Cenpe cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cenpe
Entrez Gene ID	229841
Full Name	centromere protein E
Synonyms	312kDa,AU019344,BC049989,C530022J18,CENP-E,Kif10
General protein information	Preferred Names centromere protein E Names centromere-associated protein E KIF10 N-7 kinesin cenpe centromere autoantigen E centromeric protein E kinesin 10 kinesin superfamily protein 10 motor domain of KIF10
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3 G3\|3 62.55 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_017319565.1	XP_017175054.1	centromere-associated protein E isoform X1
NM_173762.4	NP_776123.3	centromere-associated protein E

Pathways from BioSystems

Homologies

Bos taurus (cattle)	CENPE	XP_002688141.1
	CENPE	XP_001110512.1
Canis lupus familiaris (dog)	CENPE	XP_005639295.1
Homo sapiens (human)	CENPE	NP_001804.2
Pan troglodytes (chimpanzee)	CENPE	XP_001170275.1
Mus musculus (house mouse)	Cenpe	NP_776123.3
Rattus norvegicus (Norway rat)	Cenpe	XP_006233412.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

The Interleukin-2-mTORc1 Kinase Axis Defines the Signaling, Differentiation, and Metabolism of T Helper 1 and Follicular B Helper T Cells.
Ray JP, Staron MM, Shyer JA, Ho PC, Marshall HD, Gray SM, Laidlaw BJ, Araki K, Ahmed R, Kaech SM, Craft J
Immunity43(4)690-702(2015 Oct)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

Conserved molecular signatures of neurogenesis in the hippocampal subgranular zone of rodents and primates.
Miller JA, Nathanson J, Franjic D, Shim S, Dalley RA, Shapouri S, Smith KA, Sunkin SM, Bernard A, Bennett JL, Lee CK, Hawrylycz MJ, Jones AR, Amaral DG, ?estan N, Gage FH, Lein ES
Development (Cambridge, England)140(22)4633-44(2013 Nov)

Chromosome missegregation rate predicts whether aneuploidy will promote or suppress tumors.
Silk AD, Zasadil LM, Holland AJ, Vitre B, Cleveland DW, Weaver BA
Proceedings of the National Academy of Sciences of the United States of America110(44)E4134-41(2013 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Exacerbating chromosome missegregation in CENP-E+/- mice by reducing levels of another mitotic checkpoint component, Mad2, results in elevated cell death and decreased tumor formation compared with reduction of either protein alone.
Title: Chromosome missegregation rate predicts whether aneuploidy will promote or suppress tumors.

CENP-E stabilises BubR1, thereby impacting meiosis I progression, and mediates bi-orientation by promoting kinetochore reorientation and preventing chromosomal drift towards the poles.
Title: Spindle assembly checkpoint signalling is uncoupled from chromosomal position in mouse oocytes.

Shugoshin 2 is necessary for the loading of MCAK at the inner centromere, but is dispensable for the loading of the outer kinetochore proteins BubR1 and CENP-E.
Title: Sequential assembly of centromeric proteins in male mouse meiosis.

Deletion of the mouse gene CENP-E causes unstable kinetochore-microtubule capture and chromosomal instability. (CENP-E)
Title: Unstable kinetochore-microtubule capture and chromosomal instability following deletion of CENP-E.

results suggest that CENP-E is implicated in the spindle checkpoint, and in chromosome alignment, during the two meiotic divisions in vertebrate males
Title: Expression and behaviour of CENP-E at kinetochores during mouse spermatogenesis.

