Usp5 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Usp5
Entrez Gene ID	22225
Full Name	ubiquitin specific peptidase 5 (isopeptidase T)
Synonyms	AA407472,ISOT,ISOT-1,Ucht
General protein information	Preferred Names ubiquitin specific peptidase 5 (isopeptidase T) Names ubiquitin carboxyl-terminal hydrolase 5 deubiquitinating enzyme 5 isopeptidase T ubiquitin carboxy terminal hydrolase T ubiquitin specific protease 5 ubiquitin thioesterase 5 ubiquitin thiolesterase 5 ubiquitin-specific-processing protease 5
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6 F2\|6 59.17 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006505916.3	XP_006505979.1	ubiquitin carboxyl-terminal hydrolase 5 isoform X1
NM_013700.2	NP_038728.1	ubiquitin carboxyl-terminal hydrolase 5 isoform 1
XM_006505916.4	XP_006505979.1	ubiquitin carboxyl-terminal hydrolase 5 isoform X1
XM_030255318.1	XP_030111178.1	ubiquitin carboxyl-terminal hydrolase 5 isoform X2
XM_030255317.1	XP_030111177.1	ubiquitin carboxyl-terminal hydrolase 5 isoform X1
NM_001326594.1	NP_001313523.1	ubiquitin carboxyl-terminal hydrolase 5 isoform 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	USP5	NP_001092006.1
Mus musculus (house mouse)	Usp5	NP_038728.1
Danio rerio (zebrafish)	usp5	NP_999920.2
Drosophila melanogaster (fruit fly)	CG12082	NP_647773.1
Pan troglodytes (chimpanzee)	USP5	XP_508970.4
Bos taurus (cattle)	USP5	NP_001178985.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012248	XP_320294.3
	USP5	XP_001110639.1
Canis lupus familiaris (dog)	USP5	XP_543845.3
Rattus norvegicus (Norway rat)	Usp5	NP_001100089.1
Gallus gallus (chicken)	USP5	XP_003640490.1
Oryza sativa (rice)	Os01g0177200	NP_001042185.1
Arabidopsis thaliana (thale cress)	UBP14	NP_566666.2
Xenopus tropicalis (tropical clawed frog)	usp5	NP_001116956.1

Gene Ontology
Bibliography

Related articles in PubMed

[EXPRESS] Identification of interleukin-1 beta as a key mediator in the upregulation of Cav3.2-USP5 interactions in the pain pathway.
Stemkowski PL, Garcia-Caballero A, Gadotti VM, M'Dahoma S, Chen L, Souza IA, Zamponi GW
Molecular pain131744806917724698(2017 Jan-Dec)

TRPV1 Nociceptor Activity Initiates USP5/T-type Channel-Mediated Plasticity.
Stemkowski P, Garc?a-Caballero A, Gadotti VM, M'Dahoma S, Huang S, Black SAG, Chen L, Souza IA, Zhang Z, Zamponi GW
Cell reports17(11)2901-2912(2016 12)

Small organic molecule disruptors of Cav3.2 - USP5 interactions reverse inflammatory and neuropathic pain.
Gadotti VM, Caballero AG, Berger ND, Gladding CM, Chen L, Pfeifer TA, Zamponi GW
Molecular pain1112(2015 Mar)

The deubiquitinating enzyme USP5 modulates neuropathic and inflammatory pain by enhancing Cav3.2 channel activity.
Garc?a-Caballero A, Gadotti VM, Stemkowski P, Weiss N, Souza IA, Hodgkinson V, Bladen C, Chen L, Hamid J, Pizzoccaro A, Deage M, Fran?ois A, Bourinet E, Zamponi GW
Neuron83(5)1144-58(2014 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Sensitization was relieved by pharmacological block of TRPV1 afferents, but not of myelinated neurons. In spinal cord slice recordings, we could optogenetically trigger an activity-dependent potentiation of presynaptic neurotransmission in the spinal dorsal horn that relied on Cav3.2 channel activity. This neuronal-activity-induced USP5 upregulation may underlie a protective, transient sensitization of the pain pathway.
Title: TRPV1 Nociceptor Activity Initiates USP5/T-type Channel-Mediated Plasticity.

Suramin and Cav3.2/USP5 Tat-disruptor peptides were also tested in models of diabetic neuropathy and visceral pain, and provided remarkable protection
Title: Small organic molecule disruptors of Cav3.2 - USP5 interactions reverse inflammatory and neuropathic pain.

Knockdown and overexpression studies demonstrated that Usp5 regulates p53 (and p73) levels and alters cell growth and cell cycle distribution associated with p21 induction.
Title: Usp5 links suppression of p53 and FAS levels in melanoma to the BRAF pathway.

Local administration of NO may prevent neointimal hyperplasia by inhibiting isopeptidase T levels and activity in the vasculature, thereby inhibiting the 26S proteasome in vascular smooth muscle cells.
Title: Nitric oxide may inhibit neointimal hyperplasia by decreasing isopeptidase T levels and activity in the vasculature.

The following Usp5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Usp5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu09807	NM_013700.2 Latest version!	Mus musculus ubiquitin specific peptidase 5 (isopeptidase T) (Usp5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$503.30 $719.00
OMu37131	NM_001326594.1 Latest version!	Mus musculus ubiquitin specific peptidase 5 (isopeptidase T) (Usp5), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu37132	XM_030255317.1 Latest version!	Mus musculus ubiquitin specific peptidase 5 (isopeptidase T) (Usp5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu37132	XM_006505916.4 Latest version!	Mus musculus ubiquitin specific peptidase 5 (isopeptidase T) (Usp5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu89180	XM_030255318.1 Latest version!	Mus musculus ubiquitin specific peptidase 5 (isopeptidase T) (Usp5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OMu37132	XM_006505916.3	Mus musculus ubiquitin specific peptidase 5 (isopeptidase T) (Usp5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Usp5 ( NM_013700.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038728.1 Mus musculus ubiquitin specific peptidase 5 (isopeptidase T) (Usp5), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu09807
Clone ID Related Accession (Same CDS sequence)	NM_013700.2
Accession Version	NM_013700.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Mus musculus(house mouse)
Product	ubiquitin carboxyl-terminal hydrolase 5 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB855925.1, BC066993.1 and AA032751.1. On May 21, 2016 this sequence version replaced NM_013700.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC066993.1, SRR1660817.40439.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164138, SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGAGC TGAGTGAAGA GGCGCTGCTG TCAGTGTTAC CGACGATCCG TGTCCCCAAG 
GCGGGAGACC GGGTCCATAA AGACGAGTGC GCTTTCTCTT TCGACACGCC GGAGTCTGAG 
GGTGGCCTCT ATATCTGCAT GAACACATTC CTGGGATTCG GGAAGCAGTA TGTGGAGAGA 
CACTTCAACA AGACAGGCCA GCGTGTCTAC CTGCACCTCC GGAGGACCCG GCGACCGAAA 
GAAGAGGACA CCAGTGCAGG CACTGGAGAC CCACCTCGGA AGAAGCCCAC CCGGCTGGCC 
ATTGGTGTTG AAGGAGGGTT TGACCTCACC GAGGACAAGT TTGAATTTGA CGAGGATGTG 
AAGATTGTCA TTTTGCCCGA TTACCTGGAG ATCGCTCGGG ATGGGTTGGG GGGGCTTCCT 
GACATTGTCA GAGATCGGGT GACCAGTGCT GTGGAAGCCC TACTTTCTGC TGACTCAGCC 
TCTCGAAAGC AGGAGGTCCA GGCTTGGGAT GGGGAAGTCC GTCAGGTGTC CAAGCATGCC 
TTCAACCTCA AGCAGTTGGA CAACCCTGCT CGGATCCCTC CCTGTGGCTG GAAGTGCTCC 
AAGTGCGACA TGCGCGAGAA CCTGTGGCTC AACCTGACAG ACGGCTCCAT CCTCTGTGGA 
CGGCGCTACT TCGATGGCAG TGGGGGCAAC AACCATGCTG TGGAGCACTA CAGGGAGACT 
GGCTACCCCT TAGCTGTTAA GCTGGGCACC ATCACCCCGG ATGGAGCTGA CGTGTATTCT 
TACGACGAGG ATGACATGGT TCTGGACCCC AGCCTGGCAG AGCACTTGTC TCACTTTGGC 
ATCGACATGC TGAAGATGCA GAAGACGGAC AAGACGATGA CTGAGCTGGA GATAGACATG 
AACCAGCGGA TTGGTGAATG GGAGTTGATC CAGGAGTCAG GCGTGCCACT CAAGCCTCTG 
TTTGGGCCTG GTTACACGGG CATCCGGAAC CTGGGTAACA GCTGCTATCT CAACTCCGTG 
GTCCAGGTGC TCTTCAGCAT CCCTGACTTT CAGAGGAAGT ATGTGGATAA ATTGGAGAAG 
ATCTTCCAGA ATGCCCCAAC GGACCCCACC CAAGACTTCA GCACCCAGGT GGCCAAGCTG 
GGGCATGGCC TTCTCTCTGG AGAATACTCT AAGCCAGCAC TGGAGTCAGG TGATGGGGAG 
CAGGTACCAG AACAAAAGGA AGTTCAAGAT GGCATTGCCC CTCGAATGTT CAAGGCCCTC 
ATTGGCAAGG GCCACCCTGA GTTCTCCACT AATCGGCAGC AGGATGCCCA GGAATTCTTC 
CTACATCTTA TCAACATGGT GGAGAGGAAT TGCCGGAGTT CTGAAAATCC AAATGAAGTG 
TTCCGCTTTT TGGTGGAAGA AAAGATCAAA TGCCTGGCCA CAGAGAAGGT TAAGTACACT 
CAGCGAGTTG ACTACATCAT GCAGTTGCCT GTACCCATGG ATGCAGCCCT TAACAAAGAG 
GAGCTTTTAG AGTATGAAGA GAAGAAGCGG CAAGCTGAAG AGGAGAAAGT GCCACTGCCA 
GAACTGGTTC GGGCCCAGGT GCCCTTCAGC TCCTGCCTGG AAGCCTACGG GGCCCCTGAG 
CAGGTGGACG ACTTCTGGAG TACAGCTCTA CAGGCGAAGT CGGTCGCTGT CAAGACCACG 
CGCTTTGCCT CCTTCCCCGA CTACCTGGTC ATCCAGATCA AGAAGTTCAC CTTTGGCTTA 
GACTGGGTGC CCAAGAAGCT GGATGTGTCC ATTGAGATGC CAGAGGAGCT CGATATCTCC 
CAGCTGAGGG GCACAGGGCT ACAGCCGGGA GAGGAGGAGC TGCCTGACAT TGCCCCACCC 
CTGGTCACTC CGGATGAGCC CAAAGGTAGC CTTGGTTTCT ATGGCAACGA AGACGAAGAC 
TCCTTCTGCT CCCCTCACTT CTCCTCTCCG ACATCACCCA TGCTGGACGA ATCCGTCATC 
ATACAGTTGG TGGAAATGGG CTTCCCTATG GATGCCTGCC GGAAAGCTGT GTACTATACG 
GGAAACAGCG GGGCCGAAGC AGCCATGAAC TGGGTCATGT CACACATGGA TGATCCAGAT 
TTTGCAAACC CCCTCATCCT GCCTGGCTCC AGTGGGCCTG GCTCCACAAG TGCAGCAGCT 
GACCCCCCGC CGGAGGACTG TGTGACCACC ATAGTCTCCA TGGGCTTCTC CAGGGACCAG 
GCCCTGAAAG CTCTGCGGGC CACGAACAAT AGCTTAGAGC GGGCTGTAGA CTGGATCTTC 
AGCCACATTG ATGACCTGGA CGCAGAAGCT GCTATGGACA TCTCAGAGGG ACGCTCAGCG 
GCTGAGTCCA TCTCCGAGTC TGTGCCAGTG GGACCTAAAG TCCGGGATGG TCCTGGAAAG 
TATCAGCTCT TTGCCTTCAT TAGTCACATG GGCACATCTA CTATGTGTGG TCACTATGTC 
TGCCATATCA AGAAGGAAGG CAGGTGGGTG ATCTACAATG ACCAGAAAGT GTGTGCCTCT 
GAAAAGCCAC CCAAGGACCT GGGCTACATC TACTTCTACC AGAGAGTGGT CAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038728.1
CDS	90..2666
Translation	MAELSEEALLSVLPTIRVPKAGDRVHKDECAFSFDTPESEGGLYICMNTFLGFGKQYVERHFNKTGQRVYLHLRRTRRPKEEDTSAGTGDPPRKKPTRLAIGVEGGFDLTEDKFEFDEDVKIVILPDYLEIARDGLGGLPDIVRDRVTSAVEALLSADSASRKQEVQAWDGEVRQVSKHAFNLKQLDNPARIPPCGWKCSKCDMRENLWLNLTDGSILCGRRYFDGSGGNNHAVEHYRETGYPLAVKLGTITPDGADVYSYDEDDMVLDPSLAEHLSHFGIDMLKMQKTDKTMTELEIDMNQRIGEWELIQESGVPLKPLFGPGYTGIRNLGNSCYLNSVVQVLFSIPDFQRKYVDKLEKIFQNAPTDPTQDFSTQVAKLGHGLLSGEYSKPALESGDGEQVPEQKEVQDGIAPRMFKALIGKGHPEFSTNRQQDAQEFFLHLINMVERNCRSSENPNEVFRFLVEEKIKCLATEKVKYTQRVDYIMQLPVPMDAALNKEELLEYEEKKRQAEEEKVPLPELVRAQVPFSSCLEAYGAPEQVDDFWSTALQAKSVAVKTTRFASFPDYLVIQIKKFTFGLDWVPKKLDVSIEMPEELDISQLRGTGLQPGEEELPDIAPPLVTPDEPKGSLGFYGNEDEDSFCSPHFSSPTSPMLDESVIIQLVEMGFPMDACRKAVYYTGNSGAEAAMNWVMSHMDDPDFANPLILPGSSGPGSTSAAADPPPEDCVTTIVSMGFSRDQALKALRATNNSLERAVDWIFSHIDDLDAEAAMDISEGRSAAESISESVPVGPKVRDGPGKYQLFAFISHMGTSTMCGHYVCHIKKEGRWVIYNDQKVCASEKPPKDLGYIYFYQRVVS

