Eno4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Eno4
Entrez Gene ID	226265
Full Name	enolase 4
Synonyms	6430537H07Rik
General protein information	Preferred Names enolase 4 Names enolase 4 2-phospho-D-glycerate hydro-lyase enolase-like protein C10orf134 homolog enolase-like protein ENO4
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 19 Map Location: 19\|19 D2

mRNA and Protein(s)

mRNA	Protein	Name
XM_006527056.3	XP_006527119.1	enolase 4 isoform X3
XM_006527055.4	XP_006527118.1	enolase 4 isoform X2
XM_006527056.4	XP_006527119.1	enolase 4 isoform X3
NM_178689.4	NP_848804.2	enolase 4
XM_030250922.1	XP_030106782.1	enolase 4 isoform X4
XM_006527054.3	XP_006527117.1	enolase 4 isoform X1
XM_006527055.3	XP_006527118.1	enolase 4 isoform X2
XM_006527054.4	XP_006527117.1	enolase 4 isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Eno4	NP_848804.2
Gallus gallus (chicken)	ENO4	XP_421780.4
Xenopus tropicalis (tropical clawed frog)	eno4	NP_001119992.1
	ENO4	XP_001095040.2
Canis lupus familiaris (dog)	ENO4	XP_535025.3
Rattus norvegicus (Norway rat)	Eno4	NP_001127977.1
Danio rerio (zebrafish)	eno4	NP_001071015.1
Pan troglodytes (chimpanzee)	ENO4	XP_001145271.3
Homo sapiens (human)	ENO4	NP_001229628.1
Bos taurus (cattle)	ENO4	NP_001192302.1

Gene Ontology
Bibliography

Related articles in PubMed

Disruption of a spermatogenic cell-specific mouse enolase 4 (eno4) gene causes sperm structural defects and male infertility.
Nakamura N, Dai Q, Williams J, Goulding EH, Willis WD, Brown PR, Eddy EM
Biology of reproduction88(4)90(2013 Apr)

Integrated microarray and ChIP analysis identifies multiple Foxa2 dependent target genes in the notochord.
Tamplin OJ, Cox BJ, Rossant J
Developmental biology360(2)415-25(2011 Dec)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ENO4 is required for normal assembly of the fibrous sheath and provides most of the enolase activity in sperm.
Title: Disruption of a spermatogenic cell-specific mouse enolase 4 (eno4) gene causes sperm structural defects and male infertility.

The following Eno4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Eno4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu07568	NM_178689.4 Latest version!	Mus musculus enolase 4 (Eno4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu87571	XM_030250922.1 Latest version!	Mus musculus enolase 4 (Eno4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu55538	XM_006527054.4 Latest version!	Mus musculus enolase 4 (Eno4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu55539	XM_006527055.4 Latest version!	Mus musculus enolase 4 (Eno4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu55540	XM_006527056.4 Latest version!	Mus musculus enolase 4 (Eno4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu55540	XM_006527056.3	Mus musculus enolase 4 (Eno4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu55539	XM_006527055.3	Mus musculus enolase 4 (Eno4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu55538	XM_006527054.3	Mus musculus enolase 4 (Eno4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Eno4 ( NM_178689.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_848804.2 Mus musculus enolase 4 (Eno4), mRNA.

Price & Availability↑

CloneID	OMu07568
Clone ID Related Accession (Same CDS sequence)	NM_178689.4
Accession Version	NM_178689.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1857bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Mus musculus(house mouse)
Product	enolase 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY000779.1, BB614863.1, AK032395.1 and BQ174362.1. On Nov 17, 2007 this sequence version replaced NM_178689.3. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849384, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGAGATG AAGACGGCGG CCGCAGAGGC GGGATCACTA GGGACCTGCA GAAGCTGAAG 
CAGCAGGCGA TGGCGTACTA CCAGGAGAAC GACGTCCCGC GCAAGCTGGA AGATCTGCTC 
AACTCCACCT TCTACCTCCA GCCTGCGGAT GTCTACGGGC ACCTGGCAAA CTACTTTTCT 
AAACTTGCGA AGCCTCCCTC CATATGCAAA ATAGTAGGGA AAACCATACT GGATGGCCTG 
GGACTTCCTA CTCTACAGGT GGAAATCTCC TGCACCATTC AAAATTTCCC CAAGTACATC 
TGTGCCGTGG CGATCCCTAC GCACTTTGAA GTCGTGGAGA ACGCTTTGCC AGAGGCGTTG 
GATGCAGAGG ACTCAGAAAG GGCCCAGGCC GTGAACACAG CGGTGCAGTG GATCAATCAG 
TCCATCACAG AGGAGCTGTG GGGTCTGGTC CCCTCCAACC AGGCAGAGGT GGACCACAGG 
CTCAGGACCT TCTTTGAACA TAAAGTACAA GAAGATAAAG AGAGAAAAGA ATTGGAAAAG 
AGCCAGGAGG AGCTGGTTCC CGCACCTCCA CCAGTAACGC TGCCGCCGCC GCCGCCGCCG 
CCTCCCCCAC CTCCCTCCAA AAAGAAAGGG CAGAAGGCAG GACGGAGGGA TACGCTTTTG 
GAGAAACCTG TTTCACCCCC GGAGCCCCCT GAACCAGTCC TCCATGGTAG CATGGCCATA 
GGCGCTGTGT CCCTGGCAGT GGCCAAAGCC AGCGCAACCT TGGCCAGCGA TCCTCTCTAC 
TTAACCCTGG CGTCACTGAA GCATGATCAG GAGCAGCCGT CAACATTCTC TATGCCTTTG 
CTGATGGGAT CTGTACTGAG CTGTGGGAAG TCGTCACCTG GGAAGCTACA TTTAATGAAA 
GAAGTGATTT GTATTCCCAG TCCTGGATTA ACAGCCAAAC AAAGTGTCGA GTTGCTTCTG 
GAAATTCAGA AACAAGTTAA CAGAGCGATG GAGACGCTTC CACCTCCAAA ACAAGAGACC 
AAAAAAGGGC ATAATGGGAG CAAAAGAGCT CAGCCACCGA TCACTGGCAA GGTATCTCAC 
CTTGGCTGTT TAACCATCAA CTACGACGCC ATAGAGCAGC CACTGCTGCT GCTTCAGGGG 
ATCTGCTCAA ACCTGGGGTT GGAACTGGGA GTGAACTTCC ATCTAGCCAT CAACTGTGCT 
GGGCATGAGC TGATGGACTA CAGTAAAGGG AAGTACGAAG TGATGGTGGG CACCCACAAA 
AGCGCAGCTG AGATGGTGGA GCTCTACGTG GACCTGATCA ACAAGTATCC TTCCATAATC 
GCCTTAATTG ATCCTTTCAG GAAGGAGGAC GCCGAGCAGT GGGACAGCCT CTATGCTGCT 
CTGGCTTCCC GGTGCTACCT AATTGCAGGC GCCGCTTCCG GGAGTGTCTC TAAACTCCTA 
GAGTGCAGAA ATATAAGCAC CCTGAAATCC CACGGACTGA TCATAAAGCA CACAAACCAA 
ACCACAATGT CTGACTTGGT GGAAATAACC CATCTTATCA ACGGTAAGAA GCTCCTGGCC 
GTCTTTGGAA GCACAGACTC GGAGTCCTCT GATGACAGCC TTGTCGATTT GGCTGTTGGA 
TTCGGTGCCC GGTTTATCAA GTTGGGGGGT CTTTCTCGGG GTGAACGGAT GACCAAATAC 
AACCGCCTTC TTGCTATAGA GGAAGAACTC ATCCAGAGGG GAGTATGGGG TTTCAGTGAA 
GAACACAATT TTTCTTTCTT TCAAGAGGAT GCTACTGCCA CAATGGCTGA GGAAACTCTT 
GGGCTCCTGG ACTCCATCTT CCCCACAGAG GTGATAGAGG AATCGGCTAA AACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_848804.2
CDS	33..1889
Translation	MGDEDGGRRGGITRDLQKLKQQAMAYYQENDVPRKLEDLLNSTFYLQPADVYGHLANYFSKLAKPPSICKIVGKTILDGLGLPTLQVEISCTIQNFPKYICAVAIPTHFEVVENALPEALDAEDSERAQAVNTAVQWINQSITEELWGLVPSNQAEVDHRLRTFFEHKVQEDKERKELEKSQEELVPAPPPVTLPPPPPPPPPPPSKKKGQKAGRRDTLLEKPVSPPEPPEPVLHGSMAIGAVSLAVAKASATLASDPLYLTLASLKHDQEQPSTFSMPLLMGSVLSCGKSSPGKLHLMKEVICIPSPGLTAKQSVELLLEIQKQVNRAMETLPPPKQETKKGHNGSKRAQPPITGKVSHLGCLTINYDAIEQPLLLLQGICSNLGLELGVNFHLAINCAGHELMDYSKGKYEVMVGTHKSAAEMVELYVDLINKYPSIIALIDPFRKEDAEQWDSLYAALASRCYLIAGAASGSVSKLLECRNISTLKSHGLIIKHTNQTTMSDLVEITHLINGKKLLAVFGSTDSESSDDSLVDLAVGFGARFIKLGGLSRGERMTKYNRLLAIEEELIQRGVWGFSEEHNFSFFQEDATATMAEETLGLLDSIFPTEVIEESAKT

Target ORF information:

RefSeq Version	NM_178689.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus enolase 4 (Eno4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178689.4

ORF Insert Sequence:

ATGGGAGATG AAGACGGCGG CCGCAGAGGC GGGATCACTA GGGACCTGCA GAAGCTGAAG 
CAGCAGGCGA TGGCGTACTA CCAGGAGAAC GACGTCCCGC GCAAGCTGGA AGATCTGCTC 
AACTCCACCT TCTACCTCCA GCCTGCGGAT GTCTACGGGC ACCTGGCAAA CTACTTTTCT 
AAACTTGCGA AGCCTCCCTC CATATGCAAA ATAGTAGGGA AAACCATACT GGATGGCCTG 
GGACTTCCTA CTCTACAGGT GGAAATCTCC TGCACCATTC AAAATTTCCC CAAGTACATC 
TGTGCCGTGG CGATCCCTAC GCACTTTGAA GTCGTGGAGA ACGCTTTGCC AGAGGCGTTG 
GATGCAGAGG ACTCAGAAAG GGCCCAGGCC GTGAACACAG CGGTGCAGTG GATCAATCAG 
TCCATCACAG AGGAGCTGTG GGGTCTGGTC CCCTCCAACC AGGCAGAGGT GGACCACAGG 
CTCAGGACCT TCTTTGAACA TAAAGTACAA GAAGATAAAG AGAGAAAAGA ATTGGAAAAG 
AGCCAGGAGG AGCTGGTTCC CGCACCTCCA CCAGTAACGC TGCCGCCGCC GCCGCCGCCG 
CCTCCCCCAC CTCCCTCCAA AAAGAAAGGG CAGAAGGCAG GACGGAGGGA TACGCTTTTG 
GAGAAACCTG TTTCACCCCC GGAGCCCCCT GAACCAGTCC TCCATGGTAG CATGGCCATA 
GGCGCTGTGT CCCTGGCAGT GGCCAAAGCC AGCGCAACCT TGGCCAGCGA TCCTCTCTAC 
TTAACCCTGG CGTCACTGAA GCATGATCAG GAGCAGCCGT CAACATTCTC TATGCCTTTG 
CTGATGGGAT CTGTACTGAG CTGTGGGAAG TCGTCACCTG GGAAGCTACA TTTAATGAAA 
GAAGTGATTT GTATTCCCAG TCCTGGATTA ACAGCCAAAC AAAGTGTCGA GTTGCTTCTG 
GAAATTCAGA AACAAGTTAA CAGAGCGATG GAGACGCTTC CACCTCCAAA ACAAGAGACC 
AAAAAAGGGC ATAATGGGAG CAAAAGAGCT CAGCCACCGA TCACTGGCAA GGTATCTCAC 
CTTGGCTGTT TAACCATCAA CTACGACGCC ATAGAGCAGC CACTGCTGCT GCTTCAGGGG 
ATCTGCTCAA ACCTGGGGTT GGAACTGGGA GTGAACTTCC ATCTAGCCAT CAACTGTGCT 
GGGCATGAGC TGATGGACTA CAGTAAAGGG AAGTACGAAG TGATGGTGGG CACCCACAAA 
AGCGCAGCTG AGATGGTGGA GCTCTACGTG GACCTGATCA ACAAGTATCC TTCCATAATC 
GCCTTAATTG ATCCTTTCAG GAAGGAGGAC GCCGAGCAGT GGGACAGCCT CTATGCTGCT 
CTGGCTTCCC GGTGCTACCT AATTGCAGGC GCCGCTTCCG GGAGTGTCTC TAAACTCCTA 
GAGTGCAGAA ATATAAGCAC CCTGAAATCC CACGGACTGA TCATAAAGCA CACAAACCAA 
ACCACAATGT CTGACTTGGT GGAAATAACC CATCTTATCA ACGGTAAGAA GCTCCTGGCC 
GTCTTTGGAA GCACAGACTC GGAGTCCTCT GATGACAGCC TTGTCGATTT GGCTGTTGGA 
TTCGGTGCCC GGTTTATCAA GTTGGGGGGT CTTTCTCGGG GTGAACGGAT GACCAAATAC 
AACCGCCTTC TTGCTATAGA GGAAGAACTC ATCCAGAGGG GAGTATGGGG TTTCAGTGAA 
GAACACAATT TTTCTTTCTT TCAAGAGGAT GCTACTGCCA CAATGGCTGA GGAAACTCTT 
GGGCTCCTGG ACTCCATCTT CCCCACAGAG GTGATAGAGG AATCGGCTAA AACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eno4 ( XM_006527054.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527117.1 Mus musculus enolase 4 (Eno4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu55538
Clone ID Related Accession (Same CDS sequence)	XM_006527054.3 , XM_006527054.4
Accession Version	XM_006527054.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	enolase 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006527054.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGATG AAGACGGCGG CCGCAGAGGC GGGATCACTA GGGACCTGCA GAAGCTGAAG 
CAGCAGGCGA TGGCGTACTA CCAGGAGAAC GACGTCCCGC GCAAGCTGGA AGATCTGCTC 
AACTCCACCT TCTACCTCCA GCCTGCGGAT GTCTACGGGC ACCTGGCAAA CTACTTTTCT 
AAACTTGCGA AGCCTCCCTC CATATGCAAA ATAGTAGGGA AAACCATACT GGATGGCCTG 
GGACTTCCTA CTCTACAGGT GGAAATCTCC TGCACCATTC AAAATTTCCC CAAGTACATC 
TGTGCCGTGG CGATCCCTAC GCACTTTGAA GTCGTGGAGA ACGCTTTGCC AGAGGCGTTG 
GATGCAGAGG ACTCAGAAAG GGCCCAGGCC GTGAACACAG CGGTGCAGTG GATCAATCAG 
TCCATCACAG AGGAGCTGTG GGGTCTGGTC CCCTCCAACC AGGCAGAGGT GGACCACAGG 
CTCAGGACCT TCTTTGAACA TAAAGTACAA GAAGATAAAG AGAGAAAAGA ATTGGAAAAG 
AGCCAGGAGG AGCTGGTTCC CGCACCTCCA CCAGTAACGC TGCCGCCGCC GCCGCCGCCG 
CCTCCCCCAC CTCCCTCCAA AAAGAAAGGG CAGAAGGCAG GACGGAGGGA TACGCTTTTG 
GAGAAACCTG TTTCACCCCC GGAGCCCCCT GAACCAGTCC TCCATGGTAG CATGGCCATA 
GGCGCTGTGT CCCTGGCAGT GGCCAAAGCC AGCGCAACCT TGGCCAGCGA TCCTCTCTAC 
TTAACCCTGG CGTCACTGAA GCATGATCAG GAGCAGCCGT CAACATTCTC TATGCCTTTG 
CTGATGGGAT CTGTACTGAG CTGTGGGAAG TCGTCACCTG GGAAGCTACA TTTAATGAAA 
GAAGTGATTT GTATTCCCAG TCCTGGATTA ACAGCCAAAC AAAGTGTCGA GTTGCTTCTG 
GAAATTCAGA AACAAGTTAA CAGAGCGATG GAGACGCTTC CACCTCCAAA ACAAGAGACC 
AAAAAAGGGC ATAATGGGAG CAAAAGAGCT CAGCCACCGA TCACTGGCAA GGTATCTCAC 
CTTGGCTGTT TAACCATCAA CTACGACGCC ATAGAGCAGC CACTGCTGCT GCTTCAGGGG 
ATCTGCTCAA ACCTGGGGTT GGAACTGGGA GTGAACTTCC ATCTAGCCAT CAACTGTGCT 
GGGCATGAGC TGATGGACTA CAGTAAAGGG AAGTACGAAG TGATGGTGGG CACCCACAAA 
AGCGCAGCTG AGATGGTGGA GCTCTACGTG GACCTGATCA ACAAGTATCC TTCCATAATC 
GCCTTAATTG ATCCTTTCAG GAAGGAGGAC GCCGAGCAGT GGGACAGCCT CTATGCTGCT 
CTGGCTTCCC GGTGCTACCT AATTGCAGGC GCCGCTTCCG GGAGTGTCTC TAAACTCCTA 
GAGTGCAGAA ATATAAGCAC CCTGAAATCC CACGGACTGA TCATAAAGCA CACAAACCAA 
ACCACAATGT CTGACTTGGT GGAAATAACC CATCTTATCA ACGGTAAGAA GCTCCTGGCC 
GTCTTTGGAA GCACAGACTC GGAGTCCTCT GATGACAGCC TTGTCGATTT GGCTGTTGGA 
TTCGGTGCCC GGTTTATCAA GTTGGGGGGT CTTTCTCGGG GTGAACGGAT GACCAAATAC 
AACCGCCTTC TTGCTATAGA GGAAGAACTC ATCCAGAGGG GAGTATGGGA GGATGCTACT 
GCCACAATGG CTGAGGAAAC TCTTGGGCTC CTGGACTCCA TCTTCCCCAC AGAGGTGATA 
GAGGAATCGG CTAAAACATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006527117.1
CDS	8274..10094
Translation	MGDEDGGRRGGITRDLQKLKQQAMAYYQENDVPRKLEDLLNSTFYLQPADVYGHLANYFSKLAKPPSICKIVGKTILDGLGLPTLQVEISCTIQNFPKYICAVAIPTHFEVVENALPEALDAEDSERAQAVNTAVQWINQSITEELWGLVPSNQAEVDHRLRTFFEHKVQEDKERKELEKSQEELVPAPPPVTLPPPPPPPPPPPSKKKGQKAGRRDTLLEKPVSPPEPPEPVLHGSMAIGAVSLAVAKASATLASDPLYLTLASLKHDQEQPSTFSMPLLMGSVLSCGKSSPGKLHLMKEVICIPSPGLTAKQSVELLLEIQKQVNRAMETLPPPKQETKKGHNGSKRAQPPITGKVSHLGCLTINYDAIEQPLLLLQGICSNLGLELGVNFHLAINCAGHELMDYSKGKYEVMVGTHKSAAEMVELYVDLINKYPSIIALIDPFRKEDAEQWDSLYAALASRCYLIAGAASGSVSKLLECRNISTLKSHGLIIKHTNQTTMSDLVEITHLINGKKLLAVFGSTDSESSDDSLVDLAVGFGARFIKLGGLSRGERMTKYNRLLAIEEELIQRGVWEDATATMAEETLGLLDSIFPTEVIEESAKT

Target ORF information:

RefSeq Version	XM_006527054.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus enolase 4 (Eno4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527054.3

ORF Insert Sequence:

ATGGGAGATG AAGACGGCGG CCGCAGAGGC GGGATCACTA GGGACCTGCA GAAGCTGAAG 
CAGCAGGCGA TGGCGTACTA CCAGGAGAAC GACGTCCCGC GCAAGCTGGA AGATCTGCTC 
AACTCCACCT TCTACCTCCA GCCTGCGGAT GTCTACGGGC ACCTGGCAAA CTACTTTTCT 
AAACTTGCGA AGCCTCCCTC CATATGCAAA ATAGTAGGGA AAACCATACT GGATGGCCTG 
GGACTTCCTA CTCTACAGGT GGAAATCTCC TGCACCATTC AAAATTTCCC CAAGTACATC 
TGTGCCGTGG CGATCCCTAC GCACTTTGAA GTCGTGGAGA ACGCTTTGCC AGAGGCGTTG 
GATGCAGAGG ACTCAGAAAG GGCCCAGGCC GTGAACACAG CGGTGCAGTG GATCAATCAG 
TCCATCACAG AGGAGCTGTG GGGTCTGGTC CCCTCCAACC AGGCAGAGGT GGACCACAGG 
CTCAGGACCT TCTTTGAACA TAAAGTACAA GAAGATAAAG AGAGAAAAGA ATTGGAAAAG 
AGCCAGGAGG AGCTGGTTCC CGCACCTCCA CCAGTAACGC TGCCGCCGCC GCCGCCGCCG 
CCTCCCCCAC CTCCCTCCAA AAAGAAAGGG CAGAAGGCAG GACGGAGGGA TACGCTTTTG 
GAGAAACCTG TTTCACCCCC GGAGCCCCCT GAACCAGTCC TCCATGGTAG CATGGCCATA 
GGCGCTGTGT CCCTGGCAGT GGCCAAAGCC AGCGCAACCT TGGCCAGCGA TCCTCTCTAC 
TTAACCCTGG CGTCACTGAA GCATGATCAG GAGCAGCCGT CAACATTCTC TATGCCTTTG 
CTGATGGGAT CTGTACTGAG CTGTGGGAAG TCGTCACCTG GGAAGCTACA TTTAATGAAA 
GAAGTGATTT GTATTCCCAG TCCTGGATTA ACAGCCAAAC AAAGTGTCGA GTTGCTTCTG 
GAAATTCAGA AACAAGTTAA CAGAGCGATG GAGACGCTTC CACCTCCAAA ACAAGAGACC 
AAAAAAGGGC ATAATGGGAG CAAAAGAGCT CAGCCACCGA TCACTGGCAA GGTATCTCAC 
CTTGGCTGTT TAACCATCAA CTACGACGCC ATAGAGCAGC CACTGCTGCT GCTTCAGGGG 
ATCTGCTCAA ACCTGGGGTT GGAACTGGGA GTGAACTTCC ATCTAGCCAT CAACTGTGCT 
GGGCATGAGC TGATGGACTA CAGTAAAGGG AAGTACGAAG TGATGGTGGG CACCCACAAA 
AGCGCAGCTG AGATGGTGGA GCTCTACGTG GACCTGATCA ACAAGTATCC TTCCATAATC 
GCCTTAATTG ATCCTTTCAG GAAGGAGGAC GCCGAGCAGT GGGACAGCCT CTATGCTGCT 
CTGGCTTCCC GGTGCTACCT AATTGCAGGC GCCGCTTCCG GGAGTGTCTC TAAACTCCTA 
GAGTGCAGAA ATATAAGCAC CCTGAAATCC CACGGACTGA TCATAAAGCA CACAAACCAA 
ACCACAATGT CTGACTTGGT GGAAATAACC CATCTTATCA ACGGTAAGAA GCTCCTGGCC 
GTCTTTGGAA GCACAGACTC GGAGTCCTCT GATGACAGCC TTGTCGATTT GGCTGTTGGA 
TTCGGTGCCC GGTTTATCAA GTTGGGGGGT CTTTCTCGGG GTGAACGGAT GACCAAATAC 
AACCGCCTTC TTGCTATAGA GGAAGAACTC ATCCAGAGGG GAGTATGGGA GGATGCTACT 
GCCACAATGG CTGAGGAAAC TCTTGGGCTC CTGGACTCCA TCTTCCCCAC AGAGGTGATA 
GAGGAATCGG CTAAAACATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eno4 ( XM_006527055.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527118.1 Mus musculus enolase 4 (Eno4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu55539
Clone ID Related Accession (Same CDS sequence)	XM_006527055.4 , XM_006527055.3
Accession Version	XM_006527055.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1716bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	enolase 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006527055.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGATG AAGACGGCGG CCGCAGAGGC GGGATCACTA GGGACCTGCA GAAGCTGAAG 
CAGCAGGCGA TGGCGTACTA CCAGGAGAAC GACGTCCCGC GCAAGCTGGA AGATCTGCTC 
AACTCCACCT TCTACCTCCA GCCTGCGGAT GTCTACGGGC ACCTGGCAAA CTACTTTTCT 
AAACTTGCGA AGCCTCCCTC CATATGCAAA ATAGTAGGGA AAACCATACT GGATGGCCTG 
GGACTTCCTA CTCTACAGGT GGAAATCTCC TGCACCATTC AAAATTTCCC CAAGTACATC 
TGTGCCGTGG CGATCCCTAC GCACTTTGAA GTCGTGGAGA ACGCTTTGCC AGAGGCGTTG 
GATGCAGAGG ACTCAGAAAG GGCCCAGGCC GTGAACACAG CGGTGCAGTG GATCAATCAG 
TCCATCACAG AGGAGCTGTG GGGTCTGGTC CCCTCCAACC AGGCAGAGGT GGACCACAGG 
CTCAGGACCT TCTTTGAACA TAAAGTACAA GAAGATAAAG AGAGAAAAGA ATTGGAAAAG 
AGCCAGGAGG AGCTGGTTCC CGCACCTCCA CCAGTAACGC TGCCGCCGCC GCCGCCGCCG 
CCTCCCCCAC CTCCCTCCAA AAAGAAAGGG CAGAAGGCAG GACGGAGGGA TACGCTTTTG 
GAGAAACCTG TTTCACCCCC GGAGCCCCCT GAACCAGTCC TCCATGGTAG CATGGCCATA 
GGCGCTGTGT CCCTGGCAGT GGCCAAAGCC AGCGCAACCT TGGCCAGCGA TCCTCTCTAC 
TTAACCCTGG CGTCACTGAA GCATGATCAG GAGCAGCCGT CAACATTCTC TATGCCTTTG 
CTGATGGGAT CTGTACTGAG CTGTGGGAAG TCGTCACCTG GGAAGCTACA TTTAATGAAA 
GAAGTGATTT GTATTCCCAG TCCTGGATTA ACAGCCAAAC AAAGTGTCGA GTTGCTTCTG 
GAAATTCAGA AACAAGTTAA CAGAGCGATG GAGACGCTTC CACCTCCAAA ACAAGAGACC 
AAAAAAGGGC ATAATGGGAG CAAAAGAGCT CAGCCACCGA TCACTGGCAA GGTATCTCAC 
CTTGGCTGTT TAACCATCAA CTACGACGCC ATAGAGCAGC CACTGCTGCT GCTTCAGGGG 
ATCTGCTCAA ACCTGGGGTT GGAACTGGGA GTGAACTTCC ATCTAGCCAT CAACTGTGCT 
GGGCATGAGC TGATGGACTA CAGTAAAGGG AAGTACGAAG TGATGGTGGG CACCCACAAA 
AGCGCAGCTG AGATGGTGGA GCTCTACGTG GACCTGATCA ACAAGTATCC TTCCATAATC 
GCCTTAATTG ATCCTTTCAG GAAGGAGGAC GCCGAGCAGT GGGACAGCCT CTATGCTGCT 
CTGGCTTCCC GGTGCTACCT AATTGCAGGC GCCGCTTCCG GGAGTGTCTC TAAACTCCTA 
GAGTGCAGAA ATATAAGCAC CCTGAAATCC CACGGACTGA TCATAAAGCA CACAAACCAA 
ACCACAATGT CTGACTTGGT GGAAATAACC CATCTTATCA ACGGTAAGAA GCTCCTGGCC 
GTCTTTGGAA GCACAGACTC GGAGTCCTCT GATGACAGCC TTGTCGATTT GGTTTCAGTG 
AAGAACACAA TTTTTCTTTC TTTCAAGAGG ATGCTACTGC CACAATGGCT GAGGAAACTC 
TTGGGCTCCT GGACTCCATC TTCCCCACAG AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006527118.1
CDS	8274..9989
Translation	MGDEDGGRRGGITRDLQKLKQQAMAYYQENDVPRKLEDLLNSTFYLQPADVYGHLANYFSKLAKPPSICKIVGKTILDGLGLPTLQVEISCTIQNFPKYICAVAIPTHFEVVENALPEALDAEDSERAQAVNTAVQWINQSITEELWGLVPSNQAEVDHRLRTFFEHKVQEDKERKELEKSQEELVPAPPPVTLPPPPPPPPPPPSKKKGQKAGRRDTLLEKPVSPPEPPEPVLHGSMAIGAVSLAVAKASATLASDPLYLTLASLKHDQEQPSTFSMPLLMGSVLSCGKSSPGKLHLMKEVICIPSPGLTAKQSVELLLEIQKQVNRAMETLPPPKQETKKGHNGSKRAQPPITGKVSHLGCLTINYDAIEQPLLLLQGICSNLGLELGVNFHLAINCAGHELMDYSKGKYEVMVGTHKSAAEMVELYVDLINKYPSIIALIDPFRKEDAEQWDSLYAALASRCYLIAGAASGSVSKLLECRNISTLKSHGLIIKHTNQTTMSDLVEITHLINGKKLLAVFGSTDSESSDDSLVDLVSVKNTIFLSFKRMLLPQWLRKLLGSWTPSSPQR

Target ORF information:

RefSeq Version	XM_006527055.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus enolase 4 (Eno4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527055.3

ORF Insert Sequence:

ATGGGAGATG AAGACGGCGG CCGCAGAGGC GGGATCACTA GGGACCTGCA GAAGCTGAAG 
CAGCAGGCGA TGGCGTACTA CCAGGAGAAC GACGTCCCGC GCAAGCTGGA AGATCTGCTC 
AACTCCACCT TCTACCTCCA GCCTGCGGAT GTCTACGGGC ACCTGGCAAA CTACTTTTCT 
AAACTTGCGA AGCCTCCCTC CATATGCAAA ATAGTAGGGA AAACCATACT GGATGGCCTG 
GGACTTCCTA CTCTACAGGT GGAAATCTCC TGCACCATTC AAAATTTCCC CAAGTACATC 
TGTGCCGTGG CGATCCCTAC GCACTTTGAA GTCGTGGAGA ACGCTTTGCC AGAGGCGTTG 
GATGCAGAGG ACTCAGAAAG GGCCCAGGCC GTGAACACAG CGGTGCAGTG GATCAATCAG 
TCCATCACAG AGGAGCTGTG GGGTCTGGTC CCCTCCAACC AGGCAGAGGT GGACCACAGG 
CTCAGGACCT TCTTTGAACA TAAAGTACAA GAAGATAAAG AGAGAAAAGA ATTGGAAAAG 
AGCCAGGAGG AGCTGGTTCC CGCACCTCCA CCAGTAACGC TGCCGCCGCC GCCGCCGCCG 
CCTCCCCCAC CTCCCTCCAA AAAGAAAGGG CAGAAGGCAG GACGGAGGGA TACGCTTTTG 
GAGAAACCTG TTTCACCCCC GGAGCCCCCT GAACCAGTCC TCCATGGTAG CATGGCCATA 
GGCGCTGTGT CCCTGGCAGT GGCCAAAGCC AGCGCAACCT TGGCCAGCGA TCCTCTCTAC 
TTAACCCTGG CGTCACTGAA GCATGATCAG GAGCAGCCGT CAACATTCTC TATGCCTTTG 
CTGATGGGAT CTGTACTGAG CTGTGGGAAG TCGTCACCTG GGAAGCTACA TTTAATGAAA 
GAAGTGATTT GTATTCCCAG TCCTGGATTA ACAGCCAAAC AAAGTGTCGA GTTGCTTCTG 
GAAATTCAGA AACAAGTTAA CAGAGCGATG GAGACGCTTC CACCTCCAAA ACAAGAGACC 
AAAAAAGGGC ATAATGGGAG CAAAAGAGCT CAGCCACCGA TCACTGGCAA GGTATCTCAC 
CTTGGCTGTT TAACCATCAA CTACGACGCC ATAGAGCAGC CACTGCTGCT GCTTCAGGGG 
ATCTGCTCAA ACCTGGGGTT GGAACTGGGA GTGAACTTCC ATCTAGCCAT CAACTGTGCT 
GGGCATGAGC TGATGGACTA CAGTAAAGGG AAGTACGAAG TGATGGTGGG CACCCACAAA 
AGCGCAGCTG AGATGGTGGA GCTCTACGTG GACCTGATCA ACAAGTATCC TTCCATAATC 
GCCTTAATTG ATCCTTTCAG GAAGGAGGAC GCCGAGCAGT GGGACAGCCT CTATGCTGCT 
CTGGCTTCCC GGTGCTACCT AATTGCAGGC GCCGCTTCCG GGAGTGTCTC TAAACTCCTA 
GAGTGCAGAA ATATAAGCAC CCTGAAATCC CACGGACTGA TCATAAAGCA CACAAACCAA 
ACCACAATGT CTGACTTGGT GGAAATAACC CATCTTATCA ACGGTAAGAA GCTCCTGGCC 
GTCTTTGGAA GCACAGACTC GGAGTCCTCT GATGACAGCC TTGTCGATTT GGTTTCAGTG 
AAGAACACAA TTTTTCTTTC TTTCAAGAGG ATGCTACTGC CACAATGGCT GAGGAAACTC 
TTGGGCTCCT GGACTCCATC TTCCCCACAG AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eno4 ( XM_006527056.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527119.1 Mus musculus enolase 4 (Eno4), transcript variant X3, mRNA.

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CloneID	OMu55540
Clone ID Related Accession (Same CDS sequence)	XM_006527056.3 , XM_006527056.4
Accession Version	XM_006527056.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	enolase 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006527056.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGATG AAGACGGCGG CCGCAGAGGC GGGATCACTA GGGACCTGCA GAAGCTGAAG 
CAGCAGGCGA TGGCGTACTA CCAGGAGAAC GACGTCCCGC GCAAGCTGGA AGATCTGCTC 
AACTCCACCT TCTACCTCCA GCCTGCGGAT GTCTACGGGC ACCTGGCAAA CTACTTTTCT 
AAACTTGCGA AGCCTCCCTC CATATGCAAA ATAGTAGGGA AAACCATACT GGATGGCCTG 
GGACTTCCTA CTCTACAGGT GGAAATCTCC TGCACCATTC AAAATTTCCC CAAGTACATC 
TGTGCCGTGG CGATCCCTAC GCACTTTGAA GTCGTGGAGA ACGCTTTGCC AGAGGCGTTG 
GATGCAGAGG ACTCAGAAAG GGCCCAGGCC GTGAACACAG CGGTGCAGTG GATCAATCAG 
TCCATCACAG AGGAGCTGTG GGGTCTGGTC CCCTCCAACC AGGCAGAGGT GGACCACAGG 
CTCAGGACCT TCTTTGAACA TAAAGTACAA GAAGATAAAG AGAGAAAAGA ATTGGAAAAG 
AGCCAGGAGG AGCTGGTTCC CGCACCTCCA CCAGTAACGC TGCCGCCGCC GCCGCCGCCG 
CCTCCCCCAC CTCCCTCCAA AAAGAAAGGG CAGAAGGCAG GACGGAGGGA TACGCTTTTG 
GAGAAACCTG TTTCACCCCC GGAGCCCCCT GAACCAGTCC TCCATGGTAG CATGGCCATA 
GGCGCTGTGT CCCTGGCAGT GGCCAAAGCC AGCGCAACCT TGGCCAGCGA TCCTCTCTAC 
TTAACCCTGG CGTCACTGAA GCATGATCAG GAGCAGCCGT CAACATTCTC TATGCCTTTG 
CTGATGGGAT CTGTACTGAG CTGTGGGAAG TCGTCACCTG GGAAGCTACA TTTAATGAAA 
GAAGTGATTT GTATTCCCAG TCCTGGATTA ACAGCCAAAC AAAGTGTCGA GTTGCTTCTG 
GAAATTCAGA AACAAGTTAA CAGAGCGATG GAGACGCTTC CACCTCCAAA ACAAGAGACC 
AAAAAAGGGC ATAATGGGAG CAAAAGAGCT CAGCCACCGA TCACTGGCAA GGTATCTCAC 
CTTGGCTGTT TAACCATCAA CTACGACGCC ATAGAGCAGC CACTGCTGCT GCTTCAGGGG 
ATCTGCTCAA ACCTGGGGTT GGAACTGGGA GTGAACTTCC ATCTAGCCAT CAACTGTGCT 
GGGCATGAGC TGATGGACTA CAGTAAAGGG AAGTACGAAG TGATGGTGGG CACCCACAAA 
AGCGCAGCTG AGATGGTGGA GCTCTACGTG GACCTGATCA ACAAGTATCC TTCCATAATC 
GCCTTAATTG ATCCTTTCAG GAAGGAGGAC GCCGAGCAGT GGGACAGCCT CTATGCTGCT 
CTGGCTTCCC GGTGCTACCT AATTGCAGGC GCCGCTTCCG GGAGTGTCTC TAAACTCCTA 
GAGTGCAGAA ATATAAGCAC CCTGAAATCC CACGGACTGA TCATAAAGCA CACAAACCAA 
ACCACAATGT CTGACTTGGT GGAAATAACC CATCTTATCA ACGGTAAGAA GCTCCTGGCC 
GTCTTTGGAA GCACAGACTC GGAGTCCTCT GATGACAGCC TTGTCGATTT GAGGATGCTA 
CTGCCACAAT GGCTGAGGAA ACTCTTGGGC TCCTGGACTC CATCTTCCCC ACAGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006527119.1
CDS	8274..9953
Translation	MGDEDGGRRGGITRDLQKLKQQAMAYYQENDVPRKLEDLLNSTFYLQPADVYGHLANYFSKLAKPPSICKIVGKTILDGLGLPTLQVEISCTIQNFPKYICAVAIPTHFEVVENALPEALDAEDSERAQAVNTAVQWINQSITEELWGLVPSNQAEVDHRLRTFFEHKVQEDKERKELEKSQEELVPAPPPVTLPPPPPPPPPPPSKKKGQKAGRRDTLLEKPVSPPEPPEPVLHGSMAIGAVSLAVAKASATLASDPLYLTLASLKHDQEQPSTFSMPLLMGSVLSCGKSSPGKLHLMKEVICIPSPGLTAKQSVELLLEIQKQVNRAMETLPPPKQETKKGHNGSKRAQPPITGKVSHLGCLTINYDAIEQPLLLLQGICSNLGLELGVNFHLAINCAGHELMDYSKGKYEVMVGTHKSAAEMVELYVDLINKYPSIIALIDPFRKEDAEQWDSLYAALASRCYLIAGAASGSVSKLLECRNISTLKSHGLIIKHTNQTTMSDLVEITHLINGKKLLAVFGSTDSESSDDSLVDLRMLLPQWLRKLLGSWTPSSPQR

Target ORF information:

RefSeq Version	XM_006527056.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus enolase 4 (Eno4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527056.3

ORF Insert Sequence:

ATGGGAGATG AAGACGGCGG CCGCAGAGGC GGGATCACTA GGGACCTGCA GAAGCTGAAG 
CAGCAGGCGA TGGCGTACTA CCAGGAGAAC GACGTCCCGC GCAAGCTGGA AGATCTGCTC 
AACTCCACCT TCTACCTCCA GCCTGCGGAT GTCTACGGGC ACCTGGCAAA CTACTTTTCT 
AAACTTGCGA AGCCTCCCTC CATATGCAAA ATAGTAGGGA AAACCATACT GGATGGCCTG 
GGACTTCCTA CTCTACAGGT GGAAATCTCC TGCACCATTC AAAATTTCCC CAAGTACATC 
TGTGCCGTGG CGATCCCTAC GCACTTTGAA GTCGTGGAGA ACGCTTTGCC AGAGGCGTTG 
GATGCAGAGG ACTCAGAAAG GGCCCAGGCC GTGAACACAG CGGTGCAGTG GATCAATCAG 
TCCATCACAG AGGAGCTGTG GGGTCTGGTC CCCTCCAACC AGGCAGAGGT GGACCACAGG 
CTCAGGACCT TCTTTGAACA TAAAGTACAA GAAGATAAAG AGAGAAAAGA ATTGGAAAAG 
AGCCAGGAGG AGCTGGTTCC CGCACCTCCA CCAGTAACGC TGCCGCCGCC GCCGCCGCCG 
CCTCCCCCAC CTCCCTCCAA AAAGAAAGGG CAGAAGGCAG GACGGAGGGA TACGCTTTTG 
GAGAAACCTG TTTCACCCCC GGAGCCCCCT GAACCAGTCC TCCATGGTAG CATGGCCATA 
GGCGCTGTGT CCCTGGCAGT GGCCAAAGCC AGCGCAACCT TGGCCAGCGA TCCTCTCTAC 
TTAACCCTGG CGTCACTGAA GCATGATCAG GAGCAGCCGT CAACATTCTC TATGCCTTTG 
CTGATGGGAT CTGTACTGAG CTGTGGGAAG TCGTCACCTG GGAAGCTACA TTTAATGAAA 
GAAGTGATTT GTATTCCCAG TCCTGGATTA ACAGCCAAAC AAAGTGTCGA GTTGCTTCTG 
GAAATTCAGA AACAAGTTAA CAGAGCGATG GAGACGCTTC CACCTCCAAA ACAAGAGACC 
AAAAAAGGGC ATAATGGGAG CAAAAGAGCT CAGCCACCGA TCACTGGCAA GGTATCTCAC 
CTTGGCTGTT TAACCATCAA CTACGACGCC ATAGAGCAGC CACTGCTGCT GCTTCAGGGG 
ATCTGCTCAA ACCTGGGGTT GGAACTGGGA GTGAACTTCC ATCTAGCCAT CAACTGTGCT 
GGGCATGAGC TGATGGACTA CAGTAAAGGG AAGTACGAAG TGATGGTGGG CACCCACAAA 
AGCGCAGCTG AGATGGTGGA GCTCTACGTG GACCTGATCA ACAAGTATCC TTCCATAATC 
GCCTTAATTG ATCCTTTCAG GAAGGAGGAC GCCGAGCAGT GGGACAGCCT CTATGCTGCT 
CTGGCTTCCC GGTGCTACCT AATTGCAGGC GCCGCTTCCG GGAGTGTCTC TAAACTCCTA 
GAGTGCAGAA ATATAAGCAC CCTGAAATCC CACGGACTGA TCATAAAGCA CACAAACCAA 
ACCACAATGT CTGACTTGGT GGAAATAACC CATCTTATCA ACGGTAAGAA GCTCCTGGCC 
GTCTTTGGAA GCACAGACTC GGAGTCCTCT GATGACAGCC TTGTCGATTT GAGGATGCTA 
CTGCCACAAT GGCTGAGGAA ACTCTTGGGC TCCTGGACTC CATCTTCCCC ACAGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eno4 ( XM_030250922.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030106782.1 Mus musculus enolase 4 (Eno4), transcript variant X4, mRNA.

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CloneID	OMu87571
Clone ID Related Accession (Same CDS sequence)	XM_030250922.1
Accession Version	XM_030250922.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	enolase 4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCATAG GCGCTGTGTC CCTGGCAGTG GCCAAAGCCA GCGCAACCTT GGCCAGCGAT 
CCTCTCTACT TAACCCTGGC GTCACTGAAG CATGATCAGG AGCAGCCGTC AACATTCTCT 
ATGCCTTTGC TGATGGGATC TGTACTGAGC TGTGGGAAGT CGTCACCTGG GAAGCTACAT 
TTAATGAAAG AAGTGATTTG TATTCCCAGT CCTGGATTAA CAGCCAAACA AAGTGTCGAG 
TTGCTTCTGG AAATTCAGAA ACAAGTTAAC AGAGCGATGG AGACGCTTCC ACCTCCAAAA 
CAAGAGACCA AAAAAGGGCA TAATGGGAGC AAAAGAGCTC AGCCACCGAT CACTGGCAAG 
GTATCTCACC TTGGCTGTTT AACCATCAAC TACGACGCCA TAGAGCAGCC ACTGCTGCTG 
CTTCAGGGGA TCTGCTCAAA CCTGGGGTTG GAACTGGGAG TGAACTTCCA TCTAGCCATC 
AACTGTGCTG GGCATGAGCT GATGGACTAC AGTAAAGGGA AGTACGAAGT GATGGTGGGC 
ACCCACAAAA GCGCAGCTGA GATGGTGGAG CTCTACGTGG ACCTGATCAA CAAGTATCCT 
TCCATAATCG CCTTAATTGA TCCTTTCAGG AAGGAGGACG CCGAGCAGTG GGACAGCCTC 
TATGCTGCTC TGGCTTCCCG GTGCTACCTA ATTGCAGGCG CCGCTTCCGG GAGTGTCTCT 
AAACTCCTAG AGTGCAGAAA TATAAGCACC CTGAAATCCC ACGGACTGAT CATAAAGCAC 
ACAAACCAAA CCACAATGTC TGACTTGGTG GAAATAACCC ATCTTATCAA CGGTAAGAAG 
CTCCTGGCCG TCTTTGGAAG CACAGACTCG GAGTCCTCTG ATGACAGCCT TGTCGATTTG 
GCTGTTGGAT TCGGTGCCCG GTTTATCAAG TTGGGGGGTC TTTCTCGGGG TGAACGGATG 
ACCAAATACA ACCGCCTTCT TGCTATAGAG GAAGAACTCA TCCAGAGGGG AGTATGGGGT 
TTCAGTGAAG AACACAATTT TTCTTTCTTT CAAGAGGATG CTACTGCCAC AATGGCTGAG 
GAAACTCTTG GGCTCCTGGA CTCCATCTTC CCCACAGAGG TGATAGAGGA ATCGGCTAAA 
ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030106782.1
CDS	91..1236
Translation	MAIGAVSLAVAKASATLASDPLYLTLASLKHDQEQPSTFSMPLLMGSVLSCGKSSPGKLHLMKEVICIPSPGLTAKQSVELLLEIQKQVNRAMETLPPPKQETKKGHNGSKRAQPPITGKVSHLGCLTINYDAIEQPLLLLQGICSNLGLELGVNFHLAINCAGHELMDYSKGKYEVMVGTHKSAAEMVELYVDLINKYPSIIALIDPFRKEDAEQWDSLYAALASRCYLIAGAASGSVSKLLECRNISTLKSHGLIIKHTNQTTMSDLVEITHLINGKKLLAVFGSTDSESSDDSLVDLAVGFGARFIKLGGLSRGERMTKYNRLLAIEEELIQRGVWGFSEEHNFSFFQEDATATMAEETLGLLDSIFPTEVIEESAKT

Target ORF information:

RefSeq Version	XM_030250922.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus enolase 4 (Eno4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030250922.1

ORF Insert Sequence:

ATGGCCATAG GCGCTGTGTC CCTGGCAGTG GCCAAAGCCA GCGCAACCTT GGCCAGCGAT 
CCTCTCTACT TAACCCTGGC GTCACTGAAG CATGATCAGG AGCAGCCGTC AACATTCTCT 
ATGCCTTTGC TGATGGGATC TGTACTGAGC TGTGGGAAGT CGTCACCTGG GAAGCTACAT 
TTAATGAAAG AAGTGATTTG TATTCCCAGT CCTGGATTAA CAGCCAAACA AAGTGTCGAG 
TTGCTTCTGG AAATTCAGAA ACAAGTTAAC AGAGCGATGG AGACGCTTCC ACCTCCAAAA 
CAAGAGACCA AAAAAGGGCA TAATGGGAGC AAAAGAGCTC AGCCACCGAT CACTGGCAAG 
GTATCTCACC TTGGCTGTTT AACCATCAAC TACGACGCCA TAGAGCAGCC ACTGCTGCTG 
CTTCAGGGGA TCTGCTCAAA CCTGGGGTTG GAACTGGGAG TGAACTTCCA TCTAGCCATC 
AACTGTGCTG GGCATGAGCT GATGGACTAC AGTAAAGGGA AGTACGAAGT GATGGTGGGC 
ACCCACAAAA GCGCAGCTGA GATGGTGGAG CTCTACGTGG ACCTGATCAA CAAGTATCCT 
TCCATAATCG CCTTAATTGA TCCTTTCAGG AAGGAGGACG CCGAGCAGTG GGACAGCCTC 
TATGCTGCTC TGGCTTCCCG GTGCTACCTA ATTGCAGGCG CCGCTTCCGG GAGTGTCTCT 
AAACTCCTAG AGTGCAGAAA TATAAGCACC CTGAAATCCC ACGGACTGAT CATAAAGCAC 
ACAAACCAAA CCACAATGTC TGACTTGGTG GAAATAACCC ATCTTATCAA CGGTAAGAAG 
CTCCTGGCCG TCTTTGGAAG CACAGACTCG GAGTCCTCTG ATGACAGCCT TGTCGATTTG 
GCTGTTGGAT TCGGTGCCCG GTTTATCAAG TTGGGGGGTC TTTCTCGGGG TGAACGGATG 
ACCAAATACA ACCGCCTTCT TGCTATAGAG GAAGAACTCA TCCAGAGGGG AGTATGGGGT 
TTCAGTGAAG AACACAATTT TTCTTTCTTT CAAGAGGATG CTACTGCCAC AATGGCTGAG 
GAAACTCTTG GGCTCCTGGA CTCCATCTTC CCCACAGAGG TGATAGAGGA ATCGGCTAAA 
ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eno4 ( XM_006527054.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527117.1 Mus musculus enolase 4 (Eno4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu55538
Clone ID Related Accession (Same CDS sequence)	XM_006527054.3 , XM_006527054.4
Accession Version	XM_006527054.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	enolase 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006527054.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGATG AAGACGGCGG CCGCAGAGGC GGGATCACTA GGGACCTGCA GAAGCTGAAG 
CAGCAGGCGA TGGCGTACTA CCAGGAGAAC GACGTCCCGC GCAAGCTGGA AGATCTGCTC 
AACTCCACCT TCTACCTCCA GCCTGCGGAT GTCTACGGGC ACCTGGCAAA CTACTTTTCT 
AAACTTGCGA AGCCTCCCTC CATATGCAAA ATAGTAGGGA AAACCATACT GGATGGCCTG 
GGACTTCCTA CTCTACAGGT GGAAATCTCC TGCACCATTC AAAATTTCCC CAAGTACATC 
TGTGCCGTGG CGATCCCTAC GCACTTTGAA GTCGTGGAGA ACGCTTTGCC AGAGGCGTTG 
GATGCAGAGG ACTCAGAAAG GGCCCAGGCC GTGAACACAG CGGTGCAGTG GATCAATCAG 
TCCATCACAG AGGAGCTGTG GGGTCTGGTC CCCTCCAACC AGGCAGAGGT GGACCACAGG 
CTCAGGACCT TCTTTGAACA TAAAGTACAA GAAGATAAAG AGAGAAAAGA ATTGGAAAAG 
AGCCAGGAGG AGCTGGTTCC CGCACCTCCA CCAGTAACGC TGCCGCCGCC GCCGCCGCCG 
CCTCCCCCAC CTCCCTCCAA AAAGAAAGGG CAGAAGGCAG GACGGAGGGA TACGCTTTTG 
GAGAAACCTG TTTCACCCCC GGAGCCCCCT GAACCAGTCC TCCATGGTAG CATGGCCATA 
GGCGCTGTGT CCCTGGCAGT GGCCAAAGCC AGCGCAACCT TGGCCAGCGA TCCTCTCTAC 
TTAACCCTGG CGTCACTGAA GCATGATCAG GAGCAGCCGT CAACATTCTC TATGCCTTTG 
CTGATGGGAT CTGTACTGAG CTGTGGGAAG TCGTCACCTG GGAAGCTACA TTTAATGAAA 
GAAGTGATTT GTATTCCCAG TCCTGGATTA ACAGCCAAAC AAAGTGTCGA GTTGCTTCTG 
GAAATTCAGA AACAAGTTAA CAGAGCGATG GAGACGCTTC CACCTCCAAA ACAAGAGACC 
AAAAAAGGGC ATAATGGGAG CAAAAGAGCT CAGCCACCGA TCACTGGCAA GGTATCTCAC 
CTTGGCTGTT TAACCATCAA CTACGACGCC ATAGAGCAGC CACTGCTGCT GCTTCAGGGG 
ATCTGCTCAA ACCTGGGGTT GGAACTGGGA GTGAACTTCC ATCTAGCCAT CAACTGTGCT 
GGGCATGAGC TGATGGACTA CAGTAAAGGG AAGTACGAAG TGATGGTGGG CACCCACAAA 
AGCGCAGCTG AGATGGTGGA GCTCTACGTG GACCTGATCA ACAAGTATCC TTCCATAATC 
GCCTTAATTG ATCCTTTCAG GAAGGAGGAC GCCGAGCAGT GGGACAGCCT CTATGCTGCT 
CTGGCTTCCC GGTGCTACCT AATTGCAGGC GCCGCTTCCG GGAGTGTCTC TAAACTCCTA 
GAGTGCAGAA ATATAAGCAC CCTGAAATCC CACGGACTGA TCATAAAGCA CACAAACCAA 
ACCACAATGT CTGACTTGGT GGAAATAACC CATCTTATCA ACGGTAAGAA GCTCCTGGCC 
GTCTTTGGAA GCACAGACTC GGAGTCCTCT GATGACAGCC TTGTCGATTT GGCTGTTGGA 
TTCGGTGCCC GGTTTATCAA GTTGGGGGGT CTTTCTCGGG GTGAACGGAT GACCAAATAC 
AACCGCCTTC TTGCTATAGA GGAAGAACTC ATCCAGAGGG GAGTATGGGA GGATGCTACT 
GCCACAATGG CTGAGGAAAC TCTTGGGCTC CTGGACTCCA TCTTCCCCAC AGAGGTGATA 
GAGGAATCGG CTAAAACATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006527117.1
CDS	9400..11220
Translation	MGDEDGGRRGGITRDLQKLKQQAMAYYQENDVPRKLEDLLNSTFYLQPADVYGHLANYFSKLAKPPSICKIVGKTILDGLGLPTLQVEISCTIQNFPKYICAVAIPTHFEVVENALPEALDAEDSERAQAVNTAVQWINQSITEELWGLVPSNQAEVDHRLRTFFEHKVQEDKERKELEKSQEELVPAPPPVTLPPPPPPPPPPPSKKKGQKAGRRDTLLEKPVSPPEPPEPVLHGSMAIGAVSLAVAKASATLASDPLYLTLASLKHDQEQPSTFSMPLLMGSVLSCGKSSPGKLHLMKEVICIPSPGLTAKQSVELLLEIQKQVNRAMETLPPPKQETKKGHNGSKRAQPPITGKVSHLGCLTINYDAIEQPLLLLQGICSNLGLELGVNFHLAINCAGHELMDYSKGKYEVMVGTHKSAAEMVELYVDLINKYPSIIALIDPFRKEDAEQWDSLYAALASRCYLIAGAASGSVSKLLECRNISTLKSHGLIIKHTNQTTMSDLVEITHLINGKKLLAVFGSTDSESSDDSLVDLAVGFGARFIKLGGLSRGERMTKYNRLLAIEEELIQRGVWEDATATMAEETLGLLDSIFPTEVIEESAKT

Target ORF information:

RefSeq Version	XM_006527054.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus enolase 4 (Eno4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527054.4

ORF Insert Sequence:

ATGGGAGATG AAGACGGCGG CCGCAGAGGC GGGATCACTA GGGACCTGCA GAAGCTGAAG 
CAGCAGGCGA TGGCGTACTA CCAGGAGAAC GACGTCCCGC GCAAGCTGGA AGATCTGCTC 
AACTCCACCT TCTACCTCCA GCCTGCGGAT GTCTACGGGC ACCTGGCAAA CTACTTTTCT 
AAACTTGCGA AGCCTCCCTC CATATGCAAA ATAGTAGGGA AAACCATACT GGATGGCCTG 
GGACTTCCTA CTCTACAGGT GGAAATCTCC TGCACCATTC AAAATTTCCC CAAGTACATC 
TGTGCCGTGG CGATCCCTAC GCACTTTGAA GTCGTGGAGA ACGCTTTGCC AGAGGCGTTG 
GATGCAGAGG ACTCAGAAAG GGCCCAGGCC GTGAACACAG CGGTGCAGTG GATCAATCAG 
TCCATCACAG AGGAGCTGTG GGGTCTGGTC CCCTCCAACC AGGCAGAGGT GGACCACAGG 
CTCAGGACCT TCTTTGAACA TAAAGTACAA GAAGATAAAG AGAGAAAAGA ATTGGAAAAG 
AGCCAGGAGG AGCTGGTTCC CGCACCTCCA CCAGTAACGC TGCCGCCGCC GCCGCCGCCG 
CCTCCCCCAC CTCCCTCCAA AAAGAAAGGG CAGAAGGCAG GACGGAGGGA TACGCTTTTG 
GAGAAACCTG TTTCACCCCC GGAGCCCCCT GAACCAGTCC TCCATGGTAG CATGGCCATA 
GGCGCTGTGT CCCTGGCAGT GGCCAAAGCC AGCGCAACCT TGGCCAGCGA TCCTCTCTAC 
TTAACCCTGG CGTCACTGAA GCATGATCAG GAGCAGCCGT CAACATTCTC TATGCCTTTG 
CTGATGGGAT CTGTACTGAG CTGTGGGAAG TCGTCACCTG GGAAGCTACA TTTAATGAAA 
GAAGTGATTT GTATTCCCAG TCCTGGATTA ACAGCCAAAC AAAGTGTCGA GTTGCTTCTG 
GAAATTCAGA AACAAGTTAA CAGAGCGATG GAGACGCTTC CACCTCCAAA ACAAGAGACC 
AAAAAAGGGC ATAATGGGAG CAAAAGAGCT CAGCCACCGA TCACTGGCAA GGTATCTCAC 
CTTGGCTGTT TAACCATCAA CTACGACGCC ATAGAGCAGC CACTGCTGCT GCTTCAGGGG 
ATCTGCTCAA ACCTGGGGTT GGAACTGGGA GTGAACTTCC ATCTAGCCAT CAACTGTGCT 
GGGCATGAGC TGATGGACTA CAGTAAAGGG AAGTACGAAG TGATGGTGGG CACCCACAAA 
AGCGCAGCTG AGATGGTGGA GCTCTACGTG GACCTGATCA ACAAGTATCC TTCCATAATC 
GCCTTAATTG ATCCTTTCAG GAAGGAGGAC GCCGAGCAGT GGGACAGCCT CTATGCTGCT 
CTGGCTTCCC GGTGCTACCT AATTGCAGGC GCCGCTTCCG GGAGTGTCTC TAAACTCCTA 
GAGTGCAGAA ATATAAGCAC CCTGAAATCC CACGGACTGA TCATAAAGCA CACAAACCAA 
ACCACAATGT CTGACTTGGT GGAAATAACC CATCTTATCA ACGGTAAGAA GCTCCTGGCC 
GTCTTTGGAA GCACAGACTC GGAGTCCTCT GATGACAGCC TTGTCGATTT GGCTGTTGGA 
TTCGGTGCCC GGTTTATCAA GTTGGGGGGT CTTTCTCGGG GTGAACGGAT GACCAAATAC 
AACCGCCTTC TTGCTATAGA GGAAGAACTC ATCCAGAGGG GAGTATGGGA GGATGCTACT 
GCCACAATGG CTGAGGAAAC TCTTGGGCTC CTGGACTCCA TCTTCCCCAC AGAGGTGATA 
GAGGAATCGG CTAAAACATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eno4 ( XM_006527055.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527118.1 Mus musculus enolase 4 (Eno4), transcript variant X2, mRNA.

