Siae cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Siae
Entrez Gene ID	22619
Full Name	sialic acid acetylesterase
Synonyms	LSE,Ysg2
General protein information	Preferred Names sialic acid acetylesterase Names sialate O-acetylesterase clone 165 sialic acid-specific 9-O-acetylesterase yolk sac gene 2 yolk sac protein 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9 A4\|9 20.79 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_011734.4	NP_035864.2	sialate O-acetylesterase precursor
NM_011734.3	NP_035864.2	sialate O-acetylesterase precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SIAE	XP_858869.2
Danio rerio (zebrafish)	siae	NP_001037796.1
Homo sapiens (human)	SIAE	NP_733746.1
Pan troglodytes (chimpanzee)	SIAE	XP_508836.2
Rattus norvegicus (Norway rat)	Siae	NP_001102229.1
	SIAE	XP_001109153.1
Bos taurus (cattle)	SIAE	NP_001039433.1
Mus musculus (house mouse)	Siae	NP_035864.2
Xenopus tropicalis (tropical clawed frog)	LOC100493404	XP_002940054.1

Gene Ontology
Bibliography

Related articles in PubMed

Striking Immune Phenotypes in Gene-Targeted Mice Are Driven by a Copy-Number Variant Originating from a Commercially Available C57BL/6 Strain.
Mahajan VS, Demissie E, Mattoo H, Viswanadham V, Varki A, Morris R, Pillai S
Cell reports15(9)1901-9(2016 05)

B cell antigen receptor signal strength and peripheral B cell development are regulated by a 9-O-acetyl sialic acid esterase.
Cariappa A, Takematsu H, Liu H, Diaz S, Haider K, Boboila C, Kalloo G, Connole M, Shi HN, Varki N, Varki A, Pillai S
The Journal of experimental medicine206(1)125-38(2009 Jan)

Construction of long-transcript enriched cDNA libraries from submicrogram amounts of total RNAs by a universal PCR amplification method.
Piao Y, Ko NT, Lim MK, Ko MS
Genome research11(9)1553-8(2001 Sep)

Lysosomal and cytosolic sialic acid 9-O-acetylesterase activities can Be encoded by one gene via differential usage of a signal peptide-encoding exon at the N terminus.
Takematsu H, Diaz S, Stoddart A, Zhang Y, Varki A
The Journal of biological chemistry274(36)25623-31(1999 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Sialate:O-acetyl esterase is a catalytic regulator of B cell signaling and plays an essential role in the maintenance of immunological tolerance in the B lineage.
Title: B cell antigen receptor signal strength and peripheral B cell development are regulated by a 9-O-acetyl sialic acid esterase.

The following Siae gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Siae cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu08715	NM_011734.4 Latest version!	Mus musculus sialic acid acetylesterase (Siae), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
View Old Accession Versions >>
OMu08715	NM_011734.3	Mus musculus sialic acid acetylesterase (Siae), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Siae ( NM_011734.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035864.2 Mus musculus sialic acid acetylesterase (Siae), mRNA.

