Tfb1m cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tfb1m
Entrez Gene ID	224481
Full Name	transcription factor B1, mitochondrial
Synonyms	AI429207
General protein information	Preferred Names transcription factor B1, mitochondrial Names dimethyladenosine transferase 1, mitochondrial S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase 1 mitochondrial 12S rRNA dimethylase 1 mitochondrial transcription factor B1 mtTFB1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17\|17 A1

mRNA and Protein(s)

mRNA	Protein	Name
NM_146074.1	NP_666186.1	dimethyladenosine transferase 1, mitochondrial
XM_006523220.3	XP_006523283.1	dimethyladenosine transferase 1, mitochondrial isoform X1
NM_146074.2	NP_666186.1	dimethyladenosine transferase 1, mitochondrial
NM_146074.3	NP_666186.1	dimethyladenosine transferase 1, mitochondrial

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	TFB1M	XP_533459.2
Homo sapiens (human)	TFB1M	NP_057104.2
	TFB1M	XP_001094657.1
Bos taurus (cattle)	TFB1M	NP_001108082.1
Rattus norvegicus (Norway rat)	Tfb1m	NP_852139.2
Drosophila melanogaster (fruit fly)	mtTFB1	NP_648499.1
Pan troglodytes (chimpanzee)	TFB1M	XP_001143300.1
Mus musculus (house mouse)	Tfb1m	NP_666186.1
Caenorhabditis elegans (roundworm)	T03F1.7	NP_491242.2
Xenopus tropicalis (tropical clawed frog)	tfb1m	NP_001016494.1
Gallus gallus (chicken)	TFB1M	XP_004935701.1
Danio rerio (zebrafish)	tfb1m	NP_001070237.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012129	XP_320403.3

Gene Ontology
Bibliography

Related articles in PubMed

The Chromatin Remodeling Complex Chd4/NuRD Controls Striated Muscle Identity and Metabolic Homeostasis.
G?mez-Del Arco P, Perdiguero E, Yunes-Leites PS, Ac?n-P?rez R, Zeini M, Garcia-Gomez A, Sreenivasan K, Jim?nez-Alc?zar M, Segal?s J, L?pez-Maderuelo D, Orn?s B, Jim?nez-Borreguero LJ, D'Amato G, Enshell-Seijffers D, Morgan B, Georgopoulos K, Islam AB, Braun T, de la Pompa JL, Kim J, Enriquez JA, Ballestar E, Mu?oz-C?noves P, Redondo JM
Cell metabolism23(5)881-92(2016 May)

Overexpression of the mitochondrial methyltransferase TFB1M in the mouse does not impact mitoribosomal methylation status or hearing.
Lee S, Rose S, Metodiev MD, Becker L, Vernaleken A, Klopstock T, Gailus-Durner V, Fuchs H, Hrab? De Angelis M, Douthwaite S, Larsson NG
Human molecular genetics24(25)7286-94(2015 Dec)

Loss of TFB1M results in mitochondrial dysfunction that leads to impaired insulin secretion and diabetes.
Sharoyko VV, Abels M, Sun J, Nicholas LM, Mollet IG, Stamenkovic JA, G?hring I, Malmgren S, Storm P, Fadista J, Sp?gel P, Metodiev MD, Larsson NG, Eliasson L, Wierup N, Mulder H
Human molecular genetics23(21)5733-49(2014 Nov)

Methylation of 12S rRNA is necessary for in vivo stability of the small subunit of the mammalian mitochondrial ribosome.
Metodiev MD, Lesko N, Park CB, C?mara Y, Shi Y, Wibom R, Hultenby K, Gustafsson CM, Larsson NG
Cell metabolism9(4)386-97(2009 Apr)

Molecular control of mitochondrial function in preimplantation mouse embryos.
Thundathil J, Filion F, Smith LC
Molecular reproduction and development71(4)405-13(2005 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Loss of TFB1M results in mitochondrial dysfunction that leads to impaired insulin secretion and diabetes.
Title: Loss of TFB1M results in mitochondrial dysfunction that leads to impaired insulin secretion and diabetes.

