Wars cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Wars
Entrez Gene ID	22375
Full Name	tryptophanyl-tRNA synthetase
Synonyms	TrpRS,WRS
General protein information	Preferred Names tryptophanyl-tRNA synthetase Names tryptophan--tRNA ligase, cytoplasmic tryptophanyl-tRNA synthetase, cytoplasmic
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12\|12 F1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001164314.1	NP_001157786.1	tryptophan--tRNA ligase, cytoplasmic isoform 2
XM_006515819.1	XP_006515882.1	tryptophan--tRNA ligase, cytoplasmic isoform X1
NM_001164488.1	NP_001157960.1	tryptophan--tRNA ligase, cytoplasmic isoform 2
NM_011710.3	NP_035840.3	tryptophan--tRNA ligase, cytoplasmic isoform 1
XM_006515819.2	XP_006515882.1	tryptophan--tRNA ligase, cytoplasmic isoform X1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	WARS	XP_004936372.1
Danio rerio (zebrafish)	wars	NP_957066.1
Caenorhabditis elegans (roundworm)	wars-1	NP_507683.2
Magnaporthe oryzae (rice blast fungus)	MGG_09540	XP_003712201.1
Arabidopsis thaliana (thale cress)	AT3G04600	NP_974219.1
Kluyveromyces lactis	KLLA0B07733g	XP_451874.1
Neurospora crassa	NCU06722	XP_960902.1
Pan troglodytes (chimpanzee)	WARS	XP_003952625.1
Bos taurus (cattle)	WARS	NP_776643.1
Drosophila melanogaster (fruit fly)	Aats-trp	NP_524826.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003315	XP_319546.5
Schizosaccharomyces pombe (fission yeast)	wrs1	NP_592983.1
Oryza sativa (rice)	Os12g0540900	NP_001066951.1
Homo sapiens (human)	WARS	NP_776049.1
	WARS	XP_001105786.1
Canis lupus familiaris (dog)	WARS	XP_537554.1
Mus musculus (house mouse)	Wars	NP_035840.3
Saccharomyces cerevisiae (baker's yeast)	WRS1	NP_014544.1
Xenopus tropicalis (tropical clawed frog)	wars	NP_001039220.1
Rattus norvegicus (Norway rat)	Wars	NP_001013188.2
Eremothecium gossypii	AGOS_ADR117W	NP_984213.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

Species-specific differences in the regulation of the aminoacylation activity of mammalian tryptophanyl-tRNA synthetases.
Wakasugi K
FEBS letters584(1)229-32(2010 Jan)

Tryptamine induces tryptophanyl-tRNA synthetase-mediated neurodegeneration with neurofibrillary tangles in human cell and mouse models.
Paley EL, Denisova G, Sokolova O, Posternak N, Wang X, Brownell AL
Neuromolecular medicine9(1)55-82(2007)

Aberrant regulation of imprinted gene expression in Gtl2lacZ mice.
Sekita Y, Wagatsuma H, Irie M, Kobayashi S, Kohda T, Matsuda J, Yokoyama M, Ogura A, Schuster-Gossler K, Gossler A, Ishino F, Kaneko-Ishino T
Cytogenetic and genome research113(1-4)223-9(2006)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

TrpRS plays a role in protecting against neurodegeneration is suggested, providing an insight into the pathogenesis and a possible treatment of neurodegenerative diseases.
Title: Tryptamine induces tryptophanyl-tRNA synthetase-mediated neurodegeneration with neurofibrillary tangles in human cell and mouse models.

The following Wars gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Wars cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu20337	NM_011710.3 Latest version!	Mus musculus tryptophanyl-tRNA synthetase (Wars), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu20492	NM_001164314.1 Latest version!	Mus musculus tryptophanyl-tRNA synthetase (Wars), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu20492	NM_001164488.1 Latest version!	Mus musculus tryptophanyl-tRNA synthetase (Wars), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu20337	XM_006515819.2 Latest version!	Mus musculus tryptophanyl-tRNA synthetase (Wars), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OMu20337	XM_006515819.1	Mus musculus tryptophanyl-tRNA synthetase (Wars), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Wars ( NM_011710.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035840.3 Mus musculus tryptophanyl-tRNA synthetase (Wars), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu20337
Clone ID Related Accession (Same CDS sequence)	XM_006515819.1 , NM_011710.3 , XM_006515819.2
Accession Version	NM_011710.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1446bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Mus musculus(house mouse)
Product	tryptophan--tRNA ligase, cytoplasmic isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC122811.4, CD563585.1, CF731228.1 and AC152061.6. On Aug 29, 2009 this sequence version replaced NM_011710.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC003450.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381 [ECO:0000348] ##Evidence-Data-END##