Target ORF information:

RefSeq Version	NM_013700.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 5 (isopeptidase T) (Usp5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013700.2

ORF Insert Sequence:

ATGGCGGAGC TGAGTGAAGA GGCGCTGCTG TCAGTGTTAC CGACGATCCG TGTCCCCAAG 
GCGGGAGACC GGGTCCATAA AGACGAGTGC GCTTTCTCTT TCGACACGCC GGAGTCTGAG 
GGTGGCCTCT ATATCTGCAT GAACACATTC CTGGGATTCG GGAAGCAGTA TGTGGAGAGA 
CACTTCAACA AGACAGGCCA GCGTGTCTAC CTGCACCTCC GGAGGACCCG GCGACCGAAA 
GAAGAGGACA CCAGTGCAGG CACTGGAGAC CCACCTCGGA AGAAGCCCAC CCGGCTGGCC 
ATTGGTGTTG AAGGAGGGTT TGACCTCACC GAGGACAAGT TTGAATTTGA CGAGGATGTG 
AAGATTGTCA TTTTGCCCGA TTACCTGGAG ATCGCTCGGG ATGGGTTGGG GGGGCTTCCT 
GACATTGTCA GAGATCGGGT GACCAGTGCT GTGGAAGCCC TACTTTCTGC TGACTCAGCC 
TCTCGAAAGC AGGAGGTCCA GGCTTGGGAT GGGGAAGTCC GTCAGGTGTC CAAGCATGCC 
TTCAACCTCA AGCAGTTGGA CAACCCTGCT CGGATCCCTC CCTGTGGCTG GAAGTGCTCC 
AAGTGCGACA TGCGCGAGAA CCTGTGGCTC AACCTGACAG ACGGCTCCAT CCTCTGTGGA 
CGGCGCTACT TCGATGGCAG TGGGGGCAAC AACCATGCTG TGGAGCACTA CAGGGAGACT 
GGCTACCCCT TAGCTGTTAA GCTGGGCACC ATCACCCCGG ATGGAGCTGA CGTGTATTCT 
TACGACGAGG ATGACATGGT TCTGGACCCC AGCCTGGCAG AGCACTTGTC TCACTTTGGC 
ATCGACATGC TGAAGATGCA GAAGACGGAC AAGACGATGA CTGAGCTGGA GATAGACATG 
AACCAGCGGA TTGGTGAATG GGAGTTGATC CAGGAGTCAG GCGTGCCACT CAAGCCTCTG 
TTTGGGCCTG GTTACACGGG CATCCGGAAC CTGGGTAACA GCTGCTATCT CAACTCCGTG 
GTCCAGGTGC TCTTCAGCAT CCCTGACTTT CAGAGGAAGT ATGTGGATAA ATTGGAGAAG 
ATCTTCCAGA ATGCCCCAAC GGACCCCACC CAAGACTTCA GCACCCAGGT GGCCAAGCTG 
GGGCATGGCC TTCTCTCTGG AGAATACTCT AAGCCAGCAC TGGAGTCAGG TGATGGGGAG 
CAGGTACCAG AACAAAAGGA AGTTCAAGAT GGCATTGCCC CTCGAATGTT CAAGGCCCTC 
ATTGGCAAGG GCCACCCTGA GTTCTCCACT AATCGGCAGC AGGATGCCCA GGAATTCTTC 
CTACATCTTA TCAACATGGT GGAGAGGAAT TGCCGGAGTT CTGAAAATCC AAATGAAGTG 
TTCCGCTTTT TGGTGGAAGA AAAGATCAAA TGCCTGGCCA CAGAGAAGGT TAAGTACACT 
CAGCGAGTTG ACTACATCAT GCAGTTGCCT GTACCCATGG ATGCAGCCCT TAACAAAGAG 
GAGCTTTTAG AGTATGAAGA GAAGAAGCGG CAAGCTGAAG AGGAGAAAGT GCCACTGCCA 
GAACTGGTTC GGGCCCAGGT GCCCTTCAGC TCCTGCCTGG AAGCCTACGG GGCCCCTGAG 
CAGGTGGACG ACTTCTGGAG TACAGCTCTA CAGGCGAAGT CGGTCGCTGT CAAGACCACG 
CGCTTTGCCT CCTTCCCCGA CTACCTGGTC ATCCAGATCA AGAAGTTCAC CTTTGGCTTA 
GACTGGGTGC CCAAGAAGCT GGATGTGTCC ATTGAGATGC CAGAGGAGCT CGATATCTCC 
CAGCTGAGGG GCACAGGGCT ACAGCCGGGA GAGGAGGAGC TGCCTGACAT TGCCCCACCC 
CTGGTCACTC CGGATGAGCC CAAAGGTAGC CTTGGTTTCT ATGGCAACGA AGACGAAGAC 
TCCTTCTGCT CCCCTCACTT CTCCTCTCCG ACATCACCCA TGCTGGACGA ATCCGTCATC 
ATACAGTTGG TGGAAATGGG CTTCCCTATG GATGCCTGCC GGAAAGCTGT GTACTATACG 
GGAAACAGCG GGGCCGAAGC AGCCATGAAC TGGGTCATGT CACACATGGA TGATCCAGAT 
TTTGCAAACC CCCTCATCCT GCCTGGCTCC AGTGGGCCTG GCTCCACAAG TGCAGCAGCT 
GACCCCCCGC CGGAGGACTG TGTGACCACC ATAGTCTCCA TGGGCTTCTC CAGGGACCAG 
GCCCTGAAAG CTCTGCGGGC CACGAACAAT AGCTTAGAGC GGGCTGTAGA CTGGATCTTC 
AGCCACATTG ATGACCTGGA CGCAGAAGCT GCTATGGACA TCTCAGAGGG ACGCTCAGCG 
GCTGAGTCCA TCTCCGAGTC TGTGCCAGTG GGACCTAAAG TCCGGGATGG TCCTGGAAAG 
TATCAGCTCT TTGCCTTCAT TAGTCACATG GGCACATCTA CTATGTGTGG TCACTATGTC 
TGCCATATCA AGAAGGAAGG CAGGTGGGTG ATCTACAATG ACCAGAAAGT GTGTGCCTCT 
GAAAAGCCAC CCAAGGACCT GGGCTACATC TACTTCTACC AGAGAGTGGT CAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp5 ( NM_001326594.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001313523.1 Mus musculus ubiquitin specific peptidase 5 (isopeptidase T) (Usp5), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu37131
Clone ID Related Accession (Same CDS sequence)	NM_001326594.1
Accession Version	NM_001326594.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2508bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Mus musculus(house mouse)
Product	ubiquitin carboxyl-terminal hydrolase 5 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB855925.1, BC066993.1, CO426945.1 and AA032751.1. On May 21, 2016 this sequence version replaced XM_006505915.2. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded isoform (2), is shorter, but has the same N- and C-termini as isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK154008.1, SRR7345562.1403490.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164138, SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGAGC TGAGTGAAGA GGCGCTGCTG TCAGTGTTAC CGACGATCCG TGTCCCCAAG 
