Igfn1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Igfn1
Entrez Gene ID	226438
Full Name	immunoglobulin-like and fibronectin type III domain containing 1
Synonyms	9830123M21Rik
General protein information	Preferred Names immunoglobulin-like and fibronectin type III domain containing 1 Names immunoglobulin-like and fibronectin type III domain-containing protein 1 eEF1A2 binding protein immunoglobulin-like and fibronectin type 3 domain containing 1 protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1\|1 E4

mRNA and Protein(s)

mRNA	Protein	Name
XM_017319938.1	XP_017175427.1	immunoglobulin-like and fibronectin type III domain-containing protein 1 isoform X1
XM_017319940.1	XP_017175429.1	immunoglobulin-like and fibronectin type III domain-containing protein 1 isoform X1
NM_177642.3	NP_808310.2	immunoglobulin-like and fibronectin type III domain-containing protein 1
XM_017319947.1	XP_017175436.1	immunoglobulin-like and fibronectin type III domain-containing protein 1 isoform X5
XM_017319937.1	XP_017175426.1	immunoglobulin-like and fibronectin type III domain-containing protein 1 isoform X1
XM_017319943.1	XP_017175432.1	immunoglobulin-like and fibronectin type III domain-containing protein 1 isoform X2
XM_006529440.3	XP_006529503.1	immunoglobulin-like and fibronectin type III domain-containing protein 1 isoform X3
XM_006529442.3	XP_006529505.1	immunoglobulin-like and fibronectin type III domain-containing protein 1 isoform X4

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Igfn1	NP_808310.2
Danio rerio (zebrafish)	LOC100331959	XP_005168832.1
Pan troglodytes (chimpanzee)	IGFN1	XP_514089.3
Rattus norvegicus (Norway rat)	Igfn1	XP_006249965.1
Homo sapiens (human)	IGFN1	NP_001158058.1
Danio rerio (zebrafish)	LOC100331898	XP_002662567.3

Gene Ontology
Bibliography

Related articles in PubMed

IGFN1_v1 is required for myoblast fusion and differentiation.
Li X, Baker J, Cracknell T, Haynes AR, Blanco G
PloS one12(6)e0180217(2017)

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Thompson CL, Ng L, Menon V, Martinez S, Lee CK, Glattfelder K, Sunkin SM, Henry A, Lau C, Dang C, Garcia-Lopez R, Martinez-Ferre A, Pombero A, Rubenstein JLR, Wakeman WB, Hohmann J, Dee N, Sodt AJ, Young R, Smith K, Nguyen TN, Kidney J, Kuan L, Jeromin A, Kaykas A, Miller J, Page D, Orta G, Bernard A, Riley Z, Smith S, Wohnoutka P, Hawrylycz MJ, Puelles L, Jones AR
Neuron83(2)309-323(2014 Jul)

Identification of a Z-band associated protein complex involving KY, FLNC and IGFN1.
Baker J, Riley G, Romero MR, Haynes AR, Hilton H, Simon M, Hancock J, Tateossian H, Ripoll VM, Blanco G
Experimental cell research316(11)1856-70(2010 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Expression of IGFN1_v1 partially rescued fusion and myotube morphology in the Igfn1 exon 13 knock-out cell line, indicating a role for this variant in myoblast fusion and differentiation
Title: IGFN1_v1 is required for myoblast fusion and differentiation.

KY, IGFN1 and FLNC are part of a Z-band associated protein complex likely to provide structural support to the skeletal muscle sarcomere.
Title: Identification of a Z-band associated protein complex involving KY, FLNC and IGFN1.

The following Igfn1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Igfn1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu77090	XM_017319947.1 Latest version!	Mus musculus immunoglobulin-like and fibronectin type III domain containing 1 (Igfn1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu28133	XM_006529442.3 Latest version!	Mus musculus immunoglobulin-like and fibronectin type III domain containing 1 (Igfn1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu77089	XM_017319943.1 Latest version!	Mus musculus immunoglobulin-like and fibronectin type III domain containing 1 (Igfn1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMu28131	XM_006529440.3 Latest version!	Mus musculus immunoglobulin-like and fibronectin type III domain containing 1 (Igfn1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OMu06590	NM_177642.3 Latest version!	Mus musculus immunoglobulin-like and fibronectin type III domain containing 1 (Igfn1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu77088	XM_017319938.1 Latest version!	Mus musculus immunoglobulin-like and fibronectin type III domain containing 1 (Igfn1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu77088	XM_017319940.1 Latest version!	Mus musculus immunoglobulin-like and fibronectin type III domain containing 1 (Igfn1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu77088	XM_017319937.1 Latest version!	Mus musculus immunoglobulin-like and fibronectin type III domain containing 1 (Igfn1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Igfn1 ( XM_017319947.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175436.1 Mus musculus immunoglobulin-like and fibronectin type III domain containing 1 (Igfn1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OMu77090
Clone ID Related Accession (Same CDS sequence)	XM_017319947.1
Accession Version	XM_017319947.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	immunoglobulin-like and fibronectin type III domain-containing protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAACGA GGTGGTGGGC GGCGACCACA AGGGCATCCA ACTGGCTCTG GCAGATGGCT 
ACACATGGCT GTGCCTCCCC AGCGTGTGCA GGAAGGACAG TGGCCGACAA GCCTCAGCCC 
CCACAAGGAC CCCTGGAGGT TCAGGATTGC CACAGAGCAG GTGTCTGCCT CCGCTGGCTA 
CCCCCGAGGG ACAACGGGGG CCAGGTCATA CAACACTATG TGGTGGAGAG GCGACAGGCT 
GGAAGGAGCA CTTGGTTGAA GGTGGGCGAG CCCCCATCAG ACAGTACCAG CTTCACCGAC 
ACCAGCGTGG AGCAGGGAAA GAAATATGCC TTCCGTGTGC GGGCTGTGAC CTCAGTGGGA 
GCCGGAGATG CCCTGGAGTC AGAGGAGGTG TTGGTGGCTC CTGAGGCTCT CCCGGGGCCC 
CCTTCTGCCC CAGCCATCCT GTCAGCCTCC AGTCAGAGCA TCACTCTCAC ATGGGGGGCA 
CCACAGGGCC CTGGCAGTAA CCACATCCTG GGCTACCTGA TCGAGAAGCA CAAGAAGGGG 
AGCAACACCT GGATGGCTGT GAATGAGCAG CCCGTGTCTG AAAGAAGGTA CACTGTGGTG 
GGTCTGCGAC AGGGCTGCCA GTATGAGTTC CGGGTCACGG CCGTGACTCT GTCGGGCCCT 
GGAGATCCTG GGCCTCCATC GGATGCTGTC TTTGCTAGAG ACCCTATGAG ACCCCCAGGG 
CCTGTGCGGG ATCTACAGGT TACAGACACA TCAAACACTA GCATCACCGT GAGCTGGATG 
GTGCCGGATG CCCGGGATGC TGATGAAGCC CAGGGATATA TTGTGGAGTT GTGTGGTTCA 
GACAGCCATC AATGGAGCCC ATGTCACGTG GGAACTGTGC CGGGCACCAC CTTCACAGCC 
AAGGGGCTTC GGCCGCAAGA AGGCTACTTT GTGCGGGTGA CAGCAGTTAA TGATGGGGGC 
CGGAGCCAGG CCACTTCCCT GGACACATTG GTTCATGCCA TGCCGGCCAC TGTCTGTCCC 
AGGTTCCTCA TGGACTCCGG CACAAAGGAT ACCCTGATGG TCAGGGTTGG GGACAGTATT 
CGCGTTCCTC TCTCCTTTGA AGCTGCCCCC ATGCCTGAGG TGACCTGGCT GAAGGACGGC 
TTGCCCTTGC CCAAAAGAAG TGTGACCACT GTGAAGGCTG GCCTCACGCA GCTTCTGATC 
CCTGCAGCTA GCCTCTCGGA CTGTGGCCGA TACACCGTGA TGCTGAGGAA CCTACAGGGG 
AAGGAAGCTA CCCACAGCTT CTTCATCAAT GTAGCAGCAT GCCCACAGGC ACCAGGTTCT 
ATCTACCTAC AGGAGAATGT GCCTGGGACA GTGACGGTCC AGTGGGAACC CTCTCCGGAT 
GAGGCTCAGG GCATCCCCTT GCATTACACA GTGCTGATGC GGTCCTCATC TCACCGATCC 
TGGCACGAGG TGGCCGACCA TGTCCGTACC AACCGCTTTA CCCTCCTAGG GGTCCTCCCT 
GGCCATGAGT ACCACTTTCG GGTGCTGGCC AAGAATGAGC TGGGGGTCAG CAAACCTTCA 
GACACCAGCC AACCCTGGTG TATCCCCAGG CAGAGAGACA GGTTCACAGT GAAGTCTCCG 
ACATACCAGG ATCCTGACCT GAGCCAGAAG CCCCGCTTCC TGGTGGGTCT GCGGGCTCAC 
CTTCTGCCCC AGGGCTGTGA GTGCCGCATG ACGTGTGCCG TGCAGGGCTC ACCCCAGCCT 
CATGTCACCT GGTTCAAGAA TGACCAAAGC TTGGACAGAA ACCCTGCGGT GTACAGTACT 
GACTTGCTGG GTGTGTGCTC CCTCGTCATC CCCAGCGTGT CCCTTAAGGA CAGTGGTGAA 
TACAAGGCTG TGGCCAAGAA CCCACTGGGA CAAGCTGTCA GCACAGCCAC CCTCATTGTC 
ACAGAATACG ACTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175436.1
CDS	88..2025
Translation	MGTRWWAATTRASNWLWQMATHGCASPACAGRTVADKPQPPQGPLEVQDCHRAGVCLRWLPPRDNGGQVIQHYVVERRQAGRSTWLKVGEPPSDSTSFTDTSVEQGKKYAFRVRAVTSVGAGDALESEEVLVAPEALPGPPSAPAILSASSQSITLTWGAPQGPGSNHILGYLIEKHKKGSNTWMAVNEQPVSERRYTVVGLRQGCQYEFRVTAVTLSGPGDPGPPSDAVFARDPMRPPGPVRDLQVTDTSNTSITVSWMVPDARDADEAQGYIVELCGSDSHQWSPCHVGTVPGTTFTAKGLRPQEGYFVRVTAVNDGGRSQATSLDTLVHAMPATVCPRFLMDSGTKDTLMVRVGDSIRVPLSFEAAPMPEVTWLKDGLPLPKRSVTTVKAGLTQLLIPAASLSDCGRYTVMLRNLQGKEATHSFFINVAACPQAPGSIYLQENVPGTVTVQWEPSPDEAQGIPLHYTVLMRSSSHRSWHEVADHVRTNRFTLLGVLPGHEYHFRVLAKNELGVSKPSDTSQPWCIPRQRDRFTVKSPTYQDPDLSQKPRFLVGLRAHLLPQGCECRMTCAVQGSPQPHVTWFKNDQSLDRNPAVYSTDLLGVCSLVIPSVSLKDSGEYKAVAKNPLGQAVSTATLIVTEYDS

Target ORF information:

RefSeq Version	XM_017319947.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus immunoglobulin-like and fibronectin type III domain containing 1 (Igfn1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319947.1

ORF Insert Sequence:

ATGGGAACGA GGTGGTGGGC GGCGACCACA AGGGCATCCA ACTGGCTCTG GCAGATGGCT 
ACACATGGCT GTGCCTCCCC AGCGTGTGCA GGAAGGACAG TGGCCGACAA GCCTCAGCCC 
CCACAAGGAC CCCTGGAGGT TCAGGATTGC CACAGAGCAG GTGTCTGCCT CCGCTGGCTA 
CCCCCGAGGG ACAACGGGGG CCAGGTCATA CAACACTATG TGGTGGAGAG GCGACAGGCT 
GGAAGGAGCA CTTGGTTGAA GGTGGGCGAG CCCCCATCAG ACAGTACCAG CTTCACCGAC 
ACCAGCGTGG AGCAGGGAAA GAAATATGCC TTCCGTGTGC GGGCTGTGAC CTCAGTGGGA 
GCCGGAGATG CCCTGGAGTC AGAGGAGGTG TTGGTGGCTC CTGAGGCTCT CCCGGGGCCC 
CCTTCTGCCC CAGCCATCCT GTCAGCCTCC AGTCAGAGCA TCACTCTCAC ATGGGGGGCA 
CCACAGGGCC CTGGCAGTAA CCACATCCTG GGCTACCTGA TCGAGAAGCA CAAGAAGGGG 
AGCAACACCT GGATGGCTGT GAATGAGCAG CCCGTGTCTG AAAGAAGGTA CACTGTGGTG 
GGTCTGCGAC AGGGCTGCCA GTATGAGTTC CGGGTCACGG CCGTGACTCT GTCGGGCCCT 
GGAGATCCTG GGCCTCCATC GGATGCTGTC TTTGCTAGAG ACCCTATGAG ACCCCCAGGG 
CCTGTGCGGG ATCTACAGGT TACAGACACA TCAAACACTA GCATCACCGT GAGCTGGATG 
GTGCCGGATG CCCGGGATGC TGATGAAGCC CAGGGATATA TTGTGGAGTT GTGTGGTTCA 
GACAGCCATC AATGGAGCCC ATGTCACGTG GGAACTGTGC CGGGCACCAC CTTCACAGCC 
AAGGGGCTTC GGCCGCAAGA AGGCTACTTT GTGCGGGTGA CAGCAGTTAA TGATGGGGGC 
CGGAGCCAGG CCACTTCCCT GGACACATTG GTTCATGCCA TGCCGGCCAC TGTCTGTCCC 
AGGTTCCTCA TGGACTCCGG CACAAAGGAT ACCCTGATGG TCAGGGTTGG GGACAGTATT 
CGCGTTCCTC TCTCCTTTGA AGCTGCCCCC ATGCCTGAGG TGACCTGGCT GAAGGACGGC 
TTGCCCTTGC CCAAAAGAAG TGTGACCACT GTGAAGGCTG GCCTCACGCA GCTTCTGATC 
CCTGCAGCTA GCCTCTCGGA CTGTGGCCGA TACACCGTGA TGCTGAGGAA CCTACAGGGG 
AAGGAAGCTA CCCACAGCTT CTTCATCAAT GTAGCAGCAT GCCCACAGGC ACCAGGTTCT 
ATCTACCTAC AGGAGAATGT GCCTGGGACA GTGACGGTCC AGTGGGAACC CTCTCCGGAT 
GAGGCTCAGG GCATCCCCTT GCATTACACA GTGCTGATGC GGTCCTCATC TCACCGATCC 
TGGCACGAGG TGGCCGACCA TGTCCGTACC AACCGCTTTA CCCTCCTAGG GGTCCTCCCT 
GGCCATGAGT ACCACTTTCG GGTGCTGGCC AAGAATGAGC TGGGGGTCAG CAAACCTTCA 
GACACCAGCC AACCCTGGTG TATCCCCAGG CAGAGAGACA GGTTCACAGT GAAGTCTCCG 
ACATACCAGG ATCCTGACCT GAGCCAGAAG CCCCGCTTCC TGGTGGGTCT GCGGGCTCAC 
CTTCTGCCCC AGGGCTGTGA GTGCCGCATG ACGTGTGCCG TGCAGGGCTC ACCCCAGCCT 
CATGTCACCT GGTTCAAGAA TGACCAAAGC TTGGACAGAA ACCCTGCGGT GTACAGTACT 
GACTTGCTGG GTGTGTGCTC CCTCGTCATC CCCAGCGTGT CCCTTAAGGA CAGTGGTGAA 
TACAAGGCTG TGGCCAAGAA CCCACTGGGA CAAGCTGTCA GCACAGCCAC CCTCATTGTC 
ACAGAATACG ACTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Igfn1 ( XM_006529442.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529505.1 Mus musculus immunoglobulin-like and fibronectin type III domain containing 1 (Igfn1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OMu28133
Clone ID Related Accession (Same CDS sequence)	XM_006529442.3
Accession Version	XM_006529442.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	immunoglobulin-like and fibronectin type III domain-containing protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006529442.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCAA AGCCCCCTAA GAAGTCCTCC ATCCCAGGAG TGAGCATCAG GCAGCTGGTG 
GACGAGATCC CAGAAGGCTG CAGCACACCA GATTTCAAGC AGAAGCCTGT TACCTTGGCT 
CTACCAGAGG GGAAAAACGC CATCTTTCGG GCGGTGGTCT GTGGGGAACC CAGACCTGAA 
GTGCACTGGC AGAGCAGCAA AGGAGACCTC AGCAACTCCG CCAAGTACCA GATATCCTCT 
GCTCCCGGTA GAGAGGAGCA CGTGCTTCAG ATCAACAAGC TGACTGGAGA GGATTCGGAT 
CTGTACCGCT GCACTGCACT AAATGCCTAT GGAGAAGCCA CATGCTCAGT GAGGCTTACG 
GTCATTGAAG TGGGTTTTCG GAAGAACCGA AAAAGGCAAA AGGAACCTCA GGAGGACCTC 
AGGAAGGAGC TGATGGACTT TCGGAAGATG CTGAAAAAAC GGGCCCCACC CCCAACCCCT 
GAAAAAAAGA TGGAGTCTGA GCAGGTGTGG CAGCTTTTGG TGACTGCAGA CCGTAAGGAC 
TACGAGAAGA TCTGTATGCA GTATGGTATC GTCGACTTCC GGGGCATGCT GCGCAAGTTG 
CAGGAGATGA AAAAGGAACA GGAGGACAGA ATGGCCCAGT ATGTCAGTGC CATTGCCAAC 
TTGAGACACA TCAAGGTCAC CAAGGAAGGG GTCGCTACGT TTGACCTGGA GCTGGATCTC 
AAGAACCTCG AGAGCAAGAT TTACTTGTAC AAGGATGGTG AGATGATTCC CTACGGTGTT 
GATAACCAGA CCAAGCACTG TCTGAGACGG CTGGGGAAGC GCTACCACTT CCAGATCCAG 
GACCTGCGGC CTGAGGATGC TGGCCTTTAC CAGGTCATGG TGGAGGATGC TGTGGTCTTC 
TCTACAGAGC TAGAATCCAG AACCATCCCC CCCAGAGTGG TGGTCCCATT GGCAGAGACC 
CGCTGTGAGG AACGGGGTGA TGCAGTCTTT GAATGTACCC TCTCCAATCC CTGTCCCAGT 
GCGACCTGGC ATTTCCAGCA CCGACCACTG CGGCTCAGTG ACAAATATGA AGTATTTGTG 
TCCCCTGATG GGCTAACCCA CCGACTGGTT GTGAAGGGGG CCAGTAGCTC AGACATGGGC 
CTCTACTCGC TGAACACTGG GCTCCATGCC TCCAGTGCCT GGCTGGTGGT TGAAGGCTCC 
ATGGACCACT TCTCTCAGGG TCTGACTGAT ACAGAGGTGC AACTGGGGGA AGCCGCCATA 
GTCTCCTGTA CTCTTGCCAG TGACCTGGGA CCTGGCACGT GGTTCAAGGA TGGAGTCAAG 
CTCACTGCCC AAGATGGAGT CATCTTTGAG CAAGATGGGC TCACGCACAG ACTGATTCTC 
ACCCACGTGG AGGGGACCCA GGCTGGGAAA TACACTTTTG TAGCCGGCTG CCAGCACAGC 
GAGGCCAGCC TGACCGTGCA GGATCCCCCC ACCATTGCTC CAGATGTGAC AGAGACACTG 
AGAGAGCCAC TGGTGTTCAA GGCTGGGAAG CCAGTCACCG TGAAAATCCC TTTCCAGAGC 
CGCCTCCCTG TTCAGGCTGC CTGGAGGAAA GATGGGAACG AGGTGGTGGG CGGCGACCAC 
AAGGGCATCC AACTGGCTCT GGCAGATGGC TACACATGGC TGTGCCTCCC CAGCGTGTGC 
AGGAAGGACA GTGGCCGGTA CAGCGTGACG CTGAGGAGCG AGGGAGGCTG TGTGCAGGCT 
GAGTTCACCC TGCAAGTTAT AGACAAGCCT CAGCCCCCAC AAGGACCCCT GGAGGTTCAG 
GATTGCCACA GAGCAGGTGT CTGCCTCCGC TGGCTACCCC CGAGGGACAA CGGGGGCCAG 
GTCATACAAC ACTATGTGGT GGAGAGGCGA CAGGCTGGAA GGAGCACTTG GTTGAAGGTG 
GGCGAGCCCC CATCAGACAG TACCAGCTTC ACCGACACCA GCGTGGAGCA GGGAAAGAAA 
TATGCCTTCC GTGTGCGGGC TGTGACCTCA GTGGGAGCCG GAGATGCCCT GGAGTCAGAG 
GAGGTGTTGG TGGCTCCTGA GGCTCTCCCG GGGCCCCCTT CTGCCCCAGC CATCCTGTCA 
GCCTCCAGTC AGAGCATCAC TCTCACATGG GGGGCACCAC AGGGCCCTGG CAGTAACCAC 
ATCCTGGGCT ACCTGATCGA GAAGCACAAG AAGGGGAGCA ACACCTGGAT GGCTGTGAAT 
GAGCAGCCCG TGTCTGAAAG AAGGTACACT GTGGTGGGTC TGCGACAGGG CTGCCAGTAT 
GAGTTCCGGG TCACGGCCGT GACTCTGTCG GGCCCTGGAG ATCCTGGGCC TCCATCGGAT 
GCTGTCTTTG CTAGAGACCC TATGAGACCC CCAGGGCCTG TGCGGGATCT ACAGGTTACA 
GACACATCAA ACACTAGCAT CACCGTGAGC TGGATGGTGC CGGATGCCCG GGATGCTGAT 
GAAGCCCAGG GATATATTGT GGAGTTGTGT GGTTCAGACA GCCATCAATG GAGCCCATGT 
CACGTGGGAA CTGTGCCGGG CACCACCTTC ACAGCCAAGG GGCTTCGGCC GCAAGAAGGC 
TACTTTGTGC GGGTGACAGC AGTTAATGAT GGGGGCCGGA GCCAGGCCAC TTCCCTGGAC 
ACATTGGTTC ATGCCATGCC GGCCACTGTC TGTCCCAGGT TCCTCATGGA CTCCGGCACA 
AAGGATACCC TGATGGTCAG GGTTGGGGAC AGTATTCGCG TTCCTCTCTC CTTTGAAGCT 
GCCCCCATGC CTGAGGTGAC CTGGCTGAAG GACGGCTTGC CCTTGCCCAA AAGAAGTGTG 
ACCACTGTGA AGGCTGGCCT CACGCAGCTT CTGATCCCTG CAGCTAGCCT CTCGGACTGT 
GGCCGATACA CCGTGATGCT GAGGAACCTA CAGGGGAAGG AAGCTACCCA CAGCTTCTTC 
ATCAATGTAG CAGCATGCCC ACAGGCACCA GGTTCTATCT ACCTACAGGA GAATGTGCCT 
GGGACAGTGA CGGTCCAGTG GGAACCCTCT CCGGATGAGG CTCAGGGCAT CCCCTTGCAT 
TACACAGTGC TGATGCGGTC CTCATCTCAC CGATCCTGGC ACGAGGTGGC CGACCATGTC 
CGTACCAACC GCTTTACCCT CCTAGGGGTC CTCCCTGGCC ATGAGTACCA CTTTCGGGTG 
CTGGCCAAGA ATGAGCTGGG GGTCAGCAAA CCTTCAGACA CCAGCCAACC CTGGTGTATC 
CCCAGGCAGA GAGACAGGTT CACAGTGAAG TCTCCGACAT ACCAGGATCC TGACCTGAGC 
CAGAAGCCCC GCTTCCTGGT GGGTCTGCGG GCTCACCTTC TGCCCCAGGG CTGTGAGTGC 
CGCATGACGT GTGCCGTGCA GGGCTCACCC CAGCCTCATG TCACCTGGTT CAAGAATGAC 
CAAAGCTTGG ACAGAAACCC TGCGGTGTAC AGTACTGACT TGCTGGGTGT GTGCTCCCTC 
GTCATCCCCA GCGTGTCCCT TAAGGACAGT GGTGAATACA AGGCTGTGGC CAAGAACCCA 
CTGGGACAAG CTGTCAGCAC AGCCACCCTC ATTGTCACAG AATACGACTC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529505.1
CDS	567..4160
Translation	MAAKPPKKSSIPGVSIRQLVDEIPEGCSTPDFKQKPVTLALPEGKNAIFRAVVCGEPRPEVHWQSSKGDLSNSAKYQISSAPGREEHVLQINKLTGEDSDLYRCTALNAYGEATCSVRLTVIEVGFRKNRKRQKEPQEDLRKELMDFRKMLKKRAPPPTPEKKMESEQVWQLLVTADRKDYEKICMQYGIVDFRGMLRKLQEMKKEQEDRMAQYVSAIANLRHIKVTKEGVATFDLELDLKNLESKIYLYKDGEMIPYGVDNQTKHCLRRLGKRYHFQIQDLRPEDAGLYQVMVEDAVVFSTELESRTIPPRVVVPLAETRCEERGDAVFECTLSNPCPSATWHFQHRPLRLSDKYEVFVSPDGLTHRLVVKGASSSDMGLYSLNTGLHASSAWLVVEGSMDHFSQGLTDTEVQLGEAAIVSCTLASDLGPGTWFKDGVKLTAQDGVIFEQDGLTHRLILTHVEGTQAGKYTFVAGCQHSEASLTVQDPPTIAPDVTETLREPLVFKAGKPVTVKIPFQSRLPVQAAWRKDGNEVVGGDHKGIQLALADGYTWLCLPSVCRKDSGRYSVTLRSEGGCVQAEFTLQVIDKPQPPQGPLEVQDCHRAGVCLRWLPPRDNGGQVIQHYVVERRQAGRSTWLKVGEPPSDSTSFTDTSVEQGKKYAFRVRAVTSVGAGDALESEEVLVAPEALPGPPSAPAILSASSQSITLTWGAPQGPGSNHILGYLIEKHKKGSNTWMAVNEQPVSERRYTVVGLRQGCQYEFRVTAVTLSGPGDPGPPSDAVFARDPMRPPGPVRDLQVTDTSNTSITVSWMVPDARDADEAQGYIVELCGSDSHQWSPCHVGTVPGTTFTAKGLRPQEGYFVRVTAVNDGGRSQATSLDTLVHAMPATVCPRFLMDSGTKDTLMVRVGDSIRVPLSFEAAPMPEVTWLKDGLPLPKRSVTTVKAGLTQLLIPAASLSDCGRYTVMLRNLQGKEATHSFFINVAACPQAPGSIYLQENVPGTVTVQWEPSPDEAQGIPLHYTVLMRSSSHRSWHEVADHVRTNRFTLLGVLPGHEYHFRVLAKNELGVSKPSDTSQPWCIPRQRDRFTVKSPTYQDPDLSQKPRFLVGLRAHLLPQGCECRMTCAVQGSPQPHVTWFKNDQSLDRNPAVYSTDLLGVCSLVIPSVSLKDSGEYKAVAKNPLGQAVSTATLIVTEYDS

Target ORF information:

RefSeq Version	XM_006529442.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus immunoglobulin-like and fibronectin type III domain containing 1 (Igfn1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529442.3

ORF Insert Sequence:

ATGGCTGCAA AGCCCCCTAA GAAGTCCTCC ATCCCAGGAG TGAGCATCAG GCAGCTGGTG 
GACGAGATCC CAGAAGGCTG CAGCACACCA GATTTCAAGC AGAAGCCTGT TACCTTGGCT 
CTACCAGAGG GGAAAAACGC CATCTTTCGG GCGGTGGTCT GTGGGGAACC CAGACCTGAA 
GTGCACTGGC AGAGCAGCAA AGGAGACCTC AGCAACTCCG CCAAGTACCA GATATCCTCT 
GCTCCCGGTA GAGAGGAGCA CGTGCTTCAG ATCAACAAGC TGACTGGAGA GGATTCGGAT 
CTGTACCGCT GCACTGCACT AAATGCCTAT GGAGAAGCCA CATGCTCAGT GAGGCTTACG 
GTCATTGAAG TGGGTTTTCG GAAGAACCGA AAAAGGCAAA AGGAACCTCA GGAGGACCTC 
AGGAAGGAGC TGATGGACTT TCGGAAGATG CTGAAAAAAC GGGCCCCACC CCCAACCCCT 
GAAAAAAAGA TGGAGTCTGA GCAGGTGTGG CAGCTTTTGG TGACTGCAGA CCGTAAGGAC 
TACGAGAAGA TCTGTATGCA GTATGGTATC GTCGACTTCC GGGGCATGCT GCGCAAGTTG 
CAGGAGATGA AAAAGGAACA GGAGGACAGA ATGGCCCAGT ATGTCAGTGC CATTGCCAAC 
TTGAGACACA TCAAGGTCAC CAAGGAAGGG GTCGCTACGT TTGACCTGGA GCTGGATCTC 
AAGAACCTCG AGAGCAAGAT TTACTTGTAC AAGGATGGTG AGATGATTCC CTACGGTGTT 
GATAACCAGA CCAAGCACTG TCTGAGACGG CTGGGGAAGC GCTACCACTT CCAGATCCAG 
GACCTGCGGC CTGAGGATGC TGGCCTTTAC CAGGTCATGG TGGAGGATGC TGTGGTCTTC 
TCTACAGAGC TAGAATCCAG AACCATCCCC CCCAGAGTGG TGGTCCCATT GGCAGAGACC 
CGCTGTGAGG AACGGGGTGA TGCAGTCTTT GAATGTACCC TCTCCAATCC CTGTCCCAGT 
GCGACCTGGC ATTTCCAGCA CCGACCACTG CGGCTCAGTG ACAAATATGA AGTATTTGTG 
TCCCCTGATG GGCTAACCCA CCGACTGGTT GTGAAGGGGG CCAGTAGCTC AGACATGGGC 
CTCTACTCGC TGAACACTGG GCTCCATGCC TCCAGTGCCT GGCTGGTGGT TGAAGGCTCC 
ATGGACCACT TCTCTCAGGG TCTGACTGAT ACAGAGGTGC AACTGGGGGA AGCCGCCATA 
GTCTCCTGTA CTCTTGCCAG TGACCTGGGA CCTGGCACGT GGTTCAAGGA TGGAGTCAAG 
CTCACTGCCC AAGATGGAGT CATCTTTGAG CAAGATGGGC TCACGCACAG ACTGATTCTC 
ACCCACGTGG AGGGGACCCA GGCTGGGAAA TACACTTTTG TAGCCGGCTG CCAGCACAGC 
GAGGCCAGCC TGACCGTGCA GGATCCCCCC ACCATTGCTC CAGATGTGAC AGAGACACTG 
AGAGAGCCAC TGGTGTTCAA GGCTGGGAAG CCAGTCACCG TGAAAATCCC TTTCCAGAGC 
CGCCTCCCTG TTCAGGCTGC CTGGAGGAAA GATGGGAACG AGGTGGTGGG CGGCGACCAC 
AAGGGCATCC AACTGGCTCT GGCAGATGGC TACACATGGC TGTGCCTCCC CAGCGTGTGC 
AGGAAGGACA GTGGCCGGTA CAGCGTGACG CTGAGGAGCG AGGGAGGCTG TGTGCAGGCT 
GAGTTCACCC TGCAAGTTAT AGACAAGCCT CAGCCCCCAC AAGGACCCCT GGAGGTTCAG 
GATTGCCACA GAGCAGGTGT CTGCCTCCGC TGGCTACCCC CGAGGGACAA CGGGGGCCAG 
GTCATACAAC ACTATGTGGT GGAGAGGCGA CAGGCTGGAA GGAGCACTTG GTTGAAGGTG 
GGCGAGCCCC CATCAGACAG TACCAGCTTC ACCGACACCA GCGTGGAGCA GGGAAAGAAA 
TATGCCTTCC GTGTGCGGGC TGTGACCTCA GTGGGAGCCG GAGATGCCCT GGAGTCAGAG 
GAGGTGTTGG TGGCTCCTGA GGCTCTCCCG GGGCCCCCTT CTGCCCCAGC CATCCTGTCA 
GCCTCCAGTC AGAGCATCAC TCTCACATGG GGGGCACCAC AGGGCCCTGG CAGTAACCAC 
ATCCTGGGCT ACCTGATCGA GAAGCACAAG AAGGGGAGCA ACACCTGGAT GGCTGTGAAT 
GAGCAGCCCG TGTCTGAAAG AAGGTACACT GTGGTGGGTC TGCGACAGGG CTGCCAGTAT 
GAGTTCCGGG TCACGGCCGT GACTCTGTCG GGCCCTGGAG ATCCTGGGCC TCCATCGGAT 
GCTGTCTTTG CTAGAGACCC TATGAGACCC CCAGGGCCTG TGCGGGATCT ACAGGTTACA 
GACACATCAA ACACTAGCAT CACCGTGAGC TGGATGGTGC CGGATGCCCG GGATGCTGAT 
GAAGCCCAGG GATATATTGT GGAGTTGTGT GGTTCAGACA GCCATCAATG GAGCCCATGT 
CACGTGGGAA CTGTGCCGGG CACCACCTTC ACAGCCAAGG GGCTTCGGCC GCAAGAAGGC 
TACTTTGTGC GGGTGACAGC AGTTAATGAT GGGGGCCGGA GCCAGGCCAC TTCCCTGGAC 
ACATTGGTTC ATGCCATGCC GGCCACTGTC TGTCCCAGGT TCCTCATGGA CTCCGGCACA 
AAGGATACCC TGATGGTCAG GGTTGGGGAC AGTATTCGCG TTCCTCTCTC CTTTGAAGCT 
GCCCCCATGC CTGAGGTGAC CTGGCTGAAG GACGGCTTGC CCTTGCCCAA AAGAAGTGTG 
ACCACTGTGA AGGCTGGCCT CACGCAGCTT CTGATCCCTG CAGCTAGCCT CTCGGACTGT 
GGCCGATACA CCGTGATGCT GAGGAACCTA CAGGGGAAGG AAGCTACCCA CAGCTTCTTC 
ATCAATGTAG CAGCATGCCC ACAGGCACCA GGTTCTATCT ACCTACAGGA GAATGTGCCT 
GGGACAGTGA CGGTCCAGTG GGAACCCTCT CCGGATGAGG CTCAGGGCAT CCCCTTGCAT 
TACACAGTGC TGATGCGGTC CTCATCTCAC CGATCCTGGC ACGAGGTGGC CGACCATGTC 
CGTACCAACC GCTTTACCCT CCTAGGGGTC CTCCCTGGCC ATGAGTACCA CTTTCGGGTG 
CTGGCCAAGA ATGAGCTGGG GGTCAGCAAA CCTTCAGACA CCAGCCAACC CTGGTGTATC 
CCCAGGCAGA GAGACAGGTT CACAGTGAAG TCTCCGACAT ACCAGGATCC TGACCTGAGC 
CAGAAGCCCC GCTTCCTGGT GGGTCTGCGG GCTCACCTTC TGCCCCAGGG CTGTGAGTGC 
CGCATGACGT GTGCCGTGCA GGGCTCACCC CAGCCTCATG TCACCTGGTT CAAGAATGAC 
CAAAGCTTGG ACAGAAACCC TGCGGTGTAC AGTACTGACT TGCTGGGTGT GTGCTCCCTC 
GTCATCCCCA GCGTGTCCCT TAAGGACAGT GGTGAATACA AGGCTGTGGC CAAGAACCCA 
CTGGGACAAG CTGTCAGCAC AGCCACCCTC ATTGTCACAG AATACGACTC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Igfn1 ( XM_017319943.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175432.1 Mus musculus immunoglobulin-like and fibronectin type III domain containing 1 (Igfn1), transcript variant X4, mRNA.

