Ddx27 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ddx27
Entrez Gene ID	228889
Full Name	DEAD (Asp-Glu-Ala-Asp) box polypeptide 27
Synonyms	C86129
General protein information	Preferred Names DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 Names probable ATP-dependent RNA helicase DDX27 DEAD box protein 27 DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 27 RNA-helicase like protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 H3

mRNA and Protein(s)

mRNA	Protein	Name
NM_153065.3	NP_694705.2	probable ATP-dependent RNA helicase DDX27
XM_006499422.1	XP_006499485.1	probable ATP-dependent RNA helicase DDX27 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	DDX27	NP_060365.7
Pan troglodytes (chimpanzee)	DDX27	XP_001166506.1
Danio rerio (zebrafish)	ddx27	NP_001002869.1
	DDX27	XP_001099400.1
Canis lupus familiaris (dog)	DDX27	XP_534451.2
Mus musculus (house mouse)	Ddx27	NP_694705.2
Gallus gallus (chicken)	DDX27	NP_001006293.1
Rattus norvegicus (Norway rat)	Ddx27	NP_001129273.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011084	XP_309566.4
Xenopus tropicalis (tropical clawed frog)	ddx27	XP_004919149.1
Bos taurus (cattle)	DDX27	NP_001073740.1
Drosophila melanogaster (fruit fly)	Rs1	NP_651970.1
Caenorhabditis elegans (roundworm)	Y71G12B.8	NP_490891.2

Gene Ontology
Bibliography

Related articles in PubMed

An efficient method for generation of bi-allelic null mutant mouse embryonic stem cells and its application for investigating epigenetic modifiers.
Fisher CL, Marks H, Cho LT, Andrews R, Wormald S, Carroll T, Iyer V, Tate P, Rosen B, Stunnenberg HG, Fisher AG, Skarnes WC
Nucleic acids research45(21)e174(2017 Dec)

Tagging genes with cassette-exchange sites.
Cobellis G, Nicolaus G, Iovino M, Romito A, Marra E, Barbarisi M, Sardiello M, Di Giorgio FP, Iovino N, Zollo M, Ballabio A, Cortese R
Nucleic acids research33(4)e44(2005 Mar)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ddx27 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ddx27 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu02132	NM_153065.3 Latest version!	Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 (Ddx27), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu30419	XM_006499422.1 Latest version!	Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 (Ddx27), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ddx27 ( NM_153065.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_694705.2 Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 (Ddx27), mRNA.

