Wrn cDNA ORF clone, Mus musculus(house mouse)

The following Wrn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Wrn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu21413 NM_011721.4
Latest version!
Mus musculus Werner syndrome RecQ like helicase (Wrn), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 16 $727.30
$1039.00
OMu21413 NM_001122822.1
Latest version!
Mus musculus Werner syndrome RecQ like helicase (Wrn), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 16 $727.30
$1039.00
OMu21413 XM_017312662.2
Latest version!
Mus musculus Werner syndrome RecQ like helicase (Wrn), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu21413 XM_006509091.4
Latest version!
Mus musculus Werner syndrome RecQ like helicase (Wrn), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu40624 XM_006509093.4
Latest version!
Mus musculus Werner syndrome RecQ like helicase (Wrn), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
View Old Accession Versions >>
OMu40624 XM_006509093.3 Mus musculus Werner syndrome homolog (human) (Wrn), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OMu21413 XM_006509091.2 Mus musculus Werner syndrome homolog (human) (Wrn), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 16 $727.30
$1039.00
OMu21413 XM_006509091.3 Mus musculus Werner syndrome homolog (human) (Wrn), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 16 $727.30
$1039.00
OMu21413 XM_017312662.1 Mus musculus Werner syndrome homolog (human) (Wrn), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 16 $727.30
$1039.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu21413
    Clone ID Related Accession (Same CDS sequence) NM_011721.4 , XM_006509091.2 , XM_006509091.3 , XM_017312662.1 , XM_006509091.4 , XM_017312662.2 , NM_001122822.1
    Accession Version NM_011721.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4206bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-23
    Organism Mus musculus(house mouse)
    Product Werner syndrome ATP-dependent helicase homolog
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK171490.1, BC050921.1, AK052466.1 and AK162801.1. On Mar 25, 2008 this sequence version replaced NM_011721.3. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF091215.1, D86526.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGAAACCA CTTCACTACA GCGGAAATTT CCAGAATGGA TGTCTATGCA GAGTCAAAGA 
    TGTGCTACAG AAGAAAAGGC CTGCGTTCAG AAGAGTGTTC TTGAAGATAA CCTCCCATTC
    TTAGAATTCC CTGGATCCAT TGTTTACAGT TATGAAGCTA GTGATTGCTC CTTCCTGTCT
    GAAGACATTA GCATGCGTCT GTCTGATGGC GATGTGGTGG GATTTGACAT GGAATGGCCG
    CCCATATACA AGCCAGGGAA AAGAAGCAGA GTCGCAGTGA TCCAGTTGTG TGTGTCTGAG
    AGCAAATGTT ACTTGTTTCA CATTTCTTCC ATGTCAGTTT TCCCCCAGGG ATTAAAAATG
    TTACTAGAAA ACAAATCAAT TAAGAAGGCA GGGGTTGGGA TTGAAGGGGA CCAGTGGAAA
    CTTCTGCGTG ATTTTGACGT CAAGTTGGAG AGTTTTGTGG AGCTGACGGA TGTTGCCAAT
    GAAAAGTTGA AGTGCGCAGA GACCTGGAGC CTCAATGGTC TGGTTAAACA CGTCTTAGGG
    AAACAACTTT TGAAAGACAA GTCCATCCGC TGCAGCAATT GGAGTAATTT CCCCCTCACT
    GAGGACCAGA AACTGTATGC AGCCACTGAT GCTTATGCTG GTCTTATCAT CTATCAAAAA
    TTAGGAAATT TGGGTGATAC TGCGCAAGTG TTTGCTCTAA ATAAAGCAGA GGAAAACCTA
    CCTCTGGAGA TGAAGAAACA GTTGAATTCA ATCTCCGAAG AAATGAGGGA CCTAGCCAAT
    CGTTTTCCTG TCACTTGCAG AAATTTGGAA ACTCTCCAGA GGGTTCCTGT AATATTGAAG
    AGTATTTCAG AAAATCTCTG TTCATTGAGA AAAGTGATCT GTGGTCCTAC AAACACTGAG
    ACTAGACTGA AGCCGGGCAG TAGTTTTAAT TTACTGTCAT CAGAGGATTC AGCTGCTGCT
    GGAGAAAAAG AGAAACAGAT TGGAAAACAT AGTACTTTTG CTAAAATTAA AGAAGAACCA
    TGGGACCCAG AACTTGACAG TTTAGTGAAG CAAGAGGAGG TTGATGTATT TAGAAATCAA
    GTGAAGCAAG AAAAAGGTGA ATCTGAAAAT GAAATAGAAG ACAATCTGTT GAGAGAAGAT
    ATGGAAAGAA CTTGTGTGAT TCCTAGTATT TCAGAAAATG AACTCCAAGA TTTGGAACAG
    CAAGCTAAAG AAGAAAAATA TAATGATGTT TCTCACCAAC TTTCTGAGCA TTTATCTCCC
    AATGATGATG AGAATGACTC CTCCTATATA ATTGAAAGTG ATGAAGATTT GGAAATGGAG
    ATGCTGAAGT CTTTAGAAAA CCTAAATAGT GACGTGGTGG AACCCACTCA CTCTACATGG
    TTGGAAATGG GAACCAATGG GCGTCTTCCT CCTGAGGAGG AAGATGGACA CGGAAATGAA
    GCCATCAAAG AGGAGCAGGA AGAAGAGGAC CATTTATTGC CGGAACCCAA CGCAAAGCAA
    ATTAATTGCC TCAAGACCTA TTTCGGACAC AGCAGTTTTA AACCGGTTCA GTGGAAAGTC
    ATCCATTCTG TATTAGAAGA GAGAAGAGAT AATGTTGTTG TCATGGCAAC TGGATATGGG
    AAGAGTCTGT GCTTCCAGTA TCCGCCTGTT TATACAGGCA AGATTGGCAT TGTCATTTCA
    CCTCTCATTT CCTTAATGGA AGACCAAGTC CTCCAGCTTG AGCTGTCCAA TGTTCCAGCC
    TGTTTACTTG GATCTGCACA GTCAAAAAAT ATTCTAGGAG ATGTTAAATT AGGCAAATAT
    AGGGTCATCT ACATAACTCC AGAGTTCTGT TCTGGTAACT TGGATCTACT CCAGCAACTT
    GACTCTAGTA TTGGCATCAC TCTCATTGCT GTGGATGAGG CTCACTGCAT TTCAGAGTGG
    GGCCATGATT TCAGAAGTTC ATTCAGGATG CTGGGCTCTC TTAAAACAGC GCTCCCATTG
    GTTCCAGTCA TTGCACTCTC CGCTACTGCA AGCTCTTCCA TCCGGGAAGA CATTATAAGC
    TGCTTAAACC TGAAAGACCC TCAGATCACC TGCACTGGAT TTGATCGGCC AAATCTGTAC
    TTAGAAGTTG GACGGAAAAC AGGGAACATC CTTCAGGATC TAAAGCCGTT TCTCGTCCGA
    AAGGCAAGTT CTGCCTGGGA ATTTGAAGGT CCAACCATCA TCTATTGTCC TTCGAGAAAA
    ATGACAGAAC AAGTTACTGC TGAACTTGGG AAACTGAACT TAGCCTGCAG AACATACCAC
    GCTGGCATGA AAATTAGCGA AAGGAAGGAC GTTCATCATA GGTTCCTGAG AGATGAAATT
    CAGTGTGTTG TAGCTACTGT AGCTTTTGGA ATGGGCATTA ATAAAGCTGA CATTCGCAAA
    GTTATTCATT ATGGTGCGCC TAAGGAAATG GAATCCTATT ACCAGGAAAT TGGTAGAGCT
    GGCCGGGATG GACTTCAGAG TTCCTGTCAC TTGCTCTGGG CTCCAGCAGA CTTTAACACA
    TCCAGGAATC TCCTTATTGA GATTCACGAT GAAAAGTTCC GGTTATATAA ATTAAAGATG
    ATGGTAAAGA TGGAAAAATA CCTTCACTCC AGTCAGTGTA GGCGACGAAT CATCTTGTCC
    CATTTTGAGG ACAAATGTCT GCAGAAGGCC TCCTTGGACA TTATGGGAAC TGAAAAATGC
    TGTGATAATT GCAGGCCCAG GCTGAATCAT TGCCTTACTG CTAACAACTC AGAGGACGCA
    TCCCAAGACT TTGGGCCACA AGCATTCCAG CTACTGTCTG CTGTGGACAT CCTGCAGGAG
    AAATTTGGAA TTGGGATTCC GATCTTATTT CTCCGAGGAT CTAATTCTCA GCGTCTTCCT
    GATAAATATC GGGGTCACAG GCTCTTTGGT GCTGGAAAGG AGCAAGCAGA AAGTTGGTGG
    AAGACCCTTT CTCACCATCT CATAGCTGAA GGATTCTTGG TAGAAGTTCC CAAGGAAAAC
    AAATATATAA AGACATGTTC CCTCACAAAA AAGGGTAGAA AGTGGCTTGG AGAAGCCAGT
    TCGCAGTCTC CTCCGAGCCT TCTCCTTCAA GCTAATGAAG AGATGTTTCC AAGGAAAGTT
    CTGCTACCAA GTTCTAATCC TGTATCTCCA GAAACGACGC AACATTCCTC TAATCAAAAC
    CCAGCTGGAT TAACTACCAA GCAGTCTAAT TTGGAGAGAA CGCATTCTTA CAAAGTGCCT
    GAGAAAGTTT CTTCTGGGAC TAACATTCCT AAAAAAAGTG CCGTGATGCC GTCACCAGGA
    ACATCTTCCA GCCCCTTAGA ACCTGCCATC TCAGCCCAAG AGCTGGACGC TCGGACTGGG
    CTATATGCCA GGCTGGTGGA AGCAAGGCAG AAACACGCTA ATAAGATGGA TGTACCTCCA
    GCTATTTTAG CAACAAACAA GGTTCTGCTG GACATGGCTA AAATGAGACC GACTACTGTT
    GAAAACATGA AACAGATCGA CGGTGTCTCT GAAGGCAAAG CTGCTCTGTT GGCCCCTCTG
    TTGGAAGTCA TCAAACATTT CTGTCAAGTA ACTAGTGTTC AGACAGACCT CCTTTCCAGT
    GCCAAACCTC ACAAGGAACA GGAGAAAAGT CAGGAGATGG AAAAGAAAGA CTGCTCACTC
    CCCCAGTCTG TGGCCGTCAC ATACACTCTA TTCCAGGAAA AGAAAATGCC CTTACACAGC
    ATAGCTGAGA ACAGGCTCCT GCCTCTCACA GCAGCCGGCA TGCACTTAGC CCAGGCGGTG
    AAAGCCGGCT ACCCCCTGGA TATGGAGCGA GCTGGCCTGA CCCCAGAGAC TTGGAAGATT
    ATTATGGATG TCATCCGAAA CCCTCCCATC AACTCAGATA TGTATAAAGT TAAACTCATC
    AGAATGTTAG TTCCTGAAAA CTTAGACACG TACCTCATCC ACATGGCGAT TGAGATTCTT
    CAGAGTGGTT CCGACAGCAG AACCCAGCCT CCTTGTGATT CCAGCAGGAA GAGGCGTTTC
    CCCAGCTCTG CAGAGAGTTG TGAGAGCTGT AAGGAGAGCA AAGAGGCGGT CACCGAGACC
    AAGGCATCAT CTTCAGAGTC AAAGAGAAAA TTACCCGAGT GGTTTGCCAA AGGAAATGTG
    CCCTCAGCTG ATACCGGCAG CTCATCATCA ATGGCCAAGA CCAAAAAGAA AGGTCTCTTT
    AGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_035851.3
    CDS214..4419
    Translation

    Target ORF information:

    RefSeq Version NM_011721.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus Werner syndrome RecQ like helicase (Wrn), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_011721.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGAAACCA CTTCACTACA GCGGAAATTT CCAGAATGGA TGTCTATGCA GAGTCAAAGA 
    TGTGCTACAG AAGAAAAGGC CTGCGTTCAG AAGAGTGTTC TTGAAGATAA CCTCCCATTC
    TTAGAATTCC CTGGATCCAT TGTTTACAGT TATGAAGCTA GTGATTGCTC CTTCCTGTCT
    GAAGACATTA GCATGCGTCT GTCTGATGGC GATGTGGTGG GATTTGACAT GGAATGGCCG
    CCCATATACA AGCCAGGGAA AAGAAGCAGA GTCGCAGTGA TCCAGTTGTG TGTGTCTGAG
    AGCAAATGTT ACTTGTTTCA CATTTCTTCC ATGTCAGTTT TCCCCCAGGG ATTAAAAATG
    TTACTAGAAA ACAAATCAAT TAAGAAGGCA GGGGTTGGGA TTGAAGGGGA CCAGTGGAAA
    CTTCTGCGTG ATTTTGACGT CAAGTTGGAG AGTTTTGTGG AGCTGACGGA TGTTGCCAAT
    GAAAAGTTGA AGTGCGCAGA GACCTGGAGC CTCAATGGTC TGGTTAAACA CGTCTTAGGG
    AAACAACTTT TGAAAGACAA GTCCATCCGC TGCAGCAATT GGAGTAATTT CCCCCTCACT
    GAGGACCAGA AACTGTATGC AGCCACTGAT GCTTATGCTG GTCTTATCAT CTATCAAAAA
    TTAGGAAATT TGGGTGATAC TGCGCAAGTG TTTGCTCTAA ATAAAGCAGA GGAAAACCTA
    CCTCTGGAGA TGAAGAAACA GTTGAATTCA ATCTCCGAAG AAATGAGGGA CCTAGCCAAT
    CGTTTTCCTG TCACTTGCAG AAATTTGGAA ACTCTCCAGA GGGTTCCTGT AATATTGAAG
    AGTATTTCAG AAAATCTCTG TTCATTGAGA AAAGTGATCT GTGGTCCTAC AAACACTGAG
    ACTAGACTGA AGCCGGGCAG TAGTTTTAAT TTACTGTCAT CAGAGGATTC AGCTGCTGCT
    GGAGAAAAAG AGAAACAGAT TGGAAAACAT AGTACTTTTG CTAAAATTAA AGAAGAACCA
    TGGGACCCAG AACTTGACAG TTTAGTGAAG CAAGAGGAGG TTGATGTATT TAGAAATCAA
    GTGAAGCAAG AAAAAGGTGA ATCTGAAAAT GAAATAGAAG ACAATCTGTT GAGAGAAGAT
    ATGGAAAGAA CTTGTGTGAT TCCTAGTATT TCAGAAAATG AACTCCAAGA TTTGGAACAG
    CAAGCTAAAG AAGAAAAATA TAATGATGTT TCTCACCAAC TTTCTGAGCA TTTATCTCCC
    AATGATGATG AGAATGACTC CTCCTATATA ATTGAAAGTG ATGAAGATTT GGAAATGGAG
    ATGCTGAAGT CTTTAGAAAA CCTAAATAGT GACGTGGTGG AACCCACTCA CTCTACATGG
    TTGGAAATGG GAACCAATGG GCGTCTTCCT CCTGAGGAGG AAGATGGACA CGGAAATGAA
    GCCATCAAAG AGGAGCAGGA AGAAGAGGAC CATTTATTGC CGGAACCCAA CGCAAAGCAA
    ATTAATTGCC TCAAGACCTA TTTCGGACAC AGCAGTTTTA AACCGGTTCA GTGGAAAGTC
    ATCCATTCTG TATTAGAAGA GAGAAGAGAT AATGTTGTTG TCATGGCAAC TGGATATGGG
    AAGAGTCTGT GCTTCCAGTA TCCGCCTGTT TATACAGGCA AGATTGGCAT TGTCATTTCA
    CCTCTCATTT CCTTAATGGA AGACCAAGTC CTCCAGCTTG AGCTGTCCAA TGTTCCAGCC
    TGTTTACTTG GATCTGCACA GTCAAAAAAT ATTCTAGGAG ATGTTAAATT AGGCAAATAT
    AGGGTCATCT ACATAACTCC AGAGTTCTGT TCTGGTAACT TGGATCTACT CCAGCAACTT
    GACTCTAGTA TTGGCATCAC TCTCATTGCT GTGGATGAGG CTCACTGCAT TTCAGAGTGG
    GGCCATGATT TCAGAAGTTC ATTCAGGATG CTGGGCTCTC TTAAAACAGC GCTCCCATTG
    GTTCCAGTCA TTGCACTCTC CGCTACTGCA AGCTCTTCCA TCCGGGAAGA CATTATAAGC
    TGCTTAAACC TGAAAGACCC TCAGATCACC TGCACTGGAT TTGATCGGCC AAATCTGTAC
    TTAGAAGTTG GACGGAAAAC AGGGAACATC CTTCAGGATC TAAAGCCGTT TCTCGTCCGA
    AAGGCAAGTT CTGCCTGGGA ATTTGAAGGT CCAACCATCA TCTATTGTCC TTCGAGAAAA
    ATGACAGAAC AAGTTACTGC TGAACTTGGG AAACTGAACT TAGCCTGCAG AACATACCAC
    GCTGGCATGA AAATTAGCGA AAGGAAGGAC GTTCATCATA GGTTCCTGAG AGATGAAATT
    CAGTGTGTTG TAGCTACTGT AGCTTTTGGA ATGGGCATTA ATAAAGCTGA CATTCGCAAA
    GTTATTCATT ATGGTGCGCC TAAGGAAATG GAATCCTATT ACCAGGAAAT TGGTAGAGCT
    GGCCGGGATG GACTTCAGAG TTCCTGTCAC TTGCTCTGGG CTCCAGCAGA CTTTAACACA
    TCCAGGAATC TCCTTATTGA GATTCACGAT GAAAAGTTCC GGTTATATAA ATTAAAGATG
    ATGGTAAAGA TGGAAAAATA CCTTCACTCC AGTCAGTGTA GGCGACGAAT CATCTTGTCC
    CATTTTGAGG ACAAATGTCT GCAGAAGGCC TCCTTGGACA TTATGGGAAC TGAAAAATGC
    TGTGATAATT GCAGGCCCAG GCTGAATCAT TGCCTTACTG CTAACAACTC AGAGGACGCA
    TCCCAAGACT TTGGGCCACA AGCATTCCAG CTACTGTCTG CTGTGGACAT CCTGCAGGAG
    AAATTTGGAA TTGGGATTCC GATCTTATTT CTCCGAGGAT CTAATTCTCA GCGTCTTCCT
    GATAAATATC GGGGTCACAG GCTCTTTGGT GCTGGAAAGG AGCAAGCAGA AAGTTGGTGG
    AAGACCCTTT CTCACCATCT CATAGCTGAA GGATTCTTGG TAGAAGTTCC CAAGGAAAAC
    AAATATATAA AGACATGTTC CCTCACAAAA AAGGGTAGAA AGTGGCTTGG AGAAGCCAGT
    TCGCAGTCTC CTCCGAGCCT TCTCCTTCAA GCTAATGAAG AGATGTTTCC AAGGAAAGTT
    CTGCTACCAA GTTCTAATCC TGTATCTCCA GAAACGACGC AACATTCCTC TAATCAAAAC
    CCAGCTGGAT TAACTACCAA GCAGTCTAAT TTGGAGAGAA CGCATTCTTA CAAAGTGCCT
    GAGAAAGTTT CTTCTGGGAC TAACATTCCT AAAAAAAGTG CCGTGATGCC GTCACCAGGA
    ACATCTTCCA GCCCCTTAGA ACCTGCCATC TCAGCCCAAG AGCTGGACGC TCGGACTGGG
    CTATATGCCA GGCTGGTGGA AGCAAGGCAG AAACACGCTA ATAAGATGGA TGTACCTCCA
    GCTATTTTAG CAACAAACAA GGTTCTGCTG GACATGGCTA AAATGAGACC GACTACTGTT
    GAAAACATGA AACAGATCGA CGGTGTCTCT GAAGGCAAAG CTGCTCTGTT GGCCCCTCTG
    TTGGAAGTCA TCAAACATTT CTGTCAAGTA ACTAGTGTTC AGACAGACCT CCTTTCCAGT
    GCCAAACCTC ACAAGGAACA GGAGAAAAGT CAGGAGATGG AAAAGAAAGA CTGCTCACTC
    CCCCAGTCTG TGGCCGTCAC ATACACTCTA TTCCAGGAAA AGAAAATGCC CTTACACAGC
    ATAGCTGAGA ACAGGCTCCT GCCTCTCACA GCAGCCGGCA TGCACTTAGC CCAGGCGGTG
    AAAGCCGGCT ACCCCCTGGA TATGGAGCGA GCTGGCCTGA CCCCAGAGAC TTGGAAGATT
    ATTATGGATG TCATCCGAAA CCCTCCCATC AACTCAGATA TGTATAAAGT TAAACTCATC
    AGAATGTTAG TTCCTGAAAA CTTAGACACG TACCTCATCC ACATGGCGAT TGAGATTCTT
    CAGAGTGGTT CCGACAGCAG AACCCAGCCT CCTTGTGATT CCAGCAGGAA GAGGCGTTTC
    CCCAGCTCTG CAGAGAGTTG TGAGAGCTGT AAGGAGAGCA AAGAGGCGGT CACCGAGACC
    AAGGCATCAT CTTCAGAGTC AAAGAGAAAA TTACCCGAGT GGTTTGCCAA AGGAAATGTG
    CCCTCAGCTG ATACCGGCAG CTCATCATCA ATGGCCAAGA CCAAAAAGAA AGGTCTCTTT
    AGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu21413
    Clone ID Related Accession (Same CDS sequence) NM_011721.4 , XM_006509091.2 , XM_006509091.3 , XM_017312662.1 , XM_006509091.4 , XM_017312662.2 , NM_001122822.1
    Accession Version NM_001122822.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4206bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-23
    Organism Mus musculus(house mouse)
    Product Werner syndrome ATP-dependent helicase homolog
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK171490.1, BC050921.1, AK052466.1 and AK162801.1. On Mar 2, 2012 this sequence version replaced XM_003689430.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AF091215.1, BC050921.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGAAACCA CTTCACTACA GCGGAAATTT CCAGAATGGA TGTCTATGCA GAGTCAAAGA 
    TGTGCTACAG AAGAAAAGGC CTGCGTTCAG AAGAGTGTTC TTGAAGATAA CCTCCCATTC
    TTAGAATTCC CTGGATCCAT TGTTTACAGT TATGAAGCTA GTGATTGCTC CTTCCTGTCT
    GAAGACATTA GCATGCGTCT GTCTGATGGC GATGTGGTGG GATTTGACAT GGAATGGCCG
    CCCATATACA AGCCAGGGAA AAGAAGCAGA GTCGCAGTGA TCCAGTTGTG TGTGTCTGAG
    AGCAAATGTT ACTTGTTTCA CATTTCTTCC ATGTCAGTTT TCCCCCAGGG ATTAAAAATG
    TTACTAGAAA ACAAATCAAT TAAGAAGGCA GGGGTTGGGA TTGAAGGGGA CCAGTGGAAA
    CTTCTGCGTG ATTTTGACGT CAAGTTGGAG AGTTTTGTGG AGCTGACGGA TGTTGCCAAT
    GAAAAGTTGA AGTGCGCAGA GACCTGGAGC CTCAATGGTC TGGTTAAACA CGTCTTAGGG
    AAACAACTTT TGAAAGACAA GTCCATCCGC TGCAGCAATT GGAGTAATTT CCCCCTCACT
    GAGGACCAGA AACTGTATGC AGCCACTGAT GCTTATGCTG GTCTTATCAT CTATCAAAAA
    TTAGGAAATT TGGGTGATAC TGCGCAAGTG TTTGCTCTAA ATAAAGCAGA GGAAAACCTA
    CCTCTGGAGA TGAAGAAACA GTTGAATTCA ATCTCCGAAG AAATGAGGGA CCTAGCCAAT
    CGTTTTCCTG TCACTTGCAG AAATTTGGAA ACTCTCCAGA GGGTTCCTGT AATATTGAAG
    AGTATTTCAG AAAATCTCTG TTCATTGAGA AAAGTGATCT GTGGTCCTAC AAACACTGAG
    ACTAGACTGA AGCCGGGCAG TAGTTTTAAT TTACTGTCAT CAGAGGATTC AGCTGCTGCT
    GGAGAAAAAG AGAAACAGAT TGGAAAACAT AGTACTTTTG CTAAAATTAA AGAAGAACCA
    TGGGACCCAG AACTTGACAG TTTAGTGAAG CAAGAGGAGG TTGATGTATT TAGAAATCAA
    GTGAAGCAAG AAAAAGGTGA ATCTGAAAAT GAAATAGAAG ACAATCTGTT GAGAGAAGAT
    ATGGAAAGAA CTTGTGTGAT TCCTAGTATT TCAGAAAATG AACTCCAAGA TTTGGAACAG
    CAAGCTAAAG AAGAAAAATA TAATGATGTT TCTCACCAAC TTTCTGAGCA TTTATCTCCC
    AATGATGATG AGAATGACTC CTCCTATATA ATTGAAAGTG ATGAAGATTT GGAAATGGAG
    ATGCTGAAGT CTTTAGAAAA CCTAAATAGT GACGTGGTGG AACCCACTCA CTCTACATGG
    TTGGAAATGG GAACCAATGG GCGTCTTCCT CCTGAGGAGG AAGATGGACA CGGAAATGAA
    GCCATCAAAG AGGAGCAGGA AGAAGAGGAC CATTTATTGC CGGAACCCAA CGCAAAGCAA
    ATTAATTGCC TCAAGACCTA TTTCGGACAC AGCAGTTTTA AACCGGTTCA GTGGAAAGTC
    ATCCATTCTG TATTAGAAGA GAGAAGAGAT AATGTTGTTG TCATGGCAAC TGGATATGGG
    AAGAGTCTGT GCTTCCAGTA TCCGCCTGTT TATACAGGCA AGATTGGCAT TGTCATTTCA
    CCTCTCATTT CCTTAATGGA AGACCAAGTC CTCCAGCTTG AGCTGTCCAA TGTTCCAGCC
    TGTTTACTTG GATCTGCACA GTCAAAAAAT ATTCTAGGAG ATGTTAAATT AGGCAAATAT
    AGGGTCATCT ACATAACTCC AGAGTTCTGT TCTGGTAACT TGGATCTACT CCAGCAACTT
    GACTCTAGTA TTGGCATCAC TCTCATTGCT GTGGATGAGG CTCACTGCAT TTCAGAGTGG
    GGCCATGATT TCAGAAGTTC ATTCAGGATG CTGGGCTCTC TTAAAACAGC GCTCCCATTG
    GTTCCAGTCA TTGCACTCTC CGCTACTGCA AGCTCTTCCA TCCGGGAAGA CATTATAAGC
    TGCTTAAACC TGAAAGACCC TCAGATCACC TGCACTGGAT TTGATCGGCC AAATCTGTAC
    TTAGAAGTTG GACGGAAAAC AGGGAACATC CTTCAGGATC TAAAGCCGTT TCTCGTCCGA
    AAGGCAAGTT CTGCCTGGGA ATTTGAAGGT CCAACCATCA TCTATTGTCC TTCGAGAAAA
    ATGACAGAAC AAGTTACTGC TGAACTTGGG AAACTGAACT TAGCCTGCAG AACATACCAC
    GCTGGCATGA AAATTAGCGA AAGGAAGGAC GTTCATCATA GGTTCCTGAG AGATGAAATT
    CAGTGTGTTG TAGCTACTGT AGCTTTTGGA ATGGGCATTA ATAAAGCTGA CATTCGCAAA
    GTTATTCATT ATGGTGCGCC TAAGGAAATG GAATCCTATT ACCAGGAAAT TGGTAGAGCT
    GGCCGGGATG GACTTCAGAG TTCCTGTCAC TTGCTCTGGG CTCCAGCAGA CTTTAACACA
    TCCAGGAATC TCCTTATTGA GATTCACGAT GAAAAGTTCC GGTTATATAA ATTAAAGATG
    ATGGTAAAGA TGGAAAAATA CCTTCACTCC AGTCAGTGTA GGCGACGAAT CATCTTGTCC
    CATTTTGAGG ACAAATGTCT GCAGAAGGCC TCCTTGGACA TTATGGGAAC TGAAAAATGC
    TGTGATAATT GCAGGCCCAG GCTGAATCAT TGCCTTACTG CTAACAACTC AGAGGACGCA
    TCCCAAGACT TTGGGCCACA AGCATTCCAG CTACTGTCTG CTGTGGACAT CCTGCAGGAG
    AAATTTGGAA TTGGGATTCC GATCTTATTT CTCCGAGGAT CTAATTCTCA GCGTCTTCCT
    GATAAATATC GGGGTCACAG GCTCTTTGGT GCTGGAAAGG AGCAAGCAGA AAGTTGGTGG
    AAGACCCTTT CTCACCATCT CATAGCTGAA GGATTCTTGG TAGAAGTTCC CAAGGAAAAC
    AAATATATAA AGACATGTTC CCTCACAAAA AAGGGTAGAA AGTGGCTTGG AGAAGCCAGT
    TCGCAGTCTC CTCCGAGCCT TCTCCTTCAA GCTAATGAAG AGATGTTTCC AAGGAAAGTT
    CTGCTACCAA GTTCTAATCC TGTATCTCCA GAAACGACGC AACATTCCTC TAATCAAAAC
    CCAGCTGGAT TAACTACCAA GCAGTCTAAT TTGGAGAGAA CGCATTCTTA CAAAGTGCCT
    GAGAAAGTTT CTTCTGGGAC TAACATTCCT AAAAAAAGTG CCGTGATGCC GTCACCAGGA
    ACATCTTCCA GCCCCTTAGA ACCTGCCATC TCAGCCCAAG AGCTGGACGC TCGGACTGGG
    CTATATGCCA GGCTGGTGGA AGCAAGGCAG AAACACGCTA ATAAGATGGA TGTACCTCCA
    GCTATTTTAG CAACAAACAA GGTTCTGCTG GACATGGCTA AAATGAGACC GACTACTGTT
    GAAAACATGA AACAGATCGA CGGTGTCTCT GAAGGCAAAG CTGCTCTGTT GGCCCCTCTG
    TTGGAAGTCA TCAAACATTT CTGTCAAGTA ACTAGTGTTC AGACAGACCT CCTTTCCAGT
    GCCAAACCTC ACAAGGAACA GGAGAAAAGT CAGGAGATGG AAAAGAAAGA CTGCTCACTC
    CCCCAGTCTG TGGCCGTCAC ATACACTCTA TTCCAGGAAA AGAAAATGCC CTTACACAGC
    ATAGCTGAGA ACAGGCTCCT GCCTCTCACA GCAGCCGGCA TGCACTTAGC CCAGGCGGTG
    AAAGCCGGCT ACCCCCTGGA TATGGAGCGA GCTGGCCTGA CCCCAGAGAC TTGGAAGATT
    ATTATGGATG TCATCCGAAA CCCTCCCATC AACTCAGATA TGTATAAAGT TAAACTCATC
    AGAATGTTAG TTCCTGAAAA CTTAGACACG TACCTCATCC ACATGGCGAT TGAGATTCTT
    CAGAGTGGTT CCGACAGCAG AACCCAGCCT CCTTGTGATT CCAGCAGGAA GAGGCGTTTC
    CCCAGCTCTG CAGAGAGTTG TGAGAGCTGT AAGGAGAGCA AAGAGGCGGT CACCGAGACC
    AAGGCATCAT CTTCAGAGTC AAAGAGAAAA TTACCCGAGT GGTTTGCCAA AGGAAATGTG
    CCCTCAGCTG ATACCGGCAG CTCATCATCA ATGGCCAAGA CCAAAAAGAA AGGTCTCTTT
    AGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001116294.1
    CDS104..4309
    Translation