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CloneID	OMu77089
Clone ID Related Accession (Same CDS sequence)	XM_017319943.1
Accession Version	XM_017319943.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	immunoglobulin-like and fibronectin type III domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGGA AAAACGCCAT CTTTCGGGCG GTGGTCTGTG GGGAACCCAG ACCTGAAGTG 
CACTGGCAGA GCAGCAAAGG AGACCTCAGC AACTCCGCCA AGTACCAGAT ATCCTCTGCT 
CCCGGTAGAG AGGAGCACGT GCTTCAGATC AACAAGCTGA CTGGAGAGGA TTCGGATCTG 
TACCGCTGCA CTGCACTAAA TGCCTATGGA GAAGCCACAT GCTCAGTGAG GCTTACGGTC 
ATTGAAGTGG GTTTTCGGAA GAACCGAAAA AGGCAAAAGG AACCTCAGGA GGACCTCAGG 
AAGGAGCTGA TGGACTTTCG GAAGATGCTG AAAAAACGGG CCCCACCCCC AACCCCTGAA 
AAAAAGATGG AGTCTGAGCA GGTGTGGCAG CTTTTGGTGA CTGCAGACCG TAAGGACTAC 
GAGAAGATCT GTATGCAGTA TGGTATCGTC GACTTCCGGG GCATGCTGCG CAAGTTGCAG 
GAGATGAAAA AGGAACAGGA GGACAGAATG GCCCAGTATG TCAGTGCCAT TGCCAACTTG 
AGACACATCA AGGTCACCAA GGAAGGGGTC GCTACGTTTG ACCTGGAGCT GGATCTCAAG 
AACCTCGAGA GCAAGATTTA CTTGTACAAG GATGGTGAGA TGATTCCCTA CGGTGTTGAT 
AACCAGACCA AGCACTGTCT GAGACGGCTG GGGAAGCGCT ACCACTTCCA GATCCAGGAC 
CTGCGGCCTG AGGATGCTGG CCTTTACCAG GTCATGGTGG AGGATGCTGT GGTCTTCTCT 
ACAGAGCTAG AATCCAGAAC CATCCCCCCC AGAGTGGTGG TCCCATTGGC AGAGACCCGC 
TGTGAGGAAC GGGGTGATGC AGTCTTTGAA TGTACCCTCT CCAATCCCTG TCCCAGTGCG 
ACCTGGCATT TCCAGCACCG ACCACTGCGG CTCAGTGACA AATATGAAGT ATTTGTGTCC 
CCTGATGGGC TAACCCACCG ACTGGTTGTG AAGGGGGCCA GTAGCTCAGA CATGGGCCTC 
TACTCGCTGA ACACTGGGCT CCATGCCTCC AGTGCCTGGC TGGTGGTTGA AGGTGGGAAG 
GATAAAGGCC CTCAGACCAC AGATACTGAC CACCGGCTGC AGACTCCAGA AGCTCTGGCC 
TCCGAAGCAG AAGACGCTGG AGGCATCAGC ATCAAGGGAG GGCAATCCAG AGAGCGGGGT 
TCCCTCAAAG AGATTAGTGG GGCACAGCTC ACAGCAGGCC CAGACAGGGG TGCCTTTGGC 
AAACATGGCT ATCCCTTGGT GGTGGATGAA GACATAGTTA ATTCTACTTG GGGACCTGGG 
CAGGACAAAA AGAGCTTTCT GGAAGGAGGA CAACTCAGGG TCACTCTTCC AGGGGATAGT 
CAGTCCCAAA GAGAGGGAGA CTGTGGTAGA AGCCTCCCCA GAAGGTCTCA TCTCCAGGGA 
GAGGATACAG AATCAGATTT GGGCTTCCTG GAGAGAGGAC AACAAGGGCC TGGTAGAGTT 
GACAGTGAGA ATGGCAGATG GGAGACAGCA GGAGGCCAGA GTGCTGGCTC CAGCCATCCT 
CGGGACAGAA GGCTTGAGAG CAGAGGAGAA GGACAGGAAC ACAGTGAGGG CCATGGCAGT 
GAACTGGACA GATATGGCCA GGGACAATGC CATGATCCTC AGCTGGGAGC AGGGGCAGGG 
CAGAGGGTGC CGTGGGGATC CCAGTTTGAT GCTGAGGGGC TTCAGCTGAA AAAGGAGGGG 
ACAGAAATGT GGGGCGATTG TCTGGATGTG ACGGAAGGTA GGGGTAACCT GAGAAAGGAG 
AGGGGAACAG CAGCAGGGTC AAGTAACAGA GCCCGCCTCT GGGAAGCTCG AGGAGGCAGT 
GGGGCCGGAA GTCCTTGCGT CCCTGAGTTT CCTAGAGGAG GAGCTCACAG CTCCAAGGCA 
GGCATGGGCC CTGAGTGCTG GCCAGGTGGA GATGCTGGCC ATGGAGAAGC TGGGGTATAC 
TGGGGGGCAG GGGGATATCC AGGACAGACA TCTGAGGGCA ATGACACCCA GAAATCATCA 
CTATCTGGAG ACAGGAAACT TCTGGGGTGC ACGAGTCCTG AGGCCAAAGC TGAGGGCTCT 
TTCCAGAGCG CAGATGGTCA TGGGATTGTG GGGAGGGTCT ACAAACCTGG CCCTGAAGGC 
CCAGAAGATC TTAGGAGTCA GAAAAGTGGC CTACAAGAGA TTCAGGGAAG GAATGGTCAA 
GGCTCAGCTG GAGCCTTGGG CAGGACGGTG GAAGGTTCCA GAAGCCTCCA ATTTCCTCAG 
AGCTGGACAG TGGGACAGAG GGAAGCAGGG AAGCCAGGTG GAGCAGAGTA TGAAGACATA 
GGTCCTCAGG ATGACACGTG GTCCAATCTC AGAGAGATGG GAGGCCACTG TGGGTCTGGA 
GTGCTGGCGT CTGGTGGGAG GGAAGGGAGC GTGGGCGGTT CCCAAGTAGC TGCCCTGATG 
ATGTCTAGTC AGAGGGTAGA TGCCAGAAAC CACAGGCTAG TTACATCCCC AGGCCTGGGT 
GTCCAGGGTT CTGGCAGAAC ACTGGGACAC ATGGAAGGAT TAAGAGACCC AGAGGCAGTG 
GGATCCGAAG AAGAGTTCTG GAATGGGTCA GGAAGGTCCC AAGGAAAAGG GTCCAGAGGT 
GGGATGGGCT TAGGAGGTCC AGAGTGGGCA GAGTCTAGGA ATGAGGAGGG ATGGGAGAAT 
TCTGAAGGGA TGGCTTCTAG GAATGGAGCC ACTTGTCGTG ATGGATCCAG GATGCAGGAA 
GGAACAGGAT CTGGCCCTGA TATTGGTTGC AAGGCCCCCT GTGGGGCACC TGCGGAGACA 
GAGTCTGGGA AGTGGGGTAC TTACAGTCAT GACTCAGGGG TGCCTGGGGG ATTGTGGTCT 
GGAAGCAAAA GTCAGTGTAG TCCTGCAGGA AAGGTATCTG GAAGAGAGGC CAGCCTCAGA 
AATGGCTCAG CTGGCCTTCA TGGAATGCCT TCTAGTGAAG CTCAGCAGAG GGATGTATTT 
CAAGGCCACG GTCAGACAGG CCACATTGGA GAGCACCACT GTGCCCGTGG CTTAGGGAGT 
TCTGAGACAG TGGGATCTGT GGGTGGACAT GGTAGGAAAG ACTCTGGAAC AGCGGGCAAG 
GTTGGAGAGG GATACACAGA AGCAGAACCA GGGCACTCTG GAGGGTTGAG TTCTTGGGGT 
CACACTGGAG ATTATGAGGA CTTCCGAGTC CTGGGGGCCT TTAGGGAAGG AGACTTTGGC 
AATGGCACTG GGGTTCCATT ATCCATGGGA CCAAGGTCTC TGGAGAGAGG GGACAAGGAA 
GGAGATGGAG AAAGGATCAG TTTTCTTGGT GCCAGGACCT CTACAGTTGG AACTGGGAAT 
TGGGACAAAG CCAGGCACCC TGGGGCACCA TCTCCTCATG AGGCCGGTTC AGAGGGCCAC 
TGGGCTCAAG TGTCTGGCAA TGCATTAGGT TGTAGAGATG GGTCTGGAAT TCCAGAGCCT 
TGGAGTGCTG GCGACAAAAC AGCTTATGGA GAAGAATCAA AGGGTCTTGG GCCTGAAAGA 
ACAGGACCAG ATGGTGAGGT GGCATTTAGA GGTAGCTCTT CGAGCCTCAG AGGAATGGGA 
CCAGCAAGTG AGGCAAGTTA TAAAAGTGGA ACTTGGGGCC CTGGGGCTGT GGGTTCAGGG 
TTTAAGGTAG ATTGTAGGAA TGATCTTGGG TGTTCTGGAA GTATGGGGTC AGAGAGTAAA 
GCAGGTTATA AGAACAAAAT TGAGAGTCAT GGGGTCAGGG AGTTAGGGGA CCTGACAGGC 
TATAAAAATG ATTTAGGACC TTCTGAACTT ATTTCATCAA GGAATGAGGC ACGTGGTTTA 
GAACATTCTG GAGACATTTT GTCATGGAAT AAGGCAGGTT TAAGGGATGG CTTGGGAGAA 
ACAGGAAGAA TGGAGTCCAA GAATGGGGTT GGTTATAGGG GAAGCTCAGT GGGTCCTGGG 
GAAATGGGGT CAGAGAGGAA AATGCACCTT GCAGATGGTT CAGGGAGGCT AGGAGGACCA 
GGGTCACTGG CTGCGCCCAA AGAACATGAA TCTGTGGGTC TTGGGTCAGT TTATGAGGGA 
GCAGCAGAGA TTGGCTCCAA ATATAATAGG GAGAAAGTCA GAGGAATGGG GTTTAGAGAT 
GCAACAGGGC CTGAGGTGGG CTCTGGGGTA GCTGGAATGC TGGATATTAC AGGTGGCATA 
GCACACGGGG ACAGTGCTAT GGGCACAGAG AAGCTGGGGA CACCAGATAG GCCTTATATC 
TTGTCAGGTG GGCAGGGTAC CAAGAACAGT CTTGGGGGCT GTGTGTCCTT GGGAATCCCT 
GACGCCTTGG GGGCTGTTGG CTCTGTGGGG AAACCAGGTA TCAGAGAATG GAAAGATGAT 
TCTGGGTTCC AAGGGTCTCT CAGGGACAGA GGGACTCCGA GTGAGGAGAT CAGATCTGTA 
GACCAAGCAG GAGCTATAGG GACATCTCGG CTTCTTGATA GTAGGGGGGC AGTGGAGGAT 
GGGAGTGGGT CAGAAAATGA CTTTGACTTT AATAAATCAG GCTTGGAGAC AACAGGAAGA 
TGTAGAGTTG CTGACCAGCA GGGAGTGACA CCACAGGGAT GTGGAGGCTC ATTGCTCAAT 
GGCAGGAGGA AGGGAACAAG TTCTGGAAGC TTGTCAGGGG TAGGGCAGGA GGTAGACAGA 
AGTTCTACAC CTGGGAGGGA GACCAAGGTA ATGTCAGGAC CTGGCAGTGG GCGAGCCACA 
AGCCACACTC GGGCTGCAGA CTGGGAAGAC GGGGTTCAAT GCAGCTTCGG GGCTTCTAGC 
TCACTGCCTG ATACTGATGC CATTTTCAGA GAAACCCATG CGGGACAAGG AGCCTTCAAG 
GGCAGGGGCT GTGAAACCGG CCAAGGGGCT GCTGGAGAGT GTCAAGGACC ACGCTCCTTG 
GGGAGCAGAG GCTCAGAGTT TGTAGGGGGC AGGGCTGGAT TTACAGGGAG CTCAAGTGTT 
CCAGGCAGAA GGGATTCCAC TATCTACGGA GATGCTGTCA CATCAAAGCC ACAGGAACCC 
CAGAATGAAT TATATAGTCC TTTAGGCAGA AGGGACTTCA AAAGTGTATC GGGAGGAATC 
CAGGAGCCAG GTTTTCAACG AGGAACAGGA CAGGGAGAAG GAAAAGAATC CTTCCAGGAG 
TCAGGGTCCT GGGAGGCTGA GCTTGACGAG GTCCAAGGAT CCAGGGCCTC AGCAAAGTTT 
GAAAACCAGG GAGGGAAAGA CCCTGGCTGG TCAGGCCAGA AGCCTGGTCC CTGTGGAAGC 
AGGCATCAGG TGCAGTCTGG GACTGAAGTT GGATCAGCAA AGAGAGCAAC CCCACAGGGG 
GCCGGAGGGC TGGAGCCTTG GGCTGGCAGT GAGGATAGAG GGTCTCTGAG AGAACCTTGG 
AGTGAAGACA GGAGGCAAGG CCCCCACAGG CACCTTGGCA GCAGGAGAGA TACCCAAGAG 
GGCAGGTCAG ATGTCTGTGG CCAGGCACAA GATGCTACCC AGAGCCCCAG ATCCAGATAC 
CAGCCTGGCA CTGGCCGCTC TTCTTCAGAG GCCCGAGGCT CCATGGACCA CTTCTCTCAG 
GGTCTGACTG ATACAGAGGT GCAACTGGGG GAAGCCGCCA TAGTCTCCTG TACTCTTGCC 
AGTGACCTGG GACCTGGCAC GTGGTTCAAG GATGGAGTCA AGCTCACTGC CCAAGATGGA 
GTCATCTTTG AGCAAGATGG GCTCACGCAC AGACTGATTC TCACCCACGT GGAGGGGACC 
CAGGCTGGGA AATACACTTT TGTAGCCGGC TGCCAGCACA GCGAGGCCAG CCTGACCGTG 
CAGGATCCCC CCACCATTGC TCCAGATGTG ACAGAGACAC TGAGAGAGCC ACTGGTGTTC 
AAGGCTGGGA AGCCAGTCAC CGTGAAAATC CCTTTCCAGA GCCGCCTCCC TGTTCAGGCT 
GCCTGGAGGA AAGATGGGAA CGAGGTGGTG GGCGGCGACC ACAAGGGCAT CCAACTGGCT 
CTGGCAGATG GCTACACATG GCTGTGCCTC CCCAGCGTGT GCAGGAAGGA CAGTGGCCGG 
TACAGCGTGA CGCTGAGGAG CGAGGGAGGC TGTGTGCAGG CTGAGTTCAC CCTGCAAGTT 
ATAGACAAGC CTCAGCCCCC ACAAGGACCC CTGGAGGTTC AGGATTGCCA CAGAGCAGGT 
GTCTGCCTCC GCTGGCTACC CCCGAGGGAC AACGGGGGCC AGGTCATACA ACACTATGTG 
GTGGAGAGGC GACAGGCTGG AAGGAGCACT TGGTTGAAGG TGGGCGAGCC CCCATCAGAC 
AGTACCAGCT TCACCGACAC CAGCGTGGAG CAGGGAAAGA AATATGCCTT CCGTGTGCGG 
GCTGTGACCT CAGTGGGAGC CGGAGATGCC CTGGAGTCAG AGGAGGTGTT GGTGGCTCCT 
GAGGCTCTCC CGGGGCCCCC TTCTGCCCCA GCCATCCTGT CAGCCTCCAG TCAGAGCATC 
ACTCTCACAT GGGGGGCACC ACAGGGCCCT GGCAGTAACC ACATCCTGGG CTACCTGATC 
GAGAAGCACA AGAAGGGGAG CAACACCTGG ATGGCTGTGA ATGAGCAGCC CGTGTCTGAA 
AGAAGGTACA CTGTGGTGGG TCTGCGACAG GGCTGCCAGT ATGAGTTCCG GGTCACGGCC 
GTGACTCTGT CGGGCCCTGG AGATCCTGGG CCTCCATCGG ATGCTGTCTT TGCTAGAGAC 
CCTATGAGAC CCCCAGGGCC TGTGCGGGAT CTACAGGTTA CAGACACATC AAACACTAGC 
ATCACCGTGA GCTGGATGGT GCCGGATGCC CGGGATGCTG ATGAAGCCCA GGGATATATT 
GTGGAGTTGT GTGGTTCAGA CAGCCATCAA TGGAGCCCAT GTCACGTGGG AACTGTGCCG 
GGCACCACCT TCACAGCCAA GGGGCTTCGG CCGCAAGAAG GCTACTTTGT GCGGGTGACA 
GCAGTTAATG ATGGGGGCCG GAGCCAGGCC ACTTCCCTGG ACACATTGGT TCATGCCATG 
CCGGCCACTG TCTGTCCCAG GTTCCTCATG GACTCCGGCA CAAAGGATAC CCTGATGGTC 
AGGGTTGGGG ACAGTATTCG CGTTCCTCTC TCCTTTGAAG CTGCCCCCAT GCCTGAGGTG 
ACCTGGCTGA AGGACGGCTT GCCCTTGCCC AAAAGAAGTG TGACCACTGT GAAGGCTGGC 
CTCACGCAGC TTCTGATCCC TGCAGCTAGC CTCTCGGACT GTGGCCGATA CACCGTGATG 
CTGAGGAACC TACAGGGGAA GGAAGCTACC CACAGCTTCT TCATCAATGT AGCAGCATGC 
CCACAGGCAC CAGGTTCTAT CTACCTACAG GAGAATGTGC CTGGGACAGT GACGGTCCAG 
TGGGAACCCT CTCCGGATGA GGCTCAGGGC ATCCCCTTGC ATTACACAGT GCTGATGCGG 
TCCTCATCTC ACCGATCCTG GCACGAGGTG GCCGACCATG TCCGTACCAA CCGCTTTACC 
CTCCTAGGGG TCCTCCCTGG CCATGAGTAC CACTTTCGGG TGCTGGCCAA GAATGAGCTG 
GGGGTCAGCA AACCTTCAGA CACCAGCCAA CCCTGGTGTA TCCCCAGGCA GAGAGACAGG 
TTCACAGTGA AGTCTCCGAC ATACCAGGAT CCTGACCTGA GCCAGAAGCC CCGCTTCCTG 
GTGGGTCTGC GGGCTCACCT TCTGCCCCAG GGCTGTGAGT GCCGCATGAC GTGTGCCGTG 
CAGGGCTCAC CCCAGCCTCA TGTCACCTGG TTCAAGAATG ACCAAAGCTT GGACAGAAAC 
CCTGCGGTGT ACAGTACTGA CTTGCTGGGT GTGTGCTCCC TCGTCATCCC CAGCGTGTCC 
CTTAAGGACA GTGGTGAATA CAAGGCTGTG GCCAAGAACC CACTGGGACA AGCTGTCAGC 
ACAGCCACCC TCATTGTCAC AGAATACGAC TCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175432.1
CDS	567..8522
Translation	MAGKNAIFRAVVCGEPRPEVHWQSSKGDLSNSAKYQISSAPGREEHVLQINKLTGEDSDLYRCTALNAYGEATCSVRLTVIEVGFRKNRKRQKEPQEDLRKELMDFRKMLKKRAPPPTPEKKMESEQVWQLLVTADRKDYEKICMQYGIVDFRGMLRKLQEMKKEQEDRMAQYVSAIANLRHIKVTKEGVATFDLELDLKNLESKIYLYKDGEMIPYGVDNQTKHCLRRLGKRYHFQIQDLRPEDAGLYQVMVEDAVVFSTELESRTIPPRVVVPLAETRCEERGDAVFECTLSNPCPSATWHFQHRPLRLSDKYEVFVSPDGLTHRLVVKGASSSDMGLYSLNTGLHASSAWLVVEGGKDKGPQTTDTDHRLQTPEALASEAEDAGGISIKGGQSRERGSLKEISGAQLTAGPDRGAFGKHGYPLVVDEDIVNSTWGPGQDKKSFLEGGQLRVTLPGDSQSQREGDCGRSLPRRSHLQGEDTESDLGFLERGQQGPGRVDSENGRWETAGGQSAGSSHPRDRRLESRGEGQEHSEGHGSELDRYGQGQCHDPQLGAGAGQRVPWGSQFDAEGLQLKKEGTEMWGDCLDVTEGRGNLRKERGTAAGSSNRARLWEARGGSGAGSPCVPEFPRGGAHSSKAGMGPECWPGGDAGHGEAGVYWGAGGYPGQTSEGNDTQKSSLSGDRKLLGCTSPEAKAEGSFQSADGHGIVGRVYKPGPEGPEDLRSQKSGLQEIQGRNGQGSAGALGRTVEGSRSLQFPQSWTVGQREAGKPGGAEYEDIGPQDDTWSNLREMGGHCGSGVLASGGREGSVGGSQVAALMMSSQRVDARNHRLVTSPGLGVQGSGRTLGHMEGLRDPEAVGSEEEFWNGSGRSQGKGSRGGMGLGGPEWAESRNEEGWENSEGMASRNGATCRDGSRMQEGTGSGPDIGCKAPCGAPAETESGKWGTYSHDSGVPGGLWSGSKSQCSPAGKVSGREASLRNGSAGLHGMPSSEAQQRDVFQGHGQTGHIGEHHCARGLGSSETVGSVGGHGRKDSGTAGKVGEGYTEAEPGHSGGLSSWGHTGDYEDFRVLGAFREGDFGNGTGVPLSMGPRSLERGDKEGDGERISFLGARTSTVGTGNWDKARHPGAPSPHEAGSEGHWAQVSGNALGCRDGSGIPEPWSAGDKTAYGEESKGLGPERTGPDGEVAFRGSSSSLRGMGPASEASYKSGTWGPGAVGSGFKVDCRNDLGCSGSMGSESKAGYKNKIESHGVRELGDLTGYKNDLGPSELISSRNEARGLEHSGDILSWNKAGLRDGLGETGRMESKNGVGYRGSSVGPGEMGSERKMHLADGSGRLGGPGSLAAPKEHESVGLGSVYEGAAEIGSKYNREKVRGMGFRDATGPEVGSGVAGMLDITGGIAHGDSAMGTEKLGTPDRPYILSGGQGTKNSLGGCVSLGIPDALGAVGSVGKPGIREWKDDSGFQGSLRDRGTPSEEIRSVDQAGAIGTSRLLDSRGAVEDGSGSENDFDFNKSGLETTGRCRVADQQGVTPQGCGGSLLNGRRKGTSSGSLSGVGQEVDRSSTPGRETKVMSGPGSGRATSHTRAADWEDGVQCSFGASSSLPDTDAIFRETHAGQGAFKGRGCETGQGAAGECQGPRSLGSRGSEFVGGRAGFTGSSSVPGRRDSTIYGDAVTSKPQEPQNELYSPLGRRDFKSVSGGIQEPGFQRGTGQGEGKESFQESGSWEAELDEVQGSRASAKFENQGGKDPGWSGQKPGPCGSRHQVQSGTEVGSAKRATPQGAGGLEPWAGSEDRGSLREPWSEDRRQGPHRHLGSRRDTQEGRSDVCGQAQDATQSPRSRYQPGTGRSSSEARGSMDHFSQGLTDTEVQLGEAAIVSCTLASDLGPGTWFKDGVKLTAQDGVIFEQDGLTHRLILTHVEGTQAGKYTFVAGCQHSEASLTVQDPPTIAPDVTETLREPLVFKAGKPVTVKIPFQSRLPVQAAWRKDGNEVVGGDHKGIQLALADGYTWLCLPSVCRKDSGRYSVTLRSEGGCVQAEFTLQVIDKPQPPQGPLEVQDCHRAGVCLRWLPPRDNGGQVIQHYVVERRQAGRSTWLKVGEPPSDSTSFTDTSVEQGKKYAFRVRAVTSVGAGDALESEEVLVAPEALPGPPSAPAILSASSQSITLTWGAPQGPGSNHILGYLIEKHKKGSNTWMAVNEQPVSERRYTVVGLRQGCQYEFRVTAVTLSGPGDPGPPSDAVFARDPMRPPGPVRDLQVTDTSNTSITVSWMVPDARDADEAQGYIVELCGSDSHQWSPCHVGTVPGTTFTAKGLRPQEGYFVRVTAVNDGGRSQATSLDTLVHAMPATVCPRFLMDSGTKDTLMVRVGDSIRVPLSFEAAPMPEVTWLKDGLPLPKRSVTTVKAGLTQLLIPAASLSDCGRYTVMLRNLQGKEATHSFFINVAACPQAPGSIYLQENVPGTVTVQWEPSPDEAQGIPLHYTVLMRSSSHRSWHEVADHVRTNRFTLLGVLPGHEYHFRVLAKNELGVSKPSDTSQPWCIPRQRDRFTVKSPTYQDPDLSQKPRFLVGLRAHLLPQGCECRMTCAVQGSPQPHVTWFKNDQSLDRNPAVYSTDLLGVCSLVIPSVSLKDSGEYKAVAKNPLGQAVSTATLIVTEYDS

Target ORF information:

RefSeq Version	XM_017319943.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus immunoglobulin-like and fibronectin type III domain containing 1 (Igfn1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319943.1

ORF Insert Sequence:

ATGGCTGGGA AAAACGCCAT CTTTCGGGCG GTGGTCTGTG GGGAACCCAG ACCTGAAGTG 
CACTGGCAGA GCAGCAAAGG AGACCTCAGC AACTCCGCCA AGTACCAGAT ATCCTCTGCT 
CCCGGTAGAG AGGAGCACGT GCTTCAGATC AACAAGCTGA CTGGAGAGGA TTCGGATCTG 
TACCGCTGCA CTGCACTAAA TGCCTATGGA GAAGCCACAT GCTCAGTGAG GCTTACGGTC 
ATTGAAGTGG GTTTTCGGAA GAACCGAAAA AGGCAAAAGG AACCTCAGGA GGACCTCAGG 
AAGGAGCTGA TGGACTTTCG GAAGATGCTG AAAAAACGGG CCCCACCCCC AACCCCTGAA 
AAAAAGATGG AGTCTGAGCA GGTGTGGCAG CTTTTGGTGA CTGCAGACCG TAAGGACTAC 
GAGAAGATCT GTATGCAGTA TGGTATCGTC GACTTCCGGG GCATGCTGCG CAAGTTGCAG 
GAGATGAAAA AGGAACAGGA GGACAGAATG GCCCAGTATG TCAGTGCCAT TGCCAACTTG 
AGACACATCA AGGTCACCAA GGAAGGGGTC GCTACGTTTG ACCTGGAGCT GGATCTCAAG 
AACCTCGAGA GCAAGATTTA CTTGTACAAG GATGGTGAGA TGATTCCCTA CGGTGTTGAT 
AACCAGACCA AGCACTGTCT GAGACGGCTG GGGAAGCGCT ACCACTTCCA GATCCAGGAC 
CTGCGGCCTG AGGATGCTGG CCTTTACCAG GTCATGGTGG AGGATGCTGT GGTCTTCTCT 
ACAGAGCTAG AATCCAGAAC CATCCCCCCC AGAGTGGTGG TCCCATTGGC AGAGACCCGC 
TGTGAGGAAC GGGGTGATGC AGTCTTTGAA TGTACCCTCT CCAATCCCTG TCCCAGTGCG 
ACCTGGCATT TCCAGCACCG ACCACTGCGG CTCAGTGACA AATATGAAGT ATTTGTGTCC 
CCTGATGGGC TAACCCACCG ACTGGTTGTG AAGGGGGCCA GTAGCTCAGA CATGGGCCTC 
TACTCGCTGA ACACTGGGCT CCATGCCTCC AGTGCCTGGC TGGTGGTTGA AGGTGGGAAG 
GATAAAGGCC CTCAGACCAC AGATACTGAC CACCGGCTGC AGACTCCAGA AGCTCTGGCC 
TCCGAAGCAG AAGACGCTGG AGGCATCAGC ATCAAGGGAG GGCAATCCAG AGAGCGGGGT 
TCCCTCAAAG AGATTAGTGG GGCACAGCTC ACAGCAGGCC CAGACAGGGG TGCCTTTGGC 
AAACATGGCT ATCCCTTGGT GGTGGATGAA GACATAGTTA ATTCTACTTG GGGACCTGGG 
CAGGACAAAA AGAGCTTTCT GGAAGGAGGA CAACTCAGGG TCACTCTTCC AGGGGATAGT 
CAGTCCCAAA GAGAGGGAGA CTGTGGTAGA AGCCTCCCCA GAAGGTCTCA TCTCCAGGGA 
GAGGATACAG AATCAGATTT GGGCTTCCTG GAGAGAGGAC AACAAGGGCC TGGTAGAGTT 
GACAGTGAGA ATGGCAGATG GGAGACAGCA GGAGGCCAGA GTGCTGGCTC CAGCCATCCT 
CGGGACAGAA GGCTTGAGAG CAGAGGAGAA GGACAGGAAC ACAGTGAGGG CCATGGCAGT 
GAACTGGACA GATATGGCCA GGGACAATGC CATGATCCTC AGCTGGGAGC AGGGGCAGGG 
CAGAGGGTGC CGTGGGGATC CCAGTTTGAT GCTGAGGGGC TTCAGCTGAA AAAGGAGGGG 
ACAGAAATGT GGGGCGATTG TCTGGATGTG ACGGAAGGTA GGGGTAACCT GAGAAAGGAG 
AGGGGAACAG CAGCAGGGTC AAGTAACAGA GCCCGCCTCT GGGAAGCTCG AGGAGGCAGT 
GGGGCCGGAA GTCCTTGCGT CCCTGAGTTT CCTAGAGGAG GAGCTCACAG CTCCAAGGCA 
GGCATGGGCC CTGAGTGCTG GCCAGGTGGA GATGCTGGCC ATGGAGAAGC TGGGGTATAC 
TGGGGGGCAG GGGGATATCC AGGACAGACA TCTGAGGGCA ATGACACCCA GAAATCATCA 
CTATCTGGAG ACAGGAAACT TCTGGGGTGC ACGAGTCCTG AGGCCAAAGC TGAGGGCTCT 
TTCCAGAGCG CAGATGGTCA TGGGATTGTG GGGAGGGTCT ACAAACCTGG CCCTGAAGGC 
CCAGAAGATC TTAGGAGTCA GAAAAGTGGC CTACAAGAGA TTCAGGGAAG GAATGGTCAA 
GGCTCAGCTG GAGCCTTGGG CAGGACGGTG GAAGGTTCCA GAAGCCTCCA ATTTCCTCAG 
AGCTGGACAG TGGGACAGAG GGAAGCAGGG AAGCCAGGTG GAGCAGAGTA TGAAGACATA 
GGTCCTCAGG ATGACACGTG GTCCAATCTC AGAGAGATGG GAGGCCACTG TGGGTCTGGA 
GTGCTGGCGT CTGGTGGGAG GGAAGGGAGC GTGGGCGGTT CCCAAGTAGC TGCCCTGATG 
ATGTCTAGTC AGAGGGTAGA TGCCAGAAAC CACAGGCTAG TTACATCCCC AGGCCTGGGT 
GTCCAGGGTT CTGGCAGAAC ACTGGGACAC ATGGAAGGAT TAAGAGACCC AGAGGCAGTG 
GGATCCGAAG AAGAGTTCTG GAATGGGTCA GGAAGGTCCC AAGGAAAAGG GTCCAGAGGT 
GGGATGGGCT TAGGAGGTCC AGAGTGGGCA GAGTCTAGGA ATGAGGAGGG ATGGGAGAAT 
TCTGAAGGGA TGGCTTCTAG GAATGGAGCC ACTTGTCGTG ATGGATCCAG GATGCAGGAA 
GGAACAGGAT CTGGCCCTGA TATTGGTTGC AAGGCCCCCT GTGGGGCACC TGCGGAGACA 
GAGTCTGGGA AGTGGGGTAC TTACAGTCAT GACTCAGGGG TGCCTGGGGG ATTGTGGTCT 
GGAAGCAAAA GTCAGTGTAG TCCTGCAGGA AAGGTATCTG GAAGAGAGGC CAGCCTCAGA 
AATGGCTCAG CTGGCCTTCA TGGAATGCCT TCTAGTGAAG CTCAGCAGAG GGATGTATTT 
CAAGGCCACG GTCAGACAGG CCACATTGGA GAGCACCACT GTGCCCGTGG CTTAGGGAGT 
TCTGAGACAG TGGGATCTGT GGGTGGACAT GGTAGGAAAG ACTCTGGAAC AGCGGGCAAG 
GTTGGAGAGG GATACACAGA AGCAGAACCA GGGCACTCTG GAGGGTTGAG TTCTTGGGGT 
CACACTGGAG ATTATGAGGA CTTCCGAGTC CTGGGGGCCT TTAGGGAAGG AGACTTTGGC 
AATGGCACTG GGGTTCCATT ATCCATGGGA CCAAGGTCTC TGGAGAGAGG GGACAAGGAA 
GGAGATGGAG AAAGGATCAG TTTTCTTGGT GCCAGGACCT CTACAGTTGG AACTGGGAAT 
TGGGACAAAG CCAGGCACCC TGGGGCACCA TCTCCTCATG AGGCCGGTTC AGAGGGCCAC 
TGGGCTCAAG TGTCTGGCAA TGCATTAGGT TGTAGAGATG GGTCTGGAAT TCCAGAGCCT 
TGGAGTGCTG GCGACAAAAC AGCTTATGGA GAAGAATCAA AGGGTCTTGG GCCTGAAAGA 
ACAGGACCAG ATGGTGAGGT GGCATTTAGA GGTAGCTCTT CGAGCCTCAG AGGAATGGGA 
CCAGCAAGTG AGGCAAGTTA TAAAAGTGGA ACTTGGGGCC CTGGGGCTGT GGGTTCAGGG 
TTTAAGGTAG ATTGTAGGAA TGATCTTGGG TGTTCTGGAA GTATGGGGTC AGAGAGTAAA 
GCAGGTTATA AGAACAAAAT TGAGAGTCAT GGGGTCAGGG AGTTAGGGGA CCTGACAGGC 
TATAAAAATG ATTTAGGACC TTCTGAACTT ATTTCATCAA GGAATGAGGC ACGTGGTTTA 
GAACATTCTG GAGACATTTT GTCATGGAAT AAGGCAGGTT TAAGGGATGG CTTGGGAGAA 
ACAGGAAGAA TGGAGTCCAA GAATGGGGTT GGTTATAGGG GAAGCTCAGT GGGTCCTGGG 
GAAATGGGGT CAGAGAGGAA AATGCACCTT GCAGATGGTT CAGGGAGGCT AGGAGGACCA 
GGGTCACTGG CTGCGCCCAA AGAACATGAA TCTGTGGGTC TTGGGTCAGT TTATGAGGGA 
GCAGCAGAGA TTGGCTCCAA ATATAATAGG GAGAAAGTCA GAGGAATGGG GTTTAGAGAT 
GCAACAGGGC CTGAGGTGGG CTCTGGGGTA GCTGGAATGC TGGATATTAC AGGTGGCATA 
GCACACGGGG ACAGTGCTAT GGGCACAGAG AAGCTGGGGA CACCAGATAG GCCTTATATC 
TTGTCAGGTG GGCAGGGTAC CAAGAACAGT CTTGGGGGCT GTGTGTCCTT GGGAATCCCT 
GACGCCTTGG GGGCTGTTGG CTCTGTGGGG AAACCAGGTA TCAGAGAATG GAAAGATGAT 
TCTGGGTTCC AAGGGTCTCT CAGGGACAGA GGGACTCCGA GTGAGGAGAT CAGATCTGTA 
GACCAAGCAG GAGCTATAGG GACATCTCGG CTTCTTGATA GTAGGGGGGC AGTGGAGGAT 
GGGAGTGGGT CAGAAAATGA CTTTGACTTT AATAAATCAG GCTTGGAGAC AACAGGAAGA 
TGTAGAGTTG CTGACCAGCA GGGAGTGACA CCACAGGGAT GTGGAGGCTC ATTGCTCAAT 
GGCAGGAGGA AGGGAACAAG TTCTGGAAGC TTGTCAGGGG TAGGGCAGGA GGTAGACAGA 
AGTTCTACAC CTGGGAGGGA GACCAAGGTA ATGTCAGGAC CTGGCAGTGG GCGAGCCACA 
AGCCACACTC GGGCTGCAGA CTGGGAAGAC GGGGTTCAAT GCAGCTTCGG GGCTTCTAGC 
TCACTGCCTG ATACTGATGC CATTTTCAGA GAAACCCATG CGGGACAAGG AGCCTTCAAG 
GGCAGGGGCT GTGAAACCGG CCAAGGGGCT GCTGGAGAGT GTCAAGGACC ACGCTCCTTG 
GGGAGCAGAG GCTCAGAGTT TGTAGGGGGC AGGGCTGGAT TTACAGGGAG CTCAAGTGTT 
CCAGGCAGAA GGGATTCCAC TATCTACGGA GATGCTGTCA CATCAAAGCC ACAGGAACCC 
CAGAATGAAT TATATAGTCC TTTAGGCAGA AGGGACTTCA AAAGTGTATC GGGAGGAATC 
CAGGAGCCAG GTTTTCAACG AGGAACAGGA CAGGGAGAAG GAAAAGAATC CTTCCAGGAG 
TCAGGGTCCT GGGAGGCTGA GCTTGACGAG GTCCAAGGAT CCAGGGCCTC AGCAAAGTTT 
GAAAACCAGG GAGGGAAAGA CCCTGGCTGG TCAGGCCAGA AGCCTGGTCC CTGTGGAAGC 
AGGCATCAGG TGCAGTCTGG GACTGAAGTT GGATCAGCAA AGAGAGCAAC CCCACAGGGG 
GCCGGAGGGC TGGAGCCTTG GGCTGGCAGT GAGGATAGAG GGTCTCTGAG AGAACCTTGG 
AGTGAAGACA GGAGGCAAGG CCCCCACAGG CACCTTGGCA GCAGGAGAGA TACCCAAGAG 
GGCAGGTCAG ATGTCTGTGG CCAGGCACAA GATGCTACCC AGAGCCCCAG ATCCAGATAC 
CAGCCTGGCA CTGGCCGCTC TTCTTCAGAG GCCCGAGGCT CCATGGACCA CTTCTCTCAG 
GGTCTGACTG ATACAGAGGT GCAACTGGGG GAAGCCGCCA TAGTCTCCTG TACTCTTGCC 
AGTGACCTGG GACCTGGCAC GTGGTTCAAG GATGGAGTCA AGCTCACTGC CCAAGATGGA 
GTCATCTTTG AGCAAGATGG GCTCACGCAC AGACTGATTC TCACCCACGT GGAGGGGACC 
CAGGCTGGGA AATACACTTT TGTAGCCGGC TGCCAGCACA GCGAGGCCAG CCTGACCGTG 
CAGGATCCCC CCACCATTGC TCCAGATGTG ACAGAGACAC TGAGAGAGCC ACTGGTGTTC 
AAGGCTGGGA AGCCAGTCAC CGTGAAAATC CCTTTCCAGA GCCGCCTCCC TGTTCAGGCT 
GCCTGGAGGA AAGATGGGAA CGAGGTGGTG GGCGGCGACC ACAAGGGCAT CCAACTGGCT 
CTGGCAGATG GCTACACATG GCTGTGCCTC CCCAGCGTGT GCAGGAAGGA CAGTGGCCGG 
TACAGCGTGA CGCTGAGGAG CGAGGGAGGC TGTGTGCAGG CTGAGTTCAC CCTGCAAGTT 
ATAGACAAGC CTCAGCCCCC ACAAGGACCC CTGGAGGTTC AGGATTGCCA CAGAGCAGGT 
GTCTGCCTCC GCTGGCTACC CCCGAGGGAC AACGGGGGCC AGGTCATACA ACACTATGTG 
GTGGAGAGGC GACAGGCTGG AAGGAGCACT TGGTTGAAGG TGGGCGAGCC CCCATCAGAC 
AGTACCAGCT TCACCGACAC CAGCGTGGAG CAGGGAAAGA AATATGCCTT CCGTGTGCGG 
GCTGTGACCT CAGTGGGAGC CGGAGATGCC CTGGAGTCAG AGGAGGTGTT GGTGGCTCCT 
GAGGCTCTCC CGGGGCCCCC TTCTGCCCCA GCCATCCTGT CAGCCTCCAG TCAGAGCATC 
ACTCTCACAT GGGGGGCACC ACAGGGCCCT GGCAGTAACC ACATCCTGGG CTACCTGATC 
GAGAAGCACA AGAAGGGGAG CAACACCTGG ATGGCTGTGA ATGAGCAGCC CGTGTCTGAA 
AGAAGGTACA CTGTGGTGGG TCTGCGACAG GGCTGCCAGT ATGAGTTCCG GGTCACGGCC 
GTGACTCTGT CGGGCCCTGG AGATCCTGGG CCTCCATCGG ATGCTGTCTT TGCTAGAGAC 
CCTATGAGAC CCCCAGGGCC TGTGCGGGAT CTACAGGTTA CAGACACATC AAACACTAGC 
ATCACCGTGA GCTGGATGGT GCCGGATGCC CGGGATGCTG ATGAAGCCCA GGGATATATT 
GTGGAGTTGT GTGGTTCAGA CAGCCATCAA TGGAGCCCAT GTCACGTGGG AACTGTGCCG 
GGCACCACCT TCACAGCCAA GGGGCTTCGG CCGCAAGAAG GCTACTTTGT GCGGGTGACA 
GCAGTTAATG ATGGGGGCCG GAGCCAGGCC ACTTCCCTGG ACACATTGGT TCATGCCATG 
CCGGCCACTG TCTGTCCCAG GTTCCTCATG GACTCCGGCA CAAAGGATAC CCTGATGGTC 
AGGGTTGGGG ACAGTATTCG CGTTCCTCTC TCCTTTGAAG CTGCCCCCAT GCCTGAGGTG 
ACCTGGCTGA AGGACGGCTT GCCCTTGCCC AAAAGAAGTG TGACCACTGT GAAGGCTGGC 
CTCACGCAGC TTCTGATCCC TGCAGCTAGC CTCTCGGACT GTGGCCGATA CACCGTGATG 
CTGAGGAACC TACAGGGGAA GGAAGCTACC CACAGCTTCT TCATCAATGT AGCAGCATGC 
CCACAGGCAC CAGGTTCTAT CTACCTACAG GAGAATGTGC CTGGGACAGT GACGGTCCAG 
TGGGAACCCT CTCCGGATGA GGCTCAGGGC ATCCCCTTGC ATTACACAGT GCTGATGCGG 
TCCTCATCTC ACCGATCCTG GCACGAGGTG GCCGACCATG TCCGTACCAA CCGCTTTACC 
CTCCTAGGGG TCCTCCCTGG CCATGAGTAC CACTTTCGGG TGCTGGCCAA GAATGAGCTG 
GGGGTCAGCA AACCTTCAGA CACCAGCCAA CCCTGGTGTA TCCCCAGGCA GAGAGACAGG 
TTCACAGTGA AGTCTCCGAC ATACCAGGAT CCTGACCTGA GCCAGAAGCC CCGCTTCCTG 
GTGGGTCTGC GGGCTCACCT TCTGCCCCAG GGCTGTGAGT GCCGCATGAC GTGTGCCGTG 
CAGGGCTCAC CCCAGCCTCA TGTCACCTGG TTCAAGAATG ACCAAAGCTT GGACAGAAAC 
CCTGCGGTGT ACAGTACTGA CTTGCTGGGT GTGTGCTCCC TCGTCATCCC CAGCGTGTCC 
CTTAAGGACA GTGGTGAATA CAAGGCTGTG GCCAAGAACC CACTGGGACA AGCTGTCAGC 
ACAGCCACCC TCATTGTCAC AGAATACGAC TCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Igfn1 ( XM_006529440.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529503.1 Mus musculus immunoglobulin-like and fibronectin type III domain containing 1 (Igfn1), transcript variant X7, mRNA.

