Cps1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cps1
Entrez Gene ID	227231
Full Name	carbamoyl-phosphate synthetase 1
Synonyms	4732433M03Rik,CPS,D1Ucla3
General protein information	Preferred Names carbamoyl-phosphate synthetase 1 Names carbamoyl-phosphate synthase [ammonia], mitochondrial CPSase I carbamoyl-phosphate synthetase I carbamoylphosphate synthetase I
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1 C3\|1 33.75 cM
Summary	This gene encodes a protein localized to the inner mitochondrial matrix. The encoded protein plays a role in the detoxification of ammonia by catalyzing the first step in the urea cycle in which carbomyl-phosphate is synthesized from ammonia and bicarbonate. Carbamoyl-phosphate is subsequently converted to urea that is excreted by the kidneys. Deficiency of the encoded enzyme leads to an accumulation of ammonia in the blood. High levels of ammonia are toxic to the central nervous system and result in neurological disorders. [provided by RefSeq, Oct 2013].

mRNA and Protein(s)

mRNA	Protein	Name
XM_011238500.1	XP_011236802.1	carbamoyl-phosphate synthase [ammonia], mitochondrial isoform X1
NM_001080809.2	NP_001074278.1	carbamoyl-phosphate synthase [ammonia], mitochondrial precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	CPS1	NP_001179187.1
Xenopus tropicalis (tropical clawed frog)	cps1	XP_004917905.1
Homo sapiens (human)	CPS1	NP_001116105.1
Rattus norvegicus (Norway rat)	Cps1	NP_058768.1
Pan troglodytes (chimpanzee)	CPS1	XP_001146604.2
Canis lupus familiaris (dog)	CPS1	XP_545627.2
Mus musculus (house mouse)	Cps1	NP_001074278.1
Danio rerio (zebrafish)	cps1	XP_683282.4
	CPS1	XP_001110375.1
Gallus gallus (chicken)	CPS1	NP_001039306.1
Arabidopsis thaliana (thale cress)	CARB	NP_564338.1
Oryza sativa (rice)	Os01g0570700	NP_001043373.1

Gene Ontology
Bibliography

Related articles in PubMed

The RSPO-LGR4/5-ZNRF3/RNF43 module controls liver zonation and size.
Planas-Paz L, Orsini V, Boulter L, Calabrese D, Pikiolek M, Nigsch F, Xie Y, Roma G, Donovan A, Marti P, Beckmann N, Dill MT, Carbone W, Bergling S, Isken A, Mueller M, Kinzel B, Yang Y, Mao X, Nicholson TB, Zamponi R, Capodieci P, Valdez R, Rivera D, Loew A, Ukomadu C, Terracciano LM, Bouwmeester T, Cong F, Heim MH, Forbes SJ, Ruffner H, Tchorz JS
Nature cell biology18(5)467-79(2016 05)

Overexpression of SIRT5 confirms its involvement in deacetylation and activation of carbamoyl phosphate synthetase 1.
Ogura M, Nakamura Y, Tanaka D, Zhuang X, Fujita Y, Obara A, Hamasaki A, Hosokawa M, Inagaki N
Biochemical and biophysical research communications393(1)73-8(2010 Feb)

SIRT5 Deacetylates carbamoyl phosphate synthetase 1 and regulates the urea cycle.
Nakagawa T, Lomb DJ, Haigis MC, Guarente L
Cell137(3)560-70(2009 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

these data suggest that AhR activation promotes CPS1 recruitment to DNA enhancer sites in the genome, resulting in a specific enzyme-independent post-translational modification of the linker histone H1 protein (H1K34hcit), pivotal in altering local chromatin structure and transcriptional activation.
Title: Homocitrullination Is a Novel Histone H1 Epigenetic Mark Dependent on Aryl Hydrocarbon Receptor Recruitment of Carbamoyl Phosphate Synthase 1.

The results suggest that elevated isoaspartate and CPS-1, and reduced CA-III levels could serve as biomarkers of hepatocellular injury.
Title: Isoaspartate, carbamoyl phosphate synthase-1, and carbonic anhydrase-III as biomarkers of liver injury.

CPS1 becomes readily detectable upon hepatocyte apoptotic and necrotic death. Its abundance and short serum half-life suggest that it may be a useful prognostic biomarker in acute liver injury.
Title: Carbamoyl phosphate synthetase-1 is a rapid turnover biomarker in mouse and human acute liver injury.

Taken together with previous findings regarding CPS1 structure and function, homology modeling of mouse CPS1 suggested that nitration at Y1450 in an alpha-helix of allosteric domain prevents activation of CPS1 by its activator, N-acetyl-l-glutamate.
Title: Protein tyrosine nitration of mitochondrial carbamoyl phosphate synthetase 1 and its functional consequences.

Because ammonia generated during fasting is toxic, SIRT5 protein might play a protective role by converting ammonia to non-toxic urea through deacetylation and activation of CPS1.
Title: Overexpression of SIRT5 confirms its involvement in deacetylation and activation of carbamoyl phosphate synthetase 1.

The following Cps1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cps1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu16468	NM_001080809.2 Latest version!	Mus musculus carbamoyl-phosphate synthetase 1 (Cps1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu16468	XM_011238500.1 Latest version!	Mus musculus carbamoyl-phosphate synthetase 1 (Cps1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cps1 ( NM_001080809.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001074278.1 Mus musculus carbamoyl-phosphate synthetase 1 (Cps1), mRNA.

