Gpr149 cDNA ORF clone, Mus musculus(house mouse)

The following Gpr149 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gpr149 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu31805 NM_001356525.1
Latest version!
Mus musculus G protein-coupled receptor 149 (Gpr149), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OMu31805 XM_006501322.3
Latest version!
Mus musculus G protein-coupled receptor 149 (Gpr149), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OMu06315 NM_177346.5
Latest version!
Mus musculus G protein-coupled receptor 149 (Gpr149), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $433.30
$619.00
OMu73457 XM_011240076.2
Latest version!
Mus musculus G protein-coupled receptor 149 (Gpr149), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
View Old Accession Versions >>
OMu06315 NM_177346.4 Mus musculus G protein-coupled receptor 149 (Gpr149), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $433.30
$619.00
OMu73457 XM_011240076.1 Mus musculus G protein-coupled receptor 149 (Gpr149), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu31805
    Clone ID Related Accession (Same CDS sequence) XM_006501322.3 , NM_001356525.1
    Accession Version NM_001356525.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1740bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-19
    Organism Mus musculus(house mouse)
    Product probable G-protein coupled receptor 149 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC115721.11 and AC114424.10. On Oct 7, 2017 this sequence version replaced XM_006501322.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660821.53349.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGTCTTTCT TTCTCAGCAA CTTAACAAAT GACTCTAACC TGTGGAAAGC AAGTCATAAT 
    TCTACGGAGA CGGACCTTAT GAATTCACCA GCAACTCTGA CTCTTTCTCT CTTTTGCCTG
    ATCTGTATAA TGACTCTCGC AGCTCTGGTG GGCAGCATTT TCTCACTGGT TTCGCTGCTG
    ACCATGCAAT ACAGAACTGT TTTGTCCATA CTTGTGACAT CTTGGTCTGT GGATGATCTC
    TTGAGCGTCT TGTCCGTGGC CATCTTCATG GTTTTACAGT GGCCAAAAGA GGCACAGGGC
    TACTTCCAGT CTCTGTGCAC CACCTCTGCC CTACTGTATA TGTGCCAGGG CCTCTCCAGC
    AACTTGAAGG CGACTCTCAT AGTCTGCTAC AACTTTTATA CGATGAACAG GACGGTGGAG
    AGTCAGTCAA GCTCCTGGCG ATTAGGACAG GTACTTGGTG TGACTTTAAC CGTTTGGGCA
    GTCAGCCTGC TGCTGGCTTC GCTCCCGCTC TGTGGCTGGG GTGTCTTCGT GCGCACGCCT
    TGGGGCTGCC TGACCGACTG CTCCAGCCCA TACGTGCTGC TCCTCTTCGC GGTATACGCT
    TCCGCTTTCG GGCTCCTGGC GGTTCTCTCT GTCCCTCTCA CTCACCAGCT GCTGTGTTCG
    GAGGAACCGC TGAGACTCCA TGCCAACTAC CAGGAAATTT CTCGTGGCGC CTCTACTCCT
    GGGACCCCTG CTGCTGCGGG AAGAGTGCTC TGTCTGCCGC CAGAGGATGT TGAGATCCCT
    GCTCTGCGAT GCACTGGGGG ATGCTCCCCG AGCTCCGACG TGGTGTTTGC CCCGGGTCAG
    CCGGCTGCCT CCGGCGCTGG AGCCGGCAGG CGAGAGAATC CCGGGACTCC CCAGGGCACC
    AACAGCTTCC CCTTGAGCTT GGCACAGAAG CGCTTTTCTT TGATTCTAGC GCTGACGAAA
    GTCATCCTTT GGCTACCCAT GATGATCCAC ATGGTGGTAA AACACGTGGT GGGGTTTCAG
    AGCCTTCCGG TCGATATGCT CAGCTTTCTA CTAAGCCTGC TGGCCAGCAG TGTAACACCA
    GTGTTTGTCT TATCCAAACG TTGGGCCCAC TTGCCTTGTG GCTGCATCAT CAACTGCCAG
    CCAGACACCT ACTCCGTTGC CTTCGATGGG AAAAAGTCGA AGAGGAAAGG CTTTGAATTC
    AATCTATCAT TCCAACAAAG TTATGGACTC TATAAAATAC CACATGCAGA TTGCTATGAT
    GATGATGAAA ATTCCATATC CTATCACAAC CCAAAGAATT ATGAGTGTGA AGCTACAAAG
    GAGCCTTGGG GAGACAACCG CAGTGTCTTC AATACCATCA CAGTAGAAAT CAGCACCACG
    CCACCGCTGG ACAGCGCCAC ACTGACGGGT GTCCACAAGT GTACCAATAC TGACATCCCA
    GAATCCAAAC AGGCCATGAA AGAGGAAAAG GGTGCTTTTT CTGTTAAAAC AGAAAGCGAT
    ATTAACTATG GAGAAACCAC CTCTTTTGAA GGTCCAGAGA GACGACTGTC CCATGAGGAG
    AATCAGAAAC CAGATCTTTC AGACTGGGAA TGGTGCAGGA GTAAGTCTGA ACGAACGCCT
    CGCCAGATTA CTAATCCTAT AAAATCACAG AATCTATTTA TCCATGAAGA CATGGAGCTT
    GAGACAGTGC AGAGCCAAAC AGACTCACAT ATCTGTTGCG GTCAAATGTA TGACCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001343454.1
    CDS865..2604
    Translation

    Target ORF information:

    RefSeq Version NM_001356525.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus G protein-coupled receptor 149 (Gpr149), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001356525.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGTCTTTCT TTCTCAGCAA CTTAACAAAT GACTCTAACC TGTGGAAAGC AAGTCATAAT 
