Gpr149 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gpr149
Entrez Gene ID	229357
Full Name	G protein-coupled receptor 149
Synonyms	9630018L10Rik,Ieda,PGR10,R35
General protein information	Preferred Names G protein-coupled receptor 149 Names probable G-protein coupled receptor 149 G protein-coupled receptor PGR10
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3\|3 E1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006501322.3	XP_006501385.1	probable G-protein coupled receptor 149 isoform X1
NM_177346.5	NP_796320.2	probable G-protein coupled receptor 149 isoform 1
NM_177346.4	NP_796320.2	probable G-protein coupled receptor 149
XM_011240076.1	XP_011238378.1	probable G-protein coupled receptor 149 isoform X2
XM_011240076.2	XP_011238378.1	probable G-protein coupled receptor 149 isoform X1
NM_001356525.1	NP_001343454.1	probable G-protein coupled receptor 149 isoform 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GPR149	XP_526355.2
Mus musculus (house mouse)	Gpr149	NP_796320.2
Gallus gallus (chicken)	GPR149	NP_989993.1
Xenopus tropicalis (tropical clawed frog)	gpr149	XP_002933305.2
Bos taurus (cattle)	GPR149	XP_003585740.1
Rattus norvegicus (Norway rat)	Gpr149	NP_620246.1
Homo sapiens (human)	GPR149	NP_001033794.1
Canis lupus familiaris (dog)	GPR149	XP_534312.2

Gene Ontology
Bibliography

Related articles in PubMed

Deletion of the novel oocyte-enriched gene, Gpr149, leads to increased fertility in mice.
Edson MA, Lin YN, Matzuk MM
Endocrinology151(1)358-68(2010 Jan)

The neonatal ventromedial hypothalamus transcriptome reveals novel markers with spatially distinct patterning.
Kurrasch DM, Cheung CC, Lee FY, Tran PV, Hata K, Ingraham HA
The Journal of neuroscience : the official journal of the Society for Neuroscience27(50)13624-34(2007 Dec)

The G protein-coupled receptor repertoires of human and mouse.
Vassilatis DK, Hohmann JG, Zeng H, Li F, Ranchalis JE, Mortrud MT, Brown A, Rodriguez SS, Weller JR, Wright AC, Bergmann JE, Gaitanaris GA
Proceedings of the National Academy of Sciences of the United States of America100(8)4903-8(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Gpr149 null mice are one of the few models with enhanced fertility.
Title: Deletion of the novel oocyte-enriched gene, Gpr149, leads to increased fertility in mice.

The following Gpr149 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gpr149 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu31805	NM_001356525.1 Latest version!	Mus musculus G protein-coupled receptor 149 (Gpr149), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu31805	XM_006501322.3 Latest version!	Mus musculus G protein-coupled receptor 149 (Gpr149), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu06315	NM_177346.5 Latest version!	Mus musculus G protein-coupled receptor 149 (Gpr149), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$433.30 $619.00
OMu73457	XM_011240076.2 Latest version!	Mus musculus G protein-coupled receptor 149 (Gpr149), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu06315	NM_177346.4	Mus musculus G protein-coupled receptor 149 (Gpr149), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$433.30 $619.00
OMu73457	XM_011240076.1	Mus musculus G protein-coupled receptor 149 (Gpr149), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gpr149 ( NM_001356525.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001343454.1 Mus musculus G protein-coupled receptor 149 (Gpr149), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu31805
Clone ID Related Accession (Same CDS sequence)	XM_006501322.3 , NM_001356525.1
Accession Version	NM_001356525.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1740bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Mus musculus(house mouse)
Product	probable G-protein coupled receptor 149 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC115721.11 and AC114424.10. On Oct 7, 2017 this sequence version replaced XM_006501322.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660821.53349.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTTTCT TTCTCAGCAA CTTAACAAAT GACTCTAACC TGTGGAAAGC AAGTCATAAT 
TCTACGGAGA CGGACCTTAT GAATTCACCA GCAACTCTGA CTCTTTCTCT CTTTTGCCTG 
ATCTGTATAA TGACTCTCGC AGCTCTGGTG GGCAGCATTT TCTCACTGGT TTCGCTGCTG 
ACCATGCAAT ACAGAACTGT TTTGTCCATA CTTGTGACAT CTTGGTCTGT GGATGATCTC 
TTGAGCGTCT TGTCCGTGGC CATCTTCATG GTTTTACAGT GGCCAAAAGA GGCACAGGGC 
TACTTCCAGT CTCTGTGCAC CACCTCTGCC CTACTGTATA TGTGCCAGGG CCTCTCCAGC 
AACTTGAAGG CGACTCTCAT AGTCTGCTAC AACTTTTATA CGATGAACAG GACGGTGGAG 
AGTCAGTCAA GCTCCTGGCG ATTAGGACAG GTACTTGGTG TGACTTTAAC CGTTTGGGCA 
GTCAGCCTGC TGCTGGCTTC GCTCCCGCTC TGTGGCTGGG GTGTCTTCGT GCGCACGCCT 
TGGGGCTGCC TGACCGACTG CTCCAGCCCA TACGTGCTGC TCCTCTTCGC GGTATACGCT 
TCCGCTTTCG GGCTCCTGGC GGTTCTCTCT GTCCCTCTCA CTCACCAGCT GCTGTGTTCG 
GAGGAACCGC TGAGACTCCA TGCCAACTAC CAGGAAATTT CTCGTGGCGC CTCTACTCCT 
GGGACCCCTG CTGCTGCGGG AAGAGTGCTC TGTCTGCCGC CAGAGGATGT TGAGATCCCT 
GCTCTGCGAT GCACTGGGGG ATGCTCCCCG AGCTCCGACG TGGTGTTTGC CCCGGGTCAG 
CCGGCTGCCT CCGGCGCTGG AGCCGGCAGG CGAGAGAATC CCGGGACTCC CCAGGGCACC 
AACAGCTTCC CCTTGAGCTT GGCACAGAAG CGCTTTTCTT TGATTCTAGC GCTGACGAAA 
GTCATCCTTT GGCTACCCAT GATGATCCAC ATGGTGGTAA AACACGTGGT GGGGTTTCAG 
AGCCTTCCGG TCGATATGCT CAGCTTTCTA CTAAGCCTGC TGGCCAGCAG TGTAACACCA 
GTGTTTGTCT TATCCAAACG TTGGGCCCAC TTGCCTTGTG GCTGCATCAT CAACTGCCAG 
CCAGACACCT ACTCCGTTGC CTTCGATGGG AAAAAGTCGA AGAGGAAAGG CTTTGAATTC 
AATCTATCAT TCCAACAAAG TTATGGACTC TATAAAATAC CACATGCAGA TTGCTATGAT 
GATGATGAAA ATTCCATATC CTATCACAAC CCAAAGAATT ATGAGTGTGA AGCTACAAAG 
GAGCCTTGGG GAGACAACCG CAGTGTCTTC AATACCATCA CAGTAGAAAT CAGCACCACG 
CCACCGCTGG ACAGCGCCAC ACTGACGGGT GTCCACAAGT GTACCAATAC TGACATCCCA 
GAATCCAAAC AGGCCATGAA AGAGGAAAAG GGTGCTTTTT CTGTTAAAAC AGAAAGCGAT 
ATTAACTATG GAGAAACCAC CTCTTTTGAA GGTCCAGAGA GACGACTGTC CCATGAGGAG 
AATCAGAAAC CAGATCTTTC AGACTGGGAA TGGTGCAGGA GTAAGTCTGA ACGAACGCCT 
CGCCAGATTA CTAATCCTAT AAAATCACAG AATCTATTTA TCCATGAAGA CATGGAGCTT 
GAGACAGTGC AGAGCCAAAC AGACTCACAT ATCTGTTGCG GTCAAATGTA TGACCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001343454.1
CDS	865..2604
Translation	MSFFLSNLTNDSNLWKASHNSTETDLMNSPATLTLSLFCLICIMTLAALVGSIFSLVSLLTMQYRTVLSILVTSWSVDDLLSVLSVAIFMVLQWPKEAQGYFQSLCTTSALLYMCQGLSSNLKATLIVCYNFYTMNRTVESQSSSWRLGQVLGVTLTVWAVSLLLASLPLCGWGVFVRTPWGCLTDCSSPYVLLLFAVYASAFGLLAVLSVPLTHQLLCSEEPLRLHANYQEISRGASTPGTPAAAGRVLCLPPEDVEIPALRCTGGCSPSSDVVFAPGQPAASGAGAGRRENPGTPQGTNSFPLSLAQKRFSLILALTKVILWLPMMIHMVVKHVVGFQSLPVDMLSFLLSLLASSVTPVFVLSKRWAHLPCGCIINCQPDTYSVAFDGKKSKRKGFEFNLSFQQSYGLYKIPHADCYDDDENSISYHNPKNYECEATKEPWGDNRSVFNTITVEISTTPPLDSATLTGVHKCTNTDIPESKQAMKEEKGAFSVKTESDINYGETTSFEGPERRLSHEENQKPDLSDWEWCRSKSERTPRQITNPIKSQNLFIHEDMELETVQSQTDSHICCGQMYDP