The following Cenpe gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cenpe cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu80610	XM_017319565.1 Latest version!	Mus musculus centromere protein E (Cenpe), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu21751	NM_173762.4 Latest version!	Mus musculus centromere protein E (Cenpe), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1819.30 $2599.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cenpe ( XM_017319565.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175054.1 Mus musculus centromere protein E (Cenpe), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu80610
Clone ID Related Accession (Same CDS sequence)	XM_017319565.1
Accession Version	XM_017319565.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7413bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	centromere-associated protein E isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG AGGCTTCAGT GGCTGTCTGT GTTCGTGTGC GACCCCTCAA CAGCAGAGAA 
GAAGAACTTG GAGAAGCCAC CCACATTTAC TGGAAAACAG ATAAAAACGC TATTTATCAG 
AGCGATGGGG GTAAATCTTT CCAATTTGAT CGTGTCTTTG ACAGTAATGA AACCACGAAG 
AATGTGTATG AAGAGATTGC GGTGCCCATC ATCAGCTCCG CCATACAAGG CTACAATGGT 
ACTATATTTG CCTATGGACA AACTGCATCA GGAAAGACAC ACACGATGAT GGGTTCTGAA 
GATTGTTTGG GAGTTATACC CAGGGCAATT CATGACATTT TCCAAAGGAT AAAGAAGTTT 
CCTGAGAGAG AATTTCTCCT ACGAGTTTCT TACATGGAGA TCTACAATGA AACCATTACA 
GATTTACTCT GTAATGCTCA AAAAATGAAA CCTTTGATAA TTCGGGAAGA CACCAATAGG 
ACTGTATATG TTTCTGATCT CACAGAAGAA GTGGTTTATA CAGCAGAAAT GGCTTTGAAA 
TGGCTCGCAA CAGGAGAAAA AAACAGACAT TATGGAATAA CCAAAATGAA TCAAAGAAGC 
AGTCGTTCTC ATACGATTTT TAGGATGATT CTGGAAAGTA GAGAAAAGGC TGAGCCTTCT 
AACTGCGATG GCTCCGTCAA AGTGTCTCAT CTGAATTTGG TCGATCTTGC AGGCAGTGAA 
AGAGCTGCTC AAACAGGAGC TGAAGGTGTG CGATTGAAGG AGGGCTGTTT TATAAATCGG 
AACTTGTTTA TTTTGGGACA AGTGATTAAG AAACTCAGTG ATGGACAGGT TGGGGGTTTC 
ATAAATTACC GAGATAGCAA ATTGACACGA ATTCTTCAGA ATTCCTTGGG AGGAAATGCC 
AAGACGCGCA TCATCTGCAC GATCACCCCA GCGTCCCTGG ACGAAACCCT GACGACGCTG 
CAGTTTGCCA GCACTGCTAA GTATATGAAG AATACTCCTT ACGTTAATGA AGTGTCGAAT 
GATGAAGCTC TCTTGAAAAG ATACAGAAGA GAAATAGCGG ATCTTAGAAA ACAATTAGAG 
GAGGTAAATA CAAAGACTCG GGCACAGGAA ATGGAAAAGG ATCAACTGGC CCAGCTTCTG 
GATGAAAAAG ACTTGCTTCA GAAAGTTCAG GATGAGAAAA TTAATAACCT GAAGCGGATG 
CTGGTGACGT CATCTTCTAT TGCATTGCAG CAGGAGCTAG AGATTAAAAG GAAACGAAGA 
GTTACTTGGT GCTATGGCAA AATGAAGGAT TCAAACTATG AAAAGGAATT TAAAGTCCCG 
ACAAGCATAA CAACAAGAAA ACGTAAAACT TCTGTGACTT CTTTGAGAGA AAATTCTCTC 
ATGAAGTTCG GAGAGAGTGC CTCTTCCGAG TTTGAAATGT TGAATAACAC TCTTGAGTCA 
TTGGCTGAGG TAGAGTGGAG CTCGGCAACA ACGCTCCTAA GCGAGGAAAA TGTAGAAAGT 
GAGTTGAACT CACTTAATGC TCAATATAAT GATCTGGTAT TAGACTATGA ACAGCTCAGA 
AGAGAAAATG AAGACTTGAA ATTGAAATTA AAAGAAAAAA ATGAGTTAGA GGAATTTGAG 
CTTCTAGAAC AAAAAGAAGA AAGAGATCAA GAGATGCAAC TAATGCATGA AGTTTCCAAC 
TTAAAGAATT TAATTAAACA CGCAGAAGAA TATAATCAAG ACCTTGAGAA TGACCTAAGT 
TCAAAAGTGA AGCTTCTTAA AGAAAAAGAG GAGCAGATTA AGAACCTGCA GGAATACATA 
GATGCCCAGA AGTCAGAAAA GATGAAGATA GACCTGTCAT ACACATCGGA CGCCACTGAA 
GACCTGAAAC AGGCGATGCG CACCCTGTCT GATTTGGACA CAGTGGCCCT TGACGCTAAG 
AAAGAGTCAG CCTTCCTTAG AAGTGAAAAT CTGGAGCTGA AGGAGAAAAT TAATGAACTT 
TCGGATTCCC GTAAGCAAAT GGAAAGTGAC ATTCAGATGT ATCAACGCCA ACTGGAAGCA 
AAAAAGAAAA TGCAAACTGA TCTGGATAAG GAATTACAGT TGGCTTTTCA AGAAATATCA 
AAACTCAGCG CCCTGGTAGA TGGCAAAGGT TTACTGTCTA ATTTGGAATT GGAGAAAAGG 
ATTACTGATC TCCAAAAAGA ACTAAATAAA GAAGCTGAAG AAAAACAAAC TTTGCAAGAA 
GAAGTTAACT TGCTGTCGGA GTTGAAATCT TTACCGTCTG AGGTGGAAAC GCTGAGGAGA 
GAGCTGTATG AGAAATCTGA AGAGCTCCAT ATAATAACCA CAGAAAGAGA GAAACTGTTT 
TCTGAAATGG CTCATAAAGA CAGTAGGATT CAAGGTTTAC TTGAGGAAAT TGGAAACACT 
AGAGATGACC TTGCCACTTC CCAGCTGAGT CGGAGGGGCA GTGATGGAGA ATGGCAAGCC 
CTGGAAAGCC TGCACGCAGA GCTGGAGCAC AGACACGCGG GGGTCCTGGA GGAGAGAGAG 
AGGCTGAAGC AGGAGATTGG GGCTCTCTCC AAAGAAGCAG AAAGCCTGGC TTTCAGTTTG 
GATTCCGTGA AGGCCGAGCT TTCTCACAAG ACTCAAGAGC TTGAGCAGAA AACAGTTGAG 
GGTCAAGAAA GGCTAAATAA AATGGAGGCG CTGAGAGAAG AGTTAGAAAG CAGAGACTCC 
AGCCTGCAGT CTGTAGAGAA GGAGAAAGTC CTGCTCACTG AGAAGCTGCA GCAAGCCTTG 
AAGGAAGTAA AAGCCTTAAC CCAAGAAAAG AAAAACCTGA AGCAGCTCCA GGAGAGCCTG 
CAAACTGAGA GAGACCAACT CAGAAGTGAC ATTCAGGACA CTGTGAACAT GAACATAGAT 
ACTCAAGAGC AGTTACTAAA TGCTCTGGAG TCTCTGAAAC AACACCAAGA AACAATTAAC 
ATGCTGAAAA TGAAAGCTGC TGAGGAACTA TCCGATAATT TGCATGTAAA GGACAGGGGA 
GGAGCTAGAG ATGAAGCTCA GCAAAAGATG GATGGCATAG ATGAACAGAA TGAGAGTGCC 
CACACATTGC TTGGTGGTGG TAAGGACAAT GAGGTAACTG AGGAACAGAG GAAAATCGAC 
TCTCTAATGC AGGAGAATAG CGGACTCCAA CAGACACTGG AAAGTGTTAG AGCAGAAAAG 
GAACAACTGA AGATGGACCT AAAGGAAAAT ATCGAAATGA GTATTGAAAA CCAGGAAGAG 
TTAAGAATTC TTAGAGATGA ACTGAAAAGA CAACAAGAGG TAGCTGCACA GGAAAAGGAT 
CATGCCACCG AGAAGACCCA GGAGCTCTCC AGGACGCAGG AAAGATTGGC AAAAACAGAA 
GAGAAGTTAG AGGAAAAGAA CCAGAAACTC CAAGAGACAC AGCAGCAACT TCTTAGTACA 
CAAGAAGCTA TGAGTAAGCT GCAGGCAAAG GTGATTGACA TGGAAAGCTT ACAGAATGAA 
TTCAGGAACC AAGGATTGGC ATTGGAGCGT GTGGAGACAG AGAAACTGGA GTTAGCTCAG 
AGACTTCATG AAAGTTATGA GGAGGTGAAA TCTATAACCA AAGAAAGAAA TGATCTAAAG 
GAATTGCAGG AGTCATTTGA AATAGAGAAG AAGCAACTTA AAGAATATGC AAGGGAAATA 
GAAGCCGCGG GCCTACAAAC AAAAGAAGAA CTAAACATTG CCCACGCGAA TCTAAAAGAG 
TACCAGGAAA TCATCACAGA GCTAAGAGGA AGCATTTCTG AAAACGAAGC ACAAGGAGCA 
AGCACCCAGG ACACGGCAAA AAGCGCCCCT GAGCTACAAG GAGAGGTTCC AGAGCAAGAG 
CTGCTTCCTG TTGTCAAAGA AGCCAGGCAC AGTGCAGAAA AAGTGAACGG ACTGGAGCCA 
GTGGGAGCAC ATTCCAGAAC AGTGCACTCC ATGACAATGG AGGGCATAGA AATAGAAAAC 
CTCAGGCTGA CCAAGAAACT TGAAGAAAGT CAGATGGAGA TCAGCTGTCT CACTAGAGAG 
AGAGAAGACC TGAGAAGGAC ACAGGAAACA CTTCAGGTCG AATGTACCCA GCTGAAAGAA 
GATGCCAGAA GAACTTTGGC TAATCATCTA GAAACTGAAG AGGAACTTAA CCTTGCTCGT 
TGTTGCCTGA AAGAGCAGGA GAACAAGATT GACACCCTAA TAACGAGCCT CTCGCAGAGG 
GAAACTGAGC TGTCCAGTGT GCGAGGACAA CTAGCGCTGA CTACTGCTGA ACTGGAGAGA 
AAGGTTCAAG AACTCTGTGA GAAACAGGAA GAACTTACTA GGAAAGAAAC TAGCGAGGCT 
CAAGGGAAGA TGAGTGAACT AGAGCAACTC AGGGAGCTGC TGCTTGCCCA GGCCTCGGCA 
CTGCAGAACG CAGAGAGTGA CAGGCTGCGG CTGAATACAC AACTGGAAGA AAGTCAAGAG 
