Tssk1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tssk1
Entrez Gene ID	22114
Full Name	testis-specific serine kinase 1
Synonyms	Stk22a,TSK-1,Tsk1,Tssk,Tssk1b
General protein information	Preferred Names testis-specific serine kinase 1 Names testis-specific serine/threonine-protein kinase 1 TSSK-1 serine/threonine kinase 22A (spermiogenesis associated) serine/threonine-protein kinase 22A testis-specific kinase 1 testis-specific serine/threonine kinase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 16 Map Location: 16 A3\|16 11.09 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_009435.2	NP_033461.2	testis-specific serine/threonine-protein kinase 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TSSK1B	XP_001145095.1
Bos taurus (cattle)	TSSK1B	NP_001077179.1
Canis lupus familiaris (dog)	TSSK1B	XP_543553.1
Rattus norvegicus (Norway rat)	Tssk1b	NP_001011900.1
Drosophila melanogaster (fruit fly)	CG14305	NP_650732.1
Homo sapiens (human)	TSSK1B	NP_114417.1
Mus musculus (house mouse)	Tssk1	NP_033461.2
Caenorhabditis elegans (roundworm)	CELE_C27D6.11	NP_001021995.1
Caenorhabditis elegans (roundworm)	tag-344	NP_492786.1
	TSSK1B	XP_002804522.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010341	XP_001230821.2
Caenorhabditis elegans (roundworm)	CELE_Y38H8A.4	NP_502595.1

Gene Ontology
Bibliography

Related articles in PubMed

PPP1CC2 can form a kinase/phosphatase complex with the testis-specific proteins TSSK1 and TSKS in the mouse testis.
MacLeod G, Shang P, Booth GT, Mastropaolo LA, Manafpoursakha N, Vogl AW, Varmuza S
Reproduction (Cambridge, England)147(1)1-12(2014 Jan)

Heat shock protein 90 functions to stabilize and activate the testis-specific serine/threonine kinases, a family of kinases essential for male fertility.
Jha KN, Coleman AR, Wong L, Salicioni AM, Howcroft E, Johnson GR
The Journal of biological chemistry288(23)16308-20(2013 Jun)

[Cloning and expression of Tssk1 & Tssk2 in mice and the presence & localization of them in mature sperm].
Yang MH, Su JZ, Liu J, Li YH
Dong wu xue yan jiu = Zoological research33(4)381-8(2012 Aug)

Expression and localization of five members of the testis-specific serine kinase (Tssk) family in mouse and human sperm and testis.
Li Y, Sosnik J, Brassard L, Reese M, Spiridonov NA, Bates TC, Johnson GR, Anguita J, Visconti PE, Salicioni AM
Molecular human reproduction17(1)42-56(2011 Jan)

Functional transformation of the chromatoid body in mouse spermatids requires testis-specific serine/threonine kinases.
Shang P, Baarends WM, Hoogerbrugge J, Ooms MP, van Cappellen WA, de Jong AA, Dohle GR, van Eenennaam H, Gossen JA, Grootegoed JA
Journal of cell science123(Pt 3)331-9(2010 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In mouse testis, PPP1CC2 can form a complex with TSSK1 mediated by the direct interaction of each with the kinase substrate protein TSKS. Interaction between PPP1CC2 and TSKS is mediated through an RVxF docking motif on the TSKS surface.
Title: PPP1CC2 can form a kinase/phosphatase complex with the testis-specific proteins TSSK1 and TSKS in the mouse testis.

HSP90 function abolished the catalytic activities of TSSK4 and -6 but did not significantly alter the specific activities of TSSK1 and -2. Inhibition of HSP90 resulted in increased TSSK ubiquitination and proteasomal degradation
Title: Heat shock protein 90 functions to stabilize and activate the testis-specific serine/threonine kinases, a family of kinases essential for male fertility.

Tssk1 and Tssk2 play an important role in the regulation of sperm function.
Title: [Cloning and expression of Tssk1 & Tssk2 in mice and the presence & localization of them in mature sperm].

Results reveal TSSK1- and TSSK2-dependent functions of a transformed chromatoid body in post-meiotic cytodifferentiation of spermatids.
Title: Functional transformation of the chromatoid body in mouse spermatids requires testis-specific serine/threonine kinases.