ATGGCAGACA TGCCCAGTGG CGAGTCCTGC ACCTCTCCAC TGGAGCTGTT CAACAGCATA 
GCTACTCAAG GGGAGCTCGT GAGGTCCCTC AAAGCTGGAA ATGCACCAAA AGATGAGATT 
GATTCTGCAG TAAAGATGTT GTTATCATTA AAAATGAGCT ATAAGGCCGC CATGGGGGAG 
GAGTACAAGG CTGGCTGCCC TCCTGGGAAC CCAACAGCTG GGAGAAATTG TGACTCTGAT 
GCCACAAAAG CCAGCGAGGA TTTTGTGGAT CCATGGACGG TGCGGACAAG CAGTGCAAAA 
GGCATCGACT ATGACAAGCT CATTGTTCAG TTTGGAAGCA GCAAAATTGA CAAAGAGCTG 
ATAAACCGAA TAGAGCGAGC CACTGGCCAA AGACCGCACC GCTTCCTGCG CCGGGGCATC 
TTCTTCTCCC ACCGAGATAT GAATCAAATT CTGGATGCCT ATGAAAATAA GAAGCCATTC 
TACCTGTACA CAGGCAGGGG CCCCTCCTCC GAAGCAATGC ATCTGGGTCA TCTCGTCCCG 
TTTATTTTCA CAAAGTGGCT ACAGGATGTG TTCAATGTGC CTTTGGTCAT CCAGATGTCT 
GACGACGAGA AGTACCTGTG GAAGGACTTG ACTCTGGAGC AGGCATATAG CTACACCGTA 
GAAAATGCAA AGGACATCAT TGCCTGTGGC TTCGACATCA ACAAAACTTT CATCTTCTCT 
GATCTTGAGT ATATGGGGCA GAGCCCGGGC TTCTACAGGA ACGTGGTGAA GATTCAGAAG 
CACGTCACCT TCAACCAAGT GAAAGGCATT TTCGGCTTTA CGGACAGTGA CTGCATCGGG 
AAGATCAGTT TTCCTGCTGT CCAGGCCGCG CCATCGTTCA GCAACTCTTT CCCGAAGATC 
TTCCGAGACA GGACAGATAT CCAGTGCCTC ATCCCGTGTG CCATTGACCA GGATCCGTAC 
TTCAGAATGA CAAGGGACGT GGCCCCCAGG ATCGGCCATC CTAAACCTGC CCTGCTGCAC 
TCGACCTTCT TCCCTGCCCT GCAGGGCGCT CAGACCAAGA TGAGTGCCAG TGACCCCAAC 
TCCTCCATCT TCCTCACTGA CACGGCCAAG CAGATCAAGA GCAAGGTCAA CAAGCATGCA 
TTTTCTGGAG GAAGAGATAC TGTGGAGGAG CACAGGCAGT TTGGGGGCAA CTGTGAAGTG 
GATGTATCCT TCATGTATCT GACCTTCTTC CTTGAAGATG ACGACAGGCT GGAGCAGATC 
AGAAAGGATT ACACCAGCGG AGCCATGCTC ACCGGGGAGC TCAAGAAGAC GCTCATAGAC 
GTCCTTCAGC CCCTGATCGC AGAGCACCAG GCCCGACGCA AGGCGGTCAC TGAGGAGACC 
GTGAAGGAGT TCATGACGCC CCGGCAGCTG TCCTTCCACT TCCAGTGCTT CTGCTTTGAC 
ACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035840.3
CDS	115..1560
Translation	MADMPSGESCTSPLELFNSIATQGELVRSLKAGNAPKDEIDSAVKMLLSLKMSYKAAMGEEYKAGCPPGNPTAGRNCDSDATKASEDFVDPWTVRTSSAKGIDYDKLIVQFGSSKIDKELINRIERATGQRPHRFLRRGIFFSHRDMNQILDAYENKKPFYLYTGRGPSSEAMHLGHLVPFIFTKWLQDVFNVPLVIQMSDDEKYLWKDLTLEQAYSYTVENAKDIIACGFDINKTFIFSDLEYMGQSPGFYRNVVKIQKHVTFNQVKGIFGFTDSDCIGKISFPAVQAAPSFSNSFPKIFRDRTDIQCLIPCAIDQDPYFRMTRDVAPRIGHPKPALLHSTFFPALQGAQTKMSASDPNSSIFLTDTAKQIKSKVNKHAFSGGRDTVEEHRQFGGNCEVDVSFMYLTFFLEDDDRLEQIRKDYTSGAMLTGELKKTLIDVLQPLIAEHQARRKAVTEETVKEFMTPRQLSFHFQCFCFDT

Target ORF information:

RefSeq Version	NM_011710.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus tryptophanyl-tRNA synthetase (Wars), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011710.3

ORF Insert Sequence:

ATGGCAGACA TGCCCAGTGG CGAGTCCTGC ACCTCTCCAC TGGAGCTGTT CAACAGCATA 
GCTACTCAAG GGGAGCTCGT GAGGTCCCTC AAAGCTGGAA ATGCACCAAA AGATGAGATT 
GATTCTGCAG TAAAGATGTT GTTATCATTA AAAATGAGCT ATAAGGCCGC CATGGGGGAG 
GAGTACAAGG CTGGCTGCCC TCCTGGGAAC CCAACAGCTG GGAGAAATTG TGACTCTGAT 
GCCACAAAAG CCAGCGAGGA TTTTGTGGAT CCATGGACGG TGCGGACAAG CAGTGCAAAA 
GGCATCGACT ATGACAAGCT CATTGTTCAG TTTGGAAGCA GCAAAATTGA CAAAGAGCTG 
ATAAACCGAA TAGAGCGAGC CACTGGCCAA AGACCGCACC GCTTCCTGCG CCGGGGCATC 
TTCTTCTCCC ACCGAGATAT GAATCAAATT CTGGATGCCT ATGAAAATAA GAAGCCATTC 
TACCTGTACA CAGGCAGGGG CCCCTCCTCC GAAGCAATGC ATCTGGGTCA TCTCGTCCCG 
TTTATTTTCA CAAAGTGGCT ACAGGATGTG TTCAATGTGC CTTTGGTCAT CCAGATGTCT 
GACGACGAGA AGTACCTGTG GAAGGACTTG ACTCTGGAGC AGGCATATAG CTACACCGTA 
GAAAATGCAA AGGACATCAT TGCCTGTGGC TTCGACATCA ACAAAACTTT CATCTTCTCT 
GATCTTGAGT ATATGGGGCA GAGCCCGGGC TTCTACAGGA ACGTGGTGAA GATTCAGAAG 
CACGTCACCT TCAACCAAGT GAAAGGCATT TTCGGCTTTA CGGACAGTGA CTGCATCGGG 
AAGATCAGTT TTCCTGCTGT CCAGGCCGCG CCATCGTTCA GCAACTCTTT CCCGAAGATC 
TTCCGAGACA GGACAGATAT CCAGTGCCTC ATCCCGTGTG CCATTGACCA GGATCCGTAC 
TTCAGAATGA CAAGGGACGT GGCCCCCAGG ATCGGCCATC CTAAACCTGC CCTGCTGCAC 
TCGACCTTCT TCCCTGCCCT GCAGGGCGCT CAGACCAAGA TGAGTGCCAG TGACCCCAAC 
TCCTCCATCT TCCTCACTGA CACGGCCAAG CAGATCAAGA GCAAGGTCAA CAAGCATGCA 
TTTTCTGGAG GAAGAGATAC TGTGGAGGAG CACAGGCAGT TTGGGGGCAA CTGTGAAGTG 
GATGTATCCT TCATGTATCT GACCTTCTTC CTTGAAGATG ACGACAGGCT GGAGCAGATC 
AGAAAGGATT ACACCAGCGG AGCCATGCTC ACCGGGGAGC TCAAGAAGAC GCTCATAGAC 
GTCCTTCAGC CCCTGATCGC AGAGCACCAG GCCCGACGCA AGGCGGTCAC TGAGGAGACC 
GTGAAGGAGT TCATGACGCC CCGGCAGCTG TCCTTCCACT TCCAGTGCTT CTGCTTTGAC 
ACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Wars ( NM_001164314.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001157786.1 Mus musculus tryptophanyl-tRNA synthetase (Wars), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu20492
Clone ID Related Accession (Same CDS sequence)	NM_001164314.1 , NM_001164488.1
Accession Version	NM_001164314.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1428bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-14
Organism	Mus musculus(house mouse)
Product	tryptophan--tRNA ligase, cytoplasmic isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY773669.1, AK152966.1, AC122811.4 and AC152061.6. Transcript Variant: This variant (2) differs in the 5' UTR, 3' UTR, and coding region, compared to variant 1. The encoded isoform (2) has a shorter C-terminus compared to isoform 1. Variants 2 and 3 encode the same isoform (2). ##Evidence-Data-START## Transcript exon combination :: AK153337.1, AK150090.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164143 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGACA TGCCCAGTGG CGAGTCCTGC ACCTCTCCAC TGGAGCTGTT CAACAGCATA 
GCTACTCAAG GGGAGCTCGT GAGGTCCCTC AAAGCTGGAA ATGCACCAAA AGATGAGATT 
GATTCTGCAG TAAAGATGTT GTTATCATTA AAAATGAGCT ATAAGGCCGC CATGGGGGAG 
GAGTACAAGG CTGGCTGCCC TCCTGGGAAC CCAACAGCTG GGAGAAATTG TGACTCTGAT 
GCCACAAAAG CCAGCGAGGA TTTTGTGGAT CCATGGACGG TGCGGACAAG CAGTGCAAAA 
GGCATCGACT ATGACAAGCT CATTGTTCAG TTTGGAAGCA GCAAAATTGA CAAAGAGCTG 
ATAAACCGAA TAGAGCGAGC CACTGGCCAA AGACCGCACC GCTTCCTGCG CCGGGGCATC 
TTCTTCTCCC ACCGAGATAT GAATCAAATT CTGGATGCCT ATGAAAATAA GAAGCCATTC 
TACCTGTACA CAGGCAGGGG CCCCTCCTCC GAAGCAATGC ATCTGGGTCA TCTCGTCCCG 
TTTATTTTCA CAAAGTGGCT ACAGGATGTG TTCAATGTGC CTTTGGTCAT CCAGATGTCT 
GACGACGAGA AGTACCTGTG GAAGGACTTG ACTCTGGAGC AGGCATATAG CTACACCGTA 
GAAAATGCAA AGGACATCAT TGCCTGTGGC TTCGACATCA ACAAAACTTT CATCTTCTCT 
GATCTTGAGT ATATGGGGCA GAGCCCGGGC TTCTACAGGA ACGTGGTGAA GATTCAGAAG 
CACGTCACCT TCAACCAAGT GAAAGGCATT TTCGGCTTTA CGGACAGTGA CTGCATCGGG 
AAGATCAGTT TTCCTGCTGT CCAGGCCGCG CCATCGTTCA GCAACTCTTT CCCGAAGATC 
TTCCGAGACA GGACAGATAT CCAGTGCCTC ATCCCGTGTG CCATTGACCA GGATCCGTAC 
TTCAGAATGA CAAGGGACGT GGCCCCCAGG ATCGGCCATC CTAAACCTGC CCTGCTGCAC 
TCGACCTTCT TCCCTGCCCT GCAGGGCGCT CAGACCAAGA TGAGTGCCAG TGACCCCAAC 
TCCTCCATCT TCCTCACTGA CACGGCCAAG CAGATCAAGA GCAAGGTCAA CAAGCATGCA 
TTTTCTGGAG GAAGAGATAC TGTGGAGGAG CACAGGCAGT TTGGGGGCAA CTGTGAAGTG 
GATGTATCCT TCATGTATCT GACCTTCTTC CTTGAAGATG ACGACAGGCT GGAGCAGATC 
AGAAAGGATT ACACCAGCGG AGCCATGCTC ACCGGGGAGC TCAAGAAGAC GCTCATAGAC 
GTCCTTCAGC CCCTGATCGC AGAGCACCAG GCCCGACGCA AGGCGGTCAC TGAGGAGACC 
GTGAAGGAGT TCATGACGCC CCGGCAGCTG TCCTTCCACT TCCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001157786.1
CDS	169..1596
Translation	MADMPSGESCTSPLELFNSIATQGELVRSLKAGNAPKDEIDSAVKMLLSLKMSYKAAMGEEYKAGCPPGNPTAGRNCDSDATKASEDFVDPWTVRTSSAKGIDYDKLIVQFGSSKIDKELINRIERATGQRPHRFLRRGIFFSHRDMNQILDAYENKKPFYLYTGRGPSSEAMHLGHLVPFIFTKWLQDVFNVPLVIQMSDDEKYLWKDLTLEQAYSYTVENAKDIIACGFDINKTFIFSDLEYMGQSPGFYRNVVKIQKHVTFNQVKGIFGFTDSDCIGKISFPAVQAAPSFSNSFPKIFRDRTDIQCLIPCAIDQDPYFRMTRDVAPRIGHPKPALLHSTFFPALQGAQTKMSASDPNSSIFLTDTAKQIKSKVNKHAFSGGRDTVEEHRQFGGNCEVDVSFMYLTFFLEDDDRLEQIRKDYTSGAMLTGELKKTLIDVLQPLIAEHQARRKAVTEETVKEFMTPRQLSFHFQ

Target ORF information:

RefSeq Version	NM_001164314.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tryptophanyl-tRNA synthetase (Wars), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001164314.1

ORF Insert Sequence:

ATGGCAGACA TGCCCAGTGG CGAGTCCTGC ACCTCTCCAC TGGAGCTGTT CAACAGCATA 
GCTACTCAAG GGGAGCTCGT GAGGTCCCTC AAAGCTGGAA ATGCACCAAA AGATGAGATT 
GATTCTGCAG TAAAGATGTT GTTATCATTA AAAATGAGCT ATAAGGCCGC CATGGGGGAG 
GAGTACAAGG CTGGCTGCCC TCCTGGGAAC CCAACAGCTG GGAGAAATTG TGACTCTGAT 
GCCACAAAAG CCAGCGAGGA TTTTGTGGAT CCATGGACGG TGCGGACAAG CAGTGCAAAA 
GGCATCGACT ATGACAAGCT CATTGTTCAG TTTGGAAGCA GCAAAATTGA CAAAGAGCTG 
ATAAACCGAA TAGAGCGAGC CACTGGCCAA AGACCGCACC GCTTCCTGCG CCGGGGCATC 
TTCTTCTCCC ACCGAGATAT GAATCAAATT CTGGATGCCT ATGAAAATAA GAAGCCATTC 
TACCTGTACA CAGGCAGGGG CCCCTCCTCC GAAGCAATGC ATCTGGGTCA TCTCGTCCCG 
TTTATTTTCA CAAAGTGGCT ACAGGATGTG TTCAATGTGC CTTTGGTCAT CCAGATGTCT 
GACGACGAGA AGTACCTGTG GAAGGACTTG ACTCTGGAGC AGGCATATAG CTACACCGTA 
GAAAATGCAA AGGACATCAT TGCCTGTGGC TTCGACATCA ACAAAACTTT CATCTTCTCT 
GATCTTGAGT ATATGGGGCA GAGCCCGGGC TTCTACAGGA ACGTGGTGAA GATTCAGAAG 
CACGTCACCT TCAACCAAGT GAAAGGCATT TTCGGCTTTA CGGACAGTGA CTGCATCGGG 
AAGATCAGTT TTCCTGCTGT CCAGGCCGCG CCATCGTTCA GCAACTCTTT CCCGAAGATC 
TTCCGAGACA GGACAGATAT CCAGTGCCTC ATCCCGTGTG CCATTGACCA GGATCCGTAC 
TTCAGAATGA CAAGGGACGT GGCCCCCAGG ATCGGCCATC CTAAACCTGC CCTGCTGCAC 
TCGACCTTCT TCCCTGCCCT GCAGGGCGCT CAGACCAAGA TGAGTGCCAG TGACCCCAAC 
TCCTCCATCT TCCTCACTGA CACGGCCAAG CAGATCAAGA GCAAGGTCAA CAAGCATGCA 
TTTTCTGGAG GAAGAGATAC TGTGGAGGAG CACAGGCAGT TTGGGGGCAA CTGTGAAGTG 
GATGTATCCT TCATGTATCT GACCTTCTTC CTTGAAGATG ACGACAGGCT GGAGCAGATC 
AGAAAGGATT ACACCAGCGG AGCCATGCTC ACCGGGGAGC TCAAGAAGAC GCTCATAGAC 
GTCCTTCAGC CCCTGATCGC AGAGCACCAG GCCCGACGCA AGGCGGTCAC TGAGGAGACC 
GTGAAGGAGT TCATGACGCC CCGGCAGCTG TCCTTCCACT TCCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Wars ( NM_001164488.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001157960.1 Mus musculus tryptophanyl-tRNA synthetase (Wars), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu20492
Clone ID Related Accession (Same CDS sequence)	NM_001164314.1 , NM_001164488.1
Accession Version	NM_001164488.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1428bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Mus musculus(house mouse)
Product	tryptophan--tRNA ligase, cytoplasmic isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK004541.1 and AC152061.6. Transcript Variant: This variant (3) differs in the 3' UTR and 3' coding region, compared to variant 1. The encoded isoform (2) has a shorter C-terminus compared to isoform 1. Variants 2 and 3 encode the same isoform (2). ##Evidence-Data-START## Transcript exon combination :: AK004541.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGACA TGCCCAGTGG CGAGTCCTGC ACCTCTCCAC TGGAGCTGTT CAACAGCATA 
GCTACTCAAG GGGAGCTCGT GAGGTCCCTC AAAGCTGGAA ATGCACCAAA AGATGAGATT 
GATTCTGCAG TAAAGATGTT GTTATCATTA AAAATGAGCT ATAAGGCCGC CATGGGGGAG 
GAGTACAAGG CTGGCTGCCC TCCTGGGAAC CCAACAGCTG GGAGAAATTG TGACTCTGAT 
GCCACAAAAG CCAGCGAGGA TTTTGTGGAT CCATGGACGG TGCGGACAAG CAGTGCAAAA 
GGCATCGACT ATGACAAGCT CATTGTTCAG TTTGGAAGCA GCAAAATTGA CAAAGAGCTG 
ATAAACCGAA TAGAGCGAGC CACTGGCCAA AGACCGCACC GCTTCCTGCG CCGGGGCATC 
TTCTTCTCCC ACCGAGATAT GAATCAAATT CTGGATGCCT ATGAAAATAA GAAGCCATTC 
TACCTGTACA CAGGCAGGGG CCCCTCCTCC GAAGCAATGC ATCTGGGTCA TCTCGTCCCG 
TTTATTTTCA CAAAGTGGCT ACAGGATGTG TTCAATGTGC CTTTGGTCAT CCAGATGTCT 
GACGACGAGA AGTACCTGTG GAAGGACTTG ACTCTGGAGC AGGCATATAG CTACACCGTA 
GAAAATGCAA AGGACATCAT TGCCTGTGGC TTCGACATCA ACAAAACTTT CATCTTCTCT 
GATCTTGAGT ATATGGGGCA GAGCCCGGGC TTCTACAGGA ACGTGGTGAA GATTCAGAAG 
CACGTCACCT TCAACCAAGT GAAAGGCATT TTCGGCTTTA CGGACAGTGA CTGCATCGGG 
AAGATCAGTT TTCCTGCTGT CCAGGCCGCG CCATCGTTCA GCAACTCTTT CCCGAAGATC 
TTCCGAGACA GGACAGATAT CCAGTGCCTC ATCCCGTGTG CCATTGACCA GGATCCGTAC 
TTCAGAATGA CAAGGGACGT GGCCCCCAGG ATCGGCCATC CTAAACCTGC CCTGCTGCAC 
TCGACCTTCT TCCCTGCCCT GCAGGGCGCT CAGACCAAGA TGAGTGCCAG TGACCCCAAC 
TCCTCCATCT TCCTCACTGA CACGGCCAAG CAGATCAAGA GCAAGGTCAA CAAGCATGCA 
TTTTCTGGAG GAAGAGATAC TGTGGAGGAG CACAGGCAGT TTGGGGGCAA CTGTGAAGTG 
GATGTATCCT TCATGTATCT GACCTTCTTC CTTGAAGATG ACGACAGGCT GGAGCAGATC 
AGAAAGGATT ACACCAGCGG AGCCATGCTC ACCGGGGAGC TCAAGAAGAC GCTCATAGAC 
GTCCTTCAGC CCCTGATCGC AGAGCACCAG GCCCGACGCA AGGCGGTCAC TGAGGAGACC 
GTGAAGGAGT TCATGACGCC CCGGCAGCTG TCCTTCCACT TCCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001157960.1
CDS	102..1529
Translation	MADMPSGESCTSPLELFNSIATQGELVRSLKAGNAPKDEIDSAVKMLLSLKMSYKAAMGEEYKAGCPPGNPTAGRNCDSDATKASEDFVDPWTVRTSSAKGIDYDKLIVQFGSSKIDKELINRIERATGQRPHRFLRRGIFFSHRDMNQILDAYENKKPFYLYTGRGPSSEAMHLGHLVPFIFTKWLQDVFNVPLVIQMSDDEKYLWKDLTLEQAYSYTVENAKDIIACGFDINKTFIFSDLEYMGQSPGFYRNVVKIQKHVTFNQVKGIFGFTDSDCIGKISFPAVQAAPSFSNSFPKIFRDRTDIQCLIPCAIDQDPYFRMTRDVAPRIGHPKPALLHSTFFPALQGAQTKMSASDPNSSIFLTDTAKQIKSKVNKHAFSGGRDTVEEHRQFGGNCEVDVSFMYLTFFLEDDDRLEQIRKDYTSGAMLTGELKKTLIDVLQPLIAEHQARRKAVTEETVKEFMTPRQLSFHFQ