Target ORF information:

RefSeq Version	NM_001356525.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus G protein-coupled receptor 149 (Gpr149), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001356525.1

ORF Insert Sequence:

ATGTCTTTCT TTCTCAGCAA CTTAACAAAT GACTCTAACC TGTGGAAAGC AAGTCATAAT 
TCTACGGAGA CGGACCTTAT GAATTCACCA GCAACTCTGA CTCTTTCTCT CTTTTGCCTG 
ATCTGTATAA TGACTCTCGC AGCTCTGGTG GGCAGCATTT TCTCACTGGT TTCGCTGCTG 
ACCATGCAAT ACAGAACTGT TTTGTCCATA CTTGTGACAT CTTGGTCTGT GGATGATCTC 
TTGAGCGTCT TGTCCGTGGC CATCTTCATG GTTTTACAGT GGCCAAAAGA GGCACAGGGC 
TACTTCCAGT CTCTGTGCAC CACCTCTGCC CTACTGTATA TGTGCCAGGG CCTCTCCAGC 
AACTTGAAGG CGACTCTCAT AGTCTGCTAC AACTTTTATA CGATGAACAG GACGGTGGAG 
AGTCAGTCAA GCTCCTGGCG ATTAGGACAG GTACTTGGTG TGACTTTAAC CGTTTGGGCA 
GTCAGCCTGC TGCTGGCTTC GCTCCCGCTC TGTGGCTGGG GTGTCTTCGT GCGCACGCCT 
TGGGGCTGCC TGACCGACTG CTCCAGCCCA TACGTGCTGC TCCTCTTCGC GGTATACGCT 
TCCGCTTTCG GGCTCCTGGC GGTTCTCTCT GTCCCTCTCA CTCACCAGCT GCTGTGTTCG 
GAGGAACCGC TGAGACTCCA TGCCAACTAC CAGGAAATTT CTCGTGGCGC CTCTACTCCT 
GGGACCCCTG CTGCTGCGGG AAGAGTGCTC TGTCTGCCGC CAGAGGATGT TGAGATCCCT 
GCTCTGCGAT GCACTGGGGG ATGCTCCCCG AGCTCCGACG TGGTGTTTGC CCCGGGTCAG 
CCGGCTGCCT CCGGCGCTGG AGCCGGCAGG CGAGAGAATC CCGGGACTCC CCAGGGCACC 
AACAGCTTCC CCTTGAGCTT GGCACAGAAG CGCTTTTCTT TGATTCTAGC GCTGACGAAA 
GTCATCCTTT GGCTACCCAT GATGATCCAC ATGGTGGTAA AACACGTGGT GGGGTTTCAG 
AGCCTTCCGG TCGATATGCT CAGCTTTCTA CTAAGCCTGC TGGCCAGCAG TGTAACACCA 
GTGTTTGTCT TATCCAAACG TTGGGCCCAC TTGCCTTGTG GCTGCATCAT CAACTGCCAG 
CCAGACACCT ACTCCGTTGC CTTCGATGGG AAAAAGTCGA AGAGGAAAGG CTTTGAATTC 
AATCTATCAT TCCAACAAAG TTATGGACTC TATAAAATAC CACATGCAGA TTGCTATGAT 
GATGATGAAA ATTCCATATC CTATCACAAC CCAAAGAATT ATGAGTGTGA AGCTACAAAG 
GAGCCTTGGG GAGACAACCG CAGTGTCTTC AATACCATCA CAGTAGAAAT CAGCACCACG 
CCACCGCTGG ACAGCGCCAC ACTGACGGGT GTCCACAAGT GTACCAATAC TGACATCCCA 
GAATCCAAAC AGGCCATGAA AGAGGAAAAG GGTGCTTTTT CTGTTAAAAC AGAAAGCGAT 
ATTAACTATG GAGAAACCAC CTCTTTTGAA GGTCCAGAGA GACGACTGTC CCATGAGGAG 
AATCAGAAAC CAGATCTTTC AGACTGGGAA TGGTGCAGGA GTAAGTCTGA ACGAACGCCT 
CGCCAGATTA CTAATCCTAT AAAATCACAG AATCTATTTA TCCATGAAGA CATGGAGCTT 
GAGACAGTGC AGAGCCAAAC AGACTCACAT ATCTGTTGCG GTCAAATGTA TGACCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gpr149 ( XM_006501322.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501385.1 Mus musculus G protein-coupled receptor 149 (Gpr149), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu31805
Clone ID Related Accession (Same CDS sequence)	XM_006501322.3 , NM_001356525.1
Accession Version	XM_006501322.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1740bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	probable G-protein coupled receptor 149 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006501322.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTTCT TTCTCAGCAA CTTAACAAAT GACTCTAACC TGTGGAAAGC AAGTCATAAT 
TCTACGGAGA CGGACCTTAT GAATTCACCA GCAACTCTGA CTCTTTCTCT CTTTTGCCTG 
ATCTGTATAA TGACTCTCGC AGCTCTGGTG GGCAGCATTT TCTCACTGGT TTCGCTGCTG 
ACCATGCAAT ACAGAACTGT TTTGTCCATA CTTGTGACAT CTTGGTCTGT GGATGATCTC 
TTGAGCGTCT TGTCCGTGGC CATCTTCATG GTTTTACAGT GGCCAAAAGA GGCACAGGGC 
TACTTCCAGT CTCTGTGCAC CACCTCTGCC CTACTGTATA TGTGCCAGGG CCTCTCCAGC 
AACTTGAAGG CGACTCTCAT AGTCTGCTAC AACTTTTATA CGATGAACAG GACGGTGGAG 
AGTCAGTCAA GCTCCTGGCG ATTAGGACAG GTACTTGGTG TGACTTTAAC CGTTTGGGCA 
GTCAGCCTGC TGCTGGCTTC GCTCCCGCTC TGTGGCTGGG GTGTCTTCGT GCGCACGCCT 
TGGGGCTGCC TGACCGACTG CTCCAGCCCA TACGTGCTGC TCCTCTTCGC GGTATACGCT 
TCCGCTTTCG GGCTCCTGGC GGTTCTCTCT GTCCCTCTCA CTCACCAGCT GCTGTGTTCG 
GAGGAACCGC TGAGACTCCA TGCCAACTAC CAGGAAATTT CTCGTGGCGC CTCTACTCCT 
GGGACCCCTG CTGCTGCGGG AAGAGTGCTC TGTCTGCCGC CAGAGGATGT TGAGATCCCT 
GCTCTGCGAT GCACTGGGGG ATGCTCCCCG AGCTCCGACG TGGTGTTTGC CCCGGGTCAG 
CCGGCTGCCT CCGGCGCTGG AGCCGGCAGG CGAGAGAATC CCGGGACTCC CCAGGGCACC 
AACAGCTTCC CCTTGAGCTT GGCACAGAAG CGCTTTTCTT TGATTCTAGC GCTGACGAAA 
GTCATCCTTT GGCTACCCAT GATGATCCAC ATGGTGGTAA AACACGTGGT GGGGTTTCAG 
AGCCTTCCGG TCGATATGCT CAGCTTTCTA CTAAGCCTGC TGGCCAGCAG TGTAACACCA 
GTGTTTGTCT TATCCAAACG TTGGGCCCAC TTGCCTTGTG GCTGCATCAT CAACTGCCAG 
CCAGACACCT ACTCCGTTGC CTTCGATGGG AAAAAGTCGA AGAGGAAAGG CTTTGAATTC 
AATCTATCAT TCCAACAAAG TTATGGACTC TATAAAATAC CACATGCAGA TTGCTATGAT 
GATGATGAAA ATTCCATATC CTATCACAAC CCAAAGAATT ATGAGTGTGA AGCTACAAAG 
GAGCCTTGGG GAGACAACCG CAGTGTCTTC AATACCATCA CAGTAGAAAT CAGCACCACG 
CCACCGCTGG ACAGCGCCAC ACTGACGGGT GTCCACAAGT GTACCAATAC TGACATCCCA 
GAATCCAAAC AGGCCATGAA AGAGGAAAAG GGTGCTTTTT CTGTTAAAAC AGAAAGCGAT 
ATTAACTATG GAGAAACCAC CTCTTTTGAA GGTCCAGAGA GACGACTGTC CCATGAGGAG 
AATCAGAAAC CAGATCTTTC AGACTGGGAA TGGTGCAGGA GTAAGTCTGA ACGAACGCCT 
CGCCAGATTA CTAATCCTAT AAAATCACAG AATCTATTTA TCCATGAAGA CATGGAGCTT 
GAGACAGTGC AGAGCCAAAC AGACTCACAT ATCTGTTGCG GTCAAATGTA TGACCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501385.1
CDS	867..2606
Translation	MSFFLSNLTNDSNLWKASHNSTETDLMNSPATLTLSLFCLICIMTLAALVGSIFSLVSLLTMQYRTVLSILVTSWSVDDLLSVLSVAIFMVLQWPKEAQGYFQSLCTTSALLYMCQGLSSNLKATLIVCYNFYTMNRTVESQSSSWRLGQVLGVTLTVWAVSLLLASLPLCGWGVFVRTPWGCLTDCSSPYVLLLFAVYASAFGLLAVLSVPLTHQLLCSEEPLRLHANYQEISRGASTPGTPAAAGRVLCLPPEDVEIPALRCTGGCSPSSDVVFAPGQPAASGAGAGRRENPGTPQGTNSFPLSLAQKRFSLILALTKVILWLPMMIHMVVKHVVGFQSLPVDMLSFLLSLLASSVTPVFVLSKRWAHLPCGCIINCQPDTYSVAFDGKKSKRKGFEFNLSFQQSYGLYKIPHADCYDDDENSISYHNPKNYECEATKEPWGDNRSVFNTITVEISTTPPLDSATLTGVHKCTNTDIPESKQAMKEEKGAFSVKTESDINYGETTSFEGPERRLSHEENQKPDLSDWEWCRSKSERTPRQITNPIKSQNLFIHEDMELETVQSQTDSHICCGQMYDP

