTCACCG 
GGCTCTGTGC ACCTGCTGCG TGTTGTGGAA GATTTCATTC ACCTCGTGGG CGACGCTCTC 
AAGGCCTTCC AGAGCTCTCT CATTGTCACG GACAATCTGG TGATCAGCAT TCAGAGAGAG 
CCTATCTCCG CCGTGTCCAG TGACATCACG TTTCCCATGC GGGGCCGCAG GGGCATGAAG 
GACTGGGTGC GACACTCAGA GGATCGTCTC TTTCTACCCA AGGAGGTGCT CAGCCTGTCC 
TCCCCAGGAA AGCCAGCCAC ACCTGGGGCA GCCACAGCAG GCAGCCCGGG CAGGGGGAGG 
GGCCCAGGAA CGGTGCCCCC TGGCCCAGGC CACGCCCACC AGCGCCTTCT CCCAGCTGAC 
CCCGAAGAGT CCTCCTCCTA CTTTGTGATC GGTGCTGTGC TCTACCGCAC CCTTGGCCTC 
ATCCTGCCGC CCCCCAGGCC TCCACTTGCT GTCACCTCCC GGGTGATGAC AGTGACTGTG 
CGTCCCCCCA CCCAGCCTCC AGCTGAGCCC CTCATTACAG TGGAACTCTC GTACATCATC 
AATGGCACCA CCGATCCCCA CTGTGCCAGC TGGGACTACT CCAGAGCAGA TACCAACTCG 
GGGGACTGGA ACACTGAGAG CTGCCAGACC TTGGAGACCC AGGCGGCTCA CACCCGCTGC 
CAGTGCCAGC ACCTGTCCAC CTTTGCCGTC CTGGCCCAGC CACCCAAGGA CCTGACCCTG 
GAGCTGGCAG GTGCTCCCTC TGTCCCCCTG GTGATCGGCT GTGCAGTGTC CTGCATGGCT 
CTGCTCACCC TGCTGGCCAT CTATGCAGCC TTCTGGAGGT TTATAAAATC AGAACGCTCC 
ATCATCTTGC TGAACTTCTG CCTGTCCATC CTGGCTTCCA ACATTCTGAT CCTGGTGGGC 
CAGTCCCGGG TGCTGAGCAA GGGCGTATGC ACCATGACGG CTGCCTTCCT ACACTTCTTC 
TTTCTGTCCT CCTTTTGCTG GGTGCTTACA GAGGCCTGGC AATCCTATCT GGCTGTCATC 
GGGCGGATGC GCACCCGCCT GGTTCGCAAG CGCTTCCTCT GCCTGGGCTG GGGTCTACCT 
GCCCTGGTGG TGGCTGTGTC TGTTGGCTTT ACTCGCACCA AAGGATATGG TACATCCAGC 
TACTGCTGGC TGTCCCTAGA GGGCGGCCTG CTCTATGCCT TCGTGGGTCC AGCAGCAGTC 
ATTGTCCTGG TGAACATGCT CATCGGGATT ATCGTCTTCA ACAAGCTCAT GGCTCGCGAT 
GGCGTCTCAG ACAAATCTAA GAAGCAGAGG GCTGGGGCTT CACTCTGGAG TTCCTGCGTG 
GTACTGCCTC TCCTGGCGCT TACCTGGATG TCTGCCGTCC TGGCCATGAC AGATCGCCGC 
TCCGTCCTCT TCCAGGCACT CTTTGCCGTT TTCAACTCTG CACAAGGCTT TGTCATCACC 
GCTGTGCACT GCTTCCTGCG CCGAGAGGTC CAGGATGTGG TAAAGTGTCA GATGGGTGTG 
TGTCGGGCTG ATGAGAGTGA AGACTCCCCA GACTCGTGCA AGAACGGGCA GCTGCAGATC 
CTGTCAGACT TTGAAAAGGA CGTGGATCTG GCTTGTCAGA CAGTTCTGTT CAAGGAGGTC 
AACACCTGCA ACCCGTCCAC CATTACCGGC ACTCTGTCCC GCCTGTCTCT GGATGAGGAT 
GAGGAGCCCA AGTCCTGTCT CGTGGGTCCT GAGGGTGGCC TCAGCTTCTC ACCACTGCCT 
GGGAACATCC TGGTACCCAT GGCAGCCTCA CCAGGTCTAG GGGAGCCACC ACCACCCCAG 
GAGACCAACC CTGTGTACAT GTGTGGGGAG GGTGGCCTGC GGCAGTTGGA CCTTACATGG 
ATACGGCAGA GTGAACCAGG CTCTGAGGGG GACTACATGG TTCTGCCCCG GCGGACTTTG 
AGCCTGCAGC CTGGTGGTGG GGGTACAGCG GGTGAGGAGG CCCCAAGGGC CCGGCCTGAG 
GGGACCCCCC GGCGGGCTGC CAAAACGGTA GCCCACACTG AAGGCTACCC CAGCTTCCTG 
TCTGTGGAGC ACTCGGGTCT AGGGCTGGGC CCTGCCTATG GGTCTCTCCA GAACCCGTAT 
GGAATGACCT TCCAACCACC ACCACCAACA CCCAGCGCCC GCCAAGTACC AGAGCCAGGA 
GAACGTAGCC GGACCATGCC CCGTACAGTG CCTGGTTCCA CCATGAAGCT GGGCTCCCTT 
GAGCGAAAGA AGCTTCGGTA TTCGGACTTG GACTTTGAGA AGGTGATGCA CACTCGGAAA 
CGGCACTCGG AACTCTACCA CGAACTCAAC CAGAAGTTCC ACACTTTCGA CCGCTACCGT 
AGCCAGTCCT CAGCCAAGGA GAAACCCAGC CCCCCCGGGG GACGCCCTGG CTTGTCCCAG 
CACAGGAGGC ATCAAAGCTG GAGCACCTTC AAATCTATGA CACTGGGCTC ACTGCCCCCC 
AAGCCCCGAG AACGGCTGGC CCTGCACCGG ACAGCAGCCT GGGAGCCCAC AGAACCGCCA 
GACGGCGACT TCCAGACAGA GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adgrb2 ( XM_011240507.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011238809.1 Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant X2, mRNA.

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CloneID	OMu73514
Clone ID Related Accession (Same CDS sequence)	XM_011240507.1 , XM_011240507.2
Accession Version	XM_011240507.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	adhesion G protein-coupled receptor B2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011240507.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCAG CCTGTCCCCT CTTACTGTCT GTGATTCTGT CCCTGCGCCT GGCCACGGCC 
TTCGACCCTG CCCCCAGTGC CTGCTCTGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTC 
TCGCTGCAGG ACCTCTTTCC CACCATCGCC TCGGGCTGCT CCTGGACCCT GGAGAACCCA 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGGC AGGAGCAGGT TTGCACACAC 
TTTGCCCCGC GCCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTTACCTG CCTGCGGCCT 
GGTCCAGAGG AAGCCACAGC CCGGGCTGAG TCGGAGGTGG GACGGCCAGA GGAGGAGGAG 
GAGGAGGCGG CGGCAGCAGC ATCAGGGTTG GAGTTGTGTG GTGGCTCAGG CCCCTTTACC 
TTTCTGCACT TCGACAAGAA CTTTGTGCAG CTGTGCCTGT CGGCTGAGCC CTCTGAGGCC 
CCTCGTCTGC TAGCGCCTGC TGCCCTGGCC TTCCGTTTTG TGGAGGTCTT GCTGATCAAC 
AACAACAACT CCAGCCAGTT CACCTGTGGT GTGCTCTGCC GCTGGAGTGA GGAGTGTGGC 
CGGGCTGCAG GCAGGGCTTG TGGCTTTGCA CAGCCAGGGT GTAGTTGTCC TGGGGAGGCA 
GGGGCCAACC CCGCCACCAC CACATCTCCG GGGCCTCCGG TTGCCCACAC CCTGTCCAAT 
GCCCTGGTGC CCGGGGGCCC AGCCCCTCCT GCTGAGGCCG ACTTGCACTC GGGGAGCAGC 
AATGACCTGT TCACCACCGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG 
AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTAT ACATGGCGCA GACAGGCGAC 
CCAGCAGCTG AGGAGTGGTC CCCGTGGAGC GTGTGTTCCC TGACGTGTGG GCAGGGTCTG 
CAGGTGCGGA CCCGCTCCTG CGTGTCCTCC CCCTATGGGA CCCTGTGCAG CGGGCCCCTT 
CGGGAGACCC GGCCTTGCAA CAATTCAGCC ACCTGCCCAG TGCACGGCGT GTGGGAGGAG 
TGGGGGTCCT GGAGCCTGTG CTCCCGCAGC TGCGGGCGGG GGTCTCGGAG CCGGATGCGG 
ACCTGCGTGC CCCCCCAGCA CGGCGGCAAG GCCTGCGAGG GTCCCGAGCT GCAGACTAAA 
CTCTGCAGTA TGGCCGCCTG CCCGGTGGAA GGCCAGTGGC TAGAATGGGG TCCCTGGGGC 
CCATGCTCAT CATCTTGTGC CAATGGGACC CAGCAGCGCA GCCGGAAATG CAGTGTGGCG 
GGTCCAGCCT GGGCCACGTG CGCAGGTGCC CTCACGGATA CCCGTGAGTG CAGCAATCTC 
GATTGCCCGG CCACTGACGG CAAGTGGGGG CCGTGGAACG CGTGGAGCCT GTGCTCCAAG 
ACGTGTGACA CGGGCTGGCA ACGCCGCTTC CGCATGTGCC AGGCTTCTGG CACACAGGGC 
TACCCTTGCG AGGGCACAGG AGAGGAGGTG AAACCCTGCA GTGAGAAGAG GTGTCCAGCC 
TTCCATGAGA TGTGCAGGGA TGAGTACGTG ATGTTGATGA CATGGAAGAG GGCGGCAGCT 
GGCGAGATCA TTTACAACAA GTGTCCCCCT AATGCCTCGG GTTCTGCTAG CCGCCGCTGT 
CTCCTCAGTG CCCAGGGCGT AGCATACTGG GGACTGCCCA GCTTTGCTCG TTGCATATCC 
CATGAATACC GCTACCTGTA CCTGTCACTT CGGGAACACC TGGCTAAGGG CCAGCGCATG 
CTGGCAGGTG AGGGCATGTC ACAGGTGGTG CGGAGCCTGC AGGAGCTACT GGCACGGCGC 
ACTTACTACA GCGGGGACCT GCTCTTCTCT GTGGACATCC TAAGGAACGT CACTGACACC 
TTCAAGAGGG CCACCTATGT CCCTTCCGCC GATGACGTGC AGCGTTTCTT CCAGGTGGTG 
AGCTTCATGG TGGATTCAGA AAACAAGGAC AAATGGGATG ATGCTCAGCA GGTGTCACCG 
GGCTCTGTGC ACCTGCTGCG TGTTGTGGAA GATTTCATTC ACCTCGTGGG CGACGCTCTC 
AAGGCCTTCC AGAGCTCTCT CATTGTCACG GACAATCTGG TGATCAGCAT TCAGAGAGAG 
CCTATCTCCG CCGTGTCCAG TGACATCACG TTTCCCATGC GGGGCCGCAG GGGCATGAAG 
GACTGGGTGC GACACTCAGA GGATCGTCTC TTTCTACCCA AGGAGGTGCT CAGCCTGTCC 
TCCCCAGGAA AGCCAGCCAC ACCTGGGGCA GCCACAGCAG GCAGCCCGGG CAGGGGGAGG 
GGCCCAGGAA CGGTGCCCCC TGGCCCAGGC CACGCCCACC AGCGCCTTCT CCCAGCTGAC 
CCCGAAGAGT CCTCCTCCTA CTTTGTGATC GGTGCTGTGC TCTACCGCAC CCTTGGCCTC 
ATCCTGCCGC CCCCCAGGCC TCCACTTGCT GTCACCTCCC GGGTGATGAC AGTGACTGTG 
CGTCCCCCCA CCCAGCCTCC AGCTGAGCCC CTCATTACAG TGGAACTCTC GTACATCATC 
AATGGCACCA CCGATCCCCA CTGTGCCAGC TGGGACTACT CCAGAGCAGA TACCAACTCG 
GGGGACTGGA ACACTGAGAG CTGCCAGACC TTGGAGACCC AGGCGGCTCA CACCCGCTGC 
CAGTGCCAGC ACCTGTCCAC CTTTGCCGTC CTGGCCCAGC CACCCAAGGA CCTGACCCTG 
GAGCTGGCAG GTGCTCCCTC TGTCCCCCTG GTGATCGGCT GTGCAGTGTC CTGCATGGCT 
CTGCTCACCC TGCTGGCCAT CTATGCAGCC TTCTGGAGGT TTATAAAATC AGAACGCTCC 
ATCATCTTGC TGAACTTCTG CCTGTCCATC CTGGCTTCCA ACATTCTGAT CCTGGTGGGC 
CAGTCCCGGG TGCTGAGCAA GGGCGTATGC ACCATGACGG CTGCCTTCCT ACACTTCTTC 
TTTCTGTCCT CCTTTTGCTG GGTGCTTACA GAGGCCTGGC AATCCTATCT GGCTGTCATC 
GGGCGGATGC GCACCCGCCT GGTTCGCAAG CGCTTCCTCT GCCTGGGCTG GGGTCTACCT 
GCCCTGGTGG TGGCTGTGTC TGTTGGCTTT ACTCGCACCA AAGGATATGG TACATCCAGC 
TACTGCTGGC TGTCCCTAGA GGGCGGCCTG CTCTATGCCT TCGTGGGTCC AGCAGCAGTC 
ATTGTCCTGG TGAACATGCT CATCGGGATT ATCGTCTTCA ACAAGCTCAT GGCTCGCGAT 
GGCGTCTCAG ACAAATCTAA GAAGCAGAGG GCTGGGTCGG AGCGGTGCCC CTGGGCCAGC 
CTGCTCCTTC CCTGCTCAGC GTGTGGAGCG GTCCCCAGCC CCCTGCTCAG CTCAGCCTCG 
GCCAGGAATG CCATCCGACT GTCTTGGAAT CTCTGGGGAT ATGGCTCTCA ACTGTGTCTT 
TTTCCAAAGC TTCCAAGGGC TTCACTCTGG AGTTCCTGCG TGGTACTGCC TCTCCTGGCG 
CTTACCTGGA TGTCTGCCGT CCTGGCCATG ACAGATCGCC GCTCCGTCCT CTTCCAGGCA 
CTCTTTGCCG TTTTCAACTC TGCACAAGGC TTTGTCATCA CCGCTGTGCA CTGCTTCCTG 
CGCCGAGAGG TCCAGGATGT GGTAAAGTGT CAGATGGGTG TGTGTCGGGC TGATGAGAGT 
GAAGACTCCC CAGACTCGTG CAAGAACGGG CAGCTGCAGA TCCTGTCAGA CTTTGAAAAG 
GACGTGGATC TGGCTTGTCA GACAGTTCTG TTCAAGGAGG TCAACACCTG CAACCCGTCC 
ACCATTACCG GCACTCTGTC CCGCCTGTCT CTGGATGAGG ATGAGGAGCC CAAGTCCTGT 
CTCGTGGGTC CTGAGGGTGG CCTCAGCTTC TCACCACTGC CTGGGAACAT CCTGGTACCC 
ATGGCAGCCT CACCAGGTCT AGGGGAGCCA CCACCACCCC AGGAGACCAA CCCTGTGTAC 
ATGTGTGGGG AGGGTGGCCT GCGGCAGTTG GACCTTACAT GGATACGGCA GAGTGAACCA 
GGCTCTGAGG GGGACTACAT GGTTCTGCCC CGGCGGACTT TGAGCCTGCA GCCTGGTGGT 
GGGGGTACAG CGGGTGAGGA GGCCCCAAGG GCCCGGCCTG AGGGGACCCC CCGGCGGGCT 
GCCAAAACGG TAGCCCACAC TGAAGGCTAC CCCAGCTTCC TGTCTGTGGA GCACTCGGGT 
CTAGGGCTGG GCCCTGCCTA TGGGTCTCTC CAGAACCCGT ATGGAATGAC CTTCCAACCA 
CCACCACCAA CACCCAGCGC CCGCCAAGTA CCAGAGCCAG GAGAACGTAG CCGGACCATG 
CCCCGTACAG TGCCTGGTTC CACCATGAAG CTGGGCTCCC TTGAGCGAAA GAAGCTTCGG 
TATTCGGACT TGGACTTTGA GAAGGTGATG CACACTCGGA AACGGCACTC GGAACTCTAC 
CACGAACTCA ACCAGAAGTT CCACACTTTC GACCGCTACC GTAGCCAGTC CTCAGCCAAG 
GAGAAACCCA GCCCCCCCGG GGGACGCCCT GGCTTGTCCC AGCACAGGAG GCATCAAAGC 
TGGAGCACCT TCAAATCTAT GACACTGGGC TCACTGCCCC CCAAGCCCCG AGAACGGCTG 
GCCCTGCACC GGACAGCAGC CTGGGAGCCC ACAGAACCGC CAGACGGCGA CTTCCAGACA 
GAGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011238809.1
CDS	551..5299
Translation	MTPACPLLLSVILSLRLATAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCTHFAPRLLPLDHYLVNFTCLRPGPEEATARAESEVGRPEEEEEEAAAAASGLELCGGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGANPATTTSPGPPVAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSSSCANGTQQRSRKCSVAGPAWATCAGALTDTRECSNLDCPATDGKWGPWNAWSLCSKTCDTGWQRRFRMCQASGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKRAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDSENKDKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPISAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSSPGKPATPGAATAGSPGRGRGPGTVPPGPGHAHQRLLPADPEESSSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADTNSGDWNTESCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGAPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNILILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGVSDKSKKQRAGSERCPWASLLLPCSACGAVPSPLLSSASARNAIRLSWNLWGYGSQLCLFPKLPRASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGGLSFSPLPGNILVPMAASPGLGEPPPPQETNPVYMCGEGGLRQLDLTWIRQSEPGSEGDYMVLPRRTLSLQPGGGGTAGEEAPRARPEGTPRRAAKTVAHTEGYPSFLSVEHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVPEPGERSRTMPRTVPGSTMKLGSLERKKLRYSDLDFEKVMHTRKRHSELYHELNQKFHTFDRYRSQSSAKEKPSPPGGRPGLSQHRRHQSWSTFKSMTLGSLPPKPRERLALHRTAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_011240507.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240507.2

ORF Insert Sequence:

ATGACCCCAG CCTGTCCCCT CTTACTGTCT GTGATTCTGT CCCTGCGCCT GGCCACGGCC 
TTCGACCCTG CCCCCAGTGC CTGCTCTGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTC 
TCGCTGCAGG ACCTCTTTCC CACCATCGCC TCGGGCTGCT CCTGGACCCT GGAGAACCCA 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGGC AGGAGCAGGT TTGCACACAC 
TTTGCCCCGC GCCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTTACCTG CCTGCGGCCT 
GGTCCAGAGG AAGCCACAGC CCGGGCTGAG TCGGAGGTGG GACGGCCAGA GGAGGAGGAG 
GAGGAGGCGG CGGCAGCAGC ATCAGGGTTG GAGTTGTGTG GTGGCTCAGG CCCCTTTACC 
TTTCTGCACT TCGACAAGAA CTTTGTGCAG CTGTGCCTGT CGGCTGAGCC CTCTGAGGCC 
CCTCGTCTGC TAGCGCCTGC TGCCCTGGCC TTCCGTTTTG TGGAGGTCTT GCTGATCAAC 
AACAACAACT CCAGCCAGTT CACCTGTGGT GTGCTCTGCC GCTGGAGTGA GGAGTGTGGC 
CGGGCTGCAG GCAGGGCTTG TGGCTTTGCA CAGCCAGGGT GTAGTTGTCC TGGGGAGGCA 
GGGGCCAACC CCGCCACCAC CACATCTCCG GGGCCTCCGG TTGCCCACAC CCTGTCCAAT 
GCCCTGGTGC CCGGGGGCCC AGCCCCTCCT GCTGAGGCCG ACTTGCACTC GGGGAGCAGC 
AATGACCTGT TCACCACCGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG 
AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTAT ACATGGCGCA GACAGGCGAC 
CCAGCAGCTG AGGAGTGGTC CCCGTGGAGC GTGTGTTCCC TGACGTGTGG GCAGGGTCTG 
CAGGTGCGGA CCCGCTCCTG CGTGTCCTCC CCCTATGGGA CCCTGTGCAG CGGGCCCCTT 
CGGGAGACCC GGCCTTGCAA CAATTCAGCC ACCTGCCCAG TGCACGGCGT GTGGGAGGAG 
TGGGGGTCCT GGAGCCTGTG CTCCCGCAGC TGCGGGCGGG GGTCTCGGAG CCGGATGCGG 
ACCTGCGTGC CCCCCCAGCA CGGCGGCAAG GCCTGCGAGG GTCCCGAGCT GCAGACTAAA 
CTCTGCAGTA TGGCCGCCTG CCCGGTGGAA GGCCAGTGGC TAGAATGGGG TCCCTGGGGC 
CCATGCTCAT CATCTTGTGC CAATGGGACC CAGCAGCGCA GCCGGAAATG CAGTGTGGCG 
GGTCCAGCCT GGGCCACGTG CGCAGGTGCC CTCACGGATA CCCGTGAGTG CAGCAATCTC 
GATTGCCCGG CCACTGACGG CAAGTGGGGG CCGTGGAACG CGTGGAGCCT GTGCTCCAAG 
ACGTGTGACA CGGGCTGGCA ACGCCGCTTC CGCATGTGCC AGGCTTCTGG CACACAGGGC 
TACCCTTGCG AGGGCACAGG AGAGGAGGTG AAACCCTGCA GTGAGAAGAG GTGTCCAGCC 
TTCCATGAGA TGTGCAGGGA TGAGTACGTG ATGTTGATGA CATGGAAGAG GGCGGCAGCT 
GGCGAGATCA TTTACAACAA GTGTCCCCCT AATGCCTCGG GTTCTGCTAG CCGCCGCTGT 
CTCCTCAGTG CCCAGGGCGT AGCATACTGG GGACTGCCCA GCTTTGCTCG TTGCATATCC 
CATGAATACC GCTACCTGTA CCTGTCACTT CGGGAACACC TGGCTAAGGG CCAGCGCATG 
CTGGCAGGTG AGGGCATGTC ACAGGTGGTG CGGAGCCTGC AGGAGCTACT GGCACGGCGC 
ACTTACTACA GCGGGGACCT GCTCTTCTCT GTGGACATCC TAAGGAACGT CACTGACACC 
TTCAAGAGGG CCACCTATGT CCCTTCCGCC GATGACGTGC AGCGTTTCTT CCAGGTGGTG 
AGCTTCATGG TGGATTCAGA AAACAAGGAC AAATGGGATG ATGCTCAGCA GGTGTCACCG 
GGCTCTGTGC ACCTGCTGCG TGTTGTGGAA GATTTCATTC ACCTCGTGGG CGACGCTCTC 
AAGGCCTTCC AGAGCTCTCT CATTGTCACG GACAATCTGG TGATCAGCAT TCAGAGAGAG 
CCTATCTCCG CCGTGTCCAG TGACATCACG TTTCCCATGC GGGGCCGCAG GGGCATGAAG 
GACTGGGTGC GACACTCAGA GGATCGTCTC TTTCTACCCA AGGAGGTGCT CAGCCTGTCC 
TCCCCAGGAA AGCCAGCCAC ACCTGGGGCA GCCACAGCAG GCAGCCCGGG CAGGGGGAGG 
GGCCCAGGAA CGGTGCCCCC TGGCCCAGGC CACGCCCACC AGCGCCTTCT CCCAGCTGAC 
CCCGAAGAGT CCTCCTCCTA CTTTGTGATC GGTGCTGTGC TCTACCGCAC CCTTGGCCTC 
ATCCTGCCGC CCCCCAGGCC TCCACTTGCT GTCACCTCCC GGGTGATGAC AGTGACTGTG 
CGTCCCCCCA CCCAGCCTCC AGCTGAGCCC CTCATTACAG TGGAACTCTC GTACATCATC 
AATGGCACCA CCGATCCCCA CTGTGCCAGC TGGGACTACT CCAGAGCAGA TACCAACTCG 
GGGGACTGGA ACACTGAGAG CTGCCAGACC TTGGAGACCC AGGCGGCTCA CACCCGCTGC 
CAGTGCCAGC ACCTGTCCAC CTTTGCCGTC CTGGCCCAGC CACCCAAGGA CCTGACCCTG 
GAGCTGGCAG GTGCTCCCTC TGTCCCCCTG GTGATCGGCT GTGCAGTGTC CTGCATGGCT 
CTGCTCACCC TGCTGGCCAT CTATGCAGCC TTCTGGAGGT TTATAAAATC AGAACGCTCC 
ATCATCTTGC TGAACTTCTG CCTGTCCATC CTGGCTTCCA ACATTCTGAT CCTGGTGGGC 
CAGTCCCGGG TGCTGAGCAA GGGCGTATGC ACCATGACGG CTGCCTTCCT ACACTTCTTC 
TTTCTGTCCT CCTTTTGCTG GGTGCTTACA GAGGCCTGGC AATCCTATCT GGCTGTCATC 
GGGCGGATGC GCACCCGCCT GGTTCGCAAG CGCTTCCTCT GCCTGGGCTG GGGTCTACCT 
GCCCTGGTGG TGGCTGTGTC TGTTGGCTTT ACTCGCACCA AAGGATATGG TACATCCAGC 
TACTGCTGGC TGTCCCTAGA GGGCGGCCTG CTCTATGCCT TCGTGGGTCC AGCAGCAGTC 
ATTGTCCTGG TGAACATGCT CATCGGGATT ATCGTCTTCA ACAAGCTCAT GGCTCGCGAT 
GGCGTCTCAG ACAAATCTAA GAAGCAGAGG GCTGGGTCGG AGCGGTGCCC CTGGGCCAGC 
CTGCTCCTTC CCTGCTCAGC GTGTGGAGCG GTCCCCAGCC CCCTGCTCAG CTCAGCCTCG 
GCCAGGAATG CCATCCGACT GTCTTGGAAT CTCTGGGGAT ATGGCTCTCA ACTGTGTCTT 
TTTCCAAAGC TTCCAAGGGC TTCACTCTGG AGTTCCTGCG TGGTACTGCC TCTCCTGGCG 
CTTACCTGGA TGTCTGCCGT CCTGGCCATG ACAGATCGCC GCTCCGTCCT CTTCCAGGCA 
CTCTTTGCCG TTTTCAACTC TGCACAAGGC TTTGTCATCA CCGCTGTGCA CTGCTTCCTG 
CGCCGAGAGG TCCAGGATGT GGTAAAGTGT CAGATGGGTG TGTGTCGGGC TGATGAGAGT 
GAAGACTCCC CAGACTCGTG CAAGAACGGG CAGCTGCAGA TCCTGTCAGA CTTTGAAAAG 
GACGTGGATC TGGCTTGTCA GACAGTTCTG TTCAAGGAGG TCAACACCTG CAACCCGTCC 
ACCATTACCG GCACTCTGTC CCGCCTGTCT CTGGATGAGG ATGAGGAGCC CAAGTCCTGT 
CTCGTGGGTC CTGAGGGTGG CCTCAGCTTC TCACCACTGC CTGGGAACAT CCTGGTACCC 
ATGGCAGCCT CACCAGGTCT AGGGGAGCCA CCACCACCCC AGGAGACCAA CCCTGTGTAC 
ATGTGTGGGG AGGGTGGCCT GCGGCAGTTG GACCTTACAT GGATACGGCA GAGTGAACCA 
GGCTCTGAGG GGGACTACAT GGTTCTGCCC CGGCGGACTT TGAGCCTGCA GCCTGGTGGT 
GGGGGTACAG CGGGTGAGGA GGCCCCAAGG GCCCGGCCTG AGGGGACCCC CCGGCGGGCT 
GCCAAAACGG TAGCCCACAC TGAAGGCTAC CCCAGCTTCC TGTCTGTGGA GCACTCGGGT 
CTAGGGCTGG GCCCTGCCTA TGGGTCTCTC CAGAACCCGT ATGGAATGAC CTTCCAACCA 
CCACCACCAA CACCCAGCGC CCGCCAAGTA CCAGAGCCAG GAGAACGTAG CCGGACCATG 
CCCCGTACAG TGCCTGGTTC CACCATGAAG CTGGGCTCCC TTGAGCGAAA GAAGCTTCGG 
TATTCGGACT TGGACTTTGA GAAGGTGATG CACACTCGGA AACGGCACTC GGAACTCTAC 
CACGAACTCA ACCAGAAGTT CCACACTTTC GACCGCTACC GTAGCCAGTC CTCAGCCAAG 
GAGAAACCCA GCCCCCCCGG GGGACGCCCT GGCTTGTCCC AGCACAGGAG GCATCAAAGC 
TGGAGCACCT TCAAATCTAT GACACTGGGC TCACTGCCCC CCAAGCCCCG AGAACGGCTG 
GCCCTGCACC GGACAGCAGC CTGGGAGCCC ACAGAACCGC CAGACGGCGA CTTCCAGACA 
GAGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adgrb2 ( XM_030253457.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030109317.1 Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant X7, mRNA.

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CloneID	OMu73517
Clone ID Related Accession (Same CDS sequence)	XM_011240510.1 , XM_030253457.1
Accession Version	XM_030253457.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4584bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	adhesion G protein-coupled receptor B2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCAG CCTGTCCCCT CTTACTGTCT GTGATTCTGT CCCTGCGCCT GGCCACGGCC 
TTCGACCCTG CCCCCAGTGC CTGCTCTGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTC 
TCGCTGCAGG ACCTCTTTCC CACCATCGCC TCGGGCTGCT CCTGGACCCT GGAGAACCCA 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGGC AGGAGCAGGT TTGCACACAC 
TTTGCCCCGC GCCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTTACCTG CCTGCGGCCT 
GGTCCAGAGG AAGCCACAGC CCGGGCTGAG TCGGAGGTGG GACGGCCAGA GGAGGAGGAG 
GAGGAGGCGG CGGCAGCAGC ATCAGGGTTG GAGTTGTGTG GTGGCTCAGG CCCCTTTACC 
TTTCTGCACT TCGACAAGAA CTTTGTGCAG CTGTGCCTGT CGGCTGAGCC CTCTGAGGCC 
CCTCGTCTGC TAGCGCCTGC TGCCCTGGCC TTCCGTTTTG TGGAGGTCTT GCTGATCAAC 
AACAACAACT CCAGCCAGTT CACCTGTGGT GTGCTCTGCC GCTGGAGTGA GGAGTGTGGC 
CGGGCTGCAG GCAGGGCTTG TGGCTTTGCA CAGCCAGGGT GTAGTTGTCC TGGGGAGGCA 
GGGGCCAACC CCGCCACCAC CACATCTCCG GGGCCTCCGG TTGCCCACAC CCTGTCCAAT 
GCCCTGGTGC CCGGGGGCCC AGCCCCTCCT GCTGAGGCCG ACTTGCACTC GGGGAGCAGC 
AATGACCTGT TCACCACCGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG 
AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTAT ACATGGCGCA GACAGGCGAC 
CCAGCAGCTG AGGAGTGGTC CCCGTGGAGC GTGTGTTCCC TGACGTGTGG GCAGGGTCTG 
CAGGTGCGGA CCCGCTCCTG CGTGTCCTCC CCCTATGGGA CCCTGTGCAG CGGGCCCCTT 
CGGGAGACCC GGCCTTGCAA CAATTCAGCC ACCTGCCCAG TGGAAGGCCA GTGGCTAGAA 
TGGGGTCCCT GGGGCCCATG CTCATCATCT TGTGCCAATG GGACCCAGCA GCGCAGCCGG 
AAATGCAGTG TGGCGGGTCC AGCCTGGGCC ACGTGCGCAG GTGCCCTCAC GGATACCCGT 
GAGTGCAGCA ATCTCGATTG CCCGGCCACT GACGGCAAGT GGGGGCCGTG GAACGCGTGG 
AGCCTGTGCT CCAAGACGTG TGACACGGGC TGGCAACGCC GCTTCCGCAT GTGCCAGGCT 
TCTGGCACAC AGGGCTACCC TTGCGAGGGC ACAGGAGAGG AGGTGAAACC CTGCAGTGAG 
AAGAGGTGTC CAGCCTTCCA TGAGATGTGC AGGGATGAGT ACGTGATGTT GATGACATGG 
AAGAGGGCGG CAGCTGGCGA GATCATTTAC AACAAGTGTC CCCCTAATGC CTCGGGTTCT 
GCTAGCCGCC GCTGTCTCCT CAGTGCCCAG GGCGTAGCAT ACTGGGGACT GCCCAGCTTT 
GCTCGTTGCA TATCCCATGA ATACCGCTAC CTGTACCTGT CACTTCGGGA ACACCTGGCT 
AAGGGCCAGC GCATGCTGGC AGGTGAGGGC ATGTCACAGG TGGTGCGGAG CCTGCAGGAG 
CTACTGGCAC GGCGCACTTA CTACAGCGGG GACCTGCTCT TCTCTGTGGA CATCCTAAGG 
AACGTCACTG ACACCTTCAA GAGGGCCACC TATGTCCCTT CCGCCGATGA CGTGCAGCGT 
TTCTTCCAGG TGGTGAGCTT CATGGTGGAT TCAGAAAACA AGGACAAATG GGATGATGCT 
CAGCAGGTGT CACCGGGCTC TGTGCACCTG CTGCGTGTTG TGGAAGATTT CATTCACCTC 
GTGGGCGACG CTCTCAAGGC CTTCCAGAGC TCTCTCATTG TCACGGACAA TCTGGTGATC 
AGCATTCAGA GAGAGCCTAT CTCCGCCGTG TCCAGTGACA TCACGTTTCC CATGCGGGGC 
CGCAGGGGCA TGAAGGACTG GGTGCGACAC TCAGAGGATC GTCTCTTTCT ACCCAAGGAG 
GTGCTCAGCC TGTCCTCCCC AGGAAAGCCA GCCACACCTG GGGCAGCCAC AGCAGGCAGC 
CCGGGCAGGG GGAGGGGCCC AGGAACGGTG CCCCCTGGCC CAGGCCACGC CCACCAGCGC 
CTTCTCCCAG CTGACCCCGA AGAGTCCTCC TCCTACTTTG TGATCGGTGC TGTGCTCTAC 
CGCACCCTTG GCCTCATCCT GCCGCCCCCC AGGCCTCCAC TTGCTGTCAC CTCCCGGGTG 
ATGACAGTGA CTGTGCGTCC CCCCACCCAG CCTCCAGCTG AGCCCCTCAT TACAGTGGAA 
CTCTCGTACA TCATCAATGG CACCACCGAT CCCCACTGTG CCAGCTGGGA CTACTCCAGA 
GCAGATACCA ACTCGGGGGA CTGGAACACT GAGAGCTGCC AGACCTTGGA GACCCAGGCG 
GCTCACACCC GCTGCCAGTG CCAGCACCTG TCCACCTTTG CCGTCCTGGC CCAGCCACCC 
AAGGACCTGA CCCTGGAGCT GGCAGGTGCT CCCTCTGTCC CCCTGGTGAT CGGCTGTGCA 
GTGTCCTGCA TGGCTCTGCT CACCCTGCTG GCCATCTATG CAGCCTTCTG GAGGTTTATA 
AAATCAGAAC GCTCCATCAT CTTGCTGAAC TTCTGCCTGT CCATCCTGGC TTCCAACATT 
CTGATCCTGG TGGGCCAGTC CCGGGTGCTG AGCAAGGGCG TATGCACCAT GACGGCTGCC 
TTCCTACACT TCTTCTTTCT GTCCTCCTTT TGCTGGGTGC TTACAGAGGC CTGGCAATCC 
TATCTGGCTG TCATCGGGCG GATGCGCACC CGCCTGGTTC GCAAGCGCTT CCTCTGCCTG 
GGCTGGGGTC TACCTGCCCT GGTGGTGGCT GTGTCTGTTG GCTTTACTCG CACCAAAGGA 
TATGGTACAT CCAGCTACTG CTGGCTGTCC CTAGAGGGCG GCCTGCTCTA TGCCTTCGTG 
GGTCCAGCAG CAGTCATTGT CCTGGTGAAC ATGCTCATCG GGATTATCGT CTTCAACAAG 
CTCATGGCTC GCGATGGCGT CTCAGACAAA TCTAAGAAGC AGAGGGCTGG GTCGGAGCGG 
TGCCCCTGGG CCAGCCTGCT CCTTCCCTGC TCAGCGTGTG GAGCGGTCCC CAGCCCCCTG 
CTCAGCTCAG CCTCGGCCAG GAATGCCATC CGACTGTCTT GGAATCTCTG GGGATATGGC 
TCTCAACTGT GTCTTTTTCC AAAGCTTCCA AGGGCTTCAC TCTGGAGTTC CTGCGTGGTA 
CTGCCTCTCC TGGCGCTTAC CTGGATGTCT GCCGTCCTGG CCATGACAGA TCGCCGCTCC 
GTCCTCTTCC AGGCACTCTT TGCCGTTTTC AACTCTGCAC AAGGCTTTGT CATCACCGCT 
GTGCACTGCT TCCTGCGCCG AGAGGTCCAG GATGTGGTAA AGTGTCAGAT GGGTGTGTGT 
CGGGCTGATG AGAGTGAAGA CTCCCCAGAC TCGTGCAAGA ACGGGCAGCT GCAGATCCTG 
TCAGACTTTG AAAAGGACGT GGATCTGGCT TGTCAGACAG TTCTGTTCAA GGAGGTCAAC 
ACCTGCAACC CGTCCACCAT TACCGGCACT CTGTCCCGCC TGTCTCTGGA TGAGGATGAG 
GAGCCCAAGT CCTGTCTCGT GGGTCCTGAG GGTGGCCTCA GCTTCTCACC ACTGCCTGGG 
AACATCCTGG TACCCATGGC AGCCTCACCA GGTCTAGGGG AGCCACCACC ACCCCAGGAG 
ACCAACCCTG TGTACATGTG TGGGGAGGGT GGCCTGCGGC AGTTGGACCT TACATGGATA 
CGGCAGAGTG AACCAGGCTC TGAGGGGGAC TACATGGTTC TGCCCCGGCG GACTTTGAGC 
CTGCAGCCTG GTGGTGGGGG TACAGCGGGT GAGGAGGCCC CAAGGGCCCG GCCTGAGGGG 
ACCCCCCGGC GGGCTGCCAA AACGGTAGCC CACACTGAAG GCTACCCCAG CTTCCTGTCT 
GTGGAGCACT CGGGTCTAGG GCTGGGCCCT GCCTATGGGT CTCTCCAGAA CCCGTATGGA 
ATGACCTTCC AACCACCACC ACCAACACCC AGCGCCCGCC AAGTACCAGA GCCAGGAGAA 
CGTAGCCGGA CCATGCCCCG TACAGTGCCT GGTTCCACCA TGAAGCTGGG CTCCCTTGAG 
CGAAAGAAGC TTCGGTATTC GGACTTGGAC TTTGAGAAGG TGATGCACAC TCGGAAACGG 
CACTCGGAAC TCTACCACGA ACTCAACCAG AAGTTCCACA CTTTCGACCG CTACCGTAGC 
CAGTCCTCAG CCAAGGAGAA ACCCAGCCCC CCCGGGGGAC GCCCTGGCTT GTCCCAGCAC 
AGGAGGCATC AAAGCTGGAG CACCTTCAAA TCTATGACAC TGGGCTCACT GCCCCCCAAG 
CCCCGAGAAC GGCTGGCCCT GCACCGGACA GCAGCCTGGG AGCCCACAGA ACCGCCAGAC 
GGCGACTTCC AGACAGAGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030109317.1
CDS	551..5134
Translation	MTPACPLLLSVILSLRLATAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCTHFAPRLLPLDHYLVNFTCLRPGPEEATARAESEVGRPEEEEEEAAAAASGLELCGGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGANPATTTSPGPPVAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVEGQWLEWGPWGPCSSSCANGTQQRSRKCSVAGPAWATCAGALTDTRECSNLDCPATDGKWGPWNAWSLCSKTCDTGWQRRFRMCQASGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKRAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDSENKDKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPISAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSSPGKPATPGAATAGSPGRGRGPGTVPPGPGHAHQRLLPADPEESSSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADTNSGDWNTESCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGAPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNILILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGVSDKSKKQRAGSERCPWASLLLPCSACGAVPSPLLSSASARNAIRLSWNLWGYGSQLCLFPKLPRASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGGLSFSPLPGNILVPMAASPGLGEPPPPQETNPVYMCGEGGLRQLDLTWIRQSEPGSEGDYMVLPRRTLSLQPGGGGTAGEEAPRARPEGTPRRAAKTVAHTEGYPSFLSVEHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVPEPGERSRTMPRTVPGSTMKLGSLERKKLRYSDLDFEKVMHTRKRHSELYHELNQKFHTFDRYRSQSSAKEKPSPPGGRPGLSQHRRHQSWSTFKSMTLGSLPPKPRERLALHRTAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_030253457.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030253457.1

ORF Insert Sequence:

ATGACCCCAG CCTGTCCCCT CTTACTGTCT GTGATTCTGT CCCTGCGCCT GGCCACGGCC 
TTCGACCCTG CCCCCAGTGC CTGCTCTGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTC 
TCGCTGCAGG ACCTCTTTCC CACCATCGCC TCGGGCTGCT CCTGGACCCT GGAGAACCCA 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGGC AGGAGCAGGT TTGCACACAC 
TTTGCCCCGC GCCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTTACCTG CCTGCGGCCT 
GGTCCAGAGG AAGCCACAGC CCGGGCTGAG TCGGAGGTGG GACGGCCAGA GGAGGAGGAG 
GAGGAGGCGG CGGCAGCAGC ATCAGGGTTG GAGTTGTGTG GTGGCTCAGG CCCCTTTACC 
TTTCTGCACT TCGACAAGAA CTTTGTGCAG CTGTGCCTGT CGGCTGAGCC CTCTGAGGCC 
CCTCGTCTGC TAGCGCCTGC TGCCCTGGCC TTCCGTTTTG TGGAGGTCTT GCTGATCAAC 
AACAACAACT CCAGCCAGTT CACCTGTGGT GTGCTCTGCC GCTGGAGTGA GGAGTGTGGC 
CGGGCTGCAG GCAGGGCTTG TGGCTTTGCA CAGCCAGGGT GTAGTTGTCC TGGGGAGGCA 
GGGGCCAACC CCGCCACCAC CACATCTCCG GGGCCTCCGG TTGCCCACAC CCTGTCCAAT 
GCCCTGGTGC CCGGGGGCCC AGCCCCTCCT GCTGAGGCCG ACTTGCACTC GGGGAGCAGC 
AATGACCTGT TCACCACCGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG 
AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTAT ACATGGCGCA GACAGGCGAC 
CCAGCAGCTG AGGAGTGGTC CCCGTGGAGC GTGTGTTCCC TGACGTGTGG GCAGGGTCTG 
CAGGTGCGGA CCCGCTCCTG CGTGTCCTCC CCCTATGGGA CCCTGTGCAG CGGGCCCCTT 
CGGGAGACCC GGCCTTGCAA CAATTCAGCC ACCTGCCCAG TGGAAGGCCA GTGGCTAGAA 
TGGGGTCCCT GGGGCCCATG CTCATCATCT TGTGCCAATG GGACCCAGCA GCGCAGCCGG 
AAATGCAGTG TGGCGGGTCC AGCCTGGGCC ACGTGCGCAG GTGCCCTCAC GGATACCCGT 
GAGTGCAGCA ATCTCGATTG CCCGGCCACT GACGGCAAGT GGGGGCCGTG GAACGCGTGG 
AGCCTGTGCT CCAAGACGTG TGACACGGGC TGGCAACGCC GCTTCCGCAT GTGCCAGGCT 
TCTGGCACAC AGGGCTACCC TTGCGAGGGC ACAGGAGAGG AGGTGAAACC CTGCAGTGAG 
AAGAGGTGTC CAGCCTTCCA TGAGATGTGC AGGGATGAGT ACGTGATGTT GATGACATGG 
AAGAGGGCGG CAGCTGGCGA GATCATTTAC AACAAGTGTC CCCCTAATGC CTCGGGTTCT 
GCTAGCCGCC GCTGTCTCCT CAGTGCCCAG GGCGTAGCAT ACTGGGGACT GCCCAGCTTT 
GCTCGTTGCA TATCCCATGA ATACCGCTAC CTGTACCTGT CACTTCGGGA ACACCTGGCT 
AAGGGCCAGC GCATGCTGGC AGGTGAGGGC ATGTCACAGG TGGTGCGGAG CCTGCAGGAG 
CTACTGGCAC GGCGCACTTA CTACAGCGGG GACCTGCTCT TCTCTGTGGA CATCCTAAGG 
AACGTCACTG ACACCTTCAA GAGGGCCACC TATGTCCCTT CCGCCGATGA CGTGCAGCGT 
TTCTTCCAGG TGGTGAGCTT CATGGTGGAT TCAGAAAACA AGGACAAATG GGATGATGCT 
CAGCAGGTGT CACCGGGCTC TGTGCACCTG CTGCGTGTTG TGGAAGATTT CATTCACCTC 
GTGGGCGACG CTCTCAAGGC CTTCCAGAGC TCTCTCATTG TCACGGACAA TCTGGTGATC 
AGCATTCAGA GAGAGCCTAT CTCCGCCGTG TCCAGTGACA TCACGTTTCC CATGCGGGGC 
CGCAGGGGCA TGAAGGACTG GGTGCGACAC TCAGAGGATC GTCTCTTTCT ACCCAAGGAG 
GTGCTCAGCC TGTCCTCCCC AGGAAAGCCA GCCACACCTG GGGCAGCCAC AGCAGGCAGC 
CCGGGCAGGG GGAGGGGCCC AGGAACGGTG CCCCCTGGCC CAGGCCACGC CCACCAGCGC 
CTTCTCCCAG CTGACCCCGA AGAGTCCTCC TCCTACTTTG TGATCGGTGC TGTGCTCTAC 
CGCACCCTTG GCCTCATCCT GCCGCCCCCC AGGCCTCCAC TTGCTGTCAC CTCCCGGGTG 
ATGACAGTGA CTGTGCGTCC CCCCACCCAG CCTCCAGCTG AGCCCCTCAT TACAGTGGAA 
CTCTCGTACA TCATCAATGG CACCACCGAT CCCCACTGTG CCAGCTGGGA CTACTCCAGA 
GCAGATACCA ACTCGGGGGA CTGGAACACT GAGAGCTGCC AGACCTTGGA GACCCAGGCG 
GCTCACACCC GCTGCCAGTG CCAGCACCTG TCCACCTTTG CCGTCCTGGC CCAGCCACCC 
AAGGACCTGA CCCTGGAGCT GGCAGGTGCT CCCTCTGTCC CCCTGGTGAT CGGCTGTGCA 
GTGTCCTGCA TGGCTCTGCT CACCCTGCTG GCCATCTATG CAGCCTTCTG GAGGTTTATA 
AAATCAGAAC GCTCCATCAT CTTGCTGAAC TTCTGCCTGT CCATCCTGGC TTCCAACATT 
CTGATCCTGG TGGGCCAGTC CCGGGTGCTG AGCAAGGGCG TATGCACCAT GACGGCTGCC 
TTCCTACACT TCTTCTTTCT GTCCTCCTTT TGCTGGGTGC TTACAGAGGC CTGGCAATCC 
TATCTGGCTG TCATCGGGCG GATGCGCACC CGCCTGGTTC GCAAGCGCTT CCTCTGCCTG 
GGCTGGGGTC TACCTGCCCT GGTGGTGGCT GTGTCTGTTG GCTTTACTCG CACCAAAGGA 
TATGGTACAT CCAGCTACTG CTGGCTGTCC CTAGAGGGCG GCCTGCTCTA TGCCTTCGTG 
GGTCCAGCAG CAGTCATTGT CCTGGTGAAC ATGCTCATCG GGATTATCGT CTTCAACAAG 
CTCATGGCTC GCGATGGCGT CTCAGACAAA TCTAAGAAGC AGAGGGCTGG GTCGGAGCGG 
TGCCCCTGGG CCAGCCTGCT CCTTCCCTGC TCAGCGTGTG GAGCGGTCCC CAGCCCCCTG 
CTCAGCTCAG CCTCGGCCAG GAATGCCATC CGACTGTCTT GGAATCTCTG GGGATATGGC 
TCTCAACTGT GTCTTTTTCC AAAGCTTCCA AGGGCTTCAC TCTGGAGTTC CTGCGTGGTA 
CTGCCTCTCC TGGCGCTTAC CTGGATGTCT GCCGTCCTGG CCATGACAGA TCGCCGCTCC 
GTCCTCTTCC AGGCACTCTT TGCCGTTTTC AACTCTGCAC AAGGCTTTGT CATCACCGCT 
GTGCACTGCT TCCTGCGCCG AGAGGTCCAG GATGTGGTAA AGTGTCAGAT GGGTGTGTGT 
CGGGCTGATG AGAGTGAAGA CTCCCCAGAC TCGTGCAAGA ACGGGCAGCT GCAGATCCTG 
TCAGACTTTG AAAAGGACGT GGATCTGGCT TGTCAGACAG TTCTGTTCAA GGAGGTCAAC 
ACCTGCAACC CGTCCACCAT TACCGGCACT CTGTCCCGCC TGTCTCTGGA TGAGGATGAG 
GAGCCCAAGT CCTGTCTCGT GGGTCCTGAG GGTGGCCTCA GCTTCTCACC ACTGCCTGGG 
AACATCCTGG TACCCATGGC AGCCTCACCA GGTCTAGGGG AGCCACCACC ACCCCAGGAG 
ACCAACCCTG TGTACATGTG TGGGGAGGGT GGCCTGCGGC AGTTGGACCT TACATGGATA 
CGGCAGAGTG AACCAGGCTC TGAGGGGGAC TACATGGTTC TGCCCCGGCG GACTTTGAGC 
CTGCAGCCTG GTGGTGGGGG TACAGCGGGT GAGGAGGCCC CAAGGGCCCG GCCTGAGGGG 
ACCCCCCGGC GGGCTGCCAA AACGGTAGCC CACACTGAAG GCTACCCCAG CTTCCTGTCT 
GTGGAGCACT CGGGTCTAGG GCTGGGCCCT GCCTATGGGT CTCTCCAGAA CCCGTATGGA 
ATGACCTTCC AACCACCACC ACCAACACCC AGCGCCCGCC AAGTACCAGA GCCAGGAGAA 
CGTAGCCGGA CCATGCCCCG TACAGTGCCT GGTTCCACCA TGAAGCTGGG CTCCCTTGAG 
CGAAAGAAGC TTCGGTATTC GGACTTGGAC TTTGAGAAGG TGATGCACAC TCGGAAACGG 
CACTCGGAAC TCTACCACGA ACTCAACCAG AAGTTCCACA CTTTCGACCG CTACCGTAGC 
CAGTCCTCAG CCAAGGAGAA ACCCAGCCCC CCCGGGGGAC GCCCTGGCTT GTCCCAGCAC 
AGGAGGCATC AAAGCTGGAG CACCTTCAAA TCTATGACAC TGGGCTCACT GCCCCCCAAG 
CCCCGAGAAC GGCTGGCCCT GCACCGGACA GCAGCCTGGG AGCCCACAGA ACCGCCAGAC 
GGCGACTTCC AGACAGAGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adgrb2 ( XM_030253456.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030109316.1 Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant X6, mRNA.