Price & Availability↑

CloneID	OMu02132
Clone ID Related Accession (Same CDS sequence)	NM_153065.3
Accession Version	NM_153065.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	probable ATP-dependent RNA helicase DDX27
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK134274.1. On Apr 6, 2007 this sequence version replaced NM_153065.2. ##Evidence-Data-START## Transcript exon combination :: AK134274.1, AK146469.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGCTGGCGG AGCTCGGCTT CATCCGGACC ATCGGCGAGA ATGACGAGGT GCCGGTTGAG 
CCCGAGTCGG ACTCCGGAGA CGAGGAGGAG GAGGGACCCA TTGTGCTGGG CCGCAAGCAG 
AAAGCCTTGC AGAAGAACCG CAGTGCTGAC TTCAACCCTG ACTTTGTTTT CACTGAGAAG 
GAGGGCATGT ATGATGGCAG CTGGGCCCTG GCTGATGTCA TGAGCCAGCT CAAGAAGAAG 
CGGGCAGCCA CTACACTAGA TGAGAAGATT GAGAAAGTCC GGAAGAGAAG GAAGGCAGAG 
GACAAGGAAG CCAAGTCTGG GAAGGTGGAG GAGAAAGAAG GCCAGGCAGA CTCTGACCTG 
AAGGGGCAGG AGAATCCGGG TGAGGATGAG GCAGGCTCCA AGGATGAAGA CTCTGAGACT 
GACTACTCCT CTGAGGATGA GGAAATCCTC ACCAAAGCAG ACACACTCAA AGTGAAGGAG 
AAGAAGAAGA AGAAGAAGGG CCAGGCAGCA GGAGGATTCT TTGAAGATGC ATCCGAGTAT 
GACAAAAGCC TGTCCTTCCA GGACATGAAC CTCTCCCGGC CCCTCCTGAA GGCCATTACA 
GCCATGGGCT TCAAGCAGCC CACGCCAATC CAGAAGGCGT GTATCCCTGT GGGTCTGCTG 
GGGAAGGACA TCTGCGCCTG TGCAGCCACT GGGACAGGCA AAACTGCAGC CTTTGCACTG 
CCCGTCTTGG AGCGTCTGAT CTACAAGCCC CGCCAGGCTG CAGTCACTCG CGTGCTGGTG 
CTGGTCCCCA CGAGAGAGCT GGGCATCCAA GTGCACTCTG TCACCAAGCA GCTGGCCCAG 
TTCTGCAGCA TCACTACCTG CCTGGCTGTG GGTGGCCTGG ATGTGAAGTC TCAGGAAGCC 
GCTCTTCGGG CAGCACCAGA CATCCTCATT GCCACACCTG GCCGGCTCAT CGACCACCTC 
CATAACTGCC CCTCCTTCCA CCTGAGCAGC ATTGAAGTGC TCATCTTGGA TGAGGCTGAC 
AGGATGCTGG ATGAGTATTT CGAGGAGCAG ATGAAGGAGA TTATCCGAAT GTGTTCCCAC 
CACCGCCAGA CCATGCTCTT CTCGGCCACC ATGACAGATG AGGTGAAAGA TCTGGCTTCG 
GTCTCCTTAA AGAATCCTGT GCGGATATTT GTGAACAGCA ACACAGATGT CGCTCCCTTC 
CTGCGGCAAG AGTTCATACG GATCCGGCCT AATAGGGAAG GGGACCGGGA GGCCATTGTG 
GCAGCTCTGC TGATGAGAAC CTTCACTGAC CACGTGATGC TCTTCACCCA GACCAAGAAG 
