Zfp60 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Zfp60
Entrez Gene ID	22718
Full Name	zinc finger protein 60
Synonyms	6330516O17Rik,AI426106,AI449354,Mfg-3,Mfg3
General protein information	Preferred Names zinc finger protein 60 Names zinc finger protein 60 zfp-60 zinc finger protein Mfg-3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7 A3\|7 15.97 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_009560.2	NP_033586.2	zinc finger protein 60
NM_029531.2	NP_083807.2	zinc finger protein 60

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Zfp60	NP_033586.2
Rattus norvegicus (Norway rat)	LOC102554136	XP_006228667.1
Rattus norvegicus (Norway rat)	LOC100910046	XP_006228664.1

Gene Ontology
Bibliography

Related articles in PubMed

Resolution of cell fate decisions revealed by single-cell gene expression analysis from zygote to blastocyst.
Guo G, Huss M, Tong GQ, Wang C, Li Sun L, Clarke ND, Robson P
Developmental cell18(4)675-85(2010 Apr)

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Yokoyama S, Ito Y, Ueno-Kudoh H, Shimizu H, Uchibe K, Albini S, Mitsuoka K, Miyaki S, Kiso M, Nagai A, Hikata T, Osada T, Fukuda N, Yamashita S, Harada D, Mezzano V, Kasai M, Puri PL, Hayashizaki Y, Okado H, Hashimoto M, Asahara H
Developmental cell17(6)836-48(2009 Dec)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

The zinc finger transcription factor Zfp60 is a negative regulator of cartilage differentiation.
Ganss B, Kobayashi H
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research17(12)2151-60(2002 Dec)

Zfp60, a mouse zinc finger gene expressed transiently during in vitro muscle differentiation.
Perez M, Rompato G, Corbi N, De Gregorio L, Dragani TA, Passananti C
FEBS letters387(2-3)117-21(1996 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

role as negative regulator of cartilage differentiation
Title: The zinc finger transcription factor Zfp60 is a negative regulator of cartilage differentiation.

The following Zfp60 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Zfp60 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu23453	NM_009560.2 Latest version!	Mus musculus zinc finger protein 60 (Zfp60), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OMu23453	NM_029531.2 Latest version!	Mus musculus zinc finger protein 60 (Zfp60), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Zfp60 ( NM_009560.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033586.2 Mus musculus zinc finger protein 60 (Zfp60), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu23453
Clone ID Related Accession (Same CDS sequence)	NM_009560.2 , NM_029531.2
Accession Version	NM_009560.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Mus musculus(house mouse)
Product	zinc finger protein 60
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AA718695.1, CB526626.1, AC074312.64 and CAAA01066021.1. On Feb 4, 2006 this sequence version replaced NM_009560.1. Transcript Variant: This variant (1) represents the longer transcript. ##Evidence-Data-START## Transcript exon combination :: SRR1660815.41789.1, AK028717.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCAACT CTAGTTCTCA ACACATGGTC TGTGGGTCAG TGACATTTAG GGATGTGGCT 
GTTGACTTCT CTCAGGAGGA GTGGGCCTGC CTGGATGCTA CTCAGAAGGT CTTATACAGG 
AACATAATGT TGGAGACCTA CAGCAACCTG GTCGCAGTGG TGGGACGTTG CATTCCCAAG 
CCAGATCTGA TCGTCTTACT AGAGCAGGAG AAAGAGCCCT GGATGGCTGT GAAGAAAGAA 
ACAGGCAGGC CGAGCCAAGG TTTGGAGACA GGTTTTGAAG CTGAAAATAG ATCTCCAAAG 
AATCATGTTT ATAATAAAAA GTTACCCAAA CAAACCATAC AGCAATTAAG TAAAACTTCG 
GACGTCCAGG GCGTCAGTGT TAGTAATGGC CCTGGCTATA GTGTAATCAA GGAGCCACAG 
AATTATCAAG AAGGAGATGC TAATCGAAAT ATTACTAACA AGAAAGAAAT GTCTACTTAC 
ACTAGCAAGA CTCTTGCTCA CAATAAAGAA AAACCGTATA AATGTAAAGA CTGTGGGAAG 
TGCTTTGGTT GTAAGTCAAA CCTTCATCAG CATGAGAGTA TTCACACTGG AGAGAAGCCC 
TATGAATGTA AGGACTGTGG GAAGACCTTC AGACTCCCTC AGATGCTTTC AAGACATCAG 
AAGTCTCACA GTGATGAGAG ACCTTTTGAG TGTAATATAT GTGGAAAGTC TTTTCATCTT 
CCTACCCTGC TTCAGTACCA TAAAAATATT CATACAGGTT TAAAACCATT TGAATGTGAG 
GAGTGTGGGA AGTCCTTCAA CCGCATCTCT ACCCTTTTTC AGCATAGGAC TATTCATGCT 
GGTATGAAAC CGTATAAATG TAATGTGTGT GGGAAAGCCT TTAATCGTCG CTCAAACCTC 