Metazoans have two TFBM genes, suggesting that a duplication of the gene has permitted a more flexible regulation of mtDNA.
Title: Characterization of the mouse genes for mitochondrial transcription factors B1 and B2.

The following Tfb1m gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tfb1m cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu52842	XM_006523220.3 Latest version!	Mus musculus transcription factor B1, mitochondrial (Tfb1m), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu14465	NM_146074.3 Latest version!	Mus musculus transcription factor B1, mitochondrial (Tfb1m), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
View Old Accession Versions >>
OMu14465	NM_146074.1	Mus musculus transcription factor B1, mitochondrial (Tfb1m), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OMu14465	NM_146074.2	Mus musculus transcription factor B1, mitochondrial (Tfb1m), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tfb1m ( XM_006523220.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006523283.1 Mus musculus transcription factor B1, mitochondrial (Tfb1m), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu52842
Clone ID Related Accession (Same CDS sequence)	XM_006523220.3
Accession Version	XM_006523220.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	dimethyladenosine transferase 1, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006523220.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCCT CGGGGAAGCT CGGCACTTTC CGCCTTCCCC CGTTGCCCAC CATTCGGGAG 
ATCATTAAGT TGTTCGGACT GCGAGCGGTG AAACAGCTTT CCCAGAATTT CCTCCTGGAC 
TTGAGGCTGA CAGACAAGAT TGTAAGGAAA GCTGGCAGTC TAGCAGATGT TTATGTTTAT 
GAAGTGGGTC CTGGGCCCGG GGGAATCACA AGATCCATCC TGAATGCCAA CGTTGCTGAG 
CTTCTGGTGG TTGAAAAGGA CACTCGCTTT ATCCCAGGGT TACAGATGCT CTCTGATGCA 
GCACCTGGAA AACTCCGAAT TGTTCATGGA GATGTGCTGA CATACAAGAT AGAAAAGGCT 
TTTCCAGGCA ACATTAGAAG ACAGTGGGAA GATGATCCTC CAAATGTACA CATTATTGGA 
AATCTACCTT TTAGTGTTTC AACTCCACTG ATTATCAAGT GGCTTGAAAA TATTTCTCTT 
AAAGATGGCC CTTTCGTTTA TGGCAGAACT AAGATGACGT TAACGTTTCA AAAGGAAGTG 
GCTGAGAGAC TTGTAGCCAC TACAGGAAGC AAACAGCACA GTCGCCTTTC CATTATGGCC 
CAGTACCTCT GCAATGTTGA ACACCTCTTT ACAATCCCAG GAAAAGCTTT TGTTCCCAAA 
CCAAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006523283.1
CDS	65..733
Translation	MAASGKLGTFRLPPLPTIREIIKLFGLRAVKQLSQNFLLDLRLTDKIVRKAGSLADVYVYEVGPGPGGITRSILNANVAELLVVEKDTRFIPGLQMLSDAAPGKLRIVHGDVLTYKIEKAFPGNIRRQWEDDPPNVHIIGNLPFSVSTPLIIKWLENISLKDGPFVYGRTKMTLTFQKEVAERLVATTGSKQHSRLSIMAQYLCNVEHLFTIPGKAFVPKPK

Target ORF information:

RefSeq Version	XM_006523220.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus transcription factor B1, mitochondrial (Tfb1m), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006523220.3

ORF Insert Sequence:

ATGGCTGCCT CGGGGAAGCT CGGCACTTTC CGCCTTCCCC CGTTGCCCAC CATTCGGGAG 
ATCATTAAGT TGTTCGGACT GCGAGCGGTG AAACAGCTTT CCCAGAATTT CCTCCTGGAC 
TTGAGGCTGA CAGACAAGAT TGTAAGGAAA GCTGGCAGTC TAGCAGATGT TTATGTTTAT 
GAAGTGGGTC CTGGGCCCGG GGGAATCACA AGATCCATCC TGAATGCCAA CGTTGCTGAG 
CTTCTGGTGG TTGAAAAGGA CACTCGCTTT ATCCCAGGGT TACAGATGCT CTCTGATGCA 
GCACCTGGAA AACTCCGAAT TGTTCATGGA GATGTGCTGA CATACAAGAT AGAAAAGGCT 
TTTCCAGGCA ACATTAGAAG ACAGTGGGAA GATGATCCTC CAAATGTACA CATTATTGGA 
AATCTACCTT TTAGTGTTTC AACTCCACTG ATTATCAAGT GGCTTGAAAA TATTTCTCTT 
AAAGATGGCC CTTTCGTTTA TGGCAGAACT AAGATGACGT TAACGTTTCA AAAGGAAGTG 
GCTGAGAGAC TTGTAGCCAC TACAGGAAGC AAACAGCACA GTCGCCTTTC CATTATGGCC 
CAGTACCTCT GCAATGTTGA ACACCTCTTT ACAATCCCAG GAAAAGCTTT TGTTCCCAAA 
CCAAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tfb1m ( NM_146074.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_666186.1 Mus musculus transcription factor B1, mitochondrial (Tfb1m), mRNA.

Price & Availability↑

CloneID	OMu14465
Clone ID Related Accession (Same CDS sequence)	NM_146074.1 , NM_146074.2 , NM_146074.3
Accession Version	NM_146074.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1038bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Mus musculus(house mouse)
Product	dimethyladenosine transferase 1, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC032930.1. ##Evidence-Data-START## Transcript exon combination :: BC032930.1, AF508971.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164143 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGGCTGCCT CGGGGAAGCT CGGCACTTTC CGCCTTCCCC CGTTGCCCAC CATTCGGGAG 
ATCATTAAGT TGTTCGGACT GCGAGCGGTG AAACAGCTTT CCCAGAATTT CCTCCTGGAC 
TTGAGGCTGA CAGACAAGAT TGTAAGGAAA GCTGGCAGTC TAGCAGATGT TTATGTTTAT 
GAAGTGGGTC CTGGGCCCGG GGGAATCACA AGATCCATCC TGAATGCCAA CGTTGCTGAG 
CTTCTGGTGG TTGAAAAGGA CACTCGCTTT ATCCCAGGGT TACAGATGCT CTCTGATGCA 
GCACCTGGAA AACTCCGAAT TGTTCATGGA GATGTGCTGA CATACAAGAT AGAAAAGGCT 
TTTCCAGGCA ACATTAGAAG ACAGTGGGAA GATGATCCTC CAAATGTACA CATTATTGGA 
AATCTACCTT TTAGTGTTTC AACTCCACTG ATTATCAAGT GGCTTGAAAA TATTTCTCTT 
AAAGATGGCC CTTTCGTTTA TGGCAGAACT AAGATGACGT TAACGTTTCA AAAGGAAGTG 
GCTGAGAGAC TTGTAGCCAC TACAGGAAGC AAACAGCACA GTCGCCTTTC CATTATGGCC 
CAGTACCTCT GCAATGTTGA ACACCTCTTT ACAATCCCAG GAAAAGCTTT TGTTCCCAAA 
CCAAAAGTTG ATGTAGGAGT GGTGCACCTT ACGCCCTTGA TAGAGCCCAA GATCAAGCAG 
CCATTCAAGC TGGTAGAAAA AGTTGTCCAG AATGCGTTTC AGTTTCGAAG GAAATATTGC 
CACCGAGGGC TTGGAATGTT ATTCCCTGAA GCACAACGCC TCGAAAGCAC TGGAAGGCTG 
TTACAGTTGG CAGACATTGA TCCAACTCTC CGGCCCACCC ACCTCTCGCT CATGCATTTT 
AAGAGTTTGT GTGATGTATA CAGAAAAATG TGTGATGAAG ACCCACAACT CTTTACTTAT 
AACTTCAGAG AGGAGCTCAA GCAAAAGAAA AGTAAAGGCC AAGAAAAAGA TGGTGACCCA 
GAAAGCTGCG GGTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_666186.1
CDS	1..1038
Translation	MAASGKLGTFRLPPLPTIREIIKLFGLRAVKQLSQNFLLDLRLTDKIVRKAGSLADVYVYEVGPGPGGITRSILNANVAELLVVEKDTRFIPGLQMLSDAAPGKLRIVHGDVLTYKIEKAFPGNIRRQWEDDPPNVHIIGNLPFSVSTPLIIKWLENISLKDGPFVYGRTKMTLTFQKEVAERLVATTGSKQHSRLSIMAQYLCNVEHLFTIPGKAFVPKPKVDVGVVHLTPLIEPKIKQPFKLVEKVVQNAFQFRRKYCHRGLGMLFPEAQRLESTGRLLQLADIDPTLRPTHLSLMHFKSLCDVYRKMCDEDPQLFTYNFREELKQKKSKGQEKDGDPESCGF