Target ORF information:

RefSeq Version	XM_006501322.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus G protein-coupled receptor 149 (Gpr149), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501322.3

ORF Insert Sequence:

ATGTCTTTCT TTCTCAGCAA CTTAACAAAT GACTCTAACC TGTGGAAAGC AAGTCATAAT 
TCTACGGAGA CGGACCTTAT GAATTCACCA GCAACTCTGA CTCTTTCTCT CTTTTGCCTG 
ATCTGTATAA TGACTCTCGC AGCTCTGGTG GGCAGCATTT TCTCACTGGT TTCGCTGCTG 
ACCATGCAAT ACAGAACTGT TTTGTCCATA CTTGTGACAT CTTGGTCTGT GGATGATCTC 
TTGAGCGTCT TGTCCGTGGC CATCTTCATG GTTTTACAGT GGCCAAAAGA GGCACAGGGC 
TACTTCCAGT CTCTGTGCAC CACCTCTGCC CTACTGTATA TGTGCCAGGG CCTCTCCAGC 
AACTTGAAGG CGACTCTCAT AGTCTGCTAC AACTTTTATA CGATGAACAG GACGGTGGAG 
AGTCAGTCAA GCTCCTGGCG ATTAGGACAG GTACTTGGTG TGACTTTAAC CGTTTGGGCA 
GTCAGCCTGC TGCTGGCTTC GCTCCCGCTC TGTGGCTGGG GTGTCTTCGT GCGCACGCCT 
TGGGGCTGCC TGACCGACTG CTCCAGCCCA TACGTGCTGC TCCTCTTCGC GGTATACGCT 
TCCGCTTTCG GGCTCCTGGC GGTTCTCTCT GTCCCTCTCA CTCACCAGCT GCTGTGTTCG 
GAGGAACCGC TGAGACTCCA TGCCAACTAC CAGGAAATTT CTCGTGGCGC CTCTACTCCT 
GGGACCCCTG CTGCTGCGGG AAGAGTGCTC TGTCTGCCGC CAGAGGATGT TGAGATCCCT 
GCTCTGCGAT GCACTGGGGG ATGCTCCCCG AGCTCCGACG TGGTGTTTGC CCCGGGTCAG 
CCGGCTGCCT CCGGCGCTGG AGCCGGCAGG CGAGAGAATC CCGGGACTCC CCAGGGCACC 
AACAGCTTCC CCTTGAGCTT GGCACAGAAG CGCTTTTCTT TGATTCTAGC GCTGACGAAA 
GTCATCCTTT GGCTACCCAT GATGATCCAC ATGGTGGTAA AACACGTGGT GGGGTTTCAG 
AGCCTTCCGG TCGATATGCT CAGCTTTCTA CTAAGCCTGC TGGCCAGCAG TGTAACACCA 
GTGTTTGTCT TATCCAAACG TTGGGCCCAC TTGCCTTGTG GCTGCATCAT CAACTGCCAG 
CCAGACACCT ACTCCGTTGC CTTCGATGGG AAAAAGTCGA AGAGGAAAGG CTTTGAATTC 
AATCTATCAT TCCAACAAAG TTATGGACTC TATAAAATAC CACATGCAGA TTGCTATGAT 
GATGATGAAA ATTCCATATC CTATCACAAC CCAAAGAATT ATGAGTGTGA AGCTACAAAG 
GAGCCTTGGG GAGACAACCG CAGTGTCTTC AATACCATCA CAGTAGAAAT CAGCACCACG 
CCACCGCTGG ACAGCGCCAC ACTGACGGGT GTCCACAAGT GTACCAATAC TGACATCCCA 
GAATCCAAAC AGGCCATGAA AGAGGAAAAG GGTGCTTTTT CTGTTAAAAC AGAAAGCGAT 
ATTAACTATG GAGAAACCAC CTCTTTTGAA GGTCCAGAGA GACGACTGTC CCATGAGGAG 
AATCAGAAAC CAGATCTTTC AGACTGGGAA TGGTGCAGGA GTAAGTCTGA ACGAACGCCT 
CGCCAGATTA CTAATCCTAT AAAATCACAG AATCTATTTA TCCATGAAGA CATGGAGCTT 
GAGACAGTGC AGAGCCAAAC AGACTCACAT ATCTGTTGCG GTCAAATGTA TGACCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gpr149 ( XM_011240076.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011238378.1 Mus musculus G protein-coupled receptor 149 (Gpr149), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu73457
Clone ID Related Accession (Same CDS sequence)	XM_011240076.1 , XM_011240076.2
Accession Version	XM_011240076.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	probable G-protein coupled receptor 149 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTTCT TTCTCAGCAA CTTAACAAAT GACTCTAACC TGTGGAAAGC AAGTCATAAT 
TCTACGGAGA CGGACCTTAT GAATTCACCA GCAACTCTGA CTCTTTCTCT CTTTTGCCTG 
ATCTGTATAA TGACTCTCGC AGCTCTGGTG GGCAGCATTT TCTCACTGGT TTCGCTGCTG 
ACCATGCAAT ACAGAACTGT TTTGTCCATA CTTGTGACAT CTTGGTCTGT GGATGATCTC 
TTGAGCGTCT TGTCCGTGGC CATCTTCATG GTTTTACAGT GGCCAAAAGA GGCACAGGGC 
TACTTCCAGT CTCTGTGCAC CACCTCTGCC CTACTGTATA TGTGCCAGGG CCTCTCCAGC 
AACTTGAAGG CGACTCTCAT AGTCTGCTAC AACTTTTATA CGATGAACAG GACGGTGGAG 
AGTCAGTCAA GCTCCTGGCG ATTAGGACAG GTACTTGGTG TGACTTTAAC CGTTTGGGCA 
GTCAGCCTGC TGCTGGCTTC GCTCCCGCTC TGTGGCTGGG GTGTCTTCGT GCGCACGCCT 
TGGGGCTGCC TGACCGACTG CTCCAGCCCA TACGTGCTGC TCCTCTTCGC GGTATACGCT 
TCCGCTTTCG GGCTCCTGGC GGTTCTCTCT GTCCCTCTCA CTCACCAGCT GCTGTGTTCG 
GAGGAACCGC TGAGACTCCA TGCCAACTAC CAGGAAATTT CTCGTGGCGC CTCTACTCCT 
GGGACCCCTG CTGCTGCGGG AAGAGTGCTC TGTCTGCCGC CAGAGGATGT TGAGATCCCT 
GCTCTGCGAT GCACTGGGGG ATGCTCCCCG AGCTCCGACG TGGTGTTTGC CCCGGGTCAG 
CCGGCTGCCT CCGGCGCTGG AGCCGGCAGG CGAGAGAATC CCGGGACTCC CCAGGGCACC 
AACAGCTTCC CCTTGAGCTT GGCACAGAAG CGCTTTTCTT TGATTCTAGC GCTGACGAAA 
GTCATCCTTT GGCTACCCAT GATGATCCAC ATGGTGGTAA AACACGTGGT GGGGTTTCAG 
AGCCTTCCGG TCGATATGCT CAGCTTTCTA CTAAGCCTGC TGGCCAGCAG TGTAACACCA 
GTGTTTGTCT TATCCAAACG TTGGGCCCAC TTGCCTTGTG GCTGCATCAT CAACTGCCAG 
CCAGACACCT ACTCCGTTGC CTTCGATGGG AAAAAGTCGA AGAGGAAAGG CTTTGAATTC 
AATCTATCAT TCCAACAAAG TTATGGACTC TATAAAATAC CACATGCAGA TTGCTATGAT 
GATGATGAAA ATTCCATATC CTATCACAAC CCAAAGAATT ATGAGTGTGA AGCTACAAAG 
GAGCCTTGGG GAGACAACCG CAGTGTCTTC AATACCATCA CAGTAGAAAT CAGCACCACG 
CCACCGCTGG ACAGCGCCAC ACTGACGGGT GTCCACAAGT GTACCAATAC TGACATCCCA 
GAATCCAAAC AGGCCATGAA AGAGGAAAAG GGTGCTTTTT CTGTTAAAAC AGAAAGCGAT 
ATTAACTATG GAGAAACCAC CTCTTTTGAA GGTCCAGAGA GACGACTGTC CCATGAGGAG 
AATCAGAAAC CAGATCTTTC AGACTGGGAA TGGTGCAGGA GTAAGTCTGA ACGAACGCCT 
CGCCAGGTAC TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011238378.1
CDS	865..2499
Translation	MSFFLSNLTNDSNLWKASHNSTETDLMNSPATLTLSLFCLICIMTLAALVGSIFSLVSLLTMQYRTVLSILVTSWSVDDLLSVLSVAIFMVLQWPKEAQGYFQSLCTTSALLYMCQGLSSNLKATLIVCYNFYTMNRTVESQSSSWRLGQVLGVTLTVWAVSLLLASLPLCGWGVFVRTPWGCLTDCSSPYVLLLFAVYASAFGLLAVLSVPLTHQLLCSEEPLRLHANYQEISRGASTPGTPAAAGRVLCLPPEDVEIPALRCTGGCSPSSDVVFAPGQPAASGAGAGRRENPGTPQGTNSFPLSLAQKRFSLILALTKVILWLPMMIHMVVKHVVGFQSLPVDMLSFLLSLLASSVTPVFVLSKRWAHLPCGCIINCQPDTYSVAFDGKKSKRKGFEFNLSFQQSYGLYKIPHADCYDDDENSISYHNPKNYECEATKEPWGDNRSVFNTITVEISTTPPLDSATLTGVHKCTNTDIPESKQAMKEEKGAFSVKTESDINYGETTSFEGPERRLSHEENQKPDLSDWEWCRSKSERTPRQVL