CTGCAACATC AGAAAATTCA TTCTGAGGAC AGACCTTTTC ATTGTAAGGT GTGTGGAAAG 
GCCTTCACTG TGCTGGCTCA GCTTACTCGG CACGAGAACA TTCACACTGA AGACAAATCG 
TTTGAATGTA AGCAGTGTGG TAAGATATTT AGTAATGGCT CTTACCTCTT ACGACACTAC 
GATACTCACA CCAATGAGAA ACCCTTTGAG TGTAATATAT GTGGCAAGGC TTTTAGGCTT 
CATCTATACC TTTCTGAGCA TCAGAAAACT CACACCGATG AGAAGCCTTT CAAGTGTAAG 
CTGTGTGAGT CAGCCTTCAG ACGTAAGTAC CAGCTCAGTG AACATCAGAG AATTCACACT 
GATGTGAAAC CCTATCAGTG CAAGGATTGT GGGAAATTCT TTCGTCGACG ATCAAATTTT 
ACTGAACATC AGAGTATTCA CACTGGAAAG AAACCCTTTG AGTGTAAGGA CTGTGGGAAG 
GTCTTTAGAC TAAATATACA TCTCATTCGA CATCAGAGAT TTCATAGTGA TGAGAAGCCC 
TTTGAATGTA AAGAATGTGG AAAGGCTTTT CATTTTTCCA GCCAGCTTAA TAACCATAAA 
ACCAGTCATA CAGGTCAGAC ACCTTTTGAA TGTAAAGAAT GTGGGAAGTC GTTTAAGCGT 
GTCTCCAGCC TTGTGGAACA TAGGATTATT CACAGTGGTG TGAAACCATA TAAATGTAAT 
GCGTGTGGAA GAGCCTTCAA TCGTCGCTCA AACCTCATGC AACATGAGAA AATTCATTCT 
GATGAGAGAC CCTTTGAGTG TAAGGACTGT GGAAAGGCCT TCACTGTTCT GGCTCAGCTC 
ACACGGCACC AGACGATTCA TAATGGAAAG AAATCATATG AATGTGAGCA GTGTGGGTCA 
GCCTTCAGAC TCCCGTACCA ACTCACTCAA CATCAGAGAA TTCATTATGA TGTGAAGCCA 
TTTCAGTGCA AGGAATGTGG AAAGGGCTTT GTTCGTAGTA CAGGCCTTAG AATTCATGAA 
AGGATCCACA CTGGTGAGAA GCCCTTTCAG TGTAAGGAGT GTGGAGAAGC CTTTCAGTAT 
CATTACCAAT TTCTTGGACA CTTTAGAATT CATACTGGCA AGAACCCTTA TGAATGTAGT 
GAATGTGGGA AGTACTTTAC TTATGGTCGA GACCTTAAAG TACATCAGAG TATTCACAAT 
CTTGAGAAAC CGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033586.2
CDS	277..2391
Translation	MANSSSQHMVCGSVTFRDVAVDFSQEEWACLDATQKVLYRNIMLETYSNLVAVVGRCIPKPDLIVLLEQEKEPWMAVKKETGRPSQGLETGFEAENRSPKNHVYNKKLPKQTIQQLSKTSDVQGVSVSNGPGYSVIKEPQNYQEGDANRNITNKKEMSTYTSKTLAHNKEKPYKCKDCGKCFGCKSNLHQHESIHTGEKPYECKDCGKTFRLPQMLSRHQKSHSDERPFECNICGKSFHLPTLLQYHKNIHTGLKPFECEECGKSFNRISTLFQHRTIHAGMKPYKCNVCGKAFNRRSNLLQHQKIHSEDRPFHCKVCGKAFTVLAQLTRHENIHTEDKSFECKQCGKIFSNGSYLLRHYDTHTNEKPFECNICGKAFRLHLYLSEHQKTHTDEKPFKCKLCESAFRRKYQLSEHQRIHTDVKPYQCKDCGKFFRRRSNFTEHQSIHTGKKPFECKDCGKVFRLNIHLIRHQRFHSDEKPFECKECGKAFHFSSQLNNHKTSHTGQTPFECKECGKSFKRVSSLVEHRIIHSGVKPYKCNACGRAFNRRSNLMQHEKIHSDERPFECKDCGKAFTVLAQLTRHQTIHNGKKSYECEQCGSAFRLPYQLTQHQRIHYDVKPFQCKECGKGFVRSTGLRIHERIHTGEKPFQCKECGEAFQYHYQFLGHFRIHTGKNPYECSECGKYFTYGRDLKVHQSIHNLEKP

