Muc20 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Muc20
Entrez Gene ID	224116
Full Name	mucin 20
Synonyms	BC026367
General protein information	Preferred Names mucin 20 Names mucin-20 MUC-20
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 16 Map Location: 16\|16 B3

mRNA and Protein(s)

mRNA	Protein	Name
NM_146071.2	NP_666183.2	mucin-20 isoform b precursor
NM_001145874.1	NP_001139346.1	mucin-20 isoform a precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	MUC20	NP_001269435.1
Pan troglodytes (chimpanzee)	LOC750356	XP_001173992.3
Canis lupus familiaris (dog)	MUC20	XP_545146.3
Rattus norvegicus (Norway rat)	Muc20	NP_001100559.1
	LOC713400	XP_001102737.2
Mus musculus (house mouse)	Muc20	NP_666183.2

Gene Ontology
Bibliography

Related articles in PubMed

The Interleukin-2-mTORc1 Kinase Axis Defines the Signaling, Differentiation, and Metabolism of T Helper 1 and Follicular B Helper T Cells.
Ray JP, Staron MM, Shyer JA, Ho PC, Marshall HD, Gray SM, Laidlaw BJ, Araki K, Ahmed R, Kaech SM, Craft J
Immunity43(4)690-702(2015 Oct)

A mouse knockout library for secreted and transmembrane proteins.
Tang T, Li L, Tang J, Li Y, Lin WY, Martin F, Grant D, Solloway M, Parker L, Ye W, Forrest W, Ghilardi N, Oravecz T, Platt KA, Rice DS, Hansen GM, Abuin A, Eberhart DE, Godowski P, Holt KH, Peterson A, Zambrowicz BP, de Sauvage FJ
Nature biotechnology28(7)749-55(2010 Jul)

MUC20 suppresses the hepatocyte growth factor-induced Grb2-Ras pathway by binding to a multifunctional docking site of met.
Higuchi T, Orita T, Katsuya K, Yamasaki Y, Akiyama K, Li H, Yamamoto T, Saito Y, Nakamura M
Molecular and cellular biology24(17)7456-68(2004 Sep)

Molecular cloning, genomic structure, and expression analysis of MUC20, a novel mucin protein, up-regulated in injured kidney.
Higuchi T, Orita T, Nakanishi S, Katsuya K, Watanabe H, Yamasaki Y, Waga I, Nanayama T, Yamamoto Y, Munger W, Sun HW, Falk RJ, Jennette JC, Alcorta DA, Li H, Yamamoto T, Saito Y, Nakamura M
The Journal of biological chemistry279(3)1968-79(2004 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results suggest that MUC20 is a novel regulator of the Met signaling cascade which has a role in suppression of the Grb2-Ras pathway.
Title: MUC20 suppresses the hepatocyte growth factor-induced Grb2-Ras pathway by binding to a multifunctional docking site of met.

The following Muc20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Muc20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu07006	NM_001145874.1 Latest version!	Mus musculus mucin 20 (Muc20), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OMu12189	NM_146071.2 Latest version!	Mus musculus mucin 20 (Muc20), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Muc20 ( NM_001145874.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001139346.1 Mus musculus mucin 20 (Muc20), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu07006
Clone ID Related Accession (Same CDS sequence)	NM_001145874.1
Accession Version	NM_001145874.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	mucin-20 isoform a precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK142548.1, AK165477.1, BB615450.1 and AC139244.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK142548.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849381 [ECO:0000348] ##Evidence-Data-END##