Target ORF information:

RefSeq Version	XM_011240076.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus G protein-coupled receptor 149 (Gpr149), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240076.1

ORF Insert Sequence:

ATGTCTTTCT TTCTCAGCAA CTTAACAAAT GACTCTAACC TGTGGAAAGC AAGTCATAAT 
TCTACGGAGA CGGACCTTAT GAATTCACCA GCAACTCTGA CTCTTTCTCT CTTTTGCCTG 
ATCTGTATAA TGACTCTCGC AGCTCTGGTG GGCAGCATTT TCTCACTGGT TTCGCTGCTG 
ACCATGCAAT ACAGAACTGT TTTGTCCATA CTTGTGACAT CTTGGTCTGT GGATGATCTC 
TTGAGCGTCT TGTCCGTGGC CATCTTCATG GTTTTACAGT GGCCAAAAGA GGCACAGGGC 
TACTTCCAGT CTCTGTGCAC CACCTCTGCC CTACTGTATA TGTGCCAGGG CCTCTCCAGC 
AACTTGAAGG CGACTCTCAT AGTCTGCTAC AACTTTTATA CGATGAACAG GACGGTGGAG 
AGTCAGTCAA GCTCCTGGCG ATTAGGACAG GTACTTGGTG TGACTTTAAC CGTTTGGGCA 
GTCAGCCTGC TGCTGGCTTC GCTCCCGCTC TGTGGCTGGG GTGTCTTCGT GCGCACGCCT 
TGGGGCTGCC TGACCGACTG CTCCAGCCCA TACGTGCTGC TCCTCTTCGC GGTATACGCT 
TCCGCTTTCG GGCTCCTGGC GGTTCTCTCT GTCCCTCTCA CTCACCAGCT GCTGTGTTCG 
GAGGAACCGC TGAGACTCCA TGCCAACTAC CAGGAAATTT CTCGTGGCGC CTCTACTCCT 
GGGACCCCTG CTGCTGCGGG AAGAGTGCTC TGTCTGCCGC CAGAGGATGT TGAGATCCCT 
GCTCTGCGAT GCACTGGGGG ATGCTCCCCG AGCTCCGACG TGGTGTTTGC CCCGGGTCAG 
CCGGCTGCCT CCGGCGCTGG AGCCGGCAGG CGAGAGAATC CCGGGACTCC CCAGGGCACC 
AACAGCTTCC CCTTGAGCTT GGCACAGAAG CGCTTTTCTT TGATTCTAGC GCTGACGAAA 
GTCATCCTTT GGCTACCCAT GATGATCCAC ATGGTGGTAA AACACGTGGT GGGGTTTCAG 
AGCCTTCCGG TCGATATGCT CAGCTTTCTA CTAAGCCTGC TGGCCAGCAG TGTAACACCA 
GTGTTTGTCT TATCCAAACG TTGGGCCCAC TTGCCTTGTG GCTGCATCAT CAACTGCCAG 
CCAGACACCT ACTCCGTTGC CTTCGATGGG AAAAAGTCGA AGAGGAAAGG CTTTGAATTC 
AATCTATCAT TCCAACAAAG TTATGGACTC TATAAAATAC CACATGCAGA TTGCTATGAT 
GATGATGAAA ATTCCATATC CTATCACAAC CCAAAGAATT ATGAGTGTGA AGCTACAAAG 
GAGCCTTGGG GAGACAACCG CAGTGTCTTC AATACCATCA CAGTAGAAAT CAGCACCACG 
CCACCGCTGG ACAGCGCCAC ACTGACGGGT GTCCACAAGT GTACCAATAC TGACATCCCA 
GAATCCAAAC AGGCCATGAA AGAGGAAAAG GGTGCTTTTT CTGTTAAAAC AGAAAGCGAT 
ATTAACTATG GAGAAACCAC CTCTTTTGAA GGTCCAGAGA GACGACTGTC CCATGAGGAG 
AATCAGAAAC CAGATCTTTC AGACTGGGAA TGGTGCAGGA GTAAGTCTGA ACGAACGCCT 
CGCCAGGTAC TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gpr149 ( NM_177346.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_796320.2 Mus musculus G protein-coupled receptor 149 (Gpr149), transcript variant 1, mRNA.