Price & Availability↑

CloneID	OMu16468
Clone ID Related Accession (Same CDS sequence)	XM_011238500.1 , NM_001080809.2
Accession Version	NM_001080809.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4503bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	carbamoyl-phosphate synthase [ammonia], mitochondrial precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY790286.1, BC126969.1, AK028683.1 and AI046442.1. On Nov 2, 2013 this sequence version replaced NM_001080809.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC126969.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849377, SAMN00849378 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGACGAGGA TTTTGACAGC TTGCAAAGTG GTGAAGACCC TGAAGAGTGG CTTTGGTTTT 
GCCAATGTGA CTACGAAGCG ACAGTGGGAC TTCTCCAGAC CTGGCATCAG GCTCCTTTCT 
GTGAAGGCAA AGACAGCACA CATTGTTCTG GAAGATGGAA CTAAGATGAA AGGCTACTCC 
TTTGGCCATC CCTCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCTT AGGAGGGTAC 
CCGGAAGCAC TTACTGATCC CGCCTACAAG GGGCAGATCC TCACCATGGC CAACCCTATC 
ATTGGGAACG GTGGGGCTCC CGACACCACT GCCCGAGACG AACTGGGACT GAATAAGTAC 
ATGGAGTCTG ATGGGATCAA GGTGGCAGGT CTGCTGGTTC TGAATTACAG TAATGACTAT 
AACCACTGGC TGGCTACCAA GAGTCTGGGT CAATGGCTGC AGGAGGAAAA GGTCCCTGCA 
ATTTATGGAG TGGATACAAG AATGCTGACT AAAATAATTC GGGATAAGGG TACCATGCTT 
GGGAAGATTG AATTTGAAGG CCAGTCTGTG GATTTTGTGG ATCCCAATAA GCAGAATTTG 
ATTGCTGAGG TTTCAACCAA GGATGTCAAG GTGTTTGGCA AAGGAAACCC CACGAAAGTG 
GTAGCTGTGG ACTGTGGGAT AAAAAACAAT GTCATCCGCC TGCTAGTTAA GCGAGGAGCT 
GAAGTGCATT TGGTTCCCTG GAATCACGAC TTCACCCAGA TGGAGTATGA TGGACTTTTG 
ATTGCTGGGG GACCTGGGAA TCCAGCTCTT GCACAGCCAC TAATTCAGAA TGTGAAAAAG 
ATTTTGGAGA GTGACCGCAA AGAGCCATTG TTTGGAATCA GTACAGGAAA CATAATAACA 
GGATTGGCTG CTGGTGCCAA ATCCTACAAG ATGTCCATGG CCAACAGAGG ACAGAACCAA 
CCTGTTTTGA ATATCACAAA CAGACAGGCT TTCATAACTG CTCAGAATCA TGGCTATGCT 
CTGGACAACA CACTCCCTGC TGGCTGGAAA CCACTGTTTG TGAATGTCAA TGATCAAACA 
AACGAGGGGA TTATGCATGA GAGCAAACCT TTCTTCGCTG TGCAGTTCCA CCCAGAGGTC 
AGCCCGGGGC CAACAGACAC TGAGTACCTA TTTGATTCCT TCTTCTCCCT GATAAAGAAG 
GGCAAAGGCA CCACCATTAC CTCAGTTCTG CCCAAGCCAG CGTTGGTGGC ATCTCGTGTC 
GAGGTTTCCA AGGTCCTCAT CCTGGGATCA GGAGGTCTGT CCATTGGTCA GGCAGGTGAA 
TTTGATTACT CGGGATCTCA AGCTGTGAAA GCCATGAAGG AAGAAAATGT CAAAACAGTC 
CTGATGAACC CGAACATCGC ATCCGTGCAG ACCAACGAGG TGGGATTGAA GCAGGCAGAT 
GCAGTCTACT TTCTCCCTAT TACCCCCCAG TTTGTCACAG AAGTTATCAA GGCTGAACGG 
CCTGATGGGC TGATTCTGGG CATGGGTGGC CAGACAGCTC TGAACTGTGG AGTGGAACTA 
TTCAAGAGGG GTGTGCTCAA GGAATATGGG GTGAAGGTCT TGGGCACATC AGTTGAGTCC 
ATTATGGCCA CAGAAGACAG GCAGCTGTTC TCAGACAAGC TGAATGAGAT CAACGAGAAG 
ATCGCACCCA GTTTTGCAGT GGAATCAATG GAGGATGCCT TGAAGGCAGC AGACACCATT 
GGCTACCCTG TGATGATTCG GTCTGCATAT GCTCTGGGTG GCTTAGGCTC TGGCATCTGT 
CCCAATAAGG AGACCTTAAT AGACCTTGGC ACAAAAGCAT TTGCTATGAC CAACCAGATC 
CTGGTGGAGC GGTCAGTGAC AGGTTGGAAA GAAATAGAAT ATGAAGTTGT CCGAGATGCA 
GATGATAACT GTGTCACAGT CTGTAACATG GAAAATGTTG ACGCCATGGG TGTTCACACA 
GGTGACTCGG TTGTCGTGGC CCCAGCCCAG ACACTCTCCA ATGCAGAGTT CCAGATGTTG 
AGACGCACTT CAGTCAATGT TGTTCGTCAC TTGGGCATTG TGGGCGAATG CAACATTCAG 
TTTGCTCTTC ACCCCACTTC CATGGAATAC TGTATCATTG AAGTGAATGC CAGGCTGTCC 
CGGAGTTCTG CCCTGGCCTC CAAGGCCACT GGCTACCCAC TGGCATTCAT TGCAGCAAAG 
ATTGCTCTAG GAATCCCACT TCCAGAAATC AAGAATGTTG TATCTGGGAA GACCTCAGCC 
TGCTTCGAAC CTAGCCTGGA TTACATGGTG ACCAAGATTC CTCGCTGGGA TCTTGACCGT 
TTCCATGGAA CATCCAGCCG AATTGGTAGC TCAATGAAAA GTGTGGGCGA GGTCATGGCC 
ATTGGTCGCA CCTTTGAGGA AAGTTTCCAG AAGGCTCTGA GGATGTGCCA TCCATCTGTG 
GATGGGTTCA CTCCCCGGCT CCCAATGAAT AAGGAATGGC CAGCAAACCT GGATCTGAAG 
AAAGAGCTGT CTGAGCCCTC CAGCACCCGC ATCTACGCCA TTGCTAAGGC CTTGGAGAAC 
AACATGTCCC TCGACGAAAT TGTGAGGCTC ACGTCCATCG ACAAGTGGTT TCTGTACAAG 