GCGGGAGACC GGGTCCATAA AGACGAGTGC GCTTTCTCTT TCGACACGCC GGAGTCTGAG 
GGTGGCCTCT ATATCTGCAT GAACACATTC CTGGGATTCG GGAAGCAGTA TGTGGAGAGA 
CACTTCAACA AGACAGGCCA GCGTGTCTAC CTGCACCTCC GGAGGACCCG GCGACCGAAA 
GAAGAGGACA CCAGTGCAGG CACTGGAGAC CCACCTCGGA AGAAGCCCAC CCGGCTGGCC 
ATTGGTGTTG AAGGAGGGTT TGACCTCACC GAGGACAAGT TTGAATTTGA CGAGGATGTG 
AAGATTGTCA TTTTGCCCGA TTACCTGGAG ATCGCTCGGG ATGGGTTGGG GGGGCTTCCT 
GACATTGTCA GAGATCGGGT GACCAGTGCT GTGGAAGCCC TACTTTCTGC TGACTCAGCC 
TCTCGAAAGC AGGAGGTCCA GGCTTGGGAT GGGGAAGTCC GTCAGGTGTC CAAGCATGCC 
TTCAACCTCA AGCAGTTGGA CAACCCTGCT CGGATCCCTC CCTGTGGCTG GAAGTGCTCC 
AAGTGCGACA TGCGCGAGAA CCTGTGGCTC AACCTGACAG ACGGCTCCAT CCTCTGTGGA 
CGGCGCTACT TCGATGGCAG TGGGGGCAAC AACCATGCTG TGGAGCACTA CAGGGAGACT 
GGCTACCCCT TAGCTGTTAA GCTGGGCACC ATCACCCCGG ATGGAGCTGA CGTGTATTCT 
TACGACGAGG ATGACATGGT TCTGGACCCC AGCCTGGCAG AGCACTTGTC TCACTTTGGC 
ATCGACATGC TGAAGATGCA GAAGACGGAC AAGACGATGA CTGAGCTGGA GATAGACATG 
AACCAGCGGA TTGGTGAATG GGAGTTGATC CAGGAGTCAG GCGTGCCACT CAAGCCTCTG 
TTTGGGCCTG GTTACACGGG CATCCGGAAC CTGGGTAACA GCTGCTATCT CAACTCCGTG 
GTCCAGGTGC TCTTCAGCAT CCCTGACTTT CAGAGGAAGT ATGTGGATAA ATTGGAGAAG 
ATCTTCCAGA ATGCCCCAAC GGACCCCACC CAAGACTTCA GCACCCAGGT GGCCAAGCTG 
GGGCATGGCC TTCTCTCTGG AGAATACTCT AAGCCAGCAC TGGAGTCAGG TGATGGGGAG 
CAGGTACCAG AACAAAAGGA AGTTCAAGAT GGCATTGCCC CTCGAATGTT CAAGGCCCTC 
ATTGGCAAGG GCCACCCTGA GTTCTCCACT AATCGGCAGC AGGATGCCCA GGAATTCTTC 
CTACATCTTA TCAACATGGT GGAGAGGAAT TGCCGGAGTT CTGAAAATCC AAATGAAGTG 
TTCCGCTTTT TGGTGGAAGA AAAGATCAAA TGCCTGGCCA CAGAGAAGGT TAAGTACACT 
CAGCGAGTTG ACTACATCAT GCAGTTGCCT GTACCCATGG ATGCAGCCCT TAACAAAGAG 
GAGCTTTTAG AGTATGAAGA GAAGAAGCGG CAAGCTGAAG AGGAGAAAGT GCCACTGCCA 
GAACTGGTTC GGGCCCAGGT GCCCTTCAGC TCCTGCCTGG AAGCCTACGG GGCCCCTGAG 
CAGGTGGACG ACTTCTGGAG TACAGCTCTA CAGGCGAAGT CGGTCGCTGT CAAGACCACG 
CGCTTTGCCT CCTTCCCCGA CTACCTGGTC ATCCAGATCA AGAAGTTCAC CTTTGGCTTA 
GACTGGGTGC CCAAGAAGCT GGATGTGTCC ATTGAGATGC CAGAGGAGCT CGATATCTCC 
CAGCTGAGGG GCACAGGGCT ACAGCCGGGA GAGGAGGAGC TGCCTGACAT TGCCCCACCC 
CTGGTCACTC CGGATGAGCC CAAAGCACCC ATGCTGGACG AATCCGTCAT CATACAGTTG 
GTGGAAATGG GCTTCCCTAT GGATGCCTGC CGGAAAGCTG TGTACTATAC GGGAAACAGC 
GGGGCCGAAG CAGCCATGAA CTGGGTCATG TCACACATGG ATGATCCAGA TTTTGCAAAC 
CCCCTCATCC TGCCTGGCTC CAGTGGGCCT GGCTCCACAA GTGCAGCAGC TGACCCCCCG 
CCGGAGGACT GTGTGACCAC CATAGTCTCC ATGGGCTTCT CCAGGGACCA GGCCCTGAAA 
GCTCTGCGGG CCACGAACAA TAGCTTAGAG CGGGCTGTAG ACTGGATCTT CAGCCACATT 
GATGACCTGG ACGCAGAAGC TGCTATGGAC ATCTCAGAGG GACGCTCAGC GGCTGAGTCC 
ATCTCCGAGT CTGTGCCAGT GGGACCTAAA GTCCGGGATG GTCCTGGAAA GTATCAGCTC 
TTTGCCTTCA TTAGTCACAT GGGCACATCT ACTATGTGTG GTCACTATGT CTGCCATATC 
AAGAAGGAAG GCAGGTGGGT GATCTACAAT GACCAGAAAG TGTGTGCCTC TGAAAAGCCA 
CCCAAGGACC TGGGCTACAT CTACTTCTAC CAGAGAGTGG TCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001313523.1
CDS	90..2597
Translation	MAELSEEALLSVLPTIRVPKAGDRVHKDECAFSFDTPESEGGLYICMNTFLGFGKQYVERHFNKTGQRVYLHLRRTRRPKEEDTSAGTGDPPRKKPTRLAIGVEGGFDLTEDKFEFDEDVKIVILPDYLEIARDGLGGLPDIVRDRVTSAVEALLSADSASRKQEVQAWDGEVRQVSKHAFNLKQLDNPARIPPCGWKCSKCDMRENLWLNLTDGSILCGRRYFDGSGGNNHAVEHYRETGYPLAVKLGTITPDGADVYSYDEDDMVLDPSLAEHLSHFGIDMLKMQKTDKTMTELEIDMNQRIGEWELIQESGVPLKPLFGPGYTGIRNLGNSCYLNSVVQVLFSIPDFQRKYVDKLEKIFQNAPTDPTQDFSTQVAKLGHGLLSGEYSKPALESGDGEQVPEQKEVQDGIAPRMFKALIGKGHPEFSTNRQQDAQEFFLHLINMVERNCRSSENPNEVFRFLVEEKIKCLATEKVKYTQRVDYIMQLPVPMDAALNKEELLEYEEKKRQAEEEKVPLPELVRAQVPFSSCLEAYGAPEQVDDFWSTALQAKSVAVKTTRFASFPDYLVIQIKKFTFGLDWVPKKLDVSIEMPEELDISQLRGTGLQPGEEELPDIAPPLVTPDEPKAPMLDESVIIQLVEMGFPMDACRKAVYYTGNSGAEAAMNWVMSHMDDPDFANPLILPGSSGPGSTSAAADPPPEDCVTTIVSMGFSRDQALKALRATNNSLERAVDWIFSHIDDLDAEAAMDISEGRSAAESISESVPVGPKVRDGPGKYQLFAFISHMGTSTMCGHYVCHIKKEGRWVIYNDQKVCASEKPPKDLGYIYFYQRVVS