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CloneID	OMu06315
Clone ID Related Accession (Same CDS sequence)	NM_177346.5 , NM_177346.4
Accession Version	NM_177346.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2199bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Mus musculus(house mouse)
Product	probable G-protein coupled receptor 149 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC115721.11 and AC114424.10. On Oct 7, 2017 this sequence version replaced NM_177346.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660815.7771.1, AK136826.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTTTCT TTCTCAGCAA CTTAACAAAT GACTCTAACC TGTGGAAAGC AAGTCATAAT 
TCTACGGAGA CGGACCTTAT GAATTCACCA GCAACTCTGA CTCTTTCTCT CTTTTGCCTG 
ATCTGTATAA TGACTCTCGC AGCTCTGGTG GGCAGCATTT TCTCACTGGT TTCGCTGCTG 
ACCATGCAAT ACAGAACTGT TTTGTCCATA CTTGTGACAT CTTGGTCTGT GGATGATCTC 
TTGAGCGTCT TGTCCGTGGC CATCTTCATG GTTTTACAGT GGCCAAAAGA GGCACAGGGC 
TACTTCCAGT CTCTGTGCAC CACCTCTGCC CTACTGTATA TGTGCCAGGG CCTCTCCAGC 
AACTTGAAGG CGACTCTCAT AGTCTGCTAC AACTTTTATA CGATGAACAG GACGGTGGAG 
AGTCAGTCAA GCTCCTGGCG ATTAGGACAG GTACTTGGTG TGACTTTAAC CGTTTGGGCA 
GTCAGCCTGC TGCTGGCTTC GCTCCCGCTC TGTGGCTGGG GTGTCTTCGT GCGCACGCCT 
TGGGGCTGCC TGACCGACTG CTCCAGCCCA TACGTGCTGC TCCTCTTCGC GGTATACGCT 
TCCGCTTTCG GGCTCCTGGC GGTTCTCTCT GTCCCTCTCA CTCACCAGCT GCTGTGTTCG 
GAGGAACCGC TGAGACTCCA TGCCAACTAC CAGGAAATTT CTCGTGGCGC CTCTACTCCT 
GGGACCCCTG CTGCTGCGGG AAGAGTGCTC TGTCTGCCGC CAGAGGATGT TGAGATCCCT 
GCTCTGCGAT GCACTGGGGG ATGCTCCCCG AGCTCCGACG TGGTGTTTGC CCCGGGTCAG 
CCGGCTGCCT CCGGCGCTGG AGCCGGCAGG CGAGAGAATC CCGGGACTCC CCAGGGCACC 
AACAGCTTCC CCTTGAGCTT GGCACAGAAG CGCTTTTCTT TGATTCTAGC GCTGACGAAA 
GTCATCCTTT GGCTACCCAT GATGATCCAC ATGGTGGTAA AACACGTGGT GGGGTTTCAG 
AGCCTTCCGG TCGATATGCT CAGCTTTCTA CTAAGCCTGC TGGCCAGCAG TGTAACACCA 
GTGTTTGTCT TATCCAAACG TTGGGCCCAC TTGCCTTGTG GCTGCATCAT CAACTGCCAG 
CCAGACACCT ACTCCGTTGC CTTCGATGGG AAAAAGTCGA AGAGGAAAGG CTTTGAATTC 
AATCTATCAT TCCAACAAAG TTATGGACTC TATAAAATAC CACATGCAGA TTGCTATGAT 
GATGATGAAA ATTCCATATC CTATCACAAC CCAAAGAATT ATGAGTGTGA AGCTACAAAG 
GAGCCTTGGG GAGACAACCG CAGTGTCTTC AATACCATCA CAGTAGAAAT CAGCACCACG 
CCACCGCTGG ACAGCGCCAC ACTGACGGGT GTCCACAAGT GTACCAATAC TGACATCCCA 
GAATCCAAAC AGGCCATGAA AGAGGAAAAG GGTGCTTTTT CTGTTAAAAC AGAAAGCGAT 
ATTAACTATG GAGAAACCAC CTCTTTTGAA GGTCCAGAGA GACGACTGTC CCATGAGGAG 
AATCAGAAAC CAGATCTTTC AGACTGGGAA TGGTGCAGGA GTAAGTCTGA ACGAACGCCT 
CGCCAGCGTT CCGGTGGTGG GCTGGCTATT CCCATATGCG CGTTCCAGGG GACTGTGTCT 
CTCCAAGCAC CTACTGGGAA AACGCTCTCT CTTTCTACTT ATGAGGTCAG CGCAGAAGGG 
CAAAAGATAA CCCCAGCCTC TAAGAAAATA GAAGTCTATC GATCTAAAAG TGTTGGCCAT 
GAACCGAACT CAGAGGAGTC TCCCTCCACG TTTGCGGACA CCAGTGTAAA AATACACTTG 
GAAGTTCTTG AAATTTGTGA CAACGATGAG GCTCTAGACA CTGTGTCAAT TATCAGCAAC 
ATCAGCCAAT CCTCCACAAA AGTCAGGTCT CCTTCTCTGC GCTACTCACG GAAGGAAAAC 
AGATTCGTCT CCTGCGACTT AGGAGAAACT GCCTCATACT CGCTCTTTTT ACCCACAAGT 
GATCCTGATG GTGATATTAA CATCTCTATC CCTGACACGG TGGAGGCCCA CAGACAGAAC 
AGCAGGCGGC AGCATGAAGA GAGGGATGGC TACCAGGAAG AGATCCAGTT GTTAAACAAA 
GCCTACAGGA AAAGAGAGGC AGAAAGCAAG GGTGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_796320.2
CDS	565..2763
Translation	MSFFLSNLTNDSNLWKASHNSTETDLMNSPATLTLSLFCLICIMTLAALVGSIFSLVSLLTMQYRTVLSILVTSWSVDDLLSVLSVAIFMVLQWPKEAQGYFQSLCTTSALLYMCQGLSSNLKATLIVCYNFYTMNRTVESQSSSWRLGQVLGVTLTVWAVSLLLASLPLCGWGVFVRTPWGCLTDCSSPYVLLLFAVYASAFGLLAVLSVPLTHQLLCSEEPLRLHANYQEISRGASTPGTPAAAGRVLCLPPEDVEIPALRCTGGCSPSSDVVFAPGQPAASGAGAGRRENPGTPQGTNSFPLSLAQKRFSLILALTKVILWLPMMIHMVVKHVVGFQSLPVDMLSFLLSLLASSVTPVFVLSKRWAHLPCGCIINCQPDTYSVAFDGKKSKRKGFEFNLSFQQSYGLYKIPHADCYDDDENSISYHNPKNYECEATKEPWGDNRSVFNTITVEISTTPPLDSATLTGVHKCTNTDIPESKQAMKEEKGAFSVKTESDINYGETTSFEGPERRLSHEENQKPDLSDWEWCRSKSERTPRQRSGGGLAIPICAFQGTVSLQAPTGKTLSLSTYEVSAEGQKITPASKKIEVYRSKSVGHEPNSEESPSTFADTSVKIHLEVLEICDNDEALDTVSIISNISQSSTKVRSPSLRYSRKENRFVSCDLGETASYSLFLPTSDPDGDINISIPDTVEAHRQNSRRQHEERDGYQEEIQLLNKAYRKREAESKGD

Target ORF information:

RefSeq Version	NM_177346.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus G protein-coupled receptor 149 (Gpr149), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177346.5

ORF Insert Sequence:

ATGTCTTTCT TTCTCAGCAA CTTAACAAAT GACTCTAACC TGTGGAAAGC AAGTCATAAT 
TCTACGGAGA CGGACCTTAT GAATTCACCA GCAACTCTGA CTCTTTCTCT CTTTTGCCTG 
ATCTGTATAA TGACTCTCGC AGCTCTGGTG GGCAGCATTT TCTCACTGGT TTCGCTGCTG 
ACCATGCAAT ACAGAACTGT TTTGTCCATA CTTGTGACAT CTTGGTCTGT GGATGATCTC 
TTGAGCGTCT TGTCCGTGGC CATCTTCATG GTTTTACAGT GGCCAAAAGA GGCACAGGGC 
TACTTCCAGT CTCTGTGCAC CACCTCTGCC CTACTGTATA TGTGCCAGGG CCTCTCCAGC 
AACTTGAAGG CGACTCTCAT AGTCTGCTAC AACTTTTATA CGATGAACAG GACGGTGGAG 
AGTCAGTCAA GCTCCTGGCG ATTAGGACAG GTACTTGGTG TGACTTTAAC CGTTTGGGCA 
GTCAGCCTGC TGCTGGCTTC GCTCCCGCTC TGTGGCTGGG GTGTCTTCGT GCGCACGCCT 
TGGGGCTGCC TGACCGACTG CTCCAGCCCA TACGTGCTGC TCCTCTTCGC GGTATACGCT 
TCCGCTTTCG GGCTCCTGGC GGTTCTCTCT GTCCCTCTCA CTCACCAGCT GCTGTGTTCG 
GAGGAACCGC TGAGACTCCA TGCCAACTAC CAGGAAATTT CTCGTGGCGC CTCTACTCCT 
GGGACCCCTG CTGCTGCGGG AAGAGTGCTC TGTCTGCCGC CAGAGGATGT TGAGATCCCT 
GCTCTGCGAT GCACTGGGGG ATGCTCCCCG AGCTCCGACG TGGTGTTTGC CCCGGGTCAG 
CCGGCTGCCT CCGGCGCTGG AGCCGGCAGG CGAGAGAATC CCGGGACTCC CCAGGGCACC 
AACAGCTTCC CCTTGAGCTT GGCACAGAAG CGCTTTTCTT TGATTCTAGC GCTGACGAAA 
GTCATCCTTT GGCTACCCAT GATGATCCAC ATGGTGGTAA AACACGTGGT GGGGTTTCAG 
AGCCTTCCGG TCGATATGCT CAGCTTTCTA CTAAGCCTGC TGGCCAGCAG TGTAACACCA 
GTGTTTGTCT TATCCAAACG TTGGGCCCAC TTGCCTTGTG GCTGCATCAT CAACTGCCAG 
CCAGACACCT ACTCCGTTGC CTTCGATGGG AAAAAGTCGA AGAGGAAAGG CTTTGAATTC 
AATCTATCAT TCCAACAAAG TTATGGACTC TATAAAATAC CACATGCAGA TTGCTATGAT 
GATGATGAAA ATTCCATATC CTATCACAAC CCAAAGAATT ATGAGTGTGA AGCTACAAAG 
GAGCCTTGGG GAGACAACCG CAGTGTCTTC AATACCATCA CAGTAGAAAT CAGCACCACG 
CCACCGCTGG ACAGCGCCAC ACTGACGGGT GTCCACAAGT GTACCAATAC TGACATCCCA 
GAATCCAAAC AGGCCATGAA AGAGGAAAAG GGTGCTTTTT CTGTTAAAAC AGAAAGCGAT 
ATTAACTATG GAGAAACCAC CTCTTTTGAA GGTCCAGAGA GACGACTGTC CCATGAGGAG 
AATCAGAAAC CAGATCTTTC AGACTGGGAA TGGTGCAGGA GTAAGTCTGA ACGAACGCCT 
CGCCAGCGTT CCGGTGGTGG GCTGGCTATT CCCATATGCG CGTTCCAGGG GACTGTGTCT 
CTCCAAGCAC CTACTGGGAA AACGCTCTCT CTTTCTACTT ATGAGGTCAG CGCAGAAGGG 
CAAAAGATAA CCCCAGCCTC TAAGAAAATA GAAGTCTATC GATCTAAAAG TGTTGGCCAT 
GAACCGAACT CAGAGGAGTC TCCCTCCACG TTTGCGGACA CCAGTGTAAA AATACACTTG 
GAAGTTCTTG AAATTTGTGA CAACGATGAG GCTCTAGACA CTGTGTCAAT TATCAGCAAC 
ATCAGCCAAT CCTCCACAAA AGTCAGGTCT CCTTCTCTGC GCTACTCACG GAAGGAAAAC 
AGATTCGTCT CCTGCGACTT AGGAGAAACT GCCTCATACT CGCTCTTTTT ACCCACAAGT 
GATCCTGATG GTGATATTAA CATCTCTATC CCTGACACGG TGGAGGCCCA CAGACAGAAC 
AGCAGGCGGC AGCATGAAGA GAGGGATGGC TACCAGGAAG AGATCCAGTT GTTAAACAAA 
GCCTACAGGA AAAGAGAGGC AGAAAGCAAG GGTGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gpr149 ( NM_177346.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_796320.2 Mus musculus G protein-coupled receptor 149 (Gpr149), mRNA.

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CloneID	OMu06315
Clone ID Related Accession (Same CDS sequence)	NM_177346.5 , NM_177346.4
Accession Version	NM_177346.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2199bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-12
Organism	Mus musculus(House mouse)
Product	probable G-protein coupled receptor 149
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC115721.11 and AC114424.10. On May 2, 2007 this sequence version replaced NM_177346.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK136826.1, AK166029.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END##

ATGTCTTTCT TTCTCAGCAA CTTAACAAAT GACTCTAACC TGTGGAAAGC AAGTCATAAT 
TCTACGGAGA CGGACCTTAT GAATTCACCA GCAACTCTGA CTCTTTCTCT CTTTTGCCTG 
ATCTGTATAA TGACTCTCGC AGCTCTGGTG GGCAGCATTT TCTCACTGGT TTCGCTGCTG 
ACCATGCAAT ACAGAACTGT TTTGTCCATA CTTGTGACAT CTTGGTCTGT GGATGATCTC 
TTGAGCGTCT TGTCCGTGGC CATCTTCATG GTTTTACAGT GGCCAAAAGA GGCACAGGGC 
TACTTCCAGT CTCTGTGCAC CACCTCTGCC CTACTGTATA TGTGCCAGGG CCTCTCCAGC 
AACTTGAAGG CGACTCTCAT AGTCTGCTAC AACTTTTATA CGATGAACAG GACGGTGGAG 
AGTCAGTCAA GCTCCTGGCG ATTAGGACAG GTACTTGGTG TGACTTTAAC CGTTTGGGCA 
GTCAGCCTGC TGCTGGCTTC GCTCCCGCTC TGTGGCTGGG GTGTCTTCGT GCGCACGCCT 
TGGGGCTGCC TGACCGACTG CTCCAGCCCA TACGTGCTGC TCCTCTTCGC GGTATACGCT 
TCCGCTTTCG GGCTCCTGGC GGTTCTCTCT GTCCCTCTCA CTCACCAGCT GCTGTGTTCG 
GAGGAACCGC TGAGACTCCA TGCCAACTAC CAGGAAATTT CTCGTGGCGC CTCTACTCCT 
GGGACCCCTG CTGCTGCGGG AAGAGTGCTC TGTCTGCCGC CAGAGGATGT TGAGATCCCT 
GCTCTGCGAT GCACTGGGGG ATGCTCCCCG AGCTCCGACG TGGTGTTTGC CCCGGGTCAG 
CCGGCTGCCT CCGGCGCTGG AGCCGGCAGG CGAGAGAATC CCGGGACTCC CCAGGGCACC 
AACAGCTTCC CCTTGAGCTT GGCACAGAAG CGCTTTTCTT TGATTCTAGC GCTGACGAAA 
GTCATCCTTT GGCTACCCAT GATGATCCAC ATGGTGGTAA AACACGTGGT GGGGTTTCAG 
AGCCTTCCGG TCGATATGCT CAGCTTTCTA CTAAGCCTGC TGGCCAGCAG TGTAACACCA 
GTGTTTGTCT TATCCAAACG TTGGGCCCAC TTGCCTTGTG GCTGCATCAT CAACTGCCAG 
CCAGACACCT ACTCCGTTGC CTTCGATGGG AAAAAGTCGA AGAGGAAAGG CTTTGAATTC 
AATCTATCAT TCCAACAAAG TTATGGACTC TATAAAATAC CACATGCAGA TTGCTATGAT 
GATGATGAAA ATTCCATATC CTATCACAAC CCAAAGAATT ATGAGTGTGA AGCTACAAAG 
GAGCCTTGGG GAGACAACCG CAGTGTCTTC AATACCATCA CAGTAGAAAT CAGCACCACG 
CCACCGCTGG ACAGCGCCAC ACTGACGGGT GTCCACAAGT GTACCAATAC TGACATCCCA 
GAATCCAAAC AGGCCATGAA AGAGGAAAAG GGTGCTTTTT CTGTTAAAAC AGAAAGCGAT 
ATTAACTATG GAGAAACCAC CTCTTTTGAA GGTCCAGAGA GACGACTGTC CCATGAGGAG 
AATCAGAAAC CAGATCTTTC AGACTGGGAA TGGTGCAGGA GTAAGTCTGA ACGAACGCCT 
CGCCAGCGTT CCGGTGGTGG GCTGGCTATT CCCATATGCG CGTTCCAGGG GACTGTGTCT 
CTCCAAGCAC CTACTGGGAA AACGCTCTCT CTTTCTACTT ATGAGGTCAG CGCAGAAGGG 
CAAAAGATAA CCCCAGCCTC TAAGAAAATA GAAGTCTATC GATCTAAAAG TGTTGGCCAT 
GAACCGAACT CAGAGGAGTC TCCCTCCACG TTTGCGGACA CCAGTGTAAA AATACACTTG 
GAAGTTCTTG AAATTTGTGA CAACGATGAG GCTCTAGACA CTGTGTCAAT TATCAGCAAC 
ATCAGCCAAT CCTCCACAAA AGTCAGGTCT CCTTCTCTGC GCTACTCACG GAAGGAAAAC 
AGATTCGTCT CCTGCGACTT AGGAGAAACT GCCTCATACT CGCTCTTTTT ACCCACAAGT 
GATCCTGATG GTGATATTAA CATCTCTATC CCTGACACGG TGGAGGCCCA CAGACAGAAC 
AGCAGGCGGC AGCATGAAGA GAGGGATGGC TACCAGGAAG AGATCCAGTT GTTAAACAAA 
GCCTACAGGA AAAGAGAGGC AGAAAGCAAG GGTGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_796320.2
CDS	565..2763
Translation	MSFFLSNLTNDSNLWKASHNSTETDLMNSPATLTLSLFCLICIMTLAALVGSIFSLVSLLTMQYRTVLSILVTSWSVDDLLSVLSVAIFMVLQWPKEAQGYFQSLCTTSALLYMCQGLSSNLKATLIVCYNFYTMNRTVESQSSSWRLGQVLGVTLTVWAVSLLLASLPLCGWGVFVRTPWGCLTDCSSPYVLLLFAVYASAFGLLAVLSVPLTHQLLCSEEPLRLHANYQEISRGASTPGTPAAAGRVLCLPPEDVEIPALRCTGGCSPSSDVVFAPGQPAASGAGAGRRENPGTPQGTNSFPLSLAQKRFSLILALTKVILWLPMMIHMVVKHVVGFQSLPVDMLSFLLSLLASSVTPVFVLSKRWAHLPCGCIINCQPDTYSVAFDGKKSKRKGFEFNLSFQQSYGLYKIPHADCYDDDENSISYHNPKNYECEATKEPWGDNRSVFNTITVEISTTPPLDSATLTGVHKCTNTDIPESKQAMKEEKGAFSVKTESDINYGETTSFEGPERRLSHEENQKPDLSDWEWCRSKSERTPRQRSGGGLAIPICAFQGTVSLQAPTGKTLSLSTYEVSAEGQKITPASKKIEVYRSKSVGHEPNSEESPSTFADTSVKIHLEVLEICDNDEALDTVSIISNISQSSTKVRSPSLRYSRKENRFVSCDLGETASYSLFLPTSDPDGDINISIPDTVEAHRQNSRRQHEERDGYQEEIQLLNKAYRKREAESKGD