ATGCGTGACA TTTTAAACAT GGATAAGACA CTGAAAGGGC TTAACAGTGA CTCTGTTACA 
GAAGAAACTC TGAGAAAGGC CAAGGAGATT GGGTTCTCAG ACAAGCAGAT TTCAAAATGT 
TTGGGACTGA CTGAAGCCCA GACAAGAGAG CTGAGGTTAA AGAAAAACAT CCACCCTTGG 
GTTAAACAGA TTGATACATT GGCTGCAGAA TACCCATCAG TAACAAACTA CCTGTATGTT 
ACCTACAACG GCCAGGAGCA CGATATCAAA TTTGATGAAC ATGGAATAAT GGTTCTGGGC 
TGTGGTCCAT ACCACATTGG CAGCAGTGTG GAATTTGATT GGTGTGCTGT CTCCAGTATC 
CGTACACTGC GCCAACTTGG CAAGAAGACA GTGGTGGTGA ATTGTAACCC AGAGACTGTG 
AGCACTGATT TTGATGAGTG TGACAAACTC TACTTTGAAG AGCTGTCTTT GGAGAGAATC 
CTAGACATCT ACCACCAGGA GGCATGTAAT GGCTGCATCA TATCAGTTGG CGGCCAGATT 
CCAAACAACT TGGCGGTCCC GCTGTACAAG AATGGTGTCA AGATCATGGG TACAAGCCCT 
CTGCAGATCG ACAGGGCTGA GGATCGCTCC ATCTTCTCAG CTGTCTTAGA TGAGCTAAAG 
GTGGCCCAGG CTCCATGGAA AGCTGTGAAC ACTTTGAATG AAGCACTGGA ATTTGCAAAC 
TCTGTGGGCT ACCCCTGCTT ACTGAGACCT TCCTATGTTT TGAGTGGGTC TGCCATGAAC 
GTGGTATTCT CTGAGGATGA GATGAAAAGG TTTCTTGAGG AGGCCACTCG AGTCTCTCAG 
GAACACCCGG TGGTGCTGAC CAAGTTTGTG GAGGGGGCCC GGGAAGTAGA GATGGACGCT 
GTTGGCAAAG AAGGACGGGT CATCTCCCAT GCCATCTCTG AACATGTTGA AGATGCAGGT 
GTCCACTCAG GAGATGCCAC CCTGATGCTA CCTACACAGA CCATCAGCCA AGGAGCCATT 
GAAAAGGTGA AGGATGCCAC ACGAAAGATT GCAAAAGCCT TTGCCATCTC TGGGCCATTC 
AATGTCCAGT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGCAA TCTGAGAGCA 
TCAAGATCCT TCCCCTTTGT CTCCAAGACT CTTGGGGTGG ACTTCATTGA CGTGGCTACA 
AAGGTGATGA TCGGGGAGAG CATTGATGAG AAGCGACTTC CCACGCTGGA GCAGCCCATC 
ATTCCCTCTG ACTATGTTGC CATTAAGGCT CCAATGTTTT CCTGGCCCCG ACTGAGAGAT 
GCTGACCCTA TTCTGAGATG TGAGATGGCT TCTACTGGAG AGGTGGCGTG CTTCGGCGAG 
GGCATTCATA CCGCCTTCCT AAAGGCCATG CTGTCTACAG GGTTTAAGAT ACCTCAGAAG 
GGCATTCTGA TTGGAATCCA GCAATCATTC CGTCCAAGAT TCCTTGGTGT TGCTGAGCAG 
TTGCACAATG AAGGTTTCAA GCTTTTTGCC ACAGAAGCTA CATCAGACTG GCTCAATGCC 
AACAATGTCC CTGCCACCCC TGTGGCTTGG CCATCTCAGG AAGGACAGAA TCCCAGCCTC 
TCTTCCATCA GAAAGTTGAT AAGAGATGGA AGCATTGACC TAGTGATTAA CCTCCCCAAT 
AACAACACCA AATTTGTCCA TGATAATTAC GTGATTCGGA GGACAGCTGT GGACAGTGGA 
ATTGCTCTCC TCACCAATTT CCAGGTGACC AAACTTTTTG CTGAAGCTGT GCAGAAATCT 
CGCACTGTGG ACTCCAAGAG TCTGTTCCAC TACAGGCAGT ACAGTGCTGG AAAGGCCGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074278.1
CDS	139..4641
Translation	MTRILTACKVVKTLKSGFGFANVTTKRQWDFSRPGIRLLSVKAKTAHIVLEDGTKMKGYSFGHPSSVAGEVVFNTGLGGYPEALTDPAYKGQILTMANPIIGNGGAPDTTARDELGLNKYMESDGIKVAGLLVLNYSNDYNHWLATKSLGQWLQEEKVPAIYGVDTRMLTKIIRDKGTMLGKIEFEGQSVDFVDPNKQNLIAEVSTKDVKVFGKGNPTKVVAVDCGIKNNVIRLLVKRGAEVHLVPWNHDFTQMEYDGLLIAGGPGNPALAQPLIQNVKKILESDRKEPLFGISTGNIITGLAAGAKSYKMSMANRGQNQPVLNITNRQAFITAQNHGYALDNTLPAGWKPLFVNVNDQTNEGIMHESKPFFAVQFHPEVSPGPTDTEYLFDSFFSLIKKGKGTTITSVLPKPALVASRVEVSKVLILGSGGLSIGQAGEFDYSGSQAVKAMKEENVKTVLMNPNIASVQTNEVGLKQADAVYFLPITPQFVTEVIKAERPDGLILGMGGQTALNCGVELFKRGVLKEYGVKVLGTSVESIMATEDRQLFSDKLNEINEKIAPSFAVESMEDALKAADTIGYPVMIRSAYALGGLGSGICPNKETLIDLGTKAFAMTNQILVERSVTGWKEIEYEVVRDADDNCVTVCNMENVDAMGVHTGDSVVVAPAQTLSNAEFQMLRRTSVNVVRHLGIVGECNIQFALHPTSMEYCIIEVNARLSRSSALASKATGYPLAFIAAKIALGIPLPEIKNVVSGKTSACFEPSLDYMVTKIPRWDLDRFHGTSSRIGSSMKSVGEVMAIGRTFEESFQKALRMCHPSVDGFTPRLPMNKEWPANLDLKKELSEPSSTRIYAIAKALENNMSLDEIVRLTSIDKWFLYKMRDILNMDKTLKGLNSDSVTEETLRKAKEIGFSDKQISKCLGLTEAQTRELRLKKNIHPWVKQIDTLAAEYPSVTNYLYVTYNGQEHDIKFDEHGIMVLGCGPYHIGSSVEFDWCAVSSIRTLRQLGKKTVVVNCNPETVSTDFDECDKLYFEELSLERILDIYHQEACNGCIISVGGQIPNNLAVPLYKNGVKIMGTSPLQIDRAEDRSIFSAVLDELKVAQAPWKAVNTLNEALEFANSVGYPCLLRPSYVLSGSAMNVVFSEDEMKRFLEEATRVSQEHPVVLTKFVEGAREVEMDAVGKEGRVISHAISEHVEDAGVHSGDATLMLPTQTISQGAIEKVKDATRKIAKAFAISGPFNVQFLVKGNDVLVIECNLRASRSFPFVSKTLGVDFIDVATKVMIGESIDEKRLPTLEQPIIPSDYVAIKAPMFSWPRLRDADPILRCEMASTGEVACFGEGIHTAFLKAMLSTGFKIPQKGILIGIQQSFRPRFLGVAEQLHNEGFKLFATEATSDWLNANNVPATPVAWPSQEGQNPSLSSIRKLIRDGSIDLVINLPNNNTKFVHDNYVIRRTAVDSGIALLTNFQVTKLFAEAVQKSRTVDSKSLFHYRQYSAGKAA