CAGGCCCATC GCATGCACAT CCTCCTGGGG CTGCTGGGGC TGCAGGTGGG CGAGCTCCAC 
GGCAACTTGT CACAGACCCA GCGCCTGGAG GCCCTCCGGC GCTTTAAGGA CGAACAGATT 
GACATCCTGG TGGCCACAGA TGTGGCAGCC CGCGGTCTTG ACATTGAAGG AGTGAAAACA 
GTGATCAACT TCACCATGCC CAACACTGTT AAACACTATG TGCACCGGGT GGGGCGGACG 
GCCCGTGCTG GCAGGGCTGG ACGCTCTGTC TCCCTAGTGG GAGAGGAGGA GCGGAAGATG 
CTGAAGGAGA TCGTGAAGGC TGCCAAGGCG CCAGTGAAGG CTCGGATCCT TCCCCAGGAT 
GTTATCCTCA AGTTCCGGGA CAAGATTGAG AAATTGGAGA AAGATGTGTA TGCAGTGCTG 
CAGCTGGAGG CGGAGGAAAA AGAGATGCAG CAGTCGGAAG CCCAGATTGA CACAGCACAG 
CGACTCTTAG CCAAAGGGAA AGAGACAGCA GACCAGGAGC CTGAGAGGAG CTGGTTCCAG 
ACCAAAGAAG AGAGGAAGAA GGAGAAGATT GCTAAGGCTC TACAGGAGTT TGACTTGGCC 
TTAAGAGGAA AGAAGAAAAG GAAGAAGTTT ATGAAGGATG CCAAGAAAAA GGGGGAGATG 
ACGGCAGAGG AGAGGTCCCA GTTTGAAATC CTCAAGGCAC AGATGTTTGC AGAGCGGCTG 
GCCAAGAGGA ATCGCAGAAC CAAGCGAGCA CGGGCAATGC CTGAGGACGA GCCTACAGGC 
CCTGCTAAAA AGCAGAAGCA GCAGCAGAAG TCTGTGTTTG ATGAAGAACT CACCAACACA 
AGCAAGAAGG CCCTGAAACA GTATCGGGCT GGCCCTTCCT TTGAAGAGAG GAAACAGTCA 
GGTCTGCCCC GCCAGAGGAG AGGGAACTTC AAATCTAAGT CCAGGTACAA GAGAAAGAAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_694705.2
CDS	22..2304
Translation	MLAELGFIRTIGENDEVPVEPESDSGDEEEEGPIVLGRKQKALQKNRSADFNPDFVFTEKEGMYDGSWALADVMSQLKKKRAATTLDEKIEKVRKRRKAEDKEAKSGKVEEKEGQADSDLKGQENPGEDEAGSKDEDSETDYSSEDEEILTKADTLKVKEKKKKKKGQAAGGFFEDASEYDKSLSFQDMNLSRPLLKAITAMGFKQPTPIQKACIPVGLLGKDICACAATGTGKTAAFALPVLERLIYKPRQAAVTRVLVLVPTRELGIQVHSVTKQLAQFCSITTCLAVGGLDVKSQEAALRAAPDILIATPGRLIDHLHNCPSFHLSSIEVLILDEADRMLDEYFEEQMKEIIRMCSHHRQTMLFSATMTDEVKDLASVSLKNPVRIFVNSNTDVAPFLRQEFIRIRPNREGDREAIVAALLMRTFTDHVMLFTQTKKQAHRMHILLGLLGLQVGELHGNLSQTQRLEALRRFKDEQIDILVATDVAARGLDIEGVKTVINFTMPNTVKHYVHRVGRTARAGRAGRSVSLVGEEERKMLKEIVKAAKAPVKARILPQDVILKFRDKIEKLEKDVYAVLQLEAEEKEMQQSEAQIDTAQRLLAKGKETADQEPERSWFQTKEERKKEKIAKALQEFDLALRGKKKRKKFMKDAKKKGEMTAEERSQFEILKAQMFAERLAKRNRRTKRARAMPEDEPTGPAKKQKQQQKSVFDEELTNTSKKALKQYRAGPSFEERKQSGLPRQRRGNFKSKSRYKRKK