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CloneID	OMu73518
Clone ID Related Accession (Same CDS sequence)	XM_030253456.1 , XM_011240511.1
Accession Version	XM_030253456.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4584bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	adhesion G protein-coupled receptor B2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCAG CCTGTCCCCT CTTACTGTCT GTGATTCTGT CCCTGCGCCT GGCCACGGCC 
TTCGACCCTG CCCCCAGTGC CTGCTCTGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTC 
TCGCTGCAGG ACCTCTTTCC CACCATCGCC TCGGGCTGCT CCTGGACCCT GGAGAACCCA 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGGC AGGAGCAGGT TTGCACACAC 
TTTGCCCCGC GCCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTTACCTG CCTGCGGCCT 
GGTCCAGAGG AAGCCACAGC CCGGGCTGAG TCGGAGGTGG GACGGCCAGA GGAGGAGGAG 
GAGGAGGCGG CGGCAGCAGC ATCAGGGTTG GAGTTGTGTG GTGGCTCAGG CCCCTTTACC 
TTTCTGCACT TCGACAAGAA CTTTGTGCAG CTGTGCCTGT CGGCTGAGCC CTCTGAGGCC 
CCTCGTCTGC TAGCGCCTGC TGCCCTGGCC TTCCGTTTTG TGGAGGTCTT GCTGATCAAC 
AACAACAACT CCAGCCAGTT CACCTGTGGT GTGCTCTGCC GCTGGAGTGA GGAGTGTGGC 
CGGGCTGCAG GCAGGGCTTG TGGCTTTGCA CAGCCAGGGT GTAGTTGTCC TGGGGAGGCA 
GGGGCCAACC CCGCCACCAC CACATCTCCG GGGCCTCCGG TTGCCCACAC CCTGTCCAAT 
GCCCTGGTGC CCGGGGGCCC AGCCCCTCCT GCTGAGGCCG ACTTGCACTC GGGGAGCAGC 
AATGACCTGT TCACCACCGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG 
AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTAT ACATGGCGCA GACAGTGCAC 
GGCGTGTGGG AGGAGTGGGG GTCCTGGAGC CTGTGCTCCC GCAGCTGCGG GCGGGGGTCT 
CGGAGCCGGA TGCGGACCTG CGTGCCCCCC CAGCACGGCG GCAAGGCCTG CGAGGGTCCC 
GAGCTGCAGA CTAAACTCTG CAGTATGGCC GCCTGCCCGG TGGAAGGCCA GTGGCTAGAA 
TGGGGTCCCT GGGGCCCATG CTCATCATCT TGTGCCAATG GGACCCAGCA GCGCAGCCGG 
AAATGCAGTG TGGCGGGTCC AGCCTGGGCC ACGTGCGCAG GTGCCCTCAC GGATACCCGT 
GAGTGCAGCA ATCTCGATTG CCCGGCCACT GACGGCAAGT GGGGGCCGTG GAACGCGTGG 
AGCCTGTGCT CCAAGACGTG TGACACGGGC TGGCAACGCC GCTTCCGCAT GTGCCAGGCT 
TCTGGCACAC AGGGCTACCC TTGCGAGGGC ACAGGAGAGG AGGTGAAACC CTGCAGTGAG 
AAGAGGTGTC CAGCCTTCCA TGAGATGTGC AGGGATGAGT ACGTGATGTT GATGACATGG 
AAGAGGGCGG CAGCTGGCGA GATCATTTAC AACAAGTGTC CCCCTAATGC CTCGGGTTCT 
GCTAGCCGCC GCTGTCTCCT CAGTGCCCAG GGCGTAGCAT ACTGGGGACT GCCCAGCTTT 
GCTCGTTGCA TATCCCATGA ATACCGCTAC CTGTACCTGT CACTTCGGGA ACACCTGGCT 
AAGGGCCAGC GCATGCTGGC AGGTGAGGGC ATGTCACAGG TGGTGCGGAG CCTGCAGGAG 
CTACTGGCAC GGCGCACTTA CTACAGCGGG GACCTGCTCT TCTCTGTGGA CATCCTAAGG 
AACGTCACTG ACACCTTCAA GAGGGCCACC TATGTCCCTT CCGCCGATGA CGTGCAGCGT 
TTCTTCCAGG TGGTGAGCTT CATGGTGGAT TCAGAAAACA AGGACAAATG GGATGATGCT 
CAGCAGGTGT CACCGGGCTC TGTGCACCTG CTGCGTGTTG TGGAAGATTT CATTCACCTC 
GTGGGCGACG CTCTCAAGGC CTTCCAGAGC TCTCTCATTG TCACGGACAA TCTGGTGATC 
AGCATTCAGA GAGAGCCTAT CTCCGCCGTG TCCAGTGACA TCACGTTTCC CATGCGGGGC 
CGCAGGGGCA TGAAGGACTG GGTGCGACAC TCAGAGGATC GTCTCTTTCT ACCCAAGGAG 
GTGCTCAGCC TGTCCTCCCC AGGAAAGCCA GCCACACCTG GGGCAGCCAC AGCAGGCAGC 
CCGGGCAGGG GGAGGGGCCC AGGAACGGTG CCCCCTGGCC CAGGCCACGC CCACCAGCGC 
CTTCTCCCAG CTGACCCCGA AGAGTCCTCC TCCTACTTTG TGATCGGTGC TGTGCTCTAC 
CGCACCCTTG GCCTCATCCT GCCGCCCCCC AGGCCTCCAC TTGCTGTCAC CTCCCGGGTG 
ATGACAGTGA CTGTGCGTCC CCCCACCCAG CCTCCAGCTG AGCCCCTCAT TACAGTGGAA 
CTCTCGTACA TCATCAATGG CACCACCGAT CCCCACTGTG CCAGCTGGGA CTACTCCAGA 
GCAGATACCA ACTCGGGGGA CTGGAACACT GAGAGCTGCC AGACCTTGGA GACCCAGGCG 
GCTCACACCC GCTGCCAGTG CCAGCACCTG TCCACCTTTG CCGTCCTGGC CCAGCCACCC 
AAGGACCTGA CCCTGGAGCT GGCAGGTGCT CCCTCTGTCC CCCTGGTGAT CGGCTGTGCA 
GTGTCCTGCA TGGCTCTGCT CACCCTGCTG GCCATCTATG CAGCCTTCTG GAGGTTTATA 
AAATCAGAAC GCTCCATCAT CTTGCTGAAC TTCTGCCTGT CCATCCTGGC TTCCAACATT 
CTGATCCTGG TGGGCCAGTC CCGGGTGCTG AGCAAGGGCG TATGCACCAT GACGGCTGCC 
TTCCTACACT TCTTCTTTCT GTCCTCCTTT TGCTGGGTGC TTACAGAGGC CTGGCAATCC 
TATCTGGCTG TCATCGGGCG GATGCGCACC CGCCTGGTTC GCAAGCGCTT CCTCTGCCTG 
GGCTGGGGTC TACCTGCCCT GGTGGTGGCT GTGTCTGTTG GCTTTACTCG CACCAAAGGA 
TATGGTACAT CCAGCTACTG CTGGCTGTCC CTAGAGGGCG GCCTGCTCTA TGCCTTCGTG 
GGTCCAGCAG CAGTCATTGT CCTGGTGAAC ATGCTCATCG GGATTATCGT CTTCAACAAG 
CTCATGGCTC GCGATGGCGT CTCAGACAAA TCTAAGAAGC AGAGGGCTGG GTCGGAGCGG 
TGCCCCTGGG CCAGCCTGCT CCTTCCCTGC TCAGCGTGTG GAGCGGTCCC CAGCCCCCTG 
CTCAGCTCAG CCTCGGCCAG GAATGCCATC CGACTGTCTT GGAATCTCTG GGGATATGGC 
TCTCAACTGT GTCTTTTTCC AAAGCTTCCA AGGGCTTCAC TCTGGAGTTC CTGCGTGGTA 
CTGCCTCTCC TGGCGCTTAC CTGGATGTCT GCCGTCCTGG CCATGACAGA TCGCCGCTCC 
GTCCTCTTCC AGGCACTCTT TGCCGTTTTC AACTCTGCAC AAGGCTTTGT CATCACCGCT 
GTGCACTGCT TCCTGCGCCG AGAGGTCCAG GATGTGGTAA AGTGTCAGAT GGGTGTGTGT 
CGGGCTGATG AGAGTGAAGA CTCCCCAGAC TCGTGCAAGA ACGGGCAGCT GCAGATCCTG 
TCAGACTTTG AAAAGGACGT GGATCTGGCT TGTCAGACAG TTCTGTTCAA GGAGGTCAAC 
ACCTGCAACC CGTCCACCAT TACCGGCACT CTGTCCCGCC TGTCTCTGGA TGAGGATGAG 
GAGCCCAAGT CCTGTCTCGT GGGTCCTGAG GGTGGCCTCA GCTTCTCACC ACTGCCTGGG 
AACATCCTGG TACCCATGGC AGCCTCACCA GGTCTAGGGG AGCCACCACC ACCCCAGGAG 
ACCAACCCTG TGTACATGTG TGGGGAGGGT GGCCTGCGGC AGTTGGACCT TACATGGATA 
CGGCAGAGTG AACCAGGCTC TGAGGGGGAC TACATGGTTC TGCCCCGGCG GACTTTGAGC 
CTGCAGCCTG GTGGTGGGGG TACAGCGGGT GAGGAGGCCC CAAGGGCCCG GCCTGAGGGG 
ACCCCCCGGC GGGCTGCCAA AACGGTAGCC CACACTGAAG GCTACCCCAG CTTCCTGTCT 
GTGGAGCACT CGGGTCTAGG GCTGGGCCCT GCCTATGGGT CTCTCCAGAA CCCGTATGGA 
ATGACCTTCC AACCACCACC ACCAACACCC AGCGCCCGCC AAGTACCAGA GCCAGGAGAA 
CGTAGCCGGA CCATGCCCCG TACAGTGCCT GGTTCCACCA TGAAGCTGGG CTCCCTTGAG 
CGAAAGAAGC TTCGGTATTC GGACTTGGAC TTTGAGAAGG TGATGCACAC TCGGAAACGG 
CACTCGGAAC TCTACCACGA ACTCAACCAG AAGTTCCACA CTTTCGACCG CTACCGTAGC 
CAGTCCTCAG CCAAGGAGAA ACCCAGCCCC CCCGGGGGAC GCCCTGGCTT GTCCCAGCAC 
AGGAGGCATC AAAGCTGGAG CACCTTCAAA TCTATGACAC TGGGCTCACT GCCCCCCAAG 
CCCCGAGAAC GGCTGGCCCT GCACCGGACA GCAGCCTGGG AGCCCACAGA ACCGCCAGAC 
GGCGACTTCC AGACAGAGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030109316.1
CDS	551..5134
Translation	MTPACPLLLSVILSLRLATAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCTHFAPRLLPLDHYLVNFTCLRPGPEEATARAESEVGRPEEEEEEAAAAASGLELCGGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGANPATTTSPGPPVAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSSSCANGTQQRSRKCSVAGPAWATCAGALTDTRECSNLDCPATDGKWGPWNAWSLCSKTCDTGWQRRFRMCQASGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKRAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDSENKDKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPISAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSSPGKPATPGAATAGSPGRGRGPGTVPPGPGHAHQRLLPADPEESSSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADTNSGDWNTESCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGAPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNILILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGVSDKSKKQRAGSERCPWASLLLPCSACGAVPSPLLSSASARNAIRLSWNLWGYGSQLCLFPKLPRASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGGLSFSPLPGNILVPMAASPGLGEPPPPQETNPVYMCGEGGLRQLDLTWIRQSEPGSEGDYMVLPRRTLSLQPGGGGTAGEEAPRARPEGTPRRAAKTVAHTEGYPSFLSVEHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVPEPGERSRTMPRTVPGSTMKLGSLERKKLRYSDLDFEKVMHTRKRHSELYHELNQKFHTFDRYRSQSSAKEKPSPPGGRPGLSQHRRHQSWSTFKSMTLGSLPPKPRERLALHRTAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_030253456.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030253456.1

ORF Insert Sequence:

ATGACCCCAG CCTGTCCCCT CTTACTGTCT GTGATTCTGT CCCTGCGCCT GGCCACGGCC 
TTCGACCCTG CCCCCAGTGC CTGCTCTGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTC 
TCGCTGCAGG ACCTCTTTCC CACCATCGCC TCGGGCTGCT CCTGGACCCT GGAGAACCCA 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGGC AGGAGCAGGT TTGCACACAC 
TTTGCCCCGC GCCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTTACCTG CCTGCGGCCT 
GGTCCAGAGG AAGCCACAGC CCGGGCTGAG TCGGAGGTGG GACGGCCAGA GGAGGAGGAG 
GAGGAGGCGG CGGCAGCAGC ATCAGGGTTG GAGTTGTGTG GTGGCTCAGG CCCCTTTACC 
TTTCTGCACT TCGACAAGAA CTTTGTGCAG CTGTGCCTGT CGGCTGAGCC CTCTGAGGCC 
CCTCGTCTGC TAGCGCCTGC TGCCCTGGCC TTCCGTTTTG TGGAGGTCTT GCTGATCAAC 
AACAACAACT CCAGCCAGTT CACCTGTGGT GTGCTCTGCC GCTGGAGTGA GGAGTGTGGC 
CGGGCTGCAG GCAGGGCTTG TGGCTTTGCA CAGCCAGGGT GTAGTTGTCC TGGGGAGGCA 
GGGGCCAACC CCGCCACCAC CACATCTCCG GGGCCTCCGG TTGCCCACAC CCTGTCCAAT 
GCCCTGGTGC CCGGGGGCCC AGCCCCTCCT GCTGAGGCCG ACTTGCACTC GGGGAGCAGC 
AATGACCTGT TCACCACCGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG 
AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTAT ACATGGCGCA GACAGTGCAC 
GGCGTGTGGG AGGAGTGGGG GTCCTGGAGC CTGTGCTCCC GCAGCTGCGG GCGGGGGTCT 
CGGAGCCGGA TGCGGACCTG CGTGCCCCCC CAGCACGGCG GCAAGGCCTG CGAGGGTCCC 
GAGCTGCAGA CTAAACTCTG CAGTATGGCC GCCTGCCCGG TGGAAGGCCA GTGGCTAGAA 
TGGGGTCCCT GGGGCCCATG CTCATCATCT TGTGCCAATG GGACCCAGCA GCGCAGCCGG 
AAATGCAGTG TGGCGGGTCC AGCCTGGGCC ACGTGCGCAG GTGCCCTCAC GGATACCCGT 
GAGTGCAGCA ATCTCGATTG CCCGGCCACT GACGGCAAGT GGGGGCCGTG GAACGCGTGG 
AGCCTGTGCT CCAAGACGTG TGACACGGGC TGGCAACGCC GCTTCCGCAT GTGCCAGGCT 
TCTGGCACAC AGGGCTACCC TTGCGAGGGC ACAGGAGAGG AGGTGAAACC CTGCAGTGAG 
AAGAGGTGTC CAGCCTTCCA TGAGATGTGC AGGGATGAGT ACGTGATGTT GATGACATGG 
AAGAGGGCGG CAGCTGGCGA GATCATTTAC AACAAGTGTC CCCCTAATGC CTCGGGTTCT 
GCTAGCCGCC GCTGTCTCCT CAGTGCCCAG GGCGTAGCAT ACTGGGGACT GCCCAGCTTT 
GCTCGTTGCA TATCCCATGA ATACCGCTAC CTGTACCTGT CACTTCGGGA ACACCTGGCT 
AAGGGCCAGC GCATGCTGGC AGGTGAGGGC ATGTCACAGG TGGTGCGGAG CCTGCAGGAG 
CTACTGGCAC GGCGCACTTA CTACAGCGGG GACCTGCTCT TCTCTGTGGA CATCCTAAGG 
AACGTCACTG ACACCTTCAA GAGGGCCACC TATGTCCCTT CCGCCGATGA CGTGCAGCGT 
TTCTTCCAGG TGGTGAGCTT CATGGTGGAT TCAGAAAACA AGGACAAATG GGATGATGCT 
CAGCAGGTGT CACCGGGCTC TGTGCACCTG CTGCGTGTTG TGGAAGATTT CATTCACCTC 
GTGGGCGACG CTCTCAAGGC CTTCCAGAGC TCTCTCATTG TCACGGACAA TCTGGTGATC 
AGCATTCAGA GAGAGCCTAT CTCCGCCGTG TCCAGTGACA TCACGTTTCC CATGCGGGGC 
CGCAGGGGCA TGAAGGACTG GGTGCGACAC TCAGAGGATC GTCTCTTTCT ACCCAAGGAG 
GTGCTCAGCC TGTCCTCCCC AGGAAAGCCA GCCACACCTG GGGCAGCCAC AGCAGGCAGC 
CCGGGCAGGG GGAGGGGCCC AGGAACGGTG CCCCCTGGCC CAGGCCACGC CCACCAGCGC 
CTTCTCCCAG CTGACCCCGA AGAGTCCTCC TCCTACTTTG TGATCGGTGC TGTGCTCTAC 
CGCACCCTTG GCCTCATCCT GCCGCCCCCC AGGCCTCCAC TTGCTGTCAC CTCCCGGGTG 
ATGACAGTGA CTGTGCGTCC CCCCACCCAG CCTCCAGCTG AGCCCCTCAT TACAGTGGAA 
CTCTCGTACA TCATCAATGG CACCACCGAT CCCCACTGTG CCAGCTGGGA CTACTCCAGA 
GCAGATACCA ACTCGGGGGA CTGGAACACT GAGAGCTGCC AGACCTTGGA GACCCAGGCG 
GCTCACACCC GCTGCCAGTG CCAGCACCTG TCCACCTTTG CCGTCCTGGC CCAGCCACCC 
AAGGACCTGA CCCTGGAGCT GGCAGGTGCT CCCTCTGTCC CCCTGGTGAT CGGCTGTGCA 
GTGTCCTGCA TGGCTCTGCT CACCCTGCTG GCCATCTATG CAGCCTTCTG GAGGTTTATA 
AAATCAGAAC GCTCCATCAT CTTGCTGAAC TTCTGCCTGT CCATCCTGGC TTCCAACATT 
CTGATCCTGG TGGGCCAGTC CCGGGTGCTG AGCAAGGGCG TATGCACCAT GACGGCTGCC 
TTCCTACACT TCTTCTTTCT GTCCTCCTTT TGCTGGGTGC TTACAGAGGC CTGGCAATCC 
TATCTGGCTG TCATCGGGCG GATGCGCACC CGCCTGGTTC GCAAGCGCTT CCTCTGCCTG 
GGCTGGGGTC TACCTGCCCT GGTGGTGGCT GTGTCTGTTG GCTTTACTCG CACCAAAGGA 
TATGGTACAT CCAGCTACTG CTGGCTGTCC CTAGAGGGCG GCCTGCTCTA TGCCTTCGTG 
GGTCCAGCAG CAGTCATTGT CCTGGTGAAC ATGCTCATCG GGATTATCGT CTTCAACAAG 
CTCATGGCTC GCGATGGCGT CTCAGACAAA TCTAAGAAGC AGAGGGCTGG GTCGGAGCGG 
TGCCCCTGGG CCAGCCTGCT CCTTCCCTGC TCAGCGTGTG GAGCGGTCCC CAGCCCCCTG 
CTCAGCTCAG CCTCGGCCAG GAATGCCATC CGACTGTCTT GGAATCTCTG GGGATATGGC 
TCTCAACTGT GTCTTTTTCC AAAGCTTCCA AGGGCTTCAC TCTGGAGTTC CTGCGTGGTA 
CTGCCTCTCC TGGCGCTTAC CTGGATGTCT GCCGTCCTGG CCATGACAGA TCGCCGCTCC 
GTCCTCTTCC AGGCACTCTT TGCCGTTTTC AACTCTGCAC AAGGCTTTGT CATCACCGCT 
GTGCACTGCT TCCTGCGCCG AGAGGTCCAG GATGTGGTAA AGTGTCAGAT GGGTGTGTGT 
CGGGCTGATG AGAGTGAAGA CTCCCCAGAC TCGTGCAAGA ACGGGCAGCT GCAGATCCTG 
TCAGACTTTG AAAAGGACGT GGATCTGGCT TGTCAGACAG TTCTGTTCAA GGAGGTCAAC 
ACCTGCAACC CGTCCACCAT TACCGGCACT CTGTCCCGCC TGTCTCTGGA TGAGGATGAG 
GAGCCCAAGT CCTGTCTCGT GGGTCCTGAG GGTGGCCTCA GCTTCTCACC ACTGCCTGGG 
AACATCCTGG TACCCATGGC AGCCTCACCA GGTCTAGGGG AGCCACCACC ACCCCAGGAG 
ACCAACCCTG TGTACATGTG TGGGGAGGGT GGCCTGCGGC AGTTGGACCT TACATGGATA 
CGGCAGAGTG AACCAGGCTC TGAGGGGGAC TACATGGTTC TGCCCCGGCG GACTTTGAGC 
CTGCAGCCTG GTGGTGGGGG TACAGCGGGT GAGGAGGCCC CAAGGGCCCG GCCTGAGGGG 
ACCCCCCGGC GGGCTGCCAA AACGGTAGCC CACACTGAAG GCTACCCCAG CTTCCTGTCT 
GTGGAGCACT CGGGTCTAGG GCTGGGCCCT GCCTATGGGT CTCTCCAGAA CCCGTATGGA 
ATGACCTTCC AACCACCACC ACCAACACCC AGCGCCCGCC AAGTACCAGA GCCAGGAGAA 
CGTAGCCGGA CCATGCCCCG TACAGTGCCT GGTTCCACCA TGAAGCTGGG CTCCCTTGAG 
CGAAAGAAGC TTCGGTATTC GGACTTGGAC TTTGAGAAGG TGATGCACAC TCGGAAACGG 
CACTCGGAAC TCTACCACGA ACTCAACCAG AAGTTCCACA CTTTCGACCG CTACCGTAGC 
CAGTCCTCAG CCAAGGAGAA ACCCAGCCCC CCCGGGGGAC GCCCTGGCTT GTCCCAGCAC 
AGGAGGCATC AAAGCTGGAG CACCTTCAAA TCTATGACAC TGGGCTCACT GCCCCCCAAG 
CCCCGAGAAC GGCTGGCCCT GCACCGGACA GCAGCCTGGG AGCCCACAGA ACCGCCAGAC 
GGCGACTTCC AGACAGAGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adgrb2 ( NM_001199696.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001186625.1 Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant 2, mRNA.

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CloneID	OMu14259
Clone ID Related Accession (Same CDS sequence)	NM_001199696.1 , NM_001199696.2
Accession Version	NM_001199696.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4521bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Mus musculus(house mouse)
Product	adhesion G protein-coupled receptor B2 isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ162521.1, AK158735.1 and BC056926.1. Transcript Variant: This variant (2) has several differences in the coding region, compared to variant 1. The resulting protein (isoform 2) is shorter when it is compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK158735.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849379 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCCCAG CCTGTCCCCT CTTACTGTCT GTGATTCTGT CCCTGCGCCT GGCCACGGCC 
TTCGACCCTG CCCCCAGTGC CTGCTCTGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTC 
TCGCTGCAGG ACCTCTTTCC CACCATCGCC TCGGGCTGCT CCTGGACCCT GGAGAACCCA 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGGC AGGAGCAGGT TTGCACACAC 
TTTGCCCCGC GCCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTTACCTG CCTGCGGCCT 
GGTCCAGAGG AAGCCACAGC CCGGGCTGAG TCGGAGGTGG GACGGCCAGA GGAGGAGGAG 
GAGGAGGCGG CGGCAGCAGC ATCAGGGTTG GAGTTGTGTG GTGGCTCAGG CCCCTTTACC 
TTTCTGCACT TCGACAAGAA CTTTGTGCAG CTGTGCCTGT CGGCTGAGCC CTCTGAGGCC 
CCTCGTCTGC TAGCGCCTGC TGCCCTGGCC TTCCGTTTTG TGGAGGTCTT GCTGATCAAC 
AACAACAACT CCAGCCAGTT CACCTGTGGT GTGCTCTGCC GCTGGAGTGA GGAGTGTGGC 
CGGGCTGCAG GCAGGGCTTG TGGCTTTGCA CAGCCAGGGT GTAGTTGTCC TGGGGAGGCA 
GGGGCCAACC CCGCCACCAC CACATCTCCG GGGCCTCCGG TTGCCCACAC CCTGTCCAAT 
GCCCTGGTGC CCGGGGGCCC AGCCCCTCCT GCTGAGGCCG ACTTGCACTC GGGGAGCAGC 
AATGACCTGT TCACCACCGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG 
AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTAT ACATGGCGCA GACAGTGCAC 
GGCGTGTGGG AGGAGTGGGG GTCCTGGAGC CTGTGCTCCC GCAGCTGCGG GCGGGGGTCT 
CGGAGCCGGA TGCGGACCTG CGTGCCCCCC CAGCACGGCG GCAAGGCCTG CGAGGGTCCC 
GAGCTGCAGA CTAAACTCTG CAGTATGGCC GCCTGCCCGG TGGAAGGCCA GTGGCTAGAA 
TGGGGTCCCT GGGGCCCATG CTCATCATCT TGTGCCAATG GGACCCAGCA GCGCAGCCGG 
AAATGCAGTG TGGCGGGTCC AGCCTGGGCC ACGTGCGCAG GTGCCCTCAC GGATACCCGT 
GAGTGCAGCA ATCTCGATTG CCCGGCCACT GACGGCAAGT GGGGGCCGTG GAACGCGTGG 
AGCCTGTGCT CCAAGACGTG TGACACGGGC TGGCAACGCC GCTTCCGCAT GTGCCAGGCT 
TCTGGCACAC AGGGCTACCC TTGCGAGGGC ACAGGAGAGG AGGTGAAACC CTGCAGTGAG 
AAGAGGTGTC CAGCCTTCCA TGAGATGTGC AGGGATGAGT ACGTGATGTT GATGACATGG 
AAGAGGGCGG CAGCTGGCGA GATCATTTAC AACAAGTGTC CCCCTAATGC CTCGGGTTCT 
GCTAGCCGCC GCTGTCTCCT CAGTGCCCAG GGCGTAGCAT ACTGGGGACT GCCCAGCTTT 
GCTCGTTGCA TATCCCATGA ATACCGCTAC CTGTACCTGT CACTTCGGGA ACACCTGGCT 
AAGGGCCAGC GCATGCTGGC AGGTGAGGGC ATGTCACAGG TGGTGCGGAG CCTGCAGGAG 
CTACTGGCAC GGCGCACTTA CTACAGCGGG GACCTGCTCT TCTCTGTGGA CATCCTAAGG 
AACGTCACTG ACACCTTCAA GAGGGCCACC TATGTCCCTT CCGCCGATGA CGTGCAGCGT 
TTCTTCCAGG TGGTGAGCTT CATGGTGGAT TCAGAAAACA AGGACAAATG GGATGATGCT 
CAGCAGGTGT CACCGGGCTC TGTGCACCTG CTGCGTGTTG TGGAAGATTT CATTCACCTC 
GTGGGCGACG CTCTCAAGGC CTTCCAGAGC TCTCTCATTG TCACGGACAA TCTGGTGATC 
AGCATTCAGA GAGAGCCTAT CTCCGCCGTG TCCAGTGACA TCACGTTTCC CATGCGGGGC 
CGCAGGGGCA TGAAGGACTG GGTGCGACAC TCAGAGGATC GTCTCTTTCT ACCCAAGGAG 
GTGCTCAGCC TGTCCTCCCC AGGAAAGCCA GCCACACCTG GGGCAGCCAC AGCAGGCAGC 
CCGGGCAGGG GGAGGGGCCC AGGAACGGTG CCCCCTGGCC CAGGCCACGC CCACCAGCGC 
CTTCTCCCAG CTGACCCCGA AGAGTCCTCC TCCTACTTTG TGATCGGTGC TGTGCTCTAC 
CGCACCCTTG GCCTCATCCT GCCGCCCCCC AGGCCTCCAC TTGCTGTCAC CTCCCGGGTG 
ATGACAGTGA CTGTGCGTCC CCCCACCCAG CCTCCAGCTG AGCCCCTCAT TACAGTGGAA 
CTCTCGTACA TCATCAATGG CACCACCGAT CCCCACTGTG CCAGCTGGGA CTACTCCAGA 
GCAGATACCA ACTCGGGGGA CTGGAACACT GAGAGCTGCC AGACCTTGGA GACCCAGGCG 
GCTCACACCC GCTGCCAGTG CCAGCACCTG TCCACCTTTG CCGTCCTGGC CCAGCCACCC 
AAGGACCTGA CCCTGGAGCT GGCAGGTGCT CCCTCTGTCC CCCTGGTGAT CGGCTGTGCA 
GTGTCCTGCA TGGCTCTGCT CACCCTGCTG GCCATCTATG CAGCCTTCTG GAGGTTTATA 
AAATCAGAAC GCTCCATCAT CTTGCTGAAC TTCTGCCTGT CCATCCTGGC TTCCAACATT 
CTGATCCTGG TGGGCCAGTC CCGGGTGCTG AGCAAGGGCG TATGCACCAT GACGGCTGCC 
TTCCTACACT TCTTCTTTCT GTCCTCCTTT TGCTGGGTGC TTACAGAGGC CTGGCAATCC 
TATCTGGCTG TCATCGGGCG GATGCGCACC CGCCTGGTTC GCAAGCGCTT CCTCTGCCTG 
GGCTGGGGTC TACCTGCCCT GGTGGTGGCT GTGTCTGTTG GCTTTACTCG CACCAAAGGA 
TATGGTACAT CCAGCTACTG CTGGCTGTCC CTAGAGGGCG GCCTGCTCTA TGCCTTCGTG 
GGTCCAGCAG CAGTCATTGT CCTGGTGAAC ATGCTCATCG GGATTATCGT CTTCAACAAG 
CTCATGGCTC GCGATGGCGT CTCAGACAAA TCTAAGAAGC AGAGGGCTGG GTCGGAGCGG 
TGCCCCTGGG CCAGCCTGCT CCTTCCCTGC TCAGCGTGTG GAGCGGTCCC CAGCCCCCTG 
CTCAGCTCAG CCTCGGCCAG GAATGCCATG GCTTCACTCT GGAGTTCCTG CGTGGTACTG 
CCTCTCCTGG CGCTTACCTG GATGTCTGCC GTCCTGGCCA TGACAGATCG CCGCTCCGTC 
CTCTTCCAGG CACTCTTTGC CGTTTTCAAC TCTGCACAAG GCTTTGTCAT CACCGCTGTG 
CACTGCTTCC TGCGCCGAGA GGTCCAGGAT GTGGTAAAGT GTCAGATGGG TGTGTGTCGG 
GCTGATGAGA GTGAAGACTC CCCAGACTCG TGCAAGAACG GGCAGCTGCA GATCCTGTCA 
GACTTTGAAA AGGACGTGGA TCTGGCTTGT CAGACAGTTC TGTTCAAGGA GGTCAACACC 
TGCAACCCGT CCACCATTAC CGGCACTCTG TCCCGCCTGT CTCTGGATGA GGATGAGGAG 
CCCAAGTCCT GTCTCGTGGG TCCTGAGGGT GGCCTCAGCT TCTCACCACT GCCTGGGAAC 
ATCCTGGTAC CCATGGCAGC CTCACCAGGT CTAGGGGAGC CACCACCACC CCAGGAGACC 
AACCCTGTGT ACATGTGTGG GGAGGGTGGC CTGCGGCAGT TGGACCTTAC ATGGATACGG 
CAGAGTGAAC CAGGCTCTGA GGGGGACTAC ATGGTTCTGC CCCGGCGGAC TTTGAGCCTG 
CAGCCTGGTG GTGGGGGTAC AGCGGGTGAG GAGGCCCCAA GGGCCCGGCC TGAGGGGACC 
CCCCGGCGGG CTGCCAAAAC GGTAGCCCAC ACTGAAGGCT ACCCCAGCTT CCTGTCTGTG 
GAGCACTCGG GTCTAGGGCT GGGCCCTGCC TATGGGTCTC TCCAGAACCC GTATGGAATG 
ACCTTCCAAC CACCACCACC AACACCCAGC GCCCGCCAAG TACCAGAGCC AGGAGAACGT 
AGCCGGACCA TGCCCCGTAC AGTGCCTGGT TCCACCATGA AGCTGGGCTC CCTTGAGCGA 
AAGAAGCTTC GGTATTCGGA CTTGGACTTT GAGAAGGTGA TGCACACTCG GAAACGGCAC 
TCGGAACTCT ACCACGAACT CAACCAGAAG TTCCACACTT TCGACCGCTA CCGTAGCCAG 
TCCTCAGCCA AGGAGAAACC CAGCCCCCCC GGGGGACGCC CTGGCTTGTC CCAGCACAGG 
AGGCATCAAA GCTGGAGCAC CTTCAAATCT ATGACACTGG GCTCACTGCC CCCCAAGCCC 
CGAGAACGGC TGGCCCTGCA CCGGACAGCA GCCTGGGAGC CCACAGAACC GCCAGACGGC 
GACTTCCAGA CAGAGGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186625.1
CDS	246..4766
Translation	MTPACPLLLSVILSLRLATAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCTHFAPRLLPLDHYLVNFTCLRPGPEEATARAESEVGRPEEEEEEAAAAASGLELCGGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGANPATTTSPGPPVAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSSSCANGTQQRSRKCSVAGPAWATCAGALTDTRECSNLDCPATDGKWGPWNAWSLCSKTCDTGWQRRFRMCQASGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKRAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDSENKDKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPISAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSSPGKPATPGAATAGSPGRGRGPGTVPPGPGHAHQRLLPADPEESSSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADTNSGDWNTESCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGAPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNILILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGVSDKSKKQRAGSERCPWASLLLPCSACGAVPSPLLSSASARNAMASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGGLSFSPLPGNILVPMAASPGLGEPPPPQETNPVYMCGEGGLRQLDLTWIRQSEPGSEGDYMVLPRRTLSLQPGGGGTAGEEAPRARPEGTPRRAAKTVAHTEGYPSFLSVEHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVPEPGERSRTMPRTVPGSTMKLGSLERKKLRYSDLDFEKVMHTRKRHSELYHELNQKFHTFDRYRSQSSAKEKPSPPGGRPGLSQHRRHQSWSTFKSMTLGSLPPKPRERLALHRTAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	NM_001199696.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199696.1

ORF Insert Sequence:

ATGACCCCAG CCTGTCCCCT CTTACTGTCT GTGATTCTGT CCCTGCGCCT GGCCACGGCC 
TTCGACCCTG CCCCCAGTGC CTGCTCTGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTC 
TCGCTGCAGG ACCTCTTTCC CACCATCGCC TCGGGCTGCT CCTGGACCCT GGAGAACCCA 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGGC AGGAGCAGGT TTGCACACAC 
TTTGCCCCGC GCCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTTACCTG CCTGCGGCCT 
GGTCCAGAGG AAGCCACAGC CCGGGCTGAG TCGGAGGTGG GACGGCCAGA GGAGGAGGAG 
GAGGAGGCGG CGGCAGCAGC ATCAGGGTTG GAGTTGTGTG GTGGCTCAGG CCCCTTTACC 
TTTCTGCACT TCGACAAGAA CTTTGTGCAG CTGTGCCTGT CGGCTGAGCC CTCTGAGGCC 
CCTCGTCTGC TAGCGCCTGC TGCCCTGGCC TTCCGTTTTG TGGAGGTCTT GCTGATCAAC 
AACAACAACT CCAGCCAGTT CACCTGTGGT GTGCTCTGCC GCTGGAGTGA GGAGTGTGGC 
CGGGCTGCAG GCAGGGCTTG TGGCTTTGCA CAGCCAGGGT GTAGTTGTCC TGGGGAGGCA 
GGGGCCAACC CCGCCACCAC CACATCTCCG GGGCCTCCGG TTGCCCACAC CCTGTCCAAT 
GCCCTGGTGC CCGGGGGCCC AGCCCCTCCT GCTGAGGCCG ACTTGCACTC GGGGAGCAGC 
AATGACCTGT TCACCACCGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG 
AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTAT ACATGGCGCA GACAGTGCAC 
GGCGTGTGGG AGGAGTGGGG GTCCTGGAGC CTGTGCTCCC GCAGCTGCGG GCGGGGGTCT 
CGGAGCCGGA TGCGGACCTG CGTGCCCCCC CAGCACGGCG GCAAGGCCTG CGAGGGTCCC 
GAGCTGCAGA CTAAACTCTG CAGTATGGCC GCCTGCCCGG TGGAAGGCCA GTGGCTAGAA 
TGGGGTCCCT GGGGCCCATG CTCATCATCT TGTGCCAATG GGACCCAGCA GCGCAGCCGG 
AAATGCAGTG TGGCGGGTCC AGCCTGGGCC ACGTGCGCAG GTGCCCTCAC GGATACCCGT 
GAGTGCAGCA ATCTCGATTG CCCGGCCACT GACGGCAAGT GGGGGCCGTG GAACGCGTGG 
AGCCTGTGCT CCAAGACGTG TGACACGGGC TGGCAACGCC GCTTCCGCAT GTGCCAGGCT 
TCTGGCACAC AGGGCTACCC TTGCGAGGGC ACAGGAGAGG AGGTGAAACC CTGCAGTGAG 
AAGAGGTGTC CAGCCTTCCA TGAGATGTGC AGGGATGAGT ACGTGATGTT GATGACATGG 
AAGAGGGCGG CAGCTGGCGA GATCATTTAC AACAAGTGTC CCCCTAATGC CTCGGGTTCT 
GCTAGCCGCC GCTGTCTCCT CAGTGCCCAG GGCGTAGCAT ACTGGGGACT GCCCAGCTTT 
GCTCGTTGCA TATCCCATGA ATACCGCTAC CTGTACCTGT CACTTCGGGA ACACCTGGCT 
AAGGGCCAGC GCATGCTGGC AGGTGAGGGC ATGTCACAGG TGGTGCGGAG CCTGCAGGAG 
CTACTGGCAC GGCGCACTTA CTACAGCGGG GACCTGCTCT TCTCTGTGGA CATCCTAAGG 
AACGTCACTG ACACCTTCAA GAGGGCCACC TATGTCCCTT CCGCCGATGA CGTGCAGCGT 
TTCTTCCAGG TGGTGAGCTT CATGGTGGAT TCAGAAAACA AGGACAAATG GGATGATGCT 
CAGCAGGTGT CACCGGGCTC TGTGCACCTG CTGCGTGTTG TGGAAGATTT CATTCACCTC 
GTGGGCGACG CTCTCAAGGC CTTCCAGAGC TCTCTCATTG TCACGGACAA TCTGGTGATC 
AGCATTCAGA GAGAGCCTAT CTCCGCCGTG TCCAGTGACA TCACGTTTCC CATGCGGGGC 
CGCAGGGGCA TGAAGGACTG GGTGCGACAC TCAGAGGATC GTCTCTTTCT ACCCAAGGAG 
GTGCTCAGCC TGTCCTCCCC AGGAAAGCCA GCCACACCTG GGGCAGCCAC AGCAGGCAGC 
CCGGGCAGGG GGAGGGGCCC AGGAACGGTG CCCCCTGGCC CAGGCCACGC CCACCAGCGC 
CTTCTCCCAG CTGACCCCGA AGAGTCCTCC TCCTACTTTG TGATCGGTGC TGTGCTCTAC 
CGCACCCTTG GCCTCATCCT GCCGCCCCCC AGGCCTCCAC TTGCTGTCAC CTCCCGGGTG 
ATGACAGTGA CTGTGCGTCC CCCCACCCAG CCTCCAGCTG AGCCCCTCAT TACAGTGGAA 
CTCTCGTACA TCATCAATGG CACCACCGAT CCCCACTGTG CCAGCTGGGA CTACTCCAGA 
GCAGATACCA ACTCGGGGGA CTGGAACACT GAGAGCTGCC AGACCTTGGA GACCCAGGCG 
GCTCACACCC GCTGCCAGTG CCAGCACCTG TCCACCTTTG CCGTCCTGGC CCAGCCACCC 
AAGGACCTGA CCCTGGAGCT GGCAGGTGCT CCCTCTGTCC CCCTGGTGAT CGGCTGTGCA 
GTGTCCTGCA TGGCTCTGCT CACCCTGCTG GCCATCTATG CAGCCTTCTG GAGGTTTATA 
AAATCAGAAC GCTCCATCAT CTTGCTGAAC TTCTGCCTGT CCATCCTGGC TTCCAACATT 
CTGATCCTGG TGGGCCAGTC CCGGGTGCTG AGCAAGGGCG TATGCACCAT GACGGCTGCC 
TTCCTACACT TCTTCTTTCT GTCCTCCTTT TGCTGGGTGC TTACAGAGGC CTGGCAATCC 
TATCTGGCTG TCATCGGGCG GATGCGCACC CGCCTGGTTC GCAAGCGCTT CCTCTGCCTG 
GGCTGGGGTC TACCTGCCCT GGTGGTGGCT GTGTCTGTTG GCTTTACTCG CACCAAAGGA 
TATGGTACAT CCAGCTACTG CTGGCTGTCC CTAGAGGGCG GCCTGCTCTA TGCCTTCGTG 
GGTCCAGCAG CAGTCATTGT CCTGGTGAAC ATGCTCATCG GGATTATCGT CTTCAACAAG 
CTCATGGCTC GCGATGGCGT CTCAGACAAA TCTAAGAAGC AGAGGGCTGG GTCGGAGCGG 
TGCCCCTGGG CCAGCCTGCT CCTTCCCTGC TCAGCGTGTG GAGCGGTCCC CAGCCCCCTG 
CTCAGCTCAG CCTCGGCCAG GAATGCCATG GCTTCACTCT GGAGTTCCTG CGTGGTACTG 
CCTCTCCTGG CGCTTACCTG GATGTCTGCC GTCCTGGCCA TGACAGATCG CCGCTCCGTC 
CTCTTCCAGG CACTCTTTGC CGTTTTCAAC TCTGCACAAG GCTTTGTCAT CACCGCTGTG 
CACTGCTTCC TGCGCCGAGA GGTCCAGGAT GTGGTAAAGT GTCAGATGGG TGTGTGTCGG 
GCTGATGAGA GTGAAGACTC CCCAGACTCG TGCAAGAACG GGCAGCTGCA GATCCTGTCA 
GACTTTGAAA AGGACGTGGA TCTGGCTTGT CAGACAGTTC TGTTCAAGGA GGTCAACACC 
TGCAACCCGT CCACCATTAC CGGCACTCTG TCCCGCCTGT CTCTGGATGA GGATGAGGAG 
CCCAAGTCCT GTCTCGTGGG TCCTGAGGGT GGCCTCAGCT TCTCACCACT GCCTGGGAAC 
ATCCTGGTAC CCATGGCAGC CTCACCAGGT CTAGGGGAGC CACCACCACC CCAGGAGACC 
AACCCTGTGT ACATGTGTGG GGAGGGTGGC CTGCGGCAGT TGGACCTTAC ATGGATACGG 
CAGAGTGAAC CAGGCTCTGA GGGGGACTAC ATGGTTCTGC CCCGGCGGAC TTTGAGCCTG 
CAGCCTGGTG GTGGGGGTAC AGCGGGTGAG GAGGCCCCAA GGGCCCGGCC TGAGGGGACC 
CCCCGGCGGG CTGCCAAAAC GGTAGCCCAC ACTGAAGGCT ACCCCAGCTT CCTGTCTGTG 
GAGCACTCGG GTCTAGGGCT GGGCCCTGCC TATGGGTCTC TCCAGAACCC GTATGGAATG 
ACCTTCCAAC CACCACCACC AACACCCAGC GCCCGCCAAG TACCAGAGCC AGGAGAACGT 
AGCCGGACCA TGCCCCGTAC AGTGCCTGGT TCCACCATGA AGCTGGGCTC CCTTGAGCGA 
AAGAAGCTTC GGTATTCGGA CTTGGACTTT GAGAAGGTGA TGCACACTCG GAAACGGCAC 
TCGGAACTCT ACCACGAACT CAACCAGAAG TTCCACACTT TCGACCGCTA CCGTAGCCAG 
TCCTCAGCCA AGGAGAAACC CAGCCCCCCC GGGGGACGCC CTGGCTTGTC CCAGCACAGG 
AGGCATCAAA GCTGGAGCAC CTTCAAATCT ATGACACTGG GCTCACTGCC CCCCAAGCCC 
CGAGAACGGC TGGCCCTGCA CCGGACAGCA GCCTGGGAGC CCACAGAACC GCCAGACGGC 
GACTTCCAGA CAGAGGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adgrb2 ( NM_001290715.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001277644.1 Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant 3, mRNA.

