Cd70 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cd70
Entrez Gene ID	21948
Full Name	CD70 antigen
Synonyms	CD27LG,Cd27l,Tnfsf7,Tnlg8a
General protein information	Preferred Names CD70 antigen Names CD70 antigen CD27 ligand CD27-L Ki-24 antigen tumor necrosis factor ligand 8a tumor necrosis factor ligand superfamily member 7
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17 D\|17 29.69 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_011617.2	NP_035747.1	CD70 antigen

Pathways from BioSystems

Homologies

Bos taurus (cattle)	CD70	XP_002688954.3
Mus musculus (house mouse)	Cd70	NP_035747.1
	CD70	XP_001088935.1
Canis lupus familiaris (dog)	CD70	XP_542136.3
Rattus norvegicus (Norway rat)	Cd70	NP_001100348.1
Homo sapiens (human)	CD70	NP_001243.1

Gene Ontology
Bibliography

Related articles in PubMed

CD70, a novel target of CAR T-cell therapy for gliomas.
Jin L, Ge H, Long Y, Yang C, Chang YE, Mu L, Sayour EJ, De Leon G, Wang QJ, Yang JC, Kubilis PS, Bao H, Xia S, Lu D, Kong Y, Hu L, Shang Y, Jiang C, Nie J, Li S, Gu Y, Sun J, Mitchell DA, Lin Z, Huang J
Neuro-oncology20(1)55-65(2018 Jan)

T Cell-Derived CD70 Delivers an Immune Checkpoint Function in Inflammatory T Cell Responses.
O'Neill RE, Du W, Mohammadpour H, Alqassim E, Qiu J, Chen G, McCarthy PL, Lee KP, Cao X
Journal of immunology (Baltimore, Md. : 1950)199(10)3700-3710(2017 Nov)

CD70 reverse signaling enhances NK cell function and immunosurveillance in CD27-expressing B-cell malignancies.
Al Sayed MF, Ruckstuhl CA, Hilmenyuk T, Claus C, Bourquin JP, Bornhauser BC, Radpour R, Riether C, Ochsenbein AF
Blood130(3)297-309(2017 07)

Host-Derived CD70 Suppresses Murine Graft-versus-Host Disease by Limiting Donor T Cell Expansion and Effector Function.
Leigh ND, O'Neill RE, Du W, Chen C, Qiu J, Ashwell JD, McCarthy PL, Chen GL, Cao X
Journal of immunology (Baltimore, Md. : 1950)199(1)336-347(2017 Jul)

Gut symbiotic microbes imprint intestinal immune cells with the innate receptor SLAMF4 which contributes to gut immune protection against enteric pathogens.
Cabinian A, Sinsimer D, Tang M, Jang Y, Choi B, Laouar Y, Laouar A
Gut(2017 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

these findings demonstrate for the first time, to our knowledge, that T cell-derived CD70 plays a novel immune checkpoint role in inhibiting inflammatory T cell responses
Title: T Cell-Derived CD70 Delivers an Immune Checkpoint Function in Inflammatory T Cell Responses.

CD70 reverse signaling enhances NK cell function and immunosurveillance in CD27-expressing B-cell malignancies.
Title: CD70 reverse signaling enhances NK cell function and immunosurveillance in CD27-expressing B-cell malignancies.

findings demonstrate that host CD70 serves as a unique negative regulator of allogeneic T cell response by contributing to donor T cell apoptosis and inhibiting expansion of donor effector T cells.
Title: Host-Derived CD70 Suppresses Murine Graft-versus-Host Disease by Limiting Donor T Cell Expansion and Effector Function.

Mice lacking CD70 did not accumulate memory T cells and did not develop hypertension.
Title: CD70 Exacerbates Blood Pressure Elevation and Renal Damage in Response to Repeated Hypertensive Stimuli.

find that while stimulation of CD27 in isolation drives weak Eomesodermin(hi) T-bet(lo) CD8(+) T-cell responses to OVA immunization
Title: CD70 and IFN-1 selectively induce eomesodermin or T-bet and synergize to promote CD8+ T-cell responses.

The following Cd70 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cd70 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu08463	NM_011617.2 Latest version!	Mus musculus CD70 antigen (Cd70), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cd70 ( NM_011617.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035747.1 Mus musculus CD70 antigen (Cd70), mRNA.

