Mpp4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mpp4
Entrez Gene ID	227157
Full Name	membrane protein, palmitoylated 4 (MAGUK p55 subfamily member 4)
Synonyms	Dlg6
General protein information	Preferred Names membrane protein, palmitoylated 4 (MAGUK p55 subfamily member 4) Names MAGUK p55 subfamily member 4 discs large homolog 6 mDLG6 membrane palmitoylated protein 4
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1\|1 C1.3

mRNA and Protein(s)

mRNA	Protein	Name
NM_145143.3	NP_660125.2	MAGUK p55 subfamily member 4 isoform 2
NM_001164682.1	NP_001158154.1	MAGUK p55 subfamily member 4 isoform 1

Pathways from BioSystems

Homologies

	MPP4	XP_001100100.1
Canis lupus familiaris (dog)	MPP4	XP_005640792.1
Homo sapiens (human)	MPP4	NP_149055.1
Bos taurus (cattle)	MPP4	XP_002685536.1
Danio rerio (zebrafish)	LOC100331226	XP_002662343.2
Mus musculus (house mouse)	Mpp4	NP_001158154.1
Gallus gallus (chicken)	MPP4	XP_001233047.2
Xenopus tropicalis (tropical clawed frog)	mpp4	XP_002936584.2
Danio rerio (zebrafish)	LOC556594	XP_684526.5
Pan troglodytes (chimpanzee)	MPP4	XP_001172377.3
Rattus norvegicus (Norway rat)	Mpp4	NP_067088.2

Gene Ontology
Bibliography

Related articles in PubMed

Motor phenotypes and molecular networks associated with germline deficiency of Ciz1.
Xiao J, Vemula SR, Xue Y, Khan MM, Kuruvilla KP, Marquez-Lona EM, Cobb MR, LeDoux MS
Experimental neurology283(Pt A)110-20(2016 Sep)

The first murine zygotic transcription is promiscuous and uncoupled from splicing and 3' processing.
Abe K, Yamamoto R, Franke V, Cao M, Suzuki Y, Suzuki MG, Vlahovicek K, Svoboda P, Schultz RM, Aoki F
The EMBO journal34(11)1523-37(2015 Jun)

PSD95beta regulates plasma membrane Ca2+ pump localization at the photoreceptor synapse.
Aartsen WM, Arsanto JP, Chauvin JP, Vos RM, Versteeg I, Cardozo BN, Bivic AL, Wijnholds J
Molecular and cellular neurosciences41(2)156-65(2009 Jun)

Mpp4 is required for proper localization of plasma membrane calcium ATPases and maintenance of calcium homeostasis at the rod photoreceptor synaptic terminals.
Yang J, Pawlyk B, Wen XH, Adamian M, Soloviev M, Michaud N, Zhao Y, Sandberg MA, Makino CL, Li T
Human molecular genetics16(9)1017-29(2007 May)

Cracking the egg: molecular dynamics and evolutionary aspects of the transition from the fully grown oocyte to embryo.
Evsikov AV, Graber JH, Brockman JM, Hampl A, Holbrook AE, Singh P, Eppig JJ, Solter D, Knowles BB
Genes & development20(19)2713-27(2006 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that both scaffold proteins PSD95beta and MPP4 are essential for the modulation of PMCA levels at the presynaptic plasma membrane and thereby influence the photoreceptor synaptic calcium handling.
Title: PSD95beta regulates plasma membrane Ca2+ pump localization at the photoreceptor synapse.

Results show that Mpp4 organizes a presynaptic protein complex and has a role in modulating Ca(2+) homeostasis and synaptic transmission in rod photoreceptors.
Title: Mpp4 is required for proper localization of plasma membrane calcium ATPases and maintenance of calcium homeostasis at the rod photoreceptor synaptic terminals.

These findings suggest that Mpp4 coordinates Psd95/Veli3 assembly and maintenance at synaptic membranes. Mpp4 is a critical recruitment factor to organize scaffolds at the photoreceptor synapse.
Title: Mpp4 recruits Psd95 and Veli3 towards the photoreceptor synapse.

Association of Veli3 with MPP4 suggests that the MAGUKs recruit Veli3 and its binding partners to different cellular regions of the retina where they may participate in the organization of specialized intercellular junctions.
Title: Membrane-associated guanylate kinase proteins MPP4 and MPP5 associate with Veli3 at distinct intercellular junctions of the neurosensory retina.