GAAATGAAAA CTCTAAGGGA GGAAAGAGAA GAGCTGAGAA GAATGCAAGA GGCTCTTCAC 
GTGGAAAGTG AGCAGCAGAA AGAAAGCATG AAGGAAATCA GCTCAAAACT CCAAGAACTA 
CAGAACAAAG AGTATGAATG TCTTGCAATG AAGACTATTA ATGAGACTCA AGGAAGCAGA 
TGTGAAATGG ACCACTTGAA CCAGCAGCTG GAGGCCCAGA AGTCAACTCT GGAAAAGGTG 
GAGATGGAGA ATGTAAACTT GACTCAAAGA CTTCATGAGA CCCTTGAGGA AATGAGATCT 
GTAGCAAAAG AAAGAGATGA GCTTTGGAGC ATGGAGGAGC GCCTCACAGT AGAGAGAGAC 
CAACTGAAGA AGAGCCTTGA AGAAACAGTA ACTAAAGGTA TGGAAAAAGA GGAAGAGCTA 
AGAGTTGCGC ATGTGCATCT GGAAGAGCAC CAGGAAACTA TCAACAAGCT CAGAAAGATG 
GTGTCTGACT ACACAGATGA AATATCACAT ACTCAAGGGG ACCTAAAGCA CACAAATGCC 
GTTGTAGAAG CTCAGAACCA AGATCTCCGG GAAAAAGAAC ATCAACTTTC TCAAGTAAAA 
GCTGATCTCA GAGAAACCGT GGATCAAATG GAGCAACTGA AGAAGAAGCT CGAAGCCCAG 
AGTTCGACTC TAGAAAGCAG AGAAATAGAG AAGTTAGAAT TGACTCAGCA ACTTAACGAA 
AACCTTAAAA AAATAACACT TGTCACTAAG GAAAATGATA GCCTAAAAAT TATGGATGAA 
GCCCTCAGGG AAGAACGAGA CCAGCTACGG AAAAGTCTTC AGCAAACAGA GGCAAGGGAT 
CTGGAAAATC AAGAGAAACT AAGAATTGCT CACATGAATC TAAAAGAGCA CCAAGAAACT 
ATTGACCGAC TCATGGAGAC TATGTCTGAG AAGACAGAGG AAATATCAAA TATGAAAATG 
GAGTTAGAAA ATGTAAACAT GAAATTACAA GAAAAGGTTC AAGAACTTAA GACAAGTGAG 
CGTCAACGTG TTAAGTTAAA AGCAGATGCC AGTGAAGCCA AGAAGGAGCT CAAGGAGCAG 
GGCTTAACTC TGAGTAAGAT AGAGATGGAG AACTTAAATC TCGCTCAGAA GATTCATGAG 
AACCTTGAAG AAATGAAATC TGTAAGAAAA GAAAGAGATG ATCTAAAGAA ACTTGAGGAG 
ATACTCAGAA TGGAGAGAGA CCAGCTCAAG GACAACCTGA GAGAAGCCAT GCTTAAGGCC 
CACCAGAACC ATGAAGAGAC AATGAAATGT GGAAAGGGGT TACTGTGTGC TGGAGAATAC 
TGTACAGGAA GACTTCGAGA AAAGTGCTTC AGGATAGAAA AACTCCTGAA AAGATACTCA 
GAAATGGCTA ATGATTATGA GTGCTTGAAT AAAGTCTCTC TTGACTTGGA GAGAGAAACT 
AAAACCCAAA AAGAGCTGTC AGTTACTGTA AGAACAAAGC TCTCACTTCC GCACACACAG 
ACCAAAGAGA TGGAAAAGCT TCTGACTGCA AACCAGAGAT GTTCCCTGGA GTTCCACAGA 
GCCCTCAAGA GACTTAAGTA CGTGTTAAGC TCCATTGCGA GGATAAAGGA AGAGCAGCAT 
GAGTCCATCA ATAAACGTGA AATGGCTTTT ATTCAGGAAG TGGAAAAGCA GAATGAACTG 
CAGATTCAAA TCCAGAGCCT TTCACAGACT TACCGTATTC CAGCCAGAGA CTTACAGATC 
AAACTGAGCC AGGAGATGGA CCTCCATATT GAGGAAATGC TCAAAGATTT TTCAGAAAAT 
GATTTCCTCA CCATAAAAAC TGAAGTCCAA CAAGTATTAA ATAATAGGAA AGAAATAACT 
GAGTTTTTGG GAAAATGGCT AAATACTCTT TTTGATACAG AAAATCTTAA GAGCACCATC 
CAGAAAGAAA ACAAAAGCAT TGGGCTCGTG AATAACTTCT ATCACAGCAG AATCACTGCC 
ATGATAAATG AATCAACGGA GTTTGAGGAA AGAAGTGCTA CCAGATCCAA AGACTTGGAT 
CAGTACCTGA AGTCACTGAA AGAAACAACT GAGCAACTGT CTGAAGTCTA CCAGACACTG 
ACCGCCTCCC AGTCTGTTGT GCATCTCCAT CCTACCGTGC AGCCTTCCAC ACGGGACAGT 
GAGCGTCCTC AGGCTGCATC AGGAGCTGAA CAACTGACCT CAAAGAACAA AATTGCTTTA 
GGAGCTGTGC CCTATAAAGA AGAAATTGAG GATCTCAAAA TGCAGCTGGT GAAGAGTGAC 
CTAGAGAAGA AGGCGACCGC CAAGGAATTC GATAAGAAAA TTCTCTCTCT AAAAGCCACT 
GTTGAACATC AAGAAGAAAT GATAAGGCTA CTGAGAGAAA ATCTTAGAGG ACATCAACAG 
GCCCAGGATA CCTCAATGAT CTCAGAACAA GACTCTCAGC TTCTGAGTAA ACCTCTAACC 
TGTGGAGGTG GCAGTGGAAT TGTACAAAGC ACAAAAGCTC TTATTTTGAA AAGTGAATAT 
AAAAGGATGG GAAGTGAGAT TTCTAAGTTA AAGCAGCAAA ATGAGCAGCT AAGAAAGCAA 
AATAATCAAC TATTAAGTGA TAACTCTCAA CTCTCCAATG AGGTTAAAAC CTGGGAGGAA 
AGAACTCTTA AAAGAGACAG TTACAGAGAA ACAACTTGTG AGAATTCTCC AAAGTCTCCT 
AAAGTGACTC GGACAGATTC TAAAAGGAGA CAGAACACAA CTTCTCAGTG CAGGGCACAA 
AATCTCCAGG ATCCTGTGCC AAAAGATTCC CCAAAATCAT GGTTTTTTGA TAACCGGTCA 
AAGTCTCTTC CAGCACCTCA CCCGATCCGC TATTTTGACA ACTCCAGTCT AGGGCTGTGC 
CCAGAACCAG ATGACGTAGA GAATGTGGAG CCTAAGACAG ACCTCTGCCA AGCCTCCTTG 
GAAAAAGATG TGTCCCAGTG CAAGACGCAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175054.1
CDS	123..7535
Translation	MAEEASVAVCVRVRPLNSREEELGEATHIYWKTDKNAIYQSDGGKSFQFDRVFDSNETTKNVYEEIAVPIISSAIQGYNGTIFAYGQTASGKTHTMMGSEDCLGVIPRAIHDIFQRIKKFPEREFLLRVSYMEIYNETITDLLCNAQKMKPLIIREDTNRTVYVSDLTEEVVYTAEMALKWLATGEKNRHYGITKMNQRSSRSHTIFRMILESREKAEPSNCDGSVKVSHLNLVDLAGSERAAQTGAEGVRLKEGCFINRNLFILGQVIKKLSDGQVGGFINYRDSKLTRILQNSLGGNAKTRIICTITPASLDETLTTLQFASTAKYMKNTPYVNEVSNDEALLKRYRREIADLRKQLEEVNTKTRAQEMEKDQLAQLLDEKDLLQKVQDEKINNLKRMLVTSSSIALQQELEIKRKRRVTWCYGKMKDSNYEKEFKVPTSITTRKRKTSVTSLRENSLMKFGESASSEFEMLNNTLESLAEVEWSSATTLLSEENVESELNSLNAQYNDLVLDYEQLRRENEDLKLKLKEKNELEEFELLEQKEERDQEMQLMHEVSNLKNLIKHAEEYNQDLENDLSSKVKLLKEKEEQIKNLQEYIDAQKSEKMKIDLSYTSDATEDLKQAMRTLSDLDTVALDAKKESAFLRSENLELKEKINELSDSRKQMESDIQMYQRQLEAKKKMQTDLDKELQLAFQEISKLSALVDGKGLLSNLELEKRITDLQKELNKEAEEKQTLQEEVNLLSELKSLPSEVETLRRELYEKSEELHIITTEREKLFSEMAHKDSRIQGLLEEIGNTRDDLATSQLSRRGSDGEWQALESLHAELEHRHAGVLEERERLKQEIGALSKEAESLAFSLDSVKAELSHKTQELEQKTVEGQERLNKMEALREELESRDSSLQSVEKEKVLLTEKLQQALKEVKALTQEKKNLKQLQESLQTERDQLRSDIQDTVNMNIDTQEQLLNALESLKQHQETINMLKMKAAEELSDNLHVKDRGGARDEAQQKMDGIDEQNESAHTLLGGGKDNEVTEEQRKIDSLMQENSGLQQTLESVRAEKEQLKMDLKENIEMSIENQEELRILRDELKRQQEVAAQEKDHATEKTQELSRTQERLAKTEEKLEEKNQKLQETQQQLLSTQEAMSKLQAKVIDMESLQNEFRNQGLALERVETEKLELAQRLHESYEEVKSITKERNDLKELQESFEIEKKQLKEYAREIEAAGLQTKEELNIAHANLKEYQEIITELRGSISENEAQGASTQDTAKSAPELQGEVPEQELLPVVKEARHSAEKVNGLEPVGAHSRTVHSMTMEGIEIENLRLTKKLEESQMEISCLTREREDLRRTQETLQVECTQLKEDARRTLANHLETEEELNLARCCLKEQENKIDTLITSLSQRETELSSVRGQLALTTAELERKVQELCEKQEELTRKETSEAQGKMSELEQLRELLLAQASALQNAESDRLRLNTQLEESQEEMKTLREEREELRRMQEALHVESEQQKESMKEISSKLQELQNKEYECLAMKTINETQGSRCEMDHLNQQLEAQKSTLEKVEMENVNLTQRLHETLEEMRSVAKERDELWSMEERLTVERDQLKKSLEETVTKGMEKEEELRVAHVHLEEHQETINKLRKMVSDYTDEISHTQGDLKHTNAVVEAQNQDLREKEHQLSQVKADLRETVDQMEQLKKKLEAQSSTLESREIEKLELTQQLNENLKKITLVTKENDSLKIMDEALREERDQLRKSLQQTEARDLENQEKLRIAHMNLKEHQETIDRLMETMSEKTEEISNMKMELENVNMKLQEKVQELKTSERQRVKLKADASEAKKELKEQGLTLSKIEMENLNLAQKIHENLEEMKSVRKERDDLKKLEEILRMERDQLKDNLREAMLKAHQNHEETMKCGKGLLCAGEYCTGRLREKCFRIEKLLKRYSEMANDYECLNKVSLDLERETKTQKELSVTVRTKLSLPHTQTKEMEKLLTANQRCSLEFHRALKRLKYVLSSIARIKEEQHESINKREMAFIQEVEKQNELQIQIQSLSQTYRIPARDLQIKLSQEMDLHIEEMLKDFSENDFLTIKTEVQQVLNNRKEITEFLGKWLNTLFDTENLKSTIQKENKSIGLVNNFYHSRITAMINESTEFEERSATRSKDLDQYLKSLKETTEQLSEVYQTLTASQSVVHLHPTVQPSTRDSERPQAASGAEQLTSKNKIALGAVPYKEEIEDLKMQLVKSDLEKKATAKEFDKKILSLKATVEHQEEMIRLLRENLRGHQQAQDTSMISEQDSQLLSKPLTCGGGSGIVQSTKALILKSEYKRMGSEISKLKQQNEQLRKQNNQLLSDNSQLSNEVKTWEERTLKRDSYRETTCENSPKSPKVTRTDSKRRQNTTSQCRAQNLQDPVPKDSPKSWFFDNRSKSLPAPHPIRYFDNSSLGLCPEPDDVENVEPKTDLCQASLEKDVSQCKTQ

