Pias3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pias3
Entrez Gene ID	229615
Full Name	protein inhibitor of activated STAT 3
Synonyms	Pias3l
General protein information	Preferred Names protein inhibitor of activated STAT 3 Names E3 SUMO-protein ligase PIAS3 protein inhibitor of activated STAT protein 3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3\|3 F2.1

mRNA and Protein(s)

mRNA	Protein	Name
NM_018812.2	NP_061282.2	E3 SUMO-protein ligase PIAS3 isoform 2
XM_006501393.1	XP_006501456.1	E3 SUMO-protein ligase PIAS3 isoform X1
NM_146135.2	NP_666247.1	E3 SUMO-protein ligase PIAS3 isoform 1
XM_030252586.1	XP_030108446.1	E3 SUMO-protein ligase PIAS3 isoform X2
NM_001165949.1	NP_001159421.1	E3 SUMO-protein ligase PIAS3 isoform 3

Pathways from BioSystems

Homologies

Bos taurus (cattle)	PIAS3	NP_001095655.1
Mus musculus (house mouse)	Pias3	NP_001159421.1
Pan troglodytes (chimpanzee)	PIAS3	XP_003949540.1
	PIAS3	XP_001095153.2
Canis lupus familiaris (dog)	PIAS3	XP_859310.2
Rattus norvegicus (Norway rat)	Pias3	NP_113972.2
Homo sapiens (human)	PIAS3	NP_006090.2
Xenopus tropicalis (tropical clawed frog)	pias3	NP_001116890.1

Gene Ontology
Bibliography

Related articles in PubMed

Foxp1 regulation of neonatal vocalizations via cortical development.
Usui N, Araujo DJ, Kulkarni A, Co M, Ellegood J, Harper M, Toriumi K, Lerch JP, Konopka G
Genes & development31(20)2039-2055(2017 Oct)

Pias3 is necessary for dorso-ventral patterning and visual response of retinal cones but is not required for rod photoreceptor differentiation.
Campla CK, Breit H, Dong L, Gumerson JD, Roger JE, Swaroop A
Biology open6(6)881-890(2017 Jun)

Protein Inhibitor of Activated STAT3 Suppresses Oxidized LDL-induced Cell Responses during Atherosclerosis in Apolipoprotein E-deficient Mice.
Wang R, Zhang Y, Xu L, Lin Y, Yang X, Bai L, Chen Y, Zhao S, Fan J, Cheng X, Liu E
Scientific reports636790(2016 Nov)

Transcriptional activity of erythroid Kruppel-like factor (EKLF/KLF1) modulated by PIAS3 (protein inhibitor of activated STAT3).
Siatecka M, Soni S, Planutis A, Bieker JJ
The Journal of biological chemistry290(15)9929-40(2015 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that PIAS3 (protein inhibitor of activated STAT3) interaction modulates EKLF (erythroid Kruppel-like factor) activity in a promoter-dependent and SUMO-independent manner.
Title: Transcriptional activity of erythroid Kruppel-like factor (EKLF/KLF1) modulated by PIAS3 (protein inhibitor of activated STAT3).

PIAS3 suppresses acute graft-versus-host disease by modulating effector T and B cell subsets through inhibition of STAT3 activation.
Title: PIAS3 suppresses acute graft-versus-host disease by modulating effector T and B cell subsets through inhibition of STAT3 activation.

These data indicate that tachypacing decreased ATBF1, leading to enhanced STAT3 DNA-binding activity due to the reduced formation of a binary complex of ATBF1 and PIAS3.
Title: Down-regulation of ATBF1 activates STAT3 signaling via PIAS3 in pacing-induced HL-1 atrial myocytes.

MRL/lpr mice have significantly increased expressions of STAT3 mRNA and protein and decreased expression of mRNA PIAS3 in the kidneys compared with BALB/C mice.
Title: [Expression of STAT3 and PIAS3 in renal tissues of MRL/lpr mice].

L97A, R99N and R99Q mutations of the PINIT domain (PIAS3(85-272) ) were found to abrogate binding to STAT3, suggesting that these residues were part of a potential binding surface.
Title: The PINIT domain of PIAS3: structure-function analysis of its interaction with STAT3.

