Ubr1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ubr1
Entrez Gene ID	22222
Full Name	ubiquitin protein ligase E3 component n-recognin 1
Synonyms	AI504731
General protein information	Preferred Names ubiquitin protein ligase E3 component n-recognin 1 Names E3 ubiquitin-protein ligase UBR1 E3 alpha N-recognin-1 RING-type E3 ubiquitin transferase UBR1 ubiquitin-protein ligase E3-alpha-1 ubiquitin-protein ligase E3-alpha-I ubiquitin-protein ligase e3 componen n-recognin
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2 E5\|2 60.37 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006499165.3	XP_006499228.1	E3 ubiquitin-protein ligase UBR1 isoform X2
XM_011239440.2	XP_011237742.1	E3 ubiquitin-protein ligase UBR1 isoform X3
XM_011239440.3	XP_011237742.1	E3 ubiquitin-protein ligase UBR1 isoform X2
XM_006499164.3	XP_006499227.1	E3 ubiquitin-protein ligase UBR1 isoform X1
XM_006499164.2	XP_006499227.1	E3 ubiquitin-protein ligase UBR1 isoform X1
NM_009461.2	NP_033487.2	E3 ubiquitin-protein ligase UBR1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	UBR1	XP_510341.3
	UBR1	XP_001105721.2
Canis lupus familiaris (dog)	UBR1	XP_544645.2
Bos taurus (cattle)	UBR1	NP_001192826.1
Danio rerio (zebrafish)	ubr1	XP_002665264.3
Xenopus tropicalis (tropical clawed frog)	ubr1	XP_002941132.2
Rattus norvegicus (Norway rat)	Ubr1	NP_001171543.1
Homo sapiens (human)	UBR1	NP_777576.1
Mus musculus (house mouse)	Ubr1	NP_033487.2
Gallus gallus (chicken)	UBR1	XP_421165.3
Schizosaccharomyces pombe (fission yeast)	ubr1	NP_596158.2
Magnaporthe oryzae (rice blast fungus)	MGG_13171	XP_003710667.1
Neurospora crassa	NCU03234	XP_965334.2

Gene Ontology
Bibliography

Related articles in PubMed

The N-end rule pathway counteracts cell death by destroying proapoptotic protein fragments.
Piatkov KI, Brower CS, Varshavsky A
Proceedings of the National Academy of Sciences of the United States of America109(27)E1839-47(2012 Jul)

The substrate recognition domains of the N-end rule pathway.
Tasaki T, Zakrzewska A, Dudgeon DD, Jiang Y, Lazo JS, Kwon YT
The Journal of biological chemistry284(3)1884-95(2009 Jan)

Roles of STAT3/SOCS3 pathway in regulating the visual function and ubiquitin-proteasome-dependent degradation of rhodopsin during retinal inflammation.
Ozawa Y, Nakao K, Kurihara T, Shimazaki T, Shimmura S, Ishida S, Yoshimura A, Tsubota K, Okano H
The Journal of biological chemistry283(36)24561-70(2008 Sep)

Synthetic heterovalent inhibitors targeting recognition E3 components of the N-end rule pathway.
Lee MJ, Pal K, Tasaki T, Roy S, Jiang Y, An JY, Banerjee R, Kwon YT
Proceedings of the National Academy of Sciences of the United States of America105(1)100-5(2008 Jan)

Biochemical and genetic studies of UBR3, a ubiquitin ligase with a function in olfactory and other sensory systems.
Tasaki T, Sohr R, Xia Z, Hellweg R, H?rtnagl H, Varshavsky A, Kwon YT
The Journal of biological chemistry282(25)18510-20(2007 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A STAT3-dependent E3 ubiquitin ligase, Ubr1, was responsible for rhodopsin degradation and was up-regulated in the inflamed SOCS3-deficient retinas.
Title: Roles of STAT3/SOCS3 pathway in regulating the visual function and ubiquitin-proteasome-dependent degradation of rhodopsin during retinal inflammation.

TLX1/HOX11 overexpression and Ubr1 inactivation in promoting chromosome missegregation, permitting the accrual of additional chromosome losses and cytogenic abnormalities en route to malignancy
Title: Loss of Ubr1 promotes aneuploidy and accelerates B-cell lymphomagenesis in TLX1/HOX11-transgenic mice.

UBR1-/-UBR2-/- embryos die at midgestation, with defects in neurogenesis and cardiovascular development.
Title: Impaired neurogenesis and cardiovascular development in mice lacking the E3 ubiquitin ligases UBR1 and UBR2 of the N-end rule pathway.

involved in learning and memory; behavioral characterizations of UBR1 knockout mice
Title: Behavioral characterization of mice lacking the ubiquitin ligase UBR1 of the N-end rule pathway.

The following Ubr1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ubr1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu69007	XM_011239440.3 Latest version!	Mus musculus ubiquitin protein ligase E3 component n-recognin 1 (Ubr1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu30250	XM_006499165.3 Latest version!	Mus musculus ubiquitin protein ligase E3 component n-recognin 1 (Ubr1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu30249	XM_006499164.3 Latest version!	Mus musculus ubiquitin protein ligase E3 component n-recognin 1 (Ubr1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu04208	NM_009461.2 Latest version!	Mus musculus ubiquitin protein ligase E3 component n-recognin 1 (Ubr1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
View Old Accession Versions >>
OMu69007	XM_011239440.2	Mus musculus ubiquitin protein ligase E3 component n-recognin 1 (Ubr1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu30249	XM_006499164.2	Mus musculus ubiquitin protein ligase E3 component n-recognin 1 (Ubr1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ubr1 ( XM_011239440.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011237742.1 Mus musculus ubiquitin protein ligase E3 component n-recognin 1 (Ubr1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu69007
Clone ID Related Accession (Same CDS sequence)	XM_011239440.2 , XM_011239440.3
Accession Version	XM_011239440.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2970bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase UBR1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011239440.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGAGA TTACTCACTT ACTTTGCATT GAGCCCATGC CACATAGTGC CATCGCCAGA 
AACCTACCTG AGAACGAAAA TAATGAAACT GGCTTAGAGA ATGTCATAAA CAAAGTGGCC 
ACATTTAAGA AACCAGGTGT GTCGGGCCAT GGAGTTTATG AATTGAAAGA TGAATCACTG 
AAAGACTTCA ATATGTACTT TTACCATTAT TCTAAAACAC AGCATAGCAA GGCTGAACAT 
ATGCAGAAGA AAAGGAGAAA ACAAGAAAAT AAAGATGAAG CATTGCCGCC GCCACCTCCT 
CCGGAGTTCT GCCCTGCTTT CAGCAAAGTA GTCAACCTGC TCAGCTGTGA TGTTATGATG 
TACATCCTCA GGACCATCTT TGAGCGGGCA GTGGACATGG AGTCTAATCT GTGGACAGAA 
GGGATGCTGC AGATGGCGTT CCATATATTG GCACTGGGCT TGCTGGAAGA GAAGCAGCAG 
CTTCAGAAAG CTCCTGAAGA GGAAGTGGCT TTTGACTTTT ACCATAAGGC TTCAAGATTG 
GGAAGTTCAG CCATGAATGC TCAGAATATA CAAATGCTCT TGGAAAAACT CAAAGGAATC 
CCCCAATTAG AAAGCCAGAA GGACATGATA ACATGGATAC TCCAGATGTT TGACACAGTG 
AAGCGATTAA GAGAAAAATC TTGTTTAGTT GTGGCAACCA CTTCAGGACT GGAGTGCGTT 
AAGAGTGAGG AGATTACTCA TGATAAAGAA AAGGCAGAAA GGAAGAGAAA AGCTGAGGCC 
GCTAGGCTTC ATCGCCAGAA GATCATGGCC CAGATGTCTG CCTTACAGAA AAACTTCATT 
GAAACCCACA AACTCATGTA TGATAATACG TCAGAAGTAA CAGGGAAGGA AGACTCCATT 
ATGGAGGAAG AGAGCACCTC AGCAGTCAGT GAGGCCTCTA GAATTGCTCT GGGCCCTAAA 
CGGGGCCCGG CTGTTACTGA AAAGGAGGTG CTGACGTGCA TCCTCTGCCA AGAAGAACAA 
GAGGTGAAAC TAGAAAATAA CGCCATGGTA TTGTCAGCAT GTGTGCAGAA ATCCACCGCC 
CTAACCCAGC ACAGAGGGAA GCCTGTGGAC CACTTAGGGG AAACACTGGA CCCTCTTTTC 
ATGGATCCAG ACTTGGCACA TGGAACTTAT ACAGGAAGCT GTGGTCATGT AATGCATGCA 
GTGTGCTGGC AGAAGTATTT TGAAGCTGTG CAGCTGAGCT CGCAGCAGCG CATTCACGTA 
GACCTGTTTG ACCTGGAGAG CGGCGAGTAC CTATGCCCGC TCTGCAAGTC TCTCTGCAAT 
ACTGTCATCC CCATCATCCC TTTGCAGCCG CAGAAGATCA ACAGTGAGAA TGCGGAGGCT 
CTTGCTCAAC TTTTGACCTT GGCCCGGTGG ATACAGACTG TCCTTGCCAG AATATCGGGT 
TATAATATAA AGCATGCTAA AGGAGAAGCC CCAGCAGTTC CTGTCTTGTT TAATCAAGGA 
ATGGGGGATT CGACTTTTGA GTTTCATTCC ATCCTGAGTT TTGGAGTTCA GTCTTCGGTG 
AAATATTCAA ATAGTATCAA GGAAATGGTC ATTCTCTTCG CCACAACAAT TTACAGAATT 
GGCCTGAAAG TGCCTCCTGA TGAACTAGAC CCACGAGTGC CCATGATGAC CTGGAGCACG 
TGTGCGTTCA CCATCCAGGC AATCGAAAAC CTGTTGGGAG ATGAAGGAAA ACCTCTATTT 
GGAGCACTTC AAAATAGACA GCATAACGGT CTGAAGGCGC TAATGCAGTT TGCAGTTGCA 
CAGAGGACTA CCTGCCCTCA GGTCCTGATA CACAAACATC TGGCTCGGCT CCTGTCAGTT 
ATTCTTCCTA ACCTGCAATC AGAAAATACA CCAGGCCTTC TGTCCGTGGA TCTCTTCCAT 
GTTCTGGTCG GCGCAGTCTT AGCGTTCCCA TCCTTGTATT GGGATGACAC CGTGGATCTG 
CAGCCGTCAC CACTTAGTTC TTCATATAAC CACCTCTATC TCTTCCATCT GATCACCATG 
GCGCACATGC TTCAGATACT CCTTACAACA GATACAGATC TGTCTTCAGG GCCGCCGCTT 
GCTGAGGGTG AAGAGGATAG TGAGGAGGCT CGCTGTGCAT CTGCTTTCTT TGTGGAAGTG 
TCGCAGCACA CAGACGGCCT CGCTGGGTGC GGTGCTCCCG GCTGGTACCT GTGGCTCTCC 
CTGAGGAACG GCATCACCCC TTACCTCCGC TGTGCTGCAC TGCTTTTCCA CTATTTACTT 
GGAGTAGCTC CGCCTGAAGA ACTGTTTGCC AATTCTGCTG AAGGAGAATT CAGTGCACTC 
TGTAGCTATC TATCTTTACC CACAAATTTG TTCCTGCTTT TCCAGGAATA TTGGGATACC 
ATAAGGCCCT TACTACAGAG GTGGTGTGGA GATCCTGCCT TACTCAAGTC TTTGAAGCAG 
AAAAGTGCTG TGGTCAGGTA CCCTAGAAAA AGAAATAGTT TGATAGAGCT TCCTGAGGAC 
TACAGCTGTC TTCTAAATCA GGCTTCTCAC TTTAGGTGTC CACGGTCTGC AGATGATGAG 
CGAAAGCATC CTGTCCTCTG TCTTTTCTGT GGGGCCATCC TGTGTTCTCA GAACATCTGT 
TGCCAAGAAA TAGTGAATGG GGAAGAGGTT GGAGCGTGCG TTTTTCATGC GCTTCATTGT 
GGTGCTGGAG TCTGCATTTT CTTAAAAATC CGAGAATGCA GGGTGGTCCT GGTGGAAGGA 
AAAGCCAGAG GCTGTGCCTA CCCAGCCCCT TACTTGGATG AATATGGAGA AACAGACCCA 
GGGCTAAAGA GAGGAAACCC ACTTCATTTA TCTCGGGAGC GGTATCGGAA GCTGCATTTG 
GTCTGGCAAC AGCACTGCAT TATAGAAGAG ATTGCTCGGA GCCAGGAGAC TAATCAGATG 
CTATTTGGAT TCAACTGGCA GTTACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011237742.1
CDS	275..3244
Translation	MREITHLLCIEPMPHSAIARNLPENENNETGLENVINKVATFKKPGVSGHGVYELKDESLKDFNMYFYHYSKTQHSKAEHMQKKRRKQENKDEALPPPPPPEFCPAFSKVVNLLSCDVMMYILRTIFERAVDMESNLWTEGMLQMAFHILALGLLEEKQQLQKAPEEEVAFDFYHKASRLGSSAMNAQNIQMLLEKLKGIPQLESQKDMITWILQMFDTVKRLREKSCLVVATTSGLECVKSEEITHDKEKAERKRKAEAARLHRQKIMAQMSALQKNFIETHKLMYDNTSEVTGKEDSIMEEESTSAVSEASRIALGPKRGPAVTEKEVLTCILCQEEQEVKLENNAMVLSACVQKSTALTQHRGKPVDHLGETLDPLFMDPDLAHGTYTGSCGHVMHAVCWQKYFEAVQLSSQQRIHVDLFDLESGEYLCPLCKSLCNTVIPIIPLQPQKINSENAEALAQLLTLARWIQTVLARISGYNIKHAKGEAPAVPVLFNQGMGDSTFEFHSILSFGVQSSVKYSNSIKEMVILFATTIYRIGLKVPPDELDPRVPMMTWSTCAFTIQAIENLLGDEGKPLFGALQNRQHNGLKALMQFAVAQRTTCPQVLIHKHLARLLSVILPNLQSENTPGLLSVDLFHVLVGAVLAFPSLYWDDTVDLQPSPLSSSYNHLYLFHLITMAHMLQILLTTDTDLSSGPPLAEGEEDSEEARCASAFFVEVSQHTDGLAGCGAPGWYLWLSLRNGITPYLRCAALLFHYLLGVAPPEELFANSAEGEFSALCSYLSLPTNLFLLFQEYWDTIRPLLQRWCGDPALLKSLKQKSAVVRYPRKRNSLIELPEDYSCLLNQASHFRCPRSADDERKHPVLCLFCGAILCSQNICCQEIVNGEEVGACVFHALHCGAGVCIFLKIRECRVVLVEGKARGCAYPAPYLDEYGETDPGLKRGNPLHLSRERYRKLHLVWQQHCIIEEIARSQETNQMLFGFNWQLL

Target ORF information:

RefSeq Version	XM_011239440.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin protein ligase E3 component n-recognin 1 (Ubr1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011239440.2

ORF Insert Sequence:

ATGAGAGAGA TTACTCACTT ACTTTGCATT GAGCCCATGC CACATAGTGC CATCGCCAGA 
AACCTACCTG AGAACGAAAA TAATGAAACT GGCTTAGAGA ATGTCATAAA CAAAGTGGCC 
ACATTTAAGA AACCAGGTGT GTCGGGCCAT GGAGTTTATG AATTGAAAGA TGAATCACTG 
AAAGACTTCA ATATGTACTT TTACCATTAT TCTAAAACAC AGCATAGCAA GGCTGAACAT 
ATGCAGAAGA AAAGGAGAAA ACAAGAAAAT AAAGATGAAG CATTGCCGCC GCCACCTCCT 
CCGGAGTTCT GCCCTGCTTT CAGCAAAGTA GTCAACCTGC TCAGCTGTGA TGTTATGATG 
TACATCCTCA GGACCATCTT TGAGCGGGCA GTGGACATGG AGTCTAATCT GTGGACAGAA 
GGGATGCTGC AGATGGCGTT CCATATATTG GCACTGGGCT TGCTGGAAGA GAAGCAGCAG 
CTTCAGAAAG CTCCTGAAGA GGAAGTGGCT TTTGACTTTT ACCATAAGGC TTCAAGATTG 
GGAAGTTCAG CCATGAATGC TCAGAATATA CAAATGCTCT TGGAAAAACT CAAAGGAATC 
CCCCAATTAG AAAGCCAGAA GGACATGATA ACATGGATAC TCCAGATGTT TGACACAGTG 
AAGCGATTAA GAGAAAAATC TTGTTTAGTT GTGGCAACCA CTTCAGGACT GGAGTGCGTT 
AAGAGTGAGG AGATTACTCA TGATAAAGAA AAGGCAGAAA GGAAGAGAAA AGCTGAGGCC 
GCTAGGCTTC ATCGCCAGAA GATCATGGCC CAGATGTCTG CCTTACAGAA AAACTTCATT 
GAAACCCACA AACTCATGTA TGATAATACG TCAGAAGTAA CAGGGAAGGA AGACTCCATT 
ATGGAGGAAG AGAGCACCTC AGCAGTCAGT GAGGCCTCTA GAATTGCTCT GGGCCCTAAA 
CGGGGCCCGG CTGTTACTGA AAAGGAGGTG CTGACGTGCA TCCTCTGCCA AGAAGAACAA 
GAGGTGAAAC TAGAAAATAA CGCCATGGTA TTGTCAGCAT GTGTGCAGAA ATCCACCGCC 
CTAACCCAGC ACAGAGGGAA GCCTGTGGAC CACTTAGGGG AAACACTGGA CCCTCTTTTC 
ATGGATCCAG ACTTGGCACA TGGAACTTAT ACAGGAAGCT GTGGTCATGT AATGCATGCA 
GTGTGCTGGC AGAAGTATTT TGAAGCTGTG CAGCTGAGCT CGCAGCAGCG CATTCACGTA 
GACCTGTTTG ACCTGGAGAG CGGCGAGTAC CTATGCCCGC TCTGCAAGTC TCTCTGCAAT 
ACTGTCATCC CCATCATCCC TTTGCAGCCG CAGAAGATCA ACAGTGAGAA TGCGGAGGCT 
CTTGCTCAAC TTTTGACCTT GGCCCGGTGG ATACAGACTG TCCTTGCCAG AATATCGGGT 
TATAATATAA AGCATGCTAA AGGAGAAGCC CCAGCAGTTC CTGTCTTGTT TAATCAAGGA 
ATGGGGGATT CGACTTTTGA GTTTCATTCC ATCCTGAGTT TTGGAGTTCA GTCTTCGGTG 
AAATATTCAA ATAGTATCAA GGAAATGGTC ATTCTCTTCG CCACAACAAT TTACAGAATT 
GGCCTGAAAG TGCCTCCTGA TGAACTAGAC CCACGAGTGC CCATGATGAC CTGGAGCACG 
TGTGCGTTCA CCATCCAGGC AATCGAAAAC CTGTTGGGAG ATGAAGGAAA ACCTCTATTT 
GGAGCACTTC AAAATAGACA GCATAACGGT CTGAAGGCGC TAATGCAGTT TGCAGTTGCA 
CAGAGGACTA CCTGCCCTCA GGTCCTGATA CACAAACATC TGGCTCGGCT CCTGTCAGTT 
ATTCTTCCTA ACCTGCAATC AGAAAATACA CCAGGCCTTC TGTCCGTGGA TCTCTTCCAT 
GTTCTGGTCG GCGCAGTCTT AGCGTTCCCA TCCTTGTATT GGGATGACAC CGTGGATCTG 
CAGCCGTCAC CACTTAGTTC TTCATATAAC CACCTCTATC TCTTCCATCT GATCACCATG 
GCGCACATGC TTCAGATACT CCTTACAACA GATACAGATC TGTCTTCAGG GCCGCCGCTT 
GCTGAGGGTG AAGAGGATAG TGAGGAGGCT CGCTGTGCAT CTGCTTTCTT TGTGGAAGTG 
TCGCAGCACA CAGACGGCCT CGCTGGGTGC GGTGCTCCCG GCTGGTACCT GTGGCTCTCC 
CTGAGGAACG GCATCACCCC TTACCTCCGC TGTGCTGCAC TGCTTTTCCA CTATTTACTT 
GGAGTAGCTC CGCCTGAAGA ACTGTTTGCC AATTCTGCTG AAGGAGAATT CAGTGCACTC 
TGTAGCTATC TATCTTTACC CACAAATTTG TTCCTGCTTT TCCAGGAATA TTGGGATACC 
ATAAGGCCCT TACTACAGAG GTGGTGTGGA GATCCTGCCT TACTCAAGTC TTTGAAGCAG 
AAAAGTGCTG TGGTCAGGTA CCCTAGAAAA AGAAATAGTT TGATAGAGCT TCCTGAGGAC 
TACAGCTGTC TTCTAAATCA GGCTTCTCAC TTTAGGTGTC CACGGTCTGC AGATGATGAG 
CGAAAGCATC CTGTCCTCTG TCTTTTCTGT GGGGCCATCC TGTGTTCTCA GAACATCTGT 
TGCCAAGAAA TAGTGAATGG GGAAGAGGTT GGAGCGTGCG TTTTTCATGC GCTTCATTGT 
GGTGCTGGAG TCTGCATTTT CTTAAAAATC CGAGAATGCA GGGTGGTCCT GGTGGAAGGA 
AAAGCCAGAG GCTGTGCCTA CCCAGCCCCT TACTTGGATG AATATGGAGA AACAGACCCA 
GGGCTAAAGA GAGGAAACCC ACTTCATTTA TCTCGGGAGC GGTATCGGAA GCTGCATTTG 
GTCTGGCAAC AGCACTGCAT TATAGAAGAG ATTGCTCGGA GCCAGGAGAC TAATCAGATG 
CTATTTGGAT TCAACTGGCA GTTACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ubr1 ( XM_011239440.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011237742.1 Mus musculus ubiquitin protein ligase E3 component n-recognin 1 (Ubr1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu69007
Clone ID Related Accession (Same CDS sequence)	XM_011239440.2 , XM_011239440.3
Accession Version	XM_011239440.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2970bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase UBR1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011239440.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGAGA TTACTCACTT ACTTTGCATT GAGCCCATGC CACATAGTGC CATCGCCAGA 
AACCTACCTG AGAACGAAAA TAATGAAACT GGCTTAGAGA ATGTCATAAA CAAAGTGGCC 
ACATTTAAGA AACCAGGTGT GTCGGGCCAT GGAGTTTATG AATTGAAAGA TGAATCACTG 
AAAGACTTCA ATATGTACTT TTACCATTAT TCTAAAACAC AGCATAGCAA GGCTGAACAT 
ATGCAGAAGA AAAGGAGAAA ACAAGAAAAT AAAGATGAAG CATTGCCGCC GCCACCTCCT 
CCGGAGTTCT GCCCTGCTTT CAGCAAAGTA GTCAACCTGC TCAGCTGTGA TGTTATGATG 
TACATCCTCA GGACCATCTT TGAGCGGGCA GTGGACATGG AGTCTAATCT GTGGACAGAA 
GGGATGCTGC AGATGGCGTT CCATATATTG GCACTGGGCT TGCTGGAAGA GAAGCAGCAG 
CTTCAGAAAG CTCCTGAAGA GGAAGTGGCT TTTGACTTTT ACCATAAGGC TTCAAGATTG 
GGAAGTTCAG CCATGAATGC TCAGAATATA CAAATGCTCT TGGAAAAACT CAAAGGAATC 
CCCCAATTAG AAAGCCAGAA GGACATGATA ACATGGATAC TCCAGATGTT TGACACAGTG 
AAGCGATTAA GAGAAAAATC TTGTTTAGTT GTGGCAACCA CTTCAGGACT GGAGTGCGTT 
AAGAGTGAGG AGATTACTCA TGATAAAGAA AAGGCAGAAA GGAAGAGAAA AGCTGAGGCC 
GCTAGGCTTC ATCGCCAGAA GATCATGGCC CAGATGTCTG CCTTACAGAA AAACTTCATT 
GAAACCCACA AACTCATGTA TGATAATACG TCAGAAGTAA CAGGGAAGGA AGACTCCATT 
ATGGAGGAAG AGAGCACCTC AGCAGTCAGT GAGGCCTCTA GAATTGCTCT GGGCCCTAAA 
CGGGGCCCGG CTGTTACTGA AAAGGAGGTG CTGACGTGCA TCCTCTGCCA AGAAGAACAA 
GAGGTGAAAC TAGAAAATAA CGCCATGGTA TTGTCAGCAT GTGTGCAGAA ATCCACCGCC 
CTAACCCAGC ACAGAGGGAA GCCTGTGGAC CACTTAGGGG AAACACTGGA CCCTCTTTTC 
ATGGATCCAG ACTTGGCACA TGGAACTTAT ACAGGAAGCT GTGGTCATGT AATGCATGCA 
GTGTGCTGGC AGAAGTATTT TGAAGCTGTG CAGCTGAGCT CGCAGCAGCG CATTCACGTA 
GACCTGTTTG ACCTGGAGAG CGGCGAGTAC CTATGCCCGC TCTGCAAGTC TCTCTGCAAT 
ACTGTCATCC CCATCATCCC TTTGCAGCCG CAGAAGATCA ACAGTGAGAA TGCGGAGGCT 
CTTGCTCAAC TTTTGACCTT GGCCCGGTGG ATACAGACTG TCCTTGCCAG AATATCGGGT 
TATAATATAA AGCATGCTAA AGGAGAAGCC CCAGCAGTTC CTGTCTTGTT TAATCAAGGA 
ATGGGGGATT CGACTTTTGA GTTTCATTCC ATCCTGAGTT TTGGAGTTCA GTCTTCGGTG 
AAATATTCAA ATAGTATCAA GGAAATGGTC ATTCTCTTCG CCACAACAAT TTACAGAATT 
GGCCTGAAAG TGCCTCCTGA TGAACTAGAC CCACGAGTGC CCATGATGAC CTGGAGCACG 
TGTGCGTTCA CCATCCAGGC AATCGAAAAC CTGTTGGGAG ATGAAGGAAA ACCTCTATTT 
GGAGCACTTC AAAATAGACA GCATAACGGT CTGAAGGCGC TAATGCAGTT TGCAGTTGCA 
CAGAGGACTA CCTGCCCTCA GGTCCTGATA CACAAACATC TGGCTCGGCT CCTGTCAGTT 
ATTCTTCCTA ACCTGCAATC AGAAAATACA CCAGGCCTTC TGTCCGTGGA TCTCTTCCAT 
GTTCTGGTCG GCGCAGTCTT AGCGTTCCCA TCCTTGTATT GGGATGACAC CGTGGATCTG 
CAGCCGTCAC CACTTAGTTC TTCATATAAC CACCTCTATC TCTTCCATCT GATCACCATG 
GCGCACATGC TTCAGATACT CCTTACAACA GATACAGATC TGTCTTCAGG GCCGCCGCTT 
GCTGAGGGTG AAGAGGATAG TGAGGAGGCT CGCTGTGCAT CTGCTTTCTT TGTGGAAGTG 
TCGCAGCACA CAGACGGCCT CGCTGGGTGC GGTGCTCCCG GCTGGTACCT GTGGCTCTCC 
CTGAGGAACG GCATCACCCC TTACCTCCGC TGTGCTGCAC TGCTTTTCCA CTATTTACTT 
GGAGTAGCTC CGCCTGAAGA ACTGTTTGCC AATTCTGCTG AAGGAGAATT CAGTGCACTC 
TGTAGCTATC TATCTTTACC CACAAATTTG TTCCTGCTTT TCCAGGAATA TTGGGATACC 
ATAAGGCCCT TACTACAGAG GTGGTGTGGA GATCCTGCCT TACTCAAGTC TTTGAAGCAG 
AAAAGTGCTG TGGTCAGGTA CCCTAGAAAA AGAAATAGTT TGATAGAGCT TCCTGAGGAC 
TACAGCTGTC TTCTAAATCA GGCTTCTCAC TTTAGGTGTC CACGGTCTGC AGATGATGAG 
CGAAAGCATC CTGTCCTCTG TCTTTTCTGT GGGGCCATCC TGTGTTCTCA GAACATCTGT 
TGCCAAGAAA TAGTGAATGG GGAAGAGGTT GGAGCGTGCG TTTTTCATGC GCTTCATTGT 
GGTGCTGGAG TCTGCATTTT CTTAAAAATC CGAGAATGCA GGGTGGTCCT GGTGGAAGGA 
AAAGCCAGAG GCTGTGCCTA CCCAGCCCCT TACTTGGATG AATATGGAGA AACAGACCCA 
GGGCTAAAGA GAGGAAACCC ACTTCATTTA TCTCGGGAGC GGTATCGGAA GCTGCATTTG 
GTCTGGCAAC AGCACTGCAT TATAGAAGAG ATTGCTCGGA GCCAGGAGAC TAATCAGATG 
CTATTTGGAT TCAACTGGCA GTTACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011237742.1
CDS	275..3244
Translation	MREITHLLCIEPMPHSAIARNLPENENNETGLENVINKVATFKKPGVSGHGVYELKDESLKDFNMYFYHYSKTQHSKAEHMQKKRRKQENKDEALPPPPPPEFCPAFSKVVNLLSCDVMMYILRTIFERAVDMESNLWTEGMLQMAFHILALGLLEEKQQLQKAPEEEVAFDFYHKASRLGSSAMNAQNIQMLLEKLKGIPQLESQKDMITWILQMFDTVKRLREKSCLVVATTSGLECVKSEEITHDKEKAERKRKAEAARLHRQKIMAQMSALQKNFIETHKLMYDNTSEVTGKEDSIMEEESTSAVSEASRIALGPKRGPAVTEKEVLTCILCQEEQEVKLENNAMVLSACVQKSTALTQHRGKPVDHLGETLDPLFMDPDLAHGTYTGSCGHVMHAVCWQKYFEAVQLSSQQRIHVDLFDLESGEYLCPLCKSLCNTVIPIIPLQPQKINSENAEALAQLLTLARWIQTVLARISGYNIKHAKGEAPAVPVLFNQGMGDSTFEFHSILSFGVQSSVKYSNSIKEMVILFATTIYRIGLKVPPDELDPRVPMMTWSTCAFTIQAIENLLGDEGKPLFGALQNRQHNGLKALMQFAVAQRTTCPQVLIHKHLARLLSVILPNLQSENTPGLLSVDLFHVLVGAVLAFPSLYWDDTVDLQPSPLSSSYNHLYLFHLITMAHMLQILLTTDTDLSSGPPLAEGEEDSEEARCASAFFVEVSQHTDGLAGCGAPGWYLWLSLRNGITPYLRCAALLFHYLLGVAPPEELFANSAEGEFSALCSYLSLPTNLFLLFQEYWDTIRPLLQRWCGDPALLKSLKQKSAVVRYPRKRNSLIELPEDYSCLLNQASHFRCPRSADDERKHPVLCLFCGAILCSQNICCQEIVNGEEVGACVFHALHCGAGVCIFLKIRECRVVLVEGKARGCAYPAPYLDEYGETDPGLKRGNPLHLSRERYRKLHLVWQQHCIIEEIARSQETNQMLFGFNWQLL

Target ORF information:

RefSeq Version	XM_011239440.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin protein ligase E3 component n-recognin 1 (Ubr1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011239440.3

ORF Insert Sequence:

ATGAGAGAGA TTACTCACTT ACTTTGCATT GAGCCCATGC CACATAGTGC CATCGCCAGA 
AACCTACCTG AGAACGAAAA TAATGAAACT GGCTTAGAGA ATGTCATAAA CAAAGTGGCC 
ACATTTAAGA AACCAGGTGT GTCGGGCCAT GGAGTTTATG AATTGAAAGA TGAATCACTG 
AAAGACTTCA ATATGTACTT TTACCATTAT TCTAAAACAC AGCATAGCAA GGCTGAACAT 
ATGCAGAAGA AAAGGAGAAA ACAAGAAAAT AAAGATGAAG CATTGCCGCC GCCACCTCCT 
CCGGAGTTCT GCCCTGCTTT CAGCAAAGTA GTCAACCTGC TCAGCTGTGA TGTTATGATG 
TACATCCTCA GGACCATCTT TGAGCGGGCA GTGGACATGG AGTCTAATCT GTGGACAGAA 
GGGATGCTGC AGATGGCGTT CCATATATTG GCACTGGGCT TGCTGGAAGA GAAGCAGCAG 
CTTCAGAAAG CTCCTGAAGA GGAAGTGGCT TTTGACTTTT ACCATAAGGC TTCAAGATTG 
GGAAGTTCAG CCATGAATGC TCAGAATATA CAAATGCTCT TGGAAAAACT CAAAGGAATC 
CCCCAATTAG AAAGCCAGAA GGACATGATA ACATGGATAC TCCAGATGTT TGACACAGTG 
AAGCGATTAA GAGAAAAATC TTGTTTAGTT GTGGCAACCA CTTCAGGACT GGAGTGCGTT 
AAGAGTGAGG AGATTACTCA TGATAAAGAA AAGGCAGAAA GGAAGAGAAA AGCTGAGGCC 
GCTAGGCTTC ATCGCCAGAA GATCATGGCC CAGATGTCTG CCTTACAGAA AAACTTCATT 
GAAACCCACA AACTCATGTA TGATAATACG TCAGAAGTAA CAGGGAAGGA AGACTCCATT 
ATGGAGGAAG AGAGCACCTC AGCAGTCAGT GAGGCCTCTA GAATTGCTCT GGGCCCTAAA 
CGGGGCCCGG CTGTTACTGA AAAGGAGGTG CTGACGTGCA TCCTCTGCCA AGAAGAACAA 
GAGGTGAAAC TAGAAAATAA CGCCATGGTA TTGTCAGCAT GTGTGCAGAA ATCCACCGCC 
CTAACCCAGC ACAGAGGGAA GCCTGTGGAC CACTTAGGGG AAACACTGGA CCCTCTTTTC 
ATGGATCCAG ACTTGGCACA TGGAACTTAT ACAGGAAGCT GTGGTCATGT AATGCATGCA 
GTGTGCTGGC AGAAGTATTT TGAAGCTGTG CAGCTGAGCT CGCAGCAGCG CATTCACGTA 
GACCTGTTTG ACCTGGAGAG CGGCGAGTAC CTATGCCCGC TCTGCAAGTC TCTCTGCAAT 
ACTGTCATCC CCATCATCCC TTTGCAGCCG CAGAAGATCA ACAGTGAGAA TGCGGAGGCT 
CTTGCTCAAC TTTTGACCTT GGCCCGGTGG ATACAGACTG TCCTTGCCAG AATATCGGGT 
TATAATATAA AGCATGCTAA AGGAGAAGCC CCAGCAGTTC CTGTCTTGTT TAATCAAGGA 
ATGGGGGATT CGACTTTTGA GTTTCATTCC ATCCTGAGTT TTGGAGTTCA GTCTTCGGTG 
AAATATTCAA ATAGTATCAA GGAAATGGTC ATTCTCTTCG CCACAACAAT TTACAGAATT 
GGCCTGAAAG TGCCTCCTGA TGAACTAGAC CCACGAGTGC CCATGATGAC CTGGAGCACG 
TGTGCGTTCA CCATCCAGGC AATCGAAAAC CTGTTGGGAG ATGAAGGAAA ACCTCTATTT 
GGAGCACTTC AAAATAGACA GCATAACGGT CTGAAGGCGC TAATGCAGTT TGCAGTTGCA 
CAGAGGACTA CCTGCCCTCA GGTCCTGATA CACAAACATC TGGCTCGGCT CCTGTCAGTT 
ATTCTTCCTA ACCTGCAATC AGAAAATACA CCAGGCCTTC TGTCCGTGGA TCTCTTCCAT 
GTTCTGGTCG GCGCAGTCTT AGCGTTCCCA TCCTTGTATT GGGATGACAC CGTGGATCTG 
CAGCCGTCAC CACTTAGTTC TTCATATAAC CACCTCTATC TCTTCCATCT GATCACCATG 
GCGCACATGC TTCAGATACT CCTTACAACA GATACAGATC TGTCTTCAGG GCCGCCGCTT 
GCTGAGGGTG AAGAGGATAG TGAGGAGGCT CGCTGTGCAT CTGCTTTCTT TGTGGAAGTG 
TCGCAGCACA CAGACGGCCT CGCTGGGTGC GGTGCTCCCG GCTGGTACCT GTGGCTCTCC 
CTGAGGAACG GCATCACCCC TTACCTCCGC TGTGCTGCAC TGCTTTTCCA CTATTTACTT 
GGAGTAGCTC CGCCTGAAGA ACTGTTTGCC AATTCTGCTG AAGGAGAATT CAGTGCACTC 
TGTAGCTATC TATCTTTACC CACAAATTTG TTCCTGCTTT TCCAGGAATA TTGGGATACC 
ATAAGGCCCT TACTACAGAG GTGGTGTGGA GATCCTGCCT TACTCAAGTC TTTGAAGCAG 
AAAAGTGCTG TGGTCAGGTA CCCTAGAAAA AGAAATAGTT TGATAGAGCT TCCTGAGGAC 
TACAGCTGTC TTCTAAATCA GGCTTCTCAC TTTAGGTGTC CACGGTCTGC AGATGATGAG 
CGAAAGCATC CTGTCCTCTG TCTTTTCTGT GGGGCCATCC TGTGTTCTCA GAACATCTGT 
TGCCAAGAAA TAGTGAATGG GGAAGAGGTT GGAGCGTGCG TTTTTCATGC GCTTCATTGT 
GGTGCTGGAG TCTGCATTTT CTTAAAAATC CGAGAATGCA GGGTGGTCCT GGTGGAAGGA 
AAAGCCAGAG GCTGTGCCTA CCCAGCCCCT TACTTGGATG AATATGGAGA AACAGACCCA 
GGGCTAAAGA GAGGAAACCC ACTTCATTTA TCTCGGGAGC GGTATCGGAA GCTGCATTTG 
GTCTGGCAAC AGCACTGCAT TATAGAAGAG ATTGCTCGGA GCCAGGAGAC TAATCAGATG 
CTATTTGGAT TCAACTGGCA GTTACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ubr1 ( XM_006499165.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006499228.1 Mus musculus ubiquitin protein ligase E3 component n-recognin 1 (Ubr1), transcript variant X3, mRNA.

