Qrfp cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Qrfp
Entrez Gene ID	227717
Full Name	pyroglutamylated RFamide peptide
Synonyms	P518
General protein information	Preferred Names pyroglutamylated RFamide peptide Names orexigenic neuropeptide QRFP P518 precursor protein (P518)
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 B
Summary	This gene encodes a preproprotein that is proteolytically processed to generate multiple protein products. The encoded products are members of the RFamide family of neuropeptides, characterized by their common protein C-terminus consisting of an arginine (R) and an amidated phenylalanine (F). These products include the neuropeptides QRFP-26 (26RFa) and the N-terminally extended form, QRFP-43 (43RFa). Both of these neuropeptides bind to the pyroglutamylated RFamide peptide receptor (QRFPR) and may regulate blood pressure, reproduction and food intake. [provided by RefSeq, Sep 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_183424.4	NP_906269.1	orexigenic neuropeptide QRFP preproprotein

Pathways from BioSystems

Homologies

	QRFP	XP_001106500.1
Canis lupus familiaris (dog)	QRFP	XP_850751.2
Bos taurus (cattle)	QRFP	NP_937865.1
Rattus norvegicus (Norway rat)	LOC100909783	XP_003749488.1
Pan troglodytes (chimpanzee)	QRFP	XP_001166241.3
Mus musculus (house mouse)	Qrfp	NP_906269.1
Homo sapiens (human)	QRFP	NP_937823.1
Rattus norvegicus (Norway rat)	Qrfp	NP_937843.1

Gene Ontology
Bibliography

Related articles in PubMed

QRFP-Deficient Mice Are Hypophagic, Lean, Hypoactive and Exhibit Increased Anxiety-Like Behavior.
Okamoto K, Yamasaki M, Takao K, Soya S, Iwasaki M, Sasaki K, Magoori K, Sakakibara I, Miyakawa T, Mieda M, Watanabe M, Sakai J, Yanagisawa M, Sakurai T
PloS one11(11)e0164716(2016)

Hypothalamic Neuropeptide 26RFa Acts as an Incretin to Regulate Glucose Homeostasis.
Pr?vost G, Jeandel L, Arabo A, Co?ffier M, El Ouahli M, Picot M, Alexandre D, Gobet F, Leprince J, Berrahmoune H, D?chelotte P, Malagon M, Bonner C, Kerr-Conte J, Chigr F, Lefebvre H, Anouar Y, Chartrel N
Diabetes64(8)2805-16(2015 Aug)

QRFP-43 inhibits lipolysis by preventing ligand-induced complex formation between perilipin A, caveolin-1, the catalytic subunit of protein kinase and hormone-sensitive lipase in 3T3-L1 adipocytes.
Mulumba M, Granata R, Marleau S, Ong H
Biochimica et biophysica acta1851(5)657-66(2015 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

QRFP is an important downstream mediator of the arcuate nucleus and regulates feeding behavior, mood, wakefulness and activity.
Title: QRFP-Deficient Mice Are Hypophagic, Lean, Hypoactive and Exhibit Increased Anxiety-Like Behavior.

26RFa attenuates the hyperglycemia induced by a glucose load, potentiates insulin sensitivity, and increases plasma insulin concentrations.
Title: Hypothalamic Neuropeptide 26RFa Acts as an Incretin to Regulate Glucose Homeostasis.

Macrophage-conditioned medium containing increased amounts of QRFP preserves adipogenesis in adipocytes.
Title: Pyroglutamylated RF-amide peptide (QRFP) gene is regulated by metabolic endotoxemia.

The present review discusses the role of QRFP in the regulation of feeding behavior, with emphasis on the intake of dietary fat.
Title: Hypothalamic QRFP: regulation of food intake and fat selection.

Results suggest that GPR103b and QRFP work in an autocrine/paracrine manner to regulate adipogenesis.
Title: GPR103b functions in the peripheral regulation of adipogenesis.

The following Qrfp gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Qrfp cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu18952	NM_183424.4 Latest version!	Mus musculus pyroglutamylated RFamide peptide (Qrfp), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Qrfp ( NM_183424.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_906269.1 Mus musculus pyroglutamylated RFamide peptide (Qrfp), mRNA.