Target ORF information:

RefSeq Version	NM_001080809.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus carbamoyl-phosphate synthetase 1 (Cps1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001080809.2

ORF Insert Sequence:

ATGACGAGGA TTTTGACAGC TTGCAAAGTG GTGAAGACCC TGAAGAGTGG CTTTGGTTTT 
GCCAATGTGA CTACGAAGCG ACAGTGGGAC TTCTCCAGAC CTGGCATCAG GCTCCTTTCT 
GTGAAGGCAA AGACAGCACA CATTGTTCTG GAAGATGGAA CTAAGATGAA AGGCTACTCC 
TTTGGCCATC CCTCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCTT AGGAGGGTAC 
CCGGAAGCAC TTACTGATCC CGCCTACAAG GGGCAGATCC TCACCATGGC CAACCCTATC 
ATTGGGAACG GTGGGGCTCC CGACACCACT GCCCGAGACG AACTGGGACT GAATAAGTAC 
ATGGAGTCTG ATGGGATCAA GGTGGCAGGT CTGCTGGTTC TGAATTACAG TAATGACTAT 
AACCACTGGC TGGCTACCAA GAGTCTGGGT CAATGGCTGC AGGAGGAAAA GGTCCCTGCA 
ATTTATGGAG TGGATACAAG AATGCTGACT AAAATAATTC GGGATAAGGG TACCATGCTT 
GGGAAGATTG AATTTGAAGG CCAGTCTGTG GATTTTGTGG ATCCCAATAA GCAGAATTTG 
ATTGCTGAGG TTTCAACCAA GGATGTCAAG GTGTTTGGCA AAGGAAACCC CACGAAAGTG 
GTAGCTGTGG ACTGTGGGAT AAAAAACAAT GTCATCCGCC TGCTAGTTAA GCGAGGAGCT 
GAAGTGCATT TGGTTCCCTG GAATCACGAC TTCACCCAGA TGGAGTATGA TGGACTTTTG 
ATTGCTGGGG GACCTGGGAA TCCAGCTCTT GCACAGCCAC TAATTCAGAA TGTGAAAAAG 
ATTTTGGAGA GTGACCGCAA AGAGCCATTG TTTGGAATCA GTACAGGAAA CATAATAACA 
GGATTGGCTG CTGGTGCCAA ATCCTACAAG ATGTCCATGG CCAACAGAGG ACAGAACCAA 
CCTGTTTTGA ATATCACAAA CAGACAGGCT TTCATAACTG CTCAGAATCA TGGCTATGCT 
CTGGACAACA CACTCCCTGC TGGCTGGAAA CCACTGTTTG TGAATGTCAA TGATCAAACA 
AACGAGGGGA TTATGCATGA GAGCAAACCT TTCTTCGCTG TGCAGTTCCA CCCAGAGGTC 
AGCCCGGGGC CAACAGACAC TGAGTACCTA TTTGATTCCT TCTTCTCCCT GATAAAGAAG 
GGCAAAGGCA CCACCATTAC CTCAGTTCTG CCCAAGCCAG CGTTGGTGGC ATCTCGTGTC 
GAGGTTTCCA AGGTCCTCAT CCTGGGATCA GGAGGTCTGT CCATTGGTCA GGCAGGTGAA 
TTTGATTACT CGGGATCTCA AGCTGTGAAA GCCATGAAGG AAGAAAATGT CAAAACAGTC 
CTGATGAACC CGAACATCGC ATCCGTGCAG ACCAACGAGG TGGGATTGAA GCAGGCAGAT 
GCAGTCTACT TTCTCCCTAT TACCCCCCAG TTTGTCACAG AAGTTATCAA GGCTGAACGG 
CCTGATGGGC TGATTCTGGG CATGGGTGGC CAGACAGCTC TGAACTGTGG AGTGGAACTA 
TTCAAGAGGG GTGTGCTCAA GGAATATGGG GTGAAGGTCT TGGGCACATC AGTTGAGTCC 
ATTATGGCCA CAGAAGACAG GCAGCTGTTC TCAGACAAGC TGAATGAGAT CAACGAGAAG 
ATCGCACCCA GTTTTGCAGT GGAATCAATG GAGGATGCCT TGAAGGCAGC AGACACCATT 
GGCTACCCTG TGATGATTCG GTCTGCATAT GCTCTGGGTG GCTTAGGCTC TGGCATCTGT 
CCCAATAAGG AGACCTTAAT AGACCTTGGC ACAAAAGCAT TTGCTATGAC CAACCAGATC 
CTGGTGGAGC GGTCAGTGAC AGGTTGGAAA GAAATAGAAT ATGAAGTTGT CCGAGATGCA 
GATGATAACT GTGTCACAGT CTGTAACATG GAAAATGTTG ACGCCATGGG TGTTCACACA 
GGTGACTCGG TTGTCGTGGC CCCAGCCCAG ACACTCTCCA ATGCAGAGTT CCAGATGTTG 
AGACGCACTT CAGTCAATGT TGTTCGTCAC TTGGGCATTG TGGGCGAATG CAACATTCAG 
TTTGCTCTTC ACCCCACTTC CATGGAATAC TGTATCATTG AAGTGAATGC CAGGCTGTCC 
CGGAGTTCTG CCCTGGCCTC CAAGGCCACT GGCTACCCAC TGGCATTCAT TGCAGCAAAG 
ATTGCTCTAG GAATCCCACT TCCAGAAATC AAGAATGTTG TATCTGGGAA GACCTCAGCC 
TGCTTCGAAC CTAGCCTGGA TTACATGGTG ACCAAGATTC CTCGCTGGGA TCTTGACCGT 
TTCCATGGAA CATCCAGCCG AATTGGTAGC TCAATGAAAA GTGTGGGCGA GGTCATGGCC 
ATTGGTCGCA CCTTTGAGGA AAGTTTCCAG AAGGCTCTGA GGATGTGCCA TCCATCTGTG 
GATGGGTTCA CTCCCCGGCT CCCAATGAAT AAGGAATGGC CAGCAAACCT GGATCTGAAG 
AAAGAGCTGT CTGAGCCCTC CAGCACCCGC ATCTACGCCA TTGCTAAGGC CTTGGAGAAC 
AACATGTCCC TCGACGAAAT TGTGAGGCTC ACGTCCATCG ACAAGTGGTT TCTGTACAAG 
ATGCGTGACA TTTTAAACAT GGATAAGACA CTGAAAGGGC TTAACAGTGA CTCTGTTACA 
GAAGAAACTC TGAGAAAGGC CAAGGAGATT GGGTTCTCAG ACAAGCAGAT TTCAAAATGT 
TTGGGACTGA CTGAAGCCCA GACAAGAGAG CTGAGGTTAA AGAAAAACAT CCACCCTTGG 
GTTAAACAGA TTGATACATT GGCTGCAGAA TACCCATCAG TAACAAACTA CCTGTATGTT 
ACCTACAACG GCCAGGAGCA CGATATCAAA TTTGATGAAC ATGGAATAAT GGTTCTGGGC 
TGTGGTCCAT ACCACATTGG CAGCAGTGTG GAATTTGATT GGTGTGCTGT CTCCAGTATC 
CGTACACTGC GCCAACTTGG CAAGAAGACA GTGGTGGTGA ATTGTAACCC AGAGACTGTG 
AGCACTGATT TTGATGAGTG TGACAAACTC TACTTTGAAG AGCTGTCTTT GGAGAGAATC 
CTAGACATCT ACCACCAGGA GGCATGTAAT GGCTGCATCA TATCAGTTGG CGGCCAGATT 
CCAAACAACT TGGCGGTCCC GCTGTACAAG AATGGTGTCA AGATCATGGG TACAAGCCCT 
CTGCAGATCG ACAGGGCTGA GGATCGCTCC ATCTTCTCAG CTGTCTTAGA TGAGCTAAAG 
GTGGCCCAGG CTCCATGGAA AGCTGTGAAC ACTTTGAATG AAGCACTGGA ATTTGCAAAC 
TCTGTGGGCT ACCCCTGCTT ACTGAGACCT TCCTATGTTT TGAGTGGGTC TGCCATGAAC 
GTGGTATTCT CTGAGGATGA GATGAAAAGG TTTCTTGAGG AGGCCACTCG AGTCTCTCAG 
GAACACCCGG TGGTGCTGAC CAAGTTTGTG GAGGGGGCCC GGGAAGTAGA GATGGACGCT 
GTTGGCAAAG AAGGACGGGT CATCTCCCAT GCCATCTCTG AACATGTTGA AGATGCAGGT 
GTCCACTCAG GAGATGCCAC CCTGATGCTA CCTACACAGA CCATCAGCCA AGGAGCCATT 
GAAAAGGTGA AGGATGCCAC ACGAAAGATT GCAAAAGCCT TTGCCATCTC TGGGCCATTC 
AATGTCCAGT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGCAA TCTGAGAGCA 
TCAAGATCCT TCCCCTTTGT CTCCAAGACT CTTGGGGTGG ACTTCATTGA CGTGGCTACA 
AAGGTGATGA TCGGGGAGAG CATTGATGAG AAGCGACTTC CCACGCTGGA GCAGCCCATC 
ATTCCCTCTG ACTATGTTGC CATTAAGGCT CCAATGTTTT CCTGGCCCCG ACTGAGAGAT 
GCTGACCCTA TTCTGAGATG TGAGATGGCT TCTACTGGAG AGGTGGCGTG CTTCGGCGAG 
GGCATTCATA CCGCCTTCCT AAAGGCCATG CTGTCTACAG GGTTTAAGAT ACCTCAGAAG 
GGCATTCTGA TTGGAATCCA GCAATCATTC CGTCCAAGAT TCCTTGGTGT TGCTGAGCAG 
TTGCACAATG AAGGTTTCAA GCTTTTTGCC ACAGAAGCTA CATCAGACTG GCTCAATGCC 
AACAATGTCC CTGCCACCCC TGTGGCTTGG CCATCTCAGG AAGGACAGAA TCCCAGCCTC 
TCTTCCATCA GAAAGTTGAT AAGAGATGGA AGCATTGACC TAGTGATTAA CCTCCCCAAT 
AACAACACCA AATTTGTCCA TGATAATTAC GTGATTCGGA GGACAGCTGT GGACAGTGGA 
ATTGCTCTCC TCACCAATTT CCAGGTGACC AAACTTTTTG CTGAAGCTGT GCAGAAATCT 
CGCACTGTGG ACTCCAAGAG TCTGTTCCAC TACAGGCAGT ACAGTGCTGG AAAGGCCGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cps1 ( XM_011238500.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011236802.1 Mus musculus carbamoyl-phosphate synthetase 1 (Cps1), transcript variant X1, mRNA.