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CloneID	OMu30250
Clone ID Related Accession (Same CDS sequence)	XM_006499165.3
Accession Version	XM_006499165.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase UBR1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006499165.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACG AAGAGATGGA CGGCGCCGAG AGGATGGACG TCAGCCCGGA GCCTCCCCTG 
GCCCCGCAGC GGCCGGCATC GTGGTGGGAT CAGCAAGTTG ATTTCTATAC TGCTTTCTTA 
CATCATTTGG CACAATTAGT GCCAGAAATT TATTTTGCTG AGATGGACCC AGATTTGGAA 
AAGCAAGAAG AGAGTGTACA GATGTCAATA CTCACTCCTT TGGAGTGGTA CTTATTTGGA 
GAGGATCCGG ATATTTGCTT AGAGAAATTA AAACACAGTG GAGCGTTCCA GTTGTGTGGG 
AAGGTTTTCA AAAGTGGAGA AACAACATAT TCCTGTAGGG ATTGTGCAAT TGATCCAACG 
TGTGTGCTCT GTATGGACTG CTTCCAAAGT AGTGTTCATA AAAACCATCG TTACAAGATG 
CATACTTCTA CTGGAGGAGG CTTCTGTGAC TGTGGAGACA CAGAAGCGTG GAAAACTGGC 
CCTTTTTGTG TGGATCACGA GCCTGGAAGA GCAGGTACTA CAAAAGAGAG CTTACATTGC 
CCATTGAATG AAGAGGTGAT TGCTCAAGCC AGGAGAATAT TCCCTTCGGT GATAAAATAC 
ATTGTAGAAA TGACTATATG GGAAGAAGAA AAGGAATTGC CTCCTGAACT GCAGATAAGG 
GAGAAAAATG AACGATACTA TTGTGTCCTT TTCAACGATG AGCACCATTC GTATGATCAT 
GTGATCTACA GTCTGCAGAG AGCTCTAGAT TGCGAGCTTG CAGAGGCACA GCTGCACACG 
ACTGCCATCG ACAAAGAGGG TCGCCGGGCT GTCAAAGCAG GTGTGTATGC CACTTGCCAG 
GAAGCAAAGG AGGATATAAA GAGTCACTCA GAGAACGTCT CTCAGCACCC CCTCCATGTG 
GAAGTGCTGC ACTCCGTGGT TATGGCTCAC CAGAAATTCG CTCTGCGCCT TGGCTCCTGG 
ATGAACAAAA TTATGAGCTA TTCAAGTGAC TTTAGACAGA TATTTTGCCA GGCCTGCCTC 
GTAGAAGAAC CTGGCTCTGA AAATCCCTGT CTTATAAGCA GACTAATGCT TTGGGATGCA 
AAACTTTATA AAGGTGCCCG TAAGATCCTT CATGAATTGA TCTTTAGTAG TTTTTTTATG 
GAGATGGAAT ACAAAAAACT CTTTGCTATG GAATTTGTGA AGTATTATAA ACAGCTGCAG 
AAAGAGTACA TCAGCGACGA CCACGAGAGA AGCATCTCCA TAACCGCCCT GTCCGTGCAG 
ATGCTCACCG TCCCGACCTT GGCCCGGCAT CTTATTGAAG AGCAGAATGT TATTTCTGTC 
ATTACTGAAA CGCTGCTAGA AGTTTTACCT GAATACTTGG ACAGGAACAA TAAATTCAAC 
TTCCAGGGTT ATAGCCAGGA CAAACTGGGA AGAGTCTACG CAGTTATATG TGACCTAAAG 
TATATCCTGA TTAGCAAGCC TGTCATATGG ACAGAACGAT TAAGAGCGCA GTTCCTGGAA 
GGTTTCCGGT CTTTTCTGAA GATTCTTACC TGTATGCAGG GAATGGAAGA AATCAGAAGA 
CAAGTTGGAC AACACATTGA AGTGGACCCT GACTGGGAGG CTGCCATCGC TATACAGATG 
CAACTAAAGA ATATTTTGCT CATGTTCCAA GAGTGGTGTG CTTGTGATGA AGATCTCTTA 
CTGGTGGCTT ATAAAGAATG TCACAAAGCT GTAATGAGGT GCAGTACAAA TTTCATGTCC 
AGTACCAAGA CAGTAGTGCA ATTGTGCGGT CATAGTCTGG AAACCAAATC CTACAAAGTG 
TCTGAGGACC TTGTAAGCAT ACACCTGCCA CTCTCTAGAA CACTTGCTGG TCTTCATGTA 
CGTTTAAGCA GACTAGGTGC TATTTCAAGA CTGCATGAAT TTGTGCCTTT TGACGGCTTT 
CAAGTAGAGG TCCTGGTGGA GTACCCGCTG CGCTGCCTGG TCCTGGTGGC TCAGGTTGTT 
GCTGAGATGT GGCGAAGAAA CGGGCTCTCA CTCATCAGCC AGGTTTTCTA TTATCAAGAT 
GTTAAATGCA GGGAGGAAAT GTACGATAAA GATATCATCA TGCTTCAGAT TGGAGCATCT 
ATAATGGATC CCAACAAGTT CTTGTTACTG GTACTTCAGA GATATGAACT TACTGATGCT 
TTTAACAAGA CCATATCCAC AAAAGACCAG GATTTGATTA AACAGTATAA TACATTAATA 
GAAGAAATGC TTCAGGTCCT CATCTATATT GTGGGAGAAC GTTATGTACC TGGAGTGGGA 
AATGTTACCA GAGAGGAGGT TATAATGAGA GAGATTACTC ACTTACTTTG CATTGAGCCC 
ATGCCACATA GTGCCATCGC CAGAAACCTA CCTGAGAACG AAAATAATGA AACTGGCTTA 
GAGAATGTCA TAAACAAAGT GGCCACATTT AAGAAACCAG GTGTGTCGGG CCATGGAGTT 
TATGAATTGA AAGATGAATC ACTGAAAGAC TTCAATATGT ACTTTTACCA TTATTCTAAA 
ACACAGCATA GCAAGGCTGA ACATATGCAG AAGAAAAGGA GAAAACAAGA AAATAAAGAT 
GAAGCATTGC CGCCGCCACC TCCTCCGGAG TTCTGCCCTG CTTTCAGCAA AGTAGTCAAC 
CTGCTCAGCT GTGATGTTAT GATGTACATC CTCAGGACCA TCTTTGAGCG GGCAGTGGAC 
ATGGAGTCTA ATCTGTGGAC AGAAGGGATG CTGCAGATGG CGTTCCATAT ATTGGCACTG 
GGCTTGCTGG AAGAGAAGCA GCAGCTTCAG AAAGCTCCTG AAGAGGAAGT GGCTTTTGAC 
TTTTACCATA AGGCTTCAAG ATTGGGAAGT TCAGCCATGA ATGCTCAGAA TATACAAATG 
CTCTTGGAAA AACTCAAAGG AATCCCCCAA TTAGAAAGCC AGAAGGACAT GATAACATGG 
ATACTCCAGA TGTTTGACAC AGTGAAGCGA TTAAGAGAAA AATCTTGTTT AGTTGTGGCA 
ACCACTTCAG GACTGGAGTG CGTTAAGAGT GAGGAGATTA CTCATGATAA AGAAAAGGCA 
GAAAGGAAGA GAAAAGCTGA GGCCGCTAGG CTTCATCGCC AGAAGATCAT GGCCCAGATG 
TCTGCCTTAC AGAAAAACTT CATTGAAACC CACAAACTCA TGTATGATAA TACGTCAGAA 
GTAACAGGGA AGGAAGACTC CATTATGGAG GAAGAGAGCA CCTCAGCAGT CAGTGAGGCC 
TCTAGAATTG CTCTGGGCCC TAAACGGGGC CCGGCTGTTA CTGAAAAGGA GGTGCTGACG 
TGCATCCTCT GCCAAGAAGA ACAAGAGGTG AAACTAGAAA ATAACGCCAT GGTATTGTCA 
GCATGTGTGC AGAAATCCAC CGCCCTAACC CAGCACAGAG GGAAGCCTGT GGACCACTTA 
GGGGAAACAC TGGACCCTCT TTTCATGGAT CCAGACTTGG CACATGGAAC TTATACAGGA 
AGCTGTGGTC ATGTAATGCA TGCAGTGTGC TGGCAGAAGT ATTTTGAAGC TGTGCAGCTG 
AGCTCGCAGC AGCGCATTCA CGTAGACCTG TTTGACCTGG AGAGCGGCGA GTACCTATGC 
CCGCTCTGCA AGTCTCTCTG CAATACTGTC ATCCCCATCA TCCCTTTGCA GCCGCAGAAG 
ATCAACAGTG AGAATGCGGA GGCTCTTGCT CAACTTTTGA CCTTGGCCCG GTGGATACAG 
ACTGTCCTTG CCAGAATATC GGGTTATAAT ATAAAGCATG CTAAAGGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006499228.1
CDS	127..3897
Translation	MADEEMDGAERMDVSPEPPLAPQRPASWWDQQVDFYTAFLHHLAQLVPEIYFAEMDPDLEKQEESVQMSILTPLEWYLFGEDPDICLEKLKHSGAFQLCGKVFKSGETTYSCRDCAIDPTCVLCMDCFQSSVHKNHRYKMHTSTGGGFCDCGDTEAWKTGPFCVDHEPGRAGTTKESLHCPLNEEVIAQARRIFPSVIKYIVEMTIWEEEKELPPELQIREKNERYYCVLFNDEHHSYDHVIYSLQRALDCELAEAQLHTTAIDKEGRRAVKAGVYATCQEAKEDIKSHSENVSQHPLHVEVLHSVVMAHQKFALRLGSWMNKIMSYSSDFRQIFCQACLVEEPGSENPCLISRLMLWDAKLYKGARKILHELIFSSFFMEMEYKKLFAMEFVKYYKQLQKEYISDDHERSISITALSVQMLTVPTLARHLIEEQNVISVITETLLEVLPEYLDRNNKFNFQGYSQDKLGRVYAVICDLKYILISKPVIWTERLRAQFLEGFRSFLKILTCMQGMEEIRRQVGQHIEVDPDWEAAIAIQMQLKNILLMFQEWCACDEDLLLVAYKECHKAVMRCSTNFMSSTKTVVQLCGHSLETKSYKVSEDLVSIHLPLSRTLAGLHVRLSRLGAISRLHEFVPFDGFQVEVLVEYPLRCLVLVAQVVAEMWRRNGLSLISQVFYYQDVKCREEMYDKDIIMLQIGASIMDPNKFLLLVLQRYELTDAFNKTISTKDQDLIKQYNTLIEEMLQVLIYIVGERYVPGVGNVTREEVIMREITHLLCIEPMPHSAIARNLPENENNETGLENVINKVATFKKPGVSGHGVYELKDESLKDFNMYFYHYSKTQHSKAEHMQKKRRKQENKDEALPPPPPPEFCPAFSKVVNLLSCDVMMYILRTIFERAVDMESNLWTEGMLQMAFHILALGLLEEKQQLQKAPEEEVAFDFYHKASRLGSSAMNAQNIQMLLEKLKGIPQLESQKDMITWILQMFDTVKRLREKSCLVVATTSGLECVKSEEITHDKEKAERKRKAEAARLHRQKIMAQMSALQKNFIETHKLMYDNTSEVTGKEDSIMEEESTSAVSEASRIALGPKRGPAVTEKEVLTCILCQEEQEVKLENNAMVLSACVQKSTALTQHRGKPVDHLGETLDPLFMDPDLAHGTYTGSCGHVMHAVCWQKYFEAVQLSSQQRIHVDLFDLESGEYLCPLCKSLCNTVIPIIPLQPQKINSENAEALAQLLTLARWIQTVLARISGYNIKHAKG

Target ORF information:

RefSeq Version	XM_006499165.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin protein ligase E3 component n-recognin 1 (Ubr1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499165.3

ORF Insert Sequence:

ATGGCGGACG AAGAGATGGA CGGCGCCGAG AGGATGGACG TCAGCCCGGA GCCTCCCCTG 
GCCCCGCAGC GGCCGGCATC GTGGTGGGAT CAGCAAGTTG ATTTCTATAC TGCTTTCTTA 
CATCATTTGG CACAATTAGT GCCAGAAATT TATTTTGCTG AGATGGACCC AGATTTGGAA 
AAGCAAGAAG AGAGTGTACA GATGTCAATA CTCACTCCTT TGGAGTGGTA CTTATTTGGA 
GAGGATCCGG ATATTTGCTT AGAGAAATTA AAACACAGTG GAGCGTTCCA GTTGTGTGGG 
AAGGTTTTCA AAAGTGGAGA AACAACATAT TCCTGTAGGG ATTGTGCAAT TGATCCAACG 
TGTGTGCTCT GTATGGACTG CTTCCAAAGT AGTGTTCATA AAAACCATCG TTACAAGATG 
CATACTTCTA CTGGAGGAGG CTTCTGTGAC TGTGGAGACA CAGAAGCGTG GAAAACTGGC 
CCTTTTTGTG TGGATCACGA GCCTGGAAGA GCAGGTACTA CAAAAGAGAG CTTACATTGC 
CCATTGAATG AAGAGGTGAT TGCTCAAGCC AGGAGAATAT TCCCTTCGGT GATAAAATAC 
ATTGTAGAAA TGACTATATG GGAAGAAGAA AAGGAATTGC CTCCTGAACT GCAGATAAGG 
GAGAAAAATG AACGATACTA TTGTGTCCTT TTCAACGATG AGCACCATTC GTATGATCAT 
GTGATCTACA GTCTGCAGAG AGCTCTAGAT TGCGAGCTTG CAGAGGCACA GCTGCACACG 
ACTGCCATCG ACAAAGAGGG TCGCCGGGCT GTCAAAGCAG GTGTGTATGC CACTTGCCAG 
GAAGCAAAGG AGGATATAAA GAGTCACTCA GAGAACGTCT CTCAGCACCC CCTCCATGTG 
GAAGTGCTGC ACTCCGTGGT TATGGCTCAC CAGAAATTCG CTCTGCGCCT TGGCTCCTGG 
ATGAACAAAA TTATGAGCTA TTCAAGTGAC TTTAGACAGA TATTTTGCCA GGCCTGCCTC 
GTAGAAGAAC CTGGCTCTGA AAATCCCTGT CTTATAAGCA GACTAATGCT TTGGGATGCA 
AAACTTTATA AAGGTGCCCG TAAGATCCTT CATGAATTGA TCTTTAGTAG TTTTTTTATG 
GAGATGGAAT ACAAAAAACT CTTTGCTATG GAATTTGTGA AGTATTATAA ACAGCTGCAG 
AAAGAGTACA TCAGCGACGA CCACGAGAGA AGCATCTCCA TAACCGCCCT GTCCGTGCAG 
ATGCTCACCG TCCCGACCTT GGCCCGGCAT CTTATTGAAG AGCAGAATGT TATTTCTGTC 
ATTACTGAAA CGCTGCTAGA AGTTTTACCT GAATACTTGG ACAGGAACAA TAAATTCAAC 
TTCCAGGGTT ATAGCCAGGA CAAACTGGGA AGAGTCTACG CAGTTATATG TGACCTAAAG 
TATATCCTGA TTAGCAAGCC TGTCATATGG ACAGAACGAT TAAGAGCGCA GTTCCTGGAA 
GGTTTCCGGT CTTTTCTGAA GATTCTTACC TGTATGCAGG GAATGGAAGA AATCAGAAGA 
CAAGTTGGAC AACACATTGA AGTGGACCCT GACTGGGAGG CTGCCATCGC TATACAGATG 
CAACTAAAGA ATATTTTGCT CATGTTCCAA GAGTGGTGTG CTTGTGATGA AGATCTCTTA 
CTGGTGGCTT ATAAAGAATG TCACAAAGCT GTAATGAGGT GCAGTACAAA TTTCATGTCC 
AGTACCAAGA CAGTAGTGCA ATTGTGCGGT CATAGTCTGG AAACCAAATC CTACAAAGTG 
TCTGAGGACC TTGTAAGCAT ACACCTGCCA CTCTCTAGAA CACTTGCTGG TCTTCATGTA 
CGTTTAAGCA GACTAGGTGC TATTTCAAGA CTGCATGAAT TTGTGCCTTT TGACGGCTTT 
CAAGTAGAGG TCCTGGTGGA GTACCCGCTG CGCTGCCTGG TCCTGGTGGC TCAGGTTGTT 
GCTGAGATGT GGCGAAGAAA CGGGCTCTCA CTCATCAGCC AGGTTTTCTA TTATCAAGAT 
GTTAAATGCA GGGAGGAAAT GTACGATAAA GATATCATCA TGCTTCAGAT TGGAGCATCT 
ATAATGGATC CCAACAAGTT CTTGTTACTG GTACTTCAGA GATATGAACT TACTGATGCT 
TTTAACAAGA CCATATCCAC AAAAGACCAG GATTTGATTA AACAGTATAA TACATTAATA 
GAAGAAATGC TTCAGGTCCT CATCTATATT GTGGGAGAAC GTTATGTACC TGGAGTGGGA 
AATGTTACCA GAGAGGAGGT TATAATGAGA GAGATTACTC ACTTACTTTG CATTGAGCCC 
ATGCCACATA GTGCCATCGC CAGAAACCTA CCTGAGAACG AAAATAATGA AACTGGCTTA 
GAGAATGTCA TAAACAAAGT GGCCACATTT AAGAAACCAG GTGTGTCGGG CCATGGAGTT 
TATGAATTGA AAGATGAATC ACTGAAAGAC TTCAATATGT ACTTTTACCA TTATTCTAAA 
ACACAGCATA GCAAGGCTGA ACATATGCAG AAGAAAAGGA GAAAACAAGA AAATAAAGAT 
GAAGCATTGC CGCCGCCACC TCCTCCGGAG TTCTGCCCTG CTTTCAGCAA AGTAGTCAAC 
CTGCTCAGCT GTGATGTTAT GATGTACATC CTCAGGACCA TCTTTGAGCG GGCAGTGGAC 
ATGGAGTCTA ATCTGTGGAC AGAAGGGATG CTGCAGATGG CGTTCCATAT ATTGGCACTG 
GGCTTGCTGG AAGAGAAGCA GCAGCTTCAG AAAGCTCCTG AAGAGGAAGT GGCTTTTGAC 
TTTTACCATA AGGCTTCAAG ATTGGGAAGT TCAGCCATGA ATGCTCAGAA TATACAAATG 
CTCTTGGAAA AACTCAAAGG AATCCCCCAA TTAGAAAGCC AGAAGGACAT GATAACATGG 
ATACTCCAGA TGTTTGACAC AGTGAAGCGA TTAAGAGAAA AATCTTGTTT AGTTGTGGCA 
ACCACTTCAG GACTGGAGTG CGTTAAGAGT GAGGAGATTA CTCATGATAA AGAAAAGGCA 
GAAAGGAAGA GAAAAGCTGA GGCCGCTAGG CTTCATCGCC AGAAGATCAT GGCCCAGATG 
TCTGCCTTAC AGAAAAACTT CATTGAAACC CACAAACTCA TGTATGATAA TACGTCAGAA 
GTAACAGGGA AGGAAGACTC CATTATGGAG GAAGAGAGCA CCTCAGCAGT CAGTGAGGCC 
TCTAGAATTG CTCTGGGCCC TAAACGGGGC CCGGCTGTTA CTGAAAAGGA GGTGCTGACG 
TGCATCCTCT GCCAAGAAGA ACAAGAGGTG AAACTAGAAA ATAACGCCAT GGTATTGTCA 
GCATGTGTGC AGAAATCCAC CGCCCTAACC CAGCACAGAG GGAAGCCTGT GGACCACTTA 
GGGGAAACAC TGGACCCTCT TTTCATGGAT CCAGACTTGG CACATGGAAC TTATACAGGA 
AGCTGTGGTC ATGTAATGCA TGCAGTGTGC TGGCAGAAGT ATTTTGAAGC TGTGCAGCTG 
AGCTCGCAGC AGCGCATTCA CGTAGACCTG TTTGACCTGG AGAGCGGCGA GTACCTATGC 
CCGCTCTGCA AGTCTCTCTG CAATACTGTC ATCCCCATCA TCCCTTTGCA GCCGCAGAAG 
ATCAACAGTG AGAATGCGGA GGCTCTTGCT CAACTTTTGA CCTTGGCCCG GTGGATACAG 
ACTGTCCTTG CCAGAATATC GGGTTATAAT ATAAAGCATG CTAAAGGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ubr1 ( XM_006499164.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006499227.1 Mus musculus ubiquitin protein ligase E3 component n-recognin 1 (Ubr1), transcript variant X1, mRNA.