Target ORF information:

RefSeq Version	NM_177346.4
Organism	Mus musculus(House mouse)
Definition	Mus musculus G protein-coupled receptor 149 (Gpr149), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177346.4

ORF Insert Sequence:

ATGTCTTTCT TTCTCAGCAA CTTAACAAAT GACTCTAACC TGTGGAAAGC AAGTCATAAT 
TCTACGGAGA CGGACCTTAT GAATTCACCA GCAACTCTGA CTCTTTCTCT CTTTTGCCTG 
ATCTGTATAA TGACTCTCGC AGCTCTGGTG GGCAGCATTT TCTCACTGGT TTCGCTGCTG 
ACCATGCAAT ACAGAACTGT TTTGTCCATA CTTGTGACAT CTTGGTCTGT GGATGATCTC 
TTGAGCGTCT TGTCCGTGGC CATCTTCATG GTTTTACAGT GGCCAAAAGA GGCACAGGGC 
TACTTCCAGT CTCTGTGCAC CACCTCTGCC CTACTGTATA TGTGCCAGGG CCTCTCCAGC 
AACTTGAAGG CGACTCTCAT AGTCTGCTAC AACTTTTATA CGATGAACAG GACGGTGGAG 
AGTCAGTCAA GCTCCTGGCG ATTAGGACAG GTACTTGGTG TGACTTTAAC CGTTTGGGCA 
GTCAGCCTGC TGCTGGCTTC GCTCCCGCTC TGTGGCTGGG GTGTCTTCGT GCGCACGCCT 
TGGGGCTGCC TGACCGACTG CTCCAGCCCA TACGTGCTGC TCCTCTTCGC GGTATACGCT 
TCCGCTTTCG GGCTCCTGGC GGTTCTCTCT GTCCCTCTCA CTCACCAGCT GCTGTGTTCG 
GAGGAACCGC TGAGACTCCA TGCCAACTAC CAGGAAATTT CTCGTGGCGC CTCTACTCCT 
GGGACCCCTG CTGCTGCGGG AAGAGTGCTC TGTCTGCCGC CAGAGGATGT TGAGATCCCT 
GCTCTGCGAT GCACTGGGGG ATGCTCCCCG AGCTCCGACG TGGTGTTTGC CCCGGGTCAG 
CCGGCTGCCT CCGGCGCTGG AGCCGGCAGG CGAGAGAATC CCGGGACTCC CCAGGGCACC 
AACAGCTTCC CCTTGAGCTT GGCACAGAAG CGCTTTTCTT TGATTCTAGC GCTGACGAAA 
GTCATCCTTT GGCTACCCAT GATGATCCAC ATGGTGGTAA AACACGTGGT GGGGTTTCAG 
AGCCTTCCGG TCGATATGCT CAGCTTTCTA CTAAGCCTGC TGGCCAGCAG TGTAACACCA 
GTGTTTGTCT TATCCAAACG TTGGGCCCAC TTGCCTTGTG GCTGCATCAT CAACTGCCAG 
CCAGACACCT ACTCCGTTGC CTTCGATGGG AAAAAGTCGA AGAGGAAAGG CTTTGAATTC 
AATCTATCAT TCCAACAAAG TTATGGACTC TATAAAATAC CACATGCAGA TTGCTATGAT 
GATGATGAAA ATTCCATATC CTATCACAAC CCAAAGAATT ATGAGTGTGA AGCTACAAAG 
GAGCCTTGGG GAGACAACCG CAGTGTCTTC AATACCATCA CAGTAGAAAT CAGCACCACG 
CCACCGCTGG ACAGCGCCAC ACTGACGGGT GTCCACAAGT GTACCAATAC TGACATCCCA 
GAATCCAAAC AGGCCATGAA AGAGGAAAAG GGTGCTTTTT CTGTTAAAAC AGAAAGCGAT 
ATTAACTATG GAGAAACCAC CTCTTTTGAA GGTCCAGAGA GACGACTGTC CCATGAGGAG 
AATCAGAAAC CAGATCTTTC AGACTGGGAA TGGTGCAGGA GTAAGTCTGA ACGAACGCCT 
CGCCAGCGTT CCGGTGGTGG GCTGGCTATT CCCATATGCG CGTTCCAGGG GACTGTGTCT 
CTCCAAGCAC CTACTGGGAA AACGCTCTCT CTTTCTACTT ATGAGGTCAG CGCAGAAGGG 
CAAAAGATAA CCCCAGCCTC TAAGAAAATA GAAGTCTATC GATCTAAAAG TGTTGGCCAT 
GAACCGAACT CAGAGGAGTC TCCCTCCACG TTTGCGGACA CCAGTGTAAA AATACACTTG 
GAAGTTCTTG AAATTTGTGA CAACGATGAG GCTCTAGACA CTGTGTCAAT TATCAGCAAC 
ATCAGCCAAT CCTCCACAAA AGTCAGGTCT CCTTCTCTGC GCTACTCACG GAAGGAAAAC 
AGATTCGTCT CCTGCGACTT AGGAGAAACT GCCTCATACT CGCTCTTTTT ACCCACAAGT 
GATCCTGATG GTGATATTAA CATCTCTATC CCTGACACGG TGGAGGCCCA CAGACAGAAC 
AGCAGGCGGC AGCATGAAGA GAGGGATGGC TACCAGGAAG AGATCCAGTT GTTAAACAAA 
GCCTACAGGA AAAGAGAGGC AGAAAGCAAG GGTGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gpr149 ( XM_011240076.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011238378.1 Mus musculus G protein-coupled receptor 149 (Gpr149), transcript variant X1, mRNA.

