Aph1a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Aph1a
Entrez Gene ID	226548
Full Name	aph1 homolog A, gamma secretase subunit
Synonyms	6530402N02Rik,APH-1a
General protein information	Preferred Names aph1 homolog A, gamma secretase subunit Names gamma-secretase subunit APH-1A anterior pharynx defective 1a homolog aph-1alpha
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3\|3 F2.1
Summary	This gene encodes a subunit of the gamma-secretase complex, which is localized to the endoplasmic reticulum and golgi apparatus. Gamma-secretase is a multi-protein enzyme that catalyzes intramembraneous proteolysis of type I transmembrane proteins and is essential for many signaling pathways, including the Notch signaling pathway. Studies suggest that the protein encoded by this locus binds directly to substrates of the gamma-secretase complex, including the beta-amyloid precursor protein which is associated with Alzheimer disease progression. This gene is required for normal embryonic development and survival, and disruption is associated with defects in the yolk sack angiogenesis, neural tube formation, and somitogenesis. A pseudogene of this gene is located on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013].

mRNA and Protein(s)

mRNA	Protein	Name
NM_146134.2	NP_666246.1	gamma-secretase subunit APH-1A isoform 2
NM_146104.3	NP_666216.1	gamma-secretase subunit APH-1A isoform 1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Aph1a	NP_666246.1
Rattus norvegicus (Norway rat)	Aph1a	NP_001014277.1
Drosophila melanogaster (fruit fly)	aph-1	NP_001259963.1
Pan troglodytes (chimpanzee)	APH1A	XP_513772.1
Bos taurus (cattle)	APH1A	NP_001029842.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009816	XP_318923.2
Gallus gallus (chicken)	LOC431243	XP_428797.3
Danio rerio (zebrafish)	aph1b	NP_956409.1
Oryza sativa (rice)	Os02g0272600	NP_001046527.1
	APH1A	XP_001100088.1
Caenorhabditis elegans (roundworm)	aph-1	NP_492469.1
Homo sapiens (human)	APH1A	NP_001071096.1
Canis lupus familiaris (dog)	APH1	XP_003639673.1
Arabidopsis thaliana (thale cress)	AT2G31440	NP_565724.1
Xenopus tropicalis (tropical clawed frog)	aph1a	NP_001015843.1

Gene Ontology
Bibliography

Related articles in PubMed

Inactivation of ?-secretases leads to accumulation of substrates and non-Alzheimer neurodegeneration.
Acx H, Serneels L, Radaelli E, Muyldermans S, Vincke C, Pepermans E, M?ller U, Ch?vez-Guti?rrez L, De Strooper B
EMBO molecular medicine9(8)1088-1099(2017 Aug)

Posttranslational proteolytic processing of Leda-1/Pianp involves cleavage by MMPs, ADAM10/17 and gamma-secretase.
Biswas S, Adrian M, Weber J, Evdokimov K, Winkler M, G?raud C
Biochemical and biophysical research communications477(4)661-666(2016 09)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Prostaglandin I2 accumulation in neuronal cells activates PKA/CREB and JNK/c-Jun signaling pathways by phosphorylation, which results in APH-1alpha/1beta expression.
Title: Prostaglandin I2 upregulates the expression of anterior pharynx-defective-1? and anterior pharynx-defective-1? in amyloid precursor protein/presenilin 1 transgenic mice.

Thus, Leda-1/Pianp is constitutively processed by proprotein convertases, sheddases including MMPs and ADAM10/17 and intramembrane protease gamma-secretase.
Title: Posttranslational proteolytic processing of Leda-1/Pianp involves cleavage by MMPs, ADAM10/17 and gamma-secretase.

Abeta oligomers in the cerebrospinal fluid (CSF) further promoted the expression of APH-1alpha/-1beta (by >2.5-fold), which enhances the gamma-cleavage of APP and Abeta deposition during AD progression
Title: By suppressing the expression of anterior pharynx-defective-1? and -1? and inhibiting the aggregation of ?-amyloid protein, magnesium ions inhibit the cognitive decline of amyloid precursor protein/presenilin 1 transgenic mice.

ficiency of beta-arrestin1 or inhibition of binding of beta-arrestin1 with APH-1 by small peptides reduced Abeta production without affecting Notch processing
Title: ?-arrestin1 regulates ?-secretase complex assembly and modulates amyloid-? pathology.