Target ORF information:

RefSeq Version	NM_153065.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 (Ddx27), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153065.3

ORF Insert Sequence:

ATGCTGGCGG AGCTCGGCTT CATCCGGACC ATCGGCGAGA ATGACGAGGT GCCGGTTGAG 
CCCGAGTCGG ACTCCGGAGA CGAGGAGGAG GAGGGACCCA TTGTGCTGGG CCGCAAGCAG 
AAAGCCTTGC AGAAGAACCG CAGTGCTGAC TTCAACCCTG ACTTTGTTTT CACTGAGAAG 
GAGGGCATGT ATGATGGCAG CTGGGCCCTG GCTGATGTCA TGAGCCAGCT CAAGAAGAAG 
CGGGCAGCCA CTACACTAGA TGAGAAGATT GAGAAAGTCC GGAAGAGAAG GAAGGCAGAG 
GACAAGGAAG CCAAGTCTGG GAAGGTGGAG GAGAAAGAAG GCCAGGCAGA CTCTGACCTG 
AAGGGGCAGG AGAATCCGGG TGAGGATGAG GCAGGCTCCA AGGATGAAGA CTCTGAGACT 
GACTACTCCT CTGAGGATGA GGAAATCCTC ACCAAAGCAG ACACACTCAA AGTGAAGGAG 
AAGAAGAAGA AGAAGAAGGG CCAGGCAGCA GGAGGATTCT TTGAAGATGC ATCCGAGTAT 
GACAAAAGCC TGTCCTTCCA GGACATGAAC CTCTCCCGGC CCCTCCTGAA GGCCATTACA 
GCCATGGGCT TCAAGCAGCC CACGCCAATC CAGAAGGCGT GTATCCCTGT GGGTCTGCTG 
GGGAAGGACA TCTGCGCCTG TGCAGCCACT GGGACAGGCA AAACTGCAGC CTTTGCACTG 
CCCGTCTTGG AGCGTCTGAT CTACAAGCCC CGCCAGGCTG CAGTCACTCG CGTGCTGGTG 
CTGGTCCCCA CGAGAGAGCT GGGCATCCAA GTGCACTCTG TCACCAAGCA GCTGGCCCAG 
TTCTGCAGCA TCACTACCTG CCTGGCTGTG GGTGGCCTGG ATGTGAAGTC TCAGGAAGCC 
GCTCTTCGGG CAGCACCAGA CATCCTCATT GCCACACCTG GCCGGCTCAT CGACCACCTC 
CATAACTGCC CCTCCTTCCA CCTGAGCAGC ATTGAAGTGC TCATCTTGGA TGAGGCTGAC 
AGGATGCTGG ATGAGTATTT CGAGGAGCAG ATGAAGGAGA TTATCCGAAT GTGTTCCCAC 
CACCGCCAGA CCATGCTCTT CTCGGCCACC ATGACAGATG AGGTGAAAGA TCTGGCTTCG 
GTCTCCTTAA AGAATCCTGT GCGGATATTT GTGAACAGCA ACACAGATGT CGCTCCCTTC 
CTGCGGCAAG AGTTCATACG GATCCGGCCT AATAGGGAAG GGGACCGGGA GGCCATTGTG 
GCAGCTCTGC TGATGAGAAC CTTCACTGAC CACGTGATGC TCTTCACCCA GACCAAGAAG 
CAGGCCCATC GCATGCACAT CCTCCTGGGG CTGCTGGGGC TGCAGGTGGG CGAGCTCCAC 
GGCAACTTGT CACAGACCCA GCGCCTGGAG GCCCTCCGGC GCTTTAAGGA CGAACAGATT 
GACATCCTGG TGGCCACAGA TGTGGCAGCC CGCGGTCTTG ACATTGAAGG AGTGAAAACA 
GTGATCAACT TCACCATGCC CAACACTGTT AAACACTATG TGCACCGGGT GGGGCGGACG 
GCCCGTGCTG GCAGGGCTGG ACGCTCTGTC TCCCTAGTGG GAGAGGAGGA GCGGAAGATG 
CTGAAGGAGA TCGTGAAGGC TGCCAAGGCG CCAGTGAAGG CTCGGATCCT TCCCCAGGAT 
GTTATCCTCA AGTTCCGGGA CAAGATTGAG AAATTGGAGA AAGATGTGTA TGCAGTGCTG 
CAGCTGGAGG CGGAGGAAAA AGAGATGCAG CAGTCGGAAG CCCAGATTGA CACAGCACAG 
CGACTCTTAG CCAAAGGGAA AGAGACAGCA GACCAGGAGC CTGAGAGGAG CTGGTTCCAG 