Target ORF information:

RefSeq Version	NM_009560.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus zinc finger protein 60 (Zfp60), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009560.2

ORF Insert Sequence:

ATGGCCAACT CTAGTTCTCA ACACATGGTC TGTGGGTCAG TGACATTTAG GGATGTGGCT 
GTTGACTTCT CTCAGGAGGA GTGGGCCTGC CTGGATGCTA CTCAGAAGGT CTTATACAGG 
AACATAATGT TGGAGACCTA CAGCAACCTG GTCGCAGTGG TGGGACGTTG CATTCCCAAG 
CCAGATCTGA TCGTCTTACT AGAGCAGGAG AAAGAGCCCT GGATGGCTGT GAAGAAAGAA 
ACAGGCAGGC CGAGCCAAGG TTTGGAGACA GGTTTTGAAG CTGAAAATAG ATCTCCAAAG 
AATCATGTTT ATAATAAAAA GTTACCCAAA CAAACCATAC AGCAATTAAG TAAAACTTCG 
GACGTCCAGG GCGTCAGTGT TAGTAATGGC CCTGGCTATA GTGTAATCAA GGAGCCACAG 
AATTATCAAG AAGGAGATGC TAATCGAAAT ATTACTAACA AGAAAGAAAT GTCTACTTAC 
ACTAGCAAGA CTCTTGCTCA CAATAAAGAA AAACCGTATA AATGTAAAGA CTGTGGGAAG 
TGCTTTGGTT GTAAGTCAAA CCTTCATCAG CATGAGAGTA TTCACACTGG AGAGAAGCCC 
TATGAATGTA AGGACTGTGG GAAGACCTTC AGACTCCCTC AGATGCTTTC AAGACATCAG 
AAGTCTCACA GTGATGAGAG ACCTTTTGAG TGTAATATAT GTGGAAAGTC TTTTCATCTT 
CCTACCCTGC TTCAGTACCA TAAAAATATT CATACAGGTT TAAAACCATT TGAATGTGAG 
GAGTGTGGGA AGTCCTTCAA CCGCATCTCT ACCCTTTTTC AGCATAGGAC TATTCATGCT 
GGTATGAAAC CGTATAAATG TAATGTGTGT GGGAAAGCCT TTAATCGTCG CTCAAACCTC 
CTGCAACATC AGAAAATTCA TTCTGAGGAC AGACCTTTTC ATTGTAAGGT GTGTGGAAAG 
GCCTTCACTG TGCTGGCTCA GCTTACTCGG CACGAGAACA TTCACACTGA AGACAAATCG 
TTTGAATGTA AGCAGTGTGG TAAGATATTT AGTAATGGCT CTTACCTCTT ACGACACTAC 
GATACTCACA CCAATGAGAA ACCCTTTGAG TGTAATATAT GTGGCAAGGC TTTTAGGCTT 
CATCTATACC TTTCTGAGCA TCAGAAAACT CACACCGATG AGAAGCCTTT CAAGTGTAAG 
CTGTGTGAGT CAGCCTTCAG ACGTAAGTAC CAGCTCAGTG AACATCAGAG AATTCACACT 
GATGTGAAAC CCTATCAGTG CAAGGATTGT GGGAAATTCT TTCGTCGACG ATCAAATTTT 
ACTGAACATC AGAGTATTCA CACTGGAAAG AAACCCTTTG AGTGTAAGGA CTGTGGGAAG 
GTCTTTAGAC TAAATATACA TCTCATTCGA CATCAGAGAT TTCATAGTGA TGAGAAGCCC 
TTTGAATGTA AAGAATGTGG AAAGGCTTTT CATTTTTCCA GCCAGCTTAA TAACCATAAA 
ACCAGTCATA CAGGTCAGAC ACCTTTTGAA TGTAAAGAAT GTGGGAAGTC GTTTAAGCGT 
GTCTCCAGCC TTGTGGAACA TAGGATTATT CACAGTGGTG TGAAACCATA TAAATGTAAT 
GCGTGTGGAA GAGCCTTCAA TCGTCGCTCA AACCTCATGC AACATGAGAA AATTCATTCT 
GATGAGAGAC CCTTTGAGTG TAAGGACTGT GGAAAGGCCT TCACTGTTCT GGCTCAGCTC 
