Spg20 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Spg20
Entrez Gene ID	229285
Full Name	spastic paraplegia 20, spartin (Troyer syndrome) homolog (human)
Synonyms	AI840044,C79168,SPARTIN,Spart,TAHCCP1,mKIAA0610
General protein information	Preferred Names spastic paraplegia 20, spartin (Troyer syndrome) homolog (human) Names spartin
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3\|3 C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001144987.1	NP_001138459.1	spartin isoform a
XM_006501320.2	XP_006501383.1	spartin isoform X1
XM_030252562.1	XP_030108422.1	spartin isoform X2
NM_144895.2	NP_659144.1	spartin isoform a
NM_001144988.1	NP_001138460.1	spartin isoform b
XM_006501320.3	XP_006501383.1	spartin isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Methylation changes of SIRT1, KLF4, DAPK1 and SPG20 in B-lymphocytes derived from follicular and diffuse large B-cell lymphoma.
Frazzi R, Zanetti E, Pistoni M, Tamagnini I, Valli R, Braglia L, Merli F
Leukemia research5789-96(2017 Jun)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Three cases of Troyer syndrome in two families of Filipino descent.
Butler S, Helbig KL, Alcaraz W, Seaver LH, Hsieh DT, Rohena L
American journal of medical genetics. Part A170(7)1780-5(2016 Jul)

The role of spartin and its novel ubiquitin binding region in DALIS occurrence.
Karlsson AB, Washington J, Dimitrova V, Hooper C, Shekhtman A, Bakowska JC
Molecular biology of the cell25(8)1355-65(2014 Apr)

Are alsin and spartin novel interaction partners?
?obano?lu G, Ozansoy M, Ba?ak AN
Biochemical and biophysical research communications427(1)1-4(2012 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

findings evidence a positive correlation between SIRT1 and BCL6 expression increase in follicular lymphomas (FL) . SIRT1 methylation decreases in FL and diffuse large-B cell lymphomas (DLBCL)and this parallels the increase of KLF4, DAPK1 and SPG20 methylation
Title: Methylation changes of SIRT1, KLF4, DAPK1 and SPG20 in B-lymphocytes derived from follicular and diffuse large B-cell lymphoma.

Here we report the case of three patients of Filipino descent with Troyer syndrome. Whole exome sequencing (WES) identified a homozygous mutation c.364_365delAT which predicts p.Met122Valfs*2 in SPG20
Title: Three cases of Troyer syndrome in two families of Filipino descent.

spartin, via the ubiquitin-binding region, binds Lys-63-linked ubiquitin chains but does not bind Lys-48-linked ubiquitin chains. spartin's role in DALIS formation depends on key residues within its ubiquitin-binding region.
Title: The role of spartin and its novel ubiquitin binding region in DALIS occurrence.

alsin and spartin may interact each other physically
Title: Are alsin and spartin novel interaction partners?

a role for spartin in lipid droplets maintenance and in the regulation of cytokinesis
Title: Spg20-/- mice reveal multimodal functions for Troyer syndrome protein spartin in lipid droplet maintenance, cytokinesis and BMP signaling.

The following Spg20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spg20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu06769	NM_001144988.1 Latest version!	Mus musculus spastic paraplegia 20, spartin (Troyer syndrome) homolog (human) (Spg20), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$363.30 $519.00
OMu06767	NM_144895.2 Latest version!	Mus musculus spastic paraplegia 20, spartin (Troyer syndrome) homolog (human) (Spg20), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu06767	NM_001144987.1 Latest version!	Mus musculus spastic paraplegia 20, spartin (Troyer syndrome) homolog (human) (Spg20), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu06767	XM_006501320.3 Latest version!	Mus musculus spastic paraplegia 20, spartin (Troyer syndrome) homolog (human) (Spg20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu06769	XM_030252562.1 Latest version!	Mus musculus spastic paraplegia 20, spartin (Troyer syndrome) homolog (human) (Spg20), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu06767	XM_006501320.2	Mus musculus spastic paraplegia 20, spartin (Troyer syndrome) homolog (human) (Spg20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Spg20 ( NM_001144988.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001138460.1 Mus musculus spastic paraplegia 20, spartin (Troyer syndrome) homolog (human) (Spg20), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu06769
Clone ID Related Accession (Same CDS sequence)	XM_030252562.1 , NM_001144988.1
Accession Version	NM_001144988.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Mus musculus(house mouse)
Product	spartin isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK159951.1, AK129171.1, AC111132.7 and BU755937.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon in the central coding region, compared to variant 1. The resulting isoform (b) lacks an internal segment, compared to isoform a. ##Evidence-Data-START## Transcript exon combination :: AK129171.1, SRR1660811.190078.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGAGAGAG AACCAGAAAA TGGAGAACCT GCTGAAATTA AGATCATCAA GGAAGCATAC 
GAGAAGGCCT TTATGTTTGT TAATAAAGGA CTGAACACGG ACGAGTTAGG GCAGAAGGAA 
GAAGCAAAGA ACTATTACAA GCAAGGAATA GGACACCTGC TTAGGGGCAT CAGCATCGCG 
GCTGCAGAGC CGGGGCACAC AGGCCCTGCG TGGGAGGCTG CCAGGCAGAT GCAACAGAAA 
ATGAAGGAAA CGCTTCAGAA CGTCCGCACC AGGCTAGAGA TCCTGGAGAA GGGCCTTGCC 
ACTTCTCTAC GGAATGACCT TCAGGATGTG CCCAAGCTAT ACCCAGAGTT CCCGCCTAAA 
GACGCGTGCA AGAAGTCCCC AGAGCAGGAG TCCGTTAGTA CAGCGCCCCA GCGTGCTGAA 
GTCGATGGGA GCGCCTCAGC CGCGTGTGCC GGCCCAAGTG GTGCGCCTTC TGCTCTGCCC 
GTACCATCGC CCAGCTGCCC TGCCGAAGCG CCACCAGCCT ACAGCCCACA GGCTGCTGAG 
GGCCACTACA CTGTTTCCTA TGGAACAGAT TCCGGGGAGT TTTCATCCGT GGGAGAAGAC 
TTCTACAGGA ACCGTTCCCA GCCGCCCCCT CTTGAGACCT TGGGGCTCGA TGCAGATGAG 
TTGATCTTGA TACCAAACGG AGTGCAGATC TTTTTTGTAA ACCCTGCGGG AGAGGTCAGT 
GCTCCCTCGT ACCCTGGGTA TCTGCGGATC GTGCGGTTCT TGGATAATTC TCTGGATACA 
GTTCTGAACC GCCCTCCTGG GTTTCTCCAG GTGTGTGACT GGTTATACCC TCTAGTTCCT 
GATAGGTCAC CAGTTCTGAA GTGCACTGTG GGAGCCTACA TGTTTCCAGA TACCATGTTA 
CAAGCTGCTG GGTGCTTCGT AGGGGTTGTT CTTTCATCTG AGCTCCCAGA AGATGACAGA 
GAGCTGTTTG AGGATCTGTT GAGACAGATG TCTGACCTTC GGCTCCAGAC CAAAGATTCA 
AGTGAAGAAG TTAATCTGAG CCAGATTGTG CCCTGTGAGC CGAGTTCAGA AGAAAAATCG 
AAAGAATTGC CTGAATGGAG CGAGAAAGTG GCCCACAACA TCCTGTCAGG TGCTTCCTGG 
GTGAGCTGGG GTTTAGTCAA AGGTGCTGAA TTTACTGGCA AAGCAATTCA GAAAGGTGCT 
TCTAAACTCA GAGAGCGCAT TCAGCCGGAG GAAAAGCCAG TGGAGGTGAG CCCAGCTGTC 
ACCAGGGGTC TCTACATAGC GAAGCAGGCA ACCGGAGGAG CAGCCAAAGT CAGCCAGCTC 
CTGGTTGACG GCGTCTGCAC AGTAGCGAAT TGTGTTGGTA AAGAACTTGC TCCACACGTC 
AAGAAGCACG GAAGCAAACT GGTTCCGGAA TCCCTGAAGA GGGACAAAGA CGGGAAATCT 
GCCCTGGATG GCGCCATGGT CGTGGCAGCG AGTAGTGTTC AAGGATTCTC AACTGTCTGG 
CAAGGGTTGG AATGTGCAGC TAAATGCATC GTTAACAACG TGTCTGCAGA AACTGTACAG 
ACTGTGAGAT ACAAGTATGG GCACAACGCA GGAGAAGCTA CACATAACGC AGTGGATTCA 
GCTATCAATG TTGGGCTCAC TGCCTACAAT ATTGACAACA TCGGCATCAA AGCCATGGTG 
AAGAAAACTG CCAAGCAGAC GGGACACACT CTCCTCGAGG ACTACCAGAT CGTTGAGCGC 
CCTCAGAGGG AGAGTCAGGG AGGAGCCACA AGCACAGAAG GGAGAAGAGA CATAGGAAAG 
CAGGTGGAGG AAGAGAAGCC AGGGGCAGGA AAGAAGGACA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001138460.1
CDS	113..1957
Translation	MEREPENGEPAEIKIIKEAYEKAFMFVNKGLNTDELGQKEEAKNYYKQGIGHLLRGISIAAAEPGHTGPAWEAARQMQQKMKETLQNVRTRLEILEKGLATSLRNDLQDVPKLYPEFPPKDACKKSPEQESVSTAPQRAEVDGSASAACAGPSGAPSALPVPSPSCPAEAPPAYSPQAAEGHYTVSYGTDSGEFSSVGEDFYRNRSQPPPLETLGLDADELILIPNGVQIFFVNPAGEVSAPSYPGYLRIVRFLDNSLDTVLNRPPGFLQVCDWLYPLVPDRSPVLKCTVGAYMFPDTMLQAAGCFVGVVLSSELPEDDRELFEDLLRQMSDLRLQTKDSSEEVNLSQIVPCEPSSEEKSKELPEWSEKVAHNILSGASWVSWGLVKGAEFTGKAIQKGASKLRERIQPEEKPVEVSPAVTRGLYIAKQATGGAAKVSQLLVDGVCTVANCVGKELAPHVKKHGSKLVPESLKRDKDGKSALDGAMVVAASSVQGFSTVWQGLECAAKCIVNNVSAETVQTVRYKYGHNAGEATHNAVDSAINVGLTAYNIDNIGIKAMVKKTAKQTGHTLLEDYQIVERPQRESQGGATSTEGRRDIGKQVEEEKPGAGKKDK

