Nt5c1a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nt5c1a
Entrez Gene ID	230718
Full Name	5'-nucleotidase, cytosolic IA
Synonyms	Cn1a,Gm49
General protein information	Preferred Names 5'-nucleotidase, cytosolic IA Names cytosolic 5'-nucleotidase 1A cN-IA cytosolic 5' nucleotidase, type 1A cytosolic 5'-nucleotidase IA
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 D2.2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001085502.1	NP_001078971.1	cytosolic 5'-nucleotidase 1A
NM_001085502.2	NP_001078971.1	cytosolic 5'-nucleotidase 1A

Pathways from BioSystems

Homologies

Bos taurus (cattle)	NT5C1A	NP_001192475.1
Rattus norvegicus (Norway rat)	Nt5c1a	NP_001101446.1
Gallus gallus (chicken)	NT5C1A	XP_417822.3
Mus musculus (house mouse)	Nt5c1a	NP_001078971.1
Danio rerio (zebrafish)	LOC799449	XP_005158936.1
Pan troglodytes (chimpanzee)	NT5C1A	XP_524674.2
	NT5C1A	XP_001113402.1
Canis lupus familiaris (dog)	NT5C1A	XP_539580.3
Danio rerio (zebrafish)	nt5c1a	NP_001122185.1
Homo sapiens (human)	NT5C1A	NP_115915.1
Xenopus tropicalis (tropical clawed frog)	LOC100495335	XP_002941700.1

Gene Ontology
Bibliography

Related articles in PubMed

Effects of genetic deletion of soluble 5'-nucleotidases NT5C1A and NT5C2 on AMPK activation and nucleotide levels in contracting mouse skeletal muscles.
Kviklyte S, Vertommen D, Yerna X, Anders?n H, Xu X, Gailly P, Bohlooly-Y M, Oscarsson J, Rider MH
American journal of physiology. Endocrinology and metabolism313(1)E48-E62(2017 Jul)

Suppression of 5'-nucleotidase enzymes promotes AMP-activated protein kinase (AMPK) phosphorylation and metabolism in human and mouse skeletal muscle.
Kulkarni SS, Karlsson HK, Szekeres F, Chibalin AV, Krook A, Zierath JR
The Journal of biological chemistry286(40)34567-74(2011 Oct)

Gene structure and evolution of testicular haploid germ cell-specific genes, Oxct2a and Oxct2b.
Onishi M, Yasunaga T, Tanaka H, Nishimune Y, Nozaki M
Genomics83(4)647-57(2004 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results on muscles from the NT5C knockout mice contradict previous findings where AMP levels and AMPK activity were shown to be modulated by NT5C enzymes.
Title: Effects of genetic deletion of soluble 5'-nucleotidases NT5C1A and NT5C2 on AMPK activation and nucleotide levels in contracting mouse skeletal muscles.

NT5C1A suppression promotes AMP-activated protein kinase (AMPK) phosphorylation and metabolism in human and mouse skeletal muscle
Title: Suppression of 5'-nucleotidase enzymes promotes AMP-activated protein kinase (AMPK) phosphorylation and metabolism in human and mouse skeletal muscle.

The following Nt5c1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nt5c1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu02976	NM_001085502.2 Latest version!	Mus musculus 5'-nucleotidase, cytosolic IA (Nt5c1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$237.30 $339.00
View Old Accession Versions >>
OMu02976	NM_001085502.1	Mus musculus 5'-nucleotidase, cytosolic IA (Nt5c1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$237.30 $339.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nt5c1a ( NM_001085502.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001078971.1 Mus musculus 5'-nucleotidase, cytosolic IA (Nt5c1a), mRNA.

Price & Availability↑

CloneID	OMu02976
Clone ID Related Accession (Same CDS sequence)	NM_001085502.1 , NM_001085502.2
Accession Version	NM_001085502.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1098bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-20
Organism	Mus musculus(house mouse)
Product	cytosolic 5'-nucleotidase 1A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL606934.12. On or before Jul 25, 2007 this sequence version replaced XM_907862.2, XM_112246.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC147308.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849379 [ECO:0000348] ##Evidence-Data-END##

