Kdm6a gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
XM_006527595.4	XP_006527658.1	lysine-specific demethylase 6A isoform X2
XM_006527595.3	XP_006527658.1	lysine-specific demethylase 6A isoform X2
XM_017318461.1	XP_017173950.1	lysine-specific demethylase 6A isoform X3
XM_006527599.3	XP_006527662.1	lysine-specific demethylase 6A isoform X6
XM_006527597.4	XP_006527660.1	lysine-specific demethylase 6A isoform X5
XM_006527599.4	XP_006527662.1	lysine-specific demethylase 6A isoform X6
XM_006527596.3	XP_006527659.1	lysine-specific demethylase 6A isoform X4
XM_006527594.4	XP_006527657.1	lysine-specific demethylase 6A isoform X1
XM_006527596.4	XP_006527659.1	lysine-specific demethylase 6A isoform X4
NM_001310444.1	NP_001297373.1	lysine-specific demethylase 6A isoform 2
XM_006527594.3	XP_006527657.1	lysine-specific demethylase 6A isoform X1
XM_006527600.3	XP_006527663.1	lysine-specific demethylase 6A isoform X7
XM_006527600.4	XP_006527663.1	lysine-specific demethylase 6A isoform X7
NM_009483.2	NP_033509.1	lysine-specific demethylase 6A isoform 1
XM_006527597.3	XP_006527660.1	lysine-specific demethylase 6A isoform X5
XM_017318461.2	XP_017173950.1	lysine-specific demethylase 6A isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu17953	NM_009483.2 Latest version!	Mus musculus lysine (K)-specific demethylase 6A (Kdm6a), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OMu55891	XM_006527594.4 Latest version!	Mus musculus lysine (K)-specific demethylase 6A (Kdm6a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu55892	XM_006527595.4 Latest version!	Mus musculus lysine (K)-specific demethylase 6A (Kdm6a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu55893	XM_006527596.4 Latest version!	Mus musculus lysine (K)-specific demethylase 6A (Kdm6a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu55894	XM_006527597.4 Latest version!	Mus musculus lysine (K)-specific demethylase 6A (Kdm6a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu55896	XM_006527599.4 Latest version!	Mus musculus lysine (K)-specific demethylase 6A (Kdm6a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu55897	XM_006527600.4 Latest version!	Mus musculus lysine (K)-specific demethylase 6A (Kdm6a), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu83109	XM_017318461.2 Latest version!	Mus musculus lysine (K)-specific demethylase 6A (Kdm6a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu69025	NM_001310444.1 Latest version!	Mus musculus lysine (K)-specific demethylase 6A (Kdm6a), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
View Old Accession Versions >>
OMu55892	XM_006527595.3	Mus musculus lysine (K)-specific demethylase 6A (Kdm6a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OMu55896	XM_006527599.3	Mus musculus lysine (K)-specific demethylase 6A (Kdm6a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OMu55893	XM_006527596.3	Mus musculus lysine (K)-specific demethylase 6A (Kdm6a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OMu55891	XM_006527594.3	Mus musculus lysine (K)-specific demethylase 6A (Kdm6a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OMu55897	XM_006527600.3	Mus musculus lysine (K)-specific demethylase 6A (Kdm6a), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OMu55894	XM_006527597.3	Mus musculus lysine (K)-specific demethylase 6A (Kdm6a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OMu83109	XM_017318461.1	Mus musculus lysine (K)-specific demethylase 6A (Kdm6a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
Hide Old Accession Versions >>

Kdm6a ( NM_009483.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033509.1 Mus musculus lysine (K)-specific demethylase 6A (Kdm6a), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu17953
Clone ID Related Accession (Same CDS sequence)	NM_009483.2
Accession Version	NM_009483.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4275bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	lysine-specific demethylase 6A isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL773547.6, BC053433.1, AK164248.1 and AL732451.8. On Jul 14, 2015 this sequence version replaced NM_009483.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC053433.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END##

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCCG CCGCCGCCGC CGCCGCTTTC 
GGTGATGAGG AAAAGAAAAT GGCGGCGGGA AAAGCGAGCG GCGAGAGCGA GGAGGCGTCC 
CCCAGCCTGA CAGCGGAGGA GAGGGAGGCG CTCGGCGGAC TGGACAGCCG CCTTTTCGGG 
TTCGTGAGGT TTCATGAAGA TGGCGCCAGG ATGAAGGCCC TGCTGGGCAA GGCTGTTCGC 
TGCTACGAAT CTCTAATCTT AAAAGCTGAA GGGAAAGTGG AGTCTGATTT CTTTTGTCAA 
TTAGGTCACT TCAACCTCTT ATTGGAAGAT TATCCAAAAG CATTATCTGC ATACCAGAGG 
TACTACAGTT TACAGTCTGA TTACTGGAAG AATGCTGCCT TTTTATATGG TCTTGGTTTG 
GTCTACTTCC ATTACAATGC ATTTCAGTGG GCTATTAAAG CATTTCAGGA GGTGCTTTAT 
GTCGATCCCA GCTTTTGTCG AGCCAAGGAA ATTCATTTAC GACTTGGGCT TATGTTCAAA 
GTGAACACAG ACTATGAGTC TAGTTTAAAG CATTTTCAGT TAGCTTTGGT TGACTGTAAT 
CCCTGCACTT TGTCCAATGC TGAAATTCAG TTTCACATTG CCCACTTATA TGAAACCCAG 
AGGAAGTATC ATTCTGCAAA AGAAGCTTAT GAGCAACTTT TGCAGACAGA AAACCTTTCT 
GCACAAGTAA AAGCAACTAT TTTACAACAA TTAGGTTGGA TGCATCACAC TGTGGATCTC 
CTGGGAGATA AGGCCACCAA GGAAAGTTAT GCTATTCAGT ATCTCCAGAA GTCCTTGGAA 
GCAGATCCAA ATTCTGGCCA GTCCTGGTAT TTCCTTGGAA GGTGCTATTC AAGTATTGGG 
AAAGTTCAGG ATGCCTTTAT ATCTTACAGG CAATCTATTG ATAAATCAGA AGCAAGTGCA 
GATACATGGT GTTCAATAGG TGTGCTCTAT CAACAGCAAA ATCAGCCTAT GGATGCTTTG 
CAAGCTTATA TTTGTGCTGT ACAATTGGAC CACGGTCATG CTGCAGCCTG GATGGATCTA 
GGCACTCTCT ATGAATCCTG CAACCAACCT CAGGATGCTA TTAAATGCTA TTTAAATGCA 
ACTAGAAGCA AAAATTGTAG TAATACCTCT GGACTTGCAG CACGAATTAA GTATTTACAG 
GCTCAGTTGT GTAACCTTCC ACAAGGTAGT CTACAGAATA AAACTAAATT ACTTCCTAGT 
ATTGAGGAGG CATGGAGCCT ACCAATCCCC GCAGAGCTTA CCTCCAGGCA GGGTGCCATG 
AACACAGCAC AGCAGAATAC TTCTGATAAT TGGAGTGGTG GCAATGCACC ACCTCCAGTA 
GAACAACAAA CTCATTCATG GTGTTTGACA CCACAGAAAT TACAGCACTT GGAACAGCTC 
CGAGCAAACA GAAATAATTT AAATCCAGCA CAGAAACTAA TGCTGGAACA GCTGGAAAGT 
CAGTTTGTCT TAATGCAGCA ACACCAAATG AGACAAACAG GAGTTGCACA GGTACGGCCT 
ACTGGAATTC TTAATGGGCC AACAGTTGAC TCATCACTGC CTACAAACTC AGTTTCTGGC 
CAGCAGCCAC AGCTTCCTCT GACCAGAATG CCTAGTGTCT CTCAGCCTGG AGTCCACACT 
GCCTGTCCTA GGCAGACTTT GGCCAATGGA CCCTTTTCTG CAGGCCATGT TCCCTGTAGC 
ACATCAAGAA CACTGGGAAG TACAGACACT GTTTTGATAG GCAATAATCA TGTAACAGGA 
AGTGGAAGTA ATGGAAACGT GCCTTACCTG CAGCGAAACG CACCCACTCT ACCTCATAAC 
CGCACAAACC TGACCAGCAG CACAGAGGAG CCGTGGAAAA ACCAACTATC TAACTCCACT 
CAGGGGCTTC ACAAAGGTCC GAGTTCACAT TTGGCAGGTC CTAATGGTGA ACGACCTCTA 
TCTTCCACTG GGCCTTCCCA GCATCTCCAG GCAGCTGGCT CTGGTATTCA GAATCAGAAT 
GGACATCCCA CCCTGCCTAG CAATTCAGTA ACACAGGGGG CTGCTCTCAA TCACCTCTCC 
TCTCACACTG CTACCTCAGG TGGACAACAA GGCATTACCT TAACCAAAGA GAGCAAGCCT 
TCAGGAAACA CATTGACGGT GCCTGAAACA AGCAGGCAAA CTGGAGAGAC ACCTAACAGC 
ACTGCCAGTG TTGAGGGACT TCCTAATCAT GTCCATCAGG TGATGGCAGA TGCTGTTTGC 
AGTCCTAGCC ATGGAGATTC TAAGTCACCA GGTTTACTAA GTTCAGACAA TCCTCAGCTC 
TCTGCCTTGT TGATGGGAAA AGCTAATAAC AATGTGGGTC CTGGAACCTG TGACAAAGTC 
AATAACATCC ACCCAACTGT CCATACAAAG ACTGATAATT CTGTTGCCTC TTCACCATCT 
TCAGCCATTT CCACAGCAAC ACCTTCTCCT AAGTCCACTG AACAGACAAC CACAAACAGT 
GTTACCAGCC TTAACAGCCC TCACAGTGGG CTGCACACAA TTAATGGAGA AGGAATGGAA 
GAATCTCAGA GCCCCATTAA AACAGATCTG CTTCTAGTTA GCCACAGACC TAGTCCTCAG 
ATCATACCAT CAATGTCTGT GTCCATATAT CCCAGCTCAG CAGAAGTTCT GAAAGCTTGC 
AGGAATCTAG GTAAAAACGG CCTGTCTAAT AGTAGCATTC TGTTGGATAA ATGTCCGCCT 
CCAAGACCAC CATCCTCACC ATACCCTCCC TTGCCAAAGG ACAAGTTGAA TCCACCTACA 
CCTAGTATTT ATTTGGAAAA TAAACGTGAT GCTTTCTTTC CTCCATTACA TCAATTTTGT 
ACAAACCCAA ACAACCCTGT TACAGTAATA CGTGGCCTTG CTGGAGCTCT TAAATTAGAC 
TTGGGACTTT TCTCTACTAA AACTTTGGTG GAAGCTAACA ATGAACATAT GGTAGAAGTG 
AGGACACAGT TGTTACAACC AGCAGATGAA AATTGGGACC CTACTGGAAC CAAGAAAATC 
TGGCACTGTG AAAGTAATAG ATCTCATACT ACAATTGCTA AATATGCTCA GTACCAGGCC 
TCCTCATTCC AAGAATCATT GAGAGAAGAA AATGAGAAAA GAAGTCACCA TAAAGACCAC 
TCAGACAGTG AATCTACATC ATCAGATAAT TCTGGGAAAA GAAGAAAAGG ACCCTTTAAA 
ACCATTAAGT TTGGGACCAA CATTGACCTG TCTGATGACA AAAAGTGGAA GTTACAGCTA 
CATGAGCTGA CTAAACTTCC TGCCTTCGTG AGAGTTGTAT CTGCAGGAAA TCTTTTAAGC 
CACGTTGGTC ATACTATACT GGGCATGAAC ACAGTTCAAC TATACATGAA AGTTCCAGGA 
AGCAGAACAC CAGGTCATCA AGAAAATAAC AACTTCTGTT CAGTTAATAT AAATATTGGC 
CCAGGTGACT GTGAATGGTT TGTTGTTCCT GAAGGCTACT GGGGTGTTTT GAATGACTTC 
TGTGAAAAAA ATAATTTGAA TTTCTTAATG GGTTCTTGGT GGCCCAACCT TGAAGATCTA 
TATGAAGCAA ATGTTCCAGT GTATAGGTTT ATTCAGCGAC CTGGAGATCT GGTCTGGATA 
AATGCTGGCA CTGTTCATTG GGTTCAAGCT ATTGGCTGGT GCAACAATAT TGCTTGGAAT 
GTTGGTCCAC TTACAGCCTG TCAGTATAAG TTAGCAGTGG AACGTTATGA ATGGAACAAG 
TTGCAAAATG TAAAGTCAAT AGTACCCATG GTTCATCTTT CCTGGAATAT GGCACGAAAT 
ATCAAGGTTT CAGATCCAAA GCTTTTTGAA ATGATTAAGT ATTGTCTTCT GAGAACGCTG 
AAGCAATGTC AGACATTGAG GGAAGCTCTA ATTGCTGCAG GAAAAGAGAT CATATGGCAC 
GGGCGGACAA AAGAAGAACC AGCTCATTAT TGTAGTATTT GTGAGGTGGA GGTTTTTGAT 
CTGCTTTTTG TCACTAATGA GAGTAATTCT CGAAAAACCT ACATAGTACA TTGCCAAGAT 
TGTGCACGAA AAACAAGTGG GAATCTGGAA AATTTTGTGG TGCTAGAACA GTACAAAATG 
GAGGATCTGA TGCAAGTCTA TGACCAATTT ACATTAGTAA GTGAAATCAA CATGCTCCTC 
CATTACCATC CGCCTCATCT TGATATTGTT CCATGGACAT TAAACATGAG ACCTTTTCTG 
CTATTCAGAA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033509.1
CDS	432..4706
Translation	MKSCGVSLATAAAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARMKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATILQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKNCSNTSGLAARIKYLQAQLCNLPQGSLQNKTKLLPSIEEAWSLPIPAELTSRQGAMNTAQQNTSDNWSGGNAPPPVEQQTHSWCLTPQKLQHLEQLRANRNNLNPAQKLMLEQLESQFVLMQQHQMRQTGVAQVRPTGILNGPTVDSSLPTNSVSGQQPQLPLTRMPSVSQPGVHTACPRQTLANGPFSAGHVPCSTSRTLGSTDTVLIGNNHVTGSGSNGNVPYLQRNAPTLPHNRTNLTSSTEEPWKNQLSNSTQGLHKGPSSHLAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNTLTVPETSRQTGETPNSTASVEGLPNHVHQVMADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGPGTCDKVNNIHPTVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPIKTDLLLVSHRPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGKRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQNVKSIVPMVHLSWNMARNIKVSDPKLFEMIKYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLVSEINMLLHYHPPHLDIVPWTLNMRPFLLFRK

Target ORF information:

RefSeq Version	NM_009483.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus lysine (K)-specific demethylase 6A (Kdm6a), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009483.2

ORF Insert Sequence:

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCCG CCGCCGCCGC CGCCGCTTTC 
GGTGATGAGG AAAAGAAAAT GGCGGCGGGA AAAGCGAGCG GCGAGAGCGA GGAGGCGTCC 
CCCAGCCTGA CAGCGGAGGA GAGGGAGGCG CTCGGCGGAC TGGACAGCCG CCTTTTCGGG 
TTCGTGAGGT TTCATGAAGA TGGCGCCAGG ATGAAGGCCC TGCTGGGCAA GGCTGTTCGC 
TGCTACGAAT CTCTAATCTT AAAAGCTGAA GGGAAAGTGG AGTCTGATTT CTTTTGTCAA 
TTAGGTCACT TCAACCTCTT ATTGGAAGAT TATCCAAAAG CATTATCTGC ATACCAGAGG 
TACTACAGTT TACAGTCTGA TTACTGGAAG AATGCTGCCT TTTTATATGG TCTTGGTTTG 
GTCTACTTCC ATTACAATGC ATTTCAGTGG GCTATTAAAG CATTTCAGGA GGTGCTTTAT 
GTCGATCCCA GCTTTTGTCG AGCCAAGGAA ATTCATTTAC GACTTGGGCT TATGTTCAAA 
GTGAACACAG ACTATGAGTC TAGTTTAAAG CATTTTCAGT TAGCTTTGGT TGACTGTAAT 
CCCTGCACTT TGTCCAATGC TGAAATTCAG TTTCACATTG CCCACTTATA TGAAACCCAG 
AGGAAGTATC ATTCTGCAAA AGAAGCTTAT GAGCAACTTT TGCAGACAGA AAACCTTTCT 
GCACAAGTAA AAGCAACTAT TTTACAACAA TTAGGTTGGA TGCATCACAC TGTGGATCTC 
CTGGGAGATA AGGCCACCAA GGAAAGTTAT GCTATTCAGT ATCTCCAGAA GTCCTTGGAA 
GCAGATCCAA ATTCTGGCCA GTCCTGGTAT TTCCTTGGAA GGTGCTATTC AAGTATTGGG 
AAAGTTCAGG ATGCCTTTAT ATCTTACAGG CAATCTATTG ATAAATCAGA AGCAAGTGCA 
GATACATGGT GTTCAATAGG TGTGCTCTAT CAACAGCAAA ATCAGCCTAT GGATGCTTTG 
CAAGCTTATA TTTGTGCTGT ACAATTGGAC CACGGTCATG CTGCAGCCTG GATGGATCTA 
GGCACTCTCT ATGAATCCTG CAACCAACCT CAGGATGCTA TTAAATGCTA TTTAAATGCA 
ACTAGAAGCA AAAATTGTAG TAATACCTCT GGACTTGCAG CACGAATTAA GTATTTACAG 
GCTCAGTTGT GTAACCTTCC ACAAGGTAGT CTACAGAATA AAACTAAATT ACTTCCTAGT 
ATTGAGGAGG CATGGAGCCT ACCAATCCCC GCAGAGCTTA CCTCCAGGCA GGGTGCCATG 
AACACAGCAC AGCAGAATAC TTCTGATAAT TGGAGTGGTG GCAATGCACC ACCTCCAGTA 
GAACAACAAA CTCATTCATG GTGTTTGACA CCACAGAAAT TACAGCACTT GGAACAGCTC 
CGAGCAAACA GAAATAATTT AAATCCAGCA CAGAAACTAA TGCTGGAACA GCTGGAAAGT 
CAGTTTGTCT TAATGCAGCA ACACCAAATG AGACAAACAG GAGTTGCACA GGTACGGCCT 
ACTGGAATTC TTAATGGGCC AACAGTTGAC TCATCACTGC CTACAAACTC AGTTTCTGGC 
CAGCAGCCAC AGCTTCCTCT GACCAGAATG CCTAGTGTCT CTCAGCCTGG AGTCCACACT 
GCCTGTCCTA GGCAGACTTT GGCCAATGGA CCCTTTTCTG CAGGCCATGT TCCCTGTAGC 
ACATCAAGAA CACTGGGAAG TACAGACACT GTTTTGATAG GCAATAATCA TGTAACAGGA 
AGTGGAAGTA ATGGAAACGT GCCTTACCTG CAGCGAAACG CACCCACTCT ACCTCATAAC 
CGCACAAACC TGACCAGCAG CACAGAGGAG CCGTGGAAAA ACCAACTATC TAACTCCACT 
CAGGGGCTTC ACAAAGGTCC GAGTTCACAT TTGGCAGGTC CTAATGGTGA ACGACCTCTA 
TCTTCCACTG GGCCTTCCCA GCATCTCCAG GCAGCTGGCT CTGGTATTCA GAATCAGAAT 
GGACATCCCA CCCTGCCTAG CAATTCAGTA ACACAGGGGG CTGCTCTCAA TCACCTCTCC 
TCTCACACTG CTACCTCAGG TGGACAACAA GGCATTACCT TAACCAAAGA GAGCAAGCCT 
TCAGGAAACA CATTGACGGT GCCTGAAACA AGCAGGCAAA CTGGAGAGAC ACCTAACAGC 
ACTGCCAGTG TTGAGGGACT TCCTAATCAT GTCCATCAGG TGATGGCAGA TGCTGTTTGC 
AGTCCTAGCC ATGGAGATTC TAAGTCACCA GGTTTACTAA GTTCAGACAA TCCTCAGCTC 
TCTGCCTTGT TGATGGGAAA AGCTAATAAC AATGTGGGTC CTGGAACCTG TGACAAAGTC 
AATAACATCC ACCCAACTGT CCATACAAAG ACTGATAATT CTGTTGCCTC TTCACCATCT 
TCAGCCATTT CCACAGCAAC ACCTTCTCCT AAGTCCACTG AACAGACAAC CACAAACAGT 
GTTACCAGCC TTAACAGCCC TCACAGTGGG CTGCACACAA TTAATGGAGA AGGAATGGAA 
GAATCTCAGA GCCCCATTAA AACAGATCTG CTTCTAGTTA GCCACAGACC TAGTCCTCAG 
ATCATACCAT CAATGTCTGT GTCCATATAT CCCAGCTCAG CAGAAGTTCT GAAAGCTTGC 
AGGAATCTAG GTAAAAACGG CCTGTCTAAT AGTAGCATTC TGTTGGATAA ATGTCCGCCT 
CCAAGACCAC CATCCTCACC ATACCCTCCC TTGCCAAAGG ACAAGTTGAA TCCACCTACA 
CCTAGTATTT ATTTGGAAAA TAAACGTGAT GCTTTCTTTC CTCCATTACA TCAATTTTGT 
ACAAACCCAA ACAACCCTGT TACAGTAATA CGTGGCCTTG CTGGAGCTCT TAAATTAGAC 
TTGGGACTTT TCTCTACTAA AACTTTGGTG GAAGCTAACA ATGAACATAT GGTAGAAGTG 
AGGACACAGT TGTTACAACC AGCAGATGAA AATTGGGACC CTACTGGAAC CAAGAAAATC 
TGGCACTGTG AAAGTAATAG ATCTCATACT ACAATTGCTA AATATGCTCA GTACCAGGCC 
TCCTCATTCC AAGAATCATT GAGAGAAGAA AATGAGAAAA GAAGTCACCA TAAAGACCAC 
TCAGACAGTG AATCTACATC ATCAGATAAT TCTGGGAAAA GAAGAAAAGG ACCCTTTAAA 
ACCATTAAGT TTGGGACCAA CATTGACCTG TCTGATGACA AAAAGTGGAA GTTACAGCTA 
CATGAGCTGA CTAAACTTCC TGCCTTCGTG AGAGTTGTAT CTGCAGGAAA TCTTTTAAGC 
CACGTTGGTC ATACTATACT GGGCATGAAC ACAGTTCAAC TATACATGAA AGTTCCAGGA 
AGCAGAACAC CAGGTCATCA AGAAAATAAC AACTTCTGTT CAGTTAATAT AAATATTGGC 
CCAGGTGACT GTGAATGGTT TGTTGTTCCT GAAGGCTACT GGGGTGTTTT GAATGACTTC 
TGTGAAAAAA ATAATTTGAA TTTCTTAATG GGTTCTTGGT GGCCCAACCT TGAAGATCTA 
TATGAAGCAA ATGTTCCAGT GTATAGGTTT ATTCAGCGAC CTGGAGATCT GGTCTGGATA 
AATGCTGGCA CTGTTCATTG GGTTCAAGCT ATTGGCTGGT GCAACAATAT TGCTTGGAAT 
GTTGGTCCAC TTACAGCCTG TCAGTATAAG TTAGCAGTGG AACGTTATGA ATGGAACAAG 
TTGCAAAATG TAAAGTCAAT AGTACCCATG GTTCATCTTT CCTGGAATAT GGCACGAAAT 
ATCAAGGTTT CAGATCCAAA GCTTTTTGAA ATGATTAAGT ATTGTCTTCT GAGAACGCTG 
AAGCAATGTC AGACATTGAG GGAAGCTCTA ATTGCTGCAG GAAAAGAGAT CATATGGCAC 
GGGCGGACAA AAGAAGAACC AGCTCATTAT TGTAGTATTT GTGAGGTGGA GGTTTTTGAT 
CTGCTTTTTG TCACTAATGA GAGTAATTCT CGAAAAACCT ACATAGTACA TTGCCAAGAT 
TGTGCACGAA AAACAAGTGG GAATCTGGAA AATTTTGTGG TGCTAGAACA GTACAAAATG 
GAGGATCTGA TGCAAGTCTA TGACCAATTT ACATTAGTAA GTGAAATCAA CATGCTCCTC 
CATTACCATC CGCCTCATCT TGATATTGTT CCATGGACAT TAAACATGAG ACCTTTTCTG 
CTATTCAGAA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kdm6a ( XM_006527594.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527657.1 Mus musculus lysine (K)-specific demethylase 6A (Kdm6a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu55891
Clone ID Related Accession (Same CDS sequence)	XM_006527594.4 , XM_006527594.3
Accession Version	XM_006527594.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	lysine-specific demethylase 6A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006527594.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCCG CCGCCGCCGC CGCCGCTTTC 
GGTGATGAGG AAAAGAAAAT GGCGGCGGGA AAAGCGAGCG GCGAGAGCGA GGAGGCGTCC 
CCCAGCCTGA CAGCGGAGGA GAGGGAGGCG CTCGGCGGAC TGGACAGCCG CCTTTTCGGG 
TTCGTGAGGT TTCATGAAGA TGGCGCCAGG ATGAAGGCCC TGCTGGGCAA GGCTGTTCGC 
TGCTACGAAT CTCTAATCTT AAAAGCTGAA GGGAAAGTGG AGTCTGATTT CTTTTGTCAA 
TTAGGTCACT TCAACCTCTT ATTGGAAGAT TATCCAAAAG CATTATCTGC ATACCAGAGG 
TACTACAGTT TACAGTCTGA TTACTGGAAG AATGCTGCCT TTTTATATGG TCTTGGTTTG 
GTCTACTTCC ATTACAATGC ATTTCAGTGG GCTATTAAAG CATTTCAGGA GGTGCTTTAT 
GTCGATCCCA GCTTTTGTCG AGCCAAGGAA ATTCATTTAC GACTTGGGCT TATGTTCAAA 
GTGAACACAG ACTATGAGTC TAGTTTAAAG CATTTTCAGT TAGCTTTGGT TGACTGTAAT 
CCCTGCACTT TGTCCAATGC TGAAATTCAG TTTCACATTG CCCACTTATA TGAAACCCAG 
AGGAAGTATC ATTCTGCAAA AGAAGCTTAT GAGCAACTTT TGCAGACAGA AAACCTTTCT 
GCACAAGTAA AAGCAACTAT TTTACAACAA TTAGGTTGGA TGCATCACAC TGTGGATCTC 
CTGGGAGATA AGGCCACCAA GGAAAGTTAT GCTATTCAGT ATCTCCAGAA GTCCTTGGAA 
GCAGATCCAA ATTCTGGCCA GTCCTGGTAT TTCCTTGGAA GGTGCTATTC AAGTATTGGG 
AAAGTTCAGG ATGCCTTTAT ATCTTACAGG CAATCTATTG ATAAATCAGA AGCAAGTGCA 
GATACATGGT GTTCAATAGG TGTGCTCTAT CAACAGCAAA ATCAGCCTAT GGATGCTTTG 
CAAGCTTATA TTTGTGCTGT ACAATTGGAC CACGGTCATG CTGCAGCCTG GATGGATCTA 
GGCACTCTCT ATGAATCCTG CAACCAACCT CAGGATGCTA TTAAATGCTA TTTAAATGCA 
ACTAGAAGCA AAAATTGTAG TAATACCTCT GGACTTGCAG CACGAATTAA GTATTTACAG 
GCTCAGTTGT GTAACCTTCC ACAAGGTAGT CTACAGAATA AAACTAAATT ACTTCCTAGT 
ATTGAGGAGG CATGGAGCCT ACCAATCCCC GCAGAGCTTA CCTCCAGGCA GGGTGCCATG 
AACACAGCAC AGCAGGCATG TAAACCTCAT CATCCAAATA CTGAACCTGT ATTAGGCCTC 
AGTCAAACAC CAATTTCACA GCAGTCCTTG CCACTACACA TGATTCCTCC TAGCCAAGTA 
GATGACCTGT CCAGTCCTGC CAAGAGGAAA AGAACATCTA GTCCAACAAA GAATACTTCT 
GATAATTGGA GTGGTGGCAA TGCACCACCT CCAGTAGAAC AACAAACTCA TTCATGGTGT 
TTGACACCAC AGAAATTACA GCACTTGGAA CAGCTCCGAG CAAACAGAAA TAATTTAAAT 
CCAGCACAGA AACTAATGCT GGAACAGCTG GAAAGTCAGT TTGTCTTAAT GCAGCAACAC 
CAAATGAGAC AAACAGGAGT TGCACAGGTA CGGCCTACTG GAATTCTTAA TGGGCCAACA 
GTTGACTCAT CACTGCCTAC AAACTCAGTT TCTGGCCAGC AGCCACAGCT TCCTCTGACC 
AGAATGCCTA GTGTCTCTCA GCCTGGAGTC CACACTGCCT GTCCTAGGCA GACTTTGGCC 
AATGGACCCT TTTCTGCAGG CCATGTTCCC TGTAGCACAT CAAGAACACT GGGAAGTACA 
GACACTGTTT TGATAGGCAA TAATCATGTA ACAGGAAGTG GAAGTAATGG AAACGTGCCT 
TACCTGCAGC GAAACGCACC CACTCTACCT CATAACCGCA CAAACCTGAC CAGCAGCACA 
GAGGAGCCGT GGAAAAACCA ACTATCTAAC TCCACTCAGG GGCTTCACAA AGGTCCGAGT 
TCACATTTGG CAGGTCCTAA TGGTGAACGA CCTCTATCTT CCACTGGGCC TTCCCAGCAT 
CTCCAGGCAG CTGGCTCTGG TATTCAGAAT CAGAATGGAC ATCCCACCCT GCCTAGCAAT 
TCAGTAACAC AGGGGGCTGC TCTCAATCAC CTCTCCTCTC ACACTGCTAC CTCAGGTGGA 
CAACAAGGCA TTACCTTAAC CAAAGAGAGC AAGCCTTCAG GAAACACATT GACGGTGCCT 
GAAACAAGCA GGCAAACTGG AGAGACACCT AACAGCACTG CCAGTGTTGA GGGACTTCCT 
AATCATGTCC ATCAGGTGAT GGCAGATGCT GTTTGCAGTC CTAGCCATGG AGATTCTAAG 
TCACCAGGTT TACTAAGTTC AGACAATCCT CAGCTCTCTG CCTTGTTGAT GGGAAAAGCT 
AATAACAATG TGGGTCCTGG AACCTGTGAC AAAGTCAATA ACATCCACCC AACTGTCCAT 
ACAAAGACTG ATAATTCTGT TGCCTCTTCA CCATCTTCAG CCATTTCCAC AGCAACACCT 
TCTCCTAAGT CCACTGAACA GACAACCACA AACAGTGTTA CCAGCCTTAA CAGCCCTCAC 
AGTGGGCTGC ACACAATTAA TGGAGAAGGA ATGGAAGAAT CTCAGAGCCC CATTAAAACA 
GATCTGCTTC TAGTTAGCCA CAGACCTAGT CCTCAGATCA TACCATCAAT GTCTGTGTCC 
ATATATCCCA GCTCAGCAGA AGTTCTGAAA GCTTGCAGGA ATCTAGGTAA AAACGGCCTG 
TCTAATAGTA GCATTCTGTT GGATAAATGT CCGCCTCCAA GACCACCATC CTCACCATAC 
CCTCCCTTGC CAAAGGACAA GTTGAATCCA CCTACACCTA GTATTTATTT GGAAAATAAA 
CGTGATGCTT TCTTTCCTCC ATTACATCAA TTTTGTACAA ACCCAAACAA CCCTGTTACA 
GTAATACGTG GCCTTGCTGG AGCTCTTAAA TTAGACTTGG GACTTTTCTC TACTAAAACT 
TTGGTGGAAG CTAACAATGA ACATATGGTA GAAGTGAGGA CACAGTTGTT ACAACCAGCA 
GATGAAAATT GGGACCCTAC TGGAACCAAG AAAATCTGGC ACTGTGAAAG TAATAGATCT 
CATACTACAA TTGCTAAATA TGCTCAGTAC CAGGCCTCCT CATTCCAAGA ATCATTGAGA 
GAAGAAAATG AGAAAAGAAG TCACCATAAA GACCACTCAG ACAGTGAATC TACATCATCA 
GATAATTCTG GGAAAAGAAG AAAAGGACCC TTTAAAACCA TTAAGTTTGG GACCAACATT 
GACCTGTCTG ATGACAAAAA GTGGAAGTTA CAGCTACATG AGCTGACTAA ACTTCCTGCC 
TTCGTGAGAG TTGTATCTGC AGGAAATCTT TTAAGCCACG TTGGTCATAC TATACTGGGC 
ATGAACACAG TTCAACTATA CATGAAAGTT CCAGGAAGCA GAACACCAGG TCATCAAGAA 
AATAACAACT TCTGTTCAGT TAATATAAAT ATTGGCCCAG GTGACTGTGA ATGGTTTGTT 
GTTCCTGAAG GCTACTGGGG TGTTTTGAAT GACTTCTGTG AAAAAAATAA TTTGAATTTC 
TTAATGGGTT CTTGGTGGCC CAACCTTGAA GATCTATATG AAGCAAATGT TCCAGTGTAT 
AGGTTTATTC AGCGACCTGG AGATCTGGTC TGGATAAATG CTGGCACTGT TCATTGGGTT 
CAAGCTATTG GCTGGTGCAA CAATATTGCT TGGAATGTTG GTCCACTTAC AGCCTGTCAG 
TATAAGTTAG CAGTGGAACG TTATGAATGG AACAAGTTGC AAAATGTAAA GTCAATAGTA 
CCCATGGTTC ATCTTTCCTG GAATATGGCA CGAAATATCA AGGTTTCAGA TCCAAAGCTT 
TTTGAAATGA TTAAGTATTG TCTTCTGAGA ACGCTGAAGC AATGTCAGAC ATTGAGGGAA 
GCTCTAATTG CTGCAGGAAA AGAGATCATA TGGCACGGGC GGACAAAAGA AGAACCAGCT 
CATTATTGTA GTATTTGTGA GGTGGAGGTT TTTGATCTGC TTTTTGTCAC TAATGAGAGT 
AATTCTCGAA AAACCTACAT AGTACATTGC CAAGATTGTG CACGAAAAAC AAGTGGGAAT 
CTGGAAAATT TTGTGGTGCT AGAACAGTAC AAAATGGAGG ATCTGATGCA AGTCTATGAC 
CAATTTACAT TAGTAAGTGA AATCAACATG CTCCTCCATT ACCATCCGCC TCATCTTGAT 
ATTGTTCCAT GGACATTAAA CATGAGACCT TTTCTGCTAT TCAGAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006527657.1
CDS	2145..6575
Translation	MKSCGVSLATAAAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARMKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATILQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKNCSNTSGLAARIKYLQAQLCNLPQGSLQNKTKLLPSIEEAWSLPIPAELTSRQGAMNTAQQACKPHHPNTEPVLGLSQTPISQQSLPLHMIPPSQVDDLSSPAKRKRTSSPTKNTSDNWSGGNAPPPVEQQTHSWCLTPQKLQHLEQLRANRNNLNPAQKLMLEQLESQFVLMQQHQMRQTGVAQVRPTGILNGPTVDSSLPTNSVSGQQPQLPLTRMPSVSQPGVHTACPRQTLANGPFSAGHVPCSTSRTLGSTDTVLIGNNHVTGSGSNGNVPYLQRNAPTLPHNRTNLTSSTEEPWKNQLSNSTQGLHKGPSSHLAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNTLTVPETSRQTGETPNSTASVEGLPNHVHQVMADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGPGTCDKVNNIHPTVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPIKTDLLLVSHRPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGKRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQNVKSIVPMVHLSWNMARNIKVSDPKLFEMIKYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLVSEINMLLHYHPPHLDIVPWTLNMRPFLLFRK

Target ORF information:

RefSeq Version	XM_006527594.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus lysine (K)-specific demethylase 6A (Kdm6a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527594.4

ORF Insert Sequence:

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCCG CCGCCGCCGC CGCCGCTTTC 
GGTGATGAGG AAAAGAAAAT GGCGGCGGGA AAAGCGAGCG GCGAGAGCGA GGAGGCGTCC 
CCCAGCCTGA CAGCGGAGGA GAGGGAGGCG CTCGGCGGAC TGGACAGCCG CCTTTTCGGG 
TTCGTGAGGT TTCATGAAGA TGGCGCCAGG ATGAAGGCCC TGCTGGGCAA GGCTGTTCGC 
TGCTACGAAT CTCTAATCTT AAAAGCTGAA GGGAAAGTGG AGTCTGATTT CTTTTGTCAA 
TTAGGTCACT TCAACCTCTT ATTGGAAGAT TATCCAAAAG CATTATCTGC ATACCAGAGG 
TACTACAGTT TACAGTCTGA TTACTGGAAG AATGCTGCCT TTTTATATGG TCTTGGTTTG 
GTCTACTTCC ATTACAATGC ATTTCAGTGG GCTATTAAAG CATTTCAGGA GGTGCTTTAT 
GTCGATCCCA GCTTTTGTCG AGCCAAGGAA ATTCATTTAC GACTTGGGCT TATGTTCAAA 
GTGAACACAG ACTATGAGTC TAGTTTAAAG CATTTTCAGT TAGCTTTGGT TGACTGTAAT 
CCCTGCACTT TGTCCAATGC TGAAATTCAG TTTCACATTG CCCACTTATA TGAAACCCAG 
AGGAAGTATC ATTCTGCAAA AGAAGCTTAT GAGCAACTTT TGCAGACAGA AAACCTTTCT 
GCACAAGTAA AAGCAACTAT TTTACAACAA TTAGGTTGGA TGCATCACAC TGTGGATCTC 
CTGGGAGATA AGGCCACCAA GGAAAGTTAT GCTATTCAGT ATCTCCAGAA GTCCTTGGAA 
GCAGATCCAA ATTCTGGCCA GTCCTGGTAT TTCCTTGGAA GGTGCTATTC AAGTATTGGG 
AAAGTTCAGG ATGCCTTTAT ATCTTACAGG CAATCTATTG ATAAATCAGA AGCAAGTGCA 
GATACATGGT GTTCAATAGG TGTGCTCTAT CAACAGCAAA ATCAGCCTAT GGATGCTTTG 
CAAGCTTATA TTTGTGCTGT ACAATTGGAC CACGGTCATG CTGCAGCCTG GATGGATCTA 
GGCACTCTCT ATGAATCCTG CAACCAACCT CAGGATGCTA TTAAATGCTA TTTAAATGCA 
ACTAGAAGCA AAAATTGTAG TAATACCTCT GGACTTGCAG CACGAATTAA GTATTTACAG 
GCTCAGTTGT GTAACCTTCC ACAAGGTAGT CTACAGAATA AAACTAAATT ACTTCCTAGT 
ATTGAGGAGG CATGGAGCCT ACCAATCCCC GCAGAGCTTA CCTCCAGGCA GGGTGCCATG 
AACACAGCAC AGCAGGCATG TAAACCTCAT CATCCAAATA CTGAACCTGT ATTAGGCCTC 
AGTCAAACAC CAATTTCACA GCAGTCCTTG CCACTACACA TGATTCCTCC TAGCCAAGTA 
GATGACCTGT CCAGTCCTGC CAAGAGGAAA AGAACATCTA GTCCAACAAA GAATACTTCT 
GATAATTGGA GTGGTGGCAA TGCACCACCT CCAGTAGAAC AACAAACTCA TTCATGGTGT 
TTGACACCAC AGAAATTACA GCACTTGGAA CAGCTCCGAG CAAACAGAAA TAATTTAAAT 
CCAGCACAGA AACTAATGCT GGAACAGCTG GAAAGTCAGT TTGTCTTAAT GCAGCAACAC 
CAAATGAGAC AAACAGGAGT TGCACAGGTA CGGCCTACTG GAATTCTTAA TGGGCCAACA 
GTTGACTCAT CACTGCCTAC AAACTCAGTT TCTGGCCAGC AGCCACAGCT TCCTCTGACC 
AGAATGCCTA GTGTCTCTCA GCCTGGAGTC CACACTGCCT GTCCTAGGCA GACTTTGGCC 
AATGGACCCT TTTCTGCAGG CCATGTTCCC TGTAGCACAT CAAGAACACT GGGAAGTACA 
GACACTGTTT TGATAGGCAA TAATCATGTA ACAGGAAGTG GAAGTAATGG AAACGTGCCT 
TACCTGCAGC GAAACGCACC CACTCTACCT CATAACCGCA CAAACCTGAC CAGCAGCACA 
GAGGAGCCGT GGAAAAACCA ACTATCTAAC TCCACTCAGG GGCTTCACAA AGGTCCGAGT 
TCACATTTGG CAGGTCCTAA TGGTGAACGA CCTCTATCTT CCACTGGGCC TTCCCAGCAT 
CTCCAGGCAG CTGGCTCTGG TATTCAGAAT CAGAATGGAC ATCCCACCCT GCCTAGCAAT 
TCAGTAACAC AGGGGGCTGC TCTCAATCAC CTCTCCTCTC ACACTGCTAC CTCAGGTGGA 
CAACAAGGCA TTACCTTAAC CAAAGAGAGC AAGCCTTCAG GAAACACATT GACGGTGCCT 
GAAACAAGCA GGCAAACTGG AGAGACACCT AACAGCACTG CCAGTGTTGA GGGACTTCCT 
AATCATGTCC ATCAGGTGAT GGCAGATGCT GTTTGCAGTC CTAGCCATGG AGATTCTAAG 
TCACCAGGTT TACTAAGTTC AGACAATCCT CAGCTCTCTG CCTTGTTGAT GGGAAAAGCT 
AATAACAATG TGGGTCCTGG AACCTGTGAC AAAGTCAATA ACATCCACCC AACTGTCCAT 
ACAAAGACTG ATAATTCTGT TGCCTCTTCA CCATCTTCAG CCATTTCCAC AGCAACACCT 
TCTCCTAAGT CCACTGAACA GACAACCACA AACAGTGTTA CCAGCCTTAA CAGCCCTCAC 
AGTGGGCTGC ACACAATTAA TGGAGAAGGA ATGGAAGAAT CTCAGAGCCC CATTAAAACA 
GATCTGCTTC TAGTTAGCCA CAGACCTAGT CCTCAGATCA TACCATCAAT GTCTGTGTCC 
ATATATCCCA GCTCAGCAGA AGTTCTGAAA GCTTGCAGGA ATCTAGGTAA AAACGGCCTG 
TCTAATAGTA GCATTCTGTT GGATAAATGT CCGCCTCCAA GACCACCATC CTCACCATAC 
CCTCCCTTGC CAAAGGACAA GTTGAATCCA CCTACACCTA GTATTTATTT GGAAAATAAA 
CGTGATGCTT TCTTTCCTCC ATTACATCAA TTTTGTACAA ACCCAAACAA CCCTGTTACA 
GTAATACGTG GCCTTGCTGG AGCTCTTAAA TTAGACTTGG GACTTTTCTC TACTAAAACT 
TTGGTGGAAG CTAACAATGA ACATATGGTA GAAGTGAGGA CACAGTTGTT ACAACCAGCA 
GATGAAAATT GGGACCCTAC TGGAACCAAG AAAATCTGGC ACTGTGAAAG TAATAGATCT 
CATACTACAA TTGCTAAATA TGCTCAGTAC CAGGCCTCCT CATTCCAAGA ATCATTGAGA 
GAAGAAAATG AGAAAAGAAG TCACCATAAA GACCACTCAG ACAGTGAATC TACATCATCA 
GATAATTCTG GGAAAAGAAG AAAAGGACCC TTTAAAACCA TTAAGTTTGG GACCAACATT 
GACCTGTCTG ATGACAAAAA GTGGAAGTTA CAGCTACATG AGCTGACTAA ACTTCCTGCC 
TTCGTGAGAG TTGTATCTGC AGGAAATCTT TTAAGCCACG TTGGTCATAC TATACTGGGC 
ATGAACACAG TTCAACTATA CATGAAAGTT CCAGGAAGCA GAACACCAGG TCATCAAGAA 
AATAACAACT TCTGTTCAGT TAATATAAAT ATTGGCCCAG GTGACTGTGA ATGGTTTGTT 
GTTCCTGAAG GCTACTGGGG TGTTTTGAAT GACTTCTGTG AAAAAAATAA TTTGAATTTC 
TTAATGGGTT CTTGGTGGCC CAACCTTGAA GATCTATATG AAGCAAATGT TCCAGTGTAT 
AGGTTTATTC AGCGACCTGG AGATCTGGTC TGGATAAATG CTGGCACTGT TCATTGGGTT 
CAAGCTATTG GCTGGTGCAA CAATATTGCT TGGAATGTTG GTCCACTTAC AGCCTGTCAG 
TATAAGTTAG CAGTGGAACG TTATGAATGG AACAAGTTGC AAAATGTAAA GTCAATAGTA 
CCCATGGTTC ATCTTTCCTG GAATATGGCA CGAAATATCA AGGTTTCAGA TCCAAAGCTT 
TTTGAAATGA TTAAGTATTG TCTTCTGAGA ACGCTGAAGC AATGTCAGAC ATTGAGGGAA 
GCTCTAATTG CTGCAGGAAA AGAGATCATA TGGCACGGGC GGACAAAAGA AGAACCAGCT 
CATTATTGTA GTATTTGTGA GGTGGAGGTT TTTGATCTGC TTTTTGTCAC TAATGAGAGT 
AATTCTCGAA AAACCTACAT AGTACATTGC CAAGATTGTG CACGAAAAAC AAGTGGGAAT 
CTGGAAAATT TTGTGGTGCT AGAACAGTAC AAAATGGAGG ATCTGATGCA AGTCTATGAC 
CAATTTACAT TAGTAAGTGA AATCAACATG CTCCTCCATT ACCATCCGCC TCATCTTGAT 
ATTGTTCCAT GGACATTAAA CATGAGACCT TTTCTGCTAT TCAGAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kdm6a ( XM_006527595.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527658.1 Mus musculus lysine (K)-specific demethylase 6A (Kdm6a), transcript variant X2, mRNA.