Target ORF information:

RefSeq Version	NM_146074.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus transcription factor B1, mitochondrial (Tfb1m), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_146074.1

ORF Insert Sequence:

ATGGCTGCCT CGGGGAAGCT CGGCACTTTC CGCCTTCCCC CGTTGCCCAC CATTCGGGAG 
ATCATTAAGT TGTTCGGACT GCGAGCGGTG AAACAGCTTT CCCAGAATTT CCTCCTGGAC 
TTGAGGCTGA CAGACAAGAT TGTAAGGAAA GCTGGCAGTC TAGCAGATGT TTATGTTTAT 
GAAGTGGGTC CTGGGCCCGG GGGAATCACA AGATCCATCC TGAATGCCAA CGTTGCTGAG 
CTTCTGGTGG TTGAAAAGGA CACTCGCTTT ATCCCAGGGT TACAGATGCT CTCTGATGCA 
GCACCTGGAA AACTCCGAAT TGTTCATGGA GATGTGCTGA CATACAAGAT AGAAAAGGCT 
TTTCCAGGCA ACATTAGAAG ACAGTGGGAA GATGATCCTC CAAATGTACA CATTATTGGA 
AATCTACCTT TTAGTGTTTC AACTCCACTG ATTATCAAGT GGCTTGAAAA TATTTCTCTT 
AAAGATGGCC CTTTCGTTTA TGGCAGAACT AAGATGACGT TAACGTTTCA AAAGGAAGTG 
GCTGAGAGAC TTGTAGCCAC TACAGGAAGC AAACAGCACA GTCGCCTTTC CATTATGGCC 
CAGTACCTCT GCAATGTTGA ACACCTCTTT ACAATCCCAG GAAAAGCTTT TGTTCCCAAA 
CCAAAAGTTG ATGTAGGAGT GGTGCACCTT ACGCCCTTGA TAGAGCCCAA GATCAAGCAG 
CCATTCAAGC TGGTAGAAAA AGTTGTCCAG AATGCGTTTC AGTTTCGAAG GAAATATTGC 
CACCGAGGGC TTGGAATGTT ATTCCCTGAA GCACAACGCC TCGAAAGCAC TGGAAGGCTG 
TTACAGTTGG CAGACATTGA TCCAACTCTC CGGCCCACCC ACCTCTCGCT CATGCATTTT 
AAGAGTTTGT GTGATGTATA CAGAAAAATG TGTGATGAAG ACCCACAACT CTTTACTTAT 
AACTTCAGAG AGGAGCTCAA GCAAAAGAAA AGTAAAGGCC AAGAAAAAGA TGGTGACCCA 
GAAAGCTGCG GGTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tfb1m ( NM_146074.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_666186.1 Mus musculus transcription factor B1, mitochondrial (Tfb1m), mRNA.