Price & Availability↑

CloneID	OMu08715
Clone ID Related Accession (Same CDS sequence)	NM_011734.4 , NM_011734.3
Accession Version	NM_011734.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1626bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Mus musculus(house mouse)
Product	sialate O-acetylesterase precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ062379.1 and AK028656.1. On Jun 19, 2018 this sequence version replaced NM_011734.3. ##Evidence-Data-START## Transcript exon combination :: SRR1660821.34224.1, AK165103.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTTTCCC CGGGGCCTGT GTTTGGGATA GTGCTGCTCA TAATCGCGCG AGTCAGCAGA 
AGTGCAGGTA TTGGTTTTCG CTTTGCTTCA TACATCGATA ATTACATGGT GCTGCAGAAG 
GAGCCTTCAG GGGCTGTGAT CTGGGGCTTT GGTACACCTG GAGCCACAGT GACAGTGACC 
TTGTGCCAAG GTCAGGAAAC CATCATGAAG AAAGTGACCA GTGTGAAAGA ACCCTCCAAC 
ACCTGGATGG TGGTACTGGA CCCTATGAAG CCTGGGGGAC CTTTTGAAGT GATGGCGCAA 
CAGACTTTGG GGACAATGAA TTTCACCCTG AGAGTCCATG ATGTCTTATT TGGAGATGTC 
TGGCTTTGCA GTGGGCAGAG TAACATGCAG ATGACTGTTT CACAGATCTT TAACGCCTCA 
AAGGAGTTGT CCGACACTGC TGCCTACCAG TCTGTGCGCA TCTTCTCTGT CTCCCTCATC 
CAATCAGAGG AGGAGTTGGA TGACCTTACT GAGGTTGACC TGTCATGGTC CAAGCCCACC 
GCAGGAAACT TGGGCCATGG AAATTTCACT TACATGTCTG CGGTGTGCTG GCTCTTTGGG 
CGTTACCTGT ATGACACTCT GCAATATCCC ATTGGGTTGG TCTCCTCCAG TTGGGGTGGG 
ACGTACATTG AAGTCTGGTC ATCTAGAAGG ACACTGAAAG CCTGTGGAGT CCCTAATACA 
AGGGATGAAA GAGTTGGTCA GCCCGAGATA AAGCCCATGA GGAATGAATG TAACAGTGAG 
GAGTCCTCAT GTCCTTTTAG GGTTGTCCCA TCTGTTCGTG TGACTGGACC GACAAGACAC 
TCCGTCCTTT GGAACGCCAT GATCCATCCA CTGCAAAACA TGACTTTGAA AGGGGTGGTG 
TGGTACCAGG GAGAGTCCAA TGCGGATTAT AACAGGGACC TGTACACCTG CATGTTTCCT 
GAACTCATTG AAGACTGGCG CCAGACCTTC CACTACGGCT CCCAGGGTCA GACAGATCGT 
TTCTTTCCAT TTGGGTTTGT CCAGCTGTCT TCATACATGT TAAAGAACTC ATCGGATTAT 
GGATTTCCAG AGATCCGTTG GCATCAAACT GCAGACTTTG GCCATGTCCC CAACCCGAAG 
ATGCCTAATA CTTTTATGGC TGTCGCTATT GACCTCTGTG ATAGAGACTC ACCCTTTGGA 
AGTATCCACC CTCGAGACAA ACAGACTGTG GCCTATCGTC TGCACTTGGG TGCTCGAGCT 
GTGGCATATG GTGAGAAAAA TTTGACCTTT CAAGGACCAC TACCTAAGAA GATAGAACTC 
TTGGCTTCCA ATGGCCTGCT CAACCTCACA TATGACCAGG AAATCCAGGT GCAAATGCAA 
GATAACAAAA CATTTGAGAT CTCCTGTTGT AGTGACCGTC ATTGCAAATG GCTTCCAGCT 
CCTGTTAACA CTTTCTCCAC CCAGACTCTG ATTTTGGATC TCAATGCTTG TCTTGGCACA 
GTGGTTGCTG TTCGCTATGC CTGGACCACA TGGCCTTGTG AATATAAGCA ATGTGCTGTT 
TACCACACCA GCAGTATGTT GCCAGCTCCT CCCTTCATTG CTCAAATTTC ACACAGGGGT 
ATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035864.2
CDS	40..1665
Translation	MVSPGPVFGIVLLIIARVSRSAGIGFRFASYIDNYMVLQKEPSGAVIWGFGTPGATVTVTLCQGQETIMKKVTSVKEPSNTWMVVLDPMKPGGPFEVMAQQTLGTMNFTLRVHDVLFGDVWLCSGQSNMQMTVSQIFNASKELSDTAAYQSVRIFSVSLIQSEEELDDLTEVDLSWSKPTAGNLGHGNFTYMSAVCWLFGRYLYDTLQYPIGLVSSSWGGTYIEVWSSRRTLKACGVPNTRDERVGQPEIKPMRNECNSEESSCPFRVVPSVRVTGPTRHSVLWNAMIHPLQNMTLKGVVWYQGESNADYNRDLYTCMFPELIEDWRQTFHYGSQGQTDRFFPFGFVQLSSYMLKNSSDYGFPEIRWHQTADFGHVPNPKMPNTFMAVAIDLCDRDSPFGSIHPRDKQTVAYRLHLGARAVAYGEKNLTFQGPLPKKIELLASNGLLNLTYDQEIQVQMQDNKTFEISCCSDRHCKWLPAPVNTFSTQTLILDLNACLGTVVAVRYAWTTWPCEYKQCAVYHTSSMLPAPPFIAQISHRGI