Results confirm both TSSK 2 and TSKS persist in sperm, define a critical role of TSKS' N-terminus in enzyme interaction, identify Ser 281 as a TSKS phosphorylation site and indicate an indispensable role for TSSK 1 and 2 in spermiogenesis.
Title: Targeted deletion of Tssk1 and 2 causes male infertility due to haploinsufficiency.

The following Tssk1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tssk1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu20659	NM_009435.2 Latest version!	Mus musculus testis-specific serine kinase 1 (Tssk1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tssk1 ( NM_009435.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033461.2 Mus musculus testis-specific serine kinase 1 (Tssk1), mRNA.

Price & Availability↑

CloneID	OMu20659
Clone ID Related Accession (Same CDS sequence)	NM_009435.2
Accession Version	NM_009435.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1098bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Mus musculus(house mouse)
Product	testis-specific serine/threonine-protein kinase 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK006517.1, BC050772.1 and U01840.1. On Aug 18, 2006 this sequence version replaced NM_009435.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. COMPLETENESS: complete on the 3' end.

ATGGATGACG CTGCCGTCCT CAAGCGACGA GGCTACATCA TGGGGATAAA CTTGGGAGAG 
GGCTCATATG CCAAAGTCAA ATCCGCTTAC TCTGAGCGCC TAAAGTTCAA CGTGGCGGTC 
AAGATCATCG ATCGCAAGAA AGCCCCCTCA GACTTCCTGG AGAAATTCCT TCCCCGGGAG 
ATTGAGATTC TGGCCATGCT AAACCACCGC TCTATTGTCA AGACCTATGA GATCTTTGAG 
ACATCAGATG GCAAGGTCTA CATTGTCATG GAGCTTGGGG TCCAGGGTGA CCTCCTCGAG 
TTCATCAAAA CCAGAGGAGC CCTGCAAGAG GATGATGCTC GAAAGAAGTT CCACCAGCTC 
TCCTCGGCCA TCAAGTACTG CCACGACCTG GATGTCGTCC ACCGAGACCT CAAATGTGAG 
AACCTTCTGC TTGACAAGGA CTTCAACATC AAACTGTCTG ACTTCGGCTT CTCCAAGCGC 
TGCTTGCGGG ATGATAGTGG TCGACTGATA TTAAGCAAGA CCTTCTGTGG GTCAGCAGCT 
TATGCGGCCC CCGAGGTGCT GCAGGGCATC CCCTACCAGC CCAAGGTGTA TGACATCTGG 
AGCCTGGGTG TGATTCTCTA CATCATGGTC TGTGGCTCCA TGCCCTACGA TGACTCCAAC 
ATCAAAAAGA TGCTGCGCAT CCAGAAGGAG CACCGTGTCA ACTTTCCACG CTCTAAACAC 
CTGACTGGTG AGTGCAAGGA CCTCATCTAC AGAATGTTAC AGCCAGATGT CAACCGCCGG 
CTGCACATCG ATGAGATCCT TAACCACTGC TGGGTGCAGC CCAAGGCACG AGGTCTGTCC 
TCTGGAGCCA TCAACAAGGA GGGGGAAAGC TCCCGGGCCA CTGAGCCCTC ATGGATCCCT 
GAACCTGGTG CTGATAAGAA GTCTGCCACC AAGTTGGAGC CTAGGGAAGA GGCACGATCT 
GAGGCCCGGT CTGAGTCAAA ACCCCAGGAA GATACTTTGC AGGTGGTCAG GCAGTCAGAG 
AACGTGGGCC TCAGCAGCGA ACTGAACAGG GACACAGAGG AAGGGCACCC CCAACAGCCT 
TCAGAGACAC ATACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033461.2
CDS	151..1248
Translation	MDDAAVLKRRGYIMGINLGEGSYAKVKSAYSERLKFNVAVKIIDRKKAPSDFLEKFLPREIEILAMLNHRSIVKTYEIFETSDGKVYIVMELGVQGDLLEFIKTRGALQEDDARKKFHQLSSAIKYCHDLDVVHRDLKCENLLLDKDFNIKLSDFGFSKRCLRDDSGRLILSKTFCGSAAYAAPEVLQGIPYQPKVYDIWSLGVILYIMVCGSMPYDDSNIKKMLRIQKEHRVNFPRSKHLTGECKDLIYRMLQPDVNRRLHIDEILNHCWVQPKARGLSSGAINKEGESSRATEPSWIPEPGADKKSATKLEPREEARSEARSESKPQEDTLQVVRQSENVGLSSELNRDTEEGHPQQPSETHT