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CloneID	OMu28131
Clone ID Related Accession (Same CDS sequence)	XM_006529440.3
Accession Version	XM_006529440.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6480bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	immunoglobulin-like and fibronectin type III domain-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006529440.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCAA AGCCCCCTAA GAAGTCCTCC ATCCCAGGAG TGAGCATCAG GCAGCTGGTG 
GACGAGATCC CAGAAGGCTG CAGCACACCA GATTTCAAGC AGAAGCCTGT TACCTTGGCT 
CTACCAGAGG GGAAAAACGC CATCTTTCGG GCGGTGGTCT GTGGGGAACC CAGACCTGAA 
GTGCACTGGC AGAGCAGCAA AGGAGACCTC AGCAACTCCG CCAAGTACCA GATATCCTCT 
GCTCCCGGTA GAGAGGAGCA CGTGCTTCAG ATCAACAAGC TGACTGGAGA GGATTCGGAT 
CTGTACCGCT GCACTGCACT AAATGCCTAT GGAGAAGCCA CATGCTCAGT GAGGCTTACG 
GTCATTGAAG TGGGTTTTCG GAAGAACCGA AAAAGGCAAA AGGAACCTCA GGAGGACCTC 
AGGAAGGAGC TGATGGACTT TCGGAAGATG CTGAAAAAAC GGGCCCCACC CCCAACCCCT 
GAAAAAAAGA TGGAGTCTGA GCAGGTGTGG CAGCTTTTGG TGACTGCAGA CCGTAAGGAC 
TACGAGAAGA TCTGTATGCA GTATGGTATC GTCGACTTCC GGGGCATGCT GCGCAAGTTG 
CAGGAGATGA AAAAGGAACA GGAGGACAGA ATGGCCCAGT ATGTCAGTGC CATTGCCAAC 
TTGAGACACA TCAAGGTCAC CAAGGAAGGG GTCGCTACGT TTGACCTGGA GCTGGATCTC 
AAGAACCTCG AGAGCAAGAT TTACTTGTAC AAGGATGGTG AGATGATTCC CTACGGTGTT 
GATAACCAGA CCAAGCACTG TCTGAGACGG CTGGGGAAGC GCTACCACTT CCAGATCCAG 
GACCTGCGGC CTGAGGATGC TGGCCTTTAC CAGGTCATGG TGGAGGATGC TGTGGTCTTC 
TCTACAGAGC TAGAATCCAG AACCATCCCC CCCAGAGTGG TGGTCCCATT GGCAGAGACC 
CGCTGTGAGG AACGGGGTGA TGCAGTCTTT GAATGTACCC TCTCCAATCC CTGTCCCAGT 
GCGACCTGGC ATTTCCAGCA CCGACCACTG CGGCTCAGTG ACAAATATGA AGTATTTGTG 
TCCCCTGATG GGCTAACCCA CCGACTGGTT GTGAAGGGGG CCAGTAGCTC AGACATGGGC 
CTCTACTCGC TGAACACTGG GCTCCATGCC TCCAGTGCCT GGCTGGTGGT TGAAGGTGGG 
AAGGATAAAG GCCCTCAGAC CACAGATACT GACCACCGGC TGCAGACTCC AGAAGCTCTG 
GCCTCCGAAG CAGAAGACGC TGGAGGCATC AGCATCAAGG GAGGGCAATC CAGAGAGCGG 
GGTTCCCTCA AAGAGATTAG TGGGGCACAG CTCACAGCAG GCCCAGACAG GGGTGCCTTT 
GGCAAACATG GCTATCCCTT GGTGGTGGAT GAAGACATAG TTAATTCTAC TTGGGGACCT 
GGGCAGGACA AAAAGAGCTT TCTGGAAGGA GGACAACTCA GGGTCACTCT TCCAGGGGAT 
AGTCAGTCCC AAAGAGAGGG AGACTGTGGT AGAAGCCTCC CCAGAAGGTC TCATCTCCAG 
GGAGAGGATA CAGAATCAGA TTTGGGCTTC CTGGAGAGAG GACAACAAGG GCCTGGTAGA 
GTTGACAGTG AGAATGGCAG ATGGGAGACA GCAGGAGGCC AGAGTGCTGG CTCCAGCCAT 
CCTCGGGACA GAAGGCTTGA GAGCAGAGGA GAAGGACAGG AACACAGTGA GGGCCATGGC 
AGTGAACTGG ACAGATATGG CCAGGGACAA TGCCATGATC CTCAGCTGGG AGCAGGGGCA 
GGGCAGAGGG TGCCGTGGGG ATCCCAGTTT GATGCTGAGG GGCTTCAGCT GAAAAAGGAG 
GGGACAGAAA TGTGGGGCGA TTGTCTGGAT GTGACGGAAG GTAGGGGTAA CCTGAGAAAG 
GAGAGGGGAA CAGCAGCAGG GTCAAGTAAC AGAGCCCGCC TCTGGGAAGC TCGAGGAGGC 
AGTGGGGCCG GAAGTCCTTG CGTCCCTGAG TTTCCTAGAG GAGGAGCTCA CAGCTCCAAG 
GCAGGCATGG GCCCTGAGTG CTGGCCAGGT GGAGATGCTG GCCATGGAGA AGCTGGGGTA 
TACTGGGGGG CAGGGGGATA TCCAGGACAG ACATCTGAGG GCAATGACAC CCAGAAATCA 
TCACTATCTG GAGACAGGAA ACTTCTGGGG TGCACGAGTC CTGAGGCCAA AGCTGAGGGC 
TCTTTCCAGA GCGCAGATGG TCATGGGATT GTGGGGAGGG TCTACAAACC TGGCCCTGAA 
GGCCCAGAAG ATCTTAGGAG TCAGAAAAGT GGCCTACAAG AGATTCAGGG AAGGAATGGT 
CAAGGCTCAG CTGGAGCCTT GGGCAGGACG GTGGAAGGTT CCAGAAGCCT CCAATTTCCT 
CAGAGCTGGA CAGTGGGACA GAGGGAAGCA GGGAAGCCAG GTGGAGCAGA GTATGAAGAC 
ATAGGTCCTC AGGATGACAC GTGGTCCAAT CTCAGAGAGA TGGGAGGCCA CTGTGGGTCT 
GGAGTGCTGG CGTCTGGTGG GAGGGAAGGG AGCGTGGGCG GTTCCCAAGT AGCTGCCCTG 
ATGATGTCTA GTCAGAGGGT AGATGCCAGA AACCACAGGC TAGTTACATC CCCAGGCCTG 
GGTGTCCAGG GTTCTGGCAG AACACTGGGA CACATGGAAG GATTAAGAGA CCCAGAGGCA 
GTGGGATCCG AAGAAGAGTT CTGGAATGGG TCAGGAAGGT CCCAAGGAAA AGGGTCCAGA 
GGTGGGATGG GCTTAGGAGG TCCAGAGTGG GCAGAGTCTA GGAATGAGGA GGGATGGGAG 
AATTCTGAAG GGATGGCTTC TAGGAATGGA GCCACTTGTC GTGATGGATC CAGGATGCAG 
GAAGGAACAG GATCTGGCCC TGATATTGGT TGCAAGGCCC CCTGTGGGGC ACCTGCGGAG 
ACAGAGTCTG GGAAGTGGGG TACTTACAGT CATGACTCAG GGGTGCCTGG GGGATTGTGG 
TCTGGAAGCA AAAGTCAGTG TAGTCCTGCA GGAAAGGTAT CTGGAAGAGA GGCCAGCCTC 
AGAAATGGCT CAGCTGGCCT TCATGGAATG CCTTCTAGTG AAGCTCAGCA GAGGGATGTA 
TTTCAAGGCC ACGGTCAGAC AGGCCACATT GGAGAGCACC ACTGTGCCCG TGGCTTAGGG 
AGTTCTGAGA CAGTGGGATC TGTGGGTGGA CATGGTAGGA AAGACTCTGG AACAGCGGGC 
AAGGTTGGAG AGGGATACAC AGAAGCAGAA CCAGGGCACT CTGGAGGGTT GAGTTCTTGG 
GGTCACACTG GAGATTATGA GGACTTCCGA GTCCTGGGGG CCTTTAGGGA AGGAGACTTT 
GGCAATGGCA CTGGGGTTCC ATTATCCATG GGACCAAGGT CTCTGGAGAG AGGGGACAAG 
GAAGGAGATG GAGAAAGGAT CAGTTTTCTT GGTGCCAGGA CCTCTACAGT TGGAACTGGG 
AATTGGGACA AAGCCAGGCA CCCTGGGGCA CCATCTCCTC ATGAGGCCGG TTCAGAGGGC 
CACTGGGCTC AAGTGTCTGG CAATGCATTA GGTTGTAGAG ATGGGTCTGG AATTCCAGAG 
CCTTGGAGTG CTGGCGACAA AACAGCTTAT GGAGAAGAAT CAAAGGGTCT TGGGCCTGAA 
AGAACAGGAC CAGATGGTGA GGTGGCATTT AGAGGTAGCT CTTCGAGCCT CAGAGGAATG 
GGACCAGCAA GTGAGGCAAG TTATAAAAGT GGAACTTGGG GCCCTGGGGC TGTGGGTTCA 
GGGTTTAAGG TAGATTGTAG GAATGATCTT GGGTGTTCTG GAAGTATGGG GTCAGAGAGT 
AAAGCAGGTT ATAAGAACAA AATTGAGAGT CATGGGGTCA GGGAGTTAGG GGACCTGACA 
GGCTATAAAA ATGATTTAGG ACCTTCTGAA CTTATTTCAT CAAGGAATGA GGCACGTGGT 
TTAGAACATT CTGGAGACAT TTTGTCATGG AATAAGGCAG GTTTAAGGGA TGGCTTGGGA 
GAAACAGGAA GAATGGAGTC CAAGAATGGG GTTGGTTATA GGGGAAGCTC AGTGGGTCCT 
GGGGAAATGG GGTCAGAGAG GAAAATGCAC CTTGCAGATG GTTCAGGGAG GCTAGGAGGA 
CCAGGGTCAC TGGCTGCGCC CAAAGAACAT GAATCTGTGG GTCTTGGGTC AGTTTATGAG 
GGAGCAGCAG AGATTGGCTC CAAATATAAT AGGGAGAAAG TCAGAGGAAT GGGGTTTAGA 
GATGCAACAG GGCCTGAGGT GGGCTCTGGG GTAGCTGGAA TGCTGGATAT TACAGGTGGC 
ATAGCACACG GGGACAGTGC TATGGGCACA GAGAAGCTGG GGACACCAGA TAGGCCTTAT 
ATCTTGTCAG GTGGGCAGGG TACCAAGAAC AGTCTTGGGG GCTGTGTGTC CTTGGGAATC 
CCTGACGCCT TGGGGGCTGT TGGCTCTGTG GGGAAACCAG GTATCAGAGA ATGGAAAGAT 
GATTCTGGGT TCCAAGGGTC TCTCAGGGAC AGAGGGACTC CGAGTGAGGA GATCAGATCT 
GTAGACCAAG CAGGAGCTAT AGGGACATCT CGGCTTCTTG ATAGTAGGGG GGCAGTGGAG 
GATGGGAGTG GGTCAGAAAA TGACTTTGAC TTTAATAAAT CAGGCTTGGA GACAACAGGA 
AGATGTAGAG TTGCTGACCA GCAGGGAGTG ACACCACAGG GATGTGGAGG CTCATTGCTC 
AATGGCAGGA GGAAGGGAAC AAGTTCTGGA AGCTTGTCAG GGGTAGGGCA GGAGGTAGAC 
AGAAGTTCTA CACCTGGGAG GGAGACCAAG GTAATGTCAG GACCTGGCAG TGGGCGAGCC 
ACAAGCCACA CTCGGGCTGC AGACTGGGAA GACGGGGTTC AATGCAGCTT CGGGGCTTCT 
AGCTCACTGC CTGATACTGA TGCCATTTTC AGAGAAACCC ATGCGGGACA AGGAGCCTTC 
AAGGGCAGGG GCTGTGAAAC CGGCCAAGGG GCTGCTGGAG AGTGTCAAGG ACCACGCTCC 
TTGGGGAGCA GAGGCTCAGA GTTTGTAGGG GGCAGGGCTG GATTTACAGG GAGCTCAAGT 
GTTCCAGGCA GAAGGGATTC CACTATCTAC GGAGATGCTG TCACATCAAA GCCACAGGAA 
CCCCAGAATG AATTATATAG TCCTTTAGGC AGAAGGGACT TCAAAAGTGT ATCGGGAGGA 
ATCCAGGAGC CAGGTTTTCA ACGAGGAACA GGACAGGGAG AAGGAAAAGA ATCCTTCCAG 
GAGTCAGGGT CCTGGGAGGC TGAGCTTGAC GAGGTCCAAG GATCCAGGGC CTCAGCAAAG 
TTTGAAAACC AGGGAGGGAA AGACCCTGGC TGGTCAGGCC AGAAGCCTGG TCCCTGTGGA 
AGCAGGCATC AGGTGCAGTC TGGGACTGAA GTTGGATCAG CAAAGAGAGC AACCCCACAG 
GGGGCCGGAG GGCTGGAGCC TTGGGCTGGC AGTGAGGATA GAGGGTCTCT GAGAGAACCT 
TGGAGTGAAG ACAGGAGGCA AGGCCCCCAC AGGCACCTTG GCAGCAGGAG AGATACCCAA 
GAGGGCAGGT CAGATGTCTG TGGCCAGGCA CAAGATGCTA CCCAGAGCCC CAGATCCAGA 
TACCAGCCTG GCACTGGCCG CTCTTCTTCA GAGGCCCGAG GCTCCATGGA CCACTTCTCT 
CAGGGTCTGA CTGATACAGA GGTGCAACTG GGGGAAGCCG CCATAGTCTC CTGTACTCTT 
GCCAGTGACC TGGGACCTGG CACGTGGTTC AAGGATGGAG TCAAGCTCAC TGCCCAAGAT 
GGAGTCATCT TTGAGCAAGA TGGGCTCACG CACAGACTGA TTCTCACCCA CGTGGAGGGG 
ACCCAGGCTG GGAAATACAC TTTTGTAGCC GGCTGCCAGC ACAGCGAGGC CAGCCTGACC 
GTGCAGGATC CCCCCACCAT TGCTCCAGAT GTGACAGAGA CACTGAGAGA GCCACTGGTG 
TTCAAGGCTG GGAAGCCAGT CACCGTGAAA ATCCCTTTCC AGAGCCGCCT CCCTGTTCAG 
GCTGCCTGGA GGAAAGATGG GAACGAGGTG GTGGGCGGCG ACCACAAGGG CATCCAACTG 
GCTCTGGCAG ATGGCTACAC ATGGCTGTGC CTCCCCAGCG TGTGCAGGAA GGACAGTGGC 
CGACAAGCCT CAGCCCCCAC AAGGACCCCT GGAGGTTCAG GATTGCCACA GAGCAGGTGT 
CTGCCTCCGC TGGCTACCCC CGAGGGACAA CGGGGGCCAG GTCATACAAC ACTATGTGGT 
GGAGAGGCGA CAGGCTGGAA GGAGCACTTG GTTGAAGGTG GGCGAGCCCC CATCAGACAG 
TACCAGCTTC ACCGACACCA GCGTGGAGCA GGGAAAGAAA TATGCCTTCC GTGTGCGGGC 
TGTGACCTCA GTGGGAGCCG GAGATGCCCT GGAGTCAGAG GAGGTGTTGG TGGCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529503.1
CDS	567..7046
Translation	MAAKPPKKSSIPGVSIRQLVDEIPEGCSTPDFKQKPVTLALPEGKNAIFRAVVCGEPRPEVHWQSSKGDLSNSAKYQISSAPGREEHVLQINKLTGEDSDLYRCTALNAYGEATCSVRLTVIEVGFRKNRKRQKEPQEDLRKELMDFRKMLKKRAPPPTPEKKMESEQVWQLLVTADRKDYEKICMQYGIVDFRGMLRKLQEMKKEQEDRMAQYVSAIANLRHIKVTKEGVATFDLELDLKNLESKIYLYKDGEMIPYGVDNQTKHCLRRLGKRYHFQIQDLRPEDAGLYQVMVEDAVVFSTELESRTIPPRVVVPLAETRCEERGDAVFECTLSNPCPSATWHFQHRPLRLSDKYEVFVSPDGLTHRLVVKGASSSDMGLYSLNTGLHASSAWLVVEGGKDKGPQTTDTDHRLQTPEALASEAEDAGGISIKGGQSRERGSLKEISGAQLTAGPDRGAFGKHGYPLVVDEDIVNSTWGPGQDKKSFLEGGQLRVTLPGDSQSQREGDCGRSLPRRSHLQGEDTESDLGFLERGQQGPGRVDSENGRWETAGGQSAGSSHPRDRRLESRGEGQEHSEGHGSELDRYGQGQCHDPQLGAGAGQRVPWGSQFDAEGLQLKKEGTEMWGDCLDVTEGRGNLRKERGTAAGSSNRARLWEARGGSGAGSPCVPEFPRGGAHSSKAGMGPECWPGGDAGHGEAGVYWGAGGYPGQTSEGNDTQKSSLSGDRKLLGCTSPEAKAEGSFQSADGHGIVGRVYKPGPEGPEDLRSQKSGLQEIQGRNGQGSAGALGRTVEGSRSLQFPQSWTVGQREAGKPGGAEYEDIGPQDDTWSNLREMGGHCGSGVLASGGREGSVGGSQVAALMMSSQRVDARNHRLVTSPGLGVQGSGRTLGHMEGLRDPEAVGSEEEFWNGSGRSQGKGSRGGMGLGGPEWAESRNEEGWENSEGMASRNGATCRDGSRMQEGTGSGPDIGCKAPCGAPAETESGKWGTYSHDSGVPGGLWSGSKSQCSPAGKVSGREASLRNGSAGLHGMPSSEAQQRDVFQGHGQTGHIGEHHCARGLGSSETVGSVGGHGRKDSGTAGKVGEGYTEAEPGHSGGLSSWGHTGDYEDFRVLGAFREGDFGNGTGVPLSMGPRSLERGDKEGDGERISFLGARTSTVGTGNWDKARHPGAPSPHEAGSEGHWAQVSGNALGCRDGSGIPEPWSAGDKTAYGEESKGLGPERTGPDGEVAFRGSSSSLRGMGPASEASYKSGTWGPGAVGSGFKVDCRNDLGCSGSMGSESKAGYKNKIESHGVRELGDLTGYKNDLGPSELISSRNEARGLEHSGDILSWNKAGLRDGLGETGRMESKNGVGYRGSSVGPGEMGSERKMHLADGSGRLGGPGSLAAPKEHESVGLGSVYEGAAEIGSKYNREKVRGMGFRDATGPEVGSGVAGMLDITGGIAHGDSAMGTEKLGTPDRPYILSGGQGTKNSLGGCVSLGIPDALGAVGSVGKPGIREWKDDSGFQGSLRDRGTPSEEIRSVDQAGAIGTSRLLDSRGAVEDGSGSENDFDFNKSGLETTGRCRVADQQGVTPQGCGGSLLNGRRKGTSSGSLSGVGQEVDRSSTPGRETKVMSGPGSGRATSHTRAADWEDGVQCSFGASSSLPDTDAIFRETHAGQGAFKGRGCETGQGAAGECQGPRSLGSRGSEFVGGRAGFTGSSSVPGRRDSTIYGDAVTSKPQEPQNELYSPLGRRDFKSVSGGIQEPGFQRGTGQGEGKESFQESGSWEAELDEVQGSRASAKFENQGGKDPGWSGQKPGPCGSRHQVQSGTEVGSAKRATPQGAGGLEPWAGSEDRGSLREPWSEDRRQGPHRHLGSRRDTQEGRSDVCGQAQDATQSPRSRYQPGTGRSSSEARGSMDHFSQGLTDTEVQLGEAAIVSCTLASDLGPGTWFKDGVKLTAQDGVIFEQDGLTHRLILTHVEGTQAGKYTFVAGCQHSEASLTVQDPPTIAPDVTETLREPLVFKAGKPVTVKIPFQSRLPVQAAWRKDGNEVVGGDHKGIQLALADGYTWLCLPSVCRKDSGRQASAPTRTPGGSGLPQSRCLPPLATPEGQRGPGHTTLCGGEATGWKEHLVEGGRAPIRQYQLHRHQRGAGKEICLPCAGCDLSGSRRCPGVRGGVGGS

Target ORF information:

RefSeq Version	XM_006529440.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus immunoglobulin-like and fibronectin type III domain containing 1 (Igfn1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529440.3

ORF Insert Sequence:

ATGGCTGCAA AGCCCCCTAA GAAGTCCTCC ATCCCAGGAG TGAGCATCAG GCAGCTGGTG 
GACGAGATCC CAGAAGGCTG CAGCACACCA GATTTCAAGC AGAAGCCTGT TACCTTGGCT 
CTACCAGAGG GGAAAAACGC CATCTTTCGG GCGGTGGTCT GTGGGGAACC CAGACCTGAA 
GTGCACTGGC AGAGCAGCAA AGGAGACCTC AGCAACTCCG CCAAGTACCA GATATCCTCT 
GCTCCCGGTA GAGAGGAGCA CGTGCTTCAG ATCAACAAGC TGACTGGAGA GGATTCGGAT 
CTGTACCGCT GCACTGCACT AAATGCCTAT GGAGAAGCCA CATGCTCAGT GAGGCTTACG 
GTCATTGAAG TGGGTTTTCG GAAGAACCGA AAAAGGCAAA AGGAACCTCA GGAGGACCTC 
AGGAAGGAGC TGATGGACTT TCGGAAGATG CTGAAAAAAC GGGCCCCACC CCCAACCCCT 
GAAAAAAAGA TGGAGTCTGA GCAGGTGTGG CAGCTTTTGG TGACTGCAGA CCGTAAGGAC 
TACGAGAAGA TCTGTATGCA GTATGGTATC GTCGACTTCC GGGGCATGCT GCGCAAGTTG 
CAGGAGATGA AAAAGGAACA GGAGGACAGA ATGGCCCAGT ATGTCAGTGC CATTGCCAAC 
TTGAGACACA TCAAGGTCAC CAAGGAAGGG GTCGCTACGT TTGACCTGGA GCTGGATCTC 
AAGAACCTCG AGAGCAAGAT TTACTTGTAC AAGGATGGTG AGATGATTCC CTACGGTGTT 
GATAACCAGA CCAAGCACTG TCTGAGACGG CTGGGGAAGC GCTACCACTT CCAGATCCAG 
GACCTGCGGC CTGAGGATGC TGGCCTTTAC CAGGTCATGG TGGAGGATGC TGTGGTCTTC 
TCTACAGAGC TAGAATCCAG AACCATCCCC CCCAGAGTGG TGGTCCCATT GGCAGAGACC 
CGCTGTGAGG AACGGGGTGA TGCAGTCTTT GAATGTACCC TCTCCAATCC CTGTCCCAGT 
GCGACCTGGC ATTTCCAGCA CCGACCACTG CGGCTCAGTG ACAAATATGA AGTATTTGTG 
TCCCCTGATG GGCTAACCCA CCGACTGGTT GTGAAGGGGG CCAGTAGCTC AGACATGGGC 
CTCTACTCGC TGAACACTGG GCTCCATGCC TCCAGTGCCT GGCTGGTGGT TGAAGGTGGG 
AAGGATAAAG GCCCTCAGAC CACAGATACT GACCACCGGC TGCAGACTCC AGAAGCTCTG 
GCCTCCGAAG CAGAAGACGC TGGAGGCATC AGCATCAAGG GAGGGCAATC CAGAGAGCGG 
GGTTCCCTCA AAGAGATTAG TGGGGCACAG CTCACAGCAG GCCCAGACAG GGGTGCCTTT 
GGCAAACATG GCTATCCCTT GGTGGTGGAT GAAGACATAG TTAATTCTAC TTGGGGACCT 
GGGCAGGACA AAAAGAGCTT TCTGGAAGGA GGACAACTCA GGGTCACTCT TCCAGGGGAT 
AGTCAGTCCC AAAGAGAGGG AGACTGTGGT AGAAGCCTCC CCAGAAGGTC TCATCTCCAG 
GGAGAGGATA CAGAATCAGA TTTGGGCTTC CTGGAGAGAG GACAACAAGG GCCTGGTAGA 
GTTGACAGTG AGAATGGCAG ATGGGAGACA GCAGGAGGCC AGAGTGCTGG CTCCAGCCAT 
CCTCGGGACA GAAGGCTTGA GAGCAGAGGA GAAGGACAGG AACACAGTGA GGGCCATGGC 
AGTGAACTGG ACAGATATGG CCAGGGACAA TGCCATGATC CTCAGCTGGG AGCAGGGGCA 
GGGCAGAGGG TGCCGTGGGG ATCCCAGTTT GATGCTGAGG GGCTTCAGCT GAAAAAGGAG 
GGGACAGAAA TGTGGGGCGA TTGTCTGGAT GTGACGGAAG GTAGGGGTAA CCTGAGAAAG 
GAGAGGGGAA CAGCAGCAGG GTCAAGTAAC AGAGCCCGCC TCTGGGAAGC TCGAGGAGGC 
AGTGGGGCCG GAAGTCCTTG CGTCCCTGAG TTTCCTAGAG GAGGAGCTCA CAGCTCCAAG 
GCAGGCATGG GCCCTGAGTG CTGGCCAGGT GGAGATGCTG GCCATGGAGA AGCTGGGGTA 
TACTGGGGGG CAGGGGGATA TCCAGGACAG ACATCTGAGG GCAATGACAC CCAGAAATCA 
TCACTATCTG GAGACAGGAA ACTTCTGGGG TGCACGAGTC CTGAGGCCAA AGCTGAGGGC 
TCTTTCCAGA GCGCAGATGG TCATGGGATT GTGGGGAGGG TCTACAAACC TGGCCCTGAA 
GGCCCAGAAG ATCTTAGGAG TCAGAAAAGT GGCCTACAAG AGATTCAGGG AAGGAATGGT 
CAAGGCTCAG CTGGAGCCTT GGGCAGGACG GTGGAAGGTT CCAGAAGCCT CCAATTTCCT 
CAGAGCTGGA CAGTGGGACA GAGGGAAGCA GGGAAGCCAG GTGGAGCAGA GTATGAAGAC 
ATAGGTCCTC AGGATGACAC GTGGTCCAAT CTCAGAGAGA TGGGAGGCCA CTGTGGGTCT 
GGAGTGCTGG CGTCTGGTGG GAGGGAAGGG AGCGTGGGCG GTTCCCAAGT AGCTGCCCTG 
ATGATGTCTA GTCAGAGGGT AGATGCCAGA AACCACAGGC TAGTTACATC CCCAGGCCTG 
GGTGTCCAGG GTTCTGGCAG AACACTGGGA CACATGGAAG GATTAAGAGA CCCAGAGGCA 
GTGGGATCCG AAGAAGAGTT CTGGAATGGG TCAGGAAGGT CCCAAGGAAA AGGGTCCAGA 
GGTGGGATGG GCTTAGGAGG TCCAGAGTGG GCAGAGTCTA GGAATGAGGA GGGATGGGAG 
AATTCTGAAG GGATGGCTTC TAGGAATGGA GCCACTTGTC GTGATGGATC CAGGATGCAG 
GAAGGAACAG GATCTGGCCC TGATATTGGT TGCAAGGCCC CCTGTGGGGC ACCTGCGGAG 
ACAGAGTCTG GGAAGTGGGG TACTTACAGT CATGACTCAG GGGTGCCTGG GGGATTGTGG 
TCTGGAAGCA AAAGTCAGTG TAGTCCTGCA GGAAAGGTAT CTGGAAGAGA GGCCAGCCTC 
AGAAATGGCT CAGCTGGCCT TCATGGAATG CCTTCTAGTG AAGCTCAGCA GAGGGATGTA 
TTTCAAGGCC ACGGTCAGAC AGGCCACATT GGAGAGCACC ACTGTGCCCG TGGCTTAGGG 
AGTTCTGAGA CAGTGGGATC TGTGGGTGGA CATGGTAGGA AAGACTCTGG AACAGCGGGC 
AAGGTTGGAG AGGGATACAC AGAAGCAGAA CCAGGGCACT CTGGAGGGTT GAGTTCTTGG 
GGTCACACTG GAGATTATGA GGACTTCCGA GTCCTGGGGG CCTTTAGGGA AGGAGACTTT 
GGCAATGGCA CTGGGGTTCC ATTATCCATG GGACCAAGGT CTCTGGAGAG AGGGGACAAG 
GAAGGAGATG GAGAAAGGAT CAGTTTTCTT GGTGCCAGGA CCTCTACAGT TGGAACTGGG 
AATTGGGACA AAGCCAGGCA CCCTGGGGCA CCATCTCCTC ATGAGGCCGG TTCAGAGGGC 
CACTGGGCTC AAGTGTCTGG CAATGCATTA GGTTGTAGAG ATGGGTCTGG AATTCCAGAG 
CCTTGGAGTG CTGGCGACAA AACAGCTTAT GGAGAAGAAT CAAAGGGTCT TGGGCCTGAA 
AGAACAGGAC CAGATGGTGA GGTGGCATTT AGAGGTAGCT CTTCGAGCCT CAGAGGAATG 
GGACCAGCAA GTGAGGCAAG TTATAAAAGT GGAACTTGGG GCCCTGGGGC TGTGGGTTCA 
GGGTTTAAGG TAGATTGTAG GAATGATCTT GGGTGTTCTG GAAGTATGGG GTCAGAGAGT 
AAAGCAGGTT ATAAGAACAA AATTGAGAGT CATGGGGTCA GGGAGTTAGG GGACCTGACA 
GGCTATAAAA ATGATTTAGG ACCTTCTGAA CTTATTTCAT CAAGGAATGA GGCACGTGGT 
TTAGAACATT CTGGAGACAT TTTGTCATGG AATAAGGCAG GTTTAAGGGA TGGCTTGGGA 
GAAACAGGAA GAATGGAGTC CAAGAATGGG GTTGGTTATA GGGGAAGCTC AGTGGGTCCT 
GGGGAAATGG GGTCAGAGAG GAAAATGCAC CTTGCAGATG GTTCAGGGAG GCTAGGAGGA 
CCAGGGTCAC TGGCTGCGCC CAAAGAACAT GAATCTGTGG GTCTTGGGTC AGTTTATGAG 
GGAGCAGCAG AGATTGGCTC CAAATATAAT AGGGAGAAAG TCAGAGGAAT GGGGTTTAGA 
GATGCAACAG GGCCTGAGGT GGGCTCTGGG GTAGCTGGAA TGCTGGATAT TACAGGTGGC 
ATAGCACACG GGGACAGTGC TATGGGCACA GAGAAGCTGG GGACACCAGA TAGGCCTTAT 
ATCTTGTCAG GTGGGCAGGG TACCAAGAAC AGTCTTGGGG GCTGTGTGTC CTTGGGAATC 
CCTGACGCCT TGGGGGCTGT TGGCTCTGTG GGGAAACCAG GTATCAGAGA ATGGAAAGAT 
GATTCTGGGT TCCAAGGGTC TCTCAGGGAC AGAGGGACTC CGAGTGAGGA GATCAGATCT 
GTAGACCAAG CAGGAGCTAT AGGGACATCT CGGCTTCTTG ATAGTAGGGG GGCAGTGGAG 
GATGGGAGTG GGTCAGAAAA TGACTTTGAC TTTAATAAAT CAGGCTTGGA GACAACAGGA 
AGATGTAGAG TTGCTGACCA GCAGGGAGTG ACACCACAGG GATGTGGAGG CTCATTGCTC 
AATGGCAGGA GGAAGGGAAC AAGTTCTGGA AGCTTGTCAG GGGTAGGGCA GGAGGTAGAC 
AGAAGTTCTA CACCTGGGAG GGAGACCAAG GTAATGTCAG GACCTGGCAG TGGGCGAGCC 
ACAAGCCACA CTCGGGCTGC AGACTGGGAA GACGGGGTTC AATGCAGCTT CGGGGCTTCT 
AGCTCACTGC CTGATACTGA TGCCATTTTC AGAGAAACCC ATGCGGGACA AGGAGCCTTC 
AAGGGCAGGG GCTGTGAAAC CGGCCAAGGG GCTGCTGGAG AGTGTCAAGG ACCACGCTCC 
TTGGGGAGCA GAGGCTCAGA GTTTGTAGGG GGCAGGGCTG GATTTACAGG GAGCTCAAGT 
GTTCCAGGCA GAAGGGATTC CACTATCTAC GGAGATGCTG TCACATCAAA GCCACAGGAA 
CCCCAGAATG AATTATATAG TCCTTTAGGC AGAAGGGACT TCAAAAGTGT ATCGGGAGGA 
ATCCAGGAGC CAGGTTTTCA ACGAGGAACA GGACAGGGAG AAGGAAAAGA ATCCTTCCAG 
GAGTCAGGGT CCTGGGAGGC TGAGCTTGAC GAGGTCCAAG GATCCAGGGC CTCAGCAAAG 
TTTGAAAACC AGGGAGGGAA AGACCCTGGC TGGTCAGGCC AGAAGCCTGG TCCCTGTGGA 
AGCAGGCATC AGGTGCAGTC TGGGACTGAA GTTGGATCAG CAAAGAGAGC AACCCCACAG 
GGGGCCGGAG GGCTGGAGCC TTGGGCTGGC AGTGAGGATA GAGGGTCTCT GAGAGAACCT 
TGGAGTGAAG ACAGGAGGCA AGGCCCCCAC AGGCACCTTG GCAGCAGGAG AGATACCCAA 
GAGGGCAGGT CAGATGTCTG TGGCCAGGCA CAAGATGCTA CCCAGAGCCC CAGATCCAGA 
TACCAGCCTG GCACTGGCCG CTCTTCTTCA GAGGCCCGAG GCTCCATGGA CCACTTCTCT 
CAGGGTCTGA CTGATACAGA GGTGCAACTG GGGGAAGCCG CCATAGTCTC CTGTACTCTT 
GCCAGTGACC TGGGACCTGG CACGTGGTTC AAGGATGGAG TCAAGCTCAC TGCCCAAGAT 
GGAGTCATCT TTGAGCAAGA TGGGCTCACG CACAGACTGA TTCTCACCCA CGTGGAGGGG 
ACCCAGGCTG GGAAATACAC TTTTGTAGCC GGCTGCCAGC ACAGCGAGGC CAGCCTGACC 
GTGCAGGATC CCCCCACCAT TGCTCCAGAT GTGACAGAGA CACTGAGAGA GCCACTGGTG 
TTCAAGGCTG GGAAGCCAGT CACCGTGAAA ATCCCTTTCC AGAGCCGCCT CCCTGTTCAG 
GCTGCCTGGA GGAAAGATGG GAACGAGGTG GTGGGCGGCG ACCACAAGGG CATCCAACTG 
GCTCTGGCAG ATGGCTACAC ATGGCTGTGC CTCCCCAGCG TGTGCAGGAA GGACAGTGGC 
CGACAAGCCT CAGCCCCCAC AAGGACCCCT GGAGGTTCAG GATTGCCACA GAGCAGGTGT 
CTGCCTCCGC TGGCTACCCC CGAGGGACAA CGGGGGCCAG GTCATACAAC ACTATGTGGT 
GGAGAGGCGA CAGGCTGGAA GGAGCACTTG GTTGAAGGTG GGCGAGCCCC CATCAGACAG 
TACCAGCTTC ACCGACACCA GCGTGGAGCA GGGAAAGAAA TATGCCTTCC GTGTGCGGGC 
TGTGACCTCA GTGGGAGCCG GAGATGCCCT GGAGTCAGAG GAGGTGTTGG TGGCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Igfn1 ( NM_177642.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_808310.2 Mus musculus immunoglobulin-like and fibronectin type III domain containing 1 (Igfn1), mRNA.