Results describe the temporal and spatial expression patterns of mouse membrane palmitoylated protein 4 (Mpp4) gene.
Title: Developmental and tissue expression patterns of mouse Mpp4 gene.

The following Mpp4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mpp4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu10022	NM_001164682.1 Latest version!	Mus musculus membrane protein, palmitoylated 4 (MAGUK p55 subfamily member 4) (Mpp4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$384.30 $549.00
OMu11346	NM_145143.3 Latest version!	Mus musculus membrane protein, palmitoylated 4 (MAGUK p55 subfamily member 4) (Mpp4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mpp4 ( NM_001164682.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001158154.1 Mus musculus membrane protein, palmitoylated 4 (MAGUK p55 subfamily member 4) (Mpp4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu10022
Clone ID Related Accession (Same CDS sequence)	NM_001164682.1
Accession Version	NM_001164682.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	MAGUK p55 subfamily member 4 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DV648961.1, BM945398.1, AK044682.1 and BC061694.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK044682.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164136, SAMN01164141 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGCATT TCCAGAAGGC ACCAGCAGCA GCTACAGATA ATCCTCCAGC AGGGAGGATG 
AGACAGTCTG ACAGAGGAGC AGAGCTGACC AATGAGGACA GAGCTCTTCC CACGCCACCT 
GATCCTGAGA ATGGTCTCTC TGGGATCCTG AGGCTCCTAC TGCAGGAACT GAGCCTTTTC 
TACAGCCGAG ATGTGAATGG TCTGTGTCTC TTATATGACC TCCTCCATTC ACCATGGCTG 
CAGGCTCTGC TCAAGGTCTA CGACTGCCTC CAGAGGTTTA AAGAGAAGAA GCTAGTTCCT 
GATACAACTC ATGCACAGAT CTTAGCCTGT GAGGTCTTGG AGCTGTTACC CAAAGCTTCC 
GGTTCCCCCG AGATCCAAGA GCTAAGACAA GTCCTTCAGG CTCCTCACTG CAAGGCCTTG 
CTGAGTGCCC ATGACACAGT GGCTCAGAAA GACTTTGAAC CCCTTCTCCC CCCACTGCCA 
GACAATATCC CCGACAGTGA AGAAGCAATG AGGATTGTTT GTCTGGTGAA AAACCAACAG 
CCCCTGGGAG CAACCATCAA GCGTCATGAG ATAACAGGGG ATATCCTGGT GGCCAGGGTC 
ATCCATGGCG GGCTGGTGGA GAGGAGCGGG TTGCTGTATG CTGGAGACAA ACTGGTGGAA 
GTGAATGGAG TTTCAGTAGA GGGCCTGGAC CCTGAGCAAG TGATCCATAT TTTGGCTATG 