    TCTACGGAGA CGGACCTTAT GAATTCACCA GCAACTCTGA CTCTTTCTCT CTTTTGCCTG
    ATCTGTATAA TGACTCTCGC AGCTCTGGTG GGCAGCATTT TCTCACTGGT TTCGCTGCTG
    ACCATGCAAT ACAGAACTGT TTTGTCCATA CTTGTGACAT CTTGGTCTGT GGATGATCTC
    TTGAGCGTCT TGTCCGTGGC CATCTTCATG GTTTTACAGT GGCCAAAAGA GGCACAGGGC
    TACTTCCAGT CTCTGTGCAC CACCTCTGCC CTACTGTATA TGTGCCAGGG CCTCTCCAGC
    AACTTGAAGG CGACTCTCAT AGTCTGCTAC AACTTTTATA CGATGAACAG GACGGTGGAG
    AGTCAGTCAA GCTCCTGGCG ATTAGGACAG GTACTTGGTG TGACTTTAAC CGTTTGGGCA
    GTCAGCCTGC TGCTGGCTTC GCTCCCGCTC TGTGGCTGGG GTGTCTTCGT GCGCACGCCT
    TGGGGCTGCC TGACCGACTG CTCCAGCCCA TACGTGCTGC TCCTCTTCGC GGTATACGCT
    TCCGCTTTCG GGCTCCTGGC GGTTCTCTCT GTCCCTCTCA CTCACCAGCT GCTGTGTTCG
    GAGGAACCGC TGAGACTCCA TGCCAACTAC CAGGAAATTT CTCGTGGCGC CTCTACTCCT
    GGGACCCCTG CTGCTGCGGG AAGAGTGCTC TGTCTGCCGC CAGAGGATGT TGAGATCCCT
    GCTCTGCGAT GCACTGGGGG ATGCTCCCCG AGCTCCGACG TGGTGTTTGC CCCGGGTCAG
    CCGGCTGCCT CCGGCGCTGG AGCCGGCAGG CGAGAGAATC CCGGGACTCC CCAGGGCACC
    AACAGCTTCC CCTTGAGCTT GGCACAGAAG CGCTTTTCTT TGATTCTAGC GCTGACGAAA
    GTCATCCTTT GGCTACCCAT GATGATCCAC ATGGTGGTAA AACACGTGGT GGGGTTTCAG
    AGCCTTCCGG TCGATATGCT CAGCTTTCTA CTAAGCCTGC TGGCCAGCAG TGTAACACCA
    GTGTTTGTCT TATCCAAACG TTGGGCCCAC TTGCCTTGTG GCTGCATCAT CAACTGCCAG
    CCAGACACCT ACTCCGTTGC CTTCGATGGG AAAAAGTCGA AGAGGAAAGG CTTTGAATTC
    AATCTATCAT TCCAACAAAG TTATGGACTC TATAAAATAC CACATGCAGA TTGCTATGAT
    GATGATGAAA ATTCCATATC CTATCACAAC CCAAAGAATT ATGAGTGTGA AGCTACAAAG
    GAGCCTTGGG GAGACAACCG CAGTGTCTTC AATACCATCA CAGTAGAAAT CAGCACCACG
    CCACCGCTGG ACAGCGCCAC ACTGACGGGT GTCCACAAGT GTACCAATAC TGACATCCCA
    GAATCCAAAC AGGCCATGAA AGAGGAAAAG GGTGCTTTTT CTGTTAAAAC AGAAAGCGAT
    ATTAACTATG GAGAAACCAC CTCTTTTGAA GGTCCAGAGA GACGACTGTC CCATGAGGAG
    AATCAGAAAC CAGATCTTTC AGACTGGGAA TGGTGCAGGA GTAAGTCTGA ACGAACGCCT
    CGCCAGATTA CTAATCCTAT AAAATCACAG AATCTATTTA TCCATGAAGA CATGGAGCTT
    GAGACAGTGC AGAGCCAAAC AGACTCACAT ATCTGTTGCG GTCAAATGTA TGACCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu31805
    Clone ID Related Accession (Same CDS sequence) XM_006501322.3 , NM_001356525.1
    Accession Version XM_006501322.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1740bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(House mouse)
    Product probable G-protein coupled receptor 149 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006501322.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGTCTTTCT TTCTCAGCAA CTTAACAAAT GACTCTAACC TGTGGAAAGC AAGTCATAAT 
    TCTACGGAGA CGGACCTTAT GAATTCACCA GCAACTCTGA CTCTTTCTCT CTTTTGCCTG
    ATCTGTATAA TGACTCTCGC AGCTCTGGTG GGCAGCATTT TCTCACTGGT TTCGCTGCTG
    ACCATGCAAT ACAGAACTGT TTTGTCCATA CTTGTGACAT CTTGGTCTGT GGATGATCTC
    TTGAGCGTCT TGTCCGTGGC CATCTTCATG GTTTTACAGT GGCCAAAAGA GGCACAGGGC
    TACTTCCAGT CTCTGTGCAC CACCTCTGCC CTACTGTATA TGTGCCAGGG CCTCTCCAGC
    AACTTGAAGG CGACTCTCAT AGTCTGCTAC AACTTTTATA CGATGAACAG GACGGTGGAG
    AGTCAGTCAA GCTCCTGGCG ATTAGGACAG GTACTTGGTG TGACTTTAAC CGTTTGGGCA
    GTCAGCCTGC TGCTGGCTTC GCTCCCGCTC TGTGGCTGGG GTGTCTTCGT GCGCACGCCT
    TGGGGCTGCC TGACCGACTG CTCCAGCCCA TACGTGCTGC TCCTCTTCGC GGTATACGCT
    TCCGCTTTCG GGCTCCTGGC GGTTCTCTCT GTCCCTCTCA CTCACCAGCT GCTGTGTTCG
    GAGGAACCGC TGAGACTCCA TGCCAACTAC CAGGAAATTT CTCGTGGCGC CTCTACTCCT
    GGGACCCCTG CTGCTGCGGG AAGAGTGCTC TGTCTGCCGC CAGAGGATGT TGAGATCCCT
    GCTCTGCGAT GCACTGGGGG ATGCTCCCCG AGCTCCGACG TGGTGTTTGC CCCGGGTCAG
    CCGGCTGCCT CCGGCGCTGG AGCCGGCAGG CGAGAGAATC CCGGGACTCC CCAGGGCACC
    AACAGCTTCC CCTTGAGCTT GGCACAGAAG CGCTTTTCTT TGATTCTAGC GCTGACGAAA
    GTCATCCTTT GGCTACCCAT GATGATCCAC ATGGTGGTAA AACACGTGGT GGGGTTTCAG
    AGCCTTCCGG TCGATATGCT CAGCTTTCTA CTAAGCCTGC TGGCCAGCAG TGTAACACCA
    GTGTTTGTCT TATCCAAACG TTGGGCCCAC TTGCCTTGTG GCTGCATCAT CAACTGCCAG
    CCAGACACCT ACTCCGTTGC CTTCGATGGG AAAAAGTCGA AGAGGAAAGG CTTTGAATTC
    AATCTATCAT TCCAACAAAG TTATGGACTC TATAAAATAC CACATGCAGA TTGCTATGAT
    GATGATGAAA ATTCCATATC CTATCACAAC CCAAAGAATT ATGAGTGTGA AGCTACAAAG
