gRNAs
The following Crybg3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Crybg3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OMu04692 | |
| Clone ID Related Accession (Same CDS sequence) | NM_174848.3 | |
| Accession Version | NM_174848.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3717bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-09-21 | |
| Organism | Mus musculus(house mouse) | |
| Product | beta/gamma crystallin domain-containing protein 3 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC043118.1 and AK162506.1. On Apr 28, 2010 this sequence version replaced NM_174848.2. Sequence Note: This RefSeq uses the 5'-most in-frame start codon that is known from available transcript data. It should be noted that the reading frame is open further upstream, and it is therefore possible that the protein may be N-terminally truncated. However, there is currently insufficient evidence in any species to further extend the coding region. ##Evidence-Data-START## Transcript exon combination :: BC043118.1, SRR1660821.165109.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## | |
1 | ATGGTACCTG CCGAACTAGA AATGGAAAGT ATTTATCCTA AGGACTCTGA AGGAGATACT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_777273.2 |
| CDS | 6..3722 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_174848.3 |
1 | ATGGTACCTG CCGAACTAGA AATGGAAAGT ATTTATCCTA AGGACTCTGA AGGAGATACT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu52094 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006522095.3 , NM_174848.4 | |
| Accession Version | NM_174848.4 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 8859bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-10-24 | |
| Organism | Mus musculus(house mouse) | |
| Product | beta/gamma crystallin domain-containing protein 3 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC154473.2 and AC154854.2. On or before Feb 20, 2019 this sequence version replaced XM_006522095.3, NM_174848.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGTCCGGCG GCCGCAGGAG GGGCAGCGCC CCCTGGCACA GCTTCTCTCG GTTCTTCGCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_777273.3 |
| CDS | 155..9013 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_174848.4 |
1 | ATGTCCGGCG GCCGCAGGAG GGGCAGCGCC CCCTGGCACA GCTTCTCTCG GTTCTTCGCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu52094 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006522095.3 , NM_174848.4 | |
| Accession Version | XM_006522095.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 8859bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-06-21 | |
| Organism | Mus musculus(house mouse) | |
| Product | beta/gamma crystallin domain-containing protein 3 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006522095.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGTCCGGCG GCCGCAGGAG GGGCAGCGCC CCCTGGCACA GCTTCTCTCG GTTCTTCGCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006522158.1 |
| CDS | 617..9475 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006522095.3 |
1 | ATGTCCGGCG GCCGCAGGAG GGGCAGCGCC CCCTGGCACA GCTTCTCTCG GTTCTTCGCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Aggregation-prone near-native intermediate formation during unfolding of a structurally similar nonlenticular ??-crystallin domain. |
BayGenomics: a resource of insertional mutations in mouse embryonic stem cells. |
