Zfp521 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Zfp521
Entrez Gene ID	225207
Full Name	zinc finger protein 521
Synonyms	B930086A16Rik,Evi3,Znf521
General protein information	Preferred Names zinc finger protein 521 Names zinc finger protein 521 ecotropic viral integration site 3 protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 18 Map Location: 18 A1\|18 7.68 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_011246894.1	XP_011245196.1	zinc finger protein 521 isoform X1
NM_145492.4	NP_663467.1	zinc finger protein 521 isoform 1
NM_001360804.1	NP_001347733.1	zinc finger protein 521 isoform 2
XM_006525820.4	XP_006525883.1	zinc finger protein 521 isoform X1
XM_006525820.3	XP_006525883.1	zinc finger protein 521 isoform X2
XM_006525819.3	XP_006525882.1	zinc finger protein 521 isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	ZNF521	NP_001098889.1
Mus musculus (house mouse)	Zfp521	NP_663467.1
Pan troglodytes (chimpanzee)	ZNF521	XP_512068.2
Rattus norvegicus (Norway rat)	Zfp521	NP_001100873.1
Canis lupus familiaris (dog)	ZNF521	XP_547633.2
Gallus gallus (chicken)	ZNF521	XP_004939860.1
Xenopus tropicalis (tropical clawed frog)	znf521	XP_004915266.1
Homo sapiens (human)	ZNF521	NP_056276.1
	LOC709169	XP_002800935.1

Gene Ontology
Bibliography

Related articles in PubMed

Conserved expression of ultra-conserved noncoding RNA in mammalian nervous system.
Zhou J, Wang R, Zhang J, Zhu L, Liu W, Lu S, Chen P, Li H, Yin B, Yuan J, Qiang B, Shu P, Peng X
Biochimica et biophysica acta1860(12)1159-1168(2017 Dec)

ZFP521 regulates murine hematopoietic stem cell function and facilitates MLL-AF9 leukemogenesis in mouse and human cells.
Garrison BS, Rybak AP, Beerman I, Heesters B, Mercier FE, Scadden DT, Bryder D, Baron R, Rossi DJ
Blood130(5)619-624(2017 08)

Foxo1 is a downstream effector of Isl1 in direct pathway striatal projection neuron development within the embryonic mouse telencephalon.
Waclaw RR, Ehrman LA, Merchan-Sala P, Kohli V, Nardini D, Campbell K
Molecular and cellular neurosciences8044-51(2017 04)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

these results identify ZNF521/ZFP521 as a critical regulator of HSC function, which facilitates MLL-AF9-mediated leukemic disease in mice.
Title: ZFP521 regulates murine hematopoietic stem cell function and facilitates MLL-AF9 leukemogenesis in mouse and human cells.

Here we demonstrate that the transcription factors SPI1 (PU.1) and HOXC13 synergistically regulate Zfp521 expression, and identify the regions of the Zfp521 promoter required for this transcriptional activity. We also show that SPI1 and HOXC13 activate Zfp521 in a dose-dependent manner.
Title: Transcriptional regulation of the proto-oncogene Zfp521 by SPI1 (PU.1) and HOXC13.

links between dysregulated Zfp521 and B-cell survival
Title: Zfp521 promotes B-cell viability and cyclin D1 gene expression in a B cell culture system.

Data suggest a facilitated approach for establishing human neural stem cells (NSCs) through zinc finger protein 521 (Zfp521)-driven conversion of fibroblasts.
Title: Conversion of Human Fibroblasts to Stably Self-Renewing Neural Stem Cells with a Single Zinc-Finger Transcription Factor.

it is concluding that an enhanced expression of Fndc5 in neural progenitor cells is stimulated by Zfp521 overexpression in these cells
Title: Zinc finger protein 521 overexpression increased transcript levels of Fndc5 in mouse embryonic stem cells.

The following Zfp521 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Zfp521 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu54502	XM_006525820.4 Latest version!	Mus musculus zinc finger protein 521 (Zfp521), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu11680	NM_145492.4 Latest version!	Mus musculus zinc finger protein 521 (Zfp521), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$769.30 $1099.00
OMu73236	NM_001360804.1 Latest version!	Mus musculus zinc finger protein 521 (Zfp521), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu54502	XM_006525819.3 Latest version!	Mus musculus zinc finger protein 521 (Zfp521), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu73236	XM_011246894.1 Latest version!	Mus musculus zinc finger protein 521 (Zfp521), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
View Old Accession Versions >>
OMu54502	XM_006525820.3	Mus musculus zinc finger protein 521 (Zfp521), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Zfp521 ( XM_006525820.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525883.1 Mus musculus zinc finger protein 521 (Zfp521), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu54502
Clone ID Related Accession (Same CDS sequence)	XM_006525820.4 , XM_006525820.3 , XM_006525819.3
Accession Version	XM_006525820.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	zinc finger protein 521 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006525820.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGTTC ATGAGCGGAA CAAGGACGGC TCCCAGTCTG GCTCCAGGAT GGAGGACTGG 
AAGATGAAGG ACACTCAGAA GTGCAGCCAG TGCGAGGAAG GCTTTGACTT CCCGGAAGAC 
CTCCAGAAGC ACATTGCAGA ATGCCACCCC GAGTGCTCCC CTAATGAGGA CCGAGCAGCC 
CTCCAGTGCA TGTACTGCCA CGAGCTGTTT GTGGAGGAGA CGTCCCTCAT GAACCACATC 
GAGCAGGTAC ACGGGGGTGA GAAGAAGAAC TCTTGCAGCA TTTGCTCAGA GAGCTTCCTC 
ACGGTCGAGG AGCTGTATAG CCACATGGAC AGTCACCAGC AGCCAGAGTC CTGCAATCAC 
AGCAACAGCC CTTCCCTGGT CACTGTGGGT TACACCTCAG TGTCCAGCAC GACTCCAGAC 
TCGAACCTCT CAGTGGACAG CTCGACCATG GTAGAGGCTG CACCACCGAT TCCAAAGAGC 
CGCGGGAGGA AGCGAGCTGC TCAGCAGACC TCCGATATGA CTGGCCCCTC TAGTAAGCAG 
GCGAAAGTCA CCTACAGCTG TATTTACTGC AACAAGCAGT TATTTTCCAG TCTCGCGGTT 
CTGCAGATTC ACTTGAAAAC TATGCATTTA GATAAGCCTG AGCAGGCTCA TATCTGTCAG 
TATTGCTTGG AGGTCTTACC CTCACTCTAT AACCTAAATG AACATCTTAA ACAAGTGCAC 
GAAGCTCAGG ACCCCGGCCT GATTGTTTCG GCCATGCCTG CCATTGTTTA CCAGTGCAAC 
TTCTGTTCCG AAGTTGTCAA TGACCTCAAC ACCCTTCAGG AGCACATCCG ATGTTCTCAT 
GGGTTTGCCA ATCCCGCGGC CAAGGACAGC AATGCGTTCT TTTGTCCCCA TTGTTACATG 
GGGTTTCTCA CTGACTCTTC ACTTGAAGAG CATATAAGAC AGGTCCATTG TGACCTCAGT 
GGCTCCCGGT TTGGGTCTCC TGTGCTTGGG ACTCCAAAAG AACCGGTGGT TGAAGTCTAC 
TCCTGTTCCT ATTGTACAAA TTCGCCAATA TTCAACAGTG TTCTTAAACT GAATAAGCAT 
ATTAAAGAGA ATCATAAAAA CATTCCCTTG GCCCTGAATT ATATTCACAA TGGGAAGAAA 
TCCCGGGCCT TGAGCCCTTT GTCCCCTGTG GCTATAGAAC AAACAACTCT TAAGATGATG 
CAGACTGTGG GAGGCGGGCC TGCCCGCGCC TCTGGAGAGT ATATCTGTAA TCAGTGTGGT 
GCTAAGTACA CGTCCCTAGA CAGCTTTCAG ACTCACCTCA AAACCCATTT GGACACCGTG 
CTGCCAAAAC TGACCTGCCC TCAGTGTAAC AAAGAATTCC CCAACCAAGA GTCCTTGCTA 
AAGCACGTGA CCATCCACTT TATGATCACC TCAACCTACT ACATCTGTGA GAGCTGTGAC 
AAGCAGTTCA CCTCCGTGGA TGACCTCCAG AAGCACCTGC TGGACATGCA CACCTTCGTC 
TTCTTCCGCT GCACACTCTG CCAGGAAGTG TTCGACTCCA AGGTCTCTAT CCAGCTGCAC 
TTGGCCGTGA AGCACAGTAA CGAGAAGAAG GTGTACCGCT GCACGTCCTG CAACTGGGAC 
TTCCGCAACG AGACCGACCT GCAGCTGCAC GTGAAGCACA ACCACTTGGA GAACCAAGGC 
AAAGTGCACA AGTGCATCTT CTGCGGCGAG TCGTTTGGCA CCGAGGTGGA GCTGCAGTGC 
CACATCACCA CCCACAGCAA GAAGTACAAC TGCAGGTTCT GCAGCAAAGC CTTCCACGCC 
GTCATCCTGC TGGAGAAGCA CCTGCGGGAG AAACACTGTG TGTTTGAAAC CAAGACCCCC 
AACTGTGGCA CCAACGGGGC CTCGGAGCAA GTGCAGAAGG AGGAGGCTGA GCTGCAGACC 
TTGCTCACCA ACAGCCAGGA GTCCCATAAC AGCCATGATG GGAGTGAGGA GGACGTGGAC 
AGCTCTGAAC CCATGTATGG CTGTGACATC TGCGGGGCGG CCTACACCAT GGAGACGTTG 
CTGCAGAACC ACCAGCTCCG AGACCACAAC ATCAGGCCCG GAGAAAGCGC CATCGTGAAG 
AAGAAGGCGG AGCTCATTAA AGGGAATTAC AAGTGTAACG TTTGCTCGAG AACCTTCTTC 
TCCGAAAACG GGCTCCGTGA ACACATGCAG ACCCACTTAG GCCCGGTCAA ACACTACATG 
TGCCCTATCT GTGGAGAGCG CTTCCCTTCC CTGTTAACTC TCACAGAGCA CAAAGTCACA 
CACAGCAAGA GCCTGGATAC CGGAAACTGT CGTATTTGTA AGATGCCCCT GCAGAGCGAA 
GAGGAGTTTT TGGAGCACTG CCAGATGCAC CCTGACCTGA GGAATTCCCT GACTGGGTTT 
CGTTGTGTGG TGTGTATGCA GACTGTGACC TCCACCTTGG AACTCAAAAT CCACGGGACC 
TTCCACATGC AGAAGACGGG GAATGGGTCC TCCGTGCAGA CCACCGGGCG TGGCCAGCAC 
GTTCAGAAAC TGTATAAGTG CGCATCTTGC CTCAAAGAGT TCCGTTCCAA GCAAGATCTG 
GTGAAACTCG ACATCAACGG CCTGCCATAT GGTCTGTGTG CCGGCTGTGT GAATCTCAGT 
AAGAGCAGTA GCCCGGGCCT CAGTCTCCCT CCTGGTGCCA GCAGGCCAGG CTTGGGCCAG 
AATGAGAGTC TGAGCGCCAT GGAAGGAAAA GGCAAGGCGG GGGGACTGAA GACGCGCTGT 
TCAAGCTGCA ATGTCAAGTT TGAGTCTGAA AGCGAACTGC AGAACCACAT CCAGACGGTG 
CACCGGGAGC TTGTGCCAGA CGCCAACAGC ACACAGTTGA AAACGCCCCA AGTGTCACCC 
ATGCCCAGAA TCAGTCCCTC CCAGTCCGAT GAGAAGAAGA CCTACCAGTG TATCAAGTGT 
CAGATGGTTT TCTACAACGA GTGGGACATC CAGGTTCATG TGGCAAATCA CATGATTGAT 
GAAGGGCTGA ACCATGAGTG CAAACTCTGC AGCCAGACCT TTGACTCCCC CGCCAAACTT 
CAGTGTCACC TGATAGAGCA CAGCTTCGAA GGGATGGGTG GTACCTTCAA GTGCCCCGTC 
TGTTTCACAG TGTTTGTTCA AGCTAACAAG TTGCAACAGC ATATATTCTC TGCCCATGGA 
CAAGAAGACA AGATCTATGA CTGCACACAA TGCCCACAGA AGTTTTTCTT CCAAACAGAG 
CTACAGAATC ATACGATGAC TCAGCACAGC AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525883.1
CDS	27743..31018
Translation	MQVHERNKDGSQSGSRMEDWKMKDTQKCSQCEEGFDFPEDLQKHIAECHPECSPNEDRAALQCMYCHELFVEETSLMNHIEQVHGGEKKNSCSICSESFLTVEELYSHMDSHQQPESCNHSNSPSLVTVGYTSVSSTTPDSNLSVDSSTMVEAAPPIPKSRGRKRAAQQTSDMTGPSSKQAKVTYSCIYCNKQLFSSLAVLQIHLKTMHLDKPEQAHICQYCLEVLPSLYNLNEHLKQVHEAQDPGLIVSAMPAIVYQCNFCSEVVNDLNTLQEHIRCSHGFANPAAKDSNAFFCPHCYMGFLTDSSLEEHIRQVHCDLSGSRFGSPVLGTPKEPVVEVYSCSYCTNSPIFNSVLKLNKHIKENHKNIPLALNYIHNGKKSRALSPLSPVAIEQTTLKMMQTVGGGPARASGEYICNQCGAKYTSLDSFQTHLKTHLDTVLPKLTCPQCNKEFPNQESLLKHVTIHFMITSTYYICESCDKQFTSVDDLQKHLLDMHTFVFFRCTLCQEVFDSKVSIQLHLAVKHSNEKKVYRCTSCNWDFRNETDLQLHVKHNHLENQGKVHKCIFCGESFGTEVELQCHITTHSKKYNCRFCSKAFHAVILLEKHLREKHCVFETKTPNCGTNGASEQVQKEEAELQTLLTNSQESHNSHDGSEEDVDSSEPMYGCDICGAAYTMETLLQNHQLRDHNIRPGESAIVKKKAELIKGNYKCNVCSRTFFSENGLREHMQTHLGPVKHYMCPICGERFPSLLTLTEHKVTHSKSLDTGNCRICKMPLQSEEEFLEHCQMHPDLRNSLTGFRCVVCMQTVTSTLELKIHGTFHMQKTGNGSSVQTTGRGQHVQKLYKCASCLKEFRSKQDLVKLDINGLPYGLCAGCVNLSKSSSPGLSLPPGASRPGLGQNESLSAMEGKGKAGGLKTRCSSCNVKFESESELQNHIQTVHRELVPDANSTQLKTPQVSPMPRISPSQSDEKKTYQCIKCQMVFYNEWDIQVHVANHMIDEGLNHECKLCSQTFDSPAKLQCHLIEHSFEGMGGTFKCPVCFTVFVQANKLQQHIFSAHGQEDKIYDCTQCPQKFFFQTELQNHTMTQHSS

Target ORF information:

RefSeq Version	XM_006525820.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus zinc finger protein 521 (Zfp521), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525820.4

ORF Insert Sequence:

ATGCAGGTTC ATGAGCGGAA CAAGGACGGC TCCCAGTCTG GCTCCAGGAT GGAGGACTGG 
AAGATGAAGG ACACTCAGAA GTGCAGCCAG TGCGAGGAAG GCTTTGACTT CCCGGAAGAC 
CTCCAGAAGC ACATTGCAGA ATGCCACCCC GAGTGCTCCC CTAATGAGGA CCGAGCAGCC 
CTCCAGTGCA TGTACTGCCA CGAGCTGTTT GTGGAGGAGA CGTCCCTCAT GAACCACATC 
GAGCAGGTAC ACGGGGGTGA GAAGAAGAAC TCTTGCAGCA TTTGCTCAGA GAGCTTCCTC 
ACGGTCGAGG AGCTGTATAG CCACATGGAC AGTCACCAGC AGCCAGAGTC CTGCAATCAC 
AGCAACAGCC CTTCCCTGGT CACTGTGGGT TACACCTCAG TGTCCAGCAC GACTCCAGAC 
TCGAACCTCT CAGTGGACAG CTCGACCATG GTAGAGGCTG CACCACCGAT TCCAAAGAGC 
CGCGGGAGGA AGCGAGCTGC TCAGCAGACC TCCGATATGA CTGGCCCCTC TAGTAAGCAG 
GCGAAAGTCA CCTACAGCTG TATTTACTGC AACAAGCAGT TATTTTCCAG TCTCGCGGTT 
CTGCAGATTC ACTTGAAAAC TATGCATTTA GATAAGCCTG AGCAGGCTCA TATCTGTCAG 
TATTGCTTGG AGGTCTTACC CTCACTCTAT AACCTAAATG AACATCTTAA ACAAGTGCAC 
GAAGCTCAGG ACCCCGGCCT GATTGTTTCG GCCATGCCTG CCATTGTTTA CCAGTGCAAC 
TTCTGTTCCG AAGTTGTCAA TGACCTCAAC ACCCTTCAGG AGCACATCCG ATGTTCTCAT 
GGGTTTGCCA ATCCCGCGGC CAAGGACAGC AATGCGTTCT TTTGTCCCCA TTGTTACATG 
GGGTTTCTCA CTGACTCTTC ACTTGAAGAG CATATAAGAC AGGTCCATTG TGACCTCAGT 
GGCTCCCGGT TTGGGTCTCC TGTGCTTGGG ACTCCAAAAG AACCGGTGGT TGAAGTCTAC 
TCCTGTTCCT ATTGTACAAA TTCGCCAATA TTCAACAGTG TTCTTAAACT GAATAAGCAT 
ATTAAAGAGA ATCATAAAAA CATTCCCTTG GCCCTGAATT ATATTCACAA TGGGAAGAAA 
TCCCGGGCCT TGAGCCCTTT GTCCCCTGTG GCTATAGAAC AAACAACTCT TAAGATGATG 
CAGACTGTGG GAGGCGGGCC TGCCCGCGCC TCTGGAGAGT ATATCTGTAA TCAGTGTGGT 
GCTAAGTACA CGTCCCTAGA CAGCTTTCAG ACTCACCTCA AAACCCATTT GGACACCGTG 
CTGCCAAAAC TGACCTGCCC TCAGTGTAAC AAAGAATTCC CCAACCAAGA GTCCTTGCTA 
AAGCACGTGA CCATCCACTT TATGATCACC TCAACCTACT ACATCTGTGA GAGCTGTGAC 
AAGCAGTTCA CCTCCGTGGA TGACCTCCAG AAGCACCTGC TGGACATGCA CACCTTCGTC 
TTCTTCCGCT GCACACTCTG CCAGGAAGTG TTCGACTCCA AGGTCTCTAT CCAGCTGCAC 
TTGGCCGTGA AGCACAGTAA CGAGAAGAAG GTGTACCGCT GCACGTCCTG CAACTGGGAC 
TTCCGCAACG AGACCGACCT GCAGCTGCAC GTGAAGCACA ACCACTTGGA GAACCAAGGC 
AAAGTGCACA AGTGCATCTT CTGCGGCGAG TCGTTTGGCA CCGAGGTGGA GCTGCAGTGC 
CACATCACCA CCCACAGCAA GAAGTACAAC TGCAGGTTCT GCAGCAAAGC CTTCCACGCC 
GTCATCCTGC TGGAGAAGCA CCTGCGGGAG AAACACTGTG TGTTTGAAAC CAAGACCCCC 
AACTGTGGCA CCAACGGGGC CTCGGAGCAA GTGCAGAAGG AGGAGGCTGA GCTGCAGACC 
TTGCTCACCA ACAGCCAGGA GTCCCATAAC AGCCATGATG GGAGTGAGGA GGACGTGGAC 
AGCTCTGAAC CCATGTATGG CTGTGACATC TGCGGGGCGG CCTACACCAT GGAGACGTTG 
CTGCAGAACC ACCAGCTCCG AGACCACAAC ATCAGGCCCG GAGAAAGCGC CATCGTGAAG 
AAGAAGGCGG AGCTCATTAA AGGGAATTAC AAGTGTAACG TTTGCTCGAG AACCTTCTTC 
TCCGAAAACG GGCTCCGTGA ACACATGCAG ACCCACTTAG GCCCGGTCAA ACACTACATG 
TGCCCTATCT GTGGAGAGCG CTTCCCTTCC CTGTTAACTC TCACAGAGCA CAAAGTCACA 
CACAGCAAGA GCCTGGATAC CGGAAACTGT CGTATTTGTA AGATGCCCCT GCAGAGCGAA 
GAGGAGTTTT TGGAGCACTG CCAGATGCAC CCTGACCTGA GGAATTCCCT GACTGGGTTT 
CGTTGTGTGG TGTGTATGCA GACTGTGACC TCCACCTTGG AACTCAAAAT CCACGGGACC 
TTCCACATGC AGAAGACGGG GAATGGGTCC TCCGTGCAGA CCACCGGGCG TGGCCAGCAC 
GTTCAGAAAC TGTATAAGTG CGCATCTTGC CTCAAAGAGT TCCGTTCCAA GCAAGATCTG 
GTGAAACTCG ACATCAACGG CCTGCCATAT GGTCTGTGTG CCGGCTGTGT GAATCTCAGT 
AAGAGCAGTA GCCCGGGCCT CAGTCTCCCT CCTGGTGCCA GCAGGCCAGG CTTGGGCCAG 
AATGAGAGTC TGAGCGCCAT GGAAGGAAAA GGCAAGGCGG GGGGACTGAA GACGCGCTGT 
TCAAGCTGCA ATGTCAAGTT TGAGTCTGAA AGCGAACTGC AGAACCACAT CCAGACGGTG 
CACCGGGAGC TTGTGCCAGA CGCCAACAGC ACACAGTTGA AAACGCCCCA AGTGTCACCC 
ATGCCCAGAA TCAGTCCCTC CCAGTCCGAT GAGAAGAAGA CCTACCAGTG TATCAAGTGT 
CAGATGGTTT TCTACAACGA GTGGGACATC CAGGTTCATG TGGCAAATCA CATGATTGAT 
GAAGGGCTGA ACCATGAGTG CAAACTCTGC AGCCAGACCT TTGACTCCCC CGCCAAACTT 
CAGTGTCACC TGATAGAGCA CAGCTTCGAA GGGATGGGTG GTACCTTCAA GTGCCCCGTC 
TGTTTCACAG TGTTTGTTCA AGCTAACAAG TTGCAACAGC ATATATTCTC TGCCCATGGA 
CAAGAAGACA AGATCTATGA CTGCACACAA TGCCCACAGA AGTTTTTCTT CCAAACAGAG 
CTACAGAATC ATACGATGAC TCAGCACAGC AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Zfp521 ( NM_145492.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_663467.1 Mus musculus zinc finger protein 521 (Zfp521), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu11680
Clone ID Related Accession (Same CDS sequence)	NM_145492.4
Accession Version	NM_145492.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Mus musculus(house mouse)
Product	zinc finger protein 521 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC101651.6, AC104328.10, AC108404.8 and AC101682.8. On or before Apr 7, 2010 this sequence version replaced NM_181326.3, NM_145492.3. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.275419.1, AY147406.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTCGCC GCAAGCAAGC GAAACCGAGA TCCCTCAAAG ACCCCAACTG TAAACTTGAA 
GACAAGATTG AAGATGGGGA GGCAGTAGAC TGCAAGAAGA GGCCAGAAGA CGGGGAGGAG 
TTGGAGGAGG ACGCTGTGCA CAGCTGTGAC AGCTGCCTCC AGGTGTTTGA GTCACTGAGC 
GACATCACAG AGCACAAGAT CCATCAGTGC CAGCTGACAG ATGGCGTTGA CGTTGAGGAC 
GACCCCAGCT GCTCTTGGCC AGCCTCCTCG CCTTCCAGTA AGGACCAGAC TTCCCCCAGC 
CATGGAGAAG GGTGTGATTT CGGAGAGGAA GAAGGTGGCC CTGGACTGCC TTACCCATGC 
CAGTTCTGTG ACAAGTCCTT TAGCCGCCTC AGCTACCTAA AGCACCATGA ACAGAGCCAC 
AGTGACAAGC TGCCCTTCAA GTGCACCTAC TGCAGTCGGC TGTTCAAACA CAAGCGGAGC 
CGTGACCGCC ACATAAAGCT CCACACTGGG GATAAGAAGT ATCACTGTAG CGAGTGTGAC 
GCAGCCTTCT CCCGGAGCGA TCATCTCAAG ATCCACTTAA AGACTCACAC CTCCAACAAG 
CCATATAAAT GTGCCGTCTG CCGCAGAGGG TTCCTCTCCT CTAGCTCCTT GCACGGACAT 
ATGCAGGTTC ATGAGCGGAA CAAGGACGGC TCCCAGTCTG GCTCCAGGAT GGAGGACTGG 
AAGATGAAGG ACACTCAGAA GTGCAGCCAG TGCGAGGAAG GCTTTGACTT CCCGGAAGAC 
CTCCAGAAGC ACATTGCAGA ATGCCACCCC GAGTGCTCCC CTAATGAGGA CCGAGCAGCC 
CTCCAGTGCA TGTACTGCCA CGAGCTGTTT GTGGAGGAGA CGTCCCTCAT GAACCACATC 
GAGCAGGTAC ACGGGGGTGA GAAGAAGAAC TCTTGCAGCA TTTGCTCAGA GAGCTTCCTC 
ACGGTCGAGG AGCTGTATAG CCACATGGAC AGTCACCAGC AGCCAGAGTC CTGCAATCAC 
AGCAACAGCC CTTCCCTGGT CACTGTGGGT TACACCTCAG TGTCCAGCAC GACTCCAGAC 
TCGAACCTCT CAGTGGACAG CTCGACCATG GTAGAGGCTG CACCACCGAT TCCAAAGAGC 
CGCGGGAGGA AGCGAGCTGC TCAGCAGACC TCCGATATGA CTGGCCCCTC TAGTAAGCAG 
GCGAAAGTCA CCTACAGCTG TATTTACTGC AACAAGCAGT TATTTTCCAG TCTCGCGGTT 
CTGCAGATTC ACTTGAAAAC TATGCATTTA GATAAGCCTG AGCAGGCTCA TATCTGTCAG 
TATTGCTTGG AGGTCTTACC CTCACTCTAT AACCTAAATG AACATCTTAA ACAAGTGCAC 
GAAGCTCAGG ACCCCGGCCT GATTGTTTCG GCCATGCCTG CCATTGTTTA CCAGTGCAAC 
TTCTGTTCCG AAGTTGTCAA TGACCTCAAC ACCCTTCAGG AGCACATCCG ATGTTCTCAT 
GGGTTTGCCA ATCCCGCGGC CAAGGACAGC AATGCGTTCT TTTGTCCCCA TTGTTACATG 
GGGTTTCTCA CTGACTCTTC ACTTGAAGAG CATATAAGAC AGGTCCATTG TGACCTCAGT 
GGCTCCCGGT TTGGGTCTCC TGTGCTTGGG ACTCCAAAAG AACCGGTGGT TGAAGTCTAC 
TCCTGTTCCT ATTGTACAAA TTCGCCAATA TTCAACAGTG TTCTTAAACT GAATAAGCAT 
ATTAAAGAGA ATCATAAAAA CATTCCCTTG GCCCTGAATT ATATTCACAA TGGGAAGAAA 
TCCCGGGCCT TGAGCCCTTT GTCCCCTGTG GCTATAGAAC AAACAACTCT TAAGATGATG 
CAGACTGTGG GAGGCGGGCC TGCCCGCGCC TCTGGAGAGT ATATCTGTAA TCAGTGTGGT 
GCTAAGTACA CGTCCCTAGA CAGCTTTCAG ACTCACCTCA AAACCCATTT GGACACCGTG 
CTGCCAAAAC TGACCTGCCC TCAGTGTAAC AAAGAATTCC CCAACCAAGA GTCCTTGCTA 
AAGCACGTGA CCATCCACTT TATGATCACC TCAACCTACT ACATCTGTGA GAGCTGTGAC 
AAGCAGTTCA CCTCCGTGGA TGACCTCCAG AAGCACCTGC TGGACATGCA CACCTTCGTC 
TTCTTCCGCT GCACACTCTG CCAGGAAGTG TTCGACTCCA AGGTCTCTAT CCAGCTGCAC 
TTGGCCGTGA AGCACAGTAA CGAGAAGAAG GTGTACCGCT GCACGTCCTG CAACTGGGAC 
TTCCGCAACG AGACCGACCT GCAGCTGCAC GTGAAGCACA ACCACTTGGA GAACCAAGGC 
AAAGTGCACA AGTGCATCTT CTGCGGCGAG TCGTTTGGCA CCGAGGTGGA GCTGCAGTGC 
CACATCACCA CCCACAGCAA GAAGTACAAC TGCAGGTTCT GCAGCAAAGC CTTCCACGCC 
GTCATCCTGC TGGAGAAGCA CCTGCGGGAG AAACACTGTG TGTTTGAAAC CAAGACCCCC 
AACTGTGGCA CCAACGGGGC CTCGGAGCAA GTGCAGAAGG AGGAGGCTGA GCTGCAGACC 
TTGCTCACCA ACAGCCAGGA GTCCCATAAC AGCCATGATG GGAGTGAGGA GGACGTGGAC 
AGCTCTGAAC CCATGTATGG CTGTGACATC TGCGGGGCGG CCTACACCAT GGAGACGTTG 
CTGCAGAACC ACCAGCTCCG AGACCACAAC ATCAGGCCCG GAGAAAGCGC CATCGTGAAG 
AAGAAGGCGG AGCTCATTAA AGGGAATTAC AAGTGTAACG TTTGCTCGAG AACCTTCTTC 
TCCGAAAACG GGCTCCGTGA ACACATGCAG ACCCACTTAG GCCCGGTCAA ACACTACATG 
TGCCCTATCT GTGGAGAGCG CTTCCCTTCC CTGTTAACTC TCACAGAGCA CAAAGTCACA 
CACAGCAAGA GCCTGGATAC CGGAAACTGT CGTATTTGTA AGATGCCCCT GCAGAGCGAA 
GAGGAGTTTT TGGAGCACTG CCAGATGCAC CCTGACCTGA GGAATTCCCT GACTGGGTTT 
CGTTGTGTGG TGTGTATGCA GACTGTGACC TCCACCTTGG AACTCAAAAT CCACGGGACC 
TTCCACATGC AGAAGACGGG GAATGGGTCC TCCGTGCAGA CCACCGGGCG TGGCCAGCAC 
GTTCAGAAAC TGTATAAGTG CGCATCTTGC CTCAAAGAGT TCCGTTCCAA GCAAGATCTG 
GTGAAACTCG ACATCAACGG CCTGCCATAT GGTCTGTGTG CCGGCTGTGT GAATCTCAGT 
AAGAGCAGTA GCCCGGGCCT CAGTCTCCCT CCTGGTGCCA GCAGGCCAGG CTTGGGCCAG 
AATGAGAGTC TGAGCGCCAT GGAAGGAAAA GGCAAGGCGG GGGGACTGAA GACGCGCTGT 
TCAAGCTGCA ATGTCAAGTT TGAGTCTGAA AGCGAACTGC AGAACCACAT CCAGACGGTG 
CACCGGGAGC TTGTGCCAGA CGCCAACAGC ACACAGTTGA AAACGCCCCA AGTGTCACCC 
ATGCCCAGAA TCAGTCCCTC CCAGTCCGAT GAGAAGAAGA CCTACCAGTG TATCAAGTGT 
CAGATGGTTT TCTACAACGA GTGGGACATC CAGGTTCATG TGGCAAATCA CATGATTGAT 
GAAGGGCTGA ACCATGAGTG CAAACTCTGC AGCCAGACCT TTGACTCCCC CGCCAAACTT 
CAGTGTCACC TGATAGAGCA CAGCTTCGAA GGGATGGGTG GTACCTTCAA GTGCCCCGTC 
TGTTTCACAG TGTTTGTTCA AGCTAACAAG TTGCAACAGC ATATATTCTC TGCCCATGGA 
CAAGAAGACA AGATCTATGA CTGCACACAA TGCCCACAGA AGTTTTTCTT CCAAACAGAG 
CTACAGAATC ATACGATGAC TCAGCACAGC AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_663467.1
CDS	235..4170
Translation	MSRRKQAKPRSLKDPNCKLEDKIEDGEAVDCKKRPEDGEELEEDAVHSCDSCLQVFESLSDITEHKIHQCQLTDGVDVEDDPSCSWPASSPSSKDQTSPSHGEGCDFGEEEGGPGLPYPCQFCDKSFSRLSYLKHHEQSHSDKLPFKCTYCSRLFKHKRSRDRHIKLHTGDKKYHCSECDAAFSRSDHLKIHLKTHTSNKPYKCAVCRRGFLSSSSLHGHMQVHERNKDGSQSGSRMEDWKMKDTQKCSQCEEGFDFPEDLQKHIAECHPECSPNEDRAALQCMYCHELFVEETSLMNHIEQVHGGEKKNSCSICSESFLTVEELYSHMDSHQQPESCNHSNSPSLVTVGYTSVSSTTPDSNLSVDSSTMVEAAPPIPKSRGRKRAAQQTSDMTGPSSKQAKVTYSCIYCNKQLFSSLAVLQIHLKTMHLDKPEQAHICQYCLEVLPSLYNLNEHLKQVHEAQDPGLIVSAMPAIVYQCNFCSEVVNDLNTLQEHIRCSHGFANPAAKDSNAFFCPHCYMGFLTDSSLEEHIRQVHCDLSGSRFGSPVLGTPKEPVVEVYSCSYCTNSPIFNSVLKLNKHIKENHKNIPLALNYIHNGKKSRALSPLSPVAIEQTTLKMMQTVGGGPARASGEYICNQCGAKYTSLDSFQTHLKTHLDTVLPKLTCPQCNKEFPNQESLLKHVTIHFMITSTYYICESCDKQFTSVDDLQKHLLDMHTFVFFRCTLCQEVFDSKVSIQLHLAVKHSNEKKVYRCTSCNWDFRNETDLQLHVKHNHLENQGKVHKCIFCGESFGTEVELQCHITTHSKKYNCRFCSKAFHAVILLEKHLREKHCVFETKTPNCGTNGASEQVQKEEAELQTLLTNSQESHNSHDGSEEDVDSSEPMYGCDICGAAYTMETLLQNHQLRDHNIRPGESAIVKKKAELIKGNYKCNVCSRTFFSENGLREHMQTHLGPVKHYMCPICGERFPSLLTLTEHKVTHSKSLDTGNCRICKMPLQSEEEFLEHCQMHPDLRNSLTGFRCVVCMQTVTSTLELKIHGTFHMQKTGNGSSVQTTGRGQHVQKLYKCASCLKEFRSKQDLVKLDINGLPYGLCAGCVNLSKSSSPGLSLPPGASRPGLGQNESLSAMEGKGKAGGLKTRCSSCNVKFESESELQNHIQTVHRELVPDANSTQLKTPQVSPMPRISPSQSDEKKTYQCIKCQMVFYNEWDIQVHVANHMIDEGLNHECKLCSQTFDSPAKLQCHLIEHSFEGMGGTFKCPVCFTVFVQANKLQQHIFSAHGQEDKIYDCTQCPQKFFFQTELQNHTMTQHSS