    GAGCCTTGGG GAGACAACCG CAGTGTCTTC AATACCATCA CAGTAGAAAT CAGCACCACG
    CCACCGCTGG ACAGCGCCAC ACTGACGGGT GTCCACAAGT GTACCAATAC TGACATCCCA
    GAATCCAAAC AGGCCATGAA AGAGGAAAAG GGTGCTTTTT CTGTTAAAAC AGAAAGCGAT
    ATTAACTATG GAGAAACCAC CTCTTTTGAA GGTCCAGAGA GACGACTGTC CCATGAGGAG
    AATCAGAAAC CAGATCTTTC AGACTGGGAA TGGTGCAGGA GTAAGTCTGA ACGAACGCCT
    CGCCAGATTA CTAATCCTAT AAAATCACAG AATCTATTTA TCCATGAAGA CATGGAGCTT
    GAGACAGTGC AGAGCCAAAC AGACTCACAT ATCTGTTGCG GTCAAATGTA TGACCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006501385.1
    CDS867..2606
    Translation

    Target ORF information:

    RefSeq Version XM_006501322.3
    Organism Mus musculus(House mouse)
    Definition Mus musculus G protein-coupled receptor 149 (Gpr149), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006501322.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGTCTTTCT TTCTCAGCAA CTTAACAAAT GACTCTAACC TGTGGAAAGC AAGTCATAAT 
    TCTACGGAGA CGGACCTTAT GAATTCACCA GCAACTCTGA CTCTTTCTCT CTTTTGCCTG
    ATCTGTATAA TGACTCTCGC AGCTCTGGTG GGCAGCATTT TCTCACTGGT TTCGCTGCTG
    ACCATGCAAT ACAGAACTGT TTTGTCCATA CTTGTGACAT CTTGGTCTGT GGATGATCTC
    TTGAGCGTCT TGTCCGTGGC CATCTTCATG GTTTTACAGT GGCCAAAAGA GGCACAGGGC
    TACTTCCAGT CTCTGTGCAC CACCTCTGCC CTACTGTATA TGTGCCAGGG CCTCTCCAGC
    AACTTGAAGG CGACTCTCAT AGTCTGCTAC AACTTTTATA CGATGAACAG GACGGTGGAG
    AGTCAGTCAA GCTCCTGGCG ATTAGGACAG GTACTTGGTG TGACTTTAAC CGTTTGGGCA
    GTCAGCCTGC TGCTGGCTTC GCTCCCGCTC TGTGGCTGGG GTGTCTTCGT GCGCACGCCT
    TGGGGCTGCC TGACCGACTG CTCCAGCCCA TACGTGCTGC TCCTCTTCGC GGTATACGCT
    TCCGCTTTCG GGCTCCTGGC GGTTCTCTCT GTCCCTCTCA CTCACCAGCT GCTGTGTTCG
    GAGGAACCGC TGAGACTCCA TGCCAACTAC CAGGAAATTT CTCGTGGCGC CTCTACTCCT
    GGGACCCCTG CTGCTGCGGG AAGAGTGCTC TGTCTGCCGC CAGAGGATGT TGAGATCCCT
    GCTCTGCGAT GCACTGGGGG ATGCTCCCCG AGCTCCGACG TGGTGTTTGC CCCGGGTCAG
    CCGGCTGCCT CCGGCGCTGG AGCCGGCAGG CGAGAGAATC CCGGGACTCC CCAGGGCACC
    AACAGCTTCC CCTTGAGCTT GGCACAGAAG CGCTTTTCTT TGATTCTAGC GCTGACGAAA
    GTCATCCTTT GGCTACCCAT GATGATCCAC ATGGTGGTAA AACACGTGGT GGGGTTTCAG
    AGCCTTCCGG TCGATATGCT CAGCTTTCTA CTAAGCCTGC TGGCCAGCAG TGTAACACCA
    GTGTTTGTCT TATCCAAACG TTGGGCCCAC TTGCCTTGTG GCTGCATCAT CAACTGCCAG
    CCAGACACCT ACTCCGTTGC CTTCGATGGG AAAAAGTCGA AGAGGAAAGG CTTTGAATTC
    AATCTATCAT TCCAACAAAG TTATGGACTC TATAAAATAC CACATGCAGA TTGCTATGAT
    GATGATGAAA ATTCCATATC CTATCACAAC CCAAAGAATT ATGAGTGTGA AGCTACAAAG
    GAGCCTTGGG GAGACAACCG CAGTGTCTTC AATACCATCA CAGTAGAAAT CAGCACCACG
    CCACCGCTGG ACAGCGCCAC ACTGACGGGT GTCCACAAGT GTACCAATAC TGACATCCCA
    GAATCCAAAC AGGCCATGAA AGAGGAAAAG GGTGCTTTTT CTGTTAAAAC AGAAAGCGAT
    ATTAACTATG GAGAAACCAC CTCTTTTGAA GGTCCAGAGA GACGACTGTC CCATGAGGAG
    AATCAGAAAC CAGATCTTTC AGACTGGGAA TGGTGCAGGA GTAAGTCTGA ACGAACGCCT
    CGCCAGATTA CTAATCCTAT AAAATCACAG AATCTATTTA TCCATGAAGA CATGGAGCTT
    GAGACAGTGC AGAGCCAAAC AGACTCACAT ATCTGTTGCG GTCAAATGTA TGACCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu73457
    Clone ID Related Accession (Same CDS sequence) XM_011240076.1 , XM_011240076.2
    Accession Version XM_011240076.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1635bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product probable G-protein coupled receptor 149 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGTCTTTCT TTCTCAGCAA CTTAACAAAT GACTCTAACC TGTGGAAAGC AAGTCATAAT 
    TCTACGGAGA CGGACCTTAT GAATTCACCA GCAACTCTGA CTCTTTCTCT CTTTTGCCTG
    ATCTGTATAA TGACTCTCGC AGCTCTGGTG GGCAGCATTT TCTCACTGGT TTCGCTGCTG
    ACCATGCAAT ACAGAACTGT TTTGTCCATA CTTGTGACAT CTTGGTCTGT GGATGATCTC
    TTGAGCGTCT TGTCCGTGGC CATCTTCATG GTTTTACAGT GGCCAAAAGA GGCACAGGGC
    TACTTCCAGT CTCTGTGCAC CACCTCTGCC CTACTGTATA TGTGCCAGGG CCTCTCCAGC
    AACTTGAAGG CGACTCTCAT AGTCTGCTAC AACTTTTATA CGATGAACAG GACGGTGGAG
    AGTCAGTCAA GCTCCTGGCG ATTAGGACAG GTACTTGGTG TGACTTTAAC CGTTTGGGCA
    GTCAGCCTGC TGCTGGCTTC GCTCCCGCTC TGTGGCTGGG GTGTCTTCGT GCGCACGCCT
    TGGGGCTGCC TGACCGACTG CTCCAGCCCA TACGTGCTGC TCCTCTTCGC GGTATACGCT
    TCCGCTTTCG GGCTCCTGGC GGTTCTCTCT GTCCCTCTCA CTCACCAGCT GCTGTGTTCG
    GAGGAACCGC TGAGACTCCA TGCCAACTAC CAGGAAATTT CTCGTGGCGC CTCTACTCCT