Target ORF information:

RefSeq Version	NM_011734.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus sialic acid acetylesterase (Siae), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011734.4

ORF Insert Sequence:

ATGGTTTCCC CGGGGCCTGT GTTTGGGATA GTGCTGCTCA TAATCGCGCG AGTCAGCAGA 
AGTGCAGGTA TTGGTTTTCG CTTTGCTTCA TACATCGATA ATTACATGGT GCTGCAGAAG 
GAGCCTTCAG GGGCTGTGAT CTGGGGCTTT GGTACACCTG GAGCCACAGT GACAGTGACC 
TTGTGCCAAG GTCAGGAAAC CATCATGAAG AAAGTGACCA GTGTGAAAGA ACCCTCCAAC 
ACCTGGATGG TGGTACTGGA CCCTATGAAG CCTGGGGGAC CTTTTGAAGT GATGGCGCAA 
CAGACTTTGG GGACAATGAA TTTCACCCTG AGAGTCCATG ATGTCTTATT TGGAGATGTC 
TGGCTTTGCA GTGGGCAGAG TAACATGCAG ATGACTGTTT CACAGATCTT TAACGCCTCA 
AAGGAGTTGT CCGACACTGC TGCCTACCAG TCTGTGCGCA TCTTCTCTGT CTCCCTCATC 
CAATCAGAGG AGGAGTTGGA TGACCTTACT GAGGTTGACC TGTCATGGTC CAAGCCCACC 
GCAGGAAACT TGGGCCATGG AAATTTCACT TACATGTCTG CGGTGTGCTG GCTCTTTGGG 
CGTTACCTGT ATGACACTCT GCAATATCCC ATTGGGTTGG TCTCCTCCAG TTGGGGTGGG 
ACGTACATTG AAGTCTGGTC ATCTAGAAGG ACACTGAAAG CCTGTGGAGT CCCTAATACA 
AGGGATGAAA GAGTTGGTCA GCCCGAGATA AAGCCCATGA GGAATGAATG TAACAGTGAG 
GAGTCCTCAT GTCCTTTTAG GGTTGTCCCA TCTGTTCGTG TGACTGGACC GACAAGACAC 
TCCGTCCTTT GGAACGCCAT GATCCATCCA CTGCAAAACA TGACTTTGAA AGGGGTGGTG 
TGGTACCAGG GAGAGTCCAA TGCGGATTAT AACAGGGACC TGTACACCTG CATGTTTCCT 
GAACTCATTG AAGACTGGCG CCAGACCTTC CACTACGGCT CCCAGGGTCA GACAGATCGT 
TTCTTTCCAT TTGGGTTTGT CCAGCTGTCT TCATACATGT TAAAGAACTC ATCGGATTAT 
GGATTTCCAG AGATCCGTTG GCATCAAACT GCAGACTTTG GCCATGTCCC CAACCCGAAG 
ATGCCTAATA CTTTTATGGC TGTCGCTATT GACCTCTGTG ATAGAGACTC ACCCTTTGGA 
AGTATCCACC CTCGAGACAA ACAGACTGTG GCCTATCGTC TGCACTTGGG TGCTCGAGCT 
GTGGCATATG GTGAGAAAAA TTTGACCTTT CAAGGACCAC TACCTAAGAA GATAGAACTC 
TTGGCTTCCA ATGGCCTGCT CAACCTCACA TATGACCAGG AAATCCAGGT GCAAATGCAA 
GATAACAAAA CATTTGAGAT CTCCTGTTGT AGTGACCGTC ATTGCAAATG GCTTCCAGCT 
CCTGTTAACA CTTTCTCCAC CCAGACTCTG ATTTTGGATC TCAATGCTTG TCTTGGCACA 
GTGGTTGCTG TTCGCTATGC CTGGACCACA TGGCCTTGTG AATATAAGCA ATGTGCTGTT 
TACCACACCA GCAGTATGTT GCCAGCTCCT CCCTTCATTG CTCAAATTTC ACACAGGGGT 
ATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Siae ( NM_011734.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035864.2 Mus musculus sialic acid acetylesterase (Siae), mRNA.