Target ORF information:

RefSeq Version	NM_009435.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus testis-specific serine kinase 1 (Tssk1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009435.2

ORF Insert Sequence:

ATGGATGACG CTGCCGTCCT CAAGCGACGA GGCTACATCA TGGGGATAAA CTTGGGAGAG 
GGCTCATATG CCAAAGTCAA ATCCGCTTAC TCTGAGCGCC TAAAGTTCAA CGTGGCGGTC 
AAGATCATCG ATCGCAAGAA AGCCCCCTCA GACTTCCTGG AGAAATTCCT TCCCCGGGAG 
ATTGAGATTC TGGCCATGCT AAACCACCGC TCTATTGTCA AGACCTATGA GATCTTTGAG 
ACATCAGATG GCAAGGTCTA CATTGTCATG GAGCTTGGGG TCCAGGGTGA CCTCCTCGAG 
TTCATCAAAA CCAGAGGAGC CCTGCAAGAG GATGATGCTC GAAAGAAGTT CCACCAGCTC 
TCCTCGGCCA TCAAGTACTG CCACGACCTG GATGTCGTCC ACCGAGACCT CAAATGTGAG 
AACCTTCTGC TTGACAAGGA CTTCAACATC AAACTGTCTG ACTTCGGCTT CTCCAAGCGC 
TGCTTGCGGG ATGATAGTGG TCGACTGATA TTAAGCAAGA CCTTCTGTGG GTCAGCAGCT 
TATGCGGCCC CCGAGGTGCT GCAGGGCATC CCCTACCAGC CCAAGGTGTA TGACATCTGG 
AGCCTGGGTG TGATTCTCTA CATCATGGTC TGTGGCTCCA TGCCCTACGA TGACTCCAAC 
ATCAAAAAGA TGCTGCGCAT CCAGAAGGAG CACCGTGTCA ACTTTCCACG CTCTAAACAC 
CTGACTGGTG AGTGCAAGGA CCTCATCTAC AGAATGTTAC AGCCAGATGT CAACCGCCGG 
CTGCACATCG ATGAGATCCT TAACCACTGC TGGGTGCAGC CCAAGGCACG AGGTCTGTCC 
TCTGGAGCCA TCAACAAGGA GGGGGAAAGC TCCCGGGCCA CTGAGCCCTC ATGGATCCCT 
GAACCTGGTG CTGATAAGAA GTCTGCCACC AAGTTGGAGC CTAGGGAAGA GGCACGATCT 
GAGGCCCGGT CTGAGTCAAA ACCCCAGGAA GATACTTTGC AGGTGGTCAG GCAGTCAGAG 
AACGTGGGCC TCAGCAGCGA ACTGAACAGG GACACAGAGG AAGGGCACCC CCAACAGCCT 
TCAGAGACAC ATACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

PPP1CC2 can form a kinase/phosphatase complex with the testis-specific proteins TSSK1 and TSKS in the mouse testis.
Reproduction (Cambridge, England)147(1)1-12(2014 Jan)
MacLeod G,Shang P,Booth GT,Mastropaolo LA,Manafpoursakha N,Vogl AW,Varmuza S

Heat shock protein 90 functions to stabilize and activate the testis-specific serine/threonine kinases, a family of kinases essential for male fertility.
The Journal of biological chemistry288(23)16308-20(2013 Jun)
Jha KN,Coleman AR,Wong L,Salicioni AM,Howcroft E,Johnson GR

[Cloning and expression of Tssk1 & Tssk2 in mice and the presence & localization of them in mature sperm].
Dong wu xue yan jiu = Zoological research33(4)381-8(2012 Aug)
Yang MH,Su JZ,Liu J,Li YH

Expression and localization of five members of the testis-specific serine kinase (Tssk) family in mouse and human sperm and testis.
Molecular human reproduction17(1)42-56(2011 Jan)
Li Y,Sosnik J,Brassard L,Reese M,Spiridonov NA,Bates TC,Johnson GR,Anguita J,Visconti PE,Salicioni AM

Functional transformation of the chromatoid body in mouse spermatids requires testis-specific serine/threonine kinases.
Journal of cell science123(Pt 3)331-9(2010 Feb)
Shang P,Baarends WM,Hoogerbrugge J,Ooms MP,van Cappellen WA,de Jong AA,Dohle GR,van Eenennaam H,Gossen JA,Grootegoed JA

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Tssk1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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