Target ORF information:

RefSeq Version	XM_017319565.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus centromere protein E (Cenpe), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319565.1

ORF Insert Sequence:

ATGGCCGAGG AGGCTTCAGT GGCTGTCTGT GTTCGTGTGC GACCCCTCAA CAGCAGAGAA 
GAAGAACTTG GAGAAGCCAC CCACATTTAC TGGAAAACAG ATAAAAACGC TATTTATCAG 
AGCGATGGGG GTAAATCTTT CCAATTTGAT CGTGTCTTTG ACAGTAATGA AACCACGAAG 
AATGTGTATG AAGAGATTGC GGTGCCCATC ATCAGCTCCG CCATACAAGG CTACAATGGT 
ACTATATTTG CCTATGGACA AACTGCATCA GGAAAGACAC ACACGATGAT GGGTTCTGAA 
GATTGTTTGG GAGTTATACC CAGGGCAATT CATGACATTT TCCAAAGGAT AAAGAAGTTT 
CCTGAGAGAG AATTTCTCCT ACGAGTTTCT TACATGGAGA TCTACAATGA AACCATTACA 
GATTTACTCT GTAATGCTCA AAAAATGAAA CCTTTGATAA TTCGGGAAGA CACCAATAGG 
ACTGTATATG TTTCTGATCT CACAGAAGAA GTGGTTTATA CAGCAGAAAT GGCTTTGAAA 
TGGCTCGCAA CAGGAGAAAA AAACAGACAT TATGGAATAA CCAAAATGAA TCAAAGAAGC 
AGTCGTTCTC ATACGATTTT TAGGATGATT CTGGAAAGTA GAGAAAAGGC TGAGCCTTCT 
AACTGCGATG GCTCCGTCAA AGTGTCTCAT CTGAATTTGG TCGATCTTGC AGGCAGTGAA 
AGAGCTGCTC AAACAGGAGC TGAAGGTGTG CGATTGAAGG AGGGCTGTTT TATAAATCGG 
AACTTGTTTA TTTTGGGACA AGTGATTAAG AAACTCAGTG ATGGACAGGT TGGGGGTTTC 
ATAAATTACC GAGATAGCAA ATTGACACGA ATTCTTCAGA ATTCCTTGGG AGGAAATGCC 
AAGACGCGCA TCATCTGCAC GATCACCCCA GCGTCCCTGG ACGAAACCCT GACGACGCTG 
CAGTTTGCCA GCACTGCTAA GTATATGAAG AATACTCCTT ACGTTAATGA AGTGTCGAAT 
GATGAAGCTC TCTTGAAAAG ATACAGAAGA GAAATAGCGG ATCTTAGAAA ACAATTAGAG 
GAGGTAAATA CAAAGACTCG GGCACAGGAA ATGGAAAAGG ATCAACTGGC CCAGCTTCTG 
GATGAAAAAG ACTTGCTTCA GAAAGTTCAG GATGAGAAAA TTAATAACCT GAAGCGGATG 
CTGGTGACGT CATCTTCTAT TGCATTGCAG CAGGAGCTAG AGATTAAAAG GAAACGAAGA 
GTTACTTGGT GCTATGGCAA AATGAAGGAT TCAAACTATG AAAAGGAATT TAAAGTCCCG 
ACAAGCATAA CAACAAGAAA ACGTAAAACT TCTGTGACTT CTTTGAGAGA AAATTCTCTC 
ATGAAGTTCG GAGAGAGTGC CTCTTCCGAG TTTGAAATGT TGAATAACAC TCTTGAGTCA 
TTGGCTGAGG TAGAGTGGAG CTCGGCAACA ACGCTCCTAA GCGAGGAAAA TGTAGAAAGT 
GAGTTGAACT CACTTAATGC TCAATATAAT GATCTGGTAT TAGACTATGA ACAGCTCAGA 
AGAGAAAATG AAGACTTGAA ATTGAAATTA AAAGAAAAAA ATGAGTTAGA GGAATTTGAG 
CTTCTAGAAC AAAAAGAAGA AAGAGATCAA GAGATGCAAC TAATGCATGA AGTTTCCAAC 
TTAAAGAATT TAATTAAACA CGCAGAAGAA TATAATCAAG ACCTTGAGAA TGACCTAAGT 
TCAAAAGTGA AGCTTCTTAA AGAAAAAGAG GAGCAGATTA AGAACCTGCA GGAATACATA 
GATGCCCAGA AGTCAGAAAA GATGAAGATA GACCTGTCAT ACACATCGGA CGCCACTGAA 
GACCTGAAAC AGGCGATGCG CACCCTGTCT GATTTGGACA CAGTGGCCCT TGACGCTAAG 
AAAGAGTCAG CCTTCCTTAG AAGTGAAAAT CTGGAGCTGA AGGAGAAAAT TAATGAACTT 
TCGGATTCCC GTAAGCAAAT GGAAAGTGAC ATTCAGATGT ATCAACGCCA ACTGGAAGCA 
AAAAAGAAAA TGCAAACTGA TCTGGATAAG GAATTACAGT TGGCTTTTCA AGAAATATCA 
AAACTCAGCG CCCTGGTAGA TGGCAAAGGT TTACTGTCTA ATTTGGAATT GGAGAAAAGG 
ATTACTGATC TCCAAAAAGA ACTAAATAAA GAAGCTGAAG AAAAACAAAC TTTGCAAGAA 
GAAGTTAACT TGCTGTCGGA GTTGAAATCT TTACCGTCTG AGGTGGAAAC GCTGAGGAGA 
GAGCTGTATG AGAAATCTGA AGAGCTCCAT ATAATAACCA CAGAAAGAGA GAAACTGTTT 
TCTGAAATGG CTCATAAAGA CAGTAGGATT CAAGGTTTAC TTGAGGAAAT TGGAAACACT 
AGAGATGACC TTGCCACTTC CCAGCTGAGT CGGAGGGGCA GTGATGGAGA ATGGCAAGCC 
CTGGAAAGCC TGCACGCAGA GCTGGAGCAC AGACACGCGG GGGTCCTGGA GGAGAGAGAG 
AGGCTGAAGC AGGAGATTGG GGCTCTCTCC AAAGAAGCAG AAAGCCTGGC TTTCAGTTTG 
GATTCCGTGA AGGCCGAGCT TTCTCACAAG ACTCAAGAGC TTGAGCAGAA AACAGTTGAG 
GGTCAAGAAA GGCTAAATAA AATGGAGGCG CTGAGAGAAG AGTTAGAAAG CAGAGACTCC 
AGCCTGCAGT CTGTAGAGAA GGAGAAAGTC CTGCTCACTG AGAAGCTGCA GCAAGCCTTG 
AAGGAAGTAA AAGCCTTAAC CCAAGAAAAG AAAAACCTGA AGCAGCTCCA GGAGAGCCTG 
CAAACTGAGA GAGACCAACT CAGAAGTGAC ATTCAGGACA CTGTGAACAT GAACATAGAT 
ACTCAAGAGC AGTTACTAAA TGCTCTGGAG TCTCTGAAAC AACACCAAGA AACAATTAAC 
ATGCTGAAAA TGAAAGCTGC TGAGGAACTA TCCGATAATT TGCATGTAAA GGACAGGGGA 
GGAGCTAGAG ATGAAGCTCA GCAAAAGATG GATGGCATAG ATGAACAGAA TGAGAGTGCC 
CACACATTGC TTGGTGGTGG TAAGGACAAT GAGGTAACTG AGGAACAGAG GAAAATCGAC 
TCTCTAATGC AGGAGAATAG CGGACTCCAA CAGACACTGG AAAGTGTTAG AGCAGAAAAG 
GAACAACTGA AGATGGACCT AAAGGAAAAT ATCGAAATGA GTATTGAAAA CCAGGAAGAG 
TTAAGAATTC TTAGAGATGA ACTGAAAAGA CAACAAGAGG TAGCTGCACA GGAAAAGGAT 
CATGCCACCG AGAAGACCCA GGAGCTCTCC AGGACGCAGG AAAGATTGGC AAAAACAGAA 
GAGAAGTTAG AGGAAAAGAA CCAGAAACTC CAAGAGACAC AGCAGCAACT TCTTAGTACA 
CAAGAAGCTA TGAGTAAGCT GCAGGCAAAG GTGATTGACA TGGAAAGCTT ACAGAATGAA 
TTCAGGAACC AAGGATTGGC ATTGGAGCGT GTGGAGACAG AGAAACTGGA GTTAGCTCAG 
AGACTTCATG AAAGTTATGA GGAGGTGAAA TCTATAACCA AAGAAAGAAA TGATCTAAAG 
GAATTGCAGG AGTCATTTGA AATAGAGAAG AAGCAACTTA AAGAATATGC AAGGGAAATA 
GAAGCCGCGG GCCTACAAAC AAAAGAAGAA CTAAACATTG CCCACGCGAA TCTAAAAGAG 
TACCAGGAAA TCATCACAGA GCTAAGAGGA AGCATTTCTG AAAACGAAGC ACAAGGAGCA 
AGCACCCAGG ACACGGCAAA AAGCGCCCCT GAGCTACAAG GAGAGGTTCC AGAGCAAGAG 
CTGCTTCCTG TTGTCAAAGA AGCCAGGCAC AGTGCAGAAA AAGTGAACGG ACTGGAGCCA 
GTGGGAGCAC ATTCCAGAAC AGTGCACTCC ATGACAATGG AGGGCATAGA AATAGAAAAC 
CTCAGGCTGA CCAAGAAACT TGAAGAAAGT CAGATGGAGA TCAGCTGTCT CACTAGAGAG 
AGAGAAGACC TGAGAAGGAC ACAGGAAACA CTTCAGGTCG AATGTACCCA GCTGAAAGAA 
GATGCCAGAA GAACTTTGGC TAATCATCTA GAAACTGAAG AGGAACTTAA CCTTGCTCGT 
TGTTGCCTGA AAGAGCAGGA GAACAAGATT GACACCCTAA TAACGAGCCT CTCGCAGAGG 
GAAACTGAGC TGTCCAGTGT GCGAGGACAA CTAGCGCTGA CTACTGCTGA ACTGGAGAGA 
AAGGTTCAAG AACTCTGTGA GAAACAGGAA GAACTTACTA GGAAAGAAAC TAGCGAGGCT 
CAAGGGAAGA TGAGTGAACT AGAGCAACTC AGGGAGCTGC TGCTTGCCCA GGCCTCGGCA 
CTGCAGAACG CAGAGAGTGA CAGGCTGCGG CTGAATACAC AACTGGAAGA AAGTCAAGAG 
GAAATGAAAA CTCTAAGGGA GGAAAGAGAA GAGCTGAGAA GAATGCAAGA GGCTCTTCAC 
GTGGAAAGTG AGCAGCAGAA AGAAAGCATG AAGGAAATCA GCTCAAAACT CCAAGAACTA 
CAGAACAAAG AGTATGAATG TCTTGCAATG AAGACTATTA ATGAGACTCA AGGAAGCAGA 
TGTGAAATGG ACCACTTGAA CCAGCAGCTG GAGGCCCAGA AGTCAACTCT GGAAAAGGTG 
GAGATGGAGA ATGTAAACTT GACTCAAAGA CTTCATGAGA CCCTTGAGGA AATGAGATCT 
GTAGCAAAAG AAAGAGATGA GCTTTGGAGC ATGGAGGAGC GCCTCACAGT AGAGAGAGAC 
CAACTGAAGA AGAGCCTTGA AGAAACAGTA ACTAAAGGTA TGGAAAAAGA GGAAGAGCTA 
AGAGTTGCGC ATGTGCATCT GGAAGAGCAC CAGGAAACTA TCAACAAGCT CAGAAAGATG 
GTGTCTGACT ACACAGATGA AATATCACAT ACTCAAGGGG ACCTAAAGCA CACAAATGCC 
GTTGTAGAAG CTCAGAACCA AGATCTCCGG GAAAAAGAAC ATCAACTTTC TCAAGTAAAA 
GCTGATCTCA GAGAAACCGT GGATCAAATG GAGCAACTGA AGAAGAAGCT CGAAGCCCAG 
AGTTCGACTC TAGAAAGCAG AGAAATAGAG AAGTTAGAAT TGACTCAGCA ACTTAACGAA 
AACCTTAAAA AAATAACACT TGTCACTAAG GAAAATGATA GCCTAAAAAT TATGGATGAA 
GCCCTCAGGG AAGAACGAGA CCAGCTACGG AAAAGTCTTC AGCAAACAGA GGCAAGGGAT 
CTGGAAAATC AAGAGAAACT AAGAATTGCT CACATGAATC TAAAAGAGCA CCAAGAAACT 
ATTGACCGAC TCATGGAGAC TATGTCTGAG AAGACAGAGG AAATATCAAA TATGAAAATG 
GAGTTAGAAA ATGTAAACAT GAAATTACAA GAAAAGGTTC AAGAACTTAA GACAAGTGAG 
CGTCAACGTG TTAAGTTAAA AGCAGATGCC AGTGAAGCCA AGAAGGAGCT CAAGGAGCAG 
GGCTTAACTC TGAGTAAGAT AGAGATGGAG AACTTAAATC TCGCTCAGAA GATTCATGAG 
AACCTTGAAG AAATGAAATC TGTAAGAAAA GAAAGAGATG ATCTAAAGAA ACTTGAGGAG 
ATACTCAGAA TGGAGAGAGA CCAGCTCAAG GACAACCTGA GAGAAGCCAT GCTTAAGGCC 
CACCAGAACC ATGAAGAGAC AATGAAATGT GGAAAGGGGT TACTGTGTGC TGGAGAATAC 
TGTACAGGAA GACTTCGAGA AAAGTGCTTC AGGATAGAAA AACTCCTGAA AAGATACTCA 
GAAATGGCTA ATGATTATGA GTGCTTGAAT AAAGTCTCTC TTGACTTGGA GAGAGAAACT 
AAAACCCAAA AAGAGCTGTC AGTTACTGTA AGAACAAAGC TCTCACTTCC GCACACACAG 
ACCAAAGAGA TGGAAAAGCT TCTGACTGCA AACCAGAGAT GTTCCCTGGA GTTCCACAGA 
GCCCTCAAGA GACTTAAGTA CGTGTTAAGC TCCATTGCGA GGATAAAGGA AGAGCAGCAT 
GAGTCCATCA ATAAACGTGA AATGGCTTTT ATTCAGGAAG TGGAAAAGCA GAATGAACTG 
CAGATTCAAA TCCAGAGCCT TTCACAGACT TACCGTATTC CAGCCAGAGA CTTACAGATC 
AAACTGAGCC AGGAGATGGA CCTCCATATT GAGGAAATGC TCAAAGATTT TTCAGAAAAT 
GATTTCCTCA CCATAAAAAC TGAAGTCCAA CAAGTATTAA ATAATAGGAA AGAAATAACT 
GAGTTTTTGG GAAAATGGCT AAATACTCTT TTTGATACAG AAAATCTTAA GAGCACCATC 
CAGAAAGAAA ACAAAAGCAT TGGGCTCGTG AATAACTTCT ATCACAGCAG AATCACTGCC 
ATGATAAATG AATCAACGGA GTTTGAGGAA AGAAGTGCTA CCAGATCCAA AGACTTGGAT 
CAGTACCTGA AGTCACTGAA AGAAACAACT GAGCAACTGT CTGAAGTCTA CCAGACACTG 
ACCGCCTCCC AGTCTGTTGT GCATCTCCAT CCTACCGTGC AGCCTTCCAC ACGGGACAGT 
GAGCGTCCTC AGGCTGCATC AGGAGCTGAA CAACTGACCT CAAAGAACAA AATTGCTTTA 
GGAGCTGTGC CCTATAAAGA AGAAATTGAG GATCTCAAAA TGCAGCTGGT GAAGAGTGAC 
CTAGAGAAGA AGGCGACCGC CAAGGAATTC GATAAGAAAA TTCTCTCTCT AAAAGCCACT 
GTTGAACATC AAGAAGAAAT GATAAGGCTA CTGAGAGAAA ATCTTAGAGG ACATCAACAG 
GCCCAGGATA CCTCAATGAT CTCAGAACAA GACTCTCAGC TTCTGAGTAA ACCTCTAACC 
TGTGGAGGTG GCAGTGGAAT TGTACAAAGC ACAAAAGCTC TTATTTTGAA AAGTGAATAT 
AAAAGGATGG GAAGTGAGAT TTCTAAGTTA AAGCAGCAAA ATGAGCAGCT AAGAAAGCAA 
AATAATCAAC TATTAAGTGA TAACTCTCAA CTCTCCAATG AGGTTAAAAC CTGGGAGGAA 
AGAACTCTTA AAAGAGACAG TTACAGAGAA ACAACTTGTG AGAATTCTCC AAAGTCTCCT 
AAAGTGACTC GGACAGATTC TAAAAGGAGA CAGAACACAA CTTCTCAGTG CAGGGCACAA 
AATCTCCAGG ATCCTGTGCC AAAAGATTCC CCAAAATCAT GGTTTTTTGA TAACCGGTCA 
AAGTCTCTTC CAGCACCTCA CCCGATCCGC TATTTTGACA ACTCCAGTCT AGGGCTGTGC 
CCAGAACCAG ATGACGTAGA GAATGTGGAG CCTAAGACAG ACCTCTGCCA AGCCTCCTTG 
GAAAAAGATG TGTCCCAGTG CAAGACGCAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cenpe ( NM_173762.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_776123.3 Mus musculus centromere protein E (Cenpe), mRNA.

