Ube2m cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ube2m
Entrez Gene ID	22192
Full Name	ubiquitin-conjugating enzyme E2M
Synonyms	2510040H03Rik,UBC12,Ubc-rs2
General protein information	Preferred Names ubiquitin-conjugating enzyme E2M Names NEDD8-conjugating enzyme Ubc12 NEDD8 carrier protein NEDD8 protein ligase UBC12 homolog, yeast ubiquitin C, related sequence 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7 A1\|7 7.73 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_145578.3	NP_663553.1	NEDD8-conjugating enzyme Ubc12 isoform 1
NM_001243968.1	NP_001230897.1	NEDD8-conjugating enzyme Ubc12 isoform 3
NM_001168469.2	NP_001161941.1	NEDD8-conjugating enzyme Ubc12 isoform 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	UBE2M	XP_001146573.2
	UBE2M	XP_001099932.1
Bos taurus (cattle)	UBE2M	NP_001076972.1
Schizosaccharomyces pombe (fission yeast)	ubc12	NP_588256.1
Mus musculus (house mouse)	Ube2m	NP_663553.1
Magnaporthe oryzae (rice blast fungus)	MGG_01807	XP_003714818.1
Neurospora crassa	NCU03610	XP_961337.1
Oryza sativa (rice)	Os09g0321900	NP_001062859.1
Homo sapiens (human)	UBE2M	NP_003960.1
Canis lupus familiaris (dog)	UBE2M	XP_541337.3
Drosophila melanogaster (fruit fly)	Ubc12	NP_001261568.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005916	XP_315947.2
Saccharomyces cerevisiae (baker's yeast)	UBC12	NP_013409.1
Arabidopsis thaliana (thale cress)	RCE1	NP_001154289.1
Arabidopsis thaliana (thale cress)	AT2G18600	NP_565440.1
Rattus norvegicus (Norway rat)	Ube2m	NP_001101941.1
Danio rerio (zebrafish)	wu:fb58e05	XP_001920670.3
Kluyveromyces lactis	KLLA0C17248g	XP_452971.1
Oryza sativa (rice)	Os08g0374100	NP_001061664.1
Eremothecium gossypii	AGOS_ADL035C	NP_984062.1
Xenopus tropicalis (tropical clawed frog)	ube2m	NP_988956.1

Gene Ontology
Bibliography

Related articles in PubMed

Systematic screening for skin, hair, and nail abnormalities in a large-scale knockout mouse program.
Sundberg JP, Dadras SS, Silva KA, Kennedy VE, Garland G, Murray SA, Sundberg BA, Schofield PN, Pratt CH
PloS one12(7)e0180682(2017)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Enteric pathogens deploy cell cycle inhibiting factors to block the bactericidal activity of Perforin-2.
McCormack RM, Lyapichev K, Olsson ML, Podack ER, Munson GP
eLife4(2015 Sep)

Neddylation inhibition impairs spine development, destabilizes synapses and deteriorates cognition.
Vogl AM, Brockmann MM, Giusti SA, Maccarrone G, Vercelli CA, Bauder CA, Richter JS, Roselli F, Hafner AS, Dedic N, Wotjak CT, Vogt-Weisenhorn DM, Choquet D, Turck CW, Stein V, Deussing JM, Refojo D
Nature neuroscience18(2)239-51(2015 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SCCRO recruits Ubc12 approximately NEDD8 to the CAND1-Cul1-ROC1 complex but that this is not sufficient to dissociate or overcome the inhibitory effects of CAND1 on cullin neddylation
Title: SCCRO (DCUN1D1) is an essential component of the E3 complex for neddylation.

The following Ube2m gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ube2m cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu11415	NM_001168469.2 Latest version!	Mus musculus ubiquitin-conjugating enzyme E2M (Ube2m), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OMu04395	NM_001243968.1 Latest version!	Mus musculus ubiquitin-conjugating enzyme E2M (Ube2m), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu11817	NM_145578.3 Latest version!	Mus musculus ubiquitin-conjugating enzyme E2M (Ube2m), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ube2m ( NM_001168469.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001161941.1 Mus musculus ubiquitin-conjugating enzyme E2M (Ube2m), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu11415
Clone ID Related Accession (Same CDS sequence)	NM_001168469.2
Accession Version	NM_001168469.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Mus musculus(house mouse)
Product	NEDD8-conjugating enzyme Ubc12 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC121301.8. On Sep 6, 2011 this sequence version replaced NM_001168469.1. Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at a downstream, in-frame start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BE569757.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849388, SAMN01164131 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTTTATC ATCCCAACAT TGACCTCGAG GGCAACGTCT GCCTCAACAT CCTCAGAGAG 
GACTGGAAGC CAGTCCTTAC GATAAACTCC ATAATTTATG GCCTGCAGTA TCTCTTCTTG 
GAGCCGAACC CTGAGGACCC ACTGAACAAG GAGGCTGCTG AGGTCCTGCA GAACAACCGG 
CGGCTGTTTG AACAAAATGT GCAGCGCTCC ATGAGAGGTG GTTACATCGG GTCCACCTAC 
TTCGAGCGCT GCCTGAAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001161941.1
CDS	474..734
Translation	MVYHPNIDLEGNVCLNILREDWKPVLTINSIIYGLQYLFLEPNPEDPLNKEAAEVLQNNRRLFEQNVQRSMRGGYIGSTYFERCLK

