Kdm2a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Kdm2a
Entrez Gene ID	225876
Full Name	lysine (K)-specific demethylase 2A
Synonyms	100043628,5530401A10Rik,AA589516,AW536790,Cxxc8,Fbl11,Fbl7,Fbxl11,Gm4560,Jhdm1,Jhdm1a,lalina
General protein information	Preferred Names lysine (K)-specific demethylase 2A Names lysine-specific demethylase 2A F-box and leucine-rich repeat protein 11 F-box protein containing leucine repeats F-box/LRR-repeat protein 11 [Histone-H3]-lysine-36 demethylase 1A jmjC domain-containing histone demethylation protein 1A
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 19 Map Location: 19\|19 A

mRNA and Protein(s)

mRNA	Protein	Name
XM_006531722.4	XP_006531785.1	lysine-specific demethylase 2A isoform X1
NM_001001984.2	NP_001001984.2	lysine-specific demethylase 2A
XM_006531722.3	XP_006531785.1	lysine-specific demethylase 2A isoform X1
XM_006531722.2	XP_006531785.1	lysine-specific demethylase 2A isoform X1
XM_006531723.3	XP_006531786.1	lysine-specific demethylase 2A isoform X2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	KDM2A	XP_865102.2
Bos taurus (cattle)	KDM2A	XP_002699409.2
Mus musculus (house mouse)	Kdm2a	NP_001001984.2
Rattus norvegicus (Norway rat)	Kdm2a	NP_001101985.1
Homo sapiens (human)	KDM2A	NP_036440.1
Danio rerio (zebrafish)	kdm2aa	NP_001075161.2
	KDM2A	XP_001107511.1
Gallus gallus (chicken)	KDM2A	XP_423678.3
Pan troglodytes (chimpanzee)	KDM2A	XP_001172828.1
Danio rerio (zebrafish)	kdm2a	XP_005173200.1
Drosophila melanogaster (fruit fly)	Kdm2	NP_001262400.1
Xenopus tropicalis (tropical clawed frog)	kdm2a	NP_001011176.1

Gene Ontology
Bibliography

Related articles in PubMed

Broad histone H3K4me3 domains in mouse oocytes modulate maternal-to-zygotic transition.
Dahl JA, Jung I, Aanes H, Greggains GD, Manaf A, Lerdrup M, Li G, Kuan S, Li B, Lee AY, Preissl S, Jermstad I, Haugen MH, Suganthan R, Bj?r?s M, Hansen K, Dalen KT, Fedorcsak P, Ren B, Klungland A
Nature537(7621)548-552(2016 09)

Spatial and temporal expression of histone demethylase, Kdm2a, during murine molar development.
Yi Q, Cao Y, Liu OS, Lu YQ, Wang JS, Wang SL, Yao R, Fan ZP
Biotechnic & histochemistry : official publication of the Biological Stain Commission91(2)137-44(2016)

The histone demethylase Fbxl11/Kdm2a plays an essential role in embryonic development by repressing cell-cycle regulators.
Kawakami E, Tokunaga A, Ozawa M, Sakamoto R, Yoshida N
Mechanisms of development13531-42(2015 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Kdm2a expression may be closely related to cell proliferation
Title: Spatial and temporal expression of histone demethylase, Kdm2a, during murine molar development.

Fbxl11 plays an essential role in embryonic development and homeostasis by regulating cell proliferation and survival.
Title: The histone demethylase Fbxl11/Kdm2a plays an essential role in embryonic development by repressing cell-cycle regulators.

we identify Jhdm1a negatively regulates gluconeogenic gene PEPCK and G6Pase expression both in vitro and in vivo
Title: The histone demethylase Jhdm1a regulates hepatic gluconeogenesis.

Data describe a NF-kappaB regulatory pathway that is driven by reversible lysine methylation of p65, carried out by nuclear receptor-binding SET domain-containing protein 1 (NSD1) and F-box and leucine-rich repeat protein 11 (FBXL11).
Title: Regulation of NF-kappaB by NSD1/FBXL11-dependent reversible lysine methylation of p65.

The following Kdm2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Kdm2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu55050	XM_006531723.3 Latest version!	Mus musculus lysine (K)-specific demethylase 2A (Kdm2a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu10441	NM_001001984.2 Latest version!	Mus musculus lysine (K)-specific demethylase 2A (Kdm2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
OMu10441	XM_006531722.4 Latest version!	Mus musculus lysine (K)-specific demethylase 2A (Kdm2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OMu10441	XM_006531722.3	Mus musculus lysine (K)-specific demethylase 2A (Kdm2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
OMu10441	XM_006531722.2	Mus musculus lysine (K)-specific demethylase 2A (Kdm2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Kdm2a ( XM_006531723.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006531786.1 Mus musculus lysine (K)-specific demethylase 2A (Kdm2a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu55050
Clone ID Related Accession (Same CDS sequence)	XM_006531723.3
Accession Version	XM_006531723.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	lysine-specific demethylase 2A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006531723.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTGGG AGATTCGGGA TTTTCACGAA AATCAGGATT TTCCTAGAAG CAGAAAAGCT 
AACTCCTTCC GCACAACTTC AACACAGAAA ACAAAACAAA GGCGCTGGGG AAACTTCACC 
CAAGAGCCTC CGCAGCTCCA GAGCAACAAG AAATGCAAGA TTTTGCTGGA AGAACTTGCC 
AGTAGCGATC CCAAGTTAGC CCTCACTGGA GTCCCTATAG TACAGTGGCC AAAAAGGGAT 
AAGCTTAAAT TCCCTACCAG GCCAAAGGTG AGGGTTCCTA CAATTCCCAT CACAAAGCCT 
CACACCATGA AGCCAGCTCC ACGCTTAACA CCTGTAAGGC CTGCTGCAGC CTCCCCCATT 
GTGTCAGGAG CCAGGCGGAG AAGAGTGCGG TGCAGGAAAT GCAAAGCTTG TGTGCAAGGA 
GAATGTGGAG TCTGCCACTA CTGCAGGGAC ATGAAGAAAT TTGGTGGACC TGGACGCATG 
AAGCAATCCT GTGTCCTCCG ACAGTGCTTA GCACCCAGAC TGCCTCATTC AGTTACGTGT 
TCTCTCTGTG GAGAAGTAGA TCAGAATGAA GAGACCCAGG ACTTTGAAAA GAAACTCATG 
GAATGCTGCA TCTGCAACGA GATAGTTCAT CCTGGCTGCC TCCAGATGGA TGGAGAGGGG 
TTGCTGAACG AGGAATTGCC AAATTGCTGG GAGTGTCCAA AGTGTTACCA GGAAGACAGC 
TCGGACAAAG CCCAGAAGCG GAAAATAGAA GAGAGTGATG AAGAAGCTGT ACAAGCCAAA 
GTCTTACGGC CCCTGAGGAG CTGCGAGGAG CCTCTCACAC CCCCGCCTCA CTCACCTACT 
TCCATGCTGC AGCTCATCCA CGACCCGGTT TCTCCCCGGG GTATGGTGAC TCGGTCATCC 
CCTGGGGCTG GCCCCAGCGA CCACCACAGT GCCAGCCGTG ATGAACGCTT CAAACGGCGG 
CAGTTGCTGC GGCTACAAGC CACCGAGCGC ACCATGGTAC GGGAAAAGGA GAACAATCCC 
AGCGGCAAAA AGGAGCTGTC TGAAGTTGAG AAAGCCAAGA TCCGGGGATC GTACCTCACT 
GTCACTCTAC AGAGGCCCAC CAAAGAGCTC CACGGGACAT CCATTGTGCC CAAGCTGCAG 
GCCATCACGG CCTCCTCTGC CAACCTTCGC CCTAACCCCC GCGTGCTAAT GCAGCACTGC 
CCAGCCCGAA ACCCCCAGCA TGGGGATGAG GAGGGGCTTG GGGGAGAGGA GGAGGAAGAG 
GAGGAGGAGG AGGAAGATGA CAGTGCAGAG GAGGGGGGTG CAGCCAGGCT GAATGGCCGG 
GGCAGTTGGG CTCAGGATGG AGACGAAAGC TGGATGCAGC GGGAGGTCTG GATGTCTGTC 
TTCCGCTACC TCAGCCGCAA AGAACTTTGT GAATGTATGC GAGTGTGCAA GACATGGTAT 
AAATGGTGCT GTGATAAACG ACTTTGGACA AAAATTGACT TGAGTAGGTG TAAGGCCATC 
GTACCACAAG CTCTCAGTGG TATCATCAAG CGGCAGCCAG TAAGCCTCGA CCTCAGCTGG 
ACTAACATCT CCAAAAAGCA GCTGACATGG CTGGTCAATA GGCTGCCAGG ATTAAAAGAC 
CTCCTCCTAG CAGGCTGTTC CTGGTCTGCA GTATCTGCCC TCAGCACTTC CAGCTGCCCG 
CTTCTCAGGA CCCTTGATCT TCGGTGGGCA GTAGGAATTA AAGACCCTCA AATTCGGGAC 
TTGCTGACTC CACCCACAGA TAAGCCAGGT CAAGACAATC GAAGCAAACT CCGGAACATG 
ACTGACTTCC GGCTGGCAGG CCTTGACATC ACAGATGCTA CTCTCCGACT CATCATTCGC 
CACATGCCCC TTTTGTCTCG ACTTGACCTC AGTCACTGCA GTCACCTTAC AGATCAGTCC 
TCCAACCTAC TAACTGCTGT CGGGTCTTCC ACTCGATACT CCCTTACAGA GCTCAATATG 
GCAGGTTGCA ATAAATTGAC AGACCAGACC CTGTTCTTCC TAAGGCGAAT TGCTAATGTC 
ACCTTGATTG ACCTTCGAGG ATGCAAACAG ATCACGAGAA AAGCCTGTGA GCACTTCATC 
TCAGACTTGT CCATCAACAG CCTCTACTGC CTGTCTGATG AGAAACTGAT ACAGAAGATT 
AGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006531786.1
CDS	339..2504
Translation	MLWEIRDFHENQDFPRSRKANSFRTTSTQKTKQRRWGNFTQEPPQLQSNKKCKILLEELASSDPKLALTGVPIVQWPKRDKLKFPTRPKVRVPTIPITKPHTMKPAPRLTPVRPAAASPIVSGARRRRVRCRKCKACVQGECGVCHYCRDMKKFGGPGRMKQSCVLRQCLAPRLPHSVTCSLCGEVDQNEETQDFEKKLMECCICNEIVHPGCLQMDGEGLLNEELPNCWECPKCYQEDSSDKAQKRKIEESDEEAVQAKVLRPLRSCEEPLTPPPHSPTSMLQLIHDPVSPRGMVTRSSPGAGPSDHHSASRDERFKRRQLLRLQATERTMVREKENNPSGKKELSEVEKAKIRGSYLTVTLQRPTKELHGTSIVPKLQAITASSANLRPNPRVLMQHCPARNPQHGDEEGLGGEEEEEEEEEEDDSAEEGGAARLNGRGSWAQDGDESWMQREVWMSVFRYLSRKELCECMRVCKTWYKWCCDKRLWTKIDLSRCKAIVPQALSGIIKRQPVSLDLSWTNISKKQLTWLVNRLPGLKDLLLAGCSWSAVSALSTSSCPLLRTLDLRWAVGIKDPQIRDLLTPPTDKPGQDNRSKLRNMTDFRLAGLDITDATLRLIIRHMPLLSRLDLSHCSHLTDQSSNLLTAVGSSTRYSLTELNMAGCNKLTDQTLFFLRRIANVTLIDLRGCKQITRKACEHFISDLSINSLYCLSDEKLIQKIS

Target ORF information:

RefSeq Version	XM_006531723.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus lysine (K)-specific demethylase 2A (Kdm2a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006531723.3

ORF Insert Sequence:

ATGCTCTGGG AGATTCGGGA TTTTCACGAA AATCAGGATT TTCCTAGAAG CAGAAAAGCT 
AACTCCTTCC GCACAACTTC AACACAGAAA ACAAAACAAA GGCGCTGGGG AAACTTCACC 
CAAGAGCCTC CGCAGCTCCA GAGCAACAAG AAATGCAAGA TTTTGCTGGA AGAACTTGCC 
AGTAGCGATC CCAAGTTAGC CCTCACTGGA GTCCCTATAG TACAGTGGCC AAAAAGGGAT 
AAGCTTAAAT TCCCTACCAG GCCAAAGGTG AGGGTTCCTA CAATTCCCAT CACAAAGCCT 
CACACCATGA AGCCAGCTCC ACGCTTAACA CCTGTAAGGC CTGCTGCAGC CTCCCCCATT 
GTGTCAGGAG CCAGGCGGAG AAGAGTGCGG TGCAGGAAAT GCAAAGCTTG TGTGCAAGGA 
GAATGTGGAG TCTGCCACTA CTGCAGGGAC ATGAAGAAAT TTGGTGGACC TGGACGCATG 
AAGCAATCCT GTGTCCTCCG ACAGTGCTTA GCACCCAGAC TGCCTCATTC AGTTACGTGT 
TCTCTCTGTG GAGAAGTAGA TCAGAATGAA GAGACCCAGG ACTTTGAAAA GAAACTCATG 
GAATGCTGCA TCTGCAACGA GATAGTTCAT CCTGGCTGCC TCCAGATGGA TGGAGAGGGG 
TTGCTGAACG AGGAATTGCC AAATTGCTGG GAGTGTCCAA AGTGTTACCA GGAAGACAGC 
TCGGACAAAG CCCAGAAGCG GAAAATAGAA GAGAGTGATG AAGAAGCTGT ACAAGCCAAA 
GTCTTACGGC CCCTGAGGAG CTGCGAGGAG CCTCTCACAC CCCCGCCTCA CTCACCTACT 
TCCATGCTGC AGCTCATCCA CGACCCGGTT TCTCCCCGGG GTATGGTGAC TCGGTCATCC 
CCTGGGGCTG GCCCCAGCGA CCACCACAGT GCCAGCCGTG ATGAACGCTT CAAACGGCGG 
CAGTTGCTGC GGCTACAAGC CACCGAGCGC ACCATGGTAC GGGAAAAGGA GAACAATCCC 
AGCGGCAAAA AGGAGCTGTC TGAAGTTGAG AAAGCCAAGA TCCGGGGATC GTACCTCACT 
GTCACTCTAC AGAGGCCCAC CAAAGAGCTC CACGGGACAT CCATTGTGCC CAAGCTGCAG 
GCCATCACGG CCTCCTCTGC CAACCTTCGC CCTAACCCCC GCGTGCTAAT GCAGCACTGC 
CCAGCCCGAA ACCCCCAGCA TGGGGATGAG GAGGGGCTTG GGGGAGAGGA GGAGGAAGAG 
GAGGAGGAGG AGGAAGATGA CAGTGCAGAG GAGGGGGGTG CAGCCAGGCT GAATGGCCGG 
GGCAGTTGGG CTCAGGATGG AGACGAAAGC TGGATGCAGC GGGAGGTCTG GATGTCTGTC 
TTCCGCTACC TCAGCCGCAA AGAACTTTGT GAATGTATGC GAGTGTGCAA GACATGGTAT 
AAATGGTGCT GTGATAAACG ACTTTGGACA AAAATTGACT TGAGTAGGTG TAAGGCCATC 
GTACCACAAG CTCTCAGTGG TATCATCAAG CGGCAGCCAG TAAGCCTCGA CCTCAGCTGG 
ACTAACATCT CCAAAAAGCA GCTGACATGG CTGGTCAATA GGCTGCCAGG ATTAAAAGAC 
CTCCTCCTAG CAGGCTGTTC CTGGTCTGCA GTATCTGCCC TCAGCACTTC CAGCTGCCCG 
CTTCTCAGGA CCCTTGATCT TCGGTGGGCA GTAGGAATTA AAGACCCTCA AATTCGGGAC 
TTGCTGACTC CACCCACAGA TAAGCCAGGT CAAGACAATC GAAGCAAACT CCGGAACATG 
ACTGACTTCC GGCTGGCAGG CCTTGACATC ACAGATGCTA CTCTCCGACT CATCATTCGC 
CACATGCCCC TTTTGTCTCG ACTTGACCTC AGTCACTGCA GTCACCTTAC AGATCAGTCC 
TCCAACCTAC TAACTGCTGT CGGGTCTTCC ACTCGATACT CCCTTACAGA GCTCAATATG 
GCAGGTTGCA ATAAATTGAC AGACCAGACC CTGTTCTTCC TAAGGCGAAT TGCTAATGTC 
ACCTTGATTG ACCTTCGAGG ATGCAAACAG ATCACGAGAA AAGCCTGTGA GCACTTCATC 
TCAGACTTGT CCATCAACAG CCTCTACTGC CTGTCTGATG AGAAACTGAT ACAGAAGATT 
AGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kdm2a ( NM_001001984.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001001984.2 Mus musculus lysine (K)-specific demethylase 2A (Kdm2a), mRNA.

Price & Availability↑

CloneID	OMu10441
Clone ID Related Accession (Same CDS sequence)	XM_006531722.4 , NM_001001984.2 , XM_006531722.3 , XM_006531722.2
Accession Version	NM_001001984.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	lysine-specific demethylase 2A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC140073.4, CB246224.1, BC076576.1 and AK173084.1. On May 24, 2006 this sequence version replaced NM_001001984.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164138, SAMN01164142 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAACCTG AAGAAGAAAG GATTCGGTAC AGCCAGAGAT TGCGTGGTAC CATGCGTCGT 
CGCTATGAAG ATGATGGCAT TTCAGATGAT GAAATTGAAG GGAAAAGAAC TTTTGACTTG 
GAAGAGAAGC TCCAAACCAA CAAATATAAT GCCAATTTTG TTACTTTTAT GGAGGGAAAA 
GATTTTAATG TAGAGTATAT CCAGCGGGGT GGCTTGAGAG ACCCTCTCAT TTTCAAGAAT 
TCTGATGGAC TTGGAATAAA GATGCCGGAT CCAGACTTCA CAGTGAATGA TGTCAAAATG 
TGTGTGGGGA GTCGTCGGAT GGTGGATGTC ATGGATGTGA ACACACAGAA GGGGATTGAA 
ATGACCATGG CACAATGGAC ACGATACTAT GAGACTCCAG AGGAAGAGCG AGAAAAACTC 
TATAATGTTA TCAGCCTAGA GTTTAGCCAC ACCAGGCTTG AGAATATGGT GCAGCGGCCT 
TCCACGGTGG ATTTCATTGA CTGGGTAGAT AACATGTGGC CAAGGCACTT GAAAGAAAGT 
CAGACAGAAT CAACAAATGC CATCTTAGAG ATGCAGTACC CTAAAGTGCA AAAGTACTGT 
CTAATGAGTG TTCGAGGCTG CTATACTGAC TTCCATGTGG ATTTTGGAGG TACTTCTGTT 
TGGTATCACA TCCACCAAGG TGGAAAGGTC TTCTGGCTCA TCCCCCCTAC AGCCCACAAC 
CTGGAGCTGT ACGAGAATTG GCTGCTATCA GGGAAACAGG GAGACATCTT TCTGGGTGAC 
CGGGTGTCAG ATTGCCAACG AATTGAGCTC AAGCAGGGCT ATACCTTCGT TATTCCCTCA 
GGTTGGATTC ATGCTGTGTA TACTCCTACA GACACATTAG TGTTTGGAGG CAATTTTTTG 
CATAGCTTCA ACATCCCCAT GCAATTAAAG ATATACAGCA TTGAAGATCG AACACGGGTT 
CCAAATAAAT TCCGTTACCC ATTTTACTAT GAAATGTGTT GGTATGTGTT GGAGCGCTAT 
GTATACTGCA TAACCAACCG ATCCCACCTA ACTAAGGATT TTCAGAAAGA ATCCCTTAGC 
ATGGATATGG AGTTAAATGA GTTGGAGTCT GGAAATGGTG ATGAGGAAGG GGTGGACAGA 
GAAGCCCGAC GCATGAACAA TAAGCGATCT GTGCTTACCA GCCCTGTTGC TAATGGAGTG 
AACCTGGATT ACGATGGACT TGGCAAAGCC TGCCGAAGTC TTCCAAGTCT GAAGAAAACT 
TTGTCTGGAG ACTCATCCTC AGACTCTACC CGGGGATCCC ACAATGGCCA AGTTTGGGAT 
CCCCAATGTA GCCCTAAAAA GGATAGGCAA GTGCATCTCA CCCATTTTGA ACTTGAAGGT 
CTTCGATGTC TTGTAGATAA GTTAGAGTCA CTGCCACTGC ACAAGAAGTG TGTCCCCACA 
GGAATAGAAG ACGAAGATGC TCTGATTGCT GATGTAAAGA TTTTGCTGGA AGAACTTGCC 
AGTAGCGATC CCAAGTTAGC CCTCACTGGA GTCCCTATAG TACAGTGGCC AAAAAGGGAT 
AAGCTTAAAT TCCCTACCAG GCCAAAGGTG AGGGTTCCTA CAATTCCCAT CACAAAGCCT 
CACACCATGA AGCCAGCTCC ACGCTTAACA CCTGTAAGGC CTGCTGCAGC CTCCCCCATT 
GTGTCAGGAG CCAGGCGGAG AAGAGTGCGG TGCAGGAAAT GCAAAGCTTG TGTGCAAGGA 
GAATGTGGAG TCTGCCACTA CTGCAGGGAC ATGAAGAAAT TTGGTGGACC TGGACGCATG 
AAGCAATCCT GTGTCCTCCG ACAGTGCTTA GCACCCAGAC TGCCTCATTC AGTTACGTGT 
TCTCTCTGTG GAGAAGTAGA TCAGAATGAA GAGACCCAGG ACTTTGAAAA GAAACTCATG 
GAATGCTGCA TCTGCAACGA GATAGTTCAT CCTGGCTGCC TCCAGATGGA TGGAGAGGGG 
TTGCTGAACG AGGAATTGCC AAATTGCTGG GAGTGTCCAA AGTGTTACCA GGAAGACAGC 
TCGGACAAAG CCCAGAAGCG GAAAATAGAA GAGAGTGATG AAGAAGCTGT ACAAGCCAAA 
GTCTTACGGC CCCTGAGGAG CTGCGAGGAG CCTCTCACAC CCCCGCCTCA CTCACCTACT 
TCCATGCTGC AGCTCATCCA CGACCCGGTT TCTCCCCGGG GTATGGTGAC TCGGTCATCC 
CCTGGGGCTG GCCCCAGCGA CCACCACAGT GCCAGCCGTG ATGAACGCTT CAAACGGCGG 
CAGTTGCTGC GGCTACAAGC CACCGAGCGC ACCATGGTAC GGGAAAAGGA GAACAATCCC 
AGCGGCAAAA AGGAGCTGTC TGAAGTTGAG AAAGCCAAGA TCCGGGGATC GTACCTCACT 
GTCACTCTAC AGAGGCCCAC CAAAGAGCTC CACGGGACAT CCATTGTGCC CAAGCTGCAG 
GCCATCACGG CCTCCTCTGC CAACCTTCGC CCTAACCCCC GCGTGCTAAT GCAGCACTGC 
CCAGCCCGAA ACCCCCAGCA TGGGGATGAG GAGGGGCTTG GGGGAGAGGA GGAGGAAGAG 
GAGGAGGAGG AGGAAGATGA CAGTGCAGAG GAGGGGGGTG CAGCCAGGCT GAATGGCCGG 
GGCAGTTGGG CTCAGGATGG AGACGAAAGC TGGATGCAGC GGGAGGTCTG GATGTCTGTC 
TTCCGCTACC TCAGCCGCAA AGAACTTTGT GAATGTATGC GAGTGTGCAA GACATGGTAT 
AAATGGTGCT GTGATAAACG ACTTTGGACA AAAATTGACT TGAGTAGGTG TAAGGCCATC 
GTACCACAAG CTCTCAGTGG TATCATCAAG CGGCAGCCAG TAAGCCTCGA CCTCAGCTGG 
ACTAACATCT CCAAAAAGCA GCTGACATGG CTGGTCAATA GGCTGCCAGG ATTAAAAGAC 
CTCCTCCTAG CAGGCTGTTC CTGGTCTGCA GTATCTGCCC TCAGCACTTC CAGCTGCCCG 
CTTCTCAGGA CCCTTGATCT TCGGTGGGCA GTAGGAATTA AAGACCCTCA AATTCGGGAC 
TTGCTGACTC CACCCACAGA TAAGCCAGGT CAAGACAATC GAAGCAAACT CCGGAACATG 
ACTGACTTCC GGCTGGCAGG CCTTGACATC ACAGATGCTA CTCTCCGACT CATCATTCGC 
CACATGCCCC TTTTGTCTCG ACTTGACCTC AGTCACTGCA GTCACCTTAC AGATCAGTCC 
TCCAACCTAC TAACTGCTGT CGGGTCTTCC ACTCGATACT CCCTTACAGA GCTCAATATG 
GCAGGTTGCA ATAAATTGAC AGACCAGACC CTGTTCTTCC TAAGGCGAAT TGCTAATGTC 
ACCTTGATTG ACCTTCGAGG ATGCAAACAG ATCACGAGAA AAGCCTGTGA GCACTTCATC 
TCAGACTTGT CCATCAACAG CCTCTACTGC CTGTCTGATG AGAAACTGAT ACAGAAGATT 
AGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001001984.2
CDS	827..4312
Translation	MEPEEERIRYSQRLRGTMRRRYEDDGISDDEIEGKRTFDLEEKLQTNKYNANFVTFMEGKDFNVEYIQRGGLRDPLIFKNSDGLGIKMPDPDFTVNDVKMCVGSRRMVDVMDVNTQKGIEMTMAQWTRYYETPEEEREKLYNVISLEFSHTRLENMVQRPSTVDFIDWVDNMWPRHLKESQTESTNAILEMQYPKVQKYCLMSVRGCYTDFHVDFGGTSVWYHIHQGGKVFWLIPPTAHNLELYENWLLSGKQGDIFLGDRVSDCQRIELKQGYTFVIPSGWIHAVYTPTDTLVFGGNFLHSFNIPMQLKIYSIEDRTRVPNKFRYPFYYEMCWYVLERYVYCITNRSHLTKDFQKESLSMDMELNELESGNGDEEGVDREARRMNNKRSVLTSPVANGVNLDYDGLGKACRSLPSLKKTLSGDSSSDSTRGSHNGQVWDPQCSPKKDRQVHLTHFELEGLRCLVDKLESLPLHKKCVPTGIEDEDALIADVKILLEELASSDPKLALTGVPIVQWPKRDKLKFPTRPKVRVPTIPITKPHTMKPAPRLTPVRPAAASPIVSGARRRRVRCRKCKACVQGECGVCHYCRDMKKFGGPGRMKQSCVLRQCLAPRLPHSVTCSLCGEVDQNEETQDFEKKLMECCICNEIVHPGCLQMDGEGLLNEELPNCWECPKCYQEDSSDKAQKRKIEESDEEAVQAKVLRPLRSCEEPLTPPPHSPTSMLQLIHDPVSPRGMVTRSSPGAGPSDHHSASRDERFKRRQLLRLQATERTMVREKENNPSGKKELSEVEKAKIRGSYLTVTLQRPTKELHGTSIVPKLQAITASSANLRPNPRVLMQHCPARNPQHGDEEGLGGEEEEEEEEEEDDSAEEGGAARLNGRGSWAQDGDESWMQREVWMSVFRYLSRKELCECMRVCKTWYKWCCDKRLWTKIDLSRCKAIVPQALSGIIKRQPVSLDLSWTNISKKQLTWLVNRLPGLKDLLLAGCSWSAVSALSTSSCPLLRTLDLRWAVGIKDPQIRDLLTPPTDKPGQDNRSKLRNMTDFRLAGLDITDATLRLIIRHMPLLSRLDLSHCSHLTDQSSNLLTAVGSSTRYSLTELNMAGCNKLTDQTLFFLRRIANVTLIDLRGCKQITRKACEHFISDLSINSLYCLSDEKLIQKIS

Target ORF information:

RefSeq Version	NM_001001984.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus lysine (K)-specific demethylase 2A (Kdm2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001001984.2

ORF Insert Sequence:

ATGGAACCTG AAGAAGAAAG GATTCGGTAC AGCCAGAGAT TGCGTGGTAC CATGCGTCGT 
CGCTATGAAG ATGATGGCAT TTCAGATGAT GAAATTGAAG GGAAAAGAAC TTTTGACTTG 
GAAGAGAAGC TCCAAACCAA CAAATATAAT GCCAATTTTG TTACTTTTAT GGAGGGAAAA 
GATTTTAATG TAGAGTATAT CCAGCGGGGT GGCTTGAGAG ACCCTCTCAT TTTCAAGAAT 
TCTGATGGAC TTGGAATAAA GATGCCGGAT CCAGACTTCA CAGTGAATGA TGTCAAAATG 
TGTGTGGGGA GTCGTCGGAT GGTGGATGTC ATGGATGTGA ACACACAGAA GGGGATTGAA 
ATGACCATGG CACAATGGAC ACGATACTAT GAGACTCCAG AGGAAGAGCG AGAAAAACTC 
TATAATGTTA TCAGCCTAGA GTTTAGCCAC ACCAGGCTTG AGAATATGGT GCAGCGGCCT 
TCCACGGTGG ATTTCATTGA CTGGGTAGAT AACATGTGGC CAAGGCACTT GAAAGAAAGT 
CAGACAGAAT CAACAAATGC CATCTTAGAG ATGCAGTACC CTAAAGTGCA AAAGTACTGT 
CTAATGAGTG TTCGAGGCTG CTATACTGAC TTCCATGTGG ATTTTGGAGG TACTTCTGTT 
TGGTATCACA TCCACCAAGG TGGAAAGGTC TTCTGGCTCA TCCCCCCTAC AGCCCACAAC 
CTGGAGCTGT ACGAGAATTG GCTGCTATCA GGGAAACAGG GAGACATCTT TCTGGGTGAC 
CGGGTGTCAG ATTGCCAACG AATTGAGCTC AAGCAGGGCT ATACCTTCGT TATTCCCTCA 
GGTTGGATTC ATGCTGTGTA TACTCCTACA GACACATTAG TGTTTGGAGG CAATTTTTTG 
CATAGCTTCA ACATCCCCAT GCAATTAAAG ATATACAGCA TTGAAGATCG AACACGGGTT 
CCAAATAAAT TCCGTTACCC ATTTTACTAT GAAATGTGTT GGTATGTGTT GGAGCGCTAT 
GTATACTGCA TAACCAACCG ATCCCACCTA ACTAAGGATT TTCAGAAAGA ATCCCTTAGC 
ATGGATATGG AGTTAAATGA GTTGGAGTCT GGAAATGGTG ATGAGGAAGG GGTGGACAGA 
GAAGCCCGAC GCATGAACAA TAAGCGATCT GTGCTTACCA GCCCTGTTGC TAATGGAGTG 
AACCTGGATT ACGATGGACT TGGCAAAGCC TGCCGAAGTC TTCCAAGTCT GAAGAAAACT 
TTGTCTGGAG ACTCATCCTC AGACTCTACC CGGGGATCCC ACAATGGCCA AGTTTGGGAT 
CCCCAATGTA GCCCTAAAAA GGATAGGCAA GTGCATCTCA CCCATTTTGA ACTTGAAGGT 
CTTCGATGTC TTGTAGATAA GTTAGAGTCA CTGCCACTGC ACAAGAAGTG TGTCCCCACA 
GGAATAGAAG ACGAAGATGC TCTGATTGCT GATGTAAAGA TTTTGCTGGA AGAACTTGCC 
AGTAGCGATC CCAAGTTAGC CCTCACTGGA GTCCCTATAG TACAGTGGCC AAAAAGGGAT 
AAGCTTAAAT TCCCTACCAG GCCAAAGGTG AGGGTTCCTA CAATTCCCAT CACAAAGCCT 
CACACCATGA AGCCAGCTCC ACGCTTAACA CCTGTAAGGC CTGCTGCAGC CTCCCCCATT 
GTGTCAGGAG CCAGGCGGAG AAGAGTGCGG TGCAGGAAAT GCAAAGCTTG TGTGCAAGGA 
GAATGTGGAG TCTGCCACTA CTGCAGGGAC ATGAAGAAAT TTGGTGGACC TGGACGCATG 
AAGCAATCCT GTGTCCTCCG ACAGTGCTTA GCACCCAGAC TGCCTCATTC AGTTACGTGT 
TCTCTCTGTG GAGAAGTAGA TCAGAATGAA GAGACCCAGG ACTTTGAAAA GAAACTCATG 
GAATGCTGCA TCTGCAACGA GATAGTTCAT CCTGGCTGCC TCCAGATGGA TGGAGAGGGG 
TTGCTGAACG AGGAATTGCC AAATTGCTGG GAGTGTCCAA AGTGTTACCA GGAAGACAGC 
TCGGACAAAG CCCAGAAGCG GAAAATAGAA GAGAGTGATG AAGAAGCTGT ACAAGCCAAA 
GTCTTACGGC CCCTGAGGAG CTGCGAGGAG CCTCTCACAC CCCCGCCTCA CTCACCTACT 
TCCATGCTGC AGCTCATCCA CGACCCGGTT TCTCCCCGGG GTATGGTGAC TCGGTCATCC 
CCTGGGGCTG GCCCCAGCGA CCACCACAGT GCCAGCCGTG ATGAACGCTT CAAACGGCGG 
CAGTTGCTGC GGCTACAAGC CACCGAGCGC ACCATGGTAC GGGAAAAGGA GAACAATCCC 
AGCGGCAAAA AGGAGCTGTC TGAAGTTGAG AAAGCCAAGA TCCGGGGATC GTACCTCACT 
GTCACTCTAC AGAGGCCCAC CAAAGAGCTC CACGGGACAT CCATTGTGCC CAAGCTGCAG 
GCCATCACGG CCTCCTCTGC CAACCTTCGC CCTAACCCCC GCGTGCTAAT GCAGCACTGC 
CCAGCCCGAA ACCCCCAGCA TGGGGATGAG GAGGGGCTTG GGGGAGAGGA GGAGGAAGAG 
GAGGAGGAGG AGGAAGATGA CAGTGCAGAG GAGGGGGGTG CAGCCAGGCT GAATGGCCGG 
GGCAGTTGGG CTCAGGATGG AGACGAAAGC TGGATGCAGC GGGAGGTCTG GATGTCTGTC 
TTCCGCTACC TCAGCCGCAA AGAACTTTGT GAATGTATGC GAGTGTGCAA GACATGGTAT 
AAATGGTGCT GTGATAAACG ACTTTGGACA AAAATTGACT TGAGTAGGTG TAAGGCCATC 
GTACCACAAG CTCTCAGTGG TATCATCAAG CGGCAGCCAG TAAGCCTCGA CCTCAGCTGG 
ACTAACATCT CCAAAAAGCA GCTGACATGG CTGGTCAATA GGCTGCCAGG ATTAAAAGAC 
CTCCTCCTAG CAGGCTGTTC CTGGTCTGCA GTATCTGCCC TCAGCACTTC CAGCTGCCCG 
CTTCTCAGGA CCCTTGATCT TCGGTGGGCA GTAGGAATTA AAGACCCTCA AATTCGGGAC 
TTGCTGACTC CACCCACAGA TAAGCCAGGT CAAGACAATC GAAGCAAACT CCGGAACATG 
ACTGACTTCC GGCTGGCAGG CCTTGACATC ACAGATGCTA CTCTCCGACT CATCATTCGC 
CACATGCCCC TTTTGTCTCG ACTTGACCTC AGTCACTGCA GTCACCTTAC AGATCAGTCC 
TCCAACCTAC TAACTGCTGT CGGGTCTTCC ACTCGATACT CCCTTACAGA GCTCAATATG 
GCAGGTTGCA ATAAATTGAC AGACCAGACC CTGTTCTTCC TAAGGCGAAT TGCTAATGTC 
ACCTTGATTG ACCTTCGAGG ATGCAAACAG ATCACGAGAA AAGCCTGTGA GCACTTCATC 
TCAGACTTGT CCATCAACAG CCTCTACTGC CTGTCTGATG AGAAACTGAT ACAGAAGATT 
AGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kdm2a ( XM_006531722.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006531785.1 Mus musculus lysine (K)-specific demethylase 2A (Kdm2a), transcript variant X1, mRNA.

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CloneID	OMu10441
Clone ID Related Accession (Same CDS sequence)	XM_006531722.4 , NM_001001984.2 , XM_006531722.3 , XM_006531722.2
Accession Version	XM_006531722.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	lysine-specific demethylase 2A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006531722.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACCTG AAGAAGAAAG GATTCGGTAC AGCCAGAGAT TGCGTGGTAC CATGCGTCGT 
CGCTATGAAG ATGATGGCAT TTCAGATGAT GAAATTGAAG GGAAAAGAAC TTTTGACTTG 
GAAGAGAAGC TCCAAACCAA CAAATATAAT GCCAATTTTG TTACTTTTAT GGAGGGAAAA 
GATTTTAATG TAGAGTATAT CCAGCGGGGT GGCTTGAGAG ACCCTCTCAT TTTCAAGAAT 
TCTGATGGAC TTGGAATAAA GATGCCGGAT CCAGACTTCA CAGTGAATGA TGTCAAAATG 
TGTGTGGGGA GTCGTCGGAT GGTGGATGTC ATGGATGTGA ACACACAGAA GGGGATTGAA 
ATGACCATGG CACAATGGAC ACGATACTAT GAGACTCCAG AGGAAGAGCG AGAAAAACTC 
TATAATGTTA TCAGCCTAGA GTTTAGCCAC ACCAGGCTTG AGAATATGGT GCAGCGGCCT 
TCCACGGTGG ATTTCATTGA CTGGGTAGAT AACATGTGGC CAAGGCACTT GAAAGAAAGT 
CAGACAGAAT CAACAAATGC CATCTTAGAG ATGCAGTACC CTAAAGTGCA AAAGTACTGT 
CTAATGAGTG TTCGAGGCTG CTATACTGAC TTCCATGTGG ATTTTGGAGG TACTTCTGTT 
TGGTATCACA TCCACCAAGG TGGAAAGGTC TTCTGGCTCA TCCCCCCTAC AGCCCACAAC 
CTGGAGCTGT ACGAGAATTG GCTGCTATCA GGGAAACAGG GAGACATCTT TCTGGGTGAC 
CGGGTGTCAG ATTGCCAACG AATTGAGCTC AAGCAGGGCT ATACCTTCGT TATTCCCTCA 
GGTTGGATTC ATGCTGTGTA TACTCCTACA GACACATTAG TGTTTGGAGG CAATTTTTTG 
CATAGCTTCA ACATCCCCAT GCAATTAAAG ATATACAGCA TTGAAGATCG AACACGGGTT 
CCAAATAAAT TCCGTTACCC ATTTTACTAT GAAATGTGTT GGTATGTGTT GGAGCGCTAT 
GTATACTGCA TAACCAACCG ATCCCACCTA ACTAAGGATT TTCAGAAAGA ATCCCTTAGC 
ATGGATATGG AGTTAAATGA GTTGGAGTCT GGAAATGGTG ATGAGGAAGG GGTGGACAGA 
GAAGCCCGAC GCATGAACAA TAAGCGATCT GTGCTTACCA GCCCTGTTGC TAATGGAGTG 
AACCTGGATT ACGATGGACT TGGCAAAGCC TGCCGAAGTC TTCCAAGTCT GAAGAAAACT 
TTGTCTGGAG ACTCATCCTC AGACTCTACC CGGGGATCCC ACAATGGCCA AGTTTGGGAT 
CCCCAATGTA GCCCTAAAAA GGATAGGCAA GTGCATCTCA CCCATTTTGA ACTTGAAGGT 
CTTCGATGTC TTGTAGATAA GTTAGAGTCA CTGCCACTGC ACAAGAAGTG TGTCCCCACA 
GGAATAGAAG ACGAAGATGC TCTGATTGCT GATGTAAAGA TTTTGCTGGA AGAACTTGCC 
AGTAGCGATC CCAAGTTAGC CCTCACTGGA GTCCCTATAG TACAGTGGCC AAAAAGGGAT 
AAGCTTAAAT TCCCTACCAG GCCAAAGGTG AGGGTTCCTA CAATTCCCAT CACAAAGCCT 
CACACCATGA AGCCAGCTCC ACGCTTAACA CCTGTAAGGC CTGCTGCAGC CTCCCCCATT 
GTGTCAGGAG CCAGGCGGAG AAGAGTGCGG TGCAGGAAAT GCAAAGCTTG TGTGCAAGGA 
GAATGTGGAG TCTGCCACTA CTGCAGGGAC ATGAAGAAAT TTGGTGGACC TGGACGCATG 
AAGCAATCCT GTGTCCTCCG ACAGTGCTTA GCACCCAGAC TGCCTCATTC AGTTACGTGT 
TCTCTCTGTG GAGAAGTAGA TCAGAATGAA GAGACCCAGG ACTTTGAAAA GAAACTCATG 
GAATGCTGCA TCTGCAACGA GATAGTTCAT CCTGGCTGCC TCCAGATGGA TGGAGAGGGG 
TTGCTGAACG AGGAATTGCC AAATTGCTGG GAGTGTCCAA AGTGTTACCA GGAAGACAGC 
TCGGACAAAG CCCAGAAGCG GAAAATAGAA GAGAGTGATG AAGAAGCTGT ACAAGCCAAA 
GTCTTACGGC CCCTGAGGAG CTGCGAGGAG CCTCTCACAC CCCCGCCTCA CTCACCTACT 
TCCATGCTGC AGCTCATCCA CGACCCGGTT TCTCCCCGGG GTATGGTGAC TCGGTCATCC 
CCTGGGGCTG GCCCCAGCGA CCACCACAGT GCCAGCCGTG ATGAACGCTT CAAACGGCGG 
CAGTTGCTGC GGCTACAAGC CACCGAGCGC ACCATGGTAC GGGAAAAGGA GAACAATCCC 
AGCGGCAAAA AGGAGCTGTC TGAAGTTGAG AAAGCCAAGA TCCGGGGATC GTACCTCACT 
GTCACTCTAC AGAGGCCCAC CAAAGAGCTC CACGGGACAT CCATTGTGCC CAAGCTGCAG 
GCCATCACGG CCTCCTCTGC CAACCTTCGC CCTAACCCCC GCGTGCTAAT GCAGCACTGC 
CCAGCCCGAA ACCCCCAGCA TGGGGATGAG GAGGGGCTTG GGGGAGAGGA GGAGGAAGAG 
GAGGAGGAGG AGGAAGATGA CAGTGCAGAG GAGGGGGGTG CAGCCAGGCT GAATGGCCGG 
GGCAGTTGGG CTCAGGATGG AGACGAAAGC TGGATGCAGC GGGAGGTCTG GATGTCTGTC 
TTCCGCTACC TCAGCCGCAA AGAACTTTGT GAATGTATGC GAGTGTGCAA GACATGGTAT 
AAATGGTGCT GTGATAAACG ACTTTGGACA AAAATTGACT TGAGTAGGTG TAAGGCCATC 
GTACCACAAG CTCTCAGTGG TATCATCAAG CGGCAGCCAG TAAGCCTCGA CCTCAGCTGG 
ACTAACATCT CCAAAAAGCA GCTGACATGG CTGGTCAATA GGCTGCCAGG ATTAAAAGAC 
CTCCTCCTAG CAGGCTGTTC CTGGTCTGCA GTATCTGCCC TCAGCACTTC CAGCTGCCCG 
CTTCTCAGGA CCCTTGATCT TCGGTGGGCA GTAGGAATTA AAGACCCTCA AATTCGGGAC 
TTGCTGACTC CACCCACAGA TAAGCCAGGT CAAGACAATC GAAGCAAACT CCGGAACATG 
ACTGACTTCC GGCTGGCAGG CCTTGACATC ACAGATGCTA CTCTCCGACT CATCATTCGC 
CACATGCCCC TTTTGTCTCG ACTTGACCTC AGTCACTGCA GTCACCTTAC AGATCAGTCC 
TCCAACCTAC TAACTGCTGT CGGGTCTTCC ACTCGATACT CCCTTACAGA GCTCAATATG 
GCAGGTTGCA ATAAATTGAC AGACCAGACC CTGTTCTTCC TAAGGCGAAT TGCTAATGTC 
ACCTTGATTG ACCTTCGAGG ATGCAAACAG ATCACGAGAA AAGCCTGTGA GCACTTCATC 
TCAGACTTGT CCATCAACAG CCTCTACTGC CTGTCTGATG AGAAACTGAT ACAGAAGATT 
AGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006531785.1
CDS	848..4333
Translation	MEPEEERIRYSQRLRGTMRRRYEDDGISDDEIEGKRTFDLEEKLQTNKYNANFVTFMEGKDFNVEYIQRGGLRDPLIFKNSDGLGIKMPDPDFTVNDVKMCVGSRRMVDVMDVNTQKGIEMTMAQWTRYYETPEEEREKLYNVISLEFSHTRLENMVQRPSTVDFIDWVDNMWPRHLKESQTESTNAILEMQYPKVQKYCLMSVRGCYTDFHVDFGGTSVWYHIHQGGKVFWLIPPTAHNLELYENWLLSGKQGDIFLGDRVSDCQRIELKQGYTFVIPSGWIHAVYTPTDTLVFGGNFLHSFNIPMQLKIYSIEDRTRVPNKFRYPFYYEMCWYVLERYVYCITNRSHLTKDFQKESLSMDMELNELESGNGDEEGVDREARRMNNKRSVLTSPVANGVNLDYDGLGKACRSLPSLKKTLSGDSSSDSTRGSHNGQVWDPQCSPKKDRQVHLTHFELEGLRCLVDKLESLPLHKKCVPTGIEDEDALIADVKILLEELASSDPKLALTGVPIVQWPKRDKLKFPTRPKVRVPTIPITKPHTMKPAPRLTPVRPAAASPIVSGARRRRVRCRKCKACVQGECGVCHYCRDMKKFGGPGRMKQSCVLRQCLAPRLPHSVTCSLCGEVDQNEETQDFEKKLMECCICNEIVHPGCLQMDGEGLLNEELPNCWECPKCYQEDSSDKAQKRKIEESDEEAVQAKVLRPLRSCEEPLTPPPHSPTSMLQLIHDPVSPRGMVTRSSPGAGPSDHHSASRDERFKRRQLLRLQATERTMVREKENNPSGKKELSEVEKAKIRGSYLTVTLQRPTKELHGTSIVPKLQAITASSANLRPNPRVLMQHCPARNPQHGDEEGLGGEEEEEEEEEEDDSAEEGGAARLNGRGSWAQDGDESWMQREVWMSVFRYLSRKELCECMRVCKTWYKWCCDKRLWTKIDLSRCKAIVPQALSGIIKRQPVSLDLSWTNISKKQLTWLVNRLPGLKDLLLAGCSWSAVSALSTSSCPLLRTLDLRWAVGIKDPQIRDLLTPPTDKPGQDNRSKLRNMTDFRLAGLDITDATLRLIIRHMPLLSRLDLSHCSHLTDQSSNLLTAVGSSTRYSLTELNMAGCNKLTDQTLFFLRRIANVTLIDLRGCKQITRKACEHFISDLSINSLYCLSDEKLIQKIS

Target ORF information:

RefSeq Version	XM_006531722.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus lysine (K)-specific demethylase 2A (Kdm2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006531722.4

ORF Insert Sequence:

ATGGAACCTG AAGAAGAAAG GATTCGGTAC AGCCAGAGAT TGCGTGGTAC CATGCGTCGT 
CGCTATGAAG ATGATGGCAT TTCAGATGAT GAAATTGAAG GGAAAAGAAC TTTTGACTTG 
GAAGAGAAGC TCCAAACCAA CAAATATAAT GCCAATTTTG TTACTTTTAT GGAGGGAAAA 
GATTTTAATG TAGAGTATAT CCAGCGGGGT GGCTTGAGAG ACCCTCTCAT TTTCAAGAAT 
TCTGATGGAC TTGGAATAAA GATGCCGGAT CCAGACTTCA CAGTGAATGA TGTCAAAATG 
TGTGTGGGGA GTCGTCGGAT GGTGGATGTC ATGGATGTGA ACACACAGAA GGGGATTGAA 
ATGACCATGG CACAATGGAC ACGATACTAT GAGACTCCAG AGGAAGAGCG AGAAAAACTC 
TATAATGTTA TCAGCCTAGA GTTTAGCCAC ACCAGGCTTG AGAATATGGT GCAGCGGCCT 
TCCACGGTGG ATTTCATTGA CTGGGTAGAT AACATGTGGC CAAGGCACTT GAAAGAAAGT 
CAGACAGAAT CAACAAATGC CATCTTAGAG ATGCAGTACC CTAAAGTGCA AAAGTACTGT 
CTAATGAGTG TTCGAGGCTG CTATACTGAC TTCCATGTGG ATTTTGGAGG TACTTCTGTT 
TGGTATCACA TCCACCAAGG TGGAAAGGTC TTCTGGCTCA TCCCCCCTAC AGCCCACAAC 
CTGGAGCTGT ACGAGAATTG GCTGCTATCA GGGAAACAGG GAGACATCTT TCTGGGTGAC 
CGGGTGTCAG ATTGCCAACG AATTGAGCTC AAGCAGGGCT ATACCTTCGT TATTCCCTCA 
GGTTGGATTC ATGCTGTGTA TACTCCTACA GACACATTAG TGTTTGGAGG CAATTTTTTG 
CATAGCTTCA ACATCCCCAT GCAATTAAAG ATATACAGCA TTGAAGATCG AACACGGGTT 
CCAAATAAAT TCCGTTACCC ATTTTACTAT GAAATGTGTT GGTATGTGTT GGAGCGCTAT 
GTATACTGCA TAACCAACCG ATCCCACCTA ACTAAGGATT TTCAGAAAGA ATCCCTTAGC 
ATGGATATGG AGTTAAATGA GTTGGAGTCT GGAAATGGTG ATGAGGAAGG GGTGGACAGA 
GAAGCCCGAC GCATGAACAA TAAGCGATCT GTGCTTACCA GCCCTGTTGC TAATGGAGTG 
AACCTGGATT ACGATGGACT TGGCAAAGCC TGCCGAAGTC TTCCAAGTCT GAAGAAAACT 
TTGTCTGGAG ACTCATCCTC AGACTCTACC CGGGGATCCC ACAATGGCCA AGTTTGGGAT 
CCCCAATGTA GCCCTAAAAA GGATAGGCAA GTGCATCTCA CCCATTTTGA ACTTGAAGGT 
CTTCGATGTC TTGTAGATAA GTTAGAGTCA CTGCCACTGC ACAAGAAGTG TGTCCCCACA 
GGAATAGAAG ACGAAGATGC TCTGATTGCT GATGTAAAGA TTTTGCTGGA AGAACTTGCC 
AGTAGCGATC CCAAGTTAGC CCTCACTGGA GTCCCTATAG TACAGTGGCC AAAAAGGGAT 
AAGCTTAAAT TCCCTACCAG GCCAAAGGTG AGGGTTCCTA CAATTCCCAT CACAAAGCCT 
CACACCATGA AGCCAGCTCC ACGCTTAACA CCTGTAAGGC CTGCTGCAGC CTCCCCCATT 
GTGTCAGGAG CCAGGCGGAG AAGAGTGCGG TGCAGGAAAT GCAAAGCTTG TGTGCAAGGA 
GAATGTGGAG TCTGCCACTA CTGCAGGGAC ATGAAGAAAT TTGGTGGACC TGGACGCATG 
AAGCAATCCT GTGTCCTCCG ACAGTGCTTA GCACCCAGAC TGCCTCATTC AGTTACGTGT 
TCTCTCTGTG GAGAAGTAGA TCAGAATGAA GAGACCCAGG ACTTTGAAAA GAAACTCATG 
GAATGCTGCA TCTGCAACGA GATAGTTCAT CCTGGCTGCC TCCAGATGGA TGGAGAGGGG 
TTGCTGAACG AGGAATTGCC AAATTGCTGG GAGTGTCCAA AGTGTTACCA GGAAGACAGC 
TCGGACAAAG CCCAGAAGCG GAAAATAGAA GAGAGTGATG AAGAAGCTGT ACAAGCCAAA 
GTCTTACGGC CCCTGAGGAG CTGCGAGGAG CCTCTCACAC CCCCGCCTCA CTCACCTACT 
TCCATGCTGC AGCTCATCCA CGACCCGGTT TCTCCCCGGG GTATGGTGAC TCGGTCATCC 
CCTGGGGCTG GCCCCAGCGA CCACCACAGT GCCAGCCGTG ATGAACGCTT CAAACGGCGG 
CAGTTGCTGC GGCTACAAGC CACCGAGCGC ACCATGGTAC GGGAAAAGGA GAACAATCCC 
AGCGGCAAAA AGGAGCTGTC TGAAGTTGAG AAAGCCAAGA TCCGGGGATC GTACCTCACT 
GTCACTCTAC AGAGGCCCAC CAAAGAGCTC CACGGGACAT CCATTGTGCC CAAGCTGCAG 
GCCATCACGG CCTCCTCTGC CAACCTTCGC CCTAACCCCC GCGTGCTAAT GCAGCACTGC 
CCAGCCCGAA ACCCCCAGCA TGGGGATGAG GAGGGGCTTG GGGGAGAGGA GGAGGAAGAG 
GAGGAGGAGG AGGAAGATGA CAGTGCAGAG GAGGGGGGTG CAGCCAGGCT GAATGGCCGG 
GGCAGTTGGG CTCAGGATGG AGACGAAAGC TGGATGCAGC GGGAGGTCTG GATGTCTGTC 
TTCCGCTACC TCAGCCGCAA AGAACTTTGT GAATGTATGC GAGTGTGCAA GACATGGTAT 
AAATGGTGCT GTGATAAACG ACTTTGGACA AAAATTGACT TGAGTAGGTG TAAGGCCATC 
GTACCACAAG CTCTCAGTGG TATCATCAAG CGGCAGCCAG TAAGCCTCGA CCTCAGCTGG 
ACTAACATCT CCAAAAAGCA GCTGACATGG CTGGTCAATA GGCTGCCAGG ATTAAAAGAC 
CTCCTCCTAG CAGGCTGTTC CTGGTCTGCA GTATCTGCCC TCAGCACTTC CAGCTGCCCG 
CTTCTCAGGA CCCTTGATCT TCGGTGGGCA GTAGGAATTA AAGACCCTCA AATTCGGGAC 
TTGCTGACTC CACCCACAGA TAAGCCAGGT CAAGACAATC GAAGCAAACT CCGGAACATG 
ACTGACTTCC GGCTGGCAGG CCTTGACATC ACAGATGCTA CTCTCCGACT CATCATTCGC 
CACATGCCCC TTTTGTCTCG ACTTGACCTC AGTCACTGCA GTCACCTTAC AGATCAGTCC 
TCCAACCTAC TAACTGCTGT CGGGTCTTCC ACTCGATACT CCCTTACAGA GCTCAATATG 
GCAGGTTGCA ATAAATTGAC AGACCAGACC CTGTTCTTCC TAAGGCGAAT TGCTAATGTC 
ACCTTGATTG ACCTTCGAGG ATGCAAACAG ATCACGAGAA AAGCCTGTGA GCACTTCATC 
TCAGACTTGT CCATCAACAG CCTCTACTGC CTGTCTGATG AGAAACTGAT ACAGAAGATT 
AGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kdm2a ( XM_006531722.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006531785.1 Mus musculus lysine (K)-specific demethylase 2A (Kdm2a), transcript variant X1, mRNA.

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CloneID	OMu10441
Clone ID Related Accession (Same CDS sequence)	XM_006531722.4 , NM_001001984.2 , XM_006531722.3 , XM_006531722.2
Accession Version	XM_006531722.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	lysine-specific demethylase 2A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000085.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006531722.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACCTG AAGAAGAAAG GATTCGGTAC AGCCAGAGAT TGCGTGGTAC CATGCGTCGT 
CGCTATGAAG ATGATGGCAT TTCAGATGAT GAAATTGAAG GGAAAAGAAC TTTTGACTTG 
GAAGAGAAGC TCCAAACCAA CAAATATAAT GCCAATTTTG TTACTTTTAT GGAGGGAAAA 
GATTTTAATG TAGAGTATAT CCAGCGGGGT GGCTTGAGAG ACCCTCTCAT TTTCAAGAAT 
TCTGATGGAC TTGGAATAAA GATGCCGGAT CCAGACTTCA CAGTGAATGA TGTCAAAATG 
TGTGTGGGGA GTCGTCGGAT GGTGGATGTC ATGGATGTGA ACACACAGAA GGGGATTGAA 
ATGACCATGG CACAATGGAC ACGATACTAT GAGACTCCAG AGGAAGAGCG AGAAAAACTC 
TATAATGTTA TCAGCCTAGA GTTTAGCCAC ACCAGGCTTG AGAATATGGT GCAGCGGCCT 
TCCACGGTGG ATTTCATTGA CTGGGTAGAT AACATGTGGC CAAGGCACTT GAAAGAAAGT 
CAGACAGAAT CAACAAATGC CATCTTAGAG ATGCAGTACC CTAAAGTGCA AAAGTACTGT 
CTAATGAGTG TTCGAGGCTG CTATACTGAC TTCCATGTGG ATTTTGGAGG TACTTCTGTT 
TGGTATCACA TCCACCAAGG TGGAAAGGTC TTCTGGCTCA TCCCCCCTAC AGCCCACAAC 
CTGGAGCTGT ACGAGAATTG GCTGCTATCA GGGAAACAGG GAGACATCTT TCTGGGTGAC 
CGGGTGTCAG ATTGCCAACG AATTGAGCTC AAGCAGGGCT ATACCTTCGT TATTCCCTCA 
GGTTGGATTC ATGCTGTGTA TACTCCTACA GACACATTAG TGTTTGGAGG CAATTTTTTG 
CATAGCTTCA ACATCCCCAT GCAATTAAAG ATATACAGCA TTGAAGATCG AACACGGGTT 
CCAAATAAAT TCCGTTACCC ATTTTACTAT GAAATGTGTT GGTATGTGTT GGAGCGCTAT 
GTATACTGCA TAACCAACCG ATCCCACCTA ACTAAGGATT TTCAGAAAGA ATCCCTTAGC 
ATGGATATGG AGTTAAATGA GTTGGAGTCT GGAAATGGTG ATGAGGAAGG GGTGGACAGA 
GAAGCCCGAC GCATGAACAA TAAGCGATCT GTGCTTACCA GCCCTGTTGC TAATGGAGTG 
AACCTGGATT ACGATGGACT TGGCAAAGCC TGCCGAAGTC TTCCAAGTCT GAAGAAAACT 
TTGTCTGGAG ACTCATCCTC AGACTCTACC CGGGGATCCC ACAATGGCCA AGTTTGGGAT 
CCCCAATGTA GCCCTAAAAA GGATAGGCAA GTGCATCTCA CCCATTTTGA ACTTGAAGGT 
CTTCGATGTC TTGTAGATAA GTTAGAGTCA CTGCCACTGC ACAAGAAGTG TGTCCCCACA 
GGAATAGAAG ACGAAGATGC TCTGATTGCT GATGTAAAGA TTTTGCTGGA AGAACTTGCC 
AGTAGCGATC CCAAGTTAGC CCTCACTGGA GTCCCTATAG TACAGTGGCC AAAAAGGGAT 
AAGCTTAAAT TCCCTACCAG GCCAAAGGTG AGGGTTCCTA CAATTCCCAT CACAAAGCCT 
CACACCATGA AGCCAGCTCC ACGCTTAACA CCTGTAAGGC CTGCTGCAGC CTCCCCCATT 
GTGTCAGGAG CCAGGCGGAG AAGAGTGCGG TGCAGGAAAT GCAAAGCTTG TGTGCAAGGA 
GAATGTGGAG TCTGCCACTA CTGCAGGGAC ATGAAGAAAT TTGGTGGACC TGGACGCATG 
AAGCAATCCT GTGTCCTCCG ACAGTGCTTA GCACCCAGAC TGCCTCATTC AGTTACGTGT 
TCTCTCTGTG GAGAAGTAGA TCAGAATGAA GAGACCCAGG ACTTTGAAAA GAAACTCATG 
GAATGCTGCA TCTGCAACGA GATAGTTCAT CCTGGCTGCC TCCAGATGGA TGGAGAGGGG 
TTGCTGAACG AGGAATTGCC AAATTGCTGG GAGTGTCCAA AGTGTTACCA GGAAGACAGC 
TCGGACAAAG CCCAGAAGCG GAAAATAGAA GAGAGTGATG AAGAAGCTGT ACAAGCCAAA 
GTCTTACGGC CCCTGAGGAG CTGCGAGGAG CCTCTCACAC CCCCGCCTCA CTCACCTACT 
TCCATGCTGC AGCTCATCCA CGACCCGGTT TCTCCCCGGG GTATGGTGAC TCGGTCATCC 
CCTGGGGCTG GCCCCAGCGA CCACCACAGT GCCAGCCGTG ATGAACGCTT CAAACGGCGG 
CAGTTGCTGC GGCTACAAGC CACCGAGCGC ACCATGGTAC GGGAAAAGGA GAACAATCCC 
AGCGGCAAAA AGGAGCTGTC TGAAGTTGAG AAAGCCAAGA TCCGGGGATC GTACCTCACT 
GTCACTCTAC AGAGGCCCAC CAAAGAGCTC CACGGGACAT CCATTGTGCC CAAGCTGCAG 
GCCATCACGG CCTCCTCTGC CAACCTTCGC CCTAACCCCC GCGTGCTAAT GCAGCACTGC 
CCAGCCCGAA ACCCCCAGCA TGGGGATGAG GAGGGGCTTG GGGGAGAGGA GGAGGAAGAG 
GAGGAGGAGG AGGAAGATGA CAGTGCAGAG GAGGGGGGTG CAGCCAGGCT GAATGGCCGG 
GGCAGTTGGG CTCAGGATGG AGACGAAAGC TGGATGCAGC GGGAGGTCTG GATGTCTGTC 
TTCCGCTACC TCAGCCGCAA AGAACTTTGT GAATGTATGC GAGTGTGCAA GACATGGTAT 
AAATGGTGCT GTGATAAACG ACTTTGGACA AAAATTGACT TGAGTAGGTG TAAGGCCATC 
GTACCACAAG CTCTCAGTGG TATCATCAAG CGGCAGCCAG TAAGCCTCGA CCTCAGCTGG 
ACTAACATCT CCAAAAAGCA GCTGACATGG CTGGTCAATA GGCTGCCAGG ATTAAAAGAC 
CTCCTCCTAG CAGGCTGTTC CTGGTCTGCA GTATCTGCCC TCAGCACTTC CAGCTGCCCG 
CTTCTCAGGA CCCTTGATCT TCGGTGGGCA GTAGGAATTA AAGACCCTCA AATTCGGGAC 
TTGCTGACTC CACCCACAGA TAAGCCAGGT CAAGACAATC GAAGCAAACT CCGGAACATG 
ACTGACTTCC GGCTGGCAGG CCTTGACATC ACAGATGCTA CTCTCCGACT CATCATTCGC 
CACATGCCCC TTTTGTCTCG ACTTGACCTC AGTCACTGCA GTCACCTTAC AGATCAGTCC 
TCCAACCTAC TAACTGCTGT CGGGTCTTCC ACTCGATACT CCCTTACAGA GCTCAATATG 
GCAGGTTGCA ATAAATTGAC AGACCAGACC CTGTTCTTCC TAAGGCGAAT TGCTAATGTC 
ACCTTGATTG ACCTTCGAGG ATGCAAACAG ATCACGAGAA AAGCCTGTGA GCACTTCATC 
TCAGACTTGT CCATCAACAG CCTCTACTGC CTGTCTGATG AGAAACTGAT ACAGAAGATT 
AGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006531785.1
CDS	723..4208
Translation	MEPEEERIRYSQRLRGTMRRRYEDDGISDDEIEGKRTFDLEEKLQTNKYNANFVTFMEGKDFNVEYIQRGGLRDPLIFKNSDGLGIKMPDPDFTVNDVKMCVGSRRMVDVMDVNTQKGIEMTMAQWTRYYETPEEEREKLYNVISLEFSHTRLENMVQRPSTVDFIDWVDNMWPRHLKESQTESTNAILEMQYPKVQKYCLMSVRGCYTDFHVDFGGTSVWYHIHQGGKVFWLIPPTAHNLELYENWLLSGKQGDIFLGDRVSDCQRIELKQGYTFVIPSGWIHAVYTPTDTLVFGGNFLHSFNIPMQLKIYSIEDRTRVPNKFRYPFYYEMCWYVLERYVYCITNRSHLTKDFQKESLSMDMELNELESGNGDEEGVDREARRMNNKRSVLTSPVANGVNLDYDGLGKACRSLPSLKKTLSGDSSSDSTRGSHNGQVWDPQCSPKKDRQVHLTHFELEGLRCLVDKLESLPLHKKCVPTGIEDEDALIADVKILLEELASSDPKLALTGVPIVQWPKRDKLKFPTRPKVRVPTIPITKPHTMKPAPRLTPVRPAAASPIVSGARRRRVRCRKCKACVQGECGVCHYCRDMKKFGGPGRMKQSCVLRQCLAPRLPHSVTCSLCGEVDQNEETQDFEKKLMECCICNEIVHPGCLQMDGEGLLNEELPNCWECPKCYQEDSSDKAQKRKIEESDEEAVQAKVLRPLRSCEEPLTPPPHSPTSMLQLIHDPVSPRGMVTRSSPGAGPSDHHSASRDERFKRRQLLRLQATERTMVREKENNPSGKKELSEVEKAKIRGSYLTVTLQRPTKELHGTSIVPKLQAITASSANLRPNPRVLMQHCPARNPQHGDEEGLGGEEEEEEEEEEDDSAEEGGAARLNGRGSWAQDGDESWMQREVWMSVFRYLSRKELCECMRVCKTWYKWCCDKRLWTKIDLSRCKAIVPQALSGIIKRQPVSLDLSWTNISKKQLTWLVNRLPGLKDLLLAGCSWSAVSALSTSSCPLLRTLDLRWAVGIKDPQIRDLLTPPTDKPGQDNRSKLRNMTDFRLAGLDITDATLRLIIRHMPLLSRLDLSHCSHLTDQSSNLLTAVGSSTRYSLTELNMAGCNKLTDQTLFFLRRIANVTLIDLRGCKQITRKACEHFISDLSINSLYCLSDEKLIQKIS

Target ORF information:

RefSeq Version	XM_006531722.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus lysine (K)-specific demethylase 2A (Kdm2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006531722.3

ORF Insert Sequence:

ATGGAACCTG AAGAAGAAAG GATTCGGTAC AGCCAGAGAT TGCGTGGTAC CATGCGTCGT 
CGCTATGAAG ATGATGGCAT TTCAGATGAT GAAATTGAAG GGAAAAGAAC TTTTGACTTG 
GAAGAGAAGC TCCAAACCAA CAAATATAAT GCCAATTTTG TTACTTTTAT GGAGGGAAAA 
GATTTTAATG TAGAGTATAT CCAGCGGGGT GGCTTGAGAG ACCCTCTCAT TTTCAAGAAT 
TCTGATGGAC TTGGAATAAA GATGCCGGAT CCAGACTTCA CAGTGAATGA TGTCAAAATG 
TGTGTGGGGA GTCGTCGGAT GGTGGATGTC ATGGATGTGA ACACACAGAA GGGGATTGAA 
ATGACCATGG CACAATGGAC ACGATACTAT GAGACTCCAG AGGAAGAGCG AGAAAAACTC 
TATAATGTTA TCAGCCTAGA GTTTAGCCAC ACCAGGCTTG AGAATATGGT GCAGCGGCCT 
TCCACGGTGG ATTTCATTGA CTGGGTAGAT AACATGTGGC CAAGGCACTT GAAAGAAAGT 
CAGACAGAAT CAACAAATGC CATCTTAGAG ATGCAGTACC CTAAAGTGCA AAAGTACTGT 
CTAATGAGTG TTCGAGGCTG CTATACTGAC TTCCATGTGG ATTTTGGAGG TACTTCTGTT 
TGGTATCACA TCCACCAAGG TGGAAAGGTC TTCTGGCTCA TCCCCCCTAC AGCCCACAAC 
CTGGAGCTGT ACGAGAATTG GCTGCTATCA GGGAAACAGG GAGACATCTT TCTGGGTGAC 
CGGGTGTCAG ATTGCCAACG AATTGAGCTC AAGCAGGGCT ATACCTTCGT TATTCCCTCA 
GGTTGGATTC ATGCTGTGTA TACTCCTACA GACACATTAG TGTTTGGAGG CAATTTTTTG 
CATAGCTTCA ACATCCCCAT GCAATTAAAG ATATACAGCA TTGAAGATCG AACACGGGTT 
CCAAATAAAT TCCGTTACCC ATTTTACTAT GAAATGTGTT GGTATGTGTT GGAGCGCTAT 
GTATACTGCA TAACCAACCG ATCCCACCTA ACTAAGGATT TTCAGAAAGA ATCCCTTAGC 
ATGGATATGG AGTTAAATGA GTTGGAGTCT GGAAATGGTG ATGAGGAAGG GGTGGACAGA 
GAAGCCCGAC GCATGAACAA TAAGCGATCT GTGCTTACCA GCCCTGTTGC TAATGGAGTG 
AACCTGGATT ACGATGGACT TGGCAAAGCC TGCCGAAGTC TTCCAAGTCT GAAGAAAACT 
TTGTCTGGAG ACTCATCCTC AGACTCTACC CGGGGATCCC ACAATGGCCA AGTTTGGGAT 
CCCCAATGTA GCCCTAAAAA GGATAGGCAA GTGCATCTCA CCCATTTTGA ACTTGAAGGT 
CTTCGATGTC TTGTAGATAA GTTAGAGTCA CTGCCACTGC ACAAGAAGTG TGTCCCCACA 
GGAATAGAAG ACGAAGATGC TCTGATTGCT GATGTAAAGA TTTTGCTGGA AGAACTTGCC 
AGTAGCGATC CCAAGTTAGC CCTCACTGGA GTCCCTATAG TACAGTGGCC AAAAAGGGAT 
AAGCTTAAAT TCCCTACCAG GCCAAAGGTG AGGGTTCCTA CAATTCCCAT CACAAAGCCT 
CACACCATGA AGCCAGCTCC ACGCTTAACA CCTGTAAGGC CTGCTGCAGC CTCCCCCATT 
GTGTCAGGAG CCAGGCGGAG AAGAGTGCGG TGCAGGAAAT GCAAAGCTTG TGTGCAAGGA 
GAATGTGGAG TCTGCCACTA CTGCAGGGAC ATGAAGAAAT TTGGTGGACC TGGACGCATG 
AAGCAATCCT GTGTCCTCCG ACAGTGCTTA GCACCCAGAC TGCCTCATTC AGTTACGTGT 
TCTCTCTGTG GAGAAGTAGA TCAGAATGAA GAGACCCAGG ACTTTGAAAA GAAACTCATG 
GAATGCTGCA TCTGCAACGA GATAGTTCAT CCTGGCTGCC TCCAGATGGA TGGAGAGGGG 
TTGCTGAACG AGGAATTGCC AAATTGCTGG GAGTGTCCAA AGTGTTACCA GGAAGACAGC 
TCGGACAAAG CCCAGAAGCG GAAAATAGAA GAGAGTGATG AAGAAGCTGT ACAAGCCAAA 
GTCTTACGGC CCCTGAGGAG CTGCGAGGAG CCTCTCACAC CCCCGCCTCA CTCACCTACT 
TCCATGCTGC AGCTCATCCA CGACCCGGTT TCTCCCCGGG GTATGGTGAC TCGGTCATCC 
CCTGGGGCTG GCCCCAGCGA CCACCACAGT GCCAGCCGTG ATGAACGCTT CAAACGGCGG 
CAGTTGCTGC GGCTACAAGC CACCGAGCGC ACCATGGTAC GGGAAAAGGA GAACAATCCC 
AGCGGCAAAA AGGAGCTGTC TGAAGTTGAG AAAGCCAAGA TCCGGGGATC GTACCTCACT 
GTCACTCTAC AGAGGCCCAC CAAAGAGCTC CACGGGACAT CCATTGTGCC CAAGCTGCAG 
GCCATCACGG CCTCCTCTGC CAACCTTCGC CCTAACCCCC GCGTGCTAAT GCAGCACTGC 
CCAGCCCGAA ACCCCCAGCA TGGGGATGAG GAGGGGCTTG GGGGAGAGGA GGAGGAAGAG 
GAGGAGGAGG AGGAAGATGA CAGTGCAGAG GAGGGGGGTG CAGCCAGGCT GAATGGCCGG 
GGCAGTTGGG CTCAGGATGG AGACGAAAGC TGGATGCAGC GGGAGGTCTG GATGTCTGTC 
TTCCGCTACC TCAGCCGCAA AGAACTTTGT GAATGTATGC GAGTGTGCAA GACATGGTAT 
AAATGGTGCT GTGATAAACG ACTTTGGACA AAAATTGACT TGAGTAGGTG TAAGGCCATC 
GTACCACAAG CTCTCAGTGG TATCATCAAG CGGCAGCCAG TAAGCCTCGA CCTCAGCTGG 
ACTAACATCT CCAAAAAGCA GCTGACATGG CTGGTCAATA GGCTGCCAGG ATTAAAAGAC 
CTCCTCCTAG CAGGCTGTTC CTGGTCTGCA GTATCTGCCC TCAGCACTTC CAGCTGCCCG 
CTTCTCAGGA CCCTTGATCT TCGGTGGGCA GTAGGAATTA AAGACCCTCA AATTCGGGAC 
TTGCTGACTC CACCCACAGA TAAGCCAGGT CAAGACAATC GAAGCAAACT CCGGAACATG 
ACTGACTTCC GGCTGGCAGG CCTTGACATC ACAGATGCTA CTCTCCGACT CATCATTCGC 
CACATGCCCC TTTTGTCTCG ACTTGACCTC AGTCACTGCA GTCACCTTAC AGATCAGTCC 
TCCAACCTAC TAACTGCTGT CGGGTCTTCC ACTCGATACT CCCTTACAGA GCTCAATATG 
GCAGGTTGCA ATAAATTGAC AGACCAGACC CTGTTCTTCC TAAGGCGAAT TGCTAATGTC 
ACCTTGATTG ACCTTCGAGG ATGCAAACAG ATCACGAGAA AAGCCTGTGA GCACTTCATC 
TCAGACTTGT CCATCAACAG CCTCTACTGC CTGTCTGATG AGAAACTGAT ACAGAAGATT 
AGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kdm2a ( XM_006531722.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006531785.1 Mus musculus lysine (K)-specific demethylase 2A (Kdm2a), transcript variant X1, mRNA.

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CloneID	OMu10441
Clone ID Related Accession (Same CDS sequence)	XM_006531722.4 , NM_001001984.2 , XM_006531722.3 , XM_006531722.2
Accession Version	XM_006531722.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	lysine-specific demethylase 2A isoform X1
Comment

ATGGAACCTG AAGAAGAAAG GATTCGGTAC AGCCAGAGAT TGCGTGGTAC CATGCGTCGT 
CGCTATGAAG ATGATGGCAT TTCAGATGAT GAAATTGAAG GGAAAAGAAC TTTTGACTTG 
GAAGAGAAGC TCCAAACCAA CAAATATAAT GCCAATTTTG TTACTTTTAT GGAGGGAAAA 
GATTTTAATG TAGAGTATAT CCAGCGGGGT GGCTTGAGAG ACCCTCTCAT TTTCAAGAAT 
TCTGATGGAC TTGGAATAAA GATGCCGGAT CCAGACTTCA CAGTGAATGA TGTCAAAATG 
TGTGTGGGGA GTCGTCGGAT GGTGGATGTC ATGGATGTGA ACACACAGAA GGGGATTGAA 
ATGACCATGG CACAATGGAC ACGATACTAT GAGACTCCAG AGGAAGAGCG AGAAAAACTC 
TATAATGTTA TCAGCCTAGA GTTTAGCCAC ACCAGGCTTG AGAATATGGT GCAGCGGCCT 
TCCACGGTGG ATTTCATTGA CTGGGTAGAT AACATGTGGC CAAGGCACTT GAAAGAAAGT 
CAGACAGAAT CAACAAATGC CATCTTAGAG ATGCAGTACC CTAAAGTGCA AAAGTACTGT 
CTAATGAGTG TTCGAGGCTG CTATACTGAC TTCCATGTGG ATTTTGGAGG TACTTCTGTT 
TGGTATCACA TCCACCAAGG TGGAAAGGTC TTCTGGCTCA TCCCCCCTAC AGCCCACAAC 
CTGGAGCTGT ACGAGAATTG GCTGCTATCA GGGAAACAGG GAGACATCTT TCTGGGTGAC 
CGGGTGTCAG ATTGCCAACG AATTGAGCTC AAGCAGGGCT ATACCTTCGT TATTCCCTCA 
GGTTGGATTC ATGCTGTGTA TACTCCTACA GACACATTAG TGTTTGGAGG CAATTTTTTG 
CATAGCTTCA ACATCCCCAT GCAATTAAAG ATATACAGCA TTGAAGATCG AACACGGGTT 
CCAAATAAAT TCCGTTACCC ATTTTACTAT GAAATGTGTT GGTATGTGTT GGAGCGCTAT 
GTATACTGCA TAACCAACCG ATCCCACCTA ACTAAGGATT TTCAGAAAGA ATCCCTTAGC 
ATGGATATGG AGTTAAATGA GTTGGAGTCT GGAAATGGTG ATGAGGAAGG GGTGGACAGA 
GAAGCCCGAC GCATGAACAA TAAGCGATCT GTGCTTACCA GCCCTGTTGC TAATGGAGTG 
AACCTGGATT ACGATGGACT TGGCAAAGCC TGCCGAAGTC TTCCAAGTCT GAAGAAAACT 
TTGTCTGGAG ACTCATCCTC AGACTCTACC CGGGGATCCC ACAATGGCCA AGTTTGGGAT 
CCCCAATGTA GCCCTAAAAA GGATAGGCAA GTGCATCTCA CCCATTTTGA ACTTGAAGGT 
CTTCGATGTC TTGTAGATAA GTTAGAGTCA CTGCCACTGC ACAAGAAGTG TGTCCCCACA 
GGAATAGAAG ACGAAGATGC TCTGATTGCT GATGTAAAGA TTTTGCTGGA AGAACTTGCC 
AGTAGCGATC CCAAGTTAGC CCTCACTGGA GTCCCTATAG TACAGTGGCC AAAAAGGGAT 
AAGCTTAAAT TCCCTACCAG GCCAAAGGTG AGGGTTCCTA CAATTCCCAT CACAAAGCCT 
CACACCATGA AGCCAGCTCC ACGCTTAACA CCTGTAAGGC CTGCTGCAGC CTCCCCCATT 
GTGTCAGGAG CCAGGCGGAG AAGAGTGCGG TGCAGGAAAT GCAAAGCTTG TGTGCAAGGA 
GAATGTGGAG TCTGCCACTA CTGCAGGGAC ATGAAGAAAT TTGGTGGACC TGGACGCATG 
AAGCAATCCT GTGTCCTCCG ACAGTGCTTA GCACCCAGAC TGCCTCATTC AGTTACGTGT 
TCTCTCTGTG GAGAAGTAGA TCAGAATGAA GAGACCCAGG ACTTTGAAAA GAAACTCATG 
GAATGCTGCA TCTGCAACGA GATAGTTCAT CCTGGCTGCC TCCAGATGGA TGGAGAGGGG 
TTGCTGAACG AGGAATTGCC AAATTGCTGG GAGTGTCCAA AGTGTTACCA GGAAGACAGC 
TCGGACAAAG CCCAGAAGCG GAAAATAGAA GAGAGTGATG AAGAAGCTGT ACAAGCCAAA 
GTCTTACGGC CCCTGAGGAG CTGCGAGGAG CCTCTCACAC CCCCGCCTCA CTCACCTACT 
TCCATGCTGC AGCTCATCCA CGACCCGGTT TCTCCCCGGG GTATGGTGAC TCGGTCATCC 
CCTGGGGCTG GCCCCAGCGA CCACCACAGT GCCAGCCGTG ATGAACGCTT CAAACGGCGG 
CAGTTGCTGC GGCTACAAGC CACCGAGCGC ACCATGGTAC GGGAAAAGGA GAACAATCCC 
AGCGGCAAAA AGGAGCTGTC TGAAGTTGAG AAAGCCAAGA TCCGGGGATC GTACCTCACT 
GTCACTCTAC AGAGGCCCAC CAAAGAGCTC CACGGGACAT CCATTGTGCC CAAGCTGCAG 
GCCATCACGG CCTCCTCTGC CAACCTTCGC CCTAACCCCC GCGTGCTAAT GCAGCACTGC 
CCAGCCCGAA ACCCCCAGCA TGGGGATGAG GAGGGGCTTG GGGGAGAGGA GGAGGAAGAG 
GAGGAGGAGG AGGAAGATGA CAGTGCAGAG GAGGGGGGTG CAGCCAGGCT GAATGGCCGG 
GGCAGTTGGG CTCAGGATGG AGACGAAAGC TGGATGCAGC GGGAGGTCTG GATGTCTGTC 
TTCCGCTACC TCAGCCGCAA AGAACTTTGT GAATGTATGC GAGTGTGCAA GACATGGTAT 
AAATGGTGCT GTGATAAACG ACTTTGGACA AAAATTGACT TGAGTAGGTG TAAGGCCATC 
GTACCACAAG CTCTCAGTGG TATCATCAAG CGGCAGCCAG TAAGCCTCGA CCTCAGCTGG 
ACTAACATCT CCAAAAAGCA GCTGACATGG CTGGTCAATA GGCTGCCAGG ATTAAAAGAC 
CTCCTCCTAG CAGGCTGTTC CTGGTCTGCA GTATCTGCCC TCAGCACTTC CAGCTGCCCG 
CTTCTCAGGA CCCTTGATCT TCGGTGGGCA GTAGGAATTA AAGACCCTCA AATTCGGGAC 
TTGCTGACTC CACCCACAGA TAAGCCAGGT CAAGACAATC GAAGCAAACT CCGGAACATG 
ACTGACTTCC GGCTGGCAGG CCTTGACATC ACAGATGCTA CTCTCCGACT CATCATTCGC 
CACATGCCCC TTTTGTCTCG ACTTGACCTC AGTCACTGCA GTCACCTTAC AGATCAGTCC 
TCCAACCTAC TAACTGCTGT CGGGTCTTCC ACTCGATACT CCCTTACAGA GCTCAATATG 
GCAGGTTGCA ATAAATTGAC AGACCAGACC CTGTTCTTCC TAAGGCGAAT TGCTAATGTC 
ACCTTGATTG ACCTTCGAGG ATGCAAACAG ATCACGAGAA AAGCCTGTGA GCACTTCATC 
TCAGACTTGT CCATCAACAG CCTCTACTGC CTGTCTGATG AGAAACTGAT ACAGAAGATT 
AGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_006531785.1

Translation

MEPEEERIRYSQRLRGTMRRRYEDDGISDDEIEGKRTFDLEEKLQTNKYNANFVTFMEGKDFNVEYIQRGGLRDPLIFKNSDGLGIKMPDPDFTVNDVKMCVGSRRMVDVMDVNTQKGIEMTMAQWTRYYETPEEEREKLYNVISLEFSHTRLENMVQRPSTVDFIDWVDNMWPRHLKESQTESTNAILEMQYPKVQKYCLMSVRGCYTDFHVDFGGTSVWYHIHQGGKVFWLIPPTAHNLELYENWLLSGKQGDIFLGDRVSDCQRIELKQGYTFVIPSGWIHAVYTPTDTLVFGGNFLHSFNIPMQLKIYSIEDRTRVPNKFRYPFYYEMCWYVLERYVYCITNRSHLTKDFQKESLSMDMELNELESGNGDEEGVDREARRMNNKRSVLTSPVANGVNLDYDGLGKACRSLPSLKKTLSGDSSSDSTRGSHNGQVWDPQCSPKKDRQVHLTHFELEGLRCLVDKLESLPLHKKCVPTGIEDEDALIADVKILLEELASSDPKLALTGVPIVQWPKRDKLKFPTRPKVRVPTIPITKPHTMKPAPRLTPVRPAAASPIVSGARRRRVRCRKCKACVQGECGVCHYCRDMKKFGGPGRMKQSCVLRQCLAPRLPHSVTCSLCGEVDQNEETQDFEKKLMECCICNEIVHPGCLQMDGEGLLNEELPNCWECPKCYQEDSSDKAQKRKIEESDEEAVQAKVLRPLRSCEEPLTPPPHSPTSMLQLIHDPVSPRGMVTRSSPGAGPSDHHSASRDERFKRRQLLRLQATERTMVREKENNPSGKKELSEVEKAKIRGSYLTVTLQRPTKELHGTSIVPKLQAITASSANLRPNPRVLMQHCPARNPQHGDEEGLGGEEEEEEEEEEDDSAEEGGAARLNGRGSWAQDGDESWMQREVWMSVFRYLSRKELCECMRVCKTWYKWCCDKRLWTKIDLSRCKAIVPQALSGIIKRQPVSLDLSWTNISKKQLTWLVNRLPGLKDLLLAGCSWSAVSALSTSSCPLLRTLDLRWAVGIKDPQIRDLLTPPTDKPGQDNRSKLRNMTDFRLAGLDITDATLRLIIRHMPLLSRLDLSHCSHLTDQSSNLLTAVGSSTRYSLTELNMAGCNKLTDQTLFFLRRIANVTLIDLRGCKQITRKACEHFISDLSINSLYCLSDEKLIQKIS

Target ORF information:

RefSeq Version	XM_006531722.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus lysine (K)-specific demethylase 2A (Kdm2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006531722.2

ORF Insert Sequence:

ATGGAACCTG AAGAAGAAAG GATTCGGTAC AGCCAGAGAT TGCGTGGTAC CATGCGTCGT 
CGCTATGAAG ATGATGGCAT TTCAGATGAT GAAATTGAAG GGAAAAGAAC TTTTGACTTG 
GAAGAGAAGC TCCAAACCAA CAAATATAAT GCCAATTTTG TTACTTTTAT GGAGGGAAAA 
GATTTTAATG TAGAGTATAT CCAGCGGGGT GGCTTGAGAG ACCCTCTCAT TTTCAAGAAT 
TCTGATGGAC TTGGAATAAA GATGCCGGAT CCAGACTTCA CAGTGAATGA TGTCAAAATG 
TGTGTGGGGA GTCGTCGGAT GGTGGATGTC ATGGATGTGA ACACACAGAA GGGGATTGAA 
ATGACCATGG CACAATGGAC ACGATACTAT GAGACTCCAG AGGAAGAGCG AGAAAAACTC 
TATAATGTTA TCAGCCTAGA GTTTAGCCAC ACCAGGCTTG AGAATATGGT GCAGCGGCCT 
TCCACGGTGG ATTTCATTGA CTGGGTAGAT AACATGTGGC CAAGGCACTT GAAAGAAAGT 
CAGACAGAAT CAACAAATGC CATCTTAGAG ATGCAGTACC CTAAAGTGCA AAAGTACTGT 
CTAATGAGTG TTCGAGGCTG CTATACTGAC TTCCATGTGG ATTTTGGAGG TACTTCTGTT 
TGGTATCACA TCCACCAAGG TGGAAAGGTC TTCTGGCTCA TCCCCCCTAC AGCCCACAAC 
CTGGAGCTGT ACGAGAATTG GCTGCTATCA GGGAAACAGG GAGACATCTT TCTGGGTGAC 
CGGGTGTCAG ATTGCCAACG AATTGAGCTC AAGCAGGGCT ATACCTTCGT TATTCCCTCA 
GGTTGGATTC ATGCTGTGTA TACTCCTACA GACACATTAG TGTTTGGAGG CAATTTTTTG 
CATAGCTTCA ACATCCCCAT GCAATTAAAG ATATACAGCA TTGAAGATCG AACACGGGTT 
CCAAATAAAT TCCGTTACCC ATTTTACTAT GAAATGTGTT GGTATGTGTT GGAGCGCTAT 
GTATACTGCA TAACCAACCG ATCCCACCTA ACTAAGGATT TTCAGAAAGA ATCCCTTAGC 
ATGGATATGG AGTTAAATGA GTTGGAGTCT GGAAATGGTG ATGAGGAAGG GGTGGACAGA 
GAAGCCCGAC GCATGAACAA TAAGCGATCT GTGCTTACCA GCCCTGTTGC TAATGGAGTG 
AACCTGGATT ACGATGGACT TGGCAAAGCC TGCCGAAGTC TTCCAAGTCT GAAGAAAACT 
TTGTCTGGAG ACTCATCCTC AGACTCTACC CGGGGATCCC ACAATGGCCA AGTTTGGGAT 
CCCCAATGTA GCCCTAAAAA GGATAGGCAA GTGCATCTCA CCCATTTTGA ACTTGAAGGT 
CTTCGATGTC TTGTAGATAA GTTAGAGTCA CTGCCACTGC ACAAGAAGTG TGTCCCCACA 
GGAATAGAAG ACGAAGATGC TCTGATTGCT GATGTAAAGA TTTTGCTGGA AGAACTTGCC 
AGTAGCGATC CCAAGTTAGC CCTCACTGGA GTCCCTATAG TACAGTGGCC AAAAAGGGAT 
AAGCTTAAAT TCCCTACCAG GCCAAAGGTG AGGGTTCCTA CAATTCCCAT CACAAAGCCT 
CACACCATGA AGCCAGCTCC ACGCTTAACA CCTGTAAGGC CTGCTGCAGC CTCCCCCATT 
GTGTCAGGAG CCAGGCGGAG AAGAGTGCGG TGCAGGAAAT GCAAAGCTTG TGTGCAAGGA 
GAATGTGGAG TCTGCCACTA CTGCAGGGAC ATGAAGAAAT TTGGTGGACC TGGACGCATG 
AAGCAATCCT GTGTCCTCCG ACAGTGCTTA GCACCCAGAC TGCCTCATTC AGTTACGTGT 
TCTCTCTGTG GAGAAGTAGA TCAGAATGAA GAGACCCAGG ACTTTGAAAA GAAACTCATG 
GAATGCTGCA TCTGCAACGA GATAGTTCAT CCTGGCTGCC TCCAGATGGA TGGAGAGGGG 
TTGCTGAACG AGGAATTGCC AAATTGCTGG GAGTGTCCAA AGTGTTACCA GGAAGACAGC 
TCGGACAAAG CCCAGAAGCG GAAAATAGAA GAGAGTGATG AAGAAGCTGT ACAAGCCAAA 
GTCTTACGGC CCCTGAGGAG CTGCGAGGAG CCTCTCACAC CCCCGCCTCA CTCACCTACT 
TCCATGCTGC AGCTCATCCA CGACCCGGTT TCTCCCCGGG GTATGGTGAC TCGGTCATCC 
CCTGGGGCTG GCCCCAGCGA CCACCACAGT GCCAGCCGTG ATGAACGCTT CAAACGGCGG 
CAGTTGCTGC GGCTACAAGC CACCGAGCGC ACCATGGTAC GGGAAAAGGA GAACAATCCC 
AGCGGCAAAA AGGAGCTGTC TGAAGTTGAG AAAGCCAAGA TCCGGGGATC GTACCTCACT 
GTCACTCTAC AGAGGCCCAC CAAAGAGCTC CACGGGACAT CCATTGTGCC CAAGCTGCAG 
GCCATCACGG CCTCCTCTGC CAACCTTCGC CCTAACCCCC GCGTGCTAAT GCAGCACTGC 
CCAGCCCGAA ACCCCCAGCA TGGGGATGAG GAGGGGCTTG GGGGAGAGGA GGAGGAAGAG 
GAGGAGGAGG AGGAAGATGA CAGTGCAGAG GAGGGGGGTG CAGCCAGGCT GAATGGCCGG 
GGCAGTTGGG CTCAGGATGG AGACGAAAGC TGGATGCAGC GGGAGGTCTG GATGTCTGTC 
TTCCGCTACC TCAGCCGCAA AGAACTTTGT GAATGTATGC GAGTGTGCAA GACATGGTAT 
AAATGGTGCT GTGATAAACG ACTTTGGACA AAAATTGACT TGAGTAGGTG TAAGGCCATC 
GTACCACAAG CTCTCAGTGG TATCATCAAG CGGCAGCCAG TAAGCCTCGA CCTCAGCTGG 
ACTAACATCT CCAAAAAGCA GCTGACATGG CTGGTCAATA GGCTGCCAGG ATTAAAAGAC 
CTCCTCCTAG CAGGCTGTTC CTGGTCTGCA GTATCTGCCC TCAGCACTTC CAGCTGCCCG 
CTTCTCAGGA CCCTTGATCT TCGGTGGGCA GTAGGAATTA AAGACCCTCA AATTCGGGAC 
TTGCTGACTC CACCCACAGA TAAGCCAGGT CAAGACAATC GAAGCAAACT CCGGAACATG 
ACTGACTTCC GGCTGGCAGG CCTTGACATC ACAGATGCTA CTCTCCGACT CATCATTCGC 
CACATGCCCC TTTTGTCTCG ACTTGACCTC AGTCACTGCA GTCACCTTAC AGATCAGTCC 
TCCAACCTAC TAACTGCTGT CGGGTCTTCC ACTCGATACT CCCTTACAGA GCTCAATATG 
GCAGGTTGCA ATAAATTGAC AGACCAGACC CTGTTCTTCC TAAGGCGAAT TGCTAATGTC 
ACCTTGATTG ACCTTCGAGG ATGCAAACAG ATCACGAGAA AAGCCTGTGA GCACTTCATC 
TCAGACTTGT CCATCAACAG CCTCTACTGC CTGTCTGATG AGAAACTGAT ACAGAAGATT 
AGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Broad histone H3K4me3 domains in mouse oocytes modulate maternal-to-zygotic transition.
Nature537(7621)548-552(2016 09)
Dahl JA,Jung I,Aanes H,Greggains GD,Manaf A,Lerdrup M,Li G,Kuan S,Li B,Lee AY,Preissl S,Jermstad I,Haugen MH,Suganthan R,Bj?r?s M,Hansen K,Dalen KT,Fedorcsak P,Ren B,Klungland A

Spatial and temporal expression of histone demethylase, Kdm2a, during murine molar development.
Biotechnic & histochemistry : official publication of the Biological Stain Commission91(2)137-44(2016)
Yi Q,Cao Y,Liu OS,Lu YQ,Wang JS,Wang SL,Yao R,Fan ZP

The histone demethylase Fbxl11/Kdm2a plays an essential role in embryonic development by repressing cell-cycle regulators.
Mechanisms of development13531-42(2015 Feb)
Kawakami E,Tokunaga A,Ozawa M,Sakamoto R,Yoshida N

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Kdm2a cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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