ATGGGCTCTG TCTGGGGCCT GGCTGTGCCC CTCCTTGTCT TCTGCTGGAA GGTTGGCGTC 
AGTGTGAGCT CTCCAGGTCT CGACATTAGC AGATCAGTTC CTTTGGTGAC AACCAACAAC 
ATGGAAGTGT CCACCTTCAC TCAAAGGGAC AGGCCCAGCT CAGAGAGAGC TTTCCAGACC 
ACCAACCTGA TTCAGTATGT ACCTCTAGAC ACGCAAACTC TCAGCACTGA ATCTGCTTCT 
AATGCTTTAA GTGCAACTAG TATCAGTTCA GAGGTCAATT CCAGAGACAC TCAGACCACC 
TCTTTTGTAA CAAAGACCAG GAAGACACAT ACTACCACTC CTGCAGCATC ATCCCTAGAG 
GCCCAGACCA CCTCTCCTAA TTTGTCAACC CTGAATATTC AGATTACCTC CCCAATAGCA 
TCGTCCCTGG ATGCTCAGAC CATTTTTCCT GTATCATTAT CCCTGAGTAC CCAGACCACT 
TCTCCTGCAC CATCATTCCT GGATACCCAA ACCACTTCCC CTGAACCATC ATCTCTGACC 
ACTTCCCCTG CACCATCATC TCTGATCACT TCTCCTACTC CATCATCTCT AACTACATCC 
CCTGCACCAT CATTCCTGGA TACCCAGACC ACTTCCCCTG CACCATCATC TCTGACCACT 
TCCCCTGCAC CATCATCCCT AGATACCCAG ACCATCTCAC CTATTGAATT GACCCTAAAG 
ACCCAAACCA TTTCCACTGT AACGGAGACC AGAACGGTTT CAATAAGAAT ACCTTCTGAC 
CTCACGGTTA TGCACACCAT CCCTACAGAG ACATTGGCAC CAAGCAACAG CCCCAGAACT 
GGGATGAGCA CTGTCCAAAC TGGCACAGTG TGGGACTCCA TAGAAGCCGT CTTTGACACC 
CTTTGTACCG ATGACAGCTC TGAAGAGGCA AGGAAGATCA CAGTTGACCT CCTGACGTTG 
GCACACACCT CCACGGAAGT GGAGTACCTG TCTTCAGAGA GCAGCTCTTC CTCTGACAGT 
TCAGCTGGGG TCCTCAGCAG CTCACGAGTC CTGGGACCCG ACAGTGCAAC TCCAGCCAAG 
GGGTTGGTTG CCTTCAACAT CACTCATATT AAACTCAGCA ACTGCATCAC AGAAATAGAA 
ACAACCATCA CCATCTCTGG GGCCCCAGGC GCGAGCCTCA GCCCTACAGA AGCGACGGCA 
GCTTTGTTCA CTTCTGAGAT ATTAACTTTG CCTCCACCCA CTGAGGCAAA ACCAATTTTC 
CCCGAGACCA CAAGCTTATC TGGGATCCTG TCAACTGCTG GCACCCCGGC CCTTGCCACC 
ACCCTTGAGG GGACAGTCTC CACTAGTGCA ATCACAGAAA GCGAAACAGC AGTGGCCCAG 