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CloneID	OMu14294
Clone ID Related Accession (Same CDS sequence)	NM_001290715.1 , NM_001290715.2
Accession Version	NM_001290715.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Mus musculus(house mouse)
Product	adhesion G protein-coupled receptor B2 isoform 3 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK147485.1, BC056926.1 and AK144559.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. It encodes isoform 3, which is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY168407.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849379 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCCCAG CCTGTCCCCT CTTACTGTCT GTGATTCTGT CCCTGCGCCT GGCCACGGCC 
TTCGACCCTG CCCCCAGTGC CTGCTCTGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTC 
TCGCTGCAGG ACCTCTTTCC CACCATCGCC TCGGGCTGCT CCTGGACCCT GGAGAACCCA 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGGC AGGAGCAGGT TTGCACACAC 
TTTGCCCCGC GCCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTTACCTG CCTGCGGCCT 
GGTCCAGAGG AAGCCACAGC CCGGGCTGAG TCGGAGGTGG GACGGCCAGA GGAGGAGGAG 
GAGGAGGCGG CGGCAGCAGC ATCAGGGTTG GAGTTGTGTG GTGGCTCAGG CCCCTTTACC 
TTTCTGCACT TCGACAAGAA CTTTGTGCAG CTGTGCCTGT CGGCTGAGCC CTCTGAGGCC 
CCTCGTCTGC TAGCGCCTGC TGCCCTGGCC TTCCGTTTTG TGGAGGTCTT GCTGATCAAC 
AACAACAACT CCAGCCAGTT CACCTGTGGT GTGCTCTGCC GCTGGAGTGA GGAGTGTGGC 
CGGGCTGCAG GCAGGGCTTG TGGCTTTGCA CAGCCAGGGT GTAGTTGTCC TGGGGAGGCA 
GGGGCCAACC CCGCCACCAC CACATCTCCG GGGCCTCCGG TTGCCCACAC CCTGTCCAAT 
GCCCTGGTGC CCGGGGGCCC AGCCCCTCCT GCTGAGGCCG ACTTGCACTC GGGGAGCAGC 
AATGACCTGT TCACCACCGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG 
AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTAT ACATGGCGCA GACAGGCGAC 
CCAGCAGCTG AGGAGTGGTC CCCGTGGAGC GTGTGTTCCC TGACGTGTGG GCAGGGTCTG 
CAGGTGCGGA CCCGCTCCTG CGTGTCCTCC CCCTATGGGA CCCTGTGCAG CGGGCCCCTT 
CGGGAGACCC GGCCTTGCAA CAATTCAGCC ACCTGCCCAG TGCACGGCGT GTGGGAGGAG 
TGGGGGTCCT GGAGCCTGTG CTCCCGCAGC TGCGGGCGGG GGTCTCGGAG CCGGATGCGG 
ACCTGCGTGC CCCCCCAGCA CGGCGGCAAG GCCTGCGAGG GTCCCGAGCT GCAGACTAAA 
CTCTGCAGTA TGGCCGCCTG CCCGGTGGAA GGCCAGTGGC TAGAATGGGG TCCCTGGGGC 
CCATGCTCAT CATCTTGTGC CAATGGGACC CAGCAGCGCA GCCGGAAATG CAGTGTGGCG 
GGTCCAGCCT GGGCCACGTG CGCAGGTGCC CTCACGGATA CCCGTGAGTG CAGCAATCTC 
GATTGCCCGG CCACTGACGG CAAGTGGGGG CCGTGGAACG CGTGGAGCCT GTGCTCCAAG 
ACGTGTGACA CGGGCTGGCA ACGCCGCTTC CGCATGTGCC AGGCTTCTGG CACACAGGGC 
TACCCTTGCG AGGGCACAGG AGAGGAGGTG AAACCCTGCA GTGAGAAGAG GTGTCCAGCC 
TTCCATGAGA TGTGCAGGGA TGAGTACGTG ATGTTGATGA CATGGAAGAG GGCGGCAGCT 
GGCGAGATCA TTTACAACAA GTGTCCCCCT AATGCCTCGG GTTCTGCTAG CCGCCGCTGT 
CTCCTCAGTG CCCAGGGCGT AGCATACTGG GGACTGCCCA GCTTTGCTCG TTGCATATCC 
CATGAATACC GCTACCTGTA CCTGTCACTT CGGGAACACC TGGCTAAGGG CCAGCGCATG 
CTGGCAGGTG AGGGCATGTC ACAGGTGGTG CGGAGCCTGC AGGAGCTACT GGCACGGCGC 
ACTTACTACA GCGGGGACCT GCTCTTCTCT GTGGACATCC TAAGGAACGT CACTGACACC 
TTCAAGAGGG CCACCTATGT CCCTTCCGCC GATGACGTGC AGCGTTTCTT CCAGGTGGTG 
AGCTTCATGG TGGATTCAGA AAACAAGGAC AAATGGGATG ATGCTCAGCA GGTGTCACCG 
GGCTCTGTGC ACCTGCTGCG TGTTGTGGAA GATTTCATTC ACCTCGTGGG CGACGCTCTC 
AAGGCCTTCC AGAGCTCTCT CATTGTCACG GACAATCTGG TGATCAGCAT TCAGAGAGAG 
CCTATCTCCG CCGTGTCCAG TGACATCACG TTTCCCATGC GGGGCCGCAG GGGCATGAAG 
GACTGGGTGC GACACTCAGA GGATCGTCTC TTTCTACCCA AGGAGGTGCT CAGCCTGTCC 
TCCCCAGGAA AGCCAGCCAC ACCTGGGGCA GCCACAGCAG GCAGCCCGGG CAGGGGGAGG 
GGCCCAGGAA CGGTGCCCCC TGGCCCAGGC CACGCCCACC AGCGCCTTCT CCCAGCTGAC 
CCCGAAGAGT CCTCCTCCTA CTTTGTGATC GGTGCTGTGC TCTACCGCAC CCTTGGCCTC 
ATCCTGCCGC CCCCCAGGCC TCCACTTGCT GTCACCTCCC GGGTGATGAC AGTGACTGTG 
CGTCCCCCCA CCCAGCCTCC AGCTGAGCCC CTCATTACAG TGGAACTCTC GTACATCATC 
AATGGCACCA CCGATCCCCA CTGTGCCAGC TGGGACTACT CCAGAGCAGA TACCAACTCG 
GGGGACTGGA ACACTGAGAG CTGCCAGACC TTGGAGACCC AGGCGGCTCA CACCCGCTGC 
CAGTGCCAGC ACCTGTCCAC CTTTGCCGTC CTGGCCCAGC CACCCAAGGA CCTGACCCTG 
GAGCTGGCAG GTGCTCCCTC TGTCCCCCTG GTGATCGGCT GTGCAGTGTC CTGCATGGCT 
CTGCTCACCC TGCTGGCCAT CTATGCAGCC TTCTGGAGGT TTATAAAATC AGAACGCTCC 
ATCATCTTGC TGAACTTCTG CCTGTCCATC CTGGCTTCCA ACATTCTGAT CCTGGTGGGC 
CAGTCCCGGG TGCTGAGCAA GGGCGTATGC ACCATGACGG CTGCCTTCCT ACACTTCTTC 
TTTCTGTCCT CCTTTTGCTG GGTGCTTACA GAGGCCTGGC AATCCTATCT GGCTGTCATC 
GGGCGGATGC GCACCCGCCT GGTTCGCAAG CGCTTCCTCT GCCTGGGCTG GGGTCTACCT 
GCCCTGGTGG TGGCTGTGTC TGTTGGCTTT ACTCGCACCA AAGGATATGG TACATCCAGC 
TACTGCTGGC TGTCCCTAGA GGGCGGCCTG CTCTATGCCT TCGTGGGTCC AGCAGCAGTC 
ATTGTCCTGG TGAACATGCT CATCGGGATT ATCGTCTTCA ACAAGCTCAT GGCTCGCGAT 
GGCGTCTCAG ACAAATCTAA GAAGCAGAGG GCTGGGTCGG AGCGGTGCCC CTGGGCCAGC 
CTGCTCCTTC CCTGCTCAGC GTGTGGAGCG GTCCCCAGCC CCCTGCTCAG CTCAGCCTCG 
GCCAGGAATG CCATGGCTTC ACTCTGGAGT TCCTGCGTGG TACTGCCTCT CCTGGCGCTT 
ACCTGGATGT CTGCCGTCCT GGCCATGACA GATCGCCGCT CCGTCCTCTT CCAGGCACTC 
TTTGCCGTTT TCAACTCTGC ACAAGGCTTT GTCATCACCG CTGTGCACTG CTTCCTGCGC 
CGAGAGGTCC AGGATGTGGT AAAGTGTCAG ATGGGTGTGT GTCGGGCTGA TGAGAGTGAA 
GACTCCCCAG ACTCGTGCAA GAACGGGCAG CTGCAGATCC TGTCAGACTT TGAAAAGGAC 
GTGGATCTGG CTTGTCAGAC AGTTCTGTTC AAGGAGGTCA ACACCTGCAA CCCGTCCACC 
ATTACCGGCA CTCTGTCCCG CCTGTCTCTG GATGAGGATG AGGAGCCCAA GTCCTGTCTC 
GTGGGTCCTG AGGGTGGCCT CAGCTTCTCA CCACTGCCTG GGAACATCCT GGTACCCATG 
GCAGCCTCAC CAGGTCTAGG GGAGCCACCA CCACCCCAGG AGACCAACCC TGTGTACATG 
TGTGGGGAGG GTGGCCTGCG GCAGTTGGAC CTTACATGGA TACGGCAGAG TGAACCAGGC 
TCTGAGGGGG ACTACATGGT TCTGCCCCGG CGGACTTTGA GCCTGCAGCC TGGTGGTGGG 
GGTACAGCGG GTGAGGAGGC CCCAAGGGCC CGGCCTGAGG GGACCCCCCG GCGGGCTGCC 
AAAACGGTAG CCCACACTGA AGGCTACCCC AGCTTCCTGT CTGTGGAGCA CTCGGGTCTA 
GGGCTGGGCC CTGCCTATGG GTCTCTCCAG AACCCGTATG GAATGACCTT CCAACCACCA 
CCACCAACAC CCAGCGCCCG CCAAGTACCA GAGCCAGGAG AACGTAGCCG GACCATGCCC 
CGTACAGTGC CTGGTTCCAC CATGAAGCTG GGCTCCCTTG AGCGAAAGAA GCTTCGGTAT 
TCGGACTTGG ACTTTGAGAA GGTGATGCAC ACTCGGAAAC GGCACTCGGA ACTCTACCAC 
GAACTCAACC AGAAGTTCCA CACTTTCGAC CGCTACCGTA GCCAGTCCTC AGCCAAGGAG 
AAACCCAGCC CCCCCGGGGG ACGCCCTGGC TTGTCCCAGC ACAGGAGGCA TCAAAGCTGG 
AGCACCTTCA AATCTATGAC ACTGGGCTCA CTGCCCCCCA AGCCCCGAGA ACGGCTGGCC 
CTGCACCGGA CAGCAGCCTG GGAGCCCACA GAACCGCCAG ACGGCGACTT CCAGACAGAG 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001277644.1
CDS	16..4701
Translation	MTPACPLLLSVILSLRLATAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCTHFAPRLLPLDHYLVNFTCLRPGPEEATARAESEVGRPEEEEEEAAAAASGLELCGGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGANPATTTSPGPPVAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSSSCANGTQQRSRKCSVAGPAWATCAGALTDTRECSNLDCPATDGKWGPWNAWSLCSKTCDTGWQRRFRMCQASGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKRAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDSENKDKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPISAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSSPGKPATPGAATAGSPGRGRGPGTVPPGPGHAHQRLLPADPEESSSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADTNSGDWNTESCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGAPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNILILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGVSDKSKKQRAGSERCPWASLLLPCSACGAVPSPLLSSASARNAMASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGGLSFSPLPGNILVPMAASPGLGEPPPPQETNPVYMCGEGGLRQLDLTWIRQSEPGSEGDYMVLPRRTLSLQPGGGGTAGEEAPRARPEGTPRRAAKTVAHTEGYPSFLSVEHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVPEPGERSRTMPRTVPGSTMKLGSLERKKLRYSDLDFEKVMHTRKRHSELYHELNQKFHTFDRYRSQSSAKEKPSPPGGRPGLSQHRRHQSWSTFKSMTLGSLPPKPRERLALHRTAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	NM_001290715.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001290715.1

ORF Insert Sequence:

ATGACCCCAG CCTGTCCCCT CTTACTGTCT GTGATTCTGT CCCTGCGCCT GGCCACGGCC 
TTCGACCCTG CCCCCAGTGC CTGCTCTGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTC 
TCGCTGCAGG ACCTCTTTCC CACCATCGCC TCGGGCTGCT CCTGGACCCT GGAGAACCCA 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGGC AGGAGCAGGT TTGCACACAC 
TTTGCCCCGC GCCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTTACCTG CCTGCGGCCT 
GGTCCAGAGG AAGCCACAGC CCGGGCTGAG TCGGAGGTGG GACGGCCAGA GGAGGAGGAG 
GAGGAGGCGG CGGCAGCAGC ATCAGGGTTG GAGTTGTGTG GTGGCTCAGG CCCCTTTACC 
TTTCTGCACT TCGACAAGAA CTTTGTGCAG CTGTGCCTGT CGGCTGAGCC CTCTGAGGCC 
CCTCGTCTGC TAGCGCCTGC TGCCCTGGCC TTCCGTTTTG TGGAGGTCTT GCTGATCAAC 
AACAACAACT CCAGCCAGTT CACCTGTGGT GTGCTCTGCC GCTGGAGTGA GGAGTGTGGC 
CGGGCTGCAG GCAGGGCTTG TGGCTTTGCA CAGCCAGGGT GTAGTTGTCC TGGGGAGGCA 
GGGGCCAACC CCGCCACCAC CACATCTCCG GGGCCTCCGG TTGCCCACAC CCTGTCCAAT 
GCCCTGGTGC CCGGGGGCCC AGCCCCTCCT GCTGAGGCCG ACTTGCACTC GGGGAGCAGC 
AATGACCTGT TCACCACCGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG 
AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTAT ACATGGCGCA GACAGGCGAC 
CCAGCAGCTG AGGAGTGGTC CCCGTGGAGC GTGTGTTCCC TGACGTGTGG GCAGGGTCTG 
CAGGTGCGGA CCCGCTCCTG CGTGTCCTCC CCCTATGGGA CCCTGTGCAG CGGGCCCCTT 
CGGGAGACCC GGCCTTGCAA CAATTCAGCC ACCTGCCCAG TGCACGGCGT GTGGGAGGAG 
TGGGGGTCCT GGAGCCTGTG CTCCCGCAGC TGCGGGCGGG GGTCTCGGAG CCGGATGCGG 
ACCTGCGTGC CCCCCCAGCA CGGCGGCAAG GCCTGCGAGG GTCCCGAGCT GCAGACTAAA 
CTCTGCAGTA TGGCCGCCTG CCCGGTGGAA GGCCAGTGGC TAGAATGGGG TCCCTGGGGC 
CCATGCTCAT CATCTTGTGC CAATGGGACC CAGCAGCGCA GCCGGAAATG CAGTGTGGCG 
GGTCCAGCCT GGGCCACGTG CGCAGGTGCC CTCACGGATA CCCGTGAGTG CAGCAATCTC 
GATTGCCCGG CCACTGACGG CAAGTGGGGG CCGTGGAACG CGTGGAGCCT GTGCTCCAAG 
ACGTGTGACA CGGGCTGGCA ACGCCGCTTC CGCATGTGCC AGGCTTCTGG CACACAGGGC 
TACCCTTGCG AGGGCACAGG AGAGGAGGTG AAACCCTGCA GTGAGAAGAG GTGTCCAGCC 
TTCCATGAGA TGTGCAGGGA TGAGTACGTG ATGTTGATGA CATGGAAGAG GGCGGCAGCT 
GGCGAGATCA TTTACAACAA GTGTCCCCCT AATGCCTCGG GTTCTGCTAG CCGCCGCTGT 
CTCCTCAGTG CCCAGGGCGT AGCATACTGG GGACTGCCCA GCTTTGCTCG TTGCATATCC 
CATGAATACC GCTACCTGTA CCTGTCACTT CGGGAACACC TGGCTAAGGG CCAGCGCATG 
CTGGCAGGTG AGGGCATGTC ACAGGTGGTG CGGAGCCTGC AGGAGCTACT GGCACGGCGC 
ACTTACTACA GCGGGGACCT GCTCTTCTCT GTGGACATCC TAAGGAACGT CACTGACACC 
TTCAAGAGGG CCACCTATGT CCCTTCCGCC GATGACGTGC AGCGTTTCTT CCAGGTGGTG 
AGCTTCATGG TGGATTCAGA AAACAAGGAC AAATGGGATG ATGCTCAGCA GGTGTCACCG 
GGCTCTGTGC ACCTGCTGCG TGTTGTGGAA GATTTCATTC ACCTCGTGGG CGACGCTCTC 
AAGGCCTTCC AGAGCTCTCT CATTGTCACG GACAATCTGG TGATCAGCAT TCAGAGAGAG 
CCTATCTCCG CCGTGTCCAG TGACATCACG TTTCCCATGC GGGGCCGCAG GGGCATGAAG 
GACTGGGTGC GACACTCAGA GGATCGTCTC TTTCTACCCA AGGAGGTGCT CAGCCTGTCC 
TCCCCAGGAA AGCCAGCCAC ACCTGGGGCA GCCACAGCAG GCAGCCCGGG CAGGGGGAGG 
GGCCCAGGAA CGGTGCCCCC TGGCCCAGGC CACGCCCACC AGCGCCTTCT CCCAGCTGAC 
CCCGAAGAGT CCTCCTCCTA CTTTGTGATC GGTGCTGTGC TCTACCGCAC CCTTGGCCTC 
ATCCTGCCGC CCCCCAGGCC TCCACTTGCT GTCACCTCCC GGGTGATGAC AGTGACTGTG 
CGTCCCCCCA CCCAGCCTCC AGCTGAGCCC CTCATTACAG TGGAACTCTC GTACATCATC 
AATGGCACCA CCGATCCCCA CTGTGCCAGC TGGGACTACT CCAGAGCAGA TACCAACTCG 
GGGGACTGGA ACACTGAGAG CTGCCAGACC TTGGAGACCC AGGCGGCTCA CACCCGCTGC 
CAGTGCCAGC ACCTGTCCAC CTTTGCCGTC CTGGCCCAGC CACCCAAGGA CCTGACCCTG 
GAGCTGGCAG GTGCTCCCTC TGTCCCCCTG GTGATCGGCT GTGCAGTGTC CTGCATGGCT 
CTGCTCACCC TGCTGGCCAT CTATGCAGCC TTCTGGAGGT TTATAAAATC AGAACGCTCC 
ATCATCTTGC TGAACTTCTG CCTGTCCATC CTGGCTTCCA ACATTCTGAT CCTGGTGGGC 
CAGTCCCGGG TGCTGAGCAA GGGCGTATGC ACCATGACGG CTGCCTTCCT ACACTTCTTC 
TTTCTGTCCT CCTTTTGCTG GGTGCTTACA GAGGCCTGGC AATCCTATCT GGCTGTCATC 
GGGCGGATGC GCACCCGCCT GGTTCGCAAG CGCTTCCTCT GCCTGGGCTG GGGTCTACCT 
GCCCTGGTGG TGGCTGTGTC TGTTGGCTTT ACTCGCACCA AAGGATATGG TACATCCAGC 
TACTGCTGGC TGTCCCTAGA GGGCGGCCTG CTCTATGCCT TCGTGGGTCC AGCAGCAGTC 
ATTGTCCTGG TGAACATGCT CATCGGGATT ATCGTCTTCA ACAAGCTCAT GGCTCGCGAT 
GGCGTCTCAG ACAAATCTAA GAAGCAGAGG GCTGGGTCGG AGCGGTGCCC CTGGGCCAGC 
CTGCTCCTTC CCTGCTCAGC GTGTGGAGCG GTCCCCAGCC CCCTGCTCAG CTCAGCCTCG 
GCCAGGAATG CCATGGCTTC ACTCTGGAGT TCCTGCGTGG TACTGCCTCT CCTGGCGCTT 
ACCTGGATGT CTGCCGTCCT GGCCATGACA GATCGCCGCT CCGTCCTCTT CCAGGCACTC 
TTTGCCGTTT TCAACTCTGC ACAAGGCTTT GTCATCACCG CTGTGCACTG CTTCCTGCGC 
CGAGAGGTCC AGGATGTGGT AAAGTGTCAG ATGGGTGTGT GTCGGGCTGA TGAGAGTGAA 
GACTCCCCAG ACTCGTGCAA GAACGGGCAG CTGCAGATCC TGTCAGACTT TGAAAAGGAC 
GTGGATCTGG CTTGTCAGAC AGTTCTGTTC AAGGAGGTCA ACACCTGCAA CCCGTCCACC 
ATTACCGGCA CTCTGTCCCG CCTGTCTCTG GATGAGGATG AGGAGCCCAA GTCCTGTCTC 
GTGGGTCCTG AGGGTGGCCT CAGCTTCTCA CCACTGCCTG GGAACATCCT GGTACCCATG 
GCAGCCTCAC CAGGTCTAGG GGAGCCACCA CCACCCCAGG AGACCAACCC TGTGTACATG 
TGTGGGGAGG GTGGCCTGCG GCAGTTGGAC CTTACATGGA TACGGCAGAG TGAACCAGGC 
TCTGAGGGGG ACTACATGGT TCTGCCCCGG CGGACTTTGA GCCTGCAGCC TGGTGGTGGG 
GGTACAGCGG GTGAGGAGGC CCCAAGGGCC CGGCCTGAGG GGACCCCCCG GCGGGCTGCC 
AAAACGGTAG CCCACACTGA AGGCTACCCC AGCTTCCTGT CTGTGGAGCA CTCGGGTCTA 
GGGCTGGGCC CTGCCTATGG GTCTCTCCAG AACCCGTATG GAATGACCTT CCAACCACCA 
CCACCAACAC CCAGCGCCCG CCAAGTACCA GAGCCAGGAG AACGTAGCCG GACCATGCCC 
CGTACAGTGC CTGGTTCCAC CATGAAGCTG GGCTCCCTTG AGCGAAAGAA GCTTCGGTAT 
TCGGACTTGG ACTTTGAGAA GGTGATGCAC ACTCGGAAAC GGCACTCGGA ACTCTACCAC 
GAACTCAACC AGAAGTTCCA CACTTTCGAC CGCTACCGTA GCCAGTCCTC AGCCAAGGAG 
AAACCCAGCC CCCCCGGGGG ACGCCCTGGC TTGTCCCAGC ACAGGAGGCA TCAAAGCTGG 
AGCACCTTCA AATCTATGAC ACTGGGCTCA CTGCCCCCCA AGCCCCGAGA ACGGCTGGCC 
CTGCACCGGA CAGCAGCCTG GGAGCCCACA GAACCGCCAG ACGGCGACTT CCAGACAGAG 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adgrb2 ( NM_173071.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_775094.2 Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant 1, mRNA.

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CloneID	OMu14311
Clone ID Related Accession (Same CDS sequence)	NM_173071.3 , NM_173071.4
Accession Version	NM_173071.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4650bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Mus musculus(house mouse)
Product	adhesion G protein-coupled receptor B2 isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ162521.1 and BC056926.1. On Dec 12, 2010 this sequence version replaced NM_173071.2. Transcript Variant: This variant (1) represents the longer transcript and it encodes the longer protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC056926.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCCCAG CCTGTCCCCT CTTACTGTCT GTGATTCTGT CCCTGCGCCT GGCCACGGCC 
TTCGACCCTG CCCCCAGTGC CTGCTCTGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTC 
TCGCTGCAGG ACCTCTTTCC CACCATCGCC TCGGGCTGCT CCTGGACCCT GGAGAACCCA 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGGC AGGAGCAGGT TTGCACACAC 
TTTGCCCCGC GCCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTTACCTG CCTGCGGCCT 
GGTCCAGAGG AAGCCACAGC CCGGGCTGAG TCGGAGGTGG GACGGCCAGA GGAGGAGGAG 
GAGGAGGCGG CGGCAGCAGC ATCAGGGTTG GAGTTGTGTG GTGGCTCAGG CCCCTTTACC 
TTTCTGCACT TCGACAAGAA CTTTGTGCAG CTGTGCCTGT CGGCTGAGCC CTCTGAGGCC 
CCTCGTCTGC TAGCGCCTGC TGCCCTGGCC TTCCGTTTTG TGGAGGTCTT GCTGATCAAC 
AACAACAACT CCAGCCAGTT CACCTGTGGT GTGCTCTGCC GCTGGAGTGA GGAGTGTGGC 
CGGGCTGCAG GCAGGGCTTG TGGCTTTGCA CAGCCAGGGT GTAGTTGTCC TGGGGAGGCA 
GGGGCCAACC CCGCCACCAC CACATCTCCG GGGCCTCCGG TTGCCCACAC CCTGTCCAAT 
GCCCTGGTGC CCGGGGGCCC AGCCCCTCCT GCTGAGGCCG ACTTGCACTC GGGGAGCAGC 
AATGACCTGT TCACCACCGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG 
AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTAT ACATGGCGCA GACAGGCGAC 
CCAGCAGCTG AGGAGTGGTC CCCGTGGAGC GTGTGTTCCC TGACGTGTGG GCAGGGTCTG 
CAGGTGCGGA CCCGCTCCTG CGTGTCCTCC CCCTATGGGA CCCTGTGCAG CGGGCCCCTT 
CGGGAGACCC GGCCTTGCAA CAATTCAGCC ACCTGCCCAG TGCACGGCGT GTGGGAGGAG 
TGGGGGTCCT GGAGCCTGTG CTCCCGCAGC TGCGGGCGGG GGTCTCGGAG CCGGATGCGG 
ACCTGCGTGC CCCCCCAGCA CGGCGGCAAG GCCTGCGAGG GTCCCGAGCT GCAGACTAAA 
CTCTGCAGTA TGGCCGCCTG CCCGGTGGAA GGCCAGTGGC TAGAATGGGG TCCCTGGGGC 
CCATGCTCAT CATCTTGTGC CAATGGGACC CAGCAGCGCA GCCGGAAATG CAGTGTGGCG 
GGTCCAGCCT GGGCCACGTG CGCAGGTGCC CTCACGGATA CCCGTGAGTG CAGCAATCTC 
GATTGCCCGG CCACTGACGG CAAGTGGGGG CCGTGGAACG CGTGGAGCCT GTGCTCCAAG 
ACGTGTGACA CGGGCTGGCA ACGCCGCTTC CGCATGTGCC AGGCTTCTGG CACACAGGGC 
TACCCTTGCG AGGGCACAGG AGAGGAGGTG AAACCCTGCA GTGAGAAGAG GTGTCCAGCC 
TTCCATGAGA TGTGCAGGGA TGAGTACGTG ATGTTGATGA CATGGAAGAG GGCGGCAGCT 
GGCGAGATCA TTTACAACAA GTGTCCCCCT AATGCCTCGG GTTCTGCTAG CCGCCGCTGT 
CTCCTCAGTG CCCAGGGCGT AGCATACTGG GGACTGCCCA GCTTTGCTCG TTGCATATCC 
CATGAATACC GCTACCTGTA CCTGTCACTT CGGGAACACC TGGCTAAGGG CCAGCGCATG 
CTGGCAGGTG AGGGCATGTC ACAGGTGGTG CGGAGCCTGC AGGAGCTACT GGCACGGCGC 
ACTTACTACA GCGGGGACCT GCTCTTCTCT GTGGACATCC TAAGGAACGT CACTGACACC 
TTCAAGAGGG CCACCTATGT CCCTTCCGCC GATGACGTGC AGCGTTTCTT CCAGGTGGTG 
AGCTTCATGG TGGATTCAGA AAACAAGGAC AAATGGGATG ATGCTCAGCA GGTGTCACCG 
GGCTCTGTGC ACCTGCTGCG TGTTGTGGAA GATTTCATTC ACCTCGTGGG CGACGCTCTC 
AAGGCCTTCC AGAGCTCTCT CATTGTCACG GACAATCTGG TGATCAGCAT TCAGAGAGAG 
CCTATCTCCG CCGTGTCCAG TGACATCACG TTTCCCATGC GGGGCCGCAG GGGCATGAAG 
GACTGGGTGC GACACTCAGA GGATCGTCTC TTTCTACCCA AGGAGGTGCT CAGCCTGTCC 
TCCCCAGGAA AGCCAGCCAC ACCTGGGGCA GCCACAGCAG GCAGCCCGGG CAGGGGGAGG 
GGCCCAGGAA CGGTGCCCCC TGGCCCAGGC CACGCCCACC AGCGCCTTCT CCCAGCTGAC 
CCCGAAGAGT CCTCCTCCTA CTTTGTGATC GGTGCTGTGC TCTACCGCAC CCTTGGCCTC 
ATCCTGCCGC CCCCCAGGCC TCCACTTGCT GTCACCTCCC GGGTGATGAC AGTGACTGTG 
CGTCCCCCCA CCCAGCCTCC AGCTGAGCCC CTCATTACAG TGGAACTCTC GTACATCATC 
AATGGCACCA CCGATCCCCA CTGTGCCAGC TGGGACTACT CCAGAGCAGA TACCAACTCG 
GGGGACTGGA ACACTGAGAG CTGCCAGACC TTGGAGACCC AGGCGGCTCA CACCCGCTGC 
CAGTGCCAGC ACCTGTCCAC CTTTGCCGTC CTGGCCCAGC CACCCAAGGA CCTGACCCTG 
GAGCTGGCAG GTGCTCCCTC TGTCCCCCTG GTGATCGGCT GTGCAGTGTC CTGCATGGCT 
CTGCTCACCC TGCTGGCCAT CTATGCAGCC TTCTGGAGGT TTATAAAATC AGAACGCTCC 
ATCATCTTGC TGAACTTCTG CCTGTCCATC CTGGCTTCCA ACATTCTGAT CCTGGTGGGC 
CAGTCCCGGG TGCTGAGCAA GGGCGTATGC ACCATGACGG CTGCCTTCCT ACACTTCTTC 
TTTCTGTCCT CCTTTTGCTG GGTGCTTACA GAGGCCTGGC AATCCTATCT GGCTGTCATC 
GGGCGGATGC GCACCCGCCT GGTTCGCAAG CGCTTCCTCT GCCTGGGCTG GGGTCTACCT 
GCCCTGGTGG TGGCTGTGTC TGTTGGCTTT ACTCGCACCA AAGGATATGG TACATCCAGC 
TACTGCTGGC TGTCCCTAGA GGGCGGCCTG CTCTATGCCT TCGTGGGTCC AGCAGCAGTC 
ATTGTCCTGG TGAACATGCT CATCGGGATT ATCGTCTTCA ACAAGCTCAT GGCTCGCGAT 
GGCGTCTCAG ACAAATCTAA GAAGCAGAGG GCTGGCCGAC TGTCTTGGAA TCTCTGGGGA 
TATGGCTCTC AACTGTGTCT TTTTCCAAAG CTTCCAAGGG CTTCACTCTG GAGTTCCTGC 
GTGGTACTGC CTCTCCTGGC GCTTACCTGG ATGTCTGCCG TCCTGGCCAT GACAGATCGC 
CGCTCCGTCC TCTTCCAGGC ACTCTTTGCC GTTTTCAACT CTGCACAAGG CTTTGTCATC 
ACCGCTGTGC ACTGCTTCCT GCGCCGAGAG GTCCAGGATG TGGTAAAGTG TCAGATGGGT 
GTGTGTCGGG CTGATGAGAG TGAAGACTCC CCAGACTCGT GCAAGAACGG GCAGCTGCAG 
ATCCTGTCAG ACTTTGAAAA GGACGTGGAT CTGGCTTGTC AGACAGTTCT GTTCAAGGAG 
GTCAACACCT GCAACCCGTC CACCATTACC GGCACTCTGT CCCGCCTGTC TCTGGATGAG 
GATGAGGAGC CCAAGTCCTG TCTCGTGGGT CCTGAGGGTG GCCTCAGCTT CTCACCACTG 
CCTGGGAACA TCCTGGTACC CATGGCAGCC TCACCAGGTC TAGGGGAGCC ACCACCACCC 
CAGGAGACCA ACCCTGTGTA CATGTGTGGG GAGGGTGGCC TGCGGCAGTT GGACCTTACA 
TGGATACGGC AGAGTGAACC AGGCTCTGAG GGGGACTACA TGGTTCTGCC CCGGCGGACT 
TTGAGCCTGC AGCCTGGTGG TGGGGGTACA GCGGGTGAGG AGGCCCCAAG GGCCCGGCCT 
GAGGGGACCC CCCGGCGGGC TGCCAAAACG GTAGCCCACA CTGAAGGCTA CCCCAGCTTC 
CTGTCTGTGG AGCACTCGGG TCTAGGGCTG GGCCCTGCCT ATGGGTCTCT CCAGAACCCG 
TATGGAATGA CCTTCCAACC ACCACCACCA ACACCCAGCG CCCGCCAAGT ACCAGAGCCA 
GGAGAACGTA GCCGGACCAT GCCCCGTACA GTGCCTGGTT CCACCATGAA GCTGGGCTCC 
CTTGAGCGAA AGAAGCTTCG GTATTCGGAC TTGGACTTTG AGAAGGTGAT GCACACTCGG 
AAACGGCACT CGGAACTCTA CCACGAACTC AACCAGAAGT TCCACACTTT CGACCGCTAC 
CGTAGCCAGT CCTCAGCCAA GGAGAAACCC AGCCCCCCCG GGGGACGCCC TGGCTTGTCC 
CAGCACAGGA GGCATCAAAG CTGGAGCACC TTCAAATCTA TGACACTGGG CTCACTGCCC 
CCCAAGCCCC GAGAACGGCT GGCCCTGCAC CGGACAGCAG CCTGGGAGCC CACAGAACCG 
CCAGACGGCG ACTTCCAGAC AGAGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775094.2
CDS	246..4895
Translation	MTPACPLLLSVILSLRLATAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCTHFAPRLLPLDHYLVNFTCLRPGPEEATARAESEVGRPEEEEEEAAAAASGLELCGGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGANPATTTSPGPPVAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSSSCANGTQQRSRKCSVAGPAWATCAGALTDTRECSNLDCPATDGKWGPWNAWSLCSKTCDTGWQRRFRMCQASGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKRAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDSENKDKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPISAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSSPGKPATPGAATAGSPGRGRGPGTVPPGPGHAHQRLLPADPEESSSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADTNSGDWNTESCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGAPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNILILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGVSDKSKKQRAGRLSWNLWGYGSQLCLFPKLPRASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGGLSFSPLPGNILVPMAASPGLGEPPPPQETNPVYMCGEGGLRQLDLTWIRQSEPGSEGDYMVLPRRTLSLQPGGGGTAGEEAPRARPEGTPRRAAKTVAHTEGYPSFLSVEHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVPEPGERSRTMPRTVPGSTMKLGSLERKKLRYSDLDFEKVMHTRKRHSELYHELNQKFHTFDRYRSQSSAKEKPSPPGGRPGLSQHRRHQSWSTFKSMTLGSLPPKPRERLALHRTAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	NM_173071.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173071.3

ORF Insert Sequence:

ATGACCCCAG CCTGTCCCCT CTTACTGTCT GTGATTCTGT CCCTGCGCCT GGCCACGGCC 
TTCGACCCTG CCCCCAGTGC CTGCTCTGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTC 
TCGCTGCAGG ACCTCTTTCC CACCATCGCC TCGGGCTGCT CCTGGACCCT GGAGAACCCA 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGGC AGGAGCAGGT TTGCACACAC 
TTTGCCCCGC GCCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTTACCTG CCTGCGGCCT 
GGTCCAGAGG AAGCCACAGC CCGGGCTGAG TCGGAGGTGG GACGGCCAGA GGAGGAGGAG 
GAGGAGGCGG CGGCAGCAGC ATCAGGGTTG GAGTTGTGTG GTGGCTCAGG CCCCTTTACC 
TTTCTGCACT TCGACAAGAA CTTTGTGCAG CTGTGCCTGT CGGCTGAGCC CTCTGAGGCC 
CCTCGTCTGC TAGCGCCTGC TGCCCTGGCC TTCCGTTTTG TGGAGGTCTT GCTGATCAAC 
AACAACAACT CCAGCCAGTT CACCTGTGGT GTGCTCTGCC GCTGGAGTGA GGAGTGTGGC 
CGGGCTGCAG GCAGGGCTTG TGGCTTTGCA CAGCCAGGGT GTAGTTGTCC TGGGGAGGCA 
GGGGCCAACC CCGCCACCAC CACATCTCCG GGGCCTCCGG TTGCCCACAC CCTGTCCAAT 
GCCCTGGTGC CCGGGGGCCC AGCCCCTCCT GCTGAGGCCG ACTTGCACTC GGGGAGCAGC 
AATGACCTGT TCACCACCGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG 
AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTAT ACATGGCGCA GACAGGCGAC 
CCAGCAGCTG AGGAGTGGTC CCCGTGGAGC GTGTGTTCCC TGACGTGTGG GCAGGGTCTG 
CAGGTGCGGA CCCGCTCCTG CGTGTCCTCC CCCTATGGGA CCCTGTGCAG CGGGCCCCTT 
CGGGAGACCC GGCCTTGCAA CAATTCAGCC ACCTGCCCAG TGCACGGCGT GTGGGAGGAG 
TGGGGGTCCT GGAGCCTGTG CTCCCGCAGC TGCGGGCGGG GGTCTCGGAG CCGGATGCGG 
ACCTGCGTGC CCCCCCAGCA CGGCGGCAAG GCCTGCGAGG GTCCCGAGCT GCAGACTAAA 
CTCTGCAGTA TGGCCGCCTG CCCGGTGGAA GGCCAGTGGC TAGAATGGGG TCCCTGGGGC 
CCATGCTCAT CATCTTGTGC CAATGGGACC CAGCAGCGCA GCCGGAAATG CAGTGTGGCG 
GGTCCAGCCT GGGCCACGTG CGCAGGTGCC CTCACGGATA CCCGTGAGTG CAGCAATCTC 
GATTGCCCGG CCACTGACGG CAAGTGGGGG CCGTGGAACG CGTGGAGCCT GTGCTCCAAG 
ACGTGTGACA CGGGCTGGCA ACGCCGCTTC CGCATGTGCC AGGCTTCTGG CACACAGGGC 
TACCCTTGCG AGGGCACAGG AGAGGAGGTG AAACCCTGCA GTGAGAAGAG GTGTCCAGCC 
TTCCATGAGA TGTGCAGGGA TGAGTACGTG ATGTTGATGA CATGGAAGAG GGCGGCAGCT 
GGCGAGATCA TTTACAACAA GTGTCCCCCT AATGCCTCGG GTTCTGCTAG CCGCCGCTGT 
CTCCTCAGTG CCCAGGGCGT AGCATACTGG GGACTGCCCA GCTTTGCTCG TTGCATATCC 
CATGAATACC GCTACCTGTA CCTGTCACTT CGGGAACACC TGGCTAAGGG CCAGCGCATG 
CTGGCAGGTG AGGGCATGTC ACAGGTGGTG CGGAGCCTGC AGGAGCTACT GGCACGGCGC 
ACTTACTACA GCGGGGACCT GCTCTTCTCT GTGGACATCC TAAGGAACGT CACTGACACC 
TTCAAGAGGG CCACCTATGT CCCTTCCGCC GATGACGTGC AGCGTTTCTT CCAGGTGGTG 
AGCTTCATGG TGGATTCAGA AAACAAGGAC AAATGGGATG ATGCTCAGCA GGTGTCACCG 
GGCTCTGTGC ACCTGCTGCG TGTTGTGGAA GATTTCATTC ACCTCGTGGG CGACGCTCTC 
AAGGCCTTCC AGAGCTCTCT CATTGTCACG GACAATCTGG TGATCAGCAT TCAGAGAGAG 
CCTATCTCCG CCGTGTCCAG TGACATCACG TTTCCCATGC GGGGCCGCAG GGGCATGAAG 
GACTGGGTGC GACACTCAGA GGATCGTCTC TTTCTACCCA AGGAGGTGCT CAGCCTGTCC 
TCCCCAGGAA AGCCAGCCAC ACCTGGGGCA GCCACAGCAG GCAGCCCGGG CAGGGGGAGG 
GGCCCAGGAA CGGTGCCCCC TGGCCCAGGC CACGCCCACC AGCGCCTTCT CCCAGCTGAC 
CCCGAAGAGT CCTCCTCCTA CTTTGTGATC GGTGCTGTGC TCTACCGCAC CCTTGGCCTC 
ATCCTGCCGC CCCCCAGGCC TCCACTTGCT GTCACCTCCC GGGTGATGAC AGTGACTGTG 
CGTCCCCCCA CCCAGCCTCC AGCTGAGCCC CTCATTACAG TGGAACTCTC GTACATCATC 
AATGGCACCA CCGATCCCCA CTGTGCCAGC TGGGACTACT CCAGAGCAGA TACCAACTCG 
GGGGACTGGA ACACTGAGAG CTGCCAGACC TTGGAGACCC AGGCGGCTCA CACCCGCTGC 
CAGTGCCAGC ACCTGTCCAC CTTTGCCGTC CTGGCCCAGC CACCCAAGGA CCTGACCCTG 
GAGCTGGCAG GTGCTCCCTC TGTCCCCCTG GTGATCGGCT GTGCAGTGTC CTGCATGGCT 
CTGCTCACCC TGCTGGCCAT CTATGCAGCC TTCTGGAGGT TTATAAAATC AGAACGCTCC 
ATCATCTTGC TGAACTTCTG CCTGTCCATC CTGGCTTCCA ACATTCTGAT CCTGGTGGGC 
CAGTCCCGGG TGCTGAGCAA GGGCGTATGC ACCATGACGG CTGCCTTCCT ACACTTCTTC 
TTTCTGTCCT CCTTTTGCTG GGTGCTTACA GAGGCCTGGC AATCCTATCT GGCTGTCATC 
GGGCGGATGC GCACCCGCCT GGTTCGCAAG CGCTTCCTCT GCCTGGGCTG GGGTCTACCT 
GCCCTGGTGG TGGCTGTGTC TGTTGGCTTT ACTCGCACCA AAGGATATGG TACATCCAGC 
TACTGCTGGC TGTCCCTAGA GGGCGGCCTG CTCTATGCCT TCGTGGGTCC AGCAGCAGTC 
ATTGTCCTGG TGAACATGCT CATCGGGATT ATCGTCTTCA ACAAGCTCAT GGCTCGCGAT 
GGCGTCTCAG ACAAATCTAA GAAGCAGAGG GCTGGCCGAC TGTCTTGGAA TCTCTGGGGA 
TATGGCTCTC AACTGTGTCT TTTTCCAAAG CTTCCAAGGG CTTCACTCTG GAGTTCCTGC 
GTGGTACTGC CTCTCCTGGC GCTTACCTGG ATGTCTGCCG TCCTGGCCAT GACAGATCGC 
CGCTCCGTCC TCTTCCAGGC ACTCTTTGCC GTTTTCAACT CTGCACAAGG CTTTGTCATC 
ACCGCTGTGC ACTGCTTCCT GCGCCGAGAG GTCCAGGATG TGGTAAAGTG TCAGATGGGT 
GTGTGTCGGG CTGATGAGAG TGAAGACTCC CCAGACTCGT GCAAGAACGG GCAGCTGCAG 
ATCCTGTCAG ACTTTGAAAA GGACGTGGAT CTGGCTTGTC AGACAGTTCT GTTCAAGGAG 
GTCAACACCT GCAACCCGTC CACCATTACC GGCACTCTGT CCCGCCTGTC TCTGGATGAG 
GATGAGGAGC CCAAGTCCTG TCTCGTGGGT CCTGAGGGTG GCCTCAGCTT CTCACCACTG 
CCTGGGAACA TCCTGGTACC CATGGCAGCC TCACCAGGTC TAGGGGAGCC ACCACCACCC 
CAGGAGACCA ACCCTGTGTA CATGTGTGGG GAGGGTGGCC TGCGGCAGTT GGACCTTACA 
TGGATACGGC AGAGTGAACC AGGCTCTGAG GGGGACTACA TGGTTCTGCC CCGGCGGACT 
TTGAGCCTGC AGCCTGGTGG TGGGGGTACA GCGGGTGAGG AGGCCCCAAG GGCCCGGCCT 
GAGGGGACCC CCCGGCGGGC TGCCAAAACG GTAGCCCACA CTGAAGGCTA CCCCAGCTTC 
CTGTCTGTGG AGCACTCGGG TCTAGGGCTG GGCCCTGCCT ATGGGTCTCT CCAGAACCCG 
TATGGAATGA CCTTCCAACC ACCACCACCA ACACCCAGCG CCCGCCAAGT ACCAGAGCCA 
GGAGAACGTA GCCGGACCAT GCCCCGTACA GTGCCTGGTT CCACCATGAA GCTGGGCTCC 
CTTGAGCGAA AGAAGCTTCG GTATTCGGAC TTGGACTTTG AGAAGGTGAT GCACACTCGG 
AAACGGCACT CGGAACTCTA CCACGAACTC AACCAGAAGT TCCACACTTT CGACCGCTAC 
CGTAGCCAGT CCTCAGCCAA GGAGAAACCC AGCCCCCCCG GGGGACGCCC TGGCTTGTCC 
CAGCACAGGA GGCATCAAAG CTGGAGCACC TTCAAATCTA TGACACTGGG CTCACTGCCC 
CCCAAGCCCC GAGAACGGCT GGCCCTGCAC CGGACAGCAG CCTGGGAGCC CACAGAACCG 
CCAGACGGCG ACTTCCAGAC AGAGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adgrb2 ( NM_001290714.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001277643.1 Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant 4, mRNA.