The following Pias3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pias3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu10144	NM_001165949.1 Latest version!	Mus musculus protein inhibitor of activated STAT 3 (Pias3), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$370.30 $529.00
OMu10987	NM_018812.2 Latest version!	Mus musculus protein inhibitor of activated STAT 3 (Pias3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu12254	NM_146135.2 Latest version!	Mus musculus protein inhibitor of activated STAT 3 (Pias3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu12254	XM_006501393.1 Latest version!	Mus musculus protein inhibitor of activated STAT 3 (Pias3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu88219	XM_030252586.1 Latest version!	Mus musculus protein inhibitor of activated STAT 3 (Pias3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pias3 ( NM_001165949.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001159421.1 Mus musculus protein inhibitor of activated STAT 3 (Pias3), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu10144
Clone ID Related Accession (Same CDS sequence)	NM_001165949.1
Accession Version	NM_001165949.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1887bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-15
Organism	Mus musculus(house mouse)
Product	E3 SUMO-protein ligase PIAS3 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY154092.1, BC051252.1, BE989927.1 and BB042656.1. Transcript Variant: This variant (3) differs in the 5' UTR and 5' coding region, and uses an alternate translational start codon, compared to variant 1. The encoded isoform (3) has a distinct N-terminus and is longer than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC051252.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGAGC TGGGCGAGTT AAAGCACATG GTGATGAGTT TCCGAGTGTC TGAGCTCCAG 
GTGCTCCTCG GCTTCGCTGG CAGGAACAAG AGTGGGCGGA AACACGAGCT GCTGGCCAAG 
GCCCTGCACC TCCTCAAGTC TAGCTGCGCC CCCAGCGTCC AGATGAAGAT CAAAGAACTT 
TATCGCAGGC GCTTTCCCCG GAAGACCCTG GGGCCTTCTG ATCTCTCCTT GCTTTCTTTG 
CCCCCTGGCA CCTCTCCTGT AGGCTCCCCC GGCCCCCTTG CTCCCATCCC TCCCACCCTT 
CTGACCCCTG GCACCTTGCT AGGCCCTAAG CGTGAGGTGG ACATGCATCC TCCTCTGCCG 
CAGCCTGTGC ACCCCGATGT CACCATGAAG CCACTGCCCT TCTATGAAGT CTATGGGGAG 
CTCATCCGAC CCACCACCCT TGCGTCCACC TCCAGCCAGA GGTTCGAGGA AGCCCACTTC 
ACCTTCGCGC TCACTCCCCA GCAGCTGCAG CAGATTCTCA CGTCCAGGGA GGTTCTGCCA 
GGAGCCAAGT GTGATTACAC CATACAAGTG CAGCTCAGAT TCTGTCTCTG TGAGACCAGC 
TGCCCTCAGG AGGACTATTT CCCCCCTAAC CTCTTTGTTA AGGTTAATGG GAAACTCTGC 
CCCCTGCCGG GTTACCTCCC TCCAACCAAG AATGGAGCTG AGCCCAAGAG GCCCAGCCGT 
CCGATCAACA TCACACCCTT GGCTCGACTC TCAGCCACTG TCCCCAACAC CATCGTAGTT 
AATTGGTCAT CTGAGTTTGG ACGGAATTAC TCCTTGTCCG TGTACCTGGT GAGGCAATTG 
ACTGCAGGGA CCCTTCTACA AAAACTCAGA GCCAAGGGGA TCCGGAATCC AGACCATTCC 
CGGGCACTGA TCAAGGAGAA ACTGACTGCT GACCCCGACA GTGAAGTGGC TACTACAAGT 
CTCCGGGTGT CACTCATGTG CCCGCTAGGG AAGATGCGCC TGACTGTCCC GTGTCGTGCC 
CTCACCTGTG CCCATCTGCA GAGTTTCGAT GCTGCCCTTT ATCTACAGAT GAATGAGAAG 
AAGCCGACAT GGACGTGTCC TGTGTGTGAC AAGAAGGCTC CCTATGAGTC GCTGATTATT 
GATGGTTTAT TCATGGAAAT TCTTAATTCC TGTTCGGATT GTGATGAGAT CCAGTTCATG 
GAAGATGGAT CCTGGTGTCC GATGAAACCC AAGAAGGAGG CATCAGAGGT TTGCCCCCCG 
CCAGGGTATG GGCTGGATGG TCTCCAGTAC AGCGCAGTCC AGGAGGGAAT TCAGCCAGAG 
AGTAAGAAGA GGGTCGAAGT CATTGACTTG ACCATCGAAA GCTCATCAGA TGAGGAGGAT 
TTGCCCCCCA CCAAGAAGCA CTGCCCTGTC ACCTCAGCGG CCATTCCAGC CCTTCCTGGA 
AGCAAAGGAG CCCTGACCTC TGGTCACCAG CCATCCTCGG TGCTGCGGAG CCCTGCAATG 
GGCACACTGG GCAGTGACTT CCTGTCTAGT CTCCCGCTAC ATGAGTACCC ACCTGCCTTC 
CCACTGGGGG CTGACATCCA AGGTTTAGAT TTATTTTCTT TCCTTCAGAC TGAGAGTCAG 
CACTACGGCC CTTCAGTTAT CACTTCGCTA GATGAACAGG ACACCCTTGG CCACTTCTTC 
CAGTACCGGG GAACCCCTTC CCACTTCCTG GGCCCACTGG CCCCCACACT GGGGAGCTCT 
CACCGCAGCT CCACTCCAGC GCCCCCTCCT GGTCGTGTCA GCAGCATTGT GGCTCCTGGG 
AGCTCCTTGA GGGAAGGGCA TGGAGGACCC CTGCCTTCAG GTCCCTCTTT GACTGGCTGT 
CGGTCAGACG TCATTTCCTT GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001159421.1
CDS	97..1983
Translation	MAELGELKHMVMSFRVSELQVLLGFAGRNKSGRKHELLAKALHLLKSSCAPSVQMKIKELYRRRFPRKTLGPSDLSLLSLPPGTSPVGSPGPLAPIPPTLLTPGTLLGPKREVDMHPPLPQPVHPDVTMKPLPFYEVYGELIRPTTLASTSSQRFEEAHFTFALTPQQLQQILTSREVLPGAKCDYTIQVQLRFCLCETSCPQEDYFPPNLFVKVNGKLCPLPGYLPPTKNGAEPKRPSRPINITPLARLSATVPNTIVVNWSSEFGRNYSLSVYLVRQLTAGTLLQKLRAKGIRNPDHSRALIKEKLTADPDSEVATTSLRVSLMCPLGKMRLTVPCRALTCAHLQSFDAALYLQMNEKKPTWTCPVCDKKAPYESLIIDGLFMEILNSCSDCDEIQFMEDGSWCPMKPKKEASEVCPPPGYGLDGLQYSAVQEGIQPESKKRVEVIDLTIESSSDEEDLPPTKKHCPVTSAAIPALPGSKGALTSGHQPSSVLRSPAMGTLGSDFLSSLPLHEYPPAFPLGADIQGLDLFSFLQTESQHYGPSVITSLDEQDTLGHFFQYRGTPSHFLGPLAPTLGSSHRSSTPAPPPGRVSSIVAPGSSLREGHGGPLPSGPSLTGCRSDVISLD

Target ORF information:

RefSeq Version	NM_001165949.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein inhibitor of activated STAT 3 (Pias3), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001165949.1

ORF Insert Sequence:

ATGGCGGAGC TGGGCGAGTT AAAGCACATG GTGATGAGTT TCCGAGTGTC TGAGCTCCAG 
GTGCTCCTCG GCTTCGCTGG CAGGAACAAG AGTGGGCGGA AACACGAGCT GCTGGCCAAG 
GCCCTGCACC TCCTCAAGTC TAGCTGCGCC CCCAGCGTCC AGATGAAGAT CAAAGAACTT 
TATCGCAGGC GCTTTCCCCG GAAGACCCTG GGGCCTTCTG ATCTCTCCTT GCTTTCTTTG 
CCCCCTGGCA CCTCTCCTGT AGGCTCCCCC GGCCCCCTTG CTCCCATCCC TCCCACCCTT 
CTGACCCCTG GCACCTTGCT AGGCCCTAAG CGTGAGGTGG ACATGCATCC TCCTCTGCCG 
CAGCCTGTGC ACCCCGATGT CACCATGAAG CCACTGCCCT TCTATGAAGT CTATGGGGAG 
CTCATCCGAC CCACCACCCT TGCGTCCACC TCCAGCCAGA GGTTCGAGGA AGCCCACTTC 
ACCTTCGCGC TCACTCCCCA GCAGCTGCAG CAGATTCTCA CGTCCAGGGA GGTTCTGCCA 
GGAGCCAAGT GTGATTACAC CATACAAGTG CAGCTCAGAT TCTGTCTCTG TGAGACCAGC 
TGCCCTCAGG AGGACTATTT CCCCCCTAAC CTCTTTGTTA AGGTTAATGG GAAACTCTGC 
CCCCTGCCGG GTTACCTCCC TCCAACCAAG AATGGAGCTG AGCCCAAGAG GCCCAGCCGT 
CCGATCAACA TCACACCCTT GGCTCGACTC TCAGCCACTG TCCCCAACAC CATCGTAGTT 
AATTGGTCAT CTGAGTTTGG ACGGAATTAC TCCTTGTCCG TGTACCTGGT GAGGCAATTG 
ACTGCAGGGA CCCTTCTACA AAAACTCAGA GCCAAGGGGA TCCGGAATCC AGACCATTCC 
CGGGCACTGA TCAAGGAGAA ACTGACTGCT GACCCCGACA GTGAAGTGGC TACTACAAGT 
CTCCGGGTGT CACTCATGTG CCCGCTAGGG AAGATGCGCC TGACTGTCCC GTGTCGTGCC 
CTCACCTGTG CCCATCTGCA GAGTTTCGAT GCTGCCCTTT ATCTACAGAT GAATGAGAAG 
AAGCCGACAT GGACGTGTCC TGTGTGTGAC AAGAAGGCTC CCTATGAGTC GCTGATTATT 
GATGGTTTAT TCATGGAAAT TCTTAATTCC TGTTCGGATT GTGATGAGAT CCAGTTCATG 
GAAGATGGAT CCTGGTGTCC GATGAAACCC AAGAAGGAGG CATCAGAGGT TTGCCCCCCG 
CCAGGGTATG GGCTGGATGG TCTCCAGTAC AGCGCAGTCC AGGAGGGAAT TCAGCCAGAG 
AGTAAGAAGA GGGTCGAAGT CATTGACTTG ACCATCGAAA GCTCATCAGA TGAGGAGGAT 
TTGCCCCCCA CCAAGAAGCA CTGCCCTGTC ACCTCAGCGG CCATTCCAGC CCTTCCTGGA 
AGCAAAGGAG CCCTGACCTC TGGTCACCAG CCATCCTCGG TGCTGCGGAG CCCTGCAATG 
GGCACACTGG GCAGTGACTT CCTGTCTAGT CTCCCGCTAC ATGAGTACCC ACCTGCCTTC 
CCACTGGGGG CTGACATCCA AGGTTTAGAT TTATTTTCTT TCCTTCAGAC TGAGAGTCAG 
CACTACGGCC CTTCAGTTAT CACTTCGCTA GATGAACAGG ACACCCTTGG CCACTTCTTC 
CAGTACCGGG GAACCCCTTC CCACTTCCTG GGCCCACTGG CCCCCACACT GGGGAGCTCT 
CACCGCAGCT CCACTCCAGC GCCCCCTCCT GGTCGTGTCA GCAGCATTGT GGCTCCTGGG 
AGCTCCTTGA GGGAAGGGCA TGGAGGACCC CTGCCTTCAG GTCCCTCTTT GACTGGCTGT 
CGGTCAGACG TCATTTCCTT GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pias3 ( NM_018812.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_061282.2 Mus musculus protein inhibitor of activated STAT 3 (Pias3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu10987
Clone ID Related Accession (Same CDS sequence)	NM_018812.2
Accession Version	NM_018812.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1782bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-16
Organism	Mus musculus(house mouse)
Product	E3 SUMO-protein ligase PIAS3 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY154092.1, BC050988.1, BE989927.1 and BB042656.1. On Sep 30, 2009 this sequence version replaced NM_018812.1. Transcript Variant: This variant (2) differs in the 5' UTR and 5' coding region, and uses an alternate translational start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1. CCDS Note: The coding region has been updated to extend the N-terminus to one that is supported by available conservation data. The use of an alternative upstream start codon would result in a protein that is 9 aa longer. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC050988.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGAGC TGGGCGAGTT AAAGCACATG GTGATGAGTT TCCGAGTGTC TGAGCTCCAG 
GTGCTCCTCG GCTTCGCTGG CAGGAACAAG AGTGGGCGGA AACACGAGCT GCTGGCCAAG 
GCCCTGCACC TCCTCAAGTC TAGCTGCGCC CCCAGCGTCC AGATGAAGAT CAAAGAACTT 
TATCGCAGGC GCTTTCCCCG GAAGACCCTG GGGCCTTCTG ATCTCTCCTT GCTTTCTTTG 
CCCCCTGGCA CCTCTCCTCC TGTGCACCCC GATGTCACCA TGAAGCCACT GCCCTTCTAT 
GAAGTCTATG GGGAGCTCAT CCGACCCACC ACCCTTGCGT CCACCTCCAG CCAGAGGTTC 
GAGGAAGCCC ACTTCACCTT CGCGCTCACT CCCCAGCAGC TGCAGCAGAT TCTCACGTCC 
AGGGAGGTTC TGCCAGGAGC CAAGTGTGAT TACACCATAC AAGTGCAGCT CAGATTCTGT 
CTCTGTGAGA CCAGCTGCCC TCAGGAGGAC TATTTCCCCC CTAACCTCTT TGTTAAGGTT 
AATGGGAAAC TCTGCCCCCT GCCGGGTTAC CTCCCTCCAA CCAAGAATGG AGCTGAGCCC 
AAGAGGCCCA GCCGTCCGAT CAACATCACA CCCTTGGCTC GACTCTCAGC CACTGTCCCC 
AACACCATCG TAGTTAATTG GTCATCTGAG TTTGGACGGA ATTACTCCTT GTCCGTGTAC 
CTGGTGAGGC AATTGACTGC AGGGACCCTT CTACAAAAAC TCAGAGCCAA GGGGATCCGG 
AATCCAGACC ATTCCCGGGC ACTGATCAAG GAGAAACTGA CTGCTGACCC CGACAGTGAA 
GTGGCTACTA CAAGTCTCCG GGTGTCACTC ATGTGCCCGC TAGGGAAGAT GCGCCTGACT 
GTCCCGTGTC GTGCCCTCAC CTGTGCCCAT CTGCAGAGTT TCGATGCTGC CCTTTATCTA 
CAGATGAATG AGAAGAAGCC GACATGGACG TGTCCTGTGT GTGACAAGAA GGCTCCCTAT 
GAGTCGCTGA TTATTGATGG TTTATTCATG GAAATTCTTA ATTCCTGTTC GGATTGTGAT 
GAGATCCAGT TCATGGAAGA TGGATCCTGG TGTCCGATGA AACCCAAGAA GGAGGCATCA 
GAGGTTTGCC CCCCGCCAGG GTATGGGCTG GATGGTCTCC AGTACAGCGC AGTCCAGGAG 
GGAATTCAGC CAGAGAGTAA GAAGAGGGTC GAAGTCATTG ACTTGACCAT CGAAAGCTCA 
TCAGATGAGG AGGATTTGCC CCCCACCAAG AAGCACTGCC CTGTCACCTC AGCGGCCATT 
CCAGCCCTTC CTGGAAGCAA AGGAGCCCTG ACCTCTGGTC ACCAGCCATC CTCGGTGCTG 
CGGAGCCCTG CAATGGGCAC ACTGGGCAGT GACTTCCTGT CTAGTCTCCC GCTACATGAG 
TACCCACCTG CCTTCCCACT GGGGGCTGAC ATCCAAGGTT TAGATTTATT TTCTTTCCTT 
CAGACTGAGA GTCAGCACTA CGGCCCTTCA GTTATCACTT CGCTAGATGA ACAGGACACC 
CTTGGCCACT TCTTCCAGTA CCGGGGAACC CCTTCCCACT TCCTGGGCCC ACTGGCCCCC 
ACACTGGGGA GCTCTCACCG CAGCTCCACT CCAGCGCCCC CTCCTGGTCG TGTCAGCAGC 
ATTGTGGCTC CTGGGAGCTC CTTGAGGGAA GGGCATGGAG GACCCCTGCC TTCAGGTCCC 
TCTTTGACTG GCTGTCGGTC AGACGTCATT TCCTTGGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061282.2
CDS	97..1878
Translation	MAELGELKHMVMSFRVSELQVLLGFAGRNKSGRKHELLAKALHLLKSSCAPSVQMKIKELYRRRFPRKTLGPSDLSLLSLPPGTSPPVHPDVTMKPLPFYEVYGELIRPTTLASTSSQRFEEAHFTFALTPQQLQQILTSREVLPGAKCDYTIQVQLRFCLCETSCPQEDYFPPNLFVKVNGKLCPLPGYLPPTKNGAEPKRPSRPINITPLARLSATVPNTIVVNWSSEFGRNYSLSVYLVRQLTAGTLLQKLRAKGIRNPDHSRALIKEKLTADPDSEVATTSLRVSLMCPLGKMRLTVPCRALTCAHLQSFDAALYLQMNEKKPTWTCPVCDKKAPYESLIIDGLFMEILNSCSDCDEIQFMEDGSWCPMKPKKEASEVCPPPGYGLDGLQYSAVQEGIQPESKKRVEVIDLTIESSSDEEDLPPTKKHCPVTSAAIPALPGSKGALTSGHQPSSVLRSPAMGTLGSDFLSSLPLHEYPPAFPLGADIQGLDLFSFLQTESQHYGPSVITSLDEQDTLGHFFQYRGTPSHFLGPLAPTLGSSHRSSTPAPPPGRVSSIVAPGSSLREGHGGPLPSGPSLTGCRSDVISLD

Target ORF information:

RefSeq Version	NM_018812.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein inhibitor of activated STAT 3 (Pias3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018812.2