Target ORF information:

RefSeq Version	NM_001144988.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus spastic paraplegia 20, spartin (Troyer syndrome) homolog (human) (Spg20), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144988.1

ORF Insert Sequence:

ATGGAGAGAG AACCAGAAAA TGGAGAACCT GCTGAAATTA AGATCATCAA GGAAGCATAC 
GAGAAGGCCT TTATGTTTGT TAATAAAGGA CTGAACACGG ACGAGTTAGG GCAGAAGGAA 
GAAGCAAAGA ACTATTACAA GCAAGGAATA GGACACCTGC TTAGGGGCAT CAGCATCGCG 
GCTGCAGAGC CGGGGCACAC AGGCCCTGCG TGGGAGGCTG CCAGGCAGAT GCAACAGAAA 
ATGAAGGAAA CGCTTCAGAA CGTCCGCACC AGGCTAGAGA TCCTGGAGAA GGGCCTTGCC 
ACTTCTCTAC GGAATGACCT TCAGGATGTG CCCAAGCTAT ACCCAGAGTT CCCGCCTAAA 
GACGCGTGCA AGAAGTCCCC AGAGCAGGAG TCCGTTAGTA CAGCGCCCCA GCGTGCTGAA 
GTCGATGGGA GCGCCTCAGC CGCGTGTGCC GGCCCAAGTG GTGCGCCTTC TGCTCTGCCC 
GTACCATCGC CCAGCTGCCC TGCCGAAGCG CCACCAGCCT ACAGCCCACA GGCTGCTGAG 
GGCCACTACA CTGTTTCCTA TGGAACAGAT TCCGGGGAGT TTTCATCCGT GGGAGAAGAC 
TTCTACAGGA ACCGTTCCCA GCCGCCCCCT CTTGAGACCT TGGGGCTCGA TGCAGATGAG 
TTGATCTTGA TACCAAACGG AGTGCAGATC TTTTTTGTAA ACCCTGCGGG AGAGGTCAGT 
GCTCCCTCGT ACCCTGGGTA TCTGCGGATC GTGCGGTTCT TGGATAATTC TCTGGATACA 
GTTCTGAACC GCCCTCCTGG GTTTCTCCAG GTGTGTGACT GGTTATACCC TCTAGTTCCT 
GATAGGTCAC CAGTTCTGAA GTGCACTGTG GGAGCCTACA TGTTTCCAGA TACCATGTTA 
CAAGCTGCTG GGTGCTTCGT AGGGGTTGTT CTTTCATCTG AGCTCCCAGA AGATGACAGA 
GAGCTGTTTG AGGATCTGTT GAGACAGATG TCTGACCTTC GGCTCCAGAC CAAAGATTCA 
AGTGAAGAAG TTAATCTGAG CCAGATTGTG CCCTGTGAGC CGAGTTCAGA AGAAAAATCG 
AAAGAATTGC CTGAATGGAG CGAGAAAGTG GCCCACAACA TCCTGTCAGG TGCTTCCTGG 
GTGAGCTGGG GTTTAGTCAA AGGTGCTGAA TTTACTGGCA AAGCAATTCA GAAAGGTGCT 
TCTAAACTCA GAGAGCGCAT TCAGCCGGAG GAAAAGCCAG TGGAGGTGAG CCCAGCTGTC 
ACCAGGGGTC TCTACATAGC GAAGCAGGCA ACCGGAGGAG CAGCCAAAGT CAGCCAGCTC 
CTGGTTGACG GCGTCTGCAC AGTAGCGAAT TGTGTTGGTA AAGAACTTGC TCCACACGTC 
AAGAAGCACG GAAGCAAACT GGTTCCGGAA TCCCTGAAGA GGGACAAAGA CGGGAAATCT 
GCCCTGGATG GCGCCATGGT CGTGGCAGCG AGTAGTGTTC AAGGATTCTC AACTGTCTGG 
CAAGGGTTGG AATGTGCAGC TAAATGCATC GTTAACAACG TGTCTGCAGA AACTGTACAG 
ACTGTGAGAT ACAAGTATGG GCACAACGCA GGAGAAGCTA CACATAACGC AGTGGATTCA 
GCTATCAATG TTGGGCTCAC TGCCTACAAT ATTGACAACA TCGGCATCAA AGCCATGGTG 
AAGAAAACTG CCAAGCAGAC GGGACACACT CTCCTCGAGG ACTACCAGAT CGTTGAGCGC 
CCTCAGAGGG AGAGTCAGGG AGGAGCCACA AGCACAGAAG GGAGAAGAGA CATAGGAAAG 
CAGGTGGAGG AAGAGAAGCC AGGGGCAGGA AAGAAGGACA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spg20 ( NM_001144987.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001138459.1 Mus musculus spastic paraplegia 20, spartin (Troyer syndrome) homolog (human) (Spg20), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu06767
Clone ID Related Accession (Same CDS sequence)	NM_001144987.1 , XM_006501320.2 , NM_144895.2 , XM_006501320.3
Accession Version	NM_001144987.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Mus musculus(house mouse)
Product	spartin isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK147760.1, AC127334.3, AK159951.1, AK129171.1 and BU755937.1. Transcript Variant: This variant (2) uses an alternate 5' UTR, compared to variant 1. Both variants 1 and 2 encode the same isoform (a). ##Evidence-Data-START## Transcript exon combination :: AK147760.1, SRR1660815.223937.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849381 [ECO:0000348] ##Evidence-Data-END##

ATGGAGAGAG AACCAGAAAA TGGAGAACCT GCTGAAATTA AGATCATCAA GGAAGCATAC 
GAGAAGGCCT TTATGTTTGT TAATAAAGGA CTGAACACGG ACGAGTTAGG GCAGAAGGAA 
GAAGCAAAGA ACTATTACAA GCAAGGAATA GGACACCTGC TTAGGGGCAT CAGCATCGCG 
GCTGCAGAGC CGGGGCACAC AGGCCCTGCG TGGGAGGCTG CCAGGCAGAT GCAACAGAAA 
ATGAAGGAAA CGCTTCAGAA CGTCCGCACC AGGCTAGAGA TCCTGGAGAA GGGCCTTGCC 
ACTTCTCTAC GGAATGACCT TCAGGATGTG CCCAAGCTAT ACCCAGAGTT CCCGCCTAAA 
GACGCGTGCA AGAAGTCCCC AGAGCAGGAG TCCGTTAGTA CAGCGCCCCA GCGTGCTGAA 
GTCGATGGGA GCGCCTCAGC CGCGTGTGCC GGCCCAAGTG GTGCGCCTTC TGCTCTGCCC 
GTACCATCGC CCAGCTGCCC TGCCGAAGCG CCACCAGCCT ACAGCCCACA GGCTGCTGAG 
GGCCACTACA CTGTTTCCTA TGGAACAGAT TCCGGGGAGT TTTCATCCGT GGGAGAAGAC 
TTCTACAGGA ACCGTTCCCA GCCGCCCCCT CTTGAGACCT TGGGGCTCGA TGCAGATGAG 
TTGATCTTGA TACCAAACGG AGTGCAGATC TTTTTTGTAA ACCCTGCGGG AGAGGTCAGT 
GCTCCCTCGT ACCCTGGGTA TCTGCGGATC GTGCGGTTCT TGGATAATTC TCTGGATACA 
GTTCTGAACC GCCCTCCTGG GTTTCTCCAG GTGTGTGACT GGTTATACCC TCTAGTTCCT 
GATAGGTCAC CAGTTCTGAA GTGCACTGTG GGAGCCTACA TGTTTCCAGA TACCATGTTA 
CAAGCTGCTG GGTGCTTCGT AGGGGTTGTT CTTTCATCTG AGCTCCCAGA AGATGACAGA 
GAGCTGTTTG AGGATCTGTT GAGACAGATG TCTGACCTTC GGCTCCAGGC CAACTGGAAT 
AGAGAAGAAG ATGAATTCCA AATCCCTGGG AGATCAAGTC ACCCCTCTGA ACCACCGAAG 
GAAGCTTCTG GCACTGATGT CAGGCAGTCA AGTTCTTCAG GTTCCTCAAT AGACCAAGGC 
AGCAAGGATG CCCGCCATAA AGGAAAGCGT GGGAAAAAGA CCAAAGATTC AAGTGAAGAA 
GTTAATCTGA GCCAGATTGT GCCCTGTGAG CCGAGTTCAG AAGAAAAATC GAAAGAATTG 
CCTGAATGGA GCGAGAAAGT GGCCCACAAC ATCCTGTCAG GTGCTTCCTG GGTGAGCTGG 
GGTTTAGTCA AAGGTGCTGA ATTTACTGGC AAAGCAATTC AGAAAGGTGC TTCTAAACTC 
AGAGAGCGCA TTCAGCCGGA GGAAAAGCCA GTGGAGGTGA GCCCAGCTGT CACCAGGGGT 
CTCTACATAG CGAAGCAGGC AACCGGAGGA GCAGCCAAAG TCAGCCAGCT CCTGGTTGAC 
GGCGTCTGCA CAGTAGCGAA TTGTGTTGGT AAAGAACTTG CTCCACACGT CAAGAAGCAC 
GGAAGCAAAC TGGTTCCGGA ATCCCTGAAG AGGGACAAAG ACGGGAAATC TGCCCTGGAT 
GGCGCCATGG TCGTGGCAGC GAGTAGTGTT CAAGGATTCT CAACTGTCTG GCAAGGGTTG 
GAATGTGCAG CTAAATGCAT CGTTAACAAC GTGTCTGCAG AAACTGTACA GACTGTGAGA 
TACAAGTATG GGCACAACGC AGGAGAAGCT ACACATAACG CAGTGGATTC AGCTATCAAT 
GTTGGGCTCA CTGCCTACAA TATTGACAAC ATCGGCATCA AAGCCATGGT GAAGAAAACT 
GCCAAGCAGA CGGGACACAC TCTCCTCGAG GACTACCAGA TCGTTGAGCG CCCTCAGAGG 
GAGAGTCAGG GAGGAGCCAC AAGCACAGAA GGGAGAAGAG ACATAGGAAA GCAGGTGGAG 
GAAGAGAAGC CAGGGGCAGG AAAGAAGGAC AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001138459.1
CDS	329..2344
Translation	MEREPENGEPAEIKIIKEAYEKAFMFVNKGLNTDELGQKEEAKNYYKQGIGHLLRGISIAAAEPGHTGPAWEAARQMQQKMKETLQNVRTRLEILEKGLATSLRNDLQDVPKLYPEFPPKDACKKSPEQESVSTAPQRAEVDGSASAACAGPSGAPSALPVPSPSCPAEAPPAYSPQAAEGHYTVSYGTDSGEFSSVGEDFYRNRSQPPPLETLGLDADELILIPNGVQIFFVNPAGEVSAPSYPGYLRIVRFLDNSLDTVLNRPPGFLQVCDWLYPLVPDRSPVLKCTVGAYMFPDTMLQAAGCFVGVVLSSELPEDDRELFEDLLRQMSDLRLQANWNREEDEFQIPGRSSHPSEPPKEASGTDVRQSSSSGSSIDQGSKDARHKGKRGKKTKDSSEEVNLSQIVPCEPSSEEKSKELPEWSEKVAHNILSGASWVSWGLVKGAEFTGKAIQKGASKLRERIQPEEKPVEVSPAVTRGLYIAKQATGGAAKVSQLLVDGVCTVANCVGKELAPHVKKHGSKLVPESLKRDKDGKSALDGAMVVAASSVQGFSTVWQGLECAAKCIVNNVSAETVQTVRYKYGHNAGEATHNAVDSAINVGLTAYNIDNIGIKAMVKKTAKQTGHTLLEDYQIVERPQRESQGGATSTEGRRDIGKQVEEEKPGAGKKDK

Target ORF information:

RefSeq Version	NM_001144987.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus spastic paraplegia 20, spartin (Troyer syndrome) homolog (human) (Spg20), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144987.1

ORF Insert Sequence:

ATGGAGAGAG AACCAGAAAA TGGAGAACCT GCTGAAATTA AGATCATCAA GGAAGCATAC 
GAGAAGGCCT TTATGTTTGT TAATAAAGGA CTGAACACGG ACGAGTTAGG GCAGAAGGAA 
GAAGCAAAGA ACTATTACAA GCAAGGAATA GGACACCTGC TTAGGGGCAT CAGCATCGCG 
GCTGCAGAGC CGGGGCACAC AGGCCCTGCG TGGGAGGCTG CCAGGCAGAT GCAACAGAAA 
ATGAAGGAAA CGCTTCAGAA CGTCCGCACC AGGCTAGAGA TCCTGGAGAA GGGCCTTGCC 
ACTTCTCTAC GGAATGACCT TCAGGATGTG CCCAAGCTAT ACCCAGAGTT CCCGCCTAAA 
GACGCGTGCA AGAAGTCCCC AGAGCAGGAG TCCGTTAGTA CAGCGCCCCA GCGTGCTGAA 
GTCGATGGGA GCGCCTCAGC CGCGTGTGCC GGCCCAAGTG GTGCGCCTTC TGCTCTGCCC 
GTACCATCGC CCAGCTGCCC TGCCGAAGCG CCACCAGCCT ACAGCCCACA GGCTGCTGAG 
GGCCACTACA CTGTTTCCTA TGGAACAGAT TCCGGGGAGT TTTCATCCGT GGGAGAAGAC 
TTCTACAGGA ACCGTTCCCA GCCGCCCCCT CTTGAGACCT TGGGGCTCGA TGCAGATGAG 
TTGATCTTGA TACCAAACGG AGTGCAGATC TTTTTTGTAA ACCCTGCGGG AGAGGTCAGT 
GCTCCCTCGT ACCCTGGGTA TCTGCGGATC GTGCGGTTCT TGGATAATTC TCTGGATACA 
GTTCTGAACC GCCCTCCTGG GTTTCTCCAG GTGTGTGACT GGTTATACCC TCTAGTTCCT 
GATAGGTCAC CAGTTCTGAA GTGCACTGTG GGAGCCTACA TGTTTCCAGA TACCATGTTA 
CAAGCTGCTG GGTGCTTCGT AGGGGTTGTT CTTTCATCTG AGCTCCCAGA AGATGACAGA 
GAGCTGTTTG AGGATCTGTT GAGACAGATG TCTGACCTTC GGCTCCAGGC CAACTGGAAT 
AGAGAAGAAG ATGAATTCCA AATCCCTGGG AGATCAAGTC ACCCCTCTGA ACCACCGAAG 
GAAGCTTCTG GCACTGATGT CAGGCAGTCA AGTTCTTCAG GTTCCTCAAT AGACCAAGGC 
AGCAAGGATG CCCGCCATAA AGGAAAGCGT GGGAAAAAGA CCAAAGATTC AAGTGAAGAA 
GTTAATCTGA GCCAGATTGT GCCCTGTGAG CCGAGTTCAG AAGAAAAATC GAAAGAATTG 
CCTGAATGGA GCGAGAAAGT GGCCCACAAC ATCCTGTCAG GTGCTTCCTG GGTGAGCTGG 
GGTTTAGTCA AAGGTGCTGA ATTTACTGGC AAAGCAATTC AGAAAGGTGC TTCTAAACTC 
AGAGAGCGCA TTCAGCCGGA GGAAAAGCCA GTGGAGGTGA GCCCAGCTGT CACCAGGGGT 
CTCTACATAG CGAAGCAGGC AACCGGAGGA GCAGCCAAAG TCAGCCAGCT CCTGGTTGAC 
GGCGTCTGCA CAGTAGCGAA TTGTGTTGGT AAAGAACTTG CTCCACACGT CAAGAAGCAC 
GGAAGCAAAC TGGTTCCGGA ATCCCTGAAG AGGGACAAAG ACGGGAAATC TGCCCTGGAT 
GGCGCCATGG TCGTGGCAGC GAGTAGTGTT CAAGGATTCT CAACTGTCTG GCAAGGGTTG 
GAATGTGCAG CTAAATGCAT CGTTAACAAC GTGTCTGCAG AAACTGTACA GACTGTGAGA 
TACAAGTATG GGCACAACGC AGGAGAAGCT ACACATAACG CAGTGGATTC AGCTATCAAT 
GTTGGGCTCA CTGCCTACAA TATTGACAAC ATCGGCATCA AAGCCATGGT GAAGAAAACT 
GCCAAGCAGA CGGGACACAC TCTCCTCGAG GACTACCAGA TCGTTGAGCG CCCTCAGAGG 
GAGAGTCAGG GAGGAGCCAC AAGCACAGAA GGGAGAAGAG ACATAGGAAA GCAGGTGGAG 
GAAGAGAAGC CAGGGGCAGG AAAGAAGGAC AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spg20 ( NM_144895.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_659144.1 Mus musculus spastic paraplegia 20, spartin (Troyer syndrome) homolog (human) (Spg20), transcript variant 1, mRNA.