Price & Availability↑

CloneID	OMu08715
Clone ID Related Accession (Same CDS sequence)	NM_011734.4 , NM_011734.3
Accession Version	NM_011734.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1626bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-01
Organism	Mus musculus(house mouse)
Product	sialate O-acetylesterase precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK028656.1. On Apr 5, 2007 this sequence version replaced NM_011734.2. ##Evidence-Data-START## Transcript exon combination :: AK028656.1, SRR1660821.34224.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGTTTCCC CGGGGCCTGT GTTTGGGATA GTGCTGCTCA TAATCGCGCG AGTCAGCAGA 
AGTGCAGGTA TTGGTTTTCG CTTTGCTTCA TACATCGATA ATTACATGGT GCTGCAGAAG 
GAGCCTTCAG GGGCTGTGAT CTGGGGCTTT GGTACACCTG GAGCCACAGT GACAGTGACC 
TTGTGCCAAG GTCAGGAAAC CATCATGAAG AAAGTGACCA GTGTGAAAGA ACCCTCCAAC 
ACCTGGATGG TGGTACTGGA CCCTATGAAG CCTGGGGGAC CTTTTGAAGT GATGGCGCAA 
CAGACTTTGG GGACAATGAA TTTCACCCTG AGAGTCCATG ATGTCTTATT TGGAGATGTC 
TGGCTTTGCA GTGGGCAGAG TAACATGCAG ATGACTGTTT CACAGATCTT TAACGCCTCA 
AAGGAGTTGT CCGACACTGC TGCCTACCAG TCTGTGCGCA TCTTCTCTGT CTCCCTCATC 
CAATCAGAGG AGGAGTTGGA TGACCTTACT GAGGTTGACC TGTCATGGTC CAAGCCCACC 
GCAGGAAACT TGGGCCATGG AAATTTCACT TACATGTCTG CGGTGTGCTG GCTCTTTGGG 
CGTTACCTGT ATGACACTCT GCAATATCCC ATTGGGTTGG TCTCCTCCAG TTGGGGTGGG 
ACGTACATTG AAGTCTGGTC ATCTAGAAGG ACACTGAAAG CCTGTGGAGT CCCTAATACA 
AGGGATGAAA GAGTTGGTCA GCCCGAGATA AAGCCCATGA GGAATGAATG TAACAGTGAG 
GAGTCCTCAT GTCCTTTTAG GGTTGTCCCA TCTGTTCGTG TGACTGGACC GACAAGACAC 