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CloneID	OMu16824
Clone ID Related Accession (Same CDS sequence)	NM_001290714.1 , NM_001290714.2
Accession Version	NM_001290714.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Mus musculus(house mouse)
Product	adhesion G protein-coupled receptor B2 isoform 4 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK147485.1, AK144559.1 and BC056926.1. On or before Mar 19, 2014 this sequence version replaced XM_006536978.1, XM_006503056.1. Transcript Variant: This variant (4) lacks one alternate in-frame exon each, in the 5' and 3' coding regions, compared to variant 1. It encodes isoform 4, which is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK147485.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849379 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCCCAG CCTGTCCCCT CTTACTGTCT GTGATTCTGT CCCTGCGCCT GGCCACGGCC 
TTCGACCCTG CCCCCAGTGC CTGCTCTGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTC 
TCGCTGCAGG ACCTCTTTCC CACCATCGCC TCGGGCTGCT CCTGGACCCT GGAGAACCCA 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGGC AGGAGCAGGT TTGCACACAC 
TTTGCCCCGC GCCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTTACCTG CCTGCGGCCT 
GGTCCAGAGG AAGCCACAGC CCGGGCTGAG TCGGAGGTGG GACGGCCAGA GGAGGAGGAG 
GAGGAGGCGG CGGCAGCAGC ATCAGGGTTG GAGTTGTGTG GTGGCTCAGG CCCCTTTACC 
TTTCTGCACT TCGACAAGAA CTTTGTGCAG CTGTGCCTGT CGGCTGAGCC CTCTGAGGCC 
CCTCGTCTGC TAGCGCCTGC TGCCCTGGCC TTCCGTTTTG TGGAGGTCTT GCTGATCAAC 
AACAACAACT CCAGCCAGTT CACCTGTGGT GTGCTCTGCC GCTGGAGTGA GGAGTGTGGC 
CGGGCTGCAG GCAGGGCTTG TGGCTTTGCA CAGCCAGGGT GTAGTTGTCC TGGGGAGGCA 
GGGGCCAACC CCGCCACCAC CACATCTCCG GGGCCTCCGG TTGCCCACAC CCTGTCCAAT 
GCCCTGGTGC CCGGGGGCCC AGCCCCTCCT GCTGAGGCCG ACTTGCACTC GGGGAGCAGC 
AATGACCTGT TCACCACCGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG 
AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTAT ACATGGCGCA GACAGGCGAC 
CCAGCAGCTG AGGAGTGGTC CCCGTGGAGC GTGTGTTCCC TGACGTGTGG GCAGGGTCTG 
CAGGTGCGGA CCCGCTCCTG CGTGTCCTCC CCCTATGGGA CCCTGTGCAG CGGGCCCCTT 
CGGGAGACCC GGCCTTGCAA CAATTCAGCC ACCTGCCCAG TGGAAGGCCA GTGGCTAGAA 
TGGGGTCCCT GGGGCCCATG CTCATCATCT TGTGCCAATG GGACCCAGCA GCGCAGCCGG 
AAATGCAGTG TGGCGGGTCC AGCCTGGGCC ACGTGCGCAG GTGCCCTCAC GGATACCCGT 
GAGTGCAGCA ATCTCGATTG CCCGGCCACT GACGGCAAGT GGGGGCCGTG GAACGCGTGG 
AGCCTGTGCT CCAAGACGTG TGACACGGGC TGGCAACGCC GCTTCCGCAT GTGCCAGGCT 
TCTGGCACAC AGGGCTACCC TTGCGAGGGC ACAGGAGAGG AGGTGAAACC CTGCAGTGAG 
AAGAGGTGTC CAGCCTTCCA TGAGATGTGC AGGGATGAGT ACGTGATGTT GATGACATGG 
AAGAGGGCGG CAGCTGGCGA GATCATTTAC AACAAGTGTC CCCCTAATGC CTCGGGTTCT 
GCTAGCCGCC GCTGTCTCCT CAGTGCCCAG GGCGTAGCAT ACTGGGGACT GCCCAGCTTT 
GCTCGTTGCA TATCCCATGA ATACCGCTAC CTGTACCTGT CACTTCGGGA ACACCTGGCT 
AAGGGCCAGC GCATGCTGGC AGGTGAGGGC ATGTCACAGG TGGTGCGGAG CCTGCAGGAG 
CTACTGGCAC GGCGCACTTA CTACAGCGGG GACCTGCTCT TCTCTGTGGA CATCCTAAGG 
AACGTCACTG ACACCTTCAA GAGGGCCACC TATGTCCCTT CCGCCGATGA CGTGCAGCGT 
TTCTTCCAGG TGGTGAGCTT CATGGTGGAT TCAGAAAACA AGGACAAATG GGATGATGCT 
CAGCAGGTGT CACCGGGCTC TGTGCACCTG CTGCGTGTTG TGGAAGATTT CATTCACCTC 
GTGGGCGACG CTCTCAAGGC CTTCCAGAGC TCTCTCATTG TCACGGACAA TCTGGTGATC 
AGCATTCAGA GAGAGCCTAT CTCCGCCGTG TCCAGTGACA TCACGTTTCC CATGCGGGGC 
CGCAGGGGCA TGAAGGACTG GGTGCGACAC TCAGAGGATC GTCTCTTTCT ACCCAAGGAG 
GTGCTCAGCC TGTCCTCCCC AGGAAAGCCA GCCACACCTG GGGCAGCCAC AGCAGGCAGC 
CCGGGCAGGG GGAGGGGCCC AGGAACGGTG CCCCCTGGCC CAGGCCACGC CCACCAGCGC 
CTTCTCCCAG CTGACCCCGA AGAGTCCTCC TCCTACTTTG TGATCGGTGC TGTGCTCTAC 
CGCACCCTTG GCCTCATCCT GCCGCCCCCC AGGCCTCCAC TTGCTGTCAC CTCCCGGGTG 
ATGACAGTGA CTGTGCGTCC CCCCACCCAG CCTCCAGCTG AGCCCCTCAT TACAGTGGAA 
CTCTCGTACA TCATCAATGG CACCACCGAT CCCCACTGTG CCAGCTGGGA CTACTCCAGA 
GCAGATACCA ACTCGGGGGA CTGGAACACT GAGAGCTGCC AGACCTTGGA GACCCAGGCG 
GCTCACACCC GCTGCCAGTG CCAGCACCTG TCCACCTTTG CCGTCCTGGC CCAGCCACCC 
AAGGACCTGA CCCTGGAGCT GGCAGGTGCT CCCTCTGTCC CCCTGGTGAT CGGCTGTGCA 
GTGTCCTGCA TGGCTCTGCT CACCCTGCTG GCCATCTATG CAGCCTTCTG GAGGTTTATA 
AAATCAGAAC GCTCCATCAT CTTGCTGAAC TTCTGCCTGT CCATCCTGGC TTCCAACATT 
CTGATCCTGG TGGGCCAGTC CCGGGTGCTG AGCAAGGGCG TATGCACCAT GACGGCTGCC 
TTCCTACACT TCTTCTTTCT GTCCTCCTTT TGCTGGGTGC TTACAGAGGC CTGGCAATCC 
TATCTGGCTG TCATCGGGCG GATGCGCACC CGCCTGGTTC GCAAGCGCTT CCTCTGCCTG 
GGCTGGGGTC TACCTGCCCT GGTGGTGGCT GTGTCTGTTG GCTTTACTCG CACCAAAGGA 
TATGGTACAT CCAGCTACTG CTGGCTGTCC CTAGAGGGCG GCCTGCTCTA TGCCTTCGTG 
GGTCCAGCAG CAGTCATTGT CCTGGTGAAC ATGCTCATCG GGATTATCGT CTTCAACAAG 
CTCATGGCTC GCGATGGCGT CTCAGACAAA TCTAAGAAGC AGAGGGCTGG GGCTTCACTC 
TGGAGTTCCT GCGTGGTACT GCCTCTCCTG GCGCTTACCT GGATGTCTGC CGTCCTGGCC 
ATGACAGATC GCCGCTCCGT CCTCTTCCAG GCACTCTTTG CCGTTTTCAA CTCTGCACAA 
GGCTTTGTCA TCACCGCTGT GCACTGCTTC CTGCGCCGAG AGGTCCAGGA TGTGGTAAAG 
TGTCAGATGG GTGTGTGTCG GGCTGATGAG AGTGAAGACT CCCCAGACTC GTGCAAGAAC 
GGGCAGCTGC AGATCCTGTC AGACTTTGAA AAGGACGTGG ATCTGGCTTG TCAGACAGTT 
CTGTTCAAGG AGGTCAACAC CTGCAACCCG TCCACCATTA CCGGCACTCT GTCCCGCCTG 
TCTCTGGATG AGGATGAGGA GCCCAAGTCC TGTCTCGTGG GTCCTGAGGG TGGCCTCAGC 
TTCTCACCAC TGCCTGGGAA CATCCTGGTA CCCATGGCAG CCTCACCAGG TCTAGGGGAG 
CCACCACCAC CCCAGGAGAC CAACCCTGTG TACATGTGTG GGGAGGGTGG CCTGCGGCAG 
TTGGACCTTA CATGGATACG GCAGAGTGAA CCAGGCTCTG AGGGGGACTA CATGGTTCTG 
CCCCGGCGGA CTTTGAGCCT GCAGCCTGGT GGTGGGGGTA CAGCGGGTGA GGAGGCCCCA 
AGGGCCCGGC CTGAGGGGAC CCCCCGGCGG GCTGCCAAAA CGGTAGCCCA CACTGAAGGC 
TACCCCAGCT TCCTGTCTGT GGAGCACTCG GGTCTAGGGC TGGGCCCTGC CTATGGGTCT 
CTCCAGAACC CGTATGGAAT GACCTTCCAA CCACCACCAC CAACACCCAG CGCCCGCCAA 
GTACCAGAGC CAGGAGAACG TAGCCGGACC ATGCCCCGTA CAGTGCCTGG TTCCACCATG 
AAGCTGGGCT CCCTTGAGCG AAAGAAGCTT CGGTATTCGG ACTTGGACTT TGAGAAGGTG 
ATGCACACTC GGAAACGGCA CTCGGAACTC TACCACGAAC TCAACCAGAA GTTCCACACT 
TTCGACCGCT ACCGTAGCCA GTCCTCAGCC AAGGAGAAAC CCAGCCCCCC CGGGGGACGC 
CCTGGCTTGT CCCAGCACAG GAGGCATCAA AGCTGGAGCA CCTTCAAATC TATGACACTG 
GGCTCACTGC CCCCCAAGCC CCGAGAACGG CTGGCCCTGC ACCGGACAGC AGCCTGGGAG 
CCCACAGAAC CGCCAGACGG CGACTTCCAG ACAGAGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001277643.1
CDS	454..4875
Translation	MTPACPLLLSVILSLRLATAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCTHFAPRLLPLDHYLVNFTCLRPGPEEATARAESEVGRPEEEEEEAAAAASGLELCGGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGANPATTTSPGPPVAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVEGQWLEWGPWGPCSSSCANGTQQRSRKCSVAGPAWATCAGALTDTRECSNLDCPATDGKWGPWNAWSLCSKTCDTGWQRRFRMCQASGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKRAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDSENKDKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPISAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSSPGKPATPGAATAGSPGRGRGPGTVPPGPGHAHQRLLPADPEESSSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADTNSGDWNTESCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGAPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNILILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGVSDKSKKQRAGASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGGLSFSPLPGNILVPMAASPGLGEPPPPQETNPVYMCGEGGLRQLDLTWIRQSEPGSEGDYMVLPRRTLSLQPGGGGTAGEEAPRARPEGTPRRAAKTVAHTEGYPSFLSVEHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVPEPGERSRTMPRTVPGSTMKLGSLERKKLRYSDLDFEKVMHTRKRHSELYHELNQKFHTFDRYRSQSSAKEKPSPPGGRPGLSQHRRHQSWSTFKSMTLGSLPPKPRERLALHRTAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	NM_001290714.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001290714.1

ORF Insert Sequence:

ATGACCCCAG CCTGTCCCCT CTTACTGTCT GTGATTCTGT CCCTGCGCCT GGCCACGGCC 
TTCGACCCTG CCCCCAGTGC CTGCTCTGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTC 
TCGCTGCAGG ACCTCTTTCC CACCATCGCC TCGGGCTGCT CCTGGACCCT GGAGAACCCA 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGGC AGGAGCAGGT TTGCACACAC 
TTTGCCCCGC GCCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTTACCTG CCTGCGGCCT 
GGTCCAGAGG AAGCCACAGC CCGGGCTGAG TCGGAGGTGG GACGGCCAGA GGAGGAGGAG 
GAGGAGGCGG CGGCAGCAGC ATCAGGGTTG GAGTTGTGTG GTGGCTCAGG CCCCTTTACC 
TTTCTGCACT TCGACAAGAA CTTTGTGCAG CTGTGCCTGT CGGCTGAGCC CTCTGAGGCC 
CCTCGTCTGC TAGCGCCTGC TGCCCTGGCC TTCCGTTTTG TGGAGGTCTT GCTGATCAAC 
AACAACAACT CCAGCCAGTT CACCTGTGGT GTGCTCTGCC GCTGGAGTGA GGAGTGTGGC 
CGGGCTGCAG GCAGGGCTTG TGGCTTTGCA CAGCCAGGGT GTAGTTGTCC TGGGGAGGCA 
GGGGCCAACC CCGCCACCAC CACATCTCCG GGGCCTCCGG TTGCCCACAC CCTGTCCAAT 
GCCCTGGTGC CCGGGGGCCC AGCCCCTCCT GCTGAGGCCG ACTTGCACTC GGGGAGCAGC 
AATGACCTGT TCACCACCGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG 
AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTAT ACATGGCGCA GACAGGCGAC 
CCAGCAGCTG AGGAGTGGTC CCCGTGGAGC GTGTGTTCCC TGACGTGTGG GCAGGGTCTG 
CAGGTGCGGA CCCGCTCCTG CGTGTCCTCC CCCTATGGGA CCCTGTGCAG CGGGCCCCTT 
CGGGAGACCC GGCCTTGCAA CAATTCAGCC ACCTGCCCAG TGGAAGGCCA GTGGCTAGAA 
TGGGGTCCCT GGGGCCCATG CTCATCATCT TGTGCCAATG GGACCCAGCA GCGCAGCCGG 
AAATGCAGTG TGGCGGGTCC AGCCTGGGCC ACGTGCGCAG GTGCCCTCAC GGATACCCGT 
GAGTGCAGCA ATCTCGATTG CCCGGCCACT GACGGCAAGT GGGGGCCGTG GAACGCGTGG 
AGCCTGTGCT CCAAGACGTG TGACACGGGC TGGCAACGCC GCTTCCGCAT GTGCCAGGCT 
TCTGGCACAC AGGGCTACCC TTGCGAGGGC ACAGGAGAGG AGGTGAAACC CTGCAGTGAG 
AAGAGGTGTC CAGCCTTCCA TGAGATGTGC AGGGATGAGT ACGTGATGTT GATGACATGG 
AAGAGGGCGG CAGCTGGCGA GATCATTTAC AACAAGTGTC CCCCTAATGC CTCGGGTTCT 
GCTAGCCGCC GCTGTCTCCT CAGTGCCCAG GGCGTAGCAT ACTGGGGACT GCCCAGCTTT 
GCTCGTTGCA TATCCCATGA ATACCGCTAC CTGTACCTGT CACTTCGGGA ACACCTGGCT 
AAGGGCCAGC GCATGCTGGC AGGTGAGGGC ATGTCACAGG TGGTGCGGAG CCTGCAGGAG 
CTACTGGCAC GGCGCACTTA CTACAGCGGG GACCTGCTCT TCTCTGTGGA CATCCTAAGG 
AACGTCACTG ACACCTTCAA GAGGGCCACC TATGTCCCTT CCGCCGATGA CGTGCAGCGT 
TTCTTCCAGG TGGTGAGCTT CATGGTGGAT TCAGAAAACA AGGACAAATG GGATGATGCT 
CAGCAGGTGT CACCGGGCTC TGTGCACCTG CTGCGTGTTG TGGAAGATTT CATTCACCTC 
GTGGGCGACG CTCTCAAGGC CTTCCAGAGC TCTCTCATTG TCACGGACAA TCTGGTGATC 
AGCATTCAGA GAGAGCCTAT CTCCGCCGTG TCCAGTGACA TCACGTTTCC CATGCGGGGC 
CGCAGGGGCA TGAAGGACTG GGTGCGACAC TCAGAGGATC GTCTCTTTCT ACCCAAGGAG 
GTGCTCAGCC TGTCCTCCCC AGGAAAGCCA GCCACACCTG GGGCAGCCAC AGCAGGCAGC 
CCGGGCAGGG GGAGGGGCCC AGGAACGGTG CCCCCTGGCC CAGGCCACGC CCACCAGCGC 
CTTCTCCCAG CTGACCCCGA AGAGTCCTCC TCCTACTTTG TGATCGGTGC TGTGCTCTAC 
CGCACCCTTG GCCTCATCCT GCCGCCCCCC AGGCCTCCAC TTGCTGTCAC CTCCCGGGTG 
ATGACAGTGA CTGTGCGTCC CCCCACCCAG CCTCCAGCTG AGCCCCTCAT TACAGTGGAA 
CTCTCGTACA TCATCAATGG CACCACCGAT CCCCACTGTG CCAGCTGGGA CTACTCCAGA 
GCAGATACCA ACTCGGGGGA CTGGAACACT GAGAGCTGCC AGACCTTGGA GACCCAGGCG 
GCTCACACCC GCTGCCAGTG CCAGCACCTG TCCACCTTTG CCGTCCTGGC CCAGCCACCC 
AAGGACCTGA CCCTGGAGCT GGCAGGTGCT CCCTCTGTCC CCCTGGTGAT CGGCTGTGCA 
GTGTCCTGCA TGGCTCTGCT CACCCTGCTG GCCATCTATG CAGCCTTCTG GAGGTTTATA 
AAATCAGAAC GCTCCATCAT CTTGCTGAAC TTCTGCCTGT CCATCCTGGC TTCCAACATT 
CTGATCCTGG TGGGCCAGTC CCGGGTGCTG AGCAAGGGCG TATGCACCAT GACGGCTGCC 
TTCCTACACT TCTTCTTTCT GTCCTCCTTT TGCTGGGTGC TTACAGAGGC CTGGCAATCC 
TATCTGGCTG TCATCGGGCG GATGCGCACC CGCCTGGTTC GCAAGCGCTT CCTCTGCCTG 
GGCTGGGGTC TACCTGCCCT GGTGGTGGCT GTGTCTGTTG GCTTTACTCG CACCAAAGGA 
TATGGTACAT CCAGCTACTG CTGGCTGTCC CTAGAGGGCG GCCTGCTCTA TGCCTTCGTG 
GGTCCAGCAG CAGTCATTGT CCTGGTGAAC ATGCTCATCG GGATTATCGT CTTCAACAAG 
CTCATGGCTC GCGATGGCGT CTCAGACAAA TCTAAGAAGC AGAGGGCTGG GGCTTCACTC 
TGGAGTTCCT GCGTGGTACT GCCTCTCCTG GCGCTTACCT GGATGTCTGC CGTCCTGGCC 
ATGACAGATC GCCGCTCCGT CCTCTTCCAG GCACTCTTTG CCGTTTTCAA CTCTGCACAA 
GGCTTTGTCA TCACCGCTGT GCACTGCTTC CTGCGCCGAG AGGTCCAGGA TGTGGTAAAG 
TGTCAGATGG GTGTGTGTCG GGCTGATGAG AGTGAAGACT CCCCAGACTC GTGCAAGAAC 
GGGCAGCTGC AGATCCTGTC AGACTTTGAA AAGGACGTGG ATCTGGCTTG TCAGACAGTT 
CTGTTCAAGG AGGTCAACAC CTGCAACCCG TCCACCATTA CCGGCACTCT GTCCCGCCTG 
TCTCTGGATG AGGATGAGGA GCCCAAGTCC TGTCTCGTGG GTCCTGAGGG TGGCCTCAGC 
TTCTCACCAC TGCCTGGGAA CATCCTGGTA CCCATGGCAG CCTCACCAGG TCTAGGGGAG 
CCACCACCAC CCCAGGAGAC CAACCCTGTG TACATGTGTG GGGAGGGTGG CCTGCGGCAG 
TTGGACCTTA CATGGATACG GCAGAGTGAA CCAGGCTCTG AGGGGGACTA CATGGTTCTG 
CCCCGGCGGA CTTTGAGCCT GCAGCCTGGT GGTGGGGGTA CAGCGGGTGA GGAGGCCCCA 
AGGGCCCGGC CTGAGGGGAC CCCCCGGCGG GCTGCCAAAA CGGTAGCCCA CACTGAAGGC 
TACCCCAGCT TCCTGTCTGT GGAGCACTCG GGTCTAGGGC TGGGCCCTGC CTATGGGTCT 
CTCCAGAACC CGTATGGAAT GACCTTCCAA CCACCACCAC CAACACCCAG CGCCCGCCAA 
GTACCAGAGC CAGGAGAACG TAGCCGGACC ATGCCCCGTA CAGTGCCTGG TTCCACCATG 
AAGCTGGGCT CCCTTGAGCG AAAGAAGCTT CGGTATTCGG ACTTGGACTT TGAGAAGGTG 
ATGCACACTC GGAAACGGCA CTCGGAACTC TACCACGAAC TCAACCAGAA GTTCCACACT 
TTCGACCGCT ACCGTAGCCA GTCCTCAGCC AAGGAGAAAC CCAGCCCCCC CGGGGGACGC 
CCTGGCTTGT CCCAGCACAG GAGGCATCAA AGCTGGAGCA CCTTCAAATC TATGACACTG 
GGCTCACTGC CCCCCAAGCC CCGAGAACGG CTGGCCCTGC ACCGGACAGC AGCCTGGGAG 
CCCACAGAAC CGCCAGACGG CGACTTCCAG ACAGAGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adgrb2 ( XM_011240506.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011238808.1 Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu73513
Clone ID Related Accession (Same CDS sequence)	XM_011240506.1
Accession Version	XM_011240506.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	adhesion G protein-coupled receptor B2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCAG CCTGTCCCCT CTTACTGTCT GTGATTCTGT CCCTGCGCCT GGCCACGGCC 
TTCGACCCTG CCCCCAGTGC CTGCTCTGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTC 
TCGCTGCAGG ACCTCTTTCC CACCATCGCC TCGGGCTGCT CCTGGACCCT GGAGAACCCA 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGGC AGGAGCAGGT TTGCACACAC 
TTTGCCCCGC GCCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTTACCTG CCTGCGGCCT 
GGTCCAGAGG AAGCCACAGC CCGGGCTGAG TCGGAGGTGG GACGGCCAGA GGAGGAGGAG 
GAGGAGGCGG CGGCAGCAGC ATCAGGGTTG GAGTTGTGTG GTGGCTCAGG CCCCTTTACC 
TTTCTGCACT TCGACAAGAA CTTTGTGCAG CTGTGCCTGT CGGCTGAGCC CTCTGAGGCC 
CCTCGTCTGC TAGCGCCTGC TGCCCTGGCC TTCCGTTTTG TGGAGGTCTT GCTGATCAAC 
AACAACAACT CCAGCCAGTT CACCTGTGGT GTGCTCTGCC GCTGGAGTGA GGAGTGTGGC 
CGGGCTGCAG GCAGGGCTTG TGGCTTTGCA CAGCCAGGGT GTAGTTGTCC TGGGGAGGCA 
GGGGCCAACC CCGCCACCAC CACATCTCCG GGGCCTCCGG TTGCCCACAC CCTGTCCAAT 
GCCCTGGTGC CCGGGGGCCC AGCCCCTCCT GCTGAGGCCG ACTTGCACTC GGGGAGCAGC 
AATGACCTGT TCACCACCGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG 
AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTAT ACATGGCGCA GACAGGCGAC 
CCAGCAGCTG AGGAGTGGTC CCCGTGGAGC GTGTGTTCCC TGACGTGTGG GCAGGGTCTG 
CAGGTGCGGA CCCGCTCCTG CGTGTCCTCC CCCTATGGGA CCCTGTGCAG CGGGCCCCTT 
CGGGAGACCC GGCCTTGCAA CAATTCAGCC ACCTGCCCAG TGCACGGCGT GTGGGAGGAG 
TGGGGGTCCT GGAGCCTGTG CTCCCGCAGC TGCGGGCGGG GGTCTCGGAG CCGGATGCGG 
ACCTGCGTGC CCCCCCAGCA CGGCGGCAAG GCCTGCGAGG GTCCCGAGCT GCAGACTAAA 
CTCTGCAGTA TGGCCGCCTG CCCGGTGGAA GGCCAGTGGC TAGAATGGGG TCCCTGGGGC 
CCATGCTCAT CATCTTGTGC CAATGGGACC CAGCAGCGCA GCCGGAAATG CAGTGTGGCG 
GGTCCAGCCT GGGCCACGTG CGCAGGTGCC CTCACGGATA CCCGTGAGTG CAGCAATCTC 
GATTGCCCGG CCACTGACGG CAAGTGGGGG CCGTGGAACG CGTGGAGCCT GTGCTCCAAG 
ACGTGTGACA CGGGCTGGCA ACGCCGCTTC CGCATGTGCC AGGCTTCTGG CACACAGGGC 
TACCCTTGCG AGGGCACAGG AGAGGAGGTG AAACCCTGCA GTGAGAAGAG GTGTCCAGCC 
TTCCATGAGA TGTGCAGGGA TGAGTACGTG ATGTTGATGA CATGGAAGAG GGCGGCAGCT 
GGCGAGATCA TTTACAACAA GTGTCCCCCT AATGCCTCGG GTTCTGCTAG CCGCCGCTGT 
CTCCTCAGTG CCCAGGGCGT AGCATACTGG GGACTGCCCA GCTTTGCTCG TTGCATATCC 
CATGAATACC GCTACCTGTA CCTGTCACTT CGGGAACACC TGGCTAAGGG CCAGCGCATG 
CTGGCAGGTG AGGGCATGTC ACAGGTGGTG CGGAGCCTGC AGGAGCTACT GGCACGGCGC 
ACTTACTACA GCGGGGACCT GCTCTTCTCT GTGGACATCC TAAGGAACGT CACTGACACC 
TTCAAGAGGG CCACCTATGT CCCTTCCGCC GATGACGTGC AGCGTTTCTT CCAGGTGGTG 
AGCTTCATGG TGGATTCAGA AAACAAGGAC AAATGGGATG ATGCTCAGCA GGTGTCACCG 
GGCTCTGTGC ACCTGCTGCG TGTTGTGGAA GATTTCATTC ACCTCGTGGG CGACGCTCTC 
AAGGCCTTCC AGAGCTCTCT CATTGTCACG GACAATCTGG TGATCAGCAT TCAGAGAGAG 
CCTATCTCCG CCGTGTCCAG TGACATCACG TTTCCCATGC GGGGCCGCAG GGGCATGAAG 
GACTGGGTGC GACACTCAGA GGATCGTCTC TTTCTACCCA AGGAGGTGCT CAGCCTGTCC 
TCCCCAGGAA AGCCAGCCAC ACCTGGGGCA GCCACAGCAG GCAGCCCGGG CAGGGGGAGG 
GGCCCAGGAA CGGTGCCCCC TGGCCCAGGC CACGCCCACC AGCGCCTTCT CCCAGCTGAC 
CCCGAAGAGT CCTCCTCCTA CTTTGTGATC GGTGCTGTGC TCTACCGCAC CCTTGGCCTC 
ATCCTGCCGC CCCCCAGGCC TCCACTTGCT GTCACCTCCC GGGTGATGAC AGTGACTGTG 
CGTCCCCCCA CCCAGCCTCC AGCTGAGCCC CTCATTACAG TGGAACTCTC GTACATCATC 
AATGGCACCA CCGATCCCCA CTGTGCCAGC TGGGACTACT CCAGAGCAGA TACCAACTCG 
GGGGACTGGA ACACTGAGAG CTGCCAGACC TTGGAGACCC AGGCGGCTCA CACCCGCTGC 
CAGTGCCAGC ACCTGTCCAC CTTTGCCGTC CTGGCCCAGC CACCCAAGGA CCTGACCCTG 
GAGCTGGCAG GTGCTCCCTC TGTCCCCCTG GTGATCGGCT GTGCAGTGTC CTGCATGGCT 
CTGCTCACCC TGCTGGCCAT CTATGCAGCC TTCTGGAGGT TTATAAAATC AGAACGCTCC 
ATCATCTTGC TGAACTTCTG CCTGTCCATC CTGGCTTCCA ACATTCTGAT CCTGGTGGGC 
CAGTCCCGGG TGCTGAGCAA GGGCGTATGC ACCATGACGG CTGCCTTCCT ACACTTCTTC 
TTTCTGTCCT CCTTTTGCTG GGTGCTTACA GAGGCCTGGC AATCCTATCT GGCTGTCATC 
GGGCGGATGC GCACCCGCCT GGTTCGCAAG CGCTTCCTCT GCCTGGGCTG GGGTCTACCT 
GCCCTGGTGG TGGCTGTGTC TGTTGGCTTT ACTCGCACCA AAGGATATGG TACATCCAGC 
TACTGCTGGC TGTCCCTAGA GGGCGGCCTG CTCTATGCCT TCGTGGGTCC AGCAGCAGTC 
ATTGTCCTGG TGAACATGCT CATCGGGATT ATCGTCTTCA ACAAGCTCAT GGCTCGCGAT 
GGCGTCTCAG ACAAATCTAA GAAGCAGAGG GCTGGGTCGG AGCGGTGCCC CTGGGCCAGC 
CTGCTCCTTC CCTGCTCAGC GTGTGGAGCG GTCCCCAGCC CCCTGCTCAG CTCAGCCTCG 
GCCAGGAATG CCATCCGACT GTCTTGGAAT CTCTGGGGAT ATGGCTCTCA ACTGTGTCTT 
TTTCCAAAGC TTCCAAGGGC TTCACTCTGG AGTTCCTGCG TGGTACTGCC TCTCCTGGCG 
CTTACCTGGA TGTCTGCCGT CCTGGCCATG ACAGATCGCC GCTCCGTCCT CTTCCAGGCA 
CTCTTTGCCG TTTTCAACTC TGCACAAGGC TTTGTCATCA CCGCTGTGCA CTGCTTCCTG 
CGCCGAGAGG TCCAGGATGT GGTAAAGTGT CAGATGGGTG TGTGTCGGGC TGATGAGAGT 
GAAGACTCCC CAGACTCGTG CAAGAACGGG CAGCTGCAGA TCCTGTCAGA CTTTGAAAAG 
GACGTGGATC TGGCTTGTCA GACAGTTCTG TTCAAGGAGG TCAACACCTG CAACCCGTCC 
ACCATTACCG GCACTCTGTC CCGCCTGTCT CTGGATGAGG ATGAGGAGCC CAAGTCCTGT 
CTCGTGGGTC CTGAGGGTGG CCTCAGCTTC TCACCACTGC CTGGGAACAT CCTGGTACCC 
ATGGCAGCCT CACCAGGTCT AGGGGAGCCA CCACCACCCC AGGAGACCAA CCCTGTGTAC 
ATGTGTGGGG AGGGTGGCCT GCGGCAGTTG GACCTTACAT GGATACGGCA GAGTGAACCA 
GGCTCTGAGG GGGACTACAT GGTTCTGCCC CGGCGGACTT TGAGCCTGCA GCCTGGTGGT 
GGGGGTACAG CGGGTGAGGA GGCCCCAAGG GCCCGGCCTG AGGGGACCCC CCGGCGGGCT 
GCCAAAACGG TAGCCCACAC TGAAGGCTAC CCCAGCTTCC TGTCTGTGGA GCACTCGGGT 
CTAGGGCTGG GCCCTGCCTA TGGGTCTCTC CAGAACCCGT ATGGAATGAC CTTCCAACCA 
CCACCACCAA CACCCAGCGC CCGCCAAGTA CCAGAGCCAG GAGAACGTAG CCGGACCATG 
CCCCGTACAG TGCCTGGTTC CACCATGAAG CTGGGCTCCC TTGAGCGAAA GAAGCTTCGG 
TATTCGGACT TGGACTTTGA GGTGATGCAC ACTCGGAAAC GGCACTCGGA ACTCTACCAC 
GAACTCAACC AGAAGTTCCA CACTTTCGAC CGCTACCGTA GCCAGTCCTC AGCCAAGGAG 
AAACCCAGCC CCCCCGGGGG ACGCCCTGGC TTGTCCCAGC ACAGGAGGCA TCAAAGCTGG 
AGCACCTTCA AATCTATGAC ACTGGGCTCA CTGCCCCCCA AGCCCCGAGA ACGGCTGGCC 
CTGCACCGGA CAGCAGCCTG GGAGCCCACA GAACCGCCAG ACGGCGACTT CCAGACAGAG 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011238808.1
CDS	16..4761
Translation	MTPACPLLLSVILSLRLATAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCTHFAPRLLPLDHYLVNFTCLRPGPEEATARAESEVGRPEEEEEEAAAAASGLELCGGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGANPATTTSPGPPVAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSSSCANGTQQRSRKCSVAGPAWATCAGALTDTRECSNLDCPATDGKWGPWNAWSLCSKTCDTGWQRRFRMCQASGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKRAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDSENKDKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPISAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSSPGKPATPGAATAGSPGRGRGPGTVPPGPGHAHQRLLPADPEESSSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADTNSGDWNTESCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGAPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNILILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGVSDKSKKQRAGSERCPWASLLLPCSACGAVPSPLLSSASARNAIRLSWNLWGYGSQLCLFPKLPRASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGGLSFSPLPGNILVPMAASPGLGEPPPPQETNPVYMCGEGGLRQLDLTWIRQSEPGSEGDYMVLPRRTLSLQPGGGGTAGEEAPRARPEGTPRRAAKTVAHTEGYPSFLSVEHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVPEPGERSRTMPRTVPGSTMKLGSLERKKLRYSDLDFEVMHTRKRHSELYHELNQKFHTFDRYRSQSSAKEKPSPPGGRPGLSQHRRHQSWSTFKSMTLGSLPPKPRERLALHRTAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_011240506.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240506.1

ORF Insert Sequence:

ATGACCCCAG CCTGTCCCCT CTTACTGTCT GTGATTCTGT CCCTGCGCCT GGCCACGGCC 
TTCGACCCTG CCCCCAGTGC CTGCTCTGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTC 
TCGCTGCAGG ACCTCTTTCC CACCATCGCC TCGGGCTGCT CCTGGACCCT GGAGAACCCA 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGGC AGGAGCAGGT TTGCACACAC 
TTTGCCCCGC GCCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTTACCTG CCTGCGGCCT 
GGTCCAGAGG AAGCCACAGC CCGGGCTGAG TCGGAGGTGG GACGGCCAGA GGAGGAGGAG 
GAGGAGGCGG CGGCAGCAGC ATCAGGGTTG GAGTTGTGTG GTGGCTCAGG CCCCTTTACC 
TTTCTGCACT TCGACAAGAA CTTTGTGCAG CTGTGCCTGT CGGCTGAGCC CTCTGAGGCC 
CCTCGTCTGC TAGCGCCTGC TGCCCTGGCC TTCCGTTTTG TGGAGGTCTT GCTGATCAAC 
AACAACAACT CCAGCCAGTT CACCTGTGGT GTGCTCTGCC GCTGGAGTGA GGAGTGTGGC 
CGGGCTGCAG GCAGGGCTTG TGGCTTTGCA CAGCCAGGGT GTAGTTGTCC TGGGGAGGCA 
GGGGCCAACC CCGCCACCAC CACATCTCCG GGGCCTCCGG TTGCCCACAC CCTGTCCAAT 
GCCCTGGTGC CCGGGGGCCC AGCCCCTCCT GCTGAGGCCG ACTTGCACTC GGGGAGCAGC 
AATGACCTGT TCACCACCGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG 
AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTAT ACATGGCGCA GACAGGCGAC 
CCAGCAGCTG AGGAGTGGTC CCCGTGGAGC GTGTGTTCCC TGACGTGTGG GCAGGGTCTG 
CAGGTGCGGA CCCGCTCCTG CGTGTCCTCC CCCTATGGGA CCCTGTGCAG CGGGCCCCTT 
CGGGAGACCC GGCCTTGCAA CAATTCAGCC ACCTGCCCAG TGCACGGCGT GTGGGAGGAG 
TGGGGGTCCT GGAGCCTGTG CTCCCGCAGC TGCGGGCGGG GGTCTCGGAG CCGGATGCGG 
ACCTGCGTGC CCCCCCAGCA CGGCGGCAAG GCCTGCGAGG GTCCCGAGCT GCAGACTAAA 
CTCTGCAGTA TGGCCGCCTG CCCGGTGGAA GGCCAGTGGC TAGAATGGGG TCCCTGGGGC 
CCATGCTCAT CATCTTGTGC CAATGGGACC CAGCAGCGCA GCCGGAAATG CAGTGTGGCG 
GGTCCAGCCT GGGCCACGTG CGCAGGTGCC CTCACGGATA CCCGTGAGTG CAGCAATCTC 
GATTGCCCGG CCACTGACGG CAAGTGGGGG CCGTGGAACG CGTGGAGCCT GTGCTCCAAG 
ACGTGTGACA CGGGCTGGCA ACGCCGCTTC CGCATGTGCC AGGCTTCTGG CACACAGGGC 
TACCCTTGCG AGGGCACAGG AGAGGAGGTG AAACCCTGCA GTGAGAAGAG GTGTCCAGCC 
TTCCATGAGA TGTGCAGGGA TGAGTACGTG ATGTTGATGA CATGGAAGAG GGCGGCAGCT 
GGCGAGATCA TTTACAACAA GTGTCCCCCT AATGCCTCGG GTTCTGCTAG CCGCCGCTGT 
CTCCTCAGTG CCCAGGGCGT AGCATACTGG GGACTGCCCA GCTTTGCTCG TTGCATATCC 
CATGAATACC GCTACCTGTA CCTGTCACTT CGGGAACACC TGGCTAAGGG CCAGCGCATG 
CTGGCAGGTG AGGGCATGTC ACAGGTGGTG CGGAGCCTGC AGGAGCTACT GGCACGGCGC 
ACTTACTACA GCGGGGACCT GCTCTTCTCT GTGGACATCC TAAGGAACGT CACTGACACC 
TTCAAGAGGG CCACCTATGT CCCTTCCGCC GATGACGTGC AGCGTTTCTT CCAGGTGGTG 
AGCTTCATGG TGGATTCAGA AAACAAGGAC AAATGGGATG ATGCTCAGCA GGTGTCACCG 
GGCTCTGTGC ACCTGCTGCG TGTTGTGGAA GATTTCATTC ACCTCGTGGG CGACGCTCTC 
AAGGCCTTCC AGAGCTCTCT CATTGTCACG GACAATCTGG TGATCAGCAT TCAGAGAGAG 
CCTATCTCCG CCGTGTCCAG TGACATCACG TTTCCCATGC GGGGCCGCAG GGGCATGAAG 
GACTGGGTGC GACACTCAGA GGATCGTCTC TTTCTACCCA AGGAGGTGCT CAGCCTGTCC 
TCCCCAGGAA AGCCAGCCAC ACCTGGGGCA GCCACAGCAG GCAGCCCGGG CAGGGGGAGG 
GGCCCAGGAA CGGTGCCCCC TGGCCCAGGC CACGCCCACC AGCGCCTTCT CCCAGCTGAC 
CCCGAAGAGT CCTCCTCCTA CTTTGTGATC GGTGCTGTGC TCTACCGCAC CCTTGGCCTC 
ATCCTGCCGC CCCCCAGGCC TCCACTTGCT GTCACCTCCC GGGTGATGAC AGTGACTGTG 
CGTCCCCCCA CCCAGCCTCC AGCTGAGCCC CTCATTACAG TGGAACTCTC GTACATCATC 
AATGGCACCA CCGATCCCCA CTGTGCCAGC TGGGACTACT CCAGAGCAGA TACCAACTCG 
GGGGACTGGA ACACTGAGAG CTGCCAGACC TTGGAGACCC AGGCGGCTCA CACCCGCTGC 
CAGTGCCAGC ACCTGTCCAC CTTTGCCGTC CTGGCCCAGC CACCCAAGGA CCTGACCCTG 
GAGCTGGCAG GTGCTCCCTC TGTCCCCCTG GTGATCGGCT GTGCAGTGTC CTGCATGGCT 
CTGCTCACCC TGCTGGCCAT CTATGCAGCC TTCTGGAGGT TTATAAAATC AGAACGCTCC 
ATCATCTTGC TGAACTTCTG CCTGTCCATC CTGGCTTCCA ACATTCTGAT CCTGGTGGGC 
CAGTCCCGGG TGCTGAGCAA GGGCGTATGC ACCATGACGG CTGCCTTCCT ACACTTCTTC 
TTTCTGTCCT CCTTTTGCTG GGTGCTTACA GAGGCCTGGC AATCCTATCT GGCTGTCATC 
GGGCGGATGC GCACCCGCCT GGTTCGCAAG CGCTTCCTCT GCCTGGGCTG GGGTCTACCT 
GCCCTGGTGG TGGCTGTGTC TGTTGGCTTT ACTCGCACCA AAGGATATGG TACATCCAGC 
TACTGCTGGC TGTCCCTAGA GGGCGGCCTG CTCTATGCCT TCGTGGGTCC AGCAGCAGTC 
ATTGTCCTGG TGAACATGCT CATCGGGATT ATCGTCTTCA ACAAGCTCAT GGCTCGCGAT 
GGCGTCTCAG ACAAATCTAA GAAGCAGAGG GCTGGGTCGG AGCGGTGCCC CTGGGCCAGC 
CTGCTCCTTC CCTGCTCAGC GTGTGGAGCG GTCCCCAGCC CCCTGCTCAG CTCAGCCTCG 
GCCAGGAATG CCATCCGACT GTCTTGGAAT CTCTGGGGAT ATGGCTCTCA ACTGTGTCTT 
TTTCCAAAGC TTCCAAGGGC TTCACTCTGG AGTTCCTGCG TGGTACTGCC TCTCCTGGCG 
CTTACCTGGA TGTCTGCCGT CCTGGCCATG ACAGATCGCC GCTCCGTCCT CTTCCAGGCA 
CTCTTTGCCG TTTTCAACTC TGCACAAGGC TTTGTCATCA CCGCTGTGCA CTGCTTCCTG 
CGCCGAGAGG TCCAGGATGT GGTAAAGTGT CAGATGGGTG TGTGTCGGGC TGATGAGAGT 
GAAGACTCCC CAGACTCGTG CAAGAACGGG CAGCTGCAGA TCCTGTCAGA CTTTGAAAAG 
GACGTGGATC TGGCTTGTCA GACAGTTCTG TTCAAGGAGG TCAACACCTG CAACCCGTCC 
ACCATTACCG GCACTCTGTC CCGCCTGTCT CTGGATGAGG ATGAGGAGCC CAAGTCCTGT 
CTCGTGGGTC CTGAGGGTGG CCTCAGCTTC TCACCACTGC CTGGGAACAT CCTGGTACCC 
ATGGCAGCCT CACCAGGTCT AGGGGAGCCA CCACCACCCC AGGAGACCAA CCCTGTGTAC 
ATGTGTGGGG AGGGTGGCCT GCGGCAGTTG GACCTTACAT GGATACGGCA GAGTGAACCA 
GGCTCTGAGG GGGACTACAT GGTTCTGCCC CGGCGGACTT TGAGCCTGCA GCCTGGTGGT 
GGGGGTACAG CGGGTGAGGA GGCCCCAAGG GCCCGGCCTG AGGGGACCCC CCGGCGGGCT 
GCCAAAACGG TAGCCCACAC TGAAGGCTAC CCCAGCTTCC TGTCTGTGGA GCACTCGGGT 
CTAGGGCTGG GCCCTGCCTA TGGGTCTCTC CAGAACCCGT ATGGAATGAC CTTCCAACCA 
CCACCACCAA CACCCAGCGC CCGCCAAGTA CCAGAGCCAG GAGAACGTAG CCGGACCATG 
CCCCGTACAG TGCCTGGTTC CACCATGAAG CTGGGCTCCC TTGAGCGAAA GAAGCTTCGG 
TATTCGGACT TGGACTTTGA GGTGATGCAC ACTCGGAAAC GGCACTCGGA ACTCTACCAC 
GAACTCAACC AGAAGTTCCA CACTTTCGAC CGCTACCGTA GCCAGTCCTC AGCCAAGGAG 
AAACCCAGCC CCCCCGGGGG ACGCCCTGGC TTGTCCCAGC ACAGGAGGCA TCAAAGCTGG 
AGCACCTTCA AATCTATGAC ACTGGGCTCA CTGCCCCCCA AGCCCCGAGA ACGGCTGGCC 
CTGCACCGGA CAGCAGCCTG GGAGCCCACA GAACCGCCAG ACGGCGACTT CCAGACAGAG 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adgrb2 ( XM_011240507.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011238809.1 Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant X2, mRNA.