    Target ORF information:

    RefSeq Version NM_001122822.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus Werner syndrome RecQ like helicase (Wrn), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001122822.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGAAACCA CTTCACTACA GCGGAAATTT CCAGAATGGA TGTCTATGCA GAGTCAAAGA 
    TGTGCTACAG AAGAAAAGGC CTGCGTTCAG AAGAGTGTTC TTGAAGATAA CCTCCCATTC
    TTAGAATTCC CTGGATCCAT TGTTTACAGT TATGAAGCTA GTGATTGCTC CTTCCTGTCT
    GAAGACATTA GCATGCGTCT GTCTGATGGC GATGTGGTGG GATTTGACAT GGAATGGCCG
    CCCATATACA AGCCAGGGAA AAGAAGCAGA GTCGCAGTGA TCCAGTTGTG TGTGTCTGAG
    AGCAAATGTT ACTTGTTTCA CATTTCTTCC ATGTCAGTTT TCCCCCAGGG ATTAAAAATG
    TTACTAGAAA ACAAATCAAT TAAGAAGGCA GGGGTTGGGA TTGAAGGGGA CCAGTGGAAA
    CTTCTGCGTG ATTTTGACGT CAAGTTGGAG AGTTTTGTGG AGCTGACGGA TGTTGCCAAT
    GAAAAGTTGA AGTGCGCAGA GACCTGGAGC CTCAATGGTC TGGTTAAACA CGTCTTAGGG
    AAACAACTTT TGAAAGACAA GTCCATCCGC TGCAGCAATT GGAGTAATTT CCCCCTCACT
    GAGGACCAGA AACTGTATGC AGCCACTGAT GCTTATGCTG GTCTTATCAT CTATCAAAAA
    TTAGGAAATT TGGGTGATAC TGCGCAAGTG TTTGCTCTAA ATAAAGCAGA GGAAAACCTA
    CCTCTGGAGA TGAAGAAACA GTTGAATTCA ATCTCCGAAG AAATGAGGGA CCTAGCCAAT
    CGTTTTCCTG TCACTTGCAG AAATTTGGAA ACTCTCCAGA GGGTTCCTGT AATATTGAAG
    AGTATTTCAG AAAATCTCTG TTCATTGAGA AAAGTGATCT GTGGTCCTAC AAACACTGAG
    ACTAGACTGA AGCCGGGCAG TAGTTTTAAT TTACTGTCAT CAGAGGATTC AGCTGCTGCT
    GGAGAAAAAG AGAAACAGAT TGGAAAACAT AGTACTTTTG CTAAAATTAA AGAAGAACCA
    TGGGACCCAG AACTTGACAG TTTAGTGAAG CAAGAGGAGG TTGATGTATT TAGAAATCAA
    GTGAAGCAAG AAAAAGGTGA ATCTGAAAAT GAAATAGAAG ACAATCTGTT GAGAGAAGAT
    ATGGAAAGAA CTTGTGTGAT TCCTAGTATT TCAGAAAATG AACTCCAAGA TTTGGAACAG
    CAAGCTAAAG AAGAAAAATA TAATGATGTT TCTCACCAAC TTTCTGAGCA TTTATCTCCC
    AATGATGATG AGAATGACTC CTCCTATATA ATTGAAAGTG ATGAAGATTT GGAAATGGAG
    ATGCTGAAGT CTTTAGAAAA CCTAAATAGT GACGTGGTGG AACCCACTCA CTCTACATGG
    TTGGAAATGG GAACCAATGG GCGTCTTCCT CCTGAGGAGG AAGATGGACA CGGAAATGAA
    GCCATCAAAG AGGAGCAGGA AGAAGAGGAC CATTTATTGC CGGAACCCAA CGCAAAGCAA
    ATTAATTGCC TCAAGACCTA TTTCGGACAC AGCAGTTTTA AACCGGTTCA GTGGAAAGTC
    ATCCATTCTG TATTAGAAGA GAGAAGAGAT AATGTTGTTG TCATGGCAAC TGGATATGGG
    AAGAGTCTGT GCTTCCAGTA TCCGCCTGTT TATACAGGCA AGATTGGCAT TGTCATTTCA
    CCTCTCATTT CCTTAATGGA AGACCAAGTC CTCCAGCTTG AGCTGTCCAA TGTTCCAGCC
    TGTTTACTTG GATCTGCACA GTCAAAAAAT ATTCTAGGAG ATGTTAAATT AGGCAAATAT
    AGGGTCATCT ACATAACTCC AGAGTTCTGT TCTGGTAACT TGGATCTACT CCAGCAACTT
    GACTCTAGTA TTGGCATCAC TCTCATTGCT GTGGATGAGG CTCACTGCAT TTCAGAGTGG
    GGCCATGATT TCAGAAGTTC ATTCAGGATG CTGGGCTCTC TTAAAACAGC GCTCCCATTG
    GTTCCAGTCA TTGCACTCTC CGCTACTGCA AGCTCTTCCA TCCGGGAAGA CATTATAAGC
    TGCTTAAACC TGAAAGACCC TCAGATCACC TGCACTGGAT TTGATCGGCC AAATCTGTAC
    TTAGAAGTTG GACGGAAAAC AGGGAACATC CTTCAGGATC TAAAGCCGTT TCTCGTCCGA
    AAGGCAAGTT CTGCCTGGGA ATTTGAAGGT CCAACCATCA TCTATTGTCC TTCGAGAAAA
    ATGACAGAAC AAGTTACTGC TGAACTTGGG AAACTGAACT TAGCCTGCAG AACATACCAC
    GCTGGCATGA AAATTAGCGA AAGGAAGGAC GTTCATCATA GGTTCCTGAG AGATGAAATT
    CAGTGTGTTG TAGCTACTGT AGCTTTTGGA ATGGGCATTA ATAAAGCTGA CATTCGCAAA
    GTTATTCATT ATGGTGCGCC TAAGGAAATG GAATCCTATT ACCAGGAAAT TGGTAGAGCT
    GGCCGGGATG GACTTCAGAG TTCCTGTCAC TTGCTCTGGG CTCCAGCAGA CTTTAACACA
    TCCAGGAATC TCCTTATTGA GATTCACGAT GAAAAGTTCC GGTTATATAA ATTAAAGATG
    ATGGTAAAGA TGGAAAAATA CCTTCACTCC AGTCAGTGTA GGCGACGAAT CATCTTGTCC
    CATTTTGAGG ACAAATGTCT GCAGAAGGCC TCCTTGGACA TTATGGGAAC TGAAAAATGC
    TGTGATAATT GCAGGCCCAG GCTGAATCAT TGCCTTACTG CTAACAACTC AGAGGACGCA
    TCCCAAGACT TTGGGCCACA AGCATTCCAG CTACTGTCTG CTGTGGACAT CCTGCAGGAG
    AAATTTGGAA TTGGGATTCC GATCTTATTT CTCCGAGGAT CTAATTCTCA GCGTCTTCCT
    GATAAATATC GGGGTCACAG GCTCTTTGGT GCTGGAAAGG AGCAAGCAGA AAGTTGGTGG
    AAGACCCTTT CTCACCATCT CATAGCTGAA GGATTCTTGG TAGAAGTTCC CAAGGAAAAC
    AAATATATAA AGACATGTTC CCTCACAAAA AAGGGTAGAA AGTGGCTTGG AGAAGCCAGT
    TCGCAGTCTC CTCCGAGCCT TCTCCTTCAA GCTAATGAAG AGATGTTTCC AAGGAAAGTT
    CTGCTACCAA GTTCTAATCC TGTATCTCCA GAAACGACGC AACATTCCTC TAATCAAAAC
    CCAGCTGGAT TAACTACCAA GCAGTCTAAT TTGGAGAGAA CGCATTCTTA CAAAGTGCCT
    GAGAAAGTTT CTTCTGGGAC TAACATTCCT AAAAAAAGTG CCGTGATGCC GTCACCAGGA
    ACATCTTCCA GCCCCTTAGA ACCTGCCATC TCAGCCCAAG AGCTGGACGC TCGGACTGGG
    CTATATGCCA GGCTGGTGGA AGCAAGGCAG AAACACGCTA ATAAGATGGA TGTACCTCCA
    GCTATTTTAG CAACAAACAA GGTTCTGCTG GACATGGCTA AAATGAGACC GACTACTGTT
    GAAAACATGA AACAGATCGA CGGTGTCTCT GAAGGCAAAG CTGCTCTGTT GGCCCCTCTG
    TTGGAAGTCA TCAAACATTT CTGTCAAGTA ACTAGTGTTC AGACAGACCT CCTTTCCAGT
    GCCAAACCTC ACAAGGAACA GGAGAAAAGT CAGGAGATGG AAAAGAAAGA CTGCTCACTC
    CCCCAGTCTG TGGCCGTCAC ATACACTCTA TTCCAGGAAA AGAAAATGCC CTTACACAGC
    ATAGCTGAGA ACAGGCTCCT GCCTCTCACA GCAGCCGGCA TGCACTTAGC CCAGGCGGTG
    AAAGCCGGCT ACCCCCTGGA TATGGAGCGA GCTGGCCTGA CCCCAGAGAC TTGGAAGATT
    ATTATGGATG TCATCCGAAA CCCTCCCATC AACTCAGATA TGTATAAAGT TAAACTCATC
    AGAATGTTAG TTCCTGAAAA CTTAGACACG TACCTCATCC ACATGGCGAT TGAGATTCTT
    CAGAGTGGTT CCGACAGCAG AACCCAGCCT CCTTGTGATT CCAGCAGGAA GAGGCGTTTC
    CCCAGCTCTG CAGAGAGTTG TGAGAGCTGT AAGGAGAGCA AAGAGGCGGT CACCGAGACC
    AAGGCATCAT CTTCAGAGTC AAAGAGAAAA TTACCCGAGT GGTTTGCCAA AGGAAATGTG
    CCCTCAGCTG ATACCGGCAG CTCATCATCA ATGGCCAAGA CCAAAAAGAA AGGTCTCTTT
    AGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu21413
    Clone ID Related Accession (Same CDS sequence) NM_011721.4 , XM_006509091.2 , XM_006509091.3 , XM_017312662.1 , XM_006509091.4 , XM_017312662.2 , NM_001122822.1
    Accession Version XM_006509091.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4206bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-02-08
    Organism Mus musculus(House mouse)
    Product Werner syndrome ATP-dependent helicase homolog isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGAAACCA CTTCACTACA GCGGAAATTT CCAGAATGGA TGTCTATGCA GAGTCAAAGA 
    TGTGCTACAG AAGAAAAGGC CTGCGTTCAG AAGAGTGTTC TTGAAGATAA CCTCCCATTC
    TTAGAATTCC CTGGATCCAT TGTTTACAGT TATGAAGCTA GTGATTGCTC CTTCCTGTCT
    GAAGACATTA GCATGCGTCT GTCTGATGGC GATGTGGTGG GATTTGACAT GGAATGGCCG
    CCCATATACA AGCCAGGGAA AAGAAGCAGA GTCGCAGTGA TCCAGTTGTG TGTGTCTGAG
    AGCAAATGTT ACTTGTTTCA CATTTCTTCC ATGTCAGTTT TCCCCCAGGG ATTAAAAATG
    TTACTAGAAA ACAAATCAAT TAAGAAGGCA GGGGTTGGGA TTGAAGGGGA CCAGTGGAAA
    CTTCTGCGTG ATTTTGACGT CAAGTTGGAG AGTTTTGTGG AGCTGACGGA TGTTGCCAAT
    GAAAAGTTGA AGTGCGCAGA GACCTGGAGC CTCAATGGTC TGGTTAAACA CGTCTTAGGG
    AAACAACTTT TGAAAGACAA GTCCATCCGC TGCAGCAATT GGAGTAATTT CCCCCTCACT
    GAGGACCAGA AACTGTATGC AGCCACTGAT GCTTATGCTG GTCTTATCAT CTATCAAAAA
    TTAGGAAATT TGGGTGATAC TGCGCAAGTG TTTGCTCTAA ATAAAGCAGA GGAAAACCTA
    CCTCTGGAGA TGAAGAAACA GTTGAATTCA ATCTCCGAAG AAATGAGGGA CCTAGCCAAT
    CGTTTTCCTG TCACTTGCAG AAATTTGGAA ACTCTCCAGA GGGTTCCTGT AATATTGAAG
    AGTATTTCAG AAAATCTCTG TTCATTGAGA AAAGTGATCT GTGGTCCTAC AAACACTGAG
    ACTAGACTGA AGCCGGGCAG TAGTTTTAAT TTACTGTCAT CAGAGGATTC AGCTGCTGCT
    GGAGAAAAAG AGAAACAGAT TGGAAAACAT AGTACTTTTG CTAAAATTAA AGAAGAACCA
    TGGGACCCAG AACTTGACAG TTTAGTGAAG CAAGAGGAGG TTGATGTATT TAGAAATCAA
    GTGAAGCAAG AAAAAGGTGA ATCTGAAAAT GAAATAGAAG ACAATCTGTT GAGAGAAGAT
    ATGGAAAGAA CTTGTGTGAT TCCTAGTATT TCAGAAAATG AACTCCAAGA TTTGGAACAG
    CAAGCTAAAG AAGAAAAATA TAATGATGTT TCTCACCAAC TTTCTGAGCA TTTATCTCCC
    AATGATGATG AGAATGACTC CTCCTATATA ATTGAAAGTG ATGAAGATTT GGAAATGGAG
    ATGCTGAAGT CTTTAGAAAA CCTAAATAGT GACGTGGTGG AACCCACTCA CTCTACATGG
    TTGGAAATGG GAACCAATGG GCGTCTTCCT CCTGAGGAGG AAGATGGACA CGGAAATGAA
    GCCATCAAAG AGGAGCAGGA AGAAGAGGAC CATTTATTGC CGGAACCCAA CGCAAAGCAA
    ATTAATTGCC TCAAGACCTA TTTCGGACAC AGCAGTTTTA AACCGGTTCA GTGGAAAGTC
    ATCCATTCTG TATTAGAAGA GAGAAGAGAT AATGTTGTTG TCATGGCAAC TGGATATGGG
    AAGAGTCTGT GCTTCCAGTA TCCGCCTGTT TATACAGGCA AGATTGGCAT TGTCATTTCA
    CCTCTCATTT CCTTAATGGA AGACCAAGTC CTCCAGCTTG AGCTGTCCAA TGTTCCAGCC
    TGTTTACTTG GATCTGCACA GTCAAAAAAT ATTCTAGGAG ATGTTAAATT AGGCAAATAT
    AGGGTCATCT ACATAACTCC AGAGTTCTGT TCTGGTAACT TGGATCTACT CCAGCAACTT
    GACTCTAGTA TTGGCATCAC TCTCATTGCT GTGGATGAGG CTCACTGCAT TTCAGAGTGG
    GGCCATGATT TCAGAAGTTC ATTCAGGATG CTGGGCTCTC TTAAAACAGC GCTCCCATTG
    GTTCCAGTCA TTGCACTCTC CGCTACTGCA AGCTCTTCCA TCCGGGAAGA CATTATAAGC
    TGCTTAAACC TGAAAGACCC TCAGATCACC TGCACTGGAT TTGATCGGCC AAATCTGTAC
    TTAGAAGTTG GACGGAAAAC AGGGAACATC CTTCAGGATC TAAAGCCGTT TCTCGTCCGA
    AAGGCAAGTT CTGCCTGGGA ATTTGAAGGT CCAACCATCA TCTATTGTCC TTCGAGAAAA
    ATGACAGAAC AAGTTACTGC TGAACTTGGG AAACTGAACT TAGCCTGCAG AACATACCAC
    GCTGGCATGA AAATTAGCGA AAGGAAGGAC GTTCATCATA GGTTCCTGAG AGATGAAATT
    CAGTGTGTTG TAGCTACTGT AGCTTTTGGA ATGGGCATTA ATAAAGCTGA CATTCGCAAA
    GTTATTCATT ATGGTGCGCC TAAGGAAATG GAATCCTATT ACCAGGAAAT TGGTAGAGCT
    GGCCGGGATG GACTTCAGAG TTCCTGTCAC TTGCTCTGGG CTCCAGCAGA CTTTAACACA
    TCCAGGAATC TCCTTATTGA GATTCACGAT GAAAAGTTCC GGTTATATAA ATTAAAGATG
    ATGGTAAAGA TGGAAAAATA CCTTCACTCC AGTCAGTGTA GGCGACGAAT CATCTTGTCC
    CATTTTGAGG ACAAATGTCT GCAGAAGGCC TCCTTGGACA TTATGGGAAC TGAAAAATGC
    TGTGATAATT GCAGGCCCAG GCTGAATCAT TGCCTTACTG CTAACAACTC AGAGGACGCA
    TCCCAAGACT TTGGGCCACA AGCATTCCAG CTACTGTCTG CTGTGGACAT CCTGCAGGAG
    AAATTTGGAA TTGGGATTCC GATCTTATTT CTCCGAGGAT CTAATTCTCA GCGTCTTCCT
    GATAAATATC GGGGTCACAG GCTCTTTGGT GCTGGAAAGG AGCAAGCAGA AAGTTGGTGG
    AAGACCCTTT CTCACCATCT CATAGCTGAA GGATTCTTGG TAGAAGTTCC CAAGGAAAAC
    AAATATATAA AGACATGTTC CCTCACAAAA AAGGGTAGAA AGTGGCTTGG AGAAGCCAGT
    TCGCAGTCTC CTCCGAGCCT TCTCCTTCAA GCTAATGAAG AGATGTTTCC AAGGAAAGTT
    CTGCTACCAA GTTCTAATCC TGTATCTCCA GAAACGACGC AACATTCCTC TAATCAAAAC
    CCAGCTGGAT TAACTACCAA GCAGTCTAAT TTGGAGAGAA CGCATTCTTA CAAAGTGCCT
    GAGAAAGTTT CTTCTGGGAC TAACATTCCT AAAAAAAGTG CCGTGATGCC GTCACCAGGA
    ACATCTTCCA GCCCCTTAGA ACCTGCCATC TCAGCCCAAG AGCTGGACGC TCGGACTGGG
    CTATATGCCA GGCTGGTGGA AGCAAGGCAG AAACACGCTA ATAAGATGGA TGTACCTCCA
    GCTATTTTAG CAACAAACAA GGTTCTGCTG GACATGGCTA AAATGAGACC GACTACTGTT
    GAAAACATGA AACAGATCGA CGGTGTCTCT GAAGGCAAAG CTGCTCTGTT GGCCCCTCTG
    TTGGAAGTCA TCAAACATTT CTGTCAAGTA ACTAGTGTTC AGACAGACCT CCTTTCCAGT
    GCCAAACCTC ACAAGGAACA GGAGAAAAGT CAGGAGATGG AAAAGAAAGA CTGCTCACTC
    CCCCAGTCTG TGGCCGTCAC ATACACTCTA TTCCAGGAAA AGAAAATGCC CTTACACAGC
    ATAGCTGAGA ACAGGCTCCT GCCTCTCACA GCAGCCGGCA TGCACTTAGC CCAGGCGGTG
    AAAGCCGGCT ACCCCCTGGA TATGGAGCGA GCTGGCCTGA CCCCAGAGAC TTGGAAGATT
    ATTATGGATG TCATCCGAAA CCCTCCCATC AACTCAGATA TGTATAAAGT TAAACTCATC
    AGAATGTTAG TTCCTGAAAA CTTAGACACG TACCTCATCC ACATGGCGAT TGAGATTCTT
    CAGAGTGGTT CCGACAGCAG AACCCAGCCT CCTTGTGATT CCAGCAGGAA GAGGCGTTTC
    CCCAGCTCTG CAGAGAGTTG TGAGAGCTGT AAGGAGAGCA AAGAGGCGGT CACCGAGACC
    AAGGCATCAT CTTCAGAGTC AAAGAGAAAA TTACCCGAGT GGTTTGCCAA AGGAAATGTG
    CCCTCAGCTG ATACCGGCAG CTCATCATCA ATGGCCAAGA CCAAAAAGAA AGGTCTCTTT
    AGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006509154.1
    Translation