ATGGAACCAG GGCAGCCCCG GGAGGCCCGC GAACCCGGGC CAGGGGCAGA GACCGCCGCG 
GTACCGCGCT GGGAGGAAGC CAAGACTTTC TATGACAACC TCTCGTCCAA GAAGAAACCC 
AAATCGCCAA AGCCTCAGAA TGCGGTCACC ATCGCGGTGT CCTCCAGAGC CTTGTTCCGA 
ATGGATGAGG AACAGAGGAT CTACACGGAG CAGGGTGTCG AGGAGTACGT GCGATACCAG 
CTGGAGCACG AGAATGAGCC CTTCAGCCCC GGGCCTGCCT TCCCCTTTGT GAAGGCCCTG 
GAGGCTGTGA ACAAGCGACT TCGGGAGTTG TACCCTGATA GCGAAGATAT CTTTGACATT 
GTCCTCATGA CCAACAACCA TGCTCAGGTG GGAGTTCGTC TCATCAACAG TATCAACCAT 
TATGACCTGT TCATTGAGCG ATTCTGCATG ACAGGTGGGA ATAGCCCCAT CTGCTACCTC 
AAGGCCTACC ACACCAACCT CTACTTGTCA GCCGATGCAG ACAAAGTGCG AGAAGCCATC 
GATGAGGGGA TTGCAGCTGC CACCATCTTC AGCCCCAGCA GGGATGTGGT TGTGTCCCAG 
AGTCAGCTTC GAGTGGCCTT CGATGGGGAC GCAGTACTCT TCTCCGATGA GTCAGAGCGC 
ATCGTCAAGG CCCATGGGCT GGACAGATTC TTTGAGCATG AGAAGGCCCA CGAGAACAAA 
CCTTTGGCGC AGGGCCCCCT AAAGGGCTTC CTGGAAGCAC TGGGTAGACT GCAAAAGAAA 
TTCTACTCCA AGGGCCTGCG TCTGGAGTGC CCGATTCGCA CGTATTTGGT GACCGCGCGA 
AGTGCAGCCA GTTCCGGAGC CCGGGCCCTC AAGACCCTGC GCAGCTGGGG CCTGGAGACG 
GATGAGGCCT TATTCCTAGC TGGAGCACCC AAGGGTCCCC TTCTTGAGAA GATCCGTCCA 
CACATCTTCT TTGATGACCA GATGTTCCAC GTGGCTGGAG CTCAGGAGAT GGGCACGGTG 
GCCGCCCATG TGCCTTATGG TGTGGCTCAG AATCCCCGGC GGGCTGCCGC TGCGAAGCAG 
TCATTAGGTG CACAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001078971.1
CDS	6..1103
Translation	MEPGQPREAREPGPGAETAAVPRWEEAKTFYDNLSSKKKPKSPKPQNAVTIAVSSRALFRMDEEQRIYTEQGVEEYVRYQLEHENEPFSPGPAFPFVKALEAVNKRLRELYPDSEDIFDIVLMTNNHAQVGVRLINSINHYDLFIERFCMTGGNSPICYLKAYHTNLYLSADADKVREAIDEGIAAATIFSPSRDVVVSQSQLRVAFDGDAVLFSDESERIVKAHGLDRFFEHEKAHENKPLAQGPLKGFLEALGRLQKKFYSKGLRLECPIRTYLVTARSAASSGARALKTLRSWGLETDEALFLAGAPKGPLLEKIRPHIFFDDQMFHVAGAQEMGTVAAHVPYGVAQNPRRAAAAKQSLGAQ

Target ORF information:

RefSeq Version	NM_001085502.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus 5'-nucleotidase, cytosolic IA (Nt5c1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001085502.1

ORF Insert Sequence:

ATGGAACCAG GGCAGCCCCG GGAGGCCCGC GAACCCGGGC CAGGGGCAGA GACCGCCGCG 
GTACCGCGCT GGGAGGAAGC CAAGACTTTC TATGACAACC TCTCGTCCAA GAAGAAACCC 
AAATCGCCAA AGCCTCAGAA TGCGGTCACC ATCGCGGTGT CCTCCAGAGC CTTGTTCCGA 
ATGGATGAGG AACAGAGGAT CTACACGGAG CAGGGTGTCG AGGAGTACGT GCGATACCAG 
CTGGAGCACG AGAATGAGCC CTTCAGCCCC GGGCCTGCCT TCCCCTTTGT GAAGGCCCTG 
GAGGCTGTGA ACAAGCGACT TCGGGAGTTG TACCCTGATA GCGAAGATAT CTTTGACATT 
GTCCTCATGA CCAACAACCA TGCTCAGGTG GGAGTTCGTC TCATCAACAG TATCAACCAT 
TATGACCTGT TCATTGAGCG ATTCTGCATG ACAGGTGGGA ATAGCCCCAT CTGCTACCTC 
AAGGCCTACC ACACCAACCT CTACTTGTCA GCCGATGCAG ACAAAGTGCG AGAAGCCATC 
GATGAGGGGA TTGCAGCTGC CACCATCTTC AGCCCCAGCA GGGATGTGGT TGTGTCCCAG 
AGTCAGCTTC GAGTGGCCTT CGATGGGGAC GCAGTACTCT TCTCCGATGA GTCAGAGCGC 
ATCGTCAAGG CCCATGGGCT GGACAGATTC TTTGAGCATG AGAAGGCCCA CGAGAACAAA 
CCTTTGGCGC AGGGCCCCCT AAAGGGCTTC CTGGAAGCAC TGGGTAGACT GCAAAAGAAA 
TTCTACTCCA AGGGCCTGCG TCTGGAGTGC CCGATTCGCA CGTATTTGGT GACCGCGCGA 
AGTGCAGCCA GTTCCGGAGC CCGGGCCCTC AAGACCCTGC GCAGCTGGGG CCTGGAGACG 
GATGAGGCCT TATTCCTAGC TGGAGCACCC AAGGGTCCCC TTCTTGAGAA GATCCGTCCA 
CACATCTTCT TTGATGACCA GATGTTCCAC GTGGCTGGAG CTCAGGAGAT GGGCACGGTG 
GCCGCCCATG TGCCTTATGG TGTGGCTCAG AATCCCCGGC GGGCTGCCGC TGCGAAGCAG 
TCATTAGGTG CACAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nt5c1a ( NM_001085502.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001078971.1 Mus musculus 5'-nucleotidase, cytosolic IA (Nt5c1a), mRNA.