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CloneID	OMu06767
Clone ID Related Accession (Same CDS sequence)	NM_001144987.1 , XM_006501320.2 , NM_144895.2 , XM_006501320.3
Accession Version	NM_144895.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Mus musculus(house mouse)
Product	spartin isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK159951.1, AK129171.1 and BU755937.1. On Feb 3, 2009 this sequence version replaced NM_144895.1. Transcript Variant: This variant (1) encodes the longer isoform (a). Both variants 1 and 2 encode the same isoform. ##Evidence-Data-START## Transcript exon combination :: AK087388.1, SRR1660811.151045.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGAGAG AACCAGAAAA TGGAGAACCT GCTGAAATTA AGATCATCAA GGAAGCATAC 
GAGAAGGCCT TTATGTTTGT TAATAAAGGA CTGAACACGG ACGAGTTAGG GCAGAAGGAA 
GAAGCAAAGA ACTATTACAA GCAAGGAATA GGACACCTGC TTAGGGGCAT CAGCATCGCG 
GCTGCAGAGC CGGGGCACAC AGGCCCTGCG TGGGAGGCTG CCAGGCAGAT GCAACAGAAA 
ATGAAGGAAA CGCTTCAGAA CGTCCGCACC AGGCTAGAGA TCCTGGAGAA GGGCCTTGCC 
ACTTCTCTAC GGAATGACCT TCAGGATGTG CCCAAGCTAT ACCCAGAGTT CCCGCCTAAA 
GACGCGTGCA AGAAGTCCCC AGAGCAGGAG TCCGTTAGTA CAGCGCCCCA GCGTGCTGAA 
GTCGATGGGA GCGCCTCAGC CGCGTGTGCC GGCCCAAGTG GTGCGCCTTC TGCTCTGCCC 
GTACCATCGC CCAGCTGCCC TGCCGAAGCG CCACCAGCCT ACAGCCCACA GGCTGCTGAG 
GGCCACTACA CTGTTTCCTA TGGAACAGAT TCCGGGGAGT TTTCATCCGT GGGAGAAGAC 
TTCTACAGGA ACCGTTCCCA GCCGCCCCCT CTTGAGACCT TGGGGCTCGA TGCAGATGAG 
TTGATCTTGA TACCAAACGG AGTGCAGATC TTTTTTGTAA ACCCTGCGGG AGAGGTCAGT 
GCTCCCTCGT ACCCTGGGTA TCTGCGGATC GTGCGGTTCT TGGATAATTC TCTGGATACA 
GTTCTGAACC GCCCTCCTGG GTTTCTCCAG GTGTGTGACT GGTTATACCC TCTAGTTCCT 
GATAGGTCAC CAGTTCTGAA GTGCACTGTG GGAGCCTACA TGTTTCCAGA TACCATGTTA 
CAAGCTGCTG GGTGCTTCGT AGGGGTTGTT CTTTCATCTG AGCTCCCAGA AGATGACAGA 
GAGCTGTTTG AGGATCTGTT GAGACAGATG TCTGACCTTC GGCTCCAGGC CAACTGGAAT 
AGAGAAGAAG ATGAATTCCA AATCCCTGGG AGATCAAGTC ACCCCTCTGA ACCACCGAAG 
GAAGCTTCTG GCACTGATGT CAGGCAGTCA AGTTCTTCAG GTTCCTCAAT AGACCAAGGC 
AGCAAGGATG CCCGCCATAA AGGAAAGCGT GGGAAAAAGA CCAAAGATTC AAGTGAAGAA 
GTTAATCTGA GCCAGATTGT GCCCTGTGAG CCGAGTTCAG AAGAAAAATC GAAAGAATTG 
CCTGAATGGA GCGAGAAAGT GGCCCACAAC ATCCTGTCAG GTGCTTCCTG GGTGAGCTGG 
GGTTTAGTCA AAGGTGCTGA ATTTACTGGC AAAGCAATTC AGAAAGGTGC TTCTAAACTC 
AGAGAGCGCA TTCAGCCGGA GGAAAAGCCA GTGGAGGTGA GCCCAGCTGT CACCAGGGGT 
CTCTACATAG CGAAGCAGGC AACCGGAGGA GCAGCCAAAG TCAGCCAGCT CCTGGTTGAC 
GGCGTCTGCA CAGTAGCGAA TTGTGTTGGT AAAGAACTTG CTCCACACGT CAAGAAGCAC 
GGAAGCAAAC TGGTTCCGGA ATCCCTGAAG AGGGACAAAG ACGGGAAATC TGCCCTGGAT 
GGCGCCATGG TCGTGGCAGC GAGTAGTGTT CAAGGATTCT CAACTGTCTG GCAAGGGTTG 
GAATGTGCAG CTAAATGCAT CGTTAACAAC GTGTCTGCAG AAACTGTACA GACTGTGAGA 
TACAAGTATG GGCACAACGC AGGAGAAGCT ACACATAACG CAGTGGATTC AGCTATCAAT 
GTTGGGCTCA CTGCCTACAA TATTGACAAC ATCGGCATCA AAGCCATGGT GAAGAAAACT 
GCCAAGCAGA CGGGACACAC TCTCCTCGAG GACTACCAGA TCGTTGAGCG CCCTCAGAGG 
GAGAGTCAGG GAGGAGCCAC AAGCACAGAA GGGAGAAGAG ACATAGGAAA GCAGGTGGAG 
GAAGAGAAGC CAGGGGCAGG AAAGAAGGAC AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_659144.1
CDS	113..2128
Translation	MEREPENGEPAEIKIIKEAYEKAFMFVNKGLNTDELGQKEEAKNYYKQGIGHLLRGISIAAAEPGHTGPAWEAARQMQQKMKETLQNVRTRLEILEKGLATSLRNDLQDVPKLYPEFPPKDACKKSPEQESVSTAPQRAEVDGSASAACAGPSGAPSALPVPSPSCPAEAPPAYSPQAAEGHYTVSYGTDSGEFSSVGEDFYRNRSQPPPLETLGLDADELILIPNGVQIFFVNPAGEVSAPSYPGYLRIVRFLDNSLDTVLNRPPGFLQVCDWLYPLVPDRSPVLKCTVGAYMFPDTMLQAAGCFVGVVLSSELPEDDRELFEDLLRQMSDLRLQANWNREEDEFQIPGRSSHPSEPPKEASGTDVRQSSSSGSSIDQGSKDARHKGKRGKKTKDSSEEVNLSQIVPCEPSSEEKSKELPEWSEKVAHNILSGASWVSWGLVKGAEFTGKAIQKGASKLRERIQPEEKPVEVSPAVTRGLYIAKQATGGAAKVSQLLVDGVCTVANCVGKELAPHVKKHGSKLVPESLKRDKDGKSALDGAMVVAASSVQGFSTVWQGLECAAKCIVNNVSAETVQTVRYKYGHNAGEATHNAVDSAINVGLTAYNIDNIGIKAMVKKTAKQTGHTLLEDYQIVERPQRESQGGATSTEGRRDIGKQVEEEKPGAGKKDK

Target ORF information:

RefSeq Version	NM_144895.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus spastic paraplegia 20, spartin (Troyer syndrome) homolog (human) (Spg20), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_144895.2

ORF Insert Sequence:

ATGGAGAGAG AACCAGAAAA TGGAGAACCT GCTGAAATTA AGATCATCAA GGAAGCATAC 
GAGAAGGCCT TTATGTTTGT TAATAAAGGA CTGAACACGG ACGAGTTAGG GCAGAAGGAA 
GAAGCAAAGA ACTATTACAA GCAAGGAATA GGACACCTGC TTAGGGGCAT CAGCATCGCG 
GCTGCAGAGC CGGGGCACAC AGGCCCTGCG TGGGAGGCTG CCAGGCAGAT GCAACAGAAA 
ATGAAGGAAA CGCTTCAGAA CGTCCGCACC AGGCTAGAGA TCCTGGAGAA GGGCCTTGCC 
ACTTCTCTAC GGAATGACCT TCAGGATGTG CCCAAGCTAT ACCCAGAGTT CCCGCCTAAA 
GACGCGTGCA AGAAGTCCCC AGAGCAGGAG TCCGTTAGTA CAGCGCCCCA GCGTGCTGAA 
GTCGATGGGA GCGCCTCAGC CGCGTGTGCC GGCCCAAGTG GTGCGCCTTC TGCTCTGCCC 
GTACCATCGC CCAGCTGCCC TGCCGAAGCG CCACCAGCCT ACAGCCCACA GGCTGCTGAG 
GGCCACTACA CTGTTTCCTA TGGAACAGAT TCCGGGGAGT TTTCATCCGT GGGAGAAGAC 
TTCTACAGGA ACCGTTCCCA GCCGCCCCCT CTTGAGACCT TGGGGCTCGA TGCAGATGAG 
TTGATCTTGA TACCAAACGG AGTGCAGATC TTTTTTGTAA ACCCTGCGGG AGAGGTCAGT 
GCTCCCTCGT ACCCTGGGTA TCTGCGGATC GTGCGGTTCT TGGATAATTC TCTGGATACA 
GTTCTGAACC GCCCTCCTGG GTTTCTCCAG GTGTGTGACT GGTTATACCC TCTAGTTCCT 
GATAGGTCAC CAGTTCTGAA GTGCACTGTG GGAGCCTACA TGTTTCCAGA TACCATGTTA 
CAAGCTGCTG GGTGCTTCGT AGGGGTTGTT CTTTCATCTG AGCTCCCAGA AGATGACAGA 
GAGCTGTTTG AGGATCTGTT GAGACAGATG TCTGACCTTC GGCTCCAGGC CAACTGGAAT 
AGAGAAGAAG ATGAATTCCA AATCCCTGGG AGATCAAGTC ACCCCTCTGA ACCACCGAAG 
GAAGCTTCTG GCACTGATGT CAGGCAGTCA AGTTCTTCAG GTTCCTCAAT AGACCAAGGC 
AGCAAGGATG CCCGCCATAA AGGAAAGCGT GGGAAAAAGA CCAAAGATTC AAGTGAAGAA 
GTTAATCTGA GCCAGATTGT GCCCTGTGAG CCGAGTTCAG AAGAAAAATC GAAAGAATTG 
CCTGAATGGA GCGAGAAAGT GGCCCACAAC ATCCTGTCAG GTGCTTCCTG GGTGAGCTGG 
GGTTTAGTCA AAGGTGCTGA ATTTACTGGC AAAGCAATTC AGAAAGGTGC TTCTAAACTC 
AGAGAGCGCA TTCAGCCGGA GGAAAAGCCA GTGGAGGTGA GCCCAGCTGT CACCAGGGGT 
CTCTACATAG CGAAGCAGGC AACCGGAGGA GCAGCCAAAG TCAGCCAGCT CCTGGTTGAC 
GGCGTCTGCA CAGTAGCGAA TTGTGTTGGT AAAGAACTTG CTCCACACGT CAAGAAGCAC 
GGAAGCAAAC TGGTTCCGGA ATCCCTGAAG AGGGACAAAG ACGGGAAATC TGCCCTGGAT 
GGCGCCATGG TCGTGGCAGC GAGTAGTGTT CAAGGATTCT CAACTGTCTG GCAAGGGTTG 
GAATGTGCAG CTAAATGCAT CGTTAACAAC GTGTCTGCAG AAACTGTACA GACTGTGAGA 
TACAAGTATG GGCACAACGC AGGAGAAGCT ACACATAACG CAGTGGATTC AGCTATCAAT 
GTTGGGCTCA CTGCCTACAA TATTGACAAC ATCGGCATCA AAGCCATGGT GAAGAAAACT 
GCCAAGCAGA CGGGACACAC TCTCCTCGAG GACTACCAGA TCGTTGAGCG CCCTCAGAGG 
GAGAGTCAGG GAGGAGCCAC AAGCACAGAA GGGAGAAGAG ACATAGGAAA GCAGGTGGAG 
GAAGAGAAGC CAGGGGCAGG AAAGAAGGAC AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spg20 ( XM_006501320.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501383.1 Mus musculus spastic paraplegia 20, spartin (Troyer syndrome) homolog (human) (Spg20), transcript variant X1, mRNA.