ORF Insert Sequence:

ATGGCGGAGC TGGGCGAGTT AAAGCACATG GTGATGAGTT TCCGAGTGTC TGAGCTCCAG 
GTGCTCCTCG GCTTCGCTGG CAGGAACAAG AGTGGGCGGA AACACGAGCT GCTGGCCAAG 
GCCCTGCACC TCCTCAAGTC TAGCTGCGCC CCCAGCGTCC AGATGAAGAT CAAAGAACTT 
TATCGCAGGC GCTTTCCCCG GAAGACCCTG GGGCCTTCTG ATCTCTCCTT GCTTTCTTTG 
CCCCCTGGCA CCTCTCCTCC TGTGCACCCC GATGTCACCA TGAAGCCACT GCCCTTCTAT 
GAAGTCTATG GGGAGCTCAT CCGACCCACC ACCCTTGCGT CCACCTCCAG CCAGAGGTTC 
GAGGAAGCCC ACTTCACCTT CGCGCTCACT CCCCAGCAGC TGCAGCAGAT TCTCACGTCC 
AGGGAGGTTC TGCCAGGAGC CAAGTGTGAT TACACCATAC AAGTGCAGCT CAGATTCTGT 
CTCTGTGAGA CCAGCTGCCC TCAGGAGGAC TATTTCCCCC CTAACCTCTT TGTTAAGGTT 
AATGGGAAAC TCTGCCCCCT GCCGGGTTAC CTCCCTCCAA CCAAGAATGG AGCTGAGCCC 
AAGAGGCCCA GCCGTCCGAT CAACATCACA CCCTTGGCTC GACTCTCAGC CACTGTCCCC 
AACACCATCG TAGTTAATTG GTCATCTGAG TTTGGACGGA ATTACTCCTT GTCCGTGTAC 
CTGGTGAGGC AATTGACTGC AGGGACCCTT CTACAAAAAC TCAGAGCCAA GGGGATCCGG 
AATCCAGACC ATTCCCGGGC ACTGATCAAG GAGAAACTGA CTGCTGACCC CGACAGTGAA 
GTGGCTACTA CAAGTCTCCG GGTGTCACTC ATGTGCCCGC TAGGGAAGAT GCGCCTGACT 
GTCCCGTGTC GTGCCCTCAC CTGTGCCCAT CTGCAGAGTT TCGATGCTGC CCTTTATCTA 
CAGATGAATG AGAAGAAGCC GACATGGACG TGTCCTGTGT GTGACAAGAA GGCTCCCTAT 
GAGTCGCTGA TTATTGATGG TTTATTCATG GAAATTCTTA ATTCCTGTTC GGATTGTGAT 
GAGATCCAGT TCATGGAAGA TGGATCCTGG TGTCCGATGA AACCCAAGAA GGAGGCATCA 
GAGGTTTGCC CCCCGCCAGG GTATGGGCTG GATGGTCTCC AGTACAGCGC AGTCCAGGAG 
GGAATTCAGC CAGAGAGTAA GAAGAGGGTC GAAGTCATTG ACTTGACCAT CGAAAGCTCA 
TCAGATGAGG AGGATTTGCC CCCCACCAAG AAGCACTGCC CTGTCACCTC AGCGGCCATT 
CCAGCCCTTC CTGGAAGCAA AGGAGCCCTG ACCTCTGGTC ACCAGCCATC CTCGGTGCTG 
CGGAGCCCTG CAATGGGCAC ACTGGGCAGT GACTTCCTGT CTAGTCTCCC GCTACATGAG 
TACCCACCTG CCTTCCCACT GGGGGCTGAC ATCCAAGGTT TAGATTTATT TTCTTTCCTT 
CAGACTGAGA GTCAGCACTA CGGCCCTTCA GTTATCACTT CGCTAGATGA ACAGGACACC 
CTTGGCCACT TCTTCCAGTA CCGGGGAACC CCTTCCCACT TCCTGGGCCC ACTGGCCCCC 
ACACTGGGGA GCTCTCACCG CAGCTCCACT CCAGCGCCCC CTCCTGGTCG TGTCAGCAGC 
ATTGTGGCTC CTGGGAGCTC CTTGAGGGAA GGGCATGGAG GACCCCTGCC TTCAGGTCCC 
TCTTTGACTG GCTGTCGGTC AGACGTCATT TCCTTGGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pias3 ( NM_146135.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_666247.1 Mus musculus protein inhibitor of activated STAT 3 (Pias3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu12254
Clone ID Related Accession (Same CDS sequence)	XM_006501393.1 , NM_146135.2
Accession Version	NM_146135.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-15
Organism	Mus musculus(house mouse)
Product	E3 SUMO-protein ligase PIAS3 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY002850.1, AK040619.1, BE989927.1 and BB042656.1. On Sep 30, 2009 this sequence version replaced NM_146135.1. Transcript Variant: This variant (1) encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK077551.1, AK040619.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGATGA GTTTCCGAGT GTCTGAGCTC CAGGTGCTCC TCGGCTTCGC TGGCAGGAAC 
AAGAGTGGGC GGAAACACGA GCTGCTGGCC AAGGCCCTGC ACCTCCTCAA GTCTAGCTGC 
GCCCCCAGCG TCCAGATGAA GATCAAAGAA CTTTATCGCA GGCGCTTTCC CCGGAAGACC 
CTGGGGCCTT CTGATCTCTC CTTGCTTTCT TTGCCCCCTG GCACCTCTCC TGTAGGCTCC 
CCCGGCCCCC TTGCTCCCAT CCCTCCCACC CTTCTGACCC CTGGCACCTT GCTAGGCCCT 
AAGCGTGAGG TGGACATGCA TCCTCCTCTG CCGCAGCCTG TGCACCCCGA TGTCACCATG 
AAGCCACTGC CCTTCTATGA AGTCTATGGG GAGCTCATCC GACCCACCAC CCTTGCGTCC 
ACCTCCAGCC AGAGGTTCGA GGAAGCCCAC TTCACCTTCG CGCTCACTCC CCAGCAGCTG 
CAGCAGATTC TCACGTCCAG GGAGGTTCTG CCAGGAGCCA AGTGTGATTA CACCATACAA 
GTGCAGCTCA GATTCTGTCT CTGTGAGACC AGCTGCCCTC AGGAGGACTA TTTCCCCCCT 
AACCTCTTTG TTAAGGTTAA TGGGAAACTC TGCCCCCTGC CGGGTTACCT CCCTCCAACC 
AAGAATGGAG CTGAGCCCAA GAGGCCCAGC CGTCCGATCA ACATCACACC CTTGGCTCGA 
CTCTCAGCCA CTGTCCCCAA CACCATCGTA GTTAATTGGT CATCTGAGTT TGGACGGAAT 
TACTCCTTGT CCGTGTACCT GGTGAGGCAA TTGACTGCAG GGACCCTTCT ACAAAAACTC 
AGAGCCAAGG GGATCCGGAA TCCAGACCAT TCCCGGGCAC TGATCAAGGA GAAACTGACT 
GCTGACCCCG ACAGTGAAGT GGCTACTACA AGTCTCCGGG TGTCACTCAT GTGCCCGCTA 
GGGAAGATGC GCCTGACTGT CCCGTGTCGT GCCCTCACCT GTGCCCATCT GCAGAGTTTC 
GATGCTGCCC TTTATCTACA GATGAATGAG AAGAAGCCGA CATGGACGTG TCCTGTGTGT 
GACAAGAAGG CTCCCTATGA GTCGCTGATT ATTGATGGTT TATTCATGGA AATTCTTAAT 
TCCTGTTCGG ATTGTGATGA GATCCAGTTC ATGGAAGATG GATCCTGGTG TCCGATGAAA 
CCCAAGAAGG AGGCATCAGA GGTTTGCCCC CCGCCAGGGT ATGGGCTGGA TGGTCTCCAG 
TACAGCGCAG TCCAGGAGGG AATTCAGCCA GAGAGTAAGA AGAGGGTCGA AGTCATTGAC 
TTGACCATCG AAAGCTCATC AGATGAGGAG GATTTGCCCC CCACCAAGAA GCACTGCCCT 
GTCACCTCAG CGGCCATTCC AGCCCTTCCT GGAAGCAAAG GAGCCCTGAC CTCTGGTCAC 
CAGCCATCCT CGGTGCTGCG GAGCCCTGCA ATGGGCACAC TGGGCAGTGA CTTCCTGTCT 
AGTCTCCCGC TACATGAGTA CCCACCTGCC TTCCCACTGG GGGCTGACAT CCAAGGTTTA 
GATTTATTTT CTTTCCTTCA GACTGAGAGT CAGCACTACG GCCCTTCAGT TATCACTTCG 
CTAGATGAAC AGGACACCCT TGGCCACTTC TTCCAGTACC GGGGAACCCC TTCCCACTTC 
CTGGGCCCAC TGGCCCCCAC ACTGGGGAGC TCTCACCGCA GCTCCACTCC AGCGCCCCCT 
CCTGGTCGTG TCAGCAGCAT TGTGGCTCCT GGGAGCTCCT TGAGGGAAGG GCATGGAGGA 
CCCCTGCCTT CAGGTCCCTC TTTGACTGGC TGTCGGTCAG ACGTCATTTC CTTGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_666247.1
CDS	161..2020
Translation	MVMSFRVSELQVLLGFAGRNKSGRKHELLAKALHLLKSSCAPSVQMKIKELYRRRFPRKTLGPSDLSLLSLPPGTSPVGSPGPLAPIPPTLLTPGTLLGPKREVDMHPPLPQPVHPDVTMKPLPFYEVYGELIRPTTLASTSSQRFEEAHFTFALTPQQLQQILTSREVLPGAKCDYTIQVQLRFCLCETSCPQEDYFPPNLFVKVNGKLCPLPGYLPPTKNGAEPKRPSRPINITPLARLSATVPNTIVVNWSSEFGRNYSLSVYLVRQLTAGTLLQKLRAKGIRNPDHSRALIKEKLTADPDSEVATTSLRVSLMCPLGKMRLTVPCRALTCAHLQSFDAALYLQMNEKKPTWTCPVCDKKAPYESLIIDGLFMEILNSCSDCDEIQFMEDGSWCPMKPKKEASEVCPPPGYGLDGLQYSAVQEGIQPESKKRVEVIDLTIESSSDEEDLPPTKKHCPVTSAAIPALPGSKGALTSGHQPSSVLRSPAMGTLGSDFLSSLPLHEYPPAFPLGADIQGLDLFSFLQTESQHYGPSVITSLDEQDTLGHFFQYRGTPSHFLGPLAPTLGSSHRSSTPAPPPGRVSSIVAPGSSLREGHGGPLPSGPSLTGCRSDVISLD