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CloneID	OMu21751
Clone ID Related Accession (Same CDS sequence)	NM_173762.4
Accession Version	NM_173762.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7416bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	centromere-associated protein E
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK133445.1, AY493378.1, CN534554.1, CN704540.1 and AC104874.11. On Oct 7, 2006 this sequence version replaced NM_173762.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164135 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGAGG AGGCTTCAGT GGCTGTCTGT GTTCGTGTGC GACCCCTCAA CAGCAGAGAA 
GAAGAACTTG GAGAAGCCAC CCACATTTAC TGGAAAACAG ATAAAAACGC TATTTATCAG 
AGCGATGGGG GTAAATCTTT CCAATTTGAT CGTGTCTTTG ACAGTAATGA AACCACGAAG 
AATGTGTATG AAGAGATTGC GGTGCCCATC ATCAGCTCCG CCATACAAGG CTACAATGGT 
ACTATATTTG CCTATGGACA AACTGCATCA GGAAAGACAC ACACGATGAT GGGTTCTGAA 
GATTGTTTGG GAGTTATACC CAGGGCAATT CATGACATTT TCCAAAGGAT AAAGAAGTTT 
CCTGAGAGAG AATTTCTCCT ACGAGTTTCT TACATGGAGA TCTACAATGA AACCATTACA 
GATTTACTCT GTAATGCTCA AAAAATGAAA CCTTTGATAA TTCGGGAAGA CACCAATAGG 
ACTGTATATG TTTCTGATCT CACAGAAGAA GTGGTTTATA CAGCAGAAAT GGCTTTGAAA 
TGGCTCGCAA CAGGAGAAAA AAACAGACAT TATGGAATAA CCAAAATGAA TCAAAGAAGC 
AGTCGTTCTC ATACGATTTT TAGGATGATT CTGGAAAGTA GAGAAAAGGC TGAGCCTTCT 
AACTGCGATG GCTCCGTCAA AGTGTCTCAT CTGAATTTGG TCGATCTTGC AGGCAGTGAA 
AGAGCTGCTC AAACAGGAGC TGAAGGTGTG CGATTGAAGG AGGGCTGTTT TATAAATCGG 
AACTTGTTTA TTTTGGGACA AGTGATTAAG AAACTCAGTG ATGGACAGGT TGGGGGTTTC 
ATAAATTACC GAGATAGCAA ATTGACACGA ATTCTTCAGA ATTCCTTGGG AGGAAATGCC 
AAGACGCGCA TCATCTGCAC GATCACCCCA GCGTCCCTGG ACGAAACCCT GACGACGCTG 
CAGTTTGCCA GCACTGCTAA GTATATGAAG AATACTCCTT ACGTTAATGA AGTGTCGAAT 
GATGAAGCTC TCTTGAAAAG ATACAGAAGA GAAATAGCGG ATCTTAGAAA ACAATTAGAG 
GAGGTAAATA CAAAGACTCG GGCACAGGAA ATGGAAAAGG ATCAACTGGC CCAGCTTCTG 
GATGAAAAAG ACTTGCTTCA GAAAGTTCAG GATGAGAAAA TTAATAACCT GAAGCGGATG 
CTGGTGACGT CATCTTCTAT TGCATTGCAG CAGGAGCTAG AGATTAAAAG GAAACGAAGA 
GTTACTTGGT GCTATGGCAA AATGAAGGAT TCAAACTATG AAAAGGAATT TAAAGTCCCG 
ACAAGCATAA CAACAAGAAA ACGTAAAACT TCTGTGACTT CTTTGAGAGA AAATTCTCTC 
ATGAAGTTCG GAGAGAGTGC AGCCTCTTCC GAGTTTGAAA TGTTGAATAA CACTCTTGAG 
TCATTGGCTG AGGTAGAGTG GAGCTCGGCA ACAACGCTCC TAAGCGAGGA AAATGTAGAA 
AGTGAGTTGA ACTCACTTAA TGCTCAATAT AATGATCTGG TATTAGACTA TGAACAGCTC 
AGAAGAGAAA ATGAAGACTT GAAATTGAAA TTAAAAGAAA AAAATGAGTT AGAGGAATTT 
GAGCTTCTAG AACAAAAAGA AGAAAGAGAT CAAGAGATGC AACTAATGCA TGAAGTTTCC 
AACTTAAAGA ATTTAATTAA ACACGCAGAA GAATATAATC AAGACCTTGA GAATGACCTA 
AGTTCAAAAG TGAAGCTTCT TAAAGAAAAA GAGGAGCAGA TTAAGAACCT GCAGGAATAC 
ATAGATGCCC AGAAGTCAGA AAAGATGAAG ATAGACCTGT CATACACATC GGACGCCACT 
GAAGACCTGA AACAGGCGAT GCGCACCCTG TCTGATTTGG ACACAGTGGC CCTTGACGCT 
AAGAAAGAGT CAGCCTTCCT TAGAAGTGAA AATCTGGAGC TGAAGGAGAA AATTAATGAA 
CTTTCGGATT CCCGTAAGCA AATGGAAAGT GACATTCAGA TGTATCAACG CCAACTGGAA 
GCAAAAAAGA AAATGCAAAC TGATCTGGAT AAGGAATTAC AGTTGGCTTT TCAAGAAATA 
TCAAAACTCA GCGCCCTGGT AGATGGCAAA GGTTTACTGT CTAATTTGGA ATTGGAGAAA 
AGGATTACTG ATCTCCAAAA AGAACTAAAT AAAGAAGCTG AAGAAAAACA AACTTTGCAA 
GAAGAAGTTA ACTTGCTGTC GGAGTTGAAA TCTTTACCGT CTGAGGTGGA AACGCTGAGG 
AGAGAGCTGT ATGAGAAATC TGAAGAGCTC CATATAATAA CCACAGAAAG AGAGAAACTG 
TTTTCTGAAA TGGCTCATAA AGACAGTAGG ATTCAAGGTT TACTTGAGGA AATTGGAAAC 
ACTAGAGATG ACCTTGCCAC TTCCCAGCTG AGTCGGAGGG GCAGTGATGG AGAATGGCAA 
GCCCTGGAAA GCCTGCACGC AGAGCTGGAG CACAGACACG CGGGGGTCCT GGAGGAGAGA 
GAGAGGCTGA AGCAGGAGAT TGGGGCTCTC TCCAAAGAAG CAGAAAGCCT GGCTTTCAGT 
TTGGATTCCG TGAAGGCCGA GCTTTCTCAC AAGACTCAAG AGCTTGAGCA GAAAACAGTT 
GAGGGTCAAG AAAGGCTAAA TAAAATGGAG GCGCTGAGAG AAGAGTTAGA AAGCAGAGAC 
TCCAGCCTGC AGTCTGTAGA GAAGGAGAAA GTCCTGCTCA CTGAGAAGCT GCAGCAAGCC 
TTGAAGGAAG TAAAAGCCTT AACCCAAGAA AAGAAAAACC TGAAGCAGCT CCAGGAGAGC 
CTGCAAACTG AGAGAGACCA ACTCAGAAGT GACATTCAGG ACACTGTGAA CATGAACATA 
GATACTCAAG AGCAGTTACT AAATGCTCTG GAGTCTCTGA AACAACACCA AGAAACAATT 
AACATGCTGA AAATGAAAGC TGCTGAGGAA CTATCCGATA ATTTGCATGT AAAGGACAGG 
GGAGGAGCTA GAGATGAAGC TCAGCAAAAG ATGGATGGCA TAGATGAACA GAATGAGAGT 
GCCCACACAT TGCTTGGTGG TGGTAAGGAC AATGAGGTAA CTGAGGAACA GAGGAAAATC 
GACTCTCTAA TGCAGGAGAA TAGCGGACTC CAACAGACAC TGGAAAGTGT TAGAGCAGAA 
AAGGAACAAC TGAAGATGGA CCTAAAGGAA AATATCGAAA TGAGTATTGA AAACCAGGAA 
GAGTTAAGAA TTCTTAGAGA TGAACTGAAA AGACAACAAG AGGTAGCTGC ACAGGAAAAG 
GATCATGCCA CCGAGAAGAC CCAGGAGCTC TCCAGGACGC AGGAAAGATT GGCAAAAACA 
GAAGAGAAGT TAGAGGAAAA GAACCAGAAA CTCCAAGAGA CACAGCAGCA ACTTCTTAGT 
ACACAAGAAG CTATGAGTAA GCTGCAGGCA AAGGTGATTG ACATGGAAAG CTTACAGAAT 
GAATTCAGGA ACCAAGGATT GGCATTGGAG CGTGTGGAGA CAGAGAAACT GGAGTTAGCT 
CAGAGACTTC ATGAAAGTTA TGAGGAGGTG AAATCTATAA CCAAAGAAAG AAATGATCTA 
AAGGAATTGC AGGAGTCATT TGAAATAGAG AAGAAGCAAC TTAAAGAATA TGCAAGGGAA 
ATAGAAGCCG CGGGCCTACA AACAAAAGAA GAACTAAACA TTGCCCACGC GAATCTAAAA 
GAGTACCAGG AAATCATCAC AGAGCTAAGA GGAAGCATTT CTGAAAACGA AGCACAAGGA 
GCAAGCACCC AGGACACGGC AAAAAGCGCC CCTGAGCTAC AAGGAGAGGT TCCAGAGCAA 
GAGCTGCTTC CTGTTGTCAA AGAAGCCAGG CACAGTGCAG AAAAAGTGAA CGGACTGGAG 
CCAGTGGGAG CACATTCCAG AACAGTGCAC TCCATGACAA TGGAGGGCAT AGAAATAGAA 
AACCTCAGGC TGACCAAGAA ACTTGAAGAA AGTCAGATGG AGATCAGCTG TCTCACTAGA 
GAGAGAGAAG ACCTGAGAAG GACACAGGAA ACACTTCAGG TCGAATGTAC CCAGCTGAAA 
GAAGATGCCA GAAGAACTTT GGCTAATCAT CTAGAAACTG AAGAGGAACT TAACCTTGCT 
CGTTGTTGCC TGAAAGAGCA GGAGAACAAG ATTGACACCC TAATAACGAG CCTCTCGCAG 
AGGGAAACTG AGCTGTCCAG TGTGCGAGGA CAACTAGCGC TGACTACTGC TGAACTGGAG 
AGAAAGGTTC AAGAACTCTG TGAGAAACAG GAAGAACTTA CTAGGAAAGA AACTAGCGAG 
GCTCAAGGGA AGATGAGTGA ACTAGAGCAA CTCAGGGAGC TGCTGCTTGC CCAGGCCTCG 
GCACTGCAGA ACGCAGAGAG TGACAGGCTG CGGCTGAATA CACAACTGGA AGAAAGTCAA 
GAGGAAATGA AAACTCTAAG GGAGGAAAGA GAAGAGCTGA GAAGAATGCA AGAGGCTCTT 
CACGTGGAAA GTGAGCAGCA GAAAGAAAGC ATGAAGGAAA TCAGCTCAAA ACTCCAAGAA 
CTACAGAACA AAGAGTATGA ATGTCTTGCA ATGAAGACTA TTAATGAGAC TCAAGGAAGC 