Target ORF information:

RefSeq Version	NM_001326594.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 5 (isopeptidase T) (Usp5), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001326594.1

ORF Insert Sequence:

ATGGCGGAGC TGAGTGAAGA GGCGCTGCTG TCAGTGTTAC CGACGATCCG TGTCCCCAAG 
GCGGGAGACC GGGTCCATAA AGACGAGTGC GCTTTCTCTT TCGACACGCC GGAGTCTGAG 
GGTGGCCTCT ATATCTGCAT GAACACATTC CTGGGATTCG GGAAGCAGTA TGTGGAGAGA 
CACTTCAACA AGACAGGCCA GCGTGTCTAC CTGCACCTCC GGAGGACCCG GCGACCGAAA 
GAAGAGGACA CCAGTGCAGG CACTGGAGAC CCACCTCGGA AGAAGCCCAC CCGGCTGGCC 
ATTGGTGTTG AAGGAGGGTT TGACCTCACC GAGGACAAGT TTGAATTTGA CGAGGATGTG 
AAGATTGTCA TTTTGCCCGA TTACCTGGAG ATCGCTCGGG ATGGGTTGGG GGGGCTTCCT 
GACATTGTCA GAGATCGGGT GACCAGTGCT GTGGAAGCCC TACTTTCTGC TGACTCAGCC 
TCTCGAAAGC AGGAGGTCCA GGCTTGGGAT GGGGAAGTCC GTCAGGTGTC CAAGCATGCC 
TTCAACCTCA AGCAGTTGGA CAACCCTGCT CGGATCCCTC CCTGTGGCTG GAAGTGCTCC 
AAGTGCGACA TGCGCGAGAA CCTGTGGCTC AACCTGACAG ACGGCTCCAT CCTCTGTGGA 
CGGCGCTACT TCGATGGCAG TGGGGGCAAC AACCATGCTG TGGAGCACTA CAGGGAGACT 
GGCTACCCCT TAGCTGTTAA GCTGGGCACC ATCACCCCGG ATGGAGCTGA CGTGTATTCT 
TACGACGAGG ATGACATGGT TCTGGACCCC AGCCTGGCAG AGCACTTGTC TCACTTTGGC 
ATCGACATGC TGAAGATGCA GAAGACGGAC AAGACGATGA CTGAGCTGGA GATAGACATG 
AACCAGCGGA TTGGTGAATG GGAGTTGATC CAGGAGTCAG GCGTGCCACT CAAGCCTCTG 
TTTGGGCCTG GTTACACGGG CATCCGGAAC CTGGGTAACA GCTGCTATCT CAACTCCGTG 
GTCCAGGTGC TCTTCAGCAT CCCTGACTTT CAGAGGAAGT ATGTGGATAA ATTGGAGAAG 
ATCTTCCAGA ATGCCCCAAC GGACCCCACC CAAGACTTCA GCACCCAGGT GGCCAAGCTG 
GGGCATGGCC TTCTCTCTGG AGAATACTCT AAGCCAGCAC TGGAGTCAGG TGATGGGGAG 
CAGGTACCAG AACAAAAGGA AGTTCAAGAT GGCATTGCCC CTCGAATGTT CAAGGCCCTC 
ATTGGCAAGG GCCACCCTGA GTTCTCCACT AATCGGCAGC AGGATGCCCA GGAATTCTTC 
CTACATCTTA TCAACATGGT GGAGAGGAAT TGCCGGAGTT CTGAAAATCC AAATGAAGTG 
TTCCGCTTTT TGGTGGAAGA AAAGATCAAA TGCCTGGCCA CAGAGAAGGT TAAGTACACT 
CAGCGAGTTG ACTACATCAT GCAGTTGCCT GTACCCATGG ATGCAGCCCT TAACAAAGAG 
GAGCTTTTAG AGTATGAAGA GAAGAAGCGG CAAGCTGAAG AGGAGAAAGT GCCACTGCCA 
GAACTGGTTC GGGCCCAGGT GCCCTTCAGC TCCTGCCTGG AAGCCTACGG GGCCCCTGAG 
CAGGTGGACG ACTTCTGGAG TACAGCTCTA CAGGCGAAGT CGGTCGCTGT CAAGACCACG 
CGCTTTGCCT CCTTCCCCGA CTACCTGGTC ATCCAGATCA AGAAGTTCAC CTTTGGCTTA 
GACTGGGTGC CCAAGAAGCT GGATGTGTCC ATTGAGATGC CAGAGGAGCT CGATATCTCC 
CAGCTGAGGG GCACAGGGCT ACAGCCGGGA GAGGAGGAGC TGCCTGACAT TGCCCCACCC 
CTGGTCACTC CGGATGAGCC CAAAGCACCC ATGCTGGACG AATCCGTCAT CATACAGTTG 
GTGGAAATGG GCTTCCCTAT GGATGCCTGC CGGAAAGCTG TGTACTATAC GGGAAACAGC 
GGGGCCGAAG CAGCCATGAA CTGGGTCATG TCACACATGG ATGATCCAGA TTTTGCAAAC 
CCCCTCATCC TGCCTGGCTC CAGTGGGCCT GGCTCCACAA GTGCAGCAGC TGACCCCCCG 
CCGGAGGACT GTGTGACCAC CATAGTCTCC ATGGGCTTCT CCAGGGACCA GGCCCTGAAA 
GCTCTGCGGG CCACGAACAA TAGCTTAGAG CGGGCTGTAG ACTGGATCTT CAGCCACATT 
GATGACCTGG ACGCAGAAGC TGCTATGGAC ATCTCAGAGG GACGCTCAGC GGCTGAGTCC 
ATCTCCGAGT CTGTGCCAGT GGGACCTAAA GTCCGGGATG GTCCTGGAAA GTATCAGCTC 
TTTGCCTTCA TTAGTCACAT GGGCACATCT ACTATGTGTG GTCACTATGT CTGCCATATC 
AAGAAGGAAG GCAGGTGGGT GATCTACAAT GACCAGAAAG TGTGTGCCTC TGAAAAGCCA 
CCCAAGGACC TGGGCTACAT CTACTTCTAC CAGAGAGTGG TCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp5 ( XM_006505916.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006505979.1 Mus musculus ubiquitin specific peptidase 5 (isopeptidase T) (Usp5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu37132
Clone ID Related Accession (Same CDS sequence)	XM_006505916.3 , XM_006505916.4 , XM_030255317.1
Accession Version	XM_006505916.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2439bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ubiquitin carboxyl-terminal hydrolase 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006505916.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACACAT TCCTGGGATT CGGGAAGCAG TATGTGGAGA GACACTTCAA CAAGACAGGC 
CAGCGTGTCT ACCTGCACCT CCGGAGGACC CGGCGACCGA AAGAAGAGGA CACCAGTGCA 
GGCACTGGAG ACCCACCTCG GAAGAAGCCC ACCCGGCTGG CCATTGGTGT TGAAGGAGGG 
TTTGACCTCA CCGAGGACAA GTTTGAATTT GACGAGGATG TGAAGATTGT CATTTTGCCC 
GATTACCTGG AGATCGCTCG GGATGGGTTG GGGGGGCTTC CTGACATTGT CAGAGATCGG 
GTGACCAGTG CTGTGGAAGC CCTACTTTCT GCTGACTCAG CCTCTCGAAA GCAGGAGGTC 
CAGGCTTGGG ATGGGGAAGT CCGTCAGGTG TCCAAGCATG CCTTCAACCT CAAGCAGTTG 
GACAACCCTG CTCGGATCCC TCCCTGTGGC TGGAAGTGCT CCAAGTGCGA CATGCGCGAG 
AACCTGTGGC TCAACCTGAC AGACGGCTCC ATCCTCTGTG GACGGCGCTA CTTCGATGGC 
AGTGGGGGCA ACAACCATGC TGTGGAGCAC TACAGGGAGA CTGGCTACCC CTTAGCTGTT 
AAGCTGGGCA CCATCACCCC GGATGGAGCT GACGTGTATT CTTACGACGA GGATGACATG 
GTTCTGGACC CCAGCCTGGC AGAGCACTTG TCTCACTTTG GCATCGACAT GCTGAAGATG 
CAGAAGACGG ACAAGACGAT GACTGAGCTG GAGATAGACA TGAACCAGCG GATTGGTGAA 
TGGGAGTTGA TCCAGGAGTC AGGCGTGCCA CTCAAGCCTC TGTTTGGGCC TGGTTACACG 
GGCATCCGGA ACCTGGGTAA CAGCTGCTAT CTCAACTCCG TGGTCCAGGT GCTCTTCAGC 
ATCCCTGACT TTCAGAGGAA GTATGTGGAT AAATTGGAGA AGATCTTCCA GAATGCCCCA 
ACGGACCCCA CCCAAGACTT CAGCACCCAG GTGGCCAAGC TGGGGCATGG CCTTCTCTCT 
GGAGAATACT CTAAGCCAGC ACTGGAGTCA GGTGATGGGG AGCAGGTACC AGAACAAAAG 
GAAGTTCAAG ATGGCATTGC CCCTCGAATG TTCAAGGCCC TCATTGGCAA GGGCCACCCT 
GAGTTCTCCA CTAATCGGCA GCAGGATGCC CAGGAATTCT TCCTACATCT TATCAACATG 
GTGGAGAGGA ATTGCCGGAG TTCTGAAAAT CCAAATGAAG TGTTCCGCTT TTTGGTGGAA 
GAAAAGATCA AATGCCTGGC CACAGAGAAG GTTAAGTACA CTCAGCGAGT TGACTACATC 
ATGCAGTTGC CTGTACCCAT GGATGCAGCC CTTAACAAAG AGGAGCTTTT AGAGTATGAA 
GAGAAGAAGC GGCAAGCTGA AGAGGAGAAA GTGCCACTGC CAGAACTGGT TCGGGCCCAG 
GTGCCCTTCA GCTCCTGCCT GGAAGCCTAC GGGGCCCCTG AGCAGGTGGA CGACTTCTGG 
AGTACAGCTC TACAGGCGAA GTCGGTCGCT GTCAAGACCA CGCGCTTTGC CTCCTTCCCC 
GACTACCTGG TCATCCAGAT CAAGAAGTTC ACCTTTGGCT TAGACTGGGT GCCCAAGAAG 
CTGGATGTGT CCATTGAGAT GCCAGAGGAG CTCGATATCT CCCAGCTGAG GGGCACAGGG 
CTACAGCCGG GAGAGGAGGA GCTGCCTGAC ATTGCCCCAC CCCTGGTCAC TCCGGATGAG 
CCCAAAGGTA GCCTTGGTTT CTATGGCAAC GAAGACGAAG ACTCCTTCTG CTCCCCTCAC 
TTCTCCTCTC CGACATCACC CATGCTGGAC GAATCCGTCA TCATACAGTT GGTGGAAATG 
GGCTTCCCTA TGGATGCCTG CCGGAAAGCT GTGTACTATA CGGGAAACAG CGGGGCCGAA 
GCAGCCATGA ACTGGGTCAT GTCACACATG GATGATCCAG ATTTTGCAAA CCCCCTCATC 
CTGCCTGGCT CCAGTGGGCC TGGCTCCACA AGTGCAGCAG CTGACCCCCC GCCGGAGGAC 
TGTGTGACCA CCATAGTCTC CATGGGCTTC TCCAGGGACC AGGCCCTGAA AGCTCTGCGG 
GCCACGAACA ATAGCTTAGA GCGGGCTGTA GACTGGATCT TCAGCCACAT TGATGACCTG 
GACGCAGAAG CTGCTATGGA CATCTCAGAG GGACGCTCAG CGGCTGAGTC CATCTCCGAG 
TCTGTGCCAG TGGGACCTAA AGTCCGGGAT GGTCCTGGAA AGTATCAGCT CTTTGCCTTC 
ATTAGTCACA TGGGCACATC TACTATGTGT GGTCACTATG TCTGCCATAT CAAGAAGGAA 
GGCAGGTGGG TGATCTACAA TGACCAGAAA GTGTGTGCCT CTGAAAAGCC ACCCAAGGAC 
CTGGGCTACA TCTACTTCTA CCAGAGAGTG GTCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006505979.1
CDS	222..2660
Translation	MNTFLGFGKQYVERHFNKTGQRVYLHLRRTRRPKEEDTSAGTGDPPRKKPTRLAIGVEGGFDLTEDKFEFDEDVKIVILPDYLEIARDGLGGLPDIVRDRVTSAVEALLSADSASRKQEVQAWDGEVRQVSKHAFNLKQLDNPARIPPCGWKCSKCDMRENLWLNLTDGSILCGRRYFDGSGGNNHAVEHYRETGYPLAVKLGTITPDGADVYSYDEDDMVLDPSLAEHLSHFGIDMLKMQKTDKTMTELEIDMNQRIGEWELIQESGVPLKPLFGPGYTGIRNLGNSCYLNSVVQVLFSIPDFQRKYVDKLEKIFQNAPTDPTQDFSTQVAKLGHGLLSGEYSKPALESGDGEQVPEQKEVQDGIAPRMFKALIGKGHPEFSTNRQQDAQEFFLHLINMVERNCRSSENPNEVFRFLVEEKIKCLATEKVKYTQRVDYIMQLPVPMDAALNKEELLEYEEKKRQAEEEKVPLPELVRAQVPFSSCLEAYGAPEQVDDFWSTALQAKSVAVKTTRFASFPDYLVIQIKKFTFGLDWVPKKLDVSIEMPEELDISQLRGTGLQPGEEELPDIAPPLVTPDEPKGSLGFYGNEDEDSFCSPHFSSPTSPMLDESVIIQLVEMGFPMDACRKAVYYTGNSGAEAAMNWVMSHMDDPDFANPLILPGSSGPGSTSAAADPPPEDCVTTIVSMGFSRDQALKALRATNNSLERAVDWIFSHIDDLDAEAAMDISEGRSAAESISESVPVGPKVRDGPGKYQLFAFISHMGTSTMCGHYVCHIKKEGRWVIYNDQKVCASEKPPKDLGYIYFYQRVVS

Target ORF information:

RefSeq Version	XM_006505916.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 5 (isopeptidase T) (Usp5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006505916.3

ORF Insert Sequence:

ATGAACACAT TCCTGGGATT CGGGAAGCAG TATGTGGAGA GACACTTCAA CAAGACAGGC 
CAGCGTGTCT ACCTGCACCT CCGGAGGACC CGGCGACCGA AAGAAGAGGA CACCAGTGCA 
GGCACTGGAG ACCCACCTCG GAAGAAGCCC ACCCGGCTGG CCATTGGTGT TGAAGGAGGG 
TTTGACCTCA CCGAGGACAA GTTTGAATTT GACGAGGATG TGAAGATTGT CATTTTGCCC 
GATTACCTGG AGATCGCTCG GGATGGGTTG GGGGGGCTTC CTGACATTGT CAGAGATCGG 
GTGACCAGTG CTGTGGAAGC CCTACTTTCT GCTGACTCAG CCTCTCGAAA GCAGGAGGTC 
CAGGCTTGGG ATGGGGAAGT CCGTCAGGTG TCCAAGCATG CCTTCAACCT CAAGCAGTTG 
GACAACCCTG CTCGGATCCC TCCCTGTGGC TGGAAGTGCT CCAAGTGCGA CATGCGCGAG 
AACCTGTGGC TCAACCTGAC AGACGGCTCC ATCCTCTGTG GACGGCGCTA CTTCGATGGC 
AGTGGGGGCA ACAACCATGC TGTGGAGCAC TACAGGGAGA CTGGCTACCC CTTAGCTGTT 
AAGCTGGGCA CCATCACCCC GGATGGAGCT GACGTGTATT CTTACGACGA GGATGACATG 
GTTCTGGACC CCAGCCTGGC AGAGCACTTG TCTCACTTTG GCATCGACAT GCTGAAGATG 
CAGAAGACGG ACAAGACGAT GACTGAGCTG GAGATAGACA TGAACCAGCG GATTGGTGAA 
TGGGAGTTGA TCCAGGAGTC AGGCGTGCCA CTCAAGCCTC TGTTTGGGCC TGGTTACACG 
GGCATCCGGA ACCTGGGTAA CAGCTGCTAT CTCAACTCCG TGGTCCAGGT GCTCTTCAGC 
ATCCCTGACT TTCAGAGGAA GTATGTGGAT AAATTGGAGA AGATCTTCCA GAATGCCCCA 
ACGGACCCCA CCCAAGACTT CAGCACCCAG GTGGCCAAGC TGGGGCATGG CCTTCTCTCT 
GGAGAATACT CTAAGCCAGC ACTGGAGTCA GGTGATGGGG AGCAGGTACC AGAACAAAAG 
GAAGTTCAAG ATGGCATTGC CCCTCGAATG TTCAAGGCCC TCATTGGCAA GGGCCACCCT 
GAGTTCTCCA CTAATCGGCA GCAGGATGCC CAGGAATTCT TCCTACATCT TATCAACATG 
GTGGAGAGGA ATTGCCGGAG TTCTGAAAAT CCAAATGAAG TGTTCCGCTT TTTGGTGGAA 
GAAAAGATCA AATGCCTGGC CACAGAGAAG GTTAAGTACA CTCAGCGAGT TGACTACATC 
ATGCAGTTGC CTGTACCCAT GGATGCAGCC CTTAACAAAG AGGAGCTTTT AGAGTATGAA 
GAGAAGAAGC GGCAAGCTGA AGAGGAGAAA GTGCCACTGC CAGAACTGGT TCGGGCCCAG 
GTGCCCTTCA GCTCCTGCCT GGAAGCCTAC GGGGCCCCTG AGCAGGTGGA CGACTTCTGG 
AGTACAGCTC TACAGGCGAA GTCGGTCGCT GTCAAGACCA CGCGCTTTGC CTCCTTCCCC 
GACTACCTGG TCATCCAGAT CAAGAAGTTC ACCTTTGGCT TAGACTGGGT GCCCAAGAAG 
CTGGATGTGT CCATTGAGAT GCCAGAGGAG CTCGATATCT CCCAGCTGAG GGGCACAGGG 
CTACAGCCGG GAGAGGAGGA GCTGCCTGAC ATTGCCCCAC CCCTGGTCAC TCCGGATGAG 
CCCAAAGGTA GCCTTGGTTT CTATGGCAAC GAAGACGAAG ACTCCTTCTG CTCCCCTCAC 
TTCTCCTCTC CGACATCACC CATGCTGGAC GAATCCGTCA TCATACAGTT GGTGGAAATG 
GGCTTCCCTA TGGATGCCTG CCGGAAAGCT GTGTACTATA CGGGAAACAG CGGGGCCGAA 
GCAGCCATGA ACTGGGTCAT GTCACACATG GATGATCCAG ATTTTGCAAA CCCCCTCATC 
CTGCCTGGCT CCAGTGGGCC TGGCTCCACA AGTGCAGCAG CTGACCCCCC GCCGGAGGAC 
TGTGTGACCA CCATAGTCTC CATGGGCTTC TCCAGGGACC AGGCCCTGAA AGCTCTGCGG 
GCCACGAACA ATAGCTTAGA GCGGGCTGTA GACTGGATCT TCAGCCACAT TGATGACCTG 
GACGCAGAAG CTGCTATGGA CATCTCAGAG GGACGCTCAG CGGCTGAGTC CATCTCCGAG 
TCTGTGCCAG TGGGACCTAA AGTCCGGGAT GGTCCTGGAA AGTATCAGCT CTTTGCCTTC 
ATTAGTCACA TGGGCACATC TACTATGTGT GGTCACTATG TCTGCCATAT CAAGAAGGAA 
GGCAGGTGGG TGATCTACAA TGACCAGAAA GTGTGTGCCT CTGAAAAGCC ACCCAAGGAC 
CTGGGCTACA TCTACTTCTA CCAGAGAGTG GTCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp5 ( XM_006505916.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006505979.1 Mus musculus ubiquitin specific peptidase 5 (isopeptidase T) (Usp5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu37132
Clone ID Related Accession (Same CDS sequence)	XM_006505916.3 , XM_006505916.4 , XM_030255317.1
Accession Version	XM_006505916.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2439bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ubiquitin carboxyl-terminal hydrolase 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006505916.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACACAT TCCTGGGATT CGGGAAGCAG TATGTGGAGA GACACTTCAA CAAGACAGGC 
CAGCGTGTCT ACCTGCACCT CCGGAGGACC CGGCGACCGA AAGAAGAGGA CACCAGTGCA 
GGCACTGGAG ACCCACCTCG GAAGAAGCCC ACCCGGCTGG CCATTGGTGT TGAAGGAGGG 
TTTGACCTCA CCGAGGACAA GTTTGAATTT GACGAGGATG TGAAGATTGT CATTTTGCCC 
GATTACCTGG AGATCGCTCG GGATGGGTTG GGGGGGCTTC CTGACATTGT CAGAGATCGG 
GTGACCAGTG CTGTGGAAGC CCTACTTTCT GCTGACTCAG CCTCTCGAAA GCAGGAGGTC 
CAGGCTTGGG ATGGGGAAGT CCGTCAGGTG TCCAAGCATG CCTTCAACCT CAAGCAGTTG 
GACAACCCTG CTCGGATCCC TCCCTGTGGC TGGAAGTGCT CCAAGTGCGA CATGCGCGAG 
AACCTGTGGC TCAACCTGAC AGACGGCTCC ATCCTCTGTG GACGGCGCTA CTTCGATGGC 
AGTGGGGGCA ACAACCATGC TGTGGAGCAC TACAGGGAGA CTGGCTACCC CTTAGCTGTT 
AAGCTGGGCA CCATCACCCC GGATGGAGCT GACGTGTATT CTTACGACGA GGATGACATG 
GTTCTGGACC CCAGCCTGGC AGAGCACTTG TCTCACTTTG GCATCGACAT GCTGAAGATG 
CAGAAGACGG ACAAGACGAT GACTGAGCTG GAGATAGACA TGAACCAGCG GATTGGTGAA 
TGGGAGTTGA TCCAGGAGTC AGGCGTGCCA CTCAAGCCTC TGTTTGGGCC TGGTTACACG 
GGCATCCGGA ACCTGGGTAA CAGCTGCTAT CTCAACTCCG TGGTCCAGGT GCTCTTCAGC 
ATCCCTGACT TTCAGAGGAA GTATGTGGAT AAATTGGAGA AGATCTTCCA GAATGCCCCA 
ACGGACCCCA CCCAAGACTT CAGCACCCAG GTGGCCAAGC TGGGGCATGG CCTTCTCTCT 
GGAGAATACT CTAAGCCAGC ACTGGAGTCA GGTGATGGGG AGCAGGTACC AGAACAAAAG 
GAAGTTCAAG ATGGCATTGC CCCTCGAATG TTCAAGGCCC TCATTGGCAA GGGCCACCCT 
GAGTTCTCCA CTAATCGGCA GCAGGATGCC CAGGAATTCT TCCTACATCT TATCAACATG 
GTGGAGAGGA ATTGCCGGAG TTCTGAAAAT CCAAATGAAG TGTTCCGCTT TTTGGTGGAA 
GAAAAGATCA AATGCCTGGC CACAGAGAAG GTTAAGTACA CTCAGCGAGT TGACTACATC 
ATGCAGTTGC CTGTACCCAT GGATGCAGCC CTTAACAAAG AGGAGCTTTT AGAGTATGAA 
GAGAAGAAGC GGCAAGCTGA AGAGGAGAAA GTGCCACTGC CAGAACTGGT TCGGGCCCAG 
GTGCCCTTCA GCTCCTGCCT GGAAGCCTAC GGGGCCCCTG AGCAGGTGGA CGACTTCTGG 
AGTACAGCTC TACAGGCGAA GTCGGTCGCT GTCAAGACCA CGCGCTTTGC CTCCTTCCCC 
GACTACCTGG TCATCCAGAT CAAGAAGTTC ACCTTTGGCT TAGACTGGGT GCCCAAGAAG 
CTGGATGTGT CCATTGAGAT GCCAGAGGAG CTCGATATCT CCCAGCTGAG GGGCACAGGG 
CTACAGCCGG GAGAGGAGGA GCTGCCTGAC ATTGCCCCAC CCCTGGTCAC TCCGGATGAG 
CCCAAAGGTA GCCTTGGTTT CTATGGCAAC GAAGACGAAG ACTCCTTCTG CTCCCCTCAC 
TTCTCCTCTC CGACATCACC CATGCTGGAC GAATCCGTCA TCATACAGTT GGTGGAAATG 
GGCTTCCCTA TGGATGCCTG CCGGAAAGCT GTGTACTATA CGGGAAACAG CGGGGCCGAA 
GCAGCCATGA ACTGGGTCAT GTCACACATG GATGATCCAG ATTTTGCAAA CCCCCTCATC 
CTGCCTGGCT CCAGTGGGCC TGGCTCCACA AGTGCAGCAG CTGACCCCCC GCCGGAGGAC 
TGTGTGACCA CCATAGTCTC CATGGGCTTC TCCAGGGACC AGGCCCTGAA AGCTCTGCGG 
GCCACGAACA ATAGCTTAGA GCGGGCTGTA GACTGGATCT TCAGCCACAT TGATGACCTG 
GACGCAGAAG CTGCTATGGA CATCTCAGAG GGACGCTCAG CGGCTGAGTC CATCTCCGAG 
TCTGTGCCAG TGGGACCTAA AGTCCGGGAT GGTCCTGGAA AGTATCAGCT CTTTGCCTTC 
ATTAGTCACA TGGGCACATC TACTATGTGT GGTCACTATG TCTGCCATAT CAAGAAGGAA 
GGCAGGTGGG TGATCTACAA TGACCAGAAA GTGTGTGCCT CTGAAAAGCC ACCCAAGGAC 
CTGGGCTACA TCTACTTCTA CCAGAGAGTG GTCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006505979.1
CDS	238..2676
Translation	MNTFLGFGKQYVERHFNKTGQRVYLHLRRTRRPKEEDTSAGTGDPPRKKPTRLAIGVEGGFDLTEDKFEFDEDVKIVILPDYLEIARDGLGGLPDIVRDRVTSAVEALLSADSASRKQEVQAWDGEVRQVSKHAFNLKQLDNPARIPPCGWKCSKCDMRENLWLNLTDGSILCGRRYFDGSGGNNHAVEHYRETGYPLAVKLGTITPDGADVYSYDEDDMVLDPSLAEHLSHFGIDMLKMQKTDKTMTELEIDMNQRIGEWELIQESGVPLKPLFGPGYTGIRNLGNSCYLNSVVQVLFSIPDFQRKYVDKLEKIFQNAPTDPTQDFSTQVAKLGHGLLSGEYSKPALESGDGEQVPEQKEVQDGIAPRMFKALIGKGHPEFSTNRQQDAQEFFLHLINMVERNCRSSENPNEVFRFLVEEKIKCLATEKVKYTQRVDYIMQLPVPMDAALNKEELLEYEEKKRQAEEEKVPLPELVRAQVPFSSCLEAYGAPEQVDDFWSTALQAKSVAVKTTRFASFPDYLVIQIKKFTFGLDWVPKKLDVSIEMPEELDISQLRGTGLQPGEEELPDIAPPLVTPDEPKGSLGFYGNEDEDSFCSPHFSSPTSPMLDESVIIQLVEMGFPMDACRKAVYYTGNSGAEAAMNWVMSHMDDPDFANPLILPGSSGPGSTSAAADPPPEDCVTTIVSMGFSRDQALKALRATNNSLERAVDWIFSHIDDLDAEAAMDISEGRSAAESISESVPVGPKVRDGPGKYQLFAFISHMGTSTMCGHYVCHIKKEGRWVIYNDQKVCASEKPPKDLGYIYFYQRVVS

Target ORF information:

RefSeq Version	XM_006505916.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 5 (isopeptidase T) (Usp5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006505916.4

ORF Insert Sequence:

ATGAACACAT TCCTGGGATT CGGGAAGCAG TATGTGGAGA GACACTTCAA CAAGACAGGC 
CAGCGTGTCT ACCTGCACCT CCGGAGGACC CGGCGACCGA AAGAAGAGGA CACCAGTGCA 
GGCACTGGAG ACCCACCTCG GAAGAAGCCC ACCCGGCTGG CCATTGGTGT TGAAGGAGGG 
TTTGACCTCA CCGAGGACAA GTTTGAATTT GACGAGGATG TGAAGATTGT CATTTTGCCC 
GATTACCTGG AGATCGCTCG GGATGGGTTG GGGGGGCTTC CTGACATTGT CAGAGATCGG 
GTGACCAGTG CTGTGGAAGC CCTACTTTCT GCTGACTCAG CCTCTCGAAA GCAGGAGGTC 
CAGGCTTGGG ATGGGGAAGT CCGTCAGGTG TCCAAGCATG CCTTCAACCT CAAGCAGTTG 
GACAACCCTG CTCGGATCCC TCCCTGTGGC TGGAAGTGCT CCAAGTGCGA CATGCGCGAG 
AACCTGTGGC TCAACCTGAC AGACGGCTCC ATCCTCTGTG GACGGCGCTA CTTCGATGGC 
AGTGGGGGCA ACAACCATGC TGTGGAGCAC TACAGGGAGA CTGGCTACCC CTTAGCTGTT 
AAGCTGGGCA CCATCACCCC GGATGGAGCT GACGTGTATT CTTACGACGA GGATGACATG 
GTTCTGGACC CCAGCCTGGC AGAGCACTTG TCTCACTTTG GCATCGACAT GCTGAAGATG 
CAGAAGACGG ACAAGACGAT GACTGAGCTG GAGATAGACA TGAACCAGCG GATTGGTGAA 
TGGGAGTTGA TCCAGGAGTC AGGCGTGCCA CTCAAGCCTC TGTTTGGGCC TGGTTACACG 
GGCATCCGGA ACCTGGGTAA CAGCTGCTAT CTCAACTCCG TGGTCCAGGT GCTCTTCAGC 
ATCCCTGACT TTCAGAGGAA GTATGTGGAT AAATTGGAGA AGATCTTCCA GAATGCCCCA 
ACGGACCCCA CCCAAGACTT CAGCACCCAG GTGGCCAAGC TGGGGCATGG CCTTCTCTCT 
GGAGAATACT CTAAGCCAGC ACTGGAGTCA GGTGATGGGG AGCAGGTACC AGAACAAAAG 
GAAGTTCAAG ATGGCATTGC CCCTCGAATG TTCAAGGCCC TCATTGGCAA GGGCCACCCT 
GAGTTCTCCA CTAATCGGCA GCAGGATGCC CAGGAATTCT TCCTACATCT TATCAACATG 
GTGGAGAGGA ATTGCCGGAG TTCTGAAAAT CCAAATGAAG TGTTCCGCTT TTTGGTGGAA 
GAAAAGATCA AATGCCTGGC CACAGAGAAG GTTAAGTACA CTCAGCGAGT TGACTACATC 
ATGCAGTTGC CTGTACCCAT GGATGCAGCC CTTAACAAAG AGGAGCTTTT AGAGTATGAA 
GAGAAGAAGC GGCAAGCTGA AGAGGAGAAA GTGCCACTGC CAGAACTGGT TCGGGCCCAG 
GTGCCCTTCA GCTCCTGCCT GGAAGCCTAC GGGGCCCCTG AGCAGGTGGA CGACTTCTGG 
AGTACAGCTC TACAGGCGAA GTCGGTCGCT GTCAAGACCA CGCGCTTTGC CTCCTTCCCC 
GACTACCTGG TCATCCAGAT CAAGAAGTTC ACCTTTGGCT TAGACTGGGT GCCCAAGAAG 
CTGGATGTGT CCATTGAGAT GCCAGAGGAG CTCGATATCT CCCAGCTGAG GGGCACAGGG 
CTACAGCCGG GAGAGGAGGA GCTGCCTGAC ATTGCCCCAC CCCTGGTCAC TCCGGATGAG 
CCCAAAGGTA GCCTTGGTTT CTATGGCAAC GAAGACGAAG ACTCCTTCTG CTCCCCTCAC 
TTCTCCTCTC CGACATCACC CATGCTGGAC GAATCCGTCA TCATACAGTT GGTGGAAATG 
GGCTTCCCTA TGGATGCCTG CCGGAAAGCT GTGTACTATA CGGGAAACAG CGGGGCCGAA 
GCAGCCATGA ACTGGGTCAT GTCACACATG GATGATCCAG ATTTTGCAAA CCCCCTCATC 
CTGCCTGGCT CCAGTGGGCC TGGCTCCACA AGTGCAGCAG CTGACCCCCC GCCGGAGGAC 
TGTGTGACCA CCATAGTCTC CATGGGCTTC TCCAGGGACC AGGCCCTGAA AGCTCTGCGG 
GCCACGAACA ATAGCTTAGA GCGGGCTGTA GACTGGATCT TCAGCCACAT TGATGACCTG 
GACGCAGAAG CTGCTATGGA CATCTCAGAG GGACGCTCAG CGGCTGAGTC CATCTCCGAG 
TCTGTGCCAG TGGGACCTAA AGTCCGGGAT GGTCCTGGAA AGTATCAGCT CTTTGCCTTC 
ATTAGTCACA TGGGCACATC TACTATGTGT GGTCACTATG TCTGCCATAT CAAGAAGGAA 
GGCAGGTGGG TGATCTACAA TGACCAGAAA GTGTGTGCCT CTGAAAAGCC ACCCAAGGAC 
CTGGGCTACA TCTACTTCTA CCAGAGAGTG GTCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp5 ( XM_030255317.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030111177.1 Mus musculus ubiquitin specific peptidase 5 (isopeptidase T) (Usp5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu37132
Clone ID Related Accession (Same CDS sequence)	XM_006505916.3 , XM_006505916.4 , XM_030255317.1
Accession Version	XM_030255317.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2439bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ubiquitin carboxyl-terminal hydrolase 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACACAT TCCTGGGATT CGGGAAGCAG TATGTGGAGA GACACTTCAA CAAGACAGGC 
CAGCGTGTCT ACCTGCACCT CCGGAGGACC CGGCGACCGA AAGAAGAGGA CACCAGTGCA 
GGCACTGGAG ACCCACCTCG GAAGAAGCCC ACCCGGCTGG CCATTGGTGT TGAAGGAGGG 
TTTGACCTCA CCGAGGACAA GTTTGAATTT GACGAGGATG TGAAGATTGT CATTTTGCCC 
GATTACCTGG AGATCGCTCG GGATGGGTTG GGGGGGCTTC CTGACATTGT CAGAGATCGG 
GTGACCAGTG CTGTGGAAGC CCTACTTTCT GCTGACTCAG CCTCTCGAAA GCAGGAGGTC 
CAGGCTTGGG ATGGGGAAGT CCGTCAGGTG TCCAAGCATG CCTTCAACCT CAAGCAGTTG 
GACAACCCTG CTCGGATCCC TCCCTGTGGC TGGAAGTGCT CCAAGTGCGA CATGCGCGAG 
AACCTGTGGC TCAACCTGAC AGACGGCTCC ATCCTCTGTG GACGGCGCTA CTTCGATGGC 
AGTGGGGGCA ACAACCATGC TGTGGAGCAC TACAGGGAGA CTGGCTACCC CTTAGCTGTT 
AAGCTGGGCA CCATCACCCC GGATGGAGCT GACGTGTATT CTTACGACGA GGATGACATG 
GTTCTGGACC CCAGCCTGGC AGAGCACTTG TCTCACTTTG GCATCGACAT GCTGAAGATG 
CAGAAGACGG ACAAGACGAT GACTGAGCTG GAGATAGACA TGAACCAGCG GATTGGTGAA 
TGGGAGTTGA TCCAGGAGTC AGGCGTGCCA CTCAAGCCTC TGTTTGGGCC TGGTTACACG 
GGCATCCGGA ACCTGGGTAA CAGCTGCTAT CTCAACTCCG TGGTCCAGGT GCTCTTCAGC 
ATCCCTGACT TTCAGAGGAA GTATGTGGAT AAATTGGAGA AGATCTTCCA GAATGCCCCA 
ACGGACCCCA CCCAAGACTT CAGCACCCAG GTGGCCAAGC TGGGGCATGG CCTTCTCTCT 
GGAGAATACT CTAAGCCAGC ACTGGAGTCA GGTGATGGGG AGCAGGTACC AGAACAAAAG 
GAAGTTCAAG ATGGCATTGC CCCTCGAATG TTCAAGGCCC TCATTGGCAA GGGCCACCCT 
GAGTTCTCCA CTAATCGGCA GCAGGATGCC CAGGAATTCT TCCTACATCT TATCAACATG 
GTGGAGAGGA ATTGCCGGAG TTCTGAAAAT CCAAATGAAG TGTTCCGCTT TTTGGTGGAA 
GAAAAGATCA AATGCCTGGC CACAGAGAAG GTTAAGTACA CTCAGCGAGT TGACTACATC 
ATGCAGTTGC CTGTACCCAT GGATGCAGCC CTTAACAAAG AGGAGCTTTT AGAGTATGAA 
GAGAAGAAGC GGCAAGCTGA AGAGGAGAAA GTGCCACTGC CAGAACTGGT TCGGGCCCAG 
GTGCCCTTCA GCTCCTGCCT GGAAGCCTAC GGGGCCCCTG AGCAGGTGGA CGACTTCTGG 
AGTACAGCTC TACAGGCGAA GTCGGTCGCT GTCAAGACCA CGCGCTTTGC CTCCTTCCCC 
GACTACCTGG TCATCCAGAT CAAGAAGTTC ACCTTTGGCT TAGACTGGGT GCCCAAGAAG 
CTGGATGTGT CCATTGAGAT GCCAGAGGAG CTCGATATCT CCCAGCTGAG GGGCACAGGG 
CTACAGCCGG GAGAGGAGGA GCTGCCTGAC ATTGCCCCAC CCCTGGTCAC TCCGGATGAG 
CCCAAAGGTA GCCTTGGTTT CTATGGCAAC GAAGACGAAG ACTCCTTCTG CTCCCCTCAC 
TTCTCCTCTC CGACATCACC CATGCTGGAC GAATCCGTCA TCATACAGTT GGTGGAAATG 
GGCTTCCCTA TGGATGCCTG CCGGAAAGCT GTGTACTATA CGGGAAACAG CGGGGCCGAA 
GCAGCCATGA ACTGGGTCAT GTCACACATG GATGATCCAG ATTTTGCAAA CCCCCTCATC 
CTGCCTGGCT CCAGTGGGCC TGGCTCCACA AGTGCAGCAG CTGACCCCCC GCCGGAGGAC 
TGTGTGACCA CCATAGTCTC CATGGGCTTC TCCAGGGACC AGGCCCTGAA AGCTCTGCGG 
GCCACGAACA ATAGCTTAGA GCGGGCTGTA GACTGGATCT TCAGCCACAT TGATGACCTG 
GACGCAGAAG CTGCTATGGA CATCTCAGAG GGACGCTCAG CGGCTGAGTC CATCTCCGAG 
TCTGTGCCAG TGGGACCTAA AGTCCGGGAT GGTCCTGGAA AGTATCAGCT CTTTGCCTTC 
ATTAGTCACA TGGGCACATC TACTATGTGT GGTCACTATG TCTGCCATAT CAAGAAGGAA 
GGCAGGTGGG TGATCTACAA TGACCAGAAA GTGTGTGCCT CTGAAAAGCC ACCCAAGGAC 
CTGGGCTACA TCTACTTCTA CCAGAGAGTG GTCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030111177.1
CDS	273..2711
Translation	MNTFLGFGKQYVERHFNKTGQRVYLHLRRTRRPKEEDTSAGTGDPPRKKPTRLAIGVEGGFDLTEDKFEFDEDVKIVILPDYLEIARDGLGGLPDIVRDRVTSAVEALLSADSASRKQEVQAWDGEVRQVSKHAFNLKQLDNPARIPPCGWKCSKCDMRENLWLNLTDGSILCGRRYFDGSGGNNHAVEHYRETGYPLAVKLGTITPDGADVYSYDEDDMVLDPSLAEHLSHFGIDMLKMQKTDKTMTELEIDMNQRIGEWELIQESGVPLKPLFGPGYTGIRNLGNSCYLNSVVQVLFSIPDFQRKYVDKLEKIFQNAPTDPTQDFSTQVAKLGHGLLSGEYSKPALESGDGEQVPEQKEVQDGIAPRMFKALIGKGHPEFSTNRQQDAQEFFLHLINMVERNCRSSENPNEVFRFLVEEKIKCLATEKVKYTQRVDYIMQLPVPMDAALNKEELLEYEEKKRQAEEEKVPLPELVRAQVPFSSCLEAYGAPEQVDDFWSTALQAKSVAVKTTRFASFPDYLVIQIKKFTFGLDWVPKKLDVSIEMPEELDISQLRGTGLQPGEEELPDIAPPLVTPDEPKGSLGFYGNEDEDSFCSPHFSSPTSPMLDESVIIQLVEMGFPMDACRKAVYYTGNSGAEAAMNWVMSHMDDPDFANPLILPGSSGPGSTSAAADPPPEDCVTTIVSMGFSRDQALKALRATNNSLERAVDWIFSHIDDLDAEAAMDISEGRSAAESISESVPVGPKVRDGPGKYQLFAFISHMGTSTMCGHYVCHIKKEGRWVIYNDQKVCASEKPPKDLGYIYFYQRVVS