Target ORF information:

RefSeq Version	NM_146135.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein inhibitor of activated STAT 3 (Pias3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_146135.2

ORF Insert Sequence:

ATGGTGATGA GTTTCCGAGT GTCTGAGCTC CAGGTGCTCC TCGGCTTCGC TGGCAGGAAC 
AAGAGTGGGC GGAAACACGA GCTGCTGGCC AAGGCCCTGC ACCTCCTCAA GTCTAGCTGC 
GCCCCCAGCG TCCAGATGAA GATCAAAGAA CTTTATCGCA GGCGCTTTCC CCGGAAGACC 
CTGGGGCCTT CTGATCTCTC CTTGCTTTCT TTGCCCCCTG GCACCTCTCC TGTAGGCTCC 
CCCGGCCCCC TTGCTCCCAT CCCTCCCACC CTTCTGACCC CTGGCACCTT GCTAGGCCCT 
AAGCGTGAGG TGGACATGCA TCCTCCTCTG CCGCAGCCTG TGCACCCCGA TGTCACCATG 
AAGCCACTGC CCTTCTATGA AGTCTATGGG GAGCTCATCC GACCCACCAC CCTTGCGTCC 
ACCTCCAGCC AGAGGTTCGA GGAAGCCCAC TTCACCTTCG CGCTCACTCC CCAGCAGCTG 
CAGCAGATTC TCACGTCCAG GGAGGTTCTG CCAGGAGCCA AGTGTGATTA CACCATACAA 
GTGCAGCTCA GATTCTGTCT CTGTGAGACC AGCTGCCCTC AGGAGGACTA TTTCCCCCCT 
AACCTCTTTG TTAAGGTTAA TGGGAAACTC TGCCCCCTGC CGGGTTACCT CCCTCCAACC 
AAGAATGGAG CTGAGCCCAA GAGGCCCAGC CGTCCGATCA ACATCACACC CTTGGCTCGA 
CTCTCAGCCA CTGTCCCCAA CACCATCGTA GTTAATTGGT CATCTGAGTT TGGACGGAAT 
TACTCCTTGT CCGTGTACCT GGTGAGGCAA TTGACTGCAG GGACCCTTCT ACAAAAACTC 
AGAGCCAAGG GGATCCGGAA TCCAGACCAT TCCCGGGCAC TGATCAAGGA GAAACTGACT 
GCTGACCCCG ACAGTGAAGT GGCTACTACA AGTCTCCGGG TGTCACTCAT GTGCCCGCTA 
GGGAAGATGC GCCTGACTGT CCCGTGTCGT GCCCTCACCT GTGCCCATCT GCAGAGTTTC 
GATGCTGCCC TTTATCTACA GATGAATGAG AAGAAGCCGA CATGGACGTG TCCTGTGTGT 
GACAAGAAGG CTCCCTATGA GTCGCTGATT ATTGATGGTT TATTCATGGA AATTCTTAAT 
TCCTGTTCGG ATTGTGATGA GATCCAGTTC ATGGAAGATG GATCCTGGTG TCCGATGAAA 
CCCAAGAAGG AGGCATCAGA GGTTTGCCCC CCGCCAGGGT ATGGGCTGGA TGGTCTCCAG 
TACAGCGCAG TCCAGGAGGG AATTCAGCCA GAGAGTAAGA AGAGGGTCGA AGTCATTGAC 
TTGACCATCG AAAGCTCATC AGATGAGGAG GATTTGCCCC CCACCAAGAA GCACTGCCCT 
GTCACCTCAG CGGCCATTCC AGCCCTTCCT GGAAGCAAAG GAGCCCTGAC CTCTGGTCAC 
CAGCCATCCT CGGTGCTGCG GAGCCCTGCA ATGGGCACAC TGGGCAGTGA CTTCCTGTCT 
AGTCTCCCGC TACATGAGTA CCCACCTGCC TTCCCACTGG GGGCTGACAT CCAAGGTTTA 
GATTTATTTT CTTTCCTTCA GACTGAGAGT CAGCACTACG GCCCTTCAGT TATCACTTCG 
CTAGATGAAC AGGACACCCT TGGCCACTTC TTCCAGTACC GGGGAACCCC TTCCCACTTC 
CTGGGCCCAC TGGCCCCCAC ACTGGGGAGC TCTCACCGCA GCTCCACTCC AGCGCCCCCT 
CCTGGTCGTG TCAGCAGCAT TGTGGCTCCT GGGAGCTCCT TGAGGGAAGG GCATGGAGGA 
CCCCTGCCTT CAGGTCCCTC TTTGACTGGC TGTCGGTCAG ACGTCATTTC CTTGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pias3 ( XM_006501393.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501456.1 Mus musculus protein inhibitor of activated STAT 3 (Pias3), transcript variant X1, mRNA.