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CloneID	OMu06767
Clone ID Related Accession (Same CDS sequence)	NM_001144987.1 , XM_006501320.2 , NM_144895.2 , XM_006501320.3
Accession Version	XM_006501320.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	spartin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006501320.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGAG AACCAGAAAA TGGAGAACCT GCTGAAATTA AGATCATCAA GGAAGCATAC 
GAGAAGGCCT TTATGTTTGT TAATAAAGGA CTGAACACGG ACGAGTTAGG GCAGAAGGAA 
GAAGCAAAGA ACTATTACAA GCAAGGAATA GGACACCTGC TTAGGGGCAT CAGCATCGCG 
GCTGCAGAGC CGGGGCACAC AGGCCCTGCG TGGGAGGCTG CCAGGCAGAT GCAACAGAAA 
ATGAAGGAAA CGCTTCAGAA CGTCCGCACC AGGCTAGAGA TCCTGGAGAA GGGCCTTGCC 
ACTTCTCTAC GGAATGACCT TCAGGATGTG CCCAAGCTAT ACCCAGAGTT CCCGCCTAAA 
GACGCGTGCA AGAAGTCCCC AGAGCAGGAG TCCGTTAGTA CAGCGCCCCA GCGTGCTGAA 
GTCGATGGGA GCGCCTCAGC CGCGTGTGCC GGCCCAAGTG GTGCGCCTTC TGCTCTGCCC 
GTACCATCGC CCAGCTGCCC TGCCGAAGCG CCACCAGCCT ACAGCCCACA GGCTGCTGAG 
GGCCACTACA CTGTTTCCTA TGGAACAGAT TCCGGGGAGT TTTCATCCGT GGGAGAAGAC 
TTCTACAGGA ACCGTTCCCA GCCGCCCCCT CTTGAGACCT TGGGGCTCGA TGCAGATGAG 
TTGATCTTGA TACCAAACGG AGTGCAGATC TTTTTTGTAA ACCCTGCGGG AGAGGTCAGT 
GCTCCCTCGT ACCCTGGGTA TCTGCGGATC GTGCGGTTCT TGGATAATTC TCTGGATACA 
GTTCTGAACC GCCCTCCTGG GTTTCTCCAG GTGTGTGACT GGTTATACCC TCTAGTTCCT 
GATAGGTCAC CAGTTCTGAA GTGCACTGTG GGAGCCTACA TGTTTCCAGA TACCATGTTA 
CAAGCTGCTG GGTGCTTCGT AGGGGTTGTT CTTTCATCTG AGCTCCCAGA AGATGACAGA 
GAGCTGTTTG AGGATCTGTT GAGACAGATG TCTGACCTTC GGCTCCAGGC CAACTGGAAT 
AGAGAAGAAG ATGAATTCCA AATCCCTGGG AGATCAAGTC ACCCCTCTGA ACCACCGAAG 
GAAGCTTCTG GCACTGATGT CAGGCAGTCA AGTTCTTCAG GTTCCTCAAT AGACCAAGGC 
AGCAAGGATG CCCGCCATAA AGGAAAGCGT GGGAAAAAGA CCAAAGATTC AAGTGAAGAA 
GTTAATCTGA GCCAGATTGT GCCCTGTGAG CCGAGTTCAG AAGAAAAATC GAAAGAATTG 
CCTGAATGGA GCGAGAAAGT GGCCCACAAC ATCCTGTCAG GTGCTTCCTG GGTGAGCTGG 
GGTTTAGTCA AAGGTGCTGA ATTTACTGGC AAAGCAATTC AGAAAGGTGC TTCTAAACTC 
AGAGAGCGCA TTCAGCCGGA GGAAAAGCCA GTGGAGGTGA GCCCAGCTGT CACCAGGGGT 
CTCTACATAG CGAAGCAGGC AACCGGAGGA GCAGCCAAAG TCAGCCAGCT CCTGGTTGAC 
GGCGTCTGCA CAGTAGCGAA TTGTGTTGGT AAAGAACTTG CTCCACACGT CAAGAAGCAC 
GGAAGCAAAC TGGTTCCGGA ATCCCTGAAG AGGGACAAAG ACGGGAAATC TGCCCTGGAT 
GGCGCCATGG TCGTGGCAGC GAGTAGTGTT CAAGGATTCT CAACTGTCTG GCAAGGGTTG 
GAATGTGCAG CTAAATGCAT CGTTAACAAC GTGTCTGCAG AAACTGTACA GACTGTGAGA 
TACAAGTATG GGCACAACGC AGGAGAAGCT ACACATAACG CAGTGGATTC AGCTATCAAT 
GTTGGGCTCA CTGCCTACAA TATTGACAAC ATCGGCATCA AAGCCATGGT GAAGAAAACT 
GCCAAGCAGA CGGGACACAC TCTCCTCGAG GACTACCAGA TCGTTGAGCG CCCTCAGAGG 
GAGAGTCAGG GAGGAGCCAC AAGCACAGAA GGGAGAAGAG ACATAGGAAA GCAGGTGGAG 
GAAGAGAAGC CAGGGGCAGG AAAGAAGGAC AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501383.1
CDS	236..2251
Translation	MEREPENGEPAEIKIIKEAYEKAFMFVNKGLNTDELGQKEEAKNYYKQGIGHLLRGISIAAAEPGHTGPAWEAARQMQQKMKETLQNVRTRLEILEKGLATSLRNDLQDVPKLYPEFPPKDACKKSPEQESVSTAPQRAEVDGSASAACAGPSGAPSALPVPSPSCPAEAPPAYSPQAAEGHYTVSYGTDSGEFSSVGEDFYRNRSQPPPLETLGLDADELILIPNGVQIFFVNPAGEVSAPSYPGYLRIVRFLDNSLDTVLNRPPGFLQVCDWLYPLVPDRSPVLKCTVGAYMFPDTMLQAAGCFVGVVLSSELPEDDRELFEDLLRQMSDLRLQANWNREEDEFQIPGRSSHPSEPPKEASGTDVRQSSSSGSSIDQGSKDARHKGKRGKKTKDSSEEVNLSQIVPCEPSSEEKSKELPEWSEKVAHNILSGASWVSWGLVKGAEFTGKAIQKGASKLRERIQPEEKPVEVSPAVTRGLYIAKQATGGAAKVSQLLVDGVCTVANCVGKELAPHVKKHGSKLVPESLKRDKDGKSALDGAMVVAASSVQGFSTVWQGLECAAKCIVNNVSAETVQTVRYKYGHNAGEATHNAVDSAINVGLTAYNIDNIGIKAMVKKTAKQTGHTLLEDYQIVERPQRESQGGATSTEGRRDIGKQVEEEKPGAGKKDK

Target ORF information:

RefSeq Version	XM_006501320.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus spastic paraplegia 20, spartin (Troyer syndrome) homolog (human) (Spg20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501320.2

ORF Insert Sequence:

ATGGAGAGAG AACCAGAAAA TGGAGAACCT GCTGAAATTA AGATCATCAA GGAAGCATAC 
GAGAAGGCCT TTATGTTTGT TAATAAAGGA CTGAACACGG ACGAGTTAGG GCAGAAGGAA 
GAAGCAAAGA ACTATTACAA GCAAGGAATA GGACACCTGC TTAGGGGCAT CAGCATCGCG 
GCTGCAGAGC CGGGGCACAC AGGCCCTGCG TGGGAGGCTG CCAGGCAGAT GCAACAGAAA 
ATGAAGGAAA CGCTTCAGAA CGTCCGCACC AGGCTAGAGA TCCTGGAGAA GGGCCTTGCC 
ACTTCTCTAC GGAATGACCT TCAGGATGTG CCCAAGCTAT ACCCAGAGTT CCCGCCTAAA 
GACGCGTGCA AGAAGTCCCC AGAGCAGGAG TCCGTTAGTA CAGCGCCCCA GCGTGCTGAA 
GTCGATGGGA GCGCCTCAGC CGCGTGTGCC GGCCCAAGTG GTGCGCCTTC TGCTCTGCCC 
GTACCATCGC CCAGCTGCCC TGCCGAAGCG CCACCAGCCT ACAGCCCACA GGCTGCTGAG 
GGCCACTACA CTGTTTCCTA TGGAACAGAT TCCGGGGAGT TTTCATCCGT GGGAGAAGAC 
TTCTACAGGA ACCGTTCCCA GCCGCCCCCT CTTGAGACCT TGGGGCTCGA TGCAGATGAG 
TTGATCTTGA TACCAAACGG AGTGCAGATC TTTTTTGTAA ACCCTGCGGG AGAGGTCAGT 
GCTCCCTCGT ACCCTGGGTA TCTGCGGATC GTGCGGTTCT TGGATAATTC TCTGGATACA 
GTTCTGAACC GCCCTCCTGG GTTTCTCCAG GTGTGTGACT GGTTATACCC TCTAGTTCCT 
GATAGGTCAC CAGTTCTGAA GTGCACTGTG GGAGCCTACA TGTTTCCAGA TACCATGTTA 
CAAGCTGCTG GGTGCTTCGT AGGGGTTGTT CTTTCATCTG AGCTCCCAGA AGATGACAGA 
GAGCTGTTTG AGGATCTGTT GAGACAGATG TCTGACCTTC GGCTCCAGGC CAACTGGAAT 
AGAGAAGAAG ATGAATTCCA AATCCCTGGG AGATCAAGTC ACCCCTCTGA ACCACCGAAG 
GAAGCTTCTG GCACTGATGT CAGGCAGTCA AGTTCTTCAG GTTCCTCAAT AGACCAAGGC 
AGCAAGGATG CCCGCCATAA AGGAAAGCGT GGGAAAAAGA CCAAAGATTC AAGTGAAGAA 
GTTAATCTGA GCCAGATTGT GCCCTGTGAG CCGAGTTCAG AAGAAAAATC GAAAGAATTG 
CCTGAATGGA GCGAGAAAGT GGCCCACAAC ATCCTGTCAG GTGCTTCCTG GGTGAGCTGG 
GGTTTAGTCA AAGGTGCTGA ATTTACTGGC AAAGCAATTC AGAAAGGTGC TTCTAAACTC 
AGAGAGCGCA TTCAGCCGGA GGAAAAGCCA GTGGAGGTGA GCCCAGCTGT CACCAGGGGT 
CTCTACATAG CGAAGCAGGC AACCGGAGGA GCAGCCAAAG TCAGCCAGCT CCTGGTTGAC 
GGCGTCTGCA CAGTAGCGAA TTGTGTTGGT AAAGAACTTG CTCCACACGT CAAGAAGCAC 
GGAAGCAAAC TGGTTCCGGA ATCCCTGAAG AGGGACAAAG ACGGGAAATC TGCCCTGGAT 
GGCGCCATGG TCGTGGCAGC GAGTAGTGTT CAAGGATTCT CAACTGTCTG GCAAGGGTTG 
GAATGTGCAG CTAAATGCAT CGTTAACAAC GTGTCTGCAG AAACTGTACA GACTGTGAGA 
TACAAGTATG GGCACAACGC AGGAGAAGCT ACACATAACG CAGTGGATTC AGCTATCAAT 
GTTGGGCTCA CTGCCTACAA TATTGACAAC ATCGGCATCA AAGCCATGGT GAAGAAAACT 
GCCAAGCAGA CGGGACACAC TCTCCTCGAG GACTACCAGA TCGTTGAGCG CCCTCAGAGG 
GAGAGTCAGG GAGGAGCCAC AAGCACAGAA GGGAGAAGAG ACATAGGAAA GCAGGTGGAG 
GAAGAGAAGC CAGGGGCAGG AAAGAAGGAC AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spg20 ( XM_006501320.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006501383.1 Mus musculus spastic paraplegia 20, spartin (Troyer syndrome) homolog (human) (Spg20), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu06767
Clone ID Related Accession (Same CDS sequence)	NM_001144987.1 , XM_006501320.2 , NM_144895.2 , XM_006501320.3
Accession Version	XM_006501320.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	spartin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006501320.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGAG AACCAGAAAA TGGAGAACCT GCTGAAATTA AGATCATCAA GGAAGCATAC 
GAGAAGGCCT TTATGTTTGT TAATAAAGGA CTGAACACGG ACGAGTTAGG GCAGAAGGAA 
GAAGCAAAGA ACTATTACAA GCAAGGAATA GGACACCTGC TTAGGGGCAT CAGCATCGCG 
GCTGCAGAGC CGGGGCACAC AGGCCCTGCG TGGGAGGCTG CCAGGCAGAT GCAACAGAAA 
ATGAAGGAAA CGCTTCAGAA CGTCCGCACC AGGCTAGAGA TCCTGGAGAA GGGCCTTGCC 
ACTTCTCTAC GGAATGACCT TCAGGATGTG CCCAAGCTAT ACCCAGAGTT CCCGCCTAAA 
GACGCGTGCA AGAAGTCCCC AGAGCAGGAG TCCGTTAGTA CAGCGCCCCA GCGTGCTGAA 
GTCGATGGGA GCGCCTCAGC CGCGTGTGCC GGCCCAAGTG GTGCGCCTTC TGCTCTGCCC 
GTACCATCGC CCAGCTGCCC TGCCGAAGCG CCACCAGCCT ACAGCCCACA GGCTGCTGAG 
GGCCACTACA CTGTTTCCTA TGGAACAGAT TCCGGGGAGT TTTCATCCGT GGGAGAAGAC 
TTCTACAGGA ACCGTTCCCA GCCGCCCCCT CTTGAGACCT TGGGGCTCGA TGCAGATGAG 
TTGATCTTGA TACCAAACGG AGTGCAGATC TTTTTTGTAA ACCCTGCGGG AGAGGTCAGT 
GCTCCCTCGT ACCCTGGGTA TCTGCGGATC GTGCGGTTCT TGGATAATTC TCTGGATACA 
GTTCTGAACC GCCCTCCTGG GTTTCTCCAG GTGTGTGACT GGTTATACCC TCTAGTTCCT 
GATAGGTCAC CAGTTCTGAA GTGCACTGTG GGAGCCTACA TGTTTCCAGA TACCATGTTA 
CAAGCTGCTG GGTGCTTCGT AGGGGTTGTT CTTTCATCTG AGCTCCCAGA AGATGACAGA 
GAGCTGTTTG AGGATCTGTT GAGACAGATG TCTGACCTTC GGCTCCAGGC CAACTGGAAT 
AGAGAAGAAG ATGAATTCCA AATCCCTGGG AGATCAAGTC ACCCCTCTGA ACCACCGAAG 
GAAGCTTCTG GCACTGATGT CAGGCAGTCA AGTTCTTCAG GTTCCTCAAT AGACCAAGGC 
AGCAAGGATG CCCGCCATAA AGGAAAGCGT GGGAAAAAGA CCAAAGATTC AAGTGAAGAA 
GTTAATCTGA GCCAGATTGT GCCCTGTGAG CCGAGTTCAG AAGAAAAATC GAAAGAATTG 
CCTGAATGGA GCGAGAAAGT GGCCCACAAC ATCCTGTCAG GTGCTTCCTG GGTGAGCTGG 
GGTTTAGTCA AAGGTGCTGA ATTTACTGGC AAAGCAATTC AGAAAGGTGC TTCTAAACTC 
AGAGAGCGCA TTCAGCCGGA GGAAAAGCCA GTGGAGGTGA GCCCAGCTGT CACCAGGGGT 
CTCTACATAG CGAAGCAGGC AACCGGAGGA GCAGCCAAAG TCAGCCAGCT CCTGGTTGAC 
GGCGTCTGCA CAGTAGCGAA TTGTGTTGGT AAAGAACTTG CTCCACACGT CAAGAAGCAC 
GGAAGCAAAC TGGTTCCGGA ATCCCTGAAG AGGGACAAAG ACGGGAAATC TGCCCTGGAT 
GGCGCCATGG TCGTGGCAGC GAGTAGTGTT CAAGGATTCT CAACTGTCTG GCAAGGGTTG 
GAATGTGCAG CTAAATGCAT CGTTAACAAC GTGTCTGCAG AAACTGTACA GACTGTGAGA 
TACAAGTATG GGCACAACGC AGGAGAAGCT ACACATAACG CAGTGGATTC AGCTATCAAT 
GTTGGGCTCA CTGCCTACAA TATTGACAAC ATCGGCATCA AAGCCATGGT GAAGAAAACT 
GCCAAGCAGA CGGGACACAC TCTCCTCGAG GACTACCAGA TCGTTGAGCG CCCTCAGAGG 
GAGAGTCAGG GAGGAGCCAC AAGCACAGAA GGGAGAAGAG ACATAGGAAA GCAGGTGGAG 
GAAGAGAAGC CAGGGGCAGG AAAGAAGGAC AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006501383.1
CDS	188..2203
Translation	MEREPENGEPAEIKIIKEAYEKAFMFVNKGLNTDELGQKEEAKNYYKQGIGHLLRGISIAAAEPGHTGPAWEAARQMQQKMKETLQNVRTRLEILEKGLATSLRNDLQDVPKLYPEFPPKDACKKSPEQESVSTAPQRAEVDGSASAACAGPSGAPSALPVPSPSCPAEAPPAYSPQAAEGHYTVSYGTDSGEFSSVGEDFYRNRSQPPPLETLGLDADELILIPNGVQIFFVNPAGEVSAPSYPGYLRIVRFLDNSLDTVLNRPPGFLQVCDWLYPLVPDRSPVLKCTVGAYMFPDTMLQAAGCFVGVVLSSELPEDDRELFEDLLRQMSDLRLQANWNREEDEFQIPGRSSHPSEPPKEASGTDVRQSSSSGSSIDQGSKDARHKGKRGKKTKDSSEEVNLSQIVPCEPSSEEKSKELPEWSEKVAHNILSGASWVSWGLVKGAEFTGKAIQKGASKLRERIQPEEKPVEVSPAVTRGLYIAKQATGGAAKVSQLLVDGVCTVANCVGKELAPHVKKHGSKLVPESLKRDKDGKSALDGAMVVAASSVQGFSTVWQGLECAAKCIVNNVSAETVQTVRYKYGHNAGEATHNAVDSAINVGLTAYNIDNIGIKAMVKKTAKQTGHTLLEDYQIVERPQRESQGGATSTEGRRDIGKQVEEEKPGAGKKDK