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CloneID	OMu30249
Clone ID Related Accession (Same CDS sequence)	XM_006499164.3 , XM_006499164.2
Accession Version	XM_006499164.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5271bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase UBR1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006499164.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACG AAGAGATGGA CGGCGCCGAG AGGATGGACG TCAGCCCGGA GCCTCCCCTG 
GCCCCGCAGC GGCCGGCATC GTGGTGGGAT CAGCAAGTTG ATTTCTATAC TGCTTTCTTA 
CATCATTTGG CACAATTAGT GCCAGAAATT TATTTTGCTG AGATGGACCC AGATTTGGAA 
AAGCAAGAAG AGAGTGTACA GATGTCAATA CTCACTCCTT TGGAGTGGTA CTTATTTGGA 
GAGGATCCGG ATATTTGCTT AGAGAAATTA AAACACAGTG GAGCGTTCCA GTTGTGTGGG 
AAGGTTTTCA AAAGTGGAGA AACAACATAT TCCTGTAGGG ATTGTGCAAT TGATCCAACG 
TGTGTGCTCT GTATGGACTG CTTCCAAAGT AGTGTTCATA AAAACCATCG TTACAAGATG 
CATACTTCTA CTGGAGGAGG CTTCTGTGAC TGTGGAGACA CAGAAGCGTG GAAAACTGGC 
CCTTTTTGTG TGGATCACGA GCCTGGAAGA GCAGGTACTA CAAAAGAGAG CTTACATTGC 
CCATTGAATG AAGAGGTGAT TGCTCAAGCC AGGAGAATAT TCCCTTCGGT GATAAAATAC 
ATTGTAGAAA TGACTATATG GGAAGAAGAA AAGGAATTGC CTCCTGAACT GCAGATAAGG 
GAGAAAAATG AACGATACTA TTGTGTCCTT TTCAACGATG AGCACCATTC GTATGATCAT 
GTGATCTACA GTCTGCAGAG AGCTCTAGAT TGCGAGCTTG CAGAGGCACA GCTGCACACG 
ACTGCCATCG ACAAAGAGGG TCGCCGGGCT GTCAAAGCAG GTGTGTATGC CACTTGCCAG 
GAAGCAAAGG AGGATATAAA GAGTCACTCA GAGAACGTCT CTCAGCACCC CCTCCATGTG 
GAAGTGCTGC ACTCCGTGGT TATGGCTCAC CAGAAATTCG CTCTGCGCCT TGGCTCCTGG 
ATGAACAAAA TTATGAGCTA TTCAAGTGAC TTTAGACAGA TATTTTGCCA GGCCTGCCTC 
GTAGAAGAAC CTGGCTCTGA AAATCCCTGT CTTATAAGCA GACTAATGCT TTGGGATGCA 
AAACTTTATA AAGGTGCCCG TAAGATCCTT CATGAATTGA TCTTTAGTAG TTTTTTTATG 
GAGATGGAAT ACAAAAAACT CTTTGCTATG GAATTTGTGA AGTATTATAA ACAGCTGCAG 
AAAGAGTACA TCAGCGACGA CCACGAGAGA AGCATCTCCA TAACCGCCCT GTCCGTGCAG 
ATGCTCACCG TCCCGACCTT GGCCCGGCAT CTTATTGAAG AGCAGAATGT TATTTCTGTC 
ATTACTGAAA CGCTGCTAGA AGTTTTACCT GAATACTTGG ACAGGAACAA TAAATTCAAC 
TTCCAGGGTT ATAGCCAGGA CAAACTGGGA AGAGTCTACG CAGTTATATG TGACCTAAAG 
TATATCCTGA TTAGCAAGCC TGTCATATGG ACAGAACGAT TAAGAGCGCA GTTCCTGGAA 
GGTTTCCGGT CTTTTCTGAA GATTCTTACC TGTATGCAGG GAATGGAAGA AATCAGAAGA 
CAAGTTGGAC AACACATTGA AGTGGACCCT GACTGGGAGG CTGCCATCGC TATACAGATG 
CAACTAAAGA ATATTTTGCT CATGTTCCAA GAGTGGTGTG CTTGTGATGA AGATCTCTTA 
CTGGTGGCTT ATAAAGAATG TCACAAAGCT GTAATGAGGT GCAGTACAAA TTTCATGTCC 
AGTACCAAGA CAGTAGTGCA ATTGTGCGGT CATAGTCTGG AAACCAAATC CTACAAAGTG 
TCTGAGGACC TTGTAAGCAT ACACCTGCCA CTCTCTAGAA CACTTGCTGG TCTTCATGTA 
CGTTTAAGCA GACTAGGTGC TATTTCAAGA CTGCATGAAT TTGTGCCTTT TGACGGCTTT 
CAAGTAGAGG TCCTGGTGGA GTACCCGCTG CGCTGCCTGG TCCTGGTGGC TCAGGTTGTT 
GCTGAGATGT GGCGAAGAAA CGGGCTCTCA CTCATCAGCC AGGTTTTCTA TTATCAAGAT 
GTTAAATGCA GGGAGGAAAT GTACGATAAA GATATCATCA TGCTTCAGAT TGGAGCATCT 
ATAATGGATC CCAACAAGTT CTTGTTACTG GTACTTCAGA GATATGAACT TACTGATGCT 
TTTAACAAGA CCATATCCAC AAAAGACCAG GATTTGATTA AACAGTATAA TACATTAATA 
GAAGAAATGC TTCAGGTCCT CATCTATATT GTGGGAGAAC GTTATGTACC TGGAGTGGGA 
AATGTTACCA GAGAGGAGGT TATAATGAGA GAGATTACTC ACTTACTTTG CATTGAGCCC 
ATGCCACATA GTGCCATCGC CAGAAACCTA CCTGAGAACG AAAATAATGA AACTGGCTTA 
GAGAATGTCA TAAACAAAGT GGCCACATTT AAGAAACCAG GTGTGTCGGG CCATGGAGTT 
TATGAATTGA AAGATGAATC ACTGAAAGAC TTCAATATGT ACTTTTACCA TTATTCTAAA 
ACACAGCATA GCAAGGCTGA ACATATGCAG AAGAAAAGGA GAAAACAAGA AAATAAAGAT 
GAAGCATTGC CGCCGCCACC TCCTCCGGAG TTCTGCCCTG CTTTCAGCAA AGTAGTCAAC 
CTGCTCAGCT GTGATGTTAT GATGTACATC CTCAGGACCA TCTTTGAGCG GGCAGTGGAC 
ATGGAGTCTA ATCTGTGGAC AGAAGGGATG CTGCAGATGG CGTTCCATAT ATTGGCACTG 
GGCTTGCTGG AAGAGAAGCA GCAGCTTCAG AAAGCTCCTG AAGAGGAAGT GGCTTTTGAC 
TTTTACCATA AGGCTTCAAG ATTGGGAAGT TCAGCCATGA ATGCTCAGAA TATACAAATG 
CTCTTGGAAA AACTCAAAGG AATCCCCCAA TTAGAAAGCC AGAAGGACAT GATAACATGG 
ATACTCCAGA TGTTTGACAC AGTGAAGCGA TTAAGAGAAA AATCTTGTTT AGTTGTGGCA 
ACCACTTCAG GACTGGAGTG CGTTAAGAGT GAGGAGATTA CTCATGATAA AGAAAAGGCA 
GAAAGGAAGA GAAAAGCTGA GGCCGCTAGG CTTCATCGCC AGAAGATCAT GGCCCAGATG 
TCTGCCTTAC AGAAAAACTT CATTGAAACC CACAAACTCA TGTATGATAA TACGTCAGAA 
GTAACAGGGA AGGAAGACTC CATTATGGAG GAAGAGAGCA CCTCAGCAGT CAGTGAGGCC 
TCTAGAATTG CTCTGGGCCC TAAACGGGGC CCGGCTGTTA CTGAAAAGGA GGTGCTGACG 
TGCATCCTCT GCCAAGAAGA ACAAGAGGTG AAACTAGAAA ATAACGCCAT GGTATTGTCA 
GCATGTGTGC AGAAATCCAC CGCCCTAACC CAGCACAGAG GGAAGCCTGT GGACCACTTA 
GGGGAAACAC TGGACCCTCT TTTCATGGAT CCAGACTTGG CACATGGAAC TTATACAGGA 
AGCTGTGGTC ATGTAATGCA TGCAGTGTGC TGGCAGAAGT ATTTTGAAGC TGTGCAGCTG 
AGCTCGCAGC AGCGCATTCA CGTAGACCTG TTTGACCTGG AGAGCGGCGA GTACCTATGC 
CCGCTCTGCA AGTCTCTCTG CAATACTGTC ATCCCCATCA TCCCTTTGCA GCCGCAGAAG 
ATCAACAGTG AGAATGCGGA GGCTCTTGCT CAACTTTTGA CCTTGGCCCG GTGGATACAG 
ACTGTCCTTG CCAGAATATC GGGTTATAAT ATAAAGCATG CTAAAGAAGC CCCAGCAGTT 
CCTGTCTTGT TTAATCAAGG AATGGGGGAT TCGACTTTTG AGTTTCATTC CATCCTGAGT 
TTTGGAGTTC AGTCTTCGGT GAAATATTCA AATAGTATCA AGGAAATGGT CATTCTCTTC 
GCCACAACAA TTTACAGAAT TGGCCTGAAA GTGCCTCCTG ATGAACTAGA CCCACGAGTG 
CCCATGATGA CCTGGAGCAC GTGTGCGTTC ACCATCCAGG CAATCGAAAA CCTGTTGGGA 
GATGAAGGAA AACCTCTATT TGGAGCACTT CAAAATAGAC AGCATAACGG TCTGAAGGCG 
CTAATGCAGT TTGCAGTTGC ACAGAGGACT ACCTGCCCTC AGGTCCTGAT ACACAAACAT 
CTGGCTCGGC TCCTGTCAGT TATTCTTCCT AACCTGCAAT CAGAAAATAC ACCAGGCCTT 
CTGTCCGTGG ATCTCTTCCA TGTTCTGGTC GGCGCAGTCT TAGCGTTCCC ATCCTTGTAT 
TGGGATGACA CCGTGGATCT GCAGCCGTCA CCACTTAGTT CTTCATATAA CCACCTCTAT 
CTCTTCCATC TGATCACCAT GGCGCACATG CTTCAGATAC TCCTTACAAC AGATACAGAT 
CTGTCTTCAG GGCCGCCGCT TGCTGAGGGT GAAGAGGATA GTGAGGAGGC TCGCTGTGCA 
TCTGCTTTCT TTGTGGAAGT GTCGCAGCAC ACAGACGGCC TCGCTGGGTG CGGTGCTCCC 
GGCTGGTACC TGTGGCTCTC CCTGAGGAAC GGCATCACCC CTTACCTCCG CTGTGCTGCA 
CTGCTTTTCC ACTATTTACT TGGAGTAGCT CCGCCTGAAG AACTGTTTGC CAATTCTGCT 
GAAGGAGAAT TCAGTGCACT CTGTAGCTAT CTATCTTTAC CCACAAATTT GTTCCTGCTT 
TTCCAGGAAT ATTGGGATAC CATAAGGCCC TTACTACAGA GGTGGTGTGG AGATCCTGCC 
TTACTCAAGT CTTTGAAGCA GAAAAGTGCT GTGGTCAGGT ACCCTAGAAA AAGAAATAGT 
TTGATAGAGC TTCCTGAGGA CTACAGCTGT CTTCTAAATC AGGCTTCTCA CTTTAGGTGT 
CCACGGTCTG CAGATGATGA GCGAAAGCAT CCTGTCCTCT GTCTTTTCTG TGGGGCCATC 
CTGTGTTCTC AGAACATCTG TTGCCAAGAA ATAGTGAATG GGGAAGAGGT TGGAGCGTGC 
GTTTTTCATG CGCTTCATTG TGGTGCTGGA GTCTGCATTT TCTTAAAAAT CCGAGAATGC 
AGGGTGGTCC TGGTGGAAGG AAAAGCCAGA GGCTGTGCCT ACCCAGCCCC TTACTTGGAT 
GAATATGGAG AAACAGACCC AGGGCTAAAG AGAGGAAACC CACTTCATTT ATCTCGGGAG 
CGGTATCGGA AGCTGCATTT GGTCTGGCAA CAGCACTGCA TTATAGAAGA GATTGCTCGG 
AGCCAGGAGA CTAATCAGAT GCTATTTGGA TTCAACTGGC AGTTACTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006499227.1
CDS	159..5429
Translation	MADEEMDGAERMDVSPEPPLAPQRPASWWDQQVDFYTAFLHHLAQLVPEIYFAEMDPDLEKQEESVQMSILTPLEWYLFGEDPDICLEKLKHSGAFQLCGKVFKSGETTYSCRDCAIDPTCVLCMDCFQSSVHKNHRYKMHTSTGGGFCDCGDTEAWKTGPFCVDHEPGRAGTTKESLHCPLNEEVIAQARRIFPSVIKYIVEMTIWEEEKELPPELQIREKNERYYCVLFNDEHHSYDHVIYSLQRALDCELAEAQLHTTAIDKEGRRAVKAGVYATCQEAKEDIKSHSENVSQHPLHVEVLHSVVMAHQKFALRLGSWMNKIMSYSSDFRQIFCQACLVEEPGSENPCLISRLMLWDAKLYKGARKILHELIFSSFFMEMEYKKLFAMEFVKYYKQLQKEYISDDHERSISITALSVQMLTVPTLARHLIEEQNVISVITETLLEVLPEYLDRNNKFNFQGYSQDKLGRVYAVICDLKYILISKPVIWTERLRAQFLEGFRSFLKILTCMQGMEEIRRQVGQHIEVDPDWEAAIAIQMQLKNILLMFQEWCACDEDLLLVAYKECHKAVMRCSTNFMSSTKTVVQLCGHSLETKSYKVSEDLVSIHLPLSRTLAGLHVRLSRLGAISRLHEFVPFDGFQVEVLVEYPLRCLVLVAQVVAEMWRRNGLSLISQVFYYQDVKCREEMYDKDIIMLQIGASIMDPNKFLLLVLQRYELTDAFNKTISTKDQDLIKQYNTLIEEMLQVLIYIVGERYVPGVGNVTREEVIMREITHLLCIEPMPHSAIARNLPENENNETGLENVINKVATFKKPGVSGHGVYELKDESLKDFNMYFYHYSKTQHSKAEHMQKKRRKQENKDEALPPPPPPEFCPAFSKVVNLLSCDVMMYILRTIFERAVDMESNLWTEGMLQMAFHILALGLLEEKQQLQKAPEEEVAFDFYHKASRLGSSAMNAQNIQMLLEKLKGIPQLESQKDMITWILQMFDTVKRLREKSCLVVATTSGLECVKSEEITHDKEKAERKRKAEAARLHRQKIMAQMSALQKNFIETHKLMYDNTSEVTGKEDSIMEEESTSAVSEASRIALGPKRGPAVTEKEVLTCILCQEEQEVKLENNAMVLSACVQKSTALTQHRGKPVDHLGETLDPLFMDPDLAHGTYTGSCGHVMHAVCWQKYFEAVQLSSQQRIHVDLFDLESGEYLCPLCKSLCNTVIPIIPLQPQKINSENAEALAQLLTLARWIQTVLARISGYNIKHAKEAPAVPVLFNQGMGDSTFEFHSILSFGVQSSVKYSNSIKEMVILFATTIYRIGLKVPPDELDPRVPMMTWSTCAFTIQAIENLLGDEGKPLFGALQNRQHNGLKALMQFAVAQRTTCPQVLIHKHLARLLSVILPNLQSENTPGLLSVDLFHVLVGAVLAFPSLYWDDTVDLQPSPLSSSYNHLYLFHLITMAHMLQILLTTDTDLSSGPPLAEGEEDSEEARCASAFFVEVSQHTDGLAGCGAPGWYLWLSLRNGITPYLRCAALLFHYLLGVAPPEELFANSAEGEFSALCSYLSLPTNLFLLFQEYWDTIRPLLQRWCGDPALLKSLKQKSAVVRYPRKRNSLIELPEDYSCLLNQASHFRCPRSADDERKHPVLCLFCGAILCSQNICCQEIVNGEEVGACVFHALHCGAGVCIFLKIRECRVVLVEGKARGCAYPAPYLDEYGETDPGLKRGNPLHLSRERYRKLHLVWQQHCIIEEIARSQETNQMLFGFNWQLL