Price & Availability↑

CloneID	OMu14465
Clone ID Related Accession (Same CDS sequence)	NM_146074.1 , NM_146074.2 , NM_146074.3
Accession Version	NM_146074.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1038bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-17
Organism	Mus musculus(house mouse)
Product	dimethyladenosine transferase 1, mitochondrial
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC098838.3, BC032930.1 and AW456865.1. On Jul 18, 2018 this sequence version replaced NM_146074.1. ##Evidence-Data-START## Transcript exon combination :: BC032930.1, AF508971.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164143 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTGCCT CGGGGAAGCT CGGCACTTTC CGCCTTCCCC CGTTGCCCAC CATTCGGGAG 
ATCATTAAGT TGTTCGGACT GCGAGCGGTG AAACAGCTTT CCCAGAATTT CCTCCTGGAC 
TTGAGGCTGA CAGACAAGAT TGTAAGGAAA GCTGGCAGTC TAGCAGATGT TTATGTTTAT 
GAAGTGGGTC CTGGGCCCGG GGGAATCACA AGATCCATCC TGAATGCCAA CGTTGCTGAG 
CTTCTGGTGG TTGAAAAGGA CACTCGCTTT ATCCCAGGGT TACAGATGCT CTCTGATGCA 
GCACCTGGAA AACTCCGAAT TGTTCATGGA GATGTGCTGA CATACAAGAT AGAAAAGGCT 
TTTCCAGGCA ACATTAGAAG ACAGTGGGAA GATGATCCTC CAAATGTACA CATTATTGGA 
AATCTACCTT TTAGTGTTTC AACTCCACTG ATTATCAAGT GGCTTGAAAA TATTTCTCTT 
AAAGATGGCC CTTTCGTTTA TGGCAGAACT AAGATGACGT TAACGTTTCA AAAGGAAGTG 
GCTGAGAGAC TTGTAGCCAC TACAGGAAGC AAACAGCACA GTCGCCTTTC CATTATGGCC 
CAGTACCTCT GCAATGTTGA ACACCTCTTT ACAATCCCAG GAAAAGCTTT TGTTCCCAAA 
CCAAAAGTTG ATGTAGGAGT GGTGCACCTT ACGCCCTTGA TAGAGCCCAA GATCAAGCAG 
CCATTCAAGC TGGTAGAAAA AGTTGTCCAG AATGCGTTTC AGTTTCGAAG GAAATATTGC 
CACCGAGGGC TTGGAATGTT ATTCCCTGAA GCACAACGCC TCGAAAGCAC TGGAAGGCTG 
TTACAGTTGG CAGACATTGA TCCAACTCTC CGGCCCACCC ACCTCTCGCT CATGCATTTT 
AAGAGTTTGT GTGATGTATA CAGAAAAATG TGTGATGAAG ACCCACAACT CTTTACTTAT 
AACTTCAGAG AGGAGCTCAA GCAAAAGAAA AGTAAAGGCC AAGAAAAAGA TGGTGACCCA 
GAAAGCTGCG GGTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_666186.1
CDS	43..1080
Translation	MAASGKLGTFRLPPLPTIREIIKLFGLRAVKQLSQNFLLDLRLTDKIVRKAGSLADVYVYEVGPGPGGITRSILNANVAELLVVEKDTRFIPGLQMLSDAAPGKLRIVHGDVLTYKIEKAFPGNIRRQWEDDPPNVHIIGNLPFSVSTPLIIKWLENISLKDGPFVYGRTKMTLTFQKEVAERLVATTGSKQHSRLSIMAQYLCNVEHLFTIPGKAFVPKPKVDVGVVHLTPLIEPKIKQPFKLVEKVVQNAFQFRRKYCHRGLGMLFPEAQRLESTGRLLQLADIDPTLRPTHLSLMHFKSLCDVYRKMCDEDPQLFTYNFREELKQKKSKGQEKDGDPESCGF