Pen-2, as well as nicastrin and Aph-1alpha, is dispensable for presenilin endoproteolysis
Title: Pen-2 is dispensable for endoproteolysis of presenilin 1, and nicastrin-Aph subcomplex is important for both ?-secretase assembly and substrate recruitment.

The following Aph1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Aph1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu12223	NM_146104.3 Latest version!	Mus musculus aph1 homolog A, gamma secretase subunit (Aph1a), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu12252	NM_146134.2 Latest version!	Mus musculus aph1 homolog A, gamma secretase subunit (Aph1a), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Aph1a ( NM_146104.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_666216.1 Mus musculus aph1 homolog A, gamma secretase subunit (Aph1a), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu12223
Clone ID Related Accession (Same CDS sequence)	NM_146104.3
Accession Version	NM_146104.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-26
Organism	Mus musculus(house mouse)
Product	gamma-secretase subunit APH-1A isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY264531.1, AK148400.1 and AC092855.39. On Jan 30, 2013 this sequence version replaced NM_146104.2. Transcript Variant: This variant (1) differs in the 3' coding region and 3' UTR, compared to variant 2. The resulting isoform (1, also known as isoform S) is shorter and has a distinct C-terminus, compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data.The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC012406.1, AK148400.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGGCTG CTGTGTTTTT CGGATGCACC TTCGTCGCGT TCGGCCCAGC CTTCTCCCTT 
TTCCTGATCA CTGTAGCTGG AGACCCACTT CGGGTTATCA TCCTGGTGGC GGGAGCCTTT 
TTCTGGCTGG TCTCCCTGCT CTTGGCTTCT GTGGTCTGGT TCATCTTGGT CCATGTGACA 
GACCGATCAG ATGCACGGCT CCAGTATGGC CTCCTGATTT TTGGTGCTGC TGTCTCTGTC 
CTTCTACAGG AAGTGTTCCG TTTTGCTTAC TACAAGCTCC TTAAGAAGGC AGATGAGGGC 
TTAGCATCAC TGAGTGAGGA CGGAAGATCA CCCATCTCCA TCCGACAGAT GGCCTATGTT 
TCTGGTCTGT CCTTCGGTAT CATCAGTGGT GTCTTCTCTG TTATCAATAT TTTGGCTGAT 
GCACTTGGGC CAGGTGTGGT TGGGATCCAT GGAGACTCAC CCTATTACTT CCTGACTTCA 
GCCTTTCTGA CAGCAGCCAT TATCCTGCTC CACACCTTTT GGGGAGTTGT GTTCTTTGAT 
GCCTGTGAGA GGAGACGGTA CTGGGCTTTG GGCCTGGTAG TTGGGAGTCA CCTTCTGACA 
TCGGGACTGA CATTCCTGAA CCCCTGGTAT GAGGCTAGCC TGCTGCCCAT CTATGCAGTC 
ACCGTTTCCA TGGGGCTCTG GGCGTTCATC ACAGCCGGAG GCTCCCTCCG AAGTATCCAG 
CGCAGCCTTT CGTGTAAGGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_666216.1
CDS	251..994
Translation	MGAAVFFGCTFVAFGPAFSLFLITVAGDPLRVIILVAGAFFWLVSLLLASVVWFILVHVTDRSDARLQYGLLIFGAAVSVLLQEVFRFAYYKLLKKADEGLASLSEDGRSPISIRQMAYVSGLSFGIISGVFSVINILADALGPGVVGIHGDSPYYFLTSAFLTAAIILLHTFWGVVFFDACERRRYWALGLVVGSHLLTSGLTFLNPWYEASLLPIYAVTVSMGLWAFITAGGSLRSIQRSLSCKD