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CloneID	OMu06590
Clone ID Related Accession (Same CDS sequence)	NM_177642.3
Accession Version	NM_177642.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Mus musculus(house mouse)
Product	immunoglobulin-like and fibronectin type III domain-containing protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from GU290042.1 and AC108813.8. On or before Nov 14, 2010 this sequence version replaced XM_001478252.2, XM_001478294.2, NM_177642.2. ##Evidence-Data-START## Transcript exon combination :: GU290042.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN01164131 [ECO:0000350] ##Evidence-Data-END##

ATGAATGAGA TGCGAACACA AGTTAGAATT GAGTTTCATG CTTTGGCAAC TCAAAGGAAG 
CCTTCAGCAT CACCTCCTAC CATTGAGGAG GACATGGGCC AAGGTTGTAC TCTAGAAGCT 
CTGGTCATCC CTAGCAGAAG AGCTGAGCAA TCAACTCAGC ACCCAGACAG CTCATTCATG 
GCCACGGTCA GAGGTGCTAA ATCTGTAGTA GTCAGTCAAT GGCAGCTGCT ACAGGCCGGA 
TCCGCAGGCT CTGCCGCCAG ACCAGACGGC TCCGGCTCTG AGTCCCTGGC AGCCAGCAGC 
AGCTGGAAAC CAAGAAGGAG GCTGGAAGCT GGTGGGCCAT GCAGATCATG GCTGGTGTTT 
CTGGTGTCAC GGGAAGGATG GAGTGATAGC AACCCGGGCT ATCGTAAACA ATTCATCTGT 
CGCCCTCAGG AGATCGGTTT CCACTCAGAG GAGCACAGAG AAGCTGGGAC TATGGCTGCA 
AAGCCCCCTA AGAAGTCCTC CATCCCAGGA GTGAGCATCA GGCAGCTGGT GGACGAGATC 
CCAGAAGGCT GCAGCACACC AGATTTCAAG CAGAAGCCTG TTACCTTGGC TCTACCAGAG 
GGGAAAAACG CCATCTTTCG GGCGGTGGTC TGTGGGGAAC CCAGACCTGA AGTGCACTGG 
CAGAGCAGCA AAGGAGACCT CAGCAACTCC GCCAAGTACC AGATATCCTC TGCTCCCGGT 
AGAGAGGAGC ACGTGCTTCA GATCAACAAG CTGACTGGAG AGGATTCGGA TCTGTACCGC 
TGCACTGCAC TAAATGCCTA TGGAGAAGCC ACATGCTCAG TGAGGCTTAC GGTCATTGAA 
GTGGGTTTTC GGAAGAACCG AAAAAGGCAA AAGGAACCTC AGGAGGACCT CAGGAAGGAG 
CTGATGGACT TTCGGAAGAT GCTGAAAAAA CGGGCCCCAC CCCCAACCCC TGAAAAAAAG 
ATGGAGTCTG AGCAGGTGTG GCAGCTTTTG GTGACTGCAG ACCGTAAGGA CTACGAGAAG 
ATCTGTATGC AGTATGGTAT CGTCGACTTC CGGGGCATGC TGCGCAAGTT GCAGGAGATG 
AAAAAGGAAC AGGAGGACAG AATGGCCCAG TATGTCAGTG CCATTGCCAA CTTGAGACAC 
ATCAAGGTCA CCAAGGAAGG GGTCGCTACG TTTGACCTGG AGCTGGATCT CAAGAACCTC 
GAGAGCAAGA TTTACTTGTA CAAGGATGGT GAGATGATTC CCTACGGTGT TGATAACCAG 
ACCAAGCACT GTCTGAGACG GCTGGGGAAG CGCTACCACT TCCAGATCCA GGACCTGCGG 
CCTGAGGATG CTGGCCTTTA CCAGGTCATG GTGGAGGATG CTGTGGTCTT CTCTACAGAG 
CTAGAATCCA GAACCATCCC CCCCAGAGTG GTGGTCCCAT TGGCAGAGAC CCGCTGTGAG 
GAACGGGGTG ATGCAGTCTT TGAATGTACC CTCTCCAATC CCTGTCCCAG TGCGACCTGG 
CATTTCCAGC ACCGACCACT GCGGCTCAGT GACAAATATG AAGTATTTGT GTCCCCTGAT 
GGGCTAACCC ACCGACTGGT TGTGAAGGGG GCCAGTAGCT CAGACATGGG CCTCTACTCG 
CTGAACACTG GGCTCCATGC CTCCAGTGCC TGGCTGGTGG TTGAAGGTGG GAAGGATAAA 
GGCCCTCAGA CCACAGATAC TGACCACCGG CTGCAGACTC CAGAAGCTCT GGCCTCCGAA 
GCAGAAGACG CTGGAGGCAT CAGCATCAAG GGAGGGCAAT CCAGAGAGCG GGGTTCCCTC 
AAAGAGATTA GTGGGGCACA GCTCACAGCA GGCCCAGACA GGGGTGCCTT TGGCAAACAT 
GGCTATCCCT TGGTGGTGGA TGAAGACATA GTTAATTCTA CTTGGGGACC TGGGCAGGAC 
AAAAAGAGCT TTCTGGAAGG AGGACAACTC AGGGTCACTC TTCCAGGGGA TAGTCAGTCC 
CAAAGAGAGG GAGACTGTGG TAGAAGCCTC CCCAGAAGGT CTCATCTCCA GGGAGAGGAT 
ACAGAATCAG ATTTGGGCTT CCTGGAGAGA GGACAACAAG GGCCTGGTAG AGTTGACAGT 
GAGAATGGCA GATGGGAGAC AGCAGGAGGC CAGAGTGCTG GCTCCAGCCA TCCTCGGGAC 
AGAAGGCTTG AGAGCAGAGG AGAAGGACAG GAACACAGTG AGGGCCATGG CAGTGAACTG 
GACAGATATG GCCAGGGACA ATGCCATGAT CCTCAGCTGG GAGCAGGGGC AGGGCAGAGG 
GTGCCGTGGG GATCCCAGTT TGATGCTGAG GGGCTTCAGC TGAAAAAGGA GGGGACAGAA 
ATGTGGGGCG ATTGTCTGGA TGTGACGGAA GGTAGGGGTA ACCTGAGAAA GGAGAGGGGA 
ACAGCAGCAG GGTCAAGTAA CAGAGCCCGC CTCTGGGAAG CTCGAGGAGG CAGTGGGGCC 
GGAAGTCCTT GCGTCCCTGA GTTTCCTAGA GGAGGAGCTC ACAGCTCCAA GGCAGGCATG 
GGCCCTGAGT GCTGGCCAGG TGGAGATGCT GGCCATGGAG AAGCTGGGGT ATACTGGGGG 
GCAGGGGGAT ATCCAGGACA GACATCTGAG GGCAATGACA CCCAGAAATC ATCACTATCT 
GGAGACAGGA AACTTCTGGG GTGCACGAGT CCTGAGGCCA AAGCTGAGGG CTCTTTCCAG 
AGCGCAGATG GTCATGGGAT TGTGGGGAGG GTCTACAAAC CTGGCCCTGA AGGCCCAGAA 
GATCTTAGGA GTCAGAAAAG TGGCCTACAA GAGATTCAGG GAAGGAATGG TCAAGGCTCA 
GCTGGAGCCT TGGGCAGGAC GGTGGAAGGT TCCAGAAGCC TCCAATTTCC TCAGAGCTGG 
ACAGTGGGAC AGAGGGAAGC AGGGAAGCCA GGTGGAGCAG AGTATGAAGA CATAGGTCCT 
CAGGATGACA CGTGGTCCAA TCTCAGAGAG ATGGGAGGCC ACTGTGGGTC TGGAGTGCTG 
GCGTCTGGTG GGAGGGAAGG GAGCGTGGGC GGTTCCCAAG TAGCTGCCCT GATGATGTCT 
AGTCAGAGGG TAGATGCCAG AAACCACAGG CTAGTTACAT CCCCAGGCCT GGGTGTCCAG 
GGTTCTGGCA GAACACTGGG ACACATGGAA GGATTAAGAG ACCCAGAGGC AGTGGGATCC 
GAAGAAGAGT TCTGGAATGG GTCAGGAAGG TCCCAAGGAA AAGGGTCCAG AGGTGGGATG 
GGCTTAGGAG GTCCAGAGTG GGCAGAGTCT AGGAATGAGG AGGGATGGGA GAATTCTGAA 
GGGATGGCTT CTAGGAATGG AGCCACTTGT CGTGATGGAT CCAGGATGCA GGAAGGAACA 
GGATCTGGCC CTGATATTGG TTGCAAGGCC CCCTGTGGGG CACCTGCGGA GACAGAGTCT 
GGGAAGTGGG GTACTTACAG TCATGACTCA GGGGTGCCTG GGGGATTGTG GTCTGGAAGC 
AAAAGTCAGT GTAGTCCTGC AGGAAAGGTA TCTGGAAGAG AGGCCAGCCT CAGAAATGGC 
TCAGCTGGCC TTCATGGAAT GCCTTCTAGT GAAGCTCAGC AGAGGGATGT ATTTCAAGGC 
CACGGTCAGA CAGGCCACAT TGGAGAGCAC CACTGTGCCC GTGGCTTAGG GAGTTCTGAG 
ACAGTGGGAT CTGTGGGTGG ACATGGTAGG AAAGACTCTG GAACAGCGGG CAAGGTTGGA 
GAGGGATACA CAGAAGCAGA ACCAGGGCAC TCTGGAGGGT TGAGTTCTTG GGGTCACACT 
GGAGATTATG AGGACTTCCG AGTCCTGGGG GCCTTTAGGG AAGGAGACTT TGGCAATGGC 
ACTGGGGTTC CATTATCCAT GGGACCAAGG TCTCTGGAGA GAGGGGACAA GGAAGGAGAT 
GGAGAAAGGA TCAGTTTTCT TGGTGCCAGG ACCTCTACAG TTGGAACTGG GAATTGGGAC 
AAAGCCAGGC ACCCTGGGGC ACCATCTCCT CATGAGGCCG GTTCAGAGGG CCACTGGGCT 
CAAGTGTCTG GCAATGCATT AGGTTGTAGA GATGGGTCTG GAATTCCAGA GCCTTGGAGT 
GCTGGCGACA AAACAGCTTA TGGAGAAGAA TCAAAGGGTC TTGGGCCTGA AAGAACAGGA 
CCAGATGGTG AGGTGGCATT TAGAGGTAGC TCTTCGAGCC TCAGAGGAAT GGGACCAGCA 
AGTGAGGCAA GTTATAAAAG TGGAACTTGG GGCCCTGGGG CTGTGGGTTC AGGGTTTAAG 
GTAGATTGTA GGAATGATCT TGGGTGTTCT GGAAGTATGG GGTCAGAGAG TAAAGCAGGT 
TATAAGAACA AAATTGAGAG TCATGGGGTC AGGGAGTTAG GGGACCTGAC AGGCTATAAA 
AATGATTTAG GACCTTCTGA ACTTATTTCA TCAAGGAATG AGGCACGTGG TTTAGAACAT 
TCTGGAGACA TTTTGTCATG GAATAAGGCA GGTTTAAGGG ATGGCTTGGG AGAAACAGGA 
AGAATGGAGT CCAAGAATGG GGTTGGTTAT AGGGGAAGCT CAGTGGGTCC TGGGGAAATG 
GGGTCAGAGA GGAAAATGCA CCTTGCAGAT GGTTCAGGGA GGCTAGGAGG ACCAGGGTCA 
CTGGCTGCGC CCAAAGAACA TGAATCTGTG GGTCTTGGGT CAGTTTATGA GGGAGCAGCA 
GAGATTGGCT CCAAATATAA TAGGGAGAAA GTCAGAGGAA TGGGGTTTAG AGATGCAACA 
GGGCCTGAGG TGGGCTCTGG GGTAGCTGGA ATGCTGGATA TTACAGGTGG CATAGCACAC 
GGGGACAGTG CTATGGGCAC AGAGAAGCTG GGGACACCAG ATAGGCCTTA TATCTTGTCA 
GGTGGGCAGG GTACCAAGAA CAGTCTTGGG GGCTGTGTGT CCTTGGGAAT CCCTGACGCC 
TTGGGGGCTG TTGGCTCTGT GGGGAAACCA GGTATCAGAG AATGGAAAGA TGATTCTGGG 
TTCCAAGGGT CTCTCAGGGA CAGAGGGACT CCGAGTGAGG AGATCAGATC TGTAGACCAA 
GCAGGAGCTA TAGGGACATC TCGGCTTCTT GATAGTAGGG GGGCAGTGGA GGATGGGAGT 
GGGTCAGAAA ATGACTTTGA CTTTAATAAA TCAGGCTTGG AGACAACAGG AAGATGTAGA 
GTTGCTGACC AGCAGGGAGT GACACCACAG GGATGTGGAG GCTCATTGCT CAATGGCAGG 
AGGAAGGGAA CAAGTTCTGG AAGCTTGTCA GGGGTAGGGC AGGAGGTAGA CAGAAGTTCT 
ACACCTGGGA GGGAGACCAA GGTAATGTCA GGACCTGGCA GTGGGCGAGC CACAAGCCAC 
ACTCGGGCTG CAGACTGGGA AGACGGGGTT CAATGCAGCT TCGGGGCTTC TAGCTCACTG 
CCTGATACTG ATGCCATTTT CAGAGAAACC CATGCGGGAC AAGGAGCCTT CAAGGGCAGG 
GGCTGTGAAA CCGGCCAAGG GGCTGCTGGA GAGTGTCAAG GACCACGCTC CTTGGGGAGC 
AGAGGCTCAG AGTTTGTAGG GGGCAGGGCT GGATTTACAG GGAGCTCAAG TGTTCCAGGC 
AGAAGGGATT CCACTATCTA CGGAGATGCT GTCACATCAA AGCCACAGGA ACCCCAGAAT 
GAATTATATA GTCCTTTAGG CAGAAGGGAC TTCAAAAGTG TATCGGGAGG AATCCAGGAG 
CCAGGTTTTC AACGAGGAAC AGGACAGGGA GAAGGAAAAG AATCCTTCCA GGAGTCAGGG 
TCCTGGGAGG CTGAGCTTGA CGAGGTCCAA GGATCCAGGG CCTCAGCAAA GTTTGAAAAC 
CAGGGAGGGA AAGACCCTGG CTGGTCAGGC CAGAAGCCTG GTCCCTGTGG AAGCAGGCAT 
CAGGTGCAGT CTGGGACTGA AGTTGGATCA GCAAAGAGAG CAACCCCACA GGGGGCCGGA 
GGGCTGGAGC CTTGGGCTGG CAGTGAGGAT AGAGGGTCTC TGAGAGAACC TTGGAGTGAA 
GACAGGAGGC AAGGCCCCCA CAGGCACCTT GGCAGCAGGA GAGATACCCA AGAGGGCAGG 
TCAGATGTCT GTGGCCAGGC ACAAGATGCT ACCCAGAGCC CCAGATCCAG ATACCAGCCT 
GGCACTGGCC GCTCTTCTTC AGAGGCCCGA GGCTCCATGG ACCACTTCTC TCAGGGTCTG 
ACTGATACAG AGGTGCAACT GGGGGAAGCC GCCATAGTCT CCTGTACTCT TGCCAGTGAC 
CTGGGACCTG GCACGTGGTT CAAGGATGGA GTCAAGCTCA CTGCCCAAGA TGGAGTCATC 
TTTGAGCAAG ATGGGCTCAC GCACAGACTG ATTCTCACCC ACGTGGAGGG GACCCAGGCT 
GGGAAATACA CTTTTGTAGC CGGCTGCCAG CACAGCGAGG CCAGCCTGAC CGTGCAGGAT 
CCCCCCACCA TTGCTCCAGA TGTGACAGAG ACACTGAGAG AGCCACTGGT GTTCAAGGCT 
GGGAAGCCAG TCACCGTGAA AATCCCTTTC CAGAGCCGCC TCCCTGTTCA GGCTGCCTGG 
AGGAAAGATG GGAACGAGGT GGTGGGCGGC GACCACAAGG GCATCCAACT GGCTCTGGCA 
GATGGCTACA CATGGCTGTG CCTCCCCAGC GTGTGCAGGA AGGACAGTGG CCGGTACAGC 
GTGACGCTGA GGAGCGAGGG AGGCTGTGTG CAGGCTGAGT TCACCCTGCA AGTTATAGAC 
AAGCCTCAGC CCCCACAAGG ACCCCTGGAG GTTCAGGATT GCCACAGAGC AGGTGTCTGC 
CTCCGCTGGC TACCCCCGAG GGACAACGGG GGCCAGGTCA TACAACACTA TGTGGTGGAG 
AGGCGACAGG CTGGAAGGAG CACTTGGTTG AAGGTGGGCG AGCCCCCATC AGACAGTACC 
AGCTTCACCG ACACCAGCGT GGAGCAGGGA AAGAAATATG CCTTCCGTGT GCGGGCTGTG 
ACCTCAGTGG GAGCCGGAGA TGCCCTGGAG TCAGAGGAGG TGTTGGTGGC TCCTGAGGCT 
CTCCCGGGGC CCCCTTCTGC CCCAGCCATC CTGTCAGCCT CCAGTCAGAG CATCACTCTC 
ACATGGGGGG CACCACAGGG CCCTGGCAGT AACCACATCC TGGGCTACCT GATCGAGAAG 
CACAAGAAGG GGAGCAACAC CTGGATGGCT GTGAATGAGC AGCCCGTGTC TGAAAGAAGG 
TACACTGTGG TGGGTCTGCG ACAGGGCTGC CAGTATGAGT TCCGGGTCAC GGCCGTGACT 
CTGTCGGGCC CTGGAGATCC TGGGCCTCCA TCGGATGCTG TCTTTGCTAG AGACCCTATG 
AGACCCCCAG GGCCTGTGCG GGATCTACAG GTTACAGACA CATCAAACAC TAGCATCACC 
GTGAGCTGGA TGGTGCCGGA TGCCCGGGAT GCTGATGAAG CCCAGGGATA TATTGTGGAG 
TTGTGTGGTT CAGACAGCCA TCAATGGAGC CCATGTCACG TGGGAACTGT GCCGGGCACC 
ACCTTCACAG CCAAGGGGCT TCGGCCGCAA GAAGGCTACT TTGTGCGGGT GACAGCAGTT 
AATGATGGGG GCCGGAGCCA GGCCACTTCC CTGGACACAT TGGTTCATGC CATGCCGGCC 
ACTGTCTGTC CCAGGTTCCT CATGGACTCC GGCACAAAGG ATACCCTGAT GGTCAGGGTT 
GGGGACAGTA TTCGCGTTCC TCTCTCCTTT GAAGCTGCCC CCATGCCTGA GGTGACCTGG 
CTGAAGGACG GCTTGCCCTT GCCCAAAAGA AGTGTGACCA CTGTGAAGGC TGGCCTCACG 
CAGCTTCTGA TCCCTGCAGC TAGCCTCTCG GACTGTGGCC GATACACCGT GATGCTGAGG 
AACCTACAGG GGAAGGAAGC TACCCACAGC TTCTTCATCA ATGTAGCAGC ATGCCCACAG 
GCACCAGGTT CTATCTACCT ACAGGAGAAT GTGCCTGGGA CAGTGACGGT CCAGTGGGAA 
CCCTCTCCGG ATGAGGCTCA GGGCATCCCC TTGCATTACA CAGTGCTGAT GCGGTCCTCA 
TCTCACCGAT CCTGGCACGA GGTGGCCGAC CATGTCCGTA CCAACCGCTT TACCCTCCTA 
GGGGTCCTCC CTGGCCATGA GTACCACTTT CGGGTGCTGG CCAAGAATGA GCTGGGGGTC 
AGCAAACCTT CAGACACCAG CCAACCCTGG TGTATCCCCA GGCAGAGAGA CAGGTTCACA 
GTGAAGTCTC CGACATACCA GGATCCTGAC CTGAGCCAGA AGCCCCGCTT CCTGGTGGGT 
CTGCGGGCTC ACCTTCTGCC CCAGGGCTGT GAGTGCCGCA TGACGTGTGC CGTGCAGGGC 
TCACCCCAGC CTCATGTCAC CTGGTTCAAG AATGACCAAA GCTTGGACAG AAACCCTGCG 
GTGTACAGTA CTGACTTGCT GGGTGTGTGC TCCCTCGTCA TCCCCAGCGT GTCCCTTAAG 
GACAGTGGTG AATACAAGGC TGTGGCCAAG AACCCACTGG GACAAGCTGT CAGCACAGCC 
ACCCTCATTG TCACAGAATA CGACTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_808310.2
CDS	1..8550
Translation	MNEMRTQVRIEFHALATQRKPSASPPTIEEDMGQGCTLEALVIPSRRAEQSTQHPDSSFMATVRGAKSVVVSQWQLLQAGSAGSAARPDGSGSESLAASSSWKPRRRLEAGGPCRSWLVFLVSREGWSDSNPGYRKQFICRPQEIGFHSEEHREAGTMAAKPPKKSSIPGVSIRQLVDEIPEGCSTPDFKQKPVTLALPEGKNAIFRAVVCGEPRPEVHWQSSKGDLSNSAKYQISSAPGREEHVLQINKLTGEDSDLYRCTALNAYGEATCSVRLTVIEVGFRKNRKRQKEPQEDLRKELMDFRKMLKKRAPPPTPEKKMESEQVWQLLVTADRKDYEKICMQYGIVDFRGMLRKLQEMKKEQEDRMAQYVSAIANLRHIKVTKEGVATFDLELDLKNLESKIYLYKDGEMIPYGVDNQTKHCLRRLGKRYHFQIQDLRPEDAGLYQVMVEDAVVFSTELESRTIPPRVVVPLAETRCEERGDAVFECTLSNPCPSATWHFQHRPLRLSDKYEVFVSPDGLTHRLVVKGASSSDMGLYSLNTGLHASSAWLVVEGGKDKGPQTTDTDHRLQTPEALASEAEDAGGISIKGGQSRERGSLKEISGAQLTAGPDRGAFGKHGYPLVVDEDIVNSTWGPGQDKKSFLEGGQLRVTLPGDSQSQREGDCGRSLPRRSHLQGEDTESDLGFLERGQQGPGRVDSENGRWETAGGQSAGSSHPRDRRLESRGEGQEHSEGHGSELDRYGQGQCHDPQLGAGAGQRVPWGSQFDAEGLQLKKEGTEMWGDCLDVTEGRGNLRKERGTAAGSSNRARLWEARGGSGAGSPCVPEFPRGGAHSSKAGMGPECWPGGDAGHGEAGVYWGAGGYPGQTSEGNDTQKSSLSGDRKLLGCTSPEAKAEGSFQSADGHGIVGRVYKPGPEGPEDLRSQKSGLQEIQGRNGQGSAGALGRTVEGSRSLQFPQSWTVGQREAGKPGGAEYEDIGPQDDTWSNLREMGGHCGSGVLASGGREGSVGGSQVAALMMSSQRVDARNHRLVTSPGLGVQGSGRTLGHMEGLRDPEAVGSEEEFWNGSGRSQGKGSRGGMGLGGPEWAESRNEEGWENSEGMASRNGATCRDGSRMQEGTGSGPDIGCKAPCGAPAETESGKWGTYSHDSGVPGGLWSGSKSQCSPAGKVSGREASLRNGSAGLHGMPSSEAQQRDVFQGHGQTGHIGEHHCARGLGSSETVGSVGGHGRKDSGTAGKVGEGYTEAEPGHSGGLSSWGHTGDYEDFRVLGAFREGDFGNGTGVPLSMGPRSLERGDKEGDGERISFLGARTSTVGTGNWDKARHPGAPSPHEAGSEGHWAQVSGNALGCRDGSGIPEPWSAGDKTAYGEESKGLGPERTGPDGEVAFRGSSSSLRGMGPASEASYKSGTWGPGAVGSGFKVDCRNDLGCSGSMGSESKAGYKNKIESHGVRELGDLTGYKNDLGPSELISSRNEARGLEHSGDILSWNKAGLRDGLGETGRMESKNGVGYRGSSVGPGEMGSERKMHLADGSGRLGGPGSLAAPKEHESVGLGSVYEGAAEIGSKYNREKVRGMGFRDATGPEVGSGVAGMLDITGGIAHGDSAMGTEKLGTPDRPYILSGGQGTKNSLGGCVSLGIPDALGAVGSVGKPGIREWKDDSGFQGSLRDRGTPSEEIRSVDQAGAIGTSRLLDSRGAVEDGSGSENDFDFNKSGLETTGRCRVADQQGVTPQGCGGSLLNGRRKGTSSGSLSGVGQEVDRSSTPGRETKVMSGPGSGRATSHTRAADWEDGVQCSFGASSSLPDTDAIFRETHAGQGAFKGRGCETGQGAAGECQGPRSLGSRGSEFVGGRAGFTGSSSVPGRRDSTIYGDAVTSKPQEPQNELYSPLGRRDFKSVSGGIQEPGFQRGTGQGEGKESFQESGSWEAELDEVQGSRASAKFENQGGKDPGWSGQKPGPCGSRHQVQSGTEVGSAKRATPQGAGGLEPWAGSEDRGSLREPWSEDRRQGPHRHLGSRRDTQEGRSDVCGQAQDATQSPRSRYQPGTGRSSSEARGSMDHFSQGLTDTEVQLGEAAIVSCTLASDLGPGTWFKDGVKLTAQDGVIFEQDGLTHRLILTHVEGTQAGKYTFVAGCQHSEASLTVQDPPTIAPDVTETLREPLVFKAGKPVTVKIPFQSRLPVQAAWRKDGNEVVGGDHKGIQLALADGYTWLCLPSVCRKDSGRYSVTLRSEGGCVQAEFTLQVIDKPQPPQGPLEVQDCHRAGVCLRWLPPRDNGGQVIQHYVVERRQAGRSTWLKVGEPPSDSTSFTDTSVEQGKKYAFRVRAVTSVGAGDALESEEVLVAPEALPGPPSAPAILSASSQSITLTWGAPQGPGSNHILGYLIEKHKKGSNTWMAVNEQPVSERRYTVVGLRQGCQYEFRVTAVTLSGPGDPGPPSDAVFARDPMRPPGPVRDLQVTDTSNTSITVSWMVPDARDADEAQGYIVELCGSDSHQWSPCHVGTVPGTTFTAKGLRPQEGYFVRVTAVNDGGRSQATSLDTLVHAMPATVCPRFLMDSGTKDTLMVRVGDSIRVPLSFEAAPMPEVTWLKDGLPLPKRSVTTVKAGLTQLLIPAASLSDCGRYTVMLRNLQGKEATHSFFINVAACPQAPGSIYLQENVPGTVTVQWEPSPDEAQGIPLHYTVLMRSSSHRSWHEVADHVRTNRFTLLGVLPGHEYHFRVLAKNELGVSKPSDTSQPWCIPRQRDRFTVKSPTYQDPDLSQKPRFLVGLRAHLLPQGCECRMTCAVQGSPQPHVTWFKNDQSLDRNPAVYSTDLLGVCSLVIPSVSLKDSGEYKAVAKNPLGQAVSTATLIVTEYDS

Target ORF information:

RefSeq Version	NM_177642.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus immunoglobulin-like and fibronectin type III domain containing 1 (Igfn1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177642.3

ORF Insert Sequence:

ATGAATGAGA TGCGAACACA AGTTAGAATT GAGTTTCATG CTTTGGCAAC TCAAAGGAAG 
CCTTCAGCAT CACCTCCTAC CATTGAGGAG GACATGGGCC AAGGTTGTAC TCTAGAAGCT 
CTGGTCATCC CTAGCAGAAG AGCTGAGCAA TCAACTCAGC ACCCAGACAG CTCATTCATG 
GCCACGGTCA GAGGTGCTAA ATCTGTAGTA GTCAGTCAAT GGCAGCTGCT ACAGGCCGGA 
TCCGCAGGCT CTGCCGCCAG ACCAGACGGC TCCGGCTCTG AGTCCCTGGC AGCCAGCAGC 
AGCTGGAAAC CAAGAAGGAG GCTGGAAGCT GGTGGGCCAT GCAGATCATG GCTGGTGTTT 
CTGGTGTCAC GGGAAGGATG GAGTGATAGC AACCCGGGCT ATCGTAAACA ATTCATCTGT 
CGCCCTCAGG AGATCGGTTT CCACTCAGAG GAGCACAGAG AAGCTGGGAC TATGGCTGCA 
AAGCCCCCTA AGAAGTCCTC CATCCCAGGA GTGAGCATCA GGCAGCTGGT GGACGAGATC 
CCAGAAGGCT GCAGCACACC AGATTTCAAG CAGAAGCCTG TTACCTTGGC TCTACCAGAG 
GGGAAAAACG CCATCTTTCG GGCGGTGGTC TGTGGGGAAC CCAGACCTGA AGTGCACTGG 
CAGAGCAGCA AAGGAGACCT CAGCAACTCC GCCAAGTACC AGATATCCTC TGCTCCCGGT 
AGAGAGGAGC ACGTGCTTCA GATCAACAAG CTGACTGGAG AGGATTCGGA TCTGTACCGC 
TGCACTGCAC TAAATGCCTA TGGAGAAGCC ACATGCTCAG TGAGGCTTAC GGTCATTGAA 
GTGGGTTTTC GGAAGAACCG AAAAAGGCAA AAGGAACCTC AGGAGGACCT CAGGAAGGAG 
CTGATGGACT TTCGGAAGAT GCTGAAAAAA CGGGCCCCAC CCCCAACCCC TGAAAAAAAG 
ATGGAGTCTG AGCAGGTGTG GCAGCTTTTG GTGACTGCAG ACCGTAAGGA CTACGAGAAG 
ATCTGTATGC AGTATGGTAT CGTCGACTTC CGGGGCATGC TGCGCAAGTT GCAGGAGATG 
AAAAAGGAAC AGGAGGACAG AATGGCCCAG TATGTCAGTG CCATTGCCAA CTTGAGACAC 
ATCAAGGTCA CCAAGGAAGG GGTCGCTACG TTTGACCTGG AGCTGGATCT CAAGAACCTC 
GAGAGCAAGA TTTACTTGTA CAAGGATGGT GAGATGATTC CCTACGGTGT TGATAACCAG 
ACCAAGCACT GTCTGAGACG GCTGGGGAAG CGCTACCACT TCCAGATCCA GGACCTGCGG 
CCTGAGGATG CTGGCCTTTA CCAGGTCATG GTGGAGGATG CTGTGGTCTT CTCTACAGAG 
CTAGAATCCA GAACCATCCC CCCCAGAGTG GTGGTCCCAT TGGCAGAGAC CCGCTGTGAG 
GAACGGGGTG ATGCAGTCTT TGAATGTACC CTCTCCAATC CCTGTCCCAG TGCGACCTGG 
CATTTCCAGC ACCGACCACT GCGGCTCAGT GACAAATATG AAGTATTTGT GTCCCCTGAT 
GGGCTAACCC ACCGACTGGT TGTGAAGGGG GCCAGTAGCT CAGACATGGG CCTCTACTCG 
CTGAACACTG GGCTCCATGC CTCCAGTGCC TGGCTGGTGG TTGAAGGTGG GAAGGATAAA 
GGCCCTCAGA CCACAGATAC TGACCACCGG CTGCAGACTC CAGAAGCTCT GGCCTCCGAA 
GCAGAAGACG CTGGAGGCAT CAGCATCAAG GGAGGGCAAT CCAGAGAGCG GGGTTCCCTC 
AAAGAGATTA GTGGGGCACA GCTCACAGCA GGCCCAGACA GGGGTGCCTT TGGCAAACAT 
GGCTATCCCT TGGTGGTGGA TGAAGACATA GTTAATTCTA CTTGGGGACC TGGGCAGGAC 
AAAAAGAGCT TTCTGGAAGG AGGACAACTC AGGGTCACTC TTCCAGGGGA TAGTCAGTCC 
CAAAGAGAGG GAGACTGTGG TAGAAGCCTC CCCAGAAGGT CTCATCTCCA GGGAGAGGAT 
ACAGAATCAG ATTTGGGCTT CCTGGAGAGA GGACAACAAG GGCCTGGTAG AGTTGACAGT 
GAGAATGGCA GATGGGAGAC AGCAGGAGGC CAGAGTGCTG GCTCCAGCCA TCCTCGGGAC 
AGAAGGCTTG AGAGCAGAGG AGAAGGACAG GAACACAGTG AGGGCCATGG CAGTGAACTG 
GACAGATATG GCCAGGGACA ATGCCATGAT CCTCAGCTGG GAGCAGGGGC AGGGCAGAGG 
GTGCCGTGGG GATCCCAGTT TGATGCTGAG GGGCTTCAGC TGAAAAAGGA GGGGACAGAA 
ATGTGGGGCG ATTGTCTGGA TGTGACGGAA GGTAGGGGTA ACCTGAGAAA GGAGAGGGGA 
ACAGCAGCAG GGTCAAGTAA CAGAGCCCGC CTCTGGGAAG CTCGAGGAGG CAGTGGGGCC 
GGAAGTCCTT GCGTCCCTGA GTTTCCTAGA GGAGGAGCTC ACAGCTCCAA GGCAGGCATG 
GGCCCTGAGT GCTGGCCAGG TGGAGATGCT GGCCATGGAG AAGCTGGGGT ATACTGGGGG 
GCAGGGGGAT ATCCAGGACA GACATCTGAG GGCAATGACA CCCAGAAATC ATCACTATCT 
GGAGACAGGA AACTTCTGGG GTGCACGAGT CCTGAGGCCA AAGCTGAGGG CTCTTTCCAG 
AGCGCAGATG GTCATGGGAT TGTGGGGAGG GTCTACAAAC CTGGCCCTGA AGGCCCAGAA 
GATCTTAGGA GTCAGAAAAG TGGCCTACAA GAGATTCAGG GAAGGAATGG TCAAGGCTCA 
GCTGGAGCCT TGGGCAGGAC GGTGGAAGGT TCCAGAAGCC TCCAATTTCC TCAGAGCTGG 
ACAGTGGGAC AGAGGGAAGC AGGGAAGCCA GGTGGAGCAG AGTATGAAGA CATAGGTCCT 
CAGGATGACA CGTGGTCCAA TCTCAGAGAG ATGGGAGGCC ACTGTGGGTC TGGAGTGCTG 
GCGTCTGGTG GGAGGGAAGG GAGCGTGGGC GGTTCCCAAG TAGCTGCCCT GATGATGTCT 
AGTCAGAGGG TAGATGCCAG AAACCACAGG CTAGTTACAT CCCCAGGCCT GGGTGTCCAG 
GGTTCTGGCA GAACACTGGG ACACATGGAA GGATTAAGAG ACCCAGAGGC AGTGGGATCC 
GAAGAAGAGT TCTGGAATGG GTCAGGAAGG TCCCAAGGAA AAGGGTCCAG AGGTGGGATG 
GGCTTAGGAG GTCCAGAGTG GGCAGAGTCT AGGAATGAGG AGGGATGGGA GAATTCTGAA 
GGGATGGCTT CTAGGAATGG AGCCACTTGT CGTGATGGAT CCAGGATGCA GGAAGGAACA 
GGATCTGGCC CTGATATTGG TTGCAAGGCC CCCTGTGGGG CACCTGCGGA GACAGAGTCT 
GGGAAGTGGG GTACTTACAG TCATGACTCA GGGGTGCCTG GGGGATTGTG GTCTGGAAGC 
AAAAGTCAGT GTAGTCCTGC AGGAAAGGTA TCTGGAAGAG AGGCCAGCCT CAGAAATGGC 
TCAGCTGGCC TTCATGGAAT GCCTTCTAGT GAAGCTCAGC AGAGGGATGT ATTTCAAGGC 
CACGGTCAGA CAGGCCACAT TGGAGAGCAC CACTGTGCCC GTGGCTTAGG GAGTTCTGAG 
ACAGTGGGAT CTGTGGGTGG ACATGGTAGG AAAGACTCTG GAACAGCGGG CAAGGTTGGA 
GAGGGATACA CAGAAGCAGA ACCAGGGCAC TCTGGAGGGT TGAGTTCTTG GGGTCACACT 
GGAGATTATG AGGACTTCCG AGTCCTGGGG GCCTTTAGGG AAGGAGACTT TGGCAATGGC 
ACTGGGGTTC CATTATCCAT GGGACCAAGG TCTCTGGAGA GAGGGGACAA GGAAGGAGAT 
GGAGAAAGGA TCAGTTTTCT TGGTGCCAGG ACCTCTACAG TTGGAACTGG GAATTGGGAC 
AAAGCCAGGC ACCCTGGGGC ACCATCTCCT CATGAGGCCG GTTCAGAGGG CCACTGGGCT 
CAAGTGTCTG GCAATGCATT AGGTTGTAGA GATGGGTCTG GAATTCCAGA GCCTTGGAGT 
GCTGGCGACA AAACAGCTTA TGGAGAAGAA TCAAAGGGTC TTGGGCCTGA AAGAACAGGA 
CCAGATGGTG AGGTGGCATT TAGAGGTAGC TCTTCGAGCC TCAGAGGAAT GGGACCAGCA 
AGTGAGGCAA GTTATAAAAG TGGAACTTGG GGCCCTGGGG CTGTGGGTTC AGGGTTTAAG 
GTAGATTGTA GGAATGATCT TGGGTGTTCT GGAAGTATGG GGTCAGAGAG TAAAGCAGGT 
TATAAGAACA AAATTGAGAG TCATGGGGTC AGGGAGTTAG GGGACCTGAC AGGCTATAAA 
AATGATTTAG GACCTTCTGA ACTTATTTCA TCAAGGAATG AGGCACGTGG TTTAGAACAT 
TCTGGAGACA TTTTGTCATG GAATAAGGCA GGTTTAAGGG ATGGCTTGGG AGAAACAGGA 
AGAATGGAGT CCAAGAATGG GGTTGGTTAT AGGGGAAGCT CAGTGGGTCC TGGGGAAATG 
GGGTCAGAGA GGAAAATGCA CCTTGCAGAT GGTTCAGGGA GGCTAGGAGG ACCAGGGTCA 
CTGGCTGCGC CCAAAGAACA TGAATCTGTG GGTCTTGGGT CAGTTTATGA GGGAGCAGCA 
GAGATTGGCT CCAAATATAA TAGGGAGAAA GTCAGAGGAA TGGGGTTTAG AGATGCAACA 
GGGCCTGAGG TGGGCTCTGG GGTAGCTGGA ATGCTGGATA TTACAGGTGG CATAGCACAC 
GGGGACAGTG CTATGGGCAC AGAGAAGCTG GGGACACCAG ATAGGCCTTA TATCTTGTCA 
GGTGGGCAGG GTACCAAGAA CAGTCTTGGG GGCTGTGTGT CCTTGGGAAT CCCTGACGCC 
TTGGGGGCTG TTGGCTCTGT GGGGAAACCA GGTATCAGAG AATGGAAAGA TGATTCTGGG 
TTCCAAGGGT CTCTCAGGGA CAGAGGGACT CCGAGTGAGG AGATCAGATC TGTAGACCAA 
GCAGGAGCTA TAGGGACATC TCGGCTTCTT GATAGTAGGG GGGCAGTGGA GGATGGGAGT 
GGGTCAGAAA ATGACTTTGA CTTTAATAAA TCAGGCTTGG AGACAACAGG AAGATGTAGA 
GTTGCTGACC AGCAGGGAGT GACACCACAG GGATGTGGAG GCTCATTGCT CAATGGCAGG 
AGGAAGGGAA CAAGTTCTGG AAGCTTGTCA GGGGTAGGGC AGGAGGTAGA CAGAAGTTCT 
ACACCTGGGA GGGAGACCAA GGTAATGTCA GGACCTGGCA GTGGGCGAGC CACAAGCCAC 
ACTCGGGCTG CAGACTGGGA AGACGGGGTT CAATGCAGCT TCGGGGCTTC TAGCTCACTG 
CCTGATACTG ATGCCATTTT CAGAGAAACC CATGCGGGAC AAGGAGCCTT CAAGGGCAGG 
GGCTGTGAAA CCGGCCAAGG GGCTGCTGGA GAGTGTCAAG GACCACGCTC CTTGGGGAGC 
AGAGGCTCAG AGTTTGTAGG GGGCAGGGCT GGATTTACAG GGAGCTCAAG TGTTCCAGGC 
AGAAGGGATT CCACTATCTA CGGAGATGCT GTCACATCAA AGCCACAGGA ACCCCAGAAT 
GAATTATATA GTCCTTTAGG CAGAAGGGAC TTCAAAAGTG TATCGGGAGG AATCCAGGAG 
CCAGGTTTTC AACGAGGAAC AGGACAGGGA GAAGGAAAAG AATCCTTCCA GGAGTCAGGG 
TCCTGGGAGG CTGAGCTTGA CGAGGTCCAA GGATCCAGGG CCTCAGCAAA GTTTGAAAAC 
CAGGGAGGGA AAGACCCTGG CTGGTCAGGC CAGAAGCCTG GTCCCTGTGG AAGCAGGCAT 
CAGGTGCAGT CTGGGACTGA AGTTGGATCA GCAAAGAGAG CAACCCCACA GGGGGCCGGA 
GGGCTGGAGC CTTGGGCTGG CAGTGAGGAT AGAGGGTCTC TGAGAGAACC TTGGAGTGAA 
GACAGGAGGC AAGGCCCCCA CAGGCACCTT GGCAGCAGGA GAGATACCCA AGAGGGCAGG 
TCAGATGTCT GTGGCCAGGC ACAAGATGCT ACCCAGAGCC CCAGATCCAG ATACCAGCCT 
GGCACTGGCC GCTCTTCTTC AGAGGCCCGA GGCTCCATGG ACCACTTCTC TCAGGGTCTG 
ACTGATACAG AGGTGCAACT GGGGGAAGCC GCCATAGTCT CCTGTACTCT TGCCAGTGAC 
CTGGGACCTG GCACGTGGTT CAAGGATGGA GTCAAGCTCA CTGCCCAAGA TGGAGTCATC 
TTTGAGCAAG ATGGGCTCAC GCACAGACTG ATTCTCACCC ACGTGGAGGG GACCCAGGCT 
GGGAAATACA CTTTTGTAGC CGGCTGCCAG CACAGCGAGG CCAGCCTGAC CGTGCAGGAT 
CCCCCCACCA TTGCTCCAGA TGTGACAGAG ACACTGAGAG AGCCACTGGT GTTCAAGGCT 
GGGAAGCCAG TCACCGTGAA AATCCCTTTC CAGAGCCGCC TCCCTGTTCA GGCTGCCTGG 
AGGAAAGATG GGAACGAGGT GGTGGGCGGC GACCACAAGG GCATCCAACT GGCTCTGGCA 
GATGGCTACA CATGGCTGTG CCTCCCCAGC GTGTGCAGGA AGGACAGTGG CCGGTACAGC 
GTGACGCTGA GGAGCGAGGG AGGCTGTGTG CAGGCTGAGT TCACCCTGCA AGTTATAGAC 
AAGCCTCAGC CCCCACAAGG ACCCCTGGAG GTTCAGGATT GCCACAGAGC AGGTGTCTGC 
CTCCGCTGGC TACCCCCGAG GGACAACGGG GGCCAGGTCA TACAACACTA TGTGGTGGAG 
AGGCGACAGG CTGGAAGGAG CACTTGGTTG AAGGTGGGCG AGCCCCCATC AGACAGTACC 
AGCTTCACCG ACACCAGCGT GGAGCAGGGA AAGAAATATG CCTTCCGTGT GCGGGCTGTG 
ACCTCAGTGG GAGCCGGAGA TGCCCTGGAG TCAGAGGAGG TGTTGGTGGC TCCTGAGGCT 
CTCCCGGGGC CCCCTTCTGC CCCAGCCATC CTGTCAGCCT CCAGTCAGAG CATCACTCTC 
ACATGGGGGG CACCACAGGG CCCTGGCAGT AACCACATCC TGGGCTACCT GATCGAGAAG 
CACAAGAAGG GGAGCAACAC CTGGATGGCT GTGAATGAGC AGCCCGTGTC TGAAAGAAGG 
TACACTGTGG TGGGTCTGCG ACAGGGCTGC CAGTATGAGT TCCGGGTCAC GGCCGTGACT 
CTGTCGGGCC CTGGAGATCC TGGGCCTCCA TCGGATGCTG TCTTTGCTAG AGACCCTATG 
AGACCCCCAG GGCCTGTGCG GGATCTACAG GTTACAGACA CATCAAACAC TAGCATCACC 
GTGAGCTGGA TGGTGCCGGA TGCCCGGGAT GCTGATGAAG CCCAGGGATA TATTGTGGAG 
TTGTGTGGTT CAGACAGCCA TCAATGGAGC CCATGTCACG TGGGAACTGT GCCGGGCACC 
ACCTTCACAG CCAAGGGGCT TCGGCCGCAA GAAGGCTACT TTGTGCGGGT GACAGCAGTT 
AATGATGGGG GCCGGAGCCA GGCCACTTCC CTGGACACAT TGGTTCATGC CATGCCGGCC 
ACTGTCTGTC CCAGGTTCCT CATGGACTCC GGCACAAAGG ATACCCTGAT GGTCAGGGTT 
GGGGACAGTA TTCGCGTTCC TCTCTCCTTT GAAGCTGCCC CCATGCCTGA GGTGACCTGG 
CTGAAGGACG GCTTGCCCTT GCCCAAAAGA AGTGTGACCA CTGTGAAGGC TGGCCTCACG 
CAGCTTCTGA TCCCTGCAGC TAGCCTCTCG GACTGTGGCC GATACACCGT GATGCTGAGG 
AACCTACAGG GGAAGGAAGC TACCCACAGC TTCTTCATCA ATGTAGCAGC ATGCCCACAG 
GCACCAGGTT CTATCTACCT ACAGGAGAAT GTGCCTGGGA CAGTGACGGT CCAGTGGGAA 
CCCTCTCCGG ATGAGGCTCA GGGCATCCCC TTGCATTACA CAGTGCTGAT GCGGTCCTCA 
TCTCACCGAT CCTGGCACGA GGTGGCCGAC CATGTCCGTA CCAACCGCTT TACCCTCCTA 
GGGGTCCTCC CTGGCCATGA GTACCACTTT CGGGTGCTGG CCAAGAATGA GCTGGGGGTC 
AGCAAACCTT CAGACACCAG CCAACCCTGG TGTATCCCCA GGCAGAGAGA CAGGTTCACA 
GTGAAGTCTC CGACATACCA GGATCCTGAC CTGAGCCAGA AGCCCCGCTT CCTGGTGGGT 
CTGCGGGCTC ACCTTCTGCC CCAGGGCTGT GAGTGCCGCA TGACGTGTGC CGTGCAGGGC 
TCACCCCAGC CTCATGTCAC CTGGTTCAAG AATGACCAAA GCTTGGACAG AAACCCTGCG 
GTGTACAGTA CTGACTTGCT GGGTGTGTGC TCCCTCGTCA TCCCCAGCGT GTCCCTTAAG 
GACAGTGGTG AATACAAGGC TGTGGCCAAG AACCCACTGG GACAAGCTGT CAGCACAGCC 
ACCCTCATTG TCACAGAATA CGACTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Igfn1 ( XM_017319938.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175427.1 Mus musculus immunoglobulin-like and fibronectin type III domain containing 1 (Igfn1), transcript variant X2, mRNA.

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CloneID	OMu77088
Clone ID Related Accession (Same CDS sequence)	XM_017319938.1 , XM_017319940.1 , XM_017319937.1
Accession Version	XM_017319938.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8079bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	immunoglobulin-like and fibronectin type III domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCAA AGCCCCCTAA GAAGTCCTCC ATCCCAGGAG TGAGCATCAG GCAGCTGGTG 
GACGAGATCC CAGAAGGCTG CAGCACACCA GATTTCAAGC AGAAGCCTGT TACCTTGGCT 
CTACCAGAGG GGAAAAACGC CATCTTTCGG GCGGTGGTCT GTGGGGAACC CAGACCTGAA 
GTGCACTGGC AGAGCAGCAA AGGAGACCTC AGCAACTCCG CCAAGTACCA GATATCCTCT 
GCTCCCGGTA GAGAGGAGCA CGTGCTTCAG ATCAACAAGC TGACTGGAGA GGATTCGGAT 
CTGTACCGCT GCACTGCACT AAATGCCTAT GGAGAAGCCA CATGCTCAGT GAGGCTTACG 
GTCATTGAAG TGGGTTTTCG GAAGAACCGA AAAAGGCAAA AGGAACCTCA GGAGGACCTC 
AGGAAGGAGC TGATGGACTT TCGGAAGATG CTGAAAAAAC GGGCCCCACC CCCAACCCCT 
GAAAAAAAGA TGGAGTCTGA GCAGGTGTGG CAGCTTTTGG TGACTGCAGA CCGTAAGGAC 
TACGAGAAGA TCTGTATGCA GTATGGTATC GTCGACTTCC GGGGCATGCT GCGCAAGTTG 
CAGGAGATGA AAAAGGAACA GGAGGACAGA ATGGCCCAGT ATGTCAGTGC CATTGCCAAC 
TTGAGACACA TCAAGGTCAC CAAGGAAGGG GTCGCTACGT TTGACCTGGA GCTGGATCTC 
AAGAACCTCG AGAGCAAGAT TTACTTGTAC AAGGATGGTG AGATGATTCC CTACGGTGTT 
GATAACCAGA CCAAGCACTG TCTGAGACGG CTGGGGAAGC GCTACCACTT CCAGATCCAG 
GACCTGCGGC CTGAGGATGC TGGCCTTTAC CAGGTCATGG TGGAGGATGC TGTGGTCTTC 
TCTACAGAGC TAGAATCCAG AACCATCCCC CCCAGAGTGG TGGTCCCATT GGCAGAGACC 
CGCTGTGAGG AACGGGGTGA TGCAGTCTTT GAATGTACCC TCTCCAATCC CTGTCCCAGT 
GCGACCTGGC ATTTCCAGCA CCGACCACTG CGGCTCAGTG ACAAATATGA AGTATTTGTG 
TCCCCTGATG GGCTAACCCA CCGACTGGTT GTGAAGGGGG CCAGTAGCTC AGACATGGGC 
CTCTACTCGC TGAACACTGG GCTCCATGCC TCCAGTGCCT GGCTGGTGGT TGAAGGTGGG 
AAGGATAAAG GCCCTCAGAC CACAGATACT GACCACCGGC TGCAGACTCC AGAAGCTCTG 
GCCTCCGAAG CAGAAGACGC TGGAGGCATC AGCATCAAGG GAGGGCAATC CAGAGAGCGG 
GGTTCCCTCA AAGAGATTAG TGGGGCACAG CTCACAGCAG GCCCAGACAG GGGTGCCTTT 
GGCAAACATG GCTATCCCTT GGTGGTGGAT GAAGACATAG TTAATTCTAC TTGGGGACCT 
GGGCAGGACA AAAAGAGCTT TCTGGAAGGA GGACAACTCA GGGTCACTCT TCCAGGGGAT 
AGTCAGTCCC AAAGAGAGGG AGACTGTGGT AGAAGCCTCC CCAGAAGGTC TCATCTCCAG 
GGAGAGGATA CAGAATCAGA TTTGGGCTTC CTGGAGAGAG GACAACAAGG GCCTGGTAGA 
GTTGACAGTG AGAATGGCAG ATGGGAGACA GCAGGAGGCC AGAGTGCTGG CTCCAGCCAT 
CCTCGGGACA GAAGGCTTGA GAGCAGAGGA GAAGGACAGG AACACAGTGA GGGCCATGGC 
AGTGAACTGG ACAGATATGG CCAGGGACAA TGCCATGATC CTCAGCTGGG AGCAGGGGCA 
GGGCAGAGGG TGCCGTGGGG ATCCCAGTTT GATGCTGAGG GGCTTCAGCT GAAAAAGGAG 
GGGACAGAAA TGTGGGGCGA TTGTCTGGAT GTGACGGAAG GTAGGGGTAA CCTGAGAAAG 
GAGAGGGGAA CAGCAGCAGG GTCAAGTAAC AGAGCCCGCC TCTGGGAAGC TCGAGGAGGC 
AGTGGGGCCG GAAGTCCTTG CGTCCCTGAG TTTCCTAGAG GAGGAGCTCA CAGCTCCAAG 
GCAGGCATGG GCCCTGAGTG CTGGCCAGGT GGAGATGCTG GCCATGGAGA AGCTGGGGTA 
TACTGGGGGG CAGGGGGATA TCCAGGACAG ACATCTGAGG GCAATGACAC CCAGAAATCA 
TCACTATCTG GAGACAGGAA ACTTCTGGGG TGCACGAGTC CTGAGGCCAA AGCTGAGGGC 
TCTTTCCAGA GCGCAGATGG TCATGGGATT GTGGGGAGGG TCTACAAACC TGGCCCTGAA 
GGCCCAGAAG ATCTTAGGAG TCAGAAAAGT GGCCTACAAG AGATTCAGGG AAGGAATGGT 
CAAGGCTCAG CTGGAGCCTT GGGCAGGACG GTGGAAGGTT CCAGAAGCCT CCAATTTCCT 
CAGAGCTGGA CAGTGGGACA GAGGGAAGCA GGGAAGCCAG GTGGAGCAGA GTATGAAGAC 
ATAGGTCCTC AGGATGACAC GTGGTCCAAT CTCAGAGAGA TGGGAGGCCA CTGTGGGTCT 
GGAGTGCTGG CGTCTGGTGG GAGGGAAGGG AGCGTGGGCG GTTCCCAAGT AGCTGCCCTG 
ATGATGTCTA GTCAGAGGGT AGATGCCAGA AACCACAGGC TAGTTACATC CCCAGGCCTG 
GGTGTCCAGG GTTCTGGCAG AACACTGGGA CACATGGAAG GATTAAGAGA CCCAGAGGCA 
GTGGGATCCG AAGAAGAGTT CTGGAATGGG TCAGGAAGGT CCCAAGGAAA AGGGTCCAGA 
GGTGGGATGG GCTTAGGAGG TCCAGAGTGG GCAGAGTCTA GGAATGAGGA GGGATGGGAG 
AATTCTGAAG GGATGGCTTC TAGGAATGGA GCCACTTGTC GTGATGGATC CAGGATGCAG 
GAAGGAACAG GATCTGGCCC TGATATTGGT TGCAAGGCCC CCTGTGGGGC ACCTGCGGAG 
ACAGAGTCTG GGAAGTGGGG TACTTACAGT CATGACTCAG GGGTGCCTGG GGGATTGTGG 
TCTGGAAGCA AAAGTCAGTG TAGTCCTGCA GGAAAGGTAT CTGGAAGAGA GGCCAGCCTC 
AGAAATGGCT CAGCTGGCCT TCATGGAATG CCTTCTAGTG AAGCTCAGCA GAGGGATGTA 
TTTCAAGGCC ACGGTCAGAC AGGCCACATT GGAGAGCACC ACTGTGCCCG TGGCTTAGGG 
AGTTCTGAGA CAGTGGGATC TGTGGGTGGA CATGGTAGGA AAGACTCTGG AACAGCGGGC 
AAGGTTGGAG AGGGATACAC AGAAGCAGAA CCAGGGCACT CTGGAGGGTT GAGTTCTTGG 
GGTCACACTG GAGATTATGA GGACTTCCGA GTCCTGGGGG CCTTTAGGGA AGGAGACTTT 
GGCAATGGCA CTGGGGTTCC ATTATCCATG GGACCAAGGT CTCTGGAGAG AGGGGACAAG 
GAAGGAGATG GAGAAAGGAT CAGTTTTCTT GGTGCCAGGA CCTCTACAGT TGGAACTGGG 
AATTGGGACA AAGCCAGGCA CCCTGGGGCA CCATCTCCTC ATGAGGCCGG TTCAGAGGGC 
CACTGGGCTC AAGTGTCTGG CAATGCATTA GGTTGTAGAG ATGGGTCTGG AATTCCAGAG 
CCTTGGAGTG CTGGCGACAA AACAGCTTAT GGAGAAGAAT CAAAGGGTCT TGGGCCTGAA 
AGAACAGGAC CAGATGGTGA GGTGGCATTT AGAGGTAGCT CTTCGAGCCT CAGAGGAATG 
GGACCAGCAA GTGAGGCAAG TTATAAAAGT GGAACTTGGG GCCCTGGGGC TGTGGGTTCA 
GGGTTTAAGG TAGATTGTAG GAATGATCTT GGGTGTTCTG GAAGTATGGG GTCAGAGAGT 
AAAGCAGGTT ATAAGAACAA AATTGAGAGT CATGGGGTCA GGGAGTTAGG GGACCTGACA 
GGCTATAAAA ATGATTTAGG ACCTTCTGAA CTTATTTCAT CAAGGAATGA GGCACGTGGT 
TTAGAACATT CTGGAGACAT TTTGTCATGG AATAAGGCAG GTTTAAGGGA TGGCTTGGGA 
GAAACAGGAA GAATGGAGTC CAAGAATGGG GTTGGTTATA GGGGAAGCTC AGTGGGTCCT 
GGGGAAATGG GGTCAGAGAG GAAAATGCAC CTTGCAGATG GTTCAGGGAG GCTAGGAGGA 
CCAGGGTCAC TGGCTGCGCC CAAAGAACAT GAATCTGTGG GTCTTGGGTC AGTTTATGAG 
GGAGCAGCAG AGATTGGCTC CAAATATAAT AGGGAGAAAG TCAGAGGAAT GGGGTTTAGA 
GATGCAACAG GGCCTGAGGT GGGCTCTGGG GTAGCTGGAA TGCTGGATAT TACAGGTGGC 
ATAGCACACG GGGACAGTGC TATGGGCACA GAGAAGCTGG GGACACCAGA TAGGCCTTAT 
ATCTTGTCAG GTGGGCAGGG TACCAAGAAC AGTCTTGGGG GCTGTGTGTC CTTGGGAATC 
CCTGACGCCT TGGGGGCTGT TGGCTCTGTG GGGAAACCAG GTATCAGAGA ATGGAAAGAT 
GATTCTGGGT TCCAAGGGTC TCTCAGGGAC AGAGGGACTC CGAGTGAGGA GATCAGATCT 
GTAGACCAAG CAGGAGCTAT AGGGACATCT CGGCTTCTTG ATAGTAGGGG GGCAGTGGAG 
GATGGGAGTG GGTCAGAAAA TGACTTTGAC TTTAATAAAT CAGGCTTGGA GACAACAGGA 
AGATGTAGAG TTGCTGACCA GCAGGGAGTG ACACCACAGG GATGTGGAGG CTCATTGCTC 
AATGGCAGGA GGAAGGGAAC AAGTTCTGGA AGCTTGTCAG GGGTAGGGCA GGAGGTAGAC 
AGAAGTTCTA CACCTGGGAG GGAGACCAAG GTAATGTCAG GACCTGGCAG TGGGCGAGCC 
ACAAGCCACA CTCGGGCTGC AGACTGGGAA GACGGGGTTC AATGCAGCTT CGGGGCTTCT 
AGCTCACTGC CTGATACTGA TGCCATTTTC AGAGAAACCC ATGCGGGACA AGGAGCCTTC 
AAGGGCAGGG GCTGTGAAAC CGGCCAAGGG GCTGCTGGAG AGTGTCAAGG ACCACGCTCC 
TTGGGGAGCA GAGGCTCAGA GTTTGTAGGG GGCAGGGCTG GATTTACAGG GAGCTCAAGT 
GTTCCAGGCA GAAGGGATTC CACTATCTAC GGAGATGCTG TCACATCAAA GCCACAGGAA 
CCCCAGAATG AATTATATAG TCCTTTAGGC AGAAGGGACT TCAAAAGTGT ATCGGGAGGA 
ATCCAGGAGC CAGGTTTTCA ACGAGGAACA GGACAGGGAG AAGGAAAAGA ATCCTTCCAG 
GAGTCAGGGT CCTGGGAGGC TGAGCTTGAC GAGGTCCAAG GATCCAGGGC CTCAGCAAAG 
TTTGAAAACC AGGGAGGGAA AGACCCTGGC TGGTCAGGCC AGAAGCCTGG TCCCTGTGGA 
AGCAGGCATC AGGTGCAGTC TGGGACTGAA GTTGGATCAG CAAAGAGAGC AACCCCACAG 
GGGGCCGGAG GGCTGGAGCC TTGGGCTGGC AGTGAGGATA GAGGGTCTCT GAGAGAACCT 
TGGAGTGAAG ACAGGAGGCA AGGCCCCCAC AGGCACCTTG GCAGCAGGAG AGATACCCAA 
GAGGGCAGGT CAGATGTCTG TGGCCAGGCA CAAGATGCTA CCCAGAGCCC CAGATCCAGA 
TACCAGCCTG GCACTGGCCG CTCTTCTTCA GAGGCCCGAG GCTCCATGGA CCACTTCTCT 
CAGGGTCTGA CTGATACAGA GGTGCAACTG GGGGAAGCCG CCATAGTCTC CTGTACTCTT 
GCCAGTGACC TGGGACCTGG CACGTGGTTC AAGGATGGAG TCAAGCTCAC TGCCCAAGAT 
GGAGTCATCT TTGAGCAAGA TGGGCTCACG CACAGACTGA TTCTCACCCA CGTGGAGGGG 
ACCCAGGCTG GGAAATACAC TTTTGTAGCC GGCTGCCAGC ACAGCGAGGC CAGCCTGACC 
GTGCAGGATC CCCCCACCAT TGCTCCAGAT GTGACAGAGA CACTGAGAGA GCCACTGGTG 
TTCAAGGCTG GGAAGCCAGT CACCGTGAAA ATCCCTTTCC AGAGCCGCCT CCCTGTTCAG 
GCTGCCTGGA GGAAAGATGG GAACGAGGTG GTGGGCGGCG ACCACAAGGG CATCCAACTG 
GCTCTGGCAG ATGGCTACAC ATGGCTGTGC CTCCCCAGCG TGTGCAGGAA GGACAGTGGC 
CGGTACAGCG TGACGCTGAG GAGCGAGGGA GGCTGTGTGC AGGCTGAGTT CACCCTGCAA 
GTTATAGACA AGCCTCAGCC CCCACAAGGA CCCCTGGAGG TTCAGGATTG CCACAGAGCA 
GGTGTCTGCC TCCGCTGGCT ACCCCCGAGG GACAACGGGG GCCAGGTCAT ACAACACTAT 
GTGGTGGAGA GGCGACAGGC TGGAAGGAGC ACTTGGTTGA AGGTGGGCGA GCCCCCATCA 
GACAGTACCA GCTTCACCGA CACCAGCGTG GAGCAGGGAA AGAAATATGC CTTCCGTGTG 
CGGGCTGTGA CCTCAGTGGG AGCCGGAGAT GCCCTGGAGT CAGAGGAGGT GTTGGTGGCT 
CCTGAGGCTC TCCCGGGGCC CCCTTCTGCC CCAGCCATCC TGTCAGCCTC CAGTCAGAGC 
ATCACTCTCA CATGGGGGGC ACCACAGGGC CCTGGCAGTA ACCACATCCT GGGCTACCTG 
ATCGAGAAGC ACAAGAAGGG GAGCAACACC TGGATGGCTG TGAATGAGCA GCCCGTGTCT 
GAAAGAAGGT ACACTGTGGT GGGTCTGCGA CAGGGCTGCC AGTATGAGTT CCGGGTCACG 
GCCGTGACTC TGTCGGGCCC TGGAGATCCT GGGCCTCCAT CGGATGCTGT CTTTGCTAGA 
GACCCTATGA GACCCCCAGG GCCTGTGCGG GATCTACAGG TTACAGACAC ATCAAACACT 
AGCATCACCG TGAGCTGGAT GGTGCCGGAT GCCCGGGATG CTGATGAAGC CCAGGGATAT 
ATTGTGGAGT TGTGTGGTTC AGACAGCCAT CAATGGAGCC CATGTCACGT GGGAACTGTG 
CCGGGCACCA CCTTCACAGC CAAGGGGCTT CGGCCGCAAG AAGGCTACTT TGTGCGGGTG 
ACAGCAGTTA ATGATGGGGG CCGGAGCCAG GCCACTTCCC TGGACACATT GGTTCATGCC 
ATGCCGGCCA CTGTCTGTCC CAGGTTCCTC ATGGACTCCG GCACAAAGGA TACCCTGATG 
GTCAGGGTTG GGGACAGTAT TCGCGTTCCT CTCTCCTTTG AAGCTGCCCC CATGCCTGAG 
GTGACCTGGC TGAAGGACGG CTTGCCCTTG CCCAAAAGAA GTGTGACCAC TGTGAAGGCT 
GGCCTCACGC AGCTTCTGAT CCCTGCAGCT AGCCTCTCGG ACTGTGGCCG ATACACCGTG 
ATGCTGAGGA ACCTACAGGG GAAGGAAGCT ACCCACAGCT TCTTCATCAA TGTAGCAGCA 
TGCCCACAGG CACCAGGTTC TATCTACCTA CAGGAGAATG TGCCTGGGAC AGTGACGGTC 
CAGTGGGAAC CCTCTCCGGA TGAGGCTCAG GGCATCCCCT TGCATTACAC AGTGCTGATG 
CGGTCCTCAT CTCACCGATC CTGGCACGAG GTGGCCGACC ATGTCCGTAC CAACCGCTTT 
ACCCTCCTAG GGGTCCTCCC TGGCCATGAG TACCACTTTC GGGTGCTGGC CAAGAATGAG 
CTGGGGGTCA GCAAACCTTC AGACACCAGC CAACCCTGGT GTATCCCCAG GCAGAGAGAC 
AGGTTCACAG TGAAGTCTCC GACATACCAG GATCCTGACC TGAGCCAGAA GCCCCGCTTC 
CTGGTGGGTC TGCGGGCTCA CCTTCTGCCC CAGGGCTGTG AGTGCCGCAT GACGTGTGCC 
GTGCAGGGCT CACCCCAGCC TCATGTCACC TGGTTCAAGA ATGACCAAAG CTTGGACAGA 
AACCCTGCGG TGTACAGTAC TGACTTGCTG GGTGTGTGCT CCCTCGTCAT CCCCAGCGTG 
TCCCTTAAGG ACAGTGGTGA ATACAAGGCT GTGGCCAAGA ACCCACTGGG ACAAGCTGTC 
AGCACAGCCA CCCTCATTGT CACAGAATAC GACTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175427.1
CDS	462..8540
Translation	MAAKPPKKSSIPGVSIRQLVDEIPEGCSTPDFKQKPVTLALPEGKNAIFRAVVCGEPRPEVHWQSSKGDLSNSAKYQISSAPGREEHVLQINKLTGEDSDLYRCTALNAYGEATCSVRLTVIEVGFRKNRKRQKEPQEDLRKELMDFRKMLKKRAPPPTPEKKMESEQVWQLLVTADRKDYEKICMQYGIVDFRGMLRKLQEMKKEQEDRMAQYVSAIANLRHIKVTKEGVATFDLELDLKNLESKIYLYKDGEMIPYGVDNQTKHCLRRLGKRYHFQIQDLRPEDAGLYQVMVEDAVVFSTELESRTIPPRVVVPLAETRCEERGDAVFECTLSNPCPSATWHFQHRPLRLSDKYEVFVSPDGLTHRLVVKGASSSDMGLYSLNTGLHASSAWLVVEGGKDKGPQTTDTDHRLQTPEALASEAEDAGGISIKGGQSRERGSLKEISGAQLTAGPDRGAFGKHGYPLVVDEDIVNSTWGPGQDKKSFLEGGQLRVTLPGDSQSQREGDCGRSLPRRSHLQGEDTESDLGFLERGQQGPGRVDSENGRWETAGGQSAGSSHPRDRRLESRGEGQEHSEGHGSELDRYGQGQCHDPQLGAGAGQRVPWGSQFDAEGLQLKKEGTEMWGDCLDVTEGRGNLRKERGTAAGSSNRARLWEARGGSGAGSPCVPEFPRGGAHSSKAGMGPECWPGGDAGHGEAGVYWGAGGYPGQTSEGNDTQKSSLSGDRKLLGCTSPEAKAEGSFQSADGHGIVGRVYKPGPEGPEDLRSQKSGLQEIQGRNGQGSAGALGRTVEGSRSLQFPQSWTVGQREAGKPGGAEYEDIGPQDDTWSNLREMGGHCGSGVLASGGREGSVGGSQVAALMMSSQRVDARNHRLVTSPGLGVQGSGRTLGHMEGLRDPEAVGSEEEFWNGSGRSQGKGSRGGMGLGGPEWAESRNEEGWENSEGMASRNGATCRDGSRMQEGTGSGPDIGCKAPCGAPAETESGKWGTYSHDSGVPGGLWSGSKSQCSPAGKVSGREASLRNGSAGLHGMPSSEAQQRDVFQGHGQTGHIGEHHCARGLGSSETVGSVGGHGRKDSGTAGKVGEGYTEAEPGHSGGLSSWGHTGDYEDFRVLGAFREGDFGNGTGVPLSMGPRSLERGDKEGDGERISFLGARTSTVGTGNWDKARHPGAPSPHEAGSEGHWAQVSGNALGCRDGSGIPEPWSAGDKTAYGEESKGLGPERTGPDGEVAFRGSSSSLRGMGPASEASYKSGTWGPGAVGSGFKVDCRNDLGCSGSMGSESKAGYKNKIESHGVRELGDLTGYKNDLGPSELISSRNEARGLEHSGDILSWNKAGLRDGLGETGRMESKNGVGYRGSSVGPGEMGSERKMHLADGSGRLGGPGSLAAPKEHESVGLGSVYEGAAEIGSKYNREKVRGMGFRDATGPEVGSGVAGMLDITGGIAHGDSAMGTEKLGTPDRPYILSGGQGTKNSLGGCVSLGIPDALGAVGSVGKPGIREWKDDSGFQGSLRDRGTPSEEIRSVDQAGAIGTSRLLDSRGAVEDGSGSENDFDFNKSGLETTGRCRVADQQGVTPQGCGGSLLNGRRKGTSSGSLSGVGQEVDRSSTPGRETKVMSGPGSGRATSHTRAADWEDGVQCSFGASSSLPDTDAIFRETHAGQGAFKGRGCETGQGAAGECQGPRSLGSRGSEFVGGRAGFTGSSSVPGRRDSTIYGDAVTSKPQEPQNELYSPLGRRDFKSVSGGIQEPGFQRGTGQGEGKESFQESGSWEAELDEVQGSRASAKFENQGGKDPGWSGQKPGPCGSRHQVQSGTEVGSAKRATPQGAGGLEPWAGSEDRGSLREPWSEDRRQGPHRHLGSRRDTQEGRSDVCGQAQDATQSPRSRYQPGTGRSSSEARGSMDHFSQGLTDTEVQLGEAAIVSCTLASDLGPGTWFKDGVKLTAQDGVIFEQDGLTHRLILTHVEGTQAGKYTFVAGCQHSEASLTVQDPPTIAPDVTETLREPLVFKAGKPVTVKIPFQSRLPVQAAWRKDGNEVVGGDHKGIQLALADGYTWLCLPSVCRKDSGRYSVTLRSEGGCVQAEFTLQVIDKPQPPQGPLEVQDCHRAGVCLRWLPPRDNGGQVIQHYVVERRQAGRSTWLKVGEPPSDSTSFTDTSVEQGKKYAFRVRAVTSVGAGDALESEEVLVAPEALPGPPSAPAILSASSQSITLTWGAPQGPGSNHILGYLIEKHKKGSNTWMAVNEQPVSERRYTVVGLRQGCQYEFRVTAVTLSGPGDPGPPSDAVFARDPMRPPGPVRDLQVTDTSNTSITVSWMVPDARDADEAQGYIVELCGSDSHQWSPCHVGTVPGTTFTAKGLRPQEGYFVRVTAVNDGGRSQATSLDTLVHAMPATVCPRFLMDSGTKDTLMVRVGDSIRVPLSFEAAPMPEVTWLKDGLPLPKRSVTTVKAGLTQLLIPAASLSDCGRYTVMLRNLQGKEATHSFFINVAACPQAPGSIYLQENVPGTVTVQWEPSPDEAQGIPLHYTVLMRSSSHRSWHEVADHVRTNRFTLLGVLPGHEYHFRVLAKNELGVSKPSDTSQPWCIPRQRDRFTVKSPTYQDPDLSQKPRFLVGLRAHLLPQGCECRMTCAVQGSPQPHVTWFKNDQSLDRNPAVYSTDLLGVCSLVIPSVSLKDSGEYKAVAKNPLGQAVSTATLIVTEYDS

