gRNAs
The following Top3a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Top3a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OMu68937 | |
| Clone ID Related Accession (Same CDS sequence) | XM_017314499.1 , XM_017314499.2 | |
| Accession Version | XM_017314499.1 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2835bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-06-21 | |
| Organism | Mus musculus(house mouse) | |
| Product | DNA topoisomerase 3-alpha isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGAAAGAAG GCCTTTCTAA ATTCAACAAG ATTTATGAAT TTGACTATCA TCTGTATGGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_017169988.1 |
| CDS | 19..2853 |
| Translation |
Target ORF information:
Target ORF information:
|
 XM_017314499.1 |
1 | ATGAAAGAAG GCCTTTCTAA ATTCAACAAG ATTTATGAAT TTGACTATCA TCTGTATGGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu68937 | |
| Clone ID Related Accession (Same CDS sequence) | XM_017314499.1 , XM_017314499.2 | |
| Accession Version | XM_017314499.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2835bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-08-07 | |
| Organism | Mus musculus(house mouse) | |
| Product | DNA topoisomerase 3-alpha isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017314499.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGAAAGAAG GCCTTTCTAA ATTCAACAAG ATTTATGAAT TTGACTATCA TCTGTATGGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_017169988.1 |
| CDS | 18..2852 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_017314499.2 |
1 | ATGAAAGAAG GCCTTTCTAA ATTCAACAAG ATTTATGAAT TTGACTATCA TCTGTATGGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu04085 | |
| Clone ID Related Accession (Same CDS sequence) | NM_009410.3 , NM_009410.2 | |
| Accession Version | NM_009410.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3012bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-08-10 | |
| Organism | Mus musculus(house mouse) | |
| Product | DNA topoisomerase 3-alpha | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL596215.7. On Feb 24, 2018 this sequence version replaced NM_009410.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AB006074.1, BC099689.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGATCTTCC CGGTCACCCT CTTAGCGTTT CAGTGGCACC GACGGCCTGG AGGCCGTGCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_033436.1 |
| CDS | 265..3276 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_009410.3 |
1 | ATGATCTTCC CGGTCACCCT CTTAGCGTTT CAGTGGCACC GACGGCCTGG AGGCCGTGCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu04085 | |
| Clone ID Related Accession (Same CDS sequence) | NM_009410.3 , NM_009410.2 | |
| Accession Version | NM_009410.2 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3012bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-09-30 | |
| Organism | Mus musculus(house mouse) | |
| Product | DNA topoisomerase 3-alpha | |
| Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB006074.1. On Apr 6, 2007 this sequence version replaced NM_009410.1. ##Evidence-Data-START## Transcript exon combination :: AB006074.1, BC099689.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## | |
1 | ATGATCTTCC CGGTCACCCT CTTAGCGTTT CAGTGGCACC GACGGCCTGG AGGCCGTGCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_033436.1 |
| CDS | 265..3276 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_009410.2 |
1 | ATGATCTTCC CGGTCACCCT CTTAGCGTTT CAGTGGCACC GACGGCCTGG AGGCCGTGCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Penetrance of craniofacial anomalies in mouse models of Smith-Magenis syndrome is modified by genomic sequence surrounding Rai1: not all null alleles are alike. |
A genome-wide in situ hybridization map of RNA-binding proteins reveals anatomically restricted expression in the developing mouse brain. |
BayGenomics: a resource of insertional mutations in mouse embryonic stem cells. |
Regulation of mouse DNA topoisomerase IIIalpha gene expression by YY1 and USF transcription factors. |
Isolation of a cDNA encoding mouse DNA topoisomerase III which is highly expressed at the mRNA level in the testis. |