Target ORF information:

RefSeq Version	XM_030255317.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 5 (isopeptidase T) (Usp5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030255317.1

ORF Insert Sequence:

ATGAACACAT TCCTGGGATT CGGGAAGCAG TATGTGGAGA GACACTTCAA CAAGACAGGC 
CAGCGTGTCT ACCTGCACCT CCGGAGGACC CGGCGACCGA AAGAAGAGGA CACCAGTGCA 
GGCACTGGAG ACCCACCTCG GAAGAAGCCC ACCCGGCTGG CCATTGGTGT TGAAGGAGGG 
TTTGACCTCA CCGAGGACAA GTTTGAATTT GACGAGGATG TGAAGATTGT CATTTTGCCC 
GATTACCTGG AGATCGCTCG GGATGGGTTG GGGGGGCTTC CTGACATTGT CAGAGATCGG 
GTGACCAGTG CTGTGGAAGC CCTACTTTCT GCTGACTCAG CCTCTCGAAA GCAGGAGGTC 
CAGGCTTGGG ATGGGGAAGT CCGTCAGGTG TCCAAGCATG CCTTCAACCT CAAGCAGTTG 
GACAACCCTG CTCGGATCCC TCCCTGTGGC TGGAAGTGCT CCAAGTGCGA CATGCGCGAG 
AACCTGTGGC TCAACCTGAC AGACGGCTCC ATCCTCTGTG GACGGCGCTA CTTCGATGGC 
AGTGGGGGCA ACAACCATGC TGTGGAGCAC TACAGGGAGA CTGGCTACCC CTTAGCTGTT 
AAGCTGGGCA CCATCACCCC GGATGGAGCT GACGTGTATT CTTACGACGA GGATGACATG 
GTTCTGGACC CCAGCCTGGC AGAGCACTTG TCTCACTTTG GCATCGACAT GCTGAAGATG 
CAGAAGACGG ACAAGACGAT GACTGAGCTG GAGATAGACA TGAACCAGCG GATTGGTGAA 
TGGGAGTTGA TCCAGGAGTC AGGCGTGCCA CTCAAGCCTC TGTTTGGGCC TGGTTACACG 
GGCATCCGGA ACCTGGGTAA CAGCTGCTAT CTCAACTCCG TGGTCCAGGT GCTCTTCAGC 
ATCCCTGACT TTCAGAGGAA GTATGTGGAT AAATTGGAGA AGATCTTCCA GAATGCCCCA 
ACGGACCCCA CCCAAGACTT CAGCACCCAG GTGGCCAAGC TGGGGCATGG CCTTCTCTCT 
GGAGAATACT CTAAGCCAGC ACTGGAGTCA GGTGATGGGG AGCAGGTACC AGAACAAAAG 
GAAGTTCAAG ATGGCATTGC CCCTCGAATG TTCAAGGCCC TCATTGGCAA GGGCCACCCT 
GAGTTCTCCA CTAATCGGCA GCAGGATGCC CAGGAATTCT TCCTACATCT TATCAACATG 
GTGGAGAGGA ATTGCCGGAG TTCTGAAAAT CCAAATGAAG TGTTCCGCTT TTTGGTGGAA 
GAAAAGATCA AATGCCTGGC CACAGAGAAG GTTAAGTACA CTCAGCGAGT TGACTACATC 
ATGCAGTTGC CTGTACCCAT GGATGCAGCC CTTAACAAAG AGGAGCTTTT AGAGTATGAA 
GAGAAGAAGC GGCAAGCTGA AGAGGAGAAA GTGCCACTGC CAGAACTGGT TCGGGCCCAG 
GTGCCCTTCA GCTCCTGCCT GGAAGCCTAC GGGGCCCCTG AGCAGGTGGA CGACTTCTGG 
AGTACAGCTC TACAGGCGAA GTCGGTCGCT GTCAAGACCA CGCGCTTTGC CTCCTTCCCC 
GACTACCTGG TCATCCAGAT CAAGAAGTTC ACCTTTGGCT TAGACTGGGT GCCCAAGAAG 
CTGGATGTGT CCATTGAGAT GCCAGAGGAG CTCGATATCT CCCAGCTGAG GGGCACAGGG 
CTACAGCCGG GAGAGGAGGA GCTGCCTGAC ATTGCCCCAC CCCTGGTCAC TCCGGATGAG 
CCCAAAGGTA GCCTTGGTTT CTATGGCAAC GAAGACGAAG ACTCCTTCTG CTCCCCTCAC 
TTCTCCTCTC CGACATCACC CATGCTGGAC GAATCCGTCA TCATACAGTT GGTGGAAATG 
GGCTTCCCTA TGGATGCCTG CCGGAAAGCT GTGTACTATA CGGGAAACAG CGGGGCCGAA 
GCAGCCATGA ACTGGGTCAT GTCACACATG GATGATCCAG ATTTTGCAAA CCCCCTCATC 
CTGCCTGGCT CCAGTGGGCC TGGCTCCACA AGTGCAGCAG CTGACCCCCC GCCGGAGGAC 
TGTGTGACCA CCATAGTCTC CATGGGCTTC TCCAGGGACC AGGCCCTGAA AGCTCTGCGG 
GCCACGAACA ATAGCTTAGA GCGGGCTGTA GACTGGATCT TCAGCCACAT TGATGACCTG 
GACGCAGAAG CTGCTATGGA CATCTCAGAG GGACGCTCAG CGGCTGAGTC CATCTCCGAG 
TCTGTGCCAG TGGGACCTAA AGTCCGGGAT GGTCCTGGAA AGTATCAGCT CTTTGCCTTC 
ATTAGTCACA TGGGCACATC TACTATGTGT GGTCACTATG TCTGCCATAT CAAGAAGGAA 
GGCAGGTGGG TGATCTACAA TGACCAGAAA GTGTGTGCCT CTGAAAAGCC ACCCAAGGAC 
CTGGGCTACA TCTACTTCTA CCAGAGAGTG GTCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp5 ( XM_030255318.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030111178.1 Mus musculus ubiquitin specific peptidase 5 (isopeptidase T) (Usp5), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu89180
Clone ID Related Accession (Same CDS sequence)	XM_030255318.1
Accession Version	XM_030255318.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2370bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ubiquitin carboxyl-terminal hydrolase 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACACAT TCCTGGGATT CGGGAAGCAG TATGTGGAGA GACACTTCAA CAAGACAGGC 
CAGCGTGTCT ACCTGCACCT CCGGAGGACC CGGCGACCGA AAGAAGAGGA CACCAGTGCA 
GGCACTGGAG ACCCACCTCG GAAGAAGCCC ACCCGGCTGG CCATTGGTGT TGAAGGAGGG 
TTTGACCTCA CCGAGGACAA GTTTGAATTT GACGAGGATG TGAAGATTGT CATTTTGCCC 
GATTACCTGG AGATCGCTCG GGATGGGTTG GGGGGGCTTC CTGACATTGT CAGAGATCGG 
GTGACCAGTG CTGTGGAAGC CCTACTTTCT GCTGACTCAG CCTCTCGAAA GCAGGAGGTC 
CAGGCTTGGG ATGGGGAAGT CCGTCAGGTG TCCAAGCATG CCTTCAACCT CAAGCAGTTG 
GACAACCCTG CTCGGATCCC TCCCTGTGGC TGGAAGTGCT CCAAGTGCGA CATGCGCGAG 
AACCTGTGGC TCAACCTGAC AGACGGCTCC ATCCTCTGTG GACGGCGCTA CTTCGATGGC 
AGTGGGGGCA ACAACCATGC TGTGGAGCAC TACAGGGAGA CTGGCTACCC CTTAGCTGTT 
AAGCTGGGCA CCATCACCCC GGATGGAGCT GACGTGTATT CTTACGACGA GGATGACATG 
GTTCTGGACC CCAGCCTGGC AGAGCACTTG TCTCACTTTG GCATCGACAT GCTGAAGATG 
CAGAAGACGG ACAAGACGAT GACTGAGCTG GAGATAGACA TGAACCAGCG GATTGGTGAA 
TGGGAGTTGA TCCAGGAGTC AGGCGTGCCA CTCAAGCCTC TGTTTGGGCC TGGTTACACG 
GGCATCCGGA ACCTGGGTAA CAGCTGCTAT CTCAACTCCG TGGTCCAGGT GCTCTTCAGC 
ATCCCTGACT TTCAGAGGAA GTATGTGGAT AAATTGGAGA AGATCTTCCA GAATGCCCCA 
ACGGACCCCA CCCAAGACTT CAGCACCCAG GTGGCCAAGC TGGGGCATGG CCTTCTCTCT 
GGAGAATACT CTAAGCCAGC ACTGGAGTCA GGTGATGGGG AGCAGGTACC AGAACAAAAG 
GAAGTTCAAG ATGGCATTGC CCCTCGAATG TTCAAGGCCC TCATTGGCAA GGGCCACCCT 
GAGTTCTCCA CTAATCGGCA GCAGGATGCC CAGGAATTCT TCCTACATCT TATCAACATG 
GTGGAGAGGA ATTGCCGGAG TTCTGAAAAT CCAAATGAAG TGTTCCGCTT TTTGGTGGAA 
GAAAAGATCA AATGCCTGGC CACAGAGAAG GTTAAGTACA CTCAGCGAGT TGACTACATC 
ATGCAGTTGC CTGTACCCAT GGATGCAGCC CTTAACAAAG AGGAGCTTTT AGAGTATGAA 
GAGAAGAAGC GGCAAGCTGA AGAGGAGAAA GTGCCACTGC CAGAACTGGT TCGGGCCCAG 
GTGCCCTTCA GCTCCTGCCT GGAAGCCTAC GGGGCCCCTG AGCAGGTGGA CGACTTCTGG 
AGTACAGCTC TACAGGCGAA GTCGGTCGCT GTCAAGACCA CGCGCTTTGC CTCCTTCCCC 
GACTACCTGG TCATCCAGAT CAAGAAGTTC ACCTTTGGCT TAGACTGGGT GCCCAAGAAG 
CTGGATGTGT CCATTGAGAT GCCAGAGGAG CTCGATATCT CCCAGCTGAG GGGCACAGGG 
CTACAGCCGG GAGAGGAGGA GCTGCCTGAC ATTGCCCCAC CCCTGGTCAC TCCGGATGAG 
CCCAAAGCAC CCATGCTGGA CGAATCCGTC ATCATACAGT TGGTGGAAAT GGGCTTCCCT 
ATGGATGCCT GCCGGAAAGC TGTGTACTAT ACGGGAAACA GCGGGGCCGA AGCAGCCATG 
AACTGGGTCA TGTCACACAT GGATGATCCA GATTTTGCAA ACCCCCTCAT CCTGCCTGGC 
TCCAGTGGGC CTGGCTCCAC AAGTGCAGCA GCTGACCCCC CGCCGGAGGA CTGTGTGACC 
ACCATAGTCT CCATGGGCTT CTCCAGGGAC CAGGCCCTGA AAGCTCTGCG GGCCACGAAC 
AATAGCTTAG AGCGGGCTGT AGACTGGATC TTCAGCCACA TTGATGACCT GGACGCAGAA 
GCTGCTATGG ACATCTCAGA GGGACGCTCA GCGGCTGAGT CCATCTCCGA GTCTGTGCCA 
GTGGGACCTA AAGTCCGGGA TGGTCCTGGA AAGTATCAGC TCTTTGCCTT CATTAGTCAC 
ATGGGCACAT CTACTATGTG TGGTCACTAT GTCTGCCATA TCAAGAAGGA AGGCAGGTGG 
GTGATCTACA ATGACCAGAA AGTGTGTGCC TCTGAAAAGC CACCCAAGGA CCTGGGCTAC 
ATCTACTTCT ACCAGAGAGT GGTCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030111178.1
CDS	238..2607
Translation	MNTFLGFGKQYVERHFNKTGQRVYLHLRRTRRPKEEDTSAGTGDPPRKKPTRLAIGVEGGFDLTEDKFEFDEDVKIVILPDYLEIARDGLGGLPDIVRDRVTSAVEALLSADSASRKQEVQAWDGEVRQVSKHAFNLKQLDNPARIPPCGWKCSKCDMRENLWLNLTDGSILCGRRYFDGSGGNNHAVEHYRETGYPLAVKLGTITPDGADVYSYDEDDMVLDPSLAEHLSHFGIDMLKMQKTDKTMTELEIDMNQRIGEWELIQESGVPLKPLFGPGYTGIRNLGNSCYLNSVVQVLFSIPDFQRKYVDKLEKIFQNAPTDPTQDFSTQVAKLGHGLLSGEYSKPALESGDGEQVPEQKEVQDGIAPRMFKALIGKGHPEFSTNRQQDAQEFFLHLINMVERNCRSSENPNEVFRFLVEEKIKCLATEKVKYTQRVDYIMQLPVPMDAALNKEELLEYEEKKRQAEEEKVPLPELVRAQVPFSSCLEAYGAPEQVDDFWSTALQAKSVAVKTTRFASFPDYLVIQIKKFTFGLDWVPKKLDVSIEMPEELDISQLRGTGLQPGEEELPDIAPPLVTPDEPKAPMLDESVIIQLVEMGFPMDACRKAVYYTGNSGAEAAMNWVMSHMDDPDFANPLILPGSSGPGSTSAAADPPPEDCVTTIVSMGFSRDQALKALRATNNSLERAVDWIFSHIDDLDAEAAMDISEGRSAAESISESVPVGPKVRDGPGKYQLFAFISHMGTSTMCGHYVCHIKKEGRWVIYNDQKVCASEKPPKDLGYIYFYQRVVS