ACACGGCACC AGACGATTCA TAATGGAAAG AAATCATATG AATGTGAGCA GTGTGGGTCA 
GCCTTCAGAC TCCCGTACCA ACTCACTCAA CATCAGAGAA TTCATTATGA TGTGAAGCCA 
TTTCAGTGCA AGGAATGTGG AAAGGGCTTT GTTCGTAGTA CAGGCCTTAG AATTCATGAA 
AGGATCCACA CTGGTGAGAA GCCCTTTCAG TGTAAGGAGT GTGGAGAAGC CTTTCAGTAT 
CATTACCAAT TTCTTGGACA CTTTAGAATT CATACTGGCA AGAACCCTTA TGAATGTAGT 
GAATGTGGGA AGTACTTTAC TTATGGTCGA GACCTTAAAG TACATCAGAG TATTCACAAT 
CTTGAGAAAC CGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Zfp60 ( NM_029531.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_083807.2 Mus musculus zinc finger protein 60 (Zfp60), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu23453
Clone ID Related Accession (Same CDS sequence)	NM_009560.2 , NM_029531.2
Accession Version	NM_029531.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Mus musculus(house mouse)
Product	zinc finger protein 60
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK149305.1 and CAAA01066021.1. On or before May 4, 2006 this sequence version replaced XM_979639.1, NM_029531.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. ##Evidence-Data-START## Transcript exon combination :: AK149305.1, SRR1660819.78980.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCAACT CTAGTTCTCA ACACATGGTC TGTGGGTCAG TGACATTTAG GGATGTGGCT 
GTTGACTTCT CTCAGGAGGA GTGGGCCTGC CTGGATGCTA CTCAGAAGGT CTTATACAGG 
AACATAATGT TGGAGACCTA CAGCAACCTG GTCGCAGTGG TGGGACGTTG CATTCCCAAG 
CCAGATCTGA TCGTCTTACT AGAGCAGGAG AAAGAGCCCT GGATGGCTGT GAAGAAAGAA 
ACAGGCAGGC CGAGCCAAGG TTTGGAGACA GGTTTTGAAG CTGAAAATAG ATCTCCAAAG 
AATCATGTTT ATAATAAAAA GTTACCCAAA CAAACCATAC AGCAATTAAG TAAAACTTCG 
GACGTCCAGG GCGTCAGTGT TAGTAATGGC CCTGGCTATA GTGTAATCAA GGAGCCACAG 
AATTATCAAG AAGGAGATGC TAATCGAAAT ATTACTAACA AGAAAGAAAT GTCTACTTAC 
ACTAGCAAGA CTCTTGCTCA CAATAAAGAA AAACCGTATA AATGTAAAGA CTGTGGGAAG 
TGCTTTGGTT GTAAGTCAAA CCTTCATCAG CATGAGAGTA TTCACACTGG AGAGAAGCCC 
TATGAATGTA AGGACTGTGG GAAGACCTTC AGACTCCCTC AGATGCTTTC AAGACATCAG 
AAGTCTCACA GTGATGAGAG ACCTTTTGAG TGTAATATAT GTGGAAAGTC TTTTCATCTT 
CCTACCCTGC TTCAGTACCA TAAAAATATT CATACAGGTT TAAAACCATT TGAATGTGAG 
GAGTGTGGGA AGTCCTTCAA CCGCATCTCT ACCCTTTTTC AGCATAGGAC TATTCATGCT 
GGTATGAAAC CGTATAAATG TAATGTGTGT GGGAAAGCCT TTAATCGTCG CTCAAACCTC 