    GGGACCCCTG CTGCTGCGGG AAGAGTGCTC TGTCTGCCGC CAGAGGATGT TGAGATCCCT
    GCTCTGCGAT GCACTGGGGG ATGCTCCCCG AGCTCCGACG TGGTGTTTGC CCCGGGTCAG
    CCGGCTGCCT CCGGCGCTGG AGCCGGCAGG CGAGAGAATC CCGGGACTCC CCAGGGCACC
    AACAGCTTCC CCTTGAGCTT GGCACAGAAG CGCTTTTCTT TGATTCTAGC GCTGACGAAA
    GTCATCCTTT GGCTACCCAT GATGATCCAC ATGGTGGTAA AACACGTGGT GGGGTTTCAG
    AGCCTTCCGG TCGATATGCT CAGCTTTCTA CTAAGCCTGC TGGCCAGCAG TGTAACACCA
    GTGTTTGTCT TATCCAAACG TTGGGCCCAC TTGCCTTGTG GCTGCATCAT CAACTGCCAG
    CCAGACACCT ACTCCGTTGC CTTCGATGGG AAAAAGTCGA AGAGGAAAGG CTTTGAATTC
    AATCTATCAT TCCAACAAAG TTATGGACTC TATAAAATAC CACATGCAGA TTGCTATGAT
    GATGATGAAA ATTCCATATC CTATCACAAC CCAAAGAATT ATGAGTGTGA AGCTACAAAG
    GAGCCTTGGG GAGACAACCG CAGTGTCTTC AATACCATCA CAGTAGAAAT CAGCACCACG
    CCACCGCTGG ACAGCGCCAC ACTGACGGGT GTCCACAAGT GTACCAATAC TGACATCCCA
    GAATCCAAAC AGGCCATGAA AGAGGAAAAG GGTGCTTTTT CTGTTAAAAC AGAAAGCGAT
    ATTAACTATG GAGAAACCAC CTCTTTTGAA GGTCCAGAGA GACGACTGTC CCATGAGGAG
    AATCAGAAAC CAGATCTTTC AGACTGGGAA TGGTGCAGGA GTAAGTCTGA ACGAACGCCT
    CGCCAGGTAC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011238378.1
    CDS865..2499
    Translation

    Target ORF information:

    RefSeq Version XM_011240076.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus G protein-coupled receptor 149 (Gpr149), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011240076.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGTCTTTCT TTCTCAGCAA CTTAACAAAT GACTCTAACC TGTGGAAAGC AAGTCATAAT 
    TCTACGGAGA CGGACCTTAT GAATTCACCA GCAACTCTGA CTCTTTCTCT CTTTTGCCTG
    ATCTGTATAA TGACTCTCGC AGCTCTGGTG GGCAGCATTT TCTCACTGGT TTCGCTGCTG
    ACCATGCAAT ACAGAACTGT TTTGTCCATA CTTGTGACAT CTTGGTCTGT GGATGATCTC
    TTGAGCGTCT TGTCCGTGGC CATCTTCATG GTTTTACAGT GGCCAAAAGA GGCACAGGGC
    TACTTCCAGT CTCTGTGCAC CACCTCTGCC CTACTGTATA TGTGCCAGGG CCTCTCCAGC
    AACTTGAAGG CGACTCTCAT AGTCTGCTAC AACTTTTATA CGATGAACAG GACGGTGGAG
    AGTCAGTCAA GCTCCTGGCG ATTAGGACAG GTACTTGGTG TGACTTTAAC CGTTTGGGCA
    GTCAGCCTGC TGCTGGCTTC GCTCCCGCTC TGTGGCTGGG GTGTCTTCGT GCGCACGCCT
    TGGGGCTGCC TGACCGACTG CTCCAGCCCA TACGTGCTGC TCCTCTTCGC GGTATACGCT
    TCCGCTTTCG GGCTCCTGGC GGTTCTCTCT GTCCCTCTCA CTCACCAGCT GCTGTGTTCG
    GAGGAACCGC TGAGACTCCA TGCCAACTAC CAGGAAATTT CTCGTGGCGC CTCTACTCCT
    GGGACCCCTG CTGCTGCGGG AAGAGTGCTC TGTCTGCCGC CAGAGGATGT TGAGATCCCT
    GCTCTGCGAT GCACTGGGGG ATGCTCCCCG AGCTCCGACG TGGTGTTTGC CCCGGGTCAG
    CCGGCTGCCT CCGGCGCTGG AGCCGGCAGG CGAGAGAATC CCGGGACTCC CCAGGGCACC
    AACAGCTTCC CCTTGAGCTT GGCACAGAAG CGCTTTTCTT TGATTCTAGC GCTGACGAAA
    GTCATCCTTT GGCTACCCAT GATGATCCAC ATGGTGGTAA AACACGTGGT GGGGTTTCAG
    AGCCTTCCGG TCGATATGCT CAGCTTTCTA CTAAGCCTGC TGGCCAGCAG TGTAACACCA
    GTGTTTGTCT TATCCAAACG TTGGGCCCAC TTGCCTTGTG GCTGCATCAT CAACTGCCAG
    CCAGACACCT ACTCCGTTGC CTTCGATGGG AAAAAGTCGA AGAGGAAAGG CTTTGAATTC
    AATCTATCAT TCCAACAAAG TTATGGACTC TATAAAATAC CACATGCAGA TTGCTATGAT
    GATGATGAAA ATTCCATATC CTATCACAAC CCAAAGAATT ATGAGTGTGA AGCTACAAAG
    GAGCCTTGGG GAGACAACCG CAGTGTCTTC AATACCATCA CAGTAGAAAT CAGCACCACG
    CCACCGCTGG ACAGCGCCAC ACTGACGGGT GTCCACAAGT GTACCAATAC TGACATCCCA
    GAATCCAAAC AGGCCATGAA AGAGGAAAAG GGTGCTTTTT CTGTTAAAAC AGAAAGCGAT
    ATTAACTATG GAGAAACCAC CTCTTTTGAA GGTCCAGAGA GACGACTGTC CCATGAGGAG
    AATCAGAAAC CAGATCTTTC AGACTGGGAA TGGTGCAGGA GTAAGTCTGA ACGAACGCCT
    CGCCAGGTAC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu06315
    Clone ID Related Accession (Same CDS sequence) NM_177346.5 , NM_177346.4
    Accession Version NM_177346.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2199bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-21
    Organism Mus musculus(house mouse)
    Product probable G-protein coupled receptor 149 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC115721.11 and AC114424.10. On Oct 7, 2017 this sequence version replaced NM_177346.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660815.7771.1, AK136826.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCTTTCT TTCTCAGCAA CTTAACAAAT GACTCTAACC TGTGGAAAGC AAGTCATAAT 
