Grp cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Grp
Entrez Gene ID	225642
Full Name	gastrin releasing peptide
Synonyms	BLP
General protein information	Preferred Names gastrin releasing peptide Names gastrin-releasing peptide
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 18 Map Location: 18 E1\|18 38.98 cM
Summary	This gene encodes a neuropeptide hormone that affects various biological processes such as neuroendocrine regulation, gastrointestinal secretion, nociception, cell proliferation and inflammation. The encoded protein undergoes proteolytic processing to generate multiple mature peptides with biological activity. [provided by RefSeq, Aug 2015].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006525886.3	XP_006525949.1	gastrin-releasing peptide isoform X1
NM_175012.4	NP_778177.1	gastrin-releasing peptide preproprotein
XM_006525887.2	XP_006525950.1	gastrin-releasing peptide isoform X2
XM_006525887.3	XP_006525950.1	gastrin-releasing peptide isoform X2
XM_006525886.4	XP_006525949.1	gastrin-releasing peptide isoform X1

Pathways from BioSystems

Homologies

	LOC696983	XP_001086970.1
Pan troglodytes (chimpanzee)	GRP	XP_001142031.1
Homo sapiens (human)	GRP	NP_001012531.1
Rattus norvegicus (Norway rat)	Grp	NP_598254.2
Gallus gallus (chicken)	GRP	NP_001264829.1
Mus musculus (house mouse)	Grp	NP_778177.1

Gene Ontology
Bibliography

Related articles in PubMed

Distinct roles of NMB and GRP in itch transmission.
Wan L, Jin H, Liu XY, Jeffry J, Barry DM, Shen KF, Peng JH, Liu XT, Jin JH, Sun Y, Kim R, Meng QT, Mo P, Yin J, Tao A, Bardoni R, Chen ZF
Scientific reports7(1)15466(2017 Nov)

Gastrin-releasing peptide and its receptor increase arthritis fibroblast-like synoviocytes invasiveness through activating the PI3K/AKT pathway.
Clarimundo VS, Farinon M, Ped? RT, Teixeira VON, N?r C, Gulko PS, Xavier RM, de Oliveira PG
Peptides9557-61(2017 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

GrpmRNA and protein are upregulated in dorsal root ganglias, but not in the spinal cord, of mice with chronic itch.
Title: Critical evaluation of the expression of gastrin-releasing peptide in dorsal root ganglia and spinal cord.

Fos and phosphorylation of extracellular signal-regulated kinases (ERK) were used to look for evidence that interneurons expressing GRP were activated following intradermal injection of chloroquine.
Title: Spinal neurons that contain gastrin-releasing peptide seldom express Fos or phosphorylate extracellular signal-regulated kinases in response to intradermal chloroquine.

GRP is expressed by a distinct population of excitatory interneurons in laminae I-II that are likely to be involved in the itch pathway
Title: Expression of gastrin-releasing peptide by excitatory interneurons in the mouse superficial dorsal horn.

BNP-NPRA signaling is involved in both itch and pain and does not function upstream of the GRP-GRPR dedicated neuronal pathway.
Title: B-type natriuretic peptide is neither itch-specific nor functions upstream of the GRP-GRPR signaling pathway.

The majority of dorsal spinal cord Grp is synthesized locally in dorsal spinal cord neurons while Nmb is highly expressed in pain- and itch-sensing dorsal root ganglion neurons.
Title: The majority of dorsal spinal cord gastrin releasing peptide is synthesized locally whereas neuromedin B is highly expressed in pain- and itch-sensing somatosensory neurons.

The following Grp gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Grp cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu16544	NM_175012.4 Latest version!	Mus musculus gastrin releasing peptide (Grp), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu16544	XM_006525886.4 Latest version!	Mus musculus gastrin releasing peptide (Grp), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OMu54553	XM_006525887.3 Latest version!	Mus musculus gastrin releasing peptide (Grp), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OMu54553	XM_006525887.2	Mus musculus gastrin releasing peptide (Grp), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu16544	XM_006525886.3	Mus musculus gastrin releasing peptide (Grp), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Grp ( NM_175012.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_778177.1 Mus musculus gastrin releasing peptide (Grp), mRNA.

Price & Availability↑

CloneID	OMu16544
Clone ID Related Accession (Same CDS sequence)	XM_006525886.3 , NM_175012.4 , XM_006525886.4
Accession Version	NM_175012.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-26
Organism	Mus musculus(house mouse)
Product	gastrin-releasing peptide preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK139345.1, AK133508.1 and BE944748.1. On Aug 7, 2015 this sequence version replaced NM_175012.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK133508.1, BC024515.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849376, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGCGGCT CGGAGCTCTC GCTCTTGCTG TTGGCTCTGG TCCTCTGCCA GGCGCCCCGG 
GGGCCAGCTG CCCCGGTGTC GACAGGCGCA GGTGGAGGCA CGGTCCTGGC TAAGATGTAT 
CCGCGCGGCA GTCACTGGGC TGTGGGACAC TTAATGGGAA AAAAGAGCAC GGATGAATCC 
CCGTCCCTGT ATGCGGCTGA CAGAGACGGC CTGAAGGAGC AGCTGAGGGG GTACGTCCGC 
TGGGAAGAAG CTGCAAGGGA TTTGCTGGAC CTCCTAGAAG CTGCTGGGAA CCAAAGCCAC 
CAGCCACCTC AGCATCCGCC TCTCAGTCTC CAGCCTACTT GGGATCCCGA GGACGGCAGC 
TACTTTAACG ACGTTCAAAC CGCTAAGTTG GTAGACTCTC TGCTCCAGGT TCTCAAGGAA 
AAGGGAGGAA CTGCCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_778177.1
CDS	122..562
Translation	MRGSELSLLLLALVLCQAPRGPAAPVSTGAGGGTVLAKMYPRGSHWAVGHLMGKKSTDESPSLYAADRDGLKEQLRGYVRWEEAARDLLDLLEAAGNQSHQPPQHPPLSLQPTWDPEDGSYFNDVQTAKLVDSLLQVLKEKGGTAS

Target ORF information:

RefSeq Version	NM_175012.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus gastrin releasing peptide (Grp), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175012.4

ORF Insert Sequence:

ATGCGCGGCT CGGAGCTCTC GCTCTTGCTG TTGGCTCTGG TCCTCTGCCA GGCGCCCCGG 
GGGCCAGCTG CCCCGGTGTC GACAGGCGCA GGTGGAGGCA CGGTCCTGGC TAAGATGTAT 
CCGCGCGGCA GTCACTGGGC TGTGGGACAC TTAATGGGAA AAAAGAGCAC GGATGAATCC 
CCGTCCCTGT ATGCGGCTGA CAGAGACGGC CTGAAGGAGC AGCTGAGGGG GTACGTCCGC 
TGGGAAGAAG CTGCAAGGGA TTTGCTGGAC CTCCTAGAAG CTGCTGGGAA CCAAAGCCAC 
CAGCCACCTC AGCATCCGCC TCTCAGTCTC CAGCCTACTT GGGATCCCGA GGACGGCAGC 
TACTTTAACG ACGTTCAAAC CGCTAAGTTG GTAGACTCTC TGCTCCAGGT TCTCAAGGAA 
AAGGGAGGAA CTGCCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Grp ( XM_006525886.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525949.1 Mus musculus gastrin releasing peptide (Grp), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu16544
Clone ID Related Accession (Same CDS sequence)	XM_006525886.3 , NM_175012.4 , XM_006525886.4
Accession Version	XM_006525886.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	gastrin-releasing peptide isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006525886.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCGGCT CGGAGCTCTC GCTCTTGCTG TTGGCTCTGG TCCTCTGCCA GGCGCCCCGG 
GGGCCAGCTG CCCCGGTGTC GACAGGCGCA GGTGGAGGCA CGGTCCTGGC TAAGATGTAT 
CCGCGCGGCA GTCACTGGGC TGTGGGACAC TTAATGGGAA AAAAGAGCAC GGATGAATCC 
CCGTCCCTGT ATGCGGCTGA CAGAGACGGC CTGAAGGAGC AGCTGAGGGG GTACGTCCGC 
TGGGAAGAAG CTGCAAGGGA TTTGCTGGAC CTCCTAGAAG CTGCTGGGAA CCAAAGCCAC 
CAGCCACCTC AGCATCCGCC TCTCAGTCTC CAGCCTACTT GGGATCCCGA GGACGGCAGC 
TACTTTAACG ACGTTCAAAC CGCTAAGTTG GTAGACTCTC TGCTCCAGGT TCTCAAGGAA 
AAGGGAGGAA CTGCCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525949.1
CDS	277..717
Translation	MRGSELSLLLLALVLCQAPRGPAAPVSTGAGGGTVLAKMYPRGSHWAVGHLMGKKSTDESPSLYAADRDGLKEQLRGYVRWEEAARDLLDLLEAAGNQSHQPPQHPPLSLQPTWDPEDGSYFNDVQTAKLVDSLLQVLKEKGGTAS

Target ORF information:

RefSeq Version	XM_006525886.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus gastrin releasing peptide (Grp), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525886.3

ORF Insert Sequence:

ATGCGCGGCT CGGAGCTCTC GCTCTTGCTG TTGGCTCTGG TCCTCTGCCA GGCGCCCCGG 
GGGCCAGCTG CCCCGGTGTC GACAGGCGCA GGTGGAGGCA CGGTCCTGGC TAAGATGTAT 
CCGCGCGGCA GTCACTGGGC TGTGGGACAC TTAATGGGAA AAAAGAGCAC GGATGAATCC 
CCGTCCCTGT ATGCGGCTGA CAGAGACGGC CTGAAGGAGC AGCTGAGGGG GTACGTCCGC 
TGGGAAGAAG CTGCAAGGGA TTTGCTGGAC CTCCTAGAAG CTGCTGGGAA CCAAAGCCAC 
CAGCCACCTC AGCATCCGCC TCTCAGTCTC CAGCCTACTT GGGATCCCGA GGACGGCAGC 
TACTTTAACG ACGTTCAAAC CGCTAAGTTG GTAGACTCTC TGCTCCAGGT TCTCAAGGAA 
AAGGGAGGAA CTGCCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Grp ( XM_006525887.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525950.1 Mus musculus gastrin releasing peptide (Grp), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu54553
Clone ID Related Accession (Same CDS sequence)	XM_006525887.2 , XM_006525887.3
Accession Version	XM_006525887.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 600bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	gastrin-releasing peptide isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006525887.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCGGCT CGGAGCTCTC GCTCTTGCTG TTGGCTCTGG TCCTCTGCCA GGCGCCCCGG 
GGGCCAGCTG CCCCGGTGTC GACAGGCGCA GGTGGAGGCA CGGTCCTGGC TAAGATGTAT 
CCGCGCGGCA GTCACTGGGC TGTGGGACAC TTAATGGGAA AAAAGAGCAC GGATGAATCC 
CCGTCCCTGT ATGCGGCTGA CAGAGACGGC CTGAAGGAGC AGCTGAGGGG GTACGTCCGC 
TGGGAAGAAG CTGCAAGGGA TTTGCTGGAC CTCCTAGAAG CTGCTGGGAA CCAAAGCCAC 
CAGCCACCTC AGCATCCGCC TCTCAGTCTC CAGCCTACTT GGGATCCCGA GGACGGCAGC 
TACTTTAACG ACGTTCAAAC CGATGGAGAA GTTGGTAGAC TCTCTGCTCC AGGTTCTCAA 
GGAAAAGGGA GGAACTGCCA GCTGAAGGGA CAACGACAAG GGCGGCTTCC AAGGAGCAAA 
ACAAAACCCT TCAAAGACTG CCTTCTGCAA ACGTCAACTC TACTGGACCA TCAACAAGAT 
TTCCTTTGTG CAATTTACTT AACTATTCTC CTTATCTTTC AACCGCAACT AAACTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525950.1
CDS	498..1097
Translation	MRGSELSLLLLALVLCQAPRGPAAPVSTGAGGGTVLAKMYPRGSHWAVGHLMGKKSTDESPSLYAADRDGLKEQLRGYVRWEEAARDLLDLLEAAGNQSHQPPQHPPLSLQPTWDPEDGSYFNDVQTDGEVGRLSAPGSQGKGRNCQLKGQRQGRLPRSKTKPFKDCLLQTSTLLDHQQDFLCAIYLTILLIFQPQLNS

Target ORF information:

RefSeq Version	XM_006525887.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus gastrin releasing peptide (Grp), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525887.2

ORF Insert Sequence:

ATGCGCGGCT CGGAGCTCTC GCTCTTGCTG TTGGCTCTGG TCCTCTGCCA GGCGCCCCGG 
GGGCCAGCTG CCCCGGTGTC GACAGGCGCA GGTGGAGGCA CGGTCCTGGC TAAGATGTAT 
CCGCGCGGCA GTCACTGGGC TGTGGGACAC TTAATGGGAA AAAAGAGCAC GGATGAATCC 
CCGTCCCTGT ATGCGGCTGA CAGAGACGGC CTGAAGGAGC AGCTGAGGGG GTACGTCCGC 
TGGGAAGAAG CTGCAAGGGA TTTGCTGGAC CTCCTAGAAG CTGCTGGGAA CCAAAGCCAC 
CAGCCACCTC AGCATCCGCC TCTCAGTCTC CAGCCTACTT GGGATCCCGA GGACGGCAGC 
TACTTTAACG ACGTTCAAAC CGATGGAGAA GTTGGTAGAC TCTCTGCTCC AGGTTCTCAA 
GGAAAAGGGA GGAACTGCCA GCTGAAGGGA CAACGACAAG GGCGGCTTCC AAGGAGCAAA 
ACAAAACCCT TCAAAGACTG CCTTCTGCAA ACGTCAACTC TACTGGACCA TCAACAAGAT 
TTCCTTTGTG CAATTTACTT AACTATTCTC CTTATCTTTC AACCGCAACT AAACTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Grp ( XM_006525886.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525949.1 Mus musculus gastrin releasing peptide (Grp), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu16544
Clone ID Related Accession (Same CDS sequence)	XM_006525886.3 , NM_175012.4 , XM_006525886.4
Accession Version	XM_006525886.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	gastrin-releasing peptide isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006525886.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCGGCT CGGAGCTCTC GCTCTTGCTG TTGGCTCTGG TCCTCTGCCA GGCGCCCCGG 
GGGCCAGCTG CCCCGGTGTC GACAGGCGCA GGTGGAGGCA CGGTCCTGGC TAAGATGTAT 
CCGCGCGGCA GTCACTGGGC TGTGGGACAC TTAATGGGAA AAAAGAGCAC GGATGAATCC 
CCGTCCCTGT ATGCGGCTGA CAGAGACGGC CTGAAGGAGC AGCTGAGGGG GTACGTCCGC 
TGGGAAGAAG CTGCAAGGGA TTTGCTGGAC CTCCTAGAAG CTGCTGGGAA CCAAAGCCAC 
CAGCCACCTC AGCATCCGCC TCTCAGTCTC CAGCCTACTT GGGATCCCGA GGACGGCAGC 
TACTTTAACG ACGTTCAAAC CGCTAAGTTG GTAGACTCTC TGCTCCAGGT TCTCAAGGAA 
AAGGGAGGAA CTGCCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525949.1
CDS	272..712
Translation	MRGSELSLLLLALVLCQAPRGPAAPVSTGAGGGTVLAKMYPRGSHWAVGHLMGKKSTDESPSLYAADRDGLKEQLRGYVRWEEAARDLLDLLEAAGNQSHQPPQHPPLSLQPTWDPEDGSYFNDVQTAKLVDSLLQVLKEKGGTAS

Target ORF information:

RefSeq Version	XM_006525886.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus gastrin releasing peptide (Grp), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525886.4

ORF Insert Sequence:

ATGCGCGGCT CGGAGCTCTC GCTCTTGCTG TTGGCTCTGG TCCTCTGCCA GGCGCCCCGG 
GGGCCAGCTG CCCCGGTGTC GACAGGCGCA GGTGGAGGCA CGGTCCTGGC TAAGATGTAT 
CCGCGCGGCA GTCACTGGGC TGTGGGACAC TTAATGGGAA AAAAGAGCAC GGATGAATCC 
CCGTCCCTGT ATGCGGCTGA CAGAGACGGC CTGAAGGAGC AGCTGAGGGG GTACGTCCGC 
TGGGAAGAAG CTGCAAGGGA TTTGCTGGAC CTCCTAGAAG CTGCTGGGAA CCAAAGCCAC 
CAGCCACCTC AGCATCCGCC TCTCAGTCTC CAGCCTACTT GGGATCCCGA GGACGGCAGC 
TACTTTAACG ACGTTCAAAC CGCTAAGTTG GTAGACTCTC TGCTCCAGGT TCTCAAGGAA 
AAGGGAGGAA CTGCCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Grp ( XM_006525887.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525950.1 Mus musculus gastrin releasing peptide (Grp), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu54553
Clone ID Related Accession (Same CDS sequence)	XM_006525887.2 , XM_006525887.3
Accession Version	XM_006525887.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 600bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	gastrin-releasing peptide isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006525887.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCGGCT CGGAGCTCTC GCTCTTGCTG TTGGCTCTGG TCCTCTGCCA GGCGCCCCGG 
GGGCCAGCTG CCCCGGTGTC GACAGGCGCA GGTGGAGGCA CGGTCCTGGC TAAGATGTAT 
CCGCGCGGCA GTCACTGGGC TGTGGGACAC TTAATGGGAA AAAAGAGCAC GGATGAATCC 
CCGTCCCTGT ATGCGGCTGA CAGAGACGGC CTGAAGGAGC AGCTGAGGGG GTACGTCCGC 
TGGGAAGAAG CTGCAAGGGA TTTGCTGGAC CTCCTAGAAG CTGCTGGGAA CCAAAGCCAC 
CAGCCACCTC AGCATCCGCC TCTCAGTCTC CAGCCTACTT GGGATCCCGA GGACGGCAGC 
TACTTTAACG ACGTTCAAAC CGATGGAGAA GTTGGTAGAC TCTCTGCTCC AGGTTCTCAA 
GGAAAAGGGA GGAACTGCCA GCTGAAGGGA CAACGACAAG GGCGGCTTCC AAGGAGCAAA 
ACAAAACCCT TCAAAGACTG CCTTCTGCAA ACGTCAACTC TACTGGACCA TCAACAAGAT 
TTCCTTTGTG CAATTTACTT AACTATTCTC CTTATCTTTC AACCGCAACT AAACTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525950.1
CDS	495..1094
Translation	MRGSELSLLLLALVLCQAPRGPAAPVSTGAGGGTVLAKMYPRGSHWAVGHLMGKKSTDESPSLYAADRDGLKEQLRGYVRWEEAARDLLDLLEAAGNQSHQPPQHPPLSLQPTWDPEDGSYFNDVQTDGEVGRLSAPGSQGKGRNCQLKGQRQGRLPRSKTKPFKDCLLQTSTLLDHQQDFLCAIYLTILLIFQPQLNS

Target ORF information:

RefSeq Version	XM_006525887.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus gastrin releasing peptide (Grp), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525887.3

ORF Insert Sequence:

ATGCGCGGCT CGGAGCTCTC GCTCTTGCTG TTGGCTCTGG TCCTCTGCCA GGCGCCCCGG 
GGGCCAGCTG CCCCGGTGTC GACAGGCGCA GGTGGAGGCA CGGTCCTGGC TAAGATGTAT 
CCGCGCGGCA GTCACTGGGC TGTGGGACAC TTAATGGGAA AAAAGAGCAC GGATGAATCC 
CCGTCCCTGT ATGCGGCTGA CAGAGACGGC CTGAAGGAGC AGCTGAGGGG GTACGTCCGC 
TGGGAAGAAG CTGCAAGGGA TTTGCTGGAC CTCCTAGAAG CTGCTGGGAA CCAAAGCCAC 
CAGCCACCTC AGCATCCGCC TCTCAGTCTC CAGCCTACTT GGGATCCCGA GGACGGCAGC 
TACTTTAACG ACGTTCAAAC CGATGGAGAA GTTGGTAGAC TCTCTGCTCC AGGTTCTCAA 
GGAAAAGGGA GGAACTGCCA GCTGAAGGGA CAACGACAAG GGCGGCTTCC AAGGAGCAAA 
ACAAAACCCT TCAAAGACTG CCTTCTGCAA ACGTCAACTC TACTGGACCA TCAACAAGAT 
TTCCTTTGTG CAATTTACTT AACTATTCTC CTTATCTTTC AACCGCAACT AAACTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Distinct roles of NMB and GRP in itch transmission.
Scientific reports7(1)15466(2017 Nov)
Wan L,Jin H,Liu XY,Jeffry J,Barry DM,Shen KF,Peng JH,Liu XT,Jin JH,Sun Y,Kim R,Meng QT,Mo P,Yin J,Tao A,Bardoni R,Chen ZF

Gastrin-releasing peptide and its receptor increase arthritis fibroblast-like synoviocytes invasiveness through activating the PI3K/AKT pathway.
Peptides9557-61(2017 Sep)
Clarimundo VS,Farinon M,Ped? RT,Teixeira VON,N?r C,Gulko PS,Xavier RM,de Oliveira PG

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Grp cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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