Target ORF information:

RefSeq Version	NM_001168469.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin-conjugating enzyme E2M (Ube2m), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001168469.2

ORF Insert Sequence:

ATGGTTTATC ATCCCAACAT TGACCTCGAG GGCAACGTCT GCCTCAACAT CCTCAGAGAG 
GACTGGAAGC CAGTCCTTAC GATAAACTCC ATAATTTATG GCCTGCAGTA TCTCTTCTTG 
GAGCCGAACC CTGAGGACCC ACTGAACAAG GAGGCTGCTG AGGTCCTGCA GAACAACCGG 
CGGCTGTTTG AACAAAATGT GCAGCGCTCC ATGAGAGGTG GTTACATCGG GTCCACCTAC 
TTCGAGCGCT GCCTGAAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ube2m ( NM_001243968.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001230897.1 Mus musculus ubiquitin-conjugating enzyme E2M (Ube2m), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu04395
Clone ID Related Accession (Same CDS sequence)	NM_001243968.1
Accession Version	NM_001243968.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Mus musculus(house mouse)
Product	NEDD8-conjugating enzyme Ubc12 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC121301.8. Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (3) is shorter and has a distinct N-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BE624961.1, BI412129.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGGCCCC TGATATTTGA CATTAACGAG CTGAACCTGC CCAAGACGTG TGACATCAGC 
TTCTCAGACC CAGACGACCT CCTCAACTTC AAGCTGGTGA TCTGTCCTGA TGAAGGCTTC 
TACAAAAGTG GCAAGTTTGT ATTCAGCTTT AAGGTGGGAC AGGGTTACCC ACATGACCCT 
CCCAAGGTGA AGTGTGAAAC AATGGTTTAT CATCCCAACA TTGACCTCGA GGGCAACGTC 
TGCCTCAACA TCCTCAGAGA GGACTGGAAG CCAGTCCTTA CGATAAACTC CATAATTTAT 
GGCCTGCAGT ATCTCTTCTT GGAGCCGAAC CCTGAGGACC CACTGAACAA GGAGGCTGCT 
GAGGTCCTGC AGAACAACCG GCGGCTGTTT GAACAAAATG TGCAGCGCTC CATGAGAGGT 
GGTTACATCG GGTCCACCTA CTTCGAGCGC TGCCTGAAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001230897.1
CDS	55..516
Translation	MRPLIFDINELNLPKTCDISFSDPDDLLNFKLVICPDEGFYKSGKFVFSFKVGQGYPHDPPKVKCETMVYHPNIDLEGNVCLNILREDWKPVLTINSIIYGLQYLFLEPNPEDPLNKEAAEVLQNNRRLFEQNVQRSMRGGYIGSTYFERCLK

Target ORF information:

RefSeq Version	NM_001243968.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin-conjugating enzyme E2M (Ube2m), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001243968.1

ORF Insert Sequence:

ATGCGGCCCC TGATATTTGA CATTAACGAG CTGAACCTGC CCAAGACGTG TGACATCAGC 
TTCTCAGACC CAGACGACCT CCTCAACTTC AAGCTGGTGA TCTGTCCTGA TGAAGGCTTC 
TACAAAAGTG GCAAGTTTGT ATTCAGCTTT AAGGTGGGAC AGGGTTACCC ACATGACCCT 
CCCAAGGTGA AGTGTGAAAC AATGGTTTAT CATCCCAACA TTGACCTCGA GGGCAACGTC 
TGCCTCAACA TCCTCAGAGA GGACTGGAAG CCAGTCCTTA CGATAAACTC CATAATTTAT 
GGCCTGCAGT ATCTCTTCTT GGAGCCGAAC CCTGAGGACC CACTGAACAA GGAGGCTGCT 
GAGGTCCTGC AGAACAACCG GCGGCTGTTT GAACAAAATG TGCAGCGCTC CATGAGAGGT 
GGTTACATCG GGTCCACCTA CTTCGAGCGC TGCCTGAAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ube2m ( NM_145578.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_663553.1 Mus musculus ubiquitin-conjugating enzyme E2M (Ube2m), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu11817
Clone ID Related Accession (Same CDS sequence)	NM_145578.3
Accession Version	NM_145578.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Mus musculus(house mouse)
Product	NEDD8-conjugating enzyme Ubc12 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC121301.8. On Sep 6, 2011 this sequence version replaced NM_145578.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC021792.1, BU514112.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849388, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATCAAGC TGTTCTCGCT GAAGCAGCAG AAGAAGGAGG AGGAGTCGGC CGGCGGCACC 
AAGGGCAGCA GCAAGAAGGC GTCGGCGGCG CAGCTCCGGA TTCAGAAGGA CATTAACGAG 
CTGAACCTGC CCAAGACGTG TGACATCAGC TTCTCAGACC CAGACGACCT CCTCAACTTC 
AAGCTGGTGA TCTGTCCTGA TGAAGGCTTC TACAAAAGTG GCAAGTTTGT ATTCAGCTTT 
AAGGTGGGAC AGGGTTACCC ACATGACCCT CCCAAGGTGA AGTGTGAAAC AATGGTTTAT 
CATCCCAACA TTGACCTCGA GGGCAACGTC TGCCTCAACA TCCTCAGAGA GGACTGGAAG 
CCAGTCCTTA CGATAAACTC CATAATTTAT GGCCTGCAGT ATCTCTTCTT GGAGCCGAAC 
CCTGAGGACC CACTGAACAA GGAGGCTGCT GAGGTCCTGC AGAACAACCG GCGGCTGTTT 
GAACAAAATG TGCAGCGCTC CATGAGAGGT GGTTACATCG GGTCCACCTA CTTCGAGCGC 
TGCCTGAAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_663553.1
CDS	250..801
Translation	MIKLFSLKQQKKEEESAGGTKGSSKKASAAQLRIQKDINELNLPKTCDISFSDPDDLLNFKLVICPDEGFYKSGKFVFSFKVGQGYPHDPPKVKCETMVYHPNIDLEGNVCLNILREDWKPVLTINSIIYGLQYLFLEPNPEDPLNKEAAEVLQNNRRLFEQNVQRSMRGGYIGSTYFERCLK

Target ORF information:

RefSeq Version	NM_145578.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin-conjugating enzyme E2M (Ube2m), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145578.3

ORF Insert Sequence:

ATGATCAAGC TGTTCTCGCT GAAGCAGCAG AAGAAGGAGG AGGAGTCGGC CGGCGGCACC 
AAGGGCAGCA GCAAGAAGGC GTCGGCGGCG CAGCTCCGGA TTCAGAAGGA CATTAACGAG 
CTGAACCTGC CCAAGACGTG TGACATCAGC TTCTCAGACC CAGACGACCT CCTCAACTTC 
AAGCTGGTGA TCTGTCCTGA TGAAGGCTTC TACAAAAGTG GCAAGTTTGT ATTCAGCTTT 
AAGGTGGGAC AGGGTTACCC ACATGACCCT CCCAAGGTGA AGTGTGAAAC AATGGTTTAT 
CATCCCAACA TTGACCTCGA GGGCAACGTC TGCCTCAACA TCCTCAGAGA GGACTGGAAG 
CCAGTCCTTA CGATAAACTC CATAATTTAT GGCCTGCAGT ATCTCTTCTT GGAGCCGAAC 
CCTGAGGACC CACTGAACAA GGAGGCTGCT GAGGTCCTGC AGAACAACCG GCGGCTGTTT 
GAACAAAATG TGCAGCGCTC CATGAGAGGT GGTTACATCG GGTCCACCTA CTTCGAGCGC 
TGCCTGAAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Systematic screening for skin, hair, and nail abnormalities in a large-scale knockout mouse program.
PloS one12(7)e0180682(2017)
Sundberg JP,Dadras SS,Silva KA,Kennedy VE,Garland G,Murray SA,Sundberg BA,Schofield PN,Pratt CH

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Enteric pathogens deploy cell cycle inhibiting factors to block the bactericidal activity of Perforin-2.
eLife4(2015 Sep)
McCormack RM,Lyapichev K,Olsson ML,Podack ER,Munson GP

Neddylation inhibition impairs spine development, destabilizes synapses and deteriorates cognition.
Nature neuroscience18(2)239-51(2015 Feb)
Vogl AM,Brockmann MM,Giusti SA,Maccarrone G,Vercelli CA,Bauder CA,Richter JS,Roselli F,Hafner AS,Dedic N,Wotjak CT,Vogt-Weisenhorn DM,Choquet D,Turck CW,Stein V,Deussing JM,Refojo D

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Ube2m cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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