    Target ORF information:

    RefSeq Version XM_006509091.2
    Organism Mus musculus(House mouse)
    Definition Mus musculus Werner syndrome homolog (human) (Wrn), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006509091.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGAAACCA CTTCACTACA GCGGAAATTT CCAGAATGGA TGTCTATGCA GAGTCAAAGA 
    TGTGCTACAG AAGAAAAGGC CTGCGTTCAG AAGAGTGTTC TTGAAGATAA CCTCCCATTC
    TTAGAATTCC CTGGATCCAT TGTTTACAGT TATGAAGCTA GTGATTGCTC CTTCCTGTCT
    GAAGACATTA GCATGCGTCT GTCTGATGGC GATGTGGTGG GATTTGACAT GGAATGGCCG
    CCCATATACA AGCCAGGGAA AAGAAGCAGA GTCGCAGTGA TCCAGTTGTG TGTGTCTGAG
    AGCAAATGTT ACTTGTTTCA CATTTCTTCC ATGTCAGTTT TCCCCCAGGG ATTAAAAATG
    TTACTAGAAA ACAAATCAAT TAAGAAGGCA GGGGTTGGGA TTGAAGGGGA CCAGTGGAAA
    CTTCTGCGTG ATTTTGACGT CAAGTTGGAG AGTTTTGTGG AGCTGACGGA TGTTGCCAAT
    GAAAAGTTGA AGTGCGCAGA GACCTGGAGC CTCAATGGTC TGGTTAAACA CGTCTTAGGG
    AAACAACTTT TGAAAGACAA GTCCATCCGC TGCAGCAATT GGAGTAATTT CCCCCTCACT
    GAGGACCAGA AACTGTATGC AGCCACTGAT GCTTATGCTG GTCTTATCAT CTATCAAAAA
    TTAGGAAATT TGGGTGATAC TGCGCAAGTG TTTGCTCTAA ATAAAGCAGA GGAAAACCTA
    CCTCTGGAGA TGAAGAAACA GTTGAATTCA ATCTCCGAAG AAATGAGGGA CCTAGCCAAT
    CGTTTTCCTG TCACTTGCAG AAATTTGGAA ACTCTCCAGA GGGTTCCTGT AATATTGAAG
    AGTATTTCAG AAAATCTCTG TTCATTGAGA AAAGTGATCT GTGGTCCTAC AAACACTGAG
    ACTAGACTGA AGCCGGGCAG TAGTTTTAAT TTACTGTCAT CAGAGGATTC AGCTGCTGCT
    GGAGAAAAAG AGAAACAGAT TGGAAAACAT AGTACTTTTG CTAAAATTAA AGAAGAACCA
    TGGGACCCAG AACTTGACAG TTTAGTGAAG CAAGAGGAGG TTGATGTATT TAGAAATCAA
    GTGAAGCAAG AAAAAGGTGA ATCTGAAAAT GAAATAGAAG ACAATCTGTT GAGAGAAGAT
    ATGGAAAGAA CTTGTGTGAT TCCTAGTATT TCAGAAAATG AACTCCAAGA TTTGGAACAG
    CAAGCTAAAG AAGAAAAATA TAATGATGTT TCTCACCAAC TTTCTGAGCA TTTATCTCCC
    AATGATGATG AGAATGACTC CTCCTATATA ATTGAAAGTG ATGAAGATTT GGAAATGGAG
    ATGCTGAAGT CTTTAGAAAA CCTAAATAGT GACGTGGTGG AACCCACTCA CTCTACATGG
    TTGGAAATGG GAACCAATGG GCGTCTTCCT CCTGAGGAGG AAGATGGACA CGGAAATGAA
    GCCATCAAAG AGGAGCAGGA AGAAGAGGAC CATTTATTGC CGGAACCCAA CGCAAAGCAA
    ATTAATTGCC TCAAGACCTA TTTCGGACAC AGCAGTTTTA AACCGGTTCA GTGGAAAGTC
    ATCCATTCTG TATTAGAAGA GAGAAGAGAT AATGTTGTTG TCATGGCAAC TGGATATGGG
    AAGAGTCTGT GCTTCCAGTA TCCGCCTGTT TATACAGGCA AGATTGGCAT TGTCATTTCA
    CCTCTCATTT CCTTAATGGA AGACCAAGTC CTCCAGCTTG AGCTGTCCAA TGTTCCAGCC
    TGTTTACTTG GATCTGCACA GTCAAAAAAT ATTCTAGGAG ATGTTAAATT AGGCAAATAT
    AGGGTCATCT ACATAACTCC AGAGTTCTGT TCTGGTAACT TGGATCTACT CCAGCAACTT
    GACTCTAGTA TTGGCATCAC TCTCATTGCT GTGGATGAGG CTCACTGCAT TTCAGAGTGG
    GGCCATGATT TCAGAAGTTC ATTCAGGATG CTGGGCTCTC TTAAAACAGC GCTCCCATTG
    GTTCCAGTCA TTGCACTCTC CGCTACTGCA AGCTCTTCCA TCCGGGAAGA CATTATAAGC
    TGCTTAAACC TGAAAGACCC TCAGATCACC TGCACTGGAT TTGATCGGCC AAATCTGTAC
    TTAGAAGTTG GACGGAAAAC AGGGAACATC CTTCAGGATC TAAAGCCGTT TCTCGTCCGA
    AAGGCAAGTT CTGCCTGGGA ATTTGAAGGT CCAACCATCA TCTATTGTCC TTCGAGAAAA
    ATGACAGAAC AAGTTACTGC TGAACTTGGG AAACTGAACT TAGCCTGCAG AACATACCAC
    GCTGGCATGA AAATTAGCGA AAGGAAGGAC GTTCATCATA GGTTCCTGAG AGATGAAATT
    CAGTGTGTTG TAGCTACTGT AGCTTTTGGA ATGGGCATTA ATAAAGCTGA CATTCGCAAA
    GTTATTCATT ATGGTGCGCC TAAGGAAATG GAATCCTATT ACCAGGAAAT TGGTAGAGCT
    GGCCGGGATG GACTTCAGAG TTCCTGTCAC TTGCTCTGGG CTCCAGCAGA CTTTAACACA
    TCCAGGAATC TCCTTATTGA GATTCACGAT GAAAAGTTCC GGTTATATAA ATTAAAGATG
    ATGGTAAAGA TGGAAAAATA CCTTCACTCC AGTCAGTGTA GGCGACGAAT CATCTTGTCC
    CATTTTGAGG ACAAATGTCT GCAGAAGGCC TCCTTGGACA TTATGGGAAC TGAAAAATGC
    TGTGATAATT GCAGGCCCAG GCTGAATCAT TGCCTTACTG CTAACAACTC AGAGGACGCA
    TCCCAAGACT TTGGGCCACA AGCATTCCAG CTACTGTCTG CTGTGGACAT CCTGCAGGAG
    AAATTTGGAA TTGGGATTCC GATCTTATTT CTCCGAGGAT CTAATTCTCA GCGTCTTCCT
    GATAAATATC GGGGTCACAG GCTCTTTGGT GCTGGAAAGG AGCAAGCAGA AAGTTGGTGG
    AAGACCCTTT CTCACCATCT CATAGCTGAA GGATTCTTGG TAGAAGTTCC CAAGGAAAAC
    AAATATATAA AGACATGTTC CCTCACAAAA AAGGGTAGAA AGTGGCTTGG AGAAGCCAGT
    TCGCAGTCTC CTCCGAGCCT TCTCCTTCAA GCTAATGAAG AGATGTTTCC AAGGAAAGTT
    CTGCTACCAA GTTCTAATCC TGTATCTCCA GAAACGACGC AACATTCCTC TAATCAAAAC
    CCAGCTGGAT TAACTACCAA GCAGTCTAAT TTGGAGAGAA CGCATTCTTA CAAAGTGCCT
    GAGAAAGTTT CTTCTGGGAC TAACATTCCT AAAAAAAGTG CCGTGATGCC GTCACCAGGA
    ACATCTTCCA GCCCCTTAGA ACCTGCCATC TCAGCCCAAG AGCTGGACGC TCGGACTGGG
    CTATATGCCA GGCTGGTGGA AGCAAGGCAG AAACACGCTA ATAAGATGGA TGTACCTCCA
    GCTATTTTAG CAACAAACAA GGTTCTGCTG GACATGGCTA AAATGAGACC GACTACTGTT
    GAAAACATGA AACAGATCGA CGGTGTCTCT GAAGGCAAAG CTGCTCTGTT GGCCCCTCTG
    TTGGAAGTCA TCAAACATTT CTGTCAAGTA ACTAGTGTTC AGACAGACCT CCTTTCCAGT
    GCCAAACCTC ACAAGGAACA GGAGAAAAGT CAGGAGATGG AAAAGAAAGA CTGCTCACTC
    CCCCAGTCTG TGGCCGTCAC ATACACTCTA TTCCAGGAAA AGAAAATGCC CTTACACAGC
    ATAGCTGAGA ACAGGCTCCT GCCTCTCACA GCAGCCGGCA TGCACTTAGC CCAGGCGGTG
    AAAGCCGGCT ACCCCCTGGA TATGGAGCGA GCTGGCCTGA CCCCAGAGAC TTGGAAGATT
    ATTATGGATG TCATCCGAAA CCCTCCCATC AACTCAGATA TGTATAAAGT TAAACTCATC
    AGAATGTTAG TTCCTGAAAA CTTAGACACG TACCTCATCC ACATGGCGAT TGAGATTCTT
    CAGAGTGGTT CCGACAGCAG AACCCAGCCT CCTTGTGATT CCAGCAGGAA GAGGCGTTTC
    CCCAGCTCTG CAGAGAGTTG TGAGAGCTGT AAGGAGAGCA AAGAGGCGGT CACCGAGACC
    AAGGCATCAT CTTCAGAGTC AAAGAGAAAA TTACCCGAGT GGTTTGCCAA AGGAAATGTG
    CCCTCAGCTG ATACCGGCAG CTCATCATCA ATGGCCAAGA CCAAAAAGAA AGGTCTCTTT
    AGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu21413
    Clone ID Related Accession (Same CDS sequence) NM_011721.4 , XM_006509091.2 , XM_006509091.3 , XM_017312662.1 , XM_006509091.4 , XM_017312662.2 , NM_001122822.1
    Accession Version XM_006509091.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4206bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product Werner syndrome ATP-dependent helicase homolog isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006509091.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGAAACCA CTTCACTACA GCGGAAATTT CCAGAATGGA TGTCTATGCA GAGTCAAAGA 
    TGTGCTACAG AAGAAAAGGC CTGCGTTCAG AAGAGTGTTC TTGAAGATAA CCTCCCATTC
    TTAGAATTCC CTGGATCCAT TGTTTACAGT TATGAAGCTA GTGATTGCTC CTTCCTGTCT
    GAAGACATTA GCATGCGTCT GTCTGATGGC GATGTGGTGG GATTTGACAT GGAATGGCCG
    CCCATATACA AGCCAGGGAA AAGAAGCAGA GTCGCAGTGA TCCAGTTGTG TGTGTCTGAG
    AGCAAATGTT ACTTGTTTCA CATTTCTTCC ATGTCAGTTT TCCCCCAGGG ATTAAAAATG
    TTACTAGAAA ACAAATCAAT TAAGAAGGCA GGGGTTGGGA TTGAAGGGGA CCAGTGGAAA
    CTTCTGCGTG ATTTTGACGT CAAGTTGGAG AGTTTTGTGG AGCTGACGGA TGTTGCCAAT
    GAAAAGTTGA AGTGCGCAGA GACCTGGAGC CTCAATGGTC TGGTTAAACA CGTCTTAGGG
    AAACAACTTT TGAAAGACAA GTCCATCCGC TGCAGCAATT GGAGTAATTT CCCCCTCACT
    GAGGACCAGA AACTGTATGC AGCCACTGAT GCTTATGCTG GTCTTATCAT CTATCAAAAA
    TTAGGAAATT TGGGTGATAC TGCGCAAGTG TTTGCTCTAA ATAAAGCAGA GGAAAACCTA
    CCTCTGGAGA TGAAGAAACA GTTGAATTCA ATCTCCGAAG AAATGAGGGA CCTAGCCAAT
    CGTTTTCCTG TCACTTGCAG AAATTTGGAA ACTCTCCAGA GGGTTCCTGT AATATTGAAG
    AGTATTTCAG AAAATCTCTG TTCATTGAGA AAAGTGATCT GTGGTCCTAC AAACACTGAG
    ACTAGACTGA AGCCGGGCAG TAGTTTTAAT TTACTGTCAT CAGAGGATTC AGCTGCTGCT
    GGAGAAAAAG AGAAACAGAT TGGAAAACAT AGTACTTTTG CTAAAATTAA AGAAGAACCA
    TGGGACCCAG AACTTGACAG TTTAGTGAAG CAAGAGGAGG TTGATGTATT TAGAAATCAA
    GTGAAGCAAG AAAAAGGTGA ATCTGAAAAT GAAATAGAAG ACAATCTGTT GAGAGAAGAT
    ATGGAAAGAA CTTGTGTGAT TCCTAGTATT TCAGAAAATG AACTCCAAGA TTTGGAACAG
    CAAGCTAAAG AAGAAAAATA TAATGATGTT TCTCACCAAC TTTCTGAGCA TTTATCTCCC
    AATGATGATG AGAATGACTC CTCCTATATA ATTGAAAGTG ATGAAGATTT GGAAATGGAG
    ATGCTGAAGT CTTTAGAAAA CCTAAATAGT GACGTGGTGG AACCCACTCA CTCTACATGG
    TTGGAAATGG GAACCAATGG GCGTCTTCCT CCTGAGGAGG AAGATGGACA CGGAAATGAA
    GCCATCAAAG AGGAGCAGGA AGAAGAGGAC CATTTATTGC CGGAACCCAA CGCAAAGCAA
    ATTAATTGCC TCAAGACCTA TTTCGGACAC AGCAGTTTTA AACCGGTTCA GTGGAAAGTC
    ATCCATTCTG TATTAGAAGA GAGAAGAGAT AATGTTGTTG TCATGGCAAC TGGATATGGG
    AAGAGTCTGT GCTTCCAGTA TCCGCCTGTT TATACAGGCA AGATTGGCAT TGTCATTTCA
    CCTCTCATTT CCTTAATGGA AGACCAAGTC CTCCAGCTTG AGCTGTCCAA TGTTCCAGCC
    TGTTTACTTG GATCTGCACA GTCAAAAAAT ATTCTAGGAG ATGTTAAATT AGGCAAATAT
    AGGGTCATCT ACATAACTCC AGAGTTCTGT TCTGGTAACT TGGATCTACT CCAGCAACTT
    GACTCTAGTA TTGGCATCAC TCTCATTGCT GTGGATGAGG CTCACTGCAT TTCAGAGTGG
    GGCCATGATT TCAGAAGTTC ATTCAGGATG CTGGGCTCTC TTAAAACAGC GCTCCCATTG
    GTTCCAGTCA TTGCACTCTC CGCTACTGCA AGCTCTTCCA TCCGGGAAGA CATTATAAGC
    TGCTTAAACC TGAAAGACCC TCAGATCACC TGCACTGGAT TTGATCGGCC AAATCTGTAC
    TTAGAAGTTG GACGGAAAAC AGGGAACATC CTTCAGGATC TAAAGCCGTT TCTCGTCCGA
    AAGGCAAGTT CTGCCTGGGA ATTTGAAGGT CCAACCATCA TCTATTGTCC TTCGAGAAAA
    ATGACAGAAC AAGTTACTGC TGAACTTGGG AAACTGAACT TAGCCTGCAG AACATACCAC
    GCTGGCATGA AAATTAGCGA AAGGAAGGAC GTTCATCATA GGTTCCTGAG AGATGAAATT
    CAGTGTGTTG TAGCTACTGT AGCTTTTGGA ATGGGCATTA ATAAAGCTGA CATTCGCAAA
    GTTATTCATT ATGGTGCGCC TAAGGAAATG GAATCCTATT ACCAGGAAAT TGGTAGAGCT
    GGCCGGGATG GACTTCAGAG TTCCTGTCAC TTGCTCTGGG CTCCAGCAGA CTTTAACACA
    TCCAGGAATC TCCTTATTGA GATTCACGAT GAAAAGTTCC GGTTATATAA ATTAAAGATG
    ATGGTAAAGA TGGAAAAATA CCTTCACTCC AGTCAGTGTA GGCGACGAAT CATCTTGTCC
    CATTTTGAGG ACAAATGTCT GCAGAAGGCC TCCTTGGACA TTATGGGAAC TGAAAAATGC
    TGTGATAATT GCAGGCCCAG GCTGAATCAT TGCCTTACTG CTAACAACTC AGAGGACGCA
    TCCCAAGACT TTGGGCCACA AGCATTCCAG CTACTGTCTG CTGTGGACAT CCTGCAGGAG
    AAATTTGGAA TTGGGATTCC GATCTTATTT CTCCGAGGAT CTAATTCTCA GCGTCTTCCT
    GATAAATATC GGGGTCACAG GCTCTTTGGT GCTGGAAAGG AGCAAGCAGA AAGTTGGTGG
    AAGACCCTTT CTCACCATCT CATAGCTGAA GGATTCTTGG TAGAAGTTCC CAAGGAAAAC
    AAATATATAA AGACATGTTC CCTCACAAAA AAGGGTAGAA AGTGGCTTGG AGAAGCCAGT
    TCGCAGTCTC CTCCGAGCCT TCTCCTTCAA GCTAATGAAG AGATGTTTCC AAGGAAAGTT
    CTGCTACCAA GTTCTAATCC TGTATCTCCA GAAACGACGC AACATTCCTC TAATCAAAAC
    CCAGCTGGAT TAACTACCAA GCAGTCTAAT TTGGAGAGAA CGCATTCTTA CAAAGTGCCT
    GAGAAAGTTT CTTCTGGGAC TAACATTCCT AAAAAAAGTG CCGTGATGCC GTCACCAGGA
    ACATCTTCCA GCCCCTTAGA ACCTGCCATC TCAGCCCAAG AGCTGGACGC TCGGACTGGG
    CTATATGCCA GGCTGGTGGA AGCAAGGCAG AAACACGCTA ATAAGATGGA TGTACCTCCA
    GCTATTTTAG CAACAAACAA GGTTCTGCTG GACATGGCTA AAATGAGACC GACTACTGTT
    GAAAACATGA AACAGATCGA CGGTGTCTCT GAAGGCAAAG CTGCTCTGTT GGCCCCTCTG
    TTGGAAGTCA TCAAACATTT CTGTCAAGTA ACTAGTGTTC AGACAGACCT CCTTTCCAGT
    GCCAAACCTC ACAAGGAACA GGAGAAAAGT CAGGAGATGG AAAAGAAAGA CTGCTCACTC
    CCCCAGTCTG TGGCCGTCAC ATACACTCTA TTCCAGGAAA AGAAAATGCC CTTACACAGC
    ATAGCTGAGA ACAGGCTCCT GCCTCTCACA GCAGCCGGCA TGCACTTAGC CCAGGCGGTG
    AAAGCCGGCT ACCCCCTGGA TATGGAGCGA GCTGGCCTGA CCCCAGAGAC TTGGAAGATT
    ATTATGGATG TCATCCGAAA CCCTCCCATC AACTCAGATA TGTATAAAGT TAAACTCATC
    AGAATGTTAG TTCCTGAAAA CTTAGACACG TACCTCATCC ACATGGCGAT TGAGATTCTT
    CAGAGTGGTT CCGACAGCAG AACCCAGCCT CCTTGTGATT CCAGCAGGAA GAGGCGTTTC
    CCCAGCTCTG CAGAGAGTTG TGAGAGCTGT AAGGAGAGCA AAGAGGCGGT CACCGAGACC
    AAGGCATCAT CTTCAGAGTC AAAGAGAAAA TTACCCGAGT GGTTTGCCAA AGGAAATGTG
    CCCTCAGCTG ATACCGGCAG CTCATCATCA ATGGCCAAGA CCAAAAAGAA AGGTCTCTTT
    AGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006509154.1
    CDS330..4535
    Translation