TCTTGTGGTA CGATCATGTT CAAGGTGATT CCGGTCTCTG CCCCGCCTGT GAGCAGCCAG 
AAGATGGTTT ATGTTCGCGC CATGATTGAT TACTGGCCCC AAGAGGATCC CGACATCCCC 
TGCATGGATG CTGGGCTGCC TTTCCTGAAA GGGGACATTC TACAGATTGT GGACCAGAAT 
GATGCCCTCT GGTGGCAGGC CCGGAAAATC TCAGACCTCA CCATCTGTGC TGGGCTAATC 
CCTTCTAACC ACCTTCTGAA GAGGAAGCAG CGGGAGTTCT GGTGGTCTCA GCCGTACCAA 
CCTCACACCT GCCTCAAATC CACCCGAGCA CTGTCTATGG AGGAAGAAGA CAGCATGAAG 
ATTGATGAGA AGTGTGTGGA AGCAGATGAA GAAACTTTTG AATCTGAGGA ACTCGCAGAA 
GCTAAGGACG AATTTGTCGG CGATGGGCAG AAGTTCTTTA TCGCTGGGTT CCGCCGCAGC 
ATGCGCCTCT GTCGCAGGAA GTCTCACTTC AGCCAGCTGC ATGCCAGCCT GTGCTGCAGC 
TGCAGCTGCT ACAGTGCCGT GGGCGCTCCT TACGAGGAGG TGGTGAGGTA CCAGCGGCAG 
CCGGCGGACA AACACCGCCT CATAGTGCTT GTGGGACCTT CTGGCGTTGG AGTCAATGAA 
CTGAGAAGAC AGCTCATTGG ATGTAACCCC AGCTGTTTTC AAAGTGCTGT GCCACATACC 
ACCCGTTTCC CAAAGAGTTA TGAAATGGAT GGACGTGAGT ATCACTACGT GTCAAGGGAA 
ACATTTGAAA GCCTCATGTA CGGACACAAG ATGCTGGAGT ATGGTGAGTA CAAGGGCCAC 
CTGTACGGAA CCAGTGTGAA CGCTGTACAC GCCGTCCTTG ATGAAGGCAA GATCTGCATC 
ATGGATCTAG AGCCTCAGGA TATTCAGTCG GCTCGAACCC GTGACTTGAA GCCCTACGTT 
ATATTTATTA AGCCACCTAA CACGAGCTCC ATGAGACACT CTCGGAAAAA CGCTAAGATC 
ACCACTGACT ACTATGTGGA CATGAAGTTC AAGGATGAAG ACCTACAAGA GATGGAGGAG 
TTAGCCCAGA AAATGGAGAG TCAGTTTGGC CAGTTTTTTG ATCACGTGAT TGTGAATGAC 
AACTTGCAAG ATGCATGTGG CCAGCTGTTG TCCGCCATAC AGAAGGCTCA GGAAGAACTG 
CAGTGGGTGC CAGAGGCATG GGTTTCCCCT GACACTGAAT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001158154.1
CDS	700..2664
Translation	MPHFQKAPAAATDNPPAGRMRQSDRGAELTNEDRALPTPPDPENGLSGILRLLLQELSLFYSRDVNGLCLLYDLLHSPWLQALLKVYDCLQRFKEKKLVPDTTHAQILACEVLELLPKASGSPEIQELRQVLQAPHCKALLSAHDTVAQKDFEPLLPPLPDNIPDSEEAMRIVCLVKNQQPLGATIKRHEITGDILVARVIHGGLVERSGLLYAGDKLVEVNGVSVEGLDPEQVIHILAMSCGTIMFKVIPVSAPPVSSQKMVYVRAMIDYWPQEDPDIPCMDAGLPFLKGDILQIVDQNDALWWQARKISDLTICAGLIPSNHLLKRKQREFWWSQPYQPHTCLKSTRALSMEEEDSMKIDEKCVEADEETFESEELAEAKDEFVGDGQKFFIAGFRRSMRLCRRKSHFSQLHASLCCSCSCYSAVGAPYEEVVRYQRQPADKHRLIVLVGPSGVGVNELRRQLIGCNPSCFQSAVPHTTRFPKSYEMDGREYHYVSRETFESLMYGHKMLEYGEYKGHLYGTSVNAVHAVLDEGKICIMDLEPQDIQSARTRDLKPYVIFIKPPNTSSMRHSRKNAKITTDYYVDMKFKDEDLQEMEELAQKMESQFGQFFDHVIVNDNLQDACGQLLSAIQKAQEELQWVPEAWVSPDTES