Target ORF information:

RefSeq Version	XM_006501320.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus spastic paraplegia 20, spartin (Troyer syndrome) homolog (human) (Spg20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006501320.3

ORF Insert Sequence:

ATGGAGAGAG AACCAGAAAA TGGAGAACCT GCTGAAATTA AGATCATCAA GGAAGCATAC 
GAGAAGGCCT TTATGTTTGT TAATAAAGGA CTGAACACGG ACGAGTTAGG GCAGAAGGAA 
GAAGCAAAGA ACTATTACAA GCAAGGAATA GGACACCTGC TTAGGGGCAT CAGCATCGCG 
GCTGCAGAGC CGGGGCACAC AGGCCCTGCG TGGGAGGCTG CCAGGCAGAT GCAACAGAAA 
ATGAAGGAAA CGCTTCAGAA CGTCCGCACC AGGCTAGAGA TCCTGGAGAA GGGCCTTGCC 
ACTTCTCTAC GGAATGACCT TCAGGATGTG CCCAAGCTAT ACCCAGAGTT CCCGCCTAAA 
GACGCGTGCA AGAAGTCCCC AGAGCAGGAG TCCGTTAGTA CAGCGCCCCA GCGTGCTGAA 
GTCGATGGGA GCGCCTCAGC CGCGTGTGCC GGCCCAAGTG GTGCGCCTTC TGCTCTGCCC 
GTACCATCGC CCAGCTGCCC TGCCGAAGCG CCACCAGCCT ACAGCCCACA GGCTGCTGAG 
GGCCACTACA CTGTTTCCTA TGGAACAGAT TCCGGGGAGT TTTCATCCGT GGGAGAAGAC 
TTCTACAGGA ACCGTTCCCA GCCGCCCCCT CTTGAGACCT TGGGGCTCGA TGCAGATGAG 
TTGATCTTGA TACCAAACGG AGTGCAGATC TTTTTTGTAA ACCCTGCGGG AGAGGTCAGT 
GCTCCCTCGT ACCCTGGGTA TCTGCGGATC GTGCGGTTCT TGGATAATTC TCTGGATACA 
GTTCTGAACC GCCCTCCTGG GTTTCTCCAG GTGTGTGACT GGTTATACCC TCTAGTTCCT 
GATAGGTCAC CAGTTCTGAA GTGCACTGTG GGAGCCTACA TGTTTCCAGA TACCATGTTA 
CAAGCTGCTG GGTGCTTCGT AGGGGTTGTT CTTTCATCTG AGCTCCCAGA AGATGACAGA 
GAGCTGTTTG AGGATCTGTT GAGACAGATG TCTGACCTTC GGCTCCAGGC CAACTGGAAT 
AGAGAAGAAG ATGAATTCCA AATCCCTGGG AGATCAAGTC ACCCCTCTGA ACCACCGAAG 
GAAGCTTCTG GCACTGATGT CAGGCAGTCA AGTTCTTCAG GTTCCTCAAT AGACCAAGGC 
AGCAAGGATG CCCGCCATAA AGGAAAGCGT GGGAAAAAGA CCAAAGATTC AAGTGAAGAA 
GTTAATCTGA GCCAGATTGT GCCCTGTGAG CCGAGTTCAG AAGAAAAATC GAAAGAATTG 
CCTGAATGGA GCGAGAAAGT GGCCCACAAC ATCCTGTCAG GTGCTTCCTG GGTGAGCTGG 
GGTTTAGTCA AAGGTGCTGA ATTTACTGGC AAAGCAATTC AGAAAGGTGC TTCTAAACTC 
AGAGAGCGCA TTCAGCCGGA GGAAAAGCCA GTGGAGGTGA GCCCAGCTGT CACCAGGGGT 
CTCTACATAG CGAAGCAGGC AACCGGAGGA GCAGCCAAAG TCAGCCAGCT CCTGGTTGAC 
GGCGTCTGCA CAGTAGCGAA TTGTGTTGGT AAAGAACTTG CTCCACACGT CAAGAAGCAC 
GGAAGCAAAC TGGTTCCGGA ATCCCTGAAG AGGGACAAAG ACGGGAAATC TGCCCTGGAT 
GGCGCCATGG TCGTGGCAGC GAGTAGTGTT CAAGGATTCT CAACTGTCTG GCAAGGGTTG 
GAATGTGCAG CTAAATGCAT CGTTAACAAC GTGTCTGCAG AAACTGTACA GACTGTGAGA 
TACAAGTATG GGCACAACGC AGGAGAAGCT ACACATAACG CAGTGGATTC AGCTATCAAT 
GTTGGGCTCA CTGCCTACAA TATTGACAAC ATCGGCATCA AAGCCATGGT GAAGAAAACT 
GCCAAGCAGA CGGGACACAC TCTCCTCGAG GACTACCAGA TCGTTGAGCG CCCTCAGAGG 
GAGAGTCAGG GAGGAGCCAC AAGCACAGAA GGGAGAAGAG ACATAGGAAA GCAGGTGGAG 
GAAGAGAAGC CAGGGGCAGG AAAGAAGGAC AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spg20 ( XM_030252562.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030108422.1 Mus musculus spastic paraplegia 20, spartin (Troyer syndrome) homolog (human) (Spg20), transcript variant X2, mRNA.

