Tyms cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tyms
Entrez Gene ID	22171
Full Name	thymidylate synthase
Synonyms	Ts
General protein information	Preferred Names thymidylate synthase Names thymidylate synthase Tsase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5 B1\|5 15.81 cM
Summary	This gene encodes an enzyme that catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate as a cofactor. This function maintains the thymidine-5-prime monophosphate concentration critical for DNA replication and repair. The encoded enzyme is a target for cancer chemotherapeutic agents. The majority of transcripts for this gene lack a 3' UTR (PMID: 3022294, 3444407). The stop codon in these transcripts is UAA, compared to the UAG found in the genome and longer transcripts, as the polyA site is located within the stop codon (PMID: 3444407, 2157203). A related pseudogene has been identified on chromosome 10. [provided by RefSeq, Mar 2010].

mRNA and Protein(s)

mRNA	Protein	Name
NM_021288.4	NP_067263.1	thymidylate synthase
XM_011249762.2	XP_011248064.1	thymidylate synthase isoform X1
XM_017320801.1	XP_017176290.1	thymidylate synthase isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	TYMS	NP_001062.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010457	XP_311491.3
Arabidopsis thaliana (thale cress)	AT2G21550	NP_179750.1
Canis lupus familiaris (dog)	TYMS	NP_001239103.1
Saccharomyces cerevisiae (baker's yeast)	CDC21	NP_014717.2
Eremothecium gossypii	AGOS_ADR219C	NP_984315.1
Magnaporthe oryzae (rice blast fungus)	MGG_04458	XP_003713554.1
Arabidopsis thaliana (thale cress)	THY-2	NP_195183.2
Xenopus tropicalis (tropical clawed frog)	tyms	NP_001072852.1
Rattus norvegicus (Norway rat)	Tyms	NP_062052.1
Gallus gallus (chicken)	TYMS	XP_419147.3
Drosophila melanogaster (fruit fly)	Ts	NP_477367.1
Schizosaccharomyces pombe (fission yeast)	SPAC15E1.04	NP_594305.1
Mus musculus (house mouse)	Tyms	NP_067263.1
Caenorhabditis elegans (roundworm)	Y110A7A.4	NP_491532.3
Neurospora crassa	NCU10053	XP_957941.1
Pan troglodytes (chimpanzee)	TYMS	NP_001233511.1
	TYMS	XP_001089274.1
Bos taurus (cattle)	TYMS	NP_001032905.1
Danio rerio (zebrafish)	tyms	NP_571835.1
Kluyveromyces lactis	KLLA0A07843g	XP_451349.1
Arabidopsis thaliana (thale cress)	THY-1	NP_179230.1

Gene Ontology
Bibliography

Related articles in PubMed

5-Fluorouracil targets thymidylate synthase in the selective suppression of TH17 cell differentiation.
Wang J, Peng L, Zhang R, Zheng Z, Chen C, Cheung KL, Cui M, Bian G, Xu F, Chiang D, Hu Y, Chen Y, Lu G, Yang J, Zhang H, Yang J, Zhu H, Chen SH, Liu K, Zhou MM, Sikora AG, Li L, Jiang B, Xiong H
Oncotarget7(15)19312-26(2016 Apr)

A retinoblastoma allele that is mutated at its common E2F interaction site inhibits cell proliferation in gene-targeted mice.
Cecchini MJ, Thwaites MJ, Talluri S, MacDonald JI, Passos DT, Chong JL, Cantalupo P, Stafford PM, S?enz-Robles MT, Francis SM, Pipas JM, Leone G, Welch I, Dick FA
Molecular and cellular biology34(11)2029-45(2014 Jun)

Crystal structure of mouse thymidylate synthase in tertiary complex with dUMP and raltitrexed reveals N-terminus architecture and two different active site conformations.
Dowiercia? A, Wilk P, Rypniewski W, Rode W, Jarmu?a A
BioMed research international2014945803(2014)