Price & Availability↑

CloneID	OMu02976
Clone ID Related Accession (Same CDS sequence)	NM_001085502.1 , NM_001085502.2
Accession Version	NM_001085502.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1098bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-17
Organism	Mus musculus(house mouse)
Product	cytosolic 5'-nucleotidase 1A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL606934.12. On Jun 18, 2019 this sequence version replaced NM_001085502.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR7345562.2322666.1, BC147730.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849379 [ECO:0000348] ##Evidence-Data-END##

ATGGAACCAG GGCAGCCCCG GGAGGCCCGC GAACCCGGGC CAGGGGCAGA GACCGCCGCG 
GTACCGCGCT GGGAGGAAGC CAAGACTTTC TATGACAACC TCTCGTCCAA GAAGAAACCC 
AAATCGCCAA AGCCTCAGAA TGCGGTCACC ATCGCGGTGT CCTCCAGAGC CTTGTTCCGA 
ATGGATGAGG AACAGAGGAT CTACACGGAG CAGGGTGTCG AGGAGTACGT GCGATACCAG 
CTGGAGCACG AGAATGAGCC CTTCAGCCCC GGGCCTGCCT TCCCCTTTGT GAAGGCCCTG 
GAGGCTGTGA ACAAGCGACT TCGGGAGTTG TACCCTGATA GCGAAGATAT CTTTGACATT 
GTCCTCATGA CCAACAACCA TGCTCAGGTG GGAGTTCGTC TCATCAACAG TATCAACCAT 
TATGACCTGT TCATTGAGCG ATTCTGCATG ACAGGTGGGA ATAGCCCCAT CTGCTACCTC 
AAGGCCTACC ACACCAACCT CTACTTGTCA GCCGATGCAG ACAAAGTGCG AGAAGCCATC 
GATGAGGGGA TTGCAGCTGC CACCATCTTC AGCCCCAGCA GGGATGTGGT TGTGTCCCAG 
AGTCAGCTTC GAGTGGCCTT CGATGGGGAC GCAGTACTCT TCTCCGATGA GTCAGAGCGC 
ATCGTCAAGG CCCATGGGCT GGACAGATTC TTTGAGCATG AGAAGGCCCA CGAGAACAAA 
CCTTTGGCGC AGGGCCCCCT AAAGGGCTTC CTGGAAGCAC TGGGTAGACT GCAAAAGAAA 
TTCTACTCCA AGGGCCTGCG TCTGGAGTGC CCGATTCGCA CGTATTTGGT GACCGCGCGA 
AGTGCAGCCA GTTCCGGAGC CCGGGCCCTC AAGACCCTGC GCAGCTGGGG CCTGGAGACG 
GATGAGGCCT TATTCCTAGC TGGAGCACCC AAGGGTCCCC TTCTTGAGAA GATCCGTCCA 
CACATCTTCT TTGATGACCA GATGTTCCAC GTGGCTGGAG CTCAGGAGAT GGGCACGGTG 
GCCGCCCATG TGCCTTATGG TGTGGCTCAG AATCCCCGGC GGGCTGCCGC TGCGAAGCAG 
TCATTAGGTG CACAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001078971.1
CDS	74..1171
Translation	MEPGQPREAREPGPGAETAAVPRWEEAKTFYDNLSSKKKPKSPKPQNAVTIAVSSRALFRMDEEQRIYTEQGVEEYVRYQLEHENEPFSPGPAFPFVKALEAVNKRLRELYPDSEDIFDIVLMTNNHAQVGVRLINSINHYDLFIERFCMTGGNSPICYLKAYHTNLYLSADADKVREAIDEGIAAATIFSPSRDVVVSQSQLRVAFDGDAVLFSDESERIVKAHGLDRFFEHEKAHENKPLAQGPLKGFLEALGRLQKKFYSKGLRLECPIRTYLVTARSAASSGARALKTLRSWGLETDEALFLAGAPKGPLLEKIRPHIFFDDQMFHVAGAQEMGTVAAHVPYGVAQNPRRAAAAKQSLGAQ