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CloneID	OMu16468
Clone ID Related Accession (Same CDS sequence)	XM_011238500.1 , NM_001080809.2
Accession Version	XM_011238500.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4503bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	carbamoyl-phosphate synthase [ammonia], mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGAGGA TTTTGACAGC TTGCAAAGTG GTGAAGACCC TGAAGAGTGG CTTTGGTTTT 
GCCAATGTGA CTACGAAGCG ACAGTGGGAC TTCTCCAGAC CTGGCATCAG GCTCCTTTCT 
GTGAAGGCAA AGACAGCACA CATTGTTCTG GAAGATGGAA CTAAGATGAA AGGCTACTCC 
TTTGGCCATC CCTCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCTT AGGAGGGTAC 
CCGGAAGCAC TTACTGATCC CGCCTACAAG GGGCAGATCC TCACCATGGC CAACCCTATC 
ATTGGGAACG GTGGGGCTCC CGACACCACT GCCCGAGACG AACTGGGACT GAATAAGTAC 
ATGGAGTCTG ATGGGATCAA GGTGGCAGGT CTGCTGGTTC TGAATTACAG TAATGACTAT 
AACCACTGGC TGGCTACCAA GAGTCTGGGT CAATGGCTGC AGGAGGAAAA GGTCCCTGCA 
ATTTATGGAG TGGATACAAG AATGCTGACT AAAATAATTC GGGATAAGGG TACCATGCTT 
GGGAAGATTG AATTTGAAGG CCAGTCTGTG GATTTTGTGG ATCCCAATAA GCAGAATTTG 
ATTGCTGAGG TTTCAACCAA GGATGTCAAG GTGTTTGGCA AAGGAAACCC CACGAAAGTG 
GTAGCTGTGG ACTGTGGGAT AAAAAACAAT GTCATCCGCC TGCTAGTTAA GCGAGGAGCT 
GAAGTGCATT TGGTTCCCTG GAATCACGAC TTCACCCAGA TGGAGTATGA TGGACTTTTG 
ATTGCTGGGG GACCTGGGAA TCCAGCTCTT GCACAGCCAC TAATTCAGAA TGTGAAAAAG 
ATTTTGGAGA GTGACCGCAA AGAGCCATTG TTTGGAATCA GTACAGGAAA CATAATAACA 
GGATTGGCTG CTGGTGCCAA ATCCTACAAG ATGTCCATGG CCAACAGAGG ACAGAACCAA 
CCTGTTTTGA ATATCACAAA CAGACAGGCT TTCATAACTG CTCAGAATCA TGGCTATGCT 
CTGGACAACA CACTCCCTGC TGGCTGGAAA CCACTGTTTG TGAATGTCAA TGATCAAACA 
AACGAGGGGA TTATGCATGA GAGCAAACCT TTCTTCGCTG TGCAGTTCCA CCCAGAGGTC 
AGCCCGGGGC CAACAGACAC TGAGTACCTA TTTGATTCCT TCTTCTCCCT GATAAAGAAG 
GGCAAAGGCA CCACCATTAC CTCAGTTCTG CCCAAGCCAG CGTTGGTGGC ATCTCGTGTC 
GAGGTTTCCA AGGTCCTCAT CCTGGGATCA GGAGGTCTGT CCATTGGTCA GGCAGGTGAA 
TTTGATTACT CGGGATCTCA AGCTGTGAAA GCCATGAAGG AAGAAAATGT CAAAACAGTC 
CTGATGAACC CGAACATCGC ATCCGTGCAG ACCAACGAGG TGGGATTGAA GCAGGCAGAT 
GCAGTCTACT TTCTCCCTAT TACCCCCCAG TTTGTCACAG AAGTTATCAA GGCTGAACGG 
CCTGATGGGC TGATTCTGGG CATGGGTGGC CAGACAGCTC TGAACTGTGG AGTGGAACTA 
TTCAAGAGGG GTGTGCTCAA GGAATATGGG GTGAAGGTCT TGGGCACATC AGTTGAGTCC 
ATTATGGCCA CAGAAGACAG GCAGCTGTTC TCAGACAAGC TGAATGAGAT CAACGAGAAG 
ATCGCACCCA GTTTTGCAGT GGAATCAATG GAGGATGCCT TGAAGGCAGC AGACACCATT 
GGCTACCCTG TGATGATTCG GTCTGCATAT GCTCTGGGTG GCTTAGGCTC TGGCATCTGT 
CCCAATAAGG AGACCTTAAT AGACCTTGGC ACAAAAGCAT TTGCTATGAC CAACCAGATC 
CTGGTGGAGC GGTCAGTGAC AGGTTGGAAA GAAATAGAAT ATGAAGTTGT CCGAGATGCA 
GATGATAACT GTGTCACAGT CTGTAACATG GAAAATGTTG ACGCCATGGG TGTTCACACA 
GGTGACTCGG TTGTCGTGGC CCCAGCCCAG ACACTCTCCA ATGCAGAGTT CCAGATGTTG 
AGACGCACTT CAGTCAATGT TGTTCGTCAC TTGGGCATTG TGGGCGAATG CAACATTCAG 
TTTGCTCTTC ACCCCACTTC CATGGAATAC TGTATCATTG AAGTGAATGC CAGGCTGTCC 
CGGAGTTCTG CCCTGGCCTC CAAGGCCACT GGCTACCCAC TGGCATTCAT TGCAGCAAAG 
ATTGCTCTAG GAATCCCACT TCCAGAAATC AAGAATGTTG TATCTGGGAA GACCTCAGCC 
TGCTTCGAAC CTAGCCTGGA TTACATGGTG ACCAAGATTC CTCGCTGGGA TCTTGACCGT 
TTCCATGGAA CATCCAGCCG AATTGGTAGC TCAATGAAAA GTGTGGGCGA GGTCATGGCC 
ATTGGTCGCA CCTTTGAGGA AAGTTTCCAG AAGGCTCTGA GGATGTGCCA TCCATCTGTG 
GATGGGTTCA CTCCCCGGCT CCCAATGAAT AAGGAATGGC CAGCAAACCT GGATCTGAAG 
AAAGAGCTGT CTGAGCCCTC CAGCACCCGC ATCTACGCCA TTGCTAAGGC CTTGGAGAAC 
AACATGTCCC TCGACGAAAT TGTGAGGCTC ACGTCCATCG ACAAGTGGTT TCTGTACAAG 
ATGCGTGACA TTTTAAACAT GGATAAGACA CTGAAAGGGC TTAACAGTGA CTCTGTTACA 
GAAGAAACTC TGAGAAAGGC CAAGGAGATT GGGTTCTCAG ACAAGCAGAT TTCAAAATGT 
