Ccn5 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ccn5
Entrez Gene ID	22403
Full Name	WNT1 inducible signaling pathway protein 2
Synonyms	Ccn5,Crgr4,Ctgfl,Rcop1
General protein information	Preferred Names WNT1 inducible signaling pathway protein 2 Names WNT1-inducible-signaling pathway protein 2 CCN family member 5 CTGF-L WISP-2 connective tissue growth factor-like protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 H3

mRNA and Protein(s)

mRNA	Protein	Name
NM_016873.2	NP_058569.2	WNT1-inducible-signaling pathway protein 2 precursor
XM_006499169.3	XP_006499232.1	WNT1-inducible-signaling pathway protein 2 isoform X1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	WISP2	XP_004947088.1
Pan troglodytes (chimpanzee)	WISP2	XP_514666.1
	LOC717872	XP_001109859.1
Bos taurus (cattle)	WISP2	NP_001095646.1
Homo sapiens (human)	WISP2	NP_003872.1
Canis lupus familiaris (dog)	WISP2	XP_005635161.1
Mus musculus (house mouse)	Wisp2	NP_058569.2
Rattus norvegicus (Norway rat)	Wisp2	NP_113778.1

Gene Ontology
Bibliography

Related articles in PubMed

CCN5/WISP-2 restores ER-? in normal and neoplastic breast cells and sensitizes triple negative breast cancer cells to tamoxifen.
Sarkar S, Ghosh A, Banerjee S, Maity G, Das A, Larson MA, Gupta V, Haque I, Tawfik O, Banerjee SK
Oncogenesis6(5)e340(2017 May)

Overexpressing the novel autocrine/endocrine adipokine WISP2 induces hyperplasia of the heart, white and brown adipose tissues and prevents insulin resistance.
Gr?nberg JR, Hoffmann JM, Hedjazifar S, Nerstedt A, Jenndahl L, Elvin J, Castellot J, Wei L, Mov?rare-Skrtic S, Ohlsson C, Holm LM, B?ckhed F, Syed I, Bosch F, Saghatelian A, Kahn BB, Hammarstedt A, Smith U
Scientific reports743515(2017 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Insulin-like growth factor (IGF)-1 elicited clear stimulation of WNT1 inducible signaling pathway protein 2 (CCN5/WISP2) mRNA and protein levels in cultured islets.
Title: Role of CCN5 (WNT1 inducible signaling pathway protein 2) in pancreatic islets.

Data suggest that CCN5, minimally expressed in unstimulated islet beta-cells, is strongly and directly induced by Igf1 (insulin-like growth factor I); overexpression of CCN5 stimulates cell proliferation and inhibits streptozotocin-induced apoptosis.
Title: IGF-I stimulates CCN5/WISP2 gene expression in pancreatic ?-cells, which promotes cell proliferation and survival against streptozotocin.

These results suggested that secreted Wisp2/CCN5 induces Akt and ERK phosphorylation via integrins, and consequently facilitates neurite formation and conferred resistance to apoptosis.
Title: Wisp2/CCN5 up-regulated in the central nervous system of GM3-only mice facilitates neurite formation in Neuro2a cells via integrin-Akt signaling.

CCN2 is pro-hypertrophic and -fibrotic, whereas CCN5 is anti-hypertrophic and -fibrotic.
Title: The opposing effects of CCN2 and CCN5 on the development of cardiac hypertrophy and fibrosis.

Although WISP-2 is a downstream protein of Wnt signaling, the role of WISP-2 on adipocyte differentiation may be marginal, at least in this in vitro culture model.
Title: Effect of Wnt-1 inducible signaling pathway protein-2 (WISP-2/CCN5), a downstream protein of Wnt signaling, on adipocyte differentiation.

The following Ccn5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ccn5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu10611	NM_016873.2 Latest version!	Mus musculus cellular communication network factor 5 (Ccn5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu30251	XM_006499169.3 Latest version!	Mus musculus cellular communication network factor 5 (Ccn5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ccn5 ( NM_016873.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_058569.2 Mus musculus cellular communication network factor 5 (Ccn5), mRNA.