    Target ORF information:

    RefSeq Version XM_006509091.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus Werner syndrome homolog (human) (Wrn), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006509091.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGAAACCA CTTCACTACA GCGGAAATTT CCAGAATGGA TGTCTATGCA GAGTCAAAGA 
    TGTGCTACAG AAGAAAAGGC CTGCGTTCAG AAGAGTGTTC TTGAAGATAA CCTCCCATTC
    TTAGAATTCC CTGGATCCAT TGTTTACAGT TATGAAGCTA GTGATTGCTC CTTCCTGTCT
    GAAGACATTA GCATGCGTCT GTCTGATGGC GATGTGGTGG GATTTGACAT GGAATGGCCG
    CCCATATACA AGCCAGGGAA AAGAAGCAGA GTCGCAGTGA TCCAGTTGTG TGTGTCTGAG
    AGCAAATGTT ACTTGTTTCA CATTTCTTCC ATGTCAGTTT TCCCCCAGGG ATTAAAAATG
    TTACTAGAAA ACAAATCAAT TAAGAAGGCA GGGGTTGGGA TTGAAGGGGA CCAGTGGAAA
    CTTCTGCGTG ATTTTGACGT CAAGTTGGAG AGTTTTGTGG AGCTGACGGA TGTTGCCAAT
    GAAAAGTTGA AGTGCGCAGA GACCTGGAGC CTCAATGGTC TGGTTAAACA CGTCTTAGGG
    AAACAACTTT TGAAAGACAA GTCCATCCGC TGCAGCAATT GGAGTAATTT CCCCCTCACT
    GAGGACCAGA AACTGTATGC AGCCACTGAT GCTTATGCTG GTCTTATCAT CTATCAAAAA
    TTAGGAAATT TGGGTGATAC TGCGCAAGTG TTTGCTCTAA ATAAAGCAGA GGAAAACCTA
    CCTCTGGAGA TGAAGAAACA GTTGAATTCA ATCTCCGAAG AAATGAGGGA CCTAGCCAAT
    CGTTTTCCTG TCACTTGCAG AAATTTGGAA ACTCTCCAGA GGGTTCCTGT AATATTGAAG
    AGTATTTCAG AAAATCTCTG TTCATTGAGA AAAGTGATCT GTGGTCCTAC AAACACTGAG
    ACTAGACTGA AGCCGGGCAG TAGTTTTAAT TTACTGTCAT CAGAGGATTC AGCTGCTGCT
    GGAGAAAAAG AGAAACAGAT TGGAAAACAT AGTACTTTTG CTAAAATTAA AGAAGAACCA
    TGGGACCCAG AACTTGACAG TTTAGTGAAG CAAGAGGAGG TTGATGTATT TAGAAATCAA
    GTGAAGCAAG AAAAAGGTGA ATCTGAAAAT GAAATAGAAG ACAATCTGTT GAGAGAAGAT
    ATGGAAAGAA CTTGTGTGAT TCCTAGTATT TCAGAAAATG AACTCCAAGA TTTGGAACAG
    CAAGCTAAAG AAGAAAAATA TAATGATGTT TCTCACCAAC TTTCTGAGCA TTTATCTCCC
    AATGATGATG AGAATGACTC CTCCTATATA ATTGAAAGTG ATGAAGATTT GGAAATGGAG
    ATGCTGAAGT CTTTAGAAAA CCTAAATAGT GACGTGGTGG AACCCACTCA CTCTACATGG
    TTGGAAATGG GAACCAATGG GCGTCTTCCT CCTGAGGAGG AAGATGGACA CGGAAATGAA
    GCCATCAAAG AGGAGCAGGA AGAAGAGGAC CATTTATTGC CGGAACCCAA CGCAAAGCAA
    ATTAATTGCC TCAAGACCTA TTTCGGACAC AGCAGTTTTA AACCGGTTCA GTGGAAAGTC
    ATCCATTCTG TATTAGAAGA GAGAAGAGAT AATGTTGTTG TCATGGCAAC TGGATATGGG
    AAGAGTCTGT GCTTCCAGTA TCCGCCTGTT TATACAGGCA AGATTGGCAT TGTCATTTCA
    CCTCTCATTT CCTTAATGGA AGACCAAGTC CTCCAGCTTG AGCTGTCCAA TGTTCCAGCC
    TGTTTACTTG GATCTGCACA GTCAAAAAAT ATTCTAGGAG ATGTTAAATT AGGCAAATAT
    AGGGTCATCT ACATAACTCC AGAGTTCTGT TCTGGTAACT TGGATCTACT CCAGCAACTT
    GACTCTAGTA TTGGCATCAC TCTCATTGCT GTGGATGAGG CTCACTGCAT TTCAGAGTGG
    GGCCATGATT TCAGAAGTTC ATTCAGGATG CTGGGCTCTC TTAAAACAGC GCTCCCATTG
    GTTCCAGTCA TTGCACTCTC CGCTACTGCA AGCTCTTCCA TCCGGGAAGA CATTATAAGC
    TGCTTAAACC TGAAAGACCC TCAGATCACC TGCACTGGAT TTGATCGGCC AAATCTGTAC
    TTAGAAGTTG GACGGAAAAC AGGGAACATC CTTCAGGATC TAAAGCCGTT TCTCGTCCGA
    AAGGCAAGTT CTGCCTGGGA ATTTGAAGGT CCAACCATCA TCTATTGTCC TTCGAGAAAA
    ATGACAGAAC AAGTTACTGC TGAACTTGGG AAACTGAACT TAGCCTGCAG AACATACCAC
    GCTGGCATGA AAATTAGCGA AAGGAAGGAC GTTCATCATA GGTTCCTGAG AGATGAAATT
    CAGTGTGTTG TAGCTACTGT AGCTTTTGGA ATGGGCATTA ATAAAGCTGA CATTCGCAAA
    GTTATTCATT ATGGTGCGCC TAAGGAAATG GAATCCTATT ACCAGGAAAT TGGTAGAGCT
    GGCCGGGATG GACTTCAGAG TTCCTGTCAC TTGCTCTGGG CTCCAGCAGA CTTTAACACA
    TCCAGGAATC TCCTTATTGA GATTCACGAT GAAAAGTTCC GGTTATATAA ATTAAAGATG
    ATGGTAAAGA TGGAAAAATA CCTTCACTCC AGTCAGTGTA GGCGACGAAT CATCTTGTCC
    CATTTTGAGG ACAAATGTCT GCAGAAGGCC TCCTTGGACA TTATGGGAAC TGAAAAATGC
    TGTGATAATT GCAGGCCCAG GCTGAATCAT TGCCTTACTG CTAACAACTC AGAGGACGCA
    TCCCAAGACT TTGGGCCACA AGCATTCCAG CTACTGTCTG CTGTGGACAT CCTGCAGGAG
    AAATTTGGAA TTGGGATTCC GATCTTATTT CTCCGAGGAT CTAATTCTCA GCGTCTTCCT
    GATAAATATC GGGGTCACAG GCTCTTTGGT GCTGGAAAGG AGCAAGCAGA AAGTTGGTGG
    AAGACCCTTT CTCACCATCT CATAGCTGAA GGATTCTTGG TAGAAGTTCC CAAGGAAAAC
    AAATATATAA AGACATGTTC CCTCACAAAA AAGGGTAGAA AGTGGCTTGG AGAAGCCAGT
    TCGCAGTCTC CTCCGAGCCT TCTCCTTCAA GCTAATGAAG AGATGTTTCC AAGGAAAGTT
    CTGCTACCAA GTTCTAATCC TGTATCTCCA GAAACGACGC AACATTCCTC TAATCAAAAC
    CCAGCTGGAT TAACTACCAA GCAGTCTAAT TTGGAGAGAA CGCATTCTTA CAAAGTGCCT
    GAGAAAGTTT CTTCTGGGAC TAACATTCCT AAAAAAAGTG CCGTGATGCC GTCACCAGGA
    ACATCTTCCA GCCCCTTAGA ACCTGCCATC TCAGCCCAAG AGCTGGACGC TCGGACTGGG
    CTATATGCCA GGCTGGTGGA AGCAAGGCAG AAACACGCTA ATAAGATGGA TGTACCTCCA
    GCTATTTTAG CAACAAACAA GGTTCTGCTG GACATGGCTA AAATGAGACC GACTACTGTT
    GAAAACATGA AACAGATCGA CGGTGTCTCT GAAGGCAAAG CTGCTCTGTT GGCCCCTCTG
    TTGGAAGTCA TCAAACATTT CTGTCAAGTA ACTAGTGTTC AGACAGACCT CCTTTCCAGT
    GCCAAACCTC ACAAGGAACA GGAGAAAAGT CAGGAGATGG AAAAGAAAGA CTGCTCACTC
    CCCCAGTCTG TGGCCGTCAC ATACACTCTA TTCCAGGAAA AGAAAATGCC CTTACACAGC
    ATAGCTGAGA ACAGGCTCCT GCCTCTCACA GCAGCCGGCA TGCACTTAGC CCAGGCGGTG
    AAAGCCGGCT ACCCCCTGGA TATGGAGCGA GCTGGCCTGA CCCCAGAGAC TTGGAAGATT
    ATTATGGATG TCATCCGAAA CCCTCCCATC AACTCAGATA TGTATAAAGT TAAACTCATC
    AGAATGTTAG TTCCTGAAAA CTTAGACACG TACCTCATCC ACATGGCGAT TGAGATTCTT
    CAGAGTGGTT CCGACAGCAG AACCCAGCCT CCTTGTGATT CCAGCAGGAA GAGGCGTTTC
    CCCAGCTCTG CAGAGAGTTG TGAGAGCTGT AAGGAGAGCA AAGAGGCGGT CACCGAGACC
    AAGGCATCAT CTTCAGAGTC AAAGAGAAAA TTACCCGAGT GGTTTGCCAA AGGAAATGTG
    CCCTCAGCTG ATACCGGCAG CTCATCATCA ATGGCCAAGA CCAAAAAGAA AGGTCTCTTT
    AGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu21413
    Clone ID Related Accession (Same CDS sequence) NM_011721.4 , XM_006509091.2 , XM_006509091.3 , XM_017312662.1 , XM_006509091.4 , XM_017312662.2 , NM_001122822.1
    Accession Version XM_017312662.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4206bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product Werner syndrome ATP-dependent helicase homolog isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGAAACCA CTTCACTACA GCGGAAATTT CCAGAATGGA TGTCTATGCA GAGTCAAAGA 
    TGTGCTACAG AAGAAAAGGC CTGCGTTCAG AAGAGTGTTC TTGAAGATAA CCTCCCATTC
    TTAGAATTCC CTGGATCCAT TGTTTACAGT TATGAAGCTA GTGATTGCTC CTTCCTGTCT
    GAAGACATTA GCATGCGTCT GTCTGATGGC GATGTGGTGG GATTTGACAT GGAATGGCCG
    CCCATATACA AGCCAGGGAA AAGAAGCAGA GTCGCAGTGA TCCAGTTGTG TGTGTCTGAG
    AGCAAATGTT ACTTGTTTCA CATTTCTTCC ATGTCAGTTT TCCCCCAGGG ATTAAAAATG
    TTACTAGAAA ACAAATCAAT TAAGAAGGCA GGGGTTGGGA TTGAAGGGGA CCAGTGGAAA
    CTTCTGCGTG ATTTTGACGT CAAGTTGGAG AGTTTTGTGG AGCTGACGGA TGTTGCCAAT
    GAAAAGTTGA AGTGCGCAGA GACCTGGAGC CTCAATGGTC TGGTTAAACA CGTCTTAGGG
    AAACAACTTT TGAAAGACAA GTCCATCCGC TGCAGCAATT GGAGTAATTT CCCCCTCACT
    GAGGACCAGA AACTGTATGC AGCCACTGAT GCTTATGCTG GTCTTATCAT CTATCAAAAA
    TTAGGAAATT TGGGTGATAC TGCGCAAGTG TTTGCTCTAA ATAAAGCAGA GGAAAACCTA
    CCTCTGGAGA TGAAGAAACA GTTGAATTCA ATCTCCGAAG AAATGAGGGA CCTAGCCAAT
    CGTTTTCCTG TCACTTGCAG AAATTTGGAA ACTCTCCAGA GGGTTCCTGT AATATTGAAG
    AGTATTTCAG AAAATCTCTG TTCATTGAGA AAAGTGATCT GTGGTCCTAC AAACACTGAG
    ACTAGACTGA AGCCGGGCAG TAGTTTTAAT TTACTGTCAT CAGAGGATTC AGCTGCTGCT
    GGAGAAAAAG AGAAACAGAT TGGAAAACAT AGTACTTTTG CTAAAATTAA AGAAGAACCA
    TGGGACCCAG AACTTGACAG TTTAGTGAAG CAAGAGGAGG TTGATGTATT TAGAAATCAA
    GTGAAGCAAG AAAAAGGTGA ATCTGAAAAT GAAATAGAAG ACAATCTGTT GAGAGAAGAT
    ATGGAAAGAA CTTGTGTGAT TCCTAGTATT TCAGAAAATG AACTCCAAGA TTTGGAACAG
    CAAGCTAAAG AAGAAAAATA TAATGATGTT TCTCACCAAC TTTCTGAGCA TTTATCTCCC
    AATGATGATG AGAATGACTC CTCCTATATA ATTGAAAGTG ATGAAGATTT GGAAATGGAG
    ATGCTGAAGT CTTTAGAAAA CCTAAATAGT GACGTGGTGG AACCCACTCA CTCTACATGG
    TTGGAAATGG GAACCAATGG GCGTCTTCCT CCTGAGGAGG AAGATGGACA CGGAAATGAA
    GCCATCAAAG AGGAGCAGGA AGAAGAGGAC CATTTATTGC CGGAACCCAA CGCAAAGCAA
    ATTAATTGCC TCAAGACCTA TTTCGGACAC AGCAGTTTTA AACCGGTTCA GTGGAAAGTC
    ATCCATTCTG TATTAGAAGA GAGAAGAGAT AATGTTGTTG TCATGGCAAC TGGATATGGG
    AAGAGTCTGT GCTTCCAGTA TCCGCCTGTT TATACAGGCA AGATTGGCAT TGTCATTTCA
    CCTCTCATTT CCTTAATGGA AGACCAAGTC CTCCAGCTTG AGCTGTCCAA TGTTCCAGCC
    TGTTTACTTG GATCTGCACA GTCAAAAAAT ATTCTAGGAG ATGTTAAATT AGGCAAATAT
    AGGGTCATCT ACATAACTCC AGAGTTCTGT TCTGGTAACT TGGATCTACT CCAGCAACTT
    GACTCTAGTA TTGGCATCAC TCTCATTGCT GTGGATGAGG CTCACTGCAT TTCAGAGTGG
    GGCCATGATT TCAGAAGTTC ATTCAGGATG CTGGGCTCTC TTAAAACAGC GCTCCCATTG
    GTTCCAGTCA TTGCACTCTC CGCTACTGCA AGCTCTTCCA TCCGGGAAGA CATTATAAGC
    TGCTTAAACC TGAAAGACCC TCAGATCACC TGCACTGGAT TTGATCGGCC AAATCTGTAC
    TTAGAAGTTG GACGGAAAAC AGGGAACATC CTTCAGGATC TAAAGCCGTT TCTCGTCCGA
    AAGGCAAGTT CTGCCTGGGA ATTTGAAGGT CCAACCATCA TCTATTGTCC TTCGAGAAAA
    ATGACAGAAC AAGTTACTGC TGAACTTGGG AAACTGAACT TAGCCTGCAG AACATACCAC
    GCTGGCATGA AAATTAGCGA AAGGAAGGAC GTTCATCATA GGTTCCTGAG AGATGAAATT
    CAGTGTGTTG TAGCTACTGT AGCTTTTGGA ATGGGCATTA ATAAAGCTGA CATTCGCAAA
    GTTATTCATT ATGGTGCGCC TAAGGAAATG GAATCCTATT ACCAGGAAAT TGGTAGAGCT
    GGCCGGGATG GACTTCAGAG TTCCTGTCAC TTGCTCTGGG CTCCAGCAGA CTTTAACACA
    TCCAGGAATC TCCTTATTGA GATTCACGAT GAAAAGTTCC GGTTATATAA ATTAAAGATG
    ATGGTAAAGA TGGAAAAATA CCTTCACTCC AGTCAGTGTA GGCGACGAAT CATCTTGTCC
    CATTTTGAGG ACAAATGTCT GCAGAAGGCC TCCTTGGACA TTATGGGAAC TGAAAAATGC
    TGTGATAATT GCAGGCCCAG GCTGAATCAT TGCCTTACTG CTAACAACTC AGAGGACGCA
    TCCCAAGACT TTGGGCCACA AGCATTCCAG CTACTGTCTG CTGTGGACAT CCTGCAGGAG
    AAATTTGGAA TTGGGATTCC GATCTTATTT CTCCGAGGAT CTAATTCTCA GCGTCTTCCT
    GATAAATATC GGGGTCACAG GCTCTTTGGT GCTGGAAAGG AGCAAGCAGA AAGTTGGTGG
    AAGACCCTTT CTCACCATCT CATAGCTGAA GGATTCTTGG TAGAAGTTCC CAAGGAAAAC
    AAATATATAA AGACATGTTC CCTCACAAAA AAGGGTAGAA AGTGGCTTGG AGAAGCCAGT
    TCGCAGTCTC CTCCGAGCCT TCTCCTTCAA GCTAATGAAG AGATGTTTCC AAGGAAAGTT
    CTGCTACCAA GTTCTAATCC TGTATCTCCA GAAACGACGC AACATTCCTC TAATCAAAAC
    CCAGCTGGAT TAACTACCAA GCAGTCTAAT TTGGAGAGAA CGCATTCTTA CAAAGTGCCT
    GAGAAAGTTT CTTCTGGGAC TAACATTCCT AAAAAAAGTG CCGTGATGCC GTCACCAGGA
    ACATCTTCCA GCCCCTTAGA ACCTGCCATC TCAGCCCAAG AGCTGGACGC TCGGACTGGG
    CTATATGCCA GGCTGGTGGA AGCAAGGCAG AAACACGCTA ATAAGATGGA TGTACCTCCA
    GCTATTTTAG CAACAAACAA GGTTCTGCTG GACATGGCTA AAATGAGACC GACTACTGTT
    GAAAACATGA AACAGATCGA CGGTGTCTCT GAAGGCAAAG CTGCTCTGTT GGCCCCTCTG
    TTGGAAGTCA TCAAACATTT CTGTCAAGTA ACTAGTGTTC AGACAGACCT CCTTTCCAGT
    GCCAAACCTC ACAAGGAACA GGAGAAAAGT CAGGAGATGG AAAAGAAAGA CTGCTCACTC
    CCCCAGTCTG TGGCCGTCAC ATACACTCTA TTCCAGGAAA AGAAAATGCC CTTACACAGC
    ATAGCTGAGA ACAGGCTCCT GCCTCTCACA GCAGCCGGCA TGCACTTAGC CCAGGCGGTG
    AAAGCCGGCT ACCCCCTGGA TATGGAGCGA GCTGGCCTGA CCCCAGAGAC TTGGAAGATT
    ATTATGGATG TCATCCGAAA CCCTCCCATC AACTCAGATA TGTATAAAGT TAAACTCATC
    AGAATGTTAG TTCCTGAAAA CTTAGACACG TACCTCATCC ACATGGCGAT TGAGATTCTT
    CAGAGTGGTT CCGACAGCAG AACCCAGCCT CCTTGTGATT CCAGCAGGAA GAGGCGTTTC
    CCCAGCTCTG CAGAGAGTTG TGAGAGCTGT AAGGAGAGCA AAGAGGCGGT CACCGAGACC
    AAGGCATCAT CTTCAGAGTC AAAGAGAAAA TTACCCGAGT GGTTTGCCAA AGGAAATGTG
    CCCTCAGCTG ATACCGGCAG CTCATCATCA ATGGCCAAGA CCAAAAAGAA AGGTCTCTTT
    AGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017168151.1
    CDS382..4587
    Translation