ACCAAAGAAG AGAGGAAGAA GGAGAAGATT GCTAAGGCTC TACAGGAGTT TGACTTGGCC 
TTAAGAGGAA AGAAGAAAAG GAAGAAGTTT ATGAAGGATG CCAAGAAAAA GGGGGAGATG 
ACGGCAGAGG AGAGGTCCCA GTTTGAAATC CTCAAGGCAC AGATGTTTGC AGAGCGGCTG 
GCCAAGAGGA ATCGCAGAAC CAAGCGAGCA CGGGCAATGC CTGAGGACGA GCCTACAGGC 
CCTGCTAAAA AGCAGAAGCA GCAGCAGAAG TCTGTGTTTG ATGAAGAACT CACCAACACA 
AGCAAGAAGG CCCTGAAACA GTATCGGGCT GGCCCTTCCT TTGAAGAGAG GAAACAGTCA 
GGTCTGCCCC GCCAGAGGAG AGGGAACTTC AAATCTAAGT CCAGGTACAA GAGAAAGAAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ddx27 ( XM_006499422.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006499485.1 Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 (Ddx27), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu30419
Clone ID Related Accession (Same CDS sequence)	XM_006499422.1
Accession Version	XM_006499422.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2367bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	probable ATP-dependent RNA helicase DDX27 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGCGG AGCTCGGCTT CATCCGGACC ATCGGCGAGA ATGACGAGGT GCCGGTTGAG 
CCCGAGTCGG ACTCCGGAGA CGAGGAGGAG GAGGGACCCA TTGTGCTGGG CCGCAAGCAG 
AAAGCCTTGC AGAAGAACCG CAGTGCTGAC TTCAACCCTG ACTTTGTTTT CACTGAGAAG 
GAGGGCATGT ATGATGGCAG CTGGGCCCTG GCTGATGTCA TGAGCCAGCT CAAGAAGAAG 
CGGGCAGCCA CTACACTAGA TGAGAAGATT GAGAAAGTCC GGAAGAGAAG GAAGGCAGAG 
GACAAGGAAG CCAAGTCTGG GAAGGTGGAG GAGAAAGAAG GCCAGGCAGA CTCTGACCTG 
AAGGGGCAGG AGAATCCGGG TGAGGATGAG GCAGGCTCCA AGGATGAAGA CTCTGAGACT 
GACTACTCCT CTGAGGATGA GGAAATCCTC ACCAAAGCAG ACACACTCAA AGTGAAGGAG 
AAGAAGAAGA AGAAGAAGGG CCAGGTGAGC CTGGGTCTGA GGTTGCTGAA AGGACATGCA 
GGCTTGCTTG CTTTGCTGAG TTCTTTCCTG TCGTCTGATC TGTTGCAGGC AGCAGGAGGA 
TTCTTTGAAG ATGCATCCGA GTATGACAAA AGCCTGTCCT TCCAGGACAT GAACCTCTCC 
CGGCCCCTCC TGAAGGCCAT TACAGCCATG GGCTTCAAGC AGCCCACGCC AATCCAGAAG 
GCGTGTATCC CTGTGGGTCT GCTGGGGAAG GACATCTGCG CCTGTGCAGC CACTGGGACA 
GGCAAAACTG CAGCCTTTGC ACTGCCCGTC TTGGAGCGTC TGATCTACAA GCCCCGCCAG 
GCTGCAGTCA CTCGCGTGCT GGTGCTGGTC CCCACGAGAG AGCTGGGCAT CCAAGTGCAC 
TCTGTCACCA AGCAGCTGGC CCAGTTCTGC AGCATCACTA CCTGCCTGGC TGTGGGTGGC 
CTGGATGTGA AGTCTCAGGA AGCCGCTCTT CGGGCAGCAC CAGACATCCT CATTGCCACA 
CCTGGCCGGC TCATCGACCA CCTCCATAAC TGCCCCTCCT TCCACCTGAG CAGCATTGAA 
GTGCTCATCT TGGATGAGGC TGACAGGATG CTGGATGAGT ATTTCGAGGA GCAGATGAAG 