Target ORF information:

RefSeq Version	NM_001164682.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus membrane protein, palmitoylated 4 (MAGUK p55 subfamily member 4) (Mpp4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001164682.1

ORF Insert Sequence:

ATGCCGCATT TCCAGAAGGC ACCAGCAGCA GCTACAGATA ATCCTCCAGC AGGGAGGATG 
AGACAGTCTG ACAGAGGAGC AGAGCTGACC AATGAGGACA GAGCTCTTCC CACGCCACCT 
GATCCTGAGA ATGGTCTCTC TGGGATCCTG AGGCTCCTAC TGCAGGAACT GAGCCTTTTC 
TACAGCCGAG ATGTGAATGG TCTGTGTCTC TTATATGACC TCCTCCATTC ACCATGGCTG 
CAGGCTCTGC TCAAGGTCTA CGACTGCCTC CAGAGGTTTA AAGAGAAGAA GCTAGTTCCT 
GATACAACTC ATGCACAGAT CTTAGCCTGT GAGGTCTTGG AGCTGTTACC CAAAGCTTCC 
GGTTCCCCCG AGATCCAAGA GCTAAGACAA GTCCTTCAGG CTCCTCACTG CAAGGCCTTG 
CTGAGTGCCC ATGACACAGT GGCTCAGAAA GACTTTGAAC CCCTTCTCCC CCCACTGCCA 
GACAATATCC CCGACAGTGA AGAAGCAATG AGGATTGTTT GTCTGGTGAA AAACCAACAG 
CCCCTGGGAG CAACCATCAA GCGTCATGAG ATAACAGGGG ATATCCTGGT GGCCAGGGTC 
ATCCATGGCG GGCTGGTGGA GAGGAGCGGG TTGCTGTATG CTGGAGACAA ACTGGTGGAA 
GTGAATGGAG TTTCAGTAGA GGGCCTGGAC CCTGAGCAAG TGATCCATAT TTTGGCTATG 
TCTTGTGGTA CGATCATGTT CAAGGTGATT CCGGTCTCTG CCCCGCCTGT GAGCAGCCAG 
AAGATGGTTT ATGTTCGCGC CATGATTGAT TACTGGCCCC AAGAGGATCC CGACATCCCC 
TGCATGGATG CTGGGCTGCC TTTCCTGAAA GGGGACATTC TACAGATTGT GGACCAGAAT 
GATGCCCTCT GGTGGCAGGC CCGGAAAATC TCAGACCTCA CCATCTGTGC TGGGCTAATC 
CCTTCTAACC ACCTTCTGAA GAGGAAGCAG CGGGAGTTCT GGTGGTCTCA GCCGTACCAA 
CCTCACACCT GCCTCAAATC CACCCGAGCA CTGTCTATGG AGGAAGAAGA CAGCATGAAG 
ATTGATGAGA AGTGTGTGGA AGCAGATGAA GAAACTTTTG AATCTGAGGA ACTCGCAGAA 
GCTAAGGACG AATTTGTCGG CGATGGGCAG AAGTTCTTTA TCGCTGGGTT CCGCCGCAGC 
ATGCGCCTCT GTCGCAGGAA GTCTCACTTC AGCCAGCTGC ATGCCAGCCT GTGCTGCAGC 
TGCAGCTGCT ACAGTGCCGT GGGCGCTCCT TACGAGGAGG TGGTGAGGTA CCAGCGGCAG 
CCGGCGGACA AACACCGCCT CATAGTGCTT GTGGGACCTT CTGGCGTTGG AGTCAATGAA 
CTGAGAAGAC AGCTCATTGG ATGTAACCCC AGCTGTTTTC AAAGTGCTGT GCCACATACC 
ACCCGTTTCC CAAAGAGTTA TGAAATGGAT GGACGTGAGT ATCACTACGT GTCAAGGGAA 
ACATTTGAAA GCCTCATGTA CGGACACAAG ATGCTGGAGT ATGGTGAGTA CAAGGGCCAC 
CTGTACGGAA CCAGTGTGAA CGCTGTACAC GCCGTCCTTG ATGAAGGCAA GATCTGCATC 