ACTCTTACCT CTGTTGGAAC TTCGGTGACA GTTAGAAGGA ACCCCCTGGA AAACACTTCA 
ACACTCTCTA TTGAGACACA AAGCCACACT GAGGTCTTGG GCACCATCAC AGTCCCCATG 
GTTGCTGGAT CAACAATGGG AGAAGCAGCC TCCTTCGTGA GTTTCACGGC TCTCGACAGC 
AGCAGTCTCT CAGTGGTGGT CACCACCGAG AGCTCTGCTA CCTCAGAGAC TTTAACCACA 
GGCAATACAA CCAACAGCTC CTTCCTCACA GAGAGCCACC CTCCTTTTTC TATCTATTCA 
ACTACAGCTA GCACCAGCAA AAACCCAAAC ATCACCTTAA CCAAGACCAC AGCCTCACCA 
AAGCCCCCGA CGCATCCTAC AACATCTGCA TCCACTGCTT GGATAAGGAA AACCACAAAA 
CATGATCCAG GTGAAGATGG AGGCTTCCTC CTTGTACGGC TGACCGTGGC CTCCCCTAAG 
GACCTCACTG AGCACAACGC CAGAGAGAAG CTGATGAACC AGATAGTGAC TCACGAGCCA 
TCTGGTCCTG GACATCTGCC CTCGAGATGG GGAAGAAGTG GACGTCCTTC CTGGCTTCTC 
CACTCCTCTT CTGCCTCTCC TGCCGAGCCT GCAGGAGGAA GGCCTCCATC ACGGCGCCTA 
TCAGCTGGTT GTTCAGATAG TGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001139346.1
CDS	44..2110
Translation	MGSVWGLAVPLLVFCWKVGVSVSSPGLDISRSVPLVTTNNMEVSTFTQRDRPSSERAFQTTNLIQYVPLDTQTLSTESASNALSATSISSEVNSRDTQTTSFVTKTRKTHTTTPAASSLEAQTTSPNLSTLNIQITSPIASSLDAQTIFPVSLSLSTQTTSPAPSFLDTQTTSPEPSSLTTSPAPSSLITSPTPSSLTTSPAPSFLDTQTTSPAPSSLTTSPAPSSLDTQTISPIELTLKTQTISTVTETRTVSIRIPSDLTVMHTIPTETLAPSNSPRTGMSTVQTGTVWDSIEAVFDTLCTDDSSEEARKITVDLLTLAHTSTEVEYLSSESSSSSDSSAGVLSSSRVLGPDSATPAKGLVAFNITHIKLSNCITEIETTITISGAPGASLSPTEATAALFTSEILTLPPPTEAKPIFPETTSLSGILSTAGTPALATTLEGTVSTSAITESETAVAQTLTSVGTSVTVRRNPLENTSTLSIETQSHTEVLGTITVPMVAGSTMGEAASFVSFTALDSSSLSVVVTTESSATSETLTTGNTTNSSFLTESHPPFSIYSTTASTSKNPNITLTKTTASPKPPTHPTTSASTAWIRKTTKHDPGEDGGFLLVRLTVASPKDLTEHNAREKLMNQIVTHEPSGPGHLPSRWGRSGRPSWLLHSSSASPAEPAGGRPPSRRLSAGCSDSG