Target ORF information:

RefSeq Version	NM_001085502.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus 5'-nucleotidase, cytosolic IA (Nt5c1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001085502.2

ORF Insert Sequence:

ATGGAACCAG GGCAGCCCCG GGAGGCCCGC GAACCCGGGC CAGGGGCAGA GACCGCCGCG 
GTACCGCGCT GGGAGGAAGC CAAGACTTTC TATGACAACC TCTCGTCCAA GAAGAAACCC 
AAATCGCCAA AGCCTCAGAA TGCGGTCACC ATCGCGGTGT CCTCCAGAGC CTTGTTCCGA 
ATGGATGAGG AACAGAGGAT CTACACGGAG CAGGGTGTCG AGGAGTACGT GCGATACCAG 
CTGGAGCACG AGAATGAGCC CTTCAGCCCC GGGCCTGCCT TCCCCTTTGT GAAGGCCCTG 
GAGGCTGTGA ACAAGCGACT TCGGGAGTTG TACCCTGATA GCGAAGATAT CTTTGACATT 
GTCCTCATGA CCAACAACCA TGCTCAGGTG GGAGTTCGTC TCATCAACAG TATCAACCAT 
TATGACCTGT TCATTGAGCG ATTCTGCATG ACAGGTGGGA ATAGCCCCAT CTGCTACCTC 
AAGGCCTACC ACACCAACCT CTACTTGTCA GCCGATGCAG ACAAAGTGCG AGAAGCCATC 
GATGAGGGGA TTGCAGCTGC CACCATCTTC AGCCCCAGCA GGGATGTGGT TGTGTCCCAG 
AGTCAGCTTC GAGTGGCCTT CGATGGGGAC GCAGTACTCT TCTCCGATGA GTCAGAGCGC 
ATCGTCAAGG CCCATGGGCT GGACAGATTC TTTGAGCATG AGAAGGCCCA CGAGAACAAA 
CCTTTGGCGC AGGGCCCCCT AAAGGGCTTC CTGGAAGCAC TGGGTAGACT GCAAAAGAAA 
TTCTACTCCA AGGGCCTGCG TCTGGAGTGC CCGATTCGCA CGTATTTGGT GACCGCGCGA 
AGTGCAGCCA GTTCCGGAGC CCGGGCCCTC AAGACCCTGC GCAGCTGGGG CCTGGAGACG 
GATGAGGCCT TATTCCTAGC TGGAGCACCC AAGGGTCCCC TTCTTGAGAA GATCCGTCCA 
CACATCTTCT TTGATGACCA GATGTTCCAC GTGGCTGGAG CTCAGGAGAT GGGCACGGTG 
GCCGCCCATG TGCCTTATGG TGTGGCTCAG AATCCCCGGC GGGCTGCCGC TGCGAAGCAG 
TCATTAGGTG CACAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Effects of genetic deletion of soluble 5'-nucleotidases NT5C1A and NT5C2 on AMPK activation and nucleotide levels in contracting mouse skeletal muscles.
American journal of physiology. Endocrinology and metabolism313(1)E48-E62(2017 Jul)
Kviklyte S,Vertommen D,Yerna X,Anders?n H,Xu X,Gailly P,Bohlooly-Y M,Oscarsson J,Rider MH

Suppression of 5'-nucleotidase enzymes promotes AMP-activated protein kinase (AMPK) phosphorylation and metabolism in human and mouse skeletal muscle.
The Journal of biological chemistry286(40)34567-74(2011 Oct)
Kulkarni SS,Karlsson HK,Szekeres F,Chibalin AV,Krook A,Zierath JR

Gene structure and evolution of testicular haploid germ cell-specific genes, Oxct2a and Oxct2b.
Genomics83(4)647-57(2004 Apr)
Onishi M,Yasunaga T,Tanaka H,Nishimune Y,Nozaki M

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Nt5c1a cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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