Nuclear localization of de novo thymidylate biosynthesis pathway is required to prevent uracil accumulation in DNA.
MacFarlane AJ, Anderson DD, Flodby P, Perry CA, Allen RH, Stabler SP, Stover PJ
The Journal of biological chemistry286(51)44015-22(2011 Dec)

Structure of the gene for mouse thymidylate synthase. Locations of introns and multiple transcriptional start sites.
Deng TL, Li DW, Jenh CH, Johnson LF
The Journal of biological chemistry261(34)16000-5(1986 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that knockdown of thymidylate synthase (TS) significantly impairs TH17 and TH1 cell differentiation.
Title: 5-Fluorouracil targets thymidylate synthase in the selective suppression of TH17 cell differentiation.

Possible interaction routes between hydrophobic residues of the mouse thymidylate synthase protein N-terminal segment and the active site are also discussed
Title: Crystal structure of mouse thymidylate synthase in tertiary complex with dUMP and raltitrexed reveals N-terminus architecture and two different active site conformations.

SHMT1 and TYMS localization to the nucleus is essential to prevent uracil accumulation in DNA
Title: Nuclear localization of de novo thymidylate biosynthesis pathway is required to prevent uracil accumulation in DNA.

posttranslationally-modified thymidylate synthase is associated with cell resistance to 5-fluoro-dUrd
Title: Altered mouse leukemia L1210 thymidylate synthase, associated with cell resistance to 5-fluoro-dUrd, is not mutated but rather reflects posttranslational modification.

downregulation of expression results in block of cell cycle expression when influenced by Fe65
Title: Fe65, a ligand of the Alzheimer's beta-amyloid precursor protein, blocks cell cycle progression by down-regulating thymidylate synthase expression.

The following Tyms gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tyms cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu08556	NM_021288.4 Latest version!	Mus musculus thymidylate synthase (Tyms), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu68998	XM_011249762.2 Latest version!	Mus musculus thymidylate synthase (Tyms), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu68998	XM_017320801.1 Latest version!	Mus musculus thymidylate synthase (Tyms), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tyms ( NM_021288.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_067263.1 Mus musculus thymidylate synthase (Tyms), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu08556
Clone ID Related Accession (Same CDS sequence)	NM_021288.4
Accession Version	NM_021288.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 924bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Mus musculus(house mouse)
Product	thymidylate synthase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC112933.16 and AK035663.1. On Mar 30, 2010 this sequence version replaced NM_021288.3. Transcript Variant: This variant (1) represents the shorter transcript and encodes the functional protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK035663.1, SRR1660811.428980.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164142, SAMN01164143 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGGTGG TTGGCTCCGA GCTGCAGTCC GATGCTCAGC AGCTGAGCGC GGAAGCCCCA 
CGGCATGGAG AACTCCAGTA CCTGAGGCAG GTGGAGCACA TTTTGCGCTG CGGCTTCAAG 
AAGGAGGACC GCACGGGCAC TGGCACCCTG TCGGTGTTCG GCATGCAGGC ACGATACAGC 
CTGAGAGATG AATTTCCTCT GCTCACAACC AAACGAGTGT TCTGGAAGGG TGTTTTGGAG 
GAGTTGTTGT GGTTTATCAA GGGATCCACA AATGCTAAAG AATTGTCCTC AAAGGGAGTG 
AGAATCTGGG ATGCCAATGG ATCCCGAGAT TTTCTGGACA GCTTGGGATT TTCTGCCCGA 
CAGGAAGGGG ACCTGGGCCC AGTTTATGGT TTCCAATGGA GGCATTTTGG AGCAGAGTAC 
AAAGATATGG ATTCAGATTA CTCGGGACAA GGAGTAGACC AGCTGCAAAA AGTGATTGAC 
ACCATCAAAA CCAACCCTGA TGACAGAAGA ATCATCATGT GTGCCTGGAA CCCAAAAGAT 
CTTCCCCTGA TGGCACTGCC TCCTTGCCAT GCCCTCTGTC AGTTCTATGT GGTGAATGGG 
GAACTGTCTT GCCAGCTTTA CCAGAGGTCA GGAGATATGG GTCTGGGCGT GCCCTTCAAC 
ATTGCCAGCT ATGCTCTGCT CACCTACATG ATTGCACATA TCACAGGCCT GCAGCCAGGT 
GATTTTGTCC ACACTTTGGG AGATGCACAT ATTTACCTGA ATCATATAGA GCCGCTGAAA 
ATTCAGCTAC AGCGAGAACC AAGACCTTTC CCAAAGCTCA AAATCCTTCG AAAAGTTGAG 
ACAATCGATG ATTTCAAAGT TGAAGACTTT CAGATTGAAG GGTATAATCC ACATCCAACG 
ATTAAAATGG AAATGGCTGT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067263.1
CDS	79..1002
Translation	MLVVGSELQSDAQQLSAEAPRHGELQYLRQVEHILRCGFKKEDRTGTGTLSVFGMQARYSLRDEFPLLTTKRVFWKGVLEELLWFIKGSTNAKELSSKGVRIWDANGSRDFLDSLGFSARQEGDLGPVYGFQWRHFGAEYKDMDSDYSGQGVDQLQKVIDTIKTNPDDRRIIMCAWNPKDLPLMALPPCHALCQFYVVNGELSCQLYQRSGDMGLGVPFNIASYALLTYMIAHITGLQPGDFVHTLGDAHIYLNHIEPLKIQLQREPRPFPKLKILRKVETIDDFKVEDFQIEGYNPHPTIKMEMAV