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CloneID	OMu06769
Clone ID Related Accession (Same CDS sequence)	XM_030252562.1 , NM_001144988.1
Accession Version	XM_030252562.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	spartin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGAG AACCAGAAAA TGGAGAACCT GCTGAAATTA AGATCATCAA GGAAGCATAC 
GAGAAGGCCT TTATGTTTGT TAATAAAGGA CTGAACACGG ACGAGTTAGG GCAGAAGGAA 
GAAGCAAAGA ACTATTACAA GCAAGGAATA GGACACCTGC TTAGGGGCAT CAGCATCGCG 
GCTGCAGAGC CGGGGCACAC AGGCCCTGCG TGGGAGGCTG CCAGGCAGAT GCAACAGAAA 
ATGAAGGAAA CGCTTCAGAA CGTCCGCACC AGGCTAGAGA TCCTGGAGAA GGGCCTTGCC 
ACTTCTCTAC GGAATGACCT TCAGGATGTG CCCAAGCTAT ACCCAGAGTT CCCGCCTAAA 
GACGCGTGCA AGAAGTCCCC AGAGCAGGAG TCCGTTAGTA CAGCGCCCCA GCGTGCTGAA 
GTCGATGGGA GCGCCTCAGC CGCGTGTGCC GGCCCAAGTG GTGCGCCTTC TGCTCTGCCC 
GTACCATCGC CCAGCTGCCC TGCCGAAGCG CCACCAGCCT ACAGCCCACA GGCTGCTGAG 
GGCCACTACA CTGTTTCCTA TGGAACAGAT TCCGGGGAGT TTTCATCCGT GGGAGAAGAC 
TTCTACAGGA ACCGTTCCCA GCCGCCCCCT CTTGAGACCT TGGGGCTCGA TGCAGATGAG 
TTGATCTTGA TACCAAACGG AGTGCAGATC TTTTTTGTAA ACCCTGCGGG AGAGGTCAGT 
GCTCCCTCGT ACCCTGGGTA TCTGCGGATC GTGCGGTTCT TGGATAATTC TCTGGATACA 
GTTCTGAACC GCCCTCCTGG GTTTCTCCAG GTGTGTGACT GGTTATACCC TCTAGTTCCT 
GATAGGTCAC CAGTTCTGAA GTGCACTGTG GGAGCCTACA TGTTTCCAGA TACCATGTTA 
CAAGCTGCTG GGTGCTTCGT AGGGGTTGTT CTTTCATCTG AGCTCCCAGA AGATGACAGA 
GAGCTGTTTG AGGATCTGTT GAGACAGATG TCTGACCTTC GGCTCCAGAC CAAAGATTCA 
AGTGAAGAAG TTAATCTGAG CCAGATTGTG CCCTGTGAGC CGAGTTCAGA AGAAAAATCG 
AAAGAATTGC CTGAATGGAG CGAGAAAGTG GCCCACAACA TCCTGTCAGG TGCTTCCTGG 
GTGAGCTGGG GTTTAGTCAA AGGTGCTGAA TTTACTGGCA AAGCAATTCA GAAAGGTGCT 
TCTAAACTCA GAGAGCGCAT TCAGCCGGAG GAAAAGCCAG TGGAGGTGAG CCCAGCTGTC 
ACCAGGGGTC TCTACATAGC GAAGCAGGCA ACCGGAGGAG CAGCCAAAGT CAGCCAGCTC 
CTGGTTGACG GCGTCTGCAC AGTAGCGAAT TGTGTTGGTA AAGAACTTGC TCCACACGTC 
AAGAAGCACG GAAGCAAACT GGTTCCGGAA TCCCTGAAGA GGGACAAAGA CGGGAAATCT 
GCCCTGGATG GCGCCATGGT CGTGGCAGCG AGTAGTGTTC AAGGATTCTC AACTGTCTGG 
CAAGGGTTGG AATGTGCAGC TAAATGCATC GTTAACAACG TGTCTGCAGA AACTGTACAG 
ACTGTGAGAT ACAAGTATGG GCACAACGCA GGAGAAGCTA CACATAACGC AGTGGATTCA 
GCTATCAATG TTGGGCTCAC TGCCTACAAT ATTGACAACA TCGGCATCAA AGCCATGGTG 
AAGAAAACTG CCAAGCAGAC GGGACACACT CTCCTCGAGG ACTACCAGAT CGTTGAGCGC 
CCTCAGAGGG AGAGTCAGGG AGGAGCCACA AGCACAGAAG GGAGAAGAGA CATAGGAAAG 
CAGGTGGAGG AAGAGAAGCC AGGGGCAGGA AAGAAGGACA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030108422.1
CDS	306..2150
Translation	MEREPENGEPAEIKIIKEAYEKAFMFVNKGLNTDELGQKEEAKNYYKQGIGHLLRGISIAAAEPGHTGPAWEAARQMQQKMKETLQNVRTRLEILEKGLATSLRNDLQDVPKLYPEFPPKDACKKSPEQESVSTAPQRAEVDGSASAACAGPSGAPSALPVPSPSCPAEAPPAYSPQAAEGHYTVSYGTDSGEFSSVGEDFYRNRSQPPPLETLGLDADELILIPNGVQIFFVNPAGEVSAPSYPGYLRIVRFLDNSLDTVLNRPPGFLQVCDWLYPLVPDRSPVLKCTVGAYMFPDTMLQAAGCFVGVVLSSELPEDDRELFEDLLRQMSDLRLQTKDSSEEVNLSQIVPCEPSSEEKSKELPEWSEKVAHNILSGASWVSWGLVKGAEFTGKAIQKGASKLRERIQPEEKPVEVSPAVTRGLYIAKQATGGAAKVSQLLVDGVCTVANCVGKELAPHVKKHGSKLVPESLKRDKDGKSALDGAMVVAASSVQGFSTVWQGLECAAKCIVNNVSAETVQTVRYKYGHNAGEATHNAVDSAINVGLTAYNIDNIGIKAMVKKTAKQTGHTLLEDYQIVERPQRESQGGATSTEGRRDIGKQVEEEKPGAGKKDK

Target ORF information:

RefSeq Version	XM_030252562.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus spastic paraplegia 20, spartin (Troyer syndrome) homolog (human) (Spg20), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030252562.1

ORF Insert Sequence:

ATGGAGAGAG AACCAGAAAA TGGAGAACCT GCTGAAATTA AGATCATCAA GGAAGCATAC 
GAGAAGGCCT TTATGTTTGT TAATAAAGGA CTGAACACGG ACGAGTTAGG GCAGAAGGAA 
GAAGCAAAGA ACTATTACAA GCAAGGAATA GGACACCTGC TTAGGGGCAT CAGCATCGCG 
GCTGCAGAGC CGGGGCACAC AGGCCCTGCG TGGGAGGCTG CCAGGCAGAT GCAACAGAAA 
ATGAAGGAAA CGCTTCAGAA CGTCCGCACC AGGCTAGAGA TCCTGGAGAA GGGCCTTGCC 
ACTTCTCTAC GGAATGACCT TCAGGATGTG CCCAAGCTAT ACCCAGAGTT CCCGCCTAAA 
GACGCGTGCA AGAAGTCCCC AGAGCAGGAG TCCGTTAGTA CAGCGCCCCA GCGTGCTGAA 
GTCGATGGGA GCGCCTCAGC CGCGTGTGCC GGCCCAAGTG GTGCGCCTTC TGCTCTGCCC 
GTACCATCGC CCAGCTGCCC TGCCGAAGCG CCACCAGCCT ACAGCCCACA GGCTGCTGAG 
GGCCACTACA CTGTTTCCTA TGGAACAGAT TCCGGGGAGT TTTCATCCGT GGGAGAAGAC 
TTCTACAGGA ACCGTTCCCA GCCGCCCCCT CTTGAGACCT TGGGGCTCGA TGCAGATGAG 
TTGATCTTGA TACCAAACGG AGTGCAGATC TTTTTTGTAA ACCCTGCGGG AGAGGTCAGT 
GCTCCCTCGT ACCCTGGGTA TCTGCGGATC GTGCGGTTCT TGGATAATTC TCTGGATACA 
GTTCTGAACC GCCCTCCTGG GTTTCTCCAG GTGTGTGACT GGTTATACCC TCTAGTTCCT 
GATAGGTCAC CAGTTCTGAA GTGCACTGTG GGAGCCTACA TGTTTCCAGA TACCATGTTA 
CAAGCTGCTG GGTGCTTCGT AGGGGTTGTT CTTTCATCTG AGCTCCCAGA AGATGACAGA 
GAGCTGTTTG AGGATCTGTT GAGACAGATG TCTGACCTTC GGCTCCAGAC CAAAGATTCA 
AGTGAAGAAG TTAATCTGAG CCAGATTGTG CCCTGTGAGC CGAGTTCAGA AGAAAAATCG 
AAAGAATTGC CTGAATGGAG CGAGAAAGTG GCCCACAACA TCCTGTCAGG TGCTTCCTGG 
GTGAGCTGGG GTTTAGTCAA AGGTGCTGAA TTTACTGGCA AAGCAATTCA GAAAGGTGCT 
TCTAAACTCA GAGAGCGCAT TCAGCCGGAG GAAAAGCCAG TGGAGGTGAG CCCAGCTGTC 
ACCAGGGGTC TCTACATAGC GAAGCAGGCA ACCGGAGGAG CAGCCAAAGT CAGCCAGCTC 
CTGGTTGACG GCGTCTGCAC AGTAGCGAAT TGTGTTGGTA AAGAACTTGC TCCACACGTC 
AAGAAGCACG GAAGCAAACT GGTTCCGGAA TCCCTGAAGA GGGACAAAGA CGGGAAATCT 
GCCCTGGATG GCGCCATGGT CGTGGCAGCG AGTAGTGTTC AAGGATTCTC AACTGTCTGG 
CAAGGGTTGG AATGTGCAGC TAAATGCATC GTTAACAACG TGTCTGCAGA AACTGTACAG 
ACTGTGAGAT ACAAGTATGG GCACAACGCA GGAGAAGCTA CACATAACGC AGTGGATTCA 
GCTATCAATG TTGGGCTCAC TGCCTACAAT ATTGACAACA TCGGCATCAA AGCCATGGTG 
AAGAAAACTG CCAAGCAGAC GGGACACACT CTCCTCGAGG ACTACCAGAT CGTTGAGCGC 
CCTCAGAGGG AGAGTCAGGG AGGAGCCACA AGCACAGAAG GGAGAAGAGA CATAGGAAAG 
CAGGTGGAGG AAGAGAAGCC AGGGGCAGGA AAGAAGGACA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Methylation changes of SIRT1, KLF4, DAPK1 and SPG20 in B-lymphocytes derived from follicular and diffuse large B-cell lymphoma.
Leukemia research5789-96(2017 Jun)
Frazzi R,Zanetti E,Pistoni M,Tamagnini I,Valli R,Braglia L,Merli F

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Three cases of Troyer syndrome in two families of Filipino descent.
American journal of medical genetics. Part A170(7)1780-5(2016 Jul)
Butler S,Helbig KL,Alcaraz W,Seaver LH,Hsieh DT,Rohena L

The role of spartin and its novel ubiquitin binding region in DALIS occurrence.
Molecular biology of the cell25(8)1355-65(2014 Apr)
Karlsson AB,Washington J,Dimitrova V,Hooper C,Shekhtman A,Bakowska JC

Are alsin and spartin novel interaction partners?
Biochemical and biophysical research communications427(1)1-4(2012 Oct)
?obano?lu G,Ozansoy M,Ba?ak AN

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Spg20 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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