CTGCAACATC AGAAAATTCA TTCTGAGGAC AGACCTTTTC ATTGTAAGGT GTGTGGAAAG 
GCCTTCACTG TGCTGGCTCA GCTTACTCGG CACGAGAACA TTCACACTGA AGACAAATCG 
TTTGAATGTA AGCAGTGTGG TAAGATATTT AGTAATGGCT CTTACCTCTT ACGACACTAC 
GATACTCACA CCAATGAGAA ACCCTTTGAG TGTAATATAT GTGGCAAGGC TTTTAGGCTT 
CATCTATACC TTTCTGAGCA TCAGAAAACT CACACCGATG AGAAGCCTTT CAAGTGTAAG 
CTGTGTGAGT CAGCCTTCAG ACGTAAGTAC CAGCTCAGTG AACATCAGAG AATTCACACT 
GATGTGAAAC CCTATCAGTG CAAGGATTGT GGGAAATTCT TTCGTCGACG ATCAAATTTT 
ACTGAACATC AGAGTATTCA CACTGGAAAG AAACCCTTTG AGTGTAAGGA CTGTGGGAAG 
GTCTTTAGAC TAAATATACA TCTCATTCGA CATCAGAGAT TTCATAGTGA TGAGAAGCCC 
TTTGAATGTA AAGAATGTGG AAAGGCTTTT CATTTTTCCA GCCAGCTTAA TAACCATAAA 
ACCAGTCATA CAGGTCAGAC ACCTTTTGAA TGTAAAGAAT GTGGGAAGTC GTTTAAGCGT 
GTCTCCAGCC TTGTGGAACA TAGGATTATT CACAGTGGTG TGAAACCATA TAAATGTAAT 
GCGTGTGGAA GAGCCTTCAA TCGTCGCTCA AACCTCATGC AACATGAGAA AATTCATTCT 
GATGAGAGAC CCTTTGAGTG TAAGGACTGT GGAAAGGCCT TCACTGTTCT GGCTCAGCTC 
ACACGGCACC AGACGATTCA TAATGGAAAG AAATCATATG AATGTGAGCA GTGTGGGTCA 
GCCTTCAGAC TCCCGTACCA ACTCACTCAA CATCAGAGAA TTCATTATGA TGTGAAGCCA 
TTTCAGTGCA AGGAATGTGG AAAGGGCTTT GTTCGTAGTA CAGGCCTTAG AATTCATGAA 
AGGATCCACA CTGGTGAGAA GCCCTTTCAG TGTAAGGAGT GTGGAGAAGC CTTTCAGTAT 
CATTACCAAT TTCTTGGACA CTTTAGAATT CATACTGGCA AGAACCCTTA TGAATGTAGT 
GAATGTGGGA AGTACTTTAC TTATGGTCGA GACCTTAAAG TACATCAGAG TATTCACAAT 
CTTGAGAAAC CGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_083807.2
CDS	83..2197
Translation	MANSSSQHMVCGSVTFRDVAVDFSQEEWACLDATQKVLYRNIMLETYSNLVAVVGRCIPKPDLIVLLEQEKEPWMAVKKETGRPSQGLETGFEAENRSPKNHVYNKKLPKQTIQQLSKTSDVQGVSVSNGPGYSVIKEPQNYQEGDANRNITNKKEMSTYTSKTLAHNKEKPYKCKDCGKCFGCKSNLHQHESIHTGEKPYECKDCGKTFRLPQMLSRHQKSHSDERPFECNICGKSFHLPTLLQYHKNIHTGLKPFECEECGKSFNRISTLFQHRTIHAGMKPYKCNVCGKAFNRRSNLLQHQKIHSEDRPFHCKVCGKAFTVLAQLTRHENIHTEDKSFECKQCGKIFSNGSYLLRHYDTHTNEKPFECNICGKAFRLHLYLSEHQKTHTDEKPFKCKLCESAFRRKYQLSEHQRIHTDVKPYQCKDCGKFFRRRSNFTEHQSIHTGKKPFECKDCGKVFRLNIHLIRHQRFHSDEKPFECKECGKAFHFSSQLNNHKTSHTGQTPFECKECGKSFKRVSSLVEHRIIHSGVKPYKCNACGRAFNRRSNLMQHEKIHSDERPFECKDCGKAFTVLAQLTRHQTIHNGKKSYECEQCGSAFRLPYQLTQHQRIHYDVKPFQCKECGKGFVRSTGLRIHERIHTGEKPFQCKECGEAFQYHYQFLGHFRIHTGKNPYECSECGKYFTYGRDLKVHQSIHNLEKP