Target ORF information:

RefSeq Version	XM_006499164.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin protein ligase E3 component n-recognin 1 (Ubr1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499164.3

ORF Insert Sequence:

ATGGCGGACG AAGAGATGGA CGGCGCCGAG AGGATGGACG TCAGCCCGGA GCCTCCCCTG 
GCCCCGCAGC GGCCGGCATC GTGGTGGGAT CAGCAAGTTG ATTTCTATAC TGCTTTCTTA 
CATCATTTGG CACAATTAGT GCCAGAAATT TATTTTGCTG AGATGGACCC AGATTTGGAA 
AAGCAAGAAG AGAGTGTACA GATGTCAATA CTCACTCCTT TGGAGTGGTA CTTATTTGGA 
GAGGATCCGG ATATTTGCTT AGAGAAATTA AAACACAGTG GAGCGTTCCA GTTGTGTGGG 
AAGGTTTTCA AAAGTGGAGA AACAACATAT TCCTGTAGGG ATTGTGCAAT TGATCCAACG 
TGTGTGCTCT GTATGGACTG CTTCCAAAGT AGTGTTCATA AAAACCATCG TTACAAGATG 
CATACTTCTA CTGGAGGAGG CTTCTGTGAC TGTGGAGACA CAGAAGCGTG GAAAACTGGC 
CCTTTTTGTG TGGATCACGA GCCTGGAAGA GCAGGTACTA CAAAAGAGAG CTTACATTGC 
CCATTGAATG AAGAGGTGAT TGCTCAAGCC AGGAGAATAT TCCCTTCGGT GATAAAATAC 
ATTGTAGAAA TGACTATATG GGAAGAAGAA AAGGAATTGC CTCCTGAACT GCAGATAAGG 
GAGAAAAATG AACGATACTA TTGTGTCCTT TTCAACGATG AGCACCATTC GTATGATCAT 
GTGATCTACA GTCTGCAGAG AGCTCTAGAT TGCGAGCTTG CAGAGGCACA GCTGCACACG 
ACTGCCATCG ACAAAGAGGG TCGCCGGGCT GTCAAAGCAG GTGTGTATGC CACTTGCCAG 
GAAGCAAAGG AGGATATAAA GAGTCACTCA GAGAACGTCT CTCAGCACCC CCTCCATGTG 
GAAGTGCTGC ACTCCGTGGT TATGGCTCAC CAGAAATTCG CTCTGCGCCT TGGCTCCTGG 
ATGAACAAAA TTATGAGCTA TTCAAGTGAC TTTAGACAGA TATTTTGCCA GGCCTGCCTC 
GTAGAAGAAC CTGGCTCTGA AAATCCCTGT CTTATAAGCA GACTAATGCT TTGGGATGCA 
AAACTTTATA AAGGTGCCCG TAAGATCCTT CATGAATTGA TCTTTAGTAG TTTTTTTATG 
GAGATGGAAT ACAAAAAACT CTTTGCTATG GAATTTGTGA AGTATTATAA ACAGCTGCAG 
AAAGAGTACA TCAGCGACGA CCACGAGAGA AGCATCTCCA TAACCGCCCT GTCCGTGCAG 
ATGCTCACCG TCCCGACCTT GGCCCGGCAT CTTATTGAAG AGCAGAATGT TATTTCTGTC 
ATTACTGAAA CGCTGCTAGA AGTTTTACCT GAATACTTGG ACAGGAACAA TAAATTCAAC 
TTCCAGGGTT ATAGCCAGGA CAAACTGGGA AGAGTCTACG CAGTTATATG TGACCTAAAG 
TATATCCTGA TTAGCAAGCC TGTCATATGG ACAGAACGAT TAAGAGCGCA GTTCCTGGAA 
GGTTTCCGGT CTTTTCTGAA GATTCTTACC TGTATGCAGG GAATGGAAGA AATCAGAAGA 
CAAGTTGGAC AACACATTGA AGTGGACCCT GACTGGGAGG CTGCCATCGC TATACAGATG 
CAACTAAAGA ATATTTTGCT CATGTTCCAA GAGTGGTGTG CTTGTGATGA AGATCTCTTA 
CTGGTGGCTT ATAAAGAATG TCACAAAGCT GTAATGAGGT GCAGTACAAA TTTCATGTCC 
AGTACCAAGA CAGTAGTGCA ATTGTGCGGT CATAGTCTGG AAACCAAATC CTACAAAGTG 
TCTGAGGACC TTGTAAGCAT ACACCTGCCA CTCTCTAGAA CACTTGCTGG TCTTCATGTA 
CGTTTAAGCA GACTAGGTGC TATTTCAAGA CTGCATGAAT TTGTGCCTTT TGACGGCTTT 
CAAGTAGAGG TCCTGGTGGA GTACCCGCTG CGCTGCCTGG TCCTGGTGGC TCAGGTTGTT 
GCTGAGATGT GGCGAAGAAA CGGGCTCTCA CTCATCAGCC AGGTTTTCTA TTATCAAGAT 
GTTAAATGCA GGGAGGAAAT GTACGATAAA GATATCATCA TGCTTCAGAT TGGAGCATCT 
ATAATGGATC CCAACAAGTT CTTGTTACTG GTACTTCAGA GATATGAACT TACTGATGCT 
TTTAACAAGA CCATATCCAC AAAAGACCAG GATTTGATTA AACAGTATAA TACATTAATA 
GAAGAAATGC TTCAGGTCCT CATCTATATT GTGGGAGAAC GTTATGTACC TGGAGTGGGA 
AATGTTACCA GAGAGGAGGT TATAATGAGA GAGATTACTC ACTTACTTTG CATTGAGCCC 
ATGCCACATA GTGCCATCGC CAGAAACCTA CCTGAGAACG AAAATAATGA AACTGGCTTA 
GAGAATGTCA TAAACAAAGT GGCCACATTT AAGAAACCAG GTGTGTCGGG CCATGGAGTT 
TATGAATTGA AAGATGAATC ACTGAAAGAC TTCAATATGT ACTTTTACCA TTATTCTAAA 
ACACAGCATA GCAAGGCTGA ACATATGCAG AAGAAAAGGA GAAAACAAGA AAATAAAGAT 
GAAGCATTGC CGCCGCCACC TCCTCCGGAG TTCTGCCCTG CTTTCAGCAA AGTAGTCAAC 
CTGCTCAGCT GTGATGTTAT GATGTACATC CTCAGGACCA TCTTTGAGCG GGCAGTGGAC 
ATGGAGTCTA ATCTGTGGAC AGAAGGGATG CTGCAGATGG CGTTCCATAT ATTGGCACTG 
GGCTTGCTGG AAGAGAAGCA GCAGCTTCAG AAAGCTCCTG AAGAGGAAGT GGCTTTTGAC 
TTTTACCATA AGGCTTCAAG ATTGGGAAGT TCAGCCATGA ATGCTCAGAA TATACAAATG 
CTCTTGGAAA AACTCAAAGG AATCCCCCAA TTAGAAAGCC AGAAGGACAT GATAACATGG 
ATACTCCAGA TGTTTGACAC AGTGAAGCGA TTAAGAGAAA AATCTTGTTT AGTTGTGGCA 
ACCACTTCAG GACTGGAGTG CGTTAAGAGT GAGGAGATTA CTCATGATAA AGAAAAGGCA 
GAAAGGAAGA GAAAAGCTGA GGCCGCTAGG CTTCATCGCC AGAAGATCAT GGCCCAGATG 
TCTGCCTTAC AGAAAAACTT CATTGAAACC CACAAACTCA TGTATGATAA TACGTCAGAA 
GTAACAGGGA AGGAAGACTC CATTATGGAG GAAGAGAGCA CCTCAGCAGT CAGTGAGGCC 
TCTAGAATTG CTCTGGGCCC TAAACGGGGC CCGGCTGTTA CTGAAAAGGA GGTGCTGACG 
TGCATCCTCT GCCAAGAAGA ACAAGAGGTG AAACTAGAAA ATAACGCCAT GGTATTGTCA 
GCATGTGTGC AGAAATCCAC CGCCCTAACC CAGCACAGAG GGAAGCCTGT GGACCACTTA 
GGGGAAACAC TGGACCCTCT TTTCATGGAT CCAGACTTGG CACATGGAAC TTATACAGGA 
AGCTGTGGTC ATGTAATGCA TGCAGTGTGC TGGCAGAAGT ATTTTGAAGC TGTGCAGCTG 
AGCTCGCAGC AGCGCATTCA CGTAGACCTG TTTGACCTGG AGAGCGGCGA GTACCTATGC 
CCGCTCTGCA AGTCTCTCTG CAATACTGTC ATCCCCATCA TCCCTTTGCA GCCGCAGAAG 
ATCAACAGTG AGAATGCGGA GGCTCTTGCT CAACTTTTGA CCTTGGCCCG GTGGATACAG 
ACTGTCCTTG CCAGAATATC GGGTTATAAT ATAAAGCATG CTAAAGAAGC CCCAGCAGTT 
CCTGTCTTGT TTAATCAAGG AATGGGGGAT TCGACTTTTG AGTTTCATTC CATCCTGAGT 
TTTGGAGTTC AGTCTTCGGT GAAATATTCA AATAGTATCA AGGAAATGGT CATTCTCTTC 
GCCACAACAA TTTACAGAAT TGGCCTGAAA GTGCCTCCTG ATGAACTAGA CCCACGAGTG 
CCCATGATGA CCTGGAGCAC GTGTGCGTTC ACCATCCAGG CAATCGAAAA CCTGTTGGGA 
GATGAAGGAA AACCTCTATT TGGAGCACTT CAAAATAGAC AGCATAACGG TCTGAAGGCG 
CTAATGCAGT TTGCAGTTGC ACAGAGGACT ACCTGCCCTC AGGTCCTGAT ACACAAACAT 
CTGGCTCGGC TCCTGTCAGT TATTCTTCCT AACCTGCAAT CAGAAAATAC ACCAGGCCTT 
CTGTCCGTGG ATCTCTTCCA TGTTCTGGTC GGCGCAGTCT TAGCGTTCCC ATCCTTGTAT 
TGGGATGACA CCGTGGATCT GCAGCCGTCA CCACTTAGTT CTTCATATAA CCACCTCTAT 
CTCTTCCATC TGATCACCAT GGCGCACATG CTTCAGATAC TCCTTACAAC AGATACAGAT 
CTGTCTTCAG GGCCGCCGCT TGCTGAGGGT GAAGAGGATA GTGAGGAGGC TCGCTGTGCA 
TCTGCTTTCT TTGTGGAAGT GTCGCAGCAC ACAGACGGCC TCGCTGGGTG CGGTGCTCCC 
GGCTGGTACC TGTGGCTCTC CCTGAGGAAC GGCATCACCC CTTACCTCCG CTGTGCTGCA 
CTGCTTTTCC ACTATTTACT TGGAGTAGCT CCGCCTGAAG AACTGTTTGC CAATTCTGCT 
GAAGGAGAAT TCAGTGCACT CTGTAGCTAT CTATCTTTAC CCACAAATTT GTTCCTGCTT 
TTCCAGGAAT ATTGGGATAC CATAAGGCCC TTACTACAGA GGTGGTGTGG AGATCCTGCC 
TTACTCAAGT CTTTGAAGCA GAAAAGTGCT GTGGTCAGGT ACCCTAGAAA AAGAAATAGT 
TTGATAGAGC TTCCTGAGGA CTACAGCTGT CTTCTAAATC AGGCTTCTCA CTTTAGGTGT 
CCACGGTCTG CAGATGATGA GCGAAAGCAT CCTGTCCTCT GTCTTTTCTG TGGGGCCATC 
CTGTGTTCTC AGAACATCTG TTGCCAAGAA ATAGTGAATG GGGAAGAGGT TGGAGCGTGC 
GTTTTTCATG CGCTTCATTG TGGTGCTGGA GTCTGCATTT TCTTAAAAAT CCGAGAATGC 
AGGGTGGTCC TGGTGGAAGG AAAAGCCAGA GGCTGTGCCT ACCCAGCCCC TTACTTGGAT 
GAATATGGAG AAACAGACCC AGGGCTAAAG AGAGGAAACC CACTTCATTT ATCTCGGGAG 
CGGTATCGGA AGCTGCATTT GGTCTGGCAA CAGCACTGCA TTATAGAAGA GATTGCTCGG 
AGCCAGGAGA CTAATCAGAT GCTATTTGGA TTCAACTGGC AGTTACTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ubr1 ( NM_009461.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033487.2 Mus musculus ubiquitin protein ligase E3 component n-recognin 1 (Ubr1), mRNA.