    TCTACGGAGA CGGACCTTAT GAATTCACCA GCAACTCTGA CTCTTTCTCT CTTTTGCCTG
    ATCTGTATAA TGACTCTCGC AGCTCTGGTG GGCAGCATTT TCTCACTGGT TTCGCTGCTG
    ACCATGCAAT ACAGAACTGT TTTGTCCATA CTTGTGACAT CTTGGTCTGT GGATGATCTC
    TTGAGCGTCT TGTCCGTGGC CATCTTCATG GTTTTACAGT GGCCAAAAGA GGCACAGGGC
    TACTTCCAGT CTCTGTGCAC CACCTCTGCC CTACTGTATA TGTGCCAGGG CCTCTCCAGC
    AACTTGAAGG CGACTCTCAT AGTCTGCTAC AACTTTTATA CGATGAACAG GACGGTGGAG
    AGTCAGTCAA GCTCCTGGCG ATTAGGACAG GTACTTGGTG TGACTTTAAC CGTTTGGGCA
    GTCAGCCTGC TGCTGGCTTC GCTCCCGCTC TGTGGCTGGG GTGTCTTCGT GCGCACGCCT
    TGGGGCTGCC TGACCGACTG CTCCAGCCCA TACGTGCTGC TCCTCTTCGC GGTATACGCT
    TCCGCTTTCG GGCTCCTGGC GGTTCTCTCT GTCCCTCTCA CTCACCAGCT GCTGTGTTCG
    GAGGAACCGC TGAGACTCCA TGCCAACTAC CAGGAAATTT CTCGTGGCGC CTCTACTCCT
    GGGACCCCTG CTGCTGCGGG AAGAGTGCTC TGTCTGCCGC CAGAGGATGT TGAGATCCCT
    GCTCTGCGAT GCACTGGGGG ATGCTCCCCG AGCTCCGACG TGGTGTTTGC CCCGGGTCAG
    CCGGCTGCCT CCGGCGCTGG AGCCGGCAGG CGAGAGAATC CCGGGACTCC CCAGGGCACC
    AACAGCTTCC CCTTGAGCTT GGCACAGAAG CGCTTTTCTT TGATTCTAGC GCTGACGAAA
    GTCATCCTTT GGCTACCCAT GATGATCCAC ATGGTGGTAA AACACGTGGT GGGGTTTCAG
    AGCCTTCCGG TCGATATGCT CAGCTTTCTA CTAAGCCTGC TGGCCAGCAG TGTAACACCA
    GTGTTTGTCT TATCCAAACG TTGGGCCCAC TTGCCTTGTG GCTGCATCAT CAACTGCCAG
    CCAGACACCT ACTCCGTTGC CTTCGATGGG AAAAAGTCGA AGAGGAAAGG CTTTGAATTC
    AATCTATCAT TCCAACAAAG TTATGGACTC TATAAAATAC CACATGCAGA TTGCTATGAT
    GATGATGAAA ATTCCATATC CTATCACAAC CCAAAGAATT ATGAGTGTGA AGCTACAAAG
    GAGCCTTGGG GAGACAACCG CAGTGTCTTC AATACCATCA CAGTAGAAAT CAGCACCACG
    CCACCGCTGG ACAGCGCCAC ACTGACGGGT GTCCACAAGT GTACCAATAC TGACATCCCA
    GAATCCAAAC AGGCCATGAA AGAGGAAAAG GGTGCTTTTT CTGTTAAAAC AGAAAGCGAT
    ATTAACTATG GAGAAACCAC CTCTTTTGAA GGTCCAGAGA GACGACTGTC CCATGAGGAG
    AATCAGAAAC CAGATCTTTC AGACTGGGAA TGGTGCAGGA GTAAGTCTGA ACGAACGCCT
    CGCCAGCGTT CCGGTGGTGG GCTGGCTATT CCCATATGCG CGTTCCAGGG GACTGTGTCT
    CTCCAAGCAC CTACTGGGAA AACGCTCTCT CTTTCTACTT ATGAGGTCAG CGCAGAAGGG
    CAAAAGATAA CCCCAGCCTC TAAGAAAATA GAAGTCTATC GATCTAAAAG TGTTGGCCAT
    GAACCGAACT CAGAGGAGTC TCCCTCCACG TTTGCGGACA CCAGTGTAAA AATACACTTG
    GAAGTTCTTG AAATTTGTGA CAACGATGAG GCTCTAGACA CTGTGTCAAT TATCAGCAAC
    ATCAGCCAAT CCTCCACAAA AGTCAGGTCT CCTTCTCTGC GCTACTCACG GAAGGAAAAC
    AGATTCGTCT CCTGCGACTT AGGAGAAACT GCCTCATACT CGCTCTTTTT ACCCACAAGT
    GATCCTGATG GTGATATTAA CATCTCTATC CCTGACACGG TGGAGGCCCA CAGACAGAAC
    AGCAGGCGGC AGCATGAAGA GAGGGATGGC TACCAGGAAG AGATCCAGTT GTTAAACAAA
    GCCTACAGGA AAAGAGAGGC AGAAAGCAAG GGTGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_796320.2
    CDS565..2763
    Translation

    Target ORF information:

    RefSeq Version NM_177346.5
    Organism Mus musculus(house mouse)
    Definition Mus musculus G protein-coupled receptor 149 (Gpr149), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_177346.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCTTTCT TTCTCAGCAA CTTAACAAAT GACTCTAACC TGTGGAAAGC AAGTCATAAT 
    TCTACGGAGA CGGACCTTAT GAATTCACCA GCAACTCTGA CTCTTTCTCT CTTTTGCCTG
    ATCTGTATAA TGACTCTCGC AGCTCTGGTG GGCAGCATTT TCTCACTGGT TTCGCTGCTG
    ACCATGCAAT ACAGAACTGT TTTGTCCATA CTTGTGACAT CTTGGTCTGT GGATGATCTC
    TTGAGCGTCT TGTCCGTGGC CATCTTCATG GTTTTACAGT GGCCAAAAGA GGCACAGGGC
    TACTTCCAGT CTCTGTGCAC CACCTCTGCC CTACTGTATA TGTGCCAGGG CCTCTCCAGC
    AACTTGAAGG CGACTCTCAT AGTCTGCTAC AACTTTTATA CGATGAACAG GACGGTGGAG
    AGTCAGTCAA GCTCCTGGCG ATTAGGACAG GTACTTGGTG TGACTTTAAC CGTTTGGGCA
    GTCAGCCTGC TGCTGGCTTC GCTCCCGCTC TGTGGCTGGG GTGTCTTCGT GCGCACGCCT
    TGGGGCTGCC TGACCGACTG CTCCAGCCCA TACGTGCTGC TCCTCTTCGC GGTATACGCT
    TCCGCTTTCG GGCTCCTGGC GGTTCTCTCT GTCCCTCTCA CTCACCAGCT GCTGTGTTCG
    GAGGAACCGC TGAGACTCCA TGCCAACTAC CAGGAAATTT CTCGTGGCGC CTCTACTCCT
    GGGACCCCTG CTGCTGCGGG AAGAGTGCTC TGTCTGCCGC CAGAGGATGT TGAGATCCCT
    GCTCTGCGAT GCACTGGGGG ATGCTCCCCG AGCTCCGACG TGGTGTTTGC CCCGGGTCAG
    CCGGCTGCCT CCGGCGCTGG AGCCGGCAGG CGAGAGAATC CCGGGACTCC CCAGGGCACC
    AACAGCTTCC CCTTGAGCTT GGCACAGAAG CGCTTTTCTT TGATTCTAGC GCTGACGAAA
    GTCATCCTTT GGCTACCCAT GATGATCCAC ATGGTGGTAA AACACGTGGT GGGGTTTCAG