Price & Availability↑

CloneID	OMu08463
Clone ID Related Accession (Same CDS sequence)	NM_011617.2
Accession Version	NM_011617.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 588bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Mus musculus(house mouse)
Product	CD70 antigen
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CT025692.15. On Mar 21, 2007 this sequence version replaced NM_011617.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Y13636.1, BC125407.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849383 [ECO:0000348] ##Evidence-Data-END##

ATGCCGGAGG AAGGTCGCCC TTGCCCCTGG GTTCGCTGGA GCGGGACCGC GTTCCAGCGC 
CAATGGCCAT GGCTGCTGCT GGTGGTGTTT ATTACTGTGT TTTGCTGTTG GTTTCATTGT 
AGCGGACTAC TCAGTAAGCA GCAACAGAGG CTGCTGGAGC ACCCTGAGCC GCACACAGCT 
GAGTTACAGC TGAATCTCAC AGTTCCTCGG AAGGACCCCA CACTGCGCTG GGGAGCAGGC 
CCAGCCTTGG GAAGGTCCTT CACACACGGA CCAGAGCTGG AGGAGGGCCA TCTGCGTATC 
CATCAAGATG GCCTCTACAG GCTGCATATC CAGGTGACAC TGGCCAACTG CTCTTCCCCA 
GGCAGCACCC TGCAGCACAG GGCCACCCTG GCTGTGGGCA TCTGCTCCCC CGCTGCGCAC 
GGCATCAGCT TGCTGCGTGG GCGCTTTGGA CAGGACTGTA CAGTGGCATT ACAGCGCCTG 
ACATACCTGG TCCACGGAGA TGTCCTCTGT ACCAACCTCA CCCTGCCTCT GCTGCCGTCC 
CGCAACGCTG ATGAGACCTT CTTTGGAGTT CAGTGGATAT GCCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035747.1
CDS	177..764
Translation	MPEEGRPCPWVRWSGTAFQRQWPWLLLVVFITVFCCWFHCSGLLSKQQQRLLEHPEPHTAELQLNLTVPRKDPTLRWGAGPALGRSFTHGPELEEGHLRIHQDGLYRLHIQVTLANCSSPGSTLQHRATLAVGICSPAAHGISLLRGRFGQDCTVALQRLTYLVHGDVLCTNLTLPLLPSRNADETFFGVQWICP

Target ORF information:

RefSeq Version	NM_011617.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus CD70 antigen (Cd70), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011617.2

ORF Insert Sequence:

ATGCCGGAGG AAGGTCGCCC TTGCCCCTGG GTTCGCTGGA GCGGGACCGC GTTCCAGCGC 
CAATGGCCAT GGCTGCTGCT GGTGGTGTTT ATTACTGTGT TTTGCTGTTG GTTTCATTGT 
AGCGGACTAC TCAGTAAGCA GCAACAGAGG CTGCTGGAGC ACCCTGAGCC GCACACAGCT 
GAGTTACAGC TGAATCTCAC AGTTCCTCGG AAGGACCCCA CACTGCGCTG GGGAGCAGGC 
CCAGCCTTGG GAAGGTCCTT CACACACGGA CCAGAGCTGG AGGAGGGCCA TCTGCGTATC 
CATCAAGATG GCCTCTACAG GCTGCATATC CAGGTGACAC TGGCCAACTG CTCTTCCCCA 
GGCAGCACCC TGCAGCACAG GGCCACCCTG GCTGTGGGCA TCTGCTCCCC CGCTGCGCAC 
GGCATCAGCT TGCTGCGTGG GCGCTTTGGA CAGGACTGTA CAGTGGCATT ACAGCGCCTG 
ACATACCTGG TCCACGGAGA TGTCCTCTGT ACCAACCTCA CCCTGCCTCT GCTGCCGTCC 
CGCAACGCTG ATGAGACCTT CTTTGGAGTT CAGTGGATAT GCCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CD70, a novel target of CAR T-cell therapy for gliomas.
Neuro-oncology20(1)55-65(2018 Jan)
Jin L,Ge H,Long Y,Yang C,Chang YE,Mu L,Sayour EJ,De Leon G,Wang QJ,Yang JC,Kubilis PS,Bao H,Xia S,Lu D,Kong Y,Hu L,Shang Y,Jiang C,Nie J,Li S,Gu Y,Sun J,Mitchell DA,Lin Z,Huang J

T Cell-Derived CD70 Delivers an Immune Checkpoint Function in Inflammatory T Cell Responses.
Journal of immunology (Baltimore, Md. : 1950)199(10)3700-3710(2017 Nov)
O'Neill RE,Du W,Mohammadpour H,Alqassim E,Qiu J,Chen G,McCarthy PL,Lee KP,Cao X

CD70 reverse signaling enhances NK cell function and immunosurveillance in CD27-expressing B-cell malignancies.
Blood130(3)297-309(2017 07)
Al Sayed MF,Ruckstuhl CA,Hilmenyuk T,Claus C,Bourquin JP,Bornhauser BC,Radpour R,Riether C,Ochsenbein AF

Host-Derived CD70 Suppresses Murine Graft-versus-Host Disease by Limiting Donor T Cell Expansion and Effector Function.
Journal of immunology (Baltimore, Md. : 1950)199(1)336-347(2017 Jul)
Leigh ND,O'Neill RE,Du W,Chen C,Qiu J,Ashwell JD,McCarthy PL,Chen GL,Cao X

Gut symbiotic microbes imprint intestinal immune cells with the innate receptor SLAMF4 which contributes to gut immune protection against enteric pathogens.
Gut(2017 Mar)
Cabinian A,Sinsimer D,Tang M,Jang Y,Choi B,Laouar Y,Laouar A