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CloneID	OMu04208
Clone ID Related Accession (Same CDS sequence)	NM_009461.2
Accession Version	NM_009461.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-22
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase UBR1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL844548.7 and AL935168.11. On Jul 27, 2007 this sequence version replaced NM_009461.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF061555.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGACG AAGAGATGGA CGGCGCCGAG AGGATGGACG TCAGCCCGGA GCCTCCCCTG 
GCCCCGCAGC GGCCGGCATC GTGGTGGGAT CAGCAAGTTG ATTTCTATAC TGCTTTCTTA 
CATCATTTGG CACAATTAGT GCCAGAAATT TATTTTGCTG AGATGGACCC AGATTTGGAA 
AAGCAAGAAG AGAGTGTACA GATGTCAATA CTCACTCCTT TGGAGTGGTA CTTATTTGGA 
GAGGATCCGG ATATTTGCTT AGAGAAATTA AAACACAGTG GAGCGTTCCA GTTGTGTGGG 
AAGGTTTTCA AAAGTGGAGA AACAACATAT TCCTGTAGGG ATTGTGCAAT TGATCCAACG 
TGTGTGCTCT GTATGGACTG CTTCCAAAGT AGTGTTCATA AAAACCATCG TTACAAGATG 
CATACTTCTA CTGGAGGAGG CTTCTGTGAC TGTGGAGACA CAGAAGCGTG GAAAACTGGC 
CCTTTTTGTG TGGATCACGA GCCTGGAAGA GCAGGTACTA CAAAAGAGAG CTTACATTGC 
CCATTGAATG AAGAGGTGAT TGCTCAAGCC AGGAGAATAT TCCCTTCGGT GATAAAATAC 
ATTGTAGAAA TGACTATATG GGAAGAAGAA AAGGAATTGC CTCCTGAACT GCAGATAAGG 
GAGAAAAATG AACGATACTA TTGTGTCCTT TTCAACGATG AGCACCATTC GTATGATCAT 
GTGATCTACA GTCTGCAGAG AGCTCTAGAT TGCGAGCTTG CAGAGGCACA GCTGCACACG 
ACTGCCATCG ACAAAGAGGG TCGCCGGGCT GTCAAAGCAG GTGTGTATGC CACTTGCCAG 
GAAGCAAAGG AGGATATAAA GAGTCACTCA GAGAACGTCT CTCAGCACCC CCTCCATGTG 
GAAGTGCTGC ACTCCGTGGT TATGGCTCAC CAGAAATTCG CTCTGCGCCT TGGCTCCTGG 
ATGAACAAAA TTATGAGCTA TTCAAGTGAC TTTAGACAGA TATTTTGCCA GGCCTGCCTC 
GTAGAAGAAC CTGGCTCTGA AAATCCCTGT CTTATAAGCA GACTAATGCT TTGGGATGCA 
AAACTTTATA AAGGTGCCCG TAAGATCCTT CATGAATTGA TCTTTAGTAG TTTTTTTATG 
GAGATGGAAT ACAAAAAACT CTTTGCTATG GAATTTGTGA AGTATTATAA ACAGCTGCAG 
AAAGAGTACA TCAGCGACGA CCACGAGAGA AGCATCTCCA TAACCGCCCT GTCCGTGCAG 
ATGCTCACCG TCCCGACCTT GGCCCGGCAT CTTATTGAAG AGCAGAATGT TATTTCTGTC 
ATTACTGAAA CGCTGCTAGA AGTTTTACCT GAATACTTGG ACAGGAACAA TAAATTCAAC 
TTCCAGGGTT ATAGCCAGGA CAAACTGGGA AGAGTCTACG CAGTTATATG TGACCTAAAG 
TATATCCTGA TTAGCAAGCC TGTCATATGG ACAGAACGAT TAAGAGCGCA GTTCCTGGAA 
GGTTTCCGGT CTTTTCTGAA GATTCTTACC TGTATGCAGG GAATGGAAGA AATCAGAAGA 
CAAGTTGGAC AACACATTGA AGTGGACCCT GACTGGGAGG CTGCCATCGC TATACAGATG 
CAACTAAAGA ATATTTTGCT CATGTTCCAA GAGTGGTGTG CTTGTGATGA AGATCTCTTA 
CTGGTGGCTT ATAAAGAATG TCACAAAGCT GTAATGAGGT GCAGTACAAA TTTCATGTCC 
AGTACCAAGA CAGTAGTGCA ATTGTGCGGT CATAGTCTGG AAACCAAATC CTACAAAGTG 
TCTGAGGACC TTGTAAGCAT ACACCTGCCA CTCTCTAGAA CACTTGCTGG TCTTCATGTA 
CGTTTAAGCA GACTAGGTGC TATTTCAAGA CTGCATGAAT TTGTGCCTTT TGACGGCTTT 
CAAGTAGAGG TCCTGGTGGA GTACCCGCTG CGCTGCCTGG TCCTGGTGGC TCAGGTTGTT 
GCTGAGATGT GGCGAAGAAA CGGGCTCTCA CTCATCAGCC AGGTTTTCTA TTATCAAGAT 
GTTAAATGCA GGGAGGAAAT GTACGATAAA GATATCATCA TGCTTCAGAT TGGAGCATCT 
ATAATGGATC CCAACAAGTT CTTGTTACTG GTACTTCAGA GATATGAACT TACTGATGCT 
TTTAACAAGA CCATATCCAC AAAAGACCAG GATTTGATTA AACAGTATAA TACATTAATA 
GAAGAAATGC TTCAGGTCCT CATCTATATT GTGGGAGAAC GTTATGTACC TGGAGTGGGA 
AATGTTACCA GAGAGGAGGT TATAATGAGA GAGATTACTC ACTTACTTTG CATTGAGCCC 
ATGCCACATA GTGCCATCGC CAGAAACCTA CCTGAGAACG AAAATAATGA AACTGGCTTA 
GAGAATGTCA TAAACAAAGT GGCCACATTT AAGAAACCAG GTGTGTCGGG CCATGGAGTT 
TATGAATTGA AAGATGAATC ACTGAAAGAC TTCAATATGT ACTTTTACCA TTATTCTAAA 
ACACAGCATA GCAAGGCTGA ACATATGCAG AAGAAAAGGA GAAAACAAGA AAATAAAGAT 
GAAGCATTGC CGCCGCCACC TCCTCCGGAG TTCTGCCCTG CTTTCAGCAA AGTAGTCAAC 
CTGCTCAGCT GTGATGTTAT GATGTACATC CTCAGGACCA TCTTTGAGCG GGCAGTGGAC 
ATGGAGTCTA ATCTGTGGAC AGAAGGGATG CTGCAGATGG CGTTCCATAT ATTGGCACTG 
GGCTTGCTGG AAGAGAAGCA GCAGCTTCAG AAAGCTCCTG AAGAGGAAGT GGCTTTTGAC 
TTTTACCATA AGGCTTCAAG ATTGGGAAGT TCAGCCATGA ATGCTCAGAA TATACAAATG 
CTCTTGGAAA AACTCAAAGG AATCCCCCAA TTAGAAAGCC AGAAGGACAT GATAACATGG 
ATACTCCAGA TGTTTGACAC AGTGAAGCGA TTAAGAGAAA AATCTTGTTT AGTTGTGGCA 
ACCACTTCAG GACTGGAGTG CGTTAAGAGT GAGGAGATTA CTCATGATAA AGAAAAGGCA 
GAAAGGAAGA GAAAAGCTGA GGCCGCTAGG CTTCATCGCC AGAAGATCAT GGCCCAGATG 
TCTGCCTTAC AGAAAAACTT CATTGAAACC CACAAACTCA TGTATGATAA TACGTCAGAA 
GTAACAGGGA AGGAAGACTC CATTATGGAG GAAGAGAGCA CCTCAGCAGT CAGTGAGGCC 
TCTAGAATTG CTCTGGGCCC TAAACGGGGC CCGGCTGTTA CTGAAAAGGA GGTGCTGACG 
TGCATCCTCT GCCAAGAAGA ACAAGAGGTG AAACTAGAAA ATAACGCCAT GGTATTGTCA 
GCATGTGTGC AGAAATCCAC CGCCCTAACC CAGCACAGAG GGAAGCCTGT GGACCACTTA 
GGGGAAACAC TGGACCCTCT TTTCATGGAT CCAGACTTGG CACATGGAAC TTATACAGGA 
AGCTGTGGTC ATGTAATGCA TGCAGTGTGC TGGCAGAAGT ATTTTGAAGC TGTGCAGCTG 
AGCTCGCAGC AGCGCATTCA CGTAGACCTG TTTGACCTGG AGAGCGGCGA GTACCTATGC 
CCGCTCTGCA AGTCTCTCTG CAATACTGTC ATCCCCATCA TCCCTTTGCA GCCGCAGAAG 
ATCAACAGTG AGAATGCGGA GGCTCTTGCT CAACTTTTGA CCTTGGCCCG GTGGATACAG 
ACTGTCCTTG CCAGAATATC GGGTTATAAT ATAAAGCATG CTAAAGGAGA AGCCCCAGCA 
GTTCCTGTCT TGTTTAATCA AGGAATGGGG GATTCGACTT TTGAGTTTCA TTCCATCCTG 
AGTTTTGGAG TTCAGTCTTC GGTGAAATAT TCAAATAGTA TCAAGGAAAT GGTCATTCTC 
TTCGCCACAA CAATTTACAG AATTGGCCTG AAAGTGCCTC CTGATGAACT AGACCCACGA 
GTGCCCATGA TGACCTGGAG CACGTGTGCG TTCACCATCC AGGCAATCGA AAACCTGTTG 
GGAGATGAAG GAAAACCTCT ATTTGGAGCA CTTCAAAATA GACAGCATAA CGGTCTGAAG 
GCGCTAATGC AGTTTGCAGT TGCACAGAGG ACTACCTGCC CTCAGGTCCT GATACACAAA 
CATCTGGCTC GGCTCCTGTC AGTTATTCTT CCTAACCTGC AATCAGAAAA TACACCAGGC 
CTTCTGTCCG TGGATCTCTT CCATGTTCTG GTCGGCGCAG TCTTAGCGTT CCCATCCTTG 
TATTGGGATG ACACCGTGGA TCTGCAGCCG TCACCACTTA GTTCTTCATA TAACCACCTC 
TATCTCTTCC ATCTGATCAC CATGGCGCAC ATGCTTCAGA TACTCCTTAC AACAGATACA 
GATCTGTCTT CAGGGCCGCC GCTTGCTGAG GGTGAAGAGG ATAGTGAGGA GGCTCGCTGT 
GCATCTGCTT TCTTTGTGGA AGTGTCGCAG CACACAGACG GCCTCGCTGG GTGCGGTGCT 
CCCGGCTGGT ACCTGTGGCT CTCCCTGAGG AACGGCATCA CCCCTTACCT CCGCTGTGCT 
GCACTGCTTT TCCACTATTT ACTTGGAGTA GCTCCGCCTG AAGAACTGTT TGCCAATTCT 
GCTGAAGGAG AATTCAGTGC ACTCTGTAGC TATCTATCTT TACCCACAAA TTTGTTCCTG 
CTTTTCCAGG AATATTGGGA TACCATAAGG CCCTTACTAC AGAGGTGGTG TGGAGATCCT 
GCCTTACTCA AGTCTTTGAA GCAGAAAAGT GCTGTGGTCA GGTACCCTAG AAAAAGAAAT 
AGTTTGATAG AGCTTCCTGA GGACTACAGC TGTCTTCTAA ATCAGGCTTC TCACTTTAGG 
TGTCCACGGT CTGCAGATGA TGAGCGAAAG CATCCTGTCC TCTGTCTTTT CTGTGGGGCC 
ATCCTGTGTT CTCAGAACAT CTGTTGCCAA GAAATAGTGA ATGGGGAAGA GGTTGGAGCG 
TGCGTTTTTC ATGCGCTTCA TTGTGGTGCT GGAGTCTGCA TTTTCTTAAA AATCCGAGAA 
TGCAGGGTGG TCCTGGTGGA AGGAAAAGCC AGAGGCTGTG CCTACCCAGC CCCTTACTTG 
GATGAATATG GAGAAACAGA CCCAGGGCTA AAGAGAGGAA ACCCACTTCA TTTATCTCGG 
GAGCGGTATC GGAAGCTGCA TTTGGTCTGG CAACAGCACT GCATTATAGA AGAGATTGCT 
CGGAGCCAGG AGACTAATCA GATGCTATTT GGATTCAACT GGCAGTTACT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033487.2
CDS	114..5387
Translation	MADEEMDGAERMDVSPEPPLAPQRPASWWDQQVDFYTAFLHHLAQLVPEIYFAEMDPDLEKQEESVQMSILTPLEWYLFGEDPDICLEKLKHSGAFQLCGKVFKSGETTYSCRDCAIDPTCVLCMDCFQSSVHKNHRYKMHTSTGGGFCDCGDTEAWKTGPFCVDHEPGRAGTTKESLHCPLNEEVIAQARRIFPSVIKYIVEMTIWEEEKELPPELQIREKNERYYCVLFNDEHHSYDHVIYSLQRALDCELAEAQLHTTAIDKEGRRAVKAGVYATCQEAKEDIKSHSENVSQHPLHVEVLHSVVMAHQKFALRLGSWMNKIMSYSSDFRQIFCQACLVEEPGSENPCLISRLMLWDAKLYKGARKILHELIFSSFFMEMEYKKLFAMEFVKYYKQLQKEYISDDHERSISITALSVQMLTVPTLARHLIEEQNVISVITETLLEVLPEYLDRNNKFNFQGYSQDKLGRVYAVICDLKYILISKPVIWTERLRAQFLEGFRSFLKILTCMQGMEEIRRQVGQHIEVDPDWEAAIAIQMQLKNILLMFQEWCACDEDLLLVAYKECHKAVMRCSTNFMSSTKTVVQLCGHSLETKSYKVSEDLVSIHLPLSRTLAGLHVRLSRLGAISRLHEFVPFDGFQVEVLVEYPLRCLVLVAQVVAEMWRRNGLSLISQVFYYQDVKCREEMYDKDIIMLQIGASIMDPNKFLLLVLQRYELTDAFNKTISTKDQDLIKQYNTLIEEMLQVLIYIVGERYVPGVGNVTREEVIMREITHLLCIEPMPHSAIARNLPENENNETGLENVINKVATFKKPGVSGHGVYELKDESLKDFNMYFYHYSKTQHSKAEHMQKKRRKQENKDEALPPPPPPEFCPAFSKVVNLLSCDVMMYILRTIFERAVDMESNLWTEGMLQMAFHILALGLLEEKQQLQKAPEEEVAFDFYHKASRLGSSAMNAQNIQMLLEKLKGIPQLESQKDMITWILQMFDTVKRLREKSCLVVATTSGLECVKSEEITHDKEKAERKRKAEAARLHRQKIMAQMSALQKNFIETHKLMYDNTSEVTGKEDSIMEEESTSAVSEASRIALGPKRGPAVTEKEVLTCILCQEEQEVKLENNAMVLSACVQKSTALTQHRGKPVDHLGETLDPLFMDPDLAHGTYTGSCGHVMHAVCWQKYFEAVQLSSQQRIHVDLFDLESGEYLCPLCKSLCNTVIPIIPLQPQKINSENAEALAQLLTLARWIQTVLARISGYNIKHAKGEAPAVPVLFNQGMGDSTFEFHSILSFGVQSSVKYSNSIKEMVILFATTIYRIGLKVPPDELDPRVPMMTWSTCAFTIQAIENLLGDEGKPLFGALQNRQHNGLKALMQFAVAQRTTCPQVLIHKHLARLLSVILPNLQSENTPGLLSVDLFHVLVGAVLAFPSLYWDDTVDLQPSPLSSSYNHLYLFHLITMAHMLQILLTTDTDLSSGPPLAEGEEDSEEARCASAFFVEVSQHTDGLAGCGAPGWYLWLSLRNGITPYLRCAALLFHYLLGVAPPEELFANSAEGEFSALCSYLSLPTNLFLLFQEYWDTIRPLLQRWCGDPALLKSLKQKSAVVRYPRKRNSLIELPEDYSCLLNQASHFRCPRSADDERKHPVLCLFCGAILCSQNICCQEIVNGEEVGACVFHALHCGAGVCIFLKIRECRVVLVEGKARGCAYPAPYLDEYGETDPGLKRGNPLHLSRERYRKLHLVWQQHCIIEEIARSQETNQMLFGFNWQLL

Target ORF information:

RefSeq Version	NM_009461.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin protein ligase E3 component n-recognin 1 (Ubr1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009461.2

ORF Insert Sequence:

ATGGCGGACG AAGAGATGGA CGGCGCCGAG AGGATGGACG TCAGCCCGGA GCCTCCCCTG 
GCCCCGCAGC GGCCGGCATC GTGGTGGGAT CAGCAAGTTG ATTTCTATAC TGCTTTCTTA 
CATCATTTGG CACAATTAGT GCCAGAAATT TATTTTGCTG AGATGGACCC AGATTTGGAA 
AAGCAAGAAG AGAGTGTACA GATGTCAATA CTCACTCCTT TGGAGTGGTA CTTATTTGGA 
GAGGATCCGG ATATTTGCTT AGAGAAATTA AAACACAGTG GAGCGTTCCA GTTGTGTGGG 
AAGGTTTTCA AAAGTGGAGA AACAACATAT TCCTGTAGGG ATTGTGCAAT TGATCCAACG 
TGTGTGCTCT GTATGGACTG CTTCCAAAGT AGTGTTCATA AAAACCATCG TTACAAGATG 
CATACTTCTA CTGGAGGAGG CTTCTGTGAC TGTGGAGACA CAGAAGCGTG GAAAACTGGC 
CCTTTTTGTG TGGATCACGA GCCTGGAAGA GCAGGTACTA CAAAAGAGAG CTTACATTGC 
CCATTGAATG AAGAGGTGAT TGCTCAAGCC AGGAGAATAT TCCCTTCGGT GATAAAATAC 
ATTGTAGAAA TGACTATATG GGAAGAAGAA AAGGAATTGC CTCCTGAACT GCAGATAAGG 
GAGAAAAATG AACGATACTA TTGTGTCCTT TTCAACGATG AGCACCATTC GTATGATCAT 
GTGATCTACA GTCTGCAGAG AGCTCTAGAT TGCGAGCTTG CAGAGGCACA GCTGCACACG 
ACTGCCATCG ACAAAGAGGG TCGCCGGGCT GTCAAAGCAG GTGTGTATGC CACTTGCCAG 
GAAGCAAAGG AGGATATAAA GAGTCACTCA GAGAACGTCT CTCAGCACCC CCTCCATGTG 
GAAGTGCTGC ACTCCGTGGT TATGGCTCAC CAGAAATTCG CTCTGCGCCT TGGCTCCTGG 
ATGAACAAAA TTATGAGCTA TTCAAGTGAC TTTAGACAGA TATTTTGCCA GGCCTGCCTC 
GTAGAAGAAC CTGGCTCTGA AAATCCCTGT CTTATAAGCA GACTAATGCT TTGGGATGCA 
AAACTTTATA AAGGTGCCCG TAAGATCCTT CATGAATTGA TCTTTAGTAG TTTTTTTATG 
GAGATGGAAT ACAAAAAACT CTTTGCTATG GAATTTGTGA AGTATTATAA ACAGCTGCAG 
AAAGAGTACA TCAGCGACGA CCACGAGAGA AGCATCTCCA TAACCGCCCT GTCCGTGCAG 
ATGCTCACCG TCCCGACCTT GGCCCGGCAT CTTATTGAAG AGCAGAATGT TATTTCTGTC 
ATTACTGAAA CGCTGCTAGA AGTTTTACCT GAATACTTGG ACAGGAACAA TAAATTCAAC 
TTCCAGGGTT ATAGCCAGGA CAAACTGGGA AGAGTCTACG CAGTTATATG TGACCTAAAG 
TATATCCTGA TTAGCAAGCC TGTCATATGG ACAGAACGAT TAAGAGCGCA GTTCCTGGAA 
GGTTTCCGGT CTTTTCTGAA GATTCTTACC TGTATGCAGG GAATGGAAGA AATCAGAAGA 
CAAGTTGGAC AACACATTGA AGTGGACCCT GACTGGGAGG CTGCCATCGC TATACAGATG 
CAACTAAAGA ATATTTTGCT CATGTTCCAA GAGTGGTGTG CTTGTGATGA AGATCTCTTA 
CTGGTGGCTT ATAAAGAATG TCACAAAGCT GTAATGAGGT GCAGTACAAA TTTCATGTCC 
AGTACCAAGA CAGTAGTGCA ATTGTGCGGT CATAGTCTGG AAACCAAATC CTACAAAGTG 
TCTGAGGACC TTGTAAGCAT ACACCTGCCA CTCTCTAGAA CACTTGCTGG TCTTCATGTA 
CGTTTAAGCA GACTAGGTGC TATTTCAAGA CTGCATGAAT TTGTGCCTTT TGACGGCTTT 
CAAGTAGAGG TCCTGGTGGA GTACCCGCTG CGCTGCCTGG TCCTGGTGGC TCAGGTTGTT 
GCTGAGATGT GGCGAAGAAA CGGGCTCTCA CTCATCAGCC AGGTTTTCTA TTATCAAGAT 
GTTAAATGCA GGGAGGAAAT GTACGATAAA GATATCATCA TGCTTCAGAT TGGAGCATCT 
ATAATGGATC CCAACAAGTT CTTGTTACTG GTACTTCAGA GATATGAACT TACTGATGCT 
TTTAACAAGA CCATATCCAC AAAAGACCAG GATTTGATTA AACAGTATAA TACATTAATA 
GAAGAAATGC TTCAGGTCCT CATCTATATT GTGGGAGAAC GTTATGTACC TGGAGTGGGA 
AATGTTACCA GAGAGGAGGT TATAATGAGA GAGATTACTC ACTTACTTTG CATTGAGCCC 
ATGCCACATA GTGCCATCGC CAGAAACCTA CCTGAGAACG AAAATAATGA AACTGGCTTA 
GAGAATGTCA TAAACAAAGT GGCCACATTT AAGAAACCAG GTGTGTCGGG CCATGGAGTT 
TATGAATTGA AAGATGAATC ACTGAAAGAC TTCAATATGT ACTTTTACCA TTATTCTAAA 
ACACAGCATA GCAAGGCTGA ACATATGCAG AAGAAAAGGA GAAAACAAGA AAATAAAGAT 
GAAGCATTGC CGCCGCCACC TCCTCCGGAG TTCTGCCCTG CTTTCAGCAA AGTAGTCAAC 
CTGCTCAGCT GTGATGTTAT GATGTACATC CTCAGGACCA TCTTTGAGCG GGCAGTGGAC 
ATGGAGTCTA ATCTGTGGAC AGAAGGGATG CTGCAGATGG CGTTCCATAT ATTGGCACTG 
GGCTTGCTGG AAGAGAAGCA GCAGCTTCAG AAAGCTCCTG AAGAGGAAGT GGCTTTTGAC 
TTTTACCATA AGGCTTCAAG ATTGGGAAGT TCAGCCATGA ATGCTCAGAA TATACAAATG 
CTCTTGGAAA AACTCAAAGG AATCCCCCAA TTAGAAAGCC AGAAGGACAT GATAACATGG 
ATACTCCAGA TGTTTGACAC AGTGAAGCGA TTAAGAGAAA AATCTTGTTT AGTTGTGGCA 
ACCACTTCAG GACTGGAGTG CGTTAAGAGT GAGGAGATTA CTCATGATAA AGAAAAGGCA 
GAAAGGAAGA GAAAAGCTGA GGCCGCTAGG CTTCATCGCC AGAAGATCAT GGCCCAGATG 
TCTGCCTTAC AGAAAAACTT CATTGAAACC CACAAACTCA TGTATGATAA TACGTCAGAA 
GTAACAGGGA AGGAAGACTC CATTATGGAG GAAGAGAGCA CCTCAGCAGT CAGTGAGGCC 
TCTAGAATTG CTCTGGGCCC TAAACGGGGC CCGGCTGTTA CTGAAAAGGA GGTGCTGACG 
TGCATCCTCT GCCAAGAAGA ACAAGAGGTG AAACTAGAAA ATAACGCCAT GGTATTGTCA 
GCATGTGTGC AGAAATCCAC CGCCCTAACC CAGCACAGAG GGAAGCCTGT GGACCACTTA 
GGGGAAACAC TGGACCCTCT TTTCATGGAT CCAGACTTGG CACATGGAAC TTATACAGGA 
AGCTGTGGTC ATGTAATGCA TGCAGTGTGC TGGCAGAAGT ATTTTGAAGC TGTGCAGCTG 
AGCTCGCAGC AGCGCATTCA CGTAGACCTG TTTGACCTGG AGAGCGGCGA GTACCTATGC 
CCGCTCTGCA AGTCTCTCTG CAATACTGTC ATCCCCATCA TCCCTTTGCA GCCGCAGAAG 
ATCAACAGTG AGAATGCGGA GGCTCTTGCT CAACTTTTGA CCTTGGCCCG GTGGATACAG 
ACTGTCCTTG CCAGAATATC GGGTTATAAT ATAAAGCATG CTAAAGGAGA AGCCCCAGCA 
GTTCCTGTCT TGTTTAATCA AGGAATGGGG GATTCGACTT TTGAGTTTCA TTCCATCCTG 
AGTTTTGGAG TTCAGTCTTC GGTGAAATAT TCAAATAGTA TCAAGGAAAT GGTCATTCTC 
TTCGCCACAA CAATTTACAG AATTGGCCTG AAAGTGCCTC CTGATGAACT AGACCCACGA 
GTGCCCATGA TGACCTGGAG CACGTGTGCG TTCACCATCC AGGCAATCGA AAACCTGTTG 
GGAGATGAAG GAAAACCTCT ATTTGGAGCA CTTCAAAATA GACAGCATAA CGGTCTGAAG 
GCGCTAATGC AGTTTGCAGT TGCACAGAGG ACTACCTGCC CTCAGGTCCT GATACACAAA 
CATCTGGCTC GGCTCCTGTC AGTTATTCTT CCTAACCTGC AATCAGAAAA TACACCAGGC 
CTTCTGTCCG TGGATCTCTT CCATGTTCTG GTCGGCGCAG TCTTAGCGTT CCCATCCTTG 
TATTGGGATG ACACCGTGGA TCTGCAGCCG TCACCACTTA GTTCTTCATA TAACCACCTC 
TATCTCTTCC ATCTGATCAC CATGGCGCAC ATGCTTCAGA TACTCCTTAC AACAGATACA 
GATCTGTCTT CAGGGCCGCC GCTTGCTGAG GGTGAAGAGG ATAGTGAGGA GGCTCGCTGT 
GCATCTGCTT TCTTTGTGGA AGTGTCGCAG CACACAGACG GCCTCGCTGG GTGCGGTGCT 
CCCGGCTGGT ACCTGTGGCT CTCCCTGAGG AACGGCATCA CCCCTTACCT CCGCTGTGCT 
GCACTGCTTT TCCACTATTT ACTTGGAGTA GCTCCGCCTG AAGAACTGTT TGCCAATTCT 
GCTGAAGGAG AATTCAGTGC ACTCTGTAGC TATCTATCTT TACCCACAAA TTTGTTCCTG 
CTTTTCCAGG AATATTGGGA TACCATAAGG CCCTTACTAC AGAGGTGGTG TGGAGATCCT 
GCCTTACTCA AGTCTTTGAA GCAGAAAAGT GCTGTGGTCA GGTACCCTAG AAAAAGAAAT 
AGTTTGATAG AGCTTCCTGA GGACTACAGC TGTCTTCTAA ATCAGGCTTC TCACTTTAGG 
TGTCCACGGT CTGCAGATGA TGAGCGAAAG CATCCTGTCC TCTGTCTTTT CTGTGGGGCC 
ATCCTGTGTT CTCAGAACAT CTGTTGCCAA GAAATAGTGA ATGGGGAAGA GGTTGGAGCG 
TGCGTTTTTC ATGCGCTTCA TTGTGGTGCT GGAGTCTGCA TTTTCTTAAA AATCCGAGAA 
TGCAGGGTGG TCCTGGTGGA AGGAAAAGCC AGAGGCTGTG CCTACCCAGC CCCTTACTTG 
GATGAATATG GAGAAACAGA CCCAGGGCTA AAGAGAGGAA ACCCACTTCA TTTATCTCGG 
GAGCGGTATC GGAAGCTGCA TTTGGTCTGG CAACAGCACT GCATTATAGA AGAGATTGCT 
CGGAGCCAGG AGACTAATCA GATGCTATTT GGATTCAACT GGCAGTTACT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ubr1 ( XM_006499164.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006499227.1 Mus musculus ubiquitin protein ligase E3 component n-recognin 1 (Ubr1), transcript variant X1, mRNA.

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CloneID	OMu30249
Clone ID Related Accession (Same CDS sequence)	XM_006499164.3 , XM_006499164.2
Accession Version	XM_006499164.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5271bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase UBR1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006499164.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACG AAGAGATGGA CGGCGCCGAG AGGATGGACG TCAGCCCGGA GCCTCCCCTG 
GCCCCGCAGC GGCCGGCATC GTGGTGGGAT CAGCAAGTTG ATTTCTATAC TGCTTTCTTA 
CATCATTTGG CACAATTAGT GCCAGAAATT TATTTTGCTG AGATGGACCC AGATTTGGAA 
AAGCAAGAAG AGAGTGTACA GATGTCAATA CTCACTCCTT TGGAGTGGTA CTTATTTGGA 
GAGGATCCGG ATATTTGCTT AGAGAAATTA AAACACAGTG GAGCGTTCCA GTTGTGTGGG 
AAGGTTTTCA AAAGTGGAGA AACAACATAT TCCTGTAGGG ATTGTGCAAT TGATCCAACG 
TGTGTGCTCT GTATGGACTG CTTCCAAAGT AGTGTTCATA AAAACCATCG TTACAAGATG 
CATACTTCTA CTGGAGGAGG CTTCTGTGAC TGTGGAGACA CAGAAGCGTG GAAAACTGGC 
CCTTTTTGTG TGGATCACGA GCCTGGAAGA GCAGGTACTA CAAAAGAGAG CTTACATTGC 
CCATTGAATG AAGAGGTGAT TGCTCAAGCC AGGAGAATAT TCCCTTCGGT GATAAAATAC 
ATTGTAGAAA TGACTATATG GGAAGAAGAA AAGGAATTGC CTCCTGAACT GCAGATAAGG 
GAGAAAAATG AACGATACTA TTGTGTCCTT TTCAACGATG AGCACCATTC GTATGATCAT 
GTGATCTACA GTCTGCAGAG AGCTCTAGAT TGCGAGCTTG CAGAGGCACA GCTGCACACG 
ACTGCCATCG ACAAAGAGGG TCGCCGGGCT GTCAAAGCAG GTGTGTATGC CACTTGCCAG 
GAAGCAAAGG AGGATATAAA GAGTCACTCA GAGAACGTCT CTCAGCACCC CCTCCATGTG 
GAAGTGCTGC ACTCCGTGGT TATGGCTCAC CAGAAATTCG CTCTGCGCCT TGGCTCCTGG 
ATGAACAAAA TTATGAGCTA TTCAAGTGAC TTTAGACAGA TATTTTGCCA GGCCTGCCTC 
GTAGAAGAAC CTGGCTCTGA AAATCCCTGT CTTATAAGCA GACTAATGCT TTGGGATGCA 
AAACTTTATA AAGGTGCCCG TAAGATCCTT CATGAATTGA TCTTTAGTAG TTTTTTTATG 
GAGATGGAAT ACAAAAAACT CTTTGCTATG GAATTTGTGA AGTATTATAA ACAGCTGCAG 
AAAGAGTACA TCAGCGACGA CCACGAGAGA AGCATCTCCA TAACCGCCCT GTCCGTGCAG 
ATGCTCACCG TCCCGACCTT GGCCCGGCAT CTTATTGAAG AGCAGAATGT TATTTCTGTC 
ATTACTGAAA CGCTGCTAGA AGTTTTACCT GAATACTTGG ACAGGAACAA TAAATTCAAC 
TTCCAGGGTT ATAGCCAGGA CAAACTGGGA AGAGTCTACG CAGTTATATG TGACCTAAAG 
TATATCCTGA TTAGCAAGCC TGTCATATGG ACAGAACGAT TAAGAGCGCA GTTCCTGGAA 
GGTTTCCGGT CTTTTCTGAA GATTCTTACC TGTATGCAGG GAATGGAAGA AATCAGAAGA 
CAAGTTGGAC AACACATTGA AGTGGACCCT GACTGGGAGG CTGCCATCGC TATACAGATG 
CAACTAAAGA ATATTTTGCT CATGTTCCAA GAGTGGTGTG CTTGTGATGA AGATCTCTTA 
CTGGTGGCTT ATAAAGAATG TCACAAAGCT GTAATGAGGT GCAGTACAAA TTTCATGTCC 
AGTACCAAGA CAGTAGTGCA ATTGTGCGGT CATAGTCTGG AAACCAAATC CTACAAAGTG 
TCTGAGGACC TTGTAAGCAT ACACCTGCCA CTCTCTAGAA CACTTGCTGG TCTTCATGTA 
CGTTTAAGCA GACTAGGTGC TATTTCAAGA CTGCATGAAT TTGTGCCTTT TGACGGCTTT 
CAAGTAGAGG TCCTGGTGGA GTACCCGCTG CGCTGCCTGG TCCTGGTGGC TCAGGTTGTT 
GCTGAGATGT GGCGAAGAAA CGGGCTCTCA CTCATCAGCC AGGTTTTCTA TTATCAAGAT 
GTTAAATGCA GGGAGGAAAT GTACGATAAA GATATCATCA TGCTTCAGAT TGGAGCATCT 
ATAATGGATC CCAACAAGTT CTTGTTACTG GTACTTCAGA GATATGAACT TACTGATGCT 
TTTAACAAGA CCATATCCAC AAAAGACCAG GATTTGATTA AACAGTATAA TACATTAATA 
GAAGAAATGC TTCAGGTCCT CATCTATATT GTGGGAGAAC GTTATGTACC TGGAGTGGGA 
AATGTTACCA GAGAGGAGGT TATAATGAGA GAGATTACTC ACTTACTTTG CATTGAGCCC 
ATGCCACATA GTGCCATCGC CAGAAACCTA CCTGAGAACG AAAATAATGA AACTGGCTTA 
GAGAATGTCA TAAACAAAGT GGCCACATTT AAGAAACCAG GTGTGTCGGG CCATGGAGTT 
TATGAATTGA AAGATGAATC ACTGAAAGAC TTCAATATGT ACTTTTACCA TTATTCTAAA 
ACACAGCATA GCAAGGCTGA ACATATGCAG AAGAAAAGGA GAAAACAAGA AAATAAAGAT 
GAAGCATTGC CGCCGCCACC TCCTCCGGAG TTCTGCCCTG CTTTCAGCAA AGTAGTCAAC 
CTGCTCAGCT GTGATGTTAT GATGTACATC CTCAGGACCA TCTTTGAGCG GGCAGTGGAC 
ATGGAGTCTA ATCTGTGGAC AGAAGGGATG CTGCAGATGG CGTTCCATAT ATTGGCACTG 
GGCTTGCTGG AAGAGAAGCA GCAGCTTCAG AAAGCTCCTG AAGAGGAAGT GGCTTTTGAC 
TTTTACCATA AGGCTTCAAG ATTGGGAAGT TCAGCCATGA ATGCTCAGAA TATACAAATG 
CTCTTGGAAA AACTCAAAGG AATCCCCCAA TTAGAAAGCC AGAAGGACAT GATAACATGG 
ATACTCCAGA TGTTTGACAC AGTGAAGCGA TTAAGAGAAA AATCTTGTTT AGTTGTGGCA 
ACCACTTCAG GACTGGAGTG CGTTAAGAGT GAGGAGATTA CTCATGATAA AGAAAAGGCA 
GAAAGGAAGA GAAAAGCTGA GGCCGCTAGG CTTCATCGCC AGAAGATCAT GGCCCAGATG 
TCTGCCTTAC AGAAAAACTT CATTGAAACC CACAAACTCA TGTATGATAA TACGTCAGAA 
GTAACAGGGA AGGAAGACTC CATTATGGAG GAAGAGAGCA CCTCAGCAGT CAGTGAGGCC 
TCTAGAATTG CTCTGGGCCC TAAACGGGGC CCGGCTGTTA CTGAAAAGGA GGTGCTGACG 
TGCATCCTCT GCCAAGAAGA ACAAGAGGTG AAACTAGAAA ATAACGCCAT GGTATTGTCA 
GCATGTGTGC AGAAATCCAC CGCCCTAACC CAGCACAGAG GGAAGCCTGT GGACCACTTA 
GGGGAAACAC TGGACCCTCT TTTCATGGAT CCAGACTTGG CACATGGAAC TTATACAGGA 
AGCTGTGGTC ATGTAATGCA TGCAGTGTGC TGGCAGAAGT ATTTTGAAGC TGTGCAGCTG 
AGCTCGCAGC AGCGCATTCA CGTAGACCTG TTTGACCTGG AGAGCGGCGA GTACCTATGC 
CCGCTCTGCA AGTCTCTCTG CAATACTGTC ATCCCCATCA TCCCTTTGCA GCCGCAGAAG 
ATCAACAGTG AGAATGCGGA GGCTCTTGCT CAACTTTTGA CCTTGGCCCG GTGGATACAG 
ACTGTCCTTG CCAGAATATC GGGTTATAAT ATAAAGCATG CTAAAGAAGC CCCAGCAGTT 
CCTGTCTTGT TTAATCAAGG AATGGGGGAT TCGACTTTTG AGTTTCATTC CATCCTGAGT 
TTTGGAGTTC AGTCTTCGGT GAAATATTCA AATAGTATCA AGGAAATGGT CATTCTCTTC 
GCCACAACAA TTTACAGAAT TGGCCTGAAA GTGCCTCCTG ATGAACTAGA CCCACGAGTG 
CCCATGATGA CCTGGAGCAC GTGTGCGTTC ACCATCCAGG CAATCGAAAA CCTGTTGGGA 
GATGAAGGAA AACCTCTATT TGGAGCACTT CAAAATAGAC AGCATAACGG TCTGAAGGCG 
CTAATGCAGT TTGCAGTTGC ACAGAGGACT ACCTGCCCTC AGGTCCTGAT ACACAAACAT 
CTGGCTCGGC TCCTGTCAGT TATTCTTCCT AACCTGCAAT CAGAAAATAC ACCAGGCCTT 
CTGTCCGTGG ATCTCTTCCA TGTTCTGGTC GGCGCAGTCT TAGCGTTCCC ATCCTTGTAT 
TGGGATGACA CCGTGGATCT GCAGCCGTCA CCACTTAGTT CTTCATATAA CCACCTCTAT 
CTCTTCCATC TGATCACCAT GGCGCACATG CTTCAGATAC TCCTTACAAC AGATACAGAT 
CTGTCTTCAG GGCCGCCGCT TGCTGAGGGT GAAGAGGATA GTGAGGAGGC TCGCTGTGCA 
TCTGCTTTCT TTGTGGAAGT GTCGCAGCAC ACAGACGGCC TCGCTGGGTG CGGTGCTCCC 
GGCTGGTACC TGTGGCTCTC CCTGAGGAAC GGCATCACCC CTTACCTCCG CTGTGCTGCA 
CTGCTTTTCC ACTATTTACT TGGAGTAGCT CCGCCTGAAG AACTGTTTGC CAATTCTGCT 
GAAGGAGAAT TCAGTGCACT CTGTAGCTAT CTATCTTTAC CCACAAATTT GTTCCTGCTT 
TTCCAGGAAT ATTGGGATAC CATAAGGCCC TTACTACAGA GGTGGTGTGG AGATCCTGCC 
TTACTCAAGT CTTTGAAGCA GAAAAGTGCT GTGGTCAGGT ACCCTAGAAA AAGAAATAGT 
TTGATAGAGC TTCCTGAGGA CTACAGCTGT CTTCTAAATC AGGCTTCTCA CTTTAGGTGT 
CCACGGTCTG CAGATGATGA GCGAAAGCAT CCTGTCCTCT GTCTTTTCTG TGGGGCCATC 
CTGTGTTCTC AGAACATCTG TTGCCAAGAA ATAGTGAATG GGGAAGAGGT TGGAGCGTGC 
GTTTTTCATG CGCTTCATTG TGGTGCTGGA GTCTGCATTT TCTTAAAAAT CCGAGAATGC 
AGGGTGGTCC TGGTGGAAGG AAAAGCCAGA GGCTGTGCCT ACCCAGCCCC TTACTTGGAT 
GAATATGGAG AAACAGACCC AGGGCTAAAG AGAGGAAACC CACTTCATTT ATCTCGGGAG 
CGGTATCGGA AGCTGCATTT GGTCTGGCAA CAGCACTGCA TTATAGAAGA GATTGCTCGG 
AGCCAGGAGA CTAATCAGAT GCTATTTGGA TTCAACTGGC AGTTACTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006499227.1
CDS	109..5379
Translation	MADEEMDGAERMDVSPEPPLAPQRPASWWDQQVDFYTAFLHHLAQLVPEIYFAEMDPDLEKQEESVQMSILTPLEWYLFGEDPDICLEKLKHSGAFQLCGKVFKSGETTYSCRDCAIDPTCVLCMDCFQSSVHKNHRYKMHTSTGGGFCDCGDTEAWKTGPFCVDHEPGRAGTTKESLHCPLNEEVIAQARRIFPSVIKYIVEMTIWEEEKELPPELQIREKNERYYCVLFNDEHHSYDHVIYSLQRALDCELAEAQLHTTAIDKEGRRAVKAGVYATCQEAKEDIKSHSENVSQHPLHVEVLHSVVMAHQKFALRLGSWMNKIMSYSSDFRQIFCQACLVEEPGSENPCLISRLMLWDAKLYKGARKILHELIFSSFFMEMEYKKLFAMEFVKYYKQLQKEYISDDHERSISITALSVQMLTVPTLARHLIEEQNVISVITETLLEVLPEYLDRNNKFNFQGYSQDKLGRVYAVICDLKYILISKPVIWTERLRAQFLEGFRSFLKILTCMQGMEEIRRQVGQHIEVDPDWEAAIAIQMQLKNILLMFQEWCACDEDLLLVAYKECHKAVMRCSTNFMSSTKTVVQLCGHSLETKSYKVSEDLVSIHLPLSRTLAGLHVRLSRLGAISRLHEFVPFDGFQVEVLVEYPLRCLVLVAQVVAEMWRRNGLSLISQVFYYQDVKCREEMYDKDIIMLQIGASIMDPNKFLLLVLQRYELTDAFNKTISTKDQDLIKQYNTLIEEMLQVLIYIVGERYVPGVGNVTREEVIMREITHLLCIEPMPHSAIARNLPENENNETGLENVINKVATFKKPGVSGHGVYELKDESLKDFNMYFYHYSKTQHSKAEHMQKKRRKQENKDEALPPPPPPEFCPAFSKVVNLLSCDVMMYILRTIFERAVDMESNLWTEGMLQMAFHILALGLLEEKQQLQKAPEEEVAFDFYHKASRLGSSAMNAQNIQMLLEKLKGIPQLESQKDMITWILQMFDTVKRLREKSCLVVATTSGLECVKSEEITHDKEKAERKRKAEAARLHRQKIMAQMSALQKNFIETHKLMYDNTSEVTGKEDSIMEEESTSAVSEASRIALGPKRGPAVTEKEVLTCILCQEEQEVKLENNAMVLSACVQKSTALTQHRGKPVDHLGETLDPLFMDPDLAHGTYTGSCGHVMHAVCWQKYFEAVQLSSQQRIHVDLFDLESGEYLCPLCKSLCNTVIPIIPLQPQKINSENAEALAQLLTLARWIQTVLARISGYNIKHAKEAPAVPVLFNQGMGDSTFEFHSILSFGVQSSVKYSNSIKEMVILFATTIYRIGLKVPPDELDPRVPMMTWSTCAFTIQAIENLLGDEGKPLFGALQNRQHNGLKALMQFAVAQRTTCPQVLIHKHLARLLSVILPNLQSENTPGLLSVDLFHVLVGAVLAFPSLYWDDTVDLQPSPLSSSYNHLYLFHLITMAHMLQILLTTDTDLSSGPPLAEGEEDSEEARCASAFFVEVSQHTDGLAGCGAPGWYLWLSLRNGITPYLRCAALLFHYLLGVAPPEELFANSAEGEFSALCSYLSLPTNLFLLFQEYWDTIRPLLQRWCGDPALLKSLKQKSAVVRYPRKRNSLIELPEDYSCLLNQASHFRCPRSADDERKHPVLCLFCGAILCSQNICCQEIVNGEEVGACVFHALHCGAGVCIFLKIRECRVVLVEGKARGCAYPAPYLDEYGETDPGLKRGNPLHLSRERYRKLHLVWQQHCIIEEIARSQETNQMLFGFNWQLL