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CloneID	OMu55892
Clone ID Related Accession (Same CDS sequence)	XM_006527595.4 , XM_006527595.3
Accession Version	XM_006527595.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4362bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	lysine-specific demethylase 6A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006527595.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCCG CCGCCGCCGC CGCCGCTTTC 
GGTGATGAGG AAAAGAAAAT GGCGGCGGGA AAAGCGAGCG GCGAGAGCGA GGAGGCGTCC 
CCCAGCCTGA CAGCGGAGGA GAGGGAGGCG CTCGGCGGAC TGGACAGCCG CCTTTTCGGG 
TTCGTGAGGT TTCATGAAGA TGGCGCCAGG ATGAAGGCCC TGCTGGGCAA GGCTGTTCGC 
TGCTACGAAT CTCTAATCTT AAAAGCTGAA GGGAAAGTGG AGTCTGATTT CTTTTGTCAA 
TTAGGTCACT TCAACCTCTT ATTGGAAGAT TATCCAAAAG CATTATCTGC ATACCAGAGG 
TACTACAGTT TACAGTCTGA TTACTGGAAG AATGCTGCCT TTTTATATGG TCTTGGTTTG 
GTCTACTTCC ATTACAATGC ATTTCAGTGG GCTATTAAAG CATTTCAGGA GGTGCTTTAT 
GTCGATCCCA GCTTTTGTCG AGCCAAGGAA ATTCATTTAC GACTTGGGCT TATGTTCAAA 
GTGAACACAG ACTATGAGTC TAGTTTAAAG CATTTTCAGT TAGCTTTGGT TGACTGTAAT 
CCCTGCACTT TGTCCAATGC TGAAATTCAG TTTCACATTG CCCACTTATA TGAAACCCAG 
AGGAAGTATC ATTCTGCAAA AGAAGCTTAT GAGCAACTTT TGCAGACAGA AAACCTTTCT 
GCACAAGTAA AAGCAACTAT TTTACAACAA TTAGGTTGGA TGCATCACAC TGTGGATCTC 
CTGGGAGATA AGGCCACCAA GGAAAGTTAT GCTATTCAGT ATCTCCAGAA GTCCTTGGAA 
GCAGATCCAA ATTCTGGCCA GTCCTGGTAT TTCCTTGGAA GGTGCTATTC AAGTATTGGG 
AAAGTTCAGG ATGCCTTTAT ATCTTACAGG CAATCTATTG ATAAATCAGA AGCAAGTGCA 
GATACATGGT GTTCAATAGG TGTGCTCTAT CAACAGCAAA ATCAGCCTAT GGATGCTTTG 
CAAGCTTATA TTTGTGCTGT ACAATTGGAC CACGGTCATG CTGCAGCCTG GATGGATCTA 
GGCACTCTCT ATGAATCCTG CAACCAACCT CAGGATGCTA TTAAATGCTA TTTAAATGCA 
ACTAGAAGCA AAAATTGTAG TAATACCTCT GGACTTGCAG CACGAATTAA GTATTTACAG 
GCTCAGTTGT GTAACCTTCC ACAAGGTAGT CTACAGAATA AAACTAAATT ACTTCCTAGT 
ATTGAGGAGG CATGGAGCCT ACCAATCCCC GCAGAGCTTA CCTCCAGGCA GGGTGCCATG 
AACACAGCAC AGCAGGCATG TAAACCTCAT CATCCAAATA CTGAACCTGT ATTAGGCCTC 
AGTCAAACAC CAATTTCACA GCAGTCCTTG CCACTACACA TGATTCCTCC TAGCCAAGTA 
GATGACCTGT CCAGTCCTGC CAAGAGGAAA AGAACATCTA GTCCAACAAA GAATACTTCT 
GATAATTGGA GTGGTGGCAA TGCACCACCT CCAGTAGAAC AACAAACTCA TTCATGGTGT 
TTGACACCAC AGAAATTACA GCACTTGGAA CAGCTCCGAG CAAACAGAAA TAATTTAAAT 
CCAGCACAGA AACTAATGCT GGAACAGCTG GAAAGTCAGT TTGTCTTAAT GCAGCAACAC 
CAAATGAGAC AAACAGGAGT TGCACAGGTA CGGCCTACTG GAATTCTTAA TGGGCCAACA 
GTTGACTCAT CACTGCCTAC AAACTCAGTT TCTGGCCAGC AGCCACAGCT TCCTCTGACC 
AGAATGCCTA GTGTCTCTCA GCCTGGAGTC CACACTGCCT GTCCTAGGCA GACTTTGGCC 
AATGGACCCT TTTCTGCAGG CCATGTTCCC TGTAGCACAT CAAGAACACT GGGAAGTACA 
GACACTGTTT TGATAGGCAA TAATCATGTA ACAGGAAGTG GAAGTAATGG AAACGTGCCT 
TACCTGCAGC GAAACGCACC CACTCTACCT CATAACCGCA CAAACCTGAC CAGCAGCACA 
GAGGAGCCGT GGAAAAACCA ACTATCTAAC TCCACTCAGG GGCTTCACAA AGGTCCGAGT 
TCACATTTGG CAGGTCCTAA TGGTGAACGA CCTCTATCTT CCACTGGGCC TTCCCAGCAT 
CTCCAGGCAG CTGGCTCTGG TATTCAGAAT CAGAATGGAC ATCCCACCCT GCCTAGCAAT 
TCAGTAACAC AGGGGGCTGC TCTCAATCAC CTCTCCTCTC ACACTGCTAC CTCAGGTGGA 
CAACAAGGCA TTACCTTAAC CAAAGAGAGC AAGCCTTCAG GAAACACATT GACGGTGCCT 
GAAACAAGCA GGCAAACTGG AGAGACACCT AACAGCACTG CCAGTGTTGA GGGACTTCCT 
AATCATGTCC ATCAGGTGAT GGCAGATGCT GTTTGCAGTC CTAGCCATGG AGATTCTAAG 
TCACCAGGTT TACTAAGTTC AGACAATCCT CAGCTCTCTG CCTTGTTGAT GGGAAAAGCT 
AATAACAATG TGGGTCCTGG AACCTGTGAC AAAGTCAATA ACATCCACCC AACTGTCCAT 
ACAAAGACTG ATAATTCTGT TGCCTCTTCA CCATCTTCAG CCATTTCCAC AGCAACACCT 
TCTCCTAAGT CCACTGAACA GACAACCACA AACAGTGTTA CCAGCCTTAA CAGCCCTCAC 
AGTGGGCTGC ACACAATTAA TGGAGAAGGA ATGGAAGAAT CTCAGAGCCC CATTAAAACA 
GATCTGCTTC TAGTTAGCCA CAGACCTAGT CCTCAGATCA TACCATCAAT GTCTGTGTCC 
ATATATCCCA GCTCAGCAGA AGTTCTGAAA GCTTGCAGGA ATCTAGGTAA AAACGGCCTG 
TCTAATAGTA GCATTCTGTT GGATAAATGT CCGCCTCCAA GACCACCATC CTCACCATAC 
CCTCCCTTGC CAAAGGACAA GTTGAATCCA CCTACACCTA GTATTTATTT GGAAAATAAA 
CGTGATGCTT TCTTTCCTCC ATTACATCAA TTTTGTACAA ACCCAAACAA CCCTGTTACA 
GTAATACGTG GCCTTGCTGG AGCTCTTAAA TTAGACTTGG GACTTTTCTC TACTAAAACT 
TTGGTGGAAG CTAACAATGA ACATATGGTA GAAGTGAGGA CACAGTTGTT ACAACCAGCA 
GATGAAAATT GGGACCCTAC TGGAACCAAG AAAATCTGGC ACTGTGAAAG TAATAGATCT 
CATACTACAA TTGCTAAATA TGCTCAGTAC CAGGCCTCCT CATTCCAAGA ATCATTGAGA 
GAAGAAAATG AGAAAAGAAG TCACCATAAA GACCACTCAG ACAGTGAATC TACATCATCA 
GATAATTCTG GGAAAAGAAG AAAAGGACCC TTTAAAACCA TTAAGTTTGG GACCAACATT 
GACCTGTCTG ATGACAAAAA GTGGAAGTTA CAGCTACATG AGCTGACTAA ACTTCCTGCC 
TTCGTGAGAG TTGTATCTGC AGGAAATCTT TTAAGCCACG TTGGTCATAC TATACTGGGC 
ATGAACACAG TTCAACTATA CATGAAAGTT CCAGGAAGCA GAACACCAGG TCATCAAGAA 
AATAACAACT TCTGTTCAGT TAATATAAAT ATTGGCCCAG GTGACTGTGA ATGGTTTGTT 
GTTCCTGAAG GCTACTGGGG TGTTTTGAAT GACTTCTGTG AAAAAAATAA TTTGAATTTC 
TTAATGGGTT CTTGGTGGCC CAACCTTGAA GATCTATATG AAGCAAATGT TCCAGTGTAT 
AGGTTTATTC AGCGACCTGG AGATCTGGTC TGGATAAATG CTGGCACTGT TCATTGGGTT 
CAAGCTATTG GCTGGTGCAA CAATATTGCT TGGAATGTTG GTCCACTTAC AGCCTGTCAG 
TATAAGTTAG CAGTGGAACG TTATGAATGG AACAAGTTGC AAAATGTAAA GTCAATAGTA 
CCCATGGTTC ATCTTTCCTG GAATATGGCA CGAAATATCA AGGTTTCAGA TCCAAAGCTT 
TTTGAAATGA TTAAGTATTG TCTTCTGAGA ACGCTGAAGC AATGTCAGAC ATTGAGGGAA 
GCTCTAATTG CTGCAGGAAA AGAGATCATA TGGCACGGGC GGACAAAAGA AGAACCAGCT 
CATTATTGTA GTATTTGTGA GGTGGAGGTT TTTGATCTGC TTTTTGTCAC TAATGAGAGT 
AATTCTCGAA AAACCTACAT AGTACATTGC CAAGATTGTG CACGAAAAAC AAGTGGGAAT 
CTGGAAAATT TTGTGGTGCT AGAACAGTAC AAAATGGAGG ATCTGATGCA AGTCTATGAC 
CAATTTACAT TAGCTCCTCC ATTACCATCC GCCTCATCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006527658.1
CDS	2145..6506
Translation	MKSCGVSLATAAAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARMKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATILQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKNCSNTSGLAARIKYLQAQLCNLPQGSLQNKTKLLPSIEEAWSLPIPAELTSRQGAMNTAQQACKPHHPNTEPVLGLSQTPISQQSLPLHMIPPSQVDDLSSPAKRKRTSSPTKNTSDNWSGGNAPPPVEQQTHSWCLTPQKLQHLEQLRANRNNLNPAQKLMLEQLESQFVLMQQHQMRQTGVAQVRPTGILNGPTVDSSLPTNSVSGQQPQLPLTRMPSVSQPGVHTACPRQTLANGPFSAGHVPCSTSRTLGSTDTVLIGNNHVTGSGSNGNVPYLQRNAPTLPHNRTNLTSSTEEPWKNQLSNSTQGLHKGPSSHLAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNTLTVPETSRQTGETPNSTASVEGLPNHVHQVMADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGPGTCDKVNNIHPTVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPIKTDLLLVSHRPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGKRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQNVKSIVPMVHLSWNMARNIKVSDPKLFEMIKYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLAPPLPSASS

Target ORF information:

RefSeq Version	XM_006527595.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus lysine (K)-specific demethylase 6A (Kdm6a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527595.4

ORF Insert Sequence:

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCCG CCGCCGCCGC CGCCGCTTTC 
GGTGATGAGG AAAAGAAAAT GGCGGCGGGA AAAGCGAGCG GCGAGAGCGA GGAGGCGTCC 
CCCAGCCTGA CAGCGGAGGA GAGGGAGGCG CTCGGCGGAC TGGACAGCCG CCTTTTCGGG 
TTCGTGAGGT TTCATGAAGA TGGCGCCAGG ATGAAGGCCC TGCTGGGCAA GGCTGTTCGC 
TGCTACGAAT CTCTAATCTT AAAAGCTGAA GGGAAAGTGG AGTCTGATTT CTTTTGTCAA 
TTAGGTCACT TCAACCTCTT ATTGGAAGAT TATCCAAAAG CATTATCTGC ATACCAGAGG 
TACTACAGTT TACAGTCTGA TTACTGGAAG AATGCTGCCT TTTTATATGG TCTTGGTTTG 
GTCTACTTCC ATTACAATGC ATTTCAGTGG GCTATTAAAG CATTTCAGGA GGTGCTTTAT 
GTCGATCCCA GCTTTTGTCG AGCCAAGGAA ATTCATTTAC GACTTGGGCT TATGTTCAAA 
GTGAACACAG ACTATGAGTC TAGTTTAAAG CATTTTCAGT TAGCTTTGGT TGACTGTAAT 
CCCTGCACTT TGTCCAATGC TGAAATTCAG TTTCACATTG CCCACTTATA TGAAACCCAG 
AGGAAGTATC ATTCTGCAAA AGAAGCTTAT GAGCAACTTT TGCAGACAGA AAACCTTTCT 
GCACAAGTAA AAGCAACTAT TTTACAACAA TTAGGTTGGA TGCATCACAC TGTGGATCTC 
CTGGGAGATA AGGCCACCAA GGAAAGTTAT GCTATTCAGT ATCTCCAGAA GTCCTTGGAA 
GCAGATCCAA ATTCTGGCCA GTCCTGGTAT TTCCTTGGAA GGTGCTATTC AAGTATTGGG 
AAAGTTCAGG ATGCCTTTAT ATCTTACAGG CAATCTATTG ATAAATCAGA AGCAAGTGCA 
GATACATGGT GTTCAATAGG TGTGCTCTAT CAACAGCAAA ATCAGCCTAT GGATGCTTTG 
CAAGCTTATA TTTGTGCTGT ACAATTGGAC CACGGTCATG CTGCAGCCTG GATGGATCTA 
GGCACTCTCT ATGAATCCTG CAACCAACCT CAGGATGCTA TTAAATGCTA TTTAAATGCA 
ACTAGAAGCA AAAATTGTAG TAATACCTCT GGACTTGCAG CACGAATTAA GTATTTACAG 
GCTCAGTTGT GTAACCTTCC ACAAGGTAGT CTACAGAATA AAACTAAATT ACTTCCTAGT 
ATTGAGGAGG CATGGAGCCT ACCAATCCCC GCAGAGCTTA CCTCCAGGCA GGGTGCCATG 
AACACAGCAC AGCAGGCATG TAAACCTCAT CATCCAAATA CTGAACCTGT ATTAGGCCTC 
AGTCAAACAC CAATTTCACA GCAGTCCTTG CCACTACACA TGATTCCTCC TAGCCAAGTA 
GATGACCTGT CCAGTCCTGC CAAGAGGAAA AGAACATCTA GTCCAACAAA GAATACTTCT 
GATAATTGGA GTGGTGGCAA TGCACCACCT CCAGTAGAAC AACAAACTCA TTCATGGTGT 
TTGACACCAC AGAAATTACA GCACTTGGAA CAGCTCCGAG CAAACAGAAA TAATTTAAAT 
CCAGCACAGA AACTAATGCT GGAACAGCTG GAAAGTCAGT TTGTCTTAAT GCAGCAACAC 
CAAATGAGAC AAACAGGAGT TGCACAGGTA CGGCCTACTG GAATTCTTAA TGGGCCAACA 
GTTGACTCAT CACTGCCTAC AAACTCAGTT TCTGGCCAGC AGCCACAGCT TCCTCTGACC 
AGAATGCCTA GTGTCTCTCA GCCTGGAGTC CACACTGCCT GTCCTAGGCA GACTTTGGCC 
AATGGACCCT TTTCTGCAGG CCATGTTCCC TGTAGCACAT CAAGAACACT GGGAAGTACA 
GACACTGTTT TGATAGGCAA TAATCATGTA ACAGGAAGTG GAAGTAATGG AAACGTGCCT 
TACCTGCAGC GAAACGCACC CACTCTACCT CATAACCGCA CAAACCTGAC CAGCAGCACA 
GAGGAGCCGT GGAAAAACCA ACTATCTAAC TCCACTCAGG GGCTTCACAA AGGTCCGAGT 
TCACATTTGG CAGGTCCTAA TGGTGAACGA CCTCTATCTT CCACTGGGCC TTCCCAGCAT 
CTCCAGGCAG CTGGCTCTGG TATTCAGAAT CAGAATGGAC ATCCCACCCT GCCTAGCAAT 
TCAGTAACAC AGGGGGCTGC TCTCAATCAC CTCTCCTCTC ACACTGCTAC CTCAGGTGGA 
CAACAAGGCA TTACCTTAAC CAAAGAGAGC AAGCCTTCAG GAAACACATT GACGGTGCCT 
GAAACAAGCA GGCAAACTGG AGAGACACCT AACAGCACTG CCAGTGTTGA GGGACTTCCT 
AATCATGTCC ATCAGGTGAT GGCAGATGCT GTTTGCAGTC CTAGCCATGG AGATTCTAAG 
TCACCAGGTT TACTAAGTTC AGACAATCCT CAGCTCTCTG CCTTGTTGAT GGGAAAAGCT 
AATAACAATG TGGGTCCTGG AACCTGTGAC AAAGTCAATA ACATCCACCC AACTGTCCAT 
ACAAAGACTG ATAATTCTGT TGCCTCTTCA CCATCTTCAG CCATTTCCAC AGCAACACCT 
TCTCCTAAGT CCACTGAACA GACAACCACA AACAGTGTTA CCAGCCTTAA CAGCCCTCAC 
AGTGGGCTGC ACACAATTAA TGGAGAAGGA ATGGAAGAAT CTCAGAGCCC CATTAAAACA 
GATCTGCTTC TAGTTAGCCA CAGACCTAGT CCTCAGATCA TACCATCAAT GTCTGTGTCC 
ATATATCCCA GCTCAGCAGA AGTTCTGAAA GCTTGCAGGA ATCTAGGTAA AAACGGCCTG 
TCTAATAGTA GCATTCTGTT GGATAAATGT CCGCCTCCAA GACCACCATC CTCACCATAC 
CCTCCCTTGC CAAAGGACAA GTTGAATCCA CCTACACCTA GTATTTATTT GGAAAATAAA 
CGTGATGCTT TCTTTCCTCC ATTACATCAA TTTTGTACAA ACCCAAACAA CCCTGTTACA 
GTAATACGTG GCCTTGCTGG AGCTCTTAAA TTAGACTTGG GACTTTTCTC TACTAAAACT 
TTGGTGGAAG CTAACAATGA ACATATGGTA GAAGTGAGGA CACAGTTGTT ACAACCAGCA 
GATGAAAATT GGGACCCTAC TGGAACCAAG AAAATCTGGC ACTGTGAAAG TAATAGATCT 
CATACTACAA TTGCTAAATA TGCTCAGTAC CAGGCCTCCT CATTCCAAGA ATCATTGAGA 
GAAGAAAATG AGAAAAGAAG TCACCATAAA GACCACTCAG ACAGTGAATC TACATCATCA 
GATAATTCTG GGAAAAGAAG AAAAGGACCC TTTAAAACCA TTAAGTTTGG GACCAACATT 
GACCTGTCTG ATGACAAAAA GTGGAAGTTA CAGCTACATG AGCTGACTAA ACTTCCTGCC 
TTCGTGAGAG TTGTATCTGC AGGAAATCTT TTAAGCCACG TTGGTCATAC TATACTGGGC 
ATGAACACAG TTCAACTATA CATGAAAGTT CCAGGAAGCA GAACACCAGG TCATCAAGAA 
AATAACAACT TCTGTTCAGT TAATATAAAT ATTGGCCCAG GTGACTGTGA ATGGTTTGTT 
GTTCCTGAAG GCTACTGGGG TGTTTTGAAT GACTTCTGTG AAAAAAATAA TTTGAATTTC 
TTAATGGGTT CTTGGTGGCC CAACCTTGAA GATCTATATG AAGCAAATGT TCCAGTGTAT 
AGGTTTATTC AGCGACCTGG AGATCTGGTC TGGATAAATG CTGGCACTGT TCATTGGGTT 
CAAGCTATTG GCTGGTGCAA CAATATTGCT TGGAATGTTG GTCCACTTAC AGCCTGTCAG 
TATAAGTTAG CAGTGGAACG TTATGAATGG AACAAGTTGC AAAATGTAAA GTCAATAGTA 
CCCATGGTTC ATCTTTCCTG GAATATGGCA CGAAATATCA AGGTTTCAGA TCCAAAGCTT 
TTTGAAATGA TTAAGTATTG TCTTCTGAGA ACGCTGAAGC AATGTCAGAC ATTGAGGGAA 
GCTCTAATTG CTGCAGGAAA AGAGATCATA TGGCACGGGC GGACAAAAGA AGAACCAGCT 
CATTATTGTA GTATTTGTGA GGTGGAGGTT TTTGATCTGC TTTTTGTCAC TAATGAGAGT 
AATTCTCGAA AAACCTACAT AGTACATTGC CAAGATTGTG CACGAAAAAC AAGTGGGAAT 
CTGGAAAATT TTGTGGTGCT AGAACAGTAC AAAATGGAGG ATCTGATGCA AGTCTATGAC 
CAATTTACAT TAGCTCCTCC ATTACCATCC GCCTCATCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kdm6a ( XM_006527596.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527659.1 Mus musculus lysine (K)-specific demethylase 6A (Kdm6a), transcript variant X4, mRNA.

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CloneID	OMu55893
Clone ID Related Accession (Same CDS sequence)	XM_006527596.3 , XM_006527596.4
Accession Version	XM_006527596.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	lysine-specific demethylase 6A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006527596.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCCG CCGCCGCCGC CGCCGCTTTC 
GGTGATGAGG AAAAGAAAAT GGCGGCGGGA AAAGCGAGCG GCGAGAGCGA GGAGGCGTCC 
CCCAGCCTGA CAGCGGAGGA GAGGGAGGCG CTCGGCGGAC TGGACAGCCG CCTTTTCGGG 
TTCGTGAGGT TTCATGAAGA TGGCGCCAGG ATGAAGGCCC TGCTGGGCAA GGCTGTTCGC 
TGCTACGAAT CTCTAATCTT AAAAGCTGAA GGGAAAGTGG AGTCTGATTT CTTTTGTCAA 
TTAGGTCACT TCAACCTCTT ATTGGAAGAT TATCCAAAAG CATTATCTGC ATACCAGAGG 
TACTACAGTT TACAGTCTGA TTACTGGAAG AATGCTGCCT TTTTATATGG TCTTGGTTTG 
GTCTACTTCC ATTACAATGC ATTTCAGTGG GCTATTAAAG CATTTCAGGA GGTGCTTTAT 
GTCGATCCCA GCTTTTGTCG AGCCAAGGAA ATTCATTTAC GACTTGGGCT TATGTTCAAA 
GTGAACACAG ACTATGAGTC TAGTTTAAAG CATTTTCAGT TAGCTTTGGT TGACTGTAAT 
CCCTGCACTT TGTCCAATGC TGAAATTCAG TTTCACATTG CCCACTTATA TGAAACCCAG 
AGGAAGTATC ATTCTGCAAA AGAAGCTTAT GAGCAACTTT TGCAGACAGA AAACCTTTCT 
GCACAAGTAA AAGCAACTAT TTTACAACAA TTAGGTTGGA TGCATCACAC TGTGGATCTC 
CTGGGAGATA AGGCCACCAA GGAAAGTTAT GCTATTCAGT ATCTCCAGAA GTCCTTGGAA 
GCAGATCCAA ATTCTGGCCA GTCCTGGTAT TTCCTTGGAA GGTGCTATTC AAGTATTGGG 
AAAGTTCAGG ATGCCTTTAT ATCTTACAGG CAATCTATTG ATAAATCAGA AGCAAGTGCA 
GATACATGGT GTTCAATAGG TGTGCTCTAT CAACAGCAAA ATCAGCCTAT GGATGCTTTG 
CAAGCTTATA TTTGTGCTGT ACAATTGGAC CACGGTCATG CTGCAGCCTG GATGGATCTA 
GGCACTCTCT ATGAATCCTG CAACCAACCT CAGGATGCTA TTAAATGCTA TTTAAATGCA 
ACTAGAAGCA AAAATTGTAG TAATACCTCT GGACTTGCAG CACGAATTAA GTATTTACAG 
GCATGTAAAC CTCATCATCC AAATACTGAA CCTGTATTAG GCCTCAGTCA AACACCAATT 
TCACAGCAGT CCTTGCCACT ACACATGATT CCTCCTAGCC AAGTAGATGA CCTGTCCAGT 
CCTGCCAAGA GGAAAAGAAC ATCTAGTCCA ACAAAGAATA CTTCTGATAA TTGGAGTGGT 
GGCAATGCAC CACCTCCAGT AGAACAACAA ACTCATTCAT GGTGTTTGAC ACCACAGAAA 
TTACAGCACT TGGAACAGCT CCGAGCAAAC AGAAATAATT TAAATCCAGC ACAGAAACTA 
ATGCTGGAAC AGCTGGAAAG TCAGTTTGTC TTAATGCAGC AACACCAAAT GAGACAAACA 
GGAGTTGCAC AGGTACGGCC TACTGGAATT CTTAATGGGC CAACAGTTGA CTCATCACTG 
CCTACAAACT CAGTTTCTGG CCAGCAGCCA CAGCTTCCTC TGACCAGAAT GCCTAGTGTC 
TCTCAGCCTG GAGTCCACAC TGCCTGTCCT AGGCAGACTT TGGCCAATGG ACCCTTTTCT 
GCAGGCCATG TTCCCTGTAG CACATCAAGA ACACTGGGAA GTACAGACAC TGTTTTGATA 
GGCAATAATC ATGTAACAGG AAGTGGAAGT AATGGAAACG TGCCTTACCT GCAGCGAAAC 
GCACCCACTC TACCTCATAA CCGCACAAAC CTGACCAGCA GCACAGAGGA GCCGTGGAAA 
AACCAACTAT CTAACTCCAC TCAGGGGCTT CACAAAGGTC CGAGTTCACA TTTGGCAGGT 
CCTAATGGTG AACGACCTCT ATCTTCCACT GGGCCTTCCC AGCATCTCCA GGCAGCTGGC 
TCTGGTATTC AGAATCAGAA TGGACATCCC ACCCTGCCTA GCAATTCAGT AACACAGGGG 
GCTGCTCTCA ATCACCTCTC CTCTCACACT GCTACCTCAG GTGGACAACA AGGCATTACC 
TTAACCAAAG AGAGCAAGCC TTCAGGAAAC ACATTGACGG TGCCTGAAAC AAGCAGGCAA 
ACTGGAGAGA CACCTAACAG CACTGCCAGT GTTGAGGGAC TTCCTAATCA TGTCCATCAG 
GTGATGGCAG ATGCTGTTTG CAGTCCTAGC CATGGAGATT CTAAGTCACC AGGTTTACTA 
AGTTCAGACA ATCCTCAGCT CTCTGCCTTG TTGATGGGAA AAGCTAATAA CAATGTGGGT 
CCTGGAACCT GTGACAAAGT CAATAACATC CACCCAACTG TCCATACAAA GACTGATAAT 
TCTGTTGCCT CTTCACCATC TTCAGCCATT TCCACAGCAA CACCTTCTCC TAAGTCCACT 
GAACAGACAA CCACAAACAG TGTTACCAGC CTTAACAGCC CTCACAGTGG GCTGCACACA 
ATTAATGGAG AAGGAATGGA AGAATCTCAG AGCCCCATTA AAACAGATCT GCTTCTAGTT 
AGCCACAGAC CTAGTCCTCA GATCATACCA TCAATGTCTG TGTCCATATA TCCCAGCTCA 
GCAGAAGTTC TGAAAGCTTG CAGGAATCTA GGTAAAAACG GCCTGTCTAA TAGTAGCATT 
CTGTTGGATA AATGTCCGCC TCCAAGACCA CCATCCTCAC CATACCCTCC CTTGCCAAAG 
GACAAGTTGA ATCCACCTAC ACCTAGTATT TATTTGGAAA ATAAACGTGA TGCTTTCTTT 
CCTCCATTAC ATCAATTTTG TACAAACCCA AACAACCCTG TTACAGTAAT ACGTGGCCTT 
GCTGGAGCTC TTAAATTAGA CTTGGGACTT TTCTCTACTA AAACTTTGGT GGAAGCTAAC 
AATGAACATA TGGTAGAAGT GAGGACACAG TTGTTACAAC CAGCAGATGA AAATTGGGAC 
CCTACTGGAA CCAAGAAAAT CTGGCACTGT GAAAGTAATA GATCTCATAC TACAATTGCT 
AAATATGCTC AGTACCAGGC CTCCTCATTC CAAGAATCAT TGAGAGAAGA AAATGAGAAA 
AGAAGTCACC ATAAAGACCA CTCAGACAGT GAATCTACAT CATCAGATAA TTCTGGGAAA 
AGAAGAAAAG GACCCTTTAA AACCATTAAG TTTGGGACCA ACATTGACCT GTCTGATGAC 
AAAAAGTGGA AGTTACAGCT ACATGAGCTG ACTAAACTTC CTGCCTTCGT GAGAGTTGTA 
TCTGCAGGAA ATCTTTTAAG CCACGTTGGT CATACTATAC TGGGCATGAA CACAGTTCAA 
CTATACATGA AAGTTCCAGG AAGCAGAACA CCAGGTCATC AAGAAAATAA CAACTTCTGT 
TCAGTTAATA TAAATATTGG CCCAGGTGAC TGTGAATGGT TTGTTGTTCC TGAAGGCTAC 
TGGGGTGTTT TGAATGACTT CTGTGAAAAA AATAATTTGA ATTTCTTAAT GGGTTCTTGG 
TGGCCCAACC TTGAAGATCT ATATGAAGCA AATGTTCCAG TGTATAGGTT TATTCAGCGA 
CCTGGAGATC TGGTCTGGAT AAATGCTGGC ACTGTTCATT GGGTTCAAGC TATTGGCTGG 
TGCAACAATA TTGCTTGGAA TGTTGGTCCA CTTACAGCCT GTCAGTATAA GTTAGCAGTG 
GAACGTTATG AATGGAACAA GTTGCAAAAT GTAAAGTCAA TAGTACCCAT GGTTCATCTT 
TCCTGGAATA TGGCACGAAA TATCAAGGTT TCAGATCCAA AGCTTTTTGA AATGATTAAG 
TATTGTCTTC TGAGAACGCT GAAGCAATGT CAGACATTGA GGGAAGCTCT AATTGCTGCA 
GGAAAAGAGA TCATATGGCA CGGGCGGACA AAAGAAGAAC CAGCTCATTA TTGTAGTATT 
TGTGAGGTGG AGGTTTTTGA TCTGCTTTTT GTCACTAATG AGAGTAATTC TCGAAAAACC 
TACATAGTAC ATTGCCAAGA TTGTGCACGA AAAACAAGTG GGAATCTGGA AAATTTTGTG 
GTGCTAGAAC AGTACAAAAT GGAGGATCTG ATGCAAGTCT ATGACCAATT TACATTAGTA 
AGTGAAATCA ACATGCTCCT CCATTACCAT CCGCCTCATC TTGATATTGT TCCATGGACA 
TTAAACATGA GACCTTTTCT GCTATTCAGA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006527659.1
CDS	2145..6440
Translation	MKSCGVSLATAAAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARMKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATILQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKNCSNTSGLAARIKYLQACKPHHPNTEPVLGLSQTPISQQSLPLHMIPPSQVDDLSSPAKRKRTSSPTKNTSDNWSGGNAPPPVEQQTHSWCLTPQKLQHLEQLRANRNNLNPAQKLMLEQLESQFVLMQQHQMRQTGVAQVRPTGILNGPTVDSSLPTNSVSGQQPQLPLTRMPSVSQPGVHTACPRQTLANGPFSAGHVPCSTSRTLGSTDTVLIGNNHVTGSGSNGNVPYLQRNAPTLPHNRTNLTSSTEEPWKNQLSNSTQGLHKGPSSHLAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNTLTVPETSRQTGETPNSTASVEGLPNHVHQVMADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGPGTCDKVNNIHPTVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPIKTDLLLVSHRPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGKRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQNVKSIVPMVHLSWNMARNIKVSDPKLFEMIKYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLVSEINMLLHYHPPHLDIVPWTLNMRPFLLFRK

Target ORF information:

RefSeq Version	XM_006527596.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus lysine (K)-specific demethylase 6A (Kdm6a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527596.4

ORF Insert Sequence:

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCCG CCGCCGCCGC CGCCGCTTTC 
GGTGATGAGG AAAAGAAAAT GGCGGCGGGA AAAGCGAGCG GCGAGAGCGA GGAGGCGTCC 
CCCAGCCTGA CAGCGGAGGA GAGGGAGGCG CTCGGCGGAC TGGACAGCCG CCTTTTCGGG 
TTCGTGAGGT TTCATGAAGA TGGCGCCAGG ATGAAGGCCC TGCTGGGCAA GGCTGTTCGC 
TGCTACGAAT CTCTAATCTT AAAAGCTGAA GGGAAAGTGG AGTCTGATTT CTTTTGTCAA 
TTAGGTCACT TCAACCTCTT ATTGGAAGAT TATCCAAAAG CATTATCTGC ATACCAGAGG 
TACTACAGTT TACAGTCTGA TTACTGGAAG AATGCTGCCT TTTTATATGG TCTTGGTTTG 
GTCTACTTCC ATTACAATGC ATTTCAGTGG GCTATTAAAG CATTTCAGGA GGTGCTTTAT 
GTCGATCCCA GCTTTTGTCG AGCCAAGGAA ATTCATTTAC GACTTGGGCT TATGTTCAAA 
GTGAACACAG ACTATGAGTC TAGTTTAAAG CATTTTCAGT TAGCTTTGGT TGACTGTAAT 
CCCTGCACTT TGTCCAATGC TGAAATTCAG TTTCACATTG CCCACTTATA TGAAACCCAG 
AGGAAGTATC ATTCTGCAAA AGAAGCTTAT GAGCAACTTT TGCAGACAGA AAACCTTTCT 
GCACAAGTAA AAGCAACTAT TTTACAACAA TTAGGTTGGA TGCATCACAC TGTGGATCTC 
CTGGGAGATA AGGCCACCAA GGAAAGTTAT GCTATTCAGT ATCTCCAGAA GTCCTTGGAA 
GCAGATCCAA ATTCTGGCCA GTCCTGGTAT TTCCTTGGAA GGTGCTATTC AAGTATTGGG 
AAAGTTCAGG ATGCCTTTAT ATCTTACAGG CAATCTATTG ATAAATCAGA AGCAAGTGCA 
GATACATGGT GTTCAATAGG TGTGCTCTAT CAACAGCAAA ATCAGCCTAT GGATGCTTTG 
CAAGCTTATA TTTGTGCTGT ACAATTGGAC CACGGTCATG CTGCAGCCTG GATGGATCTA 
GGCACTCTCT ATGAATCCTG CAACCAACCT CAGGATGCTA TTAAATGCTA TTTAAATGCA 
ACTAGAAGCA AAAATTGTAG TAATACCTCT GGACTTGCAG CACGAATTAA GTATTTACAG 
GCATGTAAAC CTCATCATCC AAATACTGAA CCTGTATTAG GCCTCAGTCA AACACCAATT 
TCACAGCAGT CCTTGCCACT ACACATGATT CCTCCTAGCC AAGTAGATGA CCTGTCCAGT 
CCTGCCAAGA GGAAAAGAAC ATCTAGTCCA ACAAAGAATA CTTCTGATAA TTGGAGTGGT 
GGCAATGCAC CACCTCCAGT AGAACAACAA ACTCATTCAT GGTGTTTGAC ACCACAGAAA 
TTACAGCACT TGGAACAGCT CCGAGCAAAC AGAAATAATT TAAATCCAGC ACAGAAACTA 
ATGCTGGAAC AGCTGGAAAG TCAGTTTGTC TTAATGCAGC AACACCAAAT GAGACAAACA 
GGAGTTGCAC AGGTACGGCC TACTGGAATT CTTAATGGGC CAACAGTTGA CTCATCACTG 
CCTACAAACT CAGTTTCTGG CCAGCAGCCA CAGCTTCCTC TGACCAGAAT GCCTAGTGTC 
TCTCAGCCTG GAGTCCACAC TGCCTGTCCT AGGCAGACTT TGGCCAATGG ACCCTTTTCT 
GCAGGCCATG TTCCCTGTAG CACATCAAGA ACACTGGGAA GTACAGACAC TGTTTTGATA 
GGCAATAATC ATGTAACAGG AAGTGGAAGT AATGGAAACG TGCCTTACCT GCAGCGAAAC 
GCACCCACTC TACCTCATAA CCGCACAAAC CTGACCAGCA GCACAGAGGA GCCGTGGAAA 
AACCAACTAT CTAACTCCAC TCAGGGGCTT CACAAAGGTC CGAGTTCACA TTTGGCAGGT 
CCTAATGGTG AACGACCTCT ATCTTCCACT GGGCCTTCCC AGCATCTCCA GGCAGCTGGC 
TCTGGTATTC AGAATCAGAA TGGACATCCC ACCCTGCCTA GCAATTCAGT AACACAGGGG 
GCTGCTCTCA ATCACCTCTC CTCTCACACT GCTACCTCAG GTGGACAACA AGGCATTACC 
TTAACCAAAG AGAGCAAGCC TTCAGGAAAC ACATTGACGG TGCCTGAAAC AAGCAGGCAA 
ACTGGAGAGA CACCTAACAG CACTGCCAGT GTTGAGGGAC TTCCTAATCA TGTCCATCAG 
GTGATGGCAG ATGCTGTTTG CAGTCCTAGC CATGGAGATT CTAAGTCACC AGGTTTACTA 
AGTTCAGACA ATCCTCAGCT CTCTGCCTTG TTGATGGGAA AAGCTAATAA CAATGTGGGT 
CCTGGAACCT GTGACAAAGT CAATAACATC CACCCAACTG TCCATACAAA GACTGATAAT 
TCTGTTGCCT CTTCACCATC TTCAGCCATT TCCACAGCAA CACCTTCTCC TAAGTCCACT 
GAACAGACAA CCACAAACAG TGTTACCAGC CTTAACAGCC CTCACAGTGG GCTGCACACA 
ATTAATGGAG AAGGAATGGA AGAATCTCAG AGCCCCATTA AAACAGATCT GCTTCTAGTT 
AGCCACAGAC CTAGTCCTCA GATCATACCA TCAATGTCTG TGTCCATATA TCCCAGCTCA 
GCAGAAGTTC TGAAAGCTTG CAGGAATCTA GGTAAAAACG GCCTGTCTAA TAGTAGCATT 
CTGTTGGATA AATGTCCGCC TCCAAGACCA CCATCCTCAC CATACCCTCC CTTGCCAAAG 
GACAAGTTGA ATCCACCTAC ACCTAGTATT TATTTGGAAA ATAAACGTGA TGCTTTCTTT 
CCTCCATTAC ATCAATTTTG TACAAACCCA AACAACCCTG TTACAGTAAT ACGTGGCCTT 
GCTGGAGCTC TTAAATTAGA CTTGGGACTT TTCTCTACTA AAACTTTGGT GGAAGCTAAC 
AATGAACATA TGGTAGAAGT GAGGACACAG TTGTTACAAC CAGCAGATGA AAATTGGGAC 
CCTACTGGAA CCAAGAAAAT CTGGCACTGT GAAAGTAATA GATCTCATAC TACAATTGCT 
AAATATGCTC AGTACCAGGC CTCCTCATTC CAAGAATCAT TGAGAGAAGA AAATGAGAAA 
AGAAGTCACC ATAAAGACCA CTCAGACAGT GAATCTACAT CATCAGATAA TTCTGGGAAA 
AGAAGAAAAG GACCCTTTAA AACCATTAAG TTTGGGACCA ACATTGACCT GTCTGATGAC 
AAAAAGTGGA AGTTACAGCT ACATGAGCTG ACTAAACTTC CTGCCTTCGT GAGAGTTGTA 
TCTGCAGGAA ATCTTTTAAG CCACGTTGGT CATACTATAC TGGGCATGAA CACAGTTCAA 
CTATACATGA AAGTTCCAGG AAGCAGAACA CCAGGTCATC AAGAAAATAA CAACTTCTGT 
TCAGTTAATA TAAATATTGG CCCAGGTGAC TGTGAATGGT TTGTTGTTCC TGAAGGCTAC 
TGGGGTGTTT TGAATGACTT CTGTGAAAAA AATAATTTGA ATTTCTTAAT GGGTTCTTGG 
TGGCCCAACC TTGAAGATCT ATATGAAGCA AATGTTCCAG TGTATAGGTT TATTCAGCGA 
CCTGGAGATC TGGTCTGGAT AAATGCTGGC ACTGTTCATT GGGTTCAAGC TATTGGCTGG 
TGCAACAATA TTGCTTGGAA TGTTGGTCCA CTTACAGCCT GTCAGTATAA GTTAGCAGTG 
GAACGTTATG AATGGAACAA GTTGCAAAAT GTAAAGTCAA TAGTACCCAT GGTTCATCTT 
TCCTGGAATA TGGCACGAAA TATCAAGGTT TCAGATCCAA AGCTTTTTGA AATGATTAAG 
TATTGTCTTC TGAGAACGCT GAAGCAATGT CAGACATTGA GGGAAGCTCT AATTGCTGCA 
GGAAAAGAGA TCATATGGCA CGGGCGGACA AAAGAAGAAC CAGCTCATTA TTGTAGTATT 
TGTGAGGTGG AGGTTTTTGA TCTGCTTTTT GTCACTAATG AGAGTAATTC TCGAAAAACC 
TACATAGTAC ATTGCCAAGA TTGTGCACGA AAAACAAGTG GGAATCTGGA AAATTTTGTG 
GTGCTAGAAC AGTACAAAAT GGAGGATCTG ATGCAAGTCT ATGACCAATT TACATTAGTA 
AGTGAAATCA ACATGCTCCT CCATTACCAT CCGCCTCATC TTGATATTGT TCCATGGACA 
TTAAACATGA GACCTTTTCT GCTATTCAGA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kdm6a ( XM_006527597.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527660.1 Mus musculus lysine (K)-specific demethylase 6A (Kdm6a), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu55894
Clone ID Related Accession (Same CDS sequence)	XM_006527597.4 , XM_006527597.3
Accession Version	XM_006527597.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	lysine-specific demethylase 6A isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006527597.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCCG CCGCCGCCGC CGCCGCTTTC 
GGTGATGAGG AAAAGAAAAT GGCGGCGGGA AAAGCGAGCG GCGAGAGCGA GGAGGCGTCC 
CCCAGCCTGA CAGCGGAGGA GAGGGAGGCG CTCGGCGGAC TGGACAGCCG CCTTTTCGGG 
TTCGTGAGGT TTCATGAAGA TGGCGCCAGG ATGAAGGCCC TGCTGGGCAA GGCTGTTCGC 
TGCTACGAAT CTCTAATCTT AAAAGCTGAA GGGAAAGTGG AGTCTGATTT CTTTTGTCAA 
TTAGGTCACT TCAACCTCTT ATTGGAAGAT TATCCAAAAG CATTATCTGC ATACCAGAGG 
TACTACAGTT TACAGTCTGA TTACTGGAAG AATGCTGCCT TTTTATATGG TCTTGGTTTG 
GTCTACTTCC ATTACAATGC ATTTCAGTGG GCTATTAAAG CATTTCAGGA GGTGCTTTAT 
GTCGATCCCA GCTTTTGTCG AGCCAAGGAA ATTCATTTAC GACTTGGGCT TATGTTCAAA 
GTGAACACAG ACTATGAGTC TAGTTTAAAG CATTTTCAGT TAGCTTTGGT TGACTGTAAT 
CCCTGCACTT TGTCCAATGC TGAAATTCAG TTTCACATTG CCCACTTATA TGAAACCCAG 
AGGAAGTATC ATTCTGCAAA AGAAGCTTAT GAGCAACTTT TGCAGACAGA AAACCTTTCT 
GCACAAGTAA AAGCAACTAT TTTACAACAA TTAGGTTGGA TGCATCACAC TGTGGATCTC 
CTGGGAGATA AGGCCACCAA GGAAAGTTAT GCTATTCAGT ATCTCCAGAA GTCCTTGGAA 
GCAGATCCAA ATTCTGGCCA GTCCTGGTAT TTCCTTGGAA GGTGCTATTC AAGTATTGGG 
AAAGTTCAGG ATGCCTTTAT ATCTTACAGG CAATCTATTG ATAAATCAGA AGCAAGTGCA 
GATACATGGT GTTCAATAGG TGTGCTCTAT CAACAGCAAA ATCAGCCTAT GGATGCTTTG 
CAAGCTTATA TTTGTGCTGT ACAATTGGAC CACGGTCATG CTGCAGCCTG GATGGATCTA 
GGCACTCTCT ATGAATCCTG CAACCAACCT CAGGATGCTA TTAAATGCTA TTTAAATGCA 
ACTAGAAGCA AAAATTGTAG TAATACCTCT GGACTTGCAG CACGAATTAA GTATTTACAG 
GCATGTAAAC CTCATCATCC AAATACTGAA CCTGTATTAG GCCTCAGTCA AACACCAATT 
TCACAGCAGT CCTTGCCACT ACACATGATT CCTCCTAGCC AAGTAGATGA CCTGTCCAGT 
CCTGCCAAGA GGAAAAGAAC ATCTAGTCCA ACAAAGAATA CTTCTGATAA TTGGAGTGGT 
GGCAATGCAC CACCTCCAGT AGAACAACAA ACTCATTCAT GGTGTTTGAC ACCACAGAAA 
TTACAGCACT TGGAACAGCT CCGAGCAAAC AGAAATAATT TAAATCCAGC ACAGAAACTA 
ATGCTGGAAC AGCTGGAAAG TCAGTTTGTC TTAATGCAGC AACACCAAAT GAGACAAACA 
GGAGTTGCAC AGGTACGGCC TACTGGAATT CTTAATGGGC CAACAGTTGA CTCATCACTG 
CCTACAAACT CAGTTTCTGG CCAGCAGCCA CAGCTTCCTC TGACCAGAAT GCCTAGTGTC 
TCTCAGCCTG GAGTCCACAC TGCCTGTCCT AGGCAGACTT TGGCCAATGG ACCCTTTTCT 
GCAGGCCATG TTCCCTGTAG CACATCAAGA ACACTGGGAA GTACAGACAC TGTTTTGATA 
GGCAATAATC ATGTAACAGG AAGTGGAAGT AATGGAAACG TGCCTTACCT GCAGCGAAAC 
GCACCCACTC TACCTCATAA CCGCACAAAC CTGACCAGCA GCACAGAGGA GCCGTGGAAA 
AACCAACTAT CTAACTCCAC TCAGGGGCTT CACAAAGGTC CGAGTTCACA TTTGGCAGGT 
CCTAATGGTG AACGACCTCT ATCTTCCACT GGGCCTTCCC AGCATCTCCA GGCAGCTGGC 
TCTGGTATTC AGAATCAGAA TGGACATCCC ACCCTGCCTA GCAATTCAGT AACACAGGGG 
GCTGCTCTCA ATCACCTCTC CTCTCACACT GCTACCTCAG GTGGACAACA AGGCATTACC 
TTAACCAAAG AGAGCAAGCC TTCAGGAAAC ACATTGACGG TGCCTGAAAC AAGCAGGCAA 
ACTGGAGAGA CACCTAACAG CACTGCCAGT GTTGAGGGAC TTCCTAATCA TGTCCATCAG 
GTGATGGCAG ATGCTGTTTG CAGTCCTAGC CATGGAGATT CTAAGTCACC AGGTTTACTA 
AGTTCAGACA ATCCTCAGCT CTCTGCCTTG TTGATGGGAA AAGCTAATAA CAATGTGGGT 
CCTGGAACCT GTGACAAAGT CAATAACATC CACCCAACTG TCCATACAAA GACTGATAAT 
TCTGTTGCCT CTTCACCATC TTCAGCCATT TCCACAGCAA CACCTTCTCC TAAGTCCACT 
GAACAGACAA CCACAAACAG TGTTACCAGC CTTAACAGCC CTCACAGTGG GCTGCACACA 
ATTAATGGAG AAGGAATGGA AGAATCTCAG AGCCCCATTA AAACAGATCT GCTTCTAGTT 
AGCCACAGAC CTAGTCCTCA GATCATACCA TCAATGTCTG TGTCCATATA TCCCAGCTCA 
GCAGAAGTTC TGAAAGCTTG CAGGAATCTA GGTAAAAACG GCCTGTCTAA TAGTAGCATT 
CTGTTGGATA AATGTCCGCC TCCAAGACCA CCATCCTCAC CATACCCTCC CTTGCCAAAG 
GACAAGTTGA ATCCACCTAC ACCTAGTATT TATTTGGAAA ATAAACGTGA TGCTTTCTTT 
CCTCCATTAC ATCAATTTTG TACAAACCCA AACAACCCTG TTACAGTAAT ACGTGGCCTT 
GCTGGAGCTC TTAAATTAGA CTTGGGACTT TTCTCTACTA AAACTTTGGT GGAAGCTAAC 
AATGAACATA TGGTAGAAGT GAGGACACAG TTGTTACAAC CAGCAGATGA AAATTGGGAC 
CCTACTGGAA CCAAGAAAAT CTGGCACTGT GAAAGTAATA GATCTCATAC TACAATTGCT 
AAATATGCTC AGTACCAGGC CTCCTCATTC CAAGAATCAT TGAGAGAAGA AAATGAGAAA 
AGAAGTCACC ATAAAGACCA CTCAGACAGT GAATCTACAT CATCAGATAA TTCTGGGAAA 
AGAAGAAAAG GACCCTTTAA AACCATTAAG TTTGGGACCA ACATTGACCT GTCTGATGAC 
AAAAAGTGGA AGTTACAGCT ACATGAGCTG ACTAAACTTC CTGCCTTCGT GAGAGTTGTA 
TCTGCAGGAA ATCTTTTAAG CCACGTTGGT CATACTATAC TGGGCATGAA CACAGTTCAA 
CTATACATGA AAGTTCCAGG AAGCAGAACA CCAGGTCATC AAGAAAATAA CAACTTCTGT 
TCAGTTAATA TAAATATTGG CCCAGGTGAC TGTGAATGGT TTGTTGTTCC TGAAGGCTAC 
TGGGGTGTTT TGAATGACTT CTGTGAAAAA AATAATTTGA ATTTCTTAAT GGGTTCTTGG 
TGGCCCAACC TTGAAGATCT ATATGAAGCA AATGTTCCAG TGTATAGGTT TATTCAGCGA 
CCTGGAGATC TGGTCTGGAT AAATGCTGGC ACTGTTCATT GGGTTCAAGC TATTGGCTGG 
TGCAACAATA TTGCTTGGAA TGTTGGTCCA CTTACAGCCT GTCAGTATAA GTTAGCAGTG 
GAACGTTATG AATGGAACAA GTTGCAAAAT GTAAAGTCAA TAGTACCCAT GGTTCATCTT 
TCCTGGAATA TGGCACGAAA TATCAAGGTT TCAGATCCAA AGCTTTTTGA AATGATTAAG 
TATTGTCTTC TGAGAACGCT GAAGCAATGT CAGACATTGA GGGAAGCTCT AATTGCTGCA 
GGAAAAGAGA TCATATGGCA CGGGCGGACA AAAGAAGAAC CAGCTCATTA TTGTAGTATT 
TGTGAGGTGG AGGTTTTTGA TCTGCTTTTT GTCACTAATG AGAGTAATTC TCGAAAAACC 
TACATAGTAC ATTGCCAAGA TTGTGCACGA AAAACAAGTG GGAATCTGGA AAATTTTGTG 
GTGCTAGAAC AGTACAAAAT GGAGGATCTG ATGCAAGTCT ATGACCAATT TACATTAGCT 
CCTCCATTAC CATCCGCCTC ATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006527660.1
CDS	2145..6371
Translation	MKSCGVSLATAAAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARMKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATILQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKNCSNTSGLAARIKYLQACKPHHPNTEPVLGLSQTPISQQSLPLHMIPPSQVDDLSSPAKRKRTSSPTKNTSDNWSGGNAPPPVEQQTHSWCLTPQKLQHLEQLRANRNNLNPAQKLMLEQLESQFVLMQQHQMRQTGVAQVRPTGILNGPTVDSSLPTNSVSGQQPQLPLTRMPSVSQPGVHTACPRQTLANGPFSAGHVPCSTSRTLGSTDTVLIGNNHVTGSGSNGNVPYLQRNAPTLPHNRTNLTSSTEEPWKNQLSNSTQGLHKGPSSHLAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNTLTVPETSRQTGETPNSTASVEGLPNHVHQVMADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGPGTCDKVNNIHPTVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPIKTDLLLVSHRPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGKRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQNVKSIVPMVHLSWNMARNIKVSDPKLFEMIKYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLAPPLPSASS

Target ORF information:

RefSeq Version	XM_006527597.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus lysine (K)-specific demethylase 6A (Kdm6a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527597.4

ORF Insert Sequence:

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCCG CCGCCGCCGC CGCCGCTTTC 
GGTGATGAGG AAAAGAAAAT GGCGGCGGGA AAAGCGAGCG GCGAGAGCGA GGAGGCGTCC 
CCCAGCCTGA CAGCGGAGGA GAGGGAGGCG CTCGGCGGAC TGGACAGCCG CCTTTTCGGG 
TTCGTGAGGT TTCATGAAGA TGGCGCCAGG ATGAAGGCCC TGCTGGGCAA GGCTGTTCGC 
TGCTACGAAT CTCTAATCTT AAAAGCTGAA GGGAAAGTGG AGTCTGATTT CTTTTGTCAA 
TTAGGTCACT TCAACCTCTT ATTGGAAGAT TATCCAAAAG CATTATCTGC ATACCAGAGG 
TACTACAGTT TACAGTCTGA TTACTGGAAG AATGCTGCCT TTTTATATGG TCTTGGTTTG 
GTCTACTTCC ATTACAATGC ATTTCAGTGG GCTATTAAAG CATTTCAGGA GGTGCTTTAT 
GTCGATCCCA GCTTTTGTCG AGCCAAGGAA ATTCATTTAC GACTTGGGCT TATGTTCAAA 
GTGAACACAG ACTATGAGTC TAGTTTAAAG CATTTTCAGT TAGCTTTGGT TGACTGTAAT 
CCCTGCACTT TGTCCAATGC TGAAATTCAG TTTCACATTG CCCACTTATA TGAAACCCAG 
AGGAAGTATC ATTCTGCAAA AGAAGCTTAT GAGCAACTTT TGCAGACAGA AAACCTTTCT 
GCACAAGTAA AAGCAACTAT TTTACAACAA TTAGGTTGGA TGCATCACAC TGTGGATCTC 
CTGGGAGATA AGGCCACCAA GGAAAGTTAT GCTATTCAGT ATCTCCAGAA GTCCTTGGAA 
GCAGATCCAA ATTCTGGCCA GTCCTGGTAT TTCCTTGGAA GGTGCTATTC AAGTATTGGG 
AAAGTTCAGG ATGCCTTTAT ATCTTACAGG CAATCTATTG ATAAATCAGA AGCAAGTGCA 
GATACATGGT GTTCAATAGG TGTGCTCTAT CAACAGCAAA ATCAGCCTAT GGATGCTTTG 
CAAGCTTATA TTTGTGCTGT ACAATTGGAC CACGGTCATG CTGCAGCCTG GATGGATCTA 
GGCACTCTCT ATGAATCCTG CAACCAACCT CAGGATGCTA TTAAATGCTA TTTAAATGCA 
ACTAGAAGCA AAAATTGTAG TAATACCTCT GGACTTGCAG CACGAATTAA GTATTTACAG 
GCATGTAAAC CTCATCATCC AAATACTGAA CCTGTATTAG GCCTCAGTCA AACACCAATT 
TCACAGCAGT CCTTGCCACT ACACATGATT CCTCCTAGCC AAGTAGATGA CCTGTCCAGT 
CCTGCCAAGA GGAAAAGAAC ATCTAGTCCA ACAAAGAATA CTTCTGATAA TTGGAGTGGT 
GGCAATGCAC CACCTCCAGT AGAACAACAA ACTCATTCAT GGTGTTTGAC ACCACAGAAA 
TTACAGCACT TGGAACAGCT CCGAGCAAAC AGAAATAATT TAAATCCAGC ACAGAAACTA 
ATGCTGGAAC AGCTGGAAAG TCAGTTTGTC TTAATGCAGC AACACCAAAT GAGACAAACA 
GGAGTTGCAC AGGTACGGCC TACTGGAATT CTTAATGGGC CAACAGTTGA CTCATCACTG 
CCTACAAACT CAGTTTCTGG CCAGCAGCCA CAGCTTCCTC TGACCAGAAT GCCTAGTGTC 
TCTCAGCCTG GAGTCCACAC TGCCTGTCCT AGGCAGACTT TGGCCAATGG ACCCTTTTCT 
GCAGGCCATG TTCCCTGTAG CACATCAAGA ACACTGGGAA GTACAGACAC TGTTTTGATA 
GGCAATAATC ATGTAACAGG AAGTGGAAGT AATGGAAACG TGCCTTACCT GCAGCGAAAC 
GCACCCACTC TACCTCATAA CCGCACAAAC CTGACCAGCA GCACAGAGGA GCCGTGGAAA 
AACCAACTAT CTAACTCCAC TCAGGGGCTT CACAAAGGTC CGAGTTCACA TTTGGCAGGT 
CCTAATGGTG AACGACCTCT ATCTTCCACT GGGCCTTCCC AGCATCTCCA GGCAGCTGGC 
TCTGGTATTC AGAATCAGAA TGGACATCCC ACCCTGCCTA GCAATTCAGT AACACAGGGG 
GCTGCTCTCA ATCACCTCTC CTCTCACACT GCTACCTCAG GTGGACAACA AGGCATTACC 
TTAACCAAAG AGAGCAAGCC TTCAGGAAAC ACATTGACGG TGCCTGAAAC AAGCAGGCAA 
ACTGGAGAGA CACCTAACAG CACTGCCAGT GTTGAGGGAC TTCCTAATCA TGTCCATCAG 
GTGATGGCAG ATGCTGTTTG CAGTCCTAGC CATGGAGATT CTAAGTCACC AGGTTTACTA 
AGTTCAGACA ATCCTCAGCT CTCTGCCTTG TTGATGGGAA AAGCTAATAA CAATGTGGGT 
CCTGGAACCT GTGACAAAGT CAATAACATC CACCCAACTG TCCATACAAA GACTGATAAT 
TCTGTTGCCT CTTCACCATC TTCAGCCATT TCCACAGCAA CACCTTCTCC TAAGTCCACT 
GAACAGACAA CCACAAACAG TGTTACCAGC CTTAACAGCC CTCACAGTGG GCTGCACACA 
ATTAATGGAG AAGGAATGGA AGAATCTCAG AGCCCCATTA AAACAGATCT GCTTCTAGTT 
AGCCACAGAC CTAGTCCTCA GATCATACCA TCAATGTCTG TGTCCATATA TCCCAGCTCA 
GCAGAAGTTC TGAAAGCTTG CAGGAATCTA GGTAAAAACG GCCTGTCTAA TAGTAGCATT 
CTGTTGGATA AATGTCCGCC TCCAAGACCA CCATCCTCAC CATACCCTCC CTTGCCAAAG 
GACAAGTTGA ATCCACCTAC ACCTAGTATT TATTTGGAAA ATAAACGTGA TGCTTTCTTT 
CCTCCATTAC ATCAATTTTG TACAAACCCA AACAACCCTG TTACAGTAAT ACGTGGCCTT 
GCTGGAGCTC TTAAATTAGA CTTGGGACTT TTCTCTACTA AAACTTTGGT GGAAGCTAAC 
AATGAACATA TGGTAGAAGT GAGGACACAG TTGTTACAAC CAGCAGATGA AAATTGGGAC 
CCTACTGGAA CCAAGAAAAT CTGGCACTGT GAAAGTAATA GATCTCATAC TACAATTGCT 
AAATATGCTC AGTACCAGGC CTCCTCATTC CAAGAATCAT TGAGAGAAGA AAATGAGAAA 
AGAAGTCACC ATAAAGACCA CTCAGACAGT GAATCTACAT CATCAGATAA TTCTGGGAAA 
AGAAGAAAAG GACCCTTTAA AACCATTAAG TTTGGGACCA ACATTGACCT GTCTGATGAC 
AAAAAGTGGA AGTTACAGCT ACATGAGCTG ACTAAACTTC CTGCCTTCGT GAGAGTTGTA 
TCTGCAGGAA ATCTTTTAAG CCACGTTGGT CATACTATAC TGGGCATGAA CACAGTTCAA 
CTATACATGA AAGTTCCAGG AAGCAGAACA CCAGGTCATC AAGAAAATAA CAACTTCTGT 
TCAGTTAATA TAAATATTGG CCCAGGTGAC TGTGAATGGT TTGTTGTTCC TGAAGGCTAC 
TGGGGTGTTT TGAATGACTT CTGTGAAAAA AATAATTTGA ATTTCTTAAT GGGTTCTTGG 
TGGCCCAACC TTGAAGATCT ATATGAAGCA AATGTTCCAG TGTATAGGTT TATTCAGCGA 
CCTGGAGATC TGGTCTGGAT AAATGCTGGC ACTGTTCATT GGGTTCAAGC TATTGGCTGG 
TGCAACAATA TTGCTTGGAA TGTTGGTCCA CTTACAGCCT GTCAGTATAA GTTAGCAGTG 
GAACGTTATG AATGGAACAA GTTGCAAAAT GTAAAGTCAA TAGTACCCAT GGTTCATCTT 
TCCTGGAATA TGGCACGAAA TATCAAGGTT TCAGATCCAA AGCTTTTTGA AATGATTAAG 
TATTGTCTTC TGAGAACGCT GAAGCAATGT CAGACATTGA GGGAAGCTCT AATTGCTGCA 
GGAAAAGAGA TCATATGGCA CGGGCGGACA AAAGAAGAAC CAGCTCATTA TTGTAGTATT 
TGTGAGGTGG AGGTTTTTGA TCTGCTTTTT GTCACTAATG AGAGTAATTC TCGAAAAACC 
TACATAGTAC ATTGCCAAGA TTGTGCACGA AAAACAAGTG GGAATCTGGA AAATTTTGTG 
GTGCTAGAAC AGTACAAAAT GGAGGATCTG ATGCAAGTCT ATGACCAATT TACATTAGCT 
CCTCCATTAC CATCCGCCTC ATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kdm6a ( XM_006527599.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527662.1 Mus musculus lysine (K)-specific demethylase 6A (Kdm6a), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMu55896
Clone ID Related Accession (Same CDS sequence)	XM_006527599.3 , XM_006527599.4
Accession Version	XM_006527599.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4140bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	lysine-specific demethylase 6A isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006527599.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCCG CCGCCGCCGC CGCCGCTTTC 
GGTGATGAGG AAAAGAAAAT GGCGGCGGGA AAAGCGAGCG GCGAGAGCGA GGAGGCGTCC 
CCCAGCCTGA CAGCGGAGGA GAGGGAGGCG CTCGGCGGAC TGGACAGCCG CCTTTTCGGG 
TTCGTGAGGT TTCATGAAGA TGGCGCCAGG ATGAAGGCCC TGCTGGGCAA GGCTGTTCGC 
TGCTACGAAT CTCTAATCTT AAAAGCTGAA GGGAAAGTGG AGTCTGATTT CTTTTGTCAA 
TTAGGTCACT TCAACCTCTT ATTGGAAGAT TATCCAAAAG CATTATCTGC ATACCAGAGG 
TACTACAGTT TACAGTCTGA TTACTGGAAG AATGCTGCCT TTTTATATGG TCTTGGTTTG 
GTCTACTTCC ATTACAATGC ATTTCAGTGG GCTATTAAAG CATTTCAGGA GGTGCTTTAT 
GTCGATCCCA GCTTTTGTCG AGCCAAGGAA ATTCATTTAC GACTTGGGCT TATGTTCAAA 
GTGAACACAG ACTATGAGTC TAGTTTAAAG CATTTTCAGT TAGCTTTGGT TGACTGTAAT 
CCCTGCACTT TGTCCAATGC TGAAATTCAG TTTCACATTG CCCACTTATA TGAAACCCAG 
AGGAAGTATC ATTCTGCAAA AGAAGCTTAT GAGCAACTTT TGCAGACAGA AAACCTTTCT 
GCACAAGTAA AAGCAACTAT TTTACAACAA TTAGGTTGGA TGCATCACAC TGTGGATCTC 
CTGGGAGATA AGGCCACCAA GGAAAGTTAT GCTATTCAGT ATCTCCAGAA GTCCTTGGAA 
GCAGATCCAA ATTCTGGCCA GTCCTGGTAT TTCCTTGGAA GGTGCTATTC AAGTATTGGG 
AAAGTTCAGG ATGCCTTTAT ATCTTACAGG CAATCTATTG ATAAATCAGA AGCAAGTGCA 
GATACATGGT GTTCAATAGG TGTGCTCTAT CAACAGCAAA ATCAGCCTAT GGATGCTTTG 
CAAGCTTATA TTTGTGCTGT ACAATTGGAC CACGGTCATG CTGCAGCCTG GATGGATCTA 
GGCACTCTCT ATGAATCCTG CAACCAACCT CAGGATGCTA TTAAATGCTA TTTAAATGCA 
ACTAGAAGCA AAAATTGTAG TAATACCTCT GGACTTGCAG CACGAATTAA GTATTTACAG 
AATACTTCTG ATAATTGGAG TGGTGGCAAT GCACCACCTC CAGTAGAACA ACAAACTCAT 
TCATGGTGTT TGACACCACA GAAATTACAG CACTTGGAAC AGCTCCGAGC AAACAGAAAT 
AATTTAAATC CAGCACAGAA ACTAATGCTG GAACAGCTGG AAAGTCAGTT TGTCTTAATG 
CAGCAACACC AAATGAGACA AACAGGAGTT GCACAGGTAC GGCCTACTGG AATTCTTAAT 
GGGCCAACAG TTGACTCATC ACTGCCTACA AACTCAGTTT CTGGCCAGCA GCCACAGCTT 
CCTCTGACCA GAATGCCTAG TGTCTCTCAG CCTGGAGTCC ACACTGCCTG TCCTAGGCAG 
ACTTTGGCCA ATGGACCCTT TTCTGCAGGC CATGTTCCCT GTAGCACATC AAGAACACTG 
GGAAGTACAG ACACTGTTTT GATAGGCAAT AATCATGTAA CAGGAAGTGG AAGTAATGGA 
AACGTGCCTT ACCTGCAGCG AAACGCACCC ACTCTACCTC ATAACCGCAC AAACCTGACC 
AGCAGCACAG AGGAGCCGTG GAAAAACCAA CTATCTAACT CCACTCAGGG GCTTCACAAA 
GGTCCGAGTT CACATTTGGC AGGTCCTAAT GGTGAACGAC CTCTATCTTC CACTGGGCCT 
TCCCAGCATC TCCAGGCAGC TGGCTCTGGT ATTCAGAATC AGAATGGACA TCCCACCCTG 
CCTAGCAATT CAGTAACACA GGGGGCTGCT CTCAATCACC TCTCCTCTCA CACTGCTACC 
TCAGGTGGAC AACAAGGCAT TACCTTAACC AAAGAGAGCA AGCCTTCAGG AAACACATTG 
ACGGTGCCTG AAACAAGCAG GCAAACTGGA GAGACACCTA ACAGCACTGC CAGTGTTGAG 
GGACTTCCTA ATCATGTCCA TCAGGTGATG GCAGATGCTG TTTGCAGTCC TAGCCATGGA 
GATTCTAAGT CACCAGGTTT ACTAAGTTCA GACAATCCTC AGCTCTCTGC CTTGTTGATG 
GGAAAAGCTA ATAACAATGT GGGTCCTGGA ACCTGTGACA AAGTCAATAA CATCCACCCA 
ACTGTCCATA CAAAGACTGA TAATTCTGTT GCCTCTTCAC CATCTTCAGC CATTTCCACA 
GCAACACCTT CTCCTAAGTC CACTGAACAG ACAACCACAA ACAGTGTTAC CAGCCTTAAC 
AGCCCTCACA GTGGGCTGCA CACAATTAAT GGAGAAGGAA TGGAAGAATC TCAGAGCCCC 
ATTAAAACAG ATCTGCTTCT AGTTAGCCAC AGACCTAGTC CTCAGATCAT ACCATCAATG 
TCTGTGTCCA TATATCCCAG CTCAGCAGAA GTTCTGAAAG CTTGCAGGAA TCTAGGTAAA 
AACGGCCTGT CTAATAGTAG CATTCTGTTG GATAAATGTC CGCCTCCAAG ACCACCATCC 
TCACCATACC CTCCCTTGCC AAAGGACAAG TTGAATCCAC CTACACCTAG TATTTATTTG 
GAAAATAAAC GTGATGCTTT CTTTCCTCCA TTACATCAAT TTTGTACAAA CCCAAACAAC 
CCTGTTACAG TAATACGTGG CCTTGCTGGA GCTCTTAAAT TAGACTTGGG ACTTTTCTCT 
ACTAAAACTT TGGTGGAAGC TAACAATGAA CATATGGTAG AAGTGAGGAC ACAGTTGTTA 
CAACCAGCAG ATGAAAATTG GGACCCTACT GGAACCAAGA AAATCTGGCA CTGTGAAAGT 
AATAGATCTC ATACTACAAT TGCTAAATAT GCTCAGTACC AGGCCTCCTC ATTCCAAGAA 
TCATTGAGAG AAGAAAATGA GAAAAGAAGT CACCATAAAG ACCACTCAGA CAGTGAATCT 
ACATCATCAG ATAATTCTGG GAAAAGAAGA AAAGGACCCT TTAAAACCAT TAAGTTTGGG 
ACCAACATTG ACCTGTCTGA TGACAAAAAG TGGAAGTTAC AGCTACATGA GCTGACTAAA 
CTTCCTGCCT TCGTGAGAGT TGTATCTGCA GGAAATCTTT TAAGCCACGT TGGTCATACT 
ATACTGGGCA TGAACACAGT TCAACTATAC ATGAAAGTTC CAGGAAGCAG AACACCAGGT 
CATCAAGAAA ATAACAACTT CTGTTCAGTT AATATAAATA TTGGCCCAGG TGACTGTGAA 
TGGTTTGTTG TTCCTGAAGG CTACTGGGGT GTTTTGAATG ACTTCTGTGA AAAAAATAAT 
TTGAATTTCT TAATGGGTTC TTGGTGGCCC AACCTTGAAG ATCTATATGA AGCAAATGTT 
CCAGTGTATA GGTTTATTCA GCGACCTGGA GATCTGGTCT GGATAAATGC TGGCACTGTT 
CATTGGGTTC AAGCTATTGG CTGGTGCAAC AATATTGCTT GGAATGTTGG TCCACTTACA 
GCCTGTCAGT ATAAGTTAGC AGTGGAACGT TATGAATGGA ACAAGTTGCA AAATGTAAAG 
TCAATAGTAC CCATGGTTCA TCTTTCCTGG AATATGGCAC GAAATATCAA GGTTTCAGAT 
CCAAAGCTTT TTGAAATGAT TAAGTATTGT CTTCTGAGAA CGCTGAAGCA ATGTCAGACA 
TTGAGGGAAG CTCTAATTGC TGCAGGAAAA GAGATCATAT GGCACGGGCG GACAAAAGAA 
GAACCAGCTC ATTATTGTAG TATTTGTGAG GTGGAGGTTT TTGATCTGCT TTTTGTCACT 
AATGAGAGTA ATTCTCGAAA AACCTACATA GTACATTGCC AAGATTGTGC ACGAAAAACA 
AGTGGGAATC TGGAAAATTT TGTGGTGCTA GAACAGTACA AAATGGAGGA TCTGATGCAA 
GTCTATGACC AATTTACATT AGTAAGTGAA ATCAACATGC TCCTCCATTA CCATCCGCCT 
CATCTTGATA TTGTTCCATG GACATTAAAC ATGAGACCTT TTCTGCTATT CAGAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006527662.1
CDS	2145..6284
Translation	MKSCGVSLATAAAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARMKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATILQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKNCSNTSGLAARIKYLQNTSDNWSGGNAPPPVEQQTHSWCLTPQKLQHLEQLRANRNNLNPAQKLMLEQLESQFVLMQQHQMRQTGVAQVRPTGILNGPTVDSSLPTNSVSGQQPQLPLTRMPSVSQPGVHTACPRQTLANGPFSAGHVPCSTSRTLGSTDTVLIGNNHVTGSGSNGNVPYLQRNAPTLPHNRTNLTSSTEEPWKNQLSNSTQGLHKGPSSHLAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNTLTVPETSRQTGETPNSTASVEGLPNHVHQVMADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGPGTCDKVNNIHPTVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPIKTDLLLVSHRPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGKRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQNVKSIVPMVHLSWNMARNIKVSDPKLFEMIKYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLVSEINMLLHYHPPHLDIVPWTLNMRPFLLFRK

Target ORF information:

RefSeq Version	XM_006527599.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus lysine (K)-specific demethylase 6A (Kdm6a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527599.4

ORF Insert Sequence:

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCCG CCGCCGCCGC CGCCGCTTTC 
GGTGATGAGG AAAAGAAAAT GGCGGCGGGA AAAGCGAGCG GCGAGAGCGA GGAGGCGTCC 
CCCAGCCTGA CAGCGGAGGA GAGGGAGGCG CTCGGCGGAC TGGACAGCCG CCTTTTCGGG 
TTCGTGAGGT TTCATGAAGA TGGCGCCAGG ATGAAGGCCC TGCTGGGCAA GGCTGTTCGC 
TGCTACGAAT CTCTAATCTT AAAAGCTGAA GGGAAAGTGG AGTCTGATTT CTTTTGTCAA 
TTAGGTCACT TCAACCTCTT ATTGGAAGAT TATCCAAAAG CATTATCTGC ATACCAGAGG 
TACTACAGTT TACAGTCTGA TTACTGGAAG AATGCTGCCT TTTTATATGG TCTTGGTTTG 
GTCTACTTCC ATTACAATGC ATTTCAGTGG GCTATTAAAG CATTTCAGGA GGTGCTTTAT 
GTCGATCCCA GCTTTTGTCG AGCCAAGGAA ATTCATTTAC GACTTGGGCT TATGTTCAAA 
GTGAACACAG ACTATGAGTC TAGTTTAAAG CATTTTCAGT TAGCTTTGGT TGACTGTAAT 
CCCTGCACTT TGTCCAATGC TGAAATTCAG TTTCACATTG CCCACTTATA TGAAACCCAG 
AGGAAGTATC ATTCTGCAAA AGAAGCTTAT GAGCAACTTT TGCAGACAGA AAACCTTTCT 
GCACAAGTAA AAGCAACTAT TTTACAACAA TTAGGTTGGA TGCATCACAC TGTGGATCTC 
CTGGGAGATA AGGCCACCAA GGAAAGTTAT GCTATTCAGT ATCTCCAGAA GTCCTTGGAA 
GCAGATCCAA ATTCTGGCCA GTCCTGGTAT TTCCTTGGAA GGTGCTATTC AAGTATTGGG 
AAAGTTCAGG ATGCCTTTAT ATCTTACAGG CAATCTATTG ATAAATCAGA AGCAAGTGCA 
GATACATGGT GTTCAATAGG TGTGCTCTAT CAACAGCAAA ATCAGCCTAT GGATGCTTTG 
CAAGCTTATA TTTGTGCTGT ACAATTGGAC CACGGTCATG CTGCAGCCTG GATGGATCTA 
GGCACTCTCT ATGAATCCTG CAACCAACCT CAGGATGCTA TTAAATGCTA TTTAAATGCA 
ACTAGAAGCA AAAATTGTAG TAATACCTCT GGACTTGCAG CACGAATTAA GTATTTACAG 
AATACTTCTG ATAATTGGAG TGGTGGCAAT GCACCACCTC CAGTAGAACA ACAAACTCAT 
TCATGGTGTT TGACACCACA GAAATTACAG CACTTGGAAC AGCTCCGAGC AAACAGAAAT 
AATTTAAATC CAGCACAGAA ACTAATGCTG GAACAGCTGG AAAGTCAGTT TGTCTTAATG 
CAGCAACACC AAATGAGACA AACAGGAGTT GCACAGGTAC GGCCTACTGG AATTCTTAAT 
GGGCCAACAG TTGACTCATC ACTGCCTACA AACTCAGTTT CTGGCCAGCA GCCACAGCTT 
CCTCTGACCA GAATGCCTAG TGTCTCTCAG CCTGGAGTCC ACACTGCCTG TCCTAGGCAG 
ACTTTGGCCA ATGGACCCTT TTCTGCAGGC CATGTTCCCT GTAGCACATC AAGAACACTG 
GGAAGTACAG ACACTGTTTT GATAGGCAAT AATCATGTAA CAGGAAGTGG AAGTAATGGA 
AACGTGCCTT ACCTGCAGCG AAACGCACCC ACTCTACCTC ATAACCGCAC AAACCTGACC 
AGCAGCACAG AGGAGCCGTG GAAAAACCAA CTATCTAACT CCACTCAGGG GCTTCACAAA 
GGTCCGAGTT CACATTTGGC AGGTCCTAAT GGTGAACGAC CTCTATCTTC CACTGGGCCT 
TCCCAGCATC TCCAGGCAGC TGGCTCTGGT ATTCAGAATC AGAATGGACA TCCCACCCTG 
CCTAGCAATT CAGTAACACA GGGGGCTGCT CTCAATCACC TCTCCTCTCA CACTGCTACC 
TCAGGTGGAC AACAAGGCAT TACCTTAACC AAAGAGAGCA AGCCTTCAGG AAACACATTG 
ACGGTGCCTG AAACAAGCAG GCAAACTGGA GAGACACCTA ACAGCACTGC CAGTGTTGAG 
GGACTTCCTA ATCATGTCCA TCAGGTGATG GCAGATGCTG TTTGCAGTCC TAGCCATGGA 
GATTCTAAGT CACCAGGTTT ACTAAGTTCA GACAATCCTC AGCTCTCTGC CTTGTTGATG 
GGAAAAGCTA ATAACAATGT GGGTCCTGGA ACCTGTGACA AAGTCAATAA CATCCACCCA 
ACTGTCCATA CAAAGACTGA TAATTCTGTT GCCTCTTCAC CATCTTCAGC CATTTCCACA 
GCAACACCTT CTCCTAAGTC CACTGAACAG ACAACCACAA ACAGTGTTAC CAGCCTTAAC 
AGCCCTCACA GTGGGCTGCA CACAATTAAT GGAGAAGGAA TGGAAGAATC TCAGAGCCCC 
ATTAAAACAG ATCTGCTTCT AGTTAGCCAC AGACCTAGTC CTCAGATCAT ACCATCAATG 
TCTGTGTCCA TATATCCCAG CTCAGCAGAA GTTCTGAAAG CTTGCAGGAA TCTAGGTAAA 
AACGGCCTGT CTAATAGTAG CATTCTGTTG GATAAATGTC CGCCTCCAAG ACCACCATCC 
TCACCATACC CTCCCTTGCC AAAGGACAAG TTGAATCCAC CTACACCTAG TATTTATTTG 
GAAAATAAAC GTGATGCTTT CTTTCCTCCA TTACATCAAT TTTGTACAAA CCCAAACAAC 
CCTGTTACAG TAATACGTGG CCTTGCTGGA GCTCTTAAAT TAGACTTGGG ACTTTTCTCT 
ACTAAAACTT TGGTGGAAGC TAACAATGAA CATATGGTAG AAGTGAGGAC ACAGTTGTTA 
CAACCAGCAG ATGAAAATTG GGACCCTACT GGAACCAAGA AAATCTGGCA CTGTGAAAGT 
AATAGATCTC ATACTACAAT TGCTAAATAT GCTCAGTACC AGGCCTCCTC ATTCCAAGAA 
TCATTGAGAG AAGAAAATGA GAAAAGAAGT CACCATAAAG ACCACTCAGA CAGTGAATCT 
ACATCATCAG ATAATTCTGG GAAAAGAAGA AAAGGACCCT TTAAAACCAT TAAGTTTGGG 
ACCAACATTG ACCTGTCTGA TGACAAAAAG TGGAAGTTAC AGCTACATGA GCTGACTAAA 
CTTCCTGCCT TCGTGAGAGT TGTATCTGCA GGAAATCTTT TAAGCCACGT TGGTCATACT 
ATACTGGGCA TGAACACAGT TCAACTATAC ATGAAAGTTC CAGGAAGCAG AACACCAGGT 
CATCAAGAAA ATAACAACTT CTGTTCAGTT AATATAAATA TTGGCCCAGG TGACTGTGAA 
TGGTTTGTTG TTCCTGAAGG CTACTGGGGT GTTTTGAATG ACTTCTGTGA AAAAAATAAT 
TTGAATTTCT TAATGGGTTC TTGGTGGCCC AACCTTGAAG ATCTATATGA AGCAAATGTT 
CCAGTGTATA GGTTTATTCA GCGACCTGGA GATCTGGTCT GGATAAATGC TGGCACTGTT 
CATTGGGTTC AAGCTATTGG CTGGTGCAAC AATATTGCTT GGAATGTTGG TCCACTTACA 
GCCTGTCAGT ATAAGTTAGC AGTGGAACGT TATGAATGGA ACAAGTTGCA AAATGTAAAG 
TCAATAGTAC CCATGGTTCA TCTTTCCTGG AATATGGCAC GAAATATCAA GGTTTCAGAT 
CCAAAGCTTT TTGAAATGAT TAAGTATTGT CTTCTGAGAA CGCTGAAGCA ATGTCAGACA 
TTGAGGGAAG CTCTAATTGC TGCAGGAAAA GAGATCATAT GGCACGGGCG GACAAAAGAA 
GAACCAGCTC ATTATTGTAG TATTTGTGAG GTGGAGGTTT TTGATCTGCT TTTTGTCACT 
AATGAGAGTA ATTCTCGAAA AACCTACATA GTACATTGCC AAGATTGTGC ACGAAAAACA 
AGTGGGAATC TGGAAAATTT TGTGGTGCTA GAACAGTACA AAATGGAGGA TCTGATGCAA 
GTCTATGACC AATTTACATT AGTAAGTGAA ATCAACATGC TCCTCCATTA CCATCCGCCT 
CATCTTGATA TTGTTCCATG GACATTAAAC ATGAGACCTT TTCTGCTATT CAGAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kdm6a ( XM_006527600.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527663.1 Mus musculus lysine (K)-specific demethylase 6A (Kdm6a), transcript variant X7, mRNA.