TCCGTCCTTT GGAACGCCAT GATCCATCCA CTGCAAAACA TGACTTTGAA AGGGGTGGTG 
TGGTACCAGG GAGAGTCCAA TGCGGATTAT AACAGGGACC TGTACACCTG CATGTTTCCT 
GAACTCATTG AAGACTGGCG CCAGACCTTC CACTACGGCT CCCAGGGTCA GACAGATCGT 
TTCTTTCCAT TTGGGTTTGT CCAGCTGTCT TCATACATGT TAAAGAACTC ATCGGATTAT 
GGATTTCCAG AGATCCGTTG GCATCAAACT GCAGACTTTG GCCATGTCCC CAACCCGAAG 
ATGCCTAATA CTTTTATGGC TGTCGCTATT GACCTCTGTG ATAGAGACTC ACCCTTTGGA 
AGTATCCACC CTCGAGACAA ACAGACTGTG GCCTATCGTC TGCACTTGGG TGCTCGAGCT 
GTGGCATATG GTGAGAAAAA TTTGACCTTT CAAGGACCAC TACCTAAGAA GATAGAACTC 
TTGGCTTCCA ATGGCCTGCT CAACCTCACA TATGACCAGG AAATCCAGGT GCAAATGCAA 
GATAACAAAA CATTTGAGAT CTCCTGTTGT AGTGACCGTC ATTGCAAATG GCTTCCAGCT 
CCTGTTAACA CTTTCTCCAC CCAGACTCTG ATTTTGGATC TCAATGCTTG TCTTGGCACA 
GTGGTTGCTG TTCGCTATGC CTGGACCACA TGGCCTTGTG AATATAAGCA ATGTGCTGTT 
TACCACACCA GCAGTATGTT GCCAGCTCCT CCCTTCATTG CTCAAATTTC ACACAGGGGT 
ATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035864.2
CDS	14..1639
Translation	MVSPGPVFGIVLLIIARVSRSAGIGFRFASYIDNYMVLQKEPSGAVIWGFGTPGATVTVTLCQGQETIMKKVTSVKEPSNTWMVVLDPMKPGGPFEVMAQQTLGTMNFTLRVHDVLFGDVWLCSGQSNMQMTVSQIFNASKELSDTAAYQSVRIFSVSLIQSEEELDDLTEVDLSWSKPTAGNLGHGNFTYMSAVCWLFGRYLYDTLQYPIGLVSSSWGGTYIEVWSSRRTLKACGVPNTRDERVGQPEIKPMRNECNSEESSCPFRVVPSVRVTGPTRHSVLWNAMIHPLQNMTLKGVVWYQGESNADYNRDLYTCMFPELIEDWRQTFHYGSQGQTDRFFPFGFVQLSSYMLKNSSDYGFPEIRWHQTADFGHVPNPKMPNTFMAVAIDLCDRDSPFGSIHPRDKQTVAYRLHLGARAVAYGEKNLTFQGPLPKKIELLASNGLLNLTYDQEIQVQMQDNKTFEISCCSDRHCKWLPAPVNTFSTQTLILDLNACLGTVVAVRYAWTTWPCEYKQCAVYHTSSMLPAPPFIAQISHRGI