Target ORF information:

RefSeq Version	XM_006499164.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin protein ligase E3 component n-recognin 1 (Ubr1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006499164.2

ORF Insert Sequence:

ATGGCGGACG AAGAGATGGA CGGCGCCGAG AGGATGGACG TCAGCCCGGA GCCTCCCCTG 
GCCCCGCAGC GGCCGGCATC GTGGTGGGAT CAGCAAGTTG ATTTCTATAC TGCTTTCTTA 
CATCATTTGG CACAATTAGT GCCAGAAATT TATTTTGCTG AGATGGACCC AGATTTGGAA 
AAGCAAGAAG AGAGTGTACA GATGTCAATA CTCACTCCTT TGGAGTGGTA CTTATTTGGA 
GAGGATCCGG ATATTTGCTT AGAGAAATTA AAACACAGTG GAGCGTTCCA GTTGTGTGGG 
AAGGTTTTCA AAAGTGGAGA AACAACATAT TCCTGTAGGG ATTGTGCAAT TGATCCAACG 
TGTGTGCTCT GTATGGACTG CTTCCAAAGT AGTGTTCATA AAAACCATCG TTACAAGATG 
CATACTTCTA CTGGAGGAGG CTTCTGTGAC TGTGGAGACA CAGAAGCGTG GAAAACTGGC 
CCTTTTTGTG TGGATCACGA GCCTGGAAGA GCAGGTACTA CAAAAGAGAG CTTACATTGC 
CCATTGAATG AAGAGGTGAT TGCTCAAGCC AGGAGAATAT TCCCTTCGGT GATAAAATAC 
ATTGTAGAAA TGACTATATG GGAAGAAGAA AAGGAATTGC CTCCTGAACT GCAGATAAGG 
GAGAAAAATG AACGATACTA TTGTGTCCTT TTCAACGATG AGCACCATTC GTATGATCAT 
GTGATCTACA GTCTGCAGAG AGCTCTAGAT TGCGAGCTTG CAGAGGCACA GCTGCACACG 
ACTGCCATCG ACAAAGAGGG TCGCCGGGCT GTCAAAGCAG GTGTGTATGC CACTTGCCAG 
GAAGCAAAGG AGGATATAAA GAGTCACTCA GAGAACGTCT CTCAGCACCC CCTCCATGTG 
GAAGTGCTGC ACTCCGTGGT TATGGCTCAC CAGAAATTCG CTCTGCGCCT TGGCTCCTGG 
ATGAACAAAA TTATGAGCTA TTCAAGTGAC TTTAGACAGA TATTTTGCCA GGCCTGCCTC 
GTAGAAGAAC CTGGCTCTGA AAATCCCTGT CTTATAAGCA GACTAATGCT TTGGGATGCA 
AAACTTTATA AAGGTGCCCG TAAGATCCTT CATGAATTGA TCTTTAGTAG TTTTTTTATG 
GAGATGGAAT ACAAAAAACT CTTTGCTATG GAATTTGTGA AGTATTATAA ACAGCTGCAG 
AAAGAGTACA TCAGCGACGA CCACGAGAGA AGCATCTCCA TAACCGCCCT GTCCGTGCAG 
ATGCTCACCG TCCCGACCTT GGCCCGGCAT CTTATTGAAG AGCAGAATGT TATTTCTGTC 
ATTACTGAAA CGCTGCTAGA AGTTTTACCT GAATACTTGG ACAGGAACAA TAAATTCAAC 
TTCCAGGGTT ATAGCCAGGA CAAACTGGGA AGAGTCTACG CAGTTATATG TGACCTAAAG 
TATATCCTGA TTAGCAAGCC TGTCATATGG ACAGAACGAT TAAGAGCGCA GTTCCTGGAA 
GGTTTCCGGT CTTTTCTGAA GATTCTTACC TGTATGCAGG GAATGGAAGA AATCAGAAGA 
CAAGTTGGAC AACACATTGA AGTGGACCCT GACTGGGAGG CTGCCATCGC TATACAGATG 
CAACTAAAGA ATATTTTGCT CATGTTCCAA GAGTGGTGTG CTTGTGATGA AGATCTCTTA 
CTGGTGGCTT ATAAAGAATG TCACAAAGCT GTAATGAGGT GCAGTACAAA TTTCATGTCC 
AGTACCAAGA CAGTAGTGCA ATTGTGCGGT CATAGTCTGG AAACCAAATC CTACAAAGTG 
TCTGAGGACC TTGTAAGCAT ACACCTGCCA CTCTCTAGAA CACTTGCTGG TCTTCATGTA 
CGTTTAAGCA GACTAGGTGC TATTTCAAGA CTGCATGAAT TTGTGCCTTT TGACGGCTTT 
CAAGTAGAGG TCCTGGTGGA GTACCCGCTG CGCTGCCTGG TCCTGGTGGC TCAGGTTGTT 
GCTGAGATGT GGCGAAGAAA CGGGCTCTCA CTCATCAGCC AGGTTTTCTA TTATCAAGAT 
GTTAAATGCA GGGAGGAAAT GTACGATAAA GATATCATCA TGCTTCAGAT TGGAGCATCT 
ATAATGGATC CCAACAAGTT CTTGTTACTG GTACTTCAGA GATATGAACT TACTGATGCT 
TTTAACAAGA CCATATCCAC AAAAGACCAG GATTTGATTA AACAGTATAA TACATTAATA 
GAAGAAATGC TTCAGGTCCT CATCTATATT GTGGGAGAAC GTTATGTACC TGGAGTGGGA 
AATGTTACCA GAGAGGAGGT TATAATGAGA GAGATTACTC ACTTACTTTG CATTGAGCCC 
ATGCCACATA GTGCCATCGC CAGAAACCTA CCTGAGAACG AAAATAATGA AACTGGCTTA 
GAGAATGTCA TAAACAAAGT GGCCACATTT AAGAAACCAG GTGTGTCGGG CCATGGAGTT 
TATGAATTGA AAGATGAATC ACTGAAAGAC TTCAATATGT ACTTTTACCA TTATTCTAAA 
ACACAGCATA GCAAGGCTGA ACATATGCAG AAGAAAAGGA GAAAACAAGA AAATAAAGAT 
GAAGCATTGC CGCCGCCACC TCCTCCGGAG TTCTGCCCTG CTTTCAGCAA AGTAGTCAAC 
CTGCTCAGCT GTGATGTTAT GATGTACATC CTCAGGACCA TCTTTGAGCG GGCAGTGGAC 
ATGGAGTCTA ATCTGTGGAC AGAAGGGATG CTGCAGATGG CGTTCCATAT ATTGGCACTG 
GGCTTGCTGG AAGAGAAGCA GCAGCTTCAG AAAGCTCCTG AAGAGGAAGT GGCTTTTGAC 
TTTTACCATA AGGCTTCAAG ATTGGGAAGT TCAGCCATGA ATGCTCAGAA TATACAAATG 
CTCTTGGAAA AACTCAAAGG AATCCCCCAA TTAGAAAGCC AGAAGGACAT GATAACATGG 
ATACTCCAGA TGTTTGACAC AGTGAAGCGA TTAAGAGAAA AATCTTGTTT AGTTGTGGCA 
ACCACTTCAG GACTGGAGTG CGTTAAGAGT GAGGAGATTA CTCATGATAA AGAAAAGGCA 
GAAAGGAAGA GAAAAGCTGA GGCCGCTAGG CTTCATCGCC AGAAGATCAT GGCCCAGATG 
TCTGCCTTAC AGAAAAACTT CATTGAAACC CACAAACTCA TGTATGATAA TACGTCAGAA 
GTAACAGGGA AGGAAGACTC CATTATGGAG GAAGAGAGCA CCTCAGCAGT CAGTGAGGCC 
TCTAGAATTG CTCTGGGCCC TAAACGGGGC CCGGCTGTTA CTGAAAAGGA GGTGCTGACG 
TGCATCCTCT GCCAAGAAGA ACAAGAGGTG AAACTAGAAA ATAACGCCAT GGTATTGTCA 
GCATGTGTGC AGAAATCCAC CGCCCTAACC CAGCACAGAG GGAAGCCTGT GGACCACTTA 
GGGGAAACAC TGGACCCTCT TTTCATGGAT CCAGACTTGG CACATGGAAC TTATACAGGA 
AGCTGTGGTC ATGTAATGCA TGCAGTGTGC TGGCAGAAGT ATTTTGAAGC TGTGCAGCTG 
AGCTCGCAGC AGCGCATTCA CGTAGACCTG TTTGACCTGG AGAGCGGCGA GTACCTATGC 
CCGCTCTGCA AGTCTCTCTG CAATACTGTC ATCCCCATCA TCCCTTTGCA GCCGCAGAAG 
ATCAACAGTG AGAATGCGGA GGCTCTTGCT CAACTTTTGA CCTTGGCCCG GTGGATACAG 
ACTGTCCTTG CCAGAATATC GGGTTATAAT ATAAAGCATG CTAAAGAAGC CCCAGCAGTT 
CCTGTCTTGT TTAATCAAGG AATGGGGGAT TCGACTTTTG AGTTTCATTC CATCCTGAGT 
TTTGGAGTTC AGTCTTCGGT GAAATATTCA AATAGTATCA AGGAAATGGT CATTCTCTTC 
GCCACAACAA TTTACAGAAT TGGCCTGAAA GTGCCTCCTG ATGAACTAGA CCCACGAGTG 
CCCATGATGA CCTGGAGCAC GTGTGCGTTC ACCATCCAGG CAATCGAAAA CCTGTTGGGA 
GATGAAGGAA AACCTCTATT TGGAGCACTT CAAAATAGAC AGCATAACGG TCTGAAGGCG 
CTAATGCAGT TTGCAGTTGC ACAGAGGACT ACCTGCCCTC AGGTCCTGAT ACACAAACAT 
CTGGCTCGGC TCCTGTCAGT TATTCTTCCT AACCTGCAAT CAGAAAATAC ACCAGGCCTT 
CTGTCCGTGG ATCTCTTCCA TGTTCTGGTC GGCGCAGTCT TAGCGTTCCC ATCCTTGTAT 
TGGGATGACA CCGTGGATCT GCAGCCGTCA CCACTTAGTT CTTCATATAA CCACCTCTAT 
CTCTTCCATC TGATCACCAT GGCGCACATG CTTCAGATAC TCCTTACAAC AGATACAGAT 
CTGTCTTCAG GGCCGCCGCT TGCTGAGGGT GAAGAGGATA GTGAGGAGGC TCGCTGTGCA 
TCTGCTTTCT TTGTGGAAGT GTCGCAGCAC ACAGACGGCC TCGCTGGGTG CGGTGCTCCC 
GGCTGGTACC TGTGGCTCTC CCTGAGGAAC GGCATCACCC CTTACCTCCG CTGTGCTGCA 
CTGCTTTTCC ACTATTTACT TGGAGTAGCT CCGCCTGAAG AACTGTTTGC CAATTCTGCT 
GAAGGAGAAT TCAGTGCACT CTGTAGCTAT CTATCTTTAC CCACAAATTT GTTCCTGCTT 
TTCCAGGAAT ATTGGGATAC CATAAGGCCC TTACTACAGA GGTGGTGTGG AGATCCTGCC 
TTACTCAAGT CTTTGAAGCA GAAAAGTGCT GTGGTCAGGT ACCCTAGAAA AAGAAATAGT 
TTGATAGAGC TTCCTGAGGA CTACAGCTGT CTTCTAAATC AGGCTTCTCA CTTTAGGTGT 
CCACGGTCTG CAGATGATGA GCGAAAGCAT CCTGTCCTCT GTCTTTTCTG TGGGGCCATC 
CTGTGTTCTC AGAACATCTG TTGCCAAGAA ATAGTGAATG GGGAAGAGGT TGGAGCGTGC 
GTTTTTCATG CGCTTCATTG TGGTGCTGGA GTCTGCATTT TCTTAAAAAT CCGAGAATGC 
AGGGTGGTCC TGGTGGAAGG AAAAGCCAGA GGCTGTGCCT ACCCAGCCCC TTACTTGGAT 
GAATATGGAG AAACAGACCC AGGGCTAAAG AGAGGAAACC CACTTCATTT ATCTCGGGAG 
CGGTATCGGA AGCTGCATTT GGTCTGGCAA CAGCACTGCA TTATAGAAGA GATTGCTCGG 
AGCCAGGAGA CTAATCAGAT GCTATTTGGA TTCAACTGGC AGTTACTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The N-end rule pathway counteracts cell death by destroying proapoptotic protein fragments.
Proceedings of the National Academy of Sciences of the United States of America109(27)E1839-47(2012 Jul)
Piatkov KI,Brower CS,Varshavsky A

The substrate recognition domains of the N-end rule pathway.
The Journal of biological chemistry284(3)1884-95(2009 Jan)
Tasaki T,Zakrzewska A,Dudgeon DD,Jiang Y,Lazo JS,Kwon YT

Roles of STAT3/SOCS3 pathway in regulating the visual function and ubiquitin-proteasome-dependent degradation of rhodopsin during retinal inflammation.
The Journal of biological chemistry283(36)24561-70(2008 Sep)
Ozawa Y,Nakao K,Kurihara T,Shimazaki T,Shimmura S,Ishida S,Yoshimura A,Tsubota K,Okano H

Synthetic heterovalent inhibitors targeting recognition E3 components of the N-end rule pathway.
Proceedings of the National Academy of Sciences of the United States of America105(1)100-5(2008 Jan)
Lee MJ,Pal K,Tasaki T,Roy S,Jiang Y,An JY,Banerjee R,Kwon YT

Biochemical and genetic studies of UBR3, a ubiquitin ligase with a function in olfactory and other sensory systems.
The Journal of biological chemistry282(25)18510-20(2007 Jun)
Tasaki T,Sohr R,Xia Z,Hellweg R,H?rtnagl H,Varshavsky A,Kwon YT

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Ubr1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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