AGATGTGAAA TGGACCACTT GAACCAGCAG CTGGAGGCCC AGAAGTCAAC TCTGGAAAAG 
GTGGAGATGG AGAATGTAAA CTTGACTCAA AGACTTCATG AGACCCTTGA GGAAATGAGA 
TCTGTAGCAA AAGAAAGAGA TGAGCTTTGG AGCATGGAGG AGCGCCTCAC AGTAGAGAGA 
GACCAACTGA AGAAGAGCCT TGAAGAAACA GTAACTAAAG GTATGGAAAA AGAGGAAGAG 
CTAAGAGTTG CGCATGTGCA TCTGGAAGAG CACCAGGAAA CTATCAACAA GCTCAGAAAG 
ATGGTGTCTG ACTACACAGA TGAAATATCA CATACTCAAG GGGACCTAAA GCACACAAAT 
GCCGTTGTAG AAGCTCAGAA CCAAGATCTC CGGGAAAAAG AACATCAACT TTCTCAAGTA 
AAAGCTGATC TCAGAGAAAC CGTGGATCAA ATGGAGCAAC TGAAGAAGAA GCTCGAAGCC 
CAGAGTTCGA CTCTAGAAAG CAGAGAAATA GAGAAGTTAG AATTGACTCA GCAACTTAAC 
GAAAACCTTA AAAAAATAAC ACTTGTCACT AAGGAAAATG ATAGCCTAAA AATTATGGAT 
GAAGCCCTCA GGGAAGAACG AGACCAGCTA CGGAAAAGTC TTCAGCAAAC AGAGGCAAGG 
GATCTGGAAA ATCAAGAGAA ACTAAGAATT GCTCACATGA ATCTAAAAGA GCACCAAGAA 
ACTATTGACC GACTCATGGA GACTATGTCT GAGAAGACAG AGGAAATATC AAATATGAAA 
ATGGAGTTAG AAAATGTAAA CATGAAATTA CAAGAAAAGG TTCAAGAACT TAAGACAAGT 
GAGCGTCAAC GTGTTAAGTT AAAAGCAGAT GCCAGTGAAG CCAAGAAGGA GCTCAAGGAG 
CAGGGCTTAA CTCTGAGTAA GATAGAGATG GAGAACTTAA ATCTCGCTCA GAAGATTCAT 
GAGAACCTTG AAGAAATGAA ATCTGTAAGA AAAGAAAGAG ATGATCTAAA GAAACTTGAG 
GAGATACTCA GAATGGAGAG AGACCAGCTC AAGGACAACC TGAGAGAAGC CATGCTTAAG 
GCCCACCAGA ACCATGAAGA GACAATGAAA TGTGGAAAGG GGTTACTGTG TGCTGGAGAA 
TACTGTACAG GAAGACTTCG AGAAAAGTGC TTCAGGATAG AAAAACTCCT GAAAAGATAC 
TCAGAAATGG CTAATGATTA TGAGTGCTTG AATAAAGTCT CTCTTGACTT GGAGAGAGAA 
ACTAAAACCC AAAAAGAGCT GTCAGTTACT GTAAGAACAA AGCTCTCACT TCCGCACACA 
CAGACCAAAG AGATGGAAAA GCTTCTGACT GCAAACCAGA GATGTTCCCT GGAGTTCCAC 
AGAGCCCTCA AGAGACTTAA GTACGTGTTA AGCTCCATTG CGAGGATAAA GGAAGAGCAG 
CATGAGTCCA TCAATAAACG TGAAATGGCT TTTATTCAGG AAGTGGAAAA GCAGAATGAA 
CTGCAGATTC AAATCCAGAG CCTTTCACAG ACTTACCGTA TTCCAGCCAG AGACTTACAG 
ATCAAACTGA GCCAGGAGAT GGACCTCCAT ATTGAGGAAA TGCTCAAAGA TTTTTCAGAA 
AATGATTTCC TCACCATAAA AACTGAAGTC CAACAAGTAT TAAATAATAG GAAAGAAATA 
ACTGAGTTTT TGGGAAAATG GCTAAATACT CTTTTTGATA CAGAAAATCT TAAGAGCACC 
ATCCAGAAAG AAAACAAAAG CATTGGGCTC GTGAATAACT TCTATCACAG CAGAATCACT 
GCCATGATAA ATGAATCAAC GGAGTTTGAG GAAAGAAGTG CTACCAGATC CAAAGACTTG 
GATCAGTACC TGAAGTCACT GAAAGAAACA ACTGAGCAAC TGTCTGAAGT CTACCAGACA 
CTGACCGCCT CCCAGTCTGT TGTGCATCTC CATCCTACCG TGCAGCCTTC CACACGGGAC 
AGTGAGCGTC CTCAGGCTGC ATCAGGAGCT GAACAACTGA CCTCAAAGAA CAAAATTGCT 
TTAGGAGCTG TGCCCTATAA AGAAGAAATT GAGGATCTCA AAATGCAGCT GGTGAAGAGT 
GACCTAGAGA AGAAGGCGAC CGCCAAGGAA TTCGATAAGA AAATTCTCTC TCTAAAAGCC 
ACTGTTGAAC ATCAAGAAGA AATGATAAGG CTACTGAGAG AAAATCTTAG AGGACATCAA 
CAGGCCCAGG ATACCTCAAT GATCTCAGAA CAAGACTCTC AGCTTCTGAG TAAACCTCTA 
ACCTGTGGAG GTGGCAGTGG AATTGTACAA AGCACAAAAG CTCTTATTTT GAAAAGTGAA 
TATAAAAGGA TGGGAAGTGA GATTTCTAAG TTAAAGCAGC AAAATGAGCA GCTAAGAAAG 
CAAAATAATC AACTATTAAG TGATAACTCT CAACTCTCCA ATGAGGTTAA AACCTGGGAG 
GAAAGAACTC TTAAAAGAGA CAGTTACAGA GAAACAACTT GTGAGAATTC TCCAAAGTCT 
CCTAAAGTGA CTCGGACAGA TTCTAAAAGG AGACAGAACA CAACTTCTCA GTGCAGGGCA 
CAAAATCTCC AGGATCCTGT GCCAAAAGAT TCCCCAAAAT CATGGTTTTT TGATAACCGG 
TCAAAGTCTC TTCCAGCACC TCACCCGATC CGCTATTTTG ACAACTCCAG TCTAGGGCTG 
TGCCCAGAAC CAGATGACGT AGAGAATGTG GAGCCTAAGA CAGACCTCTG CCAAGCCTCC 
TTGGAAAAAG ATGTGTCCCA GTGCAAGACG CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776123.3
CDS	97..7512
Translation	MAEEASVAVCVRVRPLNSREEELGEATHIYWKTDKNAIYQSDGGKSFQFDRVFDSNETTKNVYEEIAVPIISSAIQGYNGTIFAYGQTASGKTHTMMGSEDCLGVIPRAIHDIFQRIKKFPEREFLLRVSYMEIYNETITDLLCNAQKMKPLIIREDTNRTVYVSDLTEEVVYTAEMALKWLATGEKNRHYGITKMNQRSSRSHTIFRMILESREKAEPSNCDGSVKVSHLNLVDLAGSERAAQTGAEGVRLKEGCFINRNLFILGQVIKKLSDGQVGGFINYRDSKLTRILQNSLGGNAKTRIICTITPASLDETLTTLQFASTAKYMKNTPYVNEVSNDEALLKRYRREIADLRKQLEEVNTKTRAQEMEKDQLAQLLDEKDLLQKVQDEKINNLKRMLVTSSSIALQQELEIKRKRRVTWCYGKMKDSNYEKEFKVPTSITTRKRKTSVTSLRENSLMKFGESAASSEFEMLNNTLESLAEVEWSSATTLLSEENVESELNSLNAQYNDLVLDYEQLRRENEDLKLKLKEKNELEEFELLEQKEERDQEMQLMHEVSNLKNLIKHAEEYNQDLENDLSSKVKLLKEKEEQIKNLQEYIDAQKSEKMKIDLSYTSDATEDLKQAMRTLSDLDTVALDAKKESAFLRSENLELKEKINELSDSRKQMESDIQMYQRQLEAKKKMQTDLDKELQLAFQEISKLSALVDGKGLLSNLELEKRITDLQKELNKEAEEKQTLQEEVNLLSELKSLPSEVETLRRELYEKSEELHIITTEREKLFSEMAHKDSRIQGLLEEIGNTRDDLATSQLSRRGSDGEWQALESLHAELEHRHAGVLEERERLKQEIGALSKEAESLAFSLDSVKAELSHKTQELEQKTVEGQERLNKMEALREELESRDSSLQSVEKEKVLLTEKLQQALKEVKALTQEKKNLKQLQESLQTERDQLRSDIQDTVNMNIDTQEQLLNALESLKQHQETINMLKMKAAEELSDNLHVKDRGGARDEAQQKMDGIDEQNESAHTLLGGGKDNEVTEEQRKIDSLMQENSGLQQTLESVRAEKEQLKMDLKENIEMSIENQEELRILRDELKRQQEVAAQEKDHATEKTQELSRTQERLAKTEEKLEEKNQKLQETQQQLLSTQEAMSKLQAKVIDMESLQNEFRNQGLALERVETEKLELAQRLHESYEEVKSITKERNDLKELQESFEIEKKQLKEYAREIEAAGLQTKEELNIAHANLKEYQEIITELRGSISENEAQGASTQDTAKSAPELQGEVPEQELLPVVKEARHSAEKVNGLEPVGAHSRTVHSMTMEGIEIENLRLTKKLEESQMEISCLTREREDLRRTQETLQVECTQLKEDARRTLANHLETEEELNLARCCLKEQENKIDTLITSLSQRETELSSVRGQLALTTAELERKVQELCEKQEELTRKETSEAQGKMSELEQLRELLLAQASALQNAESDRLRLNTQLEESQEEMKTLREEREELRRMQEALHVESEQQKESMKEISSKLQELQNKEYECLAMKTINETQGSRCEMDHLNQQLEAQKSTLEKVEMENVNLTQRLHETLEEMRSVAKERDELWSMEERLTVERDQLKKSLEETVTKGMEKEEELRVAHVHLEEHQETINKLRKMVSDYTDEISHTQGDLKHTNAVVEAQNQDLREKEHQLSQVKADLRETVDQMEQLKKKLEAQSSTLESREIEKLELTQQLNENLKKITLVTKENDSLKIMDEALREERDQLRKSLQQTEARDLENQEKLRIAHMNLKEHQETIDRLMETMSEKTEEISNMKMELENVNMKLQEKVQELKTSERQRVKLKADASEAKKELKEQGLTLSKIEMENLNLAQKIHENLEEMKSVRKERDDLKKLEEILRMERDQLKDNLREAMLKAHQNHEETMKCGKGLLCAGEYCTGRLREKCFRIEKLLKRYSEMANDYECLNKVSLDLERETKTQKELSVTVRTKLSLPHTQTKEMEKLLTANQRCSLEFHRALKRLKYVLSSIARIKEEQHESINKREMAFIQEVEKQNELQIQIQSLSQTYRIPARDLQIKLSQEMDLHIEEMLKDFSENDFLTIKTEVQQVLNNRKEITEFLGKWLNTLFDTENLKSTIQKENKSIGLVNNFYHSRITAMINESTEFEERSATRSKDLDQYLKSLKETTEQLSEVYQTLTASQSVVHLHPTVQPSTRDSERPQAASGAEQLTSKNKIALGAVPYKEEIEDLKMQLVKSDLEKKATAKEFDKKILSLKATVEHQEEMIRLLRENLRGHQQAQDTSMISEQDSQLLSKPLTCGGGSGIVQSTKALILKSEYKRMGSEISKLKQQNEQLRKQNNQLLSDNSQLSNEVKTWEERTLKRDSYRETTCENSPKSPKVTRTDSKRRQNTTSQCRAQNLQDPVPKDSPKSWFFDNRSKSLPAPHPIRYFDNSSLGLCPEPDDVENVEPKTDLCQASLEKDVSQCKTQ