Target ORF information:

RefSeq Version	NM_145492.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus zinc finger protein 521 (Zfp521), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145492.4

ORF Insert Sequence:

ATGTCTCGCC GCAAGCAAGC GAAACCGAGA TCCCTCAAAG ACCCCAACTG TAAACTTGAA 
GACAAGATTG AAGATGGGGA GGCAGTAGAC TGCAAGAAGA GGCCAGAAGA CGGGGAGGAG 
TTGGAGGAGG ACGCTGTGCA CAGCTGTGAC AGCTGCCTCC AGGTGTTTGA GTCACTGAGC 
GACATCACAG AGCACAAGAT CCATCAGTGC CAGCTGACAG ATGGCGTTGA CGTTGAGGAC 
GACCCCAGCT GCTCTTGGCC AGCCTCCTCG CCTTCCAGTA AGGACCAGAC TTCCCCCAGC 
CATGGAGAAG GGTGTGATTT CGGAGAGGAA GAAGGTGGCC CTGGACTGCC TTACCCATGC 
CAGTTCTGTG ACAAGTCCTT TAGCCGCCTC AGCTACCTAA AGCACCATGA ACAGAGCCAC 
AGTGACAAGC TGCCCTTCAA GTGCACCTAC TGCAGTCGGC TGTTCAAACA CAAGCGGAGC 
CGTGACCGCC ACATAAAGCT CCACACTGGG GATAAGAAGT ATCACTGTAG CGAGTGTGAC 
GCAGCCTTCT CCCGGAGCGA TCATCTCAAG ATCCACTTAA AGACTCACAC CTCCAACAAG 
CCATATAAAT GTGCCGTCTG CCGCAGAGGG TTCCTCTCCT CTAGCTCCTT GCACGGACAT 
ATGCAGGTTC ATGAGCGGAA CAAGGACGGC TCCCAGTCTG GCTCCAGGAT GGAGGACTGG 
AAGATGAAGG ACACTCAGAA GTGCAGCCAG TGCGAGGAAG GCTTTGACTT CCCGGAAGAC 
CTCCAGAAGC ACATTGCAGA ATGCCACCCC GAGTGCTCCC CTAATGAGGA CCGAGCAGCC 
CTCCAGTGCA TGTACTGCCA CGAGCTGTTT GTGGAGGAGA CGTCCCTCAT GAACCACATC 
GAGCAGGTAC ACGGGGGTGA GAAGAAGAAC TCTTGCAGCA TTTGCTCAGA GAGCTTCCTC 
ACGGTCGAGG AGCTGTATAG CCACATGGAC AGTCACCAGC AGCCAGAGTC CTGCAATCAC 
AGCAACAGCC CTTCCCTGGT CACTGTGGGT TACACCTCAG TGTCCAGCAC GACTCCAGAC 
TCGAACCTCT CAGTGGACAG CTCGACCATG GTAGAGGCTG CACCACCGAT TCCAAAGAGC 
CGCGGGAGGA AGCGAGCTGC TCAGCAGACC TCCGATATGA CTGGCCCCTC TAGTAAGCAG 
GCGAAAGTCA CCTACAGCTG TATTTACTGC AACAAGCAGT TATTTTCCAG TCTCGCGGTT 
CTGCAGATTC ACTTGAAAAC TATGCATTTA GATAAGCCTG AGCAGGCTCA TATCTGTCAG 
TATTGCTTGG AGGTCTTACC CTCACTCTAT AACCTAAATG AACATCTTAA ACAAGTGCAC 
GAAGCTCAGG ACCCCGGCCT GATTGTTTCG GCCATGCCTG CCATTGTTTA CCAGTGCAAC 
TTCTGTTCCG AAGTTGTCAA TGACCTCAAC ACCCTTCAGG AGCACATCCG ATGTTCTCAT 
GGGTTTGCCA ATCCCGCGGC CAAGGACAGC AATGCGTTCT TTTGTCCCCA TTGTTACATG 
GGGTTTCTCA CTGACTCTTC ACTTGAAGAG CATATAAGAC AGGTCCATTG TGACCTCAGT 
GGCTCCCGGT TTGGGTCTCC TGTGCTTGGG ACTCCAAAAG AACCGGTGGT TGAAGTCTAC 
TCCTGTTCCT ATTGTACAAA TTCGCCAATA TTCAACAGTG TTCTTAAACT GAATAAGCAT 
ATTAAAGAGA ATCATAAAAA CATTCCCTTG GCCCTGAATT ATATTCACAA TGGGAAGAAA 
TCCCGGGCCT TGAGCCCTTT GTCCCCTGTG GCTATAGAAC AAACAACTCT TAAGATGATG 
CAGACTGTGG GAGGCGGGCC TGCCCGCGCC TCTGGAGAGT ATATCTGTAA TCAGTGTGGT 
GCTAAGTACA CGTCCCTAGA CAGCTTTCAG ACTCACCTCA AAACCCATTT GGACACCGTG 
CTGCCAAAAC TGACCTGCCC TCAGTGTAAC AAAGAATTCC CCAACCAAGA GTCCTTGCTA 
AAGCACGTGA CCATCCACTT TATGATCACC TCAACCTACT ACATCTGTGA GAGCTGTGAC 
AAGCAGTTCA CCTCCGTGGA TGACCTCCAG AAGCACCTGC TGGACATGCA CACCTTCGTC 
TTCTTCCGCT GCACACTCTG CCAGGAAGTG TTCGACTCCA AGGTCTCTAT CCAGCTGCAC 
TTGGCCGTGA AGCACAGTAA CGAGAAGAAG GTGTACCGCT GCACGTCCTG CAACTGGGAC 
TTCCGCAACG AGACCGACCT GCAGCTGCAC GTGAAGCACA ACCACTTGGA GAACCAAGGC 
AAAGTGCACA AGTGCATCTT CTGCGGCGAG TCGTTTGGCA CCGAGGTGGA GCTGCAGTGC 
CACATCACCA CCCACAGCAA GAAGTACAAC TGCAGGTTCT GCAGCAAAGC CTTCCACGCC 
GTCATCCTGC TGGAGAAGCA CCTGCGGGAG AAACACTGTG TGTTTGAAAC CAAGACCCCC 
AACTGTGGCA CCAACGGGGC CTCGGAGCAA GTGCAGAAGG AGGAGGCTGA GCTGCAGACC 
TTGCTCACCA ACAGCCAGGA GTCCCATAAC AGCCATGATG GGAGTGAGGA GGACGTGGAC 
AGCTCTGAAC CCATGTATGG CTGTGACATC TGCGGGGCGG CCTACACCAT GGAGACGTTG 
CTGCAGAACC ACCAGCTCCG AGACCACAAC ATCAGGCCCG GAGAAAGCGC CATCGTGAAG 
AAGAAGGCGG AGCTCATTAA AGGGAATTAC AAGTGTAACG TTTGCTCGAG AACCTTCTTC 
TCCGAAAACG GGCTCCGTGA ACACATGCAG ACCCACTTAG GCCCGGTCAA ACACTACATG 
TGCCCTATCT GTGGAGAGCG CTTCCCTTCC CTGTTAACTC TCACAGAGCA CAAAGTCACA 
CACAGCAAGA GCCTGGATAC CGGAAACTGT CGTATTTGTA AGATGCCCCT GCAGAGCGAA 
GAGGAGTTTT TGGAGCACTG CCAGATGCAC CCTGACCTGA GGAATTCCCT GACTGGGTTT 
CGTTGTGTGG TGTGTATGCA GACTGTGACC TCCACCTTGG AACTCAAAAT CCACGGGACC 
TTCCACATGC AGAAGACGGG GAATGGGTCC TCCGTGCAGA CCACCGGGCG TGGCCAGCAC 
GTTCAGAAAC TGTATAAGTG CGCATCTTGC CTCAAAGAGT TCCGTTCCAA GCAAGATCTG 
GTGAAACTCG ACATCAACGG CCTGCCATAT GGTCTGTGTG CCGGCTGTGT GAATCTCAGT 
AAGAGCAGTA GCCCGGGCCT CAGTCTCCCT CCTGGTGCCA GCAGGCCAGG CTTGGGCCAG 
AATGAGAGTC TGAGCGCCAT GGAAGGAAAA GGCAAGGCGG GGGGACTGAA GACGCGCTGT 
TCAAGCTGCA ATGTCAAGTT TGAGTCTGAA AGCGAACTGC AGAACCACAT CCAGACGGTG 
CACCGGGAGC TTGTGCCAGA CGCCAACAGC ACACAGTTGA AAACGCCCCA AGTGTCACCC 
ATGCCCAGAA TCAGTCCCTC CCAGTCCGAT GAGAAGAAGA CCTACCAGTG TATCAAGTGT 
CAGATGGTTT TCTACAACGA GTGGGACATC CAGGTTCATG TGGCAAATCA CATGATTGAT 
GAAGGGCTGA ACCATGAGTG CAAACTCTGC AGCCAGACCT TTGACTCCCC CGCCAAACTT 
CAGTGTCACC TGATAGAGCA CAGCTTCGAA GGGATGGGTG GTACCTTCAA GTGCCCCGTC 
TGTTTCACAG TGTTTGTTCA AGCTAACAAG TTGCAACAGC ATATATTCTC TGCCCATGGA 
CAAGAAGACA AGATCTATGA CTGCACACAA TGCCCACAGA AGTTTTTCTT CCAAACAGAG 
CTACAGAATC ATACGATGAC TCAGCACAGC AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Zfp521 ( NM_001360804.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001347733.1 Mus musculus zinc finger protein 521 (Zfp521), transcript variant 2, mRNA.

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CloneID	OMu73236
Clone ID Related Accession (Same CDS sequence)	XM_011246894.1 , NM_001360804.1
Accession Version	NM_001360804.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3933bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Mus musculus(house mouse)
Product	zinc finger protein 521 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC101651.6, AC104328.10, AC108404.8 and AC101682.8. On Feb 14, 2018 this sequence version replaced XM_011246894.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660815.279809.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTCGCC GCAAGCAAGC GAAACCGAGA TCCCTCAAAG ACCCCAACTG TAAACTTGAA 
GACAAGATTG AAGATGGGGA GGCAGTAGAC TGCAAGAAGA GGCCAGAAGA CGGGGAGGAG 
TTGGAGGAGG ACGCTGTGCA CAGCTGTGAC AGCTGCCTCC AGGTGTTTGA GTCACTGAGC 
GACATCACAG AGCACAAGAT CCATCAGTGC CAGCTGACAG ATGGCGTTGA CGTTGAGGAC 
GACCCCAGCT GCTCTTGGCC AGCCTCCTCG CCTTCCAGTA AGGACCAGAC TTCCCCCAGC 
CATGGAGAAG GGTGTGATTT CGGAGAGGAA GAAGGTGGCC CTGGACTGCC TTACCCATGC 
CAGTTCTGTG ACAAGTCCTT TAGCCGCCTC AGCTACCTAA AGCACCATGA ACAGAGCCAC 
AGTGACAAGC TGCCCTTCAA GTGCACCTAC TGCAGTCGGC TGTTCAAACA CAAGCGGAGC 
CGTGACCGCC ACATAAAGCT CCACACTGGG GATAAGAAGT ATCACTGTAG CGAGTGTGAC 
GCAGCCTTCT CCCGGAGCGA TCATCTCAAG ATCCACTTAA AGACTCACAC CTCCAACAAG 
CCATATAAAT GTGCCGTCTG CCGCAGAGGG TTCCTCTCCT CTAGCTCCTT GCACGGACAT 
ATGCAGGTTC ATGAGCGGAA CAAGGACGGC TCCCAGTCTG GCTCCAGGAT GGAGGACTGG 
AAGATGAAGG ACACTCAGAA GTGCAGCCAG TGCGAGGAAG GCTTTGACTT CCCGGAAGAC 
CTCCAGAAGC ACATTGCAGA ATGCCACCCC GAGTGCTCCC CTAATGAGGA CCGAGCAGCC 
CTCCAGTGCA TGTACTGCCA CGAGCTGTTT GTGGAGGAGA CGTCCCTCAT GAACCACATC 
GAGCAGGTAC ACGGGGGTGA GAAGAAGAAC TCTTGCAGCA TTTGCTCAGA GAGCTTCCTC 
ACGGTCGAGG AGCTGTATAG CCACATGGAC AGTCACCAGC AGCCAGAGTC CTGCAATCAC 
AGCAACAGCC CTTCCCTGGT CACTGTGGGT TACACCTCAG TGTCCAGCAC GACTCCAGAC 
TCGAACCTCT CAGTGGACAG CTCGACCATG GTAGAGGCTG CACCACCGAT TCCAAAGAGC 
CGCGGGAGGA AGCGAGCTGC TCAGCAGACC TCCGATATGA CTGGCCCCTC TAGTAAGCAG 
GCGAAAGTCA CCTACAGCTG TATTTACTGC AACAAGCAGT TATTTTCCAG TCTCGCGGTT 
CTGCAGATTC ACTTGAAAAC TATGCATTTA GATAAGCCTG AGCAGGCTCA TATCTGTCAG 
TATTGCTTGG AGGTCTTACC CTCACTCTAT AACCTAAATG AACATCTTAA ACAAGTGCAC 
GAAGCTCAGG ACCCCGGCCT GATTGTTTCG GCCATGCCTG CCATTGTTTA CCAGTGCAAC 
TTCTGTTCCG AAGTTGTCAA TGACCTCAAC ACCCTTCAGG AGCACATCCG ATGTTCTCAT 
GGGTTTGCCA ATCCCGCGGC CAAGGACAGC AATGCGTTCT TTTGTCCCCA TTGTTACATG 
GGGTTTCTCA CTGACTCTTC ACTTGAAGAG CATATAAGAC AGGTCCATTG TGACCTCAGT 
GGCTCCCGGT TTGGGTCTCC TGTGCTTGGG ACTCCAAAAG AACCGGTGGT TGAAGTCTAC 
TCCTGTTCCT ATTGTACAAA TTCGCCAATA TTCAACAGTG TTCTTAAACT GAATAAGCAT 
ATTAAAGAGA ATCATAAAAA CATTCCCTTG GCCCTGAATT ATATTCACAA TGGGAAGAAA 
TCCCGGGCCT TGAGCCCTTT GTCCCCTGTG GCTATAGAAC AAACAACTCT TAAGATGATG 
CAGACTGTGG GAGGCGGGCC TGCCCGCGCC TCTGGAGAGT ATATCTGTAA TCAGTGTGGT 
GCTAAGTACA CGTCCCTAGA CAGCTTTCAG ACTCACCTCA AAACCCATTT GGACACCGTG 
CTGCCAAAAC TGACCTGCCC TCAGTGTAAC AAAGAATTCC CCAACCAAGA GTCCTTGCTA 
AAGCACGTGA CCATCCACTT TATGATCACC TCAACCTACT ACATCTGTGA GAGCTGTGAC 
AAGCAGTTCA CCTCCGTGGA TGACCTCCAG AAGCACCTGC TGGACATGCA CACCTTCGTC 
TTCTTCCGCT GCACACTCTG CCAGGAAGTG TTCGACTCCA AGGTCTCTAT CCAGCTGCAC 
TTGGCCGTGA AGCACAGTAA CGAGAAGAAG GTGTACCGCT GCACGTCCTG CAACTGGGAC 
TTCCGCAACG AGACCGACCT GCAGCTGCAC GTGAAGCACA ACCACTTGGA GAACCAAGGC 
AAAGTGCACA AGTGCATCTT CTGCGGCGAG TCGTTTGGCA CCGAGGTGGA GCTGCAGTGC 
CACATCACCA CCCACAGCAA GAAGTACAAC TGCAGGTTCT GCAGCAAAGC CTTCCACGCC 
GTCATCCTGC TGGAGAAGCA CCTGCGGGAG AAACACTGTG TGTTTGAAAC CAAGACCCCC 
AACTGTGGCA CCAACGGGGC CTCGGAGCAA GTGCAGAAGG AGGAGGCTGA GCTGCAGACC 
TTGCTCACCA ACAGCCAGGA GTCCCATAAC AGCCATGATG GGAGTGAGGA GGACGTGGAC 
AGCTCTGAAC CCATGTATGG CTGTGACATC TGCGGGGCGG CCTACACCAT GGAGACGTTG 
CTGCAGAACC ACCAGCTCCG AGACCACAAC ATCAGGCCCG GAGAAAGCGC CATCGTGAAG 
AAGAAGGCGG AGCTCATTAA AGGGAATTAC AAGTGTAACG TTTGCTCGAG AACCTTCTTC 
TCCGAAAACG GGCTCCGTGA ACACATGCAG ACCCACTTAG GCCCGGTCAA ACACTACATG 
TGCCCTATCT GTGGAGAGCG CTTCCCTTCC CTGTTAACTC TCACAGAGCA CAAAGTCACA 
CACAGCAAGA GCCTGGATAC CGGAAACTGT CGTATTTGTA AGATGCCCCT GCAGAGCGAA 
GAGGAGTTTT TGGAGCACTG CCAGATGCAC CCTGACCTGA GGAATTCCCT GACTGGGTTT 
CGTTGTGTGG TGTGTATGCA GACTGTGACC TCCACCTTGG AACTCAAAAT CCACGGGACC 
TTCCACATGC AGAAGACGGG GAATGGGTCC TCCGTGCAGA CCACCGGGCG TGGCCAGCAC 
GTTCAGAAAC TGTATAAGTG CGCATCTTGC CTCAAAGAGT TCCGTTCCAA GCAAGATCTG 
GTGAAACTCG ACATCAACGG CCTGCCATAT GGTCTGTGTG CCGGCTGTGT GAATCTCAGT 
AAGAGCAGTA GCCCGGGCCT CAGTCTCCCT CCTGGTGCCA GCAGGCCAGG CTTGGGCCAG 
AATGAGAGTC TGAGCGCCAT GGAAGGAAAA GGCAAGGCGG GGGGACTGAA GACGCGCTGT 
TCAAGCTGCA ATGTCAAGTT TGAGTCTGAA AGCGAACTGC AGAACCACAT CCAGACGGTG 
CACCGGGAGC TTGTGCCAGA CGCCAACAGC ACACAGTTGA AAACGCCCCA AGTGTCACCC 
ATGCCCAGAA TCAGTCCCTC CCAGTCCGAT GAGAAGACCT ACCAGTGTAT CAAGTGTCAG 
ATGGTTTTCT ACAACGAGTG GGACATCCAG GTTCATGTGG CAAATCACAT GATTGATGAA 
GGGCTGAACC ATGAGTGCAA ACTCTGCAGC CAGACCTTTG ACTCCCCCGC CAAACTTCAG 
TGTCACCTGA TAGAGCACAG CTTCGAAGGG ATGGGTGGTA CCTTCAAGTG CCCCGTCTGT 
TTCACAGTGT TTGTTCAAGC TAACAAGTTG CAACAGCATA TATTCTCTGC CCATGGACAA 
GAAGACAAGA TCTATGACTG CACACAATGC CCACAGAAGT TTTTCTTCCA AACAGAGCTA 
CAGAATCATA CGATGACTCA GCACAGCAGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001347733.1
CDS	235..4167
Translation	MSRRKQAKPRSLKDPNCKLEDKIEDGEAVDCKKRPEDGEELEEDAVHSCDSCLQVFESLSDITEHKIHQCQLTDGVDVEDDPSCSWPASSPSSKDQTSPSHGEGCDFGEEEGGPGLPYPCQFCDKSFSRLSYLKHHEQSHSDKLPFKCTYCSRLFKHKRSRDRHIKLHTGDKKYHCSECDAAFSRSDHLKIHLKTHTSNKPYKCAVCRRGFLSSSSLHGHMQVHERNKDGSQSGSRMEDWKMKDTQKCSQCEEGFDFPEDLQKHIAECHPECSPNEDRAALQCMYCHELFVEETSLMNHIEQVHGGEKKNSCSICSESFLTVEELYSHMDSHQQPESCNHSNSPSLVTVGYTSVSSTTPDSNLSVDSSTMVEAAPPIPKSRGRKRAAQQTSDMTGPSSKQAKVTYSCIYCNKQLFSSLAVLQIHLKTMHLDKPEQAHICQYCLEVLPSLYNLNEHLKQVHEAQDPGLIVSAMPAIVYQCNFCSEVVNDLNTLQEHIRCSHGFANPAAKDSNAFFCPHCYMGFLTDSSLEEHIRQVHCDLSGSRFGSPVLGTPKEPVVEVYSCSYCTNSPIFNSVLKLNKHIKENHKNIPLALNYIHNGKKSRALSPLSPVAIEQTTLKMMQTVGGGPARASGEYICNQCGAKYTSLDSFQTHLKTHLDTVLPKLTCPQCNKEFPNQESLLKHVTIHFMITSTYYICESCDKQFTSVDDLQKHLLDMHTFVFFRCTLCQEVFDSKVSIQLHLAVKHSNEKKVYRCTSCNWDFRNETDLQLHVKHNHLENQGKVHKCIFCGESFGTEVELQCHITTHSKKYNCRFCSKAFHAVILLEKHLREKHCVFETKTPNCGTNGASEQVQKEEAELQTLLTNSQESHNSHDGSEEDVDSSEPMYGCDICGAAYTMETLLQNHQLRDHNIRPGESAIVKKKAELIKGNYKCNVCSRTFFSENGLREHMQTHLGPVKHYMCPICGERFPSLLTLTEHKVTHSKSLDTGNCRICKMPLQSEEEFLEHCQMHPDLRNSLTGFRCVVCMQTVTSTLELKIHGTFHMQKTGNGSSVQTTGRGQHVQKLYKCASCLKEFRSKQDLVKLDINGLPYGLCAGCVNLSKSSSPGLSLPPGASRPGLGQNESLSAMEGKGKAGGLKTRCSSCNVKFESESELQNHIQTVHRELVPDANSTQLKTPQVSPMPRISPSQSDEKTYQCIKCQMVFYNEWDIQVHVANHMIDEGLNHECKLCSQTFDSPAKLQCHLIEHSFEGMGGTFKCPVCFTVFVQANKLQQHIFSAHGQEDKIYDCTQCPQKFFFQTELQNHTMTQHSS

Target ORF information:

RefSeq Version	NM_001360804.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus zinc finger protein 521 (Zfp521), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001360804.1

ORF Insert Sequence:

ATGTCTCGCC GCAAGCAAGC GAAACCGAGA TCCCTCAAAG ACCCCAACTG TAAACTTGAA 
GACAAGATTG AAGATGGGGA GGCAGTAGAC TGCAAGAAGA GGCCAGAAGA CGGGGAGGAG 
TTGGAGGAGG ACGCTGTGCA CAGCTGTGAC AGCTGCCTCC AGGTGTTTGA GTCACTGAGC 
GACATCACAG AGCACAAGAT CCATCAGTGC CAGCTGACAG ATGGCGTTGA CGTTGAGGAC 
GACCCCAGCT GCTCTTGGCC AGCCTCCTCG CCTTCCAGTA AGGACCAGAC TTCCCCCAGC 
CATGGAGAAG GGTGTGATTT CGGAGAGGAA GAAGGTGGCC CTGGACTGCC TTACCCATGC 
CAGTTCTGTG ACAAGTCCTT TAGCCGCCTC AGCTACCTAA AGCACCATGA ACAGAGCCAC 
AGTGACAAGC TGCCCTTCAA GTGCACCTAC TGCAGTCGGC TGTTCAAACA CAAGCGGAGC 
CGTGACCGCC ACATAAAGCT CCACACTGGG GATAAGAAGT ATCACTGTAG CGAGTGTGAC 
GCAGCCTTCT CCCGGAGCGA TCATCTCAAG ATCCACTTAA AGACTCACAC CTCCAACAAG 
CCATATAAAT GTGCCGTCTG CCGCAGAGGG TTCCTCTCCT CTAGCTCCTT GCACGGACAT 
ATGCAGGTTC ATGAGCGGAA CAAGGACGGC TCCCAGTCTG GCTCCAGGAT GGAGGACTGG 
AAGATGAAGG ACACTCAGAA GTGCAGCCAG TGCGAGGAAG GCTTTGACTT CCCGGAAGAC 
CTCCAGAAGC ACATTGCAGA ATGCCACCCC GAGTGCTCCC CTAATGAGGA CCGAGCAGCC 
CTCCAGTGCA TGTACTGCCA CGAGCTGTTT GTGGAGGAGA CGTCCCTCAT GAACCACATC 
GAGCAGGTAC ACGGGGGTGA GAAGAAGAAC TCTTGCAGCA TTTGCTCAGA GAGCTTCCTC 
ACGGTCGAGG AGCTGTATAG CCACATGGAC AGTCACCAGC AGCCAGAGTC CTGCAATCAC 
AGCAACAGCC CTTCCCTGGT CACTGTGGGT TACACCTCAG TGTCCAGCAC GACTCCAGAC 
TCGAACCTCT CAGTGGACAG CTCGACCATG GTAGAGGCTG CACCACCGAT TCCAAAGAGC 
CGCGGGAGGA AGCGAGCTGC TCAGCAGACC TCCGATATGA CTGGCCCCTC TAGTAAGCAG 
GCGAAAGTCA CCTACAGCTG TATTTACTGC AACAAGCAGT TATTTTCCAG TCTCGCGGTT 
CTGCAGATTC ACTTGAAAAC TATGCATTTA GATAAGCCTG AGCAGGCTCA TATCTGTCAG 
TATTGCTTGG AGGTCTTACC CTCACTCTAT AACCTAAATG AACATCTTAA ACAAGTGCAC 
GAAGCTCAGG ACCCCGGCCT GATTGTTTCG GCCATGCCTG CCATTGTTTA CCAGTGCAAC 
TTCTGTTCCG AAGTTGTCAA TGACCTCAAC ACCCTTCAGG AGCACATCCG ATGTTCTCAT 
GGGTTTGCCA ATCCCGCGGC CAAGGACAGC AATGCGTTCT TTTGTCCCCA TTGTTACATG 
GGGTTTCTCA CTGACTCTTC ACTTGAAGAG CATATAAGAC AGGTCCATTG TGACCTCAGT 
GGCTCCCGGT TTGGGTCTCC TGTGCTTGGG ACTCCAAAAG AACCGGTGGT TGAAGTCTAC 
TCCTGTTCCT ATTGTACAAA TTCGCCAATA TTCAACAGTG TTCTTAAACT GAATAAGCAT 
ATTAAAGAGA ATCATAAAAA CATTCCCTTG GCCCTGAATT ATATTCACAA TGGGAAGAAA 
TCCCGGGCCT TGAGCCCTTT GTCCCCTGTG GCTATAGAAC AAACAACTCT TAAGATGATG 
CAGACTGTGG GAGGCGGGCC TGCCCGCGCC TCTGGAGAGT ATATCTGTAA TCAGTGTGGT 
GCTAAGTACA CGTCCCTAGA CAGCTTTCAG ACTCACCTCA AAACCCATTT GGACACCGTG 
CTGCCAAAAC TGACCTGCCC TCAGTGTAAC AAAGAATTCC CCAACCAAGA GTCCTTGCTA 
AAGCACGTGA CCATCCACTT TATGATCACC TCAACCTACT ACATCTGTGA GAGCTGTGAC 
AAGCAGTTCA CCTCCGTGGA TGACCTCCAG AAGCACCTGC TGGACATGCA CACCTTCGTC 
TTCTTCCGCT GCACACTCTG CCAGGAAGTG TTCGACTCCA AGGTCTCTAT CCAGCTGCAC 
TTGGCCGTGA AGCACAGTAA CGAGAAGAAG GTGTACCGCT GCACGTCCTG CAACTGGGAC 
TTCCGCAACG AGACCGACCT GCAGCTGCAC GTGAAGCACA ACCACTTGGA GAACCAAGGC 
AAAGTGCACA AGTGCATCTT CTGCGGCGAG TCGTTTGGCA CCGAGGTGGA GCTGCAGTGC 
CACATCACCA CCCACAGCAA GAAGTACAAC TGCAGGTTCT GCAGCAAAGC CTTCCACGCC 
GTCATCCTGC TGGAGAAGCA CCTGCGGGAG AAACACTGTG TGTTTGAAAC CAAGACCCCC 
AACTGTGGCA CCAACGGGGC CTCGGAGCAA GTGCAGAAGG AGGAGGCTGA GCTGCAGACC 
TTGCTCACCA ACAGCCAGGA GTCCCATAAC AGCCATGATG GGAGTGAGGA GGACGTGGAC 
AGCTCTGAAC CCATGTATGG CTGTGACATC TGCGGGGCGG CCTACACCAT GGAGACGTTG 
CTGCAGAACC ACCAGCTCCG AGACCACAAC ATCAGGCCCG GAGAAAGCGC CATCGTGAAG 
AAGAAGGCGG AGCTCATTAA AGGGAATTAC AAGTGTAACG TTTGCTCGAG AACCTTCTTC 
TCCGAAAACG GGCTCCGTGA ACACATGCAG ACCCACTTAG GCCCGGTCAA ACACTACATG 
TGCCCTATCT GTGGAGAGCG CTTCCCTTCC CTGTTAACTC TCACAGAGCA CAAAGTCACA 
CACAGCAAGA GCCTGGATAC CGGAAACTGT CGTATTTGTA AGATGCCCCT GCAGAGCGAA 
GAGGAGTTTT TGGAGCACTG CCAGATGCAC CCTGACCTGA GGAATTCCCT GACTGGGTTT 
CGTTGTGTGG TGTGTATGCA GACTGTGACC TCCACCTTGG AACTCAAAAT CCACGGGACC 
TTCCACATGC AGAAGACGGG GAATGGGTCC TCCGTGCAGA CCACCGGGCG TGGCCAGCAC 
GTTCAGAAAC TGTATAAGTG CGCATCTTGC CTCAAAGAGT TCCGTTCCAA GCAAGATCTG 
GTGAAACTCG ACATCAACGG CCTGCCATAT GGTCTGTGTG CCGGCTGTGT GAATCTCAGT 
AAGAGCAGTA GCCCGGGCCT CAGTCTCCCT CCTGGTGCCA GCAGGCCAGG CTTGGGCCAG 
AATGAGAGTC TGAGCGCCAT GGAAGGAAAA GGCAAGGCGG GGGGACTGAA GACGCGCTGT 
TCAAGCTGCA ATGTCAAGTT TGAGTCTGAA AGCGAACTGC AGAACCACAT CCAGACGGTG 
CACCGGGAGC TTGTGCCAGA CGCCAACAGC ACACAGTTGA AAACGCCCCA AGTGTCACCC 
ATGCCCAGAA TCAGTCCCTC CCAGTCCGAT GAGAAGACCT ACCAGTGTAT CAAGTGTCAG 
ATGGTTTTCT ACAACGAGTG GGACATCCAG GTTCATGTGG CAAATCACAT GATTGATGAA 
GGGCTGAACC ATGAGTGCAA ACTCTGCAGC CAGACCTTTG ACTCCCCCGC CAAACTTCAG 
TGTCACCTGA TAGAGCACAG CTTCGAAGGG ATGGGTGGTA CCTTCAAGTG CCCCGTCTGT 
TTCACAGTGT TTGTTCAAGC TAACAAGTTG CAACAGCATA TATTCTCTGC CCATGGACAA 
GAAGACAAGA TCTATGACTG CACACAATGC CCACAGAAGT TTTTCTTCCA AACAGAGCTA 
CAGAATCATA CGATGACTCA GCACAGCAGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Zfp521 ( XM_006525820.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525883.1 Mus musculus zinc finger protein 521 (Zfp521), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu54502
Clone ID Related Accession (Same CDS sequence)	XM_006525820.4 , XM_006525820.3 , XM_006525819.3
Accession Version	XM_006525820.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	zinc finger protein 521 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006525820.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGTTC ATGAGCGGAA CAAGGACGGC TCCCAGTCTG GCTCCAGGAT GGAGGACTGG 
AAGATGAAGG ACACTCAGAA GTGCAGCCAG TGCGAGGAAG GCTTTGACTT CCCGGAAGAC 
CTCCAGAAGC ACATTGCAGA ATGCCACCCC GAGTGCTCCC CTAATGAGGA CCGAGCAGCC 
CTCCAGTGCA TGTACTGCCA CGAGCTGTTT GTGGAGGAGA CGTCCCTCAT GAACCACATC 
GAGCAGGTAC ACGGGGGTGA GAAGAAGAAC TCTTGCAGCA TTTGCTCAGA GAGCTTCCTC 
ACGGTCGAGG AGCTGTATAG CCACATGGAC AGTCACCAGC AGCCAGAGTC CTGCAATCAC 
AGCAACAGCC CTTCCCTGGT CACTGTGGGT TACACCTCAG TGTCCAGCAC GACTCCAGAC 
TCGAACCTCT CAGTGGACAG CTCGACCATG GTAGAGGCTG CACCACCGAT TCCAAAGAGC 
CGCGGGAGGA AGCGAGCTGC TCAGCAGACC TCCGATATGA CTGGCCCCTC TAGTAAGCAG 
GCGAAAGTCA CCTACAGCTG TATTTACTGC AACAAGCAGT TATTTTCCAG TCTCGCGGTT 
CTGCAGATTC ACTTGAAAAC TATGCATTTA GATAAGCCTG AGCAGGCTCA TATCTGTCAG 
TATTGCTTGG AGGTCTTACC CTCACTCTAT AACCTAAATG AACATCTTAA ACAAGTGCAC 
GAAGCTCAGG ACCCCGGCCT GATTGTTTCG GCCATGCCTG CCATTGTTTA CCAGTGCAAC 
TTCTGTTCCG AAGTTGTCAA TGACCTCAAC ACCCTTCAGG AGCACATCCG ATGTTCTCAT 
GGGTTTGCCA ATCCCGCGGC CAAGGACAGC AATGCGTTCT TTTGTCCCCA TTGTTACATG 
GGGTTTCTCA CTGACTCTTC ACTTGAAGAG CATATAAGAC AGGTCCATTG TGACCTCAGT 
GGCTCCCGGT TTGGGTCTCC TGTGCTTGGG ACTCCAAAAG AACCGGTGGT TGAAGTCTAC 
TCCTGTTCCT ATTGTACAAA TTCGCCAATA TTCAACAGTG TTCTTAAACT GAATAAGCAT 
ATTAAAGAGA ATCATAAAAA CATTCCCTTG GCCCTGAATT ATATTCACAA TGGGAAGAAA 
TCCCGGGCCT TGAGCCCTTT GTCCCCTGTG GCTATAGAAC AAACAACTCT TAAGATGATG 
CAGACTGTGG GAGGCGGGCC TGCCCGCGCC TCTGGAGAGT ATATCTGTAA TCAGTGTGGT 
GCTAAGTACA CGTCCCTAGA CAGCTTTCAG ACTCACCTCA AAACCCATTT GGACACCGTG 
CTGCCAAAAC TGACCTGCCC TCAGTGTAAC AAAGAATTCC CCAACCAAGA GTCCTTGCTA 
AAGCACGTGA CCATCCACTT TATGATCACC TCAACCTACT ACATCTGTGA GAGCTGTGAC 
AAGCAGTTCA CCTCCGTGGA TGACCTCCAG AAGCACCTGC TGGACATGCA CACCTTCGTC 
TTCTTCCGCT GCACACTCTG CCAGGAAGTG TTCGACTCCA AGGTCTCTAT CCAGCTGCAC 
TTGGCCGTGA AGCACAGTAA CGAGAAGAAG GTGTACCGCT GCACGTCCTG CAACTGGGAC 
TTCCGCAACG AGACCGACCT GCAGCTGCAC GTGAAGCACA ACCACTTGGA GAACCAAGGC 
AAAGTGCACA AGTGCATCTT CTGCGGCGAG TCGTTTGGCA CCGAGGTGGA GCTGCAGTGC 
CACATCACCA CCCACAGCAA GAAGTACAAC TGCAGGTTCT GCAGCAAAGC CTTCCACGCC 
GTCATCCTGC TGGAGAAGCA CCTGCGGGAG AAACACTGTG TGTTTGAAAC CAAGACCCCC 
AACTGTGGCA CCAACGGGGC CTCGGAGCAA GTGCAGAAGG AGGAGGCTGA GCTGCAGACC 
TTGCTCACCA ACAGCCAGGA GTCCCATAAC AGCCATGATG GGAGTGAGGA GGACGTGGAC 
AGCTCTGAAC CCATGTATGG CTGTGACATC TGCGGGGCGG CCTACACCAT GGAGACGTTG 
CTGCAGAACC ACCAGCTCCG AGACCACAAC ATCAGGCCCG GAGAAAGCGC CATCGTGAAG 
AAGAAGGCGG AGCTCATTAA AGGGAATTAC AAGTGTAACG TTTGCTCGAG AACCTTCTTC 
TCCGAAAACG GGCTCCGTGA ACACATGCAG ACCCACTTAG GCCCGGTCAA ACACTACATG 
TGCCCTATCT GTGGAGAGCG CTTCCCTTCC CTGTTAACTC TCACAGAGCA CAAAGTCACA 
CACAGCAAGA GCCTGGATAC CGGAAACTGT CGTATTTGTA AGATGCCCCT GCAGAGCGAA 
GAGGAGTTTT TGGAGCACTG CCAGATGCAC CCTGACCTGA GGAATTCCCT GACTGGGTTT 
CGTTGTGTGG TGTGTATGCA GACTGTGACC TCCACCTTGG AACTCAAAAT CCACGGGACC 
TTCCACATGC AGAAGACGGG GAATGGGTCC TCCGTGCAGA CCACCGGGCG TGGCCAGCAC 
GTTCAGAAAC TGTATAAGTG CGCATCTTGC CTCAAAGAGT TCCGTTCCAA GCAAGATCTG 
GTGAAACTCG ACATCAACGG CCTGCCATAT GGTCTGTGTG CCGGCTGTGT GAATCTCAGT 
AAGAGCAGTA GCCCGGGCCT CAGTCTCCCT CCTGGTGCCA GCAGGCCAGG CTTGGGCCAG 
AATGAGAGTC TGAGCGCCAT GGAAGGAAAA GGCAAGGCGG GGGGACTGAA GACGCGCTGT 
TCAAGCTGCA ATGTCAAGTT TGAGTCTGAA AGCGAACTGC AGAACCACAT CCAGACGGTG 
CACCGGGAGC TTGTGCCAGA CGCCAACAGC ACACAGTTGA AAACGCCCCA AGTGTCACCC 
ATGCCCAGAA TCAGTCCCTC CCAGTCCGAT GAGAAGAAGA CCTACCAGTG TATCAAGTGT 
CAGATGGTTT TCTACAACGA GTGGGACATC CAGGTTCATG TGGCAAATCA CATGATTGAT 
GAAGGGCTGA ACCATGAGTG CAAACTCTGC AGCCAGACCT TTGACTCCCC CGCCAAACTT 
CAGTGTCACC TGATAGAGCA CAGCTTCGAA GGGATGGGTG GTACCTTCAA GTGCCCCGTC 
TGTTTCACAG TGTTTGTTCA AGCTAACAAG TTGCAACAGC ATATATTCTC TGCCCATGGA 
CAAGAAGACA AGATCTATGA CTGCACACAA TGCCCACAGA AGTTTTTCTT CCAAACAGAG 
CTACAGAATC ATACGATGAC TCAGCACAGC AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525883.1
CDS	2478..5753
Translation	MQVHERNKDGSQSGSRMEDWKMKDTQKCSQCEEGFDFPEDLQKHIAECHPECSPNEDRAALQCMYCHELFVEETSLMNHIEQVHGGEKKNSCSICSESFLTVEELYSHMDSHQQPESCNHSNSPSLVTVGYTSVSSTTPDSNLSVDSSTMVEAAPPIPKSRGRKRAAQQTSDMTGPSSKQAKVTYSCIYCNKQLFSSLAVLQIHLKTMHLDKPEQAHICQYCLEVLPSLYNLNEHLKQVHEAQDPGLIVSAMPAIVYQCNFCSEVVNDLNTLQEHIRCSHGFANPAAKDSNAFFCPHCYMGFLTDSSLEEHIRQVHCDLSGSRFGSPVLGTPKEPVVEVYSCSYCTNSPIFNSVLKLNKHIKENHKNIPLALNYIHNGKKSRALSPLSPVAIEQTTLKMMQTVGGGPARASGEYICNQCGAKYTSLDSFQTHLKTHLDTVLPKLTCPQCNKEFPNQESLLKHVTIHFMITSTYYICESCDKQFTSVDDLQKHLLDMHTFVFFRCTLCQEVFDSKVSIQLHLAVKHSNEKKVYRCTSCNWDFRNETDLQLHVKHNHLENQGKVHKCIFCGESFGTEVELQCHITTHSKKYNCRFCSKAFHAVILLEKHLREKHCVFETKTPNCGTNGASEQVQKEEAELQTLLTNSQESHNSHDGSEEDVDSSEPMYGCDICGAAYTMETLLQNHQLRDHNIRPGESAIVKKKAELIKGNYKCNVCSRTFFSENGLREHMQTHLGPVKHYMCPICGERFPSLLTLTEHKVTHSKSLDTGNCRICKMPLQSEEEFLEHCQMHPDLRNSLTGFRCVVCMQTVTSTLELKIHGTFHMQKTGNGSSVQTTGRGQHVQKLYKCASCLKEFRSKQDLVKLDINGLPYGLCAGCVNLSKSSSPGLSLPPGASRPGLGQNESLSAMEGKGKAGGLKTRCSSCNVKFESESELQNHIQTVHRELVPDANSTQLKTPQVSPMPRISPSQSDEKKTYQCIKCQMVFYNEWDIQVHVANHMIDEGLNHECKLCSQTFDSPAKLQCHLIEHSFEGMGGTFKCPVCFTVFVQANKLQQHIFSAHGQEDKIYDCTQCPQKFFFQTELQNHTMTQHSS

Target ORF information:

RefSeq Version	XM_006525820.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus zinc finger protein 521 (Zfp521), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525820.3

ORF Insert Sequence:

ATGCAGGTTC ATGAGCGGAA CAAGGACGGC TCCCAGTCTG GCTCCAGGAT GGAGGACTGG 
AAGATGAAGG ACACTCAGAA GTGCAGCCAG TGCGAGGAAG GCTTTGACTT CCCGGAAGAC 
CTCCAGAAGC ACATTGCAGA ATGCCACCCC GAGTGCTCCC CTAATGAGGA CCGAGCAGCC 
CTCCAGTGCA TGTACTGCCA CGAGCTGTTT GTGGAGGAGA CGTCCCTCAT GAACCACATC 
GAGCAGGTAC ACGGGGGTGA GAAGAAGAAC TCTTGCAGCA TTTGCTCAGA GAGCTTCCTC 
ACGGTCGAGG AGCTGTATAG CCACATGGAC AGTCACCAGC AGCCAGAGTC CTGCAATCAC 
AGCAACAGCC CTTCCCTGGT CACTGTGGGT TACACCTCAG TGTCCAGCAC GACTCCAGAC 
TCGAACCTCT CAGTGGACAG CTCGACCATG GTAGAGGCTG CACCACCGAT TCCAAAGAGC 
CGCGGGAGGA AGCGAGCTGC TCAGCAGACC TCCGATATGA CTGGCCCCTC TAGTAAGCAG 
GCGAAAGTCA CCTACAGCTG TATTTACTGC AACAAGCAGT TATTTTCCAG TCTCGCGGTT 
CTGCAGATTC ACTTGAAAAC TATGCATTTA GATAAGCCTG AGCAGGCTCA TATCTGTCAG 
TATTGCTTGG AGGTCTTACC CTCACTCTAT AACCTAAATG AACATCTTAA ACAAGTGCAC 
GAAGCTCAGG ACCCCGGCCT GATTGTTTCG GCCATGCCTG CCATTGTTTA CCAGTGCAAC 
TTCTGTTCCG AAGTTGTCAA TGACCTCAAC ACCCTTCAGG AGCACATCCG ATGTTCTCAT 
GGGTTTGCCA ATCCCGCGGC CAAGGACAGC AATGCGTTCT TTTGTCCCCA TTGTTACATG 
GGGTTTCTCA CTGACTCTTC ACTTGAAGAG CATATAAGAC AGGTCCATTG TGACCTCAGT 
GGCTCCCGGT TTGGGTCTCC TGTGCTTGGG ACTCCAAAAG AACCGGTGGT TGAAGTCTAC 
TCCTGTTCCT ATTGTACAAA TTCGCCAATA TTCAACAGTG TTCTTAAACT GAATAAGCAT 
ATTAAAGAGA ATCATAAAAA CATTCCCTTG GCCCTGAATT ATATTCACAA TGGGAAGAAA 
TCCCGGGCCT TGAGCCCTTT GTCCCCTGTG GCTATAGAAC AAACAACTCT TAAGATGATG 
CAGACTGTGG GAGGCGGGCC TGCCCGCGCC TCTGGAGAGT ATATCTGTAA TCAGTGTGGT 
GCTAAGTACA CGTCCCTAGA CAGCTTTCAG ACTCACCTCA AAACCCATTT GGACACCGTG 
CTGCCAAAAC TGACCTGCCC TCAGTGTAAC AAAGAATTCC CCAACCAAGA GTCCTTGCTA 
AAGCACGTGA CCATCCACTT TATGATCACC TCAACCTACT ACATCTGTGA GAGCTGTGAC 
AAGCAGTTCA CCTCCGTGGA TGACCTCCAG AAGCACCTGC TGGACATGCA CACCTTCGTC 
TTCTTCCGCT GCACACTCTG CCAGGAAGTG TTCGACTCCA AGGTCTCTAT CCAGCTGCAC 
TTGGCCGTGA AGCACAGTAA CGAGAAGAAG GTGTACCGCT GCACGTCCTG CAACTGGGAC 
TTCCGCAACG AGACCGACCT GCAGCTGCAC GTGAAGCACA ACCACTTGGA GAACCAAGGC 
AAAGTGCACA AGTGCATCTT CTGCGGCGAG TCGTTTGGCA CCGAGGTGGA GCTGCAGTGC 
CACATCACCA CCCACAGCAA GAAGTACAAC TGCAGGTTCT GCAGCAAAGC CTTCCACGCC 
GTCATCCTGC TGGAGAAGCA CCTGCGGGAG AAACACTGTG TGTTTGAAAC CAAGACCCCC 
AACTGTGGCA CCAACGGGGC CTCGGAGCAA GTGCAGAAGG AGGAGGCTGA GCTGCAGACC 
TTGCTCACCA ACAGCCAGGA GTCCCATAAC AGCCATGATG GGAGTGAGGA GGACGTGGAC 
AGCTCTGAAC CCATGTATGG CTGTGACATC TGCGGGGCGG CCTACACCAT GGAGACGTTG 
CTGCAGAACC ACCAGCTCCG AGACCACAAC ATCAGGCCCG GAGAAAGCGC CATCGTGAAG 
AAGAAGGCGG AGCTCATTAA AGGGAATTAC AAGTGTAACG TTTGCTCGAG AACCTTCTTC 
TCCGAAAACG GGCTCCGTGA ACACATGCAG ACCCACTTAG GCCCGGTCAA ACACTACATG 
TGCCCTATCT GTGGAGAGCG CTTCCCTTCC CTGTTAACTC TCACAGAGCA CAAAGTCACA 
CACAGCAAGA GCCTGGATAC CGGAAACTGT CGTATTTGTA AGATGCCCCT GCAGAGCGAA 
GAGGAGTTTT TGGAGCACTG CCAGATGCAC CCTGACCTGA GGAATTCCCT GACTGGGTTT 
CGTTGTGTGG TGTGTATGCA GACTGTGACC TCCACCTTGG AACTCAAAAT CCACGGGACC 
TTCCACATGC AGAAGACGGG GAATGGGTCC TCCGTGCAGA CCACCGGGCG TGGCCAGCAC 
GTTCAGAAAC TGTATAAGTG CGCATCTTGC CTCAAAGAGT TCCGTTCCAA GCAAGATCTG 
GTGAAACTCG ACATCAACGG CCTGCCATAT GGTCTGTGTG CCGGCTGTGT GAATCTCAGT 
AAGAGCAGTA GCCCGGGCCT CAGTCTCCCT CCTGGTGCCA GCAGGCCAGG CTTGGGCCAG 
AATGAGAGTC TGAGCGCCAT GGAAGGAAAA GGCAAGGCGG GGGGACTGAA GACGCGCTGT 
TCAAGCTGCA ATGTCAAGTT TGAGTCTGAA AGCGAACTGC AGAACCACAT CCAGACGGTG 
CACCGGGAGC TTGTGCCAGA CGCCAACAGC ACACAGTTGA AAACGCCCCA AGTGTCACCC 
ATGCCCAGAA TCAGTCCCTC CCAGTCCGAT GAGAAGAAGA CCTACCAGTG TATCAAGTGT 
CAGATGGTTT TCTACAACGA GTGGGACATC CAGGTTCATG TGGCAAATCA CATGATTGAT 
GAAGGGCTGA ACCATGAGTG CAAACTCTGC AGCCAGACCT TTGACTCCCC CGCCAAACTT 
CAGTGTCACC TGATAGAGCA CAGCTTCGAA GGGATGGGTG GTACCTTCAA GTGCCCCGTC 
TGTTTCACAG TGTTTGTTCA AGCTAACAAG TTGCAACAGC ATATATTCTC TGCCCATGGA 
CAAGAAGACA AGATCTATGA CTGCACACAA TGCCCACAGA AGTTTTTCTT CCAAACAGAG 
CTACAGAATC ATACGATGAC TCAGCACAGC AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Zfp521 ( XM_006525819.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006525882.1 Mus musculus zinc finger protein 521 (Zfp521), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu54502
Clone ID Related Accession (Same CDS sequence)	XM_006525820.4 , XM_006525820.3 , XM_006525819.3
Accession Version	XM_006525819.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	zinc finger protein 521 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006525819.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGTTC ATGAGCGGAA CAAGGACGGC TCCCAGTCTG GCTCCAGGAT GGAGGACTGG 
AAGATGAAGG ACACTCAGAA GTGCAGCCAG TGCGAGGAAG GCTTTGACTT CCCGGAAGAC 
CTCCAGAAGC ACATTGCAGA ATGCCACCCC GAGTGCTCCC CTAATGAGGA CCGAGCAGCC 
CTCCAGTGCA TGTACTGCCA CGAGCTGTTT GTGGAGGAGA CGTCCCTCAT GAACCACATC 
GAGCAGGTAC ACGGGGGTGA GAAGAAGAAC TCTTGCAGCA TTTGCTCAGA GAGCTTCCTC 
ACGGTCGAGG AGCTGTATAG CCACATGGAC AGTCACCAGC AGCCAGAGTC CTGCAATCAC 
AGCAACAGCC CTTCCCTGGT CACTGTGGGT TACACCTCAG TGTCCAGCAC GACTCCAGAC 
TCGAACCTCT CAGTGGACAG CTCGACCATG GTAGAGGCTG CACCACCGAT TCCAAAGAGC 
CGCGGGAGGA AGCGAGCTGC TCAGCAGACC TCCGATATGA CTGGCCCCTC TAGTAAGCAG 
GCGAAAGTCA CCTACAGCTG TATTTACTGC AACAAGCAGT TATTTTCCAG TCTCGCGGTT 
CTGCAGATTC ACTTGAAAAC TATGCATTTA GATAAGCCTG AGCAGGCTCA TATCTGTCAG 
TATTGCTTGG AGGTCTTACC CTCACTCTAT AACCTAAATG AACATCTTAA ACAAGTGCAC 
GAAGCTCAGG ACCCCGGCCT GATTGTTTCG GCCATGCCTG CCATTGTTTA CCAGTGCAAC 
TTCTGTTCCG AAGTTGTCAA TGACCTCAAC ACCCTTCAGG AGCACATCCG ATGTTCTCAT 
GGGTTTGCCA ATCCCGCGGC CAAGGACAGC AATGCGTTCT TTTGTCCCCA TTGTTACATG 
GGGTTTCTCA CTGACTCTTC ACTTGAAGAG CATATAAGAC AGGTCCATTG TGACCTCAGT 
GGCTCCCGGT TTGGGTCTCC TGTGCTTGGG ACTCCAAAAG AACCGGTGGT TGAAGTCTAC 
TCCTGTTCCT ATTGTACAAA TTCGCCAATA TTCAACAGTG TTCTTAAACT GAATAAGCAT 
ATTAAAGAGA ATCATAAAAA CATTCCCTTG GCCCTGAATT ATATTCACAA TGGGAAGAAA 
TCCCGGGCCT TGAGCCCTTT GTCCCCTGTG GCTATAGAAC AAACAACTCT TAAGATGATG 
CAGACTGTGG GAGGCGGGCC TGCCCGCGCC TCTGGAGAGT ATATCTGTAA TCAGTGTGGT 
GCTAAGTACA CGTCCCTAGA CAGCTTTCAG ACTCACCTCA AAACCCATTT GGACACCGTG 
CTGCCAAAAC TGACCTGCCC TCAGTGTAAC AAAGAATTCC CCAACCAAGA GTCCTTGCTA 
AAGCACGTGA CCATCCACTT TATGATCACC TCAACCTACT ACATCTGTGA GAGCTGTGAC 
AAGCAGTTCA CCTCCGTGGA TGACCTCCAG AAGCACCTGC TGGACATGCA CACCTTCGTC 
TTCTTCCGCT GCACACTCTG CCAGGAAGTG TTCGACTCCA AGGTCTCTAT CCAGCTGCAC 
TTGGCCGTGA AGCACAGTAA CGAGAAGAAG GTGTACCGCT GCACGTCCTG CAACTGGGAC 
TTCCGCAACG AGACCGACCT GCAGCTGCAC GTGAAGCACA ACCACTTGGA GAACCAAGGC 
AAAGTGCACA AGTGCATCTT CTGCGGCGAG TCGTTTGGCA CCGAGGTGGA GCTGCAGTGC 
CACATCACCA CCCACAGCAA GAAGTACAAC TGCAGGTTCT GCAGCAAAGC CTTCCACGCC 
GTCATCCTGC TGGAGAAGCA CCTGCGGGAG AAACACTGTG TGTTTGAAAC CAAGACCCCC 
AACTGTGGCA CCAACGGGGC CTCGGAGCAA GTGCAGAAGG AGGAGGCTGA GCTGCAGACC 
TTGCTCACCA ACAGCCAGGA GTCCCATAAC AGCCATGATG GGAGTGAGGA GGACGTGGAC 
AGCTCTGAAC CCATGTATGG CTGTGACATC TGCGGGGCGG CCTACACCAT GGAGACGTTG 
CTGCAGAACC ACCAGCTCCG AGACCACAAC ATCAGGCCCG GAGAAAGCGC CATCGTGAAG 
AAGAAGGCGG AGCTCATTAA AGGGAATTAC AAGTGTAACG TTTGCTCGAG AACCTTCTTC 
TCCGAAAACG GGCTCCGTGA ACACATGCAG ACCCACTTAG GCCCGGTCAA ACACTACATG 
TGCCCTATCT GTGGAGAGCG CTTCCCTTCC CTGTTAACTC TCACAGAGCA CAAAGTCACA 
CACAGCAAGA GCCTGGATAC CGGAAACTGT CGTATTTGTA AGATGCCCCT GCAGAGCGAA 
GAGGAGTTTT TGGAGCACTG CCAGATGCAC CCTGACCTGA GGAATTCCCT GACTGGGTTT 
CGTTGTGTGG TGTGTATGCA GACTGTGACC TCCACCTTGG AACTCAAAAT CCACGGGACC 
TTCCACATGC AGAAGACGGG GAATGGGTCC TCCGTGCAGA CCACCGGGCG TGGCCAGCAC 
GTTCAGAAAC TGTATAAGTG CGCATCTTGC CTCAAAGAGT TCCGTTCCAA GCAAGATCTG 
GTGAAACTCG ACATCAACGG CCTGCCATAT GGTCTGTGTG CCGGCTGTGT GAATCTCAGT 
AAGAGCAGTA GCCCGGGCCT CAGTCTCCCT CCTGGTGCCA GCAGGCCAGG CTTGGGCCAG 
AATGAGAGTC TGAGCGCCAT GGAAGGAAAA GGCAAGGCGG GGGGACTGAA GACGCGCTGT 
TCAAGCTGCA ATGTCAAGTT TGAGTCTGAA AGCGAACTGC AGAACCACAT CCAGACGGTG 
CACCGGGAGC TTGTGCCAGA CGCCAACAGC ACACAGTTGA AAACGCCCCA AGTGTCACCC 
ATGCCCAGAA TCAGTCCCTC CCAGTCCGAT GAGAAGAAGA CCTACCAGTG TATCAAGTGT 
CAGATGGTTT TCTACAACGA GTGGGACATC CAGGTTCATG TGGCAAATCA CATGATTGAT 
GAAGGGCTGA ACCATGAGTG CAAACTCTGC AGCCAGACCT TTGACTCCCC CGCCAAACTT 
CAGTGTCACC TGATAGAGCA CAGCTTCGAA GGGATGGGTG GTACCTTCAA GTGCCCCGTC 
TGTTTCACAG TGTTTGTTCA AGCTAACAAG TTGCAACAGC ATATATTCTC TGCCCATGGA 
CAAGAAGACA AGATCTATGA CTGCACACAA TGCCCACAGA AGTTTTTCTT CCAAACAGAG 
CTACAGAATC ATACGATGAC TCAGCACAGC AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006525882.1
CDS	870..4145
Translation	MQVHERNKDGSQSGSRMEDWKMKDTQKCSQCEEGFDFPEDLQKHIAECHPECSPNEDRAALQCMYCHELFVEETSLMNHIEQVHGGEKKNSCSICSESFLTVEELYSHMDSHQQPESCNHSNSPSLVTVGYTSVSSTTPDSNLSVDSSTMVEAAPPIPKSRGRKRAAQQTSDMTGPSSKQAKVTYSCIYCNKQLFSSLAVLQIHLKTMHLDKPEQAHICQYCLEVLPSLYNLNEHLKQVHEAQDPGLIVSAMPAIVYQCNFCSEVVNDLNTLQEHIRCSHGFANPAAKDSNAFFCPHCYMGFLTDSSLEEHIRQVHCDLSGSRFGSPVLGTPKEPVVEVYSCSYCTNSPIFNSVLKLNKHIKENHKNIPLALNYIHNGKKSRALSPLSPVAIEQTTLKMMQTVGGGPARASGEYICNQCGAKYTSLDSFQTHLKTHLDTVLPKLTCPQCNKEFPNQESLLKHVTIHFMITSTYYICESCDKQFTSVDDLQKHLLDMHTFVFFRCTLCQEVFDSKVSIQLHLAVKHSNEKKVYRCTSCNWDFRNETDLQLHVKHNHLENQGKVHKCIFCGESFGTEVELQCHITTHSKKYNCRFCSKAFHAVILLEKHLREKHCVFETKTPNCGTNGASEQVQKEEAELQTLLTNSQESHNSHDGSEEDVDSSEPMYGCDICGAAYTMETLLQNHQLRDHNIRPGESAIVKKKAELIKGNYKCNVCSRTFFSENGLREHMQTHLGPVKHYMCPICGERFPSLLTLTEHKVTHSKSLDTGNCRICKMPLQSEEEFLEHCQMHPDLRNSLTGFRCVVCMQTVTSTLELKIHGTFHMQKTGNGSSVQTTGRGQHVQKLYKCASCLKEFRSKQDLVKLDINGLPYGLCAGCVNLSKSSSPGLSLPPGASRPGLGQNESLSAMEGKGKAGGLKTRCSSCNVKFESESELQNHIQTVHRELVPDANSTQLKTPQVSPMPRISPSQSDEKKTYQCIKCQMVFYNEWDIQVHVANHMIDEGLNHECKLCSQTFDSPAKLQCHLIEHSFEGMGGTFKCPVCFTVFVQANKLQQHIFSAHGQEDKIYDCTQCPQKFFFQTELQNHTMTQHSS

Target ORF information:

RefSeq Version	XM_006525819.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus zinc finger protein 521 (Zfp521), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006525819.3

ORF Insert Sequence:

ATGCAGGTTC ATGAGCGGAA CAAGGACGGC TCCCAGTCTG GCTCCAGGAT GGAGGACTGG 
AAGATGAAGG ACACTCAGAA GTGCAGCCAG TGCGAGGAAG GCTTTGACTT CCCGGAAGAC 
CTCCAGAAGC ACATTGCAGA ATGCCACCCC GAGTGCTCCC CTAATGAGGA CCGAGCAGCC 
CTCCAGTGCA TGTACTGCCA CGAGCTGTTT GTGGAGGAGA CGTCCCTCAT GAACCACATC 
GAGCAGGTAC ACGGGGGTGA GAAGAAGAAC TCTTGCAGCA TTTGCTCAGA GAGCTTCCTC 
ACGGTCGAGG AGCTGTATAG CCACATGGAC AGTCACCAGC AGCCAGAGTC CTGCAATCAC 
AGCAACAGCC CTTCCCTGGT CACTGTGGGT TACACCTCAG TGTCCAGCAC GACTCCAGAC 
TCGAACCTCT CAGTGGACAG CTCGACCATG GTAGAGGCTG CACCACCGAT TCCAAAGAGC 
CGCGGGAGGA AGCGAGCTGC TCAGCAGACC TCCGATATGA CTGGCCCCTC TAGTAAGCAG 
GCGAAAGTCA CCTACAGCTG TATTTACTGC AACAAGCAGT TATTTTCCAG TCTCGCGGTT 
CTGCAGATTC ACTTGAAAAC TATGCATTTA GATAAGCCTG AGCAGGCTCA TATCTGTCAG 
TATTGCTTGG AGGTCTTACC CTCACTCTAT AACCTAAATG AACATCTTAA ACAAGTGCAC 
GAAGCTCAGG ACCCCGGCCT GATTGTTTCG GCCATGCCTG CCATTGTTTA CCAGTGCAAC 
TTCTGTTCCG AAGTTGTCAA TGACCTCAAC ACCCTTCAGG AGCACATCCG ATGTTCTCAT 
GGGTTTGCCA ATCCCGCGGC CAAGGACAGC AATGCGTTCT TTTGTCCCCA TTGTTACATG 
GGGTTTCTCA CTGACTCTTC ACTTGAAGAG CATATAAGAC AGGTCCATTG TGACCTCAGT 
GGCTCCCGGT TTGGGTCTCC TGTGCTTGGG ACTCCAAAAG AACCGGTGGT TGAAGTCTAC 
TCCTGTTCCT ATTGTACAAA TTCGCCAATA TTCAACAGTG TTCTTAAACT GAATAAGCAT 
ATTAAAGAGA ATCATAAAAA CATTCCCTTG GCCCTGAATT ATATTCACAA TGGGAAGAAA 
TCCCGGGCCT TGAGCCCTTT GTCCCCTGTG GCTATAGAAC AAACAACTCT TAAGATGATG 
CAGACTGTGG GAGGCGGGCC TGCCCGCGCC TCTGGAGAGT ATATCTGTAA TCAGTGTGGT 
GCTAAGTACA CGTCCCTAGA CAGCTTTCAG ACTCACCTCA AAACCCATTT GGACACCGTG 
CTGCCAAAAC TGACCTGCCC TCAGTGTAAC AAAGAATTCC CCAACCAAGA GTCCTTGCTA 
AAGCACGTGA CCATCCACTT TATGATCACC TCAACCTACT ACATCTGTGA GAGCTGTGAC 
AAGCAGTTCA CCTCCGTGGA TGACCTCCAG AAGCACCTGC TGGACATGCA CACCTTCGTC 
TTCTTCCGCT GCACACTCTG CCAGGAAGTG TTCGACTCCA AGGTCTCTAT CCAGCTGCAC 
TTGGCCGTGA AGCACAGTAA CGAGAAGAAG GTGTACCGCT GCACGTCCTG CAACTGGGAC 
TTCCGCAACG AGACCGACCT GCAGCTGCAC GTGAAGCACA ACCACTTGGA GAACCAAGGC 
AAAGTGCACA AGTGCATCTT CTGCGGCGAG TCGTTTGGCA CCGAGGTGGA GCTGCAGTGC 
CACATCACCA CCCACAGCAA GAAGTACAAC TGCAGGTTCT GCAGCAAAGC CTTCCACGCC 
GTCATCCTGC TGGAGAAGCA CCTGCGGGAG AAACACTGTG TGTTTGAAAC CAAGACCCCC 
AACTGTGGCA CCAACGGGGC CTCGGAGCAA GTGCAGAAGG AGGAGGCTGA GCTGCAGACC 
TTGCTCACCA ACAGCCAGGA GTCCCATAAC AGCCATGATG GGAGTGAGGA GGACGTGGAC 
AGCTCTGAAC CCATGTATGG CTGTGACATC TGCGGGGCGG CCTACACCAT GGAGACGTTG 
CTGCAGAACC ACCAGCTCCG AGACCACAAC ATCAGGCCCG GAGAAAGCGC CATCGTGAAG 
AAGAAGGCGG AGCTCATTAA AGGGAATTAC AAGTGTAACG TTTGCTCGAG AACCTTCTTC 
TCCGAAAACG GGCTCCGTGA ACACATGCAG ACCCACTTAG GCCCGGTCAA ACACTACATG 
TGCCCTATCT GTGGAGAGCG CTTCCCTTCC CTGTTAACTC TCACAGAGCA CAAAGTCACA 
CACAGCAAGA GCCTGGATAC CGGAAACTGT CGTATTTGTA AGATGCCCCT GCAGAGCGAA 
GAGGAGTTTT TGGAGCACTG CCAGATGCAC CCTGACCTGA GGAATTCCCT GACTGGGTTT 
CGTTGTGTGG TGTGTATGCA GACTGTGACC TCCACCTTGG AACTCAAAAT CCACGGGACC 
TTCCACATGC AGAAGACGGG GAATGGGTCC TCCGTGCAGA CCACCGGGCG TGGCCAGCAC 
GTTCAGAAAC TGTATAAGTG CGCATCTTGC CTCAAAGAGT TCCGTTCCAA GCAAGATCTG 
GTGAAACTCG ACATCAACGG CCTGCCATAT GGTCTGTGTG CCGGCTGTGT GAATCTCAGT 
AAGAGCAGTA GCCCGGGCCT CAGTCTCCCT CCTGGTGCCA GCAGGCCAGG CTTGGGCCAG 
AATGAGAGTC TGAGCGCCAT GGAAGGAAAA GGCAAGGCGG GGGGACTGAA GACGCGCTGT 
TCAAGCTGCA ATGTCAAGTT TGAGTCTGAA AGCGAACTGC AGAACCACAT CCAGACGGTG 
CACCGGGAGC TTGTGCCAGA CGCCAACAGC ACACAGTTGA AAACGCCCCA AGTGTCACCC 
ATGCCCAGAA TCAGTCCCTC CCAGTCCGAT GAGAAGAAGA CCTACCAGTG TATCAAGTGT 
CAGATGGTTT TCTACAACGA GTGGGACATC CAGGTTCATG TGGCAAATCA CATGATTGAT 
GAAGGGCTGA ACCATGAGTG CAAACTCTGC AGCCAGACCT TTGACTCCCC CGCCAAACTT 
CAGTGTCACC TGATAGAGCA CAGCTTCGAA GGGATGGGTG GTACCTTCAA GTGCCCCGTC 
TGTTTCACAG TGTTTGTTCA AGCTAACAAG TTGCAACAGC ATATATTCTC TGCCCATGGA 
CAAGAAGACA AGATCTATGA CTGCACACAA TGCCCACAGA AGTTTTTCTT CCAAACAGAG 
CTACAGAATC ATACGATGAC TCAGCACAGC AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Zfp521 ( XM_011246894.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011245196.1 Mus musculus zinc finger protein 521 (Zfp521), transcript variant X1, mRNA.

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CloneID	OMu73236
Clone ID Related Accession (Same CDS sequence)	XM_011246894.1 , NM_001360804.1
Accession Version	XM_011246894.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3933bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	zinc finger protein 521 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000084.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGCC GCAAGCAAGC GAAACCGAGA TCCCTCAAAG ACCCCAACTG TAAACTTGAA 
GACAAGATTG AAGATGGGGA GGCAGTAGAC TGCAAGAAGA GGCCAGAAGA CGGGGAGGAG 
TTGGAGGAGG ACGCTGTGCA CAGCTGTGAC AGCTGCCTCC AGGTGTTTGA GTCACTGAGC 
GACATCACAG AGCACAAGAT CCATCAGTGC CAGCTGACAG ATGGCGTTGA CGTTGAGGAC 
GACCCCAGCT GCTCTTGGCC AGCCTCCTCG CCTTCCAGTA AGGACCAGAC TTCCCCCAGC 
CATGGAGAAG GGTGTGATTT CGGAGAGGAA GAAGGTGGCC CTGGACTGCC TTACCCATGC 
CAGTTCTGTG ACAAGTCCTT TAGCCGCCTC AGCTACCTAA AGCACCATGA ACAGAGCCAC 
AGTGACAAGC TGCCCTTCAA GTGCACCTAC TGCAGTCGGC TGTTCAAACA CAAGCGGAGC 
CGTGACCGCC ACATAAAGCT CCACACTGGG GATAAGAAGT ATCACTGTAG CGAGTGTGAC 
GCAGCCTTCT CCCGGAGCGA TCATCTCAAG ATCCACTTAA AGACTCACAC CTCCAACAAG 
CCATATAAAT GTGCCGTCTG CCGCAGAGGG TTCCTCTCCT CTAGCTCCTT GCACGGACAT 
ATGCAGGTTC ATGAGCGGAA CAAGGACGGC TCCCAGTCTG GCTCCAGGAT GGAGGACTGG 
AAGATGAAGG ACACTCAGAA GTGCAGCCAG TGCGAGGAAG GCTTTGACTT CCCGGAAGAC 
CTCCAGAAGC ACATTGCAGA ATGCCACCCC GAGTGCTCCC CTAATGAGGA CCGAGCAGCC 
CTCCAGTGCA TGTACTGCCA CGAGCTGTTT GTGGAGGAGA CGTCCCTCAT GAACCACATC 
GAGCAGGTAC ACGGGGGTGA GAAGAAGAAC TCTTGCAGCA TTTGCTCAGA GAGCTTCCTC 
ACGGTCGAGG AGCTGTATAG CCACATGGAC AGTCACCAGC AGCCAGAGTC CTGCAATCAC 
AGCAACAGCC CTTCCCTGGT CACTGTGGGT TACACCTCAG TGTCCAGCAC GACTCCAGAC 
TCGAACCTCT CAGTGGACAG CTCGACCATG GTAGAGGCTG CACCACCGAT TCCAAAGAGC 
CGCGGGAGGA AGCGAGCTGC TCAGCAGACC TCCGATATGA CTGGCCCCTC TAGTAAGCAG 
GCGAAAGTCA CCTACAGCTG TATTTACTGC AACAAGCAGT TATTTTCCAG TCTCGCGGTT 
CTGCAGATTC ACTTGAAAAC TATGCATTTA GATAAGCCTG AGCAGGCTCA TATCTGTCAG 
TATTGCTTGG AGGTCTTACC CTCACTCTAT AACCTAAATG AACATCTTAA ACAAGTGCAC 
GAAGCTCAGG ACCCCGGCCT GATTGTTTCG GCCATGCCTG CCATTGTTTA CCAGTGCAAC 
TTCTGTTCCG AAGTTGTCAA TGACCTCAAC ACCCTTCAGG AGCACATCCG ATGTTCTCAT 
GGGTTTGCCA ATCCCGCGGC CAAGGACAGC AATGCGTTCT TTTGTCCCCA TTGTTACATG 
GGGTTTCTCA CTGACTCTTC ACTTGAAGAG CATATAAGAC AGGTCCATTG TGACCTCAGT 
GGCTCCCGGT TTGGGTCTCC TGTGCTTGGG ACTCCAAAAG AACCGGTGGT TGAAGTCTAC 
TCCTGTTCCT ATTGTACAAA TTCGCCAATA TTCAACAGTG TTCTTAAACT GAATAAGCAT 
ATTAAAGAGA ATCATAAAAA CATTCCCTTG GCCCTGAATT ATATTCACAA TGGGAAGAAA 
TCCCGGGCCT TGAGCCCTTT GTCCCCTGTG GCTATAGAAC AAACAACTCT TAAGATGATG 
CAGACTGTGG GAGGCGGGCC TGCCCGCGCC TCTGGAGAGT ATATCTGTAA TCAGTGTGGT 
GCTAAGTACA CGTCCCTAGA CAGCTTTCAG ACTCACCTCA AAACCCATTT GGACACCGTG 
CTGCCAAAAC TGACCTGCCC TCAGTGTAAC AAAGAATTCC CCAACCAAGA GTCCTTGCTA 
AAGCACGTGA CCATCCACTT TATGATCACC TCAACCTACT ACATCTGTGA GAGCTGTGAC 
AAGCAGTTCA CCTCCGTGGA TGACCTCCAG AAGCACCTGC TGGACATGCA CACCTTCGTC 
TTCTTCCGCT GCACACTCTG CCAGGAAGTG TTCGACTCCA AGGTCTCTAT CCAGCTGCAC 
TTGGCCGTGA AGCACAGTAA CGAGAAGAAG GTGTACCGCT GCACGTCCTG CAACTGGGAC 
TTCCGCAACG AGACCGACCT GCAGCTGCAC GTGAAGCACA ACCACTTGGA GAACCAAGGC 
AAAGTGCACA AGTGCATCTT CTGCGGCGAG TCGTTTGGCA CCGAGGTGGA GCTGCAGTGC 
CACATCACCA CCCACAGCAA GAAGTACAAC TGCAGGTTCT GCAGCAAAGC CTTCCACGCC 
GTCATCCTGC TGGAGAAGCA CCTGCGGGAG AAACACTGTG TGTTTGAAAC CAAGACCCCC 
AACTGTGGCA CCAACGGGGC CTCGGAGCAA GTGCAGAAGG AGGAGGCTGA GCTGCAGACC 
TTGCTCACCA ACAGCCAGGA GTCCCATAAC AGCCATGATG GGAGTGAGGA GGACGTGGAC 
AGCTCTGAAC CCATGTATGG CTGTGACATC TGCGGGGCGG CCTACACCAT GGAGACGTTG 
CTGCAGAACC ACCAGCTCCG AGACCACAAC ATCAGGCCCG GAGAAAGCGC CATCGTGAAG 
AAGAAGGCGG AGCTCATTAA AGGGAATTAC AAGTGTAACG TTTGCTCGAG AACCTTCTTC 
TCCGAAAACG GGCTCCGTGA ACACATGCAG ACCCACTTAG GCCCGGTCAA ACACTACATG 
TGCCCTATCT GTGGAGAGCG CTTCCCTTCC CTGTTAACTC TCACAGAGCA CAAAGTCACA 
CACAGCAAGA GCCTGGATAC CGGAAACTGT CGTATTTGTA AGATGCCCCT GCAGAGCGAA 
GAGGAGTTTT TGGAGCACTG CCAGATGCAC CCTGACCTGA GGAATTCCCT GACTGGGTTT 
CGTTGTGTGG TGTGTATGCA GACTGTGACC TCCACCTTGG AACTCAAAAT CCACGGGACC 
TTCCACATGC AGAAGACGGG GAATGGGTCC TCCGTGCAGA CCACCGGGCG TGGCCAGCAC 
GTTCAGAAAC TGTATAAGTG CGCATCTTGC CTCAAAGAGT TCCGTTCCAA GCAAGATCTG 
GTGAAACTCG ACATCAACGG CCTGCCATAT GGTCTGTGTG CCGGCTGTGT GAATCTCAGT 
AAGAGCAGTA GCCCGGGCCT CAGTCTCCCT CCTGGTGCCA GCAGGCCAGG CTTGGGCCAG 
AATGAGAGTC TGAGCGCCAT GGAAGGAAAA GGCAAGGCGG GGGGACTGAA GACGCGCTGT 
TCAAGCTGCA ATGTCAAGTT TGAGTCTGAA AGCGAACTGC AGAACCACAT CCAGACGGTG 
CACCGGGAGC TTGTGCCAGA CGCCAACAGC ACACAGTTGA AAACGCCCCA AGTGTCACCC 
ATGCCCAGAA TCAGTCCCTC CCAGTCCGAT GAGAAGACCT ACCAGTGTAT CAAGTGTCAG 
ATGGTTTTCT ACAACGAGTG GGACATCCAG GTTCATGTGG CAAATCACAT GATTGATGAA 
GGGCTGAACC ATGAGTGCAA ACTCTGCAGC CAGACCTTTG ACTCCCCCGC CAAACTTCAG 
TGTCACCTGA TAGAGCACAG CTTCGAAGGG ATGGGTGGTA CCTTCAAGTG CCCCGTCTGT 
TTCACAGTGT TTGTTCAAGC TAACAAGTTG CAACAGCATA TATTCTCTGC CCATGGACAA 
GAAGACAAGA TCTATGACTG CACACAATGC CCACAGAAGT TTTTCTTCCA AACAGAGCTA 
CAGAATCATA CGATGACTCA GCACAGCAGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011245196.1
CDS	289..4221
Translation	MSRRKQAKPRSLKDPNCKLEDKIEDGEAVDCKKRPEDGEELEEDAVHSCDSCLQVFESLSDITEHKIHQCQLTDGVDVEDDPSCSWPASSPSSKDQTSPSHGEGCDFGEEEGGPGLPYPCQFCDKSFSRLSYLKHHEQSHSDKLPFKCTYCSRLFKHKRSRDRHIKLHTGDKKYHCSECDAAFSRSDHLKIHLKTHTSNKPYKCAVCRRGFLSSSSLHGHMQVHERNKDGSQSGSRMEDWKMKDTQKCSQCEEGFDFPEDLQKHIAECHPECSPNEDRAALQCMYCHELFVEETSLMNHIEQVHGGEKKNSCSICSESFLTVEELYSHMDSHQQPESCNHSNSPSLVTVGYTSVSSTTPDSNLSVDSSTMVEAAPPIPKSRGRKRAAQQTSDMTGPSSKQAKVTYSCIYCNKQLFSSLAVLQIHLKTMHLDKPEQAHICQYCLEVLPSLYNLNEHLKQVHEAQDPGLIVSAMPAIVYQCNFCSEVVNDLNTLQEHIRCSHGFANPAAKDSNAFFCPHCYMGFLTDSSLEEHIRQVHCDLSGSRFGSPVLGTPKEPVVEVYSCSYCTNSPIFNSVLKLNKHIKENHKNIPLALNYIHNGKKSRALSPLSPVAIEQTTLKMMQTVGGGPARASGEYICNQCGAKYTSLDSFQTHLKTHLDTVLPKLTCPQCNKEFPNQESLLKHVTIHFMITSTYYICESCDKQFTSVDDLQKHLLDMHTFVFFRCTLCQEVFDSKVSIQLHLAVKHSNEKKVYRCTSCNWDFRNETDLQLHVKHNHLENQGKVHKCIFCGESFGTEVELQCHITTHSKKYNCRFCSKAFHAVILLEKHLREKHCVFETKTPNCGTNGASEQVQKEEAELQTLLTNSQESHNSHDGSEEDVDSSEPMYGCDICGAAYTMETLLQNHQLRDHNIRPGESAIVKKKAELIKGNYKCNVCSRTFFSENGLREHMQTHLGPVKHYMCPICGERFPSLLTLTEHKVTHSKSLDTGNCRICKMPLQSEEEFLEHCQMHPDLRNSLTGFRCVVCMQTVTSTLELKIHGTFHMQKTGNGSSVQTTGRGQHVQKLYKCASCLKEFRSKQDLVKLDINGLPYGLCAGCVNLSKSSSPGLSLPPGASRPGLGQNESLSAMEGKGKAGGLKTRCSSCNVKFESESELQNHIQTVHRELVPDANSTQLKTPQVSPMPRISPSQSDEKTYQCIKCQMVFYNEWDIQVHVANHMIDEGLNHECKLCSQTFDSPAKLQCHLIEHSFEGMGGTFKCPVCFTVFVQANKLQQHIFSAHGQEDKIYDCTQCPQKFFFQTELQNHTMTQHSS

Target ORF information:

RefSeq Version	XM_011246894.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus zinc finger protein 521 (Zfp521), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246894.1

ORF Insert Sequence:

ATGTCTCGCC GCAAGCAAGC GAAACCGAGA TCCCTCAAAG ACCCCAACTG TAAACTTGAA 
GACAAGATTG AAGATGGGGA GGCAGTAGAC TGCAAGAAGA GGCCAGAAGA CGGGGAGGAG 
TTGGAGGAGG ACGCTGTGCA CAGCTGTGAC AGCTGCCTCC AGGTGTTTGA GTCACTGAGC 
GACATCACAG AGCACAAGAT CCATCAGTGC CAGCTGACAG ATGGCGTTGA CGTTGAGGAC 
GACCCCAGCT GCTCTTGGCC AGCCTCCTCG CCTTCCAGTA AGGACCAGAC TTCCCCCAGC 
CATGGAGAAG GGTGTGATTT CGGAGAGGAA GAAGGTGGCC CTGGACTGCC TTACCCATGC 
CAGTTCTGTG ACAAGTCCTT TAGCCGCCTC AGCTACCTAA AGCACCATGA ACAGAGCCAC 
AGTGACAAGC TGCCCTTCAA GTGCACCTAC TGCAGTCGGC TGTTCAAACA CAAGCGGAGC 
CGTGACCGCC ACATAAAGCT CCACACTGGG GATAAGAAGT ATCACTGTAG CGAGTGTGAC 
GCAGCCTTCT CCCGGAGCGA TCATCTCAAG ATCCACTTAA AGACTCACAC CTCCAACAAG 
CCATATAAAT GTGCCGTCTG CCGCAGAGGG TTCCTCTCCT CTAGCTCCTT GCACGGACAT 
ATGCAGGTTC ATGAGCGGAA CAAGGACGGC TCCCAGTCTG GCTCCAGGAT GGAGGACTGG 
AAGATGAAGG ACACTCAGAA GTGCAGCCAG TGCGAGGAAG GCTTTGACTT CCCGGAAGAC 
CTCCAGAAGC ACATTGCAGA ATGCCACCCC GAGTGCTCCC CTAATGAGGA CCGAGCAGCC 
CTCCAGTGCA TGTACTGCCA CGAGCTGTTT GTGGAGGAGA CGTCCCTCAT GAACCACATC 
GAGCAGGTAC ACGGGGGTGA GAAGAAGAAC TCTTGCAGCA TTTGCTCAGA GAGCTTCCTC 
ACGGTCGAGG AGCTGTATAG CCACATGGAC AGTCACCAGC AGCCAGAGTC CTGCAATCAC 
AGCAACAGCC CTTCCCTGGT CACTGTGGGT TACACCTCAG TGTCCAGCAC GACTCCAGAC 
TCGAACCTCT CAGTGGACAG CTCGACCATG GTAGAGGCTG CACCACCGAT TCCAAAGAGC 
CGCGGGAGGA AGCGAGCTGC TCAGCAGACC TCCGATATGA CTGGCCCCTC TAGTAAGCAG 
GCGAAAGTCA CCTACAGCTG TATTTACTGC AACAAGCAGT TATTTTCCAG TCTCGCGGTT 
CTGCAGATTC ACTTGAAAAC TATGCATTTA GATAAGCCTG AGCAGGCTCA TATCTGTCAG 
TATTGCTTGG AGGTCTTACC CTCACTCTAT AACCTAAATG AACATCTTAA ACAAGTGCAC 
GAAGCTCAGG ACCCCGGCCT GATTGTTTCG GCCATGCCTG CCATTGTTTA CCAGTGCAAC 
TTCTGTTCCG AAGTTGTCAA TGACCTCAAC ACCCTTCAGG AGCACATCCG ATGTTCTCAT 
GGGTTTGCCA ATCCCGCGGC CAAGGACAGC AATGCGTTCT TTTGTCCCCA TTGTTACATG 
GGGTTTCTCA CTGACTCTTC ACTTGAAGAG CATATAAGAC AGGTCCATTG TGACCTCAGT 
GGCTCCCGGT TTGGGTCTCC TGTGCTTGGG ACTCCAAAAG AACCGGTGGT TGAAGTCTAC 
TCCTGTTCCT ATTGTACAAA TTCGCCAATA TTCAACAGTG TTCTTAAACT GAATAAGCAT 
ATTAAAGAGA ATCATAAAAA CATTCCCTTG GCCCTGAATT ATATTCACAA TGGGAAGAAA 
TCCCGGGCCT TGAGCCCTTT GTCCCCTGTG GCTATAGAAC AAACAACTCT TAAGATGATG 
CAGACTGTGG GAGGCGGGCC TGCCCGCGCC TCTGGAGAGT ATATCTGTAA TCAGTGTGGT 
GCTAAGTACA CGTCCCTAGA CAGCTTTCAG ACTCACCTCA AAACCCATTT GGACACCGTG 
CTGCCAAAAC TGACCTGCCC TCAGTGTAAC AAAGAATTCC CCAACCAAGA GTCCTTGCTA 
AAGCACGTGA CCATCCACTT TATGATCACC TCAACCTACT ACATCTGTGA GAGCTGTGAC 
AAGCAGTTCA CCTCCGTGGA TGACCTCCAG AAGCACCTGC TGGACATGCA CACCTTCGTC 
TTCTTCCGCT GCACACTCTG CCAGGAAGTG TTCGACTCCA AGGTCTCTAT CCAGCTGCAC 
TTGGCCGTGA AGCACAGTAA CGAGAAGAAG GTGTACCGCT GCACGTCCTG CAACTGGGAC 
TTCCGCAACG AGACCGACCT GCAGCTGCAC GTGAAGCACA ACCACTTGGA GAACCAAGGC 
AAAGTGCACA AGTGCATCTT CTGCGGCGAG TCGTTTGGCA CCGAGGTGGA GCTGCAGTGC 
CACATCACCA CCCACAGCAA GAAGTACAAC TGCAGGTTCT GCAGCAAAGC CTTCCACGCC 
GTCATCCTGC TGGAGAAGCA CCTGCGGGAG AAACACTGTG TGTTTGAAAC CAAGACCCCC 
AACTGTGGCA CCAACGGGGC CTCGGAGCAA GTGCAGAAGG AGGAGGCTGA GCTGCAGACC 
TTGCTCACCA ACAGCCAGGA GTCCCATAAC AGCCATGATG GGAGTGAGGA GGACGTGGAC 
AGCTCTGAAC CCATGTATGG CTGTGACATC TGCGGGGCGG CCTACACCAT GGAGACGTTG 
CTGCAGAACC ACCAGCTCCG AGACCACAAC ATCAGGCCCG GAGAAAGCGC CATCGTGAAG 
AAGAAGGCGG AGCTCATTAA AGGGAATTAC AAGTGTAACG TTTGCTCGAG AACCTTCTTC 
TCCGAAAACG GGCTCCGTGA ACACATGCAG ACCCACTTAG GCCCGGTCAA ACACTACATG 
TGCCCTATCT GTGGAGAGCG CTTCCCTTCC CTGTTAACTC TCACAGAGCA CAAAGTCACA 
CACAGCAAGA GCCTGGATAC CGGAAACTGT CGTATTTGTA AGATGCCCCT GCAGAGCGAA 
GAGGAGTTTT TGGAGCACTG CCAGATGCAC CCTGACCTGA GGAATTCCCT GACTGGGTTT 
CGTTGTGTGG TGTGTATGCA GACTGTGACC TCCACCTTGG AACTCAAAAT CCACGGGACC 
TTCCACATGC AGAAGACGGG GAATGGGTCC TCCGTGCAGA CCACCGGGCG TGGCCAGCAC 
GTTCAGAAAC TGTATAAGTG CGCATCTTGC CTCAAAGAGT TCCGTTCCAA GCAAGATCTG 
GTGAAACTCG ACATCAACGG CCTGCCATAT GGTCTGTGTG CCGGCTGTGT GAATCTCAGT 
AAGAGCAGTA GCCCGGGCCT CAGTCTCCCT CCTGGTGCCA GCAGGCCAGG CTTGGGCCAG 
AATGAGAGTC TGAGCGCCAT GGAAGGAAAA GGCAAGGCGG GGGGACTGAA GACGCGCTGT 
TCAAGCTGCA ATGTCAAGTT TGAGTCTGAA AGCGAACTGC AGAACCACAT CCAGACGGTG 
CACCGGGAGC TTGTGCCAGA CGCCAACAGC ACACAGTTGA AAACGCCCCA AGTGTCACCC 
ATGCCCAGAA TCAGTCCCTC CCAGTCCGAT GAGAAGACCT ACCAGTGTAT CAAGTGTCAG 
ATGGTTTTCT ACAACGAGTG GGACATCCAG GTTCATGTGG CAAATCACAT GATTGATGAA 
GGGCTGAACC ATGAGTGCAA ACTCTGCAGC CAGACCTTTG ACTCCCCCGC CAAACTTCAG 
TGTCACCTGA TAGAGCACAG CTTCGAAGGG ATGGGTGGTA CCTTCAAGTG CCCCGTCTGT 
TTCACAGTGT TTGTTCAAGC TAACAAGTTG CAACAGCATA TATTCTCTGC CCATGGACAA 
GAAGACAAGA TCTATGACTG CACACAATGC CCACAGAAGT TTTTCTTCCA AACAGAGCTA 
CAGAATCATA CGATGACTCA GCACAGCAGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Conserved expression of ultra-conserved noncoding RNA in mammalian nervous system.
Biochimica et biophysica acta1860(12)1159-1168(2017 Dec)
Zhou J,Wang R,Zhang J,Zhu L,Liu W,Lu S,Chen P,Li H,Yin B,Yuan J,Qiang B,Shu P,Peng X

ZFP521 regulates murine hematopoietic stem cell function and facilitates MLL-AF9 leukemogenesis in mouse and human cells.
Blood130(5)619-624(2017 08)
Garrison BS,Rybak AP,Beerman I,Heesters B,Mercier FE,Scadden DT,Bryder D,Baron R,Rossi DJ

Foxo1 is a downstream effector of Isl1 in direct pathway striatal projection neuron development within the embryonic mouse telencephalon.
Molecular and cellular neurosciences8044-51(2017 04)
Waclaw RR,Ehrman LA,Merchan-Sala P,Kohli V,Nardini D,Campbell K

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Zfp521 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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