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CloneID	OMu12254
Clone ID Related Accession (Same CDS sequence)	XM_006501393.1 , NM_146135.2
Accession Version	XM_006501393.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 SUMO-protein ligase PIAS3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGATGA GTTTCCGAGT GTCTGAGCTC CAGGTGCTCC TCGGCTTCGC TGGCAGGAAC 
AAGAGTGGGC GGAAACACGA GCTGCTGGCC AAGGCCCTGC ACCTCCTCAA GTCTAGCTGC 
GCCCCCAGCG TCCAGATGAA GATCAAAGAA CTTTATCGCA GGCGCTTTCC CCGGAAGACC 
CTGGGGCCTT CTGATCTCTC CTTGCTTTCT TTGCCCCCTG GCACCTCTCC TGTAGGCTCC 
CCCGGCCCCC TTGCTCCCAT CCCTCCCACC CTTCTGACCC CTGGCACCTT GCTAGGCCCT 
AAGCGTGAGG TGGACATGCA TCCTCCTCTG CCGCAGCCTG TGCACCCCGA TGTCACCATG 
AAGCCACTGC CCTTCTATGA AGTCTATGGG GAGCTCATCC GACCCACCAC CCTTGCGTCC 
ACCTCCAGCC AGAGGTTCGA GGAAGCCCAC TTCACCTTCG CGCTCACTCC CCAGCAGCTG 
CAGCAGATTC TCACGTCCAG GGAGGTTCTG CCAGGAGCCA AGTGTGATTA CACCATACAA 
GTGCAGCTCA GATTCTGTCT CTGTGAGACC AGCTGCCCTC AGGAGGACTA TTTCCCCCCT 
AACCTCTTTG TTAAGGTTAA TGGGAAACTC TGCCCCCTGC CGGGTTACCT CCCTCCAACC 
AAGAATGGAG CTGAGCCCAA GAGGCCCAGC CGTCCGATCA ACATCACACC CTTGGCTCGA 
CTCTCAGCCA CTGTCCCCAA CACCATCGTA GTTAATTGGT CATCTGAGTT TGGACGGAAT 
TACTCCTTGT CCGTGTACCT GGTGAGGCAA TTGACTGCAG GGACCCTTCT ACAAAAACTC 
AGAGCCAAGG GGATCCGGAA TCCAGACCAT TCCCGGGCAC TGATCAAGGA GAAACTGACT 
GCTGACCCCG ACAGTGAAGT GGCTACTACA AGTCTCCGGG TGTCACTCAT GTGCCCGCTA 
GGGAAGATGC GCCTGACTGT CCCGTGTCGT GCCCTCACCT GTGCCCATCT GCAGAGTTTC 
GATGCTGCCC TTTATCTACA GATGAATGAG AAGAAGCCGA CATGGACGTG TCCTGTGTGT 
GACAAGAAGG CTCCCTATGA GTCGCTGATT ATTGATGGTT TATTCATGGA AATTCTTAAT 
TCCTGTTCGG ATTGTGATGA GATCCAGTTC ATGGAAGATG GATCCTGGTG TCCGATGAAA 
CCCAAGAAGG AGGCATCAGA GGTTTGCCCC CCGCCAGGGT ATGGGCTGGA TGGTCTCCAG 
TACAGCGCAG TCCAGGAGGG AATTCAGCCA GAGAGTAAGA AGAGGGTCGA AGTCATTGAC 
TTGACCATCG AAAGCTCATC AGATGAGGAG GATTTGCCCC CCACCAAGAA GCACTGCCCT 
GTCACCTCAG CGGCCATTCC AGCCCTTCCT GGAAGCAAAG GAGCCCTGAC CTCTGGTCAC 
CAGCCATCCT CGGTGCTGCG GAGCCCTGCA ATGGGCACAC TGGGCAGTGA CTTCCTGTCT 
AGTCTCCCGC TACATGAGTA CCCACCTGCC TTCCCACTGG GGGCTGACAT CCAAGGTTTA 
GATTTATTTT CTTTCCTTCA GACTGAGAGT CAGCACTACG GCCCTTCAGT TATCACTTCG 
CTAGATGAAC AGGACACCCT TGGCCACTTC TTCCAGTACC GGGGAACCCC TTCCCACTTC 
CTGGGCCCAC TGGCCCCCAC ACTGGGGAGC TCTCACCGCA GCTCCACTCC AGCGCCCCCT 
CCTGGTCGTG TCAGCAGCAT TGTGGCTCCT GGGAGCTCCT TGAGGGAAGG GCATGGAGGA 
CCCCTGCCTT CAGGTCCCTC TTTGACTGGC TGTCGGTCAG ACGTCATTTC CTTGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501456.1
CDS	785..2644
Translation	MVMSFRVSELQVLLGFAGRNKSGRKHELLAKALHLLKSSCAPSVQMKIKELYRRRFPRKTLGPSDLSLLSLPPGTSPVGSPGPLAPIPPTLLTPGTLLGPKREVDMHPPLPQPVHPDVTMKPLPFYEVYGELIRPTTLASTSSQRFEEAHFTFALTPQQLQQILTSREVLPGAKCDYTIQVQLRFCLCETSCPQEDYFPPNLFVKVNGKLCPLPGYLPPTKNGAEPKRPSRPINITPLARLSATVPNTIVVNWSSEFGRNYSLSVYLVRQLTAGTLLQKLRAKGIRNPDHSRALIKEKLTADPDSEVATTSLRVSLMCPLGKMRLTVPCRALTCAHLQSFDAALYLQMNEKKPTWTCPVCDKKAPYESLIIDGLFMEILNSCSDCDEIQFMEDGSWCPMKPKKEASEVCPPPGYGLDGLQYSAVQEGIQPESKKRVEVIDLTIESSSDEEDLPPTKKHCPVTSAAIPALPGSKGALTSGHQPSSVLRSPAMGTLGSDFLSSLPLHEYPPAFPLGADIQGLDLFSFLQTESQHYGPSVITSLDEQDTLGHFFQYRGTPSHFLGPLAPTLGSSHRSSTPAPPPGRVSSIVAPGSSLREGHGGPLPSGPSLTGCRSDVISLD

Target ORF information:

RefSeq Version	XM_006501393.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein inhibitor of activated STAT 3 (Pias3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501393.1

ORF Insert Sequence:

ATGGTGATGA GTTTCCGAGT GTCTGAGCTC CAGGTGCTCC TCGGCTTCGC TGGCAGGAAC 
AAGAGTGGGC GGAAACACGA GCTGCTGGCC AAGGCCCTGC ACCTCCTCAA GTCTAGCTGC 
GCCCCCAGCG TCCAGATGAA GATCAAAGAA CTTTATCGCA GGCGCTTTCC CCGGAAGACC 
CTGGGGCCTT CTGATCTCTC CTTGCTTTCT TTGCCCCCTG GCACCTCTCC TGTAGGCTCC 
CCCGGCCCCC TTGCTCCCAT CCCTCCCACC CTTCTGACCC CTGGCACCTT GCTAGGCCCT 
AAGCGTGAGG TGGACATGCA TCCTCCTCTG CCGCAGCCTG TGCACCCCGA TGTCACCATG 
AAGCCACTGC CCTTCTATGA AGTCTATGGG GAGCTCATCC GACCCACCAC CCTTGCGTCC 
ACCTCCAGCC AGAGGTTCGA GGAAGCCCAC TTCACCTTCG CGCTCACTCC CCAGCAGCTG 
CAGCAGATTC TCACGTCCAG GGAGGTTCTG CCAGGAGCCA AGTGTGATTA CACCATACAA 
GTGCAGCTCA GATTCTGTCT CTGTGAGACC AGCTGCCCTC AGGAGGACTA TTTCCCCCCT 
AACCTCTTTG TTAAGGTTAA TGGGAAACTC TGCCCCCTGC CGGGTTACCT CCCTCCAACC 
AAGAATGGAG CTGAGCCCAA GAGGCCCAGC CGTCCGATCA ACATCACACC CTTGGCTCGA 
CTCTCAGCCA CTGTCCCCAA CACCATCGTA GTTAATTGGT CATCTGAGTT TGGACGGAAT 
TACTCCTTGT CCGTGTACCT GGTGAGGCAA TTGACTGCAG GGACCCTTCT ACAAAAACTC 
AGAGCCAAGG GGATCCGGAA TCCAGACCAT TCCCGGGCAC TGATCAAGGA GAAACTGACT 
GCTGACCCCG ACAGTGAAGT GGCTACTACA AGTCTCCGGG TGTCACTCAT GTGCCCGCTA 
GGGAAGATGC GCCTGACTGT CCCGTGTCGT GCCCTCACCT GTGCCCATCT GCAGAGTTTC 
GATGCTGCCC TTTATCTACA GATGAATGAG AAGAAGCCGA CATGGACGTG TCCTGTGTGT 
GACAAGAAGG CTCCCTATGA GTCGCTGATT ATTGATGGTT TATTCATGGA AATTCTTAAT 
TCCTGTTCGG ATTGTGATGA GATCCAGTTC ATGGAAGATG GATCCTGGTG TCCGATGAAA 
CCCAAGAAGG AGGCATCAGA GGTTTGCCCC CCGCCAGGGT ATGGGCTGGA TGGTCTCCAG 
TACAGCGCAG TCCAGGAGGG AATTCAGCCA GAGAGTAAGA AGAGGGTCGA AGTCATTGAC 
TTGACCATCG AAAGCTCATC AGATGAGGAG GATTTGCCCC CCACCAAGAA GCACTGCCCT 
GTCACCTCAG CGGCCATTCC AGCCCTTCCT GGAAGCAAAG GAGCCCTGAC CTCTGGTCAC 
CAGCCATCCT CGGTGCTGCG GAGCCCTGCA ATGGGCACAC TGGGCAGTGA CTTCCTGTCT 
AGTCTCCCGC TACATGAGTA CCCACCTGCC TTCCCACTGG GGGCTGACAT CCAAGGTTTA 
GATTTATTTT CTTTCCTTCA GACTGAGAGT CAGCACTACG GCCCTTCAGT TATCACTTCG 
CTAGATGAAC AGGACACCCT TGGCCACTTC TTCCAGTACC GGGGAACCCC TTCCCACTTC 
CTGGGCCCAC TGGCCCCCAC ACTGGGGAGC TCTCACCGCA GCTCCACTCC AGCGCCCCCT 
CCTGGTCGTG TCAGCAGCAT TGTGGCTCCT GGGAGCTCCT TGAGGGAAGG GCATGGAGGA 
CCCCTGCCTT CAGGTCCCTC TTTGACTGGC TGTCGGTCAG ACGTCATTTC CTTGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pias3 ( XM_030252586.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108446.1 Mus musculus protein inhibitor of activated STAT 3 (Pias3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu88219
Clone ID Related Accession (Same CDS sequence)	XM_030252586.1
Accession Version	XM_030252586.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1755bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 SUMO-protein ligase PIAS3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGATGA GTTTCCGAGT GTCTGAGCTC CAGGTGCTCC TCGGCTTCGC TGGCAGGAAC 
AAGAGTGGGC GGAAACACGA GCTGCTGGCC AAGGCCCTGC ACCTCCTCAA GTCTAGCTGC 
GCCCCCAGCG TCCAGATGAA GATCAAAGAA CTTTATCGCA GGCGCTTTCC CCGGAAGACC 
CTGGGGCCTT CTGATCTCTC CTTGCTTTCT TTGCCCCCTG GCACCTCTCC TCCTGTGCAC 
CCCGATGTCA CCATGAAGCC ACTGCCCTTC TATGAAGTCT ATGGGGAGCT CATCCGACCC 
ACCACCCTTG CGTCCACCTC CAGCCAGAGG TTCGAGGAAG CCCACTTCAC CTTCGCGCTC 
ACTCCCCAGC AGCTGCAGCA GATTCTCACG TCCAGGGAGG TTCTGCCAGG AGCCAAGTGT 
GATTACACCA TACAAGTGCA GCTCAGATTC TGTCTCTGTG AGACCAGCTG CCCTCAGGAG 
GACTATTTCC CCCCTAACCT CTTTGTTAAG GTTAATGGGA AACTCTGCCC CCTGCCGGGT 
TACCTCCCTC CAACCAAGAA TGGAGCTGAG CCCAAGAGGC CCAGCCGTCC GATCAACATC 
ACACCCTTGG CTCGACTCTC AGCCACTGTC CCCAACACCA TCGTAGTTAA TTGGTCATCT 
GAGTTTGGAC GGAATTACTC CTTGTCCGTG TACCTGGTGA GGCAATTGAC TGCAGGGACC 
CTTCTACAAA AACTCAGAGC CAAGGGGATC CGGAATCCAG ACCATTCCCG GGCACTGATC 
AAGGAGAAAC TGACTGCTGA CCCCGACAGT GAAGTGGCTA CTACAAGTCT CCGGGTGTCA 
CTCATGTGCC CGCTAGGGAA GATGCGCCTG ACTGTCCCGT GTCGTGCCCT CACCTGTGCC 
CATCTGCAGA GTTTCGATGC TGCCCTTTAT CTACAGATGA ATGAGAAGAA GCCGACATGG 
ACGTGTCCTG TGTGTGACAA GAAGGCTCCC TATGAGTCGC TGATTATTGA TGGTTTATTC 
ATGGAAATTC TTAATTCCTG TTCGGATTGT GATGAGATCC AGTTCATGGA AGATGGATCC 
TGGTGTCCGA TGAAACCCAA GAAGGAGGCA TCAGAGGTTT GCCCCCCGCC AGGGTATGGG 
CTGGATGGTC TCCAGTACAG CGCAGTCCAG GAGGGAATTC AGCCAGAGAG TAAGAAGAGG 
GTCGAAGTCA TTGACTTGAC CATCGAAAGC TCATCAGATG AGGAGGATTT GCCCCCCACC 
AAGAAGCACT GCCCTGTCAC CTCAGCGGCC ATTCCAGCCC TTCCTGGAAG CAAAGGAGCC 
CTGACCTCTG GTCACCAGCC ATCCTCGGTG CTGCGGAGCC CTGCAATGGG CACACTGGGC 
AGTGACTTCC TGTCTAGTCT CCCGCTACAT GAGTACCCAC CTGCCTTCCC ACTGGGGGCT 
GACATCCAAG GTTTAGATTT ATTTTCTTTC CTTCAGACTG AGAGTCAGCA CTACGGCCCT 
TCAGTTATCA CTTCGCTAGA TGAACAGGAC ACCCTTGGCC ACTTCTTCCA GTACCGGGGA 
ACCCCTTCCC ACTTCCTGGG CCCACTGGCC CCCACACTGG GGAGCTCTCA CCGCAGCTCC 
ACTCCAGCGC CCCCTCCTGG TCGTGTCAGC AGCATTGTGG CTCCTGGGAG CTCCTTGAGG 
GAAGGGCATG GAGGACCCCT GCCTTCAGGT CCCTCTTTGA CTGGCTGTCG GTCAGACGTC 
ATTTCCTTGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108446.1
CDS	175..1929
Translation	MVMSFRVSELQVLLGFAGRNKSGRKHELLAKALHLLKSSCAPSVQMKIKELYRRRFPRKTLGPSDLSLLSLPPGTSPPVHPDVTMKPLPFYEVYGELIRPTTLASTSSQRFEEAHFTFALTPQQLQQILTSREVLPGAKCDYTIQVQLRFCLCETSCPQEDYFPPNLFVKVNGKLCPLPGYLPPTKNGAEPKRPSRPINITPLARLSATVPNTIVVNWSSEFGRNYSLSVYLVRQLTAGTLLQKLRAKGIRNPDHSRALIKEKLTADPDSEVATTSLRVSLMCPLGKMRLTVPCRALTCAHLQSFDAALYLQMNEKKPTWTCPVCDKKAPYESLIIDGLFMEILNSCSDCDEIQFMEDGSWCPMKPKKEASEVCPPPGYGLDGLQYSAVQEGIQPESKKRVEVIDLTIESSSDEEDLPPTKKHCPVTSAAIPALPGSKGALTSGHQPSSVLRSPAMGTLGSDFLSSLPLHEYPPAFPLGADIQGLDLFSFLQTESQHYGPSVITSLDEQDTLGHFFQYRGTPSHFLGPLAPTLGSSHRSSTPAPPPGRVSSIVAPGSSLREGHGGPLPSGPSLTGCRSDVISLD