ATGGATCTAG AGCCTCAGGA TATTCAGTCG GCTCGAACCC GTGACTTGAA GCCCTACGTT 
ATATTTATTA AGCCACCTAA CACGAGCTCC ATGAGACACT CTCGGAAAAA CGCTAAGATC 
ACCACTGACT ACTATGTGGA CATGAAGTTC AAGGATGAAG ACCTACAAGA GATGGAGGAG 
TTAGCCCAGA AAATGGAGAG TCAGTTTGGC CAGTTTTTTG ATCACGTGAT TGTGAATGAC 
AACTTGCAAG ATGCATGTGG CCAGCTGTTG TCCGCCATAC AGAAGGCTCA GGAAGAACTG 
CAGTGGGTGC CAGAGGCATG GGTTTCCCCT GACACTGAAT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mpp4 ( NM_145143.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_660125.2 Mus musculus membrane protein, palmitoylated 4 (MAGUK p55 subfamily member 4) (Mpp4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu11346
Clone ID Related Accession (Same CDS sequence)	NM_145143.3
Accession Version	NM_145143.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1908bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Mus musculus(house mouse)
Product	MAGUK p55 subfamily member 4 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BI737565.1 and BC061694.1. On Dec 11, 2007 this sequence version replaced NM_145143.2. Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC061694.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164136, SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGACAGT CTGACAGAGG AGCAGAGCTG ACCAATGAGG ACAGAGCTCT TCCCACGCCA 
CCTGATCCTG AGAATGGTCT CTCTGGGATC CTGAGGCTCC TACTGCAGGA ACTGAGCCTT 
TTCTACAGCC GAGATGTGAA TGGTCTGTGT CTCTTATATG ACCTCCTCCA TTCACCATGG 
CTGCAGGCTC TGCTCAAGGT CTACGACTGC CTCCAGAGGT TTAAAGAGAA GAAGCTAGTT 
CCTGATACAA CTCATGCACA GATCTTAGCC TGTGAGGTCT TGGAGCTGTT ACCCAAAGCT 
TCCGGTTCCC CCGAGATCCA AGAGCTAAGA CAAGTCCTTC AGGCTCCTCA CTGCAAGGCC 
TTGCTGAGTG CCCATGACAC AGTGGCTCAG AAAGACTTTG AACCCCTTCT CCCCCCACTG 
CCAGACAATA TCCCCGACAG TGAAGAAGCA ATGAGGATTG TTTGTCTGGT GAAAAACCAA 
CAGCCCCTGG GAGCAACCAT CAAGCGTCAT GAGATAACAG GGGATATCCT GGTGGCCAGG 
GTCATCCATG GCGGGCTGGT GGAGAGGAGC GGGTTGCTGT ATGCTGGAGA CAAACTGGTG 
GAAGTGAATG GAGTTTCAGT AGAGGGCCTG GACCCTGAGC AAGTGATCCA TATTTTGGCT 
ATGTCTTGTG GTACGATCAT GTTCAAGGTG ATTCCGGTCT CTGCCCCGCC TGTGAGCAGC 