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CloneID	OMu73514
Clone ID Related Accession (Same CDS sequence)	XM_011240507.1 , XM_011240507.2
Accession Version	XM_011240507.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	adhesion G protein-coupled receptor B2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCAG CCTGTCCCCT CTTACTGTCT GTGATTCTGT CCCTGCGCCT GGCCACGGCC 
TTCGACCCTG CCCCCAGTGC CTGCTCTGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTC 
TCGCTGCAGG ACCTCTTTCC CACCATCGCC TCGGGCTGCT CCTGGACCCT GGAGAACCCA 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGGC AGGAGCAGGT TTGCACACAC 
TTTGCCCCGC GCCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTTACCTG CCTGCGGCCT 
GGTCCAGAGG AAGCCACAGC CCGGGCTGAG TCGGAGGTGG GACGGCCAGA GGAGGAGGAG 
GAGGAGGCGG CGGCAGCAGC ATCAGGGTTG GAGTTGTGTG GTGGCTCAGG CCCCTTTACC 
TTTCTGCACT TCGACAAGAA CTTTGTGCAG CTGTGCCTGT CGGCTGAGCC CTCTGAGGCC 
CCTCGTCTGC TAGCGCCTGC TGCCCTGGCC TTCCGTTTTG TGGAGGTCTT GCTGATCAAC 
AACAACAACT CCAGCCAGTT CACCTGTGGT GTGCTCTGCC GCTGGAGTGA GGAGTGTGGC 
CGGGCTGCAG GCAGGGCTTG TGGCTTTGCA CAGCCAGGGT GTAGTTGTCC TGGGGAGGCA 
GGGGCCAACC CCGCCACCAC CACATCTCCG GGGCCTCCGG TTGCCCACAC CCTGTCCAAT 
GCCCTGGTGC CCGGGGGCCC AGCCCCTCCT GCTGAGGCCG ACTTGCACTC GGGGAGCAGC 
AATGACCTGT TCACCACCGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG 
AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTAT ACATGGCGCA GACAGGCGAC 
CCAGCAGCTG AGGAGTGGTC CCCGTGGAGC GTGTGTTCCC TGACGTGTGG GCAGGGTCTG 
CAGGTGCGGA CCCGCTCCTG CGTGTCCTCC CCCTATGGGA CCCTGTGCAG CGGGCCCCTT 
CGGGAGACCC GGCCTTGCAA CAATTCAGCC ACCTGCCCAG TGCACGGCGT GTGGGAGGAG 
TGGGGGTCCT GGAGCCTGTG CTCCCGCAGC TGCGGGCGGG GGTCTCGGAG CCGGATGCGG 
ACCTGCGTGC CCCCCCAGCA CGGCGGCAAG GCCTGCGAGG GTCCCGAGCT GCAGACTAAA 
CTCTGCAGTA TGGCCGCCTG CCCGGTGGAA GGCCAGTGGC TAGAATGGGG TCCCTGGGGC 
CCATGCTCAT CATCTTGTGC CAATGGGACC CAGCAGCGCA GCCGGAAATG CAGTGTGGCG 
GGTCCAGCCT GGGCCACGTG CGCAGGTGCC CTCACGGATA CCCGTGAGTG CAGCAATCTC 
GATTGCCCGG CCACTGACGG CAAGTGGGGG CCGTGGAACG CGTGGAGCCT GTGCTCCAAG 
ACGTGTGACA CGGGCTGGCA ACGCCGCTTC CGCATGTGCC AGGCTTCTGG CACACAGGGC 
TACCCTTGCG AGGGCACAGG AGAGGAGGTG AAACCCTGCA GTGAGAAGAG GTGTCCAGCC 
TTCCATGAGA TGTGCAGGGA TGAGTACGTG ATGTTGATGA CATGGAAGAG GGCGGCAGCT 
GGCGAGATCA TTTACAACAA GTGTCCCCCT AATGCCTCGG GTTCTGCTAG CCGCCGCTGT 
CTCCTCAGTG CCCAGGGCGT AGCATACTGG GGACTGCCCA GCTTTGCTCG TTGCATATCC 
CATGAATACC GCTACCTGTA CCTGTCACTT CGGGAACACC TGGCTAAGGG CCAGCGCATG 
CTGGCAGGTG AGGGCATGTC ACAGGTGGTG CGGAGCCTGC AGGAGCTACT GGCACGGCGC 
ACTTACTACA GCGGGGACCT GCTCTTCTCT GTGGACATCC TAAGGAACGT CACTGACACC 
TTCAAGAGGG CCACCTATGT CCCTTCCGCC GATGACGTGC AGCGTTTCTT CCAGGTGGTG 
AGCTTCATGG TGGATTCAGA AAACAAGGAC AAATGGGATG ATGCTCAGCA GGTGTCACCG 
GGCTCTGTGC ACCTGCTGCG TGTTGTGGAA GATTTCATTC ACCTCGTGGG CGACGCTCTC 
AAGGCCTTCC AGAGCTCTCT CATTGTCACG GACAATCTGG TGATCAGCAT TCAGAGAGAG 
CCTATCTCCG CCGTGTCCAG TGACATCACG TTTCCCATGC GGGGCCGCAG GGGCATGAAG 
GACTGGGTGC GACACTCAGA GGATCGTCTC TTTCTACCCA AGGAGGTGCT CAGCCTGTCC 
TCCCCAGGAA AGCCAGCCAC ACCTGGGGCA GCCACAGCAG GCAGCCCGGG CAGGGGGAGG 
GGCCCAGGAA CGGTGCCCCC TGGCCCAGGC CACGCCCACC AGCGCCTTCT CCCAGCTGAC 
CCCGAAGAGT CCTCCTCCTA CTTTGTGATC GGTGCTGTGC TCTACCGCAC CCTTGGCCTC 
ATCCTGCCGC CCCCCAGGCC TCCACTTGCT GTCACCTCCC GGGTGATGAC AGTGACTGTG 
CGTCCCCCCA CCCAGCCTCC AGCTGAGCCC CTCATTACAG TGGAACTCTC GTACATCATC 
AATGGCACCA CCGATCCCCA CTGTGCCAGC TGGGACTACT CCAGAGCAGA TACCAACTCG 
GGGGACTGGA ACACTGAGAG CTGCCAGACC TTGGAGACCC AGGCGGCTCA CACCCGCTGC 
CAGTGCCAGC ACCTGTCCAC CTTTGCCGTC CTGGCCCAGC CACCCAAGGA CCTGACCCTG 
GAGCTGGCAG GTGCTCCCTC TGTCCCCCTG GTGATCGGCT GTGCAGTGTC CTGCATGGCT 
CTGCTCACCC TGCTGGCCAT CTATGCAGCC TTCTGGAGGT TTATAAAATC AGAACGCTCC 
ATCATCTTGC TGAACTTCTG CCTGTCCATC CTGGCTTCCA ACATTCTGAT CCTGGTGGGC 
CAGTCCCGGG TGCTGAGCAA GGGCGTATGC ACCATGACGG CTGCCTTCCT ACACTTCTTC 
TTTCTGTCCT CCTTTTGCTG GGTGCTTACA GAGGCCTGGC AATCCTATCT GGCTGTCATC 
GGGCGGATGC GCACCCGCCT GGTTCGCAAG CGCTTCCTCT GCCTGGGCTG GGGTCTACCT 
GCCCTGGTGG TGGCTGTGTC TGTTGGCTTT ACTCGCACCA AAGGATATGG TACATCCAGC 
TACTGCTGGC TGTCCCTAGA GGGCGGCCTG CTCTATGCCT TCGTGGGTCC AGCAGCAGTC 
ATTGTCCTGG TGAACATGCT CATCGGGATT ATCGTCTTCA ACAAGCTCAT GGCTCGCGAT 
GGCGTCTCAG ACAAATCTAA GAAGCAGAGG GCTGGGTCGG AGCGGTGCCC CTGGGCCAGC 
CTGCTCCTTC CCTGCTCAGC GTGTGGAGCG GTCCCCAGCC CCCTGCTCAG CTCAGCCTCG 
GCCAGGAATG CCATCCGACT GTCTTGGAAT CTCTGGGGAT ATGGCTCTCA ACTGTGTCTT 
TTTCCAAAGC TTCCAAGGGC TTCACTCTGG AGTTCCTGCG TGGTACTGCC TCTCCTGGCG 
CTTACCTGGA TGTCTGCCGT CCTGGCCATG ACAGATCGCC GCTCCGTCCT CTTCCAGGCA 
CTCTTTGCCG TTTTCAACTC TGCACAAGGC TTTGTCATCA CCGCTGTGCA CTGCTTCCTG 
CGCCGAGAGG TCCAGGATGT GGTAAAGTGT CAGATGGGTG TGTGTCGGGC TGATGAGAGT 
GAAGACTCCC CAGACTCGTG CAAGAACGGG CAGCTGCAGA TCCTGTCAGA CTTTGAAAAG 
GACGTGGATC TGGCTTGTCA GACAGTTCTG TTCAAGGAGG TCAACACCTG CAACCCGTCC 
ACCATTACCG GCACTCTGTC CCGCCTGTCT CTGGATGAGG ATGAGGAGCC CAAGTCCTGT 
CTCGTGGGTC CTGAGGGTGG CCTCAGCTTC TCACCACTGC CTGGGAACAT CCTGGTACCC 
ATGGCAGCCT CACCAGGTCT AGGGGAGCCA CCACCACCCC AGGAGACCAA CCCTGTGTAC 
ATGTGTGGGG AGGGTGGCCT GCGGCAGTTG GACCTTACAT GGATACGGCA GAGTGAACCA 
GGCTCTGAGG GGGACTACAT GGTTCTGCCC CGGCGGACTT TGAGCCTGCA GCCTGGTGGT 
GGGGGTACAG CGGGTGAGGA GGCCCCAAGG GCCCGGCCTG AGGGGACCCC CCGGCGGGCT 
GCCAAAACGG TAGCCCACAC TGAAGGCTAC CCCAGCTTCC TGTCTGTGGA GCACTCGGGT 
CTAGGGCTGG GCCCTGCCTA TGGGTCTCTC CAGAACCCGT ATGGAATGAC CTTCCAACCA 
CCACCACCAA CACCCAGCGC CCGCCAAGTA CCAGAGCCAG GAGAACGTAG CCGGACCATG 
CCCCGTACAG TGCCTGGTTC CACCATGAAG CTGGGCTCCC TTGAGCGAAA GAAGCTTCGG 
TATTCGGACT TGGACTTTGA GAAGGTGATG CACACTCGGA AACGGCACTC GGAACTCTAC 
CACGAACTCA ACCAGAAGTT CCACACTTTC GACCGCTACC GTAGCCAGTC CTCAGCCAAG 
GAGAAACCCA GCCCCCCCGG GGGACGCCCT GGCTTGTCCC AGCACAGGAG GCATCAAAGC 
TGGAGCACCT TCAAATCTAT GACACTGGGC TCACTGCCCC CCAAGCCCCG AGAACGGCTG 
GCCCTGCACC GGACAGCAGC CTGGGAGCCC ACAGAACCGC CAGACGGCGA CTTCCAGACA 
GAGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011238809.1
CDS	241..4989
Translation	MTPACPLLLSVILSLRLATAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCTHFAPRLLPLDHYLVNFTCLRPGPEEATARAESEVGRPEEEEEEAAAAASGLELCGGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGANPATTTSPGPPVAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSSSCANGTQQRSRKCSVAGPAWATCAGALTDTRECSNLDCPATDGKWGPWNAWSLCSKTCDTGWQRRFRMCQASGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKRAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDSENKDKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPISAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSSPGKPATPGAATAGSPGRGRGPGTVPPGPGHAHQRLLPADPEESSSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADTNSGDWNTESCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGAPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNILILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGVSDKSKKQRAGSERCPWASLLLPCSACGAVPSPLLSSASARNAIRLSWNLWGYGSQLCLFPKLPRASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGGLSFSPLPGNILVPMAASPGLGEPPPPQETNPVYMCGEGGLRQLDLTWIRQSEPGSEGDYMVLPRRTLSLQPGGGGTAGEEAPRARPEGTPRRAAKTVAHTEGYPSFLSVEHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVPEPGERSRTMPRTVPGSTMKLGSLERKKLRYSDLDFEKVMHTRKRHSELYHELNQKFHTFDRYRSQSSAKEKPSPPGGRPGLSQHRRHQSWSTFKSMTLGSLPPKPRERLALHRTAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_011240507.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240507.1

ORF Insert Sequence:

ATGACCCCAG CCTGTCCCCT CTTACTGTCT GTGATTCTGT CCCTGCGCCT GGCCACGGCC 
TTCGACCCTG CCCCCAGTGC CTGCTCTGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTC 
TCGCTGCAGG ACCTCTTTCC CACCATCGCC TCGGGCTGCT CCTGGACCCT GGAGAACCCA 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGGC AGGAGCAGGT TTGCACACAC 
TTTGCCCCGC GCCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTTACCTG CCTGCGGCCT 
GGTCCAGAGG AAGCCACAGC CCGGGCTGAG TCGGAGGTGG GACGGCCAGA GGAGGAGGAG 
GAGGAGGCGG CGGCAGCAGC ATCAGGGTTG GAGTTGTGTG GTGGCTCAGG CCCCTTTACC 
TTTCTGCACT TCGACAAGAA CTTTGTGCAG CTGTGCCTGT CGGCTGAGCC CTCTGAGGCC 
CCTCGTCTGC TAGCGCCTGC TGCCCTGGCC TTCCGTTTTG TGGAGGTCTT GCTGATCAAC 
AACAACAACT CCAGCCAGTT CACCTGTGGT GTGCTCTGCC GCTGGAGTGA GGAGTGTGGC 
CGGGCTGCAG GCAGGGCTTG TGGCTTTGCA CAGCCAGGGT GTAGTTGTCC TGGGGAGGCA 
GGGGCCAACC CCGCCACCAC CACATCTCCG GGGCCTCCGG TTGCCCACAC CCTGTCCAAT 
GCCCTGGTGC CCGGGGGCCC AGCCCCTCCT GCTGAGGCCG ACTTGCACTC GGGGAGCAGC 
AATGACCTGT TCACCACCGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG 
AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTAT ACATGGCGCA GACAGGCGAC 
CCAGCAGCTG AGGAGTGGTC CCCGTGGAGC GTGTGTTCCC TGACGTGTGG GCAGGGTCTG 
CAGGTGCGGA CCCGCTCCTG CGTGTCCTCC CCCTATGGGA CCCTGTGCAG CGGGCCCCTT 
CGGGAGACCC GGCCTTGCAA CAATTCAGCC ACCTGCCCAG TGCACGGCGT GTGGGAGGAG 
TGGGGGTCCT GGAGCCTGTG CTCCCGCAGC TGCGGGCGGG GGTCTCGGAG CCGGATGCGG 
ACCTGCGTGC CCCCCCAGCA CGGCGGCAAG GCCTGCGAGG GTCCCGAGCT GCAGACTAAA 
CTCTGCAGTA TGGCCGCCTG CCCGGTGGAA GGCCAGTGGC TAGAATGGGG TCCCTGGGGC 
CCATGCTCAT CATCTTGTGC CAATGGGACC CAGCAGCGCA GCCGGAAATG CAGTGTGGCG 
GGTCCAGCCT GGGCCACGTG CGCAGGTGCC CTCACGGATA CCCGTGAGTG CAGCAATCTC 
GATTGCCCGG CCACTGACGG CAAGTGGGGG CCGTGGAACG CGTGGAGCCT GTGCTCCAAG 
ACGTGTGACA CGGGCTGGCA ACGCCGCTTC CGCATGTGCC AGGCTTCTGG CACACAGGGC 
TACCCTTGCG AGGGCACAGG AGAGGAGGTG AAACCCTGCA GTGAGAAGAG GTGTCCAGCC 
TTCCATGAGA TGTGCAGGGA TGAGTACGTG ATGTTGATGA CATGGAAGAG GGCGGCAGCT 
GGCGAGATCA TTTACAACAA GTGTCCCCCT AATGCCTCGG GTTCTGCTAG CCGCCGCTGT 
CTCCTCAGTG CCCAGGGCGT AGCATACTGG GGACTGCCCA GCTTTGCTCG TTGCATATCC 
CATGAATACC GCTACCTGTA CCTGTCACTT CGGGAACACC TGGCTAAGGG CCAGCGCATG 
CTGGCAGGTG AGGGCATGTC ACAGGTGGTG CGGAGCCTGC AGGAGCTACT GGCACGGCGC 
ACTTACTACA GCGGGGACCT GCTCTTCTCT GTGGACATCC TAAGGAACGT CACTGACACC 
TTCAAGAGGG CCACCTATGT CCCTTCCGCC GATGACGTGC AGCGTTTCTT CCAGGTGGTG 
AGCTTCATGG TGGATTCAGA AAACAAGGAC AAATGGGATG ATGCTCAGCA GGTGTCACCG 
GGCTCTGTGC ACCTGCTGCG TGTTGTGGAA GATTTCATTC ACCTCGTGGG CGACGCTCTC 
AAGGCCTTCC AGAGCTCTCT CATTGTCACG GACAATCTGG TGATCAGCAT TCAGAGAGAG 
CCTATCTCCG CCGTGTCCAG TGACATCACG TTTCCCATGC GGGGCCGCAG GGGCATGAAG 
GACTGGGTGC GACACTCAGA GGATCGTCTC TTTCTACCCA AGGAGGTGCT CAGCCTGTCC 
TCCCCAGGAA AGCCAGCCAC ACCTGGGGCA GCCACAGCAG GCAGCCCGGG CAGGGGGAGG 
GGCCCAGGAA CGGTGCCCCC TGGCCCAGGC CACGCCCACC AGCGCCTTCT CCCAGCTGAC 
CCCGAAGAGT CCTCCTCCTA CTTTGTGATC GGTGCTGTGC TCTACCGCAC CCTTGGCCTC 
ATCCTGCCGC CCCCCAGGCC TCCACTTGCT GTCACCTCCC GGGTGATGAC AGTGACTGTG 
CGTCCCCCCA CCCAGCCTCC AGCTGAGCCC CTCATTACAG TGGAACTCTC GTACATCATC 
AATGGCACCA CCGATCCCCA CTGTGCCAGC TGGGACTACT CCAGAGCAGA TACCAACTCG 
GGGGACTGGA ACACTGAGAG CTGCCAGACC TTGGAGACCC AGGCGGCTCA CACCCGCTGC 
CAGTGCCAGC ACCTGTCCAC CTTTGCCGTC CTGGCCCAGC CACCCAAGGA CCTGACCCTG 
GAGCTGGCAG GTGCTCCCTC TGTCCCCCTG GTGATCGGCT GTGCAGTGTC CTGCATGGCT 
CTGCTCACCC TGCTGGCCAT CTATGCAGCC TTCTGGAGGT TTATAAAATC AGAACGCTCC 
ATCATCTTGC TGAACTTCTG CCTGTCCATC CTGGCTTCCA ACATTCTGAT CCTGGTGGGC 
CAGTCCCGGG TGCTGAGCAA GGGCGTATGC ACCATGACGG CTGCCTTCCT ACACTTCTTC 
TTTCTGTCCT CCTTTTGCTG GGTGCTTACA GAGGCCTGGC AATCCTATCT GGCTGTCATC 
GGGCGGATGC GCACCCGCCT GGTTCGCAAG CGCTTCCTCT GCCTGGGCTG GGGTCTACCT 
GCCCTGGTGG TGGCTGTGTC TGTTGGCTTT ACTCGCACCA AAGGATATGG TACATCCAGC 
TACTGCTGGC TGTCCCTAGA GGGCGGCCTG CTCTATGCCT TCGTGGGTCC AGCAGCAGTC 
ATTGTCCTGG TGAACATGCT CATCGGGATT ATCGTCTTCA ACAAGCTCAT GGCTCGCGAT 
GGCGTCTCAG ACAAATCTAA GAAGCAGAGG GCTGGGTCGG AGCGGTGCCC CTGGGCCAGC 
CTGCTCCTTC CCTGCTCAGC GTGTGGAGCG GTCCCCAGCC CCCTGCTCAG CTCAGCCTCG 
GCCAGGAATG CCATCCGACT GTCTTGGAAT CTCTGGGGAT ATGGCTCTCA ACTGTGTCTT 
TTTCCAAAGC TTCCAAGGGC TTCACTCTGG AGTTCCTGCG TGGTACTGCC TCTCCTGGCG 
CTTACCTGGA TGTCTGCCGT CCTGGCCATG ACAGATCGCC GCTCCGTCCT CTTCCAGGCA 
CTCTTTGCCG TTTTCAACTC TGCACAAGGC TTTGTCATCA CCGCTGTGCA CTGCTTCCTG 
CGCCGAGAGG TCCAGGATGT GGTAAAGTGT CAGATGGGTG TGTGTCGGGC TGATGAGAGT 
GAAGACTCCC CAGACTCGTG CAAGAACGGG CAGCTGCAGA TCCTGTCAGA CTTTGAAAAG 
GACGTGGATC TGGCTTGTCA GACAGTTCTG TTCAAGGAGG TCAACACCTG CAACCCGTCC 
ACCATTACCG GCACTCTGTC CCGCCTGTCT CTGGATGAGG ATGAGGAGCC CAAGTCCTGT 
CTCGTGGGTC CTGAGGGTGG CCTCAGCTTC TCACCACTGC CTGGGAACAT CCTGGTACCC 
ATGGCAGCCT CACCAGGTCT AGGGGAGCCA CCACCACCCC AGGAGACCAA CCCTGTGTAC 
ATGTGTGGGG AGGGTGGCCT GCGGCAGTTG GACCTTACAT GGATACGGCA GAGTGAACCA 
GGCTCTGAGG GGGACTACAT GGTTCTGCCC CGGCGGACTT TGAGCCTGCA GCCTGGTGGT 
GGGGGTACAG CGGGTGAGGA GGCCCCAAGG GCCCGGCCTG AGGGGACCCC CCGGCGGGCT 
GCCAAAACGG TAGCCCACAC TGAAGGCTAC CCCAGCTTCC TGTCTGTGGA GCACTCGGGT 
CTAGGGCTGG GCCCTGCCTA TGGGTCTCTC CAGAACCCGT ATGGAATGAC CTTCCAACCA 
CCACCACCAA CACCCAGCGC CCGCCAAGTA CCAGAGCCAG GAGAACGTAG CCGGACCATG 
CCCCGTACAG TGCCTGGTTC CACCATGAAG CTGGGCTCCC TTGAGCGAAA GAAGCTTCGG 
TATTCGGACT TGGACTTTGA GAAGGTGATG CACACTCGGA AACGGCACTC GGAACTCTAC 
CACGAACTCA ACCAGAAGTT CCACACTTTC GACCGCTACC GTAGCCAGTC CTCAGCCAAG 
GAGAAACCCA GCCCCCCCGG GGGACGCCCT GGCTTGTCCC AGCACAGGAG GCATCAAAGC 
TGGAGCACCT TCAAATCTAT GACACTGGGC TCACTGCCCC CCAAGCCCCG AGAACGGCTG 
GCCCTGCACC GGACAGCAGC CTGGGAGCCC ACAGAACCGC CAGACGGCGA CTTCCAGACA 
GAGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adgrb2 ( XM_011240508.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011238810.1 Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant X3, mRNA.

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CloneID	OMu73515
Clone ID Related Accession (Same CDS sequence)	XM_011240508.1
Accession Version	XM_011240508.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4683bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	adhesion G protein-coupled receptor B2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCAG CCTGTCCCCT CTTACTGTCT GTGATTCTGT CCCTGCGCCT GGCCACGGCC 
TTCGACCCTG CCCCCAGTGC CTGCTCTGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTC 
TCGCTGCAGG ACCTCTTTCC CACCATCGCC TCGGGCTGCT CCTGGACCCT GGAGAACCCA 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGGC AGGAGCAGGT TTGCACACAC 
TTTGCCCCGC GCCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTTACCTG CCTGCGGCCT 
GGTCCAGAGG AAGCCACAGC CCGGGCTGAG TCGGAGGTGG GACGGCCAGA GGAGGAGGAG 
GAGGAGGCGG CGGCAGCAGC ATCAGGGTTG GAGTTGTGTG GTGGCTCAGG CCCCTTTACC 
TTTCTGCACT TCGACAAGAA CTTTGTGCAG CTGTGCCTGT CGGCTGAGCC CTCTGAGGCC 
CCTCGTCTGC TAGCGCCTGC TGCCCTGGCC TTCCGTTTTG TGGAGGTCTT GCTGATCAAC 
AACAACAACT CCAGCCAGTT CACCTGTGGT GTGCTCTGCC GCTGGAGTGA GGAGTGTGGC 
CGGGCTGCAG GCAGGGCTTG TGGCTTTGCA CAGCCAGGGT GTAGTTGTCC TGGGGAGGCA 
GGGGCCAACC CCGCCACCAC CACATCTCCG GGGCCTCCGG TTGCCCACAC CCTGTCCAAT 
GCCCTGGTGC CCGGGGGCCC AGCCCCTCCT GCTGAGGCCG ACTTGCACTC GGGGAGCAGC 
AATGACCTGT TCACCACCGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG 
AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTAT ACATGGCGCA GACAGGCGAC 
CCAGCAGCTG AGGAGTGGTC CCCGTGGAGC GTGTGTTCCC TGACGTGTGG GCAGGGTCTG 
CAGGTGCGGA CCCGCTCCTG CGTGTCCTCC CCCTATGGGA CCCTGTGCAG CGGGCCCCTT 
CGGGAGACCC GGCCTTGCAA CAATTCAGCC ACCTGCCCAG TGCACGGCGT GTGGGAGGAG 
TGGGGGTCCT GGAGCCTGTG CTCCCGCAGC TGCGGGCGGG GGTCTCGGAG CCGGATGCGG 
ACCTGCGTGC CCCCCCAGCA CGGCGGCAAG GCCTGCGAGG GTCCCGAGCT GCAGACTAAA 
CTCTGCAGTA TGGCCGCCTG CCCGGTGGAA GGCCAGTGGC TAGAATGGGG TCCCTGGGGC 
CCATGCTCAT CATCTTGTGC CAATGGGACC CAGCAGCGCA GCCGGAAATG CAGTGTGGCG 
GGTCCAGCCT GGGCCACGTG CGCAGGTGCC CTCACGGATA CCCGTGAGTG CAGCAATCTC 
GATTGCCCGG CCACTGACGG CAAGTGGGGG CCGTGGAACG CGTGGAGCCT GTGCTCCAAG 
ACGTGTGACA CGGGCTGGCA ACGCCGCTTC CGCATGTGCC AGGCTTCTGG CACACAGGGC 
TACCCTTGCG AGGGCACAGG AGAGGAGGTG AAACCCTGCA GTGAGAAGAG GTGTCCAGCC 
TTCCATGAGA TGTGCAGGGA TGAGTACGTG ATGTTGATGA CATGGAAGAG GGCGGCAGCT 
GGCGAGATCA TTTACAACAA GTGTCCCCCT AATGCCTCGG GTTCTGCTAG CCGCCGCTGT 
CTCCTCAGTG CCCAGGGCGT AGCATACTGG GGACTGCCCA GCTTTGCTCG TTGCATATCC 
CATGAATACC GCTACCTGTA CCTGTCACTT CGGGAACACC TGGCTAAGGG CCAGCGCATG 
CTGGCAGGTG AGGGCATGTC ACAGGTGGTG CGGAGCCTGC AGGAGCTACT GGCACGGCGC 
ACTTACTACA GCGGGGACCT GCTCTTCTCT GTGGACATCC TAAGGAACGT CACTGACACC 
TTCAAGAGGG CCACCTATGT CCCTTCCGCC GATGACGTGC AGCGTTTCTT CCAGGTGGTG 
AGCTTCATGG TGGATTCAGA AAACAAGGAC AAATGGGATG ATGCTCAGCA GGTGTCACCG 
GGCTCTGTGC ACCTGCTGCG TGTTGTGGAA GATTTCATTC ACCTCGTGGG CGACGCTCTC 
AAGGCCTTCC AGAGCTCTCT CATTGTCACG GACAATCTGG TGATCAGCAT TCAGAGAGAG 
CCTATCTCCG CCGTGTCCAG TGACATCACG TTTCCCATGC GGGGCCGCAG GGGCATGAAG 
GACTGGGTGC GACACTCAGA GGATCGTCTC TTTCTACCCA AGGAGGTGCT CAGCCTGTCC 
TCCCCAGGAA AGCCAGCCAC ACCTGGGGCA GCCACAGCAG GCAGCCCGGG CAGGGGGAGG 
GGCCCAGGAA CGGTGCCCCC TGGCCCAGGC CACGCCCACC AGCGCCTTCT CCCAGCTGAC 
CCCGAAGAGT CCTCCTCCTA CTTTGTGATC GGTGCTGTGC TCTACCGCAC CCTTGGCCTC 
ATCCTGCCGC CCCCCAGGCC TCCACTTGCT GTCACCTCCC GGGTGATGAC AGTGACTGTG 
CGTCCCCCCA CCCAGCCTCC AGCTGAGCCC CTCATTACAG TGGAACTCTC GTACATCATC 
AATGGCACCA CCGATCCCCA CTGTGCCAGC TGGGACTACT CCAGAGCAGA TACCAACTCG 
GGGGACTGGA ACACTGAGAG CTGCCAGACC TTGGAGACCC AGGCGGCTCA CACCCGCTGC 
CAGTGCCAGC ACCTGTCCAC CTTTGCCGTC CTGGCCCAGC CACCCAAGGA CCTGACCCTG 
GAGCTGGCAG GTGCTCCCTC TGTCCCCCTG GTGATCGGCT GTGCAGTGTC CTGCATGGCT 
CTGCTCACCC TGCTGGCCAT CTATGCAGCC TTCTGGAGGT TTATAAAATC AGAACGCTCC 
ATCATCTTGC TGAACTTCTG CCTGTCCATC CTGGCTTCCA ACATTCTGAT CCTGGTGGGC 
CAGTCCCGGG TGCTGAGCAA GGGCGTATGC ACCATGACGG CTGCCTTCCT ACACTTCTTC 
TTTCTGTCCT CCTTTTGCTG GGTGCTTACA GAGGCCTGGC AATCCTATCT GGCTGTCATC 
GGGCGGATGC GCACCCGCCT GGTTCGCAAG CGCTTCCTCT GCCTGGGCTG GGGTCTACCT 
GCCCTGGTGG TGGCTGTGTC TGTTGGCTTT ACTCGCACCA AAGGATATGG TACATCCAGC 
TACTGCTGGC TGTCCCTAGA GGGCGGCCTG CTCTATGCCT TCGTGGGTCC AGCAGCAGTC 
ATTGTCCTGG TGAACATGCT CATCGGGATT ATCGTCTTCA ACAAGCTCAT GGCTCGCGAT 
GGCGTCTCAG ACAAATCTAA GAAGCAGAGG GCTGGGTCGG AGCGGTGCCC CTGGGCCAGC 
CTGCTCCTTC CCTGCTCAGC GTGTGGAGCG GTCCCCAGCC CCCTGCTCAG CTCAGCCTCG 
GCCAGGAATG CCATGGCTTC ACTCTGGAGT TCCTGCGTGG TACTGCCTCT CCTGGCGCTT 
ACCTGGATGT CTGCCGTCCT GGCCATGACA GATCGCCGCT CCGTCCTCTT CCAGGCACTC 
TTTGCCGTTT TCAACTCTGC ACAAGGCTTT GTCATCACCG CTGTGCACTG CTTCCTGCGC 
CGAGAGGTCC AGGATGTGGT AAAGTGTCAG ATGGGTGTGT GTCGGGCTGA TGAGAGTGAA 
GACTCCCCAG ACTCGTGCAA GAACGGGCAG CTGCAGATCC TGTCAGACTT TGAAAAGGAC 
GTGGATCTGG CTTGTCAGAC AGTTCTGTTC AAGGAGGTCA ACACCTGCAA CCCGTCCACC 
ATTACCGGCA CTCTGTCCCG CCTGTCTCTG GATGAGGATG AGGAGCCCAA GTCCTGTCTC 
GTGGGTCCTG AGGGTGGCCT CAGCTTCTCA CCACTGCCTG GGAACATCCT GGTACCCATG 
GCAGCCTCAC CAGGTCTAGG GGAGCCACCA CCACCCCAGG AGACCAACCC TGTGTACATG 
TGTGGGGAGG GTGGCCTGCG GCAGTTGGAC CTTACATGGA TACGGCAGAG TGAACCAGGC 
TCTGAGGGGG ACTACATGGT TCTGCCCCGG CGGACTTTGA GCCTGCAGCC TGGTGGTGGG 
GGTACAGCGG GTGAGGAGGC CCCAAGGGCC CGGCCTGAGG GGACCCCCCG GCGGGCTGCC 
AAAACGGTAG CCCACACTGA AGGCTACCCC AGCTTCCTGT CTGTGGAGCA CTCGGGTCTA 
GGGCTGGGCC CTGCCTATGG GTCTCTCCAG AACCCGTATG GAATGACCTT CCAACCACCA 
CCACCAACAC CCAGCGCCCG CCAAGTACCA GAGCCAGGAG AACGTAGCCG GACCATGCCC 
CGTACAGTGC CTGGTTCCAC CATGAAGCTG GGCTCCCTTG AGCGAAAGAA GCTTCGGTAT 
TCGGACTTGG ACTTTGAGGT GATGCACACT CGGAAACGGC ACTCGGAACT CTACCACGAA 
CTCAACCAGA AGTTCCACAC TTTCGACCGC TACCGTAGCC AGTCCTCAGC CAAGGAGAAA 
CCCAGCCCCC CCGGGGGACG CCCTGGCTTG TCCCAGCACA GGAGGCATCA AAGCTGGAGC 
ACCTTCAAAT CTATGACACT GGGCTCACTG CCCCCCAAGC CCCGAGAACG GCTGGCCCTG 
CACCGGACAG CAGCCTGGGA GCCCACAGAA CCGCCAGACG GCGACTTCCA GACAGAGGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011238810.1
CDS	16..4698
Translation	MTPACPLLLSVILSLRLATAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCTHFAPRLLPLDHYLVNFTCLRPGPEEATARAESEVGRPEEEEEEAAAAASGLELCGGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGANPATTTSPGPPVAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSSSCANGTQQRSRKCSVAGPAWATCAGALTDTRECSNLDCPATDGKWGPWNAWSLCSKTCDTGWQRRFRMCQASGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKRAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDSENKDKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPISAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSSPGKPATPGAATAGSPGRGRGPGTVPPGPGHAHQRLLPADPEESSSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADTNSGDWNTESCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGAPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNILILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGVSDKSKKQRAGSERCPWASLLLPCSACGAVPSPLLSSASARNAMASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGGLSFSPLPGNILVPMAASPGLGEPPPPQETNPVYMCGEGGLRQLDLTWIRQSEPGSEGDYMVLPRRTLSLQPGGGGTAGEEAPRARPEGTPRRAAKTVAHTEGYPSFLSVEHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVPEPGERSRTMPRTVPGSTMKLGSLERKKLRYSDLDFEVMHTRKRHSELYHELNQKFHTFDRYRSQSSAKEKPSPPGGRPGLSQHRRHQSWSTFKSMTLGSLPPKPRERLALHRTAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_011240508.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240508.1

ORF Insert Sequence:

ATGACCCCAG CCTGTCCCCT CTTACTGTCT GTGATTCTGT CCCTGCGCCT GGCCACGGCC 
TTCGACCCTG CCCCCAGTGC CTGCTCTGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTC 
TCGCTGCAGG ACCTCTTTCC CACCATCGCC TCGGGCTGCT CCTGGACCCT GGAGAACCCA 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGGC AGGAGCAGGT TTGCACACAC 
TTTGCCCCGC GCCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTTACCTG CCTGCGGCCT 
GGTCCAGAGG AAGCCACAGC CCGGGCTGAG TCGGAGGTGG GACGGCCAGA GGAGGAGGAG 
GAGGAGGCGG CGGCAGCAGC ATCAGGGTTG GAGTTGTGTG GTGGCTCAGG CCCCTTTACC 
TTTCTGCACT TCGACAAGAA CTTTGTGCAG CTGTGCCTGT CGGCTGAGCC CTCTGAGGCC 
CCTCGTCTGC TAGCGCCTGC TGCCCTGGCC TTCCGTTTTG TGGAGGTCTT GCTGATCAAC 
AACAACAACT CCAGCCAGTT CACCTGTGGT GTGCTCTGCC GCTGGAGTGA GGAGTGTGGC 
CGGGCTGCAG GCAGGGCTTG TGGCTTTGCA CAGCCAGGGT GTAGTTGTCC TGGGGAGGCA 
GGGGCCAACC CCGCCACCAC CACATCTCCG GGGCCTCCGG TTGCCCACAC CCTGTCCAAT 
GCCCTGGTGC CCGGGGGCCC AGCCCCTCCT GCTGAGGCCG ACTTGCACTC GGGGAGCAGC 
AATGACCTGT TCACCACCGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG 
AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTAT ACATGGCGCA GACAGGCGAC 
CCAGCAGCTG AGGAGTGGTC CCCGTGGAGC GTGTGTTCCC TGACGTGTGG GCAGGGTCTG 
CAGGTGCGGA CCCGCTCCTG CGTGTCCTCC CCCTATGGGA CCCTGTGCAG CGGGCCCCTT 
CGGGAGACCC GGCCTTGCAA CAATTCAGCC ACCTGCCCAG TGCACGGCGT GTGGGAGGAG 
TGGGGGTCCT GGAGCCTGTG CTCCCGCAGC TGCGGGCGGG GGTCTCGGAG CCGGATGCGG 
ACCTGCGTGC CCCCCCAGCA CGGCGGCAAG GCCTGCGAGG GTCCCGAGCT GCAGACTAAA 
CTCTGCAGTA TGGCCGCCTG CCCGGTGGAA GGCCAGTGGC TAGAATGGGG TCCCTGGGGC 
CCATGCTCAT CATCTTGTGC CAATGGGACC CAGCAGCGCA GCCGGAAATG CAGTGTGGCG 
GGTCCAGCCT GGGCCACGTG CGCAGGTGCC CTCACGGATA CCCGTGAGTG CAGCAATCTC 
GATTGCCCGG CCACTGACGG CAAGTGGGGG CCGTGGAACG CGTGGAGCCT GTGCTCCAAG 
ACGTGTGACA CGGGCTGGCA ACGCCGCTTC CGCATGTGCC AGGCTTCTGG CACACAGGGC 
TACCCTTGCG AGGGCACAGG AGAGGAGGTG AAACCCTGCA GTGAGAAGAG GTGTCCAGCC 
TTCCATGAGA TGTGCAGGGA TGAGTACGTG ATGTTGATGA CATGGAAGAG GGCGGCAGCT 
GGCGAGATCA TTTACAACAA GTGTCCCCCT AATGCCTCGG GTTCTGCTAG CCGCCGCTGT 
CTCCTCAGTG CCCAGGGCGT AGCATACTGG GGACTGCCCA GCTTTGCTCG TTGCATATCC 
CATGAATACC GCTACCTGTA CCTGTCACTT CGGGAACACC TGGCTAAGGG CCAGCGCATG 
CTGGCAGGTG AGGGCATGTC ACAGGTGGTG CGGAGCCTGC AGGAGCTACT GGCACGGCGC 
ACTTACTACA GCGGGGACCT GCTCTTCTCT GTGGACATCC TAAGGAACGT CACTGACACC 
TTCAAGAGGG CCACCTATGT CCCTTCCGCC GATGACGTGC AGCGTTTCTT CCAGGTGGTG 
AGCTTCATGG TGGATTCAGA AAACAAGGAC AAATGGGATG ATGCTCAGCA GGTGTCACCG 
GGCTCTGTGC ACCTGCTGCG TGTTGTGGAA GATTTCATTC ACCTCGTGGG CGACGCTCTC 
AAGGCCTTCC AGAGCTCTCT CATTGTCACG GACAATCTGG TGATCAGCAT TCAGAGAGAG 
CCTATCTCCG CCGTGTCCAG TGACATCACG TTTCCCATGC GGGGCCGCAG GGGCATGAAG 
GACTGGGTGC GACACTCAGA GGATCGTCTC TTTCTACCCA AGGAGGTGCT CAGCCTGTCC 
TCCCCAGGAA AGCCAGCCAC ACCTGGGGCA GCCACAGCAG GCAGCCCGGG CAGGGGGAGG 
GGCCCAGGAA CGGTGCCCCC TGGCCCAGGC CACGCCCACC AGCGCCTTCT CCCAGCTGAC 
CCCGAAGAGT CCTCCTCCTA CTTTGTGATC GGTGCTGTGC TCTACCGCAC CCTTGGCCTC 
ATCCTGCCGC CCCCCAGGCC TCCACTTGCT GTCACCTCCC GGGTGATGAC AGTGACTGTG 
CGTCCCCCCA CCCAGCCTCC AGCTGAGCCC CTCATTACAG TGGAACTCTC GTACATCATC 
AATGGCACCA CCGATCCCCA CTGTGCCAGC TGGGACTACT CCAGAGCAGA TACCAACTCG 
GGGGACTGGA ACACTGAGAG CTGCCAGACC TTGGAGACCC AGGCGGCTCA CACCCGCTGC 
CAGTGCCAGC ACCTGTCCAC CTTTGCCGTC CTGGCCCAGC CACCCAAGGA CCTGACCCTG 
GAGCTGGCAG GTGCTCCCTC TGTCCCCCTG GTGATCGGCT GTGCAGTGTC CTGCATGGCT 
CTGCTCACCC TGCTGGCCAT CTATGCAGCC TTCTGGAGGT TTATAAAATC AGAACGCTCC 
ATCATCTTGC TGAACTTCTG CCTGTCCATC CTGGCTTCCA ACATTCTGAT CCTGGTGGGC 
CAGTCCCGGG TGCTGAGCAA GGGCGTATGC ACCATGACGG CTGCCTTCCT ACACTTCTTC 
TTTCTGTCCT CCTTTTGCTG GGTGCTTACA GAGGCCTGGC AATCCTATCT GGCTGTCATC 
GGGCGGATGC GCACCCGCCT GGTTCGCAAG CGCTTCCTCT GCCTGGGCTG GGGTCTACCT 
GCCCTGGTGG TGGCTGTGTC TGTTGGCTTT ACTCGCACCA AAGGATATGG TACATCCAGC 
TACTGCTGGC TGTCCCTAGA GGGCGGCCTG CTCTATGCCT TCGTGGGTCC AGCAGCAGTC 
ATTGTCCTGG TGAACATGCT CATCGGGATT ATCGTCTTCA ACAAGCTCAT GGCTCGCGAT 
GGCGTCTCAG ACAAATCTAA GAAGCAGAGG GCTGGGTCGG AGCGGTGCCC CTGGGCCAGC 
CTGCTCCTTC CCTGCTCAGC GTGTGGAGCG GTCCCCAGCC CCCTGCTCAG CTCAGCCTCG 
GCCAGGAATG CCATGGCTTC ACTCTGGAGT TCCTGCGTGG TACTGCCTCT CCTGGCGCTT 
ACCTGGATGT CTGCCGTCCT GGCCATGACA GATCGCCGCT CCGTCCTCTT CCAGGCACTC 
TTTGCCGTTT TCAACTCTGC ACAAGGCTTT GTCATCACCG CTGTGCACTG CTTCCTGCGC 
CGAGAGGTCC AGGATGTGGT AAAGTGTCAG ATGGGTGTGT GTCGGGCTGA TGAGAGTGAA 
GACTCCCCAG ACTCGTGCAA GAACGGGCAG CTGCAGATCC TGTCAGACTT TGAAAAGGAC 
GTGGATCTGG CTTGTCAGAC AGTTCTGTTC AAGGAGGTCA ACACCTGCAA CCCGTCCACC 
ATTACCGGCA CTCTGTCCCG CCTGTCTCTG GATGAGGATG AGGAGCCCAA GTCCTGTCTC 
GTGGGTCCTG AGGGTGGCCT CAGCTTCTCA CCACTGCCTG GGAACATCCT GGTACCCATG 
GCAGCCTCAC CAGGTCTAGG GGAGCCACCA CCACCCCAGG AGACCAACCC TGTGTACATG 
TGTGGGGAGG GTGGCCTGCG GCAGTTGGAC CTTACATGGA TACGGCAGAG TGAACCAGGC 
TCTGAGGGGG ACTACATGGT TCTGCCCCGG CGGACTTTGA GCCTGCAGCC TGGTGGTGGG 
GGTACAGCGG GTGAGGAGGC CCCAAGGGCC CGGCCTGAGG GGACCCCCCG GCGGGCTGCC 
AAAACGGTAG CCCACACTGA AGGCTACCCC AGCTTCCTGT CTGTGGAGCA CTCGGGTCTA 
GGGCTGGGCC CTGCCTATGG GTCTCTCCAG AACCCGTATG GAATGACCTT CCAACCACCA 
CCACCAACAC CCAGCGCCCG CCAAGTACCA GAGCCAGGAG AACGTAGCCG GACCATGCCC 
CGTACAGTGC CTGGTTCCAC CATGAAGCTG GGCTCCCTTG AGCGAAAGAA GCTTCGGTAT 
TCGGACTTGG ACTTTGAGGT GATGCACACT CGGAAACGGC ACTCGGAACT CTACCACGAA 
CTCAACCAGA AGTTCCACAC TTTCGACCGC TACCGTAGCC AGTCCTCAGC CAAGGAGAAA 
CCCAGCCCCC CCGGGGGACG CCCTGGCTTG TCCCAGCACA GGAGGCATCA AAGCTGGAGC 
ACCTTCAAAT CTATGACACT GGGCTCACTG CCCCCCAAGC CCCGAGAACG GCTGGCCCTG 
CACCGGACAG CAGCCTGGGA GCCCACAGAA CCGCCAGACG GCGACTTCCA GACAGAGGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adgrb2 ( XM_011240509.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011238811.1 Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant X4, mRNA.