GAGATTATCC GAATGTGTTC CCACCACCGC CAGACCATGC TCTTCTCGGC CACCATGACA 
GATGAGGTGA AAGATCTGGC TTCGGTCTCC TTAAAGAATC CTGTGCGGAT ATTTGTGAAC 
AGCAACACAG ATGTCGCTCC CTTCCTGCGG CAAGAGTTCA TACGGATCCG GCCTAATAGG 
GAAGGGGACC GGGAGGCCAT TGTGGCAGCT CTGCTGATGA GAACCTTCAC TGACCACGTG 
ATGCTCTTCA CCCAGACCAA GAAGCAGGCC CATCGCATGC ACATCCTCCT GGGGCTGCTG 
GGGCTGCAGG TGGGCGAGCT CCACGGCAAC TTGTCACAGA CCCAGCGCCT GGAGGCCCTC 
CGGCGCTTTA AGGACGAACA GATTGACATC CTGGTGGCCA CAGATGTGGC AGCCCGCGGT 
CTTGACATTG AAGGAGTGAA AACAGTGATC AACTTCACCA TGCCCAACAC TGTTAAACAC 
TATGTGCACC GGGTGGGGCG GACGGCCCGT GCTGGCAGGG CTGGACGCTC TGTCTCCCTA 
GTGGGAGAGG AGGAGCGGAA GATGCTGAAG GAGATCGTGA AGGCTGCCAA GGCGCCAGTG 
AAGGCTCGGA TCCTTCCCCA GGATGTTATC CTCAAGTTCC GGGACAAGAT TGAGAAATTG 
GAGAAAGATG TGTATGCAGT GCTGCAGCTG GAGGCGGAGG AAAAAGAGAT GCAGCAGTCG 
GAAGCCCAGA TTGACACAGC ACAGCGACTC TTAGCCAAAG GGAAAGAGAC AGCAGACCAG 
GAGCCTGAGA GGAGCTGGTT CCAGACCAAA GAAGAGAGGA AGAAGGAGAA GATTGCTAAG 
GCTCTACAGG AGTTTGACTT GGCCTTAAGA GGAAAGAAGA AAAGGAAGAA GTTTATGAAG 
GATGCCAAGA AAAAGGGGGA GATGACGGCA GAGGAGAGGT CCCAGTTTGA AATCCTCAAG 
GCACAGATGT TTGCAGAGCG GCTGGCCAAG AGGAATCGCA GAACCAAGCG AGCACGGGCA 
ATGCCTGAGG ACGAGCCTAC AGGCCCTGCT AAAAAGCAGA AGCAGCAGCA GAAGTCTGTG 
TTTGATGAAG AACTCACCAA CACAAGCAAG AAGGCCCTGA AACAGTATCG GGCTGGCCCT 
TCCTTTGAAG AGAGGAAACA GTCAGGTCTG CCCCGCCAGA GGAGAGGGAA CTTCAAATCT 
AAGTCCAGGT ACAAGAGAAA GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006499485.1
CDS	80..2446
Translation	MLAELGFIRTIGENDEVPVEPESDSGDEEEEGPIVLGRKQKALQKNRSADFNPDFVFTEKEGMYDGSWALADVMSQLKKKRAATTLDEKIEKVRKRRKAEDKEAKSGKVEEKEGQADSDLKGQENPGEDEAGSKDEDSETDYSSEDEEILTKADTLKVKEKKKKKKGQVSLGLRLLKGHAGLLALLSSFLSSDLLQAAGGFFEDASEYDKSLSFQDMNLSRPLLKAITAMGFKQPTPIQKACIPVGLLGKDICACAATGTGKTAAFALPVLERLIYKPRQAAVTRVLVLVPTRELGIQVHSVTKQLAQFCSITTCLAVGGLDVKSQEAALRAAPDILIATPGRLIDHLHNCPSFHLSSIEVLILDEADRMLDEYFEEQMKEIIRMCSHHRQTMLFSATMTDEVKDLASVSLKNPVRIFVNSNTDVAPFLRQEFIRIRPNREGDREAIVAALLMRTFTDHVMLFTQTKKQAHRMHILLGLLGLQVGELHGNLSQTQRLEALRRFKDEQIDILVATDVAARGLDIEGVKTVINFTMPNTVKHYVHRVGRTARAGRAGRSVSLVGEEERKMLKEIVKAAKAPVKARILPQDVILKFRDKIEKLEKDVYAVLQLEAEEKEMQQSEAQIDTAQRLLAKGKETADQEPERSWFQTKEERKKEKIAKALQEFDLALRGKKKRKKFMKDAKKKGEMTAEERSQFEILKAQMFAERLAKRNRRTKRARAMPEDEPTGPAKKQKQQQKSVFDEELTNTSKKALKQYRAGPSFEERKQSGLPRQRRGNFKSKSRYKRKK

