Crb3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Crb3
Entrez Gene ID	224912
Full Name	crumbs family member 3
Synonyms	5730439B18,AU015179,AU041752
General protein information	Preferred Names crumbs family member 3 Names protein crumbs homolog 3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17\|17 D

mRNA and Protein(s)

mRNA	Protein	Name
XM_006524165.3	XP_006524228.1	protein crumbs homolog 3 isoform X1
XM_006524167.3	XP_006524230.1	protein crumbs homolog 3 isoform X2
NM_001359814.1	NP_001346743.1	protein crumbs homolog 3 isoform 2
NM_177638.4	NP_808306.1	protein crumbs homolog 3 isoform 1 precursor
NM_001347408.1	NP_001334337.1	protein crumbs homolog 3 isoform 2
NM_001359813.1	NP_001346742.1	protein crumbs homolog 3 isoform 1 precursor
NM_177638.5	NP_808306.1	protein crumbs homolog 3 isoform 1 precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Crb3	NP_808306.1
Rattus norvegicus (Norway rat)	Crb3	NP_001020832.1

Gene Ontology
Bibliography

Related articles in PubMed

EGFR signalling controls cellular fate and pancreatic organogenesis by regulating apicobasal polarity.
L?f-?hlin ZM, Nyeng P, Bechard ME, Hess K, Bankaitis E, Greiner TU, Ameri J, Wright CV, Semb H
Nature cell biology19(11)1313-1325(2017 Nov)

Asymmetric distribution of CRUMBS polarity complex proteins from compacted 8-cell to blastocyst stage during mouse preimplantation development.
Jiang X, An W, Yang X, Lin J, Ma S, Wang D, Tang S
Gene expression patterns : GEP2793-98(2017 Nov)

Apico-basal Polarity Determinants Encoded by
Hazime K, Malicki JJ
Genetics207(3)1041-1051(2017 Nov)

Llgl1 Connects Cell Polarity with Cell-Cell Adhesion in Embryonic Neural Stem Cells.
Jossin Y, Lee M, Klezovitch O, Kon E, Cossard A, Lien WH, Fernandez TE, Cooper JA, Vasioukhin V
Developmental cell41(5)481-495.e5(2017 06)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Crumbs3 sustains epithelial tissue morphogenesis and is essential to maintain apical membrane identity in kidney epithelial cells.
Title: Mouse Crumbs3 sustains epithelial tissue morphogenesis in vivo.

Crb3, a Crumbs isoform that determines epithelial apical domain identity, is required for airway differentiation by controlling the localization of the transcriptional regulator Yap.
Title: Crumbs3-Mediated Polarity Directs Airway Epithelial Cell Fate through the Hippo Pathway Effector Yap.

The presence of Crb3 in many organs and the regularity of Crb3 distribution in the process of mouse embryonic development indicate that Crb3 protein plays an important role in establishing and maintaining the polarity of mouse embryos.
Title: The expression and localization of Crb3 in developmental stages of the mice embryos and in different organs of 1-week-old female mice.

Data indicate that Crb3 (Crumbs3) is crucial for epithelial morphogenesis and plays a role in linking the apical membrane to the underlying ezrin-containing cytoskeleton.
Title: Crumbs3 is essential for proper epithelial development and viability.

the presence of CRB3 protein in the mouse retina
Title: Immunocytochemical evidence of the localization of the Crumbs homologue 3 protein (CRB3) in the developing and mature mouse retina.