Target ORF information:

RefSeq Version	NM_001145874.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mucin 20 (Muc20), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001145874.1

ORF Insert Sequence:

ATGGGCTCTG TCTGGGGCCT GGCTGTGCCC CTCCTTGTCT TCTGCTGGAA GGTTGGCGTC 
AGTGTGAGCT CTCCAGGTCT CGACATTAGC AGATCAGTTC CTTTGGTGAC AACCAACAAC 
ATGGAAGTGT CCACCTTCAC TCAAAGGGAC AGGCCCAGCT CAGAGAGAGC TTTCCAGACC 
ACCAACCTGA TTCAGTATGT ACCTCTAGAC ACGCAAACTC TCAGCACTGA ATCTGCTTCT 
AATGCTTTAA GTGCAACTAG TATCAGTTCA GAGGTCAATT CCAGAGACAC TCAGACCACC 
TCTTTTGTAA CAAAGACCAG GAAGACACAT ACTACCACTC CTGCAGCATC ATCCCTAGAG 
GCCCAGACCA CCTCTCCTAA TTTGTCAACC CTGAATATTC AGATTACCTC CCCAATAGCA 
TCGTCCCTGG ATGCTCAGAC CATTTTTCCT GTATCATTAT CCCTGAGTAC CCAGACCACT 
TCTCCTGCAC CATCATTCCT GGATACCCAA ACCACTTCCC CTGAACCATC ATCTCTGACC 
ACTTCCCCTG CACCATCATC TCTGATCACT TCTCCTACTC CATCATCTCT AACTACATCC 
CCTGCACCAT CATTCCTGGA TACCCAGACC ACTTCCCCTG CACCATCATC TCTGACCACT 
TCCCCTGCAC CATCATCCCT AGATACCCAG ACCATCTCAC CTATTGAATT GACCCTAAAG 
ACCCAAACCA TTTCCACTGT AACGGAGACC AGAACGGTTT CAATAAGAAT ACCTTCTGAC 
CTCACGGTTA TGCACACCAT CCCTACAGAG ACATTGGCAC CAAGCAACAG CCCCAGAACT 
GGGATGAGCA CTGTCCAAAC TGGCACAGTG TGGGACTCCA TAGAAGCCGT CTTTGACACC 
CTTTGTACCG ATGACAGCTC TGAAGAGGCA AGGAAGATCA CAGTTGACCT CCTGACGTTG 
GCACACACCT CCACGGAAGT GGAGTACCTG TCTTCAGAGA GCAGCTCTTC CTCTGACAGT 
TCAGCTGGGG TCCTCAGCAG CTCACGAGTC CTGGGACCCG ACAGTGCAAC TCCAGCCAAG 
GGGTTGGTTG CCTTCAACAT CACTCATATT AAACTCAGCA ACTGCATCAC AGAAATAGAA 
ACAACCATCA CCATCTCTGG GGCCCCAGGC GCGAGCCTCA GCCCTACAGA AGCGACGGCA 
GCTTTGTTCA CTTCTGAGAT ATTAACTTTG CCTCCACCCA CTGAGGCAAA ACCAATTTTC 
CCCGAGACCA CAAGCTTATC TGGGATCCTG TCAACTGCTG GCACCCCGGC CCTTGCCACC 
ACCCTTGAGG GGACAGTCTC CACTAGTGCA ATCACAGAAA GCGAAACAGC AGTGGCCCAG 
ACTCTTACCT CTGTTGGAAC TTCGGTGACA GTTAGAAGGA ACCCCCTGGA AAACACTTCA 
ACACTCTCTA TTGAGACACA AAGCCACACT GAGGTCTTGG GCACCATCAC AGTCCCCATG 
GTTGCTGGAT CAACAATGGG AGAAGCAGCC TCCTTCGTGA GTTTCACGGC TCTCGACAGC 
AGCAGTCTCT CAGTGGTGGT CACCACCGAG AGCTCTGCTA CCTCAGAGAC TTTAACCACA 
GGCAATACAA CCAACAGCTC CTTCCTCACA GAGAGCCACC CTCCTTTTTC TATCTATTCA 