Target ORF information:

RefSeq Version	NM_011734.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus sialic acid acetylesterase (Siae), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011734.3

ORF Insert Sequence:

ATGGTTTCCC CGGGGCCTGT GTTTGGGATA GTGCTGCTCA TAATCGCGCG AGTCAGCAGA 
AGTGCAGGTA TTGGTTTTCG CTTTGCTTCA TACATCGATA ATTACATGGT GCTGCAGAAG 
GAGCCTTCAG GGGCTGTGAT CTGGGGCTTT GGTACACCTG GAGCCACAGT GACAGTGACC 
TTGTGCCAAG GTCAGGAAAC CATCATGAAG AAAGTGACCA GTGTGAAAGA ACCCTCCAAC 
ACCTGGATGG TGGTACTGGA CCCTATGAAG CCTGGGGGAC CTTTTGAAGT GATGGCGCAA 
CAGACTTTGG GGACAATGAA TTTCACCCTG AGAGTCCATG ATGTCTTATT TGGAGATGTC 
TGGCTTTGCA GTGGGCAGAG TAACATGCAG ATGACTGTTT CACAGATCTT TAACGCCTCA 
AAGGAGTTGT CCGACACTGC TGCCTACCAG TCTGTGCGCA TCTTCTCTGT CTCCCTCATC 
CAATCAGAGG AGGAGTTGGA TGACCTTACT GAGGTTGACC TGTCATGGTC CAAGCCCACC 
GCAGGAAACT TGGGCCATGG AAATTTCACT TACATGTCTG CGGTGTGCTG GCTCTTTGGG 
CGTTACCTGT ATGACACTCT GCAATATCCC ATTGGGTTGG TCTCCTCCAG TTGGGGTGGG 
ACGTACATTG AAGTCTGGTC ATCTAGAAGG ACACTGAAAG CCTGTGGAGT CCCTAATACA 
AGGGATGAAA GAGTTGGTCA GCCCGAGATA AAGCCCATGA GGAATGAATG TAACAGTGAG 
GAGTCCTCAT GTCCTTTTAG GGTTGTCCCA TCTGTTCGTG TGACTGGACC GACAAGACAC 
TCCGTCCTTT GGAACGCCAT GATCCATCCA CTGCAAAACA TGACTTTGAA AGGGGTGGTG 
TGGTACCAGG GAGAGTCCAA TGCGGATTAT AACAGGGACC TGTACACCTG CATGTTTCCT 
GAACTCATTG AAGACTGGCG CCAGACCTTC CACTACGGCT CCCAGGGTCA GACAGATCGT 
TTCTTTCCAT TTGGGTTTGT CCAGCTGTCT TCATACATGT TAAAGAACTC ATCGGATTAT 
GGATTTCCAG AGATCCGTTG GCATCAAACT GCAGACTTTG GCCATGTCCC CAACCCGAAG 
ATGCCTAATA CTTTTATGGC TGTCGCTATT GACCTCTGTG ATAGAGACTC ACCCTTTGGA 
AGTATCCACC CTCGAGACAA ACAGACTGTG GCCTATCGTC TGCACTTGGG TGCTCGAGCT 
GTGGCATATG GTGAGAAAAA TTTGACCTTT CAAGGACCAC TACCTAAGAA GATAGAACTC 
TTGGCTTCCA ATGGCCTGCT CAACCTCACA TATGACCAGG AAATCCAGGT GCAAATGCAA 
GATAACAAAA CATTTGAGAT CTCCTGTTGT AGTGACCGTC ATTGCAAATG GCTTCCAGCT 
CCTGTTAACA CTTTCTCCAC CCAGACTCTG ATTTTGGATC TCAATGCTTG TCTTGGCACA 
GTGGTTGCTG TTCGCTATGC CTGGACCACA TGGCCTTGTG AATATAAGCA ATGTGCTGTT 
TACCACACCA GCAGTATGTT GCCAGCTCCT CCCTTCATTG CTCAAATTTC ACACAGGGGT 
ATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Striking Immune Phenotypes in Gene-Targeted Mice Are Driven by a Copy-Number Variant Originating from a Commercially Available C57BL/6 Strain.
Cell reports15(9)1901-9(2016 05)
Mahajan VS,Demissie E,Mattoo H,Viswanadham V,Varki A,Morris R,Pillai S

B cell antigen receptor signal strength and peripheral B cell development are regulated by a 9-O-acetyl sialic acid esterase.
The Journal of experimental medicine206(1)125-38(2009 Jan)
Cariappa A,Takematsu H,Liu H,Diaz S,Haider K,Boboila C,Kalloo G,Connole M,Shi HN,Varki N,Varki A,Pillai S

Construction of long-transcript enriched cDNA libraries from submicrogram amounts of total RNAs by a universal PCR amplification method.
Genome research11(9)1553-8(2001 Sep)
Piao Y,Ko NT,Lim MK,Ko MS

Lysosomal and cytosolic sialic acid 9-O-acetylesterase activities can Be encoded by one gene via differential usage of a signal peptide-encoding exon at the N terminus.
The Journal of biological chemistry274(36)25623-31(1999 Sep)
Takematsu H,Diaz S,Stoddart A,Zhang Y,Varki A

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Siae cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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