Target ORF information:

RefSeq Version	XM_030252586.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein inhibitor of activated STAT 3 (Pias3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252586.1

ORF Insert Sequence:

ATGGTGATGA GTTTCCGAGT GTCTGAGCTC CAGGTGCTCC TCGGCTTCGC TGGCAGGAAC 
AAGAGTGGGC GGAAACACGA GCTGCTGGCC AAGGCCCTGC ACCTCCTCAA GTCTAGCTGC 
GCCCCCAGCG TCCAGATGAA GATCAAAGAA CTTTATCGCA GGCGCTTTCC CCGGAAGACC 
CTGGGGCCTT CTGATCTCTC CTTGCTTTCT TTGCCCCCTG GCACCTCTCC TCCTGTGCAC 
CCCGATGTCA CCATGAAGCC ACTGCCCTTC TATGAAGTCT ATGGGGAGCT CATCCGACCC 
ACCACCCTTG CGTCCACCTC CAGCCAGAGG TTCGAGGAAG CCCACTTCAC CTTCGCGCTC 
ACTCCCCAGC AGCTGCAGCA GATTCTCACG TCCAGGGAGG TTCTGCCAGG AGCCAAGTGT 
GATTACACCA TACAAGTGCA GCTCAGATTC TGTCTCTGTG AGACCAGCTG CCCTCAGGAG 
GACTATTTCC CCCCTAACCT CTTTGTTAAG GTTAATGGGA AACTCTGCCC CCTGCCGGGT 
TACCTCCCTC CAACCAAGAA TGGAGCTGAG CCCAAGAGGC CCAGCCGTCC GATCAACATC 
ACACCCTTGG CTCGACTCTC AGCCACTGTC CCCAACACCA TCGTAGTTAA TTGGTCATCT 
GAGTTTGGAC GGAATTACTC CTTGTCCGTG TACCTGGTGA GGCAATTGAC TGCAGGGACC 
CTTCTACAAA AACTCAGAGC CAAGGGGATC CGGAATCCAG ACCATTCCCG GGCACTGATC 
AAGGAGAAAC TGACTGCTGA CCCCGACAGT GAAGTGGCTA CTACAAGTCT CCGGGTGTCA 
CTCATGTGCC CGCTAGGGAA GATGCGCCTG ACTGTCCCGT GTCGTGCCCT CACCTGTGCC 
CATCTGCAGA GTTTCGATGC TGCCCTTTAT CTACAGATGA ATGAGAAGAA GCCGACATGG 
ACGTGTCCTG TGTGTGACAA GAAGGCTCCC TATGAGTCGC TGATTATTGA TGGTTTATTC 
ATGGAAATTC TTAATTCCTG TTCGGATTGT GATGAGATCC AGTTCATGGA AGATGGATCC 
TGGTGTCCGA TGAAACCCAA GAAGGAGGCA TCAGAGGTTT GCCCCCCGCC AGGGTATGGG 
CTGGATGGTC TCCAGTACAG CGCAGTCCAG GAGGGAATTC AGCCAGAGAG TAAGAAGAGG 
GTCGAAGTCA TTGACTTGAC CATCGAAAGC TCATCAGATG AGGAGGATTT GCCCCCCACC 
AAGAAGCACT GCCCTGTCAC CTCAGCGGCC ATTCCAGCCC TTCCTGGAAG CAAAGGAGCC 
CTGACCTCTG GTCACCAGCC ATCCTCGGTG CTGCGGAGCC CTGCAATGGG CACACTGGGC 
AGTGACTTCC TGTCTAGTCT CCCGCTACAT GAGTACCCAC CTGCCTTCCC ACTGGGGGCT 
GACATCCAAG GTTTAGATTT ATTTTCTTTC CTTCAGACTG AGAGTCAGCA CTACGGCCCT 
TCAGTTATCA CTTCGCTAGA TGAACAGGAC ACCCTTGGCC ACTTCTTCCA GTACCGGGGA 
ACCCCTTCCC ACTTCCTGGG CCCACTGGCC CCCACACTGG GGAGCTCTCA CCGCAGCTCC 
ACTCCAGCGC CCCCTCCTGG TCGTGTCAGC AGCATTGTGG CTCCTGGGAG CTCCTTGAGG 
GAAGGGCATG GAGGACCCCT GCCTTCAGGT CCCTCTTTGA CTGGCTGTCG GTCAGACGTC 
ATTTCCTTGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Foxp1 regulation of neonatal vocalizations via cortical development.
Genes & development31(20)2039-2055(2017 Oct)
Usui N,Araujo DJ,Kulkarni A,Co M,Ellegood J,Harper M,Toriumi K,Lerch JP,Konopka G

Pias3 is necessary for dorso-ventral patterning and visual response of retinal cones but is not required for rod photoreceptor differentiation.
Biology open6(6)881-890(2017 Jun)
Campla CK,Breit H,Dong L,Gumerson JD,Roger JE,Swaroop A

Protein Inhibitor of Activated STAT3 Suppresses Oxidized LDL-induced Cell Responses during Atherosclerosis in Apolipoprotein E-deficient Mice.
Scientific reports636790(2016 Nov)
Wang R,Zhang Y,Xu L,Lin Y,Yang X,Bai L,Chen Y,Zhao S,Fan J,Cheng X,Liu E

Transcriptional activity of erythroid Kruppel-like factor (EKLF/KLF1) modulated by PIAS3 (protein inhibitor of activated STAT3).
The Journal of biological chemistry290(15)9929-40(2015 Apr)
Siatecka M,Soni S,Planutis A,Bieker JJ

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Pias3 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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