ACTACAGCTA GCACCAGCAA AAACCCAAAC ATCACCTTAA CCAAGACCAC AGCCTCACCA 
AAGCCCCCGA CGCATCCTAC AACATCTGCA TCCACTGCTT GGATAAGGAA AACCACAAAA 
CATGATCCAG GTGAAGATGG AGGCTTCCTC CTTGTACGGC TGACCGTGGC CTCCCCTAAG 
GACCTCACTG AGCACAACGC CAGAGAGAAG CTGATGAACC AGATAGTGAC TCACGAGCCA 
TCTGGTCCTG GACATCTGCC CTCGAGATGG GGAAGAAGTG GACGTCCTTC CTGGCTTCTC 
CACTCCTCTT CTGCCTCTCC TGCCGAGCCT GCAGGAGGAA GGCCTCCATC ACGGCGCCTA 
TCAGCTGGTT GTTCAGATAG TGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Muc20 ( NM_146071.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_666183.2 Mus musculus mucin 20 (Muc20), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu12189
Clone ID Related Accession (Same CDS sequence)	NM_146071.2
Accession Version	NM_146071.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1971bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	mucin-20 isoform b precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB661244.1 and AK165477.1. On Mar 17, 2009 this sequence version replaced NM_146071.1. Transcript Variant: This variant (2) differs in the 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (b) has a distinct C-terminus and is shorter than isoform a. ##Evidence-Data-START## Transcript exon combination :: AK165477.1, AB098732.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGGCTCTG TCTGGGGCCT GGCTGTGCCC CTCCTTGTCT TCTGCTGGAA GGTTGGCGTC 
AGTGTGAGCT CTCCAGGTCT CGACATTAGC AGATCAGTTC CTTTGGTGAC AACCAACAAC 
ATGGAAGTGT CCACCTTCAC TCAAAGGGAC AGGCCCAGCT CAGAGAGAGC TTTCCAGACC 
ACCAACCTGA TTCAGTATGT ACCTCTAGAC ACGCAAACTC TCAGCACTGA ATCTGCTTCT 
AATGCTTTAA GTGCAACTAG TATCAGTTCA GAGGTCAATT CCAGAGACAC TCAGACCACC 
TCTTTTGTAA CAAAGACCAG GAAGACACAT ACTACCACTC CTGCAGCATC ATCCCTAGAG 
GCCCAGACCA CCTCTCCTAA TTTGTCAACC CTGAATATTC AGATTACCTC CCCAATAGCA 
TCGTCCCTGG ATGCTCAGAC CATTTTTCCT GTATCATTAT CCCTGAGTAC CCAGACCACT 
TCTCCTGCAC CATCATTCCT GGATACCCAA ACCACTTCCC CTGAACCATC ATCTCTGACC 
ACTTCCCCTG CACCATCATC TCTGATCACT TCTCCTACTC CATCATCTCT AACTACATCC 
CCTGCACCAT CATTCCTGGA TACCCAGACC ACTTCCCCTG CACCATCATC TCTGACCACT 
TCCCCTGCAC CATCATCCCT AGATACCCAG ACCATCTCAC CTATTGAATT GACCCTAAAG 