    AGCCTTCCGG TCGATATGCT CAGCTTTCTA CTAAGCCTGC TGGCCAGCAG TGTAACACCA
    GTGTTTGTCT TATCCAAACG TTGGGCCCAC TTGCCTTGTG GCTGCATCAT CAACTGCCAG
    CCAGACACCT ACTCCGTTGC CTTCGATGGG AAAAAGTCGA AGAGGAAAGG CTTTGAATTC
    AATCTATCAT TCCAACAAAG TTATGGACTC TATAAAATAC CACATGCAGA TTGCTATGAT
    GATGATGAAA ATTCCATATC CTATCACAAC CCAAAGAATT ATGAGTGTGA AGCTACAAAG
    GAGCCTTGGG GAGACAACCG CAGTGTCTTC AATACCATCA CAGTAGAAAT CAGCACCACG
    CCACCGCTGG ACAGCGCCAC ACTGACGGGT GTCCACAAGT GTACCAATAC TGACATCCCA
    GAATCCAAAC AGGCCATGAA AGAGGAAAAG GGTGCTTTTT CTGTTAAAAC AGAAAGCGAT
    ATTAACTATG GAGAAACCAC CTCTTTTGAA GGTCCAGAGA GACGACTGTC CCATGAGGAG
    AATCAGAAAC CAGATCTTTC AGACTGGGAA TGGTGCAGGA GTAAGTCTGA ACGAACGCCT
    CGCCAGCGTT CCGGTGGTGG GCTGGCTATT CCCATATGCG CGTTCCAGGG GACTGTGTCT
    CTCCAAGCAC CTACTGGGAA AACGCTCTCT CTTTCTACTT ATGAGGTCAG CGCAGAAGGG
    CAAAAGATAA CCCCAGCCTC TAAGAAAATA GAAGTCTATC GATCTAAAAG TGTTGGCCAT
    GAACCGAACT CAGAGGAGTC TCCCTCCACG TTTGCGGACA CCAGTGTAAA AATACACTTG
    GAAGTTCTTG AAATTTGTGA CAACGATGAG GCTCTAGACA CTGTGTCAAT TATCAGCAAC
    ATCAGCCAAT CCTCCACAAA AGTCAGGTCT CCTTCTCTGC GCTACTCACG GAAGGAAAAC
    AGATTCGTCT CCTGCGACTT AGGAGAAACT GCCTCATACT CGCTCTTTTT ACCCACAAGT
    GATCCTGATG GTGATATTAA CATCTCTATC CCTGACACGG TGGAGGCCCA CAGACAGAAC
    AGCAGGCGGC AGCATGAAGA GAGGGATGGC TACCAGGAAG AGATCCAGTT GTTAAACAAA
    GCCTACAGGA AAAGAGAGGC AGAAAGCAAG GGTGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu06315
    Clone ID Related Accession (Same CDS sequence) NM_177346.5 , NM_177346.4
    Accession Version NM_177346.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2199bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-12
    Organism Mus musculus(House mouse)
    Product probable G-protein coupled receptor 149
    Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC115721.11 and AC114424.10. On May 2, 2007 this sequence version replaced NM_177346.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK136826.1, AK166029.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCTTTCT TTCTCAGCAA CTTAACAAAT GACTCTAACC TGTGGAAAGC AAGTCATAAT 
    TCTACGGAGA CGGACCTTAT GAATTCACCA GCAACTCTGA CTCTTTCTCT CTTTTGCCTG
    ATCTGTATAA TGACTCTCGC AGCTCTGGTG GGCAGCATTT TCTCACTGGT TTCGCTGCTG
    ACCATGCAAT ACAGAACTGT TTTGTCCATA CTTGTGACAT CTTGGTCTGT GGATGATCTC
    TTGAGCGTCT TGTCCGTGGC CATCTTCATG GTTTTACAGT GGCCAAAAGA GGCACAGGGC
    TACTTCCAGT CTCTGTGCAC CACCTCTGCC CTACTGTATA TGTGCCAGGG CCTCTCCAGC
    AACTTGAAGG CGACTCTCAT AGTCTGCTAC AACTTTTATA CGATGAACAG GACGGTGGAG
    AGTCAGTCAA GCTCCTGGCG ATTAGGACAG GTACTTGGTG TGACTTTAAC CGTTTGGGCA
    GTCAGCCTGC TGCTGGCTTC GCTCCCGCTC TGTGGCTGGG GTGTCTTCGT GCGCACGCCT
    TGGGGCTGCC TGACCGACTG CTCCAGCCCA TACGTGCTGC TCCTCTTCGC GGTATACGCT
    TCCGCTTTCG GGCTCCTGGC GGTTCTCTCT GTCCCTCTCA CTCACCAGCT GCTGTGTTCG
    GAGGAACCGC TGAGACTCCA TGCCAACTAC CAGGAAATTT CTCGTGGCGC CTCTACTCCT
    GGGACCCCTG CTGCTGCGGG AAGAGTGCTC TGTCTGCCGC CAGAGGATGT TGAGATCCCT
    GCTCTGCGAT GCACTGGGGG ATGCTCCCCG AGCTCCGACG TGGTGTTTGC CCCGGGTCAG
    CCGGCTGCCT CCGGCGCTGG AGCCGGCAGG CGAGAGAATC CCGGGACTCC CCAGGGCACC
    AACAGCTTCC CCTTGAGCTT GGCACAGAAG CGCTTTTCTT TGATTCTAGC GCTGACGAAA
    GTCATCCTTT GGCTACCCAT GATGATCCAC ATGGTGGTAA AACACGTGGT GGGGTTTCAG
    AGCCTTCCGG TCGATATGCT CAGCTTTCTA CTAAGCCTGC TGGCCAGCAG TGTAACACCA
    GTGTTTGTCT TATCCAAACG TTGGGCCCAC TTGCCTTGTG GCTGCATCAT CAACTGCCAG
    CCAGACACCT ACTCCGTTGC CTTCGATGGG AAAAAGTCGA AGAGGAAAGG CTTTGAATTC
    AATCTATCAT TCCAACAAAG TTATGGACTC TATAAAATAC CACATGCAGA TTGCTATGAT
    GATGATGAAA ATTCCATATC CTATCACAAC CCAAAGAATT ATGAGTGTGA AGCTACAAAG
    GAGCCTTGGG GAGACAACCG CAGTGTCTTC AATACCATCA CAGTAGAAAT CAGCACCACG
    CCACCGCTGG ACAGCGCCAC ACTGACGGGT GTCCACAAGT GTACCAATAC TGACATCCCA
    GAATCCAAAC AGGCCATGAA AGAGGAAAAG GGTGCTTTTT CTGTTAAAAC AGAAAGCGAT
    ATTAACTATG GAGAAACCAC CTCTTTTGAA GGTCCAGAGA GACGACTGTC CCATGAGGAG
    AATCAGAAAC CAGATCTTTC AGACTGGGAA TGGTGCAGGA GTAAGTCTGA ACGAACGCCT
    CGCCAGCGTT CCGGTGGTGG GCTGGCTATT CCCATATGCG CGTTCCAGGG GACTGTGTCT
    CTCCAAGCAC CTACTGGGAA AACGCTCTCT CTTTCTACTT ATGAGGTCAG CGCAGAAGGG