Target ORF information:

RefSeq Version	NM_001164488.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tryptophanyl-tRNA synthetase (Wars), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001164488.1

ORF Insert Sequence:

ATGGCAGACA TGCCCAGTGG CGAGTCCTGC ACCTCTCCAC TGGAGCTGTT CAACAGCATA 
GCTACTCAAG GGGAGCTCGT GAGGTCCCTC AAAGCTGGAA ATGCACCAAA AGATGAGATT 
GATTCTGCAG TAAAGATGTT GTTATCATTA AAAATGAGCT ATAAGGCCGC CATGGGGGAG 
GAGTACAAGG CTGGCTGCCC TCCTGGGAAC CCAACAGCTG GGAGAAATTG TGACTCTGAT 
GCCACAAAAG CCAGCGAGGA TTTTGTGGAT CCATGGACGG TGCGGACAAG CAGTGCAAAA 
GGCATCGACT ATGACAAGCT CATTGTTCAG TTTGGAAGCA GCAAAATTGA CAAAGAGCTG 
ATAAACCGAA TAGAGCGAGC CACTGGCCAA AGACCGCACC GCTTCCTGCG CCGGGGCATC 
TTCTTCTCCC ACCGAGATAT GAATCAAATT CTGGATGCCT ATGAAAATAA GAAGCCATTC 
TACCTGTACA CAGGCAGGGG CCCCTCCTCC GAAGCAATGC ATCTGGGTCA TCTCGTCCCG 
TTTATTTTCA CAAAGTGGCT ACAGGATGTG TTCAATGTGC CTTTGGTCAT CCAGATGTCT 
GACGACGAGA AGTACCTGTG GAAGGACTTG ACTCTGGAGC AGGCATATAG CTACACCGTA 
GAAAATGCAA AGGACATCAT TGCCTGTGGC TTCGACATCA ACAAAACTTT CATCTTCTCT 
GATCTTGAGT ATATGGGGCA GAGCCCGGGC TTCTACAGGA ACGTGGTGAA GATTCAGAAG 
CACGTCACCT TCAACCAAGT GAAAGGCATT TTCGGCTTTA CGGACAGTGA CTGCATCGGG 
AAGATCAGTT TTCCTGCTGT CCAGGCCGCG CCATCGTTCA GCAACTCTTT CCCGAAGATC 
TTCCGAGACA GGACAGATAT CCAGTGCCTC ATCCCGTGTG CCATTGACCA GGATCCGTAC 
TTCAGAATGA CAAGGGACGT GGCCCCCAGG ATCGGCCATC CTAAACCTGC CCTGCTGCAC 
TCGACCTTCT TCCCTGCCCT GCAGGGCGCT CAGACCAAGA TGAGTGCCAG TGACCCCAAC 
TCCTCCATCT TCCTCACTGA CACGGCCAAG CAGATCAAGA GCAAGGTCAA CAAGCATGCA 
TTTTCTGGAG GAAGAGATAC TGTGGAGGAG CACAGGCAGT TTGGGGGCAA CTGTGAAGTG 
GATGTATCCT TCATGTATCT GACCTTCTTC CTTGAAGATG ACGACAGGCT GGAGCAGATC 
AGAAAGGATT ACACCAGCGG AGCCATGCTC ACCGGGGAGC TCAAGAAGAC GCTCATAGAC 
GTCCTTCAGC CCCTGATCGC AGAGCACCAG GCCCGACGCA AGGCGGTCAC TGAGGAGACC 
GTGAAGGAGT TCATGACGCC CCGGCAGCTG TCCTTCCACT TCCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Wars ( XM_006515819.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515882.1 Mus musculus tryptophanyl-tRNA synthetase (Wars), transcript variant X1, mRNA.