Target ORF information:

RefSeq Version	NM_021288.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus thymidylate synthase (Tyms), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021288.4

ORF Insert Sequence:

ATGCTGGTGG TTGGCTCCGA GCTGCAGTCC GATGCTCAGC AGCTGAGCGC GGAAGCCCCA 
CGGCATGGAG AACTCCAGTA CCTGAGGCAG GTGGAGCACA TTTTGCGCTG CGGCTTCAAG 
AAGGAGGACC GCACGGGCAC TGGCACCCTG TCGGTGTTCG GCATGCAGGC ACGATACAGC 
CTGAGAGATG AATTTCCTCT GCTCACAACC AAACGAGTGT TCTGGAAGGG TGTTTTGGAG 
GAGTTGTTGT GGTTTATCAA GGGATCCACA AATGCTAAAG AATTGTCCTC AAAGGGAGTG 
AGAATCTGGG ATGCCAATGG ATCCCGAGAT TTTCTGGACA GCTTGGGATT TTCTGCCCGA 
CAGGAAGGGG ACCTGGGCCC AGTTTATGGT TTCCAATGGA GGCATTTTGG AGCAGAGTAC 
AAAGATATGG ATTCAGATTA CTCGGGACAA GGAGTAGACC AGCTGCAAAA AGTGATTGAC 
ACCATCAAAA CCAACCCTGA TGACAGAAGA ATCATCATGT GTGCCTGGAA CCCAAAAGAT 
CTTCCCCTGA TGGCACTGCC TCCTTGCCAT GCCCTCTGTC AGTTCTATGT GGTGAATGGG 
GAACTGTCTT GCCAGCTTTA CCAGAGGTCA GGAGATATGG GTCTGGGCGT GCCCTTCAAC 
ATTGCCAGCT ATGCTCTGCT CACCTACATG ATTGCACATA TCACAGGCCT GCAGCCAGGT 
GATTTTGTCC ACACTTTGGG AGATGCACAT ATTTACCTGA ATCATATAGA GCCGCTGAAA 
ATTCAGCTAC AGCGAGAACC AAGACCTTTC CCAAAGCTCA AAATCCTTCG AAAAGTTGAG 
ACAATCGATG ATTTCAAAGT TGAAGACTTT CAGATTGAAG GGTATAATCC ACATCCAACG 
ATTAAAATGG AAATGGCTGT TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tyms ( XM_011249762.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011248064.1 Mus musculus thymidylate synthase (Tyms), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu68998
Clone ID Related Accession (Same CDS sequence)	XM_011249762.2 , XM_017320801.1
Accession Version	XM_011249762.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	thymidylate synthase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011249762.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCAG ATTACTCGGG ACAAGGAGTA GACCAGCTGC AAAAAGTGAT TGACACCATC 
AAAACCAACC CTGATGACAG AAGAATCATC ATGTGTGCCT GGAACCCAAA AGATCTTCCC 
CTGATGGCAC TGCCTCCTTG CCATGCCCTC TGTCAGTTCT ATGTGGTGAA TGGGGAACTG 
TCTTGCCAGC TTTACCAGAG GTCAGGAGAT ATGGGTCTGG GCGTGCCCTT CAACATTGCC 
AGCTATGCTC TGCTCACCTA CATGATTGCA CATATCACAG GCCTGCAGCC AGGTGATTTT 
GTCCACACTT TGGGAGATGC ACATATTTAC CTGAATCATA TAGAGCCGCT GAAAATTCAG 
CTACAGCGAG AACCAAGACC TTTCCCAAAG CTCAAAATCC TTCGAAAAGT TGAGACAATC 
GATGATTTCA AAGTTGAAGA CTTTCAGATT GAAGGGTATA ATCCACATCC AACGATTAAA 
ATGGAAATGG CTGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011248064.1
CDS	1711..2208
Translation	MDSDYSGQGVDQLQKVIDTIKTNPDDRRIIMCAWNPKDLPLMALPPCHALCQFYVVNGELSCQLYQRSGDMGLGVPFNIASYALLTYMIAHITGLQPGDFVHTLGDAHIYLNHIEPLKIQLQREPRPFPKLKILRKVETIDDFKVEDFQIEGYNPHPTIKMEMAV