    CAAAAGATAA CCCCAGCCTC TAAGAAAATA GAAGTCTATC GATCTAAAAG TGTTGGCCAT
    GAACCGAACT CAGAGGAGTC TCCCTCCACG TTTGCGGACA CCAGTGTAAA AATACACTTG
    GAAGTTCTTG AAATTTGTGA CAACGATGAG GCTCTAGACA CTGTGTCAAT TATCAGCAAC
    ATCAGCCAAT CCTCCACAAA AGTCAGGTCT CCTTCTCTGC GCTACTCACG GAAGGAAAAC
    AGATTCGTCT CCTGCGACTT AGGAGAAACT GCCTCATACT CGCTCTTTTT ACCCACAAGT
    GATCCTGATG GTGATATTAA CATCTCTATC CCTGACACGG TGGAGGCCCA CAGACAGAAC
    AGCAGGCGGC AGCATGAAGA GAGGGATGGC TACCAGGAAG AGATCCAGTT GTTAAACAAA
    GCCTACAGGA AAAGAGAGGC AGAAAGCAAG GGTGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_796320.2
    CDS565..2763
    Translation

    Target ORF information:

    RefSeq Version NM_177346.4
    Organism Mus musculus(House mouse)
    Definition Mus musculus G protein-coupled receptor 149 (Gpr149), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_177346.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCTTTCT TTCTCAGCAA CTTAACAAAT GACTCTAACC TGTGGAAAGC AAGTCATAAT 
    TCTACGGAGA CGGACCTTAT GAATTCACCA GCAACTCTGA CTCTTTCTCT CTTTTGCCTG
    ATCTGTATAA TGACTCTCGC AGCTCTGGTG GGCAGCATTT TCTCACTGGT TTCGCTGCTG
    ACCATGCAAT ACAGAACTGT TTTGTCCATA CTTGTGACAT CTTGGTCTGT GGATGATCTC
    TTGAGCGTCT TGTCCGTGGC CATCTTCATG GTTTTACAGT GGCCAAAAGA GGCACAGGGC
    TACTTCCAGT CTCTGTGCAC CACCTCTGCC CTACTGTATA TGTGCCAGGG CCTCTCCAGC
    AACTTGAAGG CGACTCTCAT AGTCTGCTAC AACTTTTATA CGATGAACAG GACGGTGGAG
    AGTCAGTCAA GCTCCTGGCG ATTAGGACAG GTACTTGGTG TGACTTTAAC CGTTTGGGCA
    GTCAGCCTGC TGCTGGCTTC GCTCCCGCTC TGTGGCTGGG GTGTCTTCGT GCGCACGCCT
    TGGGGCTGCC TGACCGACTG CTCCAGCCCA TACGTGCTGC TCCTCTTCGC GGTATACGCT
    TCCGCTTTCG GGCTCCTGGC GGTTCTCTCT GTCCCTCTCA CTCACCAGCT GCTGTGTTCG
    GAGGAACCGC TGAGACTCCA TGCCAACTAC CAGGAAATTT CTCGTGGCGC CTCTACTCCT
    GGGACCCCTG CTGCTGCGGG AAGAGTGCTC TGTCTGCCGC CAGAGGATGT TGAGATCCCT
    GCTCTGCGAT GCACTGGGGG ATGCTCCCCG AGCTCCGACG TGGTGTTTGC CCCGGGTCAG
    CCGGCTGCCT CCGGCGCTGG AGCCGGCAGG CGAGAGAATC CCGGGACTCC CCAGGGCACC
    AACAGCTTCC CCTTGAGCTT GGCACAGAAG CGCTTTTCTT TGATTCTAGC GCTGACGAAA
    GTCATCCTTT GGCTACCCAT GATGATCCAC ATGGTGGTAA AACACGTGGT GGGGTTTCAG
    AGCCTTCCGG TCGATATGCT CAGCTTTCTA CTAAGCCTGC TGGCCAGCAG TGTAACACCA
    GTGTTTGTCT TATCCAAACG TTGGGCCCAC TTGCCTTGTG GCTGCATCAT CAACTGCCAG
    CCAGACACCT ACTCCGTTGC CTTCGATGGG AAAAAGTCGA AGAGGAAAGG CTTTGAATTC
    AATCTATCAT TCCAACAAAG TTATGGACTC TATAAAATAC CACATGCAGA TTGCTATGAT
    GATGATGAAA ATTCCATATC CTATCACAAC CCAAAGAATT ATGAGTGTGA AGCTACAAAG
    GAGCCTTGGG GAGACAACCG CAGTGTCTTC AATACCATCA CAGTAGAAAT CAGCACCACG
    CCACCGCTGG ACAGCGCCAC ACTGACGGGT GTCCACAAGT GTACCAATAC TGACATCCCA
    GAATCCAAAC AGGCCATGAA AGAGGAAAAG GGTGCTTTTT CTGTTAAAAC AGAAAGCGAT
    ATTAACTATG GAGAAACCAC CTCTTTTGAA GGTCCAGAGA GACGACTGTC CCATGAGGAG
    AATCAGAAAC CAGATCTTTC AGACTGGGAA TGGTGCAGGA GTAAGTCTGA ACGAACGCCT
    CGCCAGCGTT CCGGTGGTGG GCTGGCTATT CCCATATGCG CGTTCCAGGG GACTGTGTCT
    CTCCAAGCAC CTACTGGGAA AACGCTCTCT CTTTCTACTT ATGAGGTCAG CGCAGAAGGG
    CAAAAGATAA CCCCAGCCTC TAAGAAAATA GAAGTCTATC GATCTAAAAG TGTTGGCCAT
    GAACCGAACT CAGAGGAGTC TCCCTCCACG TTTGCGGACA CCAGTGTAAA AATACACTTG
    GAAGTTCTTG AAATTTGTGA CAACGATGAG GCTCTAGACA CTGTGTCAAT TATCAGCAAC
    ATCAGCCAAT CCTCCACAAA AGTCAGGTCT CCTTCTCTGC GCTACTCACG GAAGGAAAAC
    AGATTCGTCT CCTGCGACTT AGGAGAAACT GCCTCATACT CGCTCTTTTT ACCCACAAGT
    GATCCTGATG GTGATATTAA CATCTCTATC CCTGACACGG TGGAGGCCCA CAGACAGAAC
    AGCAGGCGGC AGCATGAAGA GAGGGATGGC TACCAGGAAG AGATCCAGTT GTTAAACAAA
    GCCTACAGGA AAAGAGAGGC AGAAAGCAAG GGTGATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu73457
    Clone ID Related Accession (Same CDS sequence) XM_011240076.1 , XM_011240076.2
    Accession Version XM_011240076.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1635bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product probable G-protein coupled receptor 149 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011240076.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGTCTTTCT TTCTCAGCAA CTTAACAAAT GACTCTAACC TGTGGAAAGC AAGTCATAAT 
    TCTACGGAGA CGGACCTTAT GAATTCACCA GCAACTCTGA CTCTTTCTCT CTTTTGCCTG