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CloneID	OMu20337
Clone ID Related Accession (Same CDS sequence)	XM_006515819.1 , NM_011710.3 , XM_006515819.2
Accession Version	XM_006515819.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1446bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	tryptophan--tRNA ligase, cytoplasmic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGACA TGCCCAGTGG CGAGTCCTGC ACCTCTCCAC TGGAGCTGTT CAACAGCATA 
GCTACTCAAG GGGAGCTCGT GAGGTCCCTC AAAGCTGGAA ATGCACCAAA AGATGAGATT 
GATTCTGCAG TAAAGATGTT GTTATCATTA AAAATGAGCT ATAAGGCCGC CATGGGGGAG 
GAGTACAAGG CTGGCTGCCC TCCTGGGAAC CCAACAGCTG GGAGAAATTG TGACTCTGAT 
GCCACAAAAG CCAGCGAGGA TTTTGTGGAT CCATGGACGG TGCGGACAAG CAGTGCAAAA 
GGCATCGACT ATGACAAGCT CATTGTTCAG TTTGGAAGCA GCAAAATTGA CAAAGAGCTG 
ATAAACCGAA TAGAGCGAGC CACTGGCCAA AGACCGCACC GCTTCCTGCG CCGGGGCATC 
TTCTTCTCCC ACCGAGATAT GAATCAAATT CTGGATGCCT ATGAAAATAA GAAGCCATTC 
TACCTGTACA CAGGCAGGGG CCCCTCCTCC GAAGCAATGC ATCTGGGTCA TCTCGTCCCG 
TTTATTTTCA CAAAGTGGCT ACAGGATGTG TTCAATGTGC CTTTGGTCAT CCAGATGTCT 
GACGACGAGA AGTACCTGTG GAAGGACTTG ACTCTGGAGC AGGCATATAG CTACACCGTA 
GAAAATGCAA AGGACATCAT TGCCTGTGGC TTCGACATCA ACAAAACTTT CATCTTCTCT 
GATCTTGAGT ATATGGGGCA GAGCCCGGGC TTCTACAGGA ACGTGGTGAA GATTCAGAAG 
CACGTCACCT TCAACCAAGT GAAAGGCATT TTCGGCTTTA CGGACAGTGA CTGCATCGGG 
AAGATCAGTT TTCCTGCTGT CCAGGCCGCG CCATCGTTCA GCAACTCTTT CCCGAAGATC 
TTCCGAGACA GGACAGATAT CCAGTGCCTC ATCCCGTGTG CCATTGACCA GGATCCGTAC 
TTCAGAATGA CAAGGGACGT GGCCCCCAGG ATCGGCCATC CTAAACCTGC CCTGCTGCAC 
TCGACCTTCT TCCCTGCCCT GCAGGGCGCT CAGACCAAGA TGAGTGCCAG TGACCCCAAC 
TCCTCCATCT TCCTCACTGA CACGGCCAAG CAGATCAAGA GCAAGGTCAA CAAGCATGCA 
TTTTCTGGAG GAAGAGATAC TGTGGAGGAG CACAGGCAGT TTGGGGGCAA CTGTGAAGTG 
GATGTATCCT TCATGTATCT GACCTTCTTC CTTGAAGATG ACGACAGGCT GGAGCAGATC 
AGAAAGGATT ACACCAGCGG AGCCATGCTC ACCGGGGAGC TCAAGAAGAC GCTCATAGAC 
GTCCTTCAGC CCCTGATCGC AGAGCACCAG GCCCGACGCA AGGCGGTCAC TGAGGAGACC 
GTGAAGGAGT TCATGACGCC CCGGCAGCTG TCCTTCCACT TCCAGTGCTT CTGCTTTGAC 
ACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515882.1
CDS	164..1609
Translation	MADMPSGESCTSPLELFNSIATQGELVRSLKAGNAPKDEIDSAVKMLLSLKMSYKAAMGEEYKAGCPPGNPTAGRNCDSDATKASEDFVDPWTVRTSSAKGIDYDKLIVQFGSSKIDKELINRIERATGQRPHRFLRRGIFFSHRDMNQILDAYENKKPFYLYTGRGPSSEAMHLGHLVPFIFTKWLQDVFNVPLVIQMSDDEKYLWKDLTLEQAYSYTVENAKDIIACGFDINKTFIFSDLEYMGQSPGFYRNVVKIQKHVTFNQVKGIFGFTDSDCIGKISFPAVQAAPSFSNSFPKIFRDRTDIQCLIPCAIDQDPYFRMTRDVAPRIGHPKPALLHSTFFPALQGAQTKMSASDPNSSIFLTDTAKQIKSKVNKHAFSGGRDTVEEHRQFGGNCEVDVSFMYLTFFLEDDDRLEQIRKDYTSGAMLTGELKKTLIDVLQPLIAEHQARRKAVTEETVKEFMTPRQLSFHFQCFCFDT

Target ORF information:

RefSeq Version	XM_006515819.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus tryptophanyl-tRNA synthetase (Wars), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515819.1

ORF Insert Sequence:

ATGGCAGACA TGCCCAGTGG CGAGTCCTGC ACCTCTCCAC TGGAGCTGTT CAACAGCATA 
GCTACTCAAG GGGAGCTCGT GAGGTCCCTC AAAGCTGGAA ATGCACCAAA AGATGAGATT 
GATTCTGCAG TAAAGATGTT GTTATCATTA AAAATGAGCT ATAAGGCCGC CATGGGGGAG 
GAGTACAAGG CTGGCTGCCC TCCTGGGAAC CCAACAGCTG GGAGAAATTG TGACTCTGAT 
GCCACAAAAG CCAGCGAGGA TTTTGTGGAT CCATGGACGG TGCGGACAAG CAGTGCAAAA 
GGCATCGACT ATGACAAGCT CATTGTTCAG TTTGGAAGCA GCAAAATTGA CAAAGAGCTG 
ATAAACCGAA TAGAGCGAGC CACTGGCCAA AGACCGCACC GCTTCCTGCG CCGGGGCATC 
TTCTTCTCCC ACCGAGATAT GAATCAAATT CTGGATGCCT ATGAAAATAA GAAGCCATTC 
TACCTGTACA CAGGCAGGGG CCCCTCCTCC GAAGCAATGC ATCTGGGTCA TCTCGTCCCG 
TTTATTTTCA CAAAGTGGCT ACAGGATGTG TTCAATGTGC CTTTGGTCAT CCAGATGTCT 
GACGACGAGA AGTACCTGTG GAAGGACTTG ACTCTGGAGC AGGCATATAG CTACACCGTA 
GAAAATGCAA AGGACATCAT TGCCTGTGGC TTCGACATCA ACAAAACTTT CATCTTCTCT 
GATCTTGAGT ATATGGGGCA GAGCCCGGGC TTCTACAGGA ACGTGGTGAA GATTCAGAAG 
CACGTCACCT TCAACCAAGT GAAAGGCATT TTCGGCTTTA CGGACAGTGA CTGCATCGGG 
AAGATCAGTT TTCCTGCTGT CCAGGCCGCG CCATCGTTCA GCAACTCTTT CCCGAAGATC 
TTCCGAGACA GGACAGATAT CCAGTGCCTC ATCCCGTGTG CCATTGACCA GGATCCGTAC 
TTCAGAATGA CAAGGGACGT GGCCCCCAGG ATCGGCCATC CTAAACCTGC CCTGCTGCAC 
TCGACCTTCT TCCCTGCCCT GCAGGGCGCT CAGACCAAGA TGAGTGCCAG TGACCCCAAC 
TCCTCCATCT TCCTCACTGA CACGGCCAAG CAGATCAAGA GCAAGGTCAA CAAGCATGCA 
TTTTCTGGAG GAAGAGATAC TGTGGAGGAG CACAGGCAGT TTGGGGGCAA CTGTGAAGTG 
GATGTATCCT TCATGTATCT GACCTTCTTC CTTGAAGATG ACGACAGGCT GGAGCAGATC 
AGAAAGGATT ACACCAGCGG AGCCATGCTC ACCGGGGAGC TCAAGAAGAC GCTCATAGAC 
GTCCTTCAGC CCCTGATCGC AGAGCACCAG GCCCGACGCA AGGCGGTCAC TGAGGAGACC 
GTGAAGGAGT TCATGACGCC CCGGCAGCTG TCCTTCCACT TCCAGTGCTT CTGCTTTGAC 
ACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Wars ( XM_006515819.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515882.1 Mus musculus tryptophanyl-tRNA synthetase (Wars), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu20337
Clone ID Related Accession (Same CDS sequence)	XM_006515819.1 , NM_011710.3 , XM_006515819.2
Accession Version	XM_006515819.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1446bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	tryptophan--tRNA ligase, cytoplasmic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006515819.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGACA TGCCCAGTGG CGAGTCCTGC ACCTCTCCAC TGGAGCTGTT CAACAGCATA 
GCTACTCAAG GGGAGCTCGT GAGGTCCCTC AAAGCTGGAA ATGCACCAAA AGATGAGATT 
GATTCTGCAG TAAAGATGTT GTTATCATTA AAAATGAGCT ATAAGGCCGC CATGGGGGAG 
GAGTACAAGG CTGGCTGCCC TCCTGGGAAC CCAACAGCTG GGAGAAATTG TGACTCTGAT 
GCCACAAAAG CCAGCGAGGA TTTTGTGGAT CCATGGACGG TGCGGACAAG CAGTGCAAAA 
GGCATCGACT ATGACAAGCT CATTGTTCAG TTTGGAAGCA GCAAAATTGA CAAAGAGCTG 
ATAAACCGAA TAGAGCGAGC CACTGGCCAA AGACCGCACC GCTTCCTGCG CCGGGGCATC 
TTCTTCTCCC ACCGAGATAT GAATCAAATT CTGGATGCCT ATGAAAATAA GAAGCCATTC 
TACCTGTACA CAGGCAGGGG CCCCTCCTCC GAAGCAATGC ATCTGGGTCA TCTCGTCCCG 
TTTATTTTCA CAAAGTGGCT ACAGGATGTG TTCAATGTGC CTTTGGTCAT CCAGATGTCT 
GACGACGAGA AGTACCTGTG GAAGGACTTG ACTCTGGAGC AGGCATATAG CTACACCGTA 
GAAAATGCAA AGGACATCAT TGCCTGTGGC TTCGACATCA ACAAAACTTT CATCTTCTCT 
GATCTTGAGT ATATGGGGCA GAGCCCGGGC TTCTACAGGA ACGTGGTGAA GATTCAGAAG 
CACGTCACCT TCAACCAAGT GAAAGGCATT TTCGGCTTTA CGGACAGTGA CTGCATCGGG 
AAGATCAGTT TTCCTGCTGT CCAGGCCGCG CCATCGTTCA GCAACTCTTT CCCGAAGATC 
TTCCGAGACA GGACAGATAT CCAGTGCCTC ATCCCGTGTG CCATTGACCA GGATCCGTAC 
TTCAGAATGA CAAGGGACGT GGCCCCCAGG ATCGGCCATC CTAAACCTGC CCTGCTGCAC 
TCGACCTTCT TCCCTGCCCT GCAGGGCGCT CAGACCAAGA TGAGTGCCAG TGACCCCAAC 
TCCTCCATCT TCCTCACTGA CACGGCCAAG CAGATCAAGA GCAAGGTCAA CAAGCATGCA 
TTTTCTGGAG GAAGAGATAC TGTGGAGGAG CACAGGCAGT TTGGGGGCAA CTGTGAAGTG 
GATGTATCCT TCATGTATCT GACCTTCTTC CTTGAAGATG ACGACAGGCT GGAGCAGATC 
AGAAAGGATT ACACCAGCGG AGCCATGCTC ACCGGGGAGC TCAAGAAGAC GCTCATAGAC 
GTCCTTCAGC CCCTGATCGC AGAGCACCAG GCCCGACGCA AGGCGGTCAC TGAGGAGACC 
GTGAAGGAGT TCATGACGCC CCGGCAGCTG TCCTTCCACT TCCAGTGCTT CTGCTTTGAC 
ACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515882.1
CDS	170..1615
Translation	MADMPSGESCTSPLELFNSIATQGELVRSLKAGNAPKDEIDSAVKMLLSLKMSYKAAMGEEYKAGCPPGNPTAGRNCDSDATKASEDFVDPWTVRTSSAKGIDYDKLIVQFGSSKIDKELINRIERATGQRPHRFLRRGIFFSHRDMNQILDAYENKKPFYLYTGRGPSSEAMHLGHLVPFIFTKWLQDVFNVPLVIQMSDDEKYLWKDLTLEQAYSYTVENAKDIIACGFDINKTFIFSDLEYMGQSPGFYRNVVKIQKHVTFNQVKGIFGFTDSDCIGKISFPAVQAAPSFSNSFPKIFRDRTDIQCLIPCAIDQDPYFRMTRDVAPRIGHPKPALLHSTFFPALQGAQTKMSASDPNSSIFLTDTAKQIKSKVNKHAFSGGRDTVEEHRQFGGNCEVDVSFMYLTFFLEDDDRLEQIRKDYTSGAMLTGELKKTLIDVLQPLIAEHQARRKAVTEETVKEFMTPRQLSFHFQCFCFDT