Target ORF information:

RefSeq Version	NM_173762.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus centromere protein E (Cenpe), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173762.4

ORF Insert Sequence:

ATGGCCGAGG AGGCTTCAGT GGCTGTCTGT GTTCGTGTGC GACCCCTCAA CAGCAGAGAA 
GAAGAACTTG GAGAAGCCAC CCACATTTAC TGGAAAACAG ATAAAAACGC TATTTATCAG 
AGCGATGGGG GTAAATCTTT CCAATTTGAT CGTGTCTTTG ACAGTAATGA AACCACGAAG 
AATGTGTATG AAGAGATTGC GGTGCCCATC ATCAGCTCCG CCATACAAGG CTACAATGGT 
ACTATATTTG CCTATGGACA AACTGCATCA GGAAAGACAC ACACGATGAT GGGTTCTGAA 
GATTGTTTGG GAGTTATACC CAGGGCAATT CATGACATTT TCCAAAGGAT AAAGAAGTTT 
CCTGAGAGAG AATTTCTCCT ACGAGTTTCT TACATGGAGA TCTACAATGA AACCATTACA 
GATTTACTCT GTAATGCTCA AAAAATGAAA CCTTTGATAA TTCGGGAAGA CACCAATAGG 
ACTGTATATG TTTCTGATCT CACAGAAGAA GTGGTTTATA CAGCAGAAAT GGCTTTGAAA 
TGGCTCGCAA CAGGAGAAAA AAACAGACAT TATGGAATAA CCAAAATGAA TCAAAGAAGC 
AGTCGTTCTC ATACGATTTT TAGGATGATT CTGGAAAGTA GAGAAAAGGC TGAGCCTTCT 
AACTGCGATG GCTCCGTCAA AGTGTCTCAT CTGAATTTGG TCGATCTTGC AGGCAGTGAA 
AGAGCTGCTC AAACAGGAGC TGAAGGTGTG CGATTGAAGG AGGGCTGTTT TATAAATCGG 
AACTTGTTTA TTTTGGGACA AGTGATTAAG AAACTCAGTG ATGGACAGGT TGGGGGTTTC 
ATAAATTACC GAGATAGCAA ATTGACACGA ATTCTTCAGA ATTCCTTGGG AGGAAATGCC 
AAGACGCGCA TCATCTGCAC GATCACCCCA GCGTCCCTGG ACGAAACCCT GACGACGCTG 
CAGTTTGCCA GCACTGCTAA GTATATGAAG AATACTCCTT ACGTTAATGA AGTGTCGAAT 
GATGAAGCTC TCTTGAAAAG ATACAGAAGA GAAATAGCGG ATCTTAGAAA ACAATTAGAG 
GAGGTAAATA CAAAGACTCG GGCACAGGAA ATGGAAAAGG ATCAACTGGC CCAGCTTCTG 
GATGAAAAAG ACTTGCTTCA GAAAGTTCAG GATGAGAAAA TTAATAACCT GAAGCGGATG 
CTGGTGACGT CATCTTCTAT TGCATTGCAG CAGGAGCTAG AGATTAAAAG GAAACGAAGA 
GTTACTTGGT GCTATGGCAA AATGAAGGAT TCAAACTATG AAAAGGAATT TAAAGTCCCG 
ACAAGCATAA CAACAAGAAA ACGTAAAACT TCTGTGACTT CTTTGAGAGA AAATTCTCTC 
ATGAAGTTCG GAGAGAGTGC AGCCTCTTCC GAGTTTGAAA TGTTGAATAA CACTCTTGAG 
TCATTGGCTG AGGTAGAGTG GAGCTCGGCA ACAACGCTCC TAAGCGAGGA AAATGTAGAA 
AGTGAGTTGA ACTCACTTAA TGCTCAATAT AATGATCTGG TATTAGACTA TGAACAGCTC 
AGAAGAGAAA ATGAAGACTT GAAATTGAAA TTAAAAGAAA AAAATGAGTT AGAGGAATTT 
GAGCTTCTAG AACAAAAAGA AGAAAGAGAT CAAGAGATGC AACTAATGCA TGAAGTTTCC 
AACTTAAAGA ATTTAATTAA ACACGCAGAA GAATATAATC AAGACCTTGA GAATGACCTA 
AGTTCAAAAG TGAAGCTTCT TAAAGAAAAA GAGGAGCAGA TTAAGAACCT GCAGGAATAC 
ATAGATGCCC AGAAGTCAGA AAAGATGAAG ATAGACCTGT CATACACATC GGACGCCACT 
GAAGACCTGA AACAGGCGAT GCGCACCCTG TCTGATTTGG ACACAGTGGC CCTTGACGCT 
AAGAAAGAGT CAGCCTTCCT TAGAAGTGAA AATCTGGAGC TGAAGGAGAA AATTAATGAA 
CTTTCGGATT CCCGTAAGCA AATGGAAAGT GACATTCAGA TGTATCAACG CCAACTGGAA 
GCAAAAAAGA AAATGCAAAC TGATCTGGAT AAGGAATTAC AGTTGGCTTT TCAAGAAATA 
TCAAAACTCA GCGCCCTGGT AGATGGCAAA GGTTTACTGT CTAATTTGGA ATTGGAGAAA 
AGGATTACTG ATCTCCAAAA AGAACTAAAT AAAGAAGCTG AAGAAAAACA AACTTTGCAA 
GAAGAAGTTA ACTTGCTGTC GGAGTTGAAA TCTTTACCGT CTGAGGTGGA AACGCTGAGG 
AGAGAGCTGT ATGAGAAATC TGAAGAGCTC CATATAATAA CCACAGAAAG AGAGAAACTG 
TTTTCTGAAA TGGCTCATAA AGACAGTAGG ATTCAAGGTT TACTTGAGGA AATTGGAAAC 
ACTAGAGATG ACCTTGCCAC TTCCCAGCTG AGTCGGAGGG GCAGTGATGG AGAATGGCAA 
GCCCTGGAAA GCCTGCACGC AGAGCTGGAG CACAGACACG CGGGGGTCCT GGAGGAGAGA 
GAGAGGCTGA AGCAGGAGAT TGGGGCTCTC TCCAAAGAAG CAGAAAGCCT GGCTTTCAGT 
TTGGATTCCG TGAAGGCCGA GCTTTCTCAC AAGACTCAAG AGCTTGAGCA GAAAACAGTT 
GAGGGTCAAG AAAGGCTAAA TAAAATGGAG GCGCTGAGAG AAGAGTTAGA AAGCAGAGAC 
TCCAGCCTGC AGTCTGTAGA GAAGGAGAAA GTCCTGCTCA CTGAGAAGCT GCAGCAAGCC 
TTGAAGGAAG TAAAAGCCTT AACCCAAGAA AAGAAAAACC TGAAGCAGCT CCAGGAGAGC 
CTGCAAACTG AGAGAGACCA ACTCAGAAGT GACATTCAGG ACACTGTGAA CATGAACATA 
GATACTCAAG AGCAGTTACT AAATGCTCTG GAGTCTCTGA AACAACACCA AGAAACAATT 
AACATGCTGA AAATGAAAGC TGCTGAGGAA CTATCCGATA ATTTGCATGT AAAGGACAGG 
GGAGGAGCTA GAGATGAAGC TCAGCAAAAG ATGGATGGCA TAGATGAACA GAATGAGAGT 
GCCCACACAT TGCTTGGTGG TGGTAAGGAC AATGAGGTAA CTGAGGAACA GAGGAAAATC 
GACTCTCTAA TGCAGGAGAA TAGCGGACTC CAACAGACAC TGGAAAGTGT TAGAGCAGAA 
AAGGAACAAC TGAAGATGGA CCTAAAGGAA AATATCGAAA TGAGTATTGA AAACCAGGAA 
GAGTTAAGAA TTCTTAGAGA TGAACTGAAA AGACAACAAG AGGTAGCTGC ACAGGAAAAG 
GATCATGCCA CCGAGAAGAC CCAGGAGCTC TCCAGGACGC AGGAAAGATT GGCAAAAACA 
GAAGAGAAGT TAGAGGAAAA GAACCAGAAA CTCCAAGAGA CACAGCAGCA ACTTCTTAGT 
ACACAAGAAG CTATGAGTAA GCTGCAGGCA AAGGTGATTG ACATGGAAAG CTTACAGAAT 
GAATTCAGGA ACCAAGGATT GGCATTGGAG CGTGTGGAGA CAGAGAAACT GGAGTTAGCT 
CAGAGACTTC ATGAAAGTTA TGAGGAGGTG AAATCTATAA CCAAAGAAAG AAATGATCTA 
AAGGAATTGC AGGAGTCATT TGAAATAGAG AAGAAGCAAC TTAAAGAATA TGCAAGGGAA 
ATAGAAGCCG CGGGCCTACA AACAAAAGAA GAACTAAACA TTGCCCACGC GAATCTAAAA 
GAGTACCAGG AAATCATCAC AGAGCTAAGA GGAAGCATTT CTGAAAACGA AGCACAAGGA 
GCAAGCACCC AGGACACGGC AAAAAGCGCC CCTGAGCTAC AAGGAGAGGT TCCAGAGCAA 
GAGCTGCTTC CTGTTGTCAA AGAAGCCAGG CACAGTGCAG AAAAAGTGAA CGGACTGGAG 
CCAGTGGGAG CACATTCCAG AACAGTGCAC TCCATGACAA TGGAGGGCAT AGAAATAGAA 
AACCTCAGGC TGACCAAGAA ACTTGAAGAA AGTCAGATGG AGATCAGCTG TCTCACTAGA 
GAGAGAGAAG ACCTGAGAAG GACACAGGAA ACACTTCAGG TCGAATGTAC CCAGCTGAAA 
GAAGATGCCA GAAGAACTTT GGCTAATCAT CTAGAAACTG AAGAGGAACT TAACCTTGCT 