Price & Availability↑

CloneID	OMu18952
Clone ID Related Accession (Same CDS sequence)	NM_183424.4
Accession Version	NM_183424.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 375bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	orexigenic neuropeptide QRFP preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BB634210.1 and AK029144.1. On Sep 26, 2015 this sequence version replaced NM_183424.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK029144.1, BB613620.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849378 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGGGGCT TCCGGCCTTT GCTTTCCCTA CTTCTCCCTC TGAGTGCCTG CTTTCCCCTG 
CTGGACAGAA GGGGACCCAC AGACATCGGT GACATCGGAG CCAGGATGAA CTGGGCCCAG 
CTGGCTGAGG GACATCCCCC CAACTCGGTT CAAAATCCAC AGCCACAGGC CCTGCTTGTG 
GTGGCCAGGG AGCAGCAGGC CTCCCACAGG GAGCACACCG GCTTCCGTCT AGGGAGGCAA 
GACGGTAGCA GTGAGGCCGC AGGGTTCCTG CCCGCCGACT CGGAGAAGGC CAGCGGCCCT 
CTGGGGACTC TGGCAGAGGA GCTGAGCAGC TACAGCCGGA GGAAGGGAGG CTTCAGCTTC 
CGCTTTGGAC GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_906269.1
CDS	175..549
Translation	MRGFRPLLSLLLPLSACFPLLDRRGPTDIGDIGARMNWAQLAEGHPPNSVQNPQPQALLVVAREQQASHREHTGFRLGRQDGSSEAAGFLPADSEKASGPLGTLAEELSSYSRRKGGFSFRFGR

Target ORF information:

RefSeq Version	NM_183424.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus pyroglutamylated RFamide peptide (Qrfp), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_183424.4

ORF Insert Sequence:

ATGAGGGGCT TCCGGCCTTT GCTTTCCCTA CTTCTCCCTC TGAGTGCCTG CTTTCCCCTG 
CTGGACAGAA GGGGACCCAC AGACATCGGT GACATCGGAG CCAGGATGAA CTGGGCCCAG 
CTGGCTGAGG GACATCCCCC CAACTCGGTT CAAAATCCAC AGCCACAGGC CCTGCTTGTG 
GTGGCCAGGG AGCAGCAGGC CTCCCACAGG GAGCACACCG GCTTCCGTCT AGGGAGGCAA 
GACGGTAGCA GTGAGGCCGC AGGGTTCCTG CCCGCCGACT CGGAGAAGGC CAGCGGCCCT 
CTGGGGACTC TGGCAGAGGA GCTGAGCAGC TACAGCCGGA GGAAGGGAGG CTTCAGCTTC 
CGCTTTGGAC GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

QRFP-Deficient Mice Are Hypophagic, Lean, Hypoactive and Exhibit Increased Anxiety-Like Behavior.
PloS one11(11)e0164716(2016)
Okamoto K,Yamasaki M,Takao K,Soya S,Iwasaki M,Sasaki K,Magoori K,Sakakibara I,Miyakawa T,Mieda M,Watanabe M,Sakai J,Yanagisawa M,Sakurai T

Hypothalamic Neuropeptide 26RFa Acts as an Incretin to Regulate Glucose Homeostasis.
Diabetes64(8)2805-16(2015 Aug)
Pr?vost G,Jeandel L,Arabo A,Co?ffier M,El Ouahli M,Picot M,Alexandre D,Gobet F,Leprince J,Berrahmoune H,D?chelotte P,Malagon M,Bonner C,Kerr-Conte J,Chigr F,Lefebvre H,Anouar Y,Chartrel N

QRFP-43 inhibits lipolysis by preventing ligand-induced complex formation between perilipin A, caveolin-1, the catalytic subunit of protein kinase and hormone-sensitive lipase in 3T3-L1 adipocytes.
Biochimica et biophysica acta1851(5)657-66(2015 May)
Mulumba M,Granata R,Marleau S,Ong H

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Qrfp cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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