TTGGGACTGA CTGAAGCCCA GACAAGAGAG CTGAGGTTAA AGAAAAACAT CCACCCTTGG 
GTTAAACAGA TTGATACATT GGCTGCAGAA TACCCATCAG TAACAAACTA CCTGTATGTT 
ACCTACAACG GCCAGGAGCA CGATATCAAA TTTGATGAAC ATGGAATAAT GGTTCTGGGC 
TGTGGTCCAT ACCACATTGG CAGCAGTGTG GAATTTGATT GGTGTGCTGT CTCCAGTATC 
CGTACACTGC GCCAACTTGG CAAGAAGACA GTGGTGGTGA ATTGTAACCC AGAGACTGTG 
AGCACTGATT TTGATGAGTG TGACAAACTC TACTTTGAAG AGCTGTCTTT GGAGAGAATC 
CTAGACATCT ACCACCAGGA GGCATGTAAT GGCTGCATCA TATCAGTTGG CGGCCAGATT 
CCAAACAACT TGGCGGTCCC GCTGTACAAG AATGGTGTCA AGATCATGGG TACAAGCCCT 
CTGCAGATCG ACAGGGCTGA GGATCGCTCC ATCTTCTCAG CTGTCTTAGA TGAGCTAAAG 
GTGGCCCAGG CTCCATGGAA AGCTGTGAAC ACTTTGAATG AAGCACTGGA ATTTGCAAAC 
TCTGTGGGCT ACCCCTGCTT ACTGAGACCT TCCTATGTTT TGAGTGGGTC TGCCATGAAC 
GTGGTATTCT CTGAGGATGA GATGAAAAGG TTTCTTGAGG AGGCCACTCG AGTCTCTCAG 
GAACACCCGG TGGTGCTGAC CAAGTTTGTG GAGGGGGCCC GGGAAGTAGA GATGGACGCT 
GTTGGCAAAG AAGGACGGGT CATCTCCCAT GCCATCTCTG AACATGTTGA AGATGCAGGT 
GTCCACTCAG GAGATGCCAC CCTGATGCTA CCTACACAGA CCATCAGCCA AGGAGCCATT 
GAAAAGGTGA AGGATGCCAC ACGAAAGATT GCAAAAGCCT TTGCCATCTC TGGGCCATTC 
AATGTCCAGT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGCAA TCTGAGAGCA 
TCAAGATCCT TCCCCTTTGT CTCCAAGACT CTTGGGGTGG ACTTCATTGA CGTGGCTACA 
AAGGTGATGA TCGGGGAGAG CATTGATGAG AAGCGACTTC CCACGCTGGA GCAGCCCATC 
ATTCCCTCTG ACTATGTTGC CATTAAGGCT CCAATGTTTT CCTGGCCCCG ACTGAGAGAT 
GCTGACCCTA TTCTGAGATG TGAGATGGCT TCTACTGGAG AGGTGGCGTG CTTCGGCGAG 
GGCATTCATA CCGCCTTCCT AAAGGCCATG CTGTCTACAG GGTTTAAGAT ACCTCAGAAG 
GGCATTCTGA TTGGAATCCA GCAATCATTC CGTCCAAGAT TCCTTGGTGT TGCTGAGCAG 
TTGCACAATG AAGGTTTCAA GCTTTTTGCC ACAGAAGCTA CATCAGACTG GCTCAATGCC 
AACAATGTCC CTGCCACCCC TGTGGCTTGG CCATCTCAGG AAGGACAGAA TCCCAGCCTC 
TCTTCCATCA GAAAGTTGAT AAGAGATGGA AGCATTGACC TAGTGATTAA CCTCCCCAAT 
AACAACACCA AATTTGTCCA TGATAATTAC GTGATTCGGA GGACAGCTGT GGACAGTGGA 
ATTGCTCTCC TCACCAATTT CCAGGTGACC AAACTTTTTG CTGAAGCTGT GCAGAAATCT 
CGCACTGTGG ACTCCAAGAG TCTGTTCCAC TACAGGCAGT ACAGTGCTGG AAAGGCCGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011236802.1
CDS	36..4538
Translation	MTRILTACKVVKTLKSGFGFANVTTKRQWDFSRPGIRLLSVKAKTAHIVLEDGTKMKGYSFGHPSSVAGEVVFNTGLGGYPEALTDPAYKGQILTMANPIIGNGGAPDTTARDELGLNKYMESDGIKVAGLLVLNYSNDYNHWLATKSLGQWLQEEKVPAIYGVDTRMLTKIIRDKGTMLGKIEFEGQSVDFVDPNKQNLIAEVSTKDVKVFGKGNPTKVVAVDCGIKNNVIRLLVKRGAEVHLVPWNHDFTQMEYDGLLIAGGPGNPALAQPLIQNVKKILESDRKEPLFGISTGNIITGLAAGAKSYKMSMANRGQNQPVLNITNRQAFITAQNHGYALDNTLPAGWKPLFVNVNDQTNEGIMHESKPFFAVQFHPEVSPGPTDTEYLFDSFFSLIKKGKGTTITSVLPKPALVASRVEVSKVLILGSGGLSIGQAGEFDYSGSQAVKAMKEENVKTVLMNPNIASVQTNEVGLKQADAVYFLPITPQFVTEVIKAERPDGLILGMGGQTALNCGVELFKRGVLKEYGVKVLGTSVESIMATEDRQLFSDKLNEINEKIAPSFAVESMEDALKAADTIGYPVMIRSAYALGGLGSGICPNKETLIDLGTKAFAMTNQILVERSVTGWKEIEYEVVRDADDNCVTVCNMENVDAMGVHTGDSVVVAPAQTLSNAEFQMLRRTSVNVVRHLGIVGECNIQFALHPTSMEYCIIEVNARLSRSSALASKATGYPLAFIAAKIALGIPLPEIKNVVSGKTSACFEPSLDYMVTKIPRWDLDRFHGTSSRIGSSMKSVGEVMAIGRTFEESFQKALRMCHPSVDGFTPRLPMNKEWPANLDLKKELSEPSSTRIYAIAKALENNMSLDEIVRLTSIDKWFLYKMRDILNMDKTLKGLNSDSVTEETLRKAKEIGFSDKQISKCLGLTEAQTRELRLKKNIHPWVKQIDTLAAEYPSVTNYLYVTYNGQEHDIKFDEHGIMVLGCGPYHIGSSVEFDWCAVSSIRTLRQLGKKTVVVNCNPETVSTDFDECDKLYFEELSLERILDIYHQEACNGCIISVGGQIPNNLAVPLYKNGVKIMGTSPLQIDRAEDRSIFSAVLDELKVAQAPWKAVNTLNEALEFANSVGYPCLLRPSYVLSGSAMNVVFSEDEMKRFLEEATRVSQEHPVVLTKFVEGAREVEMDAVGKEGRVISHAISEHVEDAGVHSGDATLMLPTQTISQGAIEKVKDATRKIAKAFAISGPFNVQFLVKGNDVLVIECNLRASRSFPFVSKTLGVDFIDVATKVMIGESIDEKRLPTLEQPIIPSDYVAIKAPMFSWPRLRDADPILRCEMASTGEVACFGEGIHTAFLKAMLSTGFKIPQKGILIGIQQSFRPRFLGVAEQLHNEGFKLFATEATSDWLNANNVPATPVAWPSQEGQNPSLSSIRKLIRDGSIDLVINLPNNNTKFVHDNYVIRRTAVDSGIALLTNFQVTKLFAEAVQKSRTVDSKSLFHYRQYSAGKAA