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CloneID	OMu55897
Clone ID Related Accession (Same CDS sequence)	XM_006527600.3 , XM_006527600.4
Accession Version	XM_006527600.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	lysine-specific demethylase 6A isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006527600.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCCG CCGCCGCCGC CGCCGCTTTC 
GGTGATGAGG AAAAGAAAAT GGCGGCGGGA AAAGCGAGCG GCGAGAGCGA GGAGGCGTCC 
CCCAGCCTGA CAGCGGAGGA GAGGGAGGCG CTCGGCGGAC TGGACAGCCG CCTTTTCGGG 
TTCGTGAGGT TTCATGAAGA TGGCGCCAGG ATGAAGGCCC TGCTGGGCAA GGCTGTTCGC 
TGCTACGAAT CTCTAATCTT AAAAGCTGAA GGGAAAGTGG AGTCTGATTT CTTTTGTCAA 
TTAGGTCACT TCAACCTCTT ATTGGAAGAT TATCCAAAAG CATTATCTGC ATACCAGAGG 
TACTACAGTT TACAGTCTGA TTACTGGAAG AATGCTGCCT TTTTATATGG TCTTGGTTTG 
GTCTACTTCC ATTACAATGC ATTTCAGTGG GCTATTAAAG CATTTCAGGA GGTGCTTTAT 
GTCGATCCCA GCTTTTGTCG AGCCAAGGAA ATTCATTTAC GACTTGGGCT TATGTTCAAA 
GTGAACACAG ACTATGAGTC TAGTTTAAAG CATTTTCAGT TAGCTTTGGT TGACTGTAAT 
CCCTGCACTT TGTCCAATGC TGAAATTCAG TTTCACATTG CCCACTTATA TGAAACCCAG 
AGGAAGTATC ATTCTGCAAA AGAAGCTTAT GAGCAACTTT TGCAGACAGA AAACCTTTCT 
GCACAAGTAA AAGCAACTAT TTTACAACAA TTAGGTTGGA TGCATCACAC TGTGGATCTC 
CTGGGAGATA AGGCCACCAA GGAAAGTTAT GCTATTCAGT ATCTCCAGAA GTCCTTGGAA 
GCAGATCCAA ATTCTGGCCA GTCCTGGTAT TTCCTTGGAA GGTGCTATTC AAGTATTGGG 
AAAGTTCAGG ATGCCTTTAT ATCTTACAGG CAATCTATTG ATAAATCAGA AGCAAGTGCA 
GATACATGGT GTTCAATAGG TGTGCTCTAT CAACAGCAAA ATCAGCCTAT GGATGCTTTG 
CAAGCTTATA TTTGTGCTGT ACAATTGGAC CACGGTCATG CTGCAGCCTG GATGGATCTA 
GGCACTCTCT ATGAATCCTG CAACCAACCT CAGGATGCTA TTAAATGCTA TTTAAATGCA 
ACTAGAAGCA AAAATTGTAG TAATACCTCT GGACTTGCAG CACGAATTAA GTATTTACAG 
AATACTTCTG ATAATTGGAG TGGTGGCAAT GCACCACCTC CAGTAGAACA ACAAACTCAT 
TCATGGTGTT TGACACCACA GAAATTACAG CACTTGGAAC AGCTCCGAGC AAACAGAAAT 
AATTTAAATC CAGCACAGAA ACTAATGCTG GAACAGCTGG AAAGTCAGTT TGTCTTAATG 
CAGCAACACC AAATGAGACA AACAGGAGTT GCACAGGTAC GGCCTACTGG AATTCTTAAT 
GGGCCAACAG TTGACTCATC ACTGCCTACA AACTCAGTTT CTGGCCAGCA GCCACAGCTT 
CCTCTGACCA GAATGCCTAG TGTCTCTCAG CCTGGAGTCC ACACTGCCTG TCCTAGGCAG 
ACTTTGGCCA ATGGACCCTT TTCTGCAGGC CATGTTCCCT GTAGCACATC AAGAACACTG 
GGAAGTACAG ACACTGTTTT GATAGGCAAT AATCATGTAA CAGGAAGTGG AAGTAATGGA 
AACGTGCCTT ACCTGCAGCG AAACGCACCC ACTCTACCTC ATAACCGCAC AAACCTGACC 
AGCAGCACAG AGGAGCCGTG GAAAAACCAA CTATCTAACT CCACTCAGGG GCTTCACAAA 
GGTCCGAGTT CACATTTGGC AGGTCCTAAT GGTGAACGAC CTCTATCTTC CACTGGGCCT 
TCCCAGCATC TCCAGGCAGC TGGCTCTGGT ATTCAGAATC AGAATGGACA TCCCACCCTG 
CCTAGCAATT CAGTAACACA GGGGGCTGCT CTCAATCACC TCTCCTCTCA CACTGCTACC 
TCAGGTGGAC AACAAGGCAT TACCTTAACC AAAGAGAGCA AGCCTTCAGG AAACACATTG 
ACGGTGCCTG AAACAAGCAG GCAAACTGGA GAGACACCTA ACAGCACTGC CAGTGTTGAG 
GGACTTCCTA ATCATGTCCA TCAGGTGATG GCAGATGCTG TTTGCAGTCC TAGCCATGGA 
GATTCTAAGT CACCAGGTTT ACTAAGTTCA GACAATCCTC AGCTCTCTGC CTTGTTGATG 
GGAAAAGCTA ATAACAATGT GGGTCCTGGA ACCTGTGACA AAGTCAATAA CATCCACCCA 
ACTGTCCATA CAAAGACTGA TAATTCTGTT GCCTCTTCAC CATCTTCAGC CATTTCCACA 
GCAACACCTT CTCCTAAGTC CACTGAACAG ACAACCACAA ACAGTGTTAC CAGCCTTAAC 
AGCCCTCACA GTGGGCTGCA CACAATTAAT GGAGAAGGAA TGGAAGAATC TCAGAGCCCC 
ATTAAAACAG ATCTGCTTCT AGTTAGCCAC AGACCTAGTC CTCAGATCAT ACCATCAATG 
TCTGTGTCCA TATATCCCAG CTCAGCAGAA GTTCTGAAAG CTTGCAGGAA TCTAGGTAAA 
AACGGCCTGT CTAATAGTAG CATTCTGTTG GATAAATGTC CGCCTCCAAG ACCACCATCC 
TCACCATACC CTCCCTTGCC AAAGGACAAG TTGAATCCAC CTACACCTAG TATTTATTTG 
GAAAATAAAC GTGATGCTTT CTTTCCTCCA TTACATCAAT TTTGTACAAA CCCAAACAAC 
CCTGTTACAG TAATACGTGG CCTTGCTGGA GCTCTTAAAT TAGACTTGGG ACTTTTCTCT 
ACTAAAACTT TGGTGGAAGC TAACAATGAA CATATGGTAG AAGTGAGGAC ACAGTTGTTA 
CAACCAGCAG ATGAAAATTG GGACCCTACT GGAACCAAGA AAATCTGGCA CTGTGAAAGT 
AATAGATCTC ATACTACAAT TGCTAAATAT GCTCAGTACC AGGCCTCCTC ATTCCAAGAA 
TCATTGAGAG AAGAAAATGA GAAAAGAAGT CACCATAAAG ACCACTCAGA CAGTGAATCT 
ACATCATCAG ATAATTCTGG GAAAAGAAGA AAAGGACCCT TTAAAACCAT TAAGTTTGGG 
ACCAACATTG ACCTGTCTGA TGACAAAAAG TGGAAGTTAC AGCTACATGA GCTGACTAAA 
CTTCCTGCCT TCGTGAGAGT TGTATCTGCA GGAAATCTTT TAAGCCACGT TGGTCATACT 
ATACTGGGCA TGAACACAGT TCAACTATAC ATGAAAGTTC CAGGAAGCAG AACACCAGGT 
CATCAAGAAA ATAACAACTT CTGTTCAGTT AATATAAATA TTGGCCCAGG TGACTGTGAA 
TGGTTTGTTG TTCCTGAAGG CTACTGGGGT GTTTTGAATG ACTTCTGTGA AAAAAATAAT 
TTGAATTTCT TAATGGGTTC TTGGTGGCCC AACCTTGAAG ATCTATATGA AGCAAATGTT 
CCAGTGTATA GGTTTATTCA GCGACCTGGA GATCTGGTCT GGATAAATGC TGGCACTGTT 
CATTGGGTTC AAGCTATTGG CTGGTGCAAC AATATTGCTT GGAATGTTGG TCCACTTACA 
GCCTGTCAGT ATAAGTTAGC AGTGGAACGT TATGAATGGA ACAAGTTGCA AAATGTAAAG 
TCAATAGTAC CCATGGTTCA TCTTTCCTGG AATATGGCAC GAAATATCAA GGTTTCAGAT 
CCAAAGCTTT TTGAAATGAT TAAGTATTGT CTTCTGAGAA CGCTGAAGCA ATGTCAGACA 
TTGAGGGAAG CTCTAATTGC TGCAGGAAAA GAGATCATAT GGCACGGGCG GACAAAAGAA 
GAACCAGCTC ATTATTGTAG TATTTGTGAG GTGGAGGTTT TTGATCTGCT TTTTGTCACT 
AATGAGAGTA ATTCTCGAAA AACCTACATA GTACATTGCC AAGATTGTGC ACGAAAAACA 
AGTGGGAATC TGGAAAATTT TGTGGTGCTA GAACAGTACA AAATGGAGGA TCTGATGCAA 
GTCTATGACC AATTTACATT AGCTCCTCCA TTACCATCCG CCTCATCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006527663.1
CDS	2145..6215
Translation	MKSCGVSLATAAAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARMKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATILQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKNCSNTSGLAARIKYLQNTSDNWSGGNAPPPVEQQTHSWCLTPQKLQHLEQLRANRNNLNPAQKLMLEQLESQFVLMQQHQMRQTGVAQVRPTGILNGPTVDSSLPTNSVSGQQPQLPLTRMPSVSQPGVHTACPRQTLANGPFSAGHVPCSTSRTLGSTDTVLIGNNHVTGSGSNGNVPYLQRNAPTLPHNRTNLTSSTEEPWKNQLSNSTQGLHKGPSSHLAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNTLTVPETSRQTGETPNSTASVEGLPNHVHQVMADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGPGTCDKVNNIHPTVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPIKTDLLLVSHRPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGKRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQNVKSIVPMVHLSWNMARNIKVSDPKLFEMIKYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLAPPLPSASS

Target ORF information:

RefSeq Version	XM_006527600.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus lysine (K)-specific demethylase 6A (Kdm6a), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527600.4

ORF Insert Sequence:

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCCG CCGCCGCCGC CGCCGCTTTC 
GGTGATGAGG AAAAGAAAAT GGCGGCGGGA AAAGCGAGCG GCGAGAGCGA GGAGGCGTCC 
CCCAGCCTGA CAGCGGAGGA GAGGGAGGCG CTCGGCGGAC TGGACAGCCG CCTTTTCGGG 
TTCGTGAGGT TTCATGAAGA TGGCGCCAGG ATGAAGGCCC TGCTGGGCAA GGCTGTTCGC 
TGCTACGAAT CTCTAATCTT AAAAGCTGAA GGGAAAGTGG AGTCTGATTT CTTTTGTCAA 
TTAGGTCACT TCAACCTCTT ATTGGAAGAT TATCCAAAAG CATTATCTGC ATACCAGAGG 
TACTACAGTT TACAGTCTGA TTACTGGAAG AATGCTGCCT TTTTATATGG TCTTGGTTTG 
GTCTACTTCC ATTACAATGC ATTTCAGTGG GCTATTAAAG CATTTCAGGA GGTGCTTTAT 
GTCGATCCCA GCTTTTGTCG AGCCAAGGAA ATTCATTTAC GACTTGGGCT TATGTTCAAA 
GTGAACACAG ACTATGAGTC TAGTTTAAAG CATTTTCAGT TAGCTTTGGT TGACTGTAAT 
CCCTGCACTT TGTCCAATGC TGAAATTCAG TTTCACATTG CCCACTTATA TGAAACCCAG 
AGGAAGTATC ATTCTGCAAA AGAAGCTTAT GAGCAACTTT TGCAGACAGA AAACCTTTCT 
GCACAAGTAA AAGCAACTAT TTTACAACAA TTAGGTTGGA TGCATCACAC TGTGGATCTC 
CTGGGAGATA AGGCCACCAA GGAAAGTTAT GCTATTCAGT ATCTCCAGAA GTCCTTGGAA 
GCAGATCCAA ATTCTGGCCA GTCCTGGTAT TTCCTTGGAA GGTGCTATTC AAGTATTGGG 
AAAGTTCAGG ATGCCTTTAT ATCTTACAGG CAATCTATTG ATAAATCAGA AGCAAGTGCA 
GATACATGGT GTTCAATAGG TGTGCTCTAT CAACAGCAAA ATCAGCCTAT GGATGCTTTG 
CAAGCTTATA TTTGTGCTGT ACAATTGGAC CACGGTCATG CTGCAGCCTG GATGGATCTA 
GGCACTCTCT ATGAATCCTG CAACCAACCT CAGGATGCTA TTAAATGCTA TTTAAATGCA 
ACTAGAAGCA AAAATTGTAG TAATACCTCT GGACTTGCAG CACGAATTAA GTATTTACAG 
AATACTTCTG ATAATTGGAG TGGTGGCAAT GCACCACCTC CAGTAGAACA ACAAACTCAT 
TCATGGTGTT TGACACCACA GAAATTACAG CACTTGGAAC AGCTCCGAGC AAACAGAAAT 
AATTTAAATC CAGCACAGAA ACTAATGCTG GAACAGCTGG AAAGTCAGTT TGTCTTAATG 
CAGCAACACC AAATGAGACA AACAGGAGTT GCACAGGTAC GGCCTACTGG AATTCTTAAT 
GGGCCAACAG TTGACTCATC ACTGCCTACA AACTCAGTTT CTGGCCAGCA GCCACAGCTT 
CCTCTGACCA GAATGCCTAG TGTCTCTCAG CCTGGAGTCC ACACTGCCTG TCCTAGGCAG 
ACTTTGGCCA ATGGACCCTT TTCTGCAGGC CATGTTCCCT GTAGCACATC AAGAACACTG 
GGAAGTACAG ACACTGTTTT GATAGGCAAT AATCATGTAA CAGGAAGTGG AAGTAATGGA 
AACGTGCCTT ACCTGCAGCG AAACGCACCC ACTCTACCTC ATAACCGCAC AAACCTGACC 
AGCAGCACAG AGGAGCCGTG GAAAAACCAA CTATCTAACT CCACTCAGGG GCTTCACAAA 
GGTCCGAGTT CACATTTGGC AGGTCCTAAT GGTGAACGAC CTCTATCTTC CACTGGGCCT 
TCCCAGCATC TCCAGGCAGC TGGCTCTGGT ATTCAGAATC AGAATGGACA TCCCACCCTG 
CCTAGCAATT CAGTAACACA GGGGGCTGCT CTCAATCACC TCTCCTCTCA CACTGCTACC 
TCAGGTGGAC AACAAGGCAT TACCTTAACC AAAGAGAGCA AGCCTTCAGG AAACACATTG 
ACGGTGCCTG AAACAAGCAG GCAAACTGGA GAGACACCTA ACAGCACTGC CAGTGTTGAG 
GGACTTCCTA ATCATGTCCA TCAGGTGATG GCAGATGCTG TTTGCAGTCC TAGCCATGGA 
GATTCTAAGT CACCAGGTTT ACTAAGTTCA GACAATCCTC AGCTCTCTGC CTTGTTGATG 
GGAAAAGCTA ATAACAATGT GGGTCCTGGA ACCTGTGACA AAGTCAATAA CATCCACCCA 
ACTGTCCATA CAAAGACTGA TAATTCTGTT GCCTCTTCAC CATCTTCAGC CATTTCCACA 
GCAACACCTT CTCCTAAGTC CACTGAACAG ACAACCACAA ACAGTGTTAC CAGCCTTAAC 
AGCCCTCACA GTGGGCTGCA CACAATTAAT GGAGAAGGAA TGGAAGAATC TCAGAGCCCC 
ATTAAAACAG ATCTGCTTCT AGTTAGCCAC AGACCTAGTC CTCAGATCAT ACCATCAATG 
TCTGTGTCCA TATATCCCAG CTCAGCAGAA GTTCTGAAAG CTTGCAGGAA TCTAGGTAAA 
AACGGCCTGT CTAATAGTAG CATTCTGTTG GATAAATGTC CGCCTCCAAG ACCACCATCC 
TCACCATACC CTCCCTTGCC AAAGGACAAG TTGAATCCAC CTACACCTAG TATTTATTTG 
GAAAATAAAC GTGATGCTTT CTTTCCTCCA TTACATCAAT TTTGTACAAA CCCAAACAAC 
CCTGTTACAG TAATACGTGG CCTTGCTGGA GCTCTTAAAT TAGACTTGGG ACTTTTCTCT 
ACTAAAACTT TGGTGGAAGC TAACAATGAA CATATGGTAG AAGTGAGGAC ACAGTTGTTA 
CAACCAGCAG ATGAAAATTG GGACCCTACT GGAACCAAGA AAATCTGGCA CTGTGAAAGT 
AATAGATCTC ATACTACAAT TGCTAAATAT GCTCAGTACC AGGCCTCCTC ATTCCAAGAA 
TCATTGAGAG AAGAAAATGA GAAAAGAAGT CACCATAAAG ACCACTCAGA CAGTGAATCT 
ACATCATCAG ATAATTCTGG GAAAAGAAGA AAAGGACCCT TTAAAACCAT TAAGTTTGGG 
ACCAACATTG ACCTGTCTGA TGACAAAAAG TGGAAGTTAC AGCTACATGA GCTGACTAAA 
CTTCCTGCCT TCGTGAGAGT TGTATCTGCA GGAAATCTTT TAAGCCACGT TGGTCATACT 
ATACTGGGCA TGAACACAGT TCAACTATAC ATGAAAGTTC CAGGAAGCAG AACACCAGGT 
CATCAAGAAA ATAACAACTT CTGTTCAGTT AATATAAATA TTGGCCCAGG TGACTGTGAA 
TGGTTTGTTG TTCCTGAAGG CTACTGGGGT GTTTTGAATG ACTTCTGTGA AAAAAATAAT 
TTGAATTTCT TAATGGGTTC TTGGTGGCCC AACCTTGAAG ATCTATATGA AGCAAATGTT 
CCAGTGTATA GGTTTATTCA GCGACCTGGA GATCTGGTCT GGATAAATGC TGGCACTGTT 
CATTGGGTTC AAGCTATTGG CTGGTGCAAC AATATTGCTT GGAATGTTGG TCCACTTACA 
GCCTGTCAGT ATAAGTTAGC AGTGGAACGT TATGAATGGA ACAAGTTGCA AAATGTAAAG 
TCAATAGTAC CCATGGTTCA TCTTTCCTGG AATATGGCAC GAAATATCAA GGTTTCAGAT 
CCAAAGCTTT TTGAAATGAT TAAGTATTGT CTTCTGAGAA CGCTGAAGCA ATGTCAGACA 
TTGAGGGAAG CTCTAATTGC TGCAGGAAAA GAGATCATAT GGCACGGGCG GACAAAAGAA 
GAACCAGCTC ATTATTGTAG TATTTGTGAG GTGGAGGTTT TTGATCTGCT TTTTGTCACT 
AATGAGAGTA ATTCTCGAAA AACCTACATA GTACATTGCC AAGATTGTGC ACGAAAAACA 
AGTGGGAATC TGGAAAATTT TGTGGTGCTA GAACAGTACA AAATGGAGGA TCTGATGCAA 
GTCTATGACC AATTTACATT AGCTCCTCCA TTACCATCCG CCTCATCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kdm6a ( XM_017318461.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173950.1 Mus musculus lysine (K)-specific demethylase 6A (Kdm6a), transcript variant X3, mRNA.

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CloneID	OMu83109
Clone ID Related Accession (Same CDS sequence)	XM_017318461.1 , XM_017318461.2
Accession Version	XM_017318461.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4332bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	lysine-specific demethylase 6A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017318461.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCCG CCGCCGCCGC CGCCGCTTTC 
GGTGATGAGG AAAAGAAAAT GGCGGCGGGA AAAGCGAGCG GCGAGAGCGA GGAGGCGTCC 
CCCAGCCTGA CAGCGGAGGA GAGGGAGGCG CTCGGCGGAC TGGACAGCCG CCTTTTCGGG 
TTCGTGAGGT TTCATGAAGA TGGCGCCAGG ATGAAGGCCC TGCTGGGCAA GGCTGTTCGC 
TGCTACGAAT CTCTAATCTT AAAAGCTGAA GGGAAAGTGG AGTCTGATTT CTTTTGTCAA 
TTAGGTCACT TCAACCTCTT ATTGGAAGAT TATCCAAAAG CATTATCTGC ATACCAGAGG 
TACTACAGTT TACAGTCTGA TTACTGGAAG AATGCTGCCT TTTTATATGG TCTTGGTTTG 
GTCTACTTCC ATTACAATGC ATTTCAGTGG GCTATTAAAG CATTTCAGGA GGTGCTTTAT 
GTCGATCCCA GCTTTTGTCG AGCCAAGGAA ATTCATTTAC GACTTGGGCT TATGTTCAAA 
GTGAACACAG ACTATGAGTC TAGTTTAAAG CATTTTCAGT TAGCTTTGGT TGACTGTAAT 
CCCTGCACTT TGTCCAATGC TGAAATTCAG TTTCACATTG CCCACTTATA TGAAACCCAG 
AGGAAGTATC ATTCTGCAAA AGAAGCTTAT GAGCAACTTT TGCAGACAGA AAACCTTTCT 
GCACAAGTAA AAGCAACTAT TTTACAACAA TTAGGTTGGA TGCATCACAC TGTGGATCTC 
CTGGGAGATA AGGCCACCAA GGAAAGTTAT GCTATTCAGT ATCTCCAGAA GTCCTTGGAA 
GCAGATCCAA ATTCTGGCCA GTCCTGGTAT TTCCTTGGAA GTGTGCTCTA TCAACAGCAA 
AATCAGCCTA TGGATGCTTT GCAAGCTTAT ATTTGTGCTG TACAATTGGA CCACGGTCAT 
GCTGCAGCCT GGATGGATCT AGGCACTCTC TATGAATCCT GCAACCAACC TCAGGATGCT 
ATTAAATGCT ATTTAAATGC AACTAGAAGC AAAAATTGTA GTAATACCTC TGGACTTGCA 
GCACGAATTA AGTATTTACA GGCTCAGTTG TGTAACCTTC CACAAGGTAG TCTACAGAAT 
AAAACTAAAT TACTTCCTAG TATTGAGGAG GCATGGAGCC TACCAATCCC CGCAGAGCTT 
ACCTCCAGGC AGGGTGCCAT GAACACAGCA CAGCAGGCAT GTAAACCTCA TCATCCAAAT 
ACTGAACCTG TATTAGGCCT CAGTCAAACA CCAATTTCAC AGCAGTCCTT GCCACTACAC 
ATGATTCCTC CTAGCCAAGT AGATGACCTG TCCAGTCCTG CCAAGAGGAA AAGAACATCT 
AGTCCAACAA AGAATACTTC TGATAATTGG AGTGGTGGCA ATGCACCACC TCCAGTAGAA 
CAACAAACTC ATTCATGGTG TTTGACACCA CAGAAATTAC AGCACTTGGA ACAGCTCCGA 
GCAAACAGAA ATAATTTAAA TCCAGCACAG AAACTAATGC TGGAACAGCT GGAAAGTCAG 
TTTGTCTTAA TGCAGCAACA CCAAATGAGA CAAACAGGAG TTGCACAGGT ACGGCCTACT 
GGAATTCTTA ATGGGCCAAC AGTTGACTCA TCACTGCCTA CAAACTCAGT TTCTGGCCAG 
CAGCCACAGC TTCCTCTGAC CAGAATGCCT AGTGTCTCTC AGCCTGGAGT CCACACTGCC 
TGTCCTAGGC AGACTTTGGC CAATGGACCC TTTTCTGCAG GCCATGTTCC CTGTAGCACA 
TCAAGAACAC TGGGAAGTAC AGACACTGTT TTGATAGGCA ATAATCATGT AACAGGAAGT 
GGAAGTAATG GAAACGTGCC TTACCTGCAG CGAAACGCAC CCACTCTACC TCATAACCGC 
ACAAACCTGA CCAGCAGCAC AGAGGAGCCG TGGAAAAACC AACTATCTAA CTCCACTCAG 
GGGCTTCACA AAGGTCCGAG TTCACATTTG GCAGGTCCTA ATGGTGAACG ACCTCTATCT 
TCCACTGGGC CTTCCCAGCA TCTCCAGGCA GCTGGCTCTG GTATTCAGAA TCAGAATGGA 
CATCCCACCC TGCCTAGCAA TTCAGTAACA CAGGGGGCTG CTCTCAATCA CCTCTCCTCT 
CACACTGCTA CCTCAGGTGG ACAACAAGGC ATTACCTTAA CCAAAGAGAG CAAGCCTTCA 
GGAAACACAT TGACGGTGCC TGAAACAAGC AGGCAAACTG GAGAGACACC TAACAGCACT 
GCCAGTGTTG AGGGACTTCC TAATCATGTC CATCAGGTGA TGGCAGATGC TGTTTGCAGT 
CCTAGCCATG GAGATTCTAA GTCACCAGGT TTACTAAGTT CAGACAATCC TCAGCTCTCT 
GCCTTGTTGA TGGGAAAAGC TAATAACAAT GTGGGTCCTG GAACCTGTGA CAAAGTCAAT 
AACATCCACC CAACTGTCCA TACAAAGACT GATAATTCTG TTGCCTCTTC ACCATCTTCA 
GCCATTTCCA CAGCAACACC TTCTCCTAAG TCCACTGAAC AGACAACCAC AAACAGTGTT 
ACCAGCCTTA ACAGCCCTCA CAGTGGGCTG CACACAATTA ATGGAGAAGG AATGGAAGAA 
TCTCAGAGCC CCATTAAAAC AGATCTGCTT CTAGTTAGCC ACAGACCTAG TCCTCAGATC 
ATACCATCAA TGTCTGTGTC CATATATCCC AGCTCAGCAG AAGTTCTGAA AGCTTGCAGG 
AATCTAGGTA AAAACGGCCT GTCTAATAGT AGCATTCTGT TGGATAAATG TCCGCCTCCA 
AGACCACCAT CCTCACCATA CCCTCCCTTG CCAAAGGACA AGTTGAATCC ACCTACACCT 
AGTATTTATT TGGAAAATAA ACGTGATGCT TTCTTTCCTC CATTACATCA ATTTTGTACA 
AACCCAAACA ACCCTGTTAC AGTAATACGT GGCCTTGCTG GAGCTCTTAA ATTAGACTTG 
GGACTTTTCT CTACTAAAAC TTTGGTGGAA GCTAACAATG AACATATGGT AGAAGTGAGG 
ACACAGTTGT TACAACCAGC AGATGAAAAT TGGGACCCTA CTGGAACCAA GAAAATCTGG 
CACTGTGAAA GTAATAGATC TCATACTACA ATTGCTAAAT ATGCTCAGTA CCAGGCCTCC 
TCATTCCAAG AATCATTGAG AGAAGAAAAT GAGAAAAGAA GTCACCATAA AGACCACTCA 
GACAGTGAAT CTACATCATC AGATAATTCT GGGAAAAGAA GAAAAGGACC CTTTAAAACC 
ATTAAGTTTG GGACCAACAT TGACCTGTCT GATGACAAAA AGTGGAAGTT ACAGCTACAT 
GAGCTGACTA AACTTCCTGC CTTCGTGAGA GTTGTATCTG CAGGAAATCT TTTAAGCCAC 
GTTGGTCATA CTATACTGGG CATGAACACA GTTCAACTAT ACATGAAAGT TCCAGGAAGC 
AGAACACCAG GTCATCAAGA AAATAACAAC TTCTGTTCAG TTAATATAAA TATTGGCCCA 
GGTGACTGTG AATGGTTTGT TGTTCCTGAA GGCTACTGGG GTGTTTTGAA TGACTTCTGT 
GAAAAAAATA ATTTGAATTT CTTAATGGGT TCTTGGTGGC CCAACCTTGA AGATCTATAT 
GAAGCAAATG TTCCAGTGTA TAGGTTTATT CAGCGACCTG GAGATCTGGT CTGGATAAAT 
GCTGGCACTG TTCATTGGGT TCAAGCTATT GGCTGGTGCA ACAATATTGC TTGGAATGTT 
GGTCCACTTA CAGCCTGTCA GTATAAGTTA GCAGTGGAAC GTTATGAATG GAACAAGTTG 
CAAAATGTAA AGTCAATAGT ACCCATGGTT CATCTTTCCT GGAATATGGC ACGAAATATC 
AAGGTTTCAG ATCCAAAGCT TTTTGAAATG ATTAAGTATT GTCTTCTGAG AACGCTGAAG 
CAATGTCAGA CATTGAGGGA AGCTCTAATT GCTGCAGGAA AAGAGATCAT ATGGCACGGG 
CGGACAAAAG AAGAACCAGC TCATTATTGT AGTATTTGTG AGGTGGAGGT TTTTGATCTG 
CTTTTTGTCA CTAATGAGAG TAATTCTCGA AAAACCTACA TAGTACATTG CCAAGATTGT 
GCACGAAAAA CAAGTGGGAA TCTGGAAAAT TTTGTGGTGC TAGAACAGTA CAAAATGGAG 
GATCTGATGC AAGTCTATGA CCAATTTACA TTAGTAAGTG AAATCAACAT GCTCCTCCAT 
TACCATCCGC CTCATCTTGA TATTGTTCCA TGGACATTAA ACATGAGACC TTTTCTGCTA 
TTCAGAAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017173950.1
CDS	2145..6476
Translation	MKSCGVSLATAAAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARMKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATILQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGSVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKNCSNTSGLAARIKYLQAQLCNLPQGSLQNKTKLLPSIEEAWSLPIPAELTSRQGAMNTAQQACKPHHPNTEPVLGLSQTPISQQSLPLHMIPPSQVDDLSSPAKRKRTSSPTKNTSDNWSGGNAPPPVEQQTHSWCLTPQKLQHLEQLRANRNNLNPAQKLMLEQLESQFVLMQQHQMRQTGVAQVRPTGILNGPTVDSSLPTNSVSGQQPQLPLTRMPSVSQPGVHTACPRQTLANGPFSAGHVPCSTSRTLGSTDTVLIGNNHVTGSGSNGNVPYLQRNAPTLPHNRTNLTSSTEEPWKNQLSNSTQGLHKGPSSHLAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNTLTVPETSRQTGETPNSTASVEGLPNHVHQVMADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGPGTCDKVNNIHPTVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPIKTDLLLVSHRPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGKRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQNVKSIVPMVHLSWNMARNIKVSDPKLFEMIKYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLVSEINMLLHYHPPHLDIVPWTLNMRPFLLFRK

Target ORF information:

RefSeq Version	XM_017318461.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus lysine (K)-specific demethylase 6A (Kdm6a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017318461.2

ORF Insert Sequence:

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCCG CCGCCGCCGC CGCCGCTTTC 
GGTGATGAGG AAAAGAAAAT GGCGGCGGGA AAAGCGAGCG GCGAGAGCGA GGAGGCGTCC 
CCCAGCCTGA CAGCGGAGGA GAGGGAGGCG CTCGGCGGAC TGGACAGCCG CCTTTTCGGG 
TTCGTGAGGT TTCATGAAGA TGGCGCCAGG ATGAAGGCCC TGCTGGGCAA GGCTGTTCGC 
TGCTACGAAT CTCTAATCTT AAAAGCTGAA GGGAAAGTGG AGTCTGATTT CTTTTGTCAA 
TTAGGTCACT TCAACCTCTT ATTGGAAGAT TATCCAAAAG CATTATCTGC ATACCAGAGG 
TACTACAGTT TACAGTCTGA TTACTGGAAG AATGCTGCCT TTTTATATGG TCTTGGTTTG 
GTCTACTTCC ATTACAATGC ATTTCAGTGG GCTATTAAAG CATTTCAGGA GGTGCTTTAT 
GTCGATCCCA GCTTTTGTCG AGCCAAGGAA ATTCATTTAC GACTTGGGCT TATGTTCAAA 
GTGAACACAG ACTATGAGTC TAGTTTAAAG CATTTTCAGT TAGCTTTGGT TGACTGTAAT 
CCCTGCACTT TGTCCAATGC TGAAATTCAG TTTCACATTG CCCACTTATA TGAAACCCAG 
AGGAAGTATC ATTCTGCAAA AGAAGCTTAT GAGCAACTTT TGCAGACAGA AAACCTTTCT 
GCACAAGTAA AAGCAACTAT TTTACAACAA TTAGGTTGGA TGCATCACAC TGTGGATCTC 
CTGGGAGATA AGGCCACCAA GGAAAGTTAT GCTATTCAGT ATCTCCAGAA GTCCTTGGAA 
GCAGATCCAA ATTCTGGCCA GTCCTGGTAT TTCCTTGGAA GTGTGCTCTA TCAACAGCAA 
AATCAGCCTA TGGATGCTTT GCAAGCTTAT ATTTGTGCTG TACAATTGGA CCACGGTCAT 
GCTGCAGCCT GGATGGATCT AGGCACTCTC TATGAATCCT GCAACCAACC TCAGGATGCT 
ATTAAATGCT ATTTAAATGC AACTAGAAGC AAAAATTGTA GTAATACCTC TGGACTTGCA 
GCACGAATTA AGTATTTACA GGCTCAGTTG TGTAACCTTC CACAAGGTAG TCTACAGAAT 
AAAACTAAAT TACTTCCTAG TATTGAGGAG GCATGGAGCC TACCAATCCC CGCAGAGCTT 
ACCTCCAGGC AGGGTGCCAT GAACACAGCA CAGCAGGCAT GTAAACCTCA TCATCCAAAT 
ACTGAACCTG TATTAGGCCT CAGTCAAACA CCAATTTCAC AGCAGTCCTT GCCACTACAC 
ATGATTCCTC CTAGCCAAGT AGATGACCTG TCCAGTCCTG CCAAGAGGAA AAGAACATCT 
AGTCCAACAA AGAATACTTC TGATAATTGG AGTGGTGGCA ATGCACCACC TCCAGTAGAA 
CAACAAACTC ATTCATGGTG TTTGACACCA CAGAAATTAC AGCACTTGGA ACAGCTCCGA 
GCAAACAGAA ATAATTTAAA TCCAGCACAG AAACTAATGC TGGAACAGCT GGAAAGTCAG 
TTTGTCTTAA TGCAGCAACA CCAAATGAGA CAAACAGGAG TTGCACAGGT ACGGCCTACT 
GGAATTCTTA ATGGGCCAAC AGTTGACTCA TCACTGCCTA CAAACTCAGT TTCTGGCCAG 
CAGCCACAGC TTCCTCTGAC CAGAATGCCT AGTGTCTCTC AGCCTGGAGT CCACACTGCC 
TGTCCTAGGC AGACTTTGGC CAATGGACCC TTTTCTGCAG GCCATGTTCC CTGTAGCACA 
TCAAGAACAC TGGGAAGTAC AGACACTGTT TTGATAGGCA ATAATCATGT AACAGGAAGT 
GGAAGTAATG GAAACGTGCC TTACCTGCAG CGAAACGCAC CCACTCTACC TCATAACCGC 
ACAAACCTGA CCAGCAGCAC AGAGGAGCCG TGGAAAAACC AACTATCTAA CTCCACTCAG 
GGGCTTCACA AAGGTCCGAG TTCACATTTG GCAGGTCCTA ATGGTGAACG ACCTCTATCT 
TCCACTGGGC CTTCCCAGCA TCTCCAGGCA GCTGGCTCTG GTATTCAGAA TCAGAATGGA 
CATCCCACCC TGCCTAGCAA TTCAGTAACA CAGGGGGCTG CTCTCAATCA CCTCTCCTCT 
CACACTGCTA CCTCAGGTGG ACAACAAGGC ATTACCTTAA CCAAAGAGAG CAAGCCTTCA 
GGAAACACAT TGACGGTGCC TGAAACAAGC AGGCAAACTG GAGAGACACC TAACAGCACT 
GCCAGTGTTG AGGGACTTCC TAATCATGTC CATCAGGTGA TGGCAGATGC TGTTTGCAGT 
CCTAGCCATG GAGATTCTAA GTCACCAGGT TTACTAAGTT CAGACAATCC TCAGCTCTCT 
GCCTTGTTGA TGGGAAAAGC TAATAACAAT GTGGGTCCTG GAACCTGTGA CAAAGTCAAT 
AACATCCACC CAACTGTCCA TACAAAGACT GATAATTCTG TTGCCTCTTC ACCATCTTCA 
GCCATTTCCA CAGCAACACC TTCTCCTAAG TCCACTGAAC AGACAACCAC AAACAGTGTT 
ACCAGCCTTA ACAGCCCTCA CAGTGGGCTG CACACAATTA ATGGAGAAGG AATGGAAGAA 
TCTCAGAGCC CCATTAAAAC AGATCTGCTT CTAGTTAGCC ACAGACCTAG TCCTCAGATC 
ATACCATCAA TGTCTGTGTC CATATATCCC AGCTCAGCAG AAGTTCTGAA AGCTTGCAGG 
AATCTAGGTA AAAACGGCCT GTCTAATAGT AGCATTCTGT TGGATAAATG TCCGCCTCCA 
AGACCACCAT CCTCACCATA CCCTCCCTTG CCAAAGGACA AGTTGAATCC ACCTACACCT 
AGTATTTATT TGGAAAATAA ACGTGATGCT TTCTTTCCTC CATTACATCA ATTTTGTACA 
AACCCAAACA ACCCTGTTAC AGTAATACGT GGCCTTGCTG GAGCTCTTAA ATTAGACTTG 
GGACTTTTCT CTACTAAAAC TTTGGTGGAA GCTAACAATG AACATATGGT AGAAGTGAGG 
ACACAGTTGT TACAACCAGC AGATGAAAAT TGGGACCCTA CTGGAACCAA GAAAATCTGG 
CACTGTGAAA GTAATAGATC TCATACTACA ATTGCTAAAT ATGCTCAGTA CCAGGCCTCC 
TCATTCCAAG AATCATTGAG AGAAGAAAAT GAGAAAAGAA GTCACCATAA AGACCACTCA 
GACAGTGAAT CTACATCATC AGATAATTCT GGGAAAAGAA GAAAAGGACC CTTTAAAACC 
ATTAAGTTTG GGACCAACAT TGACCTGTCT GATGACAAAA AGTGGAAGTT ACAGCTACAT 
GAGCTGACTA AACTTCCTGC CTTCGTGAGA GTTGTATCTG CAGGAAATCT TTTAAGCCAC 
GTTGGTCATA CTATACTGGG CATGAACACA GTTCAACTAT ACATGAAAGT TCCAGGAAGC 
AGAACACCAG GTCATCAAGA AAATAACAAC TTCTGTTCAG TTAATATAAA TATTGGCCCA 
GGTGACTGTG AATGGTTTGT TGTTCCTGAA GGCTACTGGG GTGTTTTGAA TGACTTCTGT 
GAAAAAAATA ATTTGAATTT CTTAATGGGT TCTTGGTGGC CCAACCTTGA AGATCTATAT 
GAAGCAAATG TTCCAGTGTA TAGGTTTATT CAGCGACCTG GAGATCTGGT CTGGATAAAT 
GCTGGCACTG TTCATTGGGT TCAAGCTATT GGCTGGTGCA ACAATATTGC TTGGAATGTT 
GGTCCACTTA CAGCCTGTCA GTATAAGTTA GCAGTGGAAC GTTATGAATG GAACAAGTTG 
CAAAATGTAA AGTCAATAGT ACCCATGGTT CATCTTTCCT GGAATATGGC ACGAAATATC 
AAGGTTTCAG ATCCAAAGCT TTTTGAAATG ATTAAGTATT GTCTTCTGAG AACGCTGAAG 
CAATGTCAGA CATTGAGGGA AGCTCTAATT GCTGCAGGAA AAGAGATCAT ATGGCACGGG 
CGGACAAAAG AAGAACCAGC TCATTATTGT AGTATTTGTG AGGTGGAGGT TTTTGATCTG 
CTTTTTGTCA CTAATGAGAG TAATTCTCGA AAAACCTACA TAGTACATTG CCAAGATTGT 
GCACGAAAAA CAAGTGGGAA TCTGGAAAAT TTTGTGGTGC TAGAACAGTA CAAAATGGAG 
GATCTGATGC AAGTCTATGA CCAATTTACA TTAGTAAGTG AAATCAACAT GCTCCTCCAT 
TACCATCCGC CTCATCTTGA TATTGTTCCA TGGACATTAA ACATGAGACC TTTTCTGCTA 
TTCAGAAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kdm6a ( NM_001310444.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001297373.1 Mus musculus lysine (K)-specific demethylase 6A (Kdm6a), transcript variant 2, mRNA.