Target ORF information:

RefSeq Version	XM_030255318.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 5 (isopeptidase T) (Usp5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030255318.1

ORF Insert Sequence:

ATGAACACAT TCCTGGGATT CGGGAAGCAG TATGTGGAGA GACACTTCAA CAAGACAGGC 
CAGCGTGTCT ACCTGCACCT CCGGAGGACC CGGCGACCGA AAGAAGAGGA CACCAGTGCA 
GGCACTGGAG ACCCACCTCG GAAGAAGCCC ACCCGGCTGG CCATTGGTGT TGAAGGAGGG 
TTTGACCTCA CCGAGGACAA GTTTGAATTT GACGAGGATG TGAAGATTGT CATTTTGCCC 
GATTACCTGG AGATCGCTCG GGATGGGTTG GGGGGGCTTC CTGACATTGT CAGAGATCGG 
GTGACCAGTG CTGTGGAAGC CCTACTTTCT GCTGACTCAG CCTCTCGAAA GCAGGAGGTC 
CAGGCTTGGG ATGGGGAAGT CCGTCAGGTG TCCAAGCATG CCTTCAACCT CAAGCAGTTG 
GACAACCCTG CTCGGATCCC TCCCTGTGGC TGGAAGTGCT CCAAGTGCGA CATGCGCGAG 
AACCTGTGGC TCAACCTGAC AGACGGCTCC ATCCTCTGTG GACGGCGCTA CTTCGATGGC 
AGTGGGGGCA ACAACCATGC TGTGGAGCAC TACAGGGAGA CTGGCTACCC CTTAGCTGTT 
AAGCTGGGCA CCATCACCCC GGATGGAGCT GACGTGTATT CTTACGACGA GGATGACATG 
GTTCTGGACC CCAGCCTGGC AGAGCACTTG TCTCACTTTG GCATCGACAT GCTGAAGATG 
CAGAAGACGG ACAAGACGAT GACTGAGCTG GAGATAGACA TGAACCAGCG GATTGGTGAA 
TGGGAGTTGA TCCAGGAGTC AGGCGTGCCA CTCAAGCCTC TGTTTGGGCC TGGTTACACG 
GGCATCCGGA ACCTGGGTAA CAGCTGCTAT CTCAACTCCG TGGTCCAGGT GCTCTTCAGC 
ATCCCTGACT TTCAGAGGAA GTATGTGGAT AAATTGGAGA AGATCTTCCA GAATGCCCCA 
ACGGACCCCA CCCAAGACTT CAGCACCCAG GTGGCCAAGC TGGGGCATGG CCTTCTCTCT 
GGAGAATACT CTAAGCCAGC ACTGGAGTCA GGTGATGGGG AGCAGGTACC AGAACAAAAG 
GAAGTTCAAG ATGGCATTGC CCCTCGAATG TTCAAGGCCC TCATTGGCAA GGGCCACCCT 
GAGTTCTCCA CTAATCGGCA GCAGGATGCC CAGGAATTCT TCCTACATCT TATCAACATG 
GTGGAGAGGA ATTGCCGGAG TTCTGAAAAT CCAAATGAAG TGTTCCGCTT TTTGGTGGAA 
GAAAAGATCA AATGCCTGGC CACAGAGAAG GTTAAGTACA CTCAGCGAGT TGACTACATC 
ATGCAGTTGC CTGTACCCAT GGATGCAGCC CTTAACAAAG AGGAGCTTTT AGAGTATGAA 
GAGAAGAAGC GGCAAGCTGA AGAGGAGAAA GTGCCACTGC CAGAACTGGT TCGGGCCCAG 
GTGCCCTTCA GCTCCTGCCT GGAAGCCTAC GGGGCCCCTG AGCAGGTGGA CGACTTCTGG 
AGTACAGCTC TACAGGCGAA GTCGGTCGCT GTCAAGACCA CGCGCTTTGC CTCCTTCCCC 
GACTACCTGG TCATCCAGAT CAAGAAGTTC ACCTTTGGCT TAGACTGGGT GCCCAAGAAG 
CTGGATGTGT CCATTGAGAT GCCAGAGGAG CTCGATATCT CCCAGCTGAG GGGCACAGGG 
CTACAGCCGG GAGAGGAGGA GCTGCCTGAC ATTGCCCCAC CCCTGGTCAC TCCGGATGAG 
CCCAAAGCAC CCATGCTGGA CGAATCCGTC ATCATACAGT TGGTGGAAAT GGGCTTCCCT 
ATGGATGCCT GCCGGAAAGC TGTGTACTAT ACGGGAAACA GCGGGGCCGA AGCAGCCATG 
AACTGGGTCA TGTCACACAT GGATGATCCA GATTTTGCAA ACCCCCTCAT CCTGCCTGGC 
TCCAGTGGGC CTGGCTCCAC AAGTGCAGCA GCTGACCCCC CGCCGGAGGA CTGTGTGACC 
ACCATAGTCT CCATGGGCTT CTCCAGGGAC CAGGCCCTGA AAGCTCTGCG GGCCACGAAC 
AATAGCTTAG AGCGGGCTGT AGACTGGATC TTCAGCCACA TTGATGACCT GGACGCAGAA 
GCTGCTATGG ACATCTCAGA GGGACGCTCA GCGGCTGAGT CCATCTCCGA GTCTGTGCCA 
GTGGGACCTA AAGTCCGGGA TGGTCCTGGA AAGTATCAGC TCTTTGCCTT CATTAGTCAC 
ATGGGCACAT CTACTATGTG TGGTCACTAT GTCTGCCATA TCAAGAAGGA AGGCAGGTGG 
GTGATCTACA ATGACCAGAA AGTGTGTGCC TCTGAAAAGC CACCCAAGGA CCTGGGCTAC 
ATCTACTTCT ACCAGAGAGT GGTCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

[EXPRESS] Identification of interleukin-1 beta as a key mediator in the upregulation of Cav3.2-USP5 interactions in the pain pathway.
Molecular pain131744806917724698(2017 Jan-Dec)
Stemkowski PL,Garcia-Caballero A,Gadotti VM,M'Dahoma S,Chen L,Souza IA,Zamponi GW

TRPV1 Nociceptor Activity Initiates USP5/T-type Channel-Mediated Plasticity.
Cell reports17(11)2901-2912(2016 12)
Stemkowski P,Garc?a-Caballero A,Gadotti VM,M'Dahoma S,Huang S,Black SAG,Chen L,Souza IA,Zhang Z,Zamponi GW

Small organic molecule disruptors of Cav3.2 - USP5 interactions reverse inflammatory and neuropathic pain.
Molecular pain1112(2015 Mar)
Gadotti VM,Caballero AG,Berger ND,Gladding CM,Chen L,Pfeifer TA,Zamponi GW

The deubiquitinating enzyme USP5 modulates neuropathic and inflammatory pain by enhancing Cav3.2 channel activity.
Neuron83(5)1144-58(2014 Sep)
Garc?a-Caballero A,Gadotti VM,Stemkowski P,Weiss N,Souza IA,Hodgkinson V,Bladen C,Chen L,Hamid J,Pizzoccaro A,Deage M,Fran?ois A,Bourinet E,Zamponi GW

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Usp5 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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