ACCCAAACCA TTTCCACTGT AACGGAGACC AGAACGGTTT CAATAAGAAT ACCTTCTGAC 
CTCACGGTTA TGCACACCAT CCCTACAGAG ACATTGGCAC CAAGCAACAG CCCCAGAACT 
GGGATGAGCA CTGTCCAAAC TGGCACAGTG TGGGACTCCA TAGAAGCCGT CTTTGACACC 
CTTTGTACCG ATGACAGCTC TGAAGAGGCA AGGAAGATCA CAGTTGACCT CCTGACGTTG 
GCACACACCT CCACGGAAGT GGAGTACCTG TCTTCAGAGA GCAGCTCTTC CTCTGACAGT 
TCAGCTGGGG TCCTCAGCAG CTCACGAGTC CTGGGACCCG ACAGTGCAAC TCCAGCCAAG 
GGGTTGGTTG CCTTCAACAT CACTCATATT AAACTCAGCA ACTGCATCAC AGAAATAGAA 
ACAACCATCA CCATCTCTGG GGCCCCAGGC GCGAGCCTCA GCCCTACAGA AGCGACGGCA 
GCTTTGTTCA CTTCTGAGAT ATTAACTTTG CCTCCACCCA CTGAGGCAAA ACCAATTTTC 
CCCGAGACCA CAAGCTTATC TGGGATCCTG TCAACTGCTG GCACCCCGGC CCTTGCCACC 
ACCCTTGAGG GGACAGTCTC CACTAGTGCA ATCACAGAAA GCGAAACAGC AGTGGCCCAG 
ACTCTTACCT CTGTTGGAAC TTCGGTGACA GTTAGAAGGA ACCCCCTGGA AAACACTTCA 
ACACTCTCTA TTGAGACACA AAGCCACACT GAGGTCTTGG GCACCATCAC AGTCCCCATG 
GTTGCTGGAT CAACAATGGG AGAAGCAGCC TCCTTCGTGA GTTTCACGGC TCTCGACAGC 
AGCAGTCTCT CAGTGGTGGT CACCACCGAG AGCTCTGCTA CCTCAGAGAC TTTAACCACA 
GGCAATACAA CCAACAGCTC CTTCCTCACA GAGAGCCACC CTCCTTTTTC TATCTATTCA 
ACTACAGCTA GCACCAGCAA AAACCCAAAC ATCACCTTAA CCAAGACCAC AGCCTCACCA 
AAGCCCCCGA CGCATCCTAC AACATCTGCA TCCACTGCTT GGATAAGGAA AACCACAAAA 
CATGATCCAG GTGAAGATGG AGGCTTCCTC CTTGTACGGC TGACCGTGGC CTCCCCTAAG 
GACCTCACTG AGCACAACGC CAGAGAGAAG CTGATGAACC AGCTCCGCCG TGAACTGCAC 
GCCCGCATGC CACTTGTCCA CATGTCCTTC CTGAGCATCA GGAGGGGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_666183.2
CDS	44..2014
Translation	MGSVWGLAVPLLVFCWKVGVSVSSPGLDISRSVPLVTTNNMEVSTFTQRDRPSSERAFQTTNLIQYVPLDTQTLSTESASNALSATSISSEVNSRDTQTTSFVTKTRKTHTTTPAASSLEAQTTSPNLSTLNIQITSPIASSLDAQTIFPVSLSLSTQTTSPAPSFLDTQTTSPEPSSLTTSPAPSSLITSPTPSSLTTSPAPSFLDTQTTSPAPSSLTTSPAPSSLDTQTISPIELTLKTQTISTVTETRTVSIRIPSDLTVMHTIPTETLAPSNSPRTGMSTVQTGTVWDSIEAVFDTLCTDDSSEEARKITVDLLTLAHTSTEVEYLSSESSSSSDSSAGVLSSSRVLGPDSATPAKGLVAFNITHIKLSNCITEIETTITISGAPGASLSPTEATAALFTSEILTLPPPTEAKPIFPETTSLSGILSTAGTPALATTLEGTVSTSAITESETAVAQTLTSVGTSVTVRRNPLENTSTLSIETQSHTEVLGTITVPMVAGSTMGEAASFVSFTALDSSSLSVVVTTESSATSETLTTGNTTNSSFLTESHPPFSIYSTTASTSKNPNITLTKTTASPKPPTHPTTSASTAWIRKTTKHDPGEDGGFLLVRLTVASPKDLTEHNAREKLMNQLRRELHARMPLVHMSFLSIRRG