Target ORF information:

RefSeq Version	NM_029531.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus zinc finger protein 60 (Zfp60), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_029531.2

ORF Insert Sequence:

ATGGCCAACT CTAGTTCTCA ACACATGGTC TGTGGGTCAG TGACATTTAG GGATGTGGCT 
GTTGACTTCT CTCAGGAGGA GTGGGCCTGC CTGGATGCTA CTCAGAAGGT CTTATACAGG 
AACATAATGT TGGAGACCTA CAGCAACCTG GTCGCAGTGG TGGGACGTTG CATTCCCAAG 
CCAGATCTGA TCGTCTTACT AGAGCAGGAG AAAGAGCCCT GGATGGCTGT GAAGAAAGAA 
ACAGGCAGGC CGAGCCAAGG TTTGGAGACA GGTTTTGAAG CTGAAAATAG ATCTCCAAAG 
AATCATGTTT ATAATAAAAA GTTACCCAAA CAAACCATAC AGCAATTAAG TAAAACTTCG 
GACGTCCAGG GCGTCAGTGT TAGTAATGGC CCTGGCTATA GTGTAATCAA GGAGCCACAG 
AATTATCAAG AAGGAGATGC TAATCGAAAT ATTACTAACA AGAAAGAAAT GTCTACTTAC 
ACTAGCAAGA CTCTTGCTCA CAATAAAGAA AAACCGTATA AATGTAAAGA CTGTGGGAAG 
TGCTTTGGTT GTAAGTCAAA CCTTCATCAG CATGAGAGTA TTCACACTGG AGAGAAGCCC 
TATGAATGTA AGGACTGTGG GAAGACCTTC AGACTCCCTC AGATGCTTTC AAGACATCAG 
AAGTCTCACA GTGATGAGAG ACCTTTTGAG TGTAATATAT GTGGAAAGTC TTTTCATCTT 
CCTACCCTGC TTCAGTACCA TAAAAATATT CATACAGGTT TAAAACCATT TGAATGTGAG 
GAGTGTGGGA AGTCCTTCAA CCGCATCTCT ACCCTTTTTC AGCATAGGAC TATTCATGCT 
GGTATGAAAC CGTATAAATG TAATGTGTGT GGGAAAGCCT TTAATCGTCG CTCAAACCTC 
CTGCAACATC AGAAAATTCA TTCTGAGGAC AGACCTTTTC ATTGTAAGGT GTGTGGAAAG 
GCCTTCACTG TGCTGGCTCA GCTTACTCGG CACGAGAACA TTCACACTGA AGACAAATCG 
TTTGAATGTA AGCAGTGTGG TAAGATATTT AGTAATGGCT CTTACCTCTT ACGACACTAC 
GATACTCACA CCAATGAGAA ACCCTTTGAG TGTAATATAT GTGGCAAGGC TTTTAGGCTT 
CATCTATACC TTTCTGAGCA TCAGAAAACT CACACCGATG AGAAGCCTTT CAAGTGTAAG 
CTGTGTGAGT CAGCCTTCAG ACGTAAGTAC CAGCTCAGTG AACATCAGAG AATTCACACT 
GATGTGAAAC CCTATCAGTG CAAGGATTGT GGGAAATTCT TTCGTCGACG ATCAAATTTT 
ACTGAACATC AGAGTATTCA CACTGGAAAG AAACCCTTTG AGTGTAAGGA CTGTGGGAAG 
GTCTTTAGAC TAAATATACA TCTCATTCGA CATCAGAGAT TTCATAGTGA TGAGAAGCCC 
TTTGAATGTA AAGAATGTGG AAAGGCTTTT CATTTTTCCA GCCAGCTTAA TAACCATAAA 
ACCAGTCATA CAGGTCAGAC ACCTTTTGAA TGTAAAGAAT GTGGGAAGTC GTTTAAGCGT 
GTCTCCAGCC TTGTGGAACA TAGGATTATT CACAGTGGTG TGAAACCATA TAAATGTAAT 
GCGTGTGGAA GAGCCTTCAA TCGTCGCTCA AACCTCATGC AACATGAGAA AATTCATTCT 
GATGAGAGAC CCTTTGAGTG TAAGGACTGT GGAAAGGCCT TCACTGTTCT GGCTCAGCTC 
ACACGGCACC AGACGATTCA TAATGGAAAG AAATCATATG AATGTGAGCA GTGTGGGTCA 
GCCTTCAGAC TCCCGTACCA ACTCACTCAA CATCAGAGAA TTCATTATGA TGTGAAGCCA 
TTTCAGTGCA AGGAATGTGG AAAGGGCTTT GTTCGTAGTA CAGGCCTTAG AATTCATGAA 
AGGATCCACA CTGGTGAGAA GCCCTTTCAG TGTAAGGAGT GTGGAGAAGC CTTTCAGTAT 
CATTACCAAT TTCTTGGACA CTTTAGAATT CATACTGGCA AGAACCCTTA TGAATGTAGT 
GAATGTGGGA AGTACTTTAC TTATGGTCGA GACCTTAAAG TACATCAGAG TATTCACAAT 
CTTGAGAAAC CGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Resolution of cell fate decisions revealed by single-cell gene expression analysis from zygote to blastocyst.
Developmental cell18(4)675-85(2010 Apr)
Guo G,Huss M,Tong GQ,Wang C,Li Sun L,Clarke ND,Robson P

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Developmental cell17(6)836-48(2009 Dec)
Yokoyama S,Ito Y,Ueno-Kudoh H,Shimizu H,Uchibe K,Albini S,Mitsuoka K,Miyaki S,Kiso M,Nagai A,Hikata T,Osada T,Fukuda N,Yamashita S,Harada D,Mezzano V,Kasai M,Puri PL,Hayashizaki Y,Okado H,Hashimoto M,Asahara H

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

The zinc finger transcription factor Zfp60 is a negative regulator of cartilage differentiation.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research17(12)2151-60(2002 Dec)
Ganss B,Kobayashi H

Zfp60, a mouse zinc finger gene expressed transiently during in vitro muscle differentiation.
FEBS letters387(2-3)117-21(1996 Jun)
Perez M,Rompato G,Corbi N,De Gregorio L,Dragani TA,Passananti C

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Zfp60 cDNA ORF clone, Mus musculus(house mouse)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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