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CloneID	OMu73457
Clone ID Related Accession (Same CDS sequence)	XM_011240076.1 , XM_011240076.2
Accession Version	XM_011240076.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	probable G-protein coupled receptor 149 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011240076.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTTCT TTCTCAGCAA CTTAACAAAT GACTCTAACC TGTGGAAAGC AAGTCATAAT 
TCTACGGAGA CGGACCTTAT GAATTCACCA GCAACTCTGA CTCTTTCTCT CTTTTGCCTG 
ATCTGTATAA TGACTCTCGC AGCTCTGGTG GGCAGCATTT TCTCACTGGT TTCGCTGCTG 
ACCATGCAAT ACAGAACTGT TTTGTCCATA CTTGTGACAT CTTGGTCTGT GGATGATCTC 
TTGAGCGTCT TGTCCGTGGC CATCTTCATG GTTTTACAGT GGCCAAAAGA GGCACAGGGC 
TACTTCCAGT CTCTGTGCAC CACCTCTGCC CTACTGTATA TGTGCCAGGG CCTCTCCAGC 
AACTTGAAGG CGACTCTCAT AGTCTGCTAC AACTTTTATA CGATGAACAG GACGGTGGAG 
AGTCAGTCAA GCTCCTGGCG ATTAGGACAG GTACTTGGTG TGACTTTAAC CGTTTGGGCA 
GTCAGCCTGC TGCTGGCTTC GCTCCCGCTC TGTGGCTGGG GTGTCTTCGT GCGCACGCCT 
TGGGGCTGCC TGACCGACTG CTCCAGCCCA TACGTGCTGC TCCTCTTCGC GGTATACGCT 
TCCGCTTTCG GGCTCCTGGC GGTTCTCTCT GTCCCTCTCA CTCACCAGCT GCTGTGTTCG 
GAGGAACCGC TGAGACTCCA TGCCAACTAC CAGGAAATTT CTCGTGGCGC CTCTACTCCT 
GGGACCCCTG CTGCTGCGGG AAGAGTGCTC TGTCTGCCGC CAGAGGATGT TGAGATCCCT 
GCTCTGCGAT GCACTGGGGG ATGCTCCCCG AGCTCCGACG TGGTGTTTGC CCCGGGTCAG 
CCGGCTGCCT CCGGCGCTGG AGCCGGCAGG CGAGAGAATC CCGGGACTCC CCAGGGCACC 
AACAGCTTCC CCTTGAGCTT GGCACAGAAG CGCTTTTCTT TGATTCTAGC GCTGACGAAA 
GTCATCCTTT GGCTACCCAT GATGATCCAC ATGGTGGTAA AACACGTGGT GGGGTTTCAG 
AGCCTTCCGG TCGATATGCT CAGCTTTCTA CTAAGCCTGC TGGCCAGCAG TGTAACACCA 
GTGTTTGTCT TATCCAAACG TTGGGCCCAC TTGCCTTGTG GCTGCATCAT CAACTGCCAG 
CCAGACACCT ACTCCGTTGC CTTCGATGGG AAAAAGTCGA AGAGGAAAGG CTTTGAATTC 
AATCTATCAT TCCAACAAAG TTATGGACTC TATAAAATAC CACATGCAGA TTGCTATGAT 
GATGATGAAA ATTCCATATC CTATCACAAC CCAAAGAATT ATGAGTGTGA AGCTACAAAG 
GAGCCTTGGG GAGACAACCG CAGTGTCTTC AATACCATCA CAGTAGAAAT CAGCACCACG 
CCACCGCTGG ACAGCGCCAC ACTGACGGGT GTCCACAAGT GTACCAATAC TGACATCCCA 
GAATCCAAAC AGGCCATGAA AGAGGAAAAG GGTGCTTTTT CTGTTAAAAC AGAAAGCGAT 
ATTAACTATG GAGAAACCAC CTCTTTTGAA GGTCCAGAGA GACGACTGTC CCATGAGGAG 
AATCAGAAAC CAGATCTTTC AGACTGGGAA TGGTGCAGGA GTAAGTCTGA ACGAACGCCT 
CGCCAGGTAC TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011238378.1
CDS	626..2260
Translation	MSFFLSNLTNDSNLWKASHNSTETDLMNSPATLTLSLFCLICIMTLAALVGSIFSLVSLLTMQYRTVLSILVTSWSVDDLLSVLSVAIFMVLQWPKEAQGYFQSLCTTSALLYMCQGLSSNLKATLIVCYNFYTMNRTVESQSSSWRLGQVLGVTLTVWAVSLLLASLPLCGWGVFVRTPWGCLTDCSSPYVLLLFAVYASAFGLLAVLSVPLTHQLLCSEEPLRLHANYQEISRGASTPGTPAAAGRVLCLPPEDVEIPALRCTGGCSPSSDVVFAPGQPAASGAGAGRRENPGTPQGTNSFPLSLAQKRFSLILALTKVILWLPMMIHMVVKHVVGFQSLPVDMLSFLLSLLASSVTPVFVLSKRWAHLPCGCIINCQPDTYSVAFDGKKSKRKGFEFNLSFQQSYGLYKIPHADCYDDDENSISYHNPKNYECEATKEPWGDNRSVFNTITVEISTTPPLDSATLTGVHKCTNTDIPESKQAMKEEKGAFSVKTESDINYGETTSFEGPERRLSHEENQKPDLSDWEWCRSKSERTPRQVL

Target ORF information:

RefSeq Version	XM_011240076.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus G protein-coupled receptor 149 (Gpr149), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011240076.2

ORF Insert Sequence:

ATGTCTTTCT TTCTCAGCAA CTTAACAAAT GACTCTAACC TGTGGAAAGC AAGTCATAAT 
TCTACGGAGA CGGACCTTAT GAATTCACCA GCAACTCTGA CTCTTTCTCT CTTTTGCCTG 
ATCTGTATAA TGACTCTCGC AGCTCTGGTG GGCAGCATTT TCTCACTGGT TTCGCTGCTG 
ACCATGCAAT ACAGAACTGT TTTGTCCATA CTTGTGACAT CTTGGTCTGT GGATGATCTC 
TTGAGCGTCT TGTCCGTGGC CATCTTCATG GTTTTACAGT GGCCAAAAGA GGCACAGGGC 
TACTTCCAGT CTCTGTGCAC CACCTCTGCC CTACTGTATA TGTGCCAGGG CCTCTCCAGC 
AACTTGAAGG CGACTCTCAT AGTCTGCTAC AACTTTTATA CGATGAACAG GACGGTGGAG 
AGTCAGTCAA GCTCCTGGCG ATTAGGACAG GTACTTGGTG TGACTTTAAC CGTTTGGGCA 
GTCAGCCTGC TGCTGGCTTC GCTCCCGCTC TGTGGCTGGG GTGTCTTCGT GCGCACGCCT 
TGGGGCTGCC TGACCGACTG CTCCAGCCCA TACGTGCTGC TCCTCTTCGC GGTATACGCT 
TCCGCTTTCG GGCTCCTGGC GGTTCTCTCT GTCCCTCTCA CTCACCAGCT GCTGTGTTCG 
GAGGAACCGC TGAGACTCCA TGCCAACTAC CAGGAAATTT CTCGTGGCGC CTCTACTCCT 
GGGACCCCTG CTGCTGCGGG AAGAGTGCTC TGTCTGCCGC CAGAGGATGT TGAGATCCCT 
GCTCTGCGAT GCACTGGGGG ATGCTCCCCG AGCTCCGACG TGGTGTTTGC CCCGGGTCAG 
CCGGCTGCCT CCGGCGCTGG AGCCGGCAGG CGAGAGAATC CCGGGACTCC CCAGGGCACC 
AACAGCTTCC CCTTGAGCTT GGCACAGAAG CGCTTTTCTT TGATTCTAGC GCTGACGAAA 
GTCATCCTTT GGCTACCCAT GATGATCCAC ATGGTGGTAA AACACGTGGT GGGGTTTCAG 
AGCCTTCCGG TCGATATGCT CAGCTTTCTA CTAAGCCTGC TGGCCAGCAG TGTAACACCA 
GTGTTTGTCT TATCCAAACG TTGGGCCCAC TTGCCTTGTG GCTGCATCAT CAACTGCCAG 
CCAGACACCT ACTCCGTTGC CTTCGATGGG AAAAAGTCGA AGAGGAAAGG CTTTGAATTC 
AATCTATCAT TCCAACAAAG TTATGGACTC TATAAAATAC CACATGCAGA TTGCTATGAT 
GATGATGAAA ATTCCATATC CTATCACAAC CCAAAGAATT ATGAGTGTGA AGCTACAAAG 
GAGCCTTGGG GAGACAACCG CAGTGTCTTC AATACCATCA CAGTAGAAAT CAGCACCACG 
CCACCGCTGG ACAGCGCCAC ACTGACGGGT GTCCACAAGT GTACCAATAC TGACATCCCA 
GAATCCAAAC AGGCCATGAA AGAGGAAAAG GGTGCTTTTT CTGTTAAAAC AGAAAGCGAT 
ATTAACTATG GAGAAACCAC CTCTTTTGAA GGTCCAGAGA GACGACTGTC CCATGAGGAG 
AATCAGAAAC CAGATCTTTC AGACTGGGAA TGGTGCAGGA GTAAGTCTGA ACGAACGCCT 
CGCCAGGTAC TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Deletion of the novel oocyte-enriched gene, Gpr149, leads to increased fertility in mice.
Endocrinology151(1)358-68(2010 Jan)
Edson MA,Lin YN,Matzuk MM

The neonatal ventromedial hypothalamus transcriptome reveals novel markers with spatially distinct patterning.
The Journal of neuroscience : the official journal of the Society for Neuroscience27(50)13624-34(2007 Dec)
Kurrasch DM,Cheung CC,Lee FY,Tran PV,Hata K,Ingraham HA

The G protein-coupled receptor repertoires of human and mouse.
Proceedings of the National Academy of Sciences of the United States of America100(8)4903-8(2003 Apr)
Vassilatis DK,Hohmann JG,Zeng H,Li F,Ranchalis JE,Mortrud MT,Brown A,Rodriguez SS,Weller JR,Wright AC,Bergmann JE,Gaitanaris GA

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Gpr149 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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