Target ORF information:

RefSeq Version	XM_017319938.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus immunoglobulin-like and fibronectin type III domain containing 1 (Igfn1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319938.1

ORF Insert Sequence:

ATGGCTGCAA AGCCCCCTAA GAAGTCCTCC ATCCCAGGAG TGAGCATCAG GCAGCTGGTG 
GACGAGATCC CAGAAGGCTG CAGCACACCA GATTTCAAGC AGAAGCCTGT TACCTTGGCT 
CTACCAGAGG GGAAAAACGC CATCTTTCGG GCGGTGGTCT GTGGGGAACC CAGACCTGAA 
GTGCACTGGC AGAGCAGCAA AGGAGACCTC AGCAACTCCG CCAAGTACCA GATATCCTCT 
GCTCCCGGTA GAGAGGAGCA CGTGCTTCAG ATCAACAAGC TGACTGGAGA GGATTCGGAT 
CTGTACCGCT GCACTGCACT AAATGCCTAT GGAGAAGCCA CATGCTCAGT GAGGCTTACG 
GTCATTGAAG TGGGTTTTCG GAAGAACCGA AAAAGGCAAA AGGAACCTCA GGAGGACCTC 
AGGAAGGAGC TGATGGACTT TCGGAAGATG CTGAAAAAAC GGGCCCCACC CCCAACCCCT 
GAAAAAAAGA TGGAGTCTGA GCAGGTGTGG CAGCTTTTGG TGACTGCAGA CCGTAAGGAC 
TACGAGAAGA TCTGTATGCA GTATGGTATC GTCGACTTCC GGGGCATGCT GCGCAAGTTG 
CAGGAGATGA AAAAGGAACA GGAGGACAGA ATGGCCCAGT ATGTCAGTGC CATTGCCAAC 
TTGAGACACA TCAAGGTCAC CAAGGAAGGG GTCGCTACGT TTGACCTGGA GCTGGATCTC 
AAGAACCTCG AGAGCAAGAT TTACTTGTAC AAGGATGGTG AGATGATTCC CTACGGTGTT 
GATAACCAGA CCAAGCACTG TCTGAGACGG CTGGGGAAGC GCTACCACTT CCAGATCCAG 
GACCTGCGGC CTGAGGATGC TGGCCTTTAC CAGGTCATGG TGGAGGATGC TGTGGTCTTC 
TCTACAGAGC TAGAATCCAG AACCATCCCC CCCAGAGTGG TGGTCCCATT GGCAGAGACC 
CGCTGTGAGG AACGGGGTGA TGCAGTCTTT GAATGTACCC TCTCCAATCC CTGTCCCAGT 
GCGACCTGGC ATTTCCAGCA CCGACCACTG CGGCTCAGTG ACAAATATGA AGTATTTGTG 
TCCCCTGATG GGCTAACCCA CCGACTGGTT GTGAAGGGGG CCAGTAGCTC AGACATGGGC 
CTCTACTCGC TGAACACTGG GCTCCATGCC TCCAGTGCCT GGCTGGTGGT TGAAGGTGGG 
AAGGATAAAG GCCCTCAGAC CACAGATACT GACCACCGGC TGCAGACTCC AGAAGCTCTG 
GCCTCCGAAG CAGAAGACGC TGGAGGCATC AGCATCAAGG GAGGGCAATC CAGAGAGCGG 
GGTTCCCTCA AAGAGATTAG TGGGGCACAG CTCACAGCAG GCCCAGACAG GGGTGCCTTT 
GGCAAACATG GCTATCCCTT GGTGGTGGAT GAAGACATAG TTAATTCTAC TTGGGGACCT 
GGGCAGGACA AAAAGAGCTT TCTGGAAGGA GGACAACTCA GGGTCACTCT TCCAGGGGAT 
AGTCAGTCCC AAAGAGAGGG AGACTGTGGT AGAAGCCTCC CCAGAAGGTC TCATCTCCAG 
GGAGAGGATA CAGAATCAGA TTTGGGCTTC CTGGAGAGAG GACAACAAGG GCCTGGTAGA 
GTTGACAGTG AGAATGGCAG ATGGGAGACA GCAGGAGGCC AGAGTGCTGG CTCCAGCCAT 
CCTCGGGACA GAAGGCTTGA GAGCAGAGGA GAAGGACAGG AACACAGTGA GGGCCATGGC 
AGTGAACTGG ACAGATATGG CCAGGGACAA TGCCATGATC CTCAGCTGGG AGCAGGGGCA 
GGGCAGAGGG TGCCGTGGGG ATCCCAGTTT GATGCTGAGG GGCTTCAGCT GAAAAAGGAG 
GGGACAGAAA TGTGGGGCGA TTGTCTGGAT GTGACGGAAG GTAGGGGTAA CCTGAGAAAG 
GAGAGGGGAA CAGCAGCAGG GTCAAGTAAC AGAGCCCGCC TCTGGGAAGC TCGAGGAGGC 
AGTGGGGCCG GAAGTCCTTG CGTCCCTGAG TTTCCTAGAG GAGGAGCTCA CAGCTCCAAG 
GCAGGCATGG GCCCTGAGTG CTGGCCAGGT GGAGATGCTG GCCATGGAGA AGCTGGGGTA 
TACTGGGGGG CAGGGGGATA TCCAGGACAG ACATCTGAGG GCAATGACAC CCAGAAATCA 
TCACTATCTG GAGACAGGAA ACTTCTGGGG TGCACGAGTC CTGAGGCCAA AGCTGAGGGC 
TCTTTCCAGA GCGCAGATGG TCATGGGATT GTGGGGAGGG TCTACAAACC TGGCCCTGAA 
GGCCCAGAAG ATCTTAGGAG TCAGAAAAGT GGCCTACAAG AGATTCAGGG AAGGAATGGT 
CAAGGCTCAG CTGGAGCCTT GGGCAGGACG GTGGAAGGTT CCAGAAGCCT CCAATTTCCT 
CAGAGCTGGA CAGTGGGACA GAGGGAAGCA GGGAAGCCAG GTGGAGCAGA GTATGAAGAC 
ATAGGTCCTC AGGATGACAC GTGGTCCAAT CTCAGAGAGA TGGGAGGCCA CTGTGGGTCT 
GGAGTGCTGG CGTCTGGTGG GAGGGAAGGG AGCGTGGGCG GTTCCCAAGT AGCTGCCCTG 
ATGATGTCTA GTCAGAGGGT AGATGCCAGA AACCACAGGC TAGTTACATC CCCAGGCCTG 
GGTGTCCAGG GTTCTGGCAG AACACTGGGA CACATGGAAG GATTAAGAGA CCCAGAGGCA 
GTGGGATCCG AAGAAGAGTT CTGGAATGGG TCAGGAAGGT CCCAAGGAAA AGGGTCCAGA 
GGTGGGATGG GCTTAGGAGG TCCAGAGTGG GCAGAGTCTA GGAATGAGGA GGGATGGGAG 
AATTCTGAAG GGATGGCTTC TAGGAATGGA GCCACTTGTC GTGATGGATC CAGGATGCAG 
GAAGGAACAG GATCTGGCCC TGATATTGGT TGCAAGGCCC CCTGTGGGGC ACCTGCGGAG 
ACAGAGTCTG GGAAGTGGGG TACTTACAGT CATGACTCAG GGGTGCCTGG GGGATTGTGG 
TCTGGAAGCA AAAGTCAGTG TAGTCCTGCA GGAAAGGTAT CTGGAAGAGA GGCCAGCCTC 
AGAAATGGCT CAGCTGGCCT TCATGGAATG CCTTCTAGTG AAGCTCAGCA GAGGGATGTA 
TTTCAAGGCC ACGGTCAGAC AGGCCACATT GGAGAGCACC ACTGTGCCCG TGGCTTAGGG 
AGTTCTGAGA CAGTGGGATC TGTGGGTGGA CATGGTAGGA AAGACTCTGG AACAGCGGGC 
AAGGTTGGAG AGGGATACAC AGAAGCAGAA CCAGGGCACT CTGGAGGGTT GAGTTCTTGG 
GGTCACACTG GAGATTATGA GGACTTCCGA GTCCTGGGGG CCTTTAGGGA AGGAGACTTT 
GGCAATGGCA CTGGGGTTCC ATTATCCATG GGACCAAGGT CTCTGGAGAG AGGGGACAAG 
GAAGGAGATG GAGAAAGGAT CAGTTTTCTT GGTGCCAGGA CCTCTACAGT TGGAACTGGG 
AATTGGGACA AAGCCAGGCA CCCTGGGGCA CCATCTCCTC ATGAGGCCGG TTCAGAGGGC 
CACTGGGCTC AAGTGTCTGG CAATGCATTA GGTTGTAGAG ATGGGTCTGG AATTCCAGAG 
CCTTGGAGTG CTGGCGACAA AACAGCTTAT GGAGAAGAAT CAAAGGGTCT TGGGCCTGAA 
AGAACAGGAC CAGATGGTGA GGTGGCATTT AGAGGTAGCT CTTCGAGCCT CAGAGGAATG 
GGACCAGCAA GTGAGGCAAG TTATAAAAGT GGAACTTGGG GCCCTGGGGC TGTGGGTTCA 
GGGTTTAAGG TAGATTGTAG GAATGATCTT GGGTGTTCTG GAAGTATGGG GTCAGAGAGT 
AAAGCAGGTT ATAAGAACAA AATTGAGAGT CATGGGGTCA GGGAGTTAGG GGACCTGACA 
GGCTATAAAA ATGATTTAGG ACCTTCTGAA CTTATTTCAT CAAGGAATGA GGCACGTGGT 
TTAGAACATT CTGGAGACAT TTTGTCATGG AATAAGGCAG GTTTAAGGGA TGGCTTGGGA 
GAAACAGGAA GAATGGAGTC CAAGAATGGG GTTGGTTATA GGGGAAGCTC AGTGGGTCCT 
GGGGAAATGG GGTCAGAGAG GAAAATGCAC CTTGCAGATG GTTCAGGGAG GCTAGGAGGA 
CCAGGGTCAC TGGCTGCGCC CAAAGAACAT GAATCTGTGG GTCTTGGGTC AGTTTATGAG 
GGAGCAGCAG AGATTGGCTC CAAATATAAT AGGGAGAAAG TCAGAGGAAT GGGGTTTAGA 
GATGCAACAG GGCCTGAGGT GGGCTCTGGG GTAGCTGGAA TGCTGGATAT TACAGGTGGC 
ATAGCACACG GGGACAGTGC TATGGGCACA GAGAAGCTGG GGACACCAGA TAGGCCTTAT 
ATCTTGTCAG GTGGGCAGGG TACCAAGAAC AGTCTTGGGG GCTGTGTGTC CTTGGGAATC 
CCTGACGCCT TGGGGGCTGT TGGCTCTGTG GGGAAACCAG GTATCAGAGA ATGGAAAGAT 
GATTCTGGGT TCCAAGGGTC TCTCAGGGAC AGAGGGACTC CGAGTGAGGA GATCAGATCT 
GTAGACCAAG CAGGAGCTAT AGGGACATCT CGGCTTCTTG ATAGTAGGGG GGCAGTGGAG 
GATGGGAGTG GGTCAGAAAA TGACTTTGAC TTTAATAAAT CAGGCTTGGA GACAACAGGA 
AGATGTAGAG TTGCTGACCA GCAGGGAGTG ACACCACAGG GATGTGGAGG CTCATTGCTC 
AATGGCAGGA GGAAGGGAAC AAGTTCTGGA AGCTTGTCAG GGGTAGGGCA GGAGGTAGAC 
AGAAGTTCTA CACCTGGGAG GGAGACCAAG GTAATGTCAG GACCTGGCAG TGGGCGAGCC 
ACAAGCCACA CTCGGGCTGC AGACTGGGAA GACGGGGTTC AATGCAGCTT CGGGGCTTCT 
AGCTCACTGC CTGATACTGA TGCCATTTTC AGAGAAACCC ATGCGGGACA AGGAGCCTTC 
AAGGGCAGGG GCTGTGAAAC CGGCCAAGGG GCTGCTGGAG AGTGTCAAGG ACCACGCTCC 
TTGGGGAGCA GAGGCTCAGA GTTTGTAGGG GGCAGGGCTG GATTTACAGG GAGCTCAAGT 
GTTCCAGGCA GAAGGGATTC CACTATCTAC GGAGATGCTG TCACATCAAA GCCACAGGAA 
CCCCAGAATG AATTATATAG TCCTTTAGGC AGAAGGGACT TCAAAAGTGT ATCGGGAGGA 
ATCCAGGAGC CAGGTTTTCA ACGAGGAACA GGACAGGGAG AAGGAAAAGA ATCCTTCCAG 
GAGTCAGGGT CCTGGGAGGC TGAGCTTGAC GAGGTCCAAG GATCCAGGGC CTCAGCAAAG 
TTTGAAAACC AGGGAGGGAA AGACCCTGGC TGGTCAGGCC AGAAGCCTGG TCCCTGTGGA 
AGCAGGCATC AGGTGCAGTC TGGGACTGAA GTTGGATCAG CAAAGAGAGC AACCCCACAG 
GGGGCCGGAG GGCTGGAGCC TTGGGCTGGC AGTGAGGATA GAGGGTCTCT GAGAGAACCT 
TGGAGTGAAG ACAGGAGGCA AGGCCCCCAC AGGCACCTTG GCAGCAGGAG AGATACCCAA 
GAGGGCAGGT CAGATGTCTG TGGCCAGGCA CAAGATGCTA CCCAGAGCCC CAGATCCAGA 
TACCAGCCTG GCACTGGCCG CTCTTCTTCA GAGGCCCGAG GCTCCATGGA CCACTTCTCT 
CAGGGTCTGA CTGATACAGA GGTGCAACTG GGGGAAGCCG CCATAGTCTC CTGTACTCTT 
GCCAGTGACC TGGGACCTGG CACGTGGTTC AAGGATGGAG TCAAGCTCAC TGCCCAAGAT 
GGAGTCATCT TTGAGCAAGA TGGGCTCACG CACAGACTGA TTCTCACCCA CGTGGAGGGG 
ACCCAGGCTG GGAAATACAC TTTTGTAGCC GGCTGCCAGC ACAGCGAGGC CAGCCTGACC 
GTGCAGGATC CCCCCACCAT TGCTCCAGAT GTGACAGAGA CACTGAGAGA GCCACTGGTG 
TTCAAGGCTG GGAAGCCAGT CACCGTGAAA ATCCCTTTCC AGAGCCGCCT CCCTGTTCAG 
GCTGCCTGGA GGAAAGATGG GAACGAGGTG GTGGGCGGCG ACCACAAGGG CATCCAACTG 
GCTCTGGCAG ATGGCTACAC ATGGCTGTGC CTCCCCAGCG TGTGCAGGAA GGACAGTGGC 
CGGTACAGCG TGACGCTGAG GAGCGAGGGA GGCTGTGTGC AGGCTGAGTT CACCCTGCAA 
GTTATAGACA AGCCTCAGCC CCCACAAGGA CCCCTGGAGG TTCAGGATTG CCACAGAGCA 
GGTGTCTGCC TCCGCTGGCT ACCCCCGAGG GACAACGGGG GCCAGGTCAT ACAACACTAT 
GTGGTGGAGA GGCGACAGGC TGGAAGGAGC ACTTGGTTGA AGGTGGGCGA GCCCCCATCA 
GACAGTACCA GCTTCACCGA CACCAGCGTG GAGCAGGGAA AGAAATATGC CTTCCGTGTG 
CGGGCTGTGA CCTCAGTGGG AGCCGGAGAT GCCCTGGAGT CAGAGGAGGT GTTGGTGGCT 
CCTGAGGCTC TCCCGGGGCC CCCTTCTGCC CCAGCCATCC TGTCAGCCTC CAGTCAGAGC 
ATCACTCTCA CATGGGGGGC ACCACAGGGC CCTGGCAGTA ACCACATCCT GGGCTACCTG 
ATCGAGAAGC ACAAGAAGGG GAGCAACACC TGGATGGCTG TGAATGAGCA GCCCGTGTCT 
GAAAGAAGGT ACACTGTGGT GGGTCTGCGA CAGGGCTGCC AGTATGAGTT CCGGGTCACG 
GCCGTGACTC TGTCGGGCCC TGGAGATCCT GGGCCTCCAT CGGATGCTGT CTTTGCTAGA 
GACCCTATGA GACCCCCAGG GCCTGTGCGG GATCTACAGG TTACAGACAC ATCAAACACT 
AGCATCACCG TGAGCTGGAT GGTGCCGGAT GCCCGGGATG CTGATGAAGC CCAGGGATAT 
ATTGTGGAGT TGTGTGGTTC AGACAGCCAT CAATGGAGCC CATGTCACGT GGGAACTGTG 
CCGGGCACCA CCTTCACAGC CAAGGGGCTT CGGCCGCAAG AAGGCTACTT TGTGCGGGTG 
ACAGCAGTTA ATGATGGGGG CCGGAGCCAG GCCACTTCCC TGGACACATT GGTTCATGCC 
ATGCCGGCCA CTGTCTGTCC CAGGTTCCTC ATGGACTCCG GCACAAAGGA TACCCTGATG 
GTCAGGGTTG GGGACAGTAT TCGCGTTCCT CTCTCCTTTG AAGCTGCCCC CATGCCTGAG 
GTGACCTGGC TGAAGGACGG CTTGCCCTTG CCCAAAAGAA GTGTGACCAC TGTGAAGGCT 
GGCCTCACGC AGCTTCTGAT CCCTGCAGCT AGCCTCTCGG ACTGTGGCCG ATACACCGTG 
ATGCTGAGGA ACCTACAGGG GAAGGAAGCT ACCCACAGCT TCTTCATCAA TGTAGCAGCA 
TGCCCACAGG CACCAGGTTC TATCTACCTA CAGGAGAATG TGCCTGGGAC AGTGACGGTC 
CAGTGGGAAC CCTCTCCGGA TGAGGCTCAG GGCATCCCCT TGCATTACAC AGTGCTGATG 
CGGTCCTCAT CTCACCGATC CTGGCACGAG GTGGCCGACC ATGTCCGTAC CAACCGCTTT 
ACCCTCCTAG GGGTCCTCCC TGGCCATGAG TACCACTTTC GGGTGCTGGC CAAGAATGAG 
CTGGGGGTCA GCAAACCTTC AGACACCAGC CAACCCTGGT GTATCCCCAG GCAGAGAGAC 
AGGTTCACAG TGAAGTCTCC GACATACCAG GATCCTGACC TGAGCCAGAA GCCCCGCTTC 
CTGGTGGGTC TGCGGGCTCA CCTTCTGCCC CAGGGCTGTG AGTGCCGCAT GACGTGTGCC 
GTGCAGGGCT CACCCCAGCC TCATGTCACC TGGTTCAAGA ATGACCAAAG CTTGGACAGA 
AACCCTGCGG TGTACAGTAC TGACTTGCTG GGTGTGTGCT CCCTCGTCAT CCCCAGCGTG 
TCCCTTAAGG ACAGTGGTGA ATACAAGGCT GTGGCCAAGA ACCCACTGGG ACAAGCTGTC 
AGCACAGCCA CCCTCATTGT CACAGAATAC GACTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Igfn1 ( XM_017319940.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175429.1 Mus musculus immunoglobulin-like and fibronectin type III domain containing 1 (Igfn1), transcript variant X3, mRNA.

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CloneID	OMu77088
Clone ID Related Accession (Same CDS sequence)	XM_017319938.1 , XM_017319940.1 , XM_017319937.1
Accession Version	XM_017319940.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8079bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	immunoglobulin-like and fibronectin type III domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCAA AGCCCCCTAA GAAGTCCTCC ATCCCAGGAG TGAGCATCAG GCAGCTGGTG 
GACGAGATCC CAGAAGGCTG CAGCACACCA GATTTCAAGC AGAAGCCTGT TACCTTGGCT 
CTACCAGAGG GGAAAAACGC CATCTTTCGG GCGGTGGTCT GTGGGGAACC CAGACCTGAA 
GTGCACTGGC AGAGCAGCAA AGGAGACCTC AGCAACTCCG CCAAGTACCA GATATCCTCT 
GCTCCCGGTA GAGAGGAGCA CGTGCTTCAG ATCAACAAGC TGACTGGAGA GGATTCGGAT 
CTGTACCGCT GCACTGCACT AAATGCCTAT GGAGAAGCCA CATGCTCAGT GAGGCTTACG 
GTCATTGAAG TGGGTTTTCG GAAGAACCGA AAAAGGCAAA AGGAACCTCA GGAGGACCTC 
AGGAAGGAGC TGATGGACTT TCGGAAGATG CTGAAAAAAC GGGCCCCACC CCCAACCCCT 
GAAAAAAAGA TGGAGTCTGA GCAGGTGTGG CAGCTTTTGG TGACTGCAGA CCGTAAGGAC 
TACGAGAAGA TCTGTATGCA GTATGGTATC GTCGACTTCC GGGGCATGCT GCGCAAGTTG 
CAGGAGATGA AAAAGGAACA GGAGGACAGA ATGGCCCAGT ATGTCAGTGC CATTGCCAAC 
TTGAGACACA TCAAGGTCAC CAAGGAAGGG GTCGCTACGT TTGACCTGGA GCTGGATCTC 
AAGAACCTCG AGAGCAAGAT TTACTTGTAC AAGGATGGTG AGATGATTCC CTACGGTGTT 
GATAACCAGA CCAAGCACTG TCTGAGACGG CTGGGGAAGC GCTACCACTT CCAGATCCAG 
GACCTGCGGC CTGAGGATGC TGGCCTTTAC CAGGTCATGG TGGAGGATGC TGTGGTCTTC 
TCTACAGAGC TAGAATCCAG AACCATCCCC CCCAGAGTGG TGGTCCCATT GGCAGAGACC 
CGCTGTGAGG AACGGGGTGA TGCAGTCTTT GAATGTACCC TCTCCAATCC CTGTCCCAGT 
GCGACCTGGC ATTTCCAGCA CCGACCACTG CGGCTCAGTG ACAAATATGA AGTATTTGTG 
TCCCCTGATG GGCTAACCCA CCGACTGGTT GTGAAGGGGG CCAGTAGCTC AGACATGGGC 
CTCTACTCGC TGAACACTGG GCTCCATGCC TCCAGTGCCT GGCTGGTGGT TGAAGGTGGG 
AAGGATAAAG GCCCTCAGAC CACAGATACT GACCACCGGC TGCAGACTCC AGAAGCTCTG 
GCCTCCGAAG CAGAAGACGC TGGAGGCATC AGCATCAAGG GAGGGCAATC CAGAGAGCGG 
GGTTCCCTCA AAGAGATTAG TGGGGCACAG CTCACAGCAG GCCCAGACAG GGGTGCCTTT 
GGCAAACATG GCTATCCCTT GGTGGTGGAT GAAGACATAG TTAATTCTAC TTGGGGACCT 
GGGCAGGACA AAAAGAGCTT TCTGGAAGGA GGACAACTCA GGGTCACTCT TCCAGGGGAT 
AGTCAGTCCC AAAGAGAGGG AGACTGTGGT AGAAGCCTCC CCAGAAGGTC TCATCTCCAG 
GGAGAGGATA CAGAATCAGA TTTGGGCTTC CTGGAGAGAG GACAACAAGG GCCTGGTAGA 
GTTGACAGTG AGAATGGCAG ATGGGAGACA GCAGGAGGCC AGAGTGCTGG CTCCAGCCAT 
CCTCGGGACA GAAGGCTTGA GAGCAGAGGA GAAGGACAGG AACACAGTGA GGGCCATGGC 
AGTGAACTGG ACAGATATGG CCAGGGACAA TGCCATGATC CTCAGCTGGG AGCAGGGGCA 
GGGCAGAGGG TGCCGTGGGG ATCCCAGTTT GATGCTGAGG GGCTTCAGCT GAAAAAGGAG 
GGGACAGAAA TGTGGGGCGA TTGTCTGGAT GTGACGGAAG GTAGGGGTAA CCTGAGAAAG 
GAGAGGGGAA CAGCAGCAGG GTCAAGTAAC AGAGCCCGCC TCTGGGAAGC TCGAGGAGGC 
AGTGGGGCCG GAAGTCCTTG CGTCCCTGAG TTTCCTAGAG GAGGAGCTCA CAGCTCCAAG 
GCAGGCATGG GCCCTGAGTG CTGGCCAGGT GGAGATGCTG GCCATGGAGA AGCTGGGGTA 
TACTGGGGGG CAGGGGGATA TCCAGGACAG ACATCTGAGG GCAATGACAC CCAGAAATCA 
TCACTATCTG GAGACAGGAA ACTTCTGGGG TGCACGAGTC CTGAGGCCAA AGCTGAGGGC 
TCTTTCCAGA GCGCAGATGG TCATGGGATT GTGGGGAGGG TCTACAAACC TGGCCCTGAA 
GGCCCAGAAG ATCTTAGGAG TCAGAAAAGT GGCCTACAAG AGATTCAGGG AAGGAATGGT 
CAAGGCTCAG CTGGAGCCTT GGGCAGGACG GTGGAAGGTT CCAGAAGCCT CCAATTTCCT 
CAGAGCTGGA CAGTGGGACA GAGGGAAGCA GGGAAGCCAG GTGGAGCAGA GTATGAAGAC 
ATAGGTCCTC AGGATGACAC GTGGTCCAAT CTCAGAGAGA TGGGAGGCCA CTGTGGGTCT 
GGAGTGCTGG CGTCTGGTGG GAGGGAAGGG AGCGTGGGCG GTTCCCAAGT AGCTGCCCTG 
ATGATGTCTA GTCAGAGGGT AGATGCCAGA AACCACAGGC TAGTTACATC CCCAGGCCTG 
GGTGTCCAGG GTTCTGGCAG AACACTGGGA CACATGGAAG GATTAAGAGA CCCAGAGGCA 
GTGGGATCCG AAGAAGAGTT CTGGAATGGG TCAGGAAGGT CCCAAGGAAA AGGGTCCAGA 
GGTGGGATGG GCTTAGGAGG TCCAGAGTGG GCAGAGTCTA GGAATGAGGA GGGATGGGAG 
AATTCTGAAG GGATGGCTTC TAGGAATGGA GCCACTTGTC GTGATGGATC CAGGATGCAG 
GAAGGAACAG GATCTGGCCC TGATATTGGT TGCAAGGCCC CCTGTGGGGC ACCTGCGGAG 
ACAGAGTCTG GGAAGTGGGG TACTTACAGT CATGACTCAG GGGTGCCTGG GGGATTGTGG 
TCTGGAAGCA AAAGTCAGTG TAGTCCTGCA GGAAAGGTAT CTGGAAGAGA GGCCAGCCTC 
AGAAATGGCT CAGCTGGCCT TCATGGAATG CCTTCTAGTG AAGCTCAGCA GAGGGATGTA 
TTTCAAGGCC ACGGTCAGAC AGGCCACATT GGAGAGCACC ACTGTGCCCG TGGCTTAGGG 
AGTTCTGAGA CAGTGGGATC TGTGGGTGGA CATGGTAGGA AAGACTCTGG AACAGCGGGC 
AAGGTTGGAG AGGGATACAC AGAAGCAGAA CCAGGGCACT CTGGAGGGTT GAGTTCTTGG 
GGTCACACTG GAGATTATGA GGACTTCCGA GTCCTGGGGG CCTTTAGGGA AGGAGACTTT 
GGCAATGGCA CTGGGGTTCC ATTATCCATG GGACCAAGGT CTCTGGAGAG AGGGGACAAG 
GAAGGAGATG GAGAAAGGAT CAGTTTTCTT GGTGCCAGGA CCTCTACAGT TGGAACTGGG 
AATTGGGACA AAGCCAGGCA CCCTGGGGCA CCATCTCCTC ATGAGGCCGG TTCAGAGGGC 
CACTGGGCTC AAGTGTCTGG CAATGCATTA GGTTGTAGAG ATGGGTCTGG AATTCCAGAG 
CCTTGGAGTG CTGGCGACAA AACAGCTTAT GGAGAAGAAT CAAAGGGTCT TGGGCCTGAA 
AGAACAGGAC CAGATGGTGA GGTGGCATTT AGAGGTAGCT CTTCGAGCCT CAGAGGAATG 
GGACCAGCAA GTGAGGCAAG TTATAAAAGT GGAACTTGGG GCCCTGGGGC TGTGGGTTCA 
GGGTTTAAGG TAGATTGTAG GAATGATCTT GGGTGTTCTG GAAGTATGGG GTCAGAGAGT 
AAAGCAGGTT ATAAGAACAA AATTGAGAGT CATGGGGTCA GGGAGTTAGG GGACCTGACA 
GGCTATAAAA ATGATTTAGG ACCTTCTGAA CTTATTTCAT CAAGGAATGA GGCACGTGGT 
TTAGAACATT CTGGAGACAT TTTGTCATGG AATAAGGCAG GTTTAAGGGA TGGCTTGGGA 
GAAACAGGAA GAATGGAGTC CAAGAATGGG GTTGGTTATA GGGGAAGCTC AGTGGGTCCT 
GGGGAAATGG GGTCAGAGAG GAAAATGCAC CTTGCAGATG GTTCAGGGAG GCTAGGAGGA 
CCAGGGTCAC TGGCTGCGCC CAAAGAACAT GAATCTGTGG GTCTTGGGTC AGTTTATGAG 
GGAGCAGCAG AGATTGGCTC CAAATATAAT AGGGAGAAAG TCAGAGGAAT GGGGTTTAGA 
GATGCAACAG GGCCTGAGGT GGGCTCTGGG GTAGCTGGAA TGCTGGATAT TACAGGTGGC 
ATAGCACACG GGGACAGTGC TATGGGCACA GAGAAGCTGG GGACACCAGA TAGGCCTTAT 
ATCTTGTCAG GTGGGCAGGG TACCAAGAAC AGTCTTGGGG GCTGTGTGTC CTTGGGAATC 
CCTGACGCCT TGGGGGCTGT TGGCTCTGTG GGGAAACCAG GTATCAGAGA ATGGAAAGAT 
GATTCTGGGT TCCAAGGGTC TCTCAGGGAC AGAGGGACTC CGAGTGAGGA GATCAGATCT 
GTAGACCAAG CAGGAGCTAT AGGGACATCT CGGCTTCTTG ATAGTAGGGG GGCAGTGGAG 
GATGGGAGTG GGTCAGAAAA TGACTTTGAC TTTAATAAAT CAGGCTTGGA GACAACAGGA 
AGATGTAGAG TTGCTGACCA GCAGGGAGTG ACACCACAGG GATGTGGAGG CTCATTGCTC 
AATGGCAGGA GGAAGGGAAC AAGTTCTGGA AGCTTGTCAG GGGTAGGGCA GGAGGTAGAC 
AGAAGTTCTA CACCTGGGAG GGAGACCAAG GTAATGTCAG GACCTGGCAG TGGGCGAGCC 
ACAAGCCACA CTCGGGCTGC AGACTGGGAA GACGGGGTTC AATGCAGCTT CGGGGCTTCT 
AGCTCACTGC CTGATACTGA TGCCATTTTC AGAGAAACCC ATGCGGGACA AGGAGCCTTC 
AAGGGCAGGG GCTGTGAAAC CGGCCAAGGG GCTGCTGGAG AGTGTCAAGG ACCACGCTCC 
TTGGGGAGCA GAGGCTCAGA GTTTGTAGGG GGCAGGGCTG GATTTACAGG GAGCTCAAGT 
GTTCCAGGCA GAAGGGATTC CACTATCTAC GGAGATGCTG TCACATCAAA GCCACAGGAA 
CCCCAGAATG AATTATATAG TCCTTTAGGC AGAAGGGACT TCAAAAGTGT ATCGGGAGGA 
ATCCAGGAGC CAGGTTTTCA ACGAGGAACA GGACAGGGAG AAGGAAAAGA ATCCTTCCAG 
GAGTCAGGGT CCTGGGAGGC TGAGCTTGAC GAGGTCCAAG GATCCAGGGC CTCAGCAAAG 
TTTGAAAACC AGGGAGGGAA AGACCCTGGC TGGTCAGGCC AGAAGCCTGG TCCCTGTGGA 
AGCAGGCATC AGGTGCAGTC TGGGACTGAA GTTGGATCAG CAAAGAGAGC AACCCCACAG 
GGGGCCGGAG GGCTGGAGCC TTGGGCTGGC AGTGAGGATA GAGGGTCTCT GAGAGAACCT 
TGGAGTGAAG ACAGGAGGCA AGGCCCCCAC AGGCACCTTG GCAGCAGGAG AGATACCCAA 
GAGGGCAGGT CAGATGTCTG TGGCCAGGCA CAAGATGCTA CCCAGAGCCC CAGATCCAGA 
TACCAGCCTG GCACTGGCCG CTCTTCTTCA GAGGCCCGAG GCTCCATGGA CCACTTCTCT 
CAGGGTCTGA CTGATACAGA GGTGCAACTG GGGGAAGCCG CCATAGTCTC CTGTACTCTT 
GCCAGTGACC TGGGACCTGG CACGTGGTTC AAGGATGGAG TCAAGCTCAC TGCCCAAGAT 
GGAGTCATCT TTGAGCAAGA TGGGCTCACG CACAGACTGA TTCTCACCCA CGTGGAGGGG 
ACCCAGGCTG GGAAATACAC TTTTGTAGCC GGCTGCCAGC ACAGCGAGGC CAGCCTGACC 
GTGCAGGATC CCCCCACCAT TGCTCCAGAT GTGACAGAGA CACTGAGAGA GCCACTGGTG 
TTCAAGGCTG GGAAGCCAGT CACCGTGAAA ATCCCTTTCC AGAGCCGCCT CCCTGTTCAG 
GCTGCCTGGA GGAAAGATGG GAACGAGGTG GTGGGCGGCG ACCACAAGGG CATCCAACTG 
GCTCTGGCAG ATGGCTACAC ATGGCTGTGC CTCCCCAGCG TGTGCAGGAA GGACAGTGGC 
CGGTACAGCG TGACGCTGAG GAGCGAGGGA GGCTGTGTGC AGGCTGAGTT CACCCTGCAA 
GTTATAGACA AGCCTCAGCC CCCACAAGGA CCCCTGGAGG TTCAGGATTG CCACAGAGCA 
GGTGTCTGCC TCCGCTGGCT ACCCCCGAGG GACAACGGGG GCCAGGTCAT ACAACACTAT 
GTGGTGGAGA GGCGACAGGC TGGAAGGAGC ACTTGGTTGA AGGTGGGCGA GCCCCCATCA 
GACAGTACCA GCTTCACCGA CACCAGCGTG GAGCAGGGAA AGAAATATGC CTTCCGTGTG 
CGGGCTGTGA CCTCAGTGGG AGCCGGAGAT GCCCTGGAGT CAGAGGAGGT GTTGGTGGCT 
CCTGAGGCTC TCCCGGGGCC CCCTTCTGCC CCAGCCATCC TGTCAGCCTC CAGTCAGAGC 
ATCACTCTCA CATGGGGGGC ACCACAGGGC CCTGGCAGTA ACCACATCCT GGGCTACCTG 
ATCGAGAAGC ACAAGAAGGG GAGCAACACC TGGATGGCTG TGAATGAGCA GCCCGTGTCT 
GAAAGAAGGT ACACTGTGGT GGGTCTGCGA CAGGGCTGCC AGTATGAGTT CCGGGTCACG 
GCCGTGACTC TGTCGGGCCC TGGAGATCCT GGGCCTCCAT CGGATGCTGT CTTTGCTAGA 
GACCCTATGA GACCCCCAGG GCCTGTGCGG GATCTACAGG TTACAGACAC ATCAAACACT 
AGCATCACCG TGAGCTGGAT GGTGCCGGAT GCCCGGGATG CTGATGAAGC CCAGGGATAT 
ATTGTGGAGT TGTGTGGTTC AGACAGCCAT CAATGGAGCC CATGTCACGT GGGAACTGTG 
CCGGGCACCA CCTTCACAGC CAAGGGGCTT CGGCCGCAAG AAGGCTACTT TGTGCGGGTG 
ACAGCAGTTA ATGATGGGGG CCGGAGCCAG GCCACTTCCC TGGACACATT GGTTCATGCC 
ATGCCGGCCA CTGTCTGTCC CAGGTTCCTC ATGGACTCCG GCACAAAGGA TACCCTGATG 
GTCAGGGTTG GGGACAGTAT TCGCGTTCCT CTCTCCTTTG AAGCTGCCCC CATGCCTGAG 
GTGACCTGGC TGAAGGACGG CTTGCCCTTG CCCAAAAGAA GTGTGACCAC TGTGAAGGCT 
GGCCTCACGC AGCTTCTGAT CCCTGCAGCT AGCCTCTCGG ACTGTGGCCG ATACACCGTG 
ATGCTGAGGA ACCTACAGGG GAAGGAAGCT ACCCACAGCT TCTTCATCAA TGTAGCAGCA 
TGCCCACAGG CACCAGGTTC TATCTACCTA CAGGAGAATG TGCCTGGGAC AGTGACGGTC 
CAGTGGGAAC CCTCTCCGGA TGAGGCTCAG GGCATCCCCT TGCATTACAC AGTGCTGATG 
CGGTCCTCAT CTCACCGATC CTGGCACGAG GTGGCCGACC ATGTCCGTAC CAACCGCTTT 
ACCCTCCTAG GGGTCCTCCC TGGCCATGAG TACCACTTTC GGGTGCTGGC CAAGAATGAG 
CTGGGGGTCA GCAAACCTTC AGACACCAGC CAACCCTGGT GTATCCCCAG GCAGAGAGAC 
AGGTTCACAG TGAAGTCTCC GACATACCAG GATCCTGACC TGAGCCAGAA GCCCCGCTTC 
CTGGTGGGTC TGCGGGCTCA CCTTCTGCCC CAGGGCTGTG AGTGCCGCAT GACGTGTGCC 
GTGCAGGGCT CACCCCAGCC TCATGTCACC TGGTTCAAGA ATGACCAAAG CTTGGACAGA 
AACCCTGCGG TGTACAGTAC TGACTTGCTG GGTGTGTGCT CCCTCGTCAT CCCCAGCGTG 
TCCCTTAAGG ACAGTGGTGA ATACAAGGCT GTGGCCAAGA ACCCACTGGG ACAAGCTGTC 
AGCACAGCCA CCCTCATTGT CACAGAATAC GACTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175429.1
CDS	504..8582
Translation	MAAKPPKKSSIPGVSIRQLVDEIPEGCSTPDFKQKPVTLALPEGKNAIFRAVVCGEPRPEVHWQSSKGDLSNSAKYQISSAPGREEHVLQINKLTGEDSDLYRCTALNAYGEATCSVRLTVIEVGFRKNRKRQKEPQEDLRKELMDFRKMLKKRAPPPTPEKKMESEQVWQLLVTADRKDYEKICMQYGIVDFRGMLRKLQEMKKEQEDRMAQYVSAIANLRHIKVTKEGVATFDLELDLKNLESKIYLYKDGEMIPYGVDNQTKHCLRRLGKRYHFQIQDLRPEDAGLYQVMVEDAVVFSTELESRTIPPRVVVPLAETRCEERGDAVFECTLSNPCPSATWHFQHRPLRLSDKYEVFVSPDGLTHRLVVKGASSSDMGLYSLNTGLHASSAWLVVEGGKDKGPQTTDTDHRLQTPEALASEAEDAGGISIKGGQSRERGSLKEISGAQLTAGPDRGAFGKHGYPLVVDEDIVNSTWGPGQDKKSFLEGGQLRVTLPGDSQSQREGDCGRSLPRRSHLQGEDTESDLGFLERGQQGPGRVDSENGRWETAGGQSAGSSHPRDRRLESRGEGQEHSEGHGSELDRYGQGQCHDPQLGAGAGQRVPWGSQFDAEGLQLKKEGTEMWGDCLDVTEGRGNLRKERGTAAGSSNRARLWEARGGSGAGSPCVPEFPRGGAHSSKAGMGPECWPGGDAGHGEAGVYWGAGGYPGQTSEGNDTQKSSLSGDRKLLGCTSPEAKAEGSFQSADGHGIVGRVYKPGPEGPEDLRSQKSGLQEIQGRNGQGSAGALGRTVEGSRSLQFPQSWTVGQREAGKPGGAEYEDIGPQDDTWSNLREMGGHCGSGVLASGGREGSVGGSQVAALMMSSQRVDARNHRLVTSPGLGVQGSGRTLGHMEGLRDPEAVGSEEEFWNGSGRSQGKGSRGGMGLGGPEWAESRNEEGWENSEGMASRNGATCRDGSRMQEGTGSGPDIGCKAPCGAPAETESGKWGTYSHDSGVPGGLWSGSKSQCSPAGKVSGREASLRNGSAGLHGMPSSEAQQRDVFQGHGQTGHIGEHHCARGLGSSETVGSVGGHGRKDSGTAGKVGEGYTEAEPGHSGGLSSWGHTGDYEDFRVLGAFREGDFGNGTGVPLSMGPRSLERGDKEGDGERISFLGARTSTVGTGNWDKARHPGAPSPHEAGSEGHWAQVSGNALGCRDGSGIPEPWSAGDKTAYGEESKGLGPERTGPDGEVAFRGSSSSLRGMGPASEASYKSGTWGPGAVGSGFKVDCRNDLGCSGSMGSESKAGYKNKIESHGVRELGDLTGYKNDLGPSELISSRNEARGLEHSGDILSWNKAGLRDGLGETGRMESKNGVGYRGSSVGPGEMGSERKMHLADGSGRLGGPGSLAAPKEHESVGLGSVYEGAAEIGSKYNREKVRGMGFRDATGPEVGSGVAGMLDITGGIAHGDSAMGTEKLGTPDRPYILSGGQGTKNSLGGCVSLGIPDALGAVGSVGKPGIREWKDDSGFQGSLRDRGTPSEEIRSVDQAGAIGTSRLLDSRGAVEDGSGSENDFDFNKSGLETTGRCRVADQQGVTPQGCGGSLLNGRRKGTSSGSLSGVGQEVDRSSTPGRETKVMSGPGSGRATSHTRAADWEDGVQCSFGASSSLPDTDAIFRETHAGQGAFKGRGCETGQGAAGECQGPRSLGSRGSEFVGGRAGFTGSSSVPGRRDSTIYGDAVTSKPQEPQNELYSPLGRRDFKSVSGGIQEPGFQRGTGQGEGKESFQESGSWEAELDEVQGSRASAKFENQGGKDPGWSGQKPGPCGSRHQVQSGTEVGSAKRATPQGAGGLEPWAGSEDRGSLREPWSEDRRQGPHRHLGSRRDTQEGRSDVCGQAQDATQSPRSRYQPGTGRSSSEARGSMDHFSQGLTDTEVQLGEAAIVSCTLASDLGPGTWFKDGVKLTAQDGVIFEQDGLTHRLILTHVEGTQAGKYTFVAGCQHSEASLTVQDPPTIAPDVTETLREPLVFKAGKPVTVKIPFQSRLPVQAAWRKDGNEVVGGDHKGIQLALADGYTWLCLPSVCRKDSGRYSVTLRSEGGCVQAEFTLQVIDKPQPPQGPLEVQDCHRAGVCLRWLPPRDNGGQVIQHYVVERRQAGRSTWLKVGEPPSDSTSFTDTSVEQGKKYAFRVRAVTSVGAGDALESEEVLVAPEALPGPPSAPAILSASSQSITLTWGAPQGPGSNHILGYLIEKHKKGSNTWMAVNEQPVSERRYTVVGLRQGCQYEFRVTAVTLSGPGDPGPPSDAVFARDPMRPPGPVRDLQVTDTSNTSITVSWMVPDARDADEAQGYIVELCGSDSHQWSPCHVGTVPGTTFTAKGLRPQEGYFVRVTAVNDGGRSQATSLDTLVHAMPATVCPRFLMDSGTKDTLMVRVGDSIRVPLSFEAAPMPEVTWLKDGLPLPKRSVTTVKAGLTQLLIPAASLSDCGRYTVMLRNLQGKEATHSFFINVAACPQAPGSIYLQENVPGTVTVQWEPSPDEAQGIPLHYTVLMRSSSHRSWHEVADHVRTNRFTLLGVLPGHEYHFRVLAKNELGVSKPSDTSQPWCIPRQRDRFTVKSPTYQDPDLSQKPRFLVGLRAHLLPQGCECRMTCAVQGSPQPHVTWFKNDQSLDRNPAVYSTDLLGVCSLVIPSVSLKDSGEYKAVAKNPLGQAVSTATLIVTEYDS