Target ORF information:

RefSeq Version	XM_006515819.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus tryptophanyl-tRNA synthetase (Wars), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515819.2

ORF Insert Sequence:

ATGGCAGACA TGCCCAGTGG CGAGTCCTGC ACCTCTCCAC TGGAGCTGTT CAACAGCATA 
GCTACTCAAG GGGAGCTCGT GAGGTCCCTC AAAGCTGGAA ATGCACCAAA AGATGAGATT 
GATTCTGCAG TAAAGATGTT GTTATCATTA AAAATGAGCT ATAAGGCCGC CATGGGGGAG 
GAGTACAAGG CTGGCTGCCC TCCTGGGAAC CCAACAGCTG GGAGAAATTG TGACTCTGAT 
GCCACAAAAG CCAGCGAGGA TTTTGTGGAT CCATGGACGG TGCGGACAAG CAGTGCAAAA 
GGCATCGACT ATGACAAGCT CATTGTTCAG TTTGGAAGCA GCAAAATTGA CAAAGAGCTG 
ATAAACCGAA TAGAGCGAGC CACTGGCCAA AGACCGCACC GCTTCCTGCG CCGGGGCATC 
TTCTTCTCCC ACCGAGATAT GAATCAAATT CTGGATGCCT ATGAAAATAA GAAGCCATTC 
TACCTGTACA CAGGCAGGGG CCCCTCCTCC GAAGCAATGC ATCTGGGTCA TCTCGTCCCG 
TTTATTTTCA CAAAGTGGCT ACAGGATGTG TTCAATGTGC CTTTGGTCAT CCAGATGTCT 
GACGACGAGA AGTACCTGTG GAAGGACTTG ACTCTGGAGC AGGCATATAG CTACACCGTA 
GAAAATGCAA AGGACATCAT TGCCTGTGGC TTCGACATCA ACAAAACTTT CATCTTCTCT 
GATCTTGAGT ATATGGGGCA GAGCCCGGGC TTCTACAGGA ACGTGGTGAA GATTCAGAAG 
CACGTCACCT TCAACCAAGT GAAAGGCATT TTCGGCTTTA CGGACAGTGA CTGCATCGGG 
AAGATCAGTT TTCCTGCTGT CCAGGCCGCG CCATCGTTCA GCAACTCTTT CCCGAAGATC 
TTCCGAGACA GGACAGATAT CCAGTGCCTC ATCCCGTGTG CCATTGACCA GGATCCGTAC 
TTCAGAATGA CAAGGGACGT GGCCCCCAGG ATCGGCCATC CTAAACCTGC CCTGCTGCAC 
TCGACCTTCT TCCCTGCCCT GCAGGGCGCT CAGACCAAGA TGAGTGCCAG TGACCCCAAC 
TCCTCCATCT TCCTCACTGA CACGGCCAAG CAGATCAAGA GCAAGGTCAA CAAGCATGCA 
TTTTCTGGAG GAAGAGATAC TGTGGAGGAG CACAGGCAGT TTGGGGGCAA CTGTGAAGTG 
GATGTATCCT TCATGTATCT GACCTTCTTC CTTGAAGATG ACGACAGGCT GGAGCAGATC 
AGAAAGGATT ACACCAGCGG AGCCATGCTC ACCGGGGAGC TCAAGAAGAC GCTCATAGAC 
GTCCTTCAGC CCCTGATCGC AGAGCACCAG GCCCGACGCA AGGCGGTCAC TGAGGAGACC 
GTGAAGGAGT TCATGACGCC CCGGCAGCTG TCCTTCCACT TCCAGTGCTT CTGCTTTGAC 
ACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

Species-specific differences in the regulation of the aminoacylation activity of mammalian tryptophanyl-tRNA synthetases.
FEBS letters584(1)229-32(2010 Jan)
Wakasugi K

Tryptamine induces tryptophanyl-tRNA synthetase-mediated neurodegeneration with neurofibrillary tangles in human cell and mouse models.
Neuromolecular medicine9(1)55-82(2007)
Paley EL,Denisova G,Sokolova O,Posternak N,Wang X,Brownell AL

Aberrant regulation of imprinted gene expression in Gtl2lacZ mice.
Cytogenetic and genome research113(1-4)223-9(2006)
Sekita Y,Wagatsuma H,Irie M,Kobayashi S,Kohda T,Matsuda J,Yokoyama M,Ogura A,Schuster-Gossler K,Gossler A,Ishino F,Kaneko-Ishino T

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Wars cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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