Target ORF information:

RefSeq Version	NM_146071.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus mucin 20 (Muc20), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_146071.2

ORF Insert Sequence:

ATGGGCTCTG TCTGGGGCCT GGCTGTGCCC CTCCTTGTCT TCTGCTGGAA GGTTGGCGTC 
AGTGTGAGCT CTCCAGGTCT CGACATTAGC AGATCAGTTC CTTTGGTGAC AACCAACAAC 
ATGGAAGTGT CCACCTTCAC TCAAAGGGAC AGGCCCAGCT CAGAGAGAGC TTTCCAGACC 
ACCAACCTGA TTCAGTATGT ACCTCTAGAC ACGCAAACTC TCAGCACTGA ATCTGCTTCT 
AATGCTTTAA GTGCAACTAG TATCAGTTCA GAGGTCAATT CCAGAGACAC TCAGACCACC 
TCTTTTGTAA CAAAGACCAG GAAGACACAT ACTACCACTC CTGCAGCATC ATCCCTAGAG 
GCCCAGACCA CCTCTCCTAA TTTGTCAACC CTGAATATTC AGATTACCTC CCCAATAGCA 
TCGTCCCTGG ATGCTCAGAC CATTTTTCCT GTATCATTAT CCCTGAGTAC CCAGACCACT 
TCTCCTGCAC CATCATTCCT GGATACCCAA ACCACTTCCC CTGAACCATC ATCTCTGACC 
ACTTCCCCTG CACCATCATC TCTGATCACT TCTCCTACTC CATCATCTCT AACTACATCC 
CCTGCACCAT CATTCCTGGA TACCCAGACC ACTTCCCCTG CACCATCATC TCTGACCACT 
TCCCCTGCAC CATCATCCCT AGATACCCAG ACCATCTCAC CTATTGAATT GACCCTAAAG 
ACCCAAACCA TTTCCACTGT AACGGAGACC AGAACGGTTT CAATAAGAAT ACCTTCTGAC 
CTCACGGTTA TGCACACCAT CCCTACAGAG ACATTGGCAC CAAGCAACAG CCCCAGAACT 
GGGATGAGCA CTGTCCAAAC TGGCACAGTG TGGGACTCCA TAGAAGCCGT CTTTGACACC 
CTTTGTACCG ATGACAGCTC TGAAGAGGCA AGGAAGATCA CAGTTGACCT CCTGACGTTG 
GCACACACCT CCACGGAAGT GGAGTACCTG TCTTCAGAGA GCAGCTCTTC CTCTGACAGT 
TCAGCTGGGG TCCTCAGCAG CTCACGAGTC CTGGGACCCG ACAGTGCAAC TCCAGCCAAG 
GGGTTGGTTG CCTTCAACAT CACTCATATT AAACTCAGCA ACTGCATCAC AGAAATAGAA 
ACAACCATCA CCATCTCTGG GGCCCCAGGC GCGAGCCTCA GCCCTACAGA AGCGACGGCA 
GCTTTGTTCA CTTCTGAGAT ATTAACTTTG CCTCCACCCA CTGAGGCAAA ACCAATTTTC 
CCCGAGACCA CAAGCTTATC TGGGATCCTG TCAACTGCTG GCACCCCGGC CCTTGCCACC 
ACCCTTGAGG GGACAGTCTC CACTAGTGCA ATCACAGAAA GCGAAACAGC AGTGGCCCAG 
ACTCTTACCT CTGTTGGAAC TTCGGTGACA GTTAGAAGGA ACCCCCTGGA AAACACTTCA 
ACACTCTCTA TTGAGACACA AAGCCACACT GAGGTCTTGG GCACCATCAC AGTCCCCATG 
GTTGCTGGAT CAACAATGGG AGAAGCAGCC TCCTTCGTGA GTTTCACGGC TCTCGACAGC 
AGCAGTCTCT CAGTGGTGGT CACCACCGAG AGCTCTGCTA CCTCAGAGAC TTTAACCACA 
GGCAATACAA CCAACAGCTC CTTCCTCACA GAGAGCCACC CTCCTTTTTC TATCTATTCA 
ACTACAGCTA GCACCAGCAA AAACCCAAAC ATCACCTTAA CCAAGACCAC AGCCTCACCA 
AAGCCCCCGA CGCATCCTAC AACATCTGCA TCCACTGCTT GGATAAGGAA AACCACAAAA 
CATGATCCAG GTGAAGATGG AGGCTTCCTC CTTGTACGGC TGACCGTGGC CTCCCCTAAG 
GACCTCACTG AGCACAACGC CAGAGAGAAG CTGATGAACC AGCTCCGCCG TGAACTGCAC 
GCCCGCATGC CACTTGTCCA CATGTCCTTC CTGAGCATCA GGAGGGGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Interleukin-2-mTORc1 Kinase Axis Defines the Signaling, Differentiation, and Metabolism of T Helper 1 and Follicular B Helper T Cells.
Immunity43(4)690-702(2015 Oct)
Ray JP,Staron MM,Shyer JA,Ho PC,Marshall HD,Gray SM,Laidlaw BJ,Araki K,Ahmed R,Kaech SM,Craft J

A mouse knockout library for secreted and transmembrane proteins.
Nature biotechnology28(7)749-55(2010 Jul)
Tang T,Li L,Tang J,Li Y,Lin WY,Martin F,Grant D,Solloway M,Parker L,Ye W,Forrest W,Ghilardi N,Oravecz T,Platt KA,Rice DS,Hansen GM,Abuin A,Eberhart DE,Godowski P,Holt KH,Peterson A,Zambrowicz BP,de Sauvage FJ

MUC20 suppresses the hepatocyte growth factor-induced Grb2-Ras pathway by binding to a multifunctional docking site of met.
Molecular and cellular biology24(17)7456-68(2004 Sep)
Higuchi T,Orita T,Katsuya K,Yamasaki Y,Akiyama K,Li H,Yamamoto T,Saito Y,Nakamura M

Molecular cloning, genomic structure, and expression analysis of MUC20, a novel mucin protein, up-regulated in injured kidney.
The Journal of biological chemistry279(3)1968-79(2004 Jan)
Higuchi T,Orita T,Nakanishi S,Katsuya K,Watanabe H,Yamasaki Y,Waga I,Nanayama T,Yamamoto Y,Munger W,Sun HW,Falk RJ,Jennette JC,Alcorta DA,Li H,Yamamoto T,Saito Y,Nakamura M

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Muc20 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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