Target ORF information:

RefSeq Version	XM_017319940.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus immunoglobulin-like and fibronectin type III domain containing 1 (Igfn1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319940.1

ORF Insert Sequence:

ATGGCTGCAA AGCCCCCTAA GAAGTCCTCC ATCCCAGGAG TGAGCATCAG GCAGCTGGTG 
GACGAGATCC CAGAAGGCTG CAGCACACCA GATTTCAAGC AGAAGCCTGT TACCTTGGCT 
CTACCAGAGG GGAAAAACGC CATCTTTCGG GCGGTGGTCT GTGGGGAACC CAGACCTGAA 
GTGCACTGGC AGAGCAGCAA AGGAGACCTC AGCAACTCCG CCAAGTACCA GATATCCTCT 
GCTCCCGGTA GAGAGGAGCA CGTGCTTCAG ATCAACAAGC TGACTGGAGA GGATTCGGAT 
CTGTACCGCT GCACTGCACT AAATGCCTAT GGAGAAGCCA CATGCTCAGT GAGGCTTACG 
GTCATTGAAG TGGGTTTTCG GAAGAACCGA AAAAGGCAAA AGGAACCTCA GGAGGACCTC 
AGGAAGGAGC TGATGGACTT TCGGAAGATG CTGAAAAAAC GGGCCCCACC CCCAACCCCT 
GAAAAAAAGA TGGAGTCTGA GCAGGTGTGG CAGCTTTTGG TGACTGCAGA CCGTAAGGAC 
TACGAGAAGA TCTGTATGCA GTATGGTATC GTCGACTTCC GGGGCATGCT GCGCAAGTTG 
CAGGAGATGA AAAAGGAACA GGAGGACAGA ATGGCCCAGT ATGTCAGTGC CATTGCCAAC 
TTGAGACACA TCAAGGTCAC CAAGGAAGGG GTCGCTACGT TTGACCTGGA GCTGGATCTC 
AAGAACCTCG AGAGCAAGAT TTACTTGTAC AAGGATGGTG AGATGATTCC CTACGGTGTT 
GATAACCAGA CCAAGCACTG TCTGAGACGG CTGGGGAAGC GCTACCACTT CCAGATCCAG 
GACCTGCGGC CTGAGGATGC TGGCCTTTAC CAGGTCATGG TGGAGGATGC TGTGGTCTTC 
TCTACAGAGC TAGAATCCAG AACCATCCCC CCCAGAGTGG TGGTCCCATT GGCAGAGACC 
CGCTGTGAGG AACGGGGTGA TGCAGTCTTT GAATGTACCC TCTCCAATCC CTGTCCCAGT 
GCGACCTGGC ATTTCCAGCA CCGACCACTG CGGCTCAGTG ACAAATATGA AGTATTTGTG 
TCCCCTGATG GGCTAACCCA CCGACTGGTT GTGAAGGGGG CCAGTAGCTC AGACATGGGC 
CTCTACTCGC TGAACACTGG GCTCCATGCC TCCAGTGCCT GGCTGGTGGT TGAAGGTGGG 
AAGGATAAAG GCCCTCAGAC CACAGATACT GACCACCGGC TGCAGACTCC AGAAGCTCTG 
GCCTCCGAAG CAGAAGACGC TGGAGGCATC AGCATCAAGG GAGGGCAATC CAGAGAGCGG 
GGTTCCCTCA AAGAGATTAG TGGGGCACAG CTCACAGCAG GCCCAGACAG GGGTGCCTTT 
GGCAAACATG GCTATCCCTT GGTGGTGGAT GAAGACATAG TTAATTCTAC TTGGGGACCT 
GGGCAGGACA AAAAGAGCTT TCTGGAAGGA GGACAACTCA GGGTCACTCT TCCAGGGGAT 
AGTCAGTCCC AAAGAGAGGG AGACTGTGGT AGAAGCCTCC CCAGAAGGTC TCATCTCCAG 
GGAGAGGATA CAGAATCAGA TTTGGGCTTC CTGGAGAGAG GACAACAAGG GCCTGGTAGA 
GTTGACAGTG AGAATGGCAG ATGGGAGACA GCAGGAGGCC AGAGTGCTGG CTCCAGCCAT 
CCTCGGGACA GAAGGCTTGA GAGCAGAGGA GAAGGACAGG AACACAGTGA GGGCCATGGC 
AGTGAACTGG ACAGATATGG CCAGGGACAA TGCCATGATC CTCAGCTGGG AGCAGGGGCA 
GGGCAGAGGG TGCCGTGGGG ATCCCAGTTT GATGCTGAGG GGCTTCAGCT GAAAAAGGAG 
GGGACAGAAA TGTGGGGCGA TTGTCTGGAT GTGACGGAAG GTAGGGGTAA CCTGAGAAAG 
GAGAGGGGAA CAGCAGCAGG GTCAAGTAAC AGAGCCCGCC TCTGGGAAGC TCGAGGAGGC 
AGTGGGGCCG GAAGTCCTTG CGTCCCTGAG TTTCCTAGAG GAGGAGCTCA CAGCTCCAAG 
GCAGGCATGG GCCCTGAGTG CTGGCCAGGT GGAGATGCTG GCCATGGAGA AGCTGGGGTA 
TACTGGGGGG CAGGGGGATA TCCAGGACAG ACATCTGAGG GCAATGACAC CCAGAAATCA 
TCACTATCTG GAGACAGGAA ACTTCTGGGG TGCACGAGTC CTGAGGCCAA AGCTGAGGGC 
TCTTTCCAGA GCGCAGATGG TCATGGGATT GTGGGGAGGG TCTACAAACC TGGCCCTGAA 
GGCCCAGAAG ATCTTAGGAG TCAGAAAAGT GGCCTACAAG AGATTCAGGG AAGGAATGGT 
CAAGGCTCAG CTGGAGCCTT GGGCAGGACG GTGGAAGGTT CCAGAAGCCT CCAATTTCCT 
CAGAGCTGGA CAGTGGGACA GAGGGAAGCA GGGAAGCCAG GTGGAGCAGA GTATGAAGAC 
ATAGGTCCTC AGGATGACAC GTGGTCCAAT CTCAGAGAGA TGGGAGGCCA CTGTGGGTCT 
GGAGTGCTGG CGTCTGGTGG GAGGGAAGGG AGCGTGGGCG GTTCCCAAGT AGCTGCCCTG 
ATGATGTCTA GTCAGAGGGT AGATGCCAGA AACCACAGGC TAGTTACATC CCCAGGCCTG 
GGTGTCCAGG GTTCTGGCAG AACACTGGGA CACATGGAAG GATTAAGAGA CCCAGAGGCA 
GTGGGATCCG AAGAAGAGTT CTGGAATGGG TCAGGAAGGT CCCAAGGAAA AGGGTCCAGA 
GGTGGGATGG GCTTAGGAGG TCCAGAGTGG GCAGAGTCTA GGAATGAGGA GGGATGGGAG 
AATTCTGAAG GGATGGCTTC TAGGAATGGA GCCACTTGTC GTGATGGATC CAGGATGCAG 
GAAGGAACAG GATCTGGCCC TGATATTGGT TGCAAGGCCC CCTGTGGGGC ACCTGCGGAG 
ACAGAGTCTG GGAAGTGGGG TACTTACAGT CATGACTCAG GGGTGCCTGG GGGATTGTGG 
TCTGGAAGCA AAAGTCAGTG TAGTCCTGCA GGAAAGGTAT CTGGAAGAGA GGCCAGCCTC 
AGAAATGGCT CAGCTGGCCT TCATGGAATG CCTTCTAGTG AAGCTCAGCA GAGGGATGTA 
TTTCAAGGCC ACGGTCAGAC AGGCCACATT GGAGAGCACC ACTGTGCCCG TGGCTTAGGG 
AGTTCTGAGA CAGTGGGATC TGTGGGTGGA CATGGTAGGA AAGACTCTGG AACAGCGGGC 
AAGGTTGGAG AGGGATACAC AGAAGCAGAA CCAGGGCACT CTGGAGGGTT GAGTTCTTGG 
GGTCACACTG GAGATTATGA GGACTTCCGA GTCCTGGGGG CCTTTAGGGA AGGAGACTTT 
GGCAATGGCA CTGGGGTTCC ATTATCCATG GGACCAAGGT CTCTGGAGAG AGGGGACAAG 
GAAGGAGATG GAGAAAGGAT CAGTTTTCTT GGTGCCAGGA CCTCTACAGT TGGAACTGGG 
AATTGGGACA AAGCCAGGCA CCCTGGGGCA CCATCTCCTC ATGAGGCCGG TTCAGAGGGC 
CACTGGGCTC AAGTGTCTGG CAATGCATTA GGTTGTAGAG ATGGGTCTGG AATTCCAGAG 
CCTTGGAGTG CTGGCGACAA AACAGCTTAT GGAGAAGAAT CAAAGGGTCT TGGGCCTGAA 
AGAACAGGAC CAGATGGTGA GGTGGCATTT AGAGGTAGCT CTTCGAGCCT CAGAGGAATG 
GGACCAGCAA GTGAGGCAAG TTATAAAAGT GGAACTTGGG GCCCTGGGGC TGTGGGTTCA 
GGGTTTAAGG TAGATTGTAG GAATGATCTT GGGTGTTCTG GAAGTATGGG GTCAGAGAGT 
AAAGCAGGTT ATAAGAACAA AATTGAGAGT CATGGGGTCA GGGAGTTAGG GGACCTGACA 
GGCTATAAAA ATGATTTAGG ACCTTCTGAA CTTATTTCAT CAAGGAATGA GGCACGTGGT 
TTAGAACATT CTGGAGACAT TTTGTCATGG AATAAGGCAG GTTTAAGGGA TGGCTTGGGA 
GAAACAGGAA GAATGGAGTC CAAGAATGGG GTTGGTTATA GGGGAAGCTC AGTGGGTCCT 
GGGGAAATGG GGTCAGAGAG GAAAATGCAC CTTGCAGATG GTTCAGGGAG GCTAGGAGGA 
CCAGGGTCAC TGGCTGCGCC CAAAGAACAT GAATCTGTGG GTCTTGGGTC AGTTTATGAG 
GGAGCAGCAG AGATTGGCTC CAAATATAAT AGGGAGAAAG TCAGAGGAAT GGGGTTTAGA 
GATGCAACAG GGCCTGAGGT GGGCTCTGGG GTAGCTGGAA TGCTGGATAT TACAGGTGGC 
ATAGCACACG GGGACAGTGC TATGGGCACA GAGAAGCTGG GGACACCAGA TAGGCCTTAT 
ATCTTGTCAG GTGGGCAGGG TACCAAGAAC AGTCTTGGGG GCTGTGTGTC CTTGGGAATC 
CCTGACGCCT TGGGGGCTGT TGGCTCTGTG GGGAAACCAG GTATCAGAGA ATGGAAAGAT 
GATTCTGGGT TCCAAGGGTC TCTCAGGGAC AGAGGGACTC CGAGTGAGGA GATCAGATCT 
GTAGACCAAG CAGGAGCTAT AGGGACATCT CGGCTTCTTG ATAGTAGGGG GGCAGTGGAG 
GATGGGAGTG GGTCAGAAAA TGACTTTGAC TTTAATAAAT CAGGCTTGGA GACAACAGGA 
AGATGTAGAG TTGCTGACCA GCAGGGAGTG ACACCACAGG GATGTGGAGG CTCATTGCTC 
AATGGCAGGA GGAAGGGAAC AAGTTCTGGA AGCTTGTCAG GGGTAGGGCA GGAGGTAGAC 
AGAAGTTCTA CACCTGGGAG GGAGACCAAG GTAATGTCAG GACCTGGCAG TGGGCGAGCC 
ACAAGCCACA CTCGGGCTGC AGACTGGGAA GACGGGGTTC AATGCAGCTT CGGGGCTTCT 
AGCTCACTGC CTGATACTGA TGCCATTTTC AGAGAAACCC ATGCGGGACA AGGAGCCTTC 
AAGGGCAGGG GCTGTGAAAC CGGCCAAGGG GCTGCTGGAG AGTGTCAAGG ACCACGCTCC 
TTGGGGAGCA GAGGCTCAGA GTTTGTAGGG GGCAGGGCTG GATTTACAGG GAGCTCAAGT 
GTTCCAGGCA GAAGGGATTC CACTATCTAC GGAGATGCTG TCACATCAAA GCCACAGGAA 
CCCCAGAATG AATTATATAG TCCTTTAGGC AGAAGGGACT TCAAAAGTGT ATCGGGAGGA 
ATCCAGGAGC CAGGTTTTCA ACGAGGAACA GGACAGGGAG AAGGAAAAGA ATCCTTCCAG 
GAGTCAGGGT CCTGGGAGGC TGAGCTTGAC GAGGTCCAAG GATCCAGGGC CTCAGCAAAG 
TTTGAAAACC AGGGAGGGAA AGACCCTGGC TGGTCAGGCC AGAAGCCTGG TCCCTGTGGA 
AGCAGGCATC AGGTGCAGTC TGGGACTGAA GTTGGATCAG CAAAGAGAGC AACCCCACAG 
GGGGCCGGAG GGCTGGAGCC TTGGGCTGGC AGTGAGGATA GAGGGTCTCT GAGAGAACCT 
TGGAGTGAAG ACAGGAGGCA AGGCCCCCAC AGGCACCTTG GCAGCAGGAG AGATACCCAA 
GAGGGCAGGT CAGATGTCTG TGGCCAGGCA CAAGATGCTA CCCAGAGCCC CAGATCCAGA 
TACCAGCCTG GCACTGGCCG CTCTTCTTCA GAGGCCCGAG GCTCCATGGA CCACTTCTCT 
CAGGGTCTGA CTGATACAGA GGTGCAACTG GGGGAAGCCG CCATAGTCTC CTGTACTCTT 
GCCAGTGACC TGGGACCTGG CACGTGGTTC AAGGATGGAG TCAAGCTCAC TGCCCAAGAT 
GGAGTCATCT TTGAGCAAGA TGGGCTCACG CACAGACTGA TTCTCACCCA CGTGGAGGGG 
ACCCAGGCTG GGAAATACAC TTTTGTAGCC GGCTGCCAGC ACAGCGAGGC CAGCCTGACC 
GTGCAGGATC CCCCCACCAT TGCTCCAGAT GTGACAGAGA CACTGAGAGA GCCACTGGTG 
TTCAAGGCTG GGAAGCCAGT CACCGTGAAA ATCCCTTTCC AGAGCCGCCT CCCTGTTCAG 
GCTGCCTGGA GGAAAGATGG GAACGAGGTG GTGGGCGGCG ACCACAAGGG CATCCAACTG 
GCTCTGGCAG ATGGCTACAC ATGGCTGTGC CTCCCCAGCG TGTGCAGGAA GGACAGTGGC 
CGGTACAGCG TGACGCTGAG GAGCGAGGGA GGCTGTGTGC AGGCTGAGTT CACCCTGCAA 
GTTATAGACA AGCCTCAGCC CCCACAAGGA CCCCTGGAGG TTCAGGATTG CCACAGAGCA 
GGTGTCTGCC TCCGCTGGCT ACCCCCGAGG GACAACGGGG GCCAGGTCAT ACAACACTAT 
GTGGTGGAGA GGCGACAGGC TGGAAGGAGC ACTTGGTTGA AGGTGGGCGA GCCCCCATCA 
GACAGTACCA GCTTCACCGA CACCAGCGTG GAGCAGGGAA AGAAATATGC CTTCCGTGTG 
CGGGCTGTGA CCTCAGTGGG AGCCGGAGAT GCCCTGGAGT CAGAGGAGGT GTTGGTGGCT 
CCTGAGGCTC TCCCGGGGCC CCCTTCTGCC CCAGCCATCC TGTCAGCCTC CAGTCAGAGC 
ATCACTCTCA CATGGGGGGC ACCACAGGGC CCTGGCAGTA ACCACATCCT GGGCTACCTG 
ATCGAGAAGC ACAAGAAGGG GAGCAACACC TGGATGGCTG TGAATGAGCA GCCCGTGTCT 
GAAAGAAGGT ACACTGTGGT GGGTCTGCGA CAGGGCTGCC AGTATGAGTT CCGGGTCACG 
GCCGTGACTC TGTCGGGCCC TGGAGATCCT GGGCCTCCAT CGGATGCTGT CTTTGCTAGA 
GACCCTATGA GACCCCCAGG GCCTGTGCGG GATCTACAGG TTACAGACAC ATCAAACACT 
AGCATCACCG TGAGCTGGAT GGTGCCGGAT GCCCGGGATG CTGATGAAGC CCAGGGATAT 
ATTGTGGAGT TGTGTGGTTC AGACAGCCAT CAATGGAGCC CATGTCACGT GGGAACTGTG 
CCGGGCACCA CCTTCACAGC CAAGGGGCTT CGGCCGCAAG AAGGCTACTT TGTGCGGGTG 
ACAGCAGTTA ATGATGGGGG CCGGAGCCAG GCCACTTCCC TGGACACATT GGTTCATGCC 
ATGCCGGCCA CTGTCTGTCC CAGGTTCCTC ATGGACTCCG GCACAAAGGA TACCCTGATG 
GTCAGGGTTG GGGACAGTAT TCGCGTTCCT CTCTCCTTTG AAGCTGCCCC CATGCCTGAG 
GTGACCTGGC TGAAGGACGG CTTGCCCTTG CCCAAAAGAA GTGTGACCAC TGTGAAGGCT 
GGCCTCACGC AGCTTCTGAT CCCTGCAGCT AGCCTCTCGG ACTGTGGCCG ATACACCGTG 
ATGCTGAGGA ACCTACAGGG GAAGGAAGCT ACCCACAGCT TCTTCATCAA TGTAGCAGCA 
TGCCCACAGG CACCAGGTTC TATCTACCTA CAGGAGAATG TGCCTGGGAC AGTGACGGTC 
CAGTGGGAAC CCTCTCCGGA TGAGGCTCAG GGCATCCCCT TGCATTACAC AGTGCTGATG 
CGGTCCTCAT CTCACCGATC CTGGCACGAG GTGGCCGACC ATGTCCGTAC CAACCGCTTT 
ACCCTCCTAG GGGTCCTCCC TGGCCATGAG TACCACTTTC GGGTGCTGGC CAAGAATGAG 
CTGGGGGTCA GCAAACCTTC AGACACCAGC CAACCCTGGT GTATCCCCAG GCAGAGAGAC 
AGGTTCACAG TGAAGTCTCC GACATACCAG GATCCTGACC TGAGCCAGAA GCCCCGCTTC 
CTGGTGGGTC TGCGGGCTCA CCTTCTGCCC CAGGGCTGTG AGTGCCGCAT GACGTGTGCC 
GTGCAGGGCT CACCCCAGCC TCATGTCACC TGGTTCAAGA ATGACCAAAG CTTGGACAGA 
AACCCTGCGG TGTACAGTAC TGACTTGCTG GGTGTGTGCT CCCTCGTCAT CCCCAGCGTG 
TCCCTTAAGG ACAGTGGTGA ATACAAGGCT GTGGCCAAGA ACCCACTGGG ACAAGCTGTC 
AGCACAGCCA CCCTCATTGT CACAGAATAC GACTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Igfn1 ( XM_017319937.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175426.1 Mus musculus immunoglobulin-like and fibronectin type III domain containing 1 (Igfn1), transcript variant X1, mRNA.