The following Crb3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Crb3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu23263	NM_177638.5 Latest version!	Mus musculus crumbs family member 3 (Crb3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu23263	NM_001359813.1 Latest version!	Mus musculus crumbs family member 3 (Crb3), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu53511	NM_001359814.1 Latest version!	Mus musculus crumbs family member 3 (Crb3), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu53511	NM_001347408.1 Latest version!	Mus musculus crumbs family member 3 (Crb3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu23263	XM_006524167.3 Latest version!	Mus musculus crumbs family member 3 (Crb3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu53511	XM_006524165.3 Latest version!	Mus musculus crumbs family member 3 (Crb3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
View Old Accession Versions >>
OMu23263	NM_177638.4	Mus musculus crumbs family member 3 (Crb3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Crb3 ( NM_177638.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_808306.1 Mus musculus crumbs family member 3 (Crb3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu23263
Clone ID Related Accession (Same CDS sequence)	XM_006524167.3 , NM_177638.4 , NM_001359813.1 , NM_177638.5
Accession Version	NM_177638.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-14
Organism	Mus musculus(house mouse)
Product	protein crumbs homolog 3 isoform 1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CT571247.13. On Nov 17, 2006 this sequence version replaced NM_177638.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC024462.1, BI659084.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCGACCC CAGGCCTGGG GGTGCTCTTG GCGTTTGGTT TGCCGATGCT GCCCTCCGGC 
TGGAGCCTAA CAGCACCGGA CCCTTTCACA AATAGCACAA CTCAACCCCC AGGTGATGAG 
TCCAACGGGG GCCTGTCTTC AGGGGCTATT GTAGCCATCA CTGTGGTCTT TTCCATTCTG 
GGAGTCCTCC TTATAGCAGT GGGACTGTTT CTGCTCATGC GAAAACTTCG GGAAAAGCGG 
CAGACAGAGG GCACCTACCG GCCCAGCAGT GAGGAGCAGG TGGGTGCCCG TGCTCCACCA 
CCCCCCAACC TCAAGCTGCC CCCGGAGGAG CGGCTCATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_808306.1
CDS	197..538
Translation	MATPGLGVLLAFGLPMLPSGWSLTAPDPFTNSTTQPPGDESNGGLSSGAIVAITVVFSILGVLLIAVGLFLLMRKLREKRQTEGTYRPSSEEQVGARAPPPPNLKLPPEERLI

Target ORF information:

RefSeq Version	NM_177638.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus crumbs family member 3 (Crb3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177638.4

ORF Insert Sequence:

ATGGCGACCC CAGGCCTGGG GGTGCTCTTG GCGTTTGGTT TGCCGATGCT GCCCTCCGGC 
TGGAGCCTAA CAGCACCGGA CCCTTTCACA AATAGCACAA CTCAACCCCC AGGTGATGAG 
TCCAACGGGG GCCTGTCTTC AGGGGCTATT GTAGCCATCA CTGTGGTCTT TTCCATTCTG 
GGAGTCCTCC TTATAGCAGT GGGACTGTTT CTGCTCATGC GAAAACTTCG GGAAAAGCGG 
CAGACAGAGG GCACCTACCG GCCCAGCAGT GAGGAGCAGG TGGGTGCCCG TGCTCCACCA 
CCCCCCAACC TCAAGCTGCC CCCGGAGGAG CGGCTCATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Crb3 ( NM_001359813.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001346742.1 Mus musculus crumbs family member 3 (Crb3), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu23263
Clone ID Related Accession (Same CDS sequence)	XM_006524167.3 , NM_177638.4 , NM_001359813.1 , NM_177638.5
Accession Version	NM_001359813.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	protein crumbs homolog 3 isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK077528.1, AK167279.1 and BP769883.1. On Jan 13, 2018 this sequence version replaced XM_006524167.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660811.93540.1, AK077528.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGACCC CAGGCCTGGG GGTGCTCTTG GCGTTTGGTT TGCCGATGCT GCCCTCCGGC 
TGGAGCCTAA CAGCACCGGA CCCTTTCACA AATAGCACAA CTCAACCCCC AGGTGATGAG 
TCCAACGGGG GCCTGTCTTC AGGGGCTATT GTAGCCATCA CTGTGGTCTT TTCCATTCTG 
GGAGTCCTCC TTATAGCAGT GGGACTGTTT CTGCTCATGC GAAAACTTCG GGAAAAGCGG 
CAGACAGAGG GCACCTACCG GCCCAGCAGT GAGGAGCAGG TGGGTGCCCG TGCTCCACCA 
CCCCCCAACC TCAAGCTGCC CCCGGAGGAG CGGCTCATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001346742.1
CDS	128..469
Translation	MATPGLGVLLAFGLPMLPSGWSLTAPDPFTNSTTQPPGDESNGGLSSGAIVAITVVFSILGVLLIAVGLFLLMRKLREKRQTEGTYRPSSEEQVGARAPPPPNLKLPPEERLI