Target ORF information:

RefSeq Version	XM_011238500.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus carbamoyl-phosphate synthetase 1 (Cps1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011238500.1

ORF Insert Sequence:

ATGACGAGGA TTTTGACAGC TTGCAAAGTG GTGAAGACCC TGAAGAGTGG CTTTGGTTTT 
GCCAATGTGA CTACGAAGCG ACAGTGGGAC TTCTCCAGAC CTGGCATCAG GCTCCTTTCT 
GTGAAGGCAA AGACAGCACA CATTGTTCTG GAAGATGGAA CTAAGATGAA AGGCTACTCC 
TTTGGCCATC CCTCCTCTGT TGCTGGTGAA GTGGTTTTTA ATACTGGCTT AGGAGGGTAC 
CCGGAAGCAC TTACTGATCC CGCCTACAAG GGGCAGATCC TCACCATGGC CAACCCTATC 
ATTGGGAACG GTGGGGCTCC CGACACCACT GCCCGAGACG AACTGGGACT GAATAAGTAC 
ATGGAGTCTG ATGGGATCAA GGTGGCAGGT CTGCTGGTTC TGAATTACAG TAATGACTAT 
AACCACTGGC TGGCTACCAA GAGTCTGGGT CAATGGCTGC AGGAGGAAAA GGTCCCTGCA 
ATTTATGGAG TGGATACAAG AATGCTGACT AAAATAATTC GGGATAAGGG TACCATGCTT 
GGGAAGATTG AATTTGAAGG CCAGTCTGTG GATTTTGTGG ATCCCAATAA GCAGAATTTG 
ATTGCTGAGG TTTCAACCAA GGATGTCAAG GTGTTTGGCA AAGGAAACCC CACGAAAGTG 
GTAGCTGTGG ACTGTGGGAT AAAAAACAAT GTCATCCGCC TGCTAGTTAA GCGAGGAGCT 
GAAGTGCATT TGGTTCCCTG GAATCACGAC TTCACCCAGA TGGAGTATGA TGGACTTTTG 
ATTGCTGGGG GACCTGGGAA TCCAGCTCTT GCACAGCCAC TAATTCAGAA TGTGAAAAAG 
ATTTTGGAGA GTGACCGCAA AGAGCCATTG TTTGGAATCA GTACAGGAAA CATAATAACA 
GGATTGGCTG CTGGTGCCAA ATCCTACAAG ATGTCCATGG CCAACAGAGG ACAGAACCAA 
CCTGTTTTGA ATATCACAAA CAGACAGGCT TTCATAACTG CTCAGAATCA TGGCTATGCT 
CTGGACAACA CACTCCCTGC TGGCTGGAAA CCACTGTTTG TGAATGTCAA TGATCAAACA 
AACGAGGGGA TTATGCATGA GAGCAAACCT TTCTTCGCTG TGCAGTTCCA CCCAGAGGTC 
AGCCCGGGGC CAACAGACAC TGAGTACCTA TTTGATTCCT TCTTCTCCCT GATAAAGAAG 
GGCAAAGGCA CCACCATTAC CTCAGTTCTG CCCAAGCCAG CGTTGGTGGC ATCTCGTGTC 
GAGGTTTCCA AGGTCCTCAT CCTGGGATCA GGAGGTCTGT CCATTGGTCA GGCAGGTGAA 
TTTGATTACT CGGGATCTCA AGCTGTGAAA GCCATGAAGG AAGAAAATGT CAAAACAGTC 
CTGATGAACC CGAACATCGC ATCCGTGCAG ACCAACGAGG TGGGATTGAA GCAGGCAGAT 
GCAGTCTACT TTCTCCCTAT TACCCCCCAG TTTGTCACAG AAGTTATCAA GGCTGAACGG 
CCTGATGGGC TGATTCTGGG CATGGGTGGC CAGACAGCTC TGAACTGTGG AGTGGAACTA 
TTCAAGAGGG GTGTGCTCAA GGAATATGGG GTGAAGGTCT TGGGCACATC AGTTGAGTCC 
ATTATGGCCA CAGAAGACAG GCAGCTGTTC TCAGACAAGC TGAATGAGAT CAACGAGAAG 
ATCGCACCCA GTTTTGCAGT GGAATCAATG GAGGATGCCT TGAAGGCAGC AGACACCATT 
GGCTACCCTG TGATGATTCG GTCTGCATAT GCTCTGGGTG GCTTAGGCTC TGGCATCTGT 
CCCAATAAGG AGACCTTAAT AGACCTTGGC ACAAAAGCAT TTGCTATGAC CAACCAGATC 
CTGGTGGAGC GGTCAGTGAC AGGTTGGAAA GAAATAGAAT ATGAAGTTGT CCGAGATGCA 
GATGATAACT GTGTCACAGT CTGTAACATG GAAAATGTTG ACGCCATGGG TGTTCACACA 
GGTGACTCGG TTGTCGTGGC CCCAGCCCAG ACACTCTCCA ATGCAGAGTT CCAGATGTTG 
AGACGCACTT CAGTCAATGT TGTTCGTCAC TTGGGCATTG TGGGCGAATG CAACATTCAG 
TTTGCTCTTC ACCCCACTTC CATGGAATAC TGTATCATTG AAGTGAATGC CAGGCTGTCC 
CGGAGTTCTG CCCTGGCCTC CAAGGCCACT GGCTACCCAC TGGCATTCAT TGCAGCAAAG 
ATTGCTCTAG GAATCCCACT TCCAGAAATC AAGAATGTTG TATCTGGGAA GACCTCAGCC 
TGCTTCGAAC CTAGCCTGGA TTACATGGTG ACCAAGATTC CTCGCTGGGA TCTTGACCGT 
TTCCATGGAA CATCCAGCCG AATTGGTAGC TCAATGAAAA GTGTGGGCGA GGTCATGGCC 
ATTGGTCGCA CCTTTGAGGA AAGTTTCCAG AAGGCTCTGA GGATGTGCCA TCCATCTGTG 
GATGGGTTCA CTCCCCGGCT CCCAATGAAT AAGGAATGGC CAGCAAACCT GGATCTGAAG 
AAAGAGCTGT CTGAGCCCTC CAGCACCCGC ATCTACGCCA TTGCTAAGGC CTTGGAGAAC 
AACATGTCCC TCGACGAAAT TGTGAGGCTC ACGTCCATCG ACAAGTGGTT TCTGTACAAG 