Target ORF information:

RefSeq Version	XM_011249762.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus thymidylate synthase (Tyms), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011249762.2

ORF Insert Sequence:

ATGGATTCAG ATTACTCGGG ACAAGGAGTA GACCAGCTGC AAAAAGTGAT TGACACCATC 
AAAACCAACC CTGATGACAG AAGAATCATC ATGTGTGCCT GGAACCCAAA AGATCTTCCC 
CTGATGGCAC TGCCTCCTTG CCATGCCCTC TGTCAGTTCT ATGTGGTGAA TGGGGAACTG 
TCTTGCCAGC TTTACCAGAG GTCAGGAGAT ATGGGTCTGG GCGTGCCCTT CAACATTGCC 
AGCTATGCTC TGCTCACCTA CATGATTGCA CATATCACAG GCCTGCAGCC AGGTGATTTT 
GTCCACACTT TGGGAGATGC ACATATTTAC CTGAATCATA TAGAGCCGCT GAAAATTCAG 
CTACAGCGAG AACCAAGACC TTTCCCAAAG CTCAAAATCC TTCGAAAAGT TGAGACAATC 
GATGATTTCA AAGTTGAAGA CTTTCAGATT GAAGGGTATA ATCCACATCC AACGATTAAA 
ATGGAAATGG CTGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tyms ( XM_017320801.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017176290.1 Mus musculus thymidylate synthase (Tyms), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu68998
Clone ID Related Accession (Same CDS sequence)	XM_011249762.2 , XM_017320801.1
Accession Version	XM_017320801.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	thymidylate synthase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCAG ATTACTCGGG ACAAGGAGTA GACCAGCTGC AAAAAGTGAT TGACACCATC 
AAAACCAACC CTGATGACAG AAGAATCATC ATGTGTGCCT GGAACCCAAA AGATCTTCCC 
CTGATGGCAC TGCCTCCTTG CCATGCCCTC TGTCAGTTCT ATGTGGTGAA TGGGGAACTG 
TCTTGCCAGC TTTACCAGAG GTCAGGAGAT ATGGGTCTGG GCGTGCCCTT CAACATTGCC 
AGCTATGCTC TGCTCACCTA CATGATTGCA CATATCACAG GCCTGCAGCC AGGTGATTTT 
GTCCACACTT TGGGAGATGC ACATATTTAC CTGAATCATA TAGAGCCGCT GAAAATTCAG 
CTACAGCGAG AACCAAGACC TTTCCCAAAG CTCAAAATCC TTCGAAAAGT TGAGACAATC 
GATGATTTCA AAGTTGAAGA CTTTCAGATT GAAGGGTATA ATCCACATCC AACGATTAAA 
ATGGAAATGG CTGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017176290.1
CDS	440..937
Translation	MDSDYSGQGVDQLQKVIDTIKTNPDDRRIIMCAWNPKDLPLMALPPCHALCQFYVVNGELSCQLYQRSGDMGLGVPFNIASYALLTYMIAHITGLQPGDFVHTLGDAHIYLNHIEPLKIQLQREPRPFPKLKILRKVETIDDFKVEDFQIEGYNPHPTIKMEMAV