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CloneID	OMu69025
Clone ID Related Accession (Same CDS sequence)	NM_001310444.1
Accession Version	NM_001310444.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4206bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	lysine-specific demethylase 6A isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL773547.6, AK164941.1, AJ002730.1, BC075703.1, AK164248.1 and AL732451.8. On Jul 14, 2015 this sequence version replaced XM_006527598.2. Transcript Variant: This variant (2) uses an alternate splice site in the penultimate exon, which results in a frameshift, compared to variant 1. It encodes isoform 2, which has a shorter and distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCCG CCGCCGCCGC CGCCGCTTTC 
GGTGATGAGG AAAAGAAAAT GGCGGCGGGA AAAGCGAGCG GCGAGAGCGA GGAGGCGTCC 
CCCAGCCTGA CAGCGGAGGA GAGGGAGGCG CTCGGCGGAC TGGACAGCCG CCTTTTCGGG 
TTCGTGAGGT TTCATGAAGA TGGCGCCAGG ATGAAGGCCC TGCTGGGCAA GGCTGTTCGC 
TGCTACGAAT CTCTAATCTT AAAAGCTGAA GGGAAAGTGG AGTCTGATTT CTTTTGTCAA 
TTAGGTCACT TCAACCTCTT ATTGGAAGAT TATCCAAAAG CATTATCTGC ATACCAGAGG 
TACTACAGTT TACAGTCTGA TTACTGGAAG AATGCTGCCT TTTTATATGG TCTTGGTTTG 
GTCTACTTCC ATTACAATGC ATTTCAGTGG GCTATTAAAG CATTTCAGGA GGTGCTTTAT 
GTCGATCCCA GCTTTTGTCG AGCCAAGGAA ATTCATTTAC GACTTGGGCT TATGTTCAAA 
GTGAACACAG ACTATGAGTC TAGTTTAAAG CATTTTCAGT TAGCTTTGGT TGACTGTAAT 
CCCTGCACTT TGTCCAATGC TGAAATTCAG TTTCACATTG CCCACTTATA TGAAACCCAG 
AGGAAGTATC ATTCTGCAAA AGAAGCTTAT GAGCAACTTT TGCAGACAGA AAACCTTTCT 
GCACAAGTAA AAGCAACTAT TTTACAACAA TTAGGTTGGA TGCATCACAC TGTGGATCTC 
CTGGGAGATA AGGCCACCAA GGAAAGTTAT GCTATTCAGT ATCTCCAGAA GTCCTTGGAA 
GCAGATCCAA ATTCTGGCCA GTCCTGGTAT TTCCTTGGAA GGTGCTATTC AAGTATTGGG 
AAAGTTCAGG ATGCCTTTAT ATCTTACAGG CAATCTATTG ATAAATCAGA AGCAAGTGCA 
GATACATGGT GTTCAATAGG TGTGCTCTAT CAACAGCAAA ATCAGCCTAT GGATGCTTTG 
CAAGCTTATA TTTGTGCTGT ACAATTGGAC CACGGTCATG CTGCAGCCTG GATGGATCTA 
GGCACTCTCT ATGAATCCTG CAACCAACCT CAGGATGCTA TTAAATGCTA TTTAAATGCA 
ACTAGAAGCA AAAATTGTAG TAATACCTCT GGACTTGCAG CACGAATTAA GTATTTACAG 
GCTCAGTTGT GTAACCTTCC ACAAGGTAGT CTACAGAATA AAACTAAATT ACTTCCTAGT 
ATTGAGGAGG CATGGAGCCT ACCAATCCCC GCAGAGCTTA CCTCCAGGCA GGGTGCCATG 
AACACAGCAC AGCAGAATAC TTCTGATAAT TGGAGTGGTG GCAATGCACC ACCTCCAGTA 
GAACAACAAA CTCATTCATG GTGTTTGACA CCACAGAAAT TACAGCACTT GGAACAGCTC 
CGAGCAAACA GAAATAATTT AAATCCAGCA CAGAAACTAA TGCTGGAACA GCTGGAAAGT 
CAGTTTGTCT TAATGCAGCA ACACCAAATG AGACAAACAG GAGTTGCACA GGTACGGCCT 
ACTGGAATTC TTAATGGGCC AACAGTTGAC TCATCACTGC CTACAAACTC AGTTTCTGGC 
CAGCAGCCAC AGCTTCCTCT GACCAGAATG CCTAGTGTCT CTCAGCCTGG AGTCCACACT 
GCCTGTCCTA GGCAGACTTT GGCCAATGGA CCCTTTTCTG CAGGCCATGT TCCCTGTAGC 
ACATCAAGAA CACTGGGAAG TACAGACACT GTTTTGATAG GCAATAATCA TGTAACAGGA 
AGTGGAAGTA ATGGAAACGT GCCTTACCTG CAGCGAAACG CACCCACTCT ACCTCATAAC 
CGCACAAACC TGACCAGCAG CACAGAGGAG CCGTGGAAAA ACCAACTATC TAACTCCACT 
CAGGGGCTTC ACAAAGGTCC GAGTTCACAT TTGGCAGGTC CTAATGGTGA ACGACCTCTA 
TCTTCCACTG GGCCTTCCCA GCATCTCCAG GCAGCTGGCT CTGGTATTCA GAATCAGAAT 
GGACATCCCA CCCTGCCTAG CAATTCAGTA ACACAGGGGG CTGCTCTCAA TCACCTCTCC 
TCTCACACTG CTACCTCAGG TGGACAACAA GGCATTACCT TAACCAAAGA GAGCAAGCCT 
TCAGGAAACA CATTGACGGT GCCTGAAACA AGCAGGCAAA CTGGAGAGAC ACCTAACAGC 
ACTGCCAGTG TTGAGGGACT TCCTAATCAT GTCCATCAGG TGATGGCAGA TGCTGTTTGC 
AGTCCTAGCC ATGGAGATTC TAAGTCACCA GGTTTACTAA GTTCAGACAA TCCTCAGCTC 
TCTGCCTTGT TGATGGGAAA AGCTAATAAC AATGTGGGTC CTGGAACCTG TGACAAAGTC 
AATAACATCC ACCCAACTGT CCATACAAAG ACTGATAATT CTGTTGCCTC TTCACCATCT 
TCAGCCATTT CCACAGCAAC ACCTTCTCCT AAGTCCACTG AACAGACAAC CACAAACAGT 
GTTACCAGCC TTAACAGCCC TCACAGTGGG CTGCACACAA TTAATGGAGA AGGAATGGAA 
GAATCTCAGA GCCCCATTAA AACAGATCTG CTTCTAGTTA GCCACAGACC TAGTCCTCAG 
ATCATACCAT CAATGTCTGT GTCCATATAT CCCAGCTCAG CAGAAGTTCT GAAAGCTTGC 
AGGAATCTAG GTAAAAACGG CCTGTCTAAT AGTAGCATTC TGTTGGATAA ATGTCCGCCT 
CCAAGACCAC CATCCTCACC ATACCCTCCC TTGCCAAAGG ACAAGTTGAA TCCACCTACA 
CCTAGTATTT ATTTGGAAAA TAAACGTGAT GCTTTCTTTC CTCCATTACA TCAATTTTGT 
ACAAACCCAA ACAACCCTGT TACAGTAATA CGTGGCCTTG CTGGAGCTCT TAAATTAGAC 
TTGGGACTTT TCTCTACTAA AACTTTGGTG GAAGCTAACA ATGAACATAT GGTAGAAGTG 
AGGACACAGT TGTTACAACC AGCAGATGAA AATTGGGACC CTACTGGAAC CAAGAAAATC 
TGGCACTGTG AAAGTAATAG ATCTCATACT ACAATTGCTA AATATGCTCA GTACCAGGCC 
TCCTCATTCC AAGAATCATT GAGAGAAGAA AATGAGAAAA GAAGTCACCA TAAAGACCAC 
TCAGACAGTG AATCTACATC ATCAGATAAT TCTGGGAAAA GAAGAAAAGG ACCCTTTAAA 
ACCATTAAGT TTGGGACCAA CATTGACCTG TCTGATGACA AAAAGTGGAA GTTACAGCTA 
CATGAGCTGA CTAAACTTCC TGCCTTCGTG AGAGTTGTAT CTGCAGGAAA TCTTTTAAGC 
CACGTTGGTC ATACTATACT GGGCATGAAC ACAGTTCAAC TATACATGAA AGTTCCAGGA 
AGCAGAACAC CAGGTCATCA AGAAAATAAC AACTTCTGTT CAGTTAATAT AAATATTGGC 
CCAGGTGACT GTGAATGGTT TGTTGTTCCT GAAGGCTACT GGGGTGTTTT GAATGACTTC 
TGTGAAAAAA ATAATTTGAA TTTCTTAATG GGTTCTTGGT GGCCCAACCT TGAAGATCTA 
TATGAAGCAA ATGTTCCAGT GTATAGGTTT ATTCAGCGAC CTGGAGATCT GGTCTGGATA 
AATGCTGGCA CTGTTCATTG GGTTCAAGCT ATTGGCTGGT GCAACAATAT TGCTTGGAAT 
GTTGGTCCAC TTACAGCCTG TCAGTATAAG TTAGCAGTGG AACGTTATGA ATGGAACAAG 
TTGCAAAATG TAAAGTCAAT AGTACCCATG GTTCATCTTT CCTGGAATAT GGCACGAAAT 
ATCAAGGTTT CAGATCCAAA GCTTTTTGAA ATGATTAAGT ATTGTCTTCT GAGAACGCTG 
AAGCAATGTC AGACATTGAG GGAAGCTCTA ATTGCTGCAG GAAAAGAGAT CATATGGCAC 
GGGCGGACAA AAGAAGAACC AGCTCATTAT TGTAGTATTT GTGAGGTGGA GGTTTTTGAT 
CTGCTTTTTG TCACTAATGA GAGTAATTCT CGAAAAACCT ACATAGTACA TTGCCAAGAT 
TGTGCACGAA AAACAAGTGG GAATCTGGAA AATTTTGTGG TGCTAGAACA GTACAAAATG 
GAGGATCTGA TGCAAGTCTA TGACCAATTT ACATTAGCTC CTCCATTACC ATCCGCCTCA 
TCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001297373.1
CDS	432..4637
Translation	MKSCGVSLATAAAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARMKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATILQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKNCSNTSGLAARIKYLQAQLCNLPQGSLQNKTKLLPSIEEAWSLPIPAELTSRQGAMNTAQQNTSDNWSGGNAPPPVEQQTHSWCLTPQKLQHLEQLRANRNNLNPAQKLMLEQLESQFVLMQQHQMRQTGVAQVRPTGILNGPTVDSSLPTNSVSGQQPQLPLTRMPSVSQPGVHTACPRQTLANGPFSAGHVPCSTSRTLGSTDTVLIGNNHVTGSGSNGNVPYLQRNAPTLPHNRTNLTSSTEEPWKNQLSNSTQGLHKGPSSHLAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNTLTVPETSRQTGETPNSTASVEGLPNHVHQVMADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGPGTCDKVNNIHPTVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPIKTDLLLVSHRPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGKRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQNVKSIVPMVHLSWNMARNIKVSDPKLFEMIKYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLAPPLPSASS

Target ORF information:

RefSeq Version	NM_001310444.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus lysine (K)-specific demethylase 6A (Kdm6a), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001310444.1

ORF Insert Sequence:

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCCG CCGCCGCCGC CGCCGCTTTC 
GGTGATGAGG AAAAGAAAAT GGCGGCGGGA AAAGCGAGCG GCGAGAGCGA GGAGGCGTCC 
CCCAGCCTGA CAGCGGAGGA GAGGGAGGCG CTCGGCGGAC TGGACAGCCG CCTTTTCGGG 
TTCGTGAGGT TTCATGAAGA TGGCGCCAGG ATGAAGGCCC TGCTGGGCAA GGCTGTTCGC 
TGCTACGAAT CTCTAATCTT AAAAGCTGAA GGGAAAGTGG AGTCTGATTT CTTTTGTCAA 
TTAGGTCACT TCAACCTCTT ATTGGAAGAT TATCCAAAAG CATTATCTGC ATACCAGAGG 
TACTACAGTT TACAGTCTGA TTACTGGAAG AATGCTGCCT TTTTATATGG TCTTGGTTTG 
GTCTACTTCC ATTACAATGC ATTTCAGTGG GCTATTAAAG CATTTCAGGA GGTGCTTTAT 
GTCGATCCCA GCTTTTGTCG AGCCAAGGAA ATTCATTTAC GACTTGGGCT TATGTTCAAA 
GTGAACACAG ACTATGAGTC TAGTTTAAAG CATTTTCAGT TAGCTTTGGT TGACTGTAAT 
CCCTGCACTT TGTCCAATGC TGAAATTCAG TTTCACATTG CCCACTTATA TGAAACCCAG 
AGGAAGTATC ATTCTGCAAA AGAAGCTTAT GAGCAACTTT TGCAGACAGA AAACCTTTCT 
GCACAAGTAA AAGCAACTAT TTTACAACAA TTAGGTTGGA TGCATCACAC TGTGGATCTC 
CTGGGAGATA AGGCCACCAA GGAAAGTTAT GCTATTCAGT ATCTCCAGAA GTCCTTGGAA 
GCAGATCCAA ATTCTGGCCA GTCCTGGTAT TTCCTTGGAA GGTGCTATTC AAGTATTGGG 
AAAGTTCAGG ATGCCTTTAT ATCTTACAGG CAATCTATTG ATAAATCAGA AGCAAGTGCA 
GATACATGGT GTTCAATAGG TGTGCTCTAT CAACAGCAAA ATCAGCCTAT GGATGCTTTG 
CAAGCTTATA TTTGTGCTGT ACAATTGGAC CACGGTCATG CTGCAGCCTG GATGGATCTA 
GGCACTCTCT ATGAATCCTG CAACCAACCT CAGGATGCTA TTAAATGCTA TTTAAATGCA 
ACTAGAAGCA AAAATTGTAG TAATACCTCT GGACTTGCAG CACGAATTAA GTATTTACAG 
GCTCAGTTGT GTAACCTTCC ACAAGGTAGT CTACAGAATA AAACTAAATT ACTTCCTAGT 
ATTGAGGAGG CATGGAGCCT ACCAATCCCC GCAGAGCTTA CCTCCAGGCA GGGTGCCATG 
AACACAGCAC AGCAGAATAC TTCTGATAAT TGGAGTGGTG GCAATGCACC ACCTCCAGTA 
GAACAACAAA CTCATTCATG GTGTTTGACA CCACAGAAAT TACAGCACTT GGAACAGCTC 
CGAGCAAACA GAAATAATTT AAATCCAGCA CAGAAACTAA TGCTGGAACA GCTGGAAAGT 
CAGTTTGTCT TAATGCAGCA ACACCAAATG AGACAAACAG GAGTTGCACA GGTACGGCCT 
ACTGGAATTC TTAATGGGCC AACAGTTGAC TCATCACTGC CTACAAACTC AGTTTCTGGC 
CAGCAGCCAC AGCTTCCTCT GACCAGAATG CCTAGTGTCT CTCAGCCTGG AGTCCACACT 
GCCTGTCCTA GGCAGACTTT GGCCAATGGA CCCTTTTCTG CAGGCCATGT TCCCTGTAGC 
ACATCAAGAA CACTGGGAAG TACAGACACT GTTTTGATAG GCAATAATCA TGTAACAGGA 
AGTGGAAGTA ATGGAAACGT GCCTTACCTG CAGCGAAACG CACCCACTCT ACCTCATAAC 
CGCACAAACC TGACCAGCAG CACAGAGGAG CCGTGGAAAA ACCAACTATC TAACTCCACT 
CAGGGGCTTC ACAAAGGTCC GAGTTCACAT TTGGCAGGTC CTAATGGTGA ACGACCTCTA 
TCTTCCACTG GGCCTTCCCA GCATCTCCAG GCAGCTGGCT CTGGTATTCA GAATCAGAAT 
GGACATCCCA CCCTGCCTAG CAATTCAGTA ACACAGGGGG CTGCTCTCAA TCACCTCTCC 
TCTCACACTG CTACCTCAGG TGGACAACAA GGCATTACCT TAACCAAAGA GAGCAAGCCT 
TCAGGAAACA CATTGACGGT GCCTGAAACA AGCAGGCAAA CTGGAGAGAC ACCTAACAGC 
ACTGCCAGTG TTGAGGGACT TCCTAATCAT GTCCATCAGG TGATGGCAGA TGCTGTTTGC 
AGTCCTAGCC ATGGAGATTC TAAGTCACCA GGTTTACTAA GTTCAGACAA TCCTCAGCTC 
TCTGCCTTGT TGATGGGAAA AGCTAATAAC AATGTGGGTC CTGGAACCTG TGACAAAGTC 
AATAACATCC ACCCAACTGT CCATACAAAG ACTGATAATT CTGTTGCCTC TTCACCATCT 
TCAGCCATTT CCACAGCAAC ACCTTCTCCT AAGTCCACTG AACAGACAAC CACAAACAGT 
GTTACCAGCC TTAACAGCCC TCACAGTGGG CTGCACACAA TTAATGGAGA AGGAATGGAA 
GAATCTCAGA GCCCCATTAA AACAGATCTG CTTCTAGTTA GCCACAGACC TAGTCCTCAG 
ATCATACCAT CAATGTCTGT GTCCATATAT CCCAGCTCAG CAGAAGTTCT GAAAGCTTGC 
AGGAATCTAG GTAAAAACGG CCTGTCTAAT AGTAGCATTC TGTTGGATAA ATGTCCGCCT 
CCAAGACCAC CATCCTCACC ATACCCTCCC TTGCCAAAGG ACAAGTTGAA TCCACCTACA 
CCTAGTATTT ATTTGGAAAA TAAACGTGAT GCTTTCTTTC CTCCATTACA TCAATTTTGT 
ACAAACCCAA ACAACCCTGT TACAGTAATA CGTGGCCTTG CTGGAGCTCT TAAATTAGAC 
TTGGGACTTT TCTCTACTAA AACTTTGGTG GAAGCTAACA ATGAACATAT GGTAGAAGTG 
AGGACACAGT TGTTACAACC AGCAGATGAA AATTGGGACC CTACTGGAAC CAAGAAAATC 
TGGCACTGTG AAAGTAATAG ATCTCATACT ACAATTGCTA AATATGCTCA GTACCAGGCC 
TCCTCATTCC AAGAATCATT GAGAGAAGAA AATGAGAAAA GAAGTCACCA TAAAGACCAC 
TCAGACAGTG AATCTACATC ATCAGATAAT TCTGGGAAAA GAAGAAAAGG ACCCTTTAAA 
ACCATTAAGT TTGGGACCAA CATTGACCTG TCTGATGACA AAAAGTGGAA GTTACAGCTA 
CATGAGCTGA CTAAACTTCC TGCCTTCGTG AGAGTTGTAT CTGCAGGAAA TCTTTTAAGC 
CACGTTGGTC ATACTATACT GGGCATGAAC ACAGTTCAAC TATACATGAA AGTTCCAGGA 
AGCAGAACAC CAGGTCATCA AGAAAATAAC AACTTCTGTT CAGTTAATAT AAATATTGGC 
CCAGGTGACT GTGAATGGTT TGTTGTTCCT GAAGGCTACT GGGGTGTTTT GAATGACTTC 
TGTGAAAAAA ATAATTTGAA TTTCTTAATG GGTTCTTGGT GGCCCAACCT TGAAGATCTA 
TATGAAGCAA ATGTTCCAGT GTATAGGTTT ATTCAGCGAC CTGGAGATCT GGTCTGGATA 
AATGCTGGCA CTGTTCATTG GGTTCAAGCT ATTGGCTGGT GCAACAATAT TGCTTGGAAT 
GTTGGTCCAC TTACAGCCTG TCAGTATAAG TTAGCAGTGG AACGTTATGA ATGGAACAAG 
TTGCAAAATG TAAAGTCAAT AGTACCCATG GTTCATCTTT CCTGGAATAT GGCACGAAAT 
ATCAAGGTTT CAGATCCAAA GCTTTTTGAA ATGATTAAGT ATTGTCTTCT GAGAACGCTG 
AAGCAATGTC AGACATTGAG GGAAGCTCTA ATTGCTGCAG GAAAAGAGAT CATATGGCAC 
GGGCGGACAA AAGAAGAACC AGCTCATTAT TGTAGTATTT GTGAGGTGGA GGTTTTTGAT 
CTGCTTTTTG TCACTAATGA GAGTAATTCT CGAAAAACCT ACATAGTACA TTGCCAAGAT 
TGTGCACGAA AAACAAGTGG GAATCTGGAA AATTTTGTGG TGCTAGAACA GTACAAAATG 
GAGGATCTGA TGCAAGTCTA TGACCAATTT ACATTAGCTC CTCCATTACC ATCCGCCTCA 
TCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kdm6a ( XM_006527594.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527657.1 Mus musculus lysine (K)-specific demethylase 6A (Kdm6a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu55891
Clone ID Related Accession (Same CDS sequence)	XM_006527594.4 , XM_006527594.3
Accession Version	XM_006527594.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	lysine-specific demethylase 6A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006527594.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCCG CCGCCGCCGC CGCCGCTTTC 
GGTGATGAGG AAAAGAAAAT GGCGGCGGGA AAAGCGAGCG GCGAGAGCGA GGAGGCGTCC 
CCCAGCCTGA CAGCGGAGGA GAGGGAGGCG CTCGGCGGAC TGGACAGCCG CCTTTTCGGG 
TTCGTGAGGT TTCATGAAGA TGGCGCCAGG ATGAAGGCCC TGCTGGGCAA GGCTGTTCGC 
TGCTACGAAT CTCTAATCTT AAAAGCTGAA GGGAAAGTGG AGTCTGATTT CTTTTGTCAA 
TTAGGTCACT TCAACCTCTT ATTGGAAGAT TATCCAAAAG CATTATCTGC ATACCAGAGG 
TACTACAGTT TACAGTCTGA TTACTGGAAG AATGCTGCCT TTTTATATGG TCTTGGTTTG 
GTCTACTTCC ATTACAATGC ATTTCAGTGG GCTATTAAAG CATTTCAGGA GGTGCTTTAT 
GTCGATCCCA GCTTTTGTCG AGCCAAGGAA ATTCATTTAC GACTTGGGCT TATGTTCAAA 
GTGAACACAG ACTATGAGTC TAGTTTAAAG CATTTTCAGT TAGCTTTGGT TGACTGTAAT 
CCCTGCACTT TGTCCAATGC TGAAATTCAG TTTCACATTG CCCACTTATA TGAAACCCAG 
AGGAAGTATC ATTCTGCAAA AGAAGCTTAT GAGCAACTTT TGCAGACAGA AAACCTTTCT 
GCACAAGTAA AAGCAACTAT TTTACAACAA TTAGGTTGGA TGCATCACAC TGTGGATCTC 
CTGGGAGATA AGGCCACCAA GGAAAGTTAT GCTATTCAGT ATCTCCAGAA GTCCTTGGAA 
GCAGATCCAA ATTCTGGCCA GTCCTGGTAT TTCCTTGGAA GGTGCTATTC AAGTATTGGG 
AAAGTTCAGG ATGCCTTTAT ATCTTACAGG CAATCTATTG ATAAATCAGA AGCAAGTGCA 
GATACATGGT GTTCAATAGG TGTGCTCTAT CAACAGCAAA ATCAGCCTAT GGATGCTTTG 
CAAGCTTATA TTTGTGCTGT ACAATTGGAC CACGGTCATG CTGCAGCCTG GATGGATCTA 
GGCACTCTCT ATGAATCCTG CAACCAACCT CAGGATGCTA TTAAATGCTA TTTAAATGCA 
ACTAGAAGCA AAAATTGTAG TAATACCTCT GGACTTGCAG CACGAATTAA GTATTTACAG 
GCTCAGTTGT GTAACCTTCC ACAAGGTAGT CTACAGAATA AAACTAAATT ACTTCCTAGT 
ATTGAGGAGG CATGGAGCCT ACCAATCCCC GCAGAGCTTA CCTCCAGGCA GGGTGCCATG 
AACACAGCAC AGCAGGCATG TAAACCTCAT CATCCAAATA CTGAACCTGT ATTAGGCCTC 
AGTCAAACAC CAATTTCACA GCAGTCCTTG CCACTACACA TGATTCCTCC TAGCCAAGTA 
GATGACCTGT CCAGTCCTGC CAAGAGGAAA AGAACATCTA GTCCAACAAA GAATACTTCT 
GATAATTGGA GTGGTGGCAA TGCACCACCT CCAGTAGAAC AACAAACTCA TTCATGGTGT 
TTGACACCAC AGAAATTACA GCACTTGGAA CAGCTCCGAG CAAACAGAAA TAATTTAAAT 
CCAGCACAGA AACTAATGCT GGAACAGCTG GAAAGTCAGT TTGTCTTAAT GCAGCAACAC 
CAAATGAGAC AAACAGGAGT TGCACAGGTA CGGCCTACTG GAATTCTTAA TGGGCCAACA 
GTTGACTCAT CACTGCCTAC AAACTCAGTT TCTGGCCAGC AGCCACAGCT TCCTCTGACC 
AGAATGCCTA GTGTCTCTCA GCCTGGAGTC CACACTGCCT GTCCTAGGCA GACTTTGGCC 
AATGGACCCT TTTCTGCAGG CCATGTTCCC TGTAGCACAT CAAGAACACT GGGAAGTACA 
GACACTGTTT TGATAGGCAA TAATCATGTA ACAGGAAGTG GAAGTAATGG AAACGTGCCT 
TACCTGCAGC GAAACGCACC CACTCTACCT CATAACCGCA CAAACCTGAC CAGCAGCACA 
GAGGAGCCGT GGAAAAACCA ACTATCTAAC TCCACTCAGG GGCTTCACAA AGGTCCGAGT 
TCACATTTGG CAGGTCCTAA TGGTGAACGA CCTCTATCTT CCACTGGGCC TTCCCAGCAT 
CTCCAGGCAG CTGGCTCTGG TATTCAGAAT CAGAATGGAC ATCCCACCCT GCCTAGCAAT 
TCAGTAACAC AGGGGGCTGC TCTCAATCAC CTCTCCTCTC ACACTGCTAC CTCAGGTGGA 
CAACAAGGCA TTACCTTAAC CAAAGAGAGC AAGCCTTCAG GAAACACATT GACGGTGCCT 
GAAACAAGCA GGCAAACTGG AGAGACACCT AACAGCACTG CCAGTGTTGA GGGACTTCCT 
AATCATGTCC ATCAGGTGAT GGCAGATGCT GTTTGCAGTC CTAGCCATGG AGATTCTAAG 
TCACCAGGTT TACTAAGTTC AGACAATCCT CAGCTCTCTG CCTTGTTGAT GGGAAAAGCT 
AATAACAATG TGGGTCCTGG AACCTGTGAC AAAGTCAATA ACATCCACCC AACTGTCCAT 
ACAAAGACTG ATAATTCTGT TGCCTCTTCA CCATCTTCAG CCATTTCCAC AGCAACACCT 
TCTCCTAAGT CCACTGAACA GACAACCACA AACAGTGTTA CCAGCCTTAA CAGCCCTCAC 
AGTGGGCTGC ACACAATTAA TGGAGAAGGA ATGGAAGAAT CTCAGAGCCC CATTAAAACA 
GATCTGCTTC TAGTTAGCCA CAGACCTAGT CCTCAGATCA TACCATCAAT GTCTGTGTCC 
ATATATCCCA GCTCAGCAGA AGTTCTGAAA GCTTGCAGGA ATCTAGGTAA AAACGGCCTG 
TCTAATAGTA GCATTCTGTT GGATAAATGT CCGCCTCCAA GACCACCATC CTCACCATAC 
CCTCCCTTGC CAAAGGACAA GTTGAATCCA CCTACACCTA GTATTTATTT GGAAAATAAA 
CGTGATGCTT TCTTTCCTCC ATTACATCAA TTTTGTACAA ACCCAAACAA CCCTGTTACA 
GTAATACGTG GCCTTGCTGG AGCTCTTAAA TTAGACTTGG GACTTTTCTC TACTAAAACT 
TTGGTGGAAG CTAACAATGA ACATATGGTA GAAGTGAGGA CACAGTTGTT ACAACCAGCA 
GATGAAAATT GGGACCCTAC TGGAACCAAG AAAATCTGGC ACTGTGAAAG TAATAGATCT 
CATACTACAA TTGCTAAATA TGCTCAGTAC CAGGCCTCCT CATTCCAAGA ATCATTGAGA 
GAAGAAAATG AGAAAAGAAG TCACCATAAA GACCACTCAG ACAGTGAATC TACATCATCA 
GATAATTCTG GGAAAAGAAG AAAAGGACCC TTTAAAACCA TTAAGTTTGG GACCAACATT 
GACCTGTCTG ATGACAAAAA GTGGAAGTTA CAGCTACATG AGCTGACTAA ACTTCCTGCC 
TTCGTGAGAG TTGTATCTGC AGGAAATCTT TTAAGCCACG TTGGTCATAC TATACTGGGC 
ATGAACACAG TTCAACTATA CATGAAAGTT CCAGGAAGCA GAACACCAGG TCATCAAGAA 
AATAACAACT TCTGTTCAGT TAATATAAAT ATTGGCCCAG GTGACTGTGA ATGGTTTGTT 
GTTCCTGAAG GCTACTGGGG TGTTTTGAAT GACTTCTGTG AAAAAAATAA TTTGAATTTC 
TTAATGGGTT CTTGGTGGCC CAACCTTGAA GATCTATATG AAGCAAATGT TCCAGTGTAT 
AGGTTTATTC AGCGACCTGG AGATCTGGTC TGGATAAATG CTGGCACTGT TCATTGGGTT 
CAAGCTATTG GCTGGTGCAA CAATATTGCT TGGAATGTTG GTCCACTTAC AGCCTGTCAG 
TATAAGTTAG CAGTGGAACG TTATGAATGG AACAAGTTGC AAAATGTAAA GTCAATAGTA 
CCCATGGTTC ATCTTTCCTG GAATATGGCA CGAAATATCA AGGTTTCAGA TCCAAAGCTT 
TTTGAAATGA TTAAGTATTG TCTTCTGAGA ACGCTGAAGC AATGTCAGAC ATTGAGGGAA 
GCTCTAATTG CTGCAGGAAA AGAGATCATA TGGCACGGGC GGACAAAAGA AGAACCAGCT 
CATTATTGTA GTATTTGTGA GGTGGAGGTT TTTGATCTGC TTTTTGTCAC TAATGAGAGT 
AATTCTCGAA AAACCTACAT AGTACATTGC CAAGATTGTG CACGAAAAAC AAGTGGGAAT 
CTGGAAAATT TTGTGGTGCT AGAACAGTAC AAAATGGAGG ATCTGATGCA AGTCTATGAC 
CAATTTACAT TAGTAAGTGA AATCAACATG CTCCTCCATT ACCATCCGCC TCATCTTGAT 
ATTGTTCCAT GGACATTAAA CATGAGACCT TTTCTGCTAT TCAGAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006527657.1
CDS	643..5073
Translation	MKSCGVSLATAAAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARMKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATILQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKNCSNTSGLAARIKYLQAQLCNLPQGSLQNKTKLLPSIEEAWSLPIPAELTSRQGAMNTAQQACKPHHPNTEPVLGLSQTPISQQSLPLHMIPPSQVDDLSSPAKRKRTSSPTKNTSDNWSGGNAPPPVEQQTHSWCLTPQKLQHLEQLRANRNNLNPAQKLMLEQLESQFVLMQQHQMRQTGVAQVRPTGILNGPTVDSSLPTNSVSGQQPQLPLTRMPSVSQPGVHTACPRQTLANGPFSAGHVPCSTSRTLGSTDTVLIGNNHVTGSGSNGNVPYLQRNAPTLPHNRTNLTSSTEEPWKNQLSNSTQGLHKGPSSHLAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNTLTVPETSRQTGETPNSTASVEGLPNHVHQVMADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGPGTCDKVNNIHPTVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPIKTDLLLVSHRPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGKRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQNVKSIVPMVHLSWNMARNIKVSDPKLFEMIKYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLVSEINMLLHYHPPHLDIVPWTLNMRPFLLFRK

Target ORF information:

RefSeq Version	XM_006527594.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus lysine (K)-specific demethylase 6A (Kdm6a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527594.3

ORF Insert Sequence:

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCCG CCGCCGCCGC CGCCGCTTTC 
GGTGATGAGG AAAAGAAAAT GGCGGCGGGA AAAGCGAGCG GCGAGAGCGA GGAGGCGTCC 
CCCAGCCTGA CAGCGGAGGA GAGGGAGGCG CTCGGCGGAC TGGACAGCCG CCTTTTCGGG 
TTCGTGAGGT TTCATGAAGA TGGCGCCAGG ATGAAGGCCC TGCTGGGCAA GGCTGTTCGC 
TGCTACGAAT CTCTAATCTT AAAAGCTGAA GGGAAAGTGG AGTCTGATTT CTTTTGTCAA 
TTAGGTCACT TCAACCTCTT ATTGGAAGAT TATCCAAAAG CATTATCTGC ATACCAGAGG 
TACTACAGTT TACAGTCTGA TTACTGGAAG AATGCTGCCT TTTTATATGG TCTTGGTTTG 
GTCTACTTCC ATTACAATGC ATTTCAGTGG GCTATTAAAG CATTTCAGGA GGTGCTTTAT 
GTCGATCCCA GCTTTTGTCG AGCCAAGGAA ATTCATTTAC GACTTGGGCT TATGTTCAAA 
GTGAACACAG ACTATGAGTC TAGTTTAAAG CATTTTCAGT TAGCTTTGGT TGACTGTAAT 
CCCTGCACTT TGTCCAATGC TGAAATTCAG TTTCACATTG CCCACTTATA TGAAACCCAG 
AGGAAGTATC ATTCTGCAAA AGAAGCTTAT GAGCAACTTT TGCAGACAGA AAACCTTTCT 
GCACAAGTAA AAGCAACTAT TTTACAACAA TTAGGTTGGA TGCATCACAC TGTGGATCTC 
CTGGGAGATA AGGCCACCAA GGAAAGTTAT GCTATTCAGT ATCTCCAGAA GTCCTTGGAA 
GCAGATCCAA ATTCTGGCCA GTCCTGGTAT TTCCTTGGAA GGTGCTATTC AAGTATTGGG 
AAAGTTCAGG ATGCCTTTAT ATCTTACAGG CAATCTATTG ATAAATCAGA AGCAAGTGCA 
GATACATGGT GTTCAATAGG TGTGCTCTAT CAACAGCAAA ATCAGCCTAT GGATGCTTTG 
CAAGCTTATA TTTGTGCTGT ACAATTGGAC CACGGTCATG CTGCAGCCTG GATGGATCTA 
GGCACTCTCT ATGAATCCTG CAACCAACCT CAGGATGCTA TTAAATGCTA TTTAAATGCA 
ACTAGAAGCA AAAATTGTAG TAATACCTCT GGACTTGCAG CACGAATTAA GTATTTACAG 
GCTCAGTTGT GTAACCTTCC ACAAGGTAGT CTACAGAATA AAACTAAATT ACTTCCTAGT 
ATTGAGGAGG CATGGAGCCT ACCAATCCCC GCAGAGCTTA CCTCCAGGCA GGGTGCCATG 
AACACAGCAC AGCAGGCATG TAAACCTCAT CATCCAAATA CTGAACCTGT ATTAGGCCTC 
AGTCAAACAC CAATTTCACA GCAGTCCTTG CCACTACACA TGATTCCTCC TAGCCAAGTA 
GATGACCTGT CCAGTCCTGC CAAGAGGAAA AGAACATCTA GTCCAACAAA GAATACTTCT 
GATAATTGGA GTGGTGGCAA TGCACCACCT CCAGTAGAAC AACAAACTCA TTCATGGTGT 
TTGACACCAC AGAAATTACA GCACTTGGAA CAGCTCCGAG CAAACAGAAA TAATTTAAAT 
CCAGCACAGA AACTAATGCT GGAACAGCTG GAAAGTCAGT TTGTCTTAAT GCAGCAACAC 
CAAATGAGAC AAACAGGAGT TGCACAGGTA CGGCCTACTG GAATTCTTAA TGGGCCAACA 
GTTGACTCAT CACTGCCTAC AAACTCAGTT TCTGGCCAGC AGCCACAGCT TCCTCTGACC 
AGAATGCCTA GTGTCTCTCA GCCTGGAGTC CACACTGCCT GTCCTAGGCA GACTTTGGCC 
AATGGACCCT TTTCTGCAGG CCATGTTCCC TGTAGCACAT CAAGAACACT GGGAAGTACA 
GACACTGTTT TGATAGGCAA TAATCATGTA ACAGGAAGTG GAAGTAATGG AAACGTGCCT 
TACCTGCAGC GAAACGCACC CACTCTACCT CATAACCGCA CAAACCTGAC CAGCAGCACA 
GAGGAGCCGT GGAAAAACCA ACTATCTAAC TCCACTCAGG GGCTTCACAA AGGTCCGAGT 
TCACATTTGG CAGGTCCTAA TGGTGAACGA CCTCTATCTT CCACTGGGCC TTCCCAGCAT 
CTCCAGGCAG CTGGCTCTGG TATTCAGAAT CAGAATGGAC ATCCCACCCT GCCTAGCAAT 
TCAGTAACAC AGGGGGCTGC TCTCAATCAC CTCTCCTCTC ACACTGCTAC CTCAGGTGGA 
CAACAAGGCA TTACCTTAAC CAAAGAGAGC AAGCCTTCAG GAAACACATT GACGGTGCCT 
GAAACAAGCA GGCAAACTGG AGAGACACCT AACAGCACTG CCAGTGTTGA GGGACTTCCT 
AATCATGTCC ATCAGGTGAT GGCAGATGCT GTTTGCAGTC CTAGCCATGG AGATTCTAAG 
TCACCAGGTT TACTAAGTTC AGACAATCCT CAGCTCTCTG CCTTGTTGAT GGGAAAAGCT 
AATAACAATG TGGGTCCTGG AACCTGTGAC AAAGTCAATA ACATCCACCC AACTGTCCAT 
ACAAAGACTG ATAATTCTGT TGCCTCTTCA CCATCTTCAG CCATTTCCAC AGCAACACCT 
TCTCCTAAGT CCACTGAACA GACAACCACA AACAGTGTTA CCAGCCTTAA CAGCCCTCAC 
AGTGGGCTGC ACACAATTAA TGGAGAAGGA ATGGAAGAAT CTCAGAGCCC CATTAAAACA 
GATCTGCTTC TAGTTAGCCA CAGACCTAGT CCTCAGATCA TACCATCAAT GTCTGTGTCC 
ATATATCCCA GCTCAGCAGA AGTTCTGAAA GCTTGCAGGA ATCTAGGTAA AAACGGCCTG 
TCTAATAGTA GCATTCTGTT GGATAAATGT CCGCCTCCAA GACCACCATC CTCACCATAC 
CCTCCCTTGC CAAAGGACAA GTTGAATCCA CCTACACCTA GTATTTATTT GGAAAATAAA 
CGTGATGCTT TCTTTCCTCC ATTACATCAA TTTTGTACAA ACCCAAACAA CCCTGTTACA 
GTAATACGTG GCCTTGCTGG AGCTCTTAAA TTAGACTTGG GACTTTTCTC TACTAAAACT 
TTGGTGGAAG CTAACAATGA ACATATGGTA GAAGTGAGGA CACAGTTGTT ACAACCAGCA 
GATGAAAATT GGGACCCTAC TGGAACCAAG AAAATCTGGC ACTGTGAAAG TAATAGATCT 
CATACTACAA TTGCTAAATA TGCTCAGTAC CAGGCCTCCT CATTCCAAGA ATCATTGAGA 
GAAGAAAATG AGAAAAGAAG TCACCATAAA GACCACTCAG ACAGTGAATC TACATCATCA 
GATAATTCTG GGAAAAGAAG AAAAGGACCC TTTAAAACCA TTAAGTTTGG GACCAACATT 
GACCTGTCTG ATGACAAAAA GTGGAAGTTA CAGCTACATG AGCTGACTAA ACTTCCTGCC 
TTCGTGAGAG TTGTATCTGC AGGAAATCTT TTAAGCCACG TTGGTCATAC TATACTGGGC 
ATGAACACAG TTCAACTATA CATGAAAGTT CCAGGAAGCA GAACACCAGG TCATCAAGAA 
AATAACAACT TCTGTTCAGT TAATATAAAT ATTGGCCCAG GTGACTGTGA ATGGTTTGTT 
GTTCCTGAAG GCTACTGGGG TGTTTTGAAT GACTTCTGTG AAAAAAATAA TTTGAATTTC 
TTAATGGGTT CTTGGTGGCC CAACCTTGAA GATCTATATG AAGCAAATGT TCCAGTGTAT 
AGGTTTATTC AGCGACCTGG AGATCTGGTC TGGATAAATG CTGGCACTGT TCATTGGGTT 
CAAGCTATTG GCTGGTGCAA CAATATTGCT TGGAATGTTG GTCCACTTAC AGCCTGTCAG 
TATAAGTTAG CAGTGGAACG TTATGAATGG AACAAGTTGC AAAATGTAAA GTCAATAGTA 
CCCATGGTTC ATCTTTCCTG GAATATGGCA CGAAATATCA AGGTTTCAGA TCCAAAGCTT 
TTTGAAATGA TTAAGTATTG TCTTCTGAGA ACGCTGAAGC AATGTCAGAC ATTGAGGGAA 
GCTCTAATTG CTGCAGGAAA AGAGATCATA TGGCACGGGC GGACAAAAGA AGAACCAGCT 
CATTATTGTA GTATTTGTGA GGTGGAGGTT TTTGATCTGC TTTTTGTCAC TAATGAGAGT 
AATTCTCGAA AAACCTACAT AGTACATTGC CAAGATTGTG CACGAAAAAC AAGTGGGAAT 
CTGGAAAATT TTGTGGTGCT AGAACAGTAC AAAATGGAGG ATCTGATGCA AGTCTATGAC 
CAATTTACAT TAGTAAGTGA AATCAACATG CTCCTCCATT ACCATCCGCC TCATCTTGAT 
ATTGTTCCAT GGACATTAAA CATGAGACCT TTTCTGCTAT TCAGAAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kdm6a ( XM_006527595.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527658.1 Mus musculus lysine (K)-specific demethylase 6A (Kdm6a), transcript variant X2, mRNA.