Target ORF information:

RefSeq Version	NM_146104.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus aph1 homolog A, gamma secretase subunit (Aph1a), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_146104.3

ORF Insert Sequence:

ATGGGGGCTG CTGTGTTTTT CGGATGCACC TTCGTCGCGT TCGGCCCAGC CTTCTCCCTT 
TTCCTGATCA CTGTAGCTGG AGACCCACTT CGGGTTATCA TCCTGGTGGC GGGAGCCTTT 
TTCTGGCTGG TCTCCCTGCT CTTGGCTTCT GTGGTCTGGT TCATCTTGGT CCATGTGACA 
GACCGATCAG ATGCACGGCT CCAGTATGGC CTCCTGATTT TTGGTGCTGC TGTCTCTGTC 
CTTCTACAGG AAGTGTTCCG TTTTGCTTAC TACAAGCTCC TTAAGAAGGC AGATGAGGGC 
TTAGCATCAC TGAGTGAGGA CGGAAGATCA CCCATCTCCA TCCGACAGAT GGCCTATGTT 
TCTGGTCTGT CCTTCGGTAT CATCAGTGGT GTCTTCTCTG TTATCAATAT TTTGGCTGAT 
GCACTTGGGC CAGGTGTGGT TGGGATCCAT GGAGACTCAC CCTATTACTT CCTGACTTCA 
GCCTTTCTGA CAGCAGCCAT TATCCTGCTC CACACCTTTT GGGGAGTTGT GTTCTTTGAT 
GCCTGTGAGA GGAGACGGTA CTGGGCTTTG GGCCTGGTAG TTGGGAGTCA CCTTCTGACA 
TCGGGACTGA CATTCCTGAA CCCCTGGTAT GAGGCTAGCC TGCTGCCCAT CTATGCAGTC 
ACCGTTTCCA TGGGGCTCTG GGCGTTCATC ACAGCCGGAG GCTCCCTCCG AAGTATCCAG 
CGCAGCCTTT CGTGTAAGGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Aph1a ( NM_146134.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_666246.1 Mus musculus aph1 homolog A, gamma secretase subunit (Aph1a), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu12252
Clone ID Related Accession (Same CDS sequence)	NM_146134.2
Accession Version	NM_146134.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 798bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-26
Organism	Mus musculus(house mouse)
Product	gamma-secretase subunit APH-1A isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY264531.1, AK148400.1, CX207467.1 and AC092855.39. On Jan 30, 2013 this sequence version replaced NM_146134.1. Transcript Variant: This variant (2) encodes the longer isoform (2, also known as isoform L). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC024111.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGGCTG CTGTGTTTTT CGGATGCACC TTCGTCGCGT TCGGCCCAGC CTTCTCCCTT 
TTCCTGATCA CTGTAGCTGG AGACCCACTT CGGGTTATCA TCCTGGTGGC GGGAGCCTTT 
TTCTGGCTGG TCTCCCTGCT CTTGGCTTCT GTGGTCTGGT TCATCTTGGT CCATGTGACA 
GACCGATCAG ATGCACGGCT CCAGTATGGC CTCCTGATTT TTGGTGCTGC TGTCTCTGTC 
CTTCTACAGG AAGTGTTCCG TTTTGCTTAC TACAAGCTCC TTAAGAAGGC AGATGAGGGC 
TTAGCATCAC TGAGTGAGGA CGGAAGATCA CCCATCTCCA TCCGACAGAT GGCCTATGTT 
TCTGGTCTGT CCTTCGGTAT CATCAGTGGT GTCTTCTCTG TTATCAATAT TTTGGCTGAT 
GCACTTGGGC CAGGTGTGGT TGGGATCCAT GGAGACTCAC CCTATTACTT CCTGACTTCA 
GCCTTTCTGA CAGCAGCCAT TATCCTGCTC CACACCTTTT GGGGAGTTGT GTTCTTTGAT 
GCCTGTGAGA GGAGACGGTA CTGGGCTTTG GGCCTGGTAG TTGGGAGTCA CCTTCTGACA 
TCGGGACTGA CATTCCTGAA CCCCTGGTAT GAGGCTAGCC TGCTGCCCAT CTATGCAGTC 
ACCGTTTCCA TGGGGCTCTG GGCGTTCATC ACAGCCGGAG GCTCCCTCCG AAGTATCCAG 
CGCAGCCTTT CGTGCCGCCG GCAGGAGGAC AGTCGGGTGA TGGTGTACTC TGCCCTGCGC 
ATCCCACCCG AGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_666246.1
CDS	251..1048
Translation	MGAAVFFGCTFVAFGPAFSLFLITVAGDPLRVIILVAGAFFWLVSLLLASVVWFILVHVTDRSDARLQYGLLIFGAAVSVLLQEVFRFAYYKLLKKADEGLASLSEDGRSPISIRQMAYVSGLSFGIISGVFSVINILADALGPGVVGIHGDSPYYFLTSAFLTAAIILLHTFWGVVFFDACERRRYWALGLVVGSHLLTSGLTFLNPWYEASLLPIYAVTVSMGLWAFITAGGSLRSIQRSLSCRRQEDSRVMVYSALRIPPED