Price & Availability↑

CloneID	OMu10611
Clone ID Related Accession (Same CDS sequence)	NM_016873.2
Accession Version	NM_016873.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 756bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	WNT1-inducible-signaling pathway protein 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BQ560868.1, AL731698.10 and BC032877.1. On Aug 8, 2009 this sequence version replaced NM_016873.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF100778.1, AF126063.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGGGGCA ACCCACTGAT CCATCTTCTG GCCATTTCCT TCCTCTGCAT TCTCTCAATG 
GTGTATGCCC AGCTGTGCCC AGCACCCTGT GCCTGTCCTT GGACACCACC CCAGTGCCCA 
CCGGGGGTAC CCCTGGTGCT GGATGGCTGT GGCTGCTGTC GAGTGTGTGC ACGGAGGCTG 
GGGGAGTCCT GCGACCACCT GCATGTCTGC GACCCCAGCC AGGGCCTGGT TTGTCAGCCT 
GGGGCAGGCC CCAGTGGCCG TGGTGCTGTG TGCCTCTTCG AAGAGGATGA CGGGAGCTGT 
GAGGTGAATG GCCGCAGGTA CCTGGATGGG GAGACCTTTA AACCCAATTG CAGGGTTTTG 
TGCCGCTGTG ATGACGGTGG TTTCACCTGC CTGCCGCTGT GCAGTGAGGA TGTGCGGCTG 
CCCAGCTGGG ACTGCCCACG CCCCAGGAGA ATACAGGTGC CAGGAAGGTG CTGCCCCGAG 
TGGGTGTGTG ACCAGGCAGT GATGCAGCCG GCAATCCAGC CCTCCTCAGC CCAAGGACAC 
CAACTTTCTG CCCTTGTCAC TCCTGCATCT GCCGATGGCC CCTGTCCAAA CTGGAGCACA 
GCCTGGGGCC CCTGCTCAAC CACCTGTGGG TTGGGCATAG CCACCCGAGT ATCCAACCAG 
AACCGATTCT GCCAACTGGA GATCCAGCGT CGCCTGTGTC TGTCCAGACC CTGCCTGGCA 
TCCAGGAGCC ACGGCTCATG GAACAGTGCC TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058569.2
CDS	310..1065
Translation	MRGNPLIHLLAISFLCILSMVYAQLCPAPCACPWTPPQCPPGVPLVLDGCGCCRVCARRLGESCDHLHVCDPSQGLVCQPGAGPSGRGAVCLFEEDDGSCEVNGRRYLDGETFKPNCRVLCRCDDGGFTCLPLCSEDVRLPSWDCPRPRRIQVPGRCCPEWVCDQAVMQPAIQPSSAQGHQLSALVTPASADGPCPNWSTAWGPCSTTCGLGIATRVSNQNRFCQLEIQRRLCLSRPCLASRSHGSWNSAF

Target ORF information:

RefSeq Version	NM_016873.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus cellular communication network factor 5 (Ccn5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016873.2

ORF Insert Sequence:

ATGAGGGGCA ACCCACTGAT CCATCTTCTG GCCATTTCCT TCCTCTGCAT TCTCTCAATG 
GTGTATGCCC AGCTGTGCCC AGCACCCTGT GCCTGTCCTT GGACACCACC CCAGTGCCCA 
CCGGGGGTAC CCCTGGTGCT GGATGGCTGT GGCTGCTGTC GAGTGTGTGC ACGGAGGCTG 
GGGGAGTCCT GCGACCACCT GCATGTCTGC GACCCCAGCC AGGGCCTGGT TTGTCAGCCT 
GGGGCAGGCC CCAGTGGCCG TGGTGCTGTG TGCCTCTTCG AAGAGGATGA CGGGAGCTGT 
GAGGTGAATG GCCGCAGGTA CCTGGATGGG GAGACCTTTA AACCCAATTG CAGGGTTTTG 
TGCCGCTGTG ATGACGGTGG TTTCACCTGC CTGCCGCTGT GCAGTGAGGA TGTGCGGCTG 
CCCAGCTGGG ACTGCCCACG CCCCAGGAGA ATACAGGTGC CAGGAAGGTG CTGCCCCGAG 
TGGGTGTGTG ACCAGGCAGT GATGCAGCCG GCAATCCAGC CCTCCTCAGC CCAAGGACAC 
CAACTTTCTG CCCTTGTCAC TCCTGCATCT GCCGATGGCC CCTGTCCAAA CTGGAGCACA 
GCCTGGGGCC CCTGCTCAAC CACCTGTGGG TTGGGCATAG CCACCCGAGT ATCCAACCAG 
AACCGATTCT GCCAACTGGA GATCCAGCGT CGCCTGTGTC TGTCCAGACC CTGCCTGGCA 
TCCAGGAGCC ACGGCTCATG GAACAGTGCC TTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ccn5 ( XM_006499169.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006499232.1 Mus musculus cellular communication network factor 5 (Ccn5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu30251
Clone ID Related Accession (Same CDS sequence)	XM_006499169.3
Accession Version	XM_006499169.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 570bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	WNT1-inducible-signaling pathway protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006499169.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGGCA ACCCACTGAT CCATCTTCTG GCCATTTCCT TCCTCTGCAT TCTCTCAATG 
GTGTATGCCC AGCTGTGCCC AGCACCCTGT GCCTGTCCTT GGACACCACC CCAGTGCCCA 
CCGGGGGTAC CCCTGGTGCT GGATGGCTGT GGCTGCTGTC GAGTGTGTGC ACGGAGGCTG 
GGGGAGTCCT GCGACCACCT GCATGTCTGC GACCCCAGCC AGGGCCTGGT TTGTCAGCCT 
GGGGCAGGCC CCAGTGGCCG TGGTGCTGTG TGCCTCTTCG AAGAGGATGA CGGGAGCTGT 
GAGGTGAATG GCCGCAGGTA CCTGGATGGG GAGACCTTTA AACCCAATTG CAGGGTTTTG 
TGCCGCTGTG ATGACGGTGG TTTCACCTGC CTGCCGCTGT GCAGTGAGGA TGTGCGGCTG 
CCCAGCTGGG ACTGCCCACG CCCCAGGAGA ATACAGGTGC CAGGAAGGTG CTGCCCCGAG 
TGGGTGTGTG ACCAGGCAGT GATGCAGCCG GCAATCCAGC CCTCCTCAGC CCAAGGGTTG 
GAGATCCATC ACAACGTCGG CCTTTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006499232.1
CDS	392..961
Translation	MRGNPLIHLLAISFLCILSMVYAQLCPAPCACPWTPPQCPPGVPLVLDGCGCCRVCARRLGESCDHLHVCDPSQGLVCQPGAGPSGRGAVCLFEEDDGSCEVNGRRYLDGETFKPNCRVLCRCDDGGFTCLPLCSEDVRLPSWDCPRPRRIQVPGRCCPEWVCDQAVMQPAIQPSSAQGLEIHHNVGLC

Target ORF information:

RefSeq Version	XM_006499169.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus cellular communication network factor 5 (Ccn5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499169.3

ORF Insert Sequence:

ATGAGGGGCA ACCCACTGAT CCATCTTCTG GCCATTTCCT TCCTCTGCAT TCTCTCAATG 
GTGTATGCCC AGCTGTGCCC AGCACCCTGT GCCTGTCCTT GGACACCACC CCAGTGCCCA 
CCGGGGGTAC CCCTGGTGCT GGATGGCTGT GGCTGCTGTC GAGTGTGTGC ACGGAGGCTG 
GGGGAGTCCT GCGACCACCT GCATGTCTGC GACCCCAGCC AGGGCCTGGT TTGTCAGCCT 
GGGGCAGGCC CCAGTGGCCG TGGTGCTGTG TGCCTCTTCG AAGAGGATGA CGGGAGCTGT 
GAGGTGAATG GCCGCAGGTA CCTGGATGGG GAGACCTTTA AACCCAATTG CAGGGTTTTG 
TGCCGCTGTG ATGACGGTGG TTTCACCTGC CTGCCGCTGT GCAGTGAGGA TGTGCGGCTG 
CCCAGCTGGG ACTGCCCACG CCCCAGGAGA ATACAGGTGC CAGGAAGGTG CTGCCCCGAG 
TGGGTGTGTG ACCAGGCAGT GATGCAGCCG GCAATCCAGC CCTCCTCAGC CCAAGGGTTG 
GAGATCCATC ACAACGTCGG CCTTTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CCN5/WISP-2 restores ER-? in normal and neoplastic breast cells and sensitizes triple negative breast cancer cells to tamoxifen.
Oncogenesis6(5)e340(2017 May)
Sarkar S,Ghosh A,Banerjee S,Maity G,Das A,Larson MA,Gupta V,Haque I,Tawfik O,Banerjee SK

Overexpressing the novel autocrine/endocrine adipokine WISP2 induces hyperplasia of the heart, white and brown adipose tissues and prevents insulin resistance.
Scientific reports743515(2017 Feb)
Gr?nberg JR,Hoffmann JM,Hedjazifar S,Nerstedt A,Jenndahl L,Elvin J,Castellot J,Wei L,Mov?rare-Skrtic S,Ohlsson C,Holm LM,B?ckhed F,Syed I,Bosch F,Saghatelian A,Kahn BB,Hammarstedt A,Smith U

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Ccn5 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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