CAGAAGATGG TTTATGTTCG CGCCATGATT GATTACTGGC CCCAAGAGGA TCCCGACATC 
CCCTGCATGG ATGCTGGGCT GCCTTTCCTG AAAGGGGACA TTCTACAGAT TGTGGACCAG 
AATGATGCCC TCTGGTGGCA GGCCCGGAAA ATCTCAGACC TCACCATCTG TGCTGGGCTA 
ATCCCTTCTA ACCACCTTCT GAAGAGGAAG CAGCGGGAGT TCTGGTGGTC TCAGCCGTAC 
CAACCTCACA CCTGCCTCAA ATCCACCCGA GCACTGTCTA TGGAGGAAGA AGACAGCATG 
AAGATTGATG AGAAGTGTGT GGAAGCAGAT GAAGAAACTT TTGAATCTGA GGAACTCGCA 
GAAGCTAAGG ACGAATTTGT CGGCGATGGG CAGAAGTTCT TTATCGCTGG GTTCCGCCGC 
AGCATGCGCC TCTGTCGCAG GAAGTCTCAC TTCAGCCAGC TGCATGCCAG CCTGTGCTGC 
AGCTGCAGCT GCTACAGTGC CGTGGGCGCT CCTTACGAGG AGGTGGTGAG GTACCAGCGG 
CAGCCGGCGG ACAAACACCG CCTCATAGTG CTTGTGGGAC CTTCTGGCGT TGGAGTCAAT 
GAACTGAGAA GACAGCTCAT TGGATGTAAC CCCAGCTGTT TTCAAAGTGC TGTGCCACAT 
ACCACCCGTT TCCCAAAGAG TTATGAAATG GATGGACGTG AGTATCACTA CGTGTCAAGG 
GAAACATTTG AAAGCCTCAT GTACGGACAC AAGATGCTGG AGTATGGTGA GTACAAGGGC 
CACCTGTACG GAACCAGTGT GAACGCTGTA CACGCCGTCC TTGATGAAGG CAAGATCTGC 
ATCATGGATC TAGAGCCTCA GGATATTCAG TCGGCTCGAA CCCGTGACTT GAAGCCCTAC 
GTTATATTTA TTAAGCCACC TAACACGAGC TCCATGAGAC ACTCTCGGAA AAACGCTAAG 
ATCACCACTG ACTACTATGT GGACATGAAG TTCAAGGATG AAGACCTACA AGAGATGGAG 
GAGTTAGCCC AGAAAATGGA GAGTCAGTTT GGCCAGTTTT TTGATCACGT GATTGTGAAT 
GACAACTTGC AAGATGCATG TGGCCAGCTG TTGTCCGCCA TACAGAAGGC TCAGGAAGAA 
CTGCAGTGGG TGCCAGAGGC ATGGGTTTCC CCTGACACTG AATCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_660125.2
CDS	120..2027
Translation	MRQSDRGAELTNEDRALPTPPDPENGLSGILRLLLQELSLFYSRDVNGLCLLYDLLHSPWLQALLKVYDCLQRFKEKKLVPDTTHAQILACEVLELLPKASGSPEIQELRQVLQAPHCKALLSAHDTVAQKDFEPLLPPLPDNIPDSEEAMRIVCLVKNQQPLGATIKRHEITGDILVARVIHGGLVERSGLLYAGDKLVEVNGVSVEGLDPEQVIHILAMSCGTIMFKVIPVSAPPVSSQKMVYVRAMIDYWPQEDPDIPCMDAGLPFLKGDILQIVDQNDALWWQARKISDLTICAGLIPSNHLLKRKQREFWWSQPYQPHTCLKSTRALSMEEEDSMKIDEKCVEADEETFESEELAEAKDEFVGDGQKFFIAGFRRSMRLCRRKSHFSQLHASLCCSCSCYSAVGAPYEEVVRYQRQPADKHRLIVLVGPSGVGVNELRRQLIGCNPSCFQSAVPHTTRFPKSYEMDGREYHYVSRETFESLMYGHKMLEYGEYKGHLYGTSVNAVHAVLDEGKICIMDLEPQDIQSARTRDLKPYVIFIKPPNTSSMRHSRKNAKITTDYYVDMKFKDEDLQEMEELAQKMESQFGQFFDHVIVNDNLQDACGQLLSAIQKAQEELQWVPEAWVSPDTES