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CloneID	OMu73516
Clone ID Related Accession (Same CDS sequence)	NM_001377056.1 , XM_011240509.1
Accession Version	XM_011240509.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4587bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	adhesion G protein-coupled receptor B2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCAG CCTGTCCCCT CTTACTGTCT GTGATTCTGT CCCTGCGCCT GGCCACGGCC 
TTCGACCCTG CCCCCAGTGC CTGCTCTGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTC 
TCGCTGCAGG ACCTCTTTCC CACCATCGCC TCGGGCTGCT CCTGGACCCT GGAGAACCCA 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGGC AGGAGCAGGT TTGCACACAC 
TTTGCCCCGC GCCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTTACCTG CCTGCGGCCT 
GGTCCAGAGG AAGCCACAGC CCGGGCTGAG TCGGAGGTGG GACGGCCAGA GGAGGAGGAG 
GAGGAGGCGG CGGCAGCAGC ATCAGGGTTG GAGTTGTGTG GTGGCTCAGG CCCCTTTACC 
TTTCTGCACT TCGACAAGAA CTTTGTGCAG CTGTGCCTGT CGGCTGAGCC CTCTGAGGCC 
CCTCGTCTGC TAGCGCCTGC TGCCCTGGCC TTCCGTTTTG TGGAGGTCTT GCTGATCAAC 
AACAACAACT CCAGCCAGTT CACCTGTGGT GTGCTCTGCC GCTGGAGTGA GGAGTGTGGC 
CGGGCTGCAG GCAGGGCTTG TGGCTTTGCA CAGCCAGGGT GTAGTTGTCC TGGGGAGGCA 
GGGGCCAACC CCGCCACCAC CACATCTCCG GGGCCTCCGG TTGCCCACAC CCTGTCCAAT 
GCCCTGGTGC CCGGGGGCCC AGCCCCTCCT GCTGAGGCCG ACTTGCACTC GGGGAGCAGC 
AATGACCTGT TCACCACCGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG 
AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTAT ACATGGCGCA GACAGGCGAC 
CCAGCAGCTG AGGAGTGGTC CCCGTGGAGC GTGTGTTCCC TGACGTGTGG GCAGGGTCTG 
CAGGTGCGGA CCCGCTCCTG CGTGTCCTCC CCCTATGGGA CCCTGTGCAG CGGGCCCCTT 
CGGGAGACCC GGCCTTGCAA CAATTCAGCC ACCTGCCCAG TGCACGGCGT GTGGGAGGAG 
TGGGGGTCCT GGAGCCTGTG CTCCCGCAGC TGCGGGCGGG GGTCTCGGAG CCGGATGCGG 
ACCTGCGTGC CCCCCCAGCA CGGCGGCAAG GCCTGCGAGG GTCCCGAGCT GCAGACTAAA 
CTCTGCAGTA TGGCCGCCTG CCCGGTGGAA GGCCAGTGGC TAGAATGGGG TCCCTGGGGC 
CCATGCTCAT CATCTTGTGC CAATGGGACC CAGCAGCGCA GCCGGAAATG CAGTGTGGCG 
GGTCCAGCCT GGGCCACGTG CGCAGGTGCC CTCACGGATA CCCGTGAGTG CAGCAATCTC 
GATTGCCCGG CCACTGACGG CAAGTGGGGG CCGTGGAACG CGTGGAGCCT GTGCTCCAAG 
ACGTGTGACA CGGGCTGGCA ACGCCGCTTC CGCATGTGCC AGGCTTCTGG CACACAGGGC 
TACCCTTGCG AGGGCACAGG AGAGGAGGTG AAACCCTGCA GTGAGAAGAG GTGTCCAGCC 
TTCCATGAGA TGTGCAGGGA TGAGTACGTG ATGTTGATGA CATGGAAGAG GGCGGCAGCT 
GGCGAGATCA TTTACAACAA GTGTCCCCCT AATGCCTCGG GTTCTGCTAG CCGCCGCTGT 
CTCCTCAGTG CCCAGGGCGT AGCATACTGG GGACTGCCCA GCTTTGCTCG TTGCATATCC 
CATGAATACC GCTACCTGTA CCTGTCACTT CGGGAACACC TGGCTAAGGG CCAGCGCATG 
CTGGCAGGTG AGGGCATGTC ACAGGTGGTG CGGAGCCTGC AGGAGCTACT GGCACGGCGC 
ACTTACTACA GCGGGGACCT GCTCTTCTCT GTGGACATCC TAAGGAACGT CACTGACACC 
TTCAAGAGGG CCACCTATGT CCCTTCCGCC GATGACGTGC AGCGTTTCTT CCAGGTGGTG 
AGCTTCATGG TGGATTCAGA AAACAAGGAC AAATGGGATG ATGCTCAGCA GGTGTCACCG 
GGCTCTGTGC ACCTGCTGCG TGTTGTGGAA GATTTCATTC ACCTCGTGGG CGACGCTCTC 
AAGGCCTTCC AGAGCTCTCT CATTGTCACG GACAATCTGG TGATCAGCAT TCAGAGAGAG 
CCTATCTCCG CCGTGTCCAG TGACATCACG TTTCCCATGC GGGGCCGCAG GGGCATGAAG 
GACTGGGTGC GACACTCAGA GGATCGTCTC TTTCTACCCA AGGAGGTGCT CAGCCTGTCC 
TCCCCAGGAA AGCCAGCCAC ACCTGGGGCA GCCACAGCAG GCAGCCCGGG CAGGGGGAGG 
GGCCCAGGAA CGGTGCCCCC TGGCCCAGGC CACGCCCACC AGCGCCTTCT CCCAGCTGAC 
CCCGAAGAGT CCTCCTCCTA CTTTGTGATC GGTGCTGTGC TCTACCGCAC CCTTGGCCTC 
ATCCTGCCGC CCCCCAGGCC TCCACTTGCT GTCACCTCCC GGGTGATGAC AGTGACTGTG 
CGTCCCCCCA CCCAGCCTCC AGCTGAGCCC CTCATTACAG TGGAACTCTC GTACATCATC 
AATGGCACCA CCGATCCCCA CTGTGCCAGC TGGGACTACT CCAGAGCAGA TACCAACTCG 
GGGGACTGGA ACACTGAGAG CTGCCAGACC TTGGAGACCC AGGCGGCTCA CACCCGCTGC 
CAGTGCCAGC ACCTGTCCAC CTTTGCCGTC CTGGCCCAGC CACCCAAGGA CCTGACCCTG 
GAGCTGGCAG GTGCTCCCTC TGTCCCCCTG GTGATCGGCT GTGCAGTGTC CTGCATGGCT 
CTGCTCACCC TGCTGGCCAT CTATGCAGCC TTCTGGAGGT TTATAAAATC AGAACGCTCC 
ATCATCTTGC TGAACTTCTG CCTGTCCATC CTGGCTTCCA ACATTCTGAT CCTGGTGGGC 
CAGTCCCGGG TGCTGAGCAA GGGCGTATGC ACCATGACGG CTGCCTTCCT ACACTTCTTC 
TTTCTGTCCT CCTTTTGCTG GGTGCTTACA GAGGCCTGGC AATCCTATCT GGCTGTCATC 
GGGCGGATGC GCACCCGCCT GGTTCGCAAG CGCTTCCTCT GCCTGGGCTG GGGTCTACCT 
GCCCTGGTGG TGGCTGTGTC TGTTGGCTTT ACTCGCACCA AAGGATATGG TACATCCAGC 
TACTGCTGGC TGTCCCTAGA GGGCGGCCTG CTCTATGCCT TCGTGGGTCC AGCAGCAGTC 
ATTGTCCTGG TGAACATGCT CATCGGGATT ATCGTCTTCA ACAAGCTCAT GGCTCGCGAT 
GGCGTCTCAG ACAAATCTAA GAAGCAGAGG GCTGGGGCTT CACTCTGGAG TTCCTGCGTG 
GTACTGCCTC TCCTGGCGCT TACCTGGATG TCTGCCGTCC TGGCCATGAC AGATCGCCGC 
TCCGTCCTCT TCCAGGCACT CTTTGCCGTT TTCAACTCTG CACAAGGCTT TGTCATCACC 
GCTGTGCACT GCTTCCTGCG CCGAGAGGTC CAGGATGTGG TAAAGTGTCA GATGGGTGTG 
TGTCGGGCTG ATGAGAGTGA AGACTCCCCA GACTCGTGCA AGAACGGGCA GCTGCAGATC 
CTGTCAGACT TTGAAAAGGA CGTGGATCTG GCTTGTCAGA CAGTTCTGTT CAAGGAGGTC 
AACACCTGCA ACCCGTCCAC CATTACCGGC ACTCTGTCCC GCCTGTCTCT GGATGAGGAT 
GAGGAGCCCA AGTCCTGTCT CGTGGGTCCT GAGGGTGGCC TCAGCTTCTC ACCACTGCCT 
GGGAACATCC TGGTACCCAT GGCAGCCTCA CCAGGTCTAG GGGAGCCACC ACCACCCCAG 
GAGACCAACC CTGTGTACAT GTGTGGGGAG GGTGGCCTGC GGCAGTTGGA CCTTACATGG 
ATACGGCAGA GTGAACCAGG CTCTGAGGGG GACTACATGG TTCTGCCCCG GCGGACTTTG 
AGCCTGCAGC CTGGTGGTGG GGGTACAGCG GGTGAGGAGG CCCCAAGGGC CCGGCCTGAG 
GGGACCCCCC GGCGGGCTGC CAAAACGGTA GCCCACACTG AAGGCTACCC CAGCTTCCTG 
TCTGTGGAGC ACTCGGGTCT AGGGCTGGGC CCTGCCTATG GGTCTCTCCA GAACCCGTAT 
GGAATGACCT TCCAACCACC ACCACCAACA CCCAGCGCCC GCCAAGTACC AGAGCCAGGA 
GAACGTAGCC GGACCATGCC CCGTACAGTG CCTGGTTCCA CCATGAAGCT GGGCTCCCTT 
GAGCGAAAGA AGCTTCGGTA TTCGGACTTG GACTTTGAGA AGGTGATGCA CACTCGGAAA 
CGGCACTCGG AACTCTACCA CGAACTCAAC CAGAAGTTCC ACACTTTCGA CCGCTACCGT 
AGCCAGTCCT CAGCCAAGGA GAAACCCAGC CCCCCCGGGG GACGCCCTGG CTTGTCCCAG 
CACAGGAGGC ATCAAAGCTG GAGCACCTTC AAATCTATGA CACTGGGCTC ACTGCCCCCC 
AAGCCCCGAG AACGGCTGGC CCTGCACCGG ACAGCAGCCT GGGAGCCCAC AGAACCGCCA 
GACGGCGACT TCCAGACAGA GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011238811.1
CDS	16..4602
Translation	MTPACPLLLSVILSLRLATAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCTHFAPRLLPLDHYLVNFTCLRPGPEEATARAESEVGRPEEEEEEAAAAASGLELCGGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGANPATTTSPGPPVAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSSSCANGTQQRSRKCSVAGPAWATCAGALTDTRECSNLDCPATDGKWGPWNAWSLCSKTCDTGWQRRFRMCQASGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKRAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDSENKDKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPISAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSSPGKPATPGAATAGSPGRGRGPGTVPPGPGHAHQRLLPADPEESSSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADTNSGDWNTESCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGAPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNILILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGVSDKSKKQRAGASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGGLSFSPLPGNILVPMAASPGLGEPPPPQETNPVYMCGEGGLRQLDLTWIRQSEPGSEGDYMVLPRRTLSLQPGGGGTAGEEAPRARPEGTPRRAAKTVAHTEGYPSFLSVEHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVPEPGERSRTMPRTVPGSTMKLGSLERKKLRYSDLDFEKVMHTRKRHSELYHELNQKFHTFDRYRSQSSAKEKPSPPGGRPGLSQHRRHQSWSTFKSMTLGSLPPKPRERLALHRTAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_011240509.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240509.1

ORF Insert Sequence:

ATGACCCCAG CCTGTCCCCT CTTACTGTCT GTGATTCTGT CCCTGCGCCT GGCCACGGCC 
TTCGACCCTG CCCCCAGTGC CTGCTCTGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTC 
TCGCTGCAGG ACCTCTTTCC CACCATCGCC TCGGGCTGCT CCTGGACCCT GGAGAACCCA 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGGC AGGAGCAGGT TTGCACACAC 
TTTGCCCCGC GCCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTTACCTG CCTGCGGCCT 
GGTCCAGAGG AAGCCACAGC CCGGGCTGAG TCGGAGGTGG GACGGCCAGA GGAGGAGGAG 
GAGGAGGCGG CGGCAGCAGC ATCAGGGTTG GAGTTGTGTG GTGGCTCAGG CCCCTTTACC 
TTTCTGCACT TCGACAAGAA CTTTGTGCAG CTGTGCCTGT CGGCTGAGCC CTCTGAGGCC 
CCTCGTCTGC TAGCGCCTGC TGCCCTGGCC TTCCGTTTTG TGGAGGTCTT GCTGATCAAC 
AACAACAACT CCAGCCAGTT CACCTGTGGT GTGCTCTGCC GCTGGAGTGA GGAGTGTGGC 
CGGGCTGCAG GCAGGGCTTG TGGCTTTGCA CAGCCAGGGT GTAGTTGTCC TGGGGAGGCA 
GGGGCCAACC CCGCCACCAC CACATCTCCG GGGCCTCCGG TTGCCCACAC CCTGTCCAAT 
GCCCTGGTGC CCGGGGGCCC AGCCCCTCCT GCTGAGGCCG ACTTGCACTC GGGGAGCAGC 
AATGACCTGT TCACCACCGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG 
AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTAT ACATGGCGCA GACAGGCGAC 
CCAGCAGCTG AGGAGTGGTC CCCGTGGAGC GTGTGTTCCC TGACGTGTGG GCAGGGTCTG 
CAGGTGCGGA CCCGCTCCTG CGTGTCCTCC CCCTATGGGA CCCTGTGCAG CGGGCCCCTT 
CGGGAGACCC GGCCTTGCAA CAATTCAGCC ACCTGCCCAG TGCACGGCGT GTGGGAGGAG 
TGGGGGTCCT GGAGCCTGTG CTCCCGCAGC TGCGGGCGGG GGTCTCGGAG CCGGATGCGG 
ACCTGCGTGC CCCCCCAGCA CGGCGGCAAG GCCTGCGAGG GTCCCGAGCT GCAGACTAAA 
CTCTGCAGTA TGGCCGCCTG CCCGGTGGAA GGCCAGTGGC TAGAATGGGG TCCCTGGGGC 
CCATGCTCAT CATCTTGTGC CAATGGGACC CAGCAGCGCA GCCGGAAATG CAGTGTGGCG 
GGTCCAGCCT GGGCCACGTG CGCAGGTGCC CTCACGGATA CCCGTGAGTG CAGCAATCTC 
GATTGCCCGG CCACTGACGG CAAGTGGGGG CCGTGGAACG CGTGGAGCCT GTGCTCCAAG 
ACGTGTGACA CGGGCTGGCA ACGCCGCTTC CGCATGTGCC AGGCTTCTGG CACACAGGGC 
TACCCTTGCG AGGGCACAGG AGAGGAGGTG AAACCCTGCA GTGAGAAGAG GTGTCCAGCC 
TTCCATGAGA TGTGCAGGGA TGAGTACGTG ATGTTGATGA CATGGAAGAG GGCGGCAGCT 
GGCGAGATCA TTTACAACAA GTGTCCCCCT AATGCCTCGG GTTCTGCTAG CCGCCGCTGT 
CTCCTCAGTG CCCAGGGCGT AGCATACTGG GGACTGCCCA GCTTTGCTCG TTGCATATCC 
CATGAATACC GCTACCTGTA CCTGTCACTT CGGGAACACC TGGCTAAGGG CCAGCGCATG 
CTGGCAGGTG AGGGCATGTC ACAGGTGGTG CGGAGCCTGC AGGAGCTACT GGCACGGCGC 
ACTTACTACA GCGGGGACCT GCTCTTCTCT GTGGACATCC TAAGGAACGT CACTGACACC 
TTCAAGAGGG CCACCTATGT CCCTTCCGCC GATGACGTGC AGCGTTTCTT CCAGGTGGTG 
AGCTTCATGG TGGATTCAGA AAACAAGGAC AAATGGGATG ATGCTCAGCA GGTGTCACCG 
GGCTCTGTGC ACCTGCTGCG TGTTGTGGAA GATTTCATTC ACCTCGTGGG CGACGCTCTC 
AAGGCCTTCC AGAGCTCTCT CATTGTCACG GACAATCTGG TGATCAGCAT TCAGAGAGAG 
CCTATCTCCG CCGTGTCCAG TGACATCACG TTTCCCATGC GGGGCCGCAG GGGCATGAAG 
GACTGGGTGC GACACTCAGA GGATCGTCTC TTTCTACCCA AGGAGGTGCT CAGCCTGTCC 
TCCCCAGGAA AGCCAGCCAC ACCTGGGGCA GCCACAGCAG GCAGCCCGGG CAGGGGGAGG 
GGCCCAGGAA CGGTGCCCCC TGGCCCAGGC CACGCCCACC AGCGCCTTCT CCCAGCTGAC 
CCCGAAGAGT CCTCCTCCTA CTTTGTGATC GGTGCTGTGC TCTACCGCAC CCTTGGCCTC 
ATCCTGCCGC CCCCCAGGCC TCCACTTGCT GTCACCTCCC GGGTGATGAC AGTGACTGTG 
CGTCCCCCCA CCCAGCCTCC AGCTGAGCCC CTCATTACAG TGGAACTCTC GTACATCATC 
AATGGCACCA CCGATCCCCA CTGTGCCAGC TGGGACTACT CCAGAGCAGA TACCAACTCG 
GGGGACTGGA ACACTGAGAG CTGCCAGACC TTGGAGACCC AGGCGGCTCA CACCCGCTGC 
CAGTGCCAGC ACCTGTCCAC CTTTGCCGTC CTGGCCCAGC CACCCAAGGA CCTGACCCTG 
GAGCTGGCAG GTGCTCCCTC TGTCCCCCTG GTGATCGGCT GTGCAGTGTC CTGCATGGCT 
CTGCTCACCC TGCTGGCCAT CTATGCAGCC TTCTGGAGGT TTATAAAATC AGAACGCTCC 
ATCATCTTGC TGAACTTCTG CCTGTCCATC CTGGCTTCCA ACATTCTGAT CCTGGTGGGC 
CAGTCCCGGG TGCTGAGCAA GGGCGTATGC ACCATGACGG CTGCCTTCCT ACACTTCTTC 
TTTCTGTCCT CCTTTTGCTG GGTGCTTACA GAGGCCTGGC AATCCTATCT GGCTGTCATC 
GGGCGGATGC GCACCCGCCT GGTTCGCAAG CGCTTCCTCT GCCTGGGCTG GGGTCTACCT 
GCCCTGGTGG TGGCTGTGTC TGTTGGCTTT ACTCGCACCA AAGGATATGG TACATCCAGC 
TACTGCTGGC TGTCCCTAGA GGGCGGCCTG CTCTATGCCT TCGTGGGTCC AGCAGCAGTC 
ATTGTCCTGG TGAACATGCT CATCGGGATT ATCGTCTTCA ACAAGCTCAT GGCTCGCGAT 
GGCGTCTCAG ACAAATCTAA GAAGCAGAGG GCTGGGGCTT CACTCTGGAG TTCCTGCGTG 
GTACTGCCTC TCCTGGCGCT TACCTGGATG TCTGCCGTCC TGGCCATGAC AGATCGCCGC 
TCCGTCCTCT TCCAGGCACT CTTTGCCGTT TTCAACTCTG CACAAGGCTT TGTCATCACC 
GCTGTGCACT GCTTCCTGCG CCGAGAGGTC CAGGATGTGG TAAAGTGTCA GATGGGTGTG 
TGTCGGGCTG ATGAGAGTGA AGACTCCCCA GACTCGTGCA AGAACGGGCA GCTGCAGATC 
CTGTCAGACT TTGAAAAGGA CGTGGATCTG GCTTGTCAGA CAGTTCTGTT CAAGGAGGTC 
AACACCTGCA ACCCGTCCAC CATTACCGGC ACTCTGTCCC GCCTGTCTCT GGATGAGGAT 
GAGGAGCCCA AGTCCTGTCT CGTGGGTCCT GAGGGTGGCC TCAGCTTCTC ACCACTGCCT 
GGGAACATCC TGGTACCCAT GGCAGCCTCA CCAGGTCTAG GGGAGCCACC ACCACCCCAG 
GAGACCAACC CTGTGTACAT GTGTGGGGAG GGTGGCCTGC GGCAGTTGGA CCTTACATGG 
ATACGGCAGA GTGAACCAGG CTCTGAGGGG GACTACATGG TTCTGCCCCG GCGGACTTTG 
AGCCTGCAGC CTGGTGGTGG GGGTACAGCG GGTGAGGAGG CCCCAAGGGC CCGGCCTGAG 
GGGACCCCCC GGCGGGCTGC CAAAACGGTA GCCCACACTG AAGGCTACCC CAGCTTCCTG 
TCTGTGGAGC ACTCGGGTCT AGGGCTGGGC CCTGCCTATG GGTCTCTCCA GAACCCGTAT 
GGAATGACCT TCCAACCACC ACCACCAACA CCCAGCGCCC GCCAAGTACC AGAGCCAGGA 
GAACGTAGCC GGACCATGCC CCGTACAGTG CCTGGTTCCA CCATGAAGCT GGGCTCCCTT 
GAGCGAAAGA AGCTTCGGTA TTCGGACTTG GACTTTGAGA AGGTGATGCA CACTCGGAAA 
CGGCACTCGG AACTCTACCA CGAACTCAAC CAGAAGTTCC ACACTTTCGA CCGCTACCGT 
AGCCAGTCCT CAGCCAAGGA GAAACCCAGC CCCCCCGGGG GACGCCCTGG CTTGTCCCAG 
CACAGGAGGC ATCAAAGCTG GAGCACCTTC AAATCTATGA CACTGGGCTC ACTGCCCCCC 
AAGCCCCGAG AACGGCTGGC CCTGCACCGG ACAGCAGCCT GGGAGCCCAC AGAACCGCCA 
GACGGCGACT TCCAGACAGA GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adgrb2 ( XM_011240510.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011238812.1 Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant X6, mRNA.

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CloneID	OMu73517
Clone ID Related Accession (Same CDS sequence)	XM_011240510.1 , XM_030253457.1
Accession Version	XM_011240510.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4584bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	adhesion G protein-coupled receptor B2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCAG CCTGTCCCCT CTTACTGTCT GTGATTCTGT CCCTGCGCCT GGCCACGGCC 
TTCGACCCTG CCCCCAGTGC CTGCTCTGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTC 
TCGCTGCAGG ACCTCTTTCC CACCATCGCC TCGGGCTGCT CCTGGACCCT GGAGAACCCA 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGGC AGGAGCAGGT TTGCACACAC 
TTTGCCCCGC GCCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTTACCTG CCTGCGGCCT 
GGTCCAGAGG AAGCCACAGC CCGGGCTGAG TCGGAGGTGG GACGGCCAGA GGAGGAGGAG 
GAGGAGGCGG CGGCAGCAGC ATCAGGGTTG GAGTTGTGTG GTGGCTCAGG CCCCTTTACC 
TTTCTGCACT TCGACAAGAA CTTTGTGCAG CTGTGCCTGT CGGCTGAGCC CTCTGAGGCC 
CCTCGTCTGC TAGCGCCTGC TGCCCTGGCC TTCCGTTTTG TGGAGGTCTT GCTGATCAAC 
AACAACAACT CCAGCCAGTT CACCTGTGGT GTGCTCTGCC GCTGGAGTGA GGAGTGTGGC 
CGGGCTGCAG GCAGGGCTTG TGGCTTTGCA CAGCCAGGGT GTAGTTGTCC TGGGGAGGCA 
GGGGCCAACC CCGCCACCAC CACATCTCCG GGGCCTCCGG TTGCCCACAC CCTGTCCAAT 
GCCCTGGTGC CCGGGGGCCC AGCCCCTCCT GCTGAGGCCG ACTTGCACTC GGGGAGCAGC 
AATGACCTGT TCACCACCGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG 
AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTAT ACATGGCGCA GACAGGCGAC 
CCAGCAGCTG AGGAGTGGTC CCCGTGGAGC GTGTGTTCCC TGACGTGTGG GCAGGGTCTG 
CAGGTGCGGA CCCGCTCCTG CGTGTCCTCC CCCTATGGGA CCCTGTGCAG CGGGCCCCTT 
CGGGAGACCC GGCCTTGCAA CAATTCAGCC ACCTGCCCAG TGGAAGGCCA GTGGCTAGAA 
TGGGGTCCCT GGGGCCCATG CTCATCATCT TGTGCCAATG GGACCCAGCA GCGCAGCCGG 
AAATGCAGTG TGGCGGGTCC AGCCTGGGCC ACGTGCGCAG GTGCCCTCAC GGATACCCGT 
GAGTGCAGCA ATCTCGATTG CCCGGCCACT GACGGCAAGT GGGGGCCGTG GAACGCGTGG 
AGCCTGTGCT CCAAGACGTG TGACACGGGC TGGCAACGCC GCTTCCGCAT GTGCCAGGCT 
TCTGGCACAC AGGGCTACCC TTGCGAGGGC ACAGGAGAGG AGGTGAAACC CTGCAGTGAG 
AAGAGGTGTC CAGCCTTCCA TGAGATGTGC AGGGATGAGT ACGTGATGTT GATGACATGG 
AAGAGGGCGG CAGCTGGCGA GATCATTTAC AACAAGTGTC CCCCTAATGC CTCGGGTTCT 
GCTAGCCGCC GCTGTCTCCT CAGTGCCCAG GGCGTAGCAT ACTGGGGACT GCCCAGCTTT 
GCTCGTTGCA TATCCCATGA ATACCGCTAC CTGTACCTGT CACTTCGGGA ACACCTGGCT 
AAGGGCCAGC GCATGCTGGC AGGTGAGGGC ATGTCACAGG TGGTGCGGAG CCTGCAGGAG 
CTACTGGCAC GGCGCACTTA CTACAGCGGG GACCTGCTCT TCTCTGTGGA CATCCTAAGG 
AACGTCACTG ACACCTTCAA GAGGGCCACC TATGTCCCTT CCGCCGATGA CGTGCAGCGT 
TTCTTCCAGG TGGTGAGCTT CATGGTGGAT TCAGAAAACA AGGACAAATG GGATGATGCT 
CAGCAGGTGT CACCGGGCTC TGTGCACCTG CTGCGTGTTG TGGAAGATTT CATTCACCTC 
GTGGGCGACG CTCTCAAGGC CTTCCAGAGC TCTCTCATTG TCACGGACAA TCTGGTGATC 
AGCATTCAGA GAGAGCCTAT CTCCGCCGTG TCCAGTGACA TCACGTTTCC CATGCGGGGC 
CGCAGGGGCA TGAAGGACTG GGTGCGACAC TCAGAGGATC GTCTCTTTCT ACCCAAGGAG 
GTGCTCAGCC TGTCCTCCCC AGGAAAGCCA GCCACACCTG GGGCAGCCAC AGCAGGCAGC 
CCGGGCAGGG GGAGGGGCCC AGGAACGGTG CCCCCTGGCC CAGGCCACGC CCACCAGCGC 
CTTCTCCCAG CTGACCCCGA AGAGTCCTCC TCCTACTTTG TGATCGGTGC TGTGCTCTAC 
CGCACCCTTG GCCTCATCCT GCCGCCCCCC AGGCCTCCAC TTGCTGTCAC CTCCCGGGTG 
ATGACAGTGA CTGTGCGTCC CCCCACCCAG CCTCCAGCTG AGCCCCTCAT TACAGTGGAA 
CTCTCGTACA TCATCAATGG CACCACCGAT CCCCACTGTG CCAGCTGGGA CTACTCCAGA 
GCAGATACCA ACTCGGGGGA CTGGAACACT GAGAGCTGCC AGACCTTGGA GACCCAGGCG 
GCTCACACCC GCTGCCAGTG CCAGCACCTG TCCACCTTTG CCGTCCTGGC CCAGCCACCC 
AAGGACCTGA CCCTGGAGCT GGCAGGTGCT CCCTCTGTCC CCCTGGTGAT CGGCTGTGCA 
GTGTCCTGCA TGGCTCTGCT CACCCTGCTG GCCATCTATG CAGCCTTCTG GAGGTTTATA 
AAATCAGAAC GCTCCATCAT CTTGCTGAAC TTCTGCCTGT CCATCCTGGC TTCCAACATT 
CTGATCCTGG TGGGCCAGTC CCGGGTGCTG AGCAAGGGCG TATGCACCAT GACGGCTGCC 
TTCCTACACT TCTTCTTTCT GTCCTCCTTT TGCTGGGTGC TTACAGAGGC CTGGCAATCC 
TATCTGGCTG TCATCGGGCG GATGCGCACC CGCCTGGTTC GCAAGCGCTT CCTCTGCCTG 
GGCTGGGGTC TACCTGCCCT GGTGGTGGCT GTGTCTGTTG GCTTTACTCG CACCAAAGGA 
TATGGTACAT CCAGCTACTG CTGGCTGTCC CTAGAGGGCG GCCTGCTCTA TGCCTTCGTG 
GGTCCAGCAG CAGTCATTGT CCTGGTGAAC ATGCTCATCG GGATTATCGT CTTCAACAAG 
CTCATGGCTC GCGATGGCGT CTCAGACAAA TCTAAGAAGC AGAGGGCTGG GTCGGAGCGG 
TGCCCCTGGG CCAGCCTGCT CCTTCCCTGC TCAGCGTGTG GAGCGGTCCC CAGCCCCCTG 
CTCAGCTCAG CCTCGGCCAG GAATGCCATC CGACTGTCTT GGAATCTCTG GGGATATGGC 
TCTCAACTGT GTCTTTTTCC AAAGCTTCCA AGGGCTTCAC TCTGGAGTTC CTGCGTGGTA 
CTGCCTCTCC TGGCGCTTAC CTGGATGTCT GCCGTCCTGG CCATGACAGA TCGCCGCTCC 
GTCCTCTTCC AGGCACTCTT TGCCGTTTTC AACTCTGCAC AAGGCTTTGT CATCACCGCT 
GTGCACTGCT TCCTGCGCCG AGAGGTCCAG GATGTGGTAA AGTGTCAGAT GGGTGTGTGT 
CGGGCTGATG AGAGTGAAGA CTCCCCAGAC TCGTGCAAGA ACGGGCAGCT GCAGATCCTG 
TCAGACTTTG AAAAGGACGT GGATCTGGCT TGTCAGACAG TTCTGTTCAA GGAGGTCAAC 
ACCTGCAACC CGTCCACCAT TACCGGCACT CTGTCCCGCC TGTCTCTGGA TGAGGATGAG 
GAGCCCAAGT CCTGTCTCGT GGGTCCTGAG GGTGGCCTCA GCTTCTCACC ACTGCCTGGG 
AACATCCTGG TACCCATGGC AGCCTCACCA GGTCTAGGGG AGCCACCACC ACCCCAGGAG 
ACCAACCCTG TGTACATGTG TGGGGAGGGT GGCCTGCGGC AGTTGGACCT TACATGGATA 
CGGCAGAGTG AACCAGGCTC TGAGGGGGAC TACATGGTTC TGCCCCGGCG GACTTTGAGC 
CTGCAGCCTG GTGGTGGGGG TACAGCGGGT GAGGAGGCCC CAAGGGCCCG GCCTGAGGGG 
ACCCCCCGGC GGGCTGCCAA AACGGTAGCC CACACTGAAG GCTACCCCAG CTTCCTGTCT 
GTGGAGCACT CGGGTCTAGG GCTGGGCCCT GCCTATGGGT CTCTCCAGAA CCCGTATGGA 
ATGACCTTCC AACCACCACC ACCAACACCC AGCGCCCGCC AAGTACCAGA GCCAGGAGAA 
CGTAGCCGGA CCATGCCCCG TACAGTGCCT GGTTCCACCA TGAAGCTGGG CTCCCTTGAG 
CGAAAGAAGC TTCGGTATTC GGACTTGGAC TTTGAGAAGG TGATGCACAC TCGGAAACGG 
CACTCGGAAC TCTACCACGA ACTCAACCAG AAGTTCCACA CTTTCGACCG CTACCGTAGC 
CAGTCCTCAG CCAAGGAGAA ACCCAGCCCC CCCGGGGGAC GCCCTGGCTT GTCCCAGCAC 
AGGAGGCATC AAAGCTGGAG CACCTTCAAA TCTATGACAC TGGGCTCACT GCCCCCCAAG 
CCCCGAGAAC GGCTGGCCCT GCACCGGACA GCAGCCTGGG AGCCCACAGA ACCGCCAGAC 
GGCGACTTCC AGACAGAGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011238812.1
CDS	16..4599
Translation	MTPACPLLLSVILSLRLATAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCTHFAPRLLPLDHYLVNFTCLRPGPEEATARAESEVGRPEEEEEEAAAAASGLELCGGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGANPATTTSPGPPVAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVEGQWLEWGPWGPCSSSCANGTQQRSRKCSVAGPAWATCAGALTDTRECSNLDCPATDGKWGPWNAWSLCSKTCDTGWQRRFRMCQASGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKRAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDSENKDKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPISAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSSPGKPATPGAATAGSPGRGRGPGTVPPGPGHAHQRLLPADPEESSSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADTNSGDWNTESCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGAPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNILILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGVSDKSKKQRAGSERCPWASLLLPCSACGAVPSPLLSSASARNAIRLSWNLWGYGSQLCLFPKLPRASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGGLSFSPLPGNILVPMAASPGLGEPPPPQETNPVYMCGEGGLRQLDLTWIRQSEPGSEGDYMVLPRRTLSLQPGGGGTAGEEAPRARPEGTPRRAAKTVAHTEGYPSFLSVEHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVPEPGERSRTMPRTVPGSTMKLGSLERKKLRYSDLDFEKVMHTRKRHSELYHELNQKFHTFDRYRSQSSAKEKPSPPGGRPGLSQHRRHQSWSTFKSMTLGSLPPKPRERLALHRTAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_011240510.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240510.1

ORF Insert Sequence:

ATGACCCCAG CCTGTCCCCT CTTACTGTCT GTGATTCTGT CCCTGCGCCT GGCCACGGCC 
TTCGACCCTG CCCCCAGTGC CTGCTCTGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTC 
TCGCTGCAGG ACCTCTTTCC CACCATCGCC TCGGGCTGCT CCTGGACCCT GGAGAACCCA 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGGC AGGAGCAGGT TTGCACACAC 
TTTGCCCCGC GCCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTTACCTG CCTGCGGCCT 
GGTCCAGAGG AAGCCACAGC CCGGGCTGAG TCGGAGGTGG GACGGCCAGA GGAGGAGGAG 
GAGGAGGCGG CGGCAGCAGC ATCAGGGTTG GAGTTGTGTG GTGGCTCAGG CCCCTTTACC 
TTTCTGCACT TCGACAAGAA CTTTGTGCAG CTGTGCCTGT CGGCTGAGCC CTCTGAGGCC 
CCTCGTCTGC TAGCGCCTGC TGCCCTGGCC TTCCGTTTTG TGGAGGTCTT GCTGATCAAC 
AACAACAACT CCAGCCAGTT CACCTGTGGT GTGCTCTGCC GCTGGAGTGA GGAGTGTGGC 
CGGGCTGCAG GCAGGGCTTG TGGCTTTGCA CAGCCAGGGT GTAGTTGTCC TGGGGAGGCA 
GGGGCCAACC CCGCCACCAC CACATCTCCG GGGCCTCCGG TTGCCCACAC CCTGTCCAAT 
GCCCTGGTGC CCGGGGGCCC AGCCCCTCCT GCTGAGGCCG ACTTGCACTC GGGGAGCAGC 
AATGACCTGT TCACCACCGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG 
AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTAT ACATGGCGCA GACAGGCGAC 
CCAGCAGCTG AGGAGTGGTC CCCGTGGAGC GTGTGTTCCC TGACGTGTGG GCAGGGTCTG 
CAGGTGCGGA CCCGCTCCTG CGTGTCCTCC CCCTATGGGA CCCTGTGCAG CGGGCCCCTT 
CGGGAGACCC GGCCTTGCAA CAATTCAGCC ACCTGCCCAG TGGAAGGCCA GTGGCTAGAA 
TGGGGTCCCT GGGGCCCATG CTCATCATCT TGTGCCAATG GGACCCAGCA GCGCAGCCGG 
AAATGCAGTG TGGCGGGTCC AGCCTGGGCC ACGTGCGCAG GTGCCCTCAC GGATACCCGT 
GAGTGCAGCA ATCTCGATTG CCCGGCCACT GACGGCAAGT GGGGGCCGTG GAACGCGTGG 
AGCCTGTGCT CCAAGACGTG TGACACGGGC TGGCAACGCC GCTTCCGCAT GTGCCAGGCT 
TCTGGCACAC AGGGCTACCC TTGCGAGGGC ACAGGAGAGG AGGTGAAACC CTGCAGTGAG 
AAGAGGTGTC CAGCCTTCCA TGAGATGTGC AGGGATGAGT ACGTGATGTT GATGACATGG 
AAGAGGGCGG CAGCTGGCGA GATCATTTAC AACAAGTGTC CCCCTAATGC CTCGGGTTCT 
GCTAGCCGCC GCTGTCTCCT CAGTGCCCAG GGCGTAGCAT ACTGGGGACT GCCCAGCTTT 
GCTCGTTGCA TATCCCATGA ATACCGCTAC CTGTACCTGT CACTTCGGGA ACACCTGGCT 
AAGGGCCAGC GCATGCTGGC AGGTGAGGGC ATGTCACAGG TGGTGCGGAG CCTGCAGGAG 
CTACTGGCAC GGCGCACTTA CTACAGCGGG GACCTGCTCT TCTCTGTGGA CATCCTAAGG 
AACGTCACTG ACACCTTCAA GAGGGCCACC TATGTCCCTT CCGCCGATGA CGTGCAGCGT 
TTCTTCCAGG TGGTGAGCTT CATGGTGGAT TCAGAAAACA AGGACAAATG GGATGATGCT 
CAGCAGGTGT CACCGGGCTC TGTGCACCTG CTGCGTGTTG TGGAAGATTT CATTCACCTC 
GTGGGCGACG CTCTCAAGGC CTTCCAGAGC TCTCTCATTG TCACGGACAA TCTGGTGATC 
AGCATTCAGA GAGAGCCTAT CTCCGCCGTG TCCAGTGACA TCACGTTTCC CATGCGGGGC 
CGCAGGGGCA TGAAGGACTG GGTGCGACAC TCAGAGGATC GTCTCTTTCT ACCCAAGGAG 
GTGCTCAGCC TGTCCTCCCC AGGAAAGCCA GCCACACCTG GGGCAGCCAC AGCAGGCAGC 
CCGGGCAGGG GGAGGGGCCC AGGAACGGTG CCCCCTGGCC CAGGCCACGC CCACCAGCGC 
CTTCTCCCAG CTGACCCCGA AGAGTCCTCC TCCTACTTTG TGATCGGTGC TGTGCTCTAC 
CGCACCCTTG GCCTCATCCT GCCGCCCCCC AGGCCTCCAC TTGCTGTCAC CTCCCGGGTG 
ATGACAGTGA CTGTGCGTCC CCCCACCCAG CCTCCAGCTG AGCCCCTCAT TACAGTGGAA 
CTCTCGTACA TCATCAATGG CACCACCGAT CCCCACTGTG CCAGCTGGGA CTACTCCAGA 
GCAGATACCA ACTCGGGGGA CTGGAACACT GAGAGCTGCC AGACCTTGGA GACCCAGGCG 
GCTCACACCC GCTGCCAGTG CCAGCACCTG TCCACCTTTG CCGTCCTGGC CCAGCCACCC 
AAGGACCTGA CCCTGGAGCT GGCAGGTGCT CCCTCTGTCC CCCTGGTGAT CGGCTGTGCA 
GTGTCCTGCA TGGCTCTGCT CACCCTGCTG GCCATCTATG CAGCCTTCTG GAGGTTTATA 
AAATCAGAAC GCTCCATCAT CTTGCTGAAC TTCTGCCTGT CCATCCTGGC TTCCAACATT 
CTGATCCTGG TGGGCCAGTC CCGGGTGCTG AGCAAGGGCG TATGCACCAT GACGGCTGCC 
TTCCTACACT TCTTCTTTCT GTCCTCCTTT TGCTGGGTGC TTACAGAGGC CTGGCAATCC 
TATCTGGCTG TCATCGGGCG GATGCGCACC CGCCTGGTTC GCAAGCGCTT CCTCTGCCTG 
GGCTGGGGTC TACCTGCCCT GGTGGTGGCT GTGTCTGTTG GCTTTACTCG CACCAAAGGA 
TATGGTACAT CCAGCTACTG CTGGCTGTCC CTAGAGGGCG GCCTGCTCTA TGCCTTCGTG 
GGTCCAGCAG CAGTCATTGT CCTGGTGAAC ATGCTCATCG GGATTATCGT CTTCAACAAG 
CTCATGGCTC GCGATGGCGT CTCAGACAAA TCTAAGAAGC AGAGGGCTGG GTCGGAGCGG 
TGCCCCTGGG CCAGCCTGCT CCTTCCCTGC TCAGCGTGTG GAGCGGTCCC CAGCCCCCTG 
CTCAGCTCAG CCTCGGCCAG GAATGCCATC CGACTGTCTT GGAATCTCTG GGGATATGGC 
TCTCAACTGT GTCTTTTTCC AAAGCTTCCA AGGGCTTCAC TCTGGAGTTC CTGCGTGGTA 
CTGCCTCTCC TGGCGCTTAC CTGGATGTCT GCCGTCCTGG CCATGACAGA TCGCCGCTCC 
GTCCTCTTCC AGGCACTCTT TGCCGTTTTC AACTCTGCAC AAGGCTTTGT CATCACCGCT 
GTGCACTGCT TCCTGCGCCG AGAGGTCCAG GATGTGGTAA AGTGTCAGAT GGGTGTGTGT 
CGGGCTGATG AGAGTGAAGA CTCCCCAGAC TCGTGCAAGA ACGGGCAGCT GCAGATCCTG 
TCAGACTTTG AAAAGGACGT GGATCTGGCT TGTCAGACAG TTCTGTTCAA GGAGGTCAAC 
ACCTGCAACC CGTCCACCAT TACCGGCACT CTGTCCCGCC TGTCTCTGGA TGAGGATGAG 
GAGCCCAAGT CCTGTCTCGT GGGTCCTGAG GGTGGCCTCA GCTTCTCACC ACTGCCTGGG 
AACATCCTGG TACCCATGGC AGCCTCACCA GGTCTAGGGG AGCCACCACC ACCCCAGGAG 
ACCAACCCTG TGTACATGTG TGGGGAGGGT GGCCTGCGGC AGTTGGACCT TACATGGATA 
CGGCAGAGTG AACCAGGCTC TGAGGGGGAC TACATGGTTC TGCCCCGGCG GACTTTGAGC 
CTGCAGCCTG GTGGTGGGGG TACAGCGGGT GAGGAGGCCC CAAGGGCCCG GCCTGAGGGG 
ACCCCCCGGC GGGCTGCCAA AACGGTAGCC CACACTGAAG GCTACCCCAG CTTCCTGTCT 
GTGGAGCACT CGGGTCTAGG GCTGGGCCCT GCCTATGGGT CTCTCCAGAA CCCGTATGGA 
ATGACCTTCC AACCACCACC ACCAACACCC AGCGCCCGCC AAGTACCAGA GCCAGGAGAA 
CGTAGCCGGA CCATGCCCCG TACAGTGCCT GGTTCCACCA TGAAGCTGGG CTCCCTTGAG 
CGAAAGAAGC TTCGGTATTC GGACTTGGAC TTTGAGAAGG TGATGCACAC TCGGAAACGG 
CACTCGGAAC TCTACCACGA ACTCAACCAG AAGTTCCACA CTTTCGACCG CTACCGTAGC 
CAGTCCTCAG CCAAGGAGAA ACCCAGCCCC CCCGGGGGAC GCCCTGGCTT GTCCCAGCAC 
AGGAGGCATC AAAGCTGGAG CACCTTCAAA TCTATGACAC TGGGCTCACT GCCCCCCAAG 
CCCCGAGAAC GGCTGGCCCT GCACCGGACA GCAGCCTGGG AGCCCACAGA ACCGCCAGAC 
GGCGACTTCC AGACAGAGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adgrb2 ( XM_011240511.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011238813.1 Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu73518
Clone ID Related Accession (Same CDS sequence)	XM_030253456.1 , XM_011240511.1
Accession Version	XM_011240511.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4584bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	adhesion G protein-coupled receptor B2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCAG CCTGTCCCCT CTTACTGTCT GTGATTCTGT CCCTGCGCCT GGCCACGGCC 
TTCGACCCTG CCCCCAGTGC CTGCTCTGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTC 
TCGCTGCAGG ACCTCTTTCC CACCATCGCC TCGGGCTGCT CCTGGACCCT GGAGAACCCA 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGGC AGGAGCAGGT TTGCACACAC 
TTTGCCCCGC GCCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTTACCTG CCTGCGGCCT 
GGTCCAGAGG AAGCCACAGC CCGGGCTGAG TCGGAGGTGG GACGGCCAGA GGAGGAGGAG 
GAGGAGGCGG CGGCAGCAGC ATCAGGGTTG GAGTTGTGTG GTGGCTCAGG CCCCTTTACC 
TTTCTGCACT TCGACAAGAA CTTTGTGCAG CTGTGCCTGT CGGCTGAGCC CTCTGAGGCC 
CCTCGTCTGC TAGCGCCTGC TGCCCTGGCC TTCCGTTTTG TGGAGGTCTT GCTGATCAAC 
AACAACAACT CCAGCCAGTT CACCTGTGGT GTGCTCTGCC GCTGGAGTGA GGAGTGTGGC 
CGGGCTGCAG GCAGGGCTTG TGGCTTTGCA CAGCCAGGGT GTAGTTGTCC TGGGGAGGCA 
GGGGCCAACC CCGCCACCAC CACATCTCCG GGGCCTCCGG TTGCCCACAC CCTGTCCAAT 
GCCCTGGTGC CCGGGGGCCC AGCCCCTCCT GCTGAGGCCG ACTTGCACTC GGGGAGCAGC 
AATGACCTGT TCACCACCGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG 
AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTAT ACATGGCGCA GACAGTGCAC 
GGCGTGTGGG AGGAGTGGGG GTCCTGGAGC CTGTGCTCCC GCAGCTGCGG GCGGGGGTCT 
CGGAGCCGGA TGCGGACCTG CGTGCCCCCC CAGCACGGCG GCAAGGCCTG CGAGGGTCCC 
GAGCTGCAGA CTAAACTCTG CAGTATGGCC GCCTGCCCGG TGGAAGGCCA GTGGCTAGAA 
TGGGGTCCCT GGGGCCCATG CTCATCATCT TGTGCCAATG GGACCCAGCA GCGCAGCCGG 
AAATGCAGTG TGGCGGGTCC AGCCTGGGCC ACGTGCGCAG GTGCCCTCAC GGATACCCGT 
GAGTGCAGCA ATCTCGATTG CCCGGCCACT GACGGCAAGT GGGGGCCGTG GAACGCGTGG 
AGCCTGTGCT CCAAGACGTG TGACACGGGC TGGCAACGCC GCTTCCGCAT GTGCCAGGCT 
TCTGGCACAC AGGGCTACCC TTGCGAGGGC ACAGGAGAGG AGGTGAAACC CTGCAGTGAG 
AAGAGGTGTC CAGCCTTCCA TGAGATGTGC AGGGATGAGT ACGTGATGTT GATGACATGG 
AAGAGGGCGG CAGCTGGCGA GATCATTTAC AACAAGTGTC CCCCTAATGC CTCGGGTTCT 
GCTAGCCGCC GCTGTCTCCT CAGTGCCCAG GGCGTAGCAT ACTGGGGACT GCCCAGCTTT 
GCTCGTTGCA TATCCCATGA ATACCGCTAC CTGTACCTGT CACTTCGGGA ACACCTGGCT 
AAGGGCCAGC GCATGCTGGC AGGTGAGGGC ATGTCACAGG TGGTGCGGAG CCTGCAGGAG 
CTACTGGCAC GGCGCACTTA CTACAGCGGG GACCTGCTCT TCTCTGTGGA CATCCTAAGG 
AACGTCACTG ACACCTTCAA GAGGGCCACC TATGTCCCTT CCGCCGATGA CGTGCAGCGT 
TTCTTCCAGG TGGTGAGCTT CATGGTGGAT TCAGAAAACA AGGACAAATG GGATGATGCT 
CAGCAGGTGT CACCGGGCTC TGTGCACCTG CTGCGTGTTG TGGAAGATTT CATTCACCTC 
GTGGGCGACG CTCTCAAGGC CTTCCAGAGC TCTCTCATTG TCACGGACAA TCTGGTGATC 
AGCATTCAGA GAGAGCCTAT CTCCGCCGTG TCCAGTGACA TCACGTTTCC CATGCGGGGC 
CGCAGGGGCA TGAAGGACTG GGTGCGACAC TCAGAGGATC GTCTCTTTCT ACCCAAGGAG 
GTGCTCAGCC TGTCCTCCCC AGGAAAGCCA GCCACACCTG GGGCAGCCAC AGCAGGCAGC 
CCGGGCAGGG GGAGGGGCCC AGGAACGGTG CCCCCTGGCC CAGGCCACGC CCACCAGCGC 
CTTCTCCCAG CTGACCCCGA AGAGTCCTCC TCCTACTTTG TGATCGGTGC TGTGCTCTAC 
CGCACCCTTG GCCTCATCCT GCCGCCCCCC AGGCCTCCAC TTGCTGTCAC CTCCCGGGTG 
ATGACAGTGA CTGTGCGTCC CCCCACCCAG CCTCCAGCTG AGCCCCTCAT TACAGTGGAA 
CTCTCGTACA TCATCAATGG CACCACCGAT CCCCACTGTG CCAGCTGGGA CTACTCCAGA 
GCAGATACCA ACTCGGGGGA CTGGAACACT GAGAGCTGCC AGACCTTGGA GACCCAGGCG 
GCTCACACCC GCTGCCAGTG CCAGCACCTG TCCACCTTTG CCGTCCTGGC CCAGCCACCC 
AAGGACCTGA CCCTGGAGCT GGCAGGTGCT CCCTCTGTCC CCCTGGTGAT CGGCTGTGCA 
GTGTCCTGCA TGGCTCTGCT CACCCTGCTG GCCATCTATG CAGCCTTCTG GAGGTTTATA 
AAATCAGAAC GCTCCATCAT CTTGCTGAAC TTCTGCCTGT CCATCCTGGC TTCCAACATT 
CTGATCCTGG TGGGCCAGTC CCGGGTGCTG AGCAAGGGCG TATGCACCAT GACGGCTGCC 
TTCCTACACT TCTTCTTTCT GTCCTCCTTT TGCTGGGTGC TTACAGAGGC CTGGCAATCC 
TATCTGGCTG TCATCGGGCG GATGCGCACC CGCCTGGTTC GCAAGCGCTT CCTCTGCCTG 
GGCTGGGGTC TACCTGCCCT GGTGGTGGCT GTGTCTGTTG GCTTTACTCG CACCAAAGGA 
TATGGTACAT CCAGCTACTG CTGGCTGTCC CTAGAGGGCG GCCTGCTCTA TGCCTTCGTG 
GGTCCAGCAG CAGTCATTGT CCTGGTGAAC ATGCTCATCG GGATTATCGT CTTCAACAAG 
CTCATGGCTC GCGATGGCGT CTCAGACAAA TCTAAGAAGC AGAGGGCTGG GTCGGAGCGG 
TGCCCCTGGG CCAGCCTGCT CCTTCCCTGC TCAGCGTGTG GAGCGGTCCC CAGCCCCCTG 
CTCAGCTCAG CCTCGGCCAG GAATGCCATC CGACTGTCTT GGAATCTCTG GGGATATGGC 
TCTCAACTGT GTCTTTTTCC AAAGCTTCCA AGGGCTTCAC TCTGGAGTTC CTGCGTGGTA 
CTGCCTCTCC TGGCGCTTAC CTGGATGTCT GCCGTCCTGG CCATGACAGA TCGCCGCTCC 
GTCCTCTTCC AGGCACTCTT TGCCGTTTTC AACTCTGCAC AAGGCTTTGT CATCACCGCT 
GTGCACTGCT TCCTGCGCCG AGAGGTCCAG GATGTGGTAA AGTGTCAGAT GGGTGTGTGT 
CGGGCTGATG AGAGTGAAGA CTCCCCAGAC TCGTGCAAGA ACGGGCAGCT GCAGATCCTG 
TCAGACTTTG AAAAGGACGT GGATCTGGCT TGTCAGACAG TTCTGTTCAA GGAGGTCAAC 
ACCTGCAACC CGTCCACCAT TACCGGCACT CTGTCCCGCC TGTCTCTGGA TGAGGATGAG 
GAGCCCAAGT CCTGTCTCGT GGGTCCTGAG GGTGGCCTCA GCTTCTCACC ACTGCCTGGG 
AACATCCTGG TACCCATGGC AGCCTCACCA GGTCTAGGGG AGCCACCACC ACCCCAGGAG 
ACCAACCCTG TGTACATGTG TGGGGAGGGT GGCCTGCGGC AGTTGGACCT TACATGGATA 
CGGCAGAGTG AACCAGGCTC TGAGGGGGAC TACATGGTTC TGCCCCGGCG GACTTTGAGC 
CTGCAGCCTG GTGGTGGGGG TACAGCGGGT GAGGAGGCCC CAAGGGCCCG GCCTGAGGGG 
ACCCCCCGGC GGGCTGCCAA AACGGTAGCC CACACTGAAG GCTACCCCAG CTTCCTGTCT 
GTGGAGCACT CGGGTCTAGG GCTGGGCCCT GCCTATGGGT CTCTCCAGAA CCCGTATGGA 
ATGACCTTCC AACCACCACC ACCAACACCC AGCGCCCGCC AAGTACCAGA GCCAGGAGAA 
CGTAGCCGGA CCATGCCCCG TACAGTGCCT GGTTCCACCA TGAAGCTGGG CTCCCTTGAG 
CGAAAGAAGC TTCGGTATTC GGACTTGGAC TTTGAGAAGG TGATGCACAC TCGGAAACGG 
CACTCGGAAC TCTACCACGA ACTCAACCAG AAGTTCCACA CTTTCGACCG CTACCGTAGC 
CAGTCCTCAG CCAAGGAGAA ACCCAGCCCC CCCGGGGGAC GCCCTGGCTT GTCCCAGCAC 
AGGAGGCATC AAAGCTGGAG CACCTTCAAA TCTATGACAC TGGGCTCACT GCCCCCCAAG 
CCCCGAGAAC GGCTGGCCCT GCACCGGACA GCAGCCTGGG AGCCCACAGA ACCGCCAGAC 
GGCGACTTCC AGACAGAGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011238813.1
CDS	16..4599
Translation	MTPACPLLLSVILSLRLATAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCTHFAPRLLPLDHYLVNFTCLRPGPEEATARAESEVGRPEEEEEEAAAAASGLELCGGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGANPATTTSPGPPVAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSSSCANGTQQRSRKCSVAGPAWATCAGALTDTRECSNLDCPATDGKWGPWNAWSLCSKTCDTGWQRRFRMCQASGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKRAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDSENKDKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPISAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSSPGKPATPGAATAGSPGRGRGPGTVPPGPGHAHQRLLPADPEESSSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADTNSGDWNTESCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGAPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNILILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGVSDKSKKQRAGSERCPWASLLLPCSACGAVPSPLLSSASARNAIRLSWNLWGYGSQLCLFPKLPRASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGGLSFSPLPGNILVPMAASPGLGEPPPPQETNPVYMCGEGGLRQLDLTWIRQSEPGSEGDYMVLPRRTLSLQPGGGGTAGEEAPRARPEGTPRRAAKTVAHTEGYPSFLSVEHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVPEPGERSRTMPRTVPGSTMKLGSLERKKLRYSDLDFEKVMHTRKRHSELYHELNQKFHTFDRYRSQSSAKEKPSPPGGRPGLSQHRRHQSWSTFKSMTLGSLPPKPRERLALHRTAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_011240511.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240511.1

ORF Insert Sequence:

ATGACCCCAG CCTGTCCCCT CTTACTGTCT GTGATTCTGT CCCTGCGCCT GGCCACGGCC 
TTCGACCCTG CCCCCAGTGC CTGCTCTGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTC 
TCGCTGCAGG ACCTCTTTCC CACCATCGCC TCGGGCTGCT CCTGGACCCT GGAGAACCCA 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGGC AGGAGCAGGT TTGCACACAC 
TTTGCCCCGC GCCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTTACCTG CCTGCGGCCT 
GGTCCAGAGG AAGCCACAGC CCGGGCTGAG TCGGAGGTGG GACGGCCAGA GGAGGAGGAG 
GAGGAGGCGG CGGCAGCAGC ATCAGGGTTG GAGTTGTGTG GTGGCTCAGG CCCCTTTACC 
TTTCTGCACT TCGACAAGAA CTTTGTGCAG CTGTGCCTGT CGGCTGAGCC CTCTGAGGCC 
CCTCGTCTGC TAGCGCCTGC TGCCCTGGCC TTCCGTTTTG TGGAGGTCTT GCTGATCAAC 
AACAACAACT CCAGCCAGTT CACCTGTGGT GTGCTCTGCC GCTGGAGTGA GGAGTGTGGC 
CGGGCTGCAG GCAGGGCTTG TGGCTTTGCA CAGCCAGGGT GTAGTTGTCC TGGGGAGGCA 
GGGGCCAACC CCGCCACCAC CACATCTCCG GGGCCTCCGG TTGCCCACAC CCTGTCCAAT 
GCCCTGGTGC CCGGGGGCCC AGCCCCTCCT GCTGAGGCCG ACTTGCACTC GGGGAGCAGC 
AATGACCTGT TCACCACCGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG 
AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTAT ACATGGCGCA GACAGTGCAC 
GGCGTGTGGG AGGAGTGGGG GTCCTGGAGC CTGTGCTCCC GCAGCTGCGG GCGGGGGTCT 
CGGAGCCGGA TGCGGACCTG CGTGCCCCCC CAGCACGGCG GCAAGGCCTG CGAGGGTCCC 
GAGCTGCAGA CTAAACTCTG CAGTATGGCC GCCTGCCCGG TGGAAGGCCA GTGGCTAGAA 
TGGGGTCCCT GGGGCCCATG CTCATCATCT TGTGCCAATG GGACCCAGCA GCGCAGCCGG 
AAATGCAGTG TGGCGGGTCC AGCCTGGGCC ACGTGCGCAG GTGCCCTCAC GGATACCCGT 
GAGTGCAGCA ATCTCGATTG CCCGGCCACT GACGGCAAGT GGGGGCCGTG GAACGCGTGG 
AGCCTGTGCT CCAAGACGTG TGACACGGGC TGGCAACGCC GCTTCCGCAT GTGCCAGGCT 
TCTGGCACAC AGGGCTACCC TTGCGAGGGC ACAGGAGAGG AGGTGAAACC CTGCAGTGAG 
AAGAGGTGTC CAGCCTTCCA TGAGATGTGC AGGGATGAGT ACGTGATGTT GATGACATGG 
AAGAGGGCGG CAGCTGGCGA GATCATTTAC AACAAGTGTC CCCCTAATGC CTCGGGTTCT 
GCTAGCCGCC GCTGTCTCCT CAGTGCCCAG GGCGTAGCAT ACTGGGGACT GCCCAGCTTT 
GCTCGTTGCA TATCCCATGA ATACCGCTAC CTGTACCTGT CACTTCGGGA ACACCTGGCT 
AAGGGCCAGC GCATGCTGGC AGGTGAGGGC ATGTCACAGG TGGTGCGGAG CCTGCAGGAG 
CTACTGGCAC GGCGCACTTA CTACAGCGGG GACCTGCTCT TCTCTGTGGA CATCCTAAGG 
AACGTCACTG ACACCTTCAA GAGGGCCACC TATGTCCCTT CCGCCGATGA CGTGCAGCGT 
TTCTTCCAGG TGGTGAGCTT CATGGTGGAT TCAGAAAACA AGGACAAATG GGATGATGCT 
CAGCAGGTGT CACCGGGCTC TGTGCACCTG CTGCGTGTTG TGGAAGATTT CATTCACCTC 
GTGGGCGACG CTCTCAAGGC CTTCCAGAGC TCTCTCATTG TCACGGACAA TCTGGTGATC 
AGCATTCAGA GAGAGCCTAT CTCCGCCGTG TCCAGTGACA TCACGTTTCC CATGCGGGGC 
CGCAGGGGCA TGAAGGACTG GGTGCGACAC TCAGAGGATC GTCTCTTTCT ACCCAAGGAG 
GTGCTCAGCC TGTCCTCCCC AGGAAAGCCA GCCACACCTG GGGCAGCCAC AGCAGGCAGC 
CCGGGCAGGG GGAGGGGCCC AGGAACGGTG CCCCCTGGCC CAGGCCACGC CCACCAGCGC 
CTTCTCCCAG CTGACCCCGA AGAGTCCTCC TCCTACTTTG TGATCGGTGC TGTGCTCTAC 
CGCACCCTTG GCCTCATCCT GCCGCCCCCC AGGCCTCCAC TTGCTGTCAC CTCCCGGGTG 
ATGACAGTGA CTGTGCGTCC CCCCACCCAG CCTCCAGCTG AGCCCCTCAT TACAGTGGAA 
CTCTCGTACA TCATCAATGG CACCACCGAT CCCCACTGTG CCAGCTGGGA CTACTCCAGA 
GCAGATACCA ACTCGGGGGA CTGGAACACT GAGAGCTGCC AGACCTTGGA GACCCAGGCG 
GCTCACACCC GCTGCCAGTG CCAGCACCTG TCCACCTTTG CCGTCCTGGC CCAGCCACCC 
AAGGACCTGA CCCTGGAGCT GGCAGGTGCT CCCTCTGTCC CCCTGGTGAT CGGCTGTGCA 
GTGTCCTGCA TGGCTCTGCT CACCCTGCTG GCCATCTATG CAGCCTTCTG GAGGTTTATA 
AAATCAGAAC GCTCCATCAT CTTGCTGAAC TTCTGCCTGT CCATCCTGGC TTCCAACATT 
CTGATCCTGG TGGGCCAGTC CCGGGTGCTG AGCAAGGGCG TATGCACCAT GACGGCTGCC 
TTCCTACACT TCTTCTTTCT GTCCTCCTTT TGCTGGGTGC TTACAGAGGC CTGGCAATCC 
TATCTGGCTG TCATCGGGCG GATGCGCACC CGCCTGGTTC GCAAGCGCTT CCTCTGCCTG 
GGCTGGGGTC TACCTGCCCT GGTGGTGGCT GTGTCTGTTG GCTTTACTCG CACCAAAGGA 
TATGGTACAT CCAGCTACTG CTGGCTGTCC CTAGAGGGCG GCCTGCTCTA TGCCTTCGTG 
GGTCCAGCAG CAGTCATTGT CCTGGTGAAC ATGCTCATCG GGATTATCGT CTTCAACAAG 
CTCATGGCTC GCGATGGCGT CTCAGACAAA TCTAAGAAGC AGAGGGCTGG GTCGGAGCGG 
TGCCCCTGGG CCAGCCTGCT CCTTCCCTGC TCAGCGTGTG GAGCGGTCCC CAGCCCCCTG 
CTCAGCTCAG CCTCGGCCAG GAATGCCATC CGACTGTCTT GGAATCTCTG GGGATATGGC 
TCTCAACTGT GTCTTTTTCC AAAGCTTCCA AGGGCTTCAC TCTGGAGTTC CTGCGTGGTA 
CTGCCTCTCC TGGCGCTTAC CTGGATGTCT GCCGTCCTGG CCATGACAGA TCGCCGCTCC 
GTCCTCTTCC AGGCACTCTT TGCCGTTTTC AACTCTGCAC AAGGCTTTGT CATCACCGCT 
GTGCACTGCT TCCTGCGCCG AGAGGTCCAG GATGTGGTAA AGTGTCAGAT GGGTGTGTGT 
CGGGCTGATG AGAGTGAAGA CTCCCCAGAC TCGTGCAAGA ACGGGCAGCT GCAGATCCTG 
TCAGACTTTG AAAAGGACGT GGATCTGGCT TGTCAGACAG TTCTGTTCAA GGAGGTCAAC 
ACCTGCAACC CGTCCACCAT TACCGGCACT CTGTCCCGCC TGTCTCTGGA TGAGGATGAG 
GAGCCCAAGT CCTGTCTCGT GGGTCCTGAG GGTGGCCTCA GCTTCTCACC ACTGCCTGGG 
AACATCCTGG TACCCATGGC AGCCTCACCA GGTCTAGGGG AGCCACCACC ACCCCAGGAG 
ACCAACCCTG TGTACATGTG TGGGGAGGGT GGCCTGCGGC AGTTGGACCT TACATGGATA 
CGGCAGAGTG AACCAGGCTC TGAGGGGGAC TACATGGTTC TGCCCCGGCG GACTTTGAGC 
CTGCAGCCTG GTGGTGGGGG TACAGCGGGT GAGGAGGCCC CAAGGGCCCG GCCTGAGGGG 
ACCCCCCGGC GGGCTGCCAA AACGGTAGCC CACACTGAAG GCTACCCCAG CTTCCTGTCT 
GTGGAGCACT CGGGTCTAGG GCTGGGCCCT GCCTATGGGT CTCTCCAGAA CCCGTATGGA 
ATGACCTTCC AACCACCACC ACCAACACCC AGCGCCCGCC AAGTACCAGA GCCAGGAGAA 
CGTAGCCGGA CCATGCCCCG TACAGTGCCT GGTTCCACCA TGAAGCTGGG CTCCCTTGAG 
CGAAAGAAGC TTCGGTATTC GGACTTGGAC TTTGAGAAGG TGATGCACAC TCGGAAACGG 
CACTCGGAAC TCTACCACGA ACTCAACCAG AAGTTCCACA CTTTCGACCG CTACCGTAGC 
CAGTCCTCAG CCAAGGAGAA ACCCAGCCCC CCCGGGGGAC GCCCTGGCTT GTCCCAGCAC 
AGGAGGCATC AAAGCTGGAG CACCTTCAAA TCTATGACAC TGGGCTCACT GCCCCCCAAG 
CCCCGAGAAC GGCTGGCCCT GCACCGGACA GCAGCCTGGG AGCCCACAGA ACCGCCAGAC 
GGCGACTTCC AGACAGAGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adgrb2 ( XM_011240512.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011238814.1 Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant X5, mRNA.

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CloneID	OMu73519
Clone ID Related Accession (Same CDS sequence)	XM_011240512.1
Accession Version	XM_011240512.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4584bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	adhesion G protein-coupled receptor B2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCAG CCTGTCCCCT CTTACTGTCT GTGATTCTGT CCCTGCGCCT GGCCACGGCC 
TTCGACCCTG CCCCCAGTGC CTGCTCTGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTC 
TCGCTGCAGG ACCTCTTTCC CACCATCGCC TCGGGCTGCT CCTGGACCCT GGAGAACCCA 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGGC AGGAGCAGGT TTGCACACAC 
TTTGCCCCGC GCCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTTACCTG CCTGCGGCCT 
GGTCCAGAGG AAGCCACAGC CCGGGCTGAG TCGGAGGTGG GACGGCCAGA GGAGGAGGAG 
GAGGAGGCGG CGGCAGCAGC ATCAGGGTTG GAGTTGTGTG GTGGCTCAGG CCCCTTTACC 
TTTCTGCACT TCGACAAGAA CTTTGTGCAG CTGTGCCTGT CGGCTGAGCC CTCTGAGGCC 
CCTCGTCTGC TAGCGCCTGC TGCCCTGGCC TTCCGTTTTG TGGAGGTCTT GCTGATCAAC 
AACAACAACT CCAGCCAGTT CACCTGTGGT GTGCTCTGCC GCTGGAGTGA GGAGTGTGGC 
CGGGCTGCAG GCAGGGCTTG TGGCTTTGCA CAGCCAGGGT GTAGTTGTCC TGGGGAGGCA 
GGGGCCAACC CCGCCACCAC CACATCTCCG GGGCCTCCGG TTGCCCACAC CCTGTCCAAT 
GCCCTGGTGC CCGGGGGCCC AGCCCCTCCT GCTGAGGCCG ACTTGCACTC GGGGAGCAGC 
AATGACCTGT TCACCACCGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG 
AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTAT ACATGGCGCA GACAGGCGAC 
CCAGCAGCTG AGGAGTGGTC CCCGTGGAGC GTGTGTTCCC TGACGTGTGG GCAGGGTCTG 
CAGGTGCGGA CCCGCTCCTG CGTGTCCTCC CCCTATGGGA CCCTGTGCAG CGGGCCCCTT 
CGGGAGACCC GGCCTTGCAA CAATTCAGCC ACCTGCCCAG TGCACGGCGT GTGGGAGGAG 
TGGGGGTCCT GGAGCCTGTG CTCCCGCAGC TGCGGGCGGG GGTCTCGGAG CCGGATGCGG 
ACCTGCGTGC CCCCCCAGCA CGGCGGCAAG GCCTGCGAGG GTCCCGAGCT GCAGACTAAA 
CTCTGCAGTA TGGCCGCCTG CCCGGTGGAA GGCCAGTGGC TAGAATGGGG TCCCTGGGGC 
CCATGCTCAT CATCTTGTGC CAATGGGACC CAGCAGCGCA GCCGGAAATG CAGTGTGGCG 
GGTCCAGCCT GGGCCACGTG CGCAGGTGCC CTCACGGATA CCCGTGAGTG CAGCAATCTC 
GATTGCCCGG CCACTGACGG CAAGTGGGGG CCGTGGAACG CGTGGAGCCT GTGCTCCAAG 
ACGTGTGACA CGGGCTGGCA ACGCCGCTTC CGCATGTGCC AGGCTTCTGG CACACAGGGC 
TACCCTTGCG AGGGCACAGG AGAGGAGGTG AAACCCTGCA GTGAGAAGAG GTGTCCAGCC 
TTCCATGAGA TGTGCAGGGA TGAGTACGTG ATGTTGATGA CATGGAAGAG GGCGGCAGCT 
GGCGAGATCA TTTACAACAA GTGTCCCCCT AATGCCTCGG GTTCTGCTAG CCGCCGCTGT 
CTCCTCAGTG CCCAGGGCGT AGCATACTGG GGACTGCCCA GCTTTGCTCG TTGCATATCC 
CATGAATACC GCTACCTGTA CCTGTCACTT CGGGAACACC TGGCTAAGGG CCAGCGCATG 
CTGGCAGGTG AGGGCATGTC ACAGGTGGTG CGGAGCCTGC AGGAGCTACT GGCACGGCGC 
ACTTACTACA GCGGGGACCT GCTCTTCTCT GTGGACATCC TAAGGAACGT CACTGACACC 
TTCAAGAGGG CCACCTATGT CCCTTCCGCC GATGACGTGC AGCGTTTCTT CCAGGTGGTG 
AGCTTCATGG TGGATTCAGA AAACAAGGAC AAATGGGATG ATGCTCAGCA GGTGTCACCG 
GGCTCTGTGC ACCTGCTGCG TGTTGTGGAA GATTTCATTC ACCTCGTGGG CGACGCTCTC 
AAGGCCTTCC AGAGCTCTCT CATTGTCACG GACAATCTGG TGATCAGCAT TCAGAGAGAG 
CCTATCTCCG CCGTGTCCAG TGACATCACG TTTCCCATGC GGGGCCGCAG GGGCATGAAG 
GACTGGGTGC GACACTCAGA GGATCGTCTC TTTCTACCCA AGGAGGTGCT CAGCCTGTCC 
TCCCCAGGAA AGCCAGCCAC ACCTGGGGCA GCCACAGCAG GCAGCCCGGG CAGGGGGAGG 
GGCCCAGGAA CGGTGCCCCC TGGCCCAGGC CACGCCCACC AGCGCCTTCT CCCAGCTGAC 
CCCGAAGAGT CCTCCTCCTA CTTTGTGATC GGTGCTGTGC TCTACCGCAC CCTTGGCCTC 
ATCCTGCCGC CCCCCAGGCC TCCACTTGCT GTCACCTCCC GGGTGATGAC AGTGACTGTG 
CGTCCCCCCA CCCAGCCTCC AGCTGAGCCC CTCATTACAG TGGAACTCTC GTACATCATC 
AATGGCACCA CCGATCCCCA CTGTGCCAGC TGGGACTACT CCAGAGCAGA TACCAACTCG 
GGGGACTGGA ACACTGAGAG CTGCCAGACC TTGGAGACCC AGGCGGCTCA CACCCGCTGC 
CAGTGCCAGC ACCTGTCCAC CTTTGCCGTC CTGGCCCAGC CACCCAAGGA CCTGACCCTG 
GAGCTGGCAG GTGCTCCCTC TGTCCCCCTG GTGATCGGCT GTGCAGTGTC CTGCATGGCT 
CTGCTCACCC TGCTGGCCAT CTATGCAGCC TTCTGGAGGT TTATAAAATC AGAACGCTCC 
ATCATCTTGC TGAACTTCTG CCTGTCCATC CTGGCTTCCA ACATTCTGAT CCTGGTGGGC 
CAGTCCCGGG TGCTGAGCAA GGGCGTATGC ACCATGACGG CTGCCTTCCT ACACTTCTTC 
TTTCTGTCCT CCTTTTGCTG GGTGCTTACA GAGGCCTGGC AATCCTATCT GGCTGTCATC 
GGGCGGATGC GCACCCGCCT GGTTCGCAAG CGCTTCCTCT GCCTGGGCTG GGGTCTACCT 
GCCCTGGTGG TGGCTGTGTC TGTTGGCTTT ACTCGCACCA AAGGATATGG TACATCCAGC 
TACTGCTGGC TGTCCCTAGA GGGCGGCCTG CTCTATGCCT TCGTGGGTCC AGCAGCAGTC 
ATTGTCCTGG TGAACATGCT CATCGGGATT ATCGTCTTCA ACAAGCTCAT GGCTCGCGAT 
GGCGTCTCAG ACAAATCTAA GAAGCAGAGG GCTGGGGCTT CACTCTGGAG TTCCTGCGTG 
GTACTGCCTC TCCTGGCGCT TACCTGGATG TCTGCCGTCC TGGCCATGAC AGATCGCCGC 
TCCGTCCTCT TCCAGGCACT CTTTGCCGTT TTCAACTCTG CACAAGGCTT TGTCATCACC 
GCTGTGCACT GCTTCCTGCG CCGAGAGGTC CAGGATGTGG TAAAGTGTCA GATGGGTGTG 
TGTCGGGCTG ATGAGAGTGA AGACTCCCCA GACTCGTGCA AGAACGGGCA GCTGCAGATC 
CTGTCAGACT TTGAAAAGGA CGTGGATCTG GCTTGTCAGA CAGTTCTGTT CAAGGAGGTC 
AACACCTGCA ACCCGTCCAC CATTACCGGC ACTCTGTCCC GCCTGTCTCT GGATGAGGAT 
GAGGAGCCCA AGTCCTGTCT CGTGGGTCCT GAGGGTGGCC TCAGCTTCTC ACCACTGCCT 
GGGAACATCC TGGTACCCAT GGCAGCCTCA CCAGGTCTAG GGGAGCCACC ACCACCCCAG 
GAGACCAACC CTGTGTACAT GTGTGGGGAG GGTGGCCTGC GGCAGTTGGA CCTTACATGG 
ATACGGCAGA GTGAACCAGG CTCTGAGGGG GACTACATGG TTCTGCCCCG GCGGACTTTG 
AGCCTGCAGC CTGGTGGTGG GGGTACAGCG GGTGAGGAGG CCCCAAGGGC CCGGCCTGAG 
GGGACCCCCC GGCGGGCTGC CAAAACGGTA GCCCACACTG AAGGCTACCC CAGCTTCCTG 
TCTGTGGAGC ACTCGGGTCT AGGGCTGGGC CCTGCCTATG GGTCTCTCCA GAACCCGTAT 
GGAATGACCT TCCAACCACC ACCACCAACA CCCAGCGCCC GCCAAGTACC AGAGCCAGGA 
GAACGTAGCC GGACCATGCC CCGTACAGTG CCTGGTTCCA CCATGAAGCT GGGCTCCCTT 
GAGCGAAAGA AGCTTCGGTA TTCGGACTTG GACTTTGAGG TGATGCACAC TCGGAAACGG 
CACTCGGAAC TCTACCACGA ACTCAACCAG AAGTTCCACA CTTTCGACCG CTACCGTAGC 
CAGTCCTCAG CCAAGGAGAA ACCCAGCCCC CCCGGGGGAC GCCCTGGCTT GTCCCAGCAC 
AGGAGGCATC AAAGCTGGAG CACCTTCAAA TCTATGACAC TGGGCTCACT GCCCCCCAAG 
CCCCGAGAAC GGCTGGCCCT GCACCGGACA GCAGCCTGGG AGCCCACAGA ACCGCCAGAC 
GGCGACTTCC AGACAGAGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011238814.1
CDS	16..4599
Translation	MTPACPLLLSVILSLRLATAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCTHFAPRLLPLDHYLVNFTCLRPGPEEATARAESEVGRPEEEEEEAAAAASGLELCGGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGANPATTTSPGPPVAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSATCPVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSSSCANGTQQRSRKCSVAGPAWATCAGALTDTRECSNLDCPATDGKWGPWNAWSLCSKTCDTGWQRRFRMCQASGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKRAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDSENKDKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPISAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSSPGKPATPGAATAGSPGRGRGPGTVPPGPGHAHQRLLPADPEESSSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADTNSGDWNTESCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGAPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNILILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGVSDKSKKQRAGASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGGLSFSPLPGNILVPMAASPGLGEPPPPQETNPVYMCGEGGLRQLDLTWIRQSEPGSEGDYMVLPRRTLSLQPGGGGTAGEEAPRARPEGTPRRAAKTVAHTEGYPSFLSVEHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVPEPGERSRTMPRTVPGSTMKLGSLERKKLRYSDLDFEVMHTRKRHSELYHELNQKFHTFDRYRSQSSAKEKPSPPGGRPGLSQHRRHQSWSTFKSMTLGSLPPKPRERLALHRTAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_011240512.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240512.1

ORF Insert Sequence:

ATGACCCCAG CCTGTCCCCT CTTACTGTCT GTGATTCTGT CCCTGCGCCT GGCCACGGCC 
TTCGACCCTG CCCCCAGTGC CTGCTCTGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTC 
TCGCTGCAGG ACCTCTTTCC CACCATCGCC TCGGGCTGCT CCTGGACCCT GGAGAACCCA 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGGC AGGAGCAGGT TTGCACACAC 
TTTGCCCCGC GCCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTTACCTG CCTGCGGCCT 
GGTCCAGAGG AAGCCACAGC CCGGGCTGAG TCGGAGGTGG GACGGCCAGA GGAGGAGGAG 
GAGGAGGCGG CGGCAGCAGC ATCAGGGTTG GAGTTGTGTG GTGGCTCAGG CCCCTTTACC 
TTTCTGCACT TCGACAAGAA CTTTGTGCAG CTGTGCCTGT CGGCTGAGCC CTCTGAGGCC 
CCTCGTCTGC TAGCGCCTGC TGCCCTGGCC TTCCGTTTTG TGGAGGTCTT GCTGATCAAC 
AACAACAACT CCAGCCAGTT CACCTGTGGT GTGCTCTGCC GCTGGAGTGA GGAGTGTGGC 
CGGGCTGCAG GCAGGGCTTG TGGCTTTGCA CAGCCAGGGT GTAGTTGTCC TGGGGAGGCA 
GGGGCCAACC CCGCCACCAC CACATCTCCG GGGCCTCCGG TTGCCCACAC CCTGTCCAAT 
GCCCTGGTGC CCGGGGGCCC AGCCCCTCCT GCTGAGGCCG ACTTGCACTC GGGGAGCAGC 
AATGACCTGT TCACCACCGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG 
AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTAT ACATGGCGCA GACAGGCGAC 
CCAGCAGCTG AGGAGTGGTC CCCGTGGAGC GTGTGTTCCC TGACGTGTGG GCAGGGTCTG 
CAGGTGCGGA CCCGCTCCTG CGTGTCCTCC CCCTATGGGA CCCTGTGCAG CGGGCCCCTT 
CGGGAGACCC GGCCTTGCAA CAATTCAGCC ACCTGCCCAG TGCACGGCGT GTGGGAGGAG 
TGGGGGTCCT GGAGCCTGTG CTCCCGCAGC TGCGGGCGGG GGTCTCGGAG CCGGATGCGG 
ACCTGCGTGC CCCCCCAGCA CGGCGGCAAG GCCTGCGAGG GTCCCGAGCT GCAGACTAAA 
CTCTGCAGTA TGGCCGCCTG CCCGGTGGAA GGCCAGTGGC TAGAATGGGG TCCCTGGGGC 
CCATGCTCAT CATCTTGTGC CAATGGGACC CAGCAGCGCA GCCGGAAATG CAGTGTGGCG 
GGTCCAGCCT GGGCCACGTG CGCAGGTGCC CTCACGGATA CCCGTGAGTG CAGCAATCTC 
GATTGCCCGG CCACTGACGG CAAGTGGGGG CCGTGGAACG CGTGGAGCCT GTGCTCCAAG 
ACGTGTGACA CGGGCTGGCA ACGCCGCTTC CGCATGTGCC AGGCTTCTGG CACACAGGGC 
TACCCTTGCG AGGGCACAGG AGAGGAGGTG AAACCCTGCA GTGAGAAGAG GTGTCCAGCC 
TTCCATGAGA TGTGCAGGGA TGAGTACGTG ATGTTGATGA CATGGAAGAG GGCGGCAGCT 
GGCGAGATCA TTTACAACAA GTGTCCCCCT AATGCCTCGG GTTCTGCTAG CCGCCGCTGT 
CTCCTCAGTG CCCAGGGCGT AGCATACTGG GGACTGCCCA GCTTTGCTCG TTGCATATCC 
CATGAATACC GCTACCTGTA CCTGTCACTT CGGGAACACC TGGCTAAGGG CCAGCGCATG 
CTGGCAGGTG AGGGCATGTC ACAGGTGGTG CGGAGCCTGC AGGAGCTACT GGCACGGCGC 
ACTTACTACA GCGGGGACCT GCTCTTCTCT GTGGACATCC TAAGGAACGT CACTGACACC 
TTCAAGAGGG CCACCTATGT CCCTTCCGCC GATGACGTGC AGCGTTTCTT CCAGGTGGTG 
AGCTTCATGG TGGATTCAGA AAACAAGGAC AAATGGGATG ATGCTCAGCA GGTGTCACCG 
GGCTCTGTGC ACCTGCTGCG TGTTGTGGAA GATTTCATTC ACCTCGTGGG CGACGCTCTC 
AAGGCCTTCC AGAGCTCTCT CATTGTCACG GACAATCTGG TGATCAGCAT TCAGAGAGAG 
CCTATCTCCG CCGTGTCCAG TGACATCACG TTTCCCATGC GGGGCCGCAG GGGCATGAAG 
GACTGGGTGC GACACTCAGA GGATCGTCTC TTTCTACCCA AGGAGGTGCT CAGCCTGTCC 
TCCCCAGGAA AGCCAGCCAC ACCTGGGGCA GCCACAGCAG GCAGCCCGGG CAGGGGGAGG 
GGCCCAGGAA CGGTGCCCCC TGGCCCAGGC CACGCCCACC AGCGCCTTCT CCCAGCTGAC 
CCCGAAGAGT CCTCCTCCTA CTTTGTGATC GGTGCTGTGC TCTACCGCAC CCTTGGCCTC 
ATCCTGCCGC CCCCCAGGCC TCCACTTGCT GTCACCTCCC GGGTGATGAC AGTGACTGTG 
CGTCCCCCCA CCCAGCCTCC AGCTGAGCCC CTCATTACAG TGGAACTCTC GTACATCATC 
AATGGCACCA CCGATCCCCA CTGTGCCAGC TGGGACTACT CCAGAGCAGA TACCAACTCG 
GGGGACTGGA ACACTGAGAG CTGCCAGACC TTGGAGACCC AGGCGGCTCA CACCCGCTGC 
CAGTGCCAGC ACCTGTCCAC CTTTGCCGTC CTGGCCCAGC CACCCAAGGA CCTGACCCTG 
GAGCTGGCAG GTGCTCCCTC TGTCCCCCTG GTGATCGGCT GTGCAGTGTC CTGCATGGCT 
CTGCTCACCC TGCTGGCCAT CTATGCAGCC TTCTGGAGGT TTATAAAATC AGAACGCTCC 
ATCATCTTGC TGAACTTCTG CCTGTCCATC CTGGCTTCCA ACATTCTGAT CCTGGTGGGC 
CAGTCCCGGG TGCTGAGCAA GGGCGTATGC ACCATGACGG CTGCCTTCCT ACACTTCTTC 
TTTCTGTCCT CCTTTTGCTG GGTGCTTACA GAGGCCTGGC AATCCTATCT GGCTGTCATC 
GGGCGGATGC GCACCCGCCT GGTTCGCAAG CGCTTCCTCT GCCTGGGCTG GGGTCTACCT 
GCCCTGGTGG TGGCTGTGTC TGTTGGCTTT ACTCGCACCA AAGGATATGG TACATCCAGC 
TACTGCTGGC TGTCCCTAGA GGGCGGCCTG CTCTATGCCT TCGTGGGTCC AGCAGCAGTC 
ATTGTCCTGG TGAACATGCT CATCGGGATT ATCGTCTTCA ACAAGCTCAT GGCTCGCGAT 
GGCGTCTCAG ACAAATCTAA GAAGCAGAGG GCTGGGGCTT CACTCTGGAG TTCCTGCGTG 
GTACTGCCTC TCCTGGCGCT TACCTGGATG TCTGCCGTCC TGGCCATGAC AGATCGCCGC 
TCCGTCCTCT TCCAGGCACT CTTTGCCGTT TTCAACTCTG CACAAGGCTT TGTCATCACC 
GCTGTGCACT GCTTCCTGCG CCGAGAGGTC CAGGATGTGG TAAAGTGTCA GATGGGTGTG 
TGTCGGGCTG ATGAGAGTGA AGACTCCCCA GACTCGTGCA AGAACGGGCA GCTGCAGATC 
CTGTCAGACT TTGAAAAGGA CGTGGATCTG GCTTGTCAGA CAGTTCTGTT CAAGGAGGTC 
AACACCTGCA ACCCGTCCAC CATTACCGGC ACTCTGTCCC GCCTGTCTCT GGATGAGGAT 
GAGGAGCCCA AGTCCTGTCT CGTGGGTCCT GAGGGTGGCC TCAGCTTCTC ACCACTGCCT 
GGGAACATCC TGGTACCCAT GGCAGCCTCA CCAGGTCTAG GGGAGCCACC ACCACCCCAG 
GAGACCAACC CTGTGTACAT GTGTGGGGAG GGTGGCCTGC GGCAGTTGGA CCTTACATGG 
ATACGGCAGA GTGAACCAGG CTCTGAGGGG GACTACATGG TTCTGCCCCG GCGGACTTTG 
AGCCTGCAGC CTGGTGGTGG GGGTACAGCG GGTGAGGAGG CCCCAAGGGC CCGGCCTGAG 
GGGACCCCCC GGCGGGCTGC CAAAACGGTA GCCCACACTG AAGGCTACCC CAGCTTCCTG 
TCTGTGGAGC ACTCGGGTCT AGGGCTGGGC CCTGCCTATG GGTCTCTCCA GAACCCGTAT 
GGAATGACCT TCCAACCACC ACCACCAACA CCCAGCGCCC GCCAAGTACC AGAGCCAGGA 
GAACGTAGCC GGACCATGCC CCGTACAGTG CCTGGTTCCA CCATGAAGCT GGGCTCCCTT 
GAGCGAAAGA AGCTTCGGTA TTCGGACTTG GACTTTGAGG TGATGCACAC TCGGAAACGG 
CACTCGGAAC TCTACCACGA ACTCAACCAG AAGTTCCACA CTTTCGACCG CTACCGTAGC 
CAGTCCTCAG CCAAGGAGAA ACCCAGCCCC CCCGGGGGAC GCCCTGGCTT GTCCCAGCAC 
AGGAGGCATC AAAGCTGGAG CACCTTCAAA TCTATGACAC TGGGCTCACT GCCCCCCAAG 
CCCCGAGAAC GGCTGGCCCT GCACCGGACA GCAGCCTGGG AGCCCACAGA ACCGCCAGAC 
GGCGACTTCC AGACAGAGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adgrb2 ( XM_011240513.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011238815.1 Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant X8, mRNA.