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CloneID	OMu55892
Clone ID Related Accession (Same CDS sequence)	XM_006527595.4 , XM_006527595.3
Accession Version	XM_006527595.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4362bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	lysine-specific demethylase 6A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006527595.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCCG CCGCCGCCGC CGCCGCTTTC 
GGTGATGAGG AAAAGAAAAT GGCGGCGGGA AAAGCGAGCG GCGAGAGCGA GGAGGCGTCC 
CCCAGCCTGA CAGCGGAGGA GAGGGAGGCG CTCGGCGGAC TGGACAGCCG CCTTTTCGGG 
TTCGTGAGGT TTCATGAAGA TGGCGCCAGG ATGAAGGCCC TGCTGGGCAA GGCTGTTCGC 
TGCTACGAAT CTCTAATCTT AAAAGCTGAA GGGAAAGTGG AGTCTGATTT CTTTTGTCAA 
TTAGGTCACT TCAACCTCTT ATTGGAAGAT TATCCAAAAG CATTATCTGC ATACCAGAGG 
TACTACAGTT TACAGTCTGA TTACTGGAAG AATGCTGCCT TTTTATATGG TCTTGGTTTG 
GTCTACTTCC ATTACAATGC ATTTCAGTGG GCTATTAAAG CATTTCAGGA GGTGCTTTAT 
GTCGATCCCA GCTTTTGTCG AGCCAAGGAA ATTCATTTAC GACTTGGGCT TATGTTCAAA 
GTGAACACAG ACTATGAGTC TAGTTTAAAG CATTTTCAGT TAGCTTTGGT TGACTGTAAT 
CCCTGCACTT TGTCCAATGC TGAAATTCAG TTTCACATTG CCCACTTATA TGAAACCCAG 
AGGAAGTATC ATTCTGCAAA AGAAGCTTAT GAGCAACTTT TGCAGACAGA AAACCTTTCT 
GCACAAGTAA AAGCAACTAT TTTACAACAA TTAGGTTGGA TGCATCACAC TGTGGATCTC 
CTGGGAGATA AGGCCACCAA GGAAAGTTAT GCTATTCAGT ATCTCCAGAA GTCCTTGGAA 
GCAGATCCAA ATTCTGGCCA GTCCTGGTAT TTCCTTGGAA GGTGCTATTC AAGTATTGGG 
AAAGTTCAGG ATGCCTTTAT ATCTTACAGG CAATCTATTG ATAAATCAGA AGCAAGTGCA 
GATACATGGT GTTCAATAGG TGTGCTCTAT CAACAGCAAA ATCAGCCTAT GGATGCTTTG 
CAAGCTTATA TTTGTGCTGT ACAATTGGAC CACGGTCATG CTGCAGCCTG GATGGATCTA 
GGCACTCTCT ATGAATCCTG CAACCAACCT CAGGATGCTA TTAAATGCTA TTTAAATGCA 
ACTAGAAGCA AAAATTGTAG TAATACCTCT GGACTTGCAG CACGAATTAA GTATTTACAG 
GCTCAGTTGT GTAACCTTCC ACAAGGTAGT CTACAGAATA AAACTAAATT ACTTCCTAGT 
ATTGAGGAGG CATGGAGCCT ACCAATCCCC GCAGAGCTTA CCTCCAGGCA GGGTGCCATG 
AACACAGCAC AGCAGGCATG TAAACCTCAT CATCCAAATA CTGAACCTGT ATTAGGCCTC 
AGTCAAACAC CAATTTCACA GCAGTCCTTG CCACTACACA TGATTCCTCC TAGCCAAGTA 
GATGACCTGT CCAGTCCTGC CAAGAGGAAA AGAACATCTA GTCCAACAAA GAATACTTCT 
GATAATTGGA GTGGTGGCAA TGCACCACCT CCAGTAGAAC AACAAACTCA TTCATGGTGT 
TTGACACCAC AGAAATTACA GCACTTGGAA CAGCTCCGAG CAAACAGAAA TAATTTAAAT 
CCAGCACAGA AACTAATGCT GGAACAGCTG GAAAGTCAGT TTGTCTTAAT GCAGCAACAC 
CAAATGAGAC AAACAGGAGT TGCACAGGTA CGGCCTACTG GAATTCTTAA TGGGCCAACA 
GTTGACTCAT CACTGCCTAC AAACTCAGTT TCTGGCCAGC AGCCACAGCT TCCTCTGACC 
AGAATGCCTA GTGTCTCTCA GCCTGGAGTC CACACTGCCT GTCCTAGGCA GACTTTGGCC 
AATGGACCCT TTTCTGCAGG CCATGTTCCC TGTAGCACAT CAAGAACACT GGGAAGTACA 
GACACTGTTT TGATAGGCAA TAATCATGTA ACAGGAAGTG GAAGTAATGG AAACGTGCCT 
TACCTGCAGC GAAACGCACC CACTCTACCT CATAACCGCA CAAACCTGAC CAGCAGCACA 
GAGGAGCCGT GGAAAAACCA ACTATCTAAC TCCACTCAGG GGCTTCACAA AGGTCCGAGT 
TCACATTTGG CAGGTCCTAA TGGTGAACGA CCTCTATCTT CCACTGGGCC TTCCCAGCAT 
CTCCAGGCAG CTGGCTCTGG TATTCAGAAT CAGAATGGAC ATCCCACCCT GCCTAGCAAT 
TCAGTAACAC AGGGGGCTGC TCTCAATCAC CTCTCCTCTC ACACTGCTAC CTCAGGTGGA 
CAACAAGGCA TTACCTTAAC CAAAGAGAGC AAGCCTTCAG GAAACACATT GACGGTGCCT 
GAAACAAGCA GGCAAACTGG AGAGACACCT AACAGCACTG CCAGTGTTGA GGGACTTCCT 
AATCATGTCC ATCAGGTGAT GGCAGATGCT GTTTGCAGTC CTAGCCATGG AGATTCTAAG 
TCACCAGGTT TACTAAGTTC AGACAATCCT CAGCTCTCTG CCTTGTTGAT GGGAAAAGCT 
AATAACAATG TGGGTCCTGG AACCTGTGAC AAAGTCAATA ACATCCACCC AACTGTCCAT 
ACAAAGACTG ATAATTCTGT TGCCTCTTCA CCATCTTCAG CCATTTCCAC AGCAACACCT 
TCTCCTAAGT CCACTGAACA GACAACCACA AACAGTGTTA CCAGCCTTAA CAGCCCTCAC 
AGTGGGCTGC ACACAATTAA TGGAGAAGGA ATGGAAGAAT CTCAGAGCCC CATTAAAACA 
GATCTGCTTC TAGTTAGCCA CAGACCTAGT CCTCAGATCA TACCATCAAT GTCTGTGTCC 
ATATATCCCA GCTCAGCAGA AGTTCTGAAA GCTTGCAGGA ATCTAGGTAA AAACGGCCTG 
TCTAATAGTA GCATTCTGTT GGATAAATGT CCGCCTCCAA GACCACCATC CTCACCATAC 
CCTCCCTTGC CAAAGGACAA GTTGAATCCA CCTACACCTA GTATTTATTT GGAAAATAAA 
CGTGATGCTT TCTTTCCTCC ATTACATCAA TTTTGTACAA ACCCAAACAA CCCTGTTACA 
GTAATACGTG GCCTTGCTGG AGCTCTTAAA TTAGACTTGG GACTTTTCTC TACTAAAACT 
TTGGTGGAAG CTAACAATGA ACATATGGTA GAAGTGAGGA CACAGTTGTT ACAACCAGCA 
GATGAAAATT GGGACCCTAC TGGAACCAAG AAAATCTGGC ACTGTGAAAG TAATAGATCT 
CATACTACAA TTGCTAAATA TGCTCAGTAC CAGGCCTCCT CATTCCAAGA ATCATTGAGA 
GAAGAAAATG AGAAAAGAAG TCACCATAAA GACCACTCAG ACAGTGAATC TACATCATCA 
GATAATTCTG GGAAAAGAAG AAAAGGACCC TTTAAAACCA TTAAGTTTGG GACCAACATT 
GACCTGTCTG ATGACAAAAA GTGGAAGTTA CAGCTACATG AGCTGACTAA ACTTCCTGCC 
TTCGTGAGAG TTGTATCTGC AGGAAATCTT TTAAGCCACG TTGGTCATAC TATACTGGGC 
ATGAACACAG TTCAACTATA CATGAAAGTT CCAGGAAGCA GAACACCAGG TCATCAAGAA 
AATAACAACT TCTGTTCAGT TAATATAAAT ATTGGCCCAG GTGACTGTGA ATGGTTTGTT 
GTTCCTGAAG GCTACTGGGG TGTTTTGAAT GACTTCTGTG AAAAAAATAA TTTGAATTTC 
TTAATGGGTT CTTGGTGGCC CAACCTTGAA GATCTATATG AAGCAAATGT TCCAGTGTAT 
AGGTTTATTC AGCGACCTGG AGATCTGGTC TGGATAAATG CTGGCACTGT TCATTGGGTT 
CAAGCTATTG GCTGGTGCAA CAATATTGCT TGGAATGTTG GTCCACTTAC AGCCTGTCAG 
TATAAGTTAG CAGTGGAACG TTATGAATGG AACAAGTTGC AAAATGTAAA GTCAATAGTA 
CCCATGGTTC ATCTTTCCTG GAATATGGCA CGAAATATCA AGGTTTCAGA TCCAAAGCTT 
TTTGAAATGA TTAAGTATTG TCTTCTGAGA ACGCTGAAGC AATGTCAGAC ATTGAGGGAA 
GCTCTAATTG CTGCAGGAAA AGAGATCATA TGGCACGGGC GGACAAAAGA AGAACCAGCT 
CATTATTGTA GTATTTGTGA GGTGGAGGTT TTTGATCTGC TTTTTGTCAC TAATGAGAGT 
AATTCTCGAA AAACCTACAT AGTACATTGC CAAGATTGTG CACGAAAAAC AAGTGGGAAT 
CTGGAAAATT TTGTGGTGCT AGAACAGTAC AAAATGGAGG ATCTGATGCA AGTCTATGAC 
CAATTTACAT TAGCTCCTCC ATTACCATCC GCCTCATCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006527658.1
CDS	643..5004
Translation	MKSCGVSLATAAAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARMKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATILQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKNCSNTSGLAARIKYLQAQLCNLPQGSLQNKTKLLPSIEEAWSLPIPAELTSRQGAMNTAQQACKPHHPNTEPVLGLSQTPISQQSLPLHMIPPSQVDDLSSPAKRKRTSSPTKNTSDNWSGGNAPPPVEQQTHSWCLTPQKLQHLEQLRANRNNLNPAQKLMLEQLESQFVLMQQHQMRQTGVAQVRPTGILNGPTVDSSLPTNSVSGQQPQLPLTRMPSVSQPGVHTACPRQTLANGPFSAGHVPCSTSRTLGSTDTVLIGNNHVTGSGSNGNVPYLQRNAPTLPHNRTNLTSSTEEPWKNQLSNSTQGLHKGPSSHLAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNTLTVPETSRQTGETPNSTASVEGLPNHVHQVMADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGPGTCDKVNNIHPTVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPIKTDLLLVSHRPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGKRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQNVKSIVPMVHLSWNMARNIKVSDPKLFEMIKYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLAPPLPSASS

Target ORF information:

RefSeq Version	XM_006527595.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus lysine (K)-specific demethylase 6A (Kdm6a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527595.3

ORF Insert Sequence:

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCCG CCGCCGCCGC CGCCGCTTTC 
GGTGATGAGG AAAAGAAAAT GGCGGCGGGA AAAGCGAGCG GCGAGAGCGA GGAGGCGTCC 
CCCAGCCTGA CAGCGGAGGA GAGGGAGGCG CTCGGCGGAC TGGACAGCCG CCTTTTCGGG 
TTCGTGAGGT TTCATGAAGA TGGCGCCAGG ATGAAGGCCC TGCTGGGCAA GGCTGTTCGC 
TGCTACGAAT CTCTAATCTT AAAAGCTGAA GGGAAAGTGG AGTCTGATTT CTTTTGTCAA 
TTAGGTCACT TCAACCTCTT ATTGGAAGAT TATCCAAAAG CATTATCTGC ATACCAGAGG 
TACTACAGTT TACAGTCTGA TTACTGGAAG AATGCTGCCT TTTTATATGG TCTTGGTTTG 
GTCTACTTCC ATTACAATGC ATTTCAGTGG GCTATTAAAG CATTTCAGGA GGTGCTTTAT 
GTCGATCCCA GCTTTTGTCG AGCCAAGGAA ATTCATTTAC GACTTGGGCT TATGTTCAAA 
GTGAACACAG ACTATGAGTC TAGTTTAAAG CATTTTCAGT TAGCTTTGGT TGACTGTAAT 
CCCTGCACTT TGTCCAATGC TGAAATTCAG TTTCACATTG CCCACTTATA TGAAACCCAG 
AGGAAGTATC ATTCTGCAAA AGAAGCTTAT GAGCAACTTT TGCAGACAGA AAACCTTTCT 
GCACAAGTAA AAGCAACTAT TTTACAACAA TTAGGTTGGA TGCATCACAC TGTGGATCTC 
CTGGGAGATA AGGCCACCAA GGAAAGTTAT GCTATTCAGT ATCTCCAGAA GTCCTTGGAA 
GCAGATCCAA ATTCTGGCCA GTCCTGGTAT TTCCTTGGAA GGTGCTATTC AAGTATTGGG 
AAAGTTCAGG ATGCCTTTAT ATCTTACAGG CAATCTATTG ATAAATCAGA AGCAAGTGCA 
GATACATGGT GTTCAATAGG TGTGCTCTAT CAACAGCAAA ATCAGCCTAT GGATGCTTTG 
CAAGCTTATA TTTGTGCTGT ACAATTGGAC CACGGTCATG CTGCAGCCTG GATGGATCTA 
GGCACTCTCT ATGAATCCTG CAACCAACCT CAGGATGCTA TTAAATGCTA TTTAAATGCA 
ACTAGAAGCA AAAATTGTAG TAATACCTCT GGACTTGCAG CACGAATTAA GTATTTACAG 
GCTCAGTTGT GTAACCTTCC ACAAGGTAGT CTACAGAATA AAACTAAATT ACTTCCTAGT 
ATTGAGGAGG CATGGAGCCT ACCAATCCCC GCAGAGCTTA CCTCCAGGCA GGGTGCCATG 
AACACAGCAC AGCAGGCATG TAAACCTCAT CATCCAAATA CTGAACCTGT ATTAGGCCTC 
AGTCAAACAC CAATTTCACA GCAGTCCTTG CCACTACACA TGATTCCTCC TAGCCAAGTA 
GATGACCTGT CCAGTCCTGC CAAGAGGAAA AGAACATCTA GTCCAACAAA GAATACTTCT 
GATAATTGGA GTGGTGGCAA TGCACCACCT CCAGTAGAAC AACAAACTCA TTCATGGTGT 
TTGACACCAC AGAAATTACA GCACTTGGAA CAGCTCCGAG CAAACAGAAA TAATTTAAAT 
CCAGCACAGA AACTAATGCT GGAACAGCTG GAAAGTCAGT TTGTCTTAAT GCAGCAACAC 
CAAATGAGAC AAACAGGAGT TGCACAGGTA CGGCCTACTG GAATTCTTAA TGGGCCAACA 
GTTGACTCAT CACTGCCTAC AAACTCAGTT TCTGGCCAGC AGCCACAGCT TCCTCTGACC 
AGAATGCCTA GTGTCTCTCA GCCTGGAGTC CACACTGCCT GTCCTAGGCA GACTTTGGCC 
AATGGACCCT TTTCTGCAGG CCATGTTCCC TGTAGCACAT CAAGAACACT GGGAAGTACA 
GACACTGTTT TGATAGGCAA TAATCATGTA ACAGGAAGTG GAAGTAATGG AAACGTGCCT 
TACCTGCAGC GAAACGCACC CACTCTACCT CATAACCGCA CAAACCTGAC CAGCAGCACA 
GAGGAGCCGT GGAAAAACCA ACTATCTAAC TCCACTCAGG GGCTTCACAA AGGTCCGAGT 
TCACATTTGG CAGGTCCTAA TGGTGAACGA CCTCTATCTT CCACTGGGCC TTCCCAGCAT 
CTCCAGGCAG CTGGCTCTGG TATTCAGAAT CAGAATGGAC ATCCCACCCT GCCTAGCAAT 
TCAGTAACAC AGGGGGCTGC TCTCAATCAC CTCTCCTCTC ACACTGCTAC CTCAGGTGGA 
CAACAAGGCA TTACCTTAAC CAAAGAGAGC AAGCCTTCAG GAAACACATT GACGGTGCCT 
GAAACAAGCA GGCAAACTGG AGAGACACCT AACAGCACTG CCAGTGTTGA GGGACTTCCT 
AATCATGTCC ATCAGGTGAT GGCAGATGCT GTTTGCAGTC CTAGCCATGG AGATTCTAAG 
TCACCAGGTT TACTAAGTTC AGACAATCCT CAGCTCTCTG CCTTGTTGAT GGGAAAAGCT 
AATAACAATG TGGGTCCTGG AACCTGTGAC AAAGTCAATA ACATCCACCC AACTGTCCAT 
ACAAAGACTG ATAATTCTGT TGCCTCTTCA CCATCTTCAG CCATTTCCAC AGCAACACCT 
TCTCCTAAGT CCACTGAACA GACAACCACA AACAGTGTTA CCAGCCTTAA CAGCCCTCAC 
AGTGGGCTGC ACACAATTAA TGGAGAAGGA ATGGAAGAAT CTCAGAGCCC CATTAAAACA 
GATCTGCTTC TAGTTAGCCA CAGACCTAGT CCTCAGATCA TACCATCAAT GTCTGTGTCC 
ATATATCCCA GCTCAGCAGA AGTTCTGAAA GCTTGCAGGA ATCTAGGTAA AAACGGCCTG 
TCTAATAGTA GCATTCTGTT GGATAAATGT CCGCCTCCAA GACCACCATC CTCACCATAC 
CCTCCCTTGC CAAAGGACAA GTTGAATCCA CCTACACCTA GTATTTATTT GGAAAATAAA 
CGTGATGCTT TCTTTCCTCC ATTACATCAA TTTTGTACAA ACCCAAACAA CCCTGTTACA 
GTAATACGTG GCCTTGCTGG AGCTCTTAAA TTAGACTTGG GACTTTTCTC TACTAAAACT 
TTGGTGGAAG CTAACAATGA ACATATGGTA GAAGTGAGGA CACAGTTGTT ACAACCAGCA 
GATGAAAATT GGGACCCTAC TGGAACCAAG AAAATCTGGC ACTGTGAAAG TAATAGATCT 
CATACTACAA TTGCTAAATA TGCTCAGTAC CAGGCCTCCT CATTCCAAGA ATCATTGAGA 
GAAGAAAATG AGAAAAGAAG TCACCATAAA GACCACTCAG ACAGTGAATC TACATCATCA 
GATAATTCTG GGAAAAGAAG AAAAGGACCC TTTAAAACCA TTAAGTTTGG GACCAACATT 
GACCTGTCTG ATGACAAAAA GTGGAAGTTA CAGCTACATG AGCTGACTAA ACTTCCTGCC 
TTCGTGAGAG TTGTATCTGC AGGAAATCTT TTAAGCCACG TTGGTCATAC TATACTGGGC 
ATGAACACAG TTCAACTATA CATGAAAGTT CCAGGAAGCA GAACACCAGG TCATCAAGAA 
AATAACAACT TCTGTTCAGT TAATATAAAT ATTGGCCCAG GTGACTGTGA ATGGTTTGTT 
GTTCCTGAAG GCTACTGGGG TGTTTTGAAT GACTTCTGTG AAAAAAATAA TTTGAATTTC 
TTAATGGGTT CTTGGTGGCC CAACCTTGAA GATCTATATG AAGCAAATGT TCCAGTGTAT 
AGGTTTATTC AGCGACCTGG AGATCTGGTC TGGATAAATG CTGGCACTGT TCATTGGGTT 
CAAGCTATTG GCTGGTGCAA CAATATTGCT TGGAATGTTG GTCCACTTAC AGCCTGTCAG 
TATAAGTTAG CAGTGGAACG TTATGAATGG AACAAGTTGC AAAATGTAAA GTCAATAGTA 
CCCATGGTTC ATCTTTCCTG GAATATGGCA CGAAATATCA AGGTTTCAGA TCCAAAGCTT 
TTTGAAATGA TTAAGTATTG TCTTCTGAGA ACGCTGAAGC AATGTCAGAC ATTGAGGGAA 
GCTCTAATTG CTGCAGGAAA AGAGATCATA TGGCACGGGC GGACAAAAGA AGAACCAGCT 
CATTATTGTA GTATTTGTGA GGTGGAGGTT TTTGATCTGC TTTTTGTCAC TAATGAGAGT 
AATTCTCGAA AAACCTACAT AGTACATTGC CAAGATTGTG CACGAAAAAC AAGTGGGAAT 
CTGGAAAATT TTGTGGTGCT AGAACAGTAC AAAATGGAGG ATCTGATGCA AGTCTATGAC 
CAATTTACAT TAGCTCCTCC ATTACCATCC GCCTCATCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kdm6a ( XM_006527596.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527659.1 Mus musculus lysine (K)-specific demethylase 6A (Kdm6a), transcript variant X4, mRNA.

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CloneID	OMu55893
Clone ID Related Accession (Same CDS sequence)	XM_006527596.3 , XM_006527596.4
Accession Version	XM_006527596.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	lysine-specific demethylase 6A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006527596.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCCG CCGCCGCCGC CGCCGCTTTC 
GGTGATGAGG AAAAGAAAAT GGCGGCGGGA AAAGCGAGCG GCGAGAGCGA GGAGGCGTCC 
CCCAGCCTGA CAGCGGAGGA GAGGGAGGCG CTCGGCGGAC TGGACAGCCG CCTTTTCGGG 
TTCGTGAGGT TTCATGAAGA TGGCGCCAGG ATGAAGGCCC TGCTGGGCAA GGCTGTTCGC 
TGCTACGAAT CTCTAATCTT AAAAGCTGAA GGGAAAGTGG AGTCTGATTT CTTTTGTCAA 
TTAGGTCACT TCAACCTCTT ATTGGAAGAT TATCCAAAAG CATTATCTGC ATACCAGAGG 
TACTACAGTT TACAGTCTGA TTACTGGAAG AATGCTGCCT TTTTATATGG TCTTGGTTTG 
GTCTACTTCC ATTACAATGC ATTTCAGTGG GCTATTAAAG CATTTCAGGA GGTGCTTTAT 
GTCGATCCCA GCTTTTGTCG AGCCAAGGAA ATTCATTTAC GACTTGGGCT TATGTTCAAA 
GTGAACACAG ACTATGAGTC TAGTTTAAAG CATTTTCAGT TAGCTTTGGT TGACTGTAAT 
CCCTGCACTT TGTCCAATGC TGAAATTCAG TTTCACATTG CCCACTTATA TGAAACCCAG 
AGGAAGTATC ATTCTGCAAA AGAAGCTTAT GAGCAACTTT TGCAGACAGA AAACCTTTCT 
GCACAAGTAA AAGCAACTAT TTTACAACAA TTAGGTTGGA TGCATCACAC TGTGGATCTC 
CTGGGAGATA AGGCCACCAA GGAAAGTTAT GCTATTCAGT ATCTCCAGAA GTCCTTGGAA 
GCAGATCCAA ATTCTGGCCA GTCCTGGTAT TTCCTTGGAA GGTGCTATTC AAGTATTGGG 
AAAGTTCAGG ATGCCTTTAT ATCTTACAGG CAATCTATTG ATAAATCAGA AGCAAGTGCA 
GATACATGGT GTTCAATAGG TGTGCTCTAT CAACAGCAAA ATCAGCCTAT GGATGCTTTG 
CAAGCTTATA TTTGTGCTGT ACAATTGGAC CACGGTCATG CTGCAGCCTG GATGGATCTA 
GGCACTCTCT ATGAATCCTG CAACCAACCT CAGGATGCTA TTAAATGCTA TTTAAATGCA 
ACTAGAAGCA AAAATTGTAG TAATACCTCT GGACTTGCAG CACGAATTAA GTATTTACAG 
GCATGTAAAC CTCATCATCC AAATACTGAA CCTGTATTAG GCCTCAGTCA AACACCAATT 
TCACAGCAGT CCTTGCCACT ACACATGATT CCTCCTAGCC AAGTAGATGA CCTGTCCAGT 
CCTGCCAAGA GGAAAAGAAC ATCTAGTCCA ACAAAGAATA CTTCTGATAA TTGGAGTGGT 
GGCAATGCAC CACCTCCAGT AGAACAACAA ACTCATTCAT GGTGTTTGAC ACCACAGAAA 
TTACAGCACT TGGAACAGCT CCGAGCAAAC AGAAATAATT TAAATCCAGC ACAGAAACTA 
ATGCTGGAAC AGCTGGAAAG TCAGTTTGTC TTAATGCAGC AACACCAAAT GAGACAAACA 
GGAGTTGCAC AGGTACGGCC TACTGGAATT CTTAATGGGC CAACAGTTGA CTCATCACTG 
CCTACAAACT CAGTTTCTGG CCAGCAGCCA CAGCTTCCTC TGACCAGAAT GCCTAGTGTC 
TCTCAGCCTG GAGTCCACAC TGCCTGTCCT AGGCAGACTT TGGCCAATGG ACCCTTTTCT 
GCAGGCCATG TTCCCTGTAG CACATCAAGA ACACTGGGAA GTACAGACAC TGTTTTGATA 
GGCAATAATC ATGTAACAGG AAGTGGAAGT AATGGAAACG TGCCTTACCT GCAGCGAAAC 
GCACCCACTC TACCTCATAA CCGCACAAAC CTGACCAGCA GCACAGAGGA GCCGTGGAAA 
AACCAACTAT CTAACTCCAC TCAGGGGCTT CACAAAGGTC CGAGTTCACA TTTGGCAGGT 
CCTAATGGTG AACGACCTCT ATCTTCCACT GGGCCTTCCC AGCATCTCCA GGCAGCTGGC 
TCTGGTATTC AGAATCAGAA TGGACATCCC ACCCTGCCTA GCAATTCAGT AACACAGGGG 
GCTGCTCTCA ATCACCTCTC CTCTCACACT GCTACCTCAG GTGGACAACA AGGCATTACC 
TTAACCAAAG AGAGCAAGCC TTCAGGAAAC ACATTGACGG TGCCTGAAAC AAGCAGGCAA 
ACTGGAGAGA CACCTAACAG CACTGCCAGT GTTGAGGGAC TTCCTAATCA TGTCCATCAG 
GTGATGGCAG ATGCTGTTTG CAGTCCTAGC CATGGAGATT CTAAGTCACC AGGTTTACTA 
AGTTCAGACA ATCCTCAGCT CTCTGCCTTG TTGATGGGAA AAGCTAATAA CAATGTGGGT 
CCTGGAACCT GTGACAAAGT CAATAACATC CACCCAACTG TCCATACAAA GACTGATAAT 
TCTGTTGCCT CTTCACCATC TTCAGCCATT TCCACAGCAA CACCTTCTCC TAAGTCCACT 
GAACAGACAA CCACAAACAG TGTTACCAGC CTTAACAGCC CTCACAGTGG GCTGCACACA 
ATTAATGGAG AAGGAATGGA AGAATCTCAG AGCCCCATTA AAACAGATCT GCTTCTAGTT 
AGCCACAGAC CTAGTCCTCA GATCATACCA TCAATGTCTG TGTCCATATA TCCCAGCTCA 
GCAGAAGTTC TGAAAGCTTG CAGGAATCTA GGTAAAAACG GCCTGTCTAA TAGTAGCATT 
CTGTTGGATA AATGTCCGCC TCCAAGACCA CCATCCTCAC CATACCCTCC CTTGCCAAAG 
GACAAGTTGA ATCCACCTAC ACCTAGTATT TATTTGGAAA ATAAACGTGA TGCTTTCTTT 
CCTCCATTAC ATCAATTTTG TACAAACCCA AACAACCCTG TTACAGTAAT ACGTGGCCTT 
GCTGGAGCTC TTAAATTAGA CTTGGGACTT TTCTCTACTA AAACTTTGGT GGAAGCTAAC 
AATGAACATA TGGTAGAAGT GAGGACACAG TTGTTACAAC CAGCAGATGA AAATTGGGAC 
CCTACTGGAA CCAAGAAAAT CTGGCACTGT GAAAGTAATA GATCTCATAC TACAATTGCT 
AAATATGCTC AGTACCAGGC CTCCTCATTC CAAGAATCAT TGAGAGAAGA AAATGAGAAA 
AGAAGTCACC ATAAAGACCA CTCAGACAGT GAATCTACAT CATCAGATAA TTCTGGGAAA 
AGAAGAAAAG GACCCTTTAA AACCATTAAG TTTGGGACCA ACATTGACCT GTCTGATGAC 
AAAAAGTGGA AGTTACAGCT ACATGAGCTG ACTAAACTTC CTGCCTTCGT GAGAGTTGTA 
TCTGCAGGAA ATCTTTTAAG CCACGTTGGT CATACTATAC TGGGCATGAA CACAGTTCAA 
CTATACATGA AAGTTCCAGG AAGCAGAACA CCAGGTCATC AAGAAAATAA CAACTTCTGT 
TCAGTTAATA TAAATATTGG CCCAGGTGAC TGTGAATGGT TTGTTGTTCC TGAAGGCTAC 
TGGGGTGTTT TGAATGACTT CTGTGAAAAA AATAATTTGA ATTTCTTAAT GGGTTCTTGG 
TGGCCCAACC TTGAAGATCT ATATGAAGCA AATGTTCCAG TGTATAGGTT TATTCAGCGA 
CCTGGAGATC TGGTCTGGAT AAATGCTGGC ACTGTTCATT GGGTTCAAGC TATTGGCTGG 
TGCAACAATA TTGCTTGGAA TGTTGGTCCA CTTACAGCCT GTCAGTATAA GTTAGCAGTG 
GAACGTTATG AATGGAACAA GTTGCAAAAT GTAAAGTCAA TAGTACCCAT GGTTCATCTT 
TCCTGGAATA TGGCACGAAA TATCAAGGTT TCAGATCCAA AGCTTTTTGA AATGATTAAG 
TATTGTCTTC TGAGAACGCT GAAGCAATGT CAGACATTGA GGGAAGCTCT AATTGCTGCA 
GGAAAAGAGA TCATATGGCA CGGGCGGACA AAAGAAGAAC CAGCTCATTA TTGTAGTATT 
TGTGAGGTGG AGGTTTTTGA TCTGCTTTTT GTCACTAATG AGAGTAATTC TCGAAAAACC 
TACATAGTAC ATTGCCAAGA TTGTGCACGA AAAACAAGTG GGAATCTGGA AAATTTTGTG 
GTGCTAGAAC AGTACAAAAT GGAGGATCTG ATGCAAGTCT ATGACCAATT TACATTAGTA 
AGTGAAATCA ACATGCTCCT CCATTACCAT CCGCCTCATC TTGATATTGT TCCATGGACA 
TTAAACATGA GACCTTTTCT GCTATTCAGA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006527659.1
CDS	643..4938
Translation	MKSCGVSLATAAAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARMKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATILQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKNCSNTSGLAARIKYLQACKPHHPNTEPVLGLSQTPISQQSLPLHMIPPSQVDDLSSPAKRKRTSSPTKNTSDNWSGGNAPPPVEQQTHSWCLTPQKLQHLEQLRANRNNLNPAQKLMLEQLESQFVLMQQHQMRQTGVAQVRPTGILNGPTVDSSLPTNSVSGQQPQLPLTRMPSVSQPGVHTACPRQTLANGPFSAGHVPCSTSRTLGSTDTVLIGNNHVTGSGSNGNVPYLQRNAPTLPHNRTNLTSSTEEPWKNQLSNSTQGLHKGPSSHLAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNTLTVPETSRQTGETPNSTASVEGLPNHVHQVMADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGPGTCDKVNNIHPTVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPIKTDLLLVSHRPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGKRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQNVKSIVPMVHLSWNMARNIKVSDPKLFEMIKYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLVSEINMLLHYHPPHLDIVPWTLNMRPFLLFRK

Target ORF information:

RefSeq Version	XM_006527596.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus lysine (K)-specific demethylase 6A (Kdm6a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527596.3

ORF Insert Sequence:

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCCG CCGCCGCCGC CGCCGCTTTC 
GGTGATGAGG AAAAGAAAAT GGCGGCGGGA AAAGCGAGCG GCGAGAGCGA GGAGGCGTCC 
CCCAGCCTGA CAGCGGAGGA GAGGGAGGCG CTCGGCGGAC TGGACAGCCG CCTTTTCGGG 
TTCGTGAGGT TTCATGAAGA TGGCGCCAGG ATGAAGGCCC TGCTGGGCAA GGCTGTTCGC 
TGCTACGAAT CTCTAATCTT AAAAGCTGAA GGGAAAGTGG AGTCTGATTT CTTTTGTCAA 
TTAGGTCACT TCAACCTCTT ATTGGAAGAT TATCCAAAAG CATTATCTGC ATACCAGAGG 
TACTACAGTT TACAGTCTGA TTACTGGAAG AATGCTGCCT TTTTATATGG TCTTGGTTTG 
GTCTACTTCC ATTACAATGC ATTTCAGTGG GCTATTAAAG CATTTCAGGA GGTGCTTTAT 
GTCGATCCCA GCTTTTGTCG AGCCAAGGAA ATTCATTTAC GACTTGGGCT TATGTTCAAA 
GTGAACACAG ACTATGAGTC TAGTTTAAAG CATTTTCAGT TAGCTTTGGT TGACTGTAAT 
CCCTGCACTT TGTCCAATGC TGAAATTCAG TTTCACATTG CCCACTTATA TGAAACCCAG 
AGGAAGTATC ATTCTGCAAA AGAAGCTTAT GAGCAACTTT TGCAGACAGA AAACCTTTCT 
GCACAAGTAA AAGCAACTAT TTTACAACAA TTAGGTTGGA TGCATCACAC TGTGGATCTC 
CTGGGAGATA AGGCCACCAA GGAAAGTTAT GCTATTCAGT ATCTCCAGAA GTCCTTGGAA 
GCAGATCCAA ATTCTGGCCA GTCCTGGTAT TTCCTTGGAA GGTGCTATTC AAGTATTGGG 
AAAGTTCAGG ATGCCTTTAT ATCTTACAGG CAATCTATTG ATAAATCAGA AGCAAGTGCA 
GATACATGGT GTTCAATAGG TGTGCTCTAT CAACAGCAAA ATCAGCCTAT GGATGCTTTG 
CAAGCTTATA TTTGTGCTGT ACAATTGGAC CACGGTCATG CTGCAGCCTG GATGGATCTA 
GGCACTCTCT ATGAATCCTG CAACCAACCT CAGGATGCTA TTAAATGCTA TTTAAATGCA 
ACTAGAAGCA AAAATTGTAG TAATACCTCT GGACTTGCAG CACGAATTAA GTATTTACAG 
GCATGTAAAC CTCATCATCC AAATACTGAA CCTGTATTAG GCCTCAGTCA AACACCAATT 
TCACAGCAGT CCTTGCCACT ACACATGATT CCTCCTAGCC AAGTAGATGA CCTGTCCAGT 
CCTGCCAAGA GGAAAAGAAC ATCTAGTCCA ACAAAGAATA CTTCTGATAA TTGGAGTGGT 
GGCAATGCAC CACCTCCAGT AGAACAACAA ACTCATTCAT GGTGTTTGAC ACCACAGAAA 
TTACAGCACT TGGAACAGCT CCGAGCAAAC AGAAATAATT TAAATCCAGC ACAGAAACTA 
ATGCTGGAAC AGCTGGAAAG TCAGTTTGTC TTAATGCAGC AACACCAAAT GAGACAAACA 
GGAGTTGCAC AGGTACGGCC TACTGGAATT CTTAATGGGC CAACAGTTGA CTCATCACTG 
CCTACAAACT CAGTTTCTGG CCAGCAGCCA CAGCTTCCTC TGACCAGAAT GCCTAGTGTC 
TCTCAGCCTG GAGTCCACAC TGCCTGTCCT AGGCAGACTT TGGCCAATGG ACCCTTTTCT 
GCAGGCCATG TTCCCTGTAG CACATCAAGA ACACTGGGAA GTACAGACAC TGTTTTGATA 
GGCAATAATC ATGTAACAGG AAGTGGAAGT AATGGAAACG TGCCTTACCT GCAGCGAAAC 
GCACCCACTC TACCTCATAA CCGCACAAAC CTGACCAGCA GCACAGAGGA GCCGTGGAAA 
AACCAACTAT CTAACTCCAC TCAGGGGCTT CACAAAGGTC CGAGTTCACA TTTGGCAGGT 
CCTAATGGTG AACGACCTCT ATCTTCCACT GGGCCTTCCC AGCATCTCCA GGCAGCTGGC 
TCTGGTATTC AGAATCAGAA TGGACATCCC ACCCTGCCTA GCAATTCAGT AACACAGGGG 
GCTGCTCTCA ATCACCTCTC CTCTCACACT GCTACCTCAG GTGGACAACA AGGCATTACC 
TTAACCAAAG AGAGCAAGCC TTCAGGAAAC ACATTGACGG TGCCTGAAAC AAGCAGGCAA 
ACTGGAGAGA CACCTAACAG CACTGCCAGT GTTGAGGGAC TTCCTAATCA TGTCCATCAG 
GTGATGGCAG ATGCTGTTTG CAGTCCTAGC CATGGAGATT CTAAGTCACC AGGTTTACTA 
AGTTCAGACA ATCCTCAGCT CTCTGCCTTG TTGATGGGAA AAGCTAATAA CAATGTGGGT 
CCTGGAACCT GTGACAAAGT CAATAACATC CACCCAACTG TCCATACAAA GACTGATAAT 
TCTGTTGCCT CTTCACCATC TTCAGCCATT TCCACAGCAA CACCTTCTCC TAAGTCCACT 
GAACAGACAA CCACAAACAG TGTTACCAGC CTTAACAGCC CTCACAGTGG GCTGCACACA 
ATTAATGGAG AAGGAATGGA AGAATCTCAG AGCCCCATTA AAACAGATCT GCTTCTAGTT 
AGCCACAGAC CTAGTCCTCA GATCATACCA TCAATGTCTG TGTCCATATA TCCCAGCTCA 
GCAGAAGTTC TGAAAGCTTG CAGGAATCTA GGTAAAAACG GCCTGTCTAA TAGTAGCATT 
CTGTTGGATA AATGTCCGCC TCCAAGACCA CCATCCTCAC CATACCCTCC CTTGCCAAAG 
GACAAGTTGA ATCCACCTAC ACCTAGTATT TATTTGGAAA ATAAACGTGA TGCTTTCTTT 
CCTCCATTAC ATCAATTTTG TACAAACCCA AACAACCCTG TTACAGTAAT ACGTGGCCTT 
GCTGGAGCTC TTAAATTAGA CTTGGGACTT TTCTCTACTA AAACTTTGGT GGAAGCTAAC 
AATGAACATA TGGTAGAAGT GAGGACACAG TTGTTACAAC CAGCAGATGA AAATTGGGAC 
CCTACTGGAA CCAAGAAAAT CTGGCACTGT GAAAGTAATA GATCTCATAC TACAATTGCT 
AAATATGCTC AGTACCAGGC CTCCTCATTC CAAGAATCAT TGAGAGAAGA AAATGAGAAA 
AGAAGTCACC ATAAAGACCA CTCAGACAGT GAATCTACAT CATCAGATAA TTCTGGGAAA 
AGAAGAAAAG GACCCTTTAA AACCATTAAG TTTGGGACCA ACATTGACCT GTCTGATGAC 
AAAAAGTGGA AGTTACAGCT ACATGAGCTG ACTAAACTTC CTGCCTTCGT GAGAGTTGTA 
TCTGCAGGAA ATCTTTTAAG CCACGTTGGT CATACTATAC TGGGCATGAA CACAGTTCAA 
CTATACATGA AAGTTCCAGG AAGCAGAACA CCAGGTCATC AAGAAAATAA CAACTTCTGT 
TCAGTTAATA TAAATATTGG CCCAGGTGAC TGTGAATGGT TTGTTGTTCC TGAAGGCTAC 
TGGGGTGTTT TGAATGACTT CTGTGAAAAA AATAATTTGA ATTTCTTAAT GGGTTCTTGG 
TGGCCCAACC TTGAAGATCT ATATGAAGCA AATGTTCCAG TGTATAGGTT TATTCAGCGA 
CCTGGAGATC TGGTCTGGAT AAATGCTGGC ACTGTTCATT GGGTTCAAGC TATTGGCTGG 
TGCAACAATA TTGCTTGGAA TGTTGGTCCA CTTACAGCCT GTCAGTATAA GTTAGCAGTG 
GAACGTTATG AATGGAACAA GTTGCAAAAT GTAAAGTCAA TAGTACCCAT GGTTCATCTT 
TCCTGGAATA TGGCACGAAA TATCAAGGTT TCAGATCCAA AGCTTTTTGA AATGATTAAG 
TATTGTCTTC TGAGAACGCT GAAGCAATGT CAGACATTGA GGGAAGCTCT AATTGCTGCA 
GGAAAAGAGA TCATATGGCA CGGGCGGACA AAAGAAGAAC CAGCTCATTA TTGTAGTATT 
TGTGAGGTGG AGGTTTTTGA TCTGCTTTTT GTCACTAATG AGAGTAATTC TCGAAAAACC 
TACATAGTAC ATTGCCAAGA TTGTGCACGA AAAACAAGTG GGAATCTGGA AAATTTTGTG 
GTGCTAGAAC AGTACAAAAT GGAGGATCTG ATGCAAGTCT ATGACCAATT TACATTAGTA 
AGTGAAATCA ACATGCTCCT CCATTACCAT CCGCCTCATC TTGATATTGT TCCATGGACA 
TTAAACATGA GACCTTTTCT GCTATTCAGA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kdm6a ( XM_006527597.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527660.1 Mus musculus lysine (K)-specific demethylase 6A (Kdm6a), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu55894
Clone ID Related Accession (Same CDS sequence)	XM_006527597.4 , XM_006527597.3
Accession Version	XM_006527597.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	lysine-specific demethylase 6A isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006527597.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCCG CCGCCGCCGC CGCCGCTTTC 
GGTGATGAGG AAAAGAAAAT GGCGGCGGGA AAAGCGAGCG GCGAGAGCGA GGAGGCGTCC 
CCCAGCCTGA CAGCGGAGGA GAGGGAGGCG CTCGGCGGAC TGGACAGCCG CCTTTTCGGG 
TTCGTGAGGT TTCATGAAGA TGGCGCCAGG ATGAAGGCCC TGCTGGGCAA GGCTGTTCGC 
TGCTACGAAT CTCTAATCTT AAAAGCTGAA GGGAAAGTGG AGTCTGATTT CTTTTGTCAA 
TTAGGTCACT TCAACCTCTT ATTGGAAGAT TATCCAAAAG CATTATCTGC ATACCAGAGG 
TACTACAGTT TACAGTCTGA TTACTGGAAG AATGCTGCCT TTTTATATGG TCTTGGTTTG 
GTCTACTTCC ATTACAATGC ATTTCAGTGG GCTATTAAAG CATTTCAGGA GGTGCTTTAT 
GTCGATCCCA GCTTTTGTCG AGCCAAGGAA ATTCATTTAC GACTTGGGCT TATGTTCAAA 
GTGAACACAG ACTATGAGTC TAGTTTAAAG CATTTTCAGT TAGCTTTGGT TGACTGTAAT 
CCCTGCACTT TGTCCAATGC TGAAATTCAG TTTCACATTG CCCACTTATA TGAAACCCAG 
AGGAAGTATC ATTCTGCAAA AGAAGCTTAT GAGCAACTTT TGCAGACAGA AAACCTTTCT 
GCACAAGTAA AAGCAACTAT TTTACAACAA TTAGGTTGGA TGCATCACAC TGTGGATCTC 
CTGGGAGATA AGGCCACCAA GGAAAGTTAT GCTATTCAGT ATCTCCAGAA GTCCTTGGAA 
GCAGATCCAA ATTCTGGCCA GTCCTGGTAT TTCCTTGGAA GGTGCTATTC AAGTATTGGG 
AAAGTTCAGG ATGCCTTTAT ATCTTACAGG CAATCTATTG ATAAATCAGA AGCAAGTGCA 
GATACATGGT GTTCAATAGG TGTGCTCTAT CAACAGCAAA ATCAGCCTAT GGATGCTTTG 
CAAGCTTATA TTTGTGCTGT ACAATTGGAC CACGGTCATG CTGCAGCCTG GATGGATCTA 
GGCACTCTCT ATGAATCCTG CAACCAACCT CAGGATGCTA TTAAATGCTA TTTAAATGCA 
ACTAGAAGCA AAAATTGTAG TAATACCTCT GGACTTGCAG CACGAATTAA GTATTTACAG 
GCATGTAAAC CTCATCATCC AAATACTGAA CCTGTATTAG GCCTCAGTCA AACACCAATT 
TCACAGCAGT CCTTGCCACT ACACATGATT CCTCCTAGCC AAGTAGATGA CCTGTCCAGT 
CCTGCCAAGA GGAAAAGAAC ATCTAGTCCA ACAAAGAATA CTTCTGATAA TTGGAGTGGT 
GGCAATGCAC CACCTCCAGT AGAACAACAA ACTCATTCAT GGTGTTTGAC ACCACAGAAA 
TTACAGCACT TGGAACAGCT CCGAGCAAAC AGAAATAATT TAAATCCAGC ACAGAAACTA 
ATGCTGGAAC AGCTGGAAAG TCAGTTTGTC TTAATGCAGC AACACCAAAT GAGACAAACA 
GGAGTTGCAC AGGTACGGCC TACTGGAATT CTTAATGGGC CAACAGTTGA CTCATCACTG 
CCTACAAACT CAGTTTCTGG CCAGCAGCCA CAGCTTCCTC TGACCAGAAT GCCTAGTGTC 
TCTCAGCCTG GAGTCCACAC TGCCTGTCCT AGGCAGACTT TGGCCAATGG ACCCTTTTCT 
GCAGGCCATG TTCCCTGTAG CACATCAAGA ACACTGGGAA GTACAGACAC TGTTTTGATA 
GGCAATAATC ATGTAACAGG AAGTGGAAGT AATGGAAACG TGCCTTACCT GCAGCGAAAC 
GCACCCACTC TACCTCATAA CCGCACAAAC CTGACCAGCA GCACAGAGGA GCCGTGGAAA 
AACCAACTAT CTAACTCCAC TCAGGGGCTT CACAAAGGTC CGAGTTCACA TTTGGCAGGT 
CCTAATGGTG AACGACCTCT ATCTTCCACT GGGCCTTCCC AGCATCTCCA GGCAGCTGGC 
TCTGGTATTC AGAATCAGAA TGGACATCCC ACCCTGCCTA GCAATTCAGT AACACAGGGG 
GCTGCTCTCA ATCACCTCTC CTCTCACACT GCTACCTCAG GTGGACAACA AGGCATTACC 
TTAACCAAAG AGAGCAAGCC TTCAGGAAAC ACATTGACGG TGCCTGAAAC AAGCAGGCAA 
ACTGGAGAGA CACCTAACAG CACTGCCAGT GTTGAGGGAC TTCCTAATCA TGTCCATCAG 
GTGATGGCAG ATGCTGTTTG CAGTCCTAGC CATGGAGATT CTAAGTCACC AGGTTTACTA 
AGTTCAGACA ATCCTCAGCT CTCTGCCTTG TTGATGGGAA AAGCTAATAA CAATGTGGGT 
CCTGGAACCT GTGACAAAGT CAATAACATC CACCCAACTG TCCATACAAA GACTGATAAT 
TCTGTTGCCT CTTCACCATC TTCAGCCATT TCCACAGCAA CACCTTCTCC TAAGTCCACT 
GAACAGACAA CCACAAACAG TGTTACCAGC CTTAACAGCC CTCACAGTGG GCTGCACACA 
ATTAATGGAG AAGGAATGGA AGAATCTCAG AGCCCCATTA AAACAGATCT GCTTCTAGTT 
AGCCACAGAC CTAGTCCTCA GATCATACCA TCAATGTCTG TGTCCATATA TCCCAGCTCA 
GCAGAAGTTC TGAAAGCTTG CAGGAATCTA GGTAAAAACG GCCTGTCTAA TAGTAGCATT 
CTGTTGGATA AATGTCCGCC TCCAAGACCA CCATCCTCAC CATACCCTCC CTTGCCAAAG 
GACAAGTTGA ATCCACCTAC ACCTAGTATT TATTTGGAAA ATAAACGTGA TGCTTTCTTT 
CCTCCATTAC ATCAATTTTG TACAAACCCA AACAACCCTG TTACAGTAAT ACGTGGCCTT 
GCTGGAGCTC TTAAATTAGA CTTGGGACTT TTCTCTACTA AAACTTTGGT GGAAGCTAAC 
AATGAACATA TGGTAGAAGT GAGGACACAG TTGTTACAAC CAGCAGATGA AAATTGGGAC 
CCTACTGGAA CCAAGAAAAT CTGGCACTGT GAAAGTAATA GATCTCATAC TACAATTGCT 
AAATATGCTC AGTACCAGGC CTCCTCATTC CAAGAATCAT TGAGAGAAGA AAATGAGAAA 
AGAAGTCACC ATAAAGACCA CTCAGACAGT GAATCTACAT CATCAGATAA TTCTGGGAAA 
AGAAGAAAAG GACCCTTTAA AACCATTAAG TTTGGGACCA ACATTGACCT GTCTGATGAC 
AAAAAGTGGA AGTTACAGCT ACATGAGCTG ACTAAACTTC CTGCCTTCGT GAGAGTTGTA 
TCTGCAGGAA ATCTTTTAAG CCACGTTGGT CATACTATAC TGGGCATGAA CACAGTTCAA 
CTATACATGA AAGTTCCAGG AAGCAGAACA CCAGGTCATC AAGAAAATAA CAACTTCTGT 
TCAGTTAATA TAAATATTGG CCCAGGTGAC TGTGAATGGT TTGTTGTTCC TGAAGGCTAC 
TGGGGTGTTT TGAATGACTT CTGTGAAAAA AATAATTTGA ATTTCTTAAT GGGTTCTTGG 
TGGCCCAACC TTGAAGATCT ATATGAAGCA AATGTTCCAG TGTATAGGTT TATTCAGCGA 
CCTGGAGATC TGGTCTGGAT AAATGCTGGC ACTGTTCATT GGGTTCAAGC TATTGGCTGG 
TGCAACAATA TTGCTTGGAA TGTTGGTCCA CTTACAGCCT GTCAGTATAA GTTAGCAGTG 
GAACGTTATG AATGGAACAA GTTGCAAAAT GTAAAGTCAA TAGTACCCAT GGTTCATCTT 
TCCTGGAATA TGGCACGAAA TATCAAGGTT TCAGATCCAA AGCTTTTTGA AATGATTAAG 
TATTGTCTTC TGAGAACGCT GAAGCAATGT CAGACATTGA GGGAAGCTCT AATTGCTGCA 
GGAAAAGAGA TCATATGGCA CGGGCGGACA AAAGAAGAAC CAGCTCATTA TTGTAGTATT 
TGTGAGGTGG AGGTTTTTGA TCTGCTTTTT GTCACTAATG AGAGTAATTC TCGAAAAACC 
TACATAGTAC ATTGCCAAGA TTGTGCACGA AAAACAAGTG GGAATCTGGA AAATTTTGTG 
GTGCTAGAAC AGTACAAAAT GGAGGATCTG ATGCAAGTCT ATGACCAATT TACATTAGCT 
CCTCCATTAC CATCCGCCTC ATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006527660.1
CDS	643..4869
Translation	MKSCGVSLATAAAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARMKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATILQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKNCSNTSGLAARIKYLQACKPHHPNTEPVLGLSQTPISQQSLPLHMIPPSQVDDLSSPAKRKRTSSPTKNTSDNWSGGNAPPPVEQQTHSWCLTPQKLQHLEQLRANRNNLNPAQKLMLEQLESQFVLMQQHQMRQTGVAQVRPTGILNGPTVDSSLPTNSVSGQQPQLPLTRMPSVSQPGVHTACPRQTLANGPFSAGHVPCSTSRTLGSTDTVLIGNNHVTGSGSNGNVPYLQRNAPTLPHNRTNLTSSTEEPWKNQLSNSTQGLHKGPSSHLAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNTLTVPETSRQTGETPNSTASVEGLPNHVHQVMADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGPGTCDKVNNIHPTVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPIKTDLLLVSHRPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGKRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQNVKSIVPMVHLSWNMARNIKVSDPKLFEMIKYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLAPPLPSASS

Target ORF information:

RefSeq Version	XM_006527597.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus lysine (K)-specific demethylase 6A (Kdm6a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527597.3

ORF Insert Sequence:

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCCG CCGCCGCCGC CGCCGCTTTC 
GGTGATGAGG AAAAGAAAAT GGCGGCGGGA AAAGCGAGCG GCGAGAGCGA GGAGGCGTCC 
CCCAGCCTGA CAGCGGAGGA GAGGGAGGCG CTCGGCGGAC TGGACAGCCG CCTTTTCGGG 
TTCGTGAGGT TTCATGAAGA TGGCGCCAGG ATGAAGGCCC TGCTGGGCAA GGCTGTTCGC 
TGCTACGAAT CTCTAATCTT AAAAGCTGAA GGGAAAGTGG AGTCTGATTT CTTTTGTCAA 
TTAGGTCACT TCAACCTCTT ATTGGAAGAT TATCCAAAAG CATTATCTGC ATACCAGAGG 
TACTACAGTT TACAGTCTGA TTACTGGAAG AATGCTGCCT TTTTATATGG TCTTGGTTTG 
GTCTACTTCC ATTACAATGC ATTTCAGTGG GCTATTAAAG CATTTCAGGA GGTGCTTTAT 
GTCGATCCCA GCTTTTGTCG AGCCAAGGAA ATTCATTTAC GACTTGGGCT TATGTTCAAA 
GTGAACACAG ACTATGAGTC TAGTTTAAAG CATTTTCAGT TAGCTTTGGT TGACTGTAAT 
CCCTGCACTT TGTCCAATGC TGAAATTCAG TTTCACATTG CCCACTTATA TGAAACCCAG 
AGGAAGTATC ATTCTGCAAA AGAAGCTTAT GAGCAACTTT TGCAGACAGA AAACCTTTCT 
GCACAAGTAA AAGCAACTAT TTTACAACAA TTAGGTTGGA TGCATCACAC TGTGGATCTC 
CTGGGAGATA AGGCCACCAA GGAAAGTTAT GCTATTCAGT ATCTCCAGAA GTCCTTGGAA 
GCAGATCCAA ATTCTGGCCA GTCCTGGTAT TTCCTTGGAA GGTGCTATTC AAGTATTGGG 
AAAGTTCAGG ATGCCTTTAT ATCTTACAGG CAATCTATTG ATAAATCAGA AGCAAGTGCA 
GATACATGGT GTTCAATAGG TGTGCTCTAT CAACAGCAAA ATCAGCCTAT GGATGCTTTG 
CAAGCTTATA TTTGTGCTGT ACAATTGGAC CACGGTCATG CTGCAGCCTG GATGGATCTA 
GGCACTCTCT ATGAATCCTG CAACCAACCT CAGGATGCTA TTAAATGCTA TTTAAATGCA 
ACTAGAAGCA AAAATTGTAG TAATACCTCT GGACTTGCAG CACGAATTAA GTATTTACAG 
GCATGTAAAC CTCATCATCC AAATACTGAA CCTGTATTAG GCCTCAGTCA AACACCAATT 
TCACAGCAGT CCTTGCCACT ACACATGATT CCTCCTAGCC AAGTAGATGA CCTGTCCAGT 
CCTGCCAAGA GGAAAAGAAC ATCTAGTCCA ACAAAGAATA CTTCTGATAA TTGGAGTGGT 
GGCAATGCAC CACCTCCAGT AGAACAACAA ACTCATTCAT GGTGTTTGAC ACCACAGAAA 
TTACAGCACT TGGAACAGCT CCGAGCAAAC AGAAATAATT TAAATCCAGC ACAGAAACTA 
ATGCTGGAAC AGCTGGAAAG TCAGTTTGTC TTAATGCAGC AACACCAAAT GAGACAAACA 
GGAGTTGCAC AGGTACGGCC TACTGGAATT CTTAATGGGC CAACAGTTGA CTCATCACTG 
CCTACAAACT CAGTTTCTGG CCAGCAGCCA CAGCTTCCTC TGACCAGAAT GCCTAGTGTC 
TCTCAGCCTG GAGTCCACAC TGCCTGTCCT AGGCAGACTT TGGCCAATGG ACCCTTTTCT 
GCAGGCCATG TTCCCTGTAG CACATCAAGA ACACTGGGAA GTACAGACAC TGTTTTGATA 
GGCAATAATC ATGTAACAGG AAGTGGAAGT AATGGAAACG TGCCTTACCT GCAGCGAAAC 
GCACCCACTC TACCTCATAA CCGCACAAAC CTGACCAGCA GCACAGAGGA GCCGTGGAAA 
AACCAACTAT CTAACTCCAC TCAGGGGCTT CACAAAGGTC CGAGTTCACA TTTGGCAGGT 
CCTAATGGTG AACGACCTCT ATCTTCCACT GGGCCTTCCC AGCATCTCCA GGCAGCTGGC 
TCTGGTATTC AGAATCAGAA TGGACATCCC ACCCTGCCTA GCAATTCAGT AACACAGGGG 
GCTGCTCTCA ATCACCTCTC CTCTCACACT GCTACCTCAG GTGGACAACA AGGCATTACC 
TTAACCAAAG AGAGCAAGCC TTCAGGAAAC ACATTGACGG TGCCTGAAAC AAGCAGGCAA 
ACTGGAGAGA CACCTAACAG CACTGCCAGT GTTGAGGGAC TTCCTAATCA TGTCCATCAG 
GTGATGGCAG ATGCTGTTTG CAGTCCTAGC CATGGAGATT CTAAGTCACC AGGTTTACTA 
AGTTCAGACA ATCCTCAGCT CTCTGCCTTG TTGATGGGAA AAGCTAATAA CAATGTGGGT 
CCTGGAACCT GTGACAAAGT CAATAACATC CACCCAACTG TCCATACAAA GACTGATAAT 
TCTGTTGCCT CTTCACCATC TTCAGCCATT TCCACAGCAA CACCTTCTCC TAAGTCCACT 
GAACAGACAA CCACAAACAG TGTTACCAGC CTTAACAGCC CTCACAGTGG GCTGCACACA 
ATTAATGGAG AAGGAATGGA AGAATCTCAG AGCCCCATTA AAACAGATCT GCTTCTAGTT 
AGCCACAGAC CTAGTCCTCA GATCATACCA TCAATGTCTG TGTCCATATA TCCCAGCTCA 
GCAGAAGTTC TGAAAGCTTG CAGGAATCTA GGTAAAAACG GCCTGTCTAA TAGTAGCATT 
CTGTTGGATA AATGTCCGCC TCCAAGACCA CCATCCTCAC CATACCCTCC CTTGCCAAAG 
GACAAGTTGA ATCCACCTAC ACCTAGTATT TATTTGGAAA ATAAACGTGA TGCTTTCTTT 
CCTCCATTAC ATCAATTTTG TACAAACCCA AACAACCCTG TTACAGTAAT ACGTGGCCTT 
GCTGGAGCTC TTAAATTAGA CTTGGGACTT TTCTCTACTA AAACTTTGGT GGAAGCTAAC 
AATGAACATA TGGTAGAAGT GAGGACACAG TTGTTACAAC CAGCAGATGA AAATTGGGAC 
CCTACTGGAA CCAAGAAAAT CTGGCACTGT GAAAGTAATA GATCTCATAC TACAATTGCT 
AAATATGCTC AGTACCAGGC CTCCTCATTC CAAGAATCAT TGAGAGAAGA AAATGAGAAA 
AGAAGTCACC ATAAAGACCA CTCAGACAGT GAATCTACAT CATCAGATAA TTCTGGGAAA 
AGAAGAAAAG GACCCTTTAA AACCATTAAG TTTGGGACCA ACATTGACCT GTCTGATGAC 
AAAAAGTGGA AGTTACAGCT ACATGAGCTG ACTAAACTTC CTGCCTTCGT GAGAGTTGTA 
TCTGCAGGAA ATCTTTTAAG CCACGTTGGT CATACTATAC TGGGCATGAA CACAGTTCAA 
CTATACATGA AAGTTCCAGG AAGCAGAACA CCAGGTCATC AAGAAAATAA CAACTTCTGT 
TCAGTTAATA TAAATATTGG CCCAGGTGAC TGTGAATGGT TTGTTGTTCC TGAAGGCTAC 
TGGGGTGTTT TGAATGACTT CTGTGAAAAA AATAATTTGA ATTTCTTAAT GGGTTCTTGG 
TGGCCCAACC TTGAAGATCT ATATGAAGCA AATGTTCCAG TGTATAGGTT TATTCAGCGA 
CCTGGAGATC TGGTCTGGAT AAATGCTGGC ACTGTTCATT GGGTTCAAGC TATTGGCTGG 
TGCAACAATA TTGCTTGGAA TGTTGGTCCA CTTACAGCCT GTCAGTATAA GTTAGCAGTG 
GAACGTTATG AATGGAACAA GTTGCAAAAT GTAAAGTCAA TAGTACCCAT GGTTCATCTT 
TCCTGGAATA TGGCACGAAA TATCAAGGTT TCAGATCCAA AGCTTTTTGA AATGATTAAG 
TATTGTCTTC TGAGAACGCT GAAGCAATGT CAGACATTGA GGGAAGCTCT AATTGCTGCA 
GGAAAAGAGA TCATATGGCA CGGGCGGACA AAAGAAGAAC CAGCTCATTA TTGTAGTATT 
TGTGAGGTGG AGGTTTTTGA TCTGCTTTTT GTCACTAATG AGAGTAATTC TCGAAAAACC 
TACATAGTAC ATTGCCAAGA TTGTGCACGA AAAACAAGTG GGAATCTGGA AAATTTTGTG 
GTGCTAGAAC AGTACAAAAT GGAGGATCTG ATGCAAGTCT ATGACCAATT TACATTAGCT 
CCTCCATTAC CATCCGCCTC ATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kdm6a ( XM_006527599.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527662.1 Mus musculus lysine (K)-specific demethylase 6A (Kdm6a), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMu55896
Clone ID Related Accession (Same CDS sequence)	XM_006527599.3 , XM_006527599.4
Accession Version	XM_006527599.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4140bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	lysine-specific demethylase 6A isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006527599.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCCG CCGCCGCCGC CGCCGCTTTC 
GGTGATGAGG AAAAGAAAAT GGCGGCGGGA AAAGCGAGCG GCGAGAGCGA GGAGGCGTCC 
CCCAGCCTGA CAGCGGAGGA GAGGGAGGCG CTCGGCGGAC TGGACAGCCG CCTTTTCGGG 
TTCGTGAGGT TTCATGAAGA TGGCGCCAGG ATGAAGGCCC TGCTGGGCAA GGCTGTTCGC 
TGCTACGAAT CTCTAATCTT AAAAGCTGAA GGGAAAGTGG AGTCTGATTT CTTTTGTCAA 
TTAGGTCACT TCAACCTCTT ATTGGAAGAT TATCCAAAAG CATTATCTGC ATACCAGAGG 
TACTACAGTT TACAGTCTGA TTACTGGAAG AATGCTGCCT TTTTATATGG TCTTGGTTTG 
GTCTACTTCC ATTACAATGC ATTTCAGTGG GCTATTAAAG CATTTCAGGA GGTGCTTTAT 
GTCGATCCCA GCTTTTGTCG AGCCAAGGAA ATTCATTTAC GACTTGGGCT TATGTTCAAA 
GTGAACACAG ACTATGAGTC TAGTTTAAAG CATTTTCAGT TAGCTTTGGT TGACTGTAAT 
CCCTGCACTT TGTCCAATGC TGAAATTCAG TTTCACATTG CCCACTTATA TGAAACCCAG 
AGGAAGTATC ATTCTGCAAA AGAAGCTTAT GAGCAACTTT TGCAGACAGA AAACCTTTCT 
GCACAAGTAA AAGCAACTAT TTTACAACAA TTAGGTTGGA TGCATCACAC TGTGGATCTC 
CTGGGAGATA AGGCCACCAA GGAAAGTTAT GCTATTCAGT ATCTCCAGAA GTCCTTGGAA 
GCAGATCCAA ATTCTGGCCA GTCCTGGTAT TTCCTTGGAA GGTGCTATTC AAGTATTGGG 
AAAGTTCAGG ATGCCTTTAT ATCTTACAGG CAATCTATTG ATAAATCAGA AGCAAGTGCA 
GATACATGGT GTTCAATAGG TGTGCTCTAT CAACAGCAAA ATCAGCCTAT GGATGCTTTG 
CAAGCTTATA TTTGTGCTGT ACAATTGGAC CACGGTCATG CTGCAGCCTG GATGGATCTA 
GGCACTCTCT ATGAATCCTG CAACCAACCT CAGGATGCTA TTAAATGCTA TTTAAATGCA 
ACTAGAAGCA AAAATTGTAG TAATACCTCT GGACTTGCAG CACGAATTAA GTATTTACAG 
AATACTTCTG ATAATTGGAG TGGTGGCAAT GCACCACCTC CAGTAGAACA ACAAACTCAT 
TCATGGTGTT TGACACCACA GAAATTACAG CACTTGGAAC AGCTCCGAGC AAACAGAAAT 
AATTTAAATC CAGCACAGAA ACTAATGCTG GAACAGCTGG AAAGTCAGTT TGTCTTAATG 
CAGCAACACC AAATGAGACA AACAGGAGTT GCACAGGTAC GGCCTACTGG AATTCTTAAT 
GGGCCAACAG TTGACTCATC ACTGCCTACA AACTCAGTTT CTGGCCAGCA GCCACAGCTT 
CCTCTGACCA GAATGCCTAG TGTCTCTCAG CCTGGAGTCC ACACTGCCTG TCCTAGGCAG 
ACTTTGGCCA ATGGACCCTT TTCTGCAGGC CATGTTCCCT GTAGCACATC AAGAACACTG 
GGAAGTACAG ACACTGTTTT GATAGGCAAT AATCATGTAA CAGGAAGTGG AAGTAATGGA 
AACGTGCCTT ACCTGCAGCG AAACGCACCC ACTCTACCTC ATAACCGCAC AAACCTGACC 
AGCAGCACAG AGGAGCCGTG GAAAAACCAA CTATCTAACT CCACTCAGGG GCTTCACAAA 
GGTCCGAGTT CACATTTGGC AGGTCCTAAT GGTGAACGAC CTCTATCTTC CACTGGGCCT 
TCCCAGCATC TCCAGGCAGC TGGCTCTGGT ATTCAGAATC AGAATGGACA TCCCACCCTG 
CCTAGCAATT CAGTAACACA GGGGGCTGCT CTCAATCACC TCTCCTCTCA CACTGCTACC 
TCAGGTGGAC AACAAGGCAT TACCTTAACC AAAGAGAGCA AGCCTTCAGG AAACACATTG 
ACGGTGCCTG AAACAAGCAG GCAAACTGGA GAGACACCTA ACAGCACTGC CAGTGTTGAG 
GGACTTCCTA ATCATGTCCA TCAGGTGATG GCAGATGCTG TTTGCAGTCC TAGCCATGGA 
GATTCTAAGT CACCAGGTTT ACTAAGTTCA GACAATCCTC AGCTCTCTGC CTTGTTGATG 
GGAAAAGCTA ATAACAATGT GGGTCCTGGA ACCTGTGACA AAGTCAATAA CATCCACCCA 
ACTGTCCATA CAAAGACTGA TAATTCTGTT GCCTCTTCAC CATCTTCAGC CATTTCCACA 
GCAACACCTT CTCCTAAGTC CACTGAACAG ACAACCACAA ACAGTGTTAC CAGCCTTAAC 
AGCCCTCACA GTGGGCTGCA CACAATTAAT GGAGAAGGAA TGGAAGAATC TCAGAGCCCC 
ATTAAAACAG ATCTGCTTCT AGTTAGCCAC AGACCTAGTC CTCAGATCAT ACCATCAATG 
TCTGTGTCCA TATATCCCAG CTCAGCAGAA GTTCTGAAAG CTTGCAGGAA TCTAGGTAAA 
AACGGCCTGT CTAATAGTAG CATTCTGTTG GATAAATGTC CGCCTCCAAG ACCACCATCC 
TCACCATACC CTCCCTTGCC AAAGGACAAG TTGAATCCAC CTACACCTAG TATTTATTTG 
GAAAATAAAC GTGATGCTTT CTTTCCTCCA TTACATCAAT TTTGTACAAA CCCAAACAAC 
CCTGTTACAG TAATACGTGG CCTTGCTGGA GCTCTTAAAT TAGACTTGGG ACTTTTCTCT 
ACTAAAACTT TGGTGGAAGC TAACAATGAA CATATGGTAG AAGTGAGGAC ACAGTTGTTA 
CAACCAGCAG ATGAAAATTG GGACCCTACT GGAACCAAGA AAATCTGGCA CTGTGAAAGT 
AATAGATCTC ATACTACAAT TGCTAAATAT GCTCAGTACC AGGCCTCCTC ATTCCAAGAA 
TCATTGAGAG AAGAAAATGA GAAAAGAAGT CACCATAAAG ACCACTCAGA CAGTGAATCT 
ACATCATCAG ATAATTCTGG GAAAAGAAGA AAAGGACCCT TTAAAACCAT TAAGTTTGGG 
ACCAACATTG ACCTGTCTGA TGACAAAAAG TGGAAGTTAC AGCTACATGA GCTGACTAAA 
CTTCCTGCCT TCGTGAGAGT TGTATCTGCA GGAAATCTTT TAAGCCACGT TGGTCATACT 
ATACTGGGCA TGAACACAGT TCAACTATAC ATGAAAGTTC CAGGAAGCAG AACACCAGGT 
CATCAAGAAA ATAACAACTT CTGTTCAGTT AATATAAATA TTGGCCCAGG TGACTGTGAA 
TGGTTTGTTG TTCCTGAAGG CTACTGGGGT GTTTTGAATG ACTTCTGTGA AAAAAATAAT 
TTGAATTTCT TAATGGGTTC TTGGTGGCCC AACCTTGAAG ATCTATATGA AGCAAATGTT 
CCAGTGTATA GGTTTATTCA GCGACCTGGA GATCTGGTCT GGATAAATGC TGGCACTGTT 
CATTGGGTTC AAGCTATTGG CTGGTGCAAC AATATTGCTT GGAATGTTGG TCCACTTACA 
GCCTGTCAGT ATAAGTTAGC AGTGGAACGT TATGAATGGA ACAAGTTGCA AAATGTAAAG 
TCAATAGTAC CCATGGTTCA TCTTTCCTGG AATATGGCAC GAAATATCAA GGTTTCAGAT 
CCAAAGCTTT TTGAAATGAT TAAGTATTGT CTTCTGAGAA CGCTGAAGCA ATGTCAGACA 
TTGAGGGAAG CTCTAATTGC TGCAGGAAAA GAGATCATAT GGCACGGGCG GACAAAAGAA 
GAACCAGCTC ATTATTGTAG TATTTGTGAG GTGGAGGTTT TTGATCTGCT TTTTGTCACT 
AATGAGAGTA ATTCTCGAAA AACCTACATA GTACATTGCC AAGATTGTGC ACGAAAAACA 
AGTGGGAATC TGGAAAATTT TGTGGTGCTA GAACAGTACA AAATGGAGGA TCTGATGCAA 
GTCTATGACC AATTTACATT AGTAAGTGAA ATCAACATGC TCCTCCATTA CCATCCGCCT 
CATCTTGATA TTGTTCCATG GACATTAAAC ATGAGACCTT TTCTGCTATT CAGAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006527662.1
CDS	643..4782
Translation	MKSCGVSLATAAAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARMKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATILQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKNCSNTSGLAARIKYLQNTSDNWSGGNAPPPVEQQTHSWCLTPQKLQHLEQLRANRNNLNPAQKLMLEQLESQFVLMQQHQMRQTGVAQVRPTGILNGPTVDSSLPTNSVSGQQPQLPLTRMPSVSQPGVHTACPRQTLANGPFSAGHVPCSTSRTLGSTDTVLIGNNHVTGSGSNGNVPYLQRNAPTLPHNRTNLTSSTEEPWKNQLSNSTQGLHKGPSSHLAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNTLTVPETSRQTGETPNSTASVEGLPNHVHQVMADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGPGTCDKVNNIHPTVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPIKTDLLLVSHRPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGKRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQNVKSIVPMVHLSWNMARNIKVSDPKLFEMIKYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLVSEINMLLHYHPPHLDIVPWTLNMRPFLLFRK

Target ORF information:

RefSeq Version	XM_006527599.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus lysine (K)-specific demethylase 6A (Kdm6a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527599.3

ORF Insert Sequence:

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCCG CCGCCGCCGC CGCCGCTTTC 
GGTGATGAGG AAAAGAAAAT GGCGGCGGGA AAAGCGAGCG GCGAGAGCGA GGAGGCGTCC 
CCCAGCCTGA CAGCGGAGGA GAGGGAGGCG CTCGGCGGAC TGGACAGCCG CCTTTTCGGG 
TTCGTGAGGT TTCATGAAGA TGGCGCCAGG ATGAAGGCCC TGCTGGGCAA GGCTGTTCGC 
TGCTACGAAT CTCTAATCTT AAAAGCTGAA GGGAAAGTGG AGTCTGATTT CTTTTGTCAA 
TTAGGTCACT TCAACCTCTT ATTGGAAGAT TATCCAAAAG CATTATCTGC ATACCAGAGG 
TACTACAGTT TACAGTCTGA TTACTGGAAG AATGCTGCCT TTTTATATGG TCTTGGTTTG 
GTCTACTTCC ATTACAATGC ATTTCAGTGG GCTATTAAAG CATTTCAGGA GGTGCTTTAT 
GTCGATCCCA GCTTTTGTCG AGCCAAGGAA ATTCATTTAC GACTTGGGCT TATGTTCAAA 
GTGAACACAG ACTATGAGTC TAGTTTAAAG CATTTTCAGT TAGCTTTGGT TGACTGTAAT 
CCCTGCACTT TGTCCAATGC TGAAATTCAG TTTCACATTG CCCACTTATA TGAAACCCAG 
AGGAAGTATC ATTCTGCAAA AGAAGCTTAT GAGCAACTTT TGCAGACAGA AAACCTTTCT 
GCACAAGTAA AAGCAACTAT TTTACAACAA TTAGGTTGGA TGCATCACAC TGTGGATCTC 
CTGGGAGATA AGGCCACCAA GGAAAGTTAT GCTATTCAGT ATCTCCAGAA GTCCTTGGAA 
GCAGATCCAA ATTCTGGCCA GTCCTGGTAT TTCCTTGGAA GGTGCTATTC AAGTATTGGG 
AAAGTTCAGG ATGCCTTTAT ATCTTACAGG CAATCTATTG ATAAATCAGA AGCAAGTGCA 
GATACATGGT GTTCAATAGG TGTGCTCTAT CAACAGCAAA ATCAGCCTAT GGATGCTTTG 
CAAGCTTATA TTTGTGCTGT ACAATTGGAC CACGGTCATG CTGCAGCCTG GATGGATCTA 
GGCACTCTCT ATGAATCCTG CAACCAACCT CAGGATGCTA TTAAATGCTA TTTAAATGCA 
ACTAGAAGCA AAAATTGTAG TAATACCTCT GGACTTGCAG CACGAATTAA GTATTTACAG 
AATACTTCTG ATAATTGGAG TGGTGGCAAT GCACCACCTC CAGTAGAACA ACAAACTCAT 
TCATGGTGTT TGACACCACA GAAATTACAG CACTTGGAAC AGCTCCGAGC AAACAGAAAT 
AATTTAAATC CAGCACAGAA ACTAATGCTG GAACAGCTGG AAAGTCAGTT TGTCTTAATG 
CAGCAACACC AAATGAGACA AACAGGAGTT GCACAGGTAC GGCCTACTGG AATTCTTAAT 
GGGCCAACAG TTGACTCATC ACTGCCTACA AACTCAGTTT CTGGCCAGCA GCCACAGCTT 
CCTCTGACCA GAATGCCTAG TGTCTCTCAG CCTGGAGTCC ACACTGCCTG TCCTAGGCAG 
ACTTTGGCCA ATGGACCCTT TTCTGCAGGC CATGTTCCCT GTAGCACATC AAGAACACTG 
GGAAGTACAG ACACTGTTTT GATAGGCAAT AATCATGTAA CAGGAAGTGG AAGTAATGGA 
AACGTGCCTT ACCTGCAGCG AAACGCACCC ACTCTACCTC ATAACCGCAC AAACCTGACC 
AGCAGCACAG AGGAGCCGTG GAAAAACCAA CTATCTAACT CCACTCAGGG GCTTCACAAA 
GGTCCGAGTT CACATTTGGC AGGTCCTAAT GGTGAACGAC CTCTATCTTC CACTGGGCCT 
TCCCAGCATC TCCAGGCAGC TGGCTCTGGT ATTCAGAATC AGAATGGACA TCCCACCCTG 
CCTAGCAATT CAGTAACACA GGGGGCTGCT CTCAATCACC TCTCCTCTCA CACTGCTACC 
TCAGGTGGAC AACAAGGCAT TACCTTAACC AAAGAGAGCA AGCCTTCAGG AAACACATTG 
ACGGTGCCTG AAACAAGCAG GCAAACTGGA GAGACACCTA ACAGCACTGC CAGTGTTGAG 
GGACTTCCTA ATCATGTCCA TCAGGTGATG GCAGATGCTG TTTGCAGTCC TAGCCATGGA 
GATTCTAAGT CACCAGGTTT ACTAAGTTCA GACAATCCTC AGCTCTCTGC CTTGTTGATG 
GGAAAAGCTA ATAACAATGT GGGTCCTGGA ACCTGTGACA AAGTCAATAA CATCCACCCA 
ACTGTCCATA CAAAGACTGA TAATTCTGTT GCCTCTTCAC CATCTTCAGC CATTTCCACA 
GCAACACCTT CTCCTAAGTC CACTGAACAG ACAACCACAA ACAGTGTTAC CAGCCTTAAC 
AGCCCTCACA GTGGGCTGCA CACAATTAAT GGAGAAGGAA TGGAAGAATC TCAGAGCCCC 
ATTAAAACAG ATCTGCTTCT AGTTAGCCAC AGACCTAGTC CTCAGATCAT ACCATCAATG 
TCTGTGTCCA TATATCCCAG CTCAGCAGAA GTTCTGAAAG CTTGCAGGAA TCTAGGTAAA 
AACGGCCTGT CTAATAGTAG CATTCTGTTG GATAAATGTC CGCCTCCAAG ACCACCATCC 
TCACCATACC CTCCCTTGCC AAAGGACAAG TTGAATCCAC CTACACCTAG TATTTATTTG 
GAAAATAAAC GTGATGCTTT CTTTCCTCCA TTACATCAAT TTTGTACAAA CCCAAACAAC 
CCTGTTACAG TAATACGTGG CCTTGCTGGA GCTCTTAAAT TAGACTTGGG ACTTTTCTCT 
ACTAAAACTT TGGTGGAAGC TAACAATGAA CATATGGTAG AAGTGAGGAC ACAGTTGTTA 
CAACCAGCAG ATGAAAATTG GGACCCTACT GGAACCAAGA AAATCTGGCA CTGTGAAAGT 
AATAGATCTC ATACTACAAT TGCTAAATAT GCTCAGTACC AGGCCTCCTC ATTCCAAGAA 
TCATTGAGAG AAGAAAATGA GAAAAGAAGT CACCATAAAG ACCACTCAGA CAGTGAATCT 
ACATCATCAG ATAATTCTGG GAAAAGAAGA AAAGGACCCT TTAAAACCAT TAAGTTTGGG 
ACCAACATTG ACCTGTCTGA TGACAAAAAG TGGAAGTTAC AGCTACATGA GCTGACTAAA 
CTTCCTGCCT TCGTGAGAGT TGTATCTGCA GGAAATCTTT TAAGCCACGT TGGTCATACT 
ATACTGGGCA TGAACACAGT TCAACTATAC ATGAAAGTTC CAGGAAGCAG AACACCAGGT 
CATCAAGAAA ATAACAACTT CTGTTCAGTT AATATAAATA TTGGCCCAGG TGACTGTGAA 
TGGTTTGTTG TTCCTGAAGG CTACTGGGGT GTTTTGAATG ACTTCTGTGA AAAAAATAAT 
TTGAATTTCT TAATGGGTTC TTGGTGGCCC AACCTTGAAG ATCTATATGA AGCAAATGTT 
CCAGTGTATA GGTTTATTCA GCGACCTGGA GATCTGGTCT GGATAAATGC TGGCACTGTT 
CATTGGGTTC AAGCTATTGG CTGGTGCAAC AATATTGCTT GGAATGTTGG TCCACTTACA 
GCCTGTCAGT ATAAGTTAGC AGTGGAACGT TATGAATGGA ACAAGTTGCA AAATGTAAAG 
TCAATAGTAC CCATGGTTCA TCTTTCCTGG AATATGGCAC GAAATATCAA GGTTTCAGAT 
CCAAAGCTTT TTGAAATGAT TAAGTATTGT CTTCTGAGAA CGCTGAAGCA ATGTCAGACA 
TTGAGGGAAG CTCTAATTGC TGCAGGAAAA GAGATCATAT GGCACGGGCG GACAAAAGAA 
GAACCAGCTC ATTATTGTAG TATTTGTGAG GTGGAGGTTT TTGATCTGCT TTTTGTCACT 
AATGAGAGTA ATTCTCGAAA AACCTACATA GTACATTGCC AAGATTGTGC ACGAAAAACA 
AGTGGGAATC TGGAAAATTT TGTGGTGCTA GAACAGTACA AAATGGAGGA TCTGATGCAA 
GTCTATGACC AATTTACATT AGTAAGTGAA ATCAACATGC TCCTCCATTA CCATCCGCCT 
CATCTTGATA TTGTTCCATG GACATTAAAC ATGAGACCTT TTCTGCTATT CAGAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kdm6a ( XM_006527600.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527663.1 Mus musculus lysine (K)-specific demethylase 6A (Kdm6a), transcript variant X7, mRNA.