Target ORF information:

RefSeq Version	NM_146074.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus transcription factor B1, mitochondrial (Tfb1m), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_146074.2

ORF Insert Sequence:

ATGGCTGCCT CGGGGAAGCT CGGCACTTTC CGCCTTCCCC CGTTGCCCAC CATTCGGGAG 
ATCATTAAGT TGTTCGGACT GCGAGCGGTG AAACAGCTTT CCCAGAATTT CCTCCTGGAC 
TTGAGGCTGA CAGACAAGAT TGTAAGGAAA GCTGGCAGTC TAGCAGATGT TTATGTTTAT 
GAAGTGGGTC CTGGGCCCGG GGGAATCACA AGATCCATCC TGAATGCCAA CGTTGCTGAG 
CTTCTGGTGG TTGAAAAGGA CACTCGCTTT ATCCCAGGGT TACAGATGCT CTCTGATGCA 
GCACCTGGAA AACTCCGAAT TGTTCATGGA GATGTGCTGA CATACAAGAT AGAAAAGGCT 
TTTCCAGGCA ACATTAGAAG ACAGTGGGAA GATGATCCTC CAAATGTACA CATTATTGGA 
AATCTACCTT TTAGTGTTTC AACTCCACTG ATTATCAAGT GGCTTGAAAA TATTTCTCTT 
AAAGATGGCC CTTTCGTTTA TGGCAGAACT AAGATGACGT TAACGTTTCA AAAGGAAGTG 
GCTGAGAGAC TTGTAGCCAC TACAGGAAGC AAACAGCACA GTCGCCTTTC CATTATGGCC 
CAGTACCTCT GCAATGTTGA ACACCTCTTT ACAATCCCAG GAAAAGCTTT TGTTCCCAAA 
CCAAAAGTTG ATGTAGGAGT GGTGCACCTT ACGCCCTTGA TAGAGCCCAA GATCAAGCAG 
CCATTCAAGC TGGTAGAAAA AGTTGTCCAG AATGCGTTTC AGTTTCGAAG GAAATATTGC 
CACCGAGGGC TTGGAATGTT ATTCCCTGAA GCACAACGCC TCGAAAGCAC TGGAAGGCTG 
TTACAGTTGG CAGACATTGA TCCAACTCTC CGGCCCACCC ACCTCTCGCT CATGCATTTT 
AAGAGTTTGT GTGATGTATA CAGAAAAATG TGTGATGAAG ACCCACAACT CTTTACTTAT 
AACTTCAGAG AGGAGCTCAA GCAAAAGAAA AGTAAAGGCC AAGAAAAAGA TGGTGACCCA 
GAAAGCTGCG GGTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tfb1m ( NM_146074.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_666186.1 Mus musculus transcription factor B1, mitochondrial (Tfb1m), mRNA.