Target ORF information:

RefSeq Version	NM_145143.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus membrane protein, palmitoylated 4 (MAGUK p55 subfamily member 4) (Mpp4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145143.3

ORF Insert Sequence:

ATGAGACAGT CTGACAGAGG AGCAGAGCTG ACCAATGAGG ACAGAGCTCT TCCCACGCCA 
CCTGATCCTG AGAATGGTCT CTCTGGGATC CTGAGGCTCC TACTGCAGGA ACTGAGCCTT 
TTCTACAGCC GAGATGTGAA TGGTCTGTGT CTCTTATATG ACCTCCTCCA TTCACCATGG 
CTGCAGGCTC TGCTCAAGGT CTACGACTGC CTCCAGAGGT TTAAAGAGAA GAAGCTAGTT 
CCTGATACAA CTCATGCACA GATCTTAGCC TGTGAGGTCT TGGAGCTGTT ACCCAAAGCT 
TCCGGTTCCC CCGAGATCCA AGAGCTAAGA CAAGTCCTTC AGGCTCCTCA CTGCAAGGCC 
TTGCTGAGTG CCCATGACAC AGTGGCTCAG AAAGACTTTG AACCCCTTCT CCCCCCACTG 
CCAGACAATA TCCCCGACAG TGAAGAAGCA ATGAGGATTG TTTGTCTGGT GAAAAACCAA 
CAGCCCCTGG GAGCAACCAT CAAGCGTCAT GAGATAACAG GGGATATCCT GGTGGCCAGG 
GTCATCCATG GCGGGCTGGT GGAGAGGAGC GGGTTGCTGT ATGCTGGAGA CAAACTGGTG 
GAAGTGAATG GAGTTTCAGT AGAGGGCCTG GACCCTGAGC AAGTGATCCA TATTTTGGCT 
ATGTCTTGTG GTACGATCAT GTTCAAGGTG ATTCCGGTCT CTGCCCCGCC TGTGAGCAGC 
CAGAAGATGG TTTATGTTCG CGCCATGATT GATTACTGGC CCCAAGAGGA TCCCGACATC 
CCCTGCATGG ATGCTGGGCT GCCTTTCCTG AAAGGGGACA TTCTACAGAT TGTGGACCAG 
AATGATGCCC TCTGGTGGCA GGCCCGGAAA ATCTCAGACC TCACCATCTG TGCTGGGCTA 
ATCCCTTCTA ACCACCTTCT GAAGAGGAAG CAGCGGGAGT TCTGGTGGTC TCAGCCGTAC 
CAACCTCACA CCTGCCTCAA ATCCACCCGA GCACTGTCTA TGGAGGAAGA AGACAGCATG 
AAGATTGATG AGAAGTGTGT GGAAGCAGAT GAAGAAACTT TTGAATCTGA GGAACTCGCA 
GAAGCTAAGG ACGAATTTGT CGGCGATGGG CAGAAGTTCT TTATCGCTGG GTTCCGCCGC 
AGCATGCGCC TCTGTCGCAG GAAGTCTCAC TTCAGCCAGC TGCATGCCAG CCTGTGCTGC 
AGCTGCAGCT GCTACAGTGC CGTGGGCGCT CCTTACGAGG AGGTGGTGAG GTACCAGCGG 
CAGCCGGCGG ACAAACACCG CCTCATAGTG CTTGTGGGAC CTTCTGGCGT TGGAGTCAAT 
GAACTGAGAA GACAGCTCAT TGGATGTAAC CCCAGCTGTT TTCAAAGTGC TGTGCCACAT 
ACCACCCGTT TCCCAAAGAG TTATGAAATG GATGGACGTG AGTATCACTA CGTGTCAAGG 
GAAACATTTG AAAGCCTCAT GTACGGACAC AAGATGCTGG AGTATGGTGA GTACAAGGGC 
CACCTGTACG GAACCAGTGT GAACGCTGTA CACGCCGTCC TTGATGAAGG CAAGATCTGC 
ATCATGGATC TAGAGCCTCA GGATATTCAG TCGGCTCGAA CCCGTGACTT GAAGCCCTAC 
GTTATATTTA TTAAGCCACC TAACACGAGC TCCATGAGAC ACTCTCGGAA AAACGCTAAG 
ATCACCACTG ACTACTATGT GGACATGAAG TTCAAGGATG AAGACCTACA AGAGATGGAG 
GAGTTAGCCC AGAAAATGGA GAGTCAGTTT GGCCAGTTTT TTGATCACGT GATTGTGAAT 
GACAACTTGC AAGATGCATG TGGCCAGCTG TTGTCCGCCA TACAGAAGGC TCAGGAAGAA 
CTGCAGTGGG TGCCAGAGGC ATGGGTTTCC CCTGACACTG AATCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Motor phenotypes and molecular networks associated with germline deficiency of Ciz1.
Experimental neurology283(Pt A)110-20(2016 Sep)
Xiao J,Vemula SR,Xue Y,Khan MM,Kuruvilla KP,Marquez-Lona EM,Cobb MR,LeDoux MS

The first murine zygotic transcription is promiscuous and uncoupled from splicing and 3' processing.
The EMBO journal34(11)1523-37(2015 Jun)
Abe K,Yamamoto R,Franke V,Cao M,Suzuki Y,Suzuki MG,Vlahovicek K,Svoboda P,Schultz RM,Aoki F

PSD95beta regulates plasma membrane Ca2+ pump localization at the photoreceptor synapse.
Molecular and cellular neurosciences41(2)156-65(2009 Jun)
Aartsen WM,Arsanto JP,Chauvin JP,Vos RM,Versteeg I,Cardozo BN,Bivic AL,Wijnholds J

Mpp4 is required for proper localization of plasma membrane calcium ATPases and maintenance of calcium homeostasis at the rod photoreceptor synaptic terminals.
Human molecular genetics16(9)1017-29(2007 May)
Yang J,Pawlyk B,Wen XH,Adamian M,Soloviev M,Michaud N,Zhao Y,Sandberg MA,Makino CL,Li T

Cracking the egg: molecular dynamics and evolutionary aspects of the transition from the fully grown oocyte to embryo.
Genes & development20(19)2713-27(2006 Oct)
Evsikov AV,Graber JH,Brockman JM,Hampl A,Holbrook AE,Singh P,Eppig JJ,Solter D,Knowles BB

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Mpp4 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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