    Target ORF information:

    RefSeq Version XM_017312662.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus Werner syndrome homolog (human) (Wrn), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017312662.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGAAACCA CTTCACTACA GCGGAAATTT CCAGAATGGA TGTCTATGCA GAGTCAAAGA 
    TGTGCTACAG AAGAAAAGGC CTGCGTTCAG AAGAGTGTTC TTGAAGATAA CCTCCCATTC
    TTAGAATTCC CTGGATCCAT TGTTTACAGT TATGAAGCTA GTGATTGCTC CTTCCTGTCT
    GAAGACATTA GCATGCGTCT GTCTGATGGC GATGTGGTGG GATTTGACAT GGAATGGCCG
    CCCATATACA AGCCAGGGAA AAGAAGCAGA GTCGCAGTGA TCCAGTTGTG TGTGTCTGAG
    AGCAAATGTT ACTTGTTTCA CATTTCTTCC ATGTCAGTTT TCCCCCAGGG ATTAAAAATG
    TTACTAGAAA ACAAATCAAT TAAGAAGGCA GGGGTTGGGA TTGAAGGGGA CCAGTGGAAA
    CTTCTGCGTG ATTTTGACGT CAAGTTGGAG AGTTTTGTGG AGCTGACGGA TGTTGCCAAT
    GAAAAGTTGA AGTGCGCAGA GACCTGGAGC CTCAATGGTC TGGTTAAACA CGTCTTAGGG
    AAACAACTTT TGAAAGACAA GTCCATCCGC TGCAGCAATT GGAGTAATTT CCCCCTCACT
    GAGGACCAGA AACTGTATGC AGCCACTGAT GCTTATGCTG GTCTTATCAT CTATCAAAAA
    TTAGGAAATT TGGGTGATAC TGCGCAAGTG TTTGCTCTAA ATAAAGCAGA GGAAAACCTA
    CCTCTGGAGA TGAAGAAACA GTTGAATTCA ATCTCCGAAG AAATGAGGGA CCTAGCCAAT
    CGTTTTCCTG TCACTTGCAG AAATTTGGAA ACTCTCCAGA GGGTTCCTGT AATATTGAAG
    AGTATTTCAG AAAATCTCTG TTCATTGAGA AAAGTGATCT GTGGTCCTAC AAACACTGAG
    ACTAGACTGA AGCCGGGCAG TAGTTTTAAT TTACTGTCAT CAGAGGATTC AGCTGCTGCT
    GGAGAAAAAG AGAAACAGAT TGGAAAACAT AGTACTTTTG CTAAAATTAA AGAAGAACCA
    TGGGACCCAG AACTTGACAG TTTAGTGAAG CAAGAGGAGG TTGATGTATT TAGAAATCAA
    GTGAAGCAAG AAAAAGGTGA ATCTGAAAAT GAAATAGAAG ACAATCTGTT GAGAGAAGAT
    ATGGAAAGAA CTTGTGTGAT TCCTAGTATT TCAGAAAATG AACTCCAAGA TTTGGAACAG
    CAAGCTAAAG AAGAAAAATA TAATGATGTT TCTCACCAAC TTTCTGAGCA TTTATCTCCC
    AATGATGATG AGAATGACTC CTCCTATATA ATTGAAAGTG ATGAAGATTT GGAAATGGAG
    ATGCTGAAGT CTTTAGAAAA CCTAAATAGT GACGTGGTGG AACCCACTCA CTCTACATGG
    TTGGAAATGG GAACCAATGG GCGTCTTCCT CCTGAGGAGG AAGATGGACA CGGAAATGAA
    GCCATCAAAG AGGAGCAGGA AGAAGAGGAC CATTTATTGC CGGAACCCAA CGCAAAGCAA
    ATTAATTGCC TCAAGACCTA TTTCGGACAC AGCAGTTTTA AACCGGTTCA GTGGAAAGTC
    ATCCATTCTG TATTAGAAGA GAGAAGAGAT AATGTTGTTG TCATGGCAAC TGGATATGGG
    AAGAGTCTGT GCTTCCAGTA TCCGCCTGTT TATACAGGCA AGATTGGCAT TGTCATTTCA
    CCTCTCATTT CCTTAATGGA AGACCAAGTC CTCCAGCTTG AGCTGTCCAA TGTTCCAGCC
    TGTTTACTTG GATCTGCACA GTCAAAAAAT ATTCTAGGAG ATGTTAAATT AGGCAAATAT
    AGGGTCATCT ACATAACTCC AGAGTTCTGT TCTGGTAACT TGGATCTACT CCAGCAACTT
    GACTCTAGTA TTGGCATCAC TCTCATTGCT GTGGATGAGG CTCACTGCAT TTCAGAGTGG
    GGCCATGATT TCAGAAGTTC ATTCAGGATG CTGGGCTCTC TTAAAACAGC GCTCCCATTG
    GTTCCAGTCA TTGCACTCTC CGCTACTGCA AGCTCTTCCA TCCGGGAAGA CATTATAAGC
    TGCTTAAACC TGAAAGACCC TCAGATCACC TGCACTGGAT TTGATCGGCC AAATCTGTAC
    TTAGAAGTTG GACGGAAAAC AGGGAACATC CTTCAGGATC TAAAGCCGTT TCTCGTCCGA
    AAGGCAAGTT CTGCCTGGGA ATTTGAAGGT CCAACCATCA TCTATTGTCC TTCGAGAAAA
    ATGACAGAAC AAGTTACTGC TGAACTTGGG AAACTGAACT TAGCCTGCAG AACATACCAC
    GCTGGCATGA AAATTAGCGA AAGGAAGGAC GTTCATCATA GGTTCCTGAG AGATGAAATT
    CAGTGTGTTG TAGCTACTGT AGCTTTTGGA ATGGGCATTA ATAAAGCTGA CATTCGCAAA
    GTTATTCATT ATGGTGCGCC TAAGGAAATG GAATCCTATT ACCAGGAAAT TGGTAGAGCT
    GGCCGGGATG GACTTCAGAG TTCCTGTCAC TTGCTCTGGG CTCCAGCAGA CTTTAACACA
    TCCAGGAATC TCCTTATTGA GATTCACGAT GAAAAGTTCC GGTTATATAA ATTAAAGATG
    ATGGTAAAGA TGGAAAAATA CCTTCACTCC AGTCAGTGTA GGCGACGAAT CATCTTGTCC
    CATTTTGAGG ACAAATGTCT GCAGAAGGCC TCCTTGGACA TTATGGGAAC TGAAAAATGC
    TGTGATAATT GCAGGCCCAG GCTGAATCAT TGCCTTACTG CTAACAACTC AGAGGACGCA
    TCCCAAGACT TTGGGCCACA AGCATTCCAG CTACTGTCTG CTGTGGACAT CCTGCAGGAG
    AAATTTGGAA TTGGGATTCC GATCTTATTT CTCCGAGGAT CTAATTCTCA GCGTCTTCCT
    GATAAATATC GGGGTCACAG GCTCTTTGGT GCTGGAAAGG AGCAAGCAGA AAGTTGGTGG
    AAGACCCTTT CTCACCATCT CATAGCTGAA GGATTCTTGG TAGAAGTTCC CAAGGAAAAC
    AAATATATAA AGACATGTTC CCTCACAAAA AAGGGTAGAA AGTGGCTTGG AGAAGCCAGT
    TCGCAGTCTC CTCCGAGCCT TCTCCTTCAA GCTAATGAAG AGATGTTTCC AAGGAAAGTT
    CTGCTACCAA GTTCTAATCC TGTATCTCCA GAAACGACGC AACATTCCTC TAATCAAAAC
    CCAGCTGGAT TAACTACCAA GCAGTCTAAT TTGGAGAGAA CGCATTCTTA CAAAGTGCCT
    GAGAAAGTTT CTTCTGGGAC TAACATTCCT AAAAAAAGTG CCGTGATGCC GTCACCAGGA
    ACATCTTCCA GCCCCTTAGA ACCTGCCATC TCAGCCCAAG AGCTGGACGC TCGGACTGGG
    CTATATGCCA GGCTGGTGGA AGCAAGGCAG AAACACGCTA ATAAGATGGA TGTACCTCCA
    GCTATTTTAG CAACAAACAA GGTTCTGCTG GACATGGCTA AAATGAGACC GACTACTGTT
    GAAAACATGA AACAGATCGA CGGTGTCTCT GAAGGCAAAG CTGCTCTGTT GGCCCCTCTG
    TTGGAAGTCA TCAAACATTT CTGTCAAGTA ACTAGTGTTC AGACAGACCT CCTTTCCAGT
    GCCAAACCTC ACAAGGAACA GGAGAAAAGT CAGGAGATGG AAAAGAAAGA CTGCTCACTC
    CCCCAGTCTG TGGCCGTCAC ATACACTCTA TTCCAGGAAA AGAAAATGCC CTTACACAGC
    ATAGCTGAGA ACAGGCTCCT GCCTCTCACA GCAGCCGGCA TGCACTTAGC CCAGGCGGTG
    AAAGCCGGCT ACCCCCTGGA TATGGAGCGA GCTGGCCTGA CCCCAGAGAC TTGGAAGATT
    ATTATGGATG TCATCCGAAA CCCTCCCATC AACTCAGATA TGTATAAAGT TAAACTCATC
    AGAATGTTAG TTCCTGAAAA CTTAGACACG TACCTCATCC ACATGGCGAT TGAGATTCTT
    CAGAGTGGTT CCGACAGCAG AACCCAGCCT CCTTGTGATT CCAGCAGGAA GAGGCGTTTC
    CCCAGCTCTG CAGAGAGTTG TGAGAGCTGT AAGGAGAGCA AAGAGGCGGT CACCGAGACC
    AAGGCATCAT CTTCAGAGTC AAAGAGAAAA TTACCCGAGT GGTTTGCCAA AGGAAATGTG
    CCCTCAGCTG ATACCGGCAG CTCATCATCA ATGGCCAAGA CCAAAAAGAA AGGTCTCTTT
    AGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu21413
    Clone ID Related Accession (Same CDS sequence) NM_011721.4 , XM_006509091.2 , XM_006509091.3 , XM_017312662.1 , XM_006509091.4 , XM_017312662.2 , NM_001122822.1
    Accession Version XM_006509091.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4206bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product Werner syndrome ATP-dependent helicase homolog isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006509091.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGAAACCA CTTCACTACA GCGGAAATTT CCAGAATGGA TGTCTATGCA GAGTCAAAGA 
    TGTGCTACAG AAGAAAAGGC CTGCGTTCAG AAGAGTGTTC TTGAAGATAA CCTCCCATTC
    TTAGAATTCC CTGGATCCAT TGTTTACAGT TATGAAGCTA GTGATTGCTC CTTCCTGTCT
    GAAGACATTA GCATGCGTCT GTCTGATGGC GATGTGGTGG GATTTGACAT GGAATGGCCG
    CCCATATACA AGCCAGGGAA AAGAAGCAGA GTCGCAGTGA TCCAGTTGTG TGTGTCTGAG
    AGCAAATGTT ACTTGTTTCA CATTTCTTCC ATGTCAGTTT TCCCCCAGGG ATTAAAAATG
    TTACTAGAAA ACAAATCAAT TAAGAAGGCA GGGGTTGGGA TTGAAGGGGA CCAGTGGAAA
    CTTCTGCGTG ATTTTGACGT CAAGTTGGAG AGTTTTGTGG AGCTGACGGA TGTTGCCAAT
    GAAAAGTTGA AGTGCGCAGA GACCTGGAGC CTCAATGGTC TGGTTAAACA CGTCTTAGGG
    AAACAACTTT TGAAAGACAA GTCCATCCGC TGCAGCAATT GGAGTAATTT CCCCCTCACT
    GAGGACCAGA AACTGTATGC AGCCACTGAT GCTTATGCTG GTCTTATCAT CTATCAAAAA
    TTAGGAAATT TGGGTGATAC TGCGCAAGTG TTTGCTCTAA ATAAAGCAGA GGAAAACCTA
    CCTCTGGAGA TGAAGAAACA GTTGAATTCA ATCTCCGAAG AAATGAGGGA CCTAGCCAAT
    CGTTTTCCTG TCACTTGCAG AAATTTGGAA ACTCTCCAGA GGGTTCCTGT AATATTGAAG
    AGTATTTCAG AAAATCTCTG TTCATTGAGA AAAGTGATCT GTGGTCCTAC AAACACTGAG
    ACTAGACTGA AGCCGGGCAG TAGTTTTAAT TTACTGTCAT CAGAGGATTC AGCTGCTGCT
    GGAGAAAAAG AGAAACAGAT TGGAAAACAT AGTACTTTTG CTAAAATTAA AGAAGAACCA
    TGGGACCCAG AACTTGACAG TTTAGTGAAG CAAGAGGAGG TTGATGTATT TAGAAATCAA
    GTGAAGCAAG AAAAAGGTGA ATCTGAAAAT GAAATAGAAG ACAATCTGTT GAGAGAAGAT
    ATGGAAAGAA CTTGTGTGAT TCCTAGTATT TCAGAAAATG AACTCCAAGA TTTGGAACAG
    CAAGCTAAAG AAGAAAAATA TAATGATGTT TCTCACCAAC TTTCTGAGCA TTTATCTCCC
    AATGATGATG AGAATGACTC CTCCTATATA ATTGAAAGTG ATGAAGATTT GGAAATGGAG
    ATGCTGAAGT CTTTAGAAAA CCTAAATAGT GACGTGGTGG AACCCACTCA CTCTACATGG
    TTGGAAATGG GAACCAATGG GCGTCTTCCT CCTGAGGAGG AAGATGGACA CGGAAATGAA
    GCCATCAAAG AGGAGCAGGA AGAAGAGGAC CATTTATTGC CGGAACCCAA CGCAAAGCAA
    ATTAATTGCC TCAAGACCTA TTTCGGACAC AGCAGTTTTA AACCGGTTCA GTGGAAAGTC
    ATCCATTCTG TATTAGAAGA GAGAAGAGAT AATGTTGTTG TCATGGCAAC TGGATATGGG
    AAGAGTCTGT GCTTCCAGTA TCCGCCTGTT TATACAGGCA AGATTGGCAT TGTCATTTCA
    CCTCTCATTT CCTTAATGGA AGACCAAGTC CTCCAGCTTG AGCTGTCCAA TGTTCCAGCC
    TGTTTACTTG GATCTGCACA GTCAAAAAAT ATTCTAGGAG ATGTTAAATT AGGCAAATAT
    AGGGTCATCT ACATAACTCC AGAGTTCTGT TCTGGTAACT TGGATCTACT CCAGCAACTT
    GACTCTAGTA TTGGCATCAC TCTCATTGCT GTGGATGAGG CTCACTGCAT TTCAGAGTGG
    GGCCATGATT TCAGAAGTTC ATTCAGGATG CTGGGCTCTC TTAAAACAGC GCTCCCATTG
    GTTCCAGTCA TTGCACTCTC CGCTACTGCA AGCTCTTCCA TCCGGGAAGA CATTATAAGC
    TGCTTAAACC TGAAAGACCC TCAGATCACC TGCACTGGAT TTGATCGGCC AAATCTGTAC
    TTAGAAGTTG GACGGAAAAC AGGGAACATC CTTCAGGATC TAAAGCCGTT TCTCGTCCGA
    AAGGCAAGTT CTGCCTGGGA ATTTGAAGGT CCAACCATCA TCTATTGTCC TTCGAGAAAA
    ATGACAGAAC AAGTTACTGC TGAACTTGGG AAACTGAACT TAGCCTGCAG AACATACCAC
    GCTGGCATGA AAATTAGCGA AAGGAAGGAC GTTCATCATA GGTTCCTGAG AGATGAAATT
    CAGTGTGTTG TAGCTACTGT AGCTTTTGGA ATGGGCATTA ATAAAGCTGA CATTCGCAAA
    GTTATTCATT ATGGTGCGCC TAAGGAAATG GAATCCTATT ACCAGGAAAT TGGTAGAGCT
    GGCCGGGATG GACTTCAGAG TTCCTGTCAC TTGCTCTGGG CTCCAGCAGA CTTTAACACA
    TCCAGGAATC TCCTTATTGA GATTCACGAT GAAAAGTTCC GGTTATATAA ATTAAAGATG
    ATGGTAAAGA TGGAAAAATA CCTTCACTCC AGTCAGTGTA GGCGACGAAT CATCTTGTCC
    CATTTTGAGG ACAAATGTCT GCAGAAGGCC TCCTTGGACA TTATGGGAAC TGAAAAATGC
    TGTGATAATT GCAGGCCCAG GCTGAATCAT TGCCTTACTG CTAACAACTC AGAGGACGCA
    TCCCAAGACT TTGGGCCACA AGCATTCCAG CTACTGTCTG CTGTGGACAT CCTGCAGGAG
    AAATTTGGAA TTGGGATTCC GATCTTATTT CTCCGAGGAT CTAATTCTCA GCGTCTTCCT
    GATAAATATC GGGGTCACAG GCTCTTTGGT GCTGGAAAGG AGCAAGCAGA AAGTTGGTGG
    AAGACCCTTT CTCACCATCT CATAGCTGAA GGATTCTTGG TAGAAGTTCC CAAGGAAAAC
    AAATATATAA AGACATGTTC CCTCACAAAA AAGGGTAGAA AGTGGCTTGG AGAAGCCAGT
    TCGCAGTCTC CTCCGAGCCT TCTCCTTCAA GCTAATGAAG AGATGTTTCC AAGGAAAGTT
    CTGCTACCAA GTTCTAATCC TGTATCTCCA GAAACGACGC AACATTCCTC TAATCAAAAC
    CCAGCTGGAT TAACTACCAA GCAGTCTAAT TTGGAGAGAA CGCATTCTTA CAAAGTGCCT
    GAGAAAGTTT CTTCTGGGAC TAACATTCCT AAAAAAAGTG CCGTGATGCC GTCACCAGGA
    ACATCTTCCA GCCCCTTAGA ACCTGCCATC TCAGCCCAAG AGCTGGACGC TCGGACTGGG
    CTATATGCCA GGCTGGTGGA AGCAAGGCAG AAACACGCTA ATAAGATGGA TGTACCTCCA
    GCTATTTTAG CAACAAACAA GGTTCTGCTG GACATGGCTA AAATGAGACC GACTACTGTT
    GAAAACATGA AACAGATCGA CGGTGTCTCT GAAGGCAAAG CTGCTCTGTT GGCCCCTCTG
    TTGGAAGTCA TCAAACATTT CTGTCAAGTA ACTAGTGTTC AGACAGACCT CCTTTCCAGT
    GCCAAACCTC ACAAGGAACA GGAGAAAAGT CAGGAGATGG AAAAGAAAGA CTGCTCACTC
    CCCCAGTCTG TGGCCGTCAC ATACACTCTA TTCCAGGAAA AGAAAATGCC CTTACACAGC
    ATAGCTGAGA ACAGGCTCCT GCCTCTCACA GCAGCCGGCA TGCACTTAGC CCAGGCGGTG
    AAAGCCGGCT ACCCCCTGGA TATGGAGCGA GCTGGCCTGA CCCCAGAGAC TTGGAAGATT
    ATTATGGATG TCATCCGAAA CCCTCCCATC AACTCAGATA TGTATAAAGT TAAACTCATC
    AGAATGTTAG TTCCTGAAAA CTTAGACACG TACCTCATCC ACATGGCGAT TGAGATTCTT
    CAGAGTGGTT CCGACAGCAG AACCCAGCCT CCTTGTGATT CCAGCAGGAA GAGGCGTTTC
    CCCAGCTCTG CAGAGAGTTG TGAGAGCTGT AAGGAGAGCA AAGAGGCGGT CACCGAGACC
    AAGGCATCAT CTTCAGAGTC AAAGAGAAAA TTACCCGAGT GGTTTGCCAA AGGAAATGTG
    CCCTCAGCTG ATACCGGCAG CTCATCATCA ATGGCCAAGA CCAAAAAGAA AGGTCTCTTT
    AGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006509154.1
    CDS331..4536
    Translation

    Target ORF information:

    RefSeq Version XM_006509091.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus Werner syndrome RecQ like helicase (Wrn), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006509091.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGAAACCA CTTCACTACA GCGGAAATTT CCAGAATGGA TGTCTATGCA GAGTCAAAGA 
    TGTGCTACAG AAGAAAAGGC CTGCGTTCAG AAGAGTGTTC TTGAAGATAA CCTCCCATTC
    TTAGAATTCC CTGGATCCAT TGTTTACAGT TATGAAGCTA GTGATTGCTC CTTCCTGTCT
    GAAGACATTA GCATGCGTCT GTCTGATGGC GATGTGGTGG GATTTGACAT GGAATGGCCG
    CCCATATACA AGCCAGGGAA AAGAAGCAGA GTCGCAGTGA TCCAGTTGTG TGTGTCTGAG
    AGCAAATGTT ACTTGTTTCA CATTTCTTCC ATGTCAGTTT TCCCCCAGGG ATTAAAAATG
    TTACTAGAAA ACAAATCAAT TAAGAAGGCA GGGGTTGGGA TTGAAGGGGA CCAGTGGAAA
    CTTCTGCGTG ATTTTGACGT CAAGTTGGAG AGTTTTGTGG AGCTGACGGA TGTTGCCAAT
    GAAAAGTTGA AGTGCGCAGA GACCTGGAGC CTCAATGGTC TGGTTAAACA CGTCTTAGGG
    AAACAACTTT TGAAAGACAA GTCCATCCGC TGCAGCAATT GGAGTAATTT CCCCCTCACT
    GAGGACCAGA AACTGTATGC AGCCACTGAT GCTTATGCTG GTCTTATCAT CTATCAAAAA
    TTAGGAAATT TGGGTGATAC TGCGCAAGTG TTTGCTCTAA ATAAAGCAGA GGAAAACCTA
    CCTCTGGAGA TGAAGAAACA GTTGAATTCA ATCTCCGAAG AAATGAGGGA CCTAGCCAAT
    CGTTTTCCTG TCACTTGCAG AAATTTGGAA ACTCTCCAGA GGGTTCCTGT AATATTGAAG
    AGTATTTCAG AAAATCTCTG TTCATTGAGA AAAGTGATCT GTGGTCCTAC AAACACTGAG
    ACTAGACTGA AGCCGGGCAG TAGTTTTAAT TTACTGTCAT CAGAGGATTC AGCTGCTGCT
    GGAGAAAAAG AGAAACAGAT TGGAAAACAT AGTACTTTTG CTAAAATTAA AGAAGAACCA
    TGGGACCCAG AACTTGACAG TTTAGTGAAG CAAGAGGAGG TTGATGTATT TAGAAATCAA
    GTGAAGCAAG AAAAAGGTGA ATCTGAAAAT GAAATAGAAG ACAATCTGTT GAGAGAAGAT
    ATGGAAAGAA CTTGTGTGAT TCCTAGTATT TCAGAAAATG AACTCCAAGA TTTGGAACAG
    CAAGCTAAAG AAGAAAAATA TAATGATGTT TCTCACCAAC TTTCTGAGCA TTTATCTCCC
    AATGATGATG AGAATGACTC CTCCTATATA ATTGAAAGTG ATGAAGATTT GGAAATGGAG
    ATGCTGAAGT CTTTAGAAAA CCTAAATAGT GACGTGGTGG AACCCACTCA CTCTACATGG
    TTGGAAATGG GAACCAATGG GCGTCTTCCT CCTGAGGAGG AAGATGGACA CGGAAATGAA
    GCCATCAAAG AGGAGCAGGA AGAAGAGGAC CATTTATTGC CGGAACCCAA CGCAAAGCAA
    ATTAATTGCC TCAAGACCTA TTTCGGACAC AGCAGTTTTA AACCGGTTCA GTGGAAAGTC
    ATCCATTCTG TATTAGAAGA GAGAAGAGAT AATGTTGTTG TCATGGCAAC TGGATATGGG
    AAGAGTCTGT GCTTCCAGTA TCCGCCTGTT TATACAGGCA AGATTGGCAT TGTCATTTCA
    CCTCTCATTT CCTTAATGGA AGACCAAGTC CTCCAGCTTG AGCTGTCCAA TGTTCCAGCC
    TGTTTACTTG GATCTGCACA GTCAAAAAAT ATTCTAGGAG ATGTTAAATT AGGCAAATAT
    AGGGTCATCT ACATAACTCC AGAGTTCTGT TCTGGTAACT TGGATCTACT CCAGCAACTT
    GACTCTAGTA TTGGCATCAC TCTCATTGCT GTGGATGAGG CTCACTGCAT TTCAGAGTGG
    GGCCATGATT TCAGAAGTTC ATTCAGGATG CTGGGCTCTC TTAAAACAGC GCTCCCATTG
    GTTCCAGTCA TTGCACTCTC CGCTACTGCA AGCTCTTCCA TCCGGGAAGA CATTATAAGC
    TGCTTAAACC TGAAAGACCC TCAGATCACC TGCACTGGAT TTGATCGGCC AAATCTGTAC
    TTAGAAGTTG GACGGAAAAC AGGGAACATC CTTCAGGATC TAAAGCCGTT TCTCGTCCGA
    AAGGCAAGTT CTGCCTGGGA ATTTGAAGGT CCAACCATCA TCTATTGTCC TTCGAGAAAA
    ATGACAGAAC AAGTTACTGC TGAACTTGGG AAACTGAACT TAGCCTGCAG AACATACCAC
    GCTGGCATGA AAATTAGCGA AAGGAAGGAC GTTCATCATA GGTTCCTGAG AGATGAAATT
    CAGTGTGTTG TAGCTACTGT AGCTTTTGGA ATGGGCATTA ATAAAGCTGA CATTCGCAAA
    GTTATTCATT ATGGTGCGCC TAAGGAAATG GAATCCTATT ACCAGGAAAT TGGTAGAGCT
    GGCCGGGATG GACTTCAGAG TTCCTGTCAC TTGCTCTGGG CTCCAGCAGA CTTTAACACA
    TCCAGGAATC TCCTTATTGA GATTCACGAT GAAAAGTTCC GGTTATATAA ATTAAAGATG
    ATGGTAAAGA TGGAAAAATA CCTTCACTCC AGTCAGTGTA GGCGACGAAT CATCTTGTCC
    CATTTTGAGG ACAAATGTCT GCAGAAGGCC TCCTTGGACA TTATGGGAAC TGAAAAATGC
    TGTGATAATT GCAGGCCCAG GCTGAATCAT TGCCTTACTG CTAACAACTC AGAGGACGCA
    TCCCAAGACT TTGGGCCACA AGCATTCCAG CTACTGTCTG CTGTGGACAT CCTGCAGGAG
    AAATTTGGAA TTGGGATTCC GATCTTATTT CTCCGAGGAT CTAATTCTCA GCGTCTTCCT
    GATAAATATC GGGGTCACAG GCTCTTTGGT GCTGGAAAGG AGCAAGCAGA AAGTTGGTGG
    AAGACCCTTT CTCACCATCT CATAGCTGAA GGATTCTTGG TAGAAGTTCC CAAGGAAAAC
    AAATATATAA AGACATGTTC CCTCACAAAA AAGGGTAGAA AGTGGCTTGG AGAAGCCAGT
    TCGCAGTCTC CTCCGAGCCT TCTCCTTCAA GCTAATGAAG AGATGTTTCC AAGGAAAGTT
    CTGCTACCAA GTTCTAATCC TGTATCTCCA GAAACGACGC AACATTCCTC TAATCAAAAC
    CCAGCTGGAT TAACTACCAA GCAGTCTAAT TTGGAGAGAA CGCATTCTTA CAAAGTGCCT
    GAGAAAGTTT CTTCTGGGAC TAACATTCCT AAAAAAAGTG CCGTGATGCC GTCACCAGGA
    ACATCTTCCA GCCCCTTAGA ACCTGCCATC TCAGCCCAAG AGCTGGACGC TCGGACTGGG
    CTATATGCCA GGCTGGTGGA AGCAAGGCAG AAACACGCTA ATAAGATGGA TGTACCTCCA
    GCTATTTTAG CAACAAACAA GGTTCTGCTG GACATGGCTA AAATGAGACC GACTACTGTT
    GAAAACATGA AACAGATCGA CGGTGTCTCT GAAGGCAAAG CTGCTCTGTT GGCCCCTCTG
    TTGGAAGTCA TCAAACATTT CTGTCAAGTA ACTAGTGTTC AGACAGACCT CCTTTCCAGT
    GCCAAACCTC ACAAGGAACA GGAGAAAAGT CAGGAGATGG AAAAGAAAGA CTGCTCACTC
    CCCCAGTCTG TGGCCGTCAC ATACACTCTA TTCCAGGAAA AGAAAATGCC CTTACACAGC
    ATAGCTGAGA ACAGGCTCCT GCCTCTCACA GCAGCCGGCA TGCACTTAGC CCAGGCGGTG
    AAAGCCGGCT ACCCCCTGGA TATGGAGCGA GCTGGCCTGA CCCCAGAGAC TTGGAAGATT
    ATTATGGATG TCATCCGAAA CCCTCCCATC AACTCAGATA TGTATAAAGT TAAACTCATC
    AGAATGTTAG TTCCTGAAAA CTTAGACACG TACCTCATCC ACATGGCGAT TGAGATTCTT
    CAGAGTGGTT CCGACAGCAG AACCCAGCCT CCTTGTGATT CCAGCAGGAA GAGGCGTTTC
    CCCAGCTCTG CAGAGAGTTG TGAGAGCTGT AAGGAGAGCA AAGAGGCGGT CACCGAGACC
    AAGGCATCAT CTTCAGAGTC AAAGAGAAAA TTACCCGAGT GGTTTGCCAA AGGAAATGTG
    CCCTCAGCTG ATACCGGCAG CTCATCATCA ATGGCCAAGA CCAAAAAGAA AGGTCTCTTT
    AGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu21413
    Clone ID Related Accession (Same CDS sequence) NM_011721.4 , XM_006509091.2 , XM_006509091.3 , XM_017312662.1 , XM_006509091.4 , XM_017312662.2 , NM_001122822.1
    Accession Version XM_017312662.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4206bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product Werner syndrome ATP-dependent helicase homolog isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017312662.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGAAACCA CTTCACTACA GCGGAAATTT CCAGAATGGA TGTCTATGCA GAGTCAAAGA 
    TGTGCTACAG AAGAAAAGGC CTGCGTTCAG AAGAGTGTTC TTGAAGATAA CCTCCCATTC
    TTAGAATTCC CTGGATCCAT TGTTTACAGT TATGAAGCTA GTGATTGCTC CTTCCTGTCT
    GAAGACATTA GCATGCGTCT GTCTGATGGC GATGTGGTGG GATTTGACAT GGAATGGCCG
    CCCATATACA AGCCAGGGAA AAGAAGCAGA GTCGCAGTGA TCCAGTTGTG TGTGTCTGAG
    AGCAAATGTT ACTTGTTTCA CATTTCTTCC ATGTCAGTTT TCCCCCAGGG ATTAAAAATG
    TTACTAGAAA ACAAATCAAT TAAGAAGGCA GGGGTTGGGA TTGAAGGGGA CCAGTGGAAA
    CTTCTGCGTG ATTTTGACGT CAAGTTGGAG AGTTTTGTGG AGCTGACGGA TGTTGCCAAT
    GAAAAGTTGA AGTGCGCAGA GACCTGGAGC CTCAATGGTC TGGTTAAACA CGTCTTAGGG
    AAACAACTTT TGAAAGACAA GTCCATCCGC TGCAGCAATT GGAGTAATTT CCCCCTCACT
    GAGGACCAGA AACTGTATGC AGCCACTGAT GCTTATGCTG GTCTTATCAT CTATCAAAAA
    TTAGGAAATT TGGGTGATAC TGCGCAAGTG TTTGCTCTAA ATAAAGCAGA GGAAAACCTA
    CCTCTGGAGA TGAAGAAACA GTTGAATTCA ATCTCCGAAG AAATGAGGGA CCTAGCCAAT
    CGTTTTCCTG TCACTTGCAG AAATTTGGAA ACTCTCCAGA GGGTTCCTGT AATATTGAAG
    AGTATTTCAG AAAATCTCTG TTCATTGAGA AAAGTGATCT GTGGTCCTAC AAACACTGAG
    ACTAGACTGA AGCCGGGCAG TAGTTTTAAT TTACTGTCAT CAGAGGATTC AGCTGCTGCT
    GGAGAAAAAG AGAAACAGAT TGGAAAACAT AGTACTTTTG CTAAAATTAA AGAAGAACCA
    TGGGACCCAG AACTTGACAG TTTAGTGAAG CAAGAGGAGG TTGATGTATT TAGAAATCAA
    GTGAAGCAAG AAAAAGGTGA ATCTGAAAAT GAAATAGAAG ACAATCTGTT GAGAGAAGAT
    ATGGAAAGAA CTTGTGTGAT TCCTAGTATT TCAGAAAATG AACTCCAAGA TTTGGAACAG
    CAAGCTAAAG AAGAAAAATA TAATGATGTT TCTCACCAAC TTTCTGAGCA TTTATCTCCC
    AATGATGATG AGAATGACTC CTCCTATATA ATTGAAAGTG ATGAAGATTT GGAAATGGAG
    ATGCTGAAGT CTTTAGAAAA CCTAAATAGT GACGTGGTGG AACCCACTCA CTCTACATGG
    TTGGAAATGG GAACCAATGG GCGTCTTCCT CCTGAGGAGG AAGATGGACA CGGAAATGAA
    GCCATCAAAG AGGAGCAGGA AGAAGAGGAC CATTTATTGC CGGAACCCAA CGCAAAGCAA
    ATTAATTGCC TCAAGACCTA TTTCGGACAC AGCAGTTTTA AACCGGTTCA GTGGAAAGTC
    ATCCATTCTG TATTAGAAGA GAGAAGAGAT AATGTTGTTG TCATGGCAAC TGGATATGGG
    AAGAGTCTGT GCTTCCAGTA TCCGCCTGTT TATACAGGCA AGATTGGCAT TGTCATTTCA
    CCTCTCATTT CCTTAATGGA AGACCAAGTC CTCCAGCTTG AGCTGTCCAA TGTTCCAGCC
    TGTTTACTTG GATCTGCACA GTCAAAAAAT ATTCTAGGAG ATGTTAAATT AGGCAAATAT
    AGGGTCATCT ACATAACTCC AGAGTTCTGT TCTGGTAACT TGGATCTACT CCAGCAACTT
    GACTCTAGTA TTGGCATCAC TCTCATTGCT GTGGATGAGG CTCACTGCAT TTCAGAGTGG
    GGCCATGATT TCAGAAGTTC ATTCAGGATG CTGGGCTCTC TTAAAACAGC GCTCCCATTG
    GTTCCAGTCA TTGCACTCTC CGCTACTGCA AGCTCTTCCA TCCGGGAAGA CATTATAAGC
    TGCTTAAACC TGAAAGACCC TCAGATCACC TGCACTGGAT TTGATCGGCC AAATCTGTAC
    TTAGAAGTTG GACGGAAAAC AGGGAACATC CTTCAGGATC TAAAGCCGTT TCTCGTCCGA
    AAGGCAAGTT CTGCCTGGGA ATTTGAAGGT CCAACCATCA TCTATTGTCC TTCGAGAAAA
    ATGACAGAAC AAGTTACTGC TGAACTTGGG AAACTGAACT TAGCCTGCAG AACATACCAC
    GCTGGCATGA AAATTAGCGA AAGGAAGGAC GTTCATCATA GGTTCCTGAG AGATGAAATT
    CAGTGTGTTG TAGCTACTGT AGCTTTTGGA ATGGGCATTA ATAAAGCTGA CATTCGCAAA
    GTTATTCATT ATGGTGCGCC TAAGGAAATG GAATCCTATT ACCAGGAAAT TGGTAGAGCT
    GGCCGGGATG GACTTCAGAG TTCCTGTCAC TTGCTCTGGG CTCCAGCAGA CTTTAACACA
    TCCAGGAATC TCCTTATTGA GATTCACGAT GAAAAGTTCC GGTTATATAA ATTAAAGATG
    ATGGTAAAGA TGGAAAAATA CCTTCACTCC AGTCAGTGTA GGCGACGAAT CATCTTGTCC
    CATTTTGAGG ACAAATGTCT GCAGAAGGCC TCCTTGGACA TTATGGGAAC TGAAAAATGC
    TGTGATAATT GCAGGCCCAG GCTGAATCAT TGCCTTACTG CTAACAACTC AGAGGACGCA
    TCCCAAGACT TTGGGCCACA AGCATTCCAG CTACTGTCTG CTGTGGACAT CCTGCAGGAG
    AAATTTGGAA TTGGGATTCC GATCTTATTT CTCCGAGGAT CTAATTCTCA GCGTCTTCCT
    GATAAATATC GGGGTCACAG GCTCTTTGGT GCTGGAAAGG AGCAAGCAGA AAGTTGGTGG
    AAGACCCTTT CTCACCATCT CATAGCTGAA GGATTCTTGG TAGAAGTTCC CAAGGAAAAC
    AAATATATAA AGACATGTTC CCTCACAAAA AAGGGTAGAA AGTGGCTTGG AGAAGCCAGT
    TCGCAGTCTC CTCCGAGCCT TCTCCTTCAA GCTAATGAAG AGATGTTTCC AAGGAAAGTT
    CTGCTACCAA GTTCTAATCC TGTATCTCCA GAAACGACGC AACATTCCTC TAATCAAAAC
    CCAGCTGGAT TAACTACCAA GCAGTCTAAT TTGGAGAGAA CGCATTCTTA CAAAGTGCCT
    GAGAAAGTTT CTTCTGGGAC TAACATTCCT AAAAAAAGTG CCGTGATGCC GTCACCAGGA
    ACATCTTCCA GCCCCTTAGA ACCTGCCATC TCAGCCCAAG AGCTGGACGC TCGGACTGGG
    CTATATGCCA GGCTGGTGGA AGCAAGGCAG AAACACGCTA ATAAGATGGA TGTACCTCCA
    GCTATTTTAG CAACAAACAA GGTTCTGCTG GACATGGCTA AAATGAGACC GACTACTGTT
    GAAAACATGA AACAGATCGA CGGTGTCTCT GAAGGCAAAG CTGCTCTGTT GGCCCCTCTG
    TTGGAAGTCA TCAAACATTT CTGTCAAGTA ACTAGTGTTC AGACAGACCT CCTTTCCAGT
    GCCAAACCTC ACAAGGAACA GGAGAAAAGT CAGGAGATGG AAAAGAAAGA CTGCTCACTC
    CCCCAGTCTG TGGCCGTCAC ATACACTCTA TTCCAGGAAA AGAAAATGCC CTTACACAGC
    ATAGCTGAGA ACAGGCTCCT GCCTCTCACA GCAGCCGGCA TGCACTTAGC CCAGGCGGTG
    AAAGCCGGCT ACCCCCTGGA TATGGAGCGA GCTGGCCTGA CCCCAGAGAC TTGGAAGATT
    ATTATGGATG TCATCCGAAA CCCTCCCATC AACTCAGATA TGTATAAAGT TAAACTCATC
    AGAATGTTAG TTCCTGAAAA CTTAGACACG TACCTCATCC ACATGGCGAT TGAGATTCTT
    CAGAGTGGTT CCGACAGCAG AACCCAGCCT CCTTGTGATT CCAGCAGGAA GAGGCGTTTC
    CCCAGCTCTG CAGAGAGTTG TGAGAGCTGT AAGGAGAGCA AAGAGGCGGT CACCGAGACC
    AAGGCATCAT CTTCAGAGTC AAAGAGAAAA TTACCCGAGT GGTTTGCCAA AGGAAATGTG
    CCCTCAGCTG ATACCGGCAG CTCATCATCA ATGGCCAAGA CCAAAAAGAA AGGTCTCTTT
    AGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017168151.1
    CDS385..4590
    Translation

    Target ORF information:

    RefSeq Version XM_017312662.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus Werner syndrome RecQ like helicase (Wrn), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017312662.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGAAACCA CTTCACTACA GCGGAAATTT CCAGAATGGA TGTCTATGCA GAGTCAAAGA 
    TGTGCTACAG AAGAAAAGGC CTGCGTTCAG AAGAGTGTTC TTGAAGATAA CCTCCCATTC
    TTAGAATTCC CTGGATCCAT TGTTTACAGT TATGAAGCTA GTGATTGCTC CTTCCTGTCT
    GAAGACATTA GCATGCGTCT GTCTGATGGC GATGTGGTGG GATTTGACAT GGAATGGCCG
    CCCATATACA AGCCAGGGAA AAGAAGCAGA GTCGCAGTGA TCCAGTTGTG TGTGTCTGAG
    AGCAAATGTT ACTTGTTTCA CATTTCTTCC ATGTCAGTTT TCCCCCAGGG ATTAAAAATG
    TTACTAGAAA ACAAATCAAT TAAGAAGGCA GGGGTTGGGA TTGAAGGGGA CCAGTGGAAA
    CTTCTGCGTG ATTTTGACGT CAAGTTGGAG AGTTTTGTGG AGCTGACGGA TGTTGCCAAT
    GAAAAGTTGA AGTGCGCAGA GACCTGGAGC CTCAATGGTC TGGTTAAACA CGTCTTAGGG
    AAACAACTTT TGAAAGACAA GTCCATCCGC TGCAGCAATT GGAGTAATTT CCCCCTCACT
    GAGGACCAGA AACTGTATGC AGCCACTGAT GCTTATGCTG GTCTTATCAT CTATCAAAAA
    TTAGGAAATT TGGGTGATAC TGCGCAAGTG TTTGCTCTAA ATAAAGCAGA GGAAAACCTA
    CCTCTGGAGA TGAAGAAACA GTTGAATTCA ATCTCCGAAG AAATGAGGGA CCTAGCCAAT
    CGTTTTCCTG TCACTTGCAG AAATTTGGAA ACTCTCCAGA GGGTTCCTGT AATATTGAAG
    AGTATTTCAG AAAATCTCTG TTCATTGAGA AAAGTGATCT GTGGTCCTAC AAACACTGAG
    ACTAGACTGA AGCCGGGCAG TAGTTTTAAT TTACTGTCAT CAGAGGATTC AGCTGCTGCT
    GGAGAAAAAG AGAAACAGAT TGGAAAACAT AGTACTTTTG CTAAAATTAA AGAAGAACCA
    TGGGACCCAG AACTTGACAG TTTAGTGAAG CAAGAGGAGG TTGATGTATT TAGAAATCAA
    GTGAAGCAAG AAAAAGGTGA ATCTGAAAAT GAAATAGAAG ACAATCTGTT GAGAGAAGAT
    ATGGAAAGAA CTTGTGTGAT TCCTAGTATT TCAGAAAATG AACTCCAAGA TTTGGAACAG
    CAAGCTAAAG AAGAAAAATA TAATGATGTT TCTCACCAAC TTTCTGAGCA TTTATCTCCC
    AATGATGATG AGAATGACTC CTCCTATATA ATTGAAAGTG ATGAAGATTT GGAAATGGAG
    ATGCTGAAGT CTTTAGAAAA CCTAAATAGT GACGTGGTGG AACCCACTCA CTCTACATGG
    TTGGAAATGG GAACCAATGG GCGTCTTCCT CCTGAGGAGG AAGATGGACA CGGAAATGAA
    GCCATCAAAG AGGAGCAGGA AGAAGAGGAC CATTTATTGC CGGAACCCAA CGCAAAGCAA
    ATTAATTGCC TCAAGACCTA TTTCGGACAC AGCAGTTTTA AACCGGTTCA GTGGAAAGTC
    ATCCATTCTG TATTAGAAGA GAGAAGAGAT AATGTTGTTG TCATGGCAAC TGGATATGGG
    AAGAGTCTGT GCTTCCAGTA TCCGCCTGTT TATACAGGCA AGATTGGCAT TGTCATTTCA
    CCTCTCATTT CCTTAATGGA AGACCAAGTC CTCCAGCTTG AGCTGTCCAA TGTTCCAGCC
    TGTTTACTTG GATCTGCACA GTCAAAAAAT ATTCTAGGAG ATGTTAAATT AGGCAAATAT
    AGGGTCATCT ACATAACTCC AGAGTTCTGT TCTGGTAACT TGGATCTACT CCAGCAACTT
    GACTCTAGTA TTGGCATCAC TCTCATTGCT GTGGATGAGG CTCACTGCAT TTCAGAGTGG
    GGCCATGATT TCAGAAGTTC ATTCAGGATG CTGGGCTCTC TTAAAACAGC GCTCCCATTG
    GTTCCAGTCA TTGCACTCTC CGCTACTGCA AGCTCTTCCA TCCGGGAAGA CATTATAAGC
    TGCTTAAACC TGAAAGACCC TCAGATCACC TGCACTGGAT TTGATCGGCC AAATCTGTAC
    TTAGAAGTTG GACGGAAAAC AGGGAACATC CTTCAGGATC TAAAGCCGTT TCTCGTCCGA
    AAGGCAAGTT CTGCCTGGGA ATTTGAAGGT CCAACCATCA TCTATTGTCC TTCGAGAAAA
    ATGACAGAAC AAGTTACTGC TGAACTTGGG AAACTGAACT TAGCCTGCAG AACATACCAC
    GCTGGCATGA AAATTAGCGA AAGGAAGGAC GTTCATCATA GGTTCCTGAG AGATGAAATT
    CAGTGTGTTG TAGCTACTGT AGCTTTTGGA ATGGGCATTA ATAAAGCTGA CATTCGCAAA
    GTTATTCATT ATGGTGCGCC TAAGGAAATG GAATCCTATT ACCAGGAAAT TGGTAGAGCT
    GGCCGGGATG GACTTCAGAG TTCCTGTCAC TTGCTCTGGG CTCCAGCAGA CTTTAACACA
    TCCAGGAATC TCCTTATTGA GATTCACGAT GAAAAGTTCC GGTTATATAA ATTAAAGATG
    ATGGTAAAGA TGGAAAAATA CCTTCACTCC AGTCAGTGTA GGCGACGAAT CATCTTGTCC
    CATTTTGAGG ACAAATGTCT GCAGAAGGCC TCCTTGGACA TTATGGGAAC TGAAAAATGC
    TGTGATAATT GCAGGCCCAG GCTGAATCAT TGCCTTACTG CTAACAACTC AGAGGACGCA
    TCCCAAGACT TTGGGCCACA AGCATTCCAG CTACTGTCTG CTGTGGACAT CCTGCAGGAG
    AAATTTGGAA TTGGGATTCC GATCTTATTT CTCCGAGGAT CTAATTCTCA GCGTCTTCCT
    GATAAATATC GGGGTCACAG GCTCTTTGGT GCTGGAAAGG AGCAAGCAGA AAGTTGGTGG
    AAGACCCTTT CTCACCATCT CATAGCTGAA GGATTCTTGG TAGAAGTTCC CAAGGAAAAC
    AAATATATAA AGACATGTTC CCTCACAAAA AAGGGTAGAA AGTGGCTTGG AGAAGCCAGT
    TCGCAGTCTC CTCCGAGCCT TCTCCTTCAA GCTAATGAAG AGATGTTTCC AAGGAAAGTT
    CTGCTACCAA GTTCTAATCC TGTATCTCCA GAAACGACGC AACATTCCTC TAATCAAAAC
    CCAGCTGGAT TAACTACCAA GCAGTCTAAT TTGGAGAGAA CGCATTCTTA CAAAGTGCCT
    GAGAAAGTTT CTTCTGGGAC TAACATTCCT AAAAAAAGTG CCGTGATGCC GTCACCAGGA
    ACATCTTCCA GCCCCTTAGA ACCTGCCATC TCAGCCCAAG AGCTGGACGC TCGGACTGGG
    CTATATGCCA GGCTGGTGGA AGCAAGGCAG AAACACGCTA ATAAGATGGA TGTACCTCCA
    GCTATTTTAG CAACAAACAA GGTTCTGCTG GACATGGCTA AAATGAGACC GACTACTGTT
    GAAAACATGA AACAGATCGA CGGTGTCTCT GAAGGCAAAG CTGCTCTGTT GGCCCCTCTG
    TTGGAAGTCA TCAAACATTT CTGTCAAGTA ACTAGTGTTC AGACAGACCT CCTTTCCAGT
    GCCAAACCTC ACAAGGAACA GGAGAAAAGT CAGGAGATGG AAAAGAAAGA CTGCTCACTC
    CCCCAGTCTG TGGCCGTCAC ATACACTCTA TTCCAGGAAA AGAAAATGCC CTTACACAGC
    ATAGCTGAGA ACAGGCTCCT GCCTCTCACA GCAGCCGGCA TGCACTTAGC CCAGGCGGTG
    AAAGCCGGCT ACCCCCTGGA TATGGAGCGA GCTGGCCTGA CCCCAGAGAC TTGGAAGATT
    ATTATGGATG TCATCCGAAA CCCTCCCATC AACTCAGATA TGTATAAAGT TAAACTCATC
    AGAATGTTAG TTCCTGAAAA CTTAGACACG TACCTCATCC ACATGGCGAT TGAGATTCTT
    CAGAGTGGTT CCGACAGCAG AACCCAGCCT CCTTGTGATT CCAGCAGGAA GAGGCGTTTC
    CCCAGCTCTG CAGAGAGTTG TGAGAGCTGT AAGGAGAGCA AAGAGGCGGT CACCGAGACC
    AAGGCATCAT CTTCAGAGTC AAAGAGAAAA TTACCCGAGT GGTTTGCCAA AGGAAATGTG
    CCCTCAGCTG ATACCGGCAG CTCATCATCA ATGGCCAAGA CCAAAAAGAA AGGTCTCTTT
    AGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu40624
    Clone ID Related Accession (Same CDS sequence) XM_006509093.3 , XM_006509093.4
    Accession Version XM_006509093.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4203bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product Werner syndrome ATP-dependent helicase homolog isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006509093.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGAAACCA CTTCACTACA GCGGAAATTT CCAGAATGGA TGTCTATGCA GAGTCAAAGA 
    TGTGCTACAG AAGAAAAGGC CTGCGTTCAG AAGAGTGTTC TTGAAGATAA CCTCCCATTC
    TTAGAATTCC CTGGATCCAT TGTTTACAGT TATGAAGCTA GTGATTGCTC CTTCCTGTCT
    GAAGACATTA GCATGCGTCT GTCTGATGGC GATGTGGTGG GATTTGACAT GGAATGGCCG
    CCCATATACA AGCCAGGGAA AAGAAGCAGA GTCGCAGTGA TCCAGTTGTG TGTGTCTGAG
    AGCAAATGTT ACTTGTTTCA CATTTCTTCC ATGTCAGTTT TCCCCCAGGG ATTAAAAATG
    TTACTAGAAA ACAAATCAAT TAAGAAGGCA GGGGTTGGGA TTGAAGGGGA CCAGTGGAAA
    CTTCTGCGTG ATTTTGACGT CAAGTTGGAG AGTTTTGTGG AGCTGACGGA TGTTGCCAAT
    GAAAAGTTGA AGTGCGCAGA GACCTGGAGC CTCAATGGTC TGGTTAAACA CGTCTTAGGG
    AAACAACTTT TGAAAGACAA GTCCATCCGC TGCAGCAATT GGAGTAATTT CCCCCTCACT
    GAGGACCAGA AACTGTATGC AGCCACTGAT GCTTATGCTG GTCTTATCAT CTATCAAAAA
    TTAGGAAATT TGGGTGATAC TGCGCAAGTG TTTGCTCTAA ATAAAGAGGA AAACCTACCT
    CTGGAGATGA AGAAACAGTT GAATTCAATC TCCGAAGAAA TGAGGGACCT AGCCAATCGT
    TTTCCTGTCA CTTGCAGAAA TTTGGAAACT CTCCAGAGGG TTCCTGTAAT ATTGAAGAGT
    ATTTCAGAAA ATCTCTGTTC ATTGAGAAAA GTGATCTGTG GTCCTACAAA CACTGAGACT
    AGACTGAAGC CGGGCAGTAG TTTTAATTTA CTGTCATCAG AGGATTCAGC TGCTGCTGGA
    GAAAAAGAGA AACAGATTGG AAAACATAGT ACTTTTGCTA AAATTAAAGA AGAACCATGG
    GACCCAGAAC TTGACAGTTT AGTGAAGCAA GAGGAGGTTG ATGTATTTAG AAATCAAGTG
    AAGCAAGAAA AAGGTGAATC TGAAAATGAA ATAGAAGACA ATCTGTTGAG AGAAGATATG
    GAAAGAACTT GTGTGATTCC TAGTATTTCA GAAAATGAAC TCCAAGATTT GGAACAGCAA
    GCTAAAGAAG AAAAATATAA TGATGTTTCT CACCAACTTT CTGAGCATTT ATCTCCCAAT
    GATGATGAGA ATGACTCCTC CTATATAATT GAAAGTGATG AAGATTTGGA AATGGAGATG
    CTGAAGTCTT TAGAAAACCT AAATAGTGAC GTGGTGGAAC CCACTCACTC TACATGGTTG
    GAAATGGGAA CCAATGGGCG TCTTCCTCCT GAGGAGGAAG ATGGACACGG AAATGAAGCC
    ATCAAAGAGG AGCAGGAAGA AGAGGACCAT TTATTGCCGG AACCCAACGC AAAGCAAATT
    AATTGCCTCA AGACCTATTT CGGACACAGC AGTTTTAAAC CGGTTCAGTG GAAAGTCATC
    CATTCTGTAT TAGAAGAGAG AAGAGATAAT GTTGTTGTCA TGGCAACTGG ATATGGGAAG
    AGTCTGTGCT TCCAGTATCC GCCTGTTTAT ACAGGCAAGA TTGGCATTGT CATTTCACCT
    CTCATTTCCT TAATGGAAGA CCAAGTCCTC CAGCTTGAGC TGTCCAATGT TCCAGCCTGT
    TTACTTGGAT CTGCACAGTC AAAAAATATT CTAGGAGATG TTAAATTAGG CAAATATAGG
    GTCATCTACA TAACTCCAGA GTTCTGTTCT GGTAACTTGG ATCTACTCCA GCAACTTGAC
    TCTAGTATTG GCATCACTCT CATTGCTGTG GATGAGGCTC ACTGCATTTC AGAGTGGGGC
    CATGATTTCA GAAGTTCATT CAGGATGCTG GGCTCTCTTA AAACAGCGCT CCCATTGGTT
    CCAGTCATTG CACTCTCCGC TACTGCAAGC TCTTCCATCC GGGAAGACAT TATAAGCTGC
    TTAAACCTGA AAGACCCTCA GATCACCTGC ACTGGATTTG ATCGGCCAAA TCTGTACTTA
    GAAGTTGGAC GGAAAACAGG GAACATCCTT CAGGATCTAA AGCCGTTTCT CGTCCGAAAG
    GCAAGTTCTG CCTGGGAATT TGAAGGTCCA ACCATCATCT ATTGTCCTTC GAGAAAAATG
    ACAGAACAAG TTACTGCTGA ACTTGGGAAA CTGAACTTAG CCTGCAGAAC ATACCACGCT
    GGCATGAAAA TTAGCGAAAG GAAGGACGTT CATCATAGGT TCCTGAGAGA TGAAATTCAG
    TGTGTTGTAG CTACTGTAGC TTTTGGAATG GGCATTAATA AAGCTGACAT TCGCAAAGTT
    ATTCATTATG GTGCGCCTAA GGAAATGGAA TCCTATTACC AGGAAATTGG TAGAGCTGGC
    CGGGATGGAC TTCAGAGTTC CTGTCACTTG CTCTGGGCTC CAGCAGACTT TAACACATCC
    AGGAATCTCC TTATTGAGAT TCACGATGAA AAGTTCCGGT TATATAAATT AAAGATGATG
    GTAAAGATGG AAAAATACCT TCACTCCAGT CAGTGTAGGC GACGAATCAT CTTGTCCCAT
    TTTGAGGACA AATGTCTGCA GAAGGCCTCC TTGGACATTA TGGGAACTGA AAAATGCTGT
    GATAATTGCA GGCCCAGGCT GAATCATTGC CTTACTGCTA ACAACTCAGA GGACGCATCC
    CAAGACTTTG GGCCACAAGC ATTCCAGCTA CTGTCTGCTG TGGACATCCT GCAGGAGAAA
    TTTGGAATTG GGATTCCGAT CTTATTTCTC CGAGGATCTA ATTCTCAGCG TCTTCCTGAT
    AAATATCGGG GTCACAGGCT CTTTGGTGCT GGAAAGGAGC AAGCAGAAAG TTGGTGGAAG
    ACCCTTTCTC ACCATCTCAT AGCTGAAGGA TTCTTGGTAG AAGTTCCCAA GGAAAACAAA
    TATATAAAGA CATGTTCCCT CACAAAAAAG GGTAGAAAGT GGCTTGGAGA AGCCAGTTCG
    CAGTCTCCTC CGAGCCTTCT CCTTCAAGCT AATGAAGAGA TGTTTCCAAG GAAAGTTCTG
    CTACCAAGTT CTAATCCTGT ATCTCCAGAA ACGACGCAAC ATTCCTCTAA TCAAAACCCA
    GCTGGATTAA CTACCAAGCA GTCTAATTTG GAGAGAACGC ATTCTTACAA AGTGCCTGAG
    AAAGTTTCTT CTGGGACTAA CATTCCTAAA AAAAGTGCCG TGATGCCGTC ACCAGGAACA
    TCTTCCAGCC CCTTAGAACC TGCCATCTCA GCCCAAGAGC TGGACGCTCG GACTGGGCTA
    TATGCCAGGC TGGTGGAAGC AAGGCAGAAA CACGCTAATA AGATGGATGT ACCTCCAGCT
    ATTTTAGCAA CAAACAAGGT TCTGCTGGAC ATGGCTAAAA TGAGACCGAC TACTGTTGAA
    AACATGAAAC AGATCGACGG TGTCTCTGAA GGCAAAGCTG CTCTGTTGGC CCCTCTGTTG
    GAAGTCATCA AACATTTCTG TCAAGTAACT AGTGTTCAGA CAGACCTCCT TTCCAGTGCC
    AAACCTCACA AGGAACAGGA GAAAAGTCAG GAGATGGAAA AGAAAGACTG CTCACTCCCC
    CAGTCTGTGG CCGTCACATA CACTCTATTC CAGGAAAAGA AAATGCCCTT ACACAGCATA
    GCTGAGAACA GGCTCCTGCC TCTCACAGCA GCCGGCATGC ACTTAGCCCA GGCGGTGAAA
    GCCGGCTACC CCCTGGATAT GGAGCGAGCT GGCCTGACCC CAGAGACTTG GAAGATTATT
    ATGGATGTCA TCCGAAACCC TCCCATCAAC TCAGATATGT ATAAAGTTAA ACTCATCAGA
    ATGTTAGTTC CTGAAAACTT AGACACGTAC CTCATCCACA TGGCGATTGA GATTCTTCAG
    AGTGGTTCCG ACAGCAGAAC CCAGCCTCCT TGTGATTCCA GCAGGAAGAG GCGTTTCCCC
    AGCTCTGCAG AGAGTTGTGA GAGCTGTAAG GAGAGCAAAG AGGCGGTCAC CGAGACCAAG
    GCATCATCTT CAGAGTCAAA GAGAAAATTA CCCGAGTGGT TTGCCAAAGG AAATGTGCCC
    TCAGCTGATA CCGGCAGCTC ATCATCAATG GCCAAGACCA AAAAGAAAGG TCTCTTTAGT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006509156.1
    CDS230..4432
    Translation

    Target ORF information:

    RefSeq Version XM_006509093.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus Werner syndrome RecQ like helicase (Wrn), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006509093.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGAAACCA CTTCACTACA GCGGAAATTT CCAGAATGGA TGTCTATGCA GAGTCAAAGA 
    TGTGCTACAG AAGAAAAGGC CTGCGTTCAG AAGAGTGTTC TTGAAGATAA CCTCCCATTC
    TTAGAATTCC CTGGATCCAT TGTTTACAGT TATGAAGCTA GTGATTGCTC CTTCCTGTCT
    GAAGACATTA GCATGCGTCT GTCTGATGGC GATGTGGTGG GATTTGACAT GGAATGGCCG
    CCCATATACA AGCCAGGGAA AAGAAGCAGA GTCGCAGTGA TCCAGTTGTG TGTGTCTGAG
    AGCAAATGTT ACTTGTTTCA CATTTCTTCC ATGTCAGTTT TCCCCCAGGG ATTAAAAATG
    TTACTAGAAA ACAAATCAAT TAAGAAGGCA GGGGTTGGGA TTGAAGGGGA CCAGTGGAAA
    CTTCTGCGTG ATTTTGACGT CAAGTTGGAG AGTTTTGTGG AGCTGACGGA TGTTGCCAAT
    GAAAAGTTGA AGTGCGCAGA GACCTGGAGC CTCAATGGTC TGGTTAAACA CGTCTTAGGG
    AAACAACTTT TGAAAGACAA GTCCATCCGC TGCAGCAATT GGAGTAATTT CCCCCTCACT
    GAGGACCAGA AACTGTATGC AGCCACTGAT GCTTATGCTG GTCTTATCAT CTATCAAAAA
    TTAGGAAATT TGGGTGATAC TGCGCAAGTG TTTGCTCTAA ATAAAGAGGA AAACCTACCT
    CTGGAGATGA AGAAACAGTT GAATTCAATC TCCGAAGAAA TGAGGGACCT AGCCAATCGT
    TTTCCTGTCA CTTGCAGAAA TTTGGAAACT CTCCAGAGGG TTCCTGTAAT ATTGAAGAGT
    ATTTCAGAAA ATCTCTGTTC ATTGAGAAAA GTGATCTGTG GTCCTACAAA CACTGAGACT
    AGACTGAAGC CGGGCAGTAG TTTTAATTTA CTGTCATCAG AGGATTCAGC TGCTGCTGGA
    GAAAAAGAGA AACAGATTGG AAAACATAGT ACTTTTGCTA AAATTAAAGA AGAACCATGG
    GACCCAGAAC TTGACAGTTT AGTGAAGCAA GAGGAGGTTG ATGTATTTAG AAATCAAGTG
    AAGCAAGAAA AAGGTGAATC TGAAAATGAA ATAGAAGACA ATCTGTTGAG AGAAGATATG
    GAAAGAACTT GTGTGATTCC TAGTATTTCA GAAAATGAAC TCCAAGATTT GGAACAGCAA
    GCTAAAGAAG AAAAATATAA TGATGTTTCT CACCAACTTT CTGAGCATTT ATCTCCCAAT
    GATGATGAGA ATGACTCCTC CTATATAATT GAAAGTGATG AAGATTTGGA AATGGAGATG
    CTGAAGTCTT TAGAAAACCT AAATAGTGAC GTGGTGGAAC CCACTCACTC TACATGGTTG
    GAAATGGGAA CCAATGGGCG TCTTCCTCCT GAGGAGGAAG ATGGACACGG AAATGAAGCC
    ATCAAAGAGG AGCAGGAAGA AGAGGACCAT TTATTGCCGG AACCCAACGC AAAGCAAATT
    AATTGCCTCA AGACCTATTT CGGACACAGC AGTTTTAAAC CGGTTCAGTG GAAAGTCATC
    CATTCTGTAT TAGAAGAGAG AAGAGATAAT GTTGTTGTCA TGGCAACTGG ATATGGGAAG
    AGTCTGTGCT TCCAGTATCC GCCTGTTTAT ACAGGCAAGA TTGGCATTGT CATTTCACCT
    CTCATTTCCT TAATGGAAGA CCAAGTCCTC CAGCTTGAGC TGTCCAATGT TCCAGCCTGT
    TTACTTGGAT CTGCACAGTC AAAAAATATT CTAGGAGATG TTAAATTAGG CAAATATAGG
    GTCATCTACA TAACTCCAGA GTTCTGTTCT GGTAACTTGG ATCTACTCCA GCAACTTGAC
    TCTAGTATTG GCATCACTCT CATTGCTGTG GATGAGGCTC ACTGCATTTC AGAGTGGGGC
    CATGATTTCA GAAGTTCATT CAGGATGCTG GGCTCTCTTA AAACAGCGCT CCCATTGGTT
    CCAGTCATTG CACTCTCCGC TACTGCAAGC TCTTCCATCC GGGAAGACAT TATAAGCTGC
    TTAAACCTGA AAGACCCTCA GATCACCTGC ACTGGATTTG ATCGGCCAAA TCTGTACTTA
    GAAGTTGGAC GGAAAACAGG GAACATCCTT CAGGATCTAA AGCCGTTTCT CGTCCGAAAG
    GCAAGTTCTG CCTGGGAATT TGAAGGTCCA ACCATCATCT ATTGTCCTTC GAGAAAAATG
    ACAGAACAAG TTACTGCTGA ACTTGGGAAA CTGAACTTAG CCTGCAGAAC ATACCACGCT
    GGCATGAAAA TTAGCGAAAG GAAGGACGTT CATCATAGGT TCCTGAGAGA TGAAATTCAG
    TGTGTTGTAG CTACTGTAGC TTTTGGAATG GGCATTAATA AAGCTGACAT TCGCAAAGTT
    ATTCATTATG GTGCGCCTAA GGAAATGGAA TCCTATTACC AGGAAATTGG TAGAGCTGGC
    CGGGATGGAC TTCAGAGTTC CTGTCACTTG CTCTGGGCTC CAGCAGACTT TAACACATCC
    AGGAATCTCC TTATTGAGAT TCACGATGAA AAGTTCCGGT TATATAAATT AAAGATGATG
    GTAAAGATGG AAAAATACCT TCACTCCAGT CAGTGTAGGC GACGAATCAT CTTGTCCCAT
    TTTGAGGACA AATGTCTGCA GAAGGCCTCC TTGGACATTA TGGGAACTGA AAAATGCTGT
    GATAATTGCA GGCCCAGGCT GAATCATTGC CTTACTGCTA ACAACTCAGA GGACGCATCC
    CAAGACTTTG GGCCACAAGC ATTCCAGCTA CTGTCTGCTG TGGACATCCT GCAGGAGAAA
    TTTGGAATTG GGATTCCGAT CTTATTTCTC CGAGGATCTA ATTCTCAGCG TCTTCCTGAT
    AAATATCGGG GTCACAGGCT CTTTGGTGCT GGAAAGGAGC AAGCAGAAAG TTGGTGGAAG
    ACCCTTTCTC ACCATCTCAT AGCTGAAGGA TTCTTGGTAG AAGTTCCCAA GGAAAACAAA
    TATATAAAGA CATGTTCCCT CACAAAAAAG GGTAGAAAGT GGCTTGGAGA AGCCAGTTCG
    CAGTCTCCTC CGAGCCTTCT CCTTCAAGCT AATGAAGAGA TGTTTCCAAG GAAAGTTCTG
    CTACCAAGTT CTAATCCTGT ATCTCCAGAA ACGACGCAAC ATTCCTCTAA TCAAAACCCA
    GCTGGATTAA CTACCAAGCA GTCTAATTTG GAGAGAACGC ATTCTTACAA AGTGCCTGAG
    AAAGTTTCTT CTGGGACTAA CATTCCTAAA AAAAGTGCCG TGATGCCGTC ACCAGGAACA
    TCTTCCAGCC CCTTAGAACC TGCCATCTCA GCCCAAGAGC TGGACGCTCG GACTGGGCTA
    TATGCCAGGC TGGTGGAAGC AAGGCAGAAA CACGCTAATA AGATGGATGT ACCTCCAGCT
    ATTTTAGCAA CAAACAAGGT TCTGCTGGAC ATGGCTAAAA TGAGACCGAC TACTGTTGAA
    AACATGAAAC AGATCGACGG TGTCTCTGAA GGCAAAGCTG CTCTGTTGGC CCCTCTGTTG
    GAAGTCATCA AACATTTCTG TCAAGTAACT AGTGTTCAGA CAGACCTCCT TTCCAGTGCC
    AAACCTCACA AGGAACAGGA GAAAAGTCAG GAGATGGAAA AGAAAGACTG CTCACTCCCC
    CAGTCTGTGG CCGTCACATA CACTCTATTC CAGGAAAAGA AAATGCCCTT ACACAGCATA
    GCTGAGAACA GGCTCCTGCC TCTCACAGCA GCCGGCATGC ACTTAGCCCA GGCGGTGAAA
    GCCGGCTACC CCCTGGATAT GGAGCGAGCT GGCCTGACCC CAGAGACTTG GAAGATTATT
    ATGGATGTCA TCCGAAACCC TCCCATCAAC TCAGATATGT ATAAAGTTAA ACTCATCAGA
    ATGTTAGTTC CTGAAAACTT AGACACGTAC CTCATCCACA TGGCGATTGA GATTCTTCAG
    AGTGGTTCCG ACAGCAGAAC CCAGCCTCCT TGTGATTCCA GCAGGAAGAG GCGTTTCCCC
    AGCTCTGCAG AGAGTTGTGA GAGCTGTAAG GAGAGCAAAG AGGCGGTCAC CGAGACCAAG
    GCATCATCTT CAGAGTCAAA GAGAAAATTA CCCGAGTGGT TTGCCAAAGG AAATGTGCCC
    TCAGCTGATA CCGGCAGCTC ATCATCAATG GCCAAGACCA AAAAGAAAGG TCTCTTTAGT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu40624
    Clone ID Related Accession (Same CDS sequence) XM_006509093.3 , XM_006509093.4
    Accession Version XM_006509093.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4203bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product Werner syndrome ATP-dependent helicase homolog isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006509093.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGAAACCA CTTCACTACA GCGGAAATTT CCAGAATGGA TGTCTATGCA GAGTCAAAGA 
    TGTGCTACAG AAGAAAAGGC CTGCGTTCAG AAGAGTGTTC TTGAAGATAA CCTCCCATTC
    TTAGAATTCC CTGGATCCAT TGTTTACAGT TATGAAGCTA GTGATTGCTC CTTCCTGTCT
    GAAGACATTA GCATGCGTCT GTCTGATGGC GATGTGGTGG GATTTGACAT GGAATGGCCG
    CCCATATACA AGCCAGGGAA AAGAAGCAGA GTCGCAGTGA TCCAGTTGTG TGTGTCTGAG
    AGCAAATGTT ACTTGTTTCA CATTTCTTCC ATGTCAGTTT TCCCCCAGGG ATTAAAAATG
    TTACTAGAAA ACAAATCAAT TAAGAAGGCA GGGGTTGGGA TTGAAGGGGA CCAGTGGAAA
    CTTCTGCGTG ATTTTGACGT CAAGTTGGAG AGTTTTGTGG AGCTGACGGA TGTTGCCAAT
    GAAAAGTTGA AGTGCGCAGA GACCTGGAGC CTCAATGGTC TGGTTAAACA CGTCTTAGGG
    AAACAACTTT TGAAAGACAA GTCCATCCGC TGCAGCAATT GGAGTAATTT CCCCCTCACT
    GAGGACCAGA AACTGTATGC AGCCACTGAT GCTTATGCTG GTCTTATCAT CTATCAAAAA
    TTAGGAAATT TGGGTGATAC TGCGCAAGTG TTTGCTCTAA ATAAAGAGGA AAACCTACCT
    CTGGAGATGA AGAAACAGTT GAATTCAATC TCCGAAGAAA TGAGGGACCT AGCCAATCGT
    TTTCCTGTCA CTTGCAGAAA TTTGGAAACT CTCCAGAGGG TTCCTGTAAT ATTGAAGAGT
    ATTTCAGAAA ATCTCTGTTC ATTGAGAAAA GTGATCTGTG GTCCTACAAA CACTGAGACT
    AGACTGAAGC CGGGCAGTAG TTTTAATTTA CTGTCATCAG AGGATTCAGC TGCTGCTGGA
    GAAAAAGAGA AACAGATTGG AAAACATAGT ACTTTTGCTA AAATTAAAGA AGAACCATGG
    GACCCAGAAC TTGACAGTTT AGTGAAGCAA GAGGAGGTTG ATGTATTTAG AAATCAAGTG
    AAGCAAGAAA AAGGTGAATC TGAAAATGAA ATAGAAGACA ATCTGTTGAG AGAAGATATG
    GAAAGAACTT GTGTGATTCC TAGTATTTCA GAAAATGAAC TCCAAGATTT GGAACAGCAA
    GCTAAAGAAG AAAAATATAA TGATGTTTCT CACCAACTTT CTGAGCATTT ATCTCCCAAT
    GATGATGAGA ATGACTCCTC CTATATAATT GAAAGTGATG AAGATTTGGA AATGGAGATG
    CTGAAGTCTT TAGAAAACCT AAATAGTGAC GTGGTGGAAC CCACTCACTC TACATGGTTG
    GAAATGGGAA CCAATGGGCG TCTTCCTCCT GAGGAGGAAG ATGGACACGG AAATGAAGCC
    ATCAAAGAGG AGCAGGAAGA AGAGGACCAT TTATTGCCGG AACCCAACGC AAAGCAAATT
    AATTGCCTCA AGACCTATTT CGGACACAGC AGTTTTAAAC CGGTTCAGTG GAAAGTCATC
    CATTCTGTAT TAGAAGAGAG AAGAGATAAT GTTGTTGTCA TGGCAACTGG ATATGGGAAG
    AGTCTGTGCT TCCAGTATCC GCCTGTTTAT ACAGGCAAGA TTGGCATTGT CATTTCACCT
    CTCATTTCCT TAATGGAAGA CCAAGTCCTC CAGCTTGAGC TGTCCAATGT TCCAGCCTGT
    TTACTTGGAT CTGCACAGTC AAAAAATATT CTAGGAGATG TTAAATTAGG CAAATATAGG
    GTCATCTACA TAACTCCAGA GTTCTGTTCT GGTAACTTGG ATCTACTCCA GCAACTTGAC
    TCTAGTATTG GCATCACTCT CATTGCTGTG GATGAGGCTC ACTGCATTTC AGAGTGGGGC
    CATGATTTCA GAAGTTCATT CAGGATGCTG GGCTCTCTTA AAACAGCGCT CCCATTGGTT
    CCAGTCATTG CACTCTCCGC TACTGCAAGC TCTTCCATCC GGGAAGACAT TATAAGCTGC
    TTAAACCTGA AAGACCCTCA GATCACCTGC ACTGGATTTG ATCGGCCAAA TCTGTACTTA
    GAAGTTGGAC GGAAAACAGG GAACATCCTT CAGGATCTAA AGCCGTTTCT CGTCCGAAAG
    GCAAGTTCTG CCTGGGAATT TGAAGGTCCA ACCATCATCT ATTGTCCTTC GAGAAAAATG
    ACAGAACAAG TTACTGCTGA ACTTGGGAAA CTGAACTTAG CCTGCAGAAC ATACCACGCT
    GGCATGAAAA TTAGCGAAAG GAAGGACGTT CATCATAGGT TCCTGAGAGA TGAAATTCAG
    TGTGTTGTAG CTACTGTAGC TTTTGGAATG GGCATTAATA AAGCTGACAT TCGCAAAGTT
    ATTCATTATG GTGCGCCTAA GGAAATGGAA TCCTATTACC AGGAAATTGG TAGAGCTGGC
    CGGGATGGAC TTCAGAGTTC CTGTCACTTG CTCTGGGCTC CAGCAGACTT TAACACATCC
    AGGAATCTCC TTATTGAGAT TCACGATGAA AAGTTCCGGT TATATAAATT AAAGATGATG
    GTAAAGATGG AAAAATACCT TCACTCCAGT CAGTGTAGGC GACGAATCAT CTTGTCCCAT
    TTTGAGGACA AATGTCTGCA GAAGGCCTCC TTGGACATTA TGGGAACTGA AAAATGCTGT
    GATAATTGCA GGCCCAGGCT GAATCATTGC CTTACTGCTA ACAACTCAGA GGACGCATCC
    CAAGACTTTG GGCCACAAGC ATTCCAGCTA CTGTCTGCTG TGGACATCCT GCAGGAGAAA
    TTTGGAATTG GGATTCCGAT CTTATTTCTC CGAGGATCTA ATTCTCAGCG TCTTCCTGAT
    AAATATCGGG GTCACAGGCT CTTTGGTGCT GGAAAGGAGC AAGCAGAAAG TTGGTGGAAG
    ACCCTTTCTC ACCATCTCAT AGCTGAAGGA TTCTTGGTAG AAGTTCCCAA GGAAAACAAA
    TATATAAAGA CATGTTCCCT CACAAAAAAG GGTAGAAAGT GGCTTGGAGA AGCCAGTTCG
    CAGTCTCCTC CGAGCCTTCT CCTTCAAGCT AATGAAGAGA TGTTTCCAAG GAAAGTTCTG
    CTACCAAGTT CTAATCCTGT ATCTCCAGAA ACGACGCAAC ATTCCTCTAA TCAAAACCCA
    GCTGGATTAA CTACCAAGCA GTCTAATTTG GAGAGAACGC ATTCTTACAA AGTGCCTGAG
    AAAGTTTCTT CTGGGACTAA CATTCCTAAA AAAAGTGCCG TGATGCCGTC ACCAGGAACA
    TCTTCCAGCC CCTTAGAACC TGCCATCTCA GCCCAAGAGC TGGACGCTCG GACTGGGCTA
    TATGCCAGGC TGGTGGAAGC AAGGCAGAAA CACGCTAATA AGATGGATGT ACCTCCAGCT
    ATTTTAGCAA CAAACAAGGT TCTGCTGGAC ATGGCTAAAA TGAGACCGAC TACTGTTGAA
    AACATGAAAC AGATCGACGG TGTCTCTGAA GGCAAAGCTG CTCTGTTGGC CCCTCTGTTG
    GAAGTCATCA AACATTTCTG TCAAGTAACT AGTGTTCAGA CAGACCTCCT TTCCAGTGCC
    AAACCTCACA AGGAACAGGA GAAAAGTCAG GAGATGGAAA AGAAAGACTG CTCACTCCCC
    CAGTCTGTGG CCGTCACATA CACTCTATTC CAGGAAAAGA AAATGCCCTT ACACAGCATA
    GCTGAGAACA GGCTCCTGCC TCTCACAGCA GCCGGCATGC ACTTAGCCCA GGCGGTGAAA
    GCCGGCTACC CCCTGGATAT GGAGCGAGCT GGCCTGACCC CAGAGACTTG GAAGATTATT
    ATGGATGTCA TCCGAAACCC TCCCATCAAC TCAGATATGT ATAAAGTTAA ACTCATCAGA
    ATGTTAGTTC CTGAAAACTT AGACACGTAC CTCATCCACA TGGCGATTGA GATTCTTCAG
    AGTGGTTCCG ACAGCAGAAC CCAGCCTCCT TGTGATTCCA GCAGGAAGAG GCGTTTCCCC
    AGCTCTGCAG AGAGTTGTGA GAGCTGTAAG GAGAGCAAAG AGGCGGTCAC CGAGACCAAG
    GCATCATCTT CAGAGTCAAA GAGAAAATTA CCCGAGTGGT TTGCCAAAGG AAATGTGCCC
    TCAGCTGATA CCGGCAGCTC ATCATCAATG GCCAAGACCA AAAAGAAAGG TCTCTTTAGT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006509156.1
    CDS229..4431
    Translation

    Target ORF information:

    RefSeq Version XM_006509093.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus Werner syndrome homolog (human) (Wrn), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006509093.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGAAACCA CTTCACTACA GCGGAAATTT CCAGAATGGA TGTCTATGCA GAGTCAAAGA 
    TGTGCTACAG AAGAAAAGGC CTGCGTTCAG AAGAGTGTTC TTGAAGATAA CCTCCCATTC
    TTAGAATTCC CTGGATCCAT TGTTTACAGT TATGAAGCTA GTGATTGCTC CTTCCTGTCT
    GAAGACATTA GCATGCGTCT GTCTGATGGC GATGTGGTGG GATTTGACAT GGAATGGCCG
    CCCATATACA AGCCAGGGAA AAGAAGCAGA GTCGCAGTGA TCCAGTTGTG TGTGTCTGAG
    AGCAAATGTT ACTTGTTTCA CATTTCTTCC ATGTCAGTTT TCCCCCAGGG ATTAAAAATG
    TTACTAGAAA ACAAATCAAT TAAGAAGGCA GGGGTTGGGA TTGAAGGGGA CCAGTGGAAA
    CTTCTGCGTG ATTTTGACGT CAAGTTGGAG AGTTTTGTGG AGCTGACGGA TGTTGCCAAT
    GAAAAGTTGA AGTGCGCAGA GACCTGGAGC CTCAATGGTC TGGTTAAACA CGTCTTAGGG
    AAACAACTTT TGAAAGACAA GTCCATCCGC TGCAGCAATT GGAGTAATTT CCCCCTCACT
    GAGGACCAGA AACTGTATGC AGCCACTGAT GCTTATGCTG GTCTTATCAT CTATCAAAAA
    TTAGGAAATT TGGGTGATAC TGCGCAAGTG TTTGCTCTAA ATAAAGAGGA AAACCTACCT
    CTGGAGATGA AGAAACAGTT GAATTCAATC TCCGAAGAAA TGAGGGACCT AGCCAATCGT
    TTTCCTGTCA CTTGCAGAAA TTTGGAAACT CTCCAGAGGG TTCCTGTAAT ATTGAAGAGT
    ATTTCAGAAA ATCTCTGTTC ATTGAGAAAA GTGATCTGTG GTCCTACAAA CACTGAGACT
    AGACTGAAGC CGGGCAGTAG TTTTAATTTA CTGTCATCAG AGGATTCAGC TGCTGCTGGA
    GAAAAAGAGA AACAGATTGG AAAACATAGT ACTTTTGCTA AAATTAAAGA AGAACCATGG
    GACCCAGAAC TTGACAGTTT AGTGAAGCAA GAGGAGGTTG ATGTATTTAG AAATCAAGTG
    AAGCAAGAAA AAGGTGAATC TGAAAATGAA ATAGAAGACA ATCTGTTGAG AGAAGATATG
    GAAAGAACTT GTGTGATTCC TAGTATTTCA GAAAATGAAC TCCAAGATTT GGAACAGCAA
    GCTAAAGAAG AAAAATATAA TGATGTTTCT CACCAACTTT CTGAGCATTT ATCTCCCAAT
    GATGATGAGA ATGACTCCTC CTATATAATT GAAAGTGATG AAGATTTGGA AATGGAGATG
    CTGAAGTCTT TAGAAAACCT AAATAGTGAC GTGGTGGAAC CCACTCACTC TACATGGTTG
    GAAATGGGAA CCAATGGGCG TCTTCCTCCT GAGGAGGAAG ATGGACACGG AAATGAAGCC
    ATCAAAGAGG AGCAGGAAGA AGAGGACCAT TTATTGCCGG AACCCAACGC AAAGCAAATT
    AATTGCCTCA AGACCTATTT CGGACACAGC AGTTTTAAAC CGGTTCAGTG GAAAGTCATC
    CATTCTGTAT TAGAAGAGAG AAGAGATAAT GTTGTTGTCA TGGCAACTGG ATATGGGAAG
    AGTCTGTGCT TCCAGTATCC GCCTGTTTAT ACAGGCAAGA TTGGCATTGT CATTTCACCT
    CTCATTTCCT TAATGGAAGA CCAAGTCCTC CAGCTTGAGC TGTCCAATGT TCCAGCCTGT
    TTACTTGGAT CTGCACAGTC AAAAAATATT CTAGGAGATG TTAAATTAGG CAAATATAGG
    GTCATCTACA TAACTCCAGA GTTCTGTTCT GGTAACTTGG ATCTACTCCA GCAACTTGAC
    TCTAGTATTG GCATCACTCT CATTGCTGTG GATGAGGCTC ACTGCATTTC AGAGTGGGGC
    CATGATTTCA GAAGTTCATT CAGGATGCTG GGCTCTCTTA AAACAGCGCT CCCATTGGTT
    CCAGTCATTG CACTCTCCGC TACTGCAAGC TCTTCCATCC GGGAAGACAT TATAAGCTGC
    TTAAACCTGA AAGACCCTCA GATCACCTGC ACTGGATTTG ATCGGCCAAA TCTGTACTTA
    GAAGTTGGAC GGAAAACAGG GAACATCCTT CAGGATCTAA AGCCGTTTCT CGTCCGAAAG
    GCAAGTTCTG CCTGGGAATT TGAAGGTCCA ACCATCATCT ATTGTCCTTC GAGAAAAATG
    ACAGAACAAG TTACTGCTGA ACTTGGGAAA CTGAACTTAG CCTGCAGAAC ATACCACGCT
    GGCATGAAAA TTAGCGAAAG GAAGGACGTT CATCATAGGT TCCTGAGAGA TGAAATTCAG
    TGTGTTGTAG CTACTGTAGC TTTTGGAATG GGCATTAATA AAGCTGACAT TCGCAAAGTT
    ATTCATTATG GTGCGCCTAA GGAAATGGAA TCCTATTACC AGGAAATTGG TAGAGCTGGC
    CGGGATGGAC TTCAGAGTTC CTGTCACTTG CTCTGGGCTC CAGCAGACTT TAACACATCC
    AGGAATCTCC TTATTGAGAT TCACGATGAA AAGTTCCGGT TATATAAATT AAAGATGATG
    GTAAAGATGG AAAAATACCT TCACTCCAGT CAGTGTAGGC GACGAATCAT CTTGTCCCAT
    TTTGAGGACA AATGTCTGCA GAAGGCCTCC TTGGACATTA TGGGAACTGA AAAATGCTGT
    GATAATTGCA GGCCCAGGCT GAATCATTGC CTTACTGCTA ACAACTCAGA GGACGCATCC
    CAAGACTTTG GGCCACAAGC ATTCCAGCTA CTGTCTGCTG TGGACATCCT GCAGGAGAAA
    TTTGGAATTG GGATTCCGAT CTTATTTCTC CGAGGATCTA ATTCTCAGCG TCTTCCTGAT
    AAATATCGGG GTCACAGGCT CTTTGGTGCT GGAAAGGAGC AAGCAGAAAG TTGGTGGAAG
    ACCCTTTCTC ACCATCTCAT AGCTGAAGGA TTCTTGGTAG AAGTTCCCAA GGAAAACAAA
    TATATAAAGA CATGTTCCCT CACAAAAAAG GGTAGAAAGT GGCTTGGAGA AGCCAGTTCG
    CAGTCTCCTC CGAGCCTTCT CCTTCAAGCT AATGAAGAGA TGTTTCCAAG GAAAGTTCTG
    CTACCAAGTT CTAATCCTGT ATCTCCAGAA ACGACGCAAC ATTCCTCTAA TCAAAACCCA
    GCTGGATTAA CTACCAAGCA GTCTAATTTG GAGAGAACGC ATTCTTACAA AGTGCCTGAG
    AAAGTTTCTT CTGGGACTAA CATTCCTAAA AAAAGTGCCG TGATGCCGTC ACCAGGAACA
    TCTTCCAGCC CCTTAGAACC TGCCATCTCA GCCCAAGAGC TGGACGCTCG GACTGGGCTA
    TATGCCAGGC TGGTGGAAGC AAGGCAGAAA CACGCTAATA AGATGGATGT ACCTCCAGCT
    ATTTTAGCAA CAAACAAGGT TCTGCTGGAC ATGGCTAAAA TGAGACCGAC TACTGTTGAA
    AACATGAAAC AGATCGACGG TGTCTCTGAA GGCAAAGCTG CTCTGTTGGC CCCTCTGTTG
    GAAGTCATCA AACATTTCTG TCAAGTAACT AGTGTTCAGA CAGACCTCCT TTCCAGTGCC
    AAACCTCACA AGGAACAGGA GAAAAGTCAG GAGATGGAAA AGAAAGACTG CTCACTCCCC
    CAGTCTGTGG CCGTCACATA CACTCTATTC CAGGAAAAGA AAATGCCCTT ACACAGCATA
    GCTGAGAACA GGCTCCTGCC TCTCACAGCA GCCGGCATGC ACTTAGCCCA GGCGGTGAAA
    GCCGGCTACC CCCTGGATAT GGAGCGAGCT GGCCTGACCC CAGAGACTTG GAAGATTATT
    ATGGATGTCA TCCGAAACCC TCCCATCAAC TCAGATATGT ATAAAGTTAA ACTCATCAGA
    ATGTTAGTTC CTGAAAACTT AGACACGTAC CTCATCCACA TGGCGATTGA GATTCTTCAG
    AGTGGTTCCG ACAGCAGAAC CCAGCCTCCT TGTGATTCCA GCAGGAAGAG GCGTTTCCCC
    AGCTCTGCAG AGAGTTGTGA GAGCTGTAAG GAGAGCAAAG AGGCGGTCAC CGAGACCAAG
    GCATCATCTT CAGAGTCAAA GAGAAAATTA CCCGAGTGGT TTGCCAAAGG AAATGTGCCC
    TCAGCTGATA CCGGCAGCTC ATCATCAATG GCCAAGACCA AAAAGAAAGG TCTCTTTAGT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.