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CloneID	OMu73520
Clone ID Related Accession (Same CDS sequence)	NM_001377057.1 , XM_011240513.1
Accession Version	XM_011240513.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	adhesion G protein-coupled receptor B2 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCAG CCTGTCCCCT CTTACTGTCT GTGATTCTGT CCCTGCGCCT GGCCACGGCC 
TTCGACCCTG CCCCCAGTGC CTGCTCTGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTC 
TCGCTGCAGG ACCTCTTTCC CACCATCGCC TCGGGCTGCT CCTGGACCCT GGAGAACCCA 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGGC AGGAGCAGGT TTGCACACAC 
TTTGCCCCGC GCCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTTACCTG CCTGCGGCCT 
GGTCCAGAGG AAGCCACAGC CCGGGCTGAG TCGGAGGTGG GACGGCCAGA GGAGGAGGAG 
GAGGAGGCGG CGGCAGCAGC ATCAGGGTTG GAGTTGTGTG GTGGCTCAGG CCCCTTTACC 
TTTCTGCACT TCGACAAGAA CTTTGTGCAG CTGTGCCTGT CGGCTGAGCC CTCTGAGGCC 
CCTCGTCTGC TAGCGCCTGC TGCCCTGGCC TTCCGTTTTG TGGAGGTCTT GCTGATCAAC 
AACAACAACT CCAGCCAGTT CACCTGTGGT GTGCTCTGCC GCTGGAGTGA GGAGTGTGGC 
CGGGCTGCAG GCAGGGCTTG TGGCTTTGCA CAGCCAGGGT GTAGTTGTCC TGGGGAGGCA 
GGGGCCAACC CCGCCACCAC CACATCTCCG GGGCCTCCGG TTGCCCACAC CCTGTCCAAT 
GCCCTGGTGC CCGGGGGCCC AGCCCCTCCT GCTGAGGCCG ACTTGCACTC GGGGAGCAGC 
AATGACCTGT TCACCACCGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG 
AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTAT ACATGGCGCA GACAGTGCAC 
GGCGTGTGGG AGGAGTGGGG GTCCTGGAGC CTGTGCTCCC GCAGCTGCGG GCGGGGGTCT 
CGGAGCCGGA TGCGGACCTG CGTGCCCCCC CAGCACGGCG GCAAGGCCTG CGAGGGTCCC 
GAGCTGCAGA CTAAACTCTG CAGTATGGCC GCCTGCCCGG TGGAAGGCCA GTGGCTAGAA 
TGGGGTCCCT GGGGCCCATG CTCATCATCT TGTGCCAATG GGACCCAGCA GCGCAGCCGG 
AAATGCAGTG TGGCGGGTCC AGCCTGGGCC ACGTGCGCAG GTGCCCTCAC GGATACCCGT 
GAGTGCAGCA ATCTCGATTG CCCGGCCACT GACGGCAAGT GGGGGCCGTG GAACGCGTGG 
AGCCTGTGCT CCAAGACGTG TGACACGGGC TGGCAACGCC GCTTCCGCAT GTGCCAGGCT 
TCTGGCACAC AGGGCTACCC TTGCGAGGGC ACAGGAGAGG AGGTGAAACC CTGCAGTGAG 
AAGAGGTGTC CAGCCTTCCA TGAGATGTGC AGGGATGAGT ACGTGATGTT GATGACATGG 
AAGAGGGCGG CAGCTGGCGA GATCATTTAC AACAAGTGTC CCCCTAATGC CTCGGGTTCT 
GCTAGCCGCC GCTGTCTCCT CAGTGCCCAG GGCGTAGCAT ACTGGGGACT GCCCAGCTTT 
GCTCGTTGCA TATCCCATGA ATACCGCTAC CTGTACCTGT CACTTCGGGA ACACCTGGCT 
AAGGGCCAGC GCATGCTGGC AGGTGAGGGC ATGTCACAGG TGGTGCGGAG CCTGCAGGAG 
CTACTGGCAC GGCGCACTTA CTACAGCGGG GACCTGCTCT TCTCTGTGGA CATCCTAAGG 
AACGTCACTG ACACCTTCAA GAGGGCCACC TATGTCCCTT CCGCCGATGA CGTGCAGCGT 
TTCTTCCAGG TGGTGAGCTT CATGGTGGAT TCAGAAAACA AGGACAAATG GGATGATGCT 
CAGCAGGTGT CACCGGGCTC TGTGCACCTG CTGCGTGTTG TGGAAGATTT CATTCACCTC 
GTGGGCGACG CTCTCAAGGC CTTCCAGAGC TCTCTCATTG TCACGGACAA TCTGGTGATC 
AGCATTCAGA GAGAGCCTAT CTCCGCCGTG TCCAGTGACA TCACGTTTCC CATGCGGGGC 
CGCAGGGGCA TGAAGGACTG GGTGCGACAC TCAGAGGATC GTCTCTTTCT ACCCAAGGAG 
GTGCTCAGCC TGTCCTCCCC AGGAAAGCCA GCCACACCTG GGGCAGCCAC AGCAGGCAGC 
CCGGGCAGGG GGAGGGGCCC AGGAACGGTG CCCCCTGGCC CAGGCCACGC CCACCAGCGC 
CTTCTCCCAG CTGACCCCGA AGAGTCCTCC TCCTACTTTG TGATCGGTGC TGTGCTCTAC 
CGCACCCTTG GCCTCATCCT GCCGCCCCCC AGGCCTCCAC TTGCTGTCAC CTCCCGGGTG 
ATGACAGTGA CTGTGCGTCC CCCCACCCAG CCTCCAGCTG AGCCCCTCAT TACAGTGGAA 
CTCTCGTACA TCATCAATGG CACCACCGAT CCCCACTGTG CCAGCTGGGA CTACTCCAGA 
GCAGATACCA ACTCGGGGGA CTGGAACACT GAGAGCTGCC AGACCTTGGA GACCCAGGCG 
GCTCACACCC GCTGCCAGTG CCAGCACCTG TCCACCTTTG CCGTCCTGGC CCAGCCACCC 
AAGGACCTGA CCCTGGAGCT GGCAGGTGCT CCCTCTGTCC CCCTGGTGAT CGGCTGTGCA 
GTGTCCTGCA TGGCTCTGCT CACCCTGCTG GCCATCTATG CAGCCTTCTG GAGGTTTATA 
AAATCAGAAC GCTCCATCAT CTTGCTGAAC TTCTGCCTGT CCATCCTGGC TTCCAACATT 
CTGATCCTGG TGGGCCAGTC CCGGGTGCTG AGCAAGGGCG TATGCACCAT GACGGCTGCC 
TTCCTACACT TCTTCTTTCT GTCCTCCTTT TGCTGGGTGC TTACAGAGGC CTGGCAATCC 
TATCTGGCTG TCATCGGGCG GATGCGCACC CGCCTGGTTC GCAAGCGCTT CCTCTGCCTG 
GGCTGGGGTC TACCTGCCCT GGTGGTGGCT GTGTCTGTTG GCTTTACTCG CACCAAAGGA 
TATGGTACAT CCAGCTACTG CTGGCTGTCC CTAGAGGGCG GCCTGCTCTA TGCCTTCGTG 
GGTCCAGCAG CAGTCATTGT CCTGGTGAAC ATGCTCATCG GGATTATCGT CTTCAACAAG 
CTCATGGCTC GCGATGGCGT CTCAGACAAA TCTAAGAAGC AGAGGGCTGG GGCTTCACTC 
TGGAGTTCCT GCGTGGTACT GCCTCTCCTG GCGCTTACCT GGATGTCTGC CGTCCTGGCC 
ATGACAGATC GCCGCTCCGT CCTCTTCCAG GCACTCTTTG CCGTTTTCAA CTCTGCACAA 
GGCTTTGTCA TCACCGCTGT GCACTGCTTC CTGCGCCGAG AGGTCCAGGA TGTGGTAAAG 
TGTCAGATGG GTGTGTGTCG GGCTGATGAG AGTGAAGACT CCCCAGACTC GTGCAAGAAC 
GGGCAGCTGC AGATCCTGTC AGACTTTGAA AAGGACGTGG ATCTGGCTTG TCAGACAGTT 
CTGTTCAAGG AGGTCAACAC CTGCAACCCG TCCACCATTA CCGGCACTCT GTCCCGCCTG 
TCTCTGGATG AGGATGAGGA GCCCAAGTCC TGTCTCGTGG GTCCTGAGGG TGGCCTCAGC 
TTCTCACCAC TGCCTGGGAA CATCCTGGTA CCCATGGCAG CCTCACCAGG TCTAGGGGAG 
CCACCACCAC CCCAGGAGAC CAACCCTGTG TACATGTGTG GGGAGGGTGG CCTGCGGCAG 
TTGGACCTTA CATGGATACG GCAGAGTGAA CCAGGCTCTG AGGGGGACTA CATGGTTCTG 
CCCCGGCGGA CTTTGAGCCT GCAGCCTGGT GGTGGGGGTA CAGCGGGTGA GGAGGCCCCA 
AGGGCCCGGC CTGAGGGGAC CCCCCGGCGG GCTGCCAAAA CGGTAGCCCA CACTGAAGGC 
TACCCCAGCT TCCTGTCTGT GGAGCACTCG GGTCTAGGGC TGGGCCCTGC CTATGGGTCT 
CTCCAGAACC CGTATGGAAT GACCTTCCAA CCACCACCAC CAACACCCAG CGCCCGCCAA 
GTACCAGAGC CAGGAGAACG TAGCCGGACC ATGCCCCGTA CAGTGCCTGG TTCCACCATG 
AAGCTGGGCT CCCTTGAGCG AAAGAAGCTT CGGTATTCGG ACTTGGACTT TGAGAAGGTG 
ATGCACACTC GGAAACGGCA CTCGGAACTC TACCACGAAC TCAACCAGAA GTTCCACACT 
TTCGACCGCT ACCGTAGCCA GTCCTCAGCC AAGGAGAAAC CCAGCCCCCC CGGGGGACGC 
CCTGGCTTGT CCCAGCACAG GAGGCATCAA AGCTGGAGCA CCTTCAAATC TATGACACTG 
GGCTCACTGC CCCCCAAGCC CCGAGAACGG CTGGCCCTGC ACCGGACAGC AGCCTGGGAG 
CCCACAGAAC CGCCAGACGG CGACTTCCAG ACAGAGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011238815.1
CDS	16..4437
Translation	MTPACPLLLSVILSLRLATAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCTHFAPRLLPLDHYLVNFTCLRPGPEEATARAESEVGRPEEEEEEAAAAASGLELCGGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGANPATTTSPGPPVAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTVHGVWEEWGSWSLCSRSCGRGSRSRMRTCVPPQHGGKACEGPELQTKLCSMAACPVEGQWLEWGPWGPCSSSCANGTQQRSRKCSVAGPAWATCAGALTDTRECSNLDCPATDGKWGPWNAWSLCSKTCDTGWQRRFRMCQASGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKRAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDSENKDKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPISAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSSPGKPATPGAATAGSPGRGRGPGTVPPGPGHAHQRLLPADPEESSSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADTNSGDWNTESCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGAPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNILILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGVSDKSKKQRAGASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGGLSFSPLPGNILVPMAASPGLGEPPPPQETNPVYMCGEGGLRQLDLTWIRQSEPGSEGDYMVLPRRTLSLQPGGGGTAGEEAPRARPEGTPRRAAKTVAHTEGYPSFLSVEHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVPEPGERSRTMPRTVPGSTMKLGSLERKKLRYSDLDFEKVMHTRKRHSELYHELNQKFHTFDRYRSQSSAKEKPSPPGGRPGLSQHRRHQSWSTFKSMTLGSLPPKPRERLALHRTAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_011240513.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240513.1

ORF Insert Sequence:

ATGACCCCAG CCTGTCCCCT CTTACTGTCT GTGATTCTGT CCCTGCGCCT GGCCACGGCC 
TTCGACCCTG CCCCCAGTGC CTGCTCTGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTC 
TCGCTGCAGG ACCTCTTTCC CACCATCGCC TCGGGCTGCT CCTGGACCCT GGAGAACCCA 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGGC AGGAGCAGGT TTGCACACAC 
TTTGCCCCGC GCCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTTACCTG CCTGCGGCCT 
GGTCCAGAGG AAGCCACAGC CCGGGCTGAG TCGGAGGTGG GACGGCCAGA GGAGGAGGAG 
GAGGAGGCGG CGGCAGCAGC ATCAGGGTTG GAGTTGTGTG GTGGCTCAGG CCCCTTTACC 
TTTCTGCACT TCGACAAGAA CTTTGTGCAG CTGTGCCTGT CGGCTGAGCC CTCTGAGGCC 
CCTCGTCTGC TAGCGCCTGC TGCCCTGGCC TTCCGTTTTG TGGAGGTCTT GCTGATCAAC 
AACAACAACT CCAGCCAGTT CACCTGTGGT GTGCTCTGCC GCTGGAGTGA GGAGTGTGGC 
CGGGCTGCAG GCAGGGCTTG TGGCTTTGCA CAGCCAGGGT GTAGTTGTCC TGGGGAGGCA 
GGGGCCAACC CCGCCACCAC CACATCTCCG GGGCCTCCGG TTGCCCACAC CCTGTCCAAT 
GCCCTGGTGC CCGGGGGCCC AGCCCCTCCT GCTGAGGCCG ACTTGCACTC GGGGAGCAGC 
AATGACCTGT TCACCACCGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG 
AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTAT ACATGGCGCA GACAGTGCAC 
GGCGTGTGGG AGGAGTGGGG GTCCTGGAGC CTGTGCTCCC GCAGCTGCGG GCGGGGGTCT 
CGGAGCCGGA TGCGGACCTG CGTGCCCCCC CAGCACGGCG GCAAGGCCTG CGAGGGTCCC 
GAGCTGCAGA CTAAACTCTG CAGTATGGCC GCCTGCCCGG TGGAAGGCCA GTGGCTAGAA 
TGGGGTCCCT GGGGCCCATG CTCATCATCT TGTGCCAATG GGACCCAGCA GCGCAGCCGG 
AAATGCAGTG TGGCGGGTCC AGCCTGGGCC ACGTGCGCAG GTGCCCTCAC GGATACCCGT 
GAGTGCAGCA ATCTCGATTG CCCGGCCACT GACGGCAAGT GGGGGCCGTG GAACGCGTGG 
AGCCTGTGCT CCAAGACGTG TGACACGGGC TGGCAACGCC GCTTCCGCAT GTGCCAGGCT 
TCTGGCACAC AGGGCTACCC TTGCGAGGGC ACAGGAGAGG AGGTGAAACC CTGCAGTGAG 
AAGAGGTGTC CAGCCTTCCA TGAGATGTGC AGGGATGAGT ACGTGATGTT GATGACATGG 
AAGAGGGCGG CAGCTGGCGA GATCATTTAC AACAAGTGTC CCCCTAATGC CTCGGGTTCT 
GCTAGCCGCC GCTGTCTCCT CAGTGCCCAG GGCGTAGCAT ACTGGGGACT GCCCAGCTTT 
GCTCGTTGCA TATCCCATGA ATACCGCTAC CTGTACCTGT CACTTCGGGA ACACCTGGCT 
AAGGGCCAGC GCATGCTGGC AGGTGAGGGC ATGTCACAGG TGGTGCGGAG CCTGCAGGAG 
CTACTGGCAC GGCGCACTTA CTACAGCGGG GACCTGCTCT TCTCTGTGGA CATCCTAAGG 
AACGTCACTG ACACCTTCAA GAGGGCCACC TATGTCCCTT CCGCCGATGA CGTGCAGCGT 
TTCTTCCAGG TGGTGAGCTT CATGGTGGAT TCAGAAAACA AGGACAAATG GGATGATGCT 
CAGCAGGTGT CACCGGGCTC TGTGCACCTG CTGCGTGTTG TGGAAGATTT CATTCACCTC 
GTGGGCGACG CTCTCAAGGC CTTCCAGAGC TCTCTCATTG TCACGGACAA TCTGGTGATC 
AGCATTCAGA GAGAGCCTAT CTCCGCCGTG TCCAGTGACA TCACGTTTCC CATGCGGGGC 
CGCAGGGGCA TGAAGGACTG GGTGCGACAC TCAGAGGATC GTCTCTTTCT ACCCAAGGAG 
GTGCTCAGCC TGTCCTCCCC AGGAAAGCCA GCCACACCTG GGGCAGCCAC AGCAGGCAGC 
CCGGGCAGGG GGAGGGGCCC AGGAACGGTG CCCCCTGGCC CAGGCCACGC CCACCAGCGC 
CTTCTCCCAG CTGACCCCGA AGAGTCCTCC TCCTACTTTG TGATCGGTGC TGTGCTCTAC 
CGCACCCTTG GCCTCATCCT GCCGCCCCCC AGGCCTCCAC TTGCTGTCAC CTCCCGGGTG 
ATGACAGTGA CTGTGCGTCC CCCCACCCAG CCTCCAGCTG AGCCCCTCAT TACAGTGGAA 
CTCTCGTACA TCATCAATGG CACCACCGAT CCCCACTGTG CCAGCTGGGA CTACTCCAGA 
GCAGATACCA ACTCGGGGGA CTGGAACACT GAGAGCTGCC AGACCTTGGA GACCCAGGCG 
GCTCACACCC GCTGCCAGTG CCAGCACCTG TCCACCTTTG CCGTCCTGGC CCAGCCACCC 
AAGGACCTGA CCCTGGAGCT GGCAGGTGCT CCCTCTGTCC CCCTGGTGAT CGGCTGTGCA 
GTGTCCTGCA TGGCTCTGCT CACCCTGCTG GCCATCTATG CAGCCTTCTG GAGGTTTATA 
AAATCAGAAC GCTCCATCAT CTTGCTGAAC TTCTGCCTGT CCATCCTGGC TTCCAACATT 
CTGATCCTGG TGGGCCAGTC CCGGGTGCTG AGCAAGGGCG TATGCACCAT GACGGCTGCC 
TTCCTACACT TCTTCTTTCT GTCCTCCTTT TGCTGGGTGC TTACAGAGGC CTGGCAATCC 
TATCTGGCTG TCATCGGGCG GATGCGCACC CGCCTGGTTC GCAAGCGCTT CCTCTGCCTG 
GGCTGGGGTC TACCTGCCCT GGTGGTGGCT GTGTCTGTTG GCTTTACTCG CACCAAAGGA 
TATGGTACAT CCAGCTACTG CTGGCTGTCC CTAGAGGGCG GCCTGCTCTA TGCCTTCGTG 
GGTCCAGCAG CAGTCATTGT CCTGGTGAAC ATGCTCATCG GGATTATCGT CTTCAACAAG 
CTCATGGCTC GCGATGGCGT CTCAGACAAA TCTAAGAAGC AGAGGGCTGG GGCTTCACTC 
TGGAGTTCCT GCGTGGTACT GCCTCTCCTG GCGCTTACCT GGATGTCTGC CGTCCTGGCC 
ATGACAGATC GCCGCTCCGT CCTCTTCCAG GCACTCTTTG CCGTTTTCAA CTCTGCACAA 
GGCTTTGTCA TCACCGCTGT GCACTGCTTC CTGCGCCGAG AGGTCCAGGA TGTGGTAAAG 
TGTCAGATGG GTGTGTGTCG GGCTGATGAG AGTGAAGACT CCCCAGACTC GTGCAAGAAC 
GGGCAGCTGC AGATCCTGTC AGACTTTGAA AAGGACGTGG ATCTGGCTTG TCAGACAGTT 
CTGTTCAAGG AGGTCAACAC CTGCAACCCG TCCACCATTA CCGGCACTCT GTCCCGCCTG 
TCTCTGGATG AGGATGAGGA GCCCAAGTCC TGTCTCGTGG GTCCTGAGGG TGGCCTCAGC 
TTCTCACCAC TGCCTGGGAA CATCCTGGTA CCCATGGCAG CCTCACCAGG TCTAGGGGAG 
CCACCACCAC CCCAGGAGAC CAACCCTGTG TACATGTGTG GGGAGGGTGG CCTGCGGCAG 
TTGGACCTTA CATGGATACG GCAGAGTGAA CCAGGCTCTG AGGGGGACTA CATGGTTCTG 
CCCCGGCGGA CTTTGAGCCT GCAGCCTGGT GGTGGGGGTA CAGCGGGTGA GGAGGCCCCA 
AGGGCCCGGC CTGAGGGGAC CCCCCGGCGG GCTGCCAAAA CGGTAGCCCA CACTGAAGGC 
TACCCCAGCT TCCTGTCTGT GGAGCACTCG GGTCTAGGGC TGGGCCCTGC CTATGGGTCT 
CTCCAGAACC CGTATGGAAT GACCTTCCAA CCACCACCAC CAACACCCAG CGCCCGCCAA 
GTACCAGAGC CAGGAGAACG TAGCCGGACC ATGCCCCGTA CAGTGCCTGG TTCCACCATG 
AAGCTGGGCT CCCTTGAGCG AAAGAAGCTT CGGTATTCGG ACTTGGACTT TGAGAAGGTG 
ATGCACACTC GGAAACGGCA CTCGGAACTC TACCACGAAC TCAACCAGAA GTTCCACACT 
TTCGACCGCT ACCGTAGCCA GTCCTCAGCC AAGGAGAAAC CCAGCCCCCC CGGGGGACGC 
CCTGGCTTGT CCCAGCACAG GAGGCATCAA AGCTGGAGCA CCTTCAAATC TATGACACTG 
GGCTCACTGC CCCCCAAGCC CCGAGAACGG CTGGCCCTGC ACCGGACAGC AGCCTGGGAG 
CCCACAGAAC CGCCAGACGG CGACTTCCAG ACAGAGGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adgrb2 ( XM_011240514.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011238816.1 Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant X9, mRNA.

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CloneID	OMu73521
Clone ID Related Accession (Same CDS sequence)	XM_011240514.1 , XM_030253459.1
Accession Version	XM_011240514.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	adhesion G protein-coupled receptor B2 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCAG CCTGTCCCCT CTTACTGTCT GTGATTCTGT CCCTGCGCCT GGCCACGGCC 
TTCGACCCTG CCCCCAGTGC CTGCTCTGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTC 
TCGCTGCAGG ACCTCTTTCC CACCATCGCC TCGGGCTGCT CCTGGACCCT GGAGAACCCA 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGGC AGGAGCAGGT TTGCACACAC 
TTTGCCCCGC GCCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTTACCTG CCTGCGGCCT 
GGTCCAGAGG AAGCCACAGC CCGGGCTGAG TCGGAGGTGG GACGGCCAGA GGAGGAGGAG 
GAGGAGGCGG CGGCAGCAGC ATCAGGGTTG GAGTTGTGTG GTGGCTCAGG CCCCTTTACC 
TTTCTGCACT TCGACAAGAA CTTTGTGCAG CTGTGCCTGT CGGCTGAGCC CTCTGAGGCC 
CCTCGTCTGC TAGCGCCTGC TGCCCTGGCC TTCCGTTTTG TGGAGGTCTT GCTGATCAAC 
AACAACAACT CCAGCCAGTT CACCTGTGGT GTGCTCTGCC GCTGGAGTGA GGAGTGTGGC 
CGGGCTGCAG GCAGGGCTTG TGGCTTTGCA CAGCCAGGGT GTAGTTGTCC TGGGGAGGCA 
GGGGCCAACC CCGCCACCAC CACATCTCCG GGGCCTCCGG TTGCCCACAC CCTGTCCAAT 
GCCCTGGTGC CCGGGGGCCC AGCCCCTCCT GCTGAGGCCG ACTTGCACTC GGGGAGCAGC 
AATGACCTGT TCACCACCGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG 
AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTAT ACATGGCGCA GACAGTGGAA 
GGCCAGTGGC TAGAATGGGG TCCCTGGGGC CCATGCTCAT CATCTTGTGC CAATGGGACC 
CAGCAGCGCA GCCGGAAATG CAGTGTGGCG GGTCCAGCCT GGGCCACGTG CGCAGGTGCC 
CTCACGGATA CCCGTGAGTG CAGCAATCTC GATTGCCCGG CCACTGACGG CAAGTGGGGG 
CCGTGGAACG CGTGGAGCCT GTGCTCCAAG ACGTGTGACA CGGGCTGGCA ACGCCGCTTC 
CGCATGTGCC AGGCTTCTGG CACACAGGGC TACCCTTGCG AGGGCACAGG AGAGGAGGTG 
AAACCCTGCA GTGAGAAGAG GTGTCCAGCC TTCCATGAGA TGTGCAGGGA TGAGTACGTG 
ATGTTGATGA CATGGAAGAG GGCGGCAGCT GGCGAGATCA TTTACAACAA GTGTCCCCCT 
AATGCCTCGG GTTCTGCTAG CCGCCGCTGT CTCCTCAGTG CCCAGGGCGT AGCATACTGG 
GGACTGCCCA GCTTTGCTCG TTGCATATCC CATGAATACC GCTACCTGTA CCTGTCACTT 
CGGGAACACC TGGCTAAGGG CCAGCGCATG CTGGCAGGTG AGGGCATGTC ACAGGTGGTG 
CGGAGCCTGC AGGAGCTACT GGCACGGCGC ACTTACTACA GCGGGGACCT GCTCTTCTCT 
GTGGACATCC TAAGGAACGT CACTGACACC TTCAAGAGGG CCACCTATGT CCCTTCCGCC 
GATGACGTGC AGCGTTTCTT CCAGGTGGTG AGCTTCATGG TGGATTCAGA AAACAAGGAC 
AAATGGGATG ATGCTCAGCA GGTGTCACCG GGCTCTGTGC ACCTGCTGCG TGTTGTGGAA 
GATTTCATTC ACCTCGTGGG CGACGCTCTC AAGGCCTTCC AGAGCTCTCT CATTGTCACG 
GACAATCTGG TGATCAGCAT TCAGAGAGAG CCTATCTCCG CCGTGTCCAG TGACATCACG 
TTTCCCATGC GGGGCCGCAG GGGCATGAAG GACTGGGTGC GACACTCAGA GGATCGTCTC 
TTTCTACCCA AGGAGGTGCT CAGCCTGTCC TCCCCAGGAA AGCCAGCCAC ACCTGGGGCA 
GCCACAGCAG GCAGCCCGGG CAGGGGGAGG GGCCCAGGAA CGGTGCCCCC TGGCCCAGGC 
CACGCCCACC AGCGCCTTCT CCCAGCTGAC CCCGAAGAGT CCTCCTCCTA CTTTGTGATC 
GGTGCTGTGC TCTACCGCAC CCTTGGCCTC ATCCTGCCGC CCCCCAGGCC TCCACTTGCT 
GTCACCTCCC GGGTGATGAC AGTGACTGTG CGTCCCCCCA CCCAGCCTCC AGCTGAGCCC 
CTCATTACAG TGGAACTCTC GTACATCATC AATGGCACCA CCGATCCCCA CTGTGCCAGC 
TGGGACTACT CCAGAGCAGA TACCAACTCG GGGGACTGGA ACACTGAGAG CTGCCAGACC 
TTGGAGACCC AGGCGGCTCA CACCCGCTGC CAGTGCCAGC ACCTGTCCAC CTTTGCCGTC 
CTGGCCCAGC CACCCAAGGA CCTGACCCTG GAGCTGGCAG GTGCTCCCTC TGTCCCCCTG 
GTGATCGGCT GTGCAGTGTC CTGCATGGCT CTGCTCACCC TGCTGGCCAT CTATGCAGCC 
TTCTGGAGGT TTATAAAATC AGAACGCTCC ATCATCTTGC TGAACTTCTG CCTGTCCATC 
CTGGCTTCCA ACATTCTGAT CCTGGTGGGC CAGTCCCGGG TGCTGAGCAA GGGCGTATGC 
ACCATGACGG CTGCCTTCCT ACACTTCTTC TTTCTGTCCT CCTTTTGCTG GGTGCTTACA 
GAGGCCTGGC AATCCTATCT GGCTGTCATC GGGCGGATGC GCACCCGCCT GGTTCGCAAG 
CGCTTCCTCT GCCTGGGCTG GGGTCTACCT GCCCTGGTGG TGGCTGTGTC TGTTGGCTTT 
ACTCGCACCA AAGGATATGG TACATCCAGC TACTGCTGGC TGTCCCTAGA GGGCGGCCTG 
CTCTATGCCT TCGTGGGTCC AGCAGCAGTC ATTGTCCTGG TGAACATGCT CATCGGGATT 
ATCGTCTTCA ACAAGCTCAT GGCTCGCGAT GGCGTCTCAG ACAAATCTAA GAAGCAGAGG 
GCTGGGTCGG AGCGGTGCCC CTGGGCCAGC CTGCTCCTTC CCTGCTCAGC GTGTGGAGCG 
GTCCCCAGCC CCCTGCTCAG CTCAGCCTCG GCCAGGAATG CCATCCGACT GTCTTGGAAT 
CTCTGGGGAT ATGGCTCTCA ACTGTGTCTT TTTCCAAAGC TTCCAAGGGC TTCACTCTGG 
AGTTCCTGCG TGGTACTGCC TCTCCTGGCG CTTACCTGGA TGTCTGCCGT CCTGGCCATG 
ACAGATCGCC GCTCCGTCCT CTTCCAGGCA CTCTTTGCCG TTTTCAACTC TGCACAAGGC 
TTTGTCATCA CCGCTGTGCA CTGCTTCCTG CGCCGAGAGG TCCAGGATGT GGTAAAGTGT 
CAGATGGGTG TGTGTCGGGC TGATGAGAGT GAAGACTCCC CAGACTCGTG CAAGAACGGG 
CAGCTGCAGA TCCTGTCAGA CTTTGAAAAG GACGTGGATC TGGCTTGTCA GACAGTTCTG 
TTCAAGGAGG TCAACACCTG CAACCCGTCC ACCATTACCG GCACTCTGTC CCGCCTGTCT 
CTGGATGAGG ATGAGGAGCC CAAGTCCTGT CTCGTGGGTC CTGAGGGTGG CCTCAGCTTC 
TCACCACTGC CTGGGAACAT CCTGGTACCC ATGGCAGCCT CACCAGGTCT AGGGGAGCCA 
CCACCACCCC AGGAGACCAA CCCTGTGTAC ATGTGTGGGG AGGGTGGCCT GCGGCAGTTG 
GACCTTACAT GGATACGGCA GAGTGAACCA GGCTCTGAGG GGGACTACAT GGTTCTGCCC 
CGGCGGACTT TGAGCCTGCA GCCTGGTGGT GGGGGTACAG CGGGTGAGGA GGCCCCAAGG 
GCCCGGCCTG AGGGGACCCC CCGGCGGGCT GCCAAAACGG TAGCCCACAC TGAAGGCTAC 
CCCAGCTTCC TGTCTGTGGA GCACTCGGGT CTAGGGCTGG GCCCTGCCTA TGGGTCTCTC 
CAGAACCCGT ATGGAATGAC CTTCCAACCA CCACCACCAA CACCCAGCGC CCGCCAAGTA 
CCAGAGCCAG GAGAACGTAG CCGGACCATG CCCCGTACAG TGCCTGGTTC CACCATGAAG 
CTGGGCTCCC TTGAGCGAAA GAAGCTTCGG TATTCGGACT TGGACTTTGA GAAGGTGATG 
CACACTCGGA AACGGCACTC GGAACTCTAC CACGAACTCA ACCAGAAGTT CCACACTTTC 
GACCGCTACC GTAGCCAGTC CTCAGCCAAG GAGAAACCCA GCCCCCCCGG GGGACGCCCT 
GGCTTGTCCC AGCACAGGAG GCATCAAAGC TGGAGCACCT TCAAATCTAT GACACTGGGC 
TCACTGCCCC CCAAGCCCCG AGAACGGCTG GCCCTGCACC GGACAGCAGC CTGGGAGCCC 
ACAGAACCGC CAGACGGCGA CTTCCAGACA GAGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011238816.1
CDS	16..4434
Translation	MTPACPLLLSVILSLRLATAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLRFNRQEQVCTHFAPRLLPLDHYLVNFTCLRPGPEEATARAESEVGRPEEEEEEAAAAASGLELCGGSGPFTFLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFAQPGCSCPGEAGANPATTTSPGPPVAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKVKTQWPRSADEPGLYMAQTVEGQWLEWGPWGPCSSSCANGTQQRSRKCSVAGPAWATCAGALTDTRECSNLDCPATDGKWGPWNAWSLCSKTCDTGWQRRFRMCQASGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKRAAAGEIIYNKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQELLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDSENKDKWDDAQQVSPGSVHLLRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPISAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKEVLSLSSPGKPATPGAATAGSPGRGRGPGTVPPGPGHAHQRLLPADPEESSSYFVIGAVLYRTLGLILPPPRPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADTNSGDWNTESCQTLETQAAHTRCQCQHLSTFAVLAQPPKDLTLELAGAPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLNFCLSILASNILILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCLGWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGVSDKSKKQRAGSERCPWASLLLPCSACGAVPSPLLSSASARNAIRLSWNLWGYGSQLCLFPKLPRASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVKCQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKSCLVGPEGGLSFSPLPGNILVPMAASPGLGEPPPPQETNPVYMCGEGGLRQLDLTWIRQSEPGSEGDYMVLPRRTLSLQPGGGGTAGEEAPRARPEGTPRRAAKTVAHTEGYPSFLSVEHSGLGLGPAYGSLQNPYGMTFQPPPPTPSARQVPEPGERSRTMPRTVPGSTMKLGSLERKKLRYSDLDFEKVMHTRKRHSELYHELNQKFHTFDRYRSQSSAKEKPSPPGGRPGLSQHRRHQSWSTFKSMTLGSLPPKPRERLALHRTAAWEPTEPPDGDFQTEV

Target ORF information:

RefSeq Version	XM_011240514.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus adhesion G protein-coupled receptor B2 (Adgrb2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240514.1

ORF Insert Sequence:

ATGACCCCAG CCTGTCCCCT CTTACTGTCT GTGATTCTGT CCCTGCGCCT GGCCACGGCC 
TTCGACCCTG CCCCCAGTGC CTGCTCTGCC CTGGCCTCGG GCGTGCTCTA CGGGGCCTTC 
TCGCTGCAGG ACCTCTTTCC CACCATCGCC TCGGGCTGCT CCTGGACCCT GGAGAACCCA 
GACCCCACCA AGTACTCCCT CTACCTGCGC TTCAACCGGC AGGAGCAGGT TTGCACACAC 
TTTGCCCCGC GCCTGCTGCC CCTGGACCAC TACCTGGTCA ACTTTACCTG CCTGCGGCCT 
GGTCCAGAGG AAGCCACAGC CCGGGCTGAG TCGGAGGTGG GACGGCCAGA GGAGGAGGAG 
GAGGAGGCGG CGGCAGCAGC ATCAGGGTTG GAGTTGTGTG GTGGCTCAGG CCCCTTTACC 
TTTCTGCACT TCGACAAGAA CTTTGTGCAG CTGTGCCTGT CGGCTGAGCC CTCTGAGGCC 
CCTCGTCTGC TAGCGCCTGC TGCCCTGGCC TTCCGTTTTG TGGAGGTCTT GCTGATCAAC 
AACAACAACT CCAGCCAGTT CACCTGTGGT GTGCTCTGCC GCTGGAGTGA GGAGTGTGGC 
CGGGCTGCAG GCAGGGCTTG TGGCTTTGCA CAGCCAGGGT GTAGTTGTCC TGGGGAGGCA 
GGGGCCAACC CCGCCACCAC CACATCTCCG GGGCCTCCGG TTGCCCACAC CCTGTCCAAT 
GCCCTGGTGC CCGGGGGCCC AGCCCCTCCT GCTGAGGCCG ACTTGCACTC GGGGAGCAGC 
AATGACCTGT TCACCACCGA GATGAGATAT GGTGAGGAGC CGGAAGAGGA ACCGAAGGTG 
AAAACCCAGT GGCCAAGGTC TGCAGATGAG CCTGGGCTAT ACATGGCGCA GACAGTGGAA 
GGCCAGTGGC TAGAATGGGG TCCCTGGGGC CCATGCTCAT CATCTTGTGC CAATGGGACC 
CAGCAGCGCA GCCGGAAATG CAGTGTGGCG GGTCCAGCCT GGGCCACGTG CGCAGGTGCC 
CTCACGGATA CCCGTGAGTG CAGCAATCTC GATTGCCCGG CCACTGACGG CAAGTGGGGG 
CCGTGGAACG CGTGGAGCCT GTGCTCCAAG ACGTGTGACA CGGGCTGGCA ACGCCGCTTC 
CGCATGTGCC AGGCTTCTGG CACACAGGGC TACCCTTGCG AGGGCACAGG AGAGGAGGTG 
AAACCCTGCA GTGAGAAGAG GTGTCCAGCC TTCCATGAGA TGTGCAGGGA TGAGTACGTG 
ATGTTGATGA CATGGAAGAG GGCGGCAGCT GGCGAGATCA TTTACAACAA GTGTCCCCCT 
AATGCCTCGG GTTCTGCTAG CCGCCGCTGT CTCCTCAGTG CCCAGGGCGT AGCATACTGG 
GGACTGCCCA GCTTTGCTCG TTGCATATCC CATGAATACC GCTACCTGTA CCTGTCACTT 
CGGGAACACC TGGCTAAGGG CCAGCGCATG CTGGCAGGTG AGGGCATGTC ACAGGTGGTG 
CGGAGCCTGC AGGAGCTACT GGCACGGCGC ACTTACTACA GCGGGGACCT GCTCTTCTCT 
GTGGACATCC TAAGGAACGT CACTGACACC TTCAAGAGGG CCACCTATGT CCCTTCCGCC 
GATGACGTGC AGCGTTTCTT CCAGGTGGTG AGCTTCATGG TGGATTCAGA AAACAAGGAC 
AAATGGGATG ATGCTCAGCA GGTGTCACCG GGCTCTGTGC ACCTGCTGCG TGTTGTGGAA 
GATTTCATTC ACCTCGTGGG CGACGCTCTC AAGGCCTTCC AGAGCTCTCT CATTGTCACG 
GACAATCTGG TGATCAGCAT TCAGAGAGAG CCTATCTCCG CCGTGTCCAG TGACATCACG 
TTTCCCATGC GGGGCCGCAG GGGCATGAAG GACTGGGTGC GACACTCAGA GGATCGTCTC 
TTTCTACCCA AGGAGGTGCT CAGCCTGTCC TCCCCAGGAA AGCCAGCCAC ACCTGGGGCA 
GCCACAGCAG GCAGCCCGGG CAGGGGGAGG GGCCCAGGAA CGGTGCCCCC TGGCCCAGGC 
CACGCCCACC AGCGCCTTCT CCCAGCTGAC CCCGAAGAGT CCTCCTCCTA CTTTGTGATC 
GGTGCTGTGC TCTACCGCAC CCTTGGCCTC ATCCTGCCGC CCCCCAGGCC TCCACTTGCT 
GTCACCTCCC GGGTGATGAC AGTGACTGTG CGTCCCCCCA CCCAGCCTCC AGCTGAGCCC 
CTCATTACAG TGGAACTCTC GTACATCATC AATGGCACCA CCGATCCCCA CTGTGCCAGC 
TGGGACTACT CCAGAGCAGA TACCAACTCG GGGGACTGGA ACACTGAGAG CTGCCAGACC 
TTGGAGACCC AGGCGGCTCA CACCCGCTGC CAGTGCCAGC ACCTGTCCAC CTTTGCCGTC 
CTGGCCCAGC CACCCAAGGA CCTGACCCTG GAGCTGGCAG GTGCTCCCTC TGTCCCCCTG 
GTGATCGGCT GTGCAGTGTC CTGCATGGCT CTGCTCACCC TGCTGGCCAT CTATGCAGCC 
TTCTGGAGGT TTATAAAATC AGAACGCTCC ATCATCTTGC TGAACTTCTG CCTGTCCATC 
CTGGCTTCCA ACATTCTGAT CCTGGTGGGC CAGTCCCGGG TGCTGAGCAA GGGCGTATGC 
ACCATGACGG CTGCCTTCCT ACACTTCTTC TTTCTGTCCT CCTTTTGCTG GGTGCTTACA 
GAGGCCTGGC AATCCTATCT GGCTGTCATC GGGCGGATGC GCACCCGCCT GGTTCGCAAG 
CGCTTCCTCT GCCTGGGCTG GGGTCTACCT GCCCTGGTGG TGGCTGTGTC TGTTGGCTTT 
ACTCGCACCA AAGGATATGG TACATCCAGC TACTGCTGGC TGTCCCTAGA GGGCGGCCTG 
CTCTATGCCT TCGTGGGTCC AGCAGCAGTC ATTGTCCTGG TGAACATGCT CATCGGGATT 
ATCGTCTTCA ACAAGCTCAT GGCTCGCGAT GGCGTCTCAG ACAAATCTAA GAAGCAGAGG 
GCTGGGTCGG AGCGGTGCCC CTGGGCCAGC CTGCTCCTTC CCTGCTCAGC GTGTGGAGCG 
GTCCCCAGCC CCCTGCTCAG CTCAGCCTCG GCCAGGAATG CCATCCGACT GTCTTGGAAT 
CTCTGGGGAT ATGGCTCTCA ACTGTGTCTT TTTCCAAAGC TTCCAAGGGC TTCACTCTGG 
AGTTCCTGCG TGGTACTGCC TCTCCTGGCG CTTACCTGGA TGTCTGCCGT CCTGGCCATG 
ACAGATCGCC GCTCCGTCCT CTTCCAGGCA CTCTTTGCCG TTTTCAACTC TGCACAAGGC 
TTTGTCATCA CCGCTGTGCA CTGCTTCCTG CGCCGAGAGG TCCAGGATGT GGTAAAGTGT 
CAGATGGGTG TGTGTCGGGC TGATGAGAGT GAAGACTCCC CAGACTCGTG CAAGAACGGG 
CAGCTGCAGA TCCTGTCAGA CTTTGAAAAG GACGTGGATC TGGCTTGTCA GACAGTTCTG 
TTCAAGGAGG TCAACACCTG CAACCCGTCC ACCATTACCG GCACTCTGTC CCGCCTGTCT 
CTGGATGAGG ATGAGGAGCC CAAGTCCTGT CTCGTGGGTC CTGAGGGTGG CCTCAGCTTC 
TCACCACTGC CTGGGAACAT CCTGGTACCC ATGGCAGCCT CACCAGGTCT AGGGGAGCCA 
CCACCACCCC AGGAGACCAA CCCTGTGTAC ATGTGTGGGG AGGGTGGCCT GCGGCAGTTG 
GACCTTACAT GGATACGGCA GAGTGAACCA GGCTCTGAGG GGGACTACAT GGTTCTGCCC 
CGGCGGACTT TGAGCCTGCA GCCTGGTGGT GGGGGTACAG CGGGTGAGGA GGCCCCAAGG 
GCCCGGCCTG AGGGGACCCC CCGGCGGGCT GCCAAAACGG TAGCCCACAC TGAAGGCTAC 
CCCAGCTTCC TGTCTGTGGA GCACTCGGGT CTAGGGCTGG GCCCTGCCTA TGGGTCTCTC 
CAGAACCCGT ATGGAATGAC CTTCCAACCA CCACCACCAA CACCCAGCGC CCGCCAAGTA 
CCAGAGCCAG GAGAACGTAG CCGGACCATG CCCCGTACAG TGCCTGGTTC CACCATGAAG 
CTGGGCTCCC TTGAGCGAAA GAAGCTTCGG TATTCGGACT TGGACTTTGA GAAGGTGATG 
CACACTCGGA AACGGCACTC GGAACTCTAC CACGAACTCA ACCAGAAGTT CCACACTTTC 
GACCGCTACC GTAGCCAGTC CTCAGCCAAG GAGAAACCCA GCCCCCCCGG GGGACGCCCT 
GGCTTGTCCC AGCACAGGAG GCATCAAAGC TGGAGCACCT TCAAATCTAT GACACTGGGC 
TCACTGCCCC CCAAGCCCCG AGAACGGCTG GCCCTGCACC GGACAGCAGC CTGGGAGCCC 
ACAGAACCGC CAGACGGCGA CTTCCAGACA GAGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Whole exome sequencing reveals a functional mutation in the GAIN domain of the Bai2 receptor underlying a forward mutagenesis hyperactivity QTL.
Mammalian genome : official journal of the International Mammalian Genome Society28(11-12)465-475(2017 Dec)
Speca DJ,Trimmer JS,Peterson AS,D?az E

International Union of Basic and Clinical Pharmacology. XCIV. Adhesion G protein-coupled receptors.
Pharmacological reviews67(2)338-67(2015)
Hamann J,Aust G,Ara? D,Engel FB,Formstone C,Fredriksson R,Hall RA,Harty BL,Kirchhoff C,Knapp B,Krishnan A,Liebscher I,Lin HH,Martinelli DC,Monk KR,Peeters MC,Piao X,Pr?mel S,Sch?neberg T,Schwartz TW,Singer K,Stacey M,Ushkaryov YA,Vallon M,Wolfrum U,Wright MW,Xu L,Langenhan T,Schi?th HB

Anterograde C1ql1 signaling is required in order to determine and maintain a single-winner climbing fiber in the mouse cerebellum.
Neuron85(2)316-29(2015 Jan)
Kakegawa W,Mitakidis N,Miura E,Abe M,Matsuda K,Takeo YH,Kohda K,Motohashi J,Takahashi A,Nagao S,Muramatsu S,Watanabe M,Sakimura K,Aricescu AR,Yuzaki M

Calsyntenins function as synaptogenic adhesion molecules in concert with neurexins.
Cell reports6(6)1096-1109(2014 Mar)
Um JW,Pramanik G,Ko JS,Song MY,Lee D,Kim H,Park KS,S?dhof TC,Tabuchi K,Ko J

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

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Adgrb2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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