Target ORF information:

RefSeq Version	NM_146134.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus aph1 homolog A, gamma secretase subunit (Aph1a), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_146134.2

ORF Insert Sequence:

ATGGGGGCTG CTGTGTTTTT CGGATGCACC TTCGTCGCGT TCGGCCCAGC CTTCTCCCTT 
TTCCTGATCA CTGTAGCTGG AGACCCACTT CGGGTTATCA TCCTGGTGGC GGGAGCCTTT 
TTCTGGCTGG TCTCCCTGCT CTTGGCTTCT GTGGTCTGGT TCATCTTGGT CCATGTGACA 
GACCGATCAG ATGCACGGCT CCAGTATGGC CTCCTGATTT TTGGTGCTGC TGTCTCTGTC 
CTTCTACAGG AAGTGTTCCG TTTTGCTTAC TACAAGCTCC TTAAGAAGGC AGATGAGGGC 
TTAGCATCAC TGAGTGAGGA CGGAAGATCA CCCATCTCCA TCCGACAGAT GGCCTATGTT 
TCTGGTCTGT CCTTCGGTAT CATCAGTGGT GTCTTCTCTG TTATCAATAT TTTGGCTGAT 
GCACTTGGGC CAGGTGTGGT TGGGATCCAT GGAGACTCAC CCTATTACTT CCTGACTTCA 
GCCTTTCTGA CAGCAGCCAT TATCCTGCTC CACACCTTTT GGGGAGTTGT GTTCTTTGAT 
GCCTGTGAGA GGAGACGGTA CTGGGCTTTG GGCCTGGTAG TTGGGAGTCA CCTTCTGACA 
TCGGGACTGA CATTCCTGAA CCCCTGGTAT GAGGCTAGCC TGCTGCCCAT CTATGCAGTC 
ACCGTTTCCA TGGGGCTCTG GGCGTTCATC ACAGCCGGAG GCTCCCTCCG AAGTATCCAG 
CGCAGCCTTT CGTGCCGCCG GCAGGAGGAC AGTCGGGTGA TGGTGTACTC TGCCCTGCGC 
ATCCCACCCG AGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Inactivation of ?-secretases leads to accumulation of substrates and non-Alzheimer neurodegeneration.
EMBO molecular medicine9(8)1088-1099(2017 Aug)
Acx H,Serneels L,Radaelli E,Muyldermans S,Vincke C,Pepermans E,M?ller U,Ch?vez-Guti?rrez L,De Strooper B

Posttranslational proteolytic processing of Leda-1/Pianp involves cleavage by MMPs, ADAM10/17 and gamma-secretase.
Biochemical and biophysical research communications477(4)661-666(2016 09)
Biswas S,Adrian M,Weber J,Evdokimov K,Winkler M,G?raud C

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Aph1a cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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