ATGCGTGACA TTTTAAACAT GGATAAGACA CTGAAAGGGC TTAACAGTGA CTCTGTTACA 
GAAGAAACTC TGAGAAAGGC CAAGGAGATT GGGTTCTCAG ACAAGCAGAT TTCAAAATGT 
TTGGGACTGA CTGAAGCCCA GACAAGAGAG CTGAGGTTAA AGAAAAACAT CCACCCTTGG 
GTTAAACAGA TTGATACATT GGCTGCAGAA TACCCATCAG TAACAAACTA CCTGTATGTT 
ACCTACAACG GCCAGGAGCA CGATATCAAA TTTGATGAAC ATGGAATAAT GGTTCTGGGC 
TGTGGTCCAT ACCACATTGG CAGCAGTGTG GAATTTGATT GGTGTGCTGT CTCCAGTATC 
CGTACACTGC GCCAACTTGG CAAGAAGACA GTGGTGGTGA ATTGTAACCC AGAGACTGTG 
AGCACTGATT TTGATGAGTG TGACAAACTC TACTTTGAAG AGCTGTCTTT GGAGAGAATC 
CTAGACATCT ACCACCAGGA GGCATGTAAT GGCTGCATCA TATCAGTTGG CGGCCAGATT 
CCAAACAACT TGGCGGTCCC GCTGTACAAG AATGGTGTCA AGATCATGGG TACAAGCCCT 
CTGCAGATCG ACAGGGCTGA GGATCGCTCC ATCTTCTCAG CTGTCTTAGA TGAGCTAAAG 
GTGGCCCAGG CTCCATGGAA AGCTGTGAAC ACTTTGAATG AAGCACTGGA ATTTGCAAAC 
TCTGTGGGCT ACCCCTGCTT ACTGAGACCT TCCTATGTTT TGAGTGGGTC TGCCATGAAC 
GTGGTATTCT CTGAGGATGA GATGAAAAGG TTTCTTGAGG AGGCCACTCG AGTCTCTCAG 
GAACACCCGG TGGTGCTGAC CAAGTTTGTG GAGGGGGCCC GGGAAGTAGA GATGGACGCT 
GTTGGCAAAG AAGGACGGGT CATCTCCCAT GCCATCTCTG AACATGTTGA AGATGCAGGT 
GTCCACTCAG GAGATGCCAC CCTGATGCTA CCTACACAGA CCATCAGCCA AGGAGCCATT 
GAAAAGGTGA AGGATGCCAC ACGAAAGATT GCAAAAGCCT TTGCCATCTC TGGGCCATTC 
AATGTCCAGT TTCTTGTCAA AGGAAATGAT GTCTTGGTGA TTGAGTGCAA TCTGAGAGCA 
TCAAGATCCT TCCCCTTTGT CTCCAAGACT CTTGGGGTGG ACTTCATTGA CGTGGCTACA 
AAGGTGATGA TCGGGGAGAG CATTGATGAG AAGCGACTTC CCACGCTGGA GCAGCCCATC 
ATTCCCTCTG ACTATGTTGC CATTAAGGCT CCAATGTTTT CCTGGCCCCG ACTGAGAGAT 
GCTGACCCTA TTCTGAGATG TGAGATGGCT TCTACTGGAG AGGTGGCGTG CTTCGGCGAG 
GGCATTCATA CCGCCTTCCT AAAGGCCATG CTGTCTACAG GGTTTAAGAT ACCTCAGAAG 
GGCATTCTGA TTGGAATCCA GCAATCATTC CGTCCAAGAT TCCTTGGTGT TGCTGAGCAG 
TTGCACAATG AAGGTTTCAA GCTTTTTGCC ACAGAAGCTA CATCAGACTG GCTCAATGCC 
AACAATGTCC CTGCCACCCC TGTGGCTTGG CCATCTCAGG AAGGACAGAA TCCCAGCCTC 
TCTTCCATCA GAAAGTTGAT AAGAGATGGA AGCATTGACC TAGTGATTAA CCTCCCCAAT 
AACAACACCA AATTTGTCCA TGATAATTAC GTGATTCGGA GGACAGCTGT GGACAGTGGA 
ATTGCTCTCC TCACCAATTT CCAGGTGACC AAACTTTTTG CTGAAGCTGT GCAGAAATCT 
CGCACTGTGG ACTCCAAGAG TCTGTTCCAC TACAGGCAGT ACAGTGCTGG AAAGGCCGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The RSPO-LGR4/5-ZNRF3/RNF43 module controls liver zonation and size.
Nature cell biology18(5)467-79(2016 05)
Planas-Paz L,Orsini V,Boulter L,Calabrese D,Pikiolek M,Nigsch F,Xie Y,Roma G,Donovan A,Marti P,Beckmann N,Dill MT,Carbone W,Bergling S,Isken A,Mueller M,Kinzel B,Yang Y,Mao X,Nicholson TB,Zamponi R,Capodieci P,Valdez R,Rivera D,Loew A,Ukomadu C,Terracciano LM,Bouwmeester T,Cong F,Heim MH,Forbes SJ,Ruffner H,Tchorz JS

Overexpression of SIRT5 confirms its involvement in deacetylation and activation of carbamoyl phosphate synthetase 1.
Biochemical and biophysical research communications393(1)73-8(2010 Feb)
Ogura M,Nakamura Y,Tanaka D,Zhuang X,Fujita Y,Obara A,Hamasaki A,Hosokawa M,Inagaki N

SIRT5 Deacetylates carbamoyl phosphate synthetase 1 and regulates the urea cycle.
Cell137(3)560-70(2009 May)
Nakagawa T,Lomb DJ,Haigis MC,Guarente L

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Cps1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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