CGTTGTTGCC TGAAAGAGCA GGAGAACAAG ATTGACACCC TAATAACGAG CCTCTCGCAG 
AGGGAAACTG AGCTGTCCAG TGTGCGAGGA CAACTAGCGC TGACTACTGC TGAACTGGAG 
AGAAAGGTTC AAGAACTCTG TGAGAAACAG GAAGAACTTA CTAGGAAAGA AACTAGCGAG 
GCTCAAGGGA AGATGAGTGA ACTAGAGCAA CTCAGGGAGC TGCTGCTTGC CCAGGCCTCG 
GCACTGCAGA ACGCAGAGAG TGACAGGCTG CGGCTGAATA CACAACTGGA AGAAAGTCAA 
GAGGAAATGA AAACTCTAAG GGAGGAAAGA GAAGAGCTGA GAAGAATGCA AGAGGCTCTT 
CACGTGGAAA GTGAGCAGCA GAAAGAAAGC ATGAAGGAAA TCAGCTCAAA ACTCCAAGAA 
CTACAGAACA AAGAGTATGA ATGTCTTGCA ATGAAGACTA TTAATGAGAC TCAAGGAAGC 
AGATGTGAAA TGGACCACTT GAACCAGCAG CTGGAGGCCC AGAAGTCAAC TCTGGAAAAG 
GTGGAGATGG AGAATGTAAA CTTGACTCAA AGACTTCATG AGACCCTTGA GGAAATGAGA 
TCTGTAGCAA AAGAAAGAGA TGAGCTTTGG AGCATGGAGG AGCGCCTCAC AGTAGAGAGA 
GACCAACTGA AGAAGAGCCT TGAAGAAACA GTAACTAAAG GTATGGAAAA AGAGGAAGAG 
CTAAGAGTTG CGCATGTGCA TCTGGAAGAG CACCAGGAAA CTATCAACAA GCTCAGAAAG 
ATGGTGTCTG ACTACACAGA TGAAATATCA CATACTCAAG GGGACCTAAA GCACACAAAT 
GCCGTTGTAG AAGCTCAGAA CCAAGATCTC CGGGAAAAAG AACATCAACT TTCTCAAGTA 
AAAGCTGATC TCAGAGAAAC CGTGGATCAA ATGGAGCAAC TGAAGAAGAA GCTCGAAGCC 
CAGAGTTCGA CTCTAGAAAG CAGAGAAATA GAGAAGTTAG AATTGACTCA GCAACTTAAC 
GAAAACCTTA AAAAAATAAC ACTTGTCACT AAGGAAAATG ATAGCCTAAA AATTATGGAT 
GAAGCCCTCA GGGAAGAACG AGACCAGCTA CGGAAAAGTC TTCAGCAAAC AGAGGCAAGG 
GATCTGGAAA ATCAAGAGAA ACTAAGAATT GCTCACATGA ATCTAAAAGA GCACCAAGAA 
ACTATTGACC GACTCATGGA GACTATGTCT GAGAAGACAG AGGAAATATC AAATATGAAA 
ATGGAGTTAG AAAATGTAAA CATGAAATTA CAAGAAAAGG TTCAAGAACT TAAGACAAGT 
GAGCGTCAAC GTGTTAAGTT AAAAGCAGAT GCCAGTGAAG CCAAGAAGGA GCTCAAGGAG 
CAGGGCTTAA CTCTGAGTAA GATAGAGATG GAGAACTTAA ATCTCGCTCA GAAGATTCAT 
GAGAACCTTG AAGAAATGAA ATCTGTAAGA AAAGAAAGAG ATGATCTAAA GAAACTTGAG 
GAGATACTCA GAATGGAGAG AGACCAGCTC AAGGACAACC TGAGAGAAGC CATGCTTAAG 
GCCCACCAGA ACCATGAAGA GACAATGAAA TGTGGAAAGG GGTTACTGTG TGCTGGAGAA 
TACTGTACAG GAAGACTTCG AGAAAAGTGC TTCAGGATAG AAAAACTCCT GAAAAGATAC 
TCAGAAATGG CTAATGATTA TGAGTGCTTG AATAAAGTCT CTCTTGACTT GGAGAGAGAA 
ACTAAAACCC AAAAAGAGCT GTCAGTTACT GTAAGAACAA AGCTCTCACT TCCGCACACA 
CAGACCAAAG AGATGGAAAA GCTTCTGACT GCAAACCAGA GATGTTCCCT GGAGTTCCAC 
AGAGCCCTCA AGAGACTTAA GTACGTGTTA AGCTCCATTG CGAGGATAAA GGAAGAGCAG 
CATGAGTCCA TCAATAAACG TGAAATGGCT TTTATTCAGG AAGTGGAAAA GCAGAATGAA 
CTGCAGATTC AAATCCAGAG CCTTTCACAG ACTTACCGTA TTCCAGCCAG AGACTTACAG 
ATCAAACTGA GCCAGGAGAT GGACCTCCAT ATTGAGGAAA TGCTCAAAGA TTTTTCAGAA 
AATGATTTCC TCACCATAAA AACTGAAGTC CAACAAGTAT TAAATAATAG GAAAGAAATA 
ACTGAGTTTT TGGGAAAATG GCTAAATACT CTTTTTGATA CAGAAAATCT TAAGAGCACC 
ATCCAGAAAG AAAACAAAAG CATTGGGCTC GTGAATAACT TCTATCACAG CAGAATCACT 
GCCATGATAA ATGAATCAAC GGAGTTTGAG GAAAGAAGTG CTACCAGATC CAAAGACTTG 
GATCAGTACC TGAAGTCACT GAAAGAAACA ACTGAGCAAC TGTCTGAAGT CTACCAGACA 
CTGACCGCCT CCCAGTCTGT TGTGCATCTC CATCCTACCG TGCAGCCTTC CACACGGGAC 
AGTGAGCGTC CTCAGGCTGC ATCAGGAGCT GAACAACTGA CCTCAAAGAA CAAAATTGCT 
TTAGGAGCTG TGCCCTATAA AGAAGAAATT GAGGATCTCA AAATGCAGCT GGTGAAGAGT 
GACCTAGAGA AGAAGGCGAC CGCCAAGGAA TTCGATAAGA AAATTCTCTC TCTAAAAGCC 
ACTGTTGAAC ATCAAGAAGA AATGATAAGG CTACTGAGAG AAAATCTTAG AGGACATCAA 
CAGGCCCAGG ATACCTCAAT GATCTCAGAA CAAGACTCTC AGCTTCTGAG TAAACCTCTA 
ACCTGTGGAG GTGGCAGTGG AATTGTACAA AGCACAAAAG CTCTTATTTT GAAAAGTGAA 
TATAAAAGGA TGGGAAGTGA GATTTCTAAG TTAAAGCAGC AAAATGAGCA GCTAAGAAAG 
CAAAATAATC AACTATTAAG TGATAACTCT CAACTCTCCA ATGAGGTTAA AACCTGGGAG 
GAAAGAACTC TTAAAAGAGA CAGTTACAGA GAAACAACTT GTGAGAATTC TCCAAAGTCT 
CCTAAAGTGA CTCGGACAGA TTCTAAAAGG AGACAGAACA CAACTTCTCA GTGCAGGGCA 
CAAAATCTCC AGGATCCTGT GCCAAAAGAT TCCCCAAAAT CATGGTTTTT TGATAACCGG 
TCAAAGTCTC TTCCAGCACC TCACCCGATC CGCTATTTTG ACAACTCCAG TCTAGGGCTG 
TGCCCAGAAC CAGATGACGT AGAGAATGTG GAGCCTAAGA CAGACCTCTG CCAAGCCTCC 
TTGGAAAAAG ATGTGTCCCA GTGCAAGACG CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

The Interleukin-2-mTORc1 Kinase Axis Defines the Signaling, Differentiation, and Metabolism of T Helper 1 and Follicular B Helper T Cells.
Immunity43(4)690-702(2015 Oct)
Ray JP,Staron MM,Shyer JA,Ho PC,Marshall HD,Gray SM,Laidlaw BJ,Araki K,Ahmed R,Kaech SM,Craft J

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

Conserved molecular signatures of neurogenesis in the hippocampal subgranular zone of rodents and primates.
Development (Cambridge, England)140(22)4633-44(2013 Nov)
Miller JA,Nathanson J,Franjic D,Shim S,Dalley RA,Shapouri S,Smith KA,Sunkin SM,Bernard A,Bennett JL,Lee CK,Hawrylycz MJ,Jones AR,Amaral DG,?estan N,Gage FH,Lein ES

Chromosome missegregation rate predicts whether aneuploidy will promote or suppress tumors.
Proceedings of the National Academy of Sciences of the United States of America110(44)E4134-41(2013 Oct)
Silk AD,Zasadil LM,Holland AJ,Vitre B,Cleveland DW,Weaver BA

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Cenpe cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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