Price & Availability↑

CloneID	OMu14465
Clone ID Related Accession (Same CDS sequence)	NM_146074.1 , NM_146074.2 , NM_146074.3
Accession Version	NM_146074.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1038bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Mus musculus(house mouse)
Product	dimethyladenosine transferase 1, mitochondrial
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC098838.3 and AC122350.4. On Jun 24, 2019 this sequence version replaced NM_146074.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR3224858.12612.1, SRR5189679.113590.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164143 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTGCCT CGGGGAAGCT CGGCACTTTC CGCCTTCCCC CGTTGCCCAC CATTCGGGAG 
ATCATTAAGT TGTTCGGACT GCGAGCGGTG AAACAGCTTT CCCAGAATTT CCTCCTGGAC 
TTGAGGCTGA CAGACAAGAT TGTAAGGAAA GCTGGCAGTC TAGCAGATGT TTATGTTTAT 
GAAGTGGGTC CTGGGCCCGG GGGAATCACA AGATCCATCC TGAATGCCAA CGTTGCTGAG 
CTTCTGGTGG TTGAAAAGGA CACTCGCTTT ATCCCAGGGT TACAGATGCT CTCTGATGCA 
GCACCTGGAA AACTCCGAAT TGTTCATGGA GATGTGCTGA CATACAAGAT AGAAAAGGCT 
TTTCCAGGCA ACATTAGAAG ACAGTGGGAA GATGATCCTC CAAATGTACA CATTATTGGA 
AATCTACCTT TTAGTGTTTC AACTCCACTG ATTATCAAGT GGCTTGAAAA TATTTCTCTT 
AAAGATGGCC CTTTCGTTTA TGGCAGAACT AAGATGACGT TAACGTTTCA AAAGGAAGTG 
GCTGAGAGAC TTGTAGCCAC TACAGGAAGC AAACAGCACA GTCGCCTTTC CATTATGGCC 
CAGTACCTCT GCAATGTTGA ACACCTCTTT ACAATCCCAG GAAAAGCTTT TGTTCCCAAA 
CCAAAAGTTG ATGTAGGAGT GGTGCACCTT ACGCCCTTGA TAGAGCCCAA GATCAAGCAG 
CCATTCAAGC TGGTAGAAAA AGTTGTCCAG AATGCGTTTC AGTTTCGAAG GAAATATTGC 
CACCGAGGGC TTGGAATGTT ATTCCCTGAA GCACAACGCC TCGAAAGCAC TGGAAGGCTG 
TTACAGTTGG CAGACATTGA TCCAACTCTC CGGCCCACCC ACCTCTCGCT CATGCATTTT 
AAGAGTTTGT GTGATGTATA CAGAAAAATG TGTGATGAAG ACCCACAACT CTTTACTTAT 
AACTTCAGAG AGGAGCTCAA GCAAAAGAAA AGTAAAGGCC AAGAAAAAGA TGGTGACCCA 
GAAAGCTGCG GGTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_666186.1
CDS	43..1080
Translation	MAASGKLGTFRLPPLPTIREIIKLFGLRAVKQLSQNFLLDLRLTDKIVRKAGSLADVYVYEVGPGPGGITRSILNANVAELLVVEKDTRFIPGLQMLSDAAPGKLRIVHGDVLTYKIEKAFPGNIRRQWEDDPPNVHIIGNLPFSVSTPLIIKWLENISLKDGPFVYGRTKMTLTFQKEVAERLVATTGSKQHSRLSIMAQYLCNVEHLFTIPGKAFVPKPKVDVGVVHLTPLIEPKIKQPFKLVEKVVQNAFQFRRKYCHRGLGMLFPEAQRLESTGRLLQLADIDPTLRPTHLSLMHFKSLCDVYRKMCDEDPQLFTYNFREELKQKKSKGQEKDGDPESCGF

Target ORF information:

RefSeq Version	NM_146074.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus transcription factor B1, mitochondrial (Tfb1m), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_146074.3

ORF Insert Sequence:

ATGGCTGCCT CGGGGAAGCT CGGCACTTTC CGCCTTCCCC CGTTGCCCAC CATTCGGGAG 
ATCATTAAGT TGTTCGGACT GCGAGCGGTG AAACAGCTTT CCCAGAATTT CCTCCTGGAC 
TTGAGGCTGA CAGACAAGAT TGTAAGGAAA GCTGGCAGTC TAGCAGATGT TTATGTTTAT 
GAAGTGGGTC CTGGGCCCGG GGGAATCACA AGATCCATCC TGAATGCCAA CGTTGCTGAG 
CTTCTGGTGG TTGAAAAGGA CACTCGCTTT ATCCCAGGGT TACAGATGCT CTCTGATGCA 
GCACCTGGAA AACTCCGAAT TGTTCATGGA GATGTGCTGA CATACAAGAT AGAAAAGGCT 
TTTCCAGGCA ACATTAGAAG ACAGTGGGAA GATGATCCTC CAAATGTACA CATTATTGGA 
AATCTACCTT TTAGTGTTTC AACTCCACTG ATTATCAAGT GGCTTGAAAA TATTTCTCTT 
AAAGATGGCC CTTTCGTTTA TGGCAGAACT AAGATGACGT TAACGTTTCA AAAGGAAGTG 
GCTGAGAGAC TTGTAGCCAC TACAGGAAGC AAACAGCACA GTCGCCTTTC CATTATGGCC 
CAGTACCTCT GCAATGTTGA ACACCTCTTT ACAATCCCAG GAAAAGCTTT TGTTCCCAAA 
CCAAAAGTTG ATGTAGGAGT GGTGCACCTT ACGCCCTTGA TAGAGCCCAA GATCAAGCAG 
CCATTCAAGC TGGTAGAAAA AGTTGTCCAG AATGCGTTTC AGTTTCGAAG GAAATATTGC 
CACCGAGGGC TTGGAATGTT ATTCCCTGAA GCACAACGCC TCGAAAGCAC TGGAAGGCTG 
TTACAGTTGG CAGACATTGA TCCAACTCTC CGGCCCACCC ACCTCTCGCT CATGCATTTT 
AAGAGTTTGT GTGATGTATA CAGAAAAATG TGTGATGAAG ACCCACAACT CTTTACTTAT 
AACTTCAGAG AGGAGCTCAA GCAAAAGAAA AGTAAAGGCC AAGAAAAAGA TGGTGACCCA 
GAAAGCTGCG GGTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Chromatin Remodeling Complex Chd4/NuRD Controls Striated Muscle Identity and Metabolic Homeostasis.
Cell metabolism23(5)881-92(2016 May)
G?mez-Del Arco P,Perdiguero E,Yunes-Leites PS,Ac?n-P?rez R,Zeini M,Garcia-Gomez A,Sreenivasan K,Jim?nez-Alc?zar M,Segal?s J,L?pez-Maderuelo D,Orn?s B,Jim?nez-Borreguero LJ,D'Amato G,Enshell-Seijffers D,Morgan B,Georgopoulos K,Islam AB,Braun T,de la Pompa JL,Kim J,Enriquez JA,Ballestar E,Mu?oz-C?noves P,Redondo JM

Overexpression of the mitochondrial methyltransferase TFB1M in the mouse does not impact mitoribosomal methylation status or hearing.
Human molecular genetics24(25)7286-94(2015 Dec)
Lee S,Rose S,Metodiev MD,Becker L,Vernaleken A,Klopstock T,Gailus-Durner V,Fuchs H,Hrab? De Angelis M,Douthwaite S,Larsson NG

Loss of TFB1M results in mitochondrial dysfunction that leads to impaired insulin secretion and diabetes.
Human molecular genetics23(21)5733-49(2014 Nov)
Sharoyko VV,Abels M,Sun J,Nicholas LM,Mollet IG,Stamenkovic JA,G?hring I,Malmgren S,Storm P,Fadista J,Sp?gel P,Metodiev MD,Larsson NG,Eliasson L,Wierup N,Mulder H

Methylation of 12S rRNA is necessary for in vivo stability of the small subunit of the mammalian mitochondrial ribosome.
Cell metabolism9(4)386-97(2009 Apr)
Metodiev MD,Lesko N,Park CB,C?mara Y,Shi Y,Wibom R,Hultenby K,Gustafsson CM,Larsson NG

Molecular control of mitochondrial function in preimplantation mouse embryos.
Molecular reproduction and development71(4)405-13(2005 Aug)
Thundathil J,Filion F,Smith LC

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Tfb1m cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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