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CloneID	OMu77088
Clone ID Related Accession (Same CDS sequence)	XM_017319938.1 , XM_017319940.1 , XM_017319937.1
Accession Version	XM_017319937.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8079bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	immunoglobulin-like and fibronectin type III domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCAA AGCCCCCTAA GAAGTCCTCC ATCCCAGGAG TGAGCATCAG GCAGCTGGTG 
GACGAGATCC CAGAAGGCTG CAGCACACCA GATTTCAAGC AGAAGCCTGT TACCTTGGCT 
CTACCAGAGG GGAAAAACGC CATCTTTCGG GCGGTGGTCT GTGGGGAACC CAGACCTGAA 
GTGCACTGGC AGAGCAGCAA AGGAGACCTC AGCAACTCCG CCAAGTACCA GATATCCTCT 
GCTCCCGGTA GAGAGGAGCA CGTGCTTCAG ATCAACAAGC TGACTGGAGA GGATTCGGAT 
CTGTACCGCT GCACTGCACT AAATGCCTAT GGAGAAGCCA CATGCTCAGT GAGGCTTACG 
GTCATTGAAG TGGGTTTTCG GAAGAACCGA AAAAGGCAAA AGGAACCTCA GGAGGACCTC 
AGGAAGGAGC TGATGGACTT TCGGAAGATG CTGAAAAAAC GGGCCCCACC CCCAACCCCT 
GAAAAAAAGA TGGAGTCTGA GCAGGTGTGG CAGCTTTTGG TGACTGCAGA CCGTAAGGAC 
TACGAGAAGA TCTGTATGCA GTATGGTATC GTCGACTTCC GGGGCATGCT GCGCAAGTTG 
CAGGAGATGA AAAAGGAACA GGAGGACAGA ATGGCCCAGT ATGTCAGTGC CATTGCCAAC 
TTGAGACACA TCAAGGTCAC CAAGGAAGGG GTCGCTACGT TTGACCTGGA GCTGGATCTC 
AAGAACCTCG AGAGCAAGAT TTACTTGTAC AAGGATGGTG AGATGATTCC CTACGGTGTT 
GATAACCAGA CCAAGCACTG TCTGAGACGG CTGGGGAAGC GCTACCACTT CCAGATCCAG 
GACCTGCGGC CTGAGGATGC TGGCCTTTAC CAGGTCATGG TGGAGGATGC TGTGGTCTTC 
TCTACAGAGC TAGAATCCAG AACCATCCCC CCCAGAGTGG TGGTCCCATT GGCAGAGACC 
CGCTGTGAGG AACGGGGTGA TGCAGTCTTT GAATGTACCC TCTCCAATCC CTGTCCCAGT 
GCGACCTGGC ATTTCCAGCA CCGACCACTG CGGCTCAGTG ACAAATATGA AGTATTTGTG 
TCCCCTGATG GGCTAACCCA CCGACTGGTT GTGAAGGGGG CCAGTAGCTC AGACATGGGC 
CTCTACTCGC TGAACACTGG GCTCCATGCC TCCAGTGCCT GGCTGGTGGT TGAAGGTGGG 
AAGGATAAAG GCCCTCAGAC CACAGATACT GACCACCGGC TGCAGACTCC AGAAGCTCTG 
GCCTCCGAAG CAGAAGACGC TGGAGGCATC AGCATCAAGG GAGGGCAATC CAGAGAGCGG 
GGTTCCCTCA AAGAGATTAG TGGGGCACAG CTCACAGCAG GCCCAGACAG GGGTGCCTTT 
GGCAAACATG GCTATCCCTT GGTGGTGGAT GAAGACATAG TTAATTCTAC TTGGGGACCT 
GGGCAGGACA AAAAGAGCTT TCTGGAAGGA GGACAACTCA GGGTCACTCT TCCAGGGGAT 
AGTCAGTCCC AAAGAGAGGG AGACTGTGGT AGAAGCCTCC CCAGAAGGTC TCATCTCCAG 
GGAGAGGATA CAGAATCAGA TTTGGGCTTC CTGGAGAGAG GACAACAAGG GCCTGGTAGA 
GTTGACAGTG AGAATGGCAG ATGGGAGACA GCAGGAGGCC AGAGTGCTGG CTCCAGCCAT 
CCTCGGGACA GAAGGCTTGA GAGCAGAGGA GAAGGACAGG AACACAGTGA GGGCCATGGC 
AGTGAACTGG ACAGATATGG CCAGGGACAA TGCCATGATC CTCAGCTGGG AGCAGGGGCA 
GGGCAGAGGG TGCCGTGGGG ATCCCAGTTT GATGCTGAGG GGCTTCAGCT GAAAAAGGAG 
GGGACAGAAA TGTGGGGCGA TTGTCTGGAT GTGACGGAAG GTAGGGGTAA CCTGAGAAAG 
GAGAGGGGAA CAGCAGCAGG GTCAAGTAAC AGAGCCCGCC TCTGGGAAGC TCGAGGAGGC 
AGTGGGGCCG GAAGTCCTTG CGTCCCTGAG TTTCCTAGAG GAGGAGCTCA CAGCTCCAAG 
GCAGGCATGG GCCCTGAGTG CTGGCCAGGT GGAGATGCTG GCCATGGAGA AGCTGGGGTA 
TACTGGGGGG CAGGGGGATA TCCAGGACAG ACATCTGAGG GCAATGACAC CCAGAAATCA 
TCACTATCTG GAGACAGGAA ACTTCTGGGG TGCACGAGTC CTGAGGCCAA AGCTGAGGGC 
TCTTTCCAGA GCGCAGATGG TCATGGGATT GTGGGGAGGG TCTACAAACC TGGCCCTGAA 
GGCCCAGAAG ATCTTAGGAG TCAGAAAAGT GGCCTACAAG AGATTCAGGG AAGGAATGGT 
CAAGGCTCAG CTGGAGCCTT GGGCAGGACG GTGGAAGGTT CCAGAAGCCT CCAATTTCCT 
CAGAGCTGGA CAGTGGGACA GAGGGAAGCA GGGAAGCCAG GTGGAGCAGA GTATGAAGAC 
ATAGGTCCTC AGGATGACAC GTGGTCCAAT CTCAGAGAGA TGGGAGGCCA CTGTGGGTCT 
GGAGTGCTGG CGTCTGGTGG GAGGGAAGGG AGCGTGGGCG GTTCCCAAGT AGCTGCCCTG 
ATGATGTCTA GTCAGAGGGT AGATGCCAGA AACCACAGGC TAGTTACATC CCCAGGCCTG 
GGTGTCCAGG GTTCTGGCAG AACACTGGGA CACATGGAAG GATTAAGAGA CCCAGAGGCA 
GTGGGATCCG AAGAAGAGTT CTGGAATGGG TCAGGAAGGT CCCAAGGAAA AGGGTCCAGA 
GGTGGGATGG GCTTAGGAGG TCCAGAGTGG GCAGAGTCTA GGAATGAGGA GGGATGGGAG 
AATTCTGAAG GGATGGCTTC TAGGAATGGA GCCACTTGTC GTGATGGATC CAGGATGCAG 
GAAGGAACAG GATCTGGCCC TGATATTGGT TGCAAGGCCC CCTGTGGGGC ACCTGCGGAG 
ACAGAGTCTG GGAAGTGGGG TACTTACAGT CATGACTCAG GGGTGCCTGG GGGATTGTGG 
TCTGGAAGCA AAAGTCAGTG TAGTCCTGCA GGAAAGGTAT CTGGAAGAGA GGCCAGCCTC 
AGAAATGGCT CAGCTGGCCT TCATGGAATG CCTTCTAGTG AAGCTCAGCA GAGGGATGTA 
TTTCAAGGCC ACGGTCAGAC AGGCCACATT GGAGAGCACC ACTGTGCCCG TGGCTTAGGG 
AGTTCTGAGA CAGTGGGATC TGTGGGTGGA CATGGTAGGA AAGACTCTGG AACAGCGGGC 
AAGGTTGGAG AGGGATACAC AGAAGCAGAA CCAGGGCACT CTGGAGGGTT GAGTTCTTGG 
GGTCACACTG GAGATTATGA GGACTTCCGA GTCCTGGGGG CCTTTAGGGA AGGAGACTTT 
GGCAATGGCA CTGGGGTTCC ATTATCCATG GGACCAAGGT CTCTGGAGAG AGGGGACAAG 
GAAGGAGATG GAGAAAGGAT CAGTTTTCTT GGTGCCAGGA CCTCTACAGT TGGAACTGGG 
AATTGGGACA AAGCCAGGCA CCCTGGGGCA CCATCTCCTC ATGAGGCCGG TTCAGAGGGC 
CACTGGGCTC AAGTGTCTGG CAATGCATTA GGTTGTAGAG ATGGGTCTGG AATTCCAGAG 
CCTTGGAGTG CTGGCGACAA AACAGCTTAT GGAGAAGAAT CAAAGGGTCT TGGGCCTGAA 
AGAACAGGAC CAGATGGTGA GGTGGCATTT AGAGGTAGCT CTTCGAGCCT CAGAGGAATG 
GGACCAGCAA GTGAGGCAAG TTATAAAAGT GGAACTTGGG GCCCTGGGGC TGTGGGTTCA 
GGGTTTAAGG TAGATTGTAG GAATGATCTT GGGTGTTCTG GAAGTATGGG GTCAGAGAGT 
AAAGCAGGTT ATAAGAACAA AATTGAGAGT CATGGGGTCA GGGAGTTAGG GGACCTGACA 
GGCTATAAAA ATGATTTAGG ACCTTCTGAA CTTATTTCAT CAAGGAATGA GGCACGTGGT 
TTAGAACATT CTGGAGACAT TTTGTCATGG AATAAGGCAG GTTTAAGGGA TGGCTTGGGA 
GAAACAGGAA GAATGGAGTC CAAGAATGGG GTTGGTTATA GGGGAAGCTC AGTGGGTCCT 
GGGGAAATGG GGTCAGAGAG GAAAATGCAC CTTGCAGATG GTTCAGGGAG GCTAGGAGGA 
CCAGGGTCAC TGGCTGCGCC CAAAGAACAT GAATCTGTGG GTCTTGGGTC AGTTTATGAG 
GGAGCAGCAG AGATTGGCTC CAAATATAAT AGGGAGAAAG TCAGAGGAAT GGGGTTTAGA 
GATGCAACAG GGCCTGAGGT GGGCTCTGGG GTAGCTGGAA TGCTGGATAT TACAGGTGGC 
ATAGCACACG GGGACAGTGC TATGGGCACA GAGAAGCTGG GGACACCAGA TAGGCCTTAT 
ATCTTGTCAG GTGGGCAGGG TACCAAGAAC AGTCTTGGGG GCTGTGTGTC CTTGGGAATC 
CCTGACGCCT TGGGGGCTGT TGGCTCTGTG GGGAAACCAG GTATCAGAGA ATGGAAAGAT 
GATTCTGGGT TCCAAGGGTC TCTCAGGGAC AGAGGGACTC CGAGTGAGGA GATCAGATCT 
GTAGACCAAG CAGGAGCTAT AGGGACATCT CGGCTTCTTG ATAGTAGGGG GGCAGTGGAG 
GATGGGAGTG GGTCAGAAAA TGACTTTGAC TTTAATAAAT CAGGCTTGGA GACAACAGGA 
AGATGTAGAG TTGCTGACCA GCAGGGAGTG ACACCACAGG GATGTGGAGG CTCATTGCTC 
AATGGCAGGA GGAAGGGAAC AAGTTCTGGA AGCTTGTCAG GGGTAGGGCA GGAGGTAGAC 
AGAAGTTCTA CACCTGGGAG GGAGACCAAG GTAATGTCAG GACCTGGCAG TGGGCGAGCC 
ACAAGCCACA CTCGGGCTGC AGACTGGGAA GACGGGGTTC AATGCAGCTT CGGGGCTTCT 
AGCTCACTGC CTGATACTGA TGCCATTTTC AGAGAAACCC ATGCGGGACA AGGAGCCTTC 
AAGGGCAGGG GCTGTGAAAC CGGCCAAGGG GCTGCTGGAG AGTGTCAAGG ACCACGCTCC 
TTGGGGAGCA GAGGCTCAGA GTTTGTAGGG GGCAGGGCTG GATTTACAGG GAGCTCAAGT 
GTTCCAGGCA GAAGGGATTC CACTATCTAC GGAGATGCTG TCACATCAAA GCCACAGGAA 
CCCCAGAATG AATTATATAG TCCTTTAGGC AGAAGGGACT TCAAAAGTGT ATCGGGAGGA 
ATCCAGGAGC CAGGTTTTCA ACGAGGAACA GGACAGGGAG AAGGAAAAGA ATCCTTCCAG 
GAGTCAGGGT CCTGGGAGGC TGAGCTTGAC GAGGTCCAAG GATCCAGGGC CTCAGCAAAG 
TTTGAAAACC AGGGAGGGAA AGACCCTGGC TGGTCAGGCC AGAAGCCTGG TCCCTGTGGA 
AGCAGGCATC AGGTGCAGTC TGGGACTGAA GTTGGATCAG CAAAGAGAGC AACCCCACAG 
GGGGCCGGAG GGCTGGAGCC TTGGGCTGGC AGTGAGGATA GAGGGTCTCT GAGAGAACCT 
TGGAGTGAAG ACAGGAGGCA AGGCCCCCAC AGGCACCTTG GCAGCAGGAG AGATACCCAA 
GAGGGCAGGT CAGATGTCTG TGGCCAGGCA CAAGATGCTA CCCAGAGCCC CAGATCCAGA 
TACCAGCCTG GCACTGGCCG CTCTTCTTCA GAGGCCCGAG GCTCCATGGA CCACTTCTCT 
CAGGGTCTGA CTGATACAGA GGTGCAACTG GGGGAAGCCG CCATAGTCTC CTGTACTCTT 
GCCAGTGACC TGGGACCTGG CACGTGGTTC AAGGATGGAG TCAAGCTCAC TGCCCAAGAT 
GGAGTCATCT TTGAGCAAGA TGGGCTCACG CACAGACTGA TTCTCACCCA CGTGGAGGGG 
ACCCAGGCTG GGAAATACAC TTTTGTAGCC GGCTGCCAGC ACAGCGAGGC CAGCCTGACC 
GTGCAGGATC CCCCCACCAT TGCTCCAGAT GTGACAGAGA CACTGAGAGA GCCACTGGTG 
TTCAAGGCTG GGAAGCCAGT CACCGTGAAA ATCCCTTTCC AGAGCCGCCT CCCTGTTCAG 
GCTGCCTGGA GGAAAGATGG GAACGAGGTG GTGGGCGGCG ACCACAAGGG CATCCAACTG 
GCTCTGGCAG ATGGCTACAC ATGGCTGTGC CTCCCCAGCG TGTGCAGGAA GGACAGTGGC 
CGGTACAGCG TGACGCTGAG GAGCGAGGGA GGCTGTGTGC AGGCTGAGTT CACCCTGCAA 
GTTATAGACA AGCCTCAGCC CCCACAAGGA CCCCTGGAGG TTCAGGATTG CCACAGAGCA 
GGTGTCTGCC TCCGCTGGCT ACCCCCGAGG GACAACGGGG GCCAGGTCAT ACAACACTAT 
GTGGTGGAGA GGCGACAGGC TGGAAGGAGC ACTTGGTTGA AGGTGGGCGA GCCCCCATCA 
GACAGTACCA GCTTCACCGA CACCAGCGTG GAGCAGGGAA AGAAATATGC CTTCCGTGTG 
CGGGCTGTGA CCTCAGTGGG AGCCGGAGAT GCCCTGGAGT CAGAGGAGGT GTTGGTGGCT 
CCTGAGGCTC TCCCGGGGCC CCCTTCTGCC CCAGCCATCC TGTCAGCCTC CAGTCAGAGC 
ATCACTCTCA CATGGGGGGC ACCACAGGGC CCTGGCAGTA ACCACATCCT GGGCTACCTG 
ATCGAGAAGC ACAAGAAGGG GAGCAACACC TGGATGGCTG TGAATGAGCA GCCCGTGTCT 
GAAAGAAGGT ACACTGTGGT GGGTCTGCGA CAGGGCTGCC AGTATGAGTT CCGGGTCACG 
GCCGTGACTC TGTCGGGCCC TGGAGATCCT GGGCCTCCAT CGGATGCTGT CTTTGCTAGA 
GACCCTATGA GACCCCCAGG GCCTGTGCGG GATCTACAGG TTACAGACAC ATCAAACACT 
AGCATCACCG TGAGCTGGAT GGTGCCGGAT GCCCGGGATG CTGATGAAGC CCAGGGATAT 
ATTGTGGAGT TGTGTGGTTC AGACAGCCAT CAATGGAGCC CATGTCACGT GGGAACTGTG 
CCGGGCACCA CCTTCACAGC CAAGGGGCTT CGGCCGCAAG AAGGCTACTT TGTGCGGGTG 
ACAGCAGTTA ATGATGGGGG CCGGAGCCAG GCCACTTCCC TGGACACATT GGTTCATGCC 
ATGCCGGCCA CTGTCTGTCC CAGGTTCCTC ATGGACTCCG GCACAAAGGA TACCCTGATG 
GTCAGGGTTG GGGACAGTAT TCGCGTTCCT CTCTCCTTTG AAGCTGCCCC CATGCCTGAG 
GTGACCTGGC TGAAGGACGG CTTGCCCTTG CCCAAAAGAA GTGTGACCAC TGTGAAGGCT 
GGCCTCACGC AGCTTCTGAT CCCTGCAGCT AGCCTCTCGG ACTGTGGCCG ATACACCGTG 
ATGCTGAGGA ACCTACAGGG GAAGGAAGCT ACCCACAGCT TCTTCATCAA TGTAGCAGCA 
TGCCCACAGG CACCAGGTTC TATCTACCTA CAGGAGAATG TGCCTGGGAC AGTGACGGTC 
CAGTGGGAAC CCTCTCCGGA TGAGGCTCAG GGCATCCCCT TGCATTACAC AGTGCTGATG 
CGGTCCTCAT CTCACCGATC CTGGCACGAG GTGGCCGACC ATGTCCGTAC CAACCGCTTT 
ACCCTCCTAG GGGTCCTCCC TGGCCATGAG TACCACTTTC GGGTGCTGGC CAAGAATGAG 
CTGGGGGTCA GCAAACCTTC AGACACCAGC CAACCCTGGT GTATCCCCAG GCAGAGAGAC 
AGGTTCACAG TGAAGTCTCC GACATACCAG GATCCTGACC TGAGCCAGAA GCCCCGCTTC 
CTGGTGGGTC TGCGGGCTCA CCTTCTGCCC CAGGGCTGTG AGTGCCGCAT GACGTGTGCC 
GTGCAGGGCT CACCCCAGCC TCATGTCACC TGGTTCAAGA ATGACCAAAG CTTGGACAGA 
AACCCTGCGG TGTACAGTAC TGACTTGCTG GGTGTGTGCT CCCTCGTCAT CCCCAGCGTG 
TCCCTTAAGG ACAGTGGTGA ATACAAGGCT GTGGCCAAGA ACCCACTGGG ACAAGCTGTC 
AGCACAGCCA CCCTCATTGT CACAGAATAC GACTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175426.1
CDS	567..8645
Translation	MAAKPPKKSSIPGVSIRQLVDEIPEGCSTPDFKQKPVTLALPEGKNAIFRAVVCGEPRPEVHWQSSKGDLSNSAKYQISSAPGREEHVLQINKLTGEDSDLYRCTALNAYGEATCSVRLTVIEVGFRKNRKRQKEPQEDLRKELMDFRKMLKKRAPPPTPEKKMESEQVWQLLVTADRKDYEKICMQYGIVDFRGMLRKLQEMKKEQEDRMAQYVSAIANLRHIKVTKEGVATFDLELDLKNLESKIYLYKDGEMIPYGVDNQTKHCLRRLGKRYHFQIQDLRPEDAGLYQVMVEDAVVFSTELESRTIPPRVVVPLAETRCEERGDAVFECTLSNPCPSATWHFQHRPLRLSDKYEVFVSPDGLTHRLVVKGASSSDMGLYSLNTGLHASSAWLVVEGGKDKGPQTTDTDHRLQTPEALASEAEDAGGISIKGGQSRERGSLKEISGAQLTAGPDRGAFGKHGYPLVVDEDIVNSTWGPGQDKKSFLEGGQLRVTLPGDSQSQREGDCGRSLPRRSHLQGEDTESDLGFLERGQQGPGRVDSENGRWETAGGQSAGSSHPRDRRLESRGEGQEHSEGHGSELDRYGQGQCHDPQLGAGAGQRVPWGSQFDAEGLQLKKEGTEMWGDCLDVTEGRGNLRKERGTAAGSSNRARLWEARGGSGAGSPCVPEFPRGGAHSSKAGMGPECWPGGDAGHGEAGVYWGAGGYPGQTSEGNDTQKSSLSGDRKLLGCTSPEAKAEGSFQSADGHGIVGRVYKPGPEGPEDLRSQKSGLQEIQGRNGQGSAGALGRTVEGSRSLQFPQSWTVGQREAGKPGGAEYEDIGPQDDTWSNLREMGGHCGSGVLASGGREGSVGGSQVAALMMSSQRVDARNHRLVTSPGLGVQGSGRTLGHMEGLRDPEAVGSEEEFWNGSGRSQGKGSRGGMGLGGPEWAESRNEEGWENSEGMASRNGATCRDGSRMQEGTGSGPDIGCKAPCGAPAETESGKWGTYSHDSGVPGGLWSGSKSQCSPAGKVSGREASLRNGSAGLHGMPSSEAQQRDVFQGHGQTGHIGEHHCARGLGSSETVGSVGGHGRKDSGTAGKVGEGYTEAEPGHSGGLSSWGHTGDYEDFRVLGAFREGDFGNGTGVPLSMGPRSLERGDKEGDGERISFLGARTSTVGTGNWDKARHPGAPSPHEAGSEGHWAQVSGNALGCRDGSGIPEPWSAGDKTAYGEESKGLGPERTGPDGEVAFRGSSSSLRGMGPASEASYKSGTWGPGAVGSGFKVDCRNDLGCSGSMGSESKAGYKNKIESHGVRELGDLTGYKNDLGPSELISSRNEARGLEHSGDILSWNKAGLRDGLGETGRMESKNGVGYRGSSVGPGEMGSERKMHLADGSGRLGGPGSLAAPKEHESVGLGSVYEGAAEIGSKYNREKVRGMGFRDATGPEVGSGVAGMLDITGGIAHGDSAMGTEKLGTPDRPYILSGGQGTKNSLGGCVSLGIPDALGAVGSVGKPGIREWKDDSGFQGSLRDRGTPSEEIRSVDQAGAIGTSRLLDSRGAVEDGSGSENDFDFNKSGLETTGRCRVADQQGVTPQGCGGSLLNGRRKGTSSGSLSGVGQEVDRSSTPGRETKVMSGPGSGRATSHTRAADWEDGVQCSFGASSSLPDTDAIFRETHAGQGAFKGRGCETGQGAAGECQGPRSLGSRGSEFVGGRAGFTGSSSVPGRRDSTIYGDAVTSKPQEPQNELYSPLGRRDFKSVSGGIQEPGFQRGTGQGEGKESFQESGSWEAELDEVQGSRASAKFENQGGKDPGWSGQKPGPCGSRHQVQSGTEVGSAKRATPQGAGGLEPWAGSEDRGSLREPWSEDRRQGPHRHLGSRRDTQEGRSDVCGQAQDATQSPRSRYQPGTGRSSSEARGSMDHFSQGLTDTEVQLGEAAIVSCTLASDLGPGTWFKDGVKLTAQDGVIFEQDGLTHRLILTHVEGTQAGKYTFVAGCQHSEASLTVQDPPTIAPDVTETLREPLVFKAGKPVTVKIPFQSRLPVQAAWRKDGNEVVGGDHKGIQLALADGYTWLCLPSVCRKDSGRYSVTLRSEGGCVQAEFTLQVIDKPQPPQGPLEVQDCHRAGVCLRWLPPRDNGGQVIQHYVVERRQAGRSTWLKVGEPPSDSTSFTDTSVEQGKKYAFRVRAVTSVGAGDALESEEVLVAPEALPGPPSAPAILSASSQSITLTWGAPQGPGSNHILGYLIEKHKKGSNTWMAVNEQPVSERRYTVVGLRQGCQYEFRVTAVTLSGPGDPGPPSDAVFARDPMRPPGPVRDLQVTDTSNTSITVSWMVPDARDADEAQGYIVELCGSDSHQWSPCHVGTVPGTTFTAKGLRPQEGYFVRVTAVNDGGRSQATSLDTLVHAMPATVCPRFLMDSGTKDTLMVRVGDSIRVPLSFEAAPMPEVTWLKDGLPLPKRSVTTVKAGLTQLLIPAASLSDCGRYTVMLRNLQGKEATHSFFINVAACPQAPGSIYLQENVPGTVTVQWEPSPDEAQGIPLHYTVLMRSSSHRSWHEVADHVRTNRFTLLGVLPGHEYHFRVLAKNELGVSKPSDTSQPWCIPRQRDRFTVKSPTYQDPDLSQKPRFLVGLRAHLLPQGCECRMTCAVQGSPQPHVTWFKNDQSLDRNPAVYSTDLLGVCSLVIPSVSLKDSGEYKAVAKNPLGQAVSTATLIVTEYDS

Target ORF information:

RefSeq Version	XM_017319937.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus immunoglobulin-like and fibronectin type III domain containing 1 (Igfn1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319937.1

ORF Insert Sequence:

ATGGCTGCAA AGCCCCCTAA GAAGTCCTCC ATCCCAGGAG TGAGCATCAG GCAGCTGGTG 
GACGAGATCC CAGAAGGCTG CAGCACACCA GATTTCAAGC AGAAGCCTGT TACCTTGGCT 
CTACCAGAGG GGAAAAACGC CATCTTTCGG GCGGTGGTCT GTGGGGAACC CAGACCTGAA 
GTGCACTGGC AGAGCAGCAA AGGAGACCTC AGCAACTCCG CCAAGTACCA GATATCCTCT 
GCTCCCGGTA GAGAGGAGCA CGTGCTTCAG ATCAACAAGC TGACTGGAGA GGATTCGGAT 
CTGTACCGCT GCACTGCACT AAATGCCTAT GGAGAAGCCA CATGCTCAGT GAGGCTTACG 
GTCATTGAAG TGGGTTTTCG GAAGAACCGA AAAAGGCAAA AGGAACCTCA GGAGGACCTC 
AGGAAGGAGC TGATGGACTT TCGGAAGATG CTGAAAAAAC GGGCCCCACC CCCAACCCCT 
GAAAAAAAGA TGGAGTCTGA GCAGGTGTGG CAGCTTTTGG TGACTGCAGA CCGTAAGGAC 
TACGAGAAGA TCTGTATGCA GTATGGTATC GTCGACTTCC GGGGCATGCT GCGCAAGTTG 
CAGGAGATGA AAAAGGAACA GGAGGACAGA ATGGCCCAGT ATGTCAGTGC CATTGCCAAC 
TTGAGACACA TCAAGGTCAC CAAGGAAGGG GTCGCTACGT TTGACCTGGA GCTGGATCTC 
AAGAACCTCG AGAGCAAGAT TTACTTGTAC AAGGATGGTG AGATGATTCC CTACGGTGTT 
GATAACCAGA CCAAGCACTG TCTGAGACGG CTGGGGAAGC GCTACCACTT CCAGATCCAG 
GACCTGCGGC CTGAGGATGC TGGCCTTTAC CAGGTCATGG TGGAGGATGC TGTGGTCTTC 
TCTACAGAGC TAGAATCCAG AACCATCCCC CCCAGAGTGG TGGTCCCATT GGCAGAGACC 
CGCTGTGAGG AACGGGGTGA TGCAGTCTTT GAATGTACCC TCTCCAATCC CTGTCCCAGT 
GCGACCTGGC ATTTCCAGCA CCGACCACTG CGGCTCAGTG ACAAATATGA AGTATTTGTG 
TCCCCTGATG GGCTAACCCA CCGACTGGTT GTGAAGGGGG CCAGTAGCTC AGACATGGGC 
CTCTACTCGC TGAACACTGG GCTCCATGCC TCCAGTGCCT GGCTGGTGGT TGAAGGTGGG 
AAGGATAAAG GCCCTCAGAC CACAGATACT GACCACCGGC TGCAGACTCC AGAAGCTCTG 
GCCTCCGAAG CAGAAGACGC TGGAGGCATC AGCATCAAGG GAGGGCAATC CAGAGAGCGG 
GGTTCCCTCA AAGAGATTAG TGGGGCACAG CTCACAGCAG GCCCAGACAG GGGTGCCTTT 
GGCAAACATG GCTATCCCTT GGTGGTGGAT GAAGACATAG TTAATTCTAC TTGGGGACCT 
GGGCAGGACA AAAAGAGCTT TCTGGAAGGA GGACAACTCA GGGTCACTCT TCCAGGGGAT 
AGTCAGTCCC AAAGAGAGGG AGACTGTGGT AGAAGCCTCC CCAGAAGGTC TCATCTCCAG 
GGAGAGGATA CAGAATCAGA TTTGGGCTTC CTGGAGAGAG GACAACAAGG GCCTGGTAGA 
GTTGACAGTG AGAATGGCAG ATGGGAGACA GCAGGAGGCC AGAGTGCTGG CTCCAGCCAT 
CCTCGGGACA GAAGGCTTGA GAGCAGAGGA GAAGGACAGG AACACAGTGA GGGCCATGGC 
AGTGAACTGG ACAGATATGG CCAGGGACAA TGCCATGATC CTCAGCTGGG AGCAGGGGCA 
GGGCAGAGGG TGCCGTGGGG ATCCCAGTTT GATGCTGAGG GGCTTCAGCT GAAAAAGGAG 
GGGACAGAAA TGTGGGGCGA TTGTCTGGAT GTGACGGAAG GTAGGGGTAA CCTGAGAAAG 
GAGAGGGGAA CAGCAGCAGG GTCAAGTAAC AGAGCCCGCC TCTGGGAAGC TCGAGGAGGC 
AGTGGGGCCG GAAGTCCTTG CGTCCCTGAG TTTCCTAGAG GAGGAGCTCA CAGCTCCAAG 
GCAGGCATGG GCCCTGAGTG CTGGCCAGGT GGAGATGCTG GCCATGGAGA AGCTGGGGTA 
TACTGGGGGG CAGGGGGATA TCCAGGACAG ACATCTGAGG GCAATGACAC CCAGAAATCA 
TCACTATCTG GAGACAGGAA ACTTCTGGGG TGCACGAGTC CTGAGGCCAA AGCTGAGGGC 
TCTTTCCAGA GCGCAGATGG TCATGGGATT GTGGGGAGGG TCTACAAACC TGGCCCTGAA 
GGCCCAGAAG ATCTTAGGAG TCAGAAAAGT GGCCTACAAG AGATTCAGGG AAGGAATGGT 
CAAGGCTCAG CTGGAGCCTT GGGCAGGACG GTGGAAGGTT CCAGAAGCCT CCAATTTCCT 
CAGAGCTGGA CAGTGGGACA GAGGGAAGCA GGGAAGCCAG GTGGAGCAGA GTATGAAGAC 
ATAGGTCCTC AGGATGACAC GTGGTCCAAT CTCAGAGAGA TGGGAGGCCA CTGTGGGTCT 
GGAGTGCTGG CGTCTGGTGG GAGGGAAGGG AGCGTGGGCG GTTCCCAAGT AGCTGCCCTG 
ATGATGTCTA GTCAGAGGGT AGATGCCAGA AACCACAGGC TAGTTACATC CCCAGGCCTG 
GGTGTCCAGG GTTCTGGCAG AACACTGGGA CACATGGAAG GATTAAGAGA CCCAGAGGCA 
GTGGGATCCG AAGAAGAGTT CTGGAATGGG TCAGGAAGGT CCCAAGGAAA AGGGTCCAGA 
GGTGGGATGG GCTTAGGAGG TCCAGAGTGG GCAGAGTCTA GGAATGAGGA GGGATGGGAG 
AATTCTGAAG GGATGGCTTC TAGGAATGGA GCCACTTGTC GTGATGGATC CAGGATGCAG 
GAAGGAACAG GATCTGGCCC TGATATTGGT TGCAAGGCCC CCTGTGGGGC ACCTGCGGAG 
ACAGAGTCTG GGAAGTGGGG TACTTACAGT CATGACTCAG GGGTGCCTGG GGGATTGTGG 
TCTGGAAGCA AAAGTCAGTG TAGTCCTGCA GGAAAGGTAT CTGGAAGAGA GGCCAGCCTC 
AGAAATGGCT CAGCTGGCCT TCATGGAATG CCTTCTAGTG AAGCTCAGCA GAGGGATGTA 
TTTCAAGGCC ACGGTCAGAC AGGCCACATT GGAGAGCACC ACTGTGCCCG TGGCTTAGGG 
AGTTCTGAGA CAGTGGGATC TGTGGGTGGA CATGGTAGGA AAGACTCTGG AACAGCGGGC 
AAGGTTGGAG AGGGATACAC AGAAGCAGAA CCAGGGCACT CTGGAGGGTT GAGTTCTTGG 
GGTCACACTG GAGATTATGA GGACTTCCGA GTCCTGGGGG CCTTTAGGGA AGGAGACTTT 
GGCAATGGCA CTGGGGTTCC ATTATCCATG GGACCAAGGT CTCTGGAGAG AGGGGACAAG 
GAAGGAGATG GAGAAAGGAT CAGTTTTCTT GGTGCCAGGA CCTCTACAGT TGGAACTGGG 
AATTGGGACA AAGCCAGGCA CCCTGGGGCA CCATCTCCTC ATGAGGCCGG TTCAGAGGGC 
CACTGGGCTC AAGTGTCTGG CAATGCATTA GGTTGTAGAG ATGGGTCTGG AATTCCAGAG 
CCTTGGAGTG CTGGCGACAA AACAGCTTAT GGAGAAGAAT CAAAGGGTCT TGGGCCTGAA 
AGAACAGGAC CAGATGGTGA GGTGGCATTT AGAGGTAGCT CTTCGAGCCT CAGAGGAATG 
GGACCAGCAA GTGAGGCAAG TTATAAAAGT GGAACTTGGG GCCCTGGGGC TGTGGGTTCA 
GGGTTTAAGG TAGATTGTAG GAATGATCTT GGGTGTTCTG GAAGTATGGG GTCAGAGAGT 
AAAGCAGGTT ATAAGAACAA AATTGAGAGT CATGGGGTCA GGGAGTTAGG GGACCTGACA 
GGCTATAAAA ATGATTTAGG ACCTTCTGAA CTTATTTCAT CAAGGAATGA GGCACGTGGT 
TTAGAACATT CTGGAGACAT TTTGTCATGG AATAAGGCAG GTTTAAGGGA TGGCTTGGGA 
GAAACAGGAA GAATGGAGTC CAAGAATGGG GTTGGTTATA GGGGAAGCTC AGTGGGTCCT 
GGGGAAATGG GGTCAGAGAG GAAAATGCAC CTTGCAGATG GTTCAGGGAG GCTAGGAGGA 
CCAGGGTCAC TGGCTGCGCC CAAAGAACAT GAATCTGTGG GTCTTGGGTC AGTTTATGAG 
GGAGCAGCAG AGATTGGCTC CAAATATAAT AGGGAGAAAG TCAGAGGAAT GGGGTTTAGA 
GATGCAACAG GGCCTGAGGT GGGCTCTGGG GTAGCTGGAA TGCTGGATAT TACAGGTGGC 
ATAGCACACG GGGACAGTGC TATGGGCACA GAGAAGCTGG GGACACCAGA TAGGCCTTAT 
ATCTTGTCAG GTGGGCAGGG TACCAAGAAC AGTCTTGGGG GCTGTGTGTC CTTGGGAATC 
CCTGACGCCT TGGGGGCTGT TGGCTCTGTG GGGAAACCAG GTATCAGAGA ATGGAAAGAT 
GATTCTGGGT TCCAAGGGTC TCTCAGGGAC AGAGGGACTC CGAGTGAGGA GATCAGATCT 
GTAGACCAAG CAGGAGCTAT AGGGACATCT CGGCTTCTTG ATAGTAGGGG GGCAGTGGAG 
GATGGGAGTG GGTCAGAAAA TGACTTTGAC TTTAATAAAT CAGGCTTGGA GACAACAGGA 
AGATGTAGAG TTGCTGACCA GCAGGGAGTG ACACCACAGG GATGTGGAGG CTCATTGCTC 
AATGGCAGGA GGAAGGGAAC AAGTTCTGGA AGCTTGTCAG GGGTAGGGCA GGAGGTAGAC 
AGAAGTTCTA CACCTGGGAG GGAGACCAAG GTAATGTCAG GACCTGGCAG TGGGCGAGCC 
ACAAGCCACA CTCGGGCTGC AGACTGGGAA GACGGGGTTC AATGCAGCTT CGGGGCTTCT 
AGCTCACTGC CTGATACTGA TGCCATTTTC AGAGAAACCC ATGCGGGACA AGGAGCCTTC 
AAGGGCAGGG GCTGTGAAAC CGGCCAAGGG GCTGCTGGAG AGTGTCAAGG ACCACGCTCC 
TTGGGGAGCA GAGGCTCAGA GTTTGTAGGG GGCAGGGCTG GATTTACAGG GAGCTCAAGT 
GTTCCAGGCA GAAGGGATTC CACTATCTAC GGAGATGCTG TCACATCAAA GCCACAGGAA 
CCCCAGAATG AATTATATAG TCCTTTAGGC AGAAGGGACT TCAAAAGTGT ATCGGGAGGA 
ATCCAGGAGC CAGGTTTTCA ACGAGGAACA GGACAGGGAG AAGGAAAAGA ATCCTTCCAG 
GAGTCAGGGT CCTGGGAGGC TGAGCTTGAC GAGGTCCAAG GATCCAGGGC CTCAGCAAAG 
TTTGAAAACC AGGGAGGGAA AGACCCTGGC TGGTCAGGCC AGAAGCCTGG TCCCTGTGGA 
AGCAGGCATC AGGTGCAGTC TGGGACTGAA GTTGGATCAG CAAAGAGAGC AACCCCACAG 
GGGGCCGGAG GGCTGGAGCC TTGGGCTGGC AGTGAGGATA GAGGGTCTCT GAGAGAACCT 
TGGAGTGAAG ACAGGAGGCA AGGCCCCCAC AGGCACCTTG GCAGCAGGAG AGATACCCAA 
GAGGGCAGGT CAGATGTCTG TGGCCAGGCA CAAGATGCTA CCCAGAGCCC CAGATCCAGA 
TACCAGCCTG GCACTGGCCG CTCTTCTTCA GAGGCCCGAG GCTCCATGGA CCACTTCTCT 
CAGGGTCTGA CTGATACAGA GGTGCAACTG GGGGAAGCCG CCATAGTCTC CTGTACTCTT 
GCCAGTGACC TGGGACCTGG CACGTGGTTC AAGGATGGAG TCAAGCTCAC TGCCCAAGAT 
GGAGTCATCT TTGAGCAAGA TGGGCTCACG CACAGACTGA TTCTCACCCA CGTGGAGGGG 
ACCCAGGCTG GGAAATACAC TTTTGTAGCC GGCTGCCAGC ACAGCGAGGC CAGCCTGACC 
GTGCAGGATC CCCCCACCAT TGCTCCAGAT GTGACAGAGA CACTGAGAGA GCCACTGGTG 
TTCAAGGCTG GGAAGCCAGT CACCGTGAAA ATCCCTTTCC AGAGCCGCCT CCCTGTTCAG 
GCTGCCTGGA GGAAAGATGG GAACGAGGTG GTGGGCGGCG ACCACAAGGG CATCCAACTG 
GCTCTGGCAG ATGGCTACAC ATGGCTGTGC CTCCCCAGCG TGTGCAGGAA GGACAGTGGC 
CGGTACAGCG TGACGCTGAG GAGCGAGGGA GGCTGTGTGC AGGCTGAGTT CACCCTGCAA 
GTTATAGACA AGCCTCAGCC CCCACAAGGA CCCCTGGAGG TTCAGGATTG CCACAGAGCA 
GGTGTCTGCC TCCGCTGGCT ACCCCCGAGG GACAACGGGG GCCAGGTCAT ACAACACTAT 
GTGGTGGAGA GGCGACAGGC TGGAAGGAGC ACTTGGTTGA AGGTGGGCGA GCCCCCATCA 
GACAGTACCA GCTTCACCGA CACCAGCGTG GAGCAGGGAA AGAAATATGC CTTCCGTGTG 
CGGGCTGTGA CCTCAGTGGG AGCCGGAGAT GCCCTGGAGT CAGAGGAGGT GTTGGTGGCT 
CCTGAGGCTC TCCCGGGGCC CCCTTCTGCC CCAGCCATCC TGTCAGCCTC CAGTCAGAGC 
ATCACTCTCA CATGGGGGGC ACCACAGGGC CCTGGCAGTA ACCACATCCT GGGCTACCTG 
ATCGAGAAGC ACAAGAAGGG GAGCAACACC TGGATGGCTG TGAATGAGCA GCCCGTGTCT 
GAAAGAAGGT ACACTGTGGT GGGTCTGCGA CAGGGCTGCC AGTATGAGTT CCGGGTCACG 
GCCGTGACTC TGTCGGGCCC TGGAGATCCT GGGCCTCCAT CGGATGCTGT CTTTGCTAGA 
GACCCTATGA GACCCCCAGG GCCTGTGCGG GATCTACAGG TTACAGACAC ATCAAACACT 
AGCATCACCG TGAGCTGGAT GGTGCCGGAT GCCCGGGATG CTGATGAAGC CCAGGGATAT 
ATTGTGGAGT TGTGTGGTTC AGACAGCCAT CAATGGAGCC CATGTCACGT GGGAACTGTG 
CCGGGCACCA CCTTCACAGC CAAGGGGCTT CGGCCGCAAG AAGGCTACTT TGTGCGGGTG 
ACAGCAGTTA ATGATGGGGG CCGGAGCCAG GCCACTTCCC TGGACACATT GGTTCATGCC 
ATGCCGGCCA CTGTCTGTCC CAGGTTCCTC ATGGACTCCG GCACAAAGGA TACCCTGATG 
GTCAGGGTTG GGGACAGTAT TCGCGTTCCT CTCTCCTTTG AAGCTGCCCC CATGCCTGAG 
GTGACCTGGC TGAAGGACGG CTTGCCCTTG CCCAAAAGAA GTGTGACCAC TGTGAAGGCT 
GGCCTCACGC AGCTTCTGAT CCCTGCAGCT AGCCTCTCGG ACTGTGGCCG ATACACCGTG 
ATGCTGAGGA ACCTACAGGG GAAGGAAGCT ACCCACAGCT TCTTCATCAA TGTAGCAGCA 
TGCCCACAGG CACCAGGTTC TATCTACCTA CAGGAGAATG TGCCTGGGAC AGTGACGGTC 
CAGTGGGAAC CCTCTCCGGA TGAGGCTCAG GGCATCCCCT TGCATTACAC AGTGCTGATG 
CGGTCCTCAT CTCACCGATC CTGGCACGAG GTGGCCGACC ATGTCCGTAC CAACCGCTTT 
ACCCTCCTAG GGGTCCTCCC TGGCCATGAG TACCACTTTC GGGTGCTGGC CAAGAATGAG 
CTGGGGGTCA GCAAACCTTC AGACACCAGC CAACCCTGGT GTATCCCCAG GCAGAGAGAC 
AGGTTCACAG TGAAGTCTCC GACATACCAG GATCCTGACC TGAGCCAGAA GCCCCGCTTC 
CTGGTGGGTC TGCGGGCTCA CCTTCTGCCC CAGGGCTGTG AGTGCCGCAT GACGTGTGCC 
GTGCAGGGCT CACCCCAGCC TCATGTCACC TGGTTCAAGA ATGACCAAAG CTTGGACAGA 
AACCCTGCGG TGTACAGTAC TGACTTGCTG GGTGTGTGCT CCCTCGTCAT CCCCAGCGTG 
TCCCTTAAGG ACAGTGGTGA ATACAAGGCT GTGGCCAAGA ACCCACTGGG ACAAGCTGTC 
AGCACAGCCA CCCTCATTGT CACAGAATAC GACTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

IGFN1_v1 is required for myoblast fusion and differentiation.
PloS one12(6)e0180217(2017)
Li X,Baker J,Cracknell T,Haynes AR,Blanco G

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Neuron83(2)309-323(2014 Jul)
Thompson CL,Ng L,Menon V,Martinez S,Lee CK,Glattfelder K,Sunkin SM,Henry A,Lau C,Dang C,Garcia-Lopez R,Martinez-Ferre A,Pombero A,Rubenstein JLR,Wakeman WB,Hohmann J,Dee N,Sodt AJ,Young R,Smith K,Nguyen TN,Kidney J,Kuan L,Jeromin A,Kaykas A,Miller J,Page D,Orta G,Bernard A,Riley Z,Smith S,Wohnoutka P,Hawrylycz MJ,Puelles L,Jones AR

Identification of a Z-band associated protein complex involving KY, FLNC and IGFN1.
Experimental cell research316(11)1856-70(2010 Jul)
Baker J,Riley G,Romero MR,Haynes AR,Hilton H,Simon M,Hancock J,Tateossian H,Ripoll VM,Blanco G

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Igfn1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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