    ATCTGTATAA TGACTCTCGC AGCTCTGGTG GGCAGCATTT TCTCACTGGT TTCGCTGCTG
    ACCATGCAAT ACAGAACTGT TTTGTCCATA CTTGTGACAT CTTGGTCTGT GGATGATCTC
    TTGAGCGTCT TGTCCGTGGC CATCTTCATG GTTTTACAGT GGCCAAAAGA GGCACAGGGC
    TACTTCCAGT CTCTGTGCAC CACCTCTGCC CTACTGTATA TGTGCCAGGG CCTCTCCAGC
    AACTTGAAGG CGACTCTCAT AGTCTGCTAC AACTTTTATA CGATGAACAG GACGGTGGAG
    AGTCAGTCAA GCTCCTGGCG ATTAGGACAG GTACTTGGTG TGACTTTAAC CGTTTGGGCA
    GTCAGCCTGC TGCTGGCTTC GCTCCCGCTC TGTGGCTGGG GTGTCTTCGT GCGCACGCCT
    TGGGGCTGCC TGACCGACTG CTCCAGCCCA TACGTGCTGC TCCTCTTCGC GGTATACGCT
    TCCGCTTTCG GGCTCCTGGC GGTTCTCTCT GTCCCTCTCA CTCACCAGCT GCTGTGTTCG
    GAGGAACCGC TGAGACTCCA TGCCAACTAC CAGGAAATTT CTCGTGGCGC CTCTACTCCT
    GGGACCCCTG CTGCTGCGGG AAGAGTGCTC TGTCTGCCGC CAGAGGATGT TGAGATCCCT
    GCTCTGCGAT GCACTGGGGG ATGCTCCCCG AGCTCCGACG TGGTGTTTGC CCCGGGTCAG
    CCGGCTGCCT CCGGCGCTGG AGCCGGCAGG CGAGAGAATC CCGGGACTCC CCAGGGCACC
    AACAGCTTCC CCTTGAGCTT GGCACAGAAG CGCTTTTCTT TGATTCTAGC GCTGACGAAA
    GTCATCCTTT GGCTACCCAT GATGATCCAC ATGGTGGTAA AACACGTGGT GGGGTTTCAG
    AGCCTTCCGG TCGATATGCT CAGCTTTCTA CTAAGCCTGC TGGCCAGCAG TGTAACACCA
    GTGTTTGTCT TATCCAAACG TTGGGCCCAC TTGCCTTGTG GCTGCATCAT CAACTGCCAG
    CCAGACACCT ACTCCGTTGC CTTCGATGGG AAAAAGTCGA AGAGGAAAGG CTTTGAATTC
    AATCTATCAT TCCAACAAAG TTATGGACTC TATAAAATAC CACATGCAGA TTGCTATGAT
    GATGATGAAA ATTCCATATC CTATCACAAC CCAAAGAATT ATGAGTGTGA AGCTACAAAG
    GAGCCTTGGG GAGACAACCG CAGTGTCTTC AATACCATCA CAGTAGAAAT CAGCACCACG
    CCACCGCTGG ACAGCGCCAC ACTGACGGGT GTCCACAAGT GTACCAATAC TGACATCCCA
    GAATCCAAAC AGGCCATGAA AGAGGAAAAG GGTGCTTTTT CTGTTAAAAC AGAAAGCGAT
    ATTAACTATG GAGAAACCAC CTCTTTTGAA GGTCCAGAGA GACGACTGTC CCATGAGGAG
    AATCAGAAAC CAGATCTTTC AGACTGGGAA TGGTGCAGGA GTAAGTCTGA ACGAACGCCT
    CGCCAGGTAC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011238378.1
    CDS626..2260
    Translation

    Target ORF information:

    RefSeq Version XM_011240076.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus G protein-coupled receptor 149 (Gpr149), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011240076.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGTCTTTCT TTCTCAGCAA CTTAACAAAT GACTCTAACC TGTGGAAAGC AAGTCATAAT 
    TCTACGGAGA CGGACCTTAT GAATTCACCA GCAACTCTGA CTCTTTCTCT CTTTTGCCTG
    ATCTGTATAA TGACTCTCGC AGCTCTGGTG GGCAGCATTT TCTCACTGGT TTCGCTGCTG
    ACCATGCAAT ACAGAACTGT TTTGTCCATA CTTGTGACAT CTTGGTCTGT GGATGATCTC
    TTGAGCGTCT TGTCCGTGGC CATCTTCATG GTTTTACAGT GGCCAAAAGA GGCACAGGGC
    TACTTCCAGT CTCTGTGCAC CACCTCTGCC CTACTGTATA TGTGCCAGGG CCTCTCCAGC
    AACTTGAAGG CGACTCTCAT AGTCTGCTAC AACTTTTATA CGATGAACAG GACGGTGGAG
    AGTCAGTCAA GCTCCTGGCG ATTAGGACAG GTACTTGGTG TGACTTTAAC CGTTTGGGCA
    GTCAGCCTGC TGCTGGCTTC GCTCCCGCTC TGTGGCTGGG GTGTCTTCGT GCGCACGCCT
    TGGGGCTGCC TGACCGACTG CTCCAGCCCA TACGTGCTGC TCCTCTTCGC GGTATACGCT
    TCCGCTTTCG GGCTCCTGGC GGTTCTCTCT GTCCCTCTCA CTCACCAGCT GCTGTGTTCG
    GAGGAACCGC TGAGACTCCA TGCCAACTAC CAGGAAATTT CTCGTGGCGC CTCTACTCCT
    GGGACCCCTG CTGCTGCGGG AAGAGTGCTC TGTCTGCCGC CAGAGGATGT TGAGATCCCT
    GCTCTGCGAT GCACTGGGGG ATGCTCCCCG AGCTCCGACG TGGTGTTTGC CCCGGGTCAG
    CCGGCTGCCT CCGGCGCTGG AGCCGGCAGG CGAGAGAATC CCGGGACTCC CCAGGGCACC
    AACAGCTTCC CCTTGAGCTT GGCACAGAAG CGCTTTTCTT TGATTCTAGC GCTGACGAAA
    GTCATCCTTT GGCTACCCAT GATGATCCAC ATGGTGGTAA AACACGTGGT GGGGTTTCAG
    AGCCTTCCGG TCGATATGCT CAGCTTTCTA CTAAGCCTGC TGGCCAGCAG TGTAACACCA
    GTGTTTGTCT TATCCAAACG TTGGGCCCAC TTGCCTTGTG GCTGCATCAT CAACTGCCAG
    CCAGACACCT ACTCCGTTGC CTTCGATGGG AAAAAGTCGA AGAGGAAAGG CTTTGAATTC
    AATCTATCAT TCCAACAAAG TTATGGACTC TATAAAATAC CACATGCAGA TTGCTATGAT
    GATGATGAAA ATTCCATATC CTATCACAAC CCAAAGAATT ATGAGTGTGA AGCTACAAAG
    GAGCCTTGGG GAGACAACCG CAGTGTCTTC AATACCATCA CAGTAGAAAT CAGCACCACG
    CCACCGCTGG ACAGCGCCAC ACTGACGGGT GTCCACAAGT GTACCAATAC TGACATCCCA
    GAATCCAAAC AGGCCATGAA AGAGGAAAAG GGTGCTTTTT CTGTTAAAAC AGAAAGCGAT
    ATTAACTATG GAGAAACCAC CTCTTTTGAA GGTCCAGAGA GACGACTGTC CCATGAGGAG
    AATCAGAAAC CAGATCTTTC AGACTGGGAA TGGTGCAGGA GTAAGTCTGA ACGAACGCCT
    CGCCAGGTAC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.