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CloneID	OMu55539
Clone ID Related Accession (Same CDS sequence)	XM_006527055.4 , XM_006527055.3
Accession Version	XM_006527055.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1716bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	enolase 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006527055.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGATG AAGACGGCGG CCGCAGAGGC GGGATCACTA GGGACCTGCA GAAGCTGAAG 
CAGCAGGCGA TGGCGTACTA CCAGGAGAAC GACGTCCCGC GCAAGCTGGA AGATCTGCTC 
AACTCCACCT TCTACCTCCA GCCTGCGGAT GTCTACGGGC ACCTGGCAAA CTACTTTTCT 
AAACTTGCGA AGCCTCCCTC CATATGCAAA ATAGTAGGGA AAACCATACT GGATGGCCTG 
GGACTTCCTA CTCTACAGGT GGAAATCTCC TGCACCATTC AAAATTTCCC CAAGTACATC 
TGTGCCGTGG CGATCCCTAC GCACTTTGAA GTCGTGGAGA ACGCTTTGCC AGAGGCGTTG 
GATGCAGAGG ACTCAGAAAG GGCCCAGGCC GTGAACACAG CGGTGCAGTG GATCAATCAG 
TCCATCACAG AGGAGCTGTG GGGTCTGGTC CCCTCCAACC AGGCAGAGGT GGACCACAGG 
CTCAGGACCT TCTTTGAACA TAAAGTACAA GAAGATAAAG AGAGAAAAGA ATTGGAAAAG 
AGCCAGGAGG AGCTGGTTCC CGCACCTCCA CCAGTAACGC TGCCGCCGCC GCCGCCGCCG 
CCTCCCCCAC CTCCCTCCAA AAAGAAAGGG CAGAAGGCAG GACGGAGGGA TACGCTTTTG 
GAGAAACCTG TTTCACCCCC GGAGCCCCCT GAACCAGTCC TCCATGGTAG CATGGCCATA 
GGCGCTGTGT CCCTGGCAGT GGCCAAAGCC AGCGCAACCT TGGCCAGCGA TCCTCTCTAC 
TTAACCCTGG CGTCACTGAA GCATGATCAG GAGCAGCCGT CAACATTCTC TATGCCTTTG 
CTGATGGGAT CTGTACTGAG CTGTGGGAAG TCGTCACCTG GGAAGCTACA TTTAATGAAA 
GAAGTGATTT GTATTCCCAG TCCTGGATTA ACAGCCAAAC AAAGTGTCGA GTTGCTTCTG 
GAAATTCAGA AACAAGTTAA CAGAGCGATG GAGACGCTTC CACCTCCAAA ACAAGAGACC 
AAAAAAGGGC ATAATGGGAG CAAAAGAGCT CAGCCACCGA TCACTGGCAA GGTATCTCAC 
CTTGGCTGTT TAACCATCAA CTACGACGCC ATAGAGCAGC CACTGCTGCT GCTTCAGGGG 
ATCTGCTCAA ACCTGGGGTT GGAACTGGGA GTGAACTTCC ATCTAGCCAT CAACTGTGCT 
GGGCATGAGC TGATGGACTA CAGTAAAGGG AAGTACGAAG TGATGGTGGG CACCCACAAA 
AGCGCAGCTG AGATGGTGGA GCTCTACGTG GACCTGATCA ACAAGTATCC TTCCATAATC 
GCCTTAATTG ATCCTTTCAG GAAGGAGGAC GCCGAGCAGT GGGACAGCCT CTATGCTGCT 
CTGGCTTCCC GGTGCTACCT AATTGCAGGC GCCGCTTCCG GGAGTGTCTC TAAACTCCTA 
GAGTGCAGAA ATATAAGCAC CCTGAAATCC CACGGACTGA TCATAAAGCA CACAAACCAA 
ACCACAATGT CTGACTTGGT GGAAATAACC CATCTTATCA ACGGTAAGAA GCTCCTGGCC 
GTCTTTGGAA GCACAGACTC GGAGTCCTCT GATGACAGCC TTGTCGATTT GGTTTCAGTG 
AAGAACACAA TTTTTCTTTC TTTCAAGAGG ATGCTACTGC CACAATGGCT GAGGAAACTC 
TTGGGCTCCT GGACTCCATC TTCCCCACAG AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006527118.1
CDS	9400..11115
Translation	MGDEDGGRRGGITRDLQKLKQQAMAYYQENDVPRKLEDLLNSTFYLQPADVYGHLANYFSKLAKPPSICKIVGKTILDGLGLPTLQVEISCTIQNFPKYICAVAIPTHFEVVENALPEALDAEDSERAQAVNTAVQWINQSITEELWGLVPSNQAEVDHRLRTFFEHKVQEDKERKELEKSQEELVPAPPPVTLPPPPPPPPPPPSKKKGQKAGRRDTLLEKPVSPPEPPEPVLHGSMAIGAVSLAVAKASATLASDPLYLTLASLKHDQEQPSTFSMPLLMGSVLSCGKSSPGKLHLMKEVICIPSPGLTAKQSVELLLEIQKQVNRAMETLPPPKQETKKGHNGSKRAQPPITGKVSHLGCLTINYDAIEQPLLLLQGICSNLGLELGVNFHLAINCAGHELMDYSKGKYEVMVGTHKSAAEMVELYVDLINKYPSIIALIDPFRKEDAEQWDSLYAALASRCYLIAGAASGSVSKLLECRNISTLKSHGLIIKHTNQTTMSDLVEITHLINGKKLLAVFGSTDSESSDDSLVDLVSVKNTIFLSFKRMLLPQWLRKLLGSWTPSSPQR

Target ORF information:

RefSeq Version	XM_006527055.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus enolase 4 (Eno4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527055.4

ORF Insert Sequence:

ATGGGAGATG AAGACGGCGG CCGCAGAGGC GGGATCACTA GGGACCTGCA GAAGCTGAAG 
CAGCAGGCGA TGGCGTACTA CCAGGAGAAC GACGTCCCGC GCAAGCTGGA AGATCTGCTC 
AACTCCACCT TCTACCTCCA GCCTGCGGAT GTCTACGGGC ACCTGGCAAA CTACTTTTCT 
AAACTTGCGA AGCCTCCCTC CATATGCAAA ATAGTAGGGA AAACCATACT GGATGGCCTG 
GGACTTCCTA CTCTACAGGT GGAAATCTCC TGCACCATTC AAAATTTCCC CAAGTACATC 
TGTGCCGTGG CGATCCCTAC GCACTTTGAA GTCGTGGAGA ACGCTTTGCC AGAGGCGTTG 
GATGCAGAGG ACTCAGAAAG GGCCCAGGCC GTGAACACAG CGGTGCAGTG GATCAATCAG 
TCCATCACAG AGGAGCTGTG GGGTCTGGTC CCCTCCAACC AGGCAGAGGT GGACCACAGG 
CTCAGGACCT TCTTTGAACA TAAAGTACAA GAAGATAAAG AGAGAAAAGA ATTGGAAAAG 
AGCCAGGAGG AGCTGGTTCC CGCACCTCCA CCAGTAACGC TGCCGCCGCC GCCGCCGCCG 
CCTCCCCCAC CTCCCTCCAA AAAGAAAGGG CAGAAGGCAG GACGGAGGGA TACGCTTTTG 
GAGAAACCTG TTTCACCCCC GGAGCCCCCT GAACCAGTCC TCCATGGTAG CATGGCCATA 
GGCGCTGTGT CCCTGGCAGT GGCCAAAGCC AGCGCAACCT TGGCCAGCGA TCCTCTCTAC 
TTAACCCTGG CGTCACTGAA GCATGATCAG GAGCAGCCGT CAACATTCTC TATGCCTTTG 
CTGATGGGAT CTGTACTGAG CTGTGGGAAG TCGTCACCTG GGAAGCTACA TTTAATGAAA 
GAAGTGATTT GTATTCCCAG TCCTGGATTA ACAGCCAAAC AAAGTGTCGA GTTGCTTCTG 
GAAATTCAGA AACAAGTTAA CAGAGCGATG GAGACGCTTC CACCTCCAAA ACAAGAGACC 
AAAAAAGGGC ATAATGGGAG CAAAAGAGCT CAGCCACCGA TCACTGGCAA GGTATCTCAC 
CTTGGCTGTT TAACCATCAA CTACGACGCC ATAGAGCAGC CACTGCTGCT GCTTCAGGGG 
ATCTGCTCAA ACCTGGGGTT GGAACTGGGA GTGAACTTCC ATCTAGCCAT CAACTGTGCT 
GGGCATGAGC TGATGGACTA CAGTAAAGGG AAGTACGAAG TGATGGTGGG CACCCACAAA 
AGCGCAGCTG AGATGGTGGA GCTCTACGTG GACCTGATCA ACAAGTATCC TTCCATAATC 
GCCTTAATTG ATCCTTTCAG GAAGGAGGAC GCCGAGCAGT GGGACAGCCT CTATGCTGCT 
CTGGCTTCCC GGTGCTACCT AATTGCAGGC GCCGCTTCCG GGAGTGTCTC TAAACTCCTA 
GAGTGCAGAA ATATAAGCAC CCTGAAATCC CACGGACTGA TCATAAAGCA CACAAACCAA 
ACCACAATGT CTGACTTGGT GGAAATAACC CATCTTATCA ACGGTAAGAA GCTCCTGGCC 
GTCTTTGGAA GCACAGACTC GGAGTCCTCT GATGACAGCC TTGTCGATTT GGTTTCAGTG 
AAGAACACAA TTTTTCTTTC TTTCAAGAGG ATGCTACTGC CACAATGGCT GAGGAAACTC 
TTGGGCTCCT GGACTCCATC TTCCCCACAG AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Eno4 ( XM_006527056.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527119.1 Mus musculus enolase 4 (Eno4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu55540
Clone ID Related Accession (Same CDS sequence)	XM_006527056.3 , XM_006527056.4
Accession Version	XM_006527056.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	enolase 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006527056.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGATG AAGACGGCGG CCGCAGAGGC GGGATCACTA GGGACCTGCA GAAGCTGAAG 
CAGCAGGCGA TGGCGTACTA CCAGGAGAAC GACGTCCCGC GCAAGCTGGA AGATCTGCTC 
AACTCCACCT TCTACCTCCA GCCTGCGGAT GTCTACGGGC ACCTGGCAAA CTACTTTTCT 
AAACTTGCGA AGCCTCCCTC CATATGCAAA ATAGTAGGGA AAACCATACT GGATGGCCTG 
GGACTTCCTA CTCTACAGGT GGAAATCTCC TGCACCATTC AAAATTTCCC CAAGTACATC 
TGTGCCGTGG CGATCCCTAC GCACTTTGAA GTCGTGGAGA ACGCTTTGCC AGAGGCGTTG 
GATGCAGAGG ACTCAGAAAG GGCCCAGGCC GTGAACACAG CGGTGCAGTG GATCAATCAG 
TCCATCACAG AGGAGCTGTG GGGTCTGGTC CCCTCCAACC AGGCAGAGGT GGACCACAGG 
CTCAGGACCT TCTTTGAACA TAAAGTACAA GAAGATAAAG AGAGAAAAGA ATTGGAAAAG 
AGCCAGGAGG AGCTGGTTCC CGCACCTCCA CCAGTAACGC TGCCGCCGCC GCCGCCGCCG 
CCTCCCCCAC CTCCCTCCAA AAAGAAAGGG CAGAAGGCAG GACGGAGGGA TACGCTTTTG 
GAGAAACCTG TTTCACCCCC GGAGCCCCCT GAACCAGTCC TCCATGGTAG CATGGCCATA 
GGCGCTGTGT CCCTGGCAGT GGCCAAAGCC AGCGCAACCT TGGCCAGCGA TCCTCTCTAC 
TTAACCCTGG CGTCACTGAA GCATGATCAG GAGCAGCCGT CAACATTCTC TATGCCTTTG 
CTGATGGGAT CTGTACTGAG CTGTGGGAAG TCGTCACCTG GGAAGCTACA TTTAATGAAA 
GAAGTGATTT GTATTCCCAG TCCTGGATTA ACAGCCAAAC AAAGTGTCGA GTTGCTTCTG 
GAAATTCAGA AACAAGTTAA CAGAGCGATG GAGACGCTTC CACCTCCAAA ACAAGAGACC 
AAAAAAGGGC ATAATGGGAG CAAAAGAGCT CAGCCACCGA TCACTGGCAA GGTATCTCAC 
CTTGGCTGTT TAACCATCAA CTACGACGCC ATAGAGCAGC CACTGCTGCT GCTTCAGGGG 
ATCTGCTCAA ACCTGGGGTT GGAACTGGGA GTGAACTTCC ATCTAGCCAT CAACTGTGCT 
GGGCATGAGC TGATGGACTA CAGTAAAGGG AAGTACGAAG TGATGGTGGG CACCCACAAA 
AGCGCAGCTG AGATGGTGGA GCTCTACGTG GACCTGATCA ACAAGTATCC TTCCATAATC 
GCCTTAATTG ATCCTTTCAG GAAGGAGGAC GCCGAGCAGT GGGACAGCCT CTATGCTGCT 
CTGGCTTCCC GGTGCTACCT AATTGCAGGC GCCGCTTCCG GGAGTGTCTC TAAACTCCTA 
GAGTGCAGAA ATATAAGCAC CCTGAAATCC CACGGACTGA TCATAAAGCA CACAAACCAA 
ACCACAATGT CTGACTTGGT GGAAATAACC CATCTTATCA ACGGTAAGAA GCTCCTGGCC 
GTCTTTGGAA GCACAGACTC GGAGTCCTCT GATGACAGCC TTGTCGATTT GAGGATGCTA 
CTGCCACAAT GGCTGAGGAA ACTCTTGGGC TCCTGGACTC CATCTTCCCC ACAGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006527119.1
CDS	9400..11079
Translation	MGDEDGGRRGGITRDLQKLKQQAMAYYQENDVPRKLEDLLNSTFYLQPADVYGHLANYFSKLAKPPSICKIVGKTILDGLGLPTLQVEISCTIQNFPKYICAVAIPTHFEVVENALPEALDAEDSERAQAVNTAVQWINQSITEELWGLVPSNQAEVDHRLRTFFEHKVQEDKERKELEKSQEELVPAPPPVTLPPPPPPPPPPPSKKKGQKAGRRDTLLEKPVSPPEPPEPVLHGSMAIGAVSLAVAKASATLASDPLYLTLASLKHDQEQPSTFSMPLLMGSVLSCGKSSPGKLHLMKEVICIPSPGLTAKQSVELLLEIQKQVNRAMETLPPPKQETKKGHNGSKRAQPPITGKVSHLGCLTINYDAIEQPLLLLQGICSNLGLELGVNFHLAINCAGHELMDYSKGKYEVMVGTHKSAAEMVELYVDLINKYPSIIALIDPFRKEDAEQWDSLYAALASRCYLIAGAASGSVSKLLECRNISTLKSHGLIIKHTNQTTMSDLVEITHLINGKKLLAVFGSTDSESSDDSLVDLRMLLPQWLRKLLGSWTPSSPQR

Target ORF information:

RefSeq Version	XM_006527056.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus enolase 4 (Eno4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527056.4

ORF Insert Sequence:

ATGGGAGATG AAGACGGCGG CCGCAGAGGC GGGATCACTA GGGACCTGCA GAAGCTGAAG 
CAGCAGGCGA TGGCGTACTA CCAGGAGAAC GACGTCCCGC GCAAGCTGGA AGATCTGCTC 
AACTCCACCT TCTACCTCCA GCCTGCGGAT GTCTACGGGC ACCTGGCAAA CTACTTTTCT 
AAACTTGCGA AGCCTCCCTC CATATGCAAA ATAGTAGGGA AAACCATACT GGATGGCCTG 
GGACTTCCTA CTCTACAGGT GGAAATCTCC TGCACCATTC AAAATTTCCC CAAGTACATC 
TGTGCCGTGG CGATCCCTAC GCACTTTGAA GTCGTGGAGA ACGCTTTGCC AGAGGCGTTG 
GATGCAGAGG ACTCAGAAAG GGCCCAGGCC GTGAACACAG CGGTGCAGTG GATCAATCAG 
TCCATCACAG AGGAGCTGTG GGGTCTGGTC CCCTCCAACC AGGCAGAGGT GGACCACAGG 
CTCAGGACCT TCTTTGAACA TAAAGTACAA GAAGATAAAG AGAGAAAAGA ATTGGAAAAG 
AGCCAGGAGG AGCTGGTTCC CGCACCTCCA CCAGTAACGC TGCCGCCGCC GCCGCCGCCG 
CCTCCCCCAC CTCCCTCCAA AAAGAAAGGG CAGAAGGCAG GACGGAGGGA TACGCTTTTG 
GAGAAACCTG TTTCACCCCC GGAGCCCCCT GAACCAGTCC TCCATGGTAG CATGGCCATA 
GGCGCTGTGT CCCTGGCAGT GGCCAAAGCC AGCGCAACCT TGGCCAGCGA TCCTCTCTAC 
TTAACCCTGG CGTCACTGAA GCATGATCAG GAGCAGCCGT CAACATTCTC TATGCCTTTG 
CTGATGGGAT CTGTACTGAG CTGTGGGAAG TCGTCACCTG GGAAGCTACA TTTAATGAAA 
GAAGTGATTT GTATTCCCAG TCCTGGATTA ACAGCCAAAC AAAGTGTCGA GTTGCTTCTG 
GAAATTCAGA AACAAGTTAA CAGAGCGATG GAGACGCTTC CACCTCCAAA ACAAGAGACC 
AAAAAAGGGC ATAATGGGAG CAAAAGAGCT CAGCCACCGA TCACTGGCAA GGTATCTCAC 
CTTGGCTGTT TAACCATCAA CTACGACGCC ATAGAGCAGC CACTGCTGCT GCTTCAGGGG 
ATCTGCTCAA ACCTGGGGTT GGAACTGGGA GTGAACTTCC ATCTAGCCAT CAACTGTGCT 
GGGCATGAGC TGATGGACTA CAGTAAAGGG AAGTACGAAG TGATGGTGGG CACCCACAAA 
AGCGCAGCTG AGATGGTGGA GCTCTACGTG GACCTGATCA ACAAGTATCC TTCCATAATC 
GCCTTAATTG ATCCTTTCAG GAAGGAGGAC GCCGAGCAGT GGGACAGCCT CTATGCTGCT 
CTGGCTTCCC GGTGCTACCT AATTGCAGGC GCCGCTTCCG GGAGTGTCTC TAAACTCCTA 
GAGTGCAGAA ATATAAGCAC CCTGAAATCC CACGGACTGA TCATAAAGCA CACAAACCAA 
ACCACAATGT CTGACTTGGT GGAAATAACC CATCTTATCA ACGGTAAGAA GCTCCTGGCC 
GTCTTTGGAA GCACAGACTC GGAGTCCTCT GATGACAGCC TTGTCGATTT GAGGATGCTA 
CTGCCACAAT GGCTGAGGAA ACTCTTGGGC TCCTGGACTC CATCTTCCCC ACAGAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Disruption of a spermatogenic cell-specific mouse enolase 4 (eno4) gene causes sperm structural defects and male infertility.
Biology of reproduction88(4)90(2013 Apr)
Nakamura N,Dai Q,Williams J,Goulding EH,Willis WD,Brown PR,Eddy EM

Integrated microarray and ChIP analysis identifies multiple Foxa2 dependent target genes in the notochord.
Developmental biology360(2)415-25(2011 Dec)
Tamplin OJ,Cox BJ,Rossant J

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

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Eno4 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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