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CloneID	OMu55897
Clone ID Related Accession (Same CDS sequence)	XM_006527600.3 , XM_006527600.4
Accession Version	XM_006527600.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	lysine-specific demethylase 6A isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006527600.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCCG CCGCCGCCGC CGCCGCTTTC 
GGTGATGAGG AAAAGAAAAT GGCGGCGGGA AAAGCGAGCG GCGAGAGCGA GGAGGCGTCC 
CCCAGCCTGA CAGCGGAGGA GAGGGAGGCG CTCGGCGGAC TGGACAGCCG CCTTTTCGGG 
TTCGTGAGGT TTCATGAAGA TGGCGCCAGG ATGAAGGCCC TGCTGGGCAA GGCTGTTCGC 
TGCTACGAAT CTCTAATCTT AAAAGCTGAA GGGAAAGTGG AGTCTGATTT CTTTTGTCAA 
TTAGGTCACT TCAACCTCTT ATTGGAAGAT TATCCAAAAG CATTATCTGC ATACCAGAGG 
TACTACAGTT TACAGTCTGA TTACTGGAAG AATGCTGCCT TTTTATATGG TCTTGGTTTG 
GTCTACTTCC ATTACAATGC ATTTCAGTGG GCTATTAAAG CATTTCAGGA GGTGCTTTAT 
GTCGATCCCA GCTTTTGTCG AGCCAAGGAA ATTCATTTAC GACTTGGGCT TATGTTCAAA 
GTGAACACAG ACTATGAGTC TAGTTTAAAG CATTTTCAGT TAGCTTTGGT TGACTGTAAT 
CCCTGCACTT TGTCCAATGC TGAAATTCAG TTTCACATTG CCCACTTATA TGAAACCCAG 
AGGAAGTATC ATTCTGCAAA AGAAGCTTAT GAGCAACTTT TGCAGACAGA AAACCTTTCT 
GCACAAGTAA AAGCAACTAT TTTACAACAA TTAGGTTGGA TGCATCACAC TGTGGATCTC 
CTGGGAGATA AGGCCACCAA GGAAAGTTAT GCTATTCAGT ATCTCCAGAA GTCCTTGGAA 
GCAGATCCAA ATTCTGGCCA GTCCTGGTAT TTCCTTGGAA GGTGCTATTC AAGTATTGGG 
AAAGTTCAGG ATGCCTTTAT ATCTTACAGG CAATCTATTG ATAAATCAGA AGCAAGTGCA 
GATACATGGT GTTCAATAGG TGTGCTCTAT CAACAGCAAA ATCAGCCTAT GGATGCTTTG 
CAAGCTTATA TTTGTGCTGT ACAATTGGAC CACGGTCATG CTGCAGCCTG GATGGATCTA 
GGCACTCTCT ATGAATCCTG CAACCAACCT CAGGATGCTA TTAAATGCTA TTTAAATGCA 
ACTAGAAGCA AAAATTGTAG TAATACCTCT GGACTTGCAG CACGAATTAA GTATTTACAG 
AATACTTCTG ATAATTGGAG TGGTGGCAAT GCACCACCTC CAGTAGAACA ACAAACTCAT 
TCATGGTGTT TGACACCACA GAAATTACAG CACTTGGAAC AGCTCCGAGC AAACAGAAAT 
AATTTAAATC CAGCACAGAA ACTAATGCTG GAACAGCTGG AAAGTCAGTT TGTCTTAATG 
CAGCAACACC AAATGAGACA AACAGGAGTT GCACAGGTAC GGCCTACTGG AATTCTTAAT 
GGGCCAACAG TTGACTCATC ACTGCCTACA AACTCAGTTT CTGGCCAGCA GCCACAGCTT 
CCTCTGACCA GAATGCCTAG TGTCTCTCAG CCTGGAGTCC ACACTGCCTG TCCTAGGCAG 
ACTTTGGCCA ATGGACCCTT TTCTGCAGGC CATGTTCCCT GTAGCACATC AAGAACACTG 
GGAAGTACAG ACACTGTTTT GATAGGCAAT AATCATGTAA CAGGAAGTGG AAGTAATGGA 
AACGTGCCTT ACCTGCAGCG AAACGCACCC ACTCTACCTC ATAACCGCAC AAACCTGACC 
AGCAGCACAG AGGAGCCGTG GAAAAACCAA CTATCTAACT CCACTCAGGG GCTTCACAAA 
GGTCCGAGTT CACATTTGGC AGGTCCTAAT GGTGAACGAC CTCTATCTTC CACTGGGCCT 
TCCCAGCATC TCCAGGCAGC TGGCTCTGGT ATTCAGAATC AGAATGGACA TCCCACCCTG 
CCTAGCAATT CAGTAACACA GGGGGCTGCT CTCAATCACC TCTCCTCTCA CACTGCTACC 
TCAGGTGGAC AACAAGGCAT TACCTTAACC AAAGAGAGCA AGCCTTCAGG AAACACATTG 
ACGGTGCCTG AAACAAGCAG GCAAACTGGA GAGACACCTA ACAGCACTGC CAGTGTTGAG 
GGACTTCCTA ATCATGTCCA TCAGGTGATG GCAGATGCTG TTTGCAGTCC TAGCCATGGA 
GATTCTAAGT CACCAGGTTT ACTAAGTTCA GACAATCCTC AGCTCTCTGC CTTGTTGATG 
GGAAAAGCTA ATAACAATGT GGGTCCTGGA ACCTGTGACA AAGTCAATAA CATCCACCCA 
ACTGTCCATA CAAAGACTGA TAATTCTGTT GCCTCTTCAC CATCTTCAGC CATTTCCACA 
GCAACACCTT CTCCTAAGTC CACTGAACAG ACAACCACAA ACAGTGTTAC CAGCCTTAAC 
AGCCCTCACA GTGGGCTGCA CACAATTAAT GGAGAAGGAA TGGAAGAATC TCAGAGCCCC 
ATTAAAACAG ATCTGCTTCT AGTTAGCCAC AGACCTAGTC CTCAGATCAT ACCATCAATG 
TCTGTGTCCA TATATCCCAG CTCAGCAGAA GTTCTGAAAG CTTGCAGGAA TCTAGGTAAA 
AACGGCCTGT CTAATAGTAG CATTCTGTTG GATAAATGTC CGCCTCCAAG ACCACCATCC 
TCACCATACC CTCCCTTGCC AAAGGACAAG TTGAATCCAC CTACACCTAG TATTTATTTG 
GAAAATAAAC GTGATGCTTT CTTTCCTCCA TTACATCAAT TTTGTACAAA CCCAAACAAC 
CCTGTTACAG TAATACGTGG CCTTGCTGGA GCTCTTAAAT TAGACTTGGG ACTTTTCTCT 
ACTAAAACTT TGGTGGAAGC TAACAATGAA CATATGGTAG AAGTGAGGAC ACAGTTGTTA 
CAACCAGCAG ATGAAAATTG GGACCCTACT GGAACCAAGA AAATCTGGCA CTGTGAAAGT 
AATAGATCTC ATACTACAAT TGCTAAATAT GCTCAGTACC AGGCCTCCTC ATTCCAAGAA 
TCATTGAGAG AAGAAAATGA GAAAAGAAGT CACCATAAAG ACCACTCAGA CAGTGAATCT 
ACATCATCAG ATAATTCTGG GAAAAGAAGA AAAGGACCCT TTAAAACCAT TAAGTTTGGG 
ACCAACATTG ACCTGTCTGA TGACAAAAAG TGGAAGTTAC AGCTACATGA GCTGACTAAA 
CTTCCTGCCT TCGTGAGAGT TGTATCTGCA GGAAATCTTT TAAGCCACGT TGGTCATACT 
ATACTGGGCA TGAACACAGT TCAACTATAC ATGAAAGTTC CAGGAAGCAG AACACCAGGT 
CATCAAGAAA ATAACAACTT CTGTTCAGTT AATATAAATA TTGGCCCAGG TGACTGTGAA 
TGGTTTGTTG TTCCTGAAGG CTACTGGGGT GTTTTGAATG ACTTCTGTGA AAAAAATAAT 
TTGAATTTCT TAATGGGTTC TTGGTGGCCC AACCTTGAAG ATCTATATGA AGCAAATGTT 
CCAGTGTATA GGTTTATTCA GCGACCTGGA GATCTGGTCT GGATAAATGC TGGCACTGTT 
CATTGGGTTC AAGCTATTGG CTGGTGCAAC AATATTGCTT GGAATGTTGG TCCACTTACA 
GCCTGTCAGT ATAAGTTAGC AGTGGAACGT TATGAATGGA ACAAGTTGCA AAATGTAAAG 
TCAATAGTAC CCATGGTTCA TCTTTCCTGG AATATGGCAC GAAATATCAA GGTTTCAGAT 
CCAAAGCTTT TTGAAATGAT TAAGTATTGT CTTCTGAGAA CGCTGAAGCA ATGTCAGACA 
TTGAGGGAAG CTCTAATTGC TGCAGGAAAA GAGATCATAT GGCACGGGCG GACAAAAGAA 
GAACCAGCTC ATTATTGTAG TATTTGTGAG GTGGAGGTTT TTGATCTGCT TTTTGTCACT 
AATGAGAGTA ATTCTCGAAA AACCTACATA GTACATTGCC AAGATTGTGC ACGAAAAACA 
AGTGGGAATC TGGAAAATTT TGTGGTGCTA GAACAGTACA AAATGGAGGA TCTGATGCAA 
GTCTATGACC AATTTACATT AGCTCCTCCA TTACCATCCG CCTCATCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006527663.1
CDS	643..4713
Translation	MKSCGVSLATAAAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARMKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATILQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKNCSNTSGLAARIKYLQNTSDNWSGGNAPPPVEQQTHSWCLTPQKLQHLEQLRANRNNLNPAQKLMLEQLESQFVLMQQHQMRQTGVAQVRPTGILNGPTVDSSLPTNSVSGQQPQLPLTRMPSVSQPGVHTACPRQTLANGPFSAGHVPCSTSRTLGSTDTVLIGNNHVTGSGSNGNVPYLQRNAPTLPHNRTNLTSSTEEPWKNQLSNSTQGLHKGPSSHLAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNTLTVPETSRQTGETPNSTASVEGLPNHVHQVMADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGPGTCDKVNNIHPTVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPIKTDLLLVSHRPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGKRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQNVKSIVPMVHLSWNMARNIKVSDPKLFEMIKYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLAPPLPSASS

Target ORF information:

RefSeq Version	XM_006527600.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus lysine (K)-specific demethylase 6A (Kdm6a), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527600.3

ORF Insert Sequence:

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCCG CCGCCGCCGC CGCCGCTTTC 
GGTGATGAGG AAAAGAAAAT GGCGGCGGGA AAAGCGAGCG GCGAGAGCGA GGAGGCGTCC 
CCCAGCCTGA CAGCGGAGGA GAGGGAGGCG CTCGGCGGAC TGGACAGCCG CCTTTTCGGG 
TTCGTGAGGT TTCATGAAGA TGGCGCCAGG ATGAAGGCCC TGCTGGGCAA GGCTGTTCGC 
TGCTACGAAT CTCTAATCTT AAAAGCTGAA GGGAAAGTGG AGTCTGATTT CTTTTGTCAA 
TTAGGTCACT TCAACCTCTT ATTGGAAGAT TATCCAAAAG CATTATCTGC ATACCAGAGG 
TACTACAGTT TACAGTCTGA TTACTGGAAG AATGCTGCCT TTTTATATGG TCTTGGTTTG 
GTCTACTTCC ATTACAATGC ATTTCAGTGG GCTATTAAAG CATTTCAGGA GGTGCTTTAT 
GTCGATCCCA GCTTTTGTCG AGCCAAGGAA ATTCATTTAC GACTTGGGCT TATGTTCAAA 
GTGAACACAG ACTATGAGTC TAGTTTAAAG CATTTTCAGT TAGCTTTGGT TGACTGTAAT 
CCCTGCACTT TGTCCAATGC TGAAATTCAG TTTCACATTG CCCACTTATA TGAAACCCAG 
AGGAAGTATC ATTCTGCAAA AGAAGCTTAT GAGCAACTTT TGCAGACAGA AAACCTTTCT 
GCACAAGTAA AAGCAACTAT TTTACAACAA TTAGGTTGGA TGCATCACAC TGTGGATCTC 
CTGGGAGATA AGGCCACCAA GGAAAGTTAT GCTATTCAGT ATCTCCAGAA GTCCTTGGAA 
GCAGATCCAA ATTCTGGCCA GTCCTGGTAT TTCCTTGGAA GGTGCTATTC AAGTATTGGG 
AAAGTTCAGG ATGCCTTTAT ATCTTACAGG CAATCTATTG ATAAATCAGA AGCAAGTGCA 
GATACATGGT GTTCAATAGG TGTGCTCTAT CAACAGCAAA ATCAGCCTAT GGATGCTTTG 
CAAGCTTATA TTTGTGCTGT ACAATTGGAC CACGGTCATG CTGCAGCCTG GATGGATCTA 
GGCACTCTCT ATGAATCCTG CAACCAACCT CAGGATGCTA TTAAATGCTA TTTAAATGCA 
ACTAGAAGCA AAAATTGTAG TAATACCTCT GGACTTGCAG CACGAATTAA GTATTTACAG 
AATACTTCTG ATAATTGGAG TGGTGGCAAT GCACCACCTC CAGTAGAACA ACAAACTCAT 
TCATGGTGTT TGACACCACA GAAATTACAG CACTTGGAAC AGCTCCGAGC AAACAGAAAT 
AATTTAAATC CAGCACAGAA ACTAATGCTG GAACAGCTGG AAAGTCAGTT TGTCTTAATG 
CAGCAACACC AAATGAGACA AACAGGAGTT GCACAGGTAC GGCCTACTGG AATTCTTAAT 
GGGCCAACAG TTGACTCATC ACTGCCTACA AACTCAGTTT CTGGCCAGCA GCCACAGCTT 
CCTCTGACCA GAATGCCTAG TGTCTCTCAG CCTGGAGTCC ACACTGCCTG TCCTAGGCAG 
ACTTTGGCCA ATGGACCCTT TTCTGCAGGC CATGTTCCCT GTAGCACATC AAGAACACTG 
GGAAGTACAG ACACTGTTTT GATAGGCAAT AATCATGTAA CAGGAAGTGG AAGTAATGGA 
AACGTGCCTT ACCTGCAGCG AAACGCACCC ACTCTACCTC ATAACCGCAC AAACCTGACC 
AGCAGCACAG AGGAGCCGTG GAAAAACCAA CTATCTAACT CCACTCAGGG GCTTCACAAA 
GGTCCGAGTT CACATTTGGC AGGTCCTAAT GGTGAACGAC CTCTATCTTC CACTGGGCCT 
TCCCAGCATC TCCAGGCAGC TGGCTCTGGT ATTCAGAATC AGAATGGACA TCCCACCCTG 
CCTAGCAATT CAGTAACACA GGGGGCTGCT CTCAATCACC TCTCCTCTCA CACTGCTACC 
TCAGGTGGAC AACAAGGCAT TACCTTAACC AAAGAGAGCA AGCCTTCAGG AAACACATTG 
ACGGTGCCTG AAACAAGCAG GCAAACTGGA GAGACACCTA ACAGCACTGC CAGTGTTGAG 
GGACTTCCTA ATCATGTCCA TCAGGTGATG GCAGATGCTG TTTGCAGTCC TAGCCATGGA 
GATTCTAAGT CACCAGGTTT ACTAAGTTCA GACAATCCTC AGCTCTCTGC CTTGTTGATG 
GGAAAAGCTA ATAACAATGT GGGTCCTGGA ACCTGTGACA AAGTCAATAA CATCCACCCA 
ACTGTCCATA CAAAGACTGA TAATTCTGTT GCCTCTTCAC CATCTTCAGC CATTTCCACA 
GCAACACCTT CTCCTAAGTC CACTGAACAG ACAACCACAA ACAGTGTTAC CAGCCTTAAC 
AGCCCTCACA GTGGGCTGCA CACAATTAAT GGAGAAGGAA TGGAAGAATC TCAGAGCCCC 
ATTAAAACAG ATCTGCTTCT AGTTAGCCAC AGACCTAGTC CTCAGATCAT ACCATCAATG 
TCTGTGTCCA TATATCCCAG CTCAGCAGAA GTTCTGAAAG CTTGCAGGAA TCTAGGTAAA 
AACGGCCTGT CTAATAGTAG CATTCTGTTG GATAAATGTC CGCCTCCAAG ACCACCATCC 
TCACCATACC CTCCCTTGCC AAAGGACAAG TTGAATCCAC CTACACCTAG TATTTATTTG 
GAAAATAAAC GTGATGCTTT CTTTCCTCCA TTACATCAAT TTTGTACAAA CCCAAACAAC 
CCTGTTACAG TAATACGTGG CCTTGCTGGA GCTCTTAAAT TAGACTTGGG ACTTTTCTCT 
ACTAAAACTT TGGTGGAAGC TAACAATGAA CATATGGTAG AAGTGAGGAC ACAGTTGTTA 
CAACCAGCAG ATGAAAATTG GGACCCTACT GGAACCAAGA AAATCTGGCA CTGTGAAAGT 
AATAGATCTC ATACTACAAT TGCTAAATAT GCTCAGTACC AGGCCTCCTC ATTCCAAGAA 
TCATTGAGAG AAGAAAATGA GAAAAGAAGT CACCATAAAG ACCACTCAGA CAGTGAATCT 
ACATCATCAG ATAATTCTGG GAAAAGAAGA AAAGGACCCT TTAAAACCAT TAAGTTTGGG 
ACCAACATTG ACCTGTCTGA TGACAAAAAG TGGAAGTTAC AGCTACATGA GCTGACTAAA 
CTTCCTGCCT TCGTGAGAGT TGTATCTGCA GGAAATCTTT TAAGCCACGT TGGTCATACT 
ATACTGGGCA TGAACACAGT TCAACTATAC ATGAAAGTTC CAGGAAGCAG AACACCAGGT 
CATCAAGAAA ATAACAACTT CTGTTCAGTT AATATAAATA TTGGCCCAGG TGACTGTGAA 
TGGTTTGTTG TTCCTGAAGG CTACTGGGGT GTTTTGAATG ACTTCTGTGA AAAAAATAAT 
TTGAATTTCT TAATGGGTTC TTGGTGGCCC AACCTTGAAG ATCTATATGA AGCAAATGTT 
CCAGTGTATA GGTTTATTCA GCGACCTGGA GATCTGGTCT GGATAAATGC TGGCACTGTT 
CATTGGGTTC AAGCTATTGG CTGGTGCAAC AATATTGCTT GGAATGTTGG TCCACTTACA 
GCCTGTCAGT ATAAGTTAGC AGTGGAACGT TATGAATGGA ACAAGTTGCA AAATGTAAAG 
TCAATAGTAC CCATGGTTCA TCTTTCCTGG AATATGGCAC GAAATATCAA GGTTTCAGAT 
CCAAAGCTTT TTGAAATGAT TAAGTATTGT CTTCTGAGAA CGCTGAAGCA ATGTCAGACA 
TTGAGGGAAG CTCTAATTGC TGCAGGAAAA GAGATCATAT GGCACGGGCG GACAAAAGAA 
GAACCAGCTC ATTATTGTAG TATTTGTGAG GTGGAGGTTT TTGATCTGCT TTTTGTCACT 
AATGAGAGTA ATTCTCGAAA AACCTACATA GTACATTGCC AAGATTGTGC ACGAAAAACA 
AGTGGGAATC TGGAAAATTT TGTGGTGCTA GAACAGTACA AAATGGAGGA TCTGATGCAA 
GTCTATGACC AATTTACATT AGCTCCTCCA TTACCATCCG CCTCATCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Kdm6a ( XM_017318461.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173950.1 Mus musculus lysine (K)-specific demethylase 6A (Kdm6a), transcript variant X3, mRNA.

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CloneID	OMu83109
Clone ID Related Accession (Same CDS sequence)	XM_017318461.1 , XM_017318461.2
Accession Version	XM_017318461.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4332bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	lysine-specific demethylase 6A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCCG CCGCCGCCGC CGCCGCTTTC 
GGTGATGAGG AAAAGAAAAT GGCGGCGGGA AAAGCGAGCG GCGAGAGCGA GGAGGCGTCC 
CCCAGCCTGA CAGCGGAGGA GAGGGAGGCG CTCGGCGGAC TGGACAGCCG CCTTTTCGGG 
TTCGTGAGGT TTCATGAAGA TGGCGCCAGG ATGAAGGCCC TGCTGGGCAA GGCTGTTCGC 
TGCTACGAAT CTCTAATCTT AAAAGCTGAA GGGAAAGTGG AGTCTGATTT CTTTTGTCAA 
TTAGGTCACT TCAACCTCTT ATTGGAAGAT TATCCAAAAG CATTATCTGC ATACCAGAGG 
TACTACAGTT TACAGTCTGA TTACTGGAAG AATGCTGCCT TTTTATATGG TCTTGGTTTG 
GTCTACTTCC ATTACAATGC ATTTCAGTGG GCTATTAAAG CATTTCAGGA GGTGCTTTAT 
GTCGATCCCA GCTTTTGTCG AGCCAAGGAA ATTCATTTAC GACTTGGGCT TATGTTCAAA 
GTGAACACAG ACTATGAGTC TAGTTTAAAG CATTTTCAGT TAGCTTTGGT TGACTGTAAT 
CCCTGCACTT TGTCCAATGC TGAAATTCAG TTTCACATTG CCCACTTATA TGAAACCCAG 
AGGAAGTATC ATTCTGCAAA AGAAGCTTAT GAGCAACTTT TGCAGACAGA AAACCTTTCT 
GCACAAGTAA AAGCAACTAT TTTACAACAA TTAGGTTGGA TGCATCACAC TGTGGATCTC 
CTGGGAGATA AGGCCACCAA GGAAAGTTAT GCTATTCAGT ATCTCCAGAA GTCCTTGGAA 
GCAGATCCAA ATTCTGGCCA GTCCTGGTAT TTCCTTGGAA GTGTGCTCTA TCAACAGCAA 
AATCAGCCTA TGGATGCTTT GCAAGCTTAT ATTTGTGCTG TACAATTGGA CCACGGTCAT 
GCTGCAGCCT GGATGGATCT AGGCACTCTC TATGAATCCT GCAACCAACC TCAGGATGCT 
ATTAAATGCT ATTTAAATGC AACTAGAAGC AAAAATTGTA GTAATACCTC TGGACTTGCA 
GCACGAATTA AGTATTTACA GGCTCAGTTG TGTAACCTTC CACAAGGTAG TCTACAGAAT 
AAAACTAAAT TACTTCCTAG TATTGAGGAG GCATGGAGCC TACCAATCCC CGCAGAGCTT 
ACCTCCAGGC AGGGTGCCAT GAACACAGCA CAGCAGGCAT GTAAACCTCA TCATCCAAAT 
ACTGAACCTG TATTAGGCCT CAGTCAAACA CCAATTTCAC AGCAGTCCTT GCCACTACAC 
ATGATTCCTC CTAGCCAAGT AGATGACCTG TCCAGTCCTG CCAAGAGGAA AAGAACATCT 
AGTCCAACAA AGAATACTTC TGATAATTGG AGTGGTGGCA ATGCACCACC TCCAGTAGAA 
CAACAAACTC ATTCATGGTG TTTGACACCA CAGAAATTAC AGCACTTGGA ACAGCTCCGA 
GCAAACAGAA ATAATTTAAA TCCAGCACAG AAACTAATGC TGGAACAGCT GGAAAGTCAG 
TTTGTCTTAA TGCAGCAACA CCAAATGAGA CAAACAGGAG TTGCACAGGT ACGGCCTACT 
GGAATTCTTA ATGGGCCAAC AGTTGACTCA TCACTGCCTA CAAACTCAGT TTCTGGCCAG 
CAGCCACAGC TTCCTCTGAC CAGAATGCCT AGTGTCTCTC AGCCTGGAGT CCACACTGCC 
TGTCCTAGGC AGACTTTGGC CAATGGACCC TTTTCTGCAG GCCATGTTCC CTGTAGCACA 
TCAAGAACAC TGGGAAGTAC AGACACTGTT TTGATAGGCA ATAATCATGT AACAGGAAGT 
GGAAGTAATG GAAACGTGCC TTACCTGCAG CGAAACGCAC CCACTCTACC TCATAACCGC 
ACAAACCTGA CCAGCAGCAC AGAGGAGCCG TGGAAAAACC AACTATCTAA CTCCACTCAG 
GGGCTTCACA AAGGTCCGAG TTCACATTTG GCAGGTCCTA ATGGTGAACG ACCTCTATCT 
TCCACTGGGC CTTCCCAGCA TCTCCAGGCA GCTGGCTCTG GTATTCAGAA TCAGAATGGA 
CATCCCACCC TGCCTAGCAA TTCAGTAACA CAGGGGGCTG CTCTCAATCA CCTCTCCTCT 
CACACTGCTA CCTCAGGTGG ACAACAAGGC ATTACCTTAA CCAAAGAGAG CAAGCCTTCA 
GGAAACACAT TGACGGTGCC TGAAACAAGC AGGCAAACTG GAGAGACACC TAACAGCACT 
GCCAGTGTTG AGGGACTTCC TAATCATGTC CATCAGGTGA TGGCAGATGC TGTTTGCAGT 
CCTAGCCATG GAGATTCTAA GTCACCAGGT TTACTAAGTT CAGACAATCC TCAGCTCTCT 
GCCTTGTTGA TGGGAAAAGC TAATAACAAT GTGGGTCCTG GAACCTGTGA CAAAGTCAAT 
AACATCCACC CAACTGTCCA TACAAAGACT GATAATTCTG TTGCCTCTTC ACCATCTTCA 
GCCATTTCCA CAGCAACACC TTCTCCTAAG TCCACTGAAC AGACAACCAC AAACAGTGTT 
ACCAGCCTTA ACAGCCCTCA CAGTGGGCTG CACACAATTA ATGGAGAAGG AATGGAAGAA 
TCTCAGAGCC CCATTAAAAC AGATCTGCTT CTAGTTAGCC ACAGACCTAG TCCTCAGATC 
ATACCATCAA TGTCTGTGTC CATATATCCC AGCTCAGCAG AAGTTCTGAA AGCTTGCAGG 
AATCTAGGTA AAAACGGCCT GTCTAATAGT AGCATTCTGT TGGATAAATG TCCGCCTCCA 
AGACCACCAT CCTCACCATA CCCTCCCTTG CCAAAGGACA AGTTGAATCC ACCTACACCT 
AGTATTTATT TGGAAAATAA ACGTGATGCT TTCTTTCCTC CATTACATCA ATTTTGTACA 
AACCCAAACA ACCCTGTTAC AGTAATACGT GGCCTTGCTG GAGCTCTTAA ATTAGACTTG 
GGACTTTTCT CTACTAAAAC TTTGGTGGAA GCTAACAATG AACATATGGT AGAAGTGAGG 
ACACAGTTGT TACAACCAGC AGATGAAAAT TGGGACCCTA CTGGAACCAA GAAAATCTGG 
CACTGTGAAA GTAATAGATC TCATACTACA ATTGCTAAAT ATGCTCAGTA CCAGGCCTCC 
TCATTCCAAG AATCATTGAG AGAAGAAAAT GAGAAAAGAA GTCACCATAA AGACCACTCA 
GACAGTGAAT CTACATCATC AGATAATTCT GGGAAAAGAA GAAAAGGACC CTTTAAAACC 
ATTAAGTTTG GGACCAACAT TGACCTGTCT GATGACAAAA AGTGGAAGTT ACAGCTACAT 
GAGCTGACTA AACTTCCTGC CTTCGTGAGA GTTGTATCTG CAGGAAATCT TTTAAGCCAC 
GTTGGTCATA CTATACTGGG CATGAACACA GTTCAACTAT ACATGAAAGT TCCAGGAAGC 
AGAACACCAG GTCATCAAGA AAATAACAAC TTCTGTTCAG TTAATATAAA TATTGGCCCA 
GGTGACTGTG AATGGTTTGT TGTTCCTGAA GGCTACTGGG GTGTTTTGAA TGACTTCTGT 
GAAAAAAATA ATTTGAATTT CTTAATGGGT TCTTGGTGGC CCAACCTTGA AGATCTATAT 
GAAGCAAATG TTCCAGTGTA TAGGTTTATT CAGCGACCTG GAGATCTGGT CTGGATAAAT 
GCTGGCACTG TTCATTGGGT TCAAGCTATT GGCTGGTGCA ACAATATTGC TTGGAATGTT 
GGTCCACTTA CAGCCTGTCA GTATAAGTTA GCAGTGGAAC GTTATGAATG GAACAAGTTG 
CAAAATGTAA AGTCAATAGT ACCCATGGTT CATCTTTCCT GGAATATGGC ACGAAATATC 
AAGGTTTCAG ATCCAAAGCT TTTTGAAATG ATTAAGTATT GTCTTCTGAG AACGCTGAAG 
CAATGTCAGA CATTGAGGGA AGCTCTAATT GCTGCAGGAA AAGAGATCAT ATGGCACGGG 
CGGACAAAAG AAGAACCAGC TCATTATTGT AGTATTTGTG AGGTGGAGGT TTTTGATCTG 
CTTTTTGTCA CTAATGAGAG TAATTCTCGA AAAACCTACA TAGTACATTG CCAAGATTGT 
GCACGAAAAA CAAGTGGGAA TCTGGAAAAT TTTGTGGTGC TAGAACAGTA CAAAATGGAG 
GATCTGATGC AAGTCTATGA CCAATTTACA TTAGTAAGTG AAATCAACAT GCTCCTCCAT 
TACCATCCGC CTCATCTTGA TATTGTTCCA TGGACATTAA ACATGAGACC TTTTCTGCTA 
TTCAGAAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017173950.1
CDS	643..4974
Translation	MKSCGVSLATAAAAAAAAAFGDEEKKMAAGKASGESEEASPSLTAEEREALGGLDSRLFGFVRFHEDGARMKALLGKAVRCYESLILKAEGKVESDFFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFQWAIKAFQEVLYVDPSFCRAKEIHLRLGLMFKVNTDYESSLKHFQLALVDCNPCTLSNAEIQFHIAHLYETQRKYHSAKEAYEQLLQTENLSAQVKATILQQLGWMHHTVDLLGDKATKESYAIQYLQKSLEADPNSGQSWYFLGSVLYQQQNQPMDALQAYICAVQLDHGHAAAWMDLGTLYESCNQPQDAIKCYLNATRSKNCSNTSGLAARIKYLQAQLCNLPQGSLQNKTKLLPSIEEAWSLPIPAELTSRQGAMNTAQQACKPHHPNTEPVLGLSQTPISQQSLPLHMIPPSQVDDLSSPAKRKRTSSPTKNTSDNWSGGNAPPPVEQQTHSWCLTPQKLQHLEQLRANRNNLNPAQKLMLEQLESQFVLMQQHQMRQTGVAQVRPTGILNGPTVDSSLPTNSVSGQQPQLPLTRMPSVSQPGVHTACPRQTLANGPFSAGHVPCSTSRTLGSTDTVLIGNNHVTGSGSNGNVPYLQRNAPTLPHNRTNLTSSTEEPWKNQLSNSTQGLHKGPSSHLAGPNGERPLSSTGPSQHLQAAGSGIQNQNGHPTLPSNSVTQGAALNHLSSHTATSGGQQGITLTKESKPSGNTLTVPETSRQTGETPNSTASVEGLPNHVHQVMADAVCSPSHGDSKSPGLLSSDNPQLSALLMGKANNNVGPGTCDKVNNIHPTVHTKTDNSVASSPSSAISTATPSPKSTEQTTTNSVTSLNSPHSGLHTINGEGMEESQSPIKTDLLLVSHRPSPQIIPSMSVSIYPSSAEVLKACRNLGKNGLSNSSILLDKCPPPRPPSSPYPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNPNNPVTVIRGLAGALKLDLGLFSTKTLVEANNEHMVEVRTQLLQPADENWDPTGTKKIWHCESNRSHTTIAKYAQYQASSFQESLREENEKRSHHKDHSDSESTSSDNSGKRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQNVKSIVPMVHLSWNMARNIKVSDPKLFEMIKYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLVSEINMLLHYHPPHLDIVPWTLNMRPFLLFRK

Target ORF information:

RefSeq Version	XM_017318461.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus lysine (K)-specific demethylase 6A (Kdm6a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017318461.1

ORF Insert Sequence:

ATGAAATCCT GCGGAGTGTC GCTCGCTACC GCCGCCGCCG CCGCCGCCGC CGCCGCTTTC 
GGTGATGAGG AAAAGAAAAT GGCGGCGGGA AAAGCGAGCG GCGAGAGCGA GGAGGCGTCC 
CCCAGCCTGA CAGCGGAGGA GAGGGAGGCG CTCGGCGGAC TGGACAGCCG CCTTTTCGGG 
TTCGTGAGGT TTCATGAAGA TGGCGCCAGG ATGAAGGCCC TGCTGGGCAA GGCTGTTCGC 
TGCTACGAAT CTCTAATCTT AAAAGCTGAA GGGAAAGTGG AGTCTGATTT CTTTTGTCAA 
TTAGGTCACT TCAACCTCTT ATTGGAAGAT TATCCAAAAG CATTATCTGC ATACCAGAGG 
TACTACAGTT TACAGTCTGA TTACTGGAAG AATGCTGCCT TTTTATATGG TCTTGGTTTG 
GTCTACTTCC ATTACAATGC ATTTCAGTGG GCTATTAAAG CATTTCAGGA GGTGCTTTAT 
GTCGATCCCA GCTTTTGTCG AGCCAAGGAA ATTCATTTAC GACTTGGGCT TATGTTCAAA 
GTGAACACAG ACTATGAGTC TAGTTTAAAG CATTTTCAGT TAGCTTTGGT TGACTGTAAT 
CCCTGCACTT TGTCCAATGC TGAAATTCAG TTTCACATTG CCCACTTATA TGAAACCCAG 
AGGAAGTATC ATTCTGCAAA AGAAGCTTAT GAGCAACTTT TGCAGACAGA AAACCTTTCT 
GCACAAGTAA AAGCAACTAT TTTACAACAA TTAGGTTGGA TGCATCACAC TGTGGATCTC 
CTGGGAGATA AGGCCACCAA GGAAAGTTAT GCTATTCAGT ATCTCCAGAA GTCCTTGGAA 
GCAGATCCAA ATTCTGGCCA GTCCTGGTAT TTCCTTGGAA GTGTGCTCTA TCAACAGCAA 
AATCAGCCTA TGGATGCTTT GCAAGCTTAT ATTTGTGCTG TACAATTGGA CCACGGTCAT 
GCTGCAGCCT GGATGGATCT AGGCACTCTC TATGAATCCT GCAACCAACC TCAGGATGCT 
ATTAAATGCT ATTTAAATGC AACTAGAAGC AAAAATTGTA GTAATACCTC TGGACTTGCA 
GCACGAATTA AGTATTTACA GGCTCAGTTG TGTAACCTTC CACAAGGTAG TCTACAGAAT 
AAAACTAAAT TACTTCCTAG TATTGAGGAG GCATGGAGCC TACCAATCCC CGCAGAGCTT 
ACCTCCAGGC AGGGTGCCAT GAACACAGCA CAGCAGGCAT GTAAACCTCA TCATCCAAAT 
ACTGAACCTG TATTAGGCCT CAGTCAAACA CCAATTTCAC AGCAGTCCTT GCCACTACAC 
ATGATTCCTC CTAGCCAAGT AGATGACCTG TCCAGTCCTG CCAAGAGGAA AAGAACATCT 
AGTCCAACAA AGAATACTTC TGATAATTGG AGTGGTGGCA ATGCACCACC TCCAGTAGAA 
CAACAAACTC ATTCATGGTG TTTGACACCA CAGAAATTAC AGCACTTGGA ACAGCTCCGA 
GCAAACAGAA ATAATTTAAA TCCAGCACAG AAACTAATGC TGGAACAGCT GGAAAGTCAG 
TTTGTCTTAA TGCAGCAACA CCAAATGAGA CAAACAGGAG TTGCACAGGT ACGGCCTACT 
GGAATTCTTA ATGGGCCAAC AGTTGACTCA TCACTGCCTA CAAACTCAGT TTCTGGCCAG 
CAGCCACAGC TTCCTCTGAC CAGAATGCCT AGTGTCTCTC AGCCTGGAGT CCACACTGCC 
TGTCCTAGGC AGACTTTGGC CAATGGACCC TTTTCTGCAG GCCATGTTCC CTGTAGCACA 
TCAAGAACAC TGGGAAGTAC AGACACTGTT TTGATAGGCA ATAATCATGT AACAGGAAGT 
GGAAGTAATG GAAACGTGCC TTACCTGCAG CGAAACGCAC CCACTCTACC TCATAACCGC 
ACAAACCTGA CCAGCAGCAC AGAGGAGCCG TGGAAAAACC AACTATCTAA CTCCACTCAG 
GGGCTTCACA AAGGTCCGAG TTCACATTTG GCAGGTCCTA ATGGTGAACG ACCTCTATCT 
TCCACTGGGC CTTCCCAGCA TCTCCAGGCA GCTGGCTCTG GTATTCAGAA TCAGAATGGA 
CATCCCACCC TGCCTAGCAA TTCAGTAACA CAGGGGGCTG CTCTCAATCA CCTCTCCTCT 
CACACTGCTA CCTCAGGTGG ACAACAAGGC ATTACCTTAA CCAAAGAGAG CAAGCCTTCA 
GGAAACACAT TGACGGTGCC TGAAACAAGC AGGCAAACTG GAGAGACACC TAACAGCACT 
GCCAGTGTTG AGGGACTTCC TAATCATGTC CATCAGGTGA TGGCAGATGC TGTTTGCAGT 
CCTAGCCATG GAGATTCTAA GTCACCAGGT TTACTAAGTT CAGACAATCC TCAGCTCTCT 
GCCTTGTTGA TGGGAAAAGC TAATAACAAT GTGGGTCCTG GAACCTGTGA CAAAGTCAAT 
AACATCCACC CAACTGTCCA TACAAAGACT GATAATTCTG TTGCCTCTTC ACCATCTTCA 
GCCATTTCCA CAGCAACACC TTCTCCTAAG TCCACTGAAC AGACAACCAC AAACAGTGTT 
ACCAGCCTTA ACAGCCCTCA CAGTGGGCTG CACACAATTA ATGGAGAAGG AATGGAAGAA 
TCTCAGAGCC CCATTAAAAC AGATCTGCTT CTAGTTAGCC ACAGACCTAG TCCTCAGATC 
ATACCATCAA TGTCTGTGTC CATATATCCC AGCTCAGCAG AAGTTCTGAA AGCTTGCAGG 
AATCTAGGTA AAAACGGCCT GTCTAATAGT AGCATTCTGT TGGATAAATG TCCGCCTCCA 
AGACCACCAT CCTCACCATA CCCTCCCTTG CCAAAGGACA AGTTGAATCC ACCTACACCT 
AGTATTTATT TGGAAAATAA ACGTGATGCT TTCTTTCCTC CATTACATCA ATTTTGTACA 
AACCCAAACA ACCCTGTTAC AGTAATACGT GGCCTTGCTG GAGCTCTTAA ATTAGACTTG 
GGACTTTTCT CTACTAAAAC TTTGGTGGAA GCTAACAATG AACATATGGT AGAAGTGAGG 
ACACAGTTGT TACAACCAGC AGATGAAAAT TGGGACCCTA CTGGAACCAA GAAAATCTGG 
CACTGTGAAA GTAATAGATC TCATACTACA ATTGCTAAAT ATGCTCAGTA CCAGGCCTCC 
TCATTCCAAG AATCATTGAG AGAAGAAAAT GAGAAAAGAA GTCACCATAA AGACCACTCA 
GACAGTGAAT CTACATCATC AGATAATTCT GGGAAAAGAA GAAAAGGACC CTTTAAAACC 
ATTAAGTTTG GGACCAACAT TGACCTGTCT GATGACAAAA AGTGGAAGTT ACAGCTACAT 
GAGCTGACTA AACTTCCTGC CTTCGTGAGA GTTGTATCTG CAGGAAATCT TTTAAGCCAC 
GTTGGTCATA CTATACTGGG CATGAACACA GTTCAACTAT ACATGAAAGT TCCAGGAAGC 
AGAACACCAG GTCATCAAGA AAATAACAAC TTCTGTTCAG TTAATATAAA TATTGGCCCA 
GGTGACTGTG AATGGTTTGT TGTTCCTGAA GGCTACTGGG GTGTTTTGAA TGACTTCTGT 
GAAAAAAATA ATTTGAATTT CTTAATGGGT TCTTGGTGGC CCAACCTTGA AGATCTATAT 
GAAGCAAATG TTCCAGTGTA TAGGTTTATT CAGCGACCTG GAGATCTGGT CTGGATAAAT 
GCTGGCACTG TTCATTGGGT TCAAGCTATT GGCTGGTGCA ACAATATTGC TTGGAATGTT 
GGTCCACTTA CAGCCTGTCA GTATAAGTTA GCAGTGGAAC GTTATGAATG GAACAAGTTG 
CAAAATGTAA AGTCAATAGT ACCCATGGTT CATCTTTCCT GGAATATGGC ACGAAATATC 
AAGGTTTCAG ATCCAAAGCT TTTTGAAATG ATTAAGTATT GTCTTCTGAG AACGCTGAAG 
CAATGTCAGA CATTGAGGGA AGCTCTAATT GCTGCAGGAA AAGAGATCAT ATGGCACGGG 
CGGACAAAAG AAGAACCAGC TCATTATTGT AGTATTTGTG AGGTGGAGGT TTTTGATCTG 
CTTTTTGTCA CTAATGAGAG TAATTCTCGA AAAACCTACA TAGTACATTG CCAAGATTGT 
GCACGAAAAA CAAGTGGGAA TCTGGAAAAT TTTGTGGTGC TAGAACAGTA CAAAATGGAG 
GATCTGATGC AAGTCTATGA CCAATTTACA TTAGTAAGTG AAATCAACAT GCTCCTCCAT 
TACCATCCGC CTCATCTTGA TATTGTTCCA TGGACATTAA ACATGAGACC TTTTCTGCTA 
TTCAGAAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Kdm6a
Entrez Gene ID	22289
Full Name	lysine (K)-specific demethylase 6A
Synonyms	Utx
General protein information	Preferred Names lysine (K)-specific demethylase 6A Names lysine-specific demethylase 6A histone demethylase UTX ubiquitously transcribed TPR protein on the X chromosome ubiquitously transcribed X chromosome tetratricopeptide repeat protein ubiquitously transcribed tetratricopeptide repeat gene, X chromosome
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: X Map Location: X A1.2-A1.3\|X 13.45 cM

Homo sapiens (human)	KDM6A	NP_066963.2
Danio rerio (zebrafish)	kdm6a	XP_697746.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008509	XP_316935.4
Xenopus tropicalis (tropical clawed frog)	kdm6a	NP_001123807.1
Bos taurus (cattle)	KDM6A	NP_001193504.1
Drosophila melanogaster (fruit fly)	Utx	NP_001188773.1
Pan troglodytes (chimpanzee)	KDM6A	XP_003954022.1
Canis lupus familiaris (dog)	KDM6A	NP_001184114.1
Caenorhabditis elegans (roundworm)	utx-1	NP_509450.1
Rattus norvegicus (Norway rat)	Kdm6a	XP_006256701.1
	KDM6A	XP_002806253.1
Mus musculus (house mouse)	Kdm6a	NP_033509.1
Gallus gallus (chicken)	KDM6A	XP_416762.4

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Kdm6a cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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