Target ORF information:

RefSeq Version	XM_017320801.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus thymidylate synthase (Tyms), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320801.1

ORF Insert Sequence:

ATGGATTCAG ATTACTCGGG ACAAGGAGTA GACCAGCTGC AAAAAGTGAT TGACACCATC 
AAAACCAACC CTGATGACAG AAGAATCATC ATGTGTGCCT GGAACCCAAA AGATCTTCCC 
CTGATGGCAC TGCCTCCTTG CCATGCCCTC TGTCAGTTCT ATGTGGTGAA TGGGGAACTG 
TCTTGCCAGC TTTACCAGAG GTCAGGAGAT ATGGGTCTGG GCGTGCCCTT CAACATTGCC 
AGCTATGCTC TGCTCACCTA CATGATTGCA CATATCACAG GCCTGCAGCC AGGTGATTTT 
GTCCACACTT TGGGAGATGC ACATATTTAC CTGAATCATA TAGAGCCGCT GAAAATTCAG 
CTACAGCGAG AACCAAGACC TTTCCCAAAG CTCAAAATCC TTCGAAAAGT TGAGACAATC 
GATGATTTCA AAGTTGAAGA CTTTCAGATT GAAGGGTATA ATCCACATCC AACGATTAAA 
ATGGAAATGG CTGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

5-Fluorouracil targets thymidylate synthase in the selective suppression of TH17 cell differentiation.
Oncotarget7(15)19312-26(2016 Apr)
Wang J,Peng L,Zhang R,Zheng Z,Chen C,Cheung KL,Cui M,Bian G,Xu F,Chiang D,Hu Y,Chen Y,Lu G,Yang J,Zhang H,Yang J,Zhu H,Chen SH,Liu K,Zhou MM,Sikora AG,Li L,Jiang B,Xiong H

A retinoblastoma allele that is mutated at its common E2F interaction site inhibits cell proliferation in gene-targeted mice.
Molecular and cellular biology34(11)2029-45(2014 Jun)
Cecchini MJ,Thwaites MJ,Talluri S,MacDonald JI,Passos DT,Chong JL,Cantalupo P,Stafford PM,S?enz-Robles MT,Francis SM,Pipas JM,Leone G,Welch I,Dick FA

Crystal structure of mouse thymidylate synthase in tertiary complex with dUMP and raltitrexed reveals N-terminus architecture and two different active site conformations.
BioMed research international2014945803(2014)
Dowiercia? A,Wilk P,Rypniewski W,Rode W,Jarmu?a A

Nuclear localization of de novo thymidylate biosynthesis pathway is required to prevent uracil accumulation in DNA.
The Journal of biological chemistry286(51)44015-22(2011 Dec)
MacFarlane AJ,Anderson DD,Flodby P,Perry CA,Allen RH,Stabler SP,Stover PJ

Structure of the gene for mouse thymidylate synthase. Locations of introns and multiple transcriptional start sites.
The Journal of biological chemistry261(34)16000-5(1986 Dec)
Deng TL,Li DW,Jenh CH,Johnson LF

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Tyms cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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