Vwa8 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vwa8
Entrez Gene ID	219189
Full Name	von Willebrand factor A domain containing 8
Synonyms	1300010F03Rik,4932416F07Rik,AI957255,Kiaa0564,mKIAA0564
General protein information	Preferred Names von Willebrand factor A domain containing 8 Names von Willebrand factor A domain-containing protein 8
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 14 Map Location: 14\|14 D3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006518904.3	XP_006518967.1	von Willebrand factor A domain-containing protein 8 isoform X2
XM_011245038.2	XP_011243340.1	von Willebrand factor A domain-containing protein 8 isoform X3
XM_017315982.1	XP_017171471.1	von Willebrand factor A domain-containing protein 8 isoform X1
NM_173758.3	NP_776119.2	von Willebrand factor A domain-containing protein 8 isoform 2 precursor
NM_027906.1	NP_082182.1	von Willebrand factor A domain-containing protein 8 isoform 1 precursor
XM_011245038.3	XP_011243340.1	von Willebrand factor A domain-containing protein 8 isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	VWA8	NP_055873.1
Mus musculus (house mouse)	Vwa8	NP_082182.1
	VWA8	XP_001091849.2
Bos taurus (cattle)	VWA8	XP_002691869.1
Canis lupus familiaris (dog)	VWA8	XP_005633992.1
Gallus gallus (chicken)	KIAA0564	XP_425624.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000545	XP_310539.4
Caenorhabditis elegans (roundworm)	F11C1.5	NP_001257211.1
Pan troglodytes (chimpanzee)	VWA8	XP_003318070.1
Danio rerio (zebrafish)	vwa8	NP_001121810.1
Drosophila melanogaster (fruit fly)	c12.2	NP_652604.2
Xenopus tropicalis (tropical clawed frog)	vwa8	NP_001096540.1

Gene Ontology
Bibliography

Related articles in PubMed

Characterization of the novel protein KIAA0564 (Von Willebrand Domain-containing Protein 8).
Luo M, Mengos AE, Ma W, Finlayson J, Bustos RZ, Xiao Zhu Y, Shi CX, Stubblefield TM, Willis WT, Mandarino LJ
Biochemical and biophysical research communications487(3)545-551(2017 06)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Integrating genetic and network analysis to characterize genes related to mouse weight.
Ghazalpour A, Doss S, Zhang B, Wang S, Plaisier C, Castellanos R, Brozell A, Schadt EE, Drake TA, Lusis AJ, Horvath S
PLoS genetics2(8)e130(2006 Aug)

Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.
Mootha VK, Bunkenborg J, Olsen JV, Hjerrild M, Wisniewski JR, Stahl E, Bolouri MS, Ray HN, Sihag S, Kamal M, Patterson N, Lander ES, Mann M
Cell115(5)629-40(2003 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The VWA8 protein was targeted exclusively to mitochondria in mouse AML12 liver cells, and this was prevented by deletion of the targeting sequence.
Title: Characterization of the novel protein KIAA0564 (Von Willebrand Domain-containing Protein 8).

The following Vwa8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vwa8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu73110	XM_011245038.3 Latest version!	Mus musculus von Willebrand factor A domain containing 8 (Vwa8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu78740	XM_017315982.1 Latest version!	Mus musculus von Willebrand factor A domain containing 8 (Vwa8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu03757	NM_173758.3 Latest version!	Mus musculus von Willebrand factor A domain containing 8 (Vwa8), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu04758	NM_027906.1 Latest version!	Mus musculus von Willebrand factor A domain containing 8 (Vwa8), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu49915	XM_006518904.3 Latest version!	Mus musculus von Willebrand factor A domain containing 8 (Vwa8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
View Old Accession Versions >>
OMu73110	XM_011245038.2	Mus musculus von Willebrand factor A domain containing 8 (Vwa8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vwa8 ( XM_011245038.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011243340.1 Mus musculus von Willebrand factor A domain containing 8 (Vwa8), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu73110
Clone ID Related Accession (Same CDS sequence)	XM_011245038.2 , XM_011245038.3
Accession Version	XM_011245038.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	von Willebrand factor A domain-containing protein 8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011245038.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCAC GCCTCCTCCT CCTAGGAGCC CCGGGCGGCC TTGGGGACGT GGCCTCCCGG 
CGAGTGCGGC TGCTCTTGCG GCAGGTGTTG CGGGGCAGGC CGGGCGGGGA CCAGCAGCGG 
CTGGAGGTCA GGCTGCTGCA CTCTGGGGCG ACCGACTCAG GTGAAACGGT TAGTATTGGA 
GATGTGTCCT ACAAGCTGAA AACTCCCAAG AATCCAGAAC TTGTCCCACA GAACTACATT 
TCAGACTCTC CAGCTCAGTC CATAGTCCAG CATCTGAGAT GGCTGATGCA GAAGGATCTG 
CTGGGGCAGG ATGTCTTTCT CATCGGACCT CCTGGGCCTC TCCGGCGCTC GGTGGCTATG 
CAGTACTTGG AGCTGACCAA ACGAGAGGTG GAATACATCG CCCTGTCAAG GGACACCACT 
GAAACTGACC TCAAACAGCG CCGGGAGATC CGAGCTGGCA CAGCCTTTTA CATTGACCAG 
TGTGCGGTTC GGGCTGCCAC AGAAGGCAGG ACGCTGGTTT TAGAAGGCTT GGAAAAGGCG 
GAGAGAAACG TGCTTCCAGT ACTGAACAAC TTGCTGGAGA ