Prdm13 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prdm13
Entrez Gene ID	230025
Full Name	PR domain containing 13
General protein information	Preferred Names PR domain containing 13 Names PR domain zinc finger protein 13 PR domain-containing protein 13
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 A3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006537807.2	XP_006537870.1	PR domain zinc finger protein 13 isoform X1
XM_006537806.2	XP_006537869.1	PR domain zinc finger protein 13 isoform X1
NM_001080771.1	NP_001074240.1	PR domain zinc finger protein 13

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Prdm13	NP_001074240.1
Danio rerio (zebrafish)	prdm13	XP_001336591.2
Rattus norvegicus (Norway rat)	Prdm13	XP_006238009.1
Xenopus tropicalis (tropical clawed frog)	prdm13	XP_002932206.1
Homo sapiens (human)	PRDM13	NP_067633.2
Pan troglodytes (chimpanzee)	PRDM13	XP_003311465.1
	LOC100428049	XP_002803888.1
Bos taurus (cattle)	PRDM13	NP_001179939.1

Gene Ontology
Bibliography

Related articles in PubMed

Prdm13 is required for Ebf3+ amacrine cell formation in the retina.
Goodson NB, Nahreini J, Randazzo G, Uruena A, Johnson JE, Brzezinski JA 4th
Developmental biology(2017 Dec)

Repression by PRDM13 is critical for generating precision in neuronal identity.
Mona B, Uruena A, Kollipara RK, Ma Z, Borromeo MD, Chang JC, Johnson JE
eLife6(2017 Aug)

Prdm13 regulates subtype specification of retinal amacrine interneurons and modulates visual sensitivity.
Watanabe S, Sanuki R, Sugita Y, Imai W, Yamazaki R, Kozuka T, Ohsuga M, Furukawa T
The Journal of neuroscience : the official journal of the Society for Neuroscience35(20)8004-20(2015 May)

Deficiency of Prdm13, a dorsomedial hypothalamus-enriched gene, mimics age-associated changes in sleep quality and adiposity.
Satoh A, Brace CS, Rensing N, Imai S
Aging cell14(2)209-18(2015 Apr)

The Prdm13 histone methyltransferase encoding gene is a Ptf1a-Rbpj downstream target that suppresses glutamatergic and promotes GABAergic neuronal fate in the dorsal neural tube.
Hanotel J, Bessodes N, Th?lie A, Hedderich M, Parain K, Van Driessche B, Brand?o Kde O, Kricha S, Jorgensen MC, Grapin-Botton A, Serup P, Van Lint C, Perron M, Pieler T, Henningfeld KA, Bellefroid EJ
Developmental biology386(2)340-57(2014 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Targeted deletion of Prdm13 leads to a specific reduction of amacrine cells and changes visual sensitivity.
Title: Prdm13 regulates subtype specification of retinal amacrine interneurons and modulates visual sensitivity.

Findings indicate that Prdm13/Nkx2-1-mediated signaling in the DMC declines with advanced age, leading to decreased sleep quality and increased adiposity.
Title: Deficiency of Prdm13, a dorsomedial hypothalamus-enriched gene, mimics age-associated changes in sleep quality and adiposity.

The following Prdm13 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prdm13 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu01440	NM_001080771.1 Latest version!	Mus musculus PR domain containing 13 (Prdm13), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$447.30 $639.00
OMu32865	XM_006537806.2 Latest version!	Mus musculus PR domain containing 13 (Prdm13), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
OMu32865	XM_006537807.2 Latest version!	Mus musculus PR domain containing 13 (Prdm13), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prdm13 ( NM_001080771.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001074240.1 Mus musculus PR domain containing 13 (Prdm13), mRNA.

Price & Availability↑

CloneID	OMu01440
Clone ID Related Accession (Same CDS sequence)	NM_001080771.1
Accession Version	NM_001080771.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2265bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	PR domain zinc finger protein 13
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC017632.1 and BB665287.1. On or before Jan 26, 2007 this sequence version replaced XM_907402.2, XM_920394.2, XM_884367.2, XM_131309.6, XM_977119.1, XM_977082.1. ##Evidence-Data-START## Transcript exon combination :: BB665287.1, BY731300.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164138, SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCGCCC ACGTCACTCC GCGGACAGAA GACGCACGTC GGGGTGCGGG TCCCTCCTCT 
GCATGCGGCT GCTCCTGGTT CTGTCACTTG CGCCCCGTGG AGGACCCCGC GAGCCCCAGC 
GTCTGCCTGG CGGCGGTGGC AACAATGCAC GGAACTTCTC GCACTTCGGC CACCAGCGTG 
AACGCTGACT GCTGCATCCC GGCTGGCTTG CGCCTTGGGC CGGTGCCTGG CACCTTCAAG 
CTGGGCAAGT ACCTGTCAGA TCGCAGGGAA CCAGGGCCCA AGAAAAAGGT GCGCATGGTG 
AGAGGGGAGC TGGTGGACGA GTCAGGGGGC TCCCCTCTGG AGTGGATAGG GTTAATCCGG 
GCAGCCAGGA ATCCCCAGGA ACAGACTCTG GAAGCTATTG CAGACTTACC AGGAGGACAG 
ATCTTCTATC GGGCATTGCG AGACGTCCAG CCTGGGGAAG AGCTGACTGT GTGGTATTCT 
AACTCCTTGG CTCAGTGGTT TGACATCCCT ACAACTGCGA CTCCAACGCA TGATGAGAAA 
GGGGAGGAGC GCTACATCTG CTGGTACTGC TGGAGGACCT TTCGCTACCC TAACAGCCTC 
AAGGCACACC TGCGGTTCCA CTGCGTCCTC AGTGGCGGCG GGGGCCGTGC TTTCCTGCCC 
CAGGAGCACG CGGCCCGGCC CGGCGCTTCC CCGGTGGCAG AAGGCCTCGG CCTCCCTCCG 
AAACCCACCG TCCCGGACTT GACGGCGCCC GTCCAGGCAA TCGCTTTGCG ACCCCAAGCT 
CCGGCCGCTC AGCTCGCCCA GGCGTGCGGG GCGCGGGAGA GCATCAAGCG GGAGGCTTCC 
CTGGCGCCCC TGGCCACCTC CCCGCCGCCG GGCAAGTGGG GAACGCCCAA GAAGGGCAAG 
GAGCAGCCGG ACCGCGCGCA CAGTCAATTC CTGGGGATAG TGGGCGGCTC CTCGGGCGGC 
GGCGGAGGCC TGCCATTCTA CCCAGGCGTG CGCTCGGCTT TCAAGCCGGC CGGCCTGGCC 
CGGGCGGCTG CGCAGAGCGA CCCCTACCGG GAAGAGGGCG GCGGCAAGGG ACCGGGGCTG 
GCGCTAGGGC GGCTGCTGGG CGGGGGCCGG GCCGGCGGGC GTCCGGGCAG TGGGGAGAGC 
CCGGCGGGCC ACCACCACCA TCACCACCAC GCTCACCACC ACCACCACCA CCATCCCAAG 
TGCCTGCTGG CCGGGGAGCC GCCGCCGGCG GGCCTGCCGT GTCCCGGGGC CCTGAGAGCC 
TTCCCCCTGC TCGCCGGCCA CCCGGAAGAG GCGTCGGCCT TCAAGCACGT GGAGCGGGCT 
CCGCCCGCCG CCGCCACCAC CTCCCTGCCC TCCGCGCGCT ACGCCGCTCT CCCGGCGCCC 
GGGCTCCCTG TGGAGCGCTG CGCGCTGCAG CCCCTCGACG GCGGCAGCCT CAAGGCTTAC 
CCCGGCGGCG GTGGCGGCGG CGAGTGCAGC CCCCTGCCCG CCGTCATGCC GGCCTTCACG 
GTGTACAGCG GAGACCTGCT CTACGGCCCT CCGGCCGCCT ATTACCCTCT CAAGCTGCAC 
TTGGGCGGGC TGCTCAAATA CCCCGAGTCC ATCTCCTATC TGAGTGGCCC CGCGGCGGCA 
GCGGCGGCGG CGGCAGCGGC AGCGGCGGCG GCGGCGGCGA TCGGGCCGGC CGAACTGGGC 
TCCCTGGCCA GCATCGACCG CGAGATCGCC ATGCACACAC AGCAGCTGTC CGAGATGGCA 
GCCGGGAAGA GTCGCGCCCG CCTGGACTCG GGGACGCTGC CTCCAGCCGT AGTGGCGGCG 
ACGGGCCCCG GGGGTGGCGG CGGTGGCGGG AGCGCGGCAG GCAAGCCCAA GACAGGCCAC 
TTGTGCCTCT ACTGTGGCAA GCTGTACTCG CGCAAGTACG GGCTCAAGAT CCACATGCGG 
ACGCACACGG GCTACAAGCC GCTCAAGTGC AAAGTGTGCC TGCGGCCCTT CGGCGACCCC 
AGCAATCTCA ACAAGCACAT CCGACTCCAC GCCGAGGGCA ATACGCCCTA CCGCTGCGAG 
TTCTGCGGCA AGGTGCTGGT GCGCCGCCGA GACCTGGAGC GTCACGTCAA GTCCCGCCAC 
CCAGGCCAGA GCCTGATGGC CAAGGCGGGC GACGGCCCGG GCCCGGAGCC CAGCTATGCT 
CTGGAGCCTG GGGATCCCAA GAGCGAGGAC AGTGATGTGG ATGTCTGCTT CACTGACGAT 
CAGAGCGACC CCGAGGCTGG GGGTCGTGGC GAGCACGACT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074240.1
CDS	166..2430
Translation	MPAHVTPRTEDARRGAGPSSACGCSWFCHLRPVEDPASPSVCLAAVATMHGTSRTSATSVNADCCIPAGLRLGPVPGTFKLGKYLSDRREPGPKKKVRMVRGELVDESGGSPLEWIGLIRAARNPQEQTLEAIADLPGGQIFYRALRDVQPGEELTVWYSNSLAQWFDIPTTATPTHDEKGEERYICWYCWRTFRYPNSLKAHLRFHCVLSGGGGRAFLPQEHAARPGASPVAEGLGLPPKPTVPDLTAPVQAIALRPQAPAAQLAQACGARESIKREASLAPLATSPPPGKWGTPKKGKEQPDRAHSQFLGIVGGSSGGGGGLPFYPGVRSAFKPAGLARAAAQSDPYREEGGGKGPGLALGRLLGGGRAGGRPGSGESPAGHHHHHHHAHHHHHHHPKCLLAGEPPPAGLPCPGALRAFPLLAGHPEEASAFKHVERAPPAAATTSLPSARYAALPAPGLPVERCALQPLDGGSLKAYPGGGGGGECSPLPAVMPAFTVYSGDLLYGPPAAYYPLKLHLGGLLKYPESISYLSGPAAAAAAAAAAAAAAAAIGPAELGSLASIDREIAMHTQQLSEMAAGKSRARLDSGTLPPAVVAATGPGGGGGGGSAAGKPKTGHLCLYCGKLYSRKYGLKIHMRTHTGYKPLKCKVCLRPFGDPSNLNKHIRLHAEGNTPYRCEFCGKVLVRRRDLERHVKSRHPGQSLMAKAGDGPGPEPSYALEPGDPKSEDSDVDVCFTDDQSDPEAGGRGEHDS

Target ORF information:

RefSeq Version	NM_001080771.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus PR domain containing 13 (Prdm13), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001080771.1

ORF Insert Sequence:

ATGCCCGCCC ACGTCACTCC GCGGACAGAA GACGCACGTC GGGGTGCGGG TCCCTCCTCT 
GCATGCGGCT GCTCCTGGTT CTGTCACTTG CGCCCCGTGG AGGACCCCGC GAGCCCCAGC 
GTCTGCCTGG CGGCGGTGGC AACAATGCAC GGAACTTCTC GCACTTCGGC CACCAGCGTG 
AACGCTGACT GCTGCATCCC GGCTGGCTTG CGCCTTGGGC CGGTGCCTGG CACCTTCAAG 
CTGGGCAAGT ACCTGTCAGA TCGCAGGGAA CCAGGGCCCA AGAAAAAGGT GCGCATGGTG 
AGAGGGGAGC TGGTGGACGA GTCAGGGGGC TCCCCTCTGG AGTGGATAGG GTTAATCCGG 
GCAGCCAGGA ATCCCCAGGA ACAGACTCTG GAAGCTATTG CAGACTTACC AGGAGGACAG 
ATCTTCTATC GGGCATTGCG AGACGTCCAG CCTGGGGAAG AGCTGACTGT GTGGTATTCT 
AACTCCTTGG CTCAGTGGTT TGACATCCCT ACAACTGCGA CTCCAACGCA TGATGAGAAA 
GGGGAGGAGC GCTACATCTG CTGGTACTGC TGGAGGACCT TTCGCTACCC TAACAGCCTC 
AAGGCACACC TGCGGTTCCA CTGCGTCCTC AGTGGCGGCG GGGGCCGTGC TTTCCTGCCC 
CAGGAGCACG CGGCCCGGCC CGGCGCTTCC CCGGTGGCAG AAGGCCTCGG CCTCCCTCCG 
AAACCCACCG TCCCGGACTT GACGGCGCCC GTCCAGGCAA TCGCTTTGCG ACCCCAAGCT 
CCGGCCGCTC AGCTCGCCCA GGCGTGCGGG GCGCGGGAGA GCATCAAGCG GGAGGCTTCC 
CTGGCGCCCC TGGCCACCTC CCCGCCGCCG GGCAAGTGGG GAACGCCCAA GAAGGGCAAG 
GAGCAGCCGG ACCGCGCGCA CAGTCAATTC CTGGGGATAG TGGGCGGCTC CTCGGGCGGC 
GGCGGAGGCC TGCCATTCTA CCCAGGCGTG CGCTCGGCTT TCAAGCCGGC CGGCCTGGCC 
CGGGCGGCTG CGCAGAGCGA CCCCTACCGG GAAGAGGGCG GCGGCAAGGG ACCGGGGCTG 
GCGCTAGGGC GGCTGCTGGG CGGGGGCCGG GCCGGCGGGC GTCCGGGCAG TGGGGAGAGC 
CCGGCGGGCC ACCACCACCA TCACCACCAC GCTCACCACC ACCACCACCA CCATCCCAAG 
TGCCTGCTGG CCGGGGAGCC GCCGCCGGCG GGCCTGCCGT GTCCCGGGGC CCTGAGAGCC 
TTCCCCCTGC TCGCCGGCCA CCCGGAAGAG GCGTCGGCCT TCAAGCACGT GGAGCGGGCT 
CCGCCCGCCG CCGCCACCAC CTCCCTGCCC TCCGCGCGCT ACGCCGCTCT CCCGGCGCCC 
GGGCTCCCTG TGGAGCGCTG CGCGCTGCAG CCCCTCGACG GCGGCAGCCT CAAGGCTTAC 
CCCGGCGGCG GTGGCGGCGG CGAGTGCAGC CCCCTGCCCG CCGTCATGCC GGCCTTCACG 
GTGTACAGCG GAGACCTGCT CTACGGCCCT CCGGCCGCCT ATTACCCTCT CAAGCTGCAC 
TTGGGCGGGC TGCTCAAATA CCCCGAGTCC ATCTCCTATC TGAGTGGCCC CGCGGCGGCA 
GCGGCGGCGG CGGCAGCGGC AGCGGCGGCG GCGGCGGCGA TCGGGCCGGC CGAACTGGGC 
TCCCTGGCCA GCATCGACCG CGAGATCGCC ATGCACACAC AGCAGCTGTC CGAGATGGCA 
GCCGGGAAGA GTCGCGCCCG CCTGGACTCG GGGACGCTGC CTCCAGCCGT AGTGGCGGCG 
ACGGGCCCCG GGGGTGGCGG CGGTGGCGGG AGCGCGGCAG GCAAGCCCAA GACAGGCCAC 
TTGTGCCTCT ACTGTGGCAA GCTGTACTCG CGCAAGTACG GGCTCAAGAT CCACATGCGG 
ACGCACACGG GCTACAAGCC GCTCAAGTGC AAAGTGTGCC TGCGGCCCTT CGGCGACCCC 
AGCAATCTCA ACAAGCACAT CCGACTCCAC GCCGAGGGCA ATACGCCCTA CCGCTGCGAG 
TTCTGCGGCA AGGTGCTGGT GCGCCGCCGA GACCTGGAGC GTCACGTCAA GTCCCGCCAC 
CCAGGCCAGA GCCTGATGGC CAAGGCGGGC GACGGCCCGG GCCCGGAGCC CAGCTATGCT 
CTGGAGCCTG GGGATCCCAA GAGCGAGGAC AGTGATGTGG ATGTCTGCTT CACTGACGAT 
CAGAGCGACC CCGAGGCTGG GGGTCGTGGC GAGCACGACT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prdm13 ( XM_006537807.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006537870.1 Mus musculus PR domain containing 13 (Prdm13), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu32865
Clone ID Related Accession (Same CDS sequence)	XM_006537807.2 , XM_006537806.2
Accession Version	XM_006537807.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	PR domain zinc finger protein 13 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006537807.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAGAG GGGAGCTGGT GGACGAGTCA GGGGGCTCCC CTCTGGAGTG GATAGGGTTA 
ATCCGGGCAG CCAGGAATCC CCAGGAACAG ACTCTGGAAG CTATTGCAGA CTTACCAGGA 
GGACAGATCT TCTATCGGGC ATTGCGAGAC GTCCAGCCTG GGGAAGAGCT GACTGTGTGG 
TATTCTAACT CCTTGGCTCA GTGGTTTGAC ATCCCTACAA CTGCGACTCC AACGCATGAT 
GAGAAAGGGG AGGAGCGCTA CATCTGCTGG TACTGCTGGA GGACCTTTCG CTACCCTAAC 
AGCCTCAAGG CACACCTGCG GTTCCACTGC GTCCTCAGTG GCGGCGGGGG CCGTGCTTTC 
CTGCCCCAGG AGCACGCGGC CCGGCCCGGC GCTTCCCCGG TGGCAGAAGG CCTCGGCCTC 
CCTCCGAAAC CCACCGTCCC GGACTTGACG GCGCCCGTCC AGGCAATCGC TTTGCGACCC 
CAAGCTCCGG CCGCTCAGCT CGCCCAGGCG TGCGGGGCGC GGGAGAGCAT CAAGCGGGAG 
GCTTCCCTGG CGCCCCTGGC CACCTCCCCG CCGCCGGGCA AGTGGGGAAC GCCCAAGAAG 
GGCAAGGAGC AGCCGGACCG CGCGCACAGT CAATTCCTGG GGATAGTGGG CGGCTCCTCG 
GGCGGCGGCG GAGGCCTGCC ATTCTACCCA GGCGTGCGCT CGGCTTTCAA GCCGGCCGGC 
CTGGCCCGGG CGGCTGCGCA GAGCGACCCC TACCGGGAAG AGGGCGGCGG CAAGGGACCG 
GGGCTGGCGC TAGGGCGGCT GCTGGGCGGG GGCCGGGCCG GCGGGCGTCC GGGCAGTGGG 
GAGAGCCCGG CGGGCCACCA CCACCATCAC CACCACGCTC ACCACCACCA CCACCACCAT 
CCCAAGTGCC TGCTGGCCGG GGAGCCGCCG CCGGCGGGCC TGCCGTGTCC CGGGGCCCTG 
AGAGCCTTCC CCCTGCTCGC CGGCCACCCG GAAGAGGCGT CGGCCTTCAA GCACGTGGAG 
CGGGCTCCGC CCGCCGCCGC CACCACCTCC CTGCCCTCCG CGCGCTACGC CGCTCTCCCG 
GCGCCCGGGC TCCCTGTGGA GCGCTGCGCG CTGCAGCCCC TCGACGGCGG CAGCCTCAAG 
GCTTACCCCG GCGGCGGTGG CGGCGGCGAG TGCAGCCCCC TGCCCGCCGT CATGCCGGCC 
TTCACGGTGT ACAGCGGAGA CCTGCTCTAC GGCCCTCCGG CCGCCTATTA CCCTCTCAAG 
CTGCACTTGG GCGGGCTGCT CAAATACCCC GAGTCCATCT CCTATCTGAG TGGCCCCGCG 
GCGGCAGCGG CGGCGGCGGC AGCGGCAGCG GCGGCGGCGG CGGCGATCGG GCCGGCCGAA 
CTGGGCTCCC TGGCCAGCAT CGACCGCGAG ATCGCCATGC ACACACAGCA GCTGTCCGAG 
ATGGCAGCCG GGAAGAGTCG CGCCCGCCTG GACTCGGGGA CGCTGCCTCC AGCCGTAGTG 
GCGGCGACGG GCCCCGGGGG TGGCGGCGGT GGCGGGAGCG CGGCAGGCAA GCCCAAGACA 
GGCCACTTGT GCCTCTACTG TGGCAAGCTG TACTCGCGCA AGTACGGGCT CAAGATCCAC 
ATGCGGACGC ACACGGGCTA CAAGCCGCTC AAGTGCAAAG TGTGCCTGCG GCCCTTCGGC 
GACCCCAGCA ATCTCAACAA GCACATCCGA CTCCACGCCG AGGGCAATAC GCCCTACCGC 
TGCGAGTTCT GCGGCAAGGT GCTGGTGCGC CGCCGAGACC TGGAGCGTCA CGTCAAGTCC 
CGCCACCCAG GCCAGAGCCT GATGGCCAAG GCGGGCGACG GCCCGGGCCC GGAGCCCAGC 
TATGCTCTGG AGCCTGGGGA TCCCAAGAGC GAGGACAGTG ATGTGGATGT CTGCTTCACT 
GACGATCAGA GCGACCCCGA GGCTGGGGGT CGTGGCGAGC ACGACTCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006537870.1
CDS	1189..3159
Translation	MVRGELVDESGGSPLEWIGLIRAARNPQEQTLEAIADLPGGQIFYRALRDVQPGEELTVWYSNSLAQWFDIPTTATPTHDEKGEERYICWYCWRTFRYPNSLKAHLRFHCVLSGGGGRAFLPQEHAARPGASPVAEGLGLPPKPTVPDLTAPVQAIALRPQAPAAQLAQACGARESIKREASLAPLATSPPPGKWGTPKKGKEQPDRAHSQFLGIVGGSSGGGGGLPFYPGVRSAFKPAGLARAAAQSDPYREEGGGKGPGLALGRLLGGGRAGGRPGSGESPAGHHHHHHHAHHHHHHHPKCLLAGEPPPAGLPCPGALRAFPLLAGHPEEASAFKHVERAPPAAATTSLPSARYAALPAPGLPVERCALQPLDGGSLKAYPGGGGGGECSPLPAVMPAFTVYSGDLLYGPPAAYYPLKLHLGGLLKYPESISYLSGPAAAAAAAAAAAAAAAAIGPAELGSLASIDREIAMHTQQLSEMAAGKSRARLDSGTLPPAVVAATGPGGGGGGGSAAGKPKTGHLCLYCGKLYSRKYGLKIHMRTHTGYKPLKCKVCLRPFGDPSNLNKHIRLHAEGNTPYRCEFCGKVLVRRRDLERHVKSRHPGQSLMAKAGDGPGPEPSYALEPGDPKSEDSDVDVCFTDDQSDPEAGGRGEHDS

Target ORF information:

RefSeq Version	XM_006537807.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus PR domain containing 13 (Prdm13), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006537807.2

ORF Insert Sequence:

ATGGTGAGAG GGGAGCTGGT GGACGAGTCA GGGGGCTCCC CTCTGGAGTG GATAGGGTTA 
ATCCGGGCAG CCAGGAATCC CCAGGAACAG ACTCTGGAAG CTATTGCAGA CTTACCAGGA 
GGACAGATCT TCTATCGGGC ATTGCGAGAC GTCCAGCCTG GGGAAGAGCT GACTGTGTGG 
TATTCTAACT CCTTGGCTCA GTGGTTTGAC ATCCCTACAA CTGCGACTCC AACGCATGAT 
GAGAAAGGGG AGGAGCGCTA CATCTGCTGG TACTGCTGGA GGACCTTTCG CTACCCTAAC 
AGCCTCAAGG CACACCTGCG GTTCCACTGC GTCCTCAGTG GCGGCGGGGG CCGTGCTTTC 
CTGCCCCAGG AGCACGCGGC CCGGCCCGGC GCTTCCCCGG TGGCAGAAGG CCTCGGCCTC 
CCTCCGAAAC CCACCGTCCC GGACTTGACG GCGCCCGTCC AGGCAATCGC TTTGCGACCC 
CAAGCTCCGG CCGCTCAGCT CGCCCAGGCG TGCGGGGCGC GGGAGAGCAT CAAGCGGGAG 
GCTTCCCTGG CGCCCCTGGC CACCTCCCCG CCGCCGGGCA AGTGGGGAAC GCCCAAGAAG 
GGCAAGGAGC AGCCGGACCG CGCGCACAGT CAATTCCTGG GGATAGTGGG CGGCTCCTCG 
GGCGGCGGCG GAGGCCTGCC ATTCTACCCA GGCGTGCGCT CGGCTTTCAA GCCGGCCGGC 
CTGGCCCGGG CGGCTGCGCA GAGCGACCCC TACCGGGAAG AGGGCGGCGG CAAGGGACCG 
GGGCTGGCGC TAGGGCGGCT GCTGGGCGGG GGCCGGGCCG GCGGGCGTCC GGGCAGTGGG 
GAGAGCCCGG CGGGCCACCA CCACCATCAC CACCACGCTC ACCACCACCA CCACCACCAT 
CCCAAGTGCC TGCTGGCCGG GGAGCCGCCG CCGGCGGGCC TGCCGTGTCC CGGGGCCCTG 
AGAGCCTTCC CCCTGCTCGC CGGCCACCCG GAAGAGGCGT CGGCCTTCAA GCACGTGGAG 
CGGGCTCCGC CCGCCGCCGC CACCACCTCC CTGCCCTCCG CGCGCTACGC CGCTCTCCCG 
GCGCCCGGGC TCCCTGTGGA GCGCTGCGCG CTGCAGCCCC TCGACGGCGG CAGCCTCAAG 
GCTTACCCCG GCGGCGGTGG CGGCGGCGAG TGCAGCCCCC TGCCCGCCGT CATGCCGGCC 
TTCACGGTGT ACAGCGGAGA CCTGCTCTAC GGCCCTCCGG CCGCCTATTA CCCTCTCAAG 
CTGCACTTGG GCGGGCTGCT CAAATACCCC GAGTCCATCT CCTATCTGAG TGGCCCCGCG 
GCGGCAGCGG CGGCGGCGGC AGCGGCAGCG GCGGCGGCGG CGGCGATCGG GCCGGCCGAA 
CTGGGCTCCC TGGCCAGCAT CGACCGCGAG ATCGCCATGC ACACACAGCA GCTGTCCGAG 
ATGGCAGCCG GGAAGAGTCG CGCCCGCCTG GACTCGGGGA CGCTGCCTCC AGCCGTAGTG 
GCGGCGACGG GCCCCGGGGG TGGCGGCGGT GGCGGGAGCG CGGCAGGCAA GCCCAAGACA 
GGCCACTTGT GCCTCTACTG TGGCAAGCTG TACTCGCGCA AGTACGGGCT CAAGATCCAC 
ATGCGGACGC ACACGGGCTA CAAGCCGCTC AAGTGCAAAG TGTGCCTGCG GCCCTTCGGC 
GACCCCAGCA ATCTCAACAA GCACATCCGA CTCCACGCCG AGGGCAATAC GCCCTACCGC 
TGCGAGTTCT GCGGCAAGGT GCTGGTGCGC CGCCGAGACC TGGAGCGTCA CGTCAAGTCC 
CGCCACCCAG GCCAGAGCCT GATGGCCAAG GCGGGCGACG GCCCGGGCCC GGAGCCCAGC 
TATGCTCTGG AGCCTGGGGA TCCCAAGAGC GAGGACAGTG ATGTGGATGT CTGCTTCACT 
GACGATCAGA GCGACCCCGA GGCTGGGGGT CGTGGCGAGC ACGACTCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prdm13 ( XM_006537806.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006537869.1 Mus musculus PR domain containing 13 (Prdm13), transcript variant X1, mRNA.

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CloneID	OMu32865
Clone ID Related Accession (Same CDS sequence)	XM_006537807.2 , XM_006537806.2
Accession Version	XM_006537806.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	PR domain zinc finger protein 13 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006537806.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAGAG GGGAGCTGGT GGACGAGTCA GGGGGCTCCC CTCTGGAGTG GATAGGGTTA 
ATCCGGGCAG CCAGGAATCC CCAGGAACAG ACTCTGGAAG CTATTGCAGA CTTACCAGGA 
GGACAGATCT TCTATCGGGC ATTGCGAGAC GTCCAGCCTG GGGAAGAGCT GACTGTGTGG 
TATTCTAACT CCTTGGCTCA GTGGTTTGAC ATCCCTACAA CTGCGACTCC AACGCATGAT 
GAGAAAGGGG AGGAGCGCTA CATCTGCTGG TACTGCTGGA GGACCTTTCG CTACCCTAAC 
AGCCTCAAGG CACACCTGCG GTTCCACTGC GTCCTCAGTG GCGGCGGGGG CCGTGCTTTC 
CTGCCCCAGG AGCACGCGGC CCGGCCCGGC GCTTCCCCGG TGGCAGAAGG CCTCGGCCTC 
CCTCCGAAAC CCACCGTCCC GGACTTGACG GCGCCCGTCC AGGCAATCGC TTTGCGACCC 
CAAGCTCCGG CCGCTCAGCT CGCCCAGGCG TGCGGGGCGC GGGAGAGCAT CAAGCGGGAG 
GCTTCCCTGG CGCCCCTGGC CACCTCCCCG CCGCCGGGCA AGTGGGGAAC GCCCAAGAAG 
GGCAAGGAGC AGCCGGACCG CGCGCACAGT CAATTCCTGG GGATAGTGGG CGGCTCCTCG 
GGCGGCGGCG GAGGCCTGCC ATTCTACCCA GGCGTGCGCT CGGCTTTCAA GCCGGCCGGC 
CTGGCCCGGG CGGCTGCGCA GAGCGACCCC TACCGGGAAG AGGGCGGCGG CAAGGGACCG 
GGGCTGGCGC TAGGGCGGCT GCTGGGCGGG GGCCGGGCCG GCGGGCGTCC GGGCAGTGGG 
GAGAGCCCGG CGGGCCACCA CCACCATCAC CACCACGCTC ACCACCACCA CCACCACCAT 
CCCAAGTGCC TGCTGGCCGG GGAGCCGCCG CCGGCGGGCC TGCCGTGTCC CGGGGCCCTG 
AGAGCCTTCC CCCTGCTCGC CGGCCACCCG GAAGAGGCGT CGGCCTTCAA GCACGTGGAG 
CGGGCTCCGC CCGCCGCCGC CACCACCTCC CTGCCCTCCG CGCGCTACGC CGCTCTCCCG 
GCGCCCGGGC TCCCTGTGGA GCGCTGCGCG CTGCAGCCCC TCGACGGCGG CAGCCTCAAG 
GCTTACCCCG GCGGCGGTGG CGGCGGCGAG TGCAGCCCCC TGCCCGCCGT CATGCCGGCC 
TTCACGGTGT ACAGCGGAGA CCTGCTCTAC GGCCCTCCGG CCGCCTATTA CCCTCTCAAG 
CTGCACTTGG GCGGGCTGCT CAAATACCCC GAGTCCATCT CCTATCTGAG TGGCCCCGCG 
GCGGCAGCGG CGGCGGCGGC AGCGGCAGCG GCGGCGGCGG CGGCGATCGG GCCGGCCGAA 
CTGGGCTCCC TGGCCAGCAT CGACCGCGAG ATCGCCATGC ACACACAGCA GCTGTCCGAG 
ATGGCAGCCG GGAAGAGTCG CGCCCGCCTG GACTCGGGGA CGCTGCCTCC AGCCGTAGTG 
GCGGCGACGG GCCCCGGGGG TGGCGGCGGT GGCGGGAGCG CGGCAGGCAA GCCCAAGACA 
GGCCACTTGT GCCTCTACTG TGGCAAGCTG TACTCGCGCA AGTACGGGCT CAAGATCCAC 
ATGCGGACGC ACACGGGCTA CAAGCCGCTC AAGTGCAAAG TGTGCCTGCG GCCCTTCGGC 
GACCCCAGCA ATCTCAACAA GCACATCCGA CTCCACGCCG AGGGCAATAC GCCCTACCGC 
TGCGAGTTCT GCGGCAAGGT GCTGGTGCGC CGCCGAGACC TGGAGCGTCA CGTCAAGTCC 
CGCCACCCAG GCCAGAGCCT GATGGCCAAG GCGGGCGACG GCCCGGGCCC GGAGCCCAGC 
TATGCTCTGG AGCCTGGGGA TCCCAAGAGC GAGGACAGTG ATGTGGATGT CTGCTTCACT 
GACGATCAGA GCGACCCCGA GGCTGGGGGT CGTGGCGAGC ACGACTCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006537869.1
CDS	1256..3226
Translation	MVRGELVDESGGSPLEWIGLIRAARNPQEQTLEAIADLPGGQIFYRALRDVQPGEELTVWYSNSLAQWFDIPTTATPTHDEKGEERYICWYCWRTFRYPNSLKAHLRFHCVLSGGGGRAFLPQEHAARPGASPVAEGLGLPPKPTVPDLTAPVQAIALRPQAPAAQLAQACGARESIKREASLAPLATSPPPGKWGTPKKGKEQPDRAHSQFLGIVGGSSGGGGGLPFYPGVRSAFKPAGLARAAAQSDPYREEGGGKGPGLALGRLLGGGRAGGRPGSGESPAGHHHHHHHAHHHHHHHPKCLLAGEPPPAGLPCPGALRAFPLLAGHPEEASAFKHVERAPPAAATTSLPSARYAALPAPGLPVERCALQPLDGGSLKAYPGGGGGGECSPLPAVMPAFTVYSGDLLYGPPAAYYPLKLHLGGLLKYPESISYLSGPAAAAAAAAAAAAAAAAIGPAELGSLASIDREIAMHTQQLSEMAAGKSRARLDSGTLPPAVVAATGPGGGGGGGSAAGKPKTGHLCLYCGKLYSRKYGLKIHMRTHTGYKPLKCKVCLRPFGDPSNLNKHIRLHAEGNTPYRCEFCGKVLVRRRDLERHVKSRHPGQSLMAKAGDGPGPEPSYALEPGDPKSEDSDVDVCFTDDQSDPEAGGRGEHDS

Target ORF information:

RefSeq Version	XM_006537806.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus PR domain containing 13 (Prdm13), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006537806.2

ORF Insert Sequence:

ATGGTGAGAG GGGAGCTGGT GGACGAGTCA GGGGGCTCCC CTCTGGAGTG GATAGGGTTA 
ATCCGGGCAG CCAGGAATCC CCAGGAACAG ACTCTGGAAG CTATTGCAGA CTTACCAGGA 
GGACAGATCT TCTATCGGGC ATTGCGAGAC GTCCAGCCTG GGGAAGAGCT GACTGTGTGG 
TATTCTAACT CCTTGGCTCA GTGGTTTGAC ATCCCTACAA CTGCGACTCC AACGCATGAT 
GAGAAAGGGG AGGAGCGCTA CATCTGCTGG TACTGCTGGA GGACCTTTCG CTACCCTAAC 
AGCCTCAAGG CACACCTGCG GTTCCACTGC GTCCTCAGTG GCGGCGGGGG CCGTGCTTTC 
CTGCCCCAGG AGCACGCGGC CCGGCCCGGC GCTTCCCCGG TGGCAGAAGG CCTCGGCCTC 
CCTCCGAAAC CCACCGTCCC GGACTTGACG GCGCCCGTCC AGGCAATCGC TTTGCGACCC 
CAAGCTCCGG CCGCTCAGCT CGCCCAGGCG TGCGGGGCGC GGGAGAGCAT CAAGCGGGAG 
GCTTCCCTGG CGCCCCTGGC CACCTCCCCG CCGCCGGGCA AGTGGGGAAC GCCCAAGAAG 
GGCAAGGAGC AGCCGGACCG CGCGCACAGT CAATTCCTGG GGATAGTGGG CGGCTCCTCG 
GGCGGCGGCG GAGGCCTGCC ATTCTACCCA GGCGTGCGCT CGGCTTTCAA GCCGGCCGGC 
CTGGCCCGGG CGGCTGCGCA GAGCGACCCC TACCGGGAAG AGGGCGGCGG CAAGGGACCG 
GGGCTGGCGC TAGGGCGGCT GCTGGGCGGG GGCCGGGCCG GCGGGCGTCC GGGCAGTGGG 
GAGAGCCCGG CGGGCCACCA CCACCATCAC CACCACGCTC ACCACCACCA CCACCACCAT 
CCCAAGTGCC TGCTGGCCGG GGAGCCGCCG CCGGCGGGCC TGCCGTGTCC CGGGGCCCTG 
AGAGCCTTCC CCCTGCTCGC CGGCCACCCG GAAGAGGCGT CGGCCTTCAA GCACGTGGAG 
CGGGCTCCGC CCGCCGCCGC CACCACCTCC CTGCCCTCCG CGCGCTACGC CGCTCTCCCG 
GCGCCCGGGC TCCCTGTGGA GCGCTGCGCG CTGCAGCCCC TCGACGGCGG CAGCCTCAAG 
GCTTACCCCG GCGGCGGTGG CGGCGGCGAG TGCAGCCCCC TGCCCGCCGT CATGCCGGCC 
TTCACGGTGT ACAGCGGAGA CCTGCTCTAC GGCCCTCCGG CCGCCTATTA CCCTCTCAAG 
CTGCACTTGG GCGGGCTGCT CAAATACCCC GAGTCCATCT CCTATCTGAG TGGCCCCGCG 
GCGGCAGCGG CGGCGGCGGC AGCGGCAGCG GCGGCGGCGG CGGCGATCGG GCCGGCCGAA 
CTGGGCTCCC TGGCCAGCAT CGACCGCGAG ATCGCCATGC ACACACAGCA GCTGTCCGAG 
ATGGCAGCCG GGAAGAGTCG CGCCCGCCTG GACTCGGGGA CGCTGCCTCC AGCCGTAGTG 
GCGGCGACGG GCCCCGGGGG TGGCGGCGGT GGCGGGAGCG CGGCAGGCAA GCCCAAGACA 
GGCCACTTGT GCCTCTACTG TGGCAAGCTG TACTCGCGCA AGTACGGGCT CAAGATCCAC 
ATGCGGACGC ACACGGGCTA CAAGCCGCTC AAGTGCAAAG TGTGCCTGCG GCCCTTCGGC 
GACCCCAGCA ATCTCAACAA GCACATCCGA CTCCACGCCG AGGGCAATAC GCCCTACCGC 
TGCGAGTTCT GCGGCAAGGT GCTGGTGCGC CGCCGAGACC TGGAGCGTCA CGTCAAGTCC 
CGCCACCCAG GCCAGAGCCT GATGGCCAAG GCGGGCGACG GCCCGGGCCC GGAGCCCAGC 
TATGCTCTGG AGCCTGGGGA TCCCAAGAGC GAGGACAGTG ATGTGGATGT CTGCTTCACT 
GACGATCAGA GCGACCCCGA GGCTGGGGGT CGTGGCGAGC ACGACTCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Prdm13 is required for Ebf3+ amacrine cell formation in the retina.
Developmental biology(2017 Dec)
Goodson NB,Nahreini J,Randazzo G,Uruena A,Johnson JE,Brzezinski JA 4th

Repression by PRDM13 is critical for generating precision in neuronal identity.
eLife6(2017 Aug)
Mona B,Uruena A,Kollipara RK,Ma Z,Borromeo MD,Chang JC,Johnson JE

Prdm13 regulates subtype specification of retinal amacrine interneurons and modulates visual sensitivity.
The Journal of neuroscience : the official journal of the Society for Neuroscience35(20)8004-20(2015 May)
Watanabe S,Sanuki R,Sugita Y,Imai W,Yamazaki R,Kozuka T,Ohsuga M,Furukawa T

Deficiency of Prdm13, a dorsomedial hypothalamus-enriched gene, mimics age-associated changes in sleep quality and adiposity.
Aging cell14(2)209-18(2015 Apr)
Satoh A,Brace CS,Rensing N,Imai S

The Prdm13 histone methyltransferase encoding gene is a Ptf1a-Rbpj downstream target that suppresses glutamatergic and promotes GABAergic neuronal fate in the dorsal neural tube.
Developmental biology386(2)340-57(2014 Feb)
Hanotel J,Bessodes N,Th?lie A,Hedderich M,Parain K,Van Driessche B,Brand?o Kde O,Kricha S,Jorgensen MC,Grapin-Botton A,Serup P,Van Lint C,Perron M,Pieler T,Henningfeld KA,Bellefroid EJ

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Prdm13 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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