Target ORF information:

RefSeq Version	XM_006499422.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 (Ddx27), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499422.1

ORF Insert Sequence:

ATGCTGGCGG AGCTCGGCTT CATCCGGACC ATCGGCGAGA ATGACGAGGT GCCGGTTGAG 
CCCGAGTCGG ACTCCGGAGA CGAGGAGGAG GAGGGACCCA TTGTGCTGGG CCGCAAGCAG 
AAAGCCTTGC AGAAGAACCG CAGTGCTGAC TTCAACCCTG ACTTTGTTTT CACTGAGAAG 
GAGGGCATGT ATGATGGCAG CTGGGCCCTG GCTGATGTCA TGAGCCAGCT CAAGAAGAAG 
CGGGCAGCCA CTACACTAGA TGAGAAGATT GAGAAAGTCC GGAAGAGAAG GAAGGCAGAG 
GACAAGGAAG CCAAGTCTGG GAAGGTGGAG GAGAAAGAAG GCCAGGCAGA CTCTGACCTG 
AAGGGGCAGG AGAATCCGGG TGAGGATGAG GCAGGCTCCA AGGATGAAGA CTCTGAGACT 
GACTACTCCT CTGAGGATGA GGAAATCCTC ACCAAAGCAG ACACACTCAA AGTGAAGGAG 
AAGAAGAAGA AGAAGAAGGG CCAGGTGAGC CTGGGTCTGA GGTTGCTGAA AGGACATGCA 
GGCTTGCTTG CTTTGCTGAG TTCTTTCCTG TCGTCTGATC TGTTGCAGGC AGCAGGAGGA 
TTCTTTGAAG ATGCATCCGA GTATGACAAA AGCCTGTCCT TCCAGGACAT GAACCTCTCC 
CGGCCCCTCC TGAAGGCCAT TACAGCCATG GGCTTCAAGC AGCCCACGCC AATCCAGAAG 
GCGTGTATCC CTGTGGGTCT GCTGGGGAAG GACATCTGCG CCTGTGCAGC CACTGGGACA 
GGCAAAACTG CAGCCTTTGC ACTGCCCGTC TTGGAGCGTC TGATCTACAA GCCCCGCCAG 
GCTGCAGTCA CTCGCGTGCT GGTGCTGGTC CCCACGAGAG AGCTGGGCAT CCAAGTGCAC 
TCTGTCACCA AGCAGCTGGC CCAGTTCTGC AGCATCACTA CCTGCCTGGC TGTGGGTGGC 
CTGGATGTGA AGTCTCAGGA AGCCGCTCTT CGGGCAGCAC CAGACATCCT CATTGCCACA 
CCTGGCCGGC TCATCGACCA CCTCCATAAC TGCCCCTCCT TCCACCTGAG CAGCATTGAA 
GTGCTCATCT TGGATGAGGC TGACAGGATG CTGGATGAGT ATTTCGAGGA GCAGATGAAG 
GAGATTATCC GAATGTGTTC CCACCACCGC CAGACCATGC TCTTCTCGGC CACCATGACA 
GATGAGGTGA AAGATCTGGC TTCGGTCTCC TTAAAGAATC CTGTGCGGAT ATTTGTGAAC 
AGCAACACAG ATGTCGCTCC CTTCCTGCGG CAAGAGTTCA TACGGATCCG GCCTAATAGG 
GAAGGGGACC GGGAGGCCAT TGTGGCAGCT CTGCTGATGA GAACCTTCAC TGACCACGTG 
ATGCTCTTCA CCCAGACCAA GAAGCAGGCC CATCGCATGC ACATCCTCCT GGGGCTGCTG 
GGGCTGCAGG TGGGCGAGCT CCACGGCAAC TTGTCACAGA CCCAGCGCCT GGAGGCCCTC 
CGGCGCTTTA AGGACGAACA GATTGACATC CTGGTGGCCA CAGATGTGGC AGCCCGCGGT 
CTTGACATTG AAGGAGTGAA AACAGTGATC AACTTCACCA TGCCCAACAC TGTTAAACAC 
TATGTGCACC GGGTGGGGCG GACGGCCCGT GCTGGCAGGG CTGGACGCTC TGTCTCCCTA 
GTGGGAGAGG AGGAGCGGAA GATGCTGAAG GAGATCGTGA AGGCTGCCAA GGCGCCAGTG 
AAGGCTCGGA TCCTTCCCCA GGATGTTATC CTCAAGTTCC GGGACAAGAT TGAGAAATTG 
GAGAAAGATG TGTATGCAGT GCTGCAGCTG GAGGCGGAGG AAAAAGAGAT GCAGCAGTCG 
GAAGCCCAGA TTGACACAGC ACAGCGACTC TTAGCCAAAG GGAAAGAGAC AGCAGACCAG 
GAGCCTGAGA GGAGCTGGTT CCAGACCAAA GAAGAGAGGA AGAAGGAGAA GATTGCTAAG 
GCTCTACAGG AGTTTGACTT GGCCTTAAGA GGAAAGAAGA AAAGGAAGAA GTTTATGAAG 
GATGCCAAGA AAAAGGGGGA GATGACGGCA GAGGAGAGGT CCCAGTTTGA AATCCTCAAG 
GCACAGATGT TTGCAGAGCG GCTGGCCAAG AGGAATCGCA GAACCAAGCG AGCACGGGCA 
ATGCCTGAGG ACGAGCCTAC AGGCCCTGCT AAAAAGCAGA AGCAGCAGCA GAAGTCTGTG 
TTTGATGAAG AACTCACCAA CACAAGCAAG AAGGCCCTGA AACAGTATCG GGCTGGCCCT 
TCCTTTGAAG AGAGGAAACA GTCAGGTCTG CCCCGCCAGA GGAGAGGGAA CTTCAAATCT 
AAGTCCAGGT ACAAGAGAAA GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An efficient method for generation of bi-allelic null mutant mouse embryonic stem cells and its application for investigating epigenetic modifiers.
Nucleic acids research45(21)e174(2017 Dec)
Fisher CL,Marks H,Cho LT,Andrews R,Wormald S,Carroll T,Iyer V,Tate P,Rosen B,Stunnenberg HG,Fisher AG,Skarnes WC

Tagging genes with cassette-exchange sites.
Nucleic acids research33(4)e44(2005 Mar)
Cobellis G,Nicolaus G,Iovino M,Romito A,Marra E,Barbarisi M,Sardiello M,Di Giorgio FP,Iovino N,Zollo M,Ballabio A,Cortese R

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

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Ddx27 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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