Target ORF information:

RefSeq Version	NM_001359813.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus crumbs family member 3 (Crb3), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001359813.1

ORF Insert Sequence:

ATGGCGACCC CAGGCCTGGG GGTGCTCTTG GCGTTTGGTT TGCCGATGCT GCCCTCCGGC 
TGGAGCCTAA CAGCACCGGA CCCTTTCACA AATAGCACAA CTCAACCCCC AGGTGATGAG 
TCCAACGGGG GCCTGTCTTC AGGGGCTATT GTAGCCATCA CTGTGGTCTT TTCCATTCTG 
GGAGTCCTCC TTATAGCAGT GGGACTGTTT CTGCTCATGC GAAAACTTCG GGAAAAGCGG 
CAGACAGAGG GCACCTACCG GCCCAGCAGT GAGGAGCAGG TGGGTGCCCG TGCTCCACCA 
CCCCCCAACC TCAAGCTGCC CCCGGAGGAG CGGCTCATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Crb3 ( NM_177638.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_808306.1 Mus musculus crumbs family member 3 (Crb3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu23263
Clone ID Related Accession (Same CDS sequence)	XM_006524167.3 , NM_177638.4 , NM_001359813.1 , NM_177638.5
Accession Version	NM_177638.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	protein crumbs homolog 3 isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CT571247.13. On Jan 13, 2018 this sequence version replaced NM_177638.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC024462.1, BI659084.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGACCC CAGGCCTGGG GGTGCTCTTG GCGTTTGGTT TGCCGATGCT GCCCTCCGGC 
TGGAGCCTAA CAGCACCGGA CCCTTTCACA AATAGCACAA CTCAACCCCC AGGTGATGAG 
TCCAACGGGG GCCTGTCTTC AGGGGCTATT GTAGCCATCA CTGTGGTCTT TTCCATTCTG 
GGAGTCCTCC TTATAGCAGT GGGACTGTTT CTGCTCATGC GAAAACTTCG GGAAAAGCGG 
CAGACAGAGG GCACCTACCG GCCCAGCAGT GAGGAGCAGG TGGGTGCCCG TGCTCCACCA 
CCCCCCAACC TCAAGCTGCC CCCGGAGGAG CGGCTCATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_808306.1
CDS	243..584
Translation	MATPGLGVLLAFGLPMLPSGWSLTAPDPFTNSTTQPPGDESNGGLSSGAIVAITVVFSILGVLLIAVGLFLLMRKLREKRQTEGTYRPSSEEQVGARAPPPPNLKLPPEERLI

Target ORF information:

RefSeq Version	NM_177638.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus crumbs family member 3 (Crb3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177638.5

ORF Insert Sequence:

ATGGCGACCC CAGGCCTGGG GGTGCTCTTG GCGTTTGGTT TGCCGATGCT GCCCTCCGGC 
TGGAGCCTAA CAGCACCGGA CCCTTTCACA AATAGCACAA CTCAACCCCC AGGTGATGAG 
TCCAACGGGG GCCTGTCTTC AGGGGCTATT GTAGCCATCA CTGTGGTCTT TTCCATTCTG 
GGAGTCCTCC TTATAGCAGT GGGACTGTTT CTGCTCATGC GAAAACTTCG GGAAAAGCGG 
CAGACAGAGG GCACCTACCG GCCCAGCAGT GAGGAGCAGG TGGGTGCCCG TGCTCCACCA 
CCCCCCAACC TCAAGCTGCC CCCGGAGGAG CGGCTCATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Crb3 ( NM_001359814.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001346743.1 Mus musculus crumbs family member 3 (Crb3), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OMu53511
Clone ID Related Accession (Same CDS sequence)	XM_006524165.3 , NM_001359814.1 , NM_001347408.1
Accession Version	NM_001359814.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Mus musculus(house mouse)
Product	protein crumbs homolog 3 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CT571247.13. On Jan 13, 2018 this sequence version replaced XM_006524165.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660813.453444.1, SRR1660811.298452.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGACCC CAGGCCTGGG GGTGCTCTTG GCGTTTGGTT TGCCGATGCT GCCCTCCGGC 
TGGAGCCTAA CAGCACCGGA CCCTTTCACA AATAGCACAA CTCAACCCCC AGGTGATGAG 
TCCAACGGGG GCCTGTCTTC AGGGGCTATT GTAGCCATCA CTGTGGTCTT TTCCATTCTG 
GGAGTCCTCC TTATAGCAGT GGGACTGTTT CTGCTCATGC GAAAACTTCG GGAAAAGCGG 
CAGACAGAGG GCACCTACCG GCCCAGCAGT GAGGAGCAGT TTTCCCACGC AGCAGCTGAG 
GCCCGGGCCC CCCAGGACTC CAAGGAGCCA GTGCGGGGCT GCCTGCCCAT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001346743.1
CDS	128..481
Translation	MATPGLGVLLAFGLPMLPSGWSLTAPDPFTNSTTQPPGDESNGGLSSGAIVAITVVFSILGVLLIAVGLFLLMRKLREKRQTEGTYRPSSEEQFSHAAAEARAPQDSKEPVRGCLPI

Target ORF information:

RefSeq Version	NM_001359814.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus crumbs family member 3 (Crb3), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001359814.1

ORF Insert Sequence:

ATGGCGACCC CAGGCCTGGG GGTGCTCTTG GCGTTTGGTT TGCCGATGCT GCCCTCCGGC 
TGGAGCCTAA CAGCACCGGA CCCTTTCACA AATAGCACAA CTCAACCCCC AGGTGATGAG 
TCCAACGGGG GCCTGTCTTC AGGGGCTATT GTAGCCATCA CTGTGGTCTT TTCCATTCTG 
GGAGTCCTCC TTATAGCAGT GGGACTGTTT CTGCTCATGC GAAAACTTCG GGAAAAGCGG 
CAGACAGAGG GCACCTACCG GCCCAGCAGT GAGGAGCAGT TTTCCCACGC AGCAGCTGAG 
GCCCGGGCCC CCCAGGACTC CAAGGAGCCA GTGCGGGGCT GCCTGCCCAT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Crb3 ( NM_001347408.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001334337.1 Mus musculus crumbs family member 3 (Crb3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu53511
Clone ID Related Accession (Same CDS sequence)	XM_006524165.3 , NM_001359814.1 , NM_001347408.1
Accession Version	NM_001347408.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	protein crumbs homolog 3 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CT571247.13 and BP769883.1. On Nov 26, 2016 this sequence version replaced XM_006524164.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CF616302.1, CK030526.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGACCC CAGGCCTGGG GGTGCTCTTG GCGTTTGGTT TGCCGATGCT GCCCTCCGGC 
TGGAGCCTAA CAGCACCGGA CCCTTTCACA AATAGCACAA CTCAACCCCC AGGTGATGAG 
TCCAACGGGG GCCTGTCTTC AGGGGCTATT GTAGCCATCA CTGTGGTCTT TTCCATTCTG 
GGAGTCCTCC TTATAGCAGT GGGACTGTTT CTGCTCATGC GAAAACTTCG GGAAAAGCGG 
CAGACAGAGG GCACCTACCG GCCCAGCAGT GAGGAGCAGT TTTCCCACGC AGCAGCTGAG 
GCCCGGGCCC CCCAGGACTC CAAGGAGCCA GTGCGGGGCT GCCTGCCCAT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001334337.1
CDS	243..596
Translation	MATPGLGVLLAFGLPMLPSGWSLTAPDPFTNSTTQPPGDESNGGLSSGAIVAITVVFSILGVLLIAVGLFLLMRKLREKRQTEGTYRPSSEEQFSHAAAEARAPQDSKEPVRGCLPI

Target ORF information:

RefSeq Version	NM_001347408.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus crumbs family member 3 (Crb3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001347408.1

ORF Insert Sequence:

ATGGCGACCC CAGGCCTGGG GGTGCTCTTG GCGTTTGGTT TGCCGATGCT GCCCTCCGGC 
TGGAGCCTAA CAGCACCGGA CCCTTTCACA AATAGCACAA CTCAACCCCC AGGTGATGAG 
TCCAACGGGG GCCTGTCTTC AGGGGCTATT GTAGCCATCA CTGTGGTCTT TTCCATTCTG 
GGAGTCCTCC TTATAGCAGT GGGACTGTTT CTGCTCATGC GAAAACTTCG GGAAAAGCGG 
CAGACAGAGG GCACCTACCG GCCCAGCAGT GAGGAGCAGT TTTCCCACGC AGCAGCTGAG 
GCCCGGGCCC CCCAGGACTC CAAGGAGCCA GTGCGGGGCT GCCTGCCCAT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Crb3 ( XM_006524167.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524230.1 Mus musculus crumbs family member 3 (Crb3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu23263
Clone ID Related Accession (Same CDS sequence)	XM_006524167.3 , NM_177638.4 , NM_001359813.1 , NM_177638.5
Accession Version	XM_006524167.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein crumbs homolog 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006524167.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACCC CAGGCCTGGG GGTGCTCTTG GCGTTTGGTT TGCCGATGCT GCCCTCCGGC 
TGGAGCCTAA CAGCACCGGA CCCTTTCACA AATAGCACAA CTCAACCCCC AGGTGATGAG 
TCCAACGGGG GCCTGTCTTC AGGGGCTATT GTAGCCATCA CTGTGGTCTT TTCCATTCTG 
GGAGTCCTCC TTATAGCAGT GGGACTGTTT CTGCTCATGC GAAAACTTCG GGAAAAGCGG 
CAGACAGAGG GCACCTACCG GCCCAGCAGT GAGGAGCAGG TGGGTGCCCG TGCTCCACCA 
CCCCCCAACC TCAAGCTGCC CCCGGAGGAG CGGCTCATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524230.1
CDS	702..1043
Translation	MATPGLGVLLAFGLPMLPSGWSLTAPDPFTNSTTQPPGDESNGGLSSGAIVAITVVFSILGVLLIAVGLFLLMRKLREKRQTEGTYRPSSEEQVGARAPPPPNLKLPPEERLI

Target ORF information:

RefSeq Version	XM_006524167.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus crumbs family member 3 (Crb3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524167.3

ORF Insert Sequence:

ATGGCGACCC CAGGCCTGGG GGTGCTCTTG GCGTTTGGTT TGCCGATGCT GCCCTCCGGC 
TGGAGCCTAA CAGCACCGGA CCCTTTCACA AATAGCACAA CTCAACCCCC AGGTGATGAG 
TCCAACGGGG GCCTGTCTTC AGGGGCTATT GTAGCCATCA CTGTGGTCTT TTCCATTCTG 
GGAGTCCTCC TTATAGCAGT GGGACTGTTT CTGCTCATGC GAAAACTTCG GGAAAAGCGG 
CAGACAGAGG GCACCTACCG GCCCAGCAGT GAGGAGCAGG TGGGTGCCCG TGCTCCACCA 
CCCCCCAACC TCAAGCTGCC CCCGGAGGAG CGGCTCATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Crb3 ( XM_006524165.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524228.1 Mus musculus crumbs family member 3 (Crb3), transcript variant X2, mRNA.

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CloneID	OMu53511
Clone ID Related Accession (Same CDS sequence)	XM_006524165.3 , NM_001359814.1 , NM_001347408.1
Accession Version	XM_006524165.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein crumbs homolog 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006524165.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACCC CAGGCCTGGG GGTGCTCTTG GCGTTTGGTT TGCCGATGCT GCCCTCCGGC 
TGGAGCCTAA CAGCACCGGA CCCTTTCACA AATAGCACAA CTCAACCCCC AGGTGATGAG 
TCCAACGGGG GCCTGTCTTC AGGGGCTATT GTAGCCATCA CTGTGGTCTT TTCCATTCTG 
GGAGTCCTCC TTATAGCAGT GGGACTGTTT CTGCTCATGC GAAAACTTCG GGAAAAGCGG 
CAGACAGAGG GCACCTACCG GCCCAGCAGT GAGGAGCAGT TTTCCCACGC AGCAGCTGAG 
GCCCGGGCCC CCCAGGACTC CAAGGAGCCA GTGCGGGGCT GCCTGCCCAT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524228.1
CDS	702..1055
Translation	MATPGLGVLLAFGLPMLPSGWSLTAPDPFTNSTTQPPGDESNGGLSSGAIVAITVVFSILGVLLIAVGLFLLMRKLREKRQTEGTYRPSSEEQFSHAAAEARAPQDSKEPVRGCLPI

Target ORF information:

RefSeq Version	XM_006524165.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus crumbs family member 3 (Crb3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524165.3

ORF Insert Sequence:

ATGGCGACCC CAGGCCTGGG GGTGCTCTTG GCGTTTGGTT TGCCGATGCT GCCCTCCGGC 
TGGAGCCTAA CAGCACCGGA CCCTTTCACA AATAGCACAA CTCAACCCCC AGGTGATGAG 
TCCAACGGGG GCCTGTCTTC AGGGGCTATT GTAGCCATCA CTGTGGTCTT TTCCATTCTG 
GGAGTCCTCC TTATAGCAGT GGGACTGTTT CTGCTCATGC GAAAACTTCG GGAAAAGCGG 
CAGACAGAGG GCACCTACCG GCCCAGCAGT GAGGAGCAGT TTTCCCACGC AGCAGCTGAG 
GCCCGGGCCC CCCAGGACTC CAAGGAGCCA GTGCGGGGCT GCCTGCCCAT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

EGFR signalling controls cellular fate and pancreatic organogenesis by regulating apicobasal polarity.
Nature cell biology19(11)1313-1325(2017 Nov)
L?f-?hlin ZM,Nyeng P,Bechard ME,Hess K,Bankaitis E,Greiner TU,Ameri J,Wright CV,Semb H

Asymmetric distribution of CRUMBS polarity complex proteins from compacted 8-cell to blastocyst stage during mouse preimplantation development.
Gene expression patterns : GEP2793-98(2017 Nov)
Jiang X,An W,Yang X,Lin J,Ma S,Wang D,Tang S

Apico-basal Polarity Determinants Encoded by
Genetics207(3)1041-1051(2017 Nov)
Hazime K,Malicki JJ

Llgl1 Connects Cell Polarity with Cell-Cell Adhesion in Embryonic Neural Stem Cells.
Developmental cell41(5)481-495.e5(2017 06)
Jossin Y,Lee M,Klezovitch O,Kon E,Cossard A,Lien WH,Fernandez TE,Cooper JA,Vasioukhin V

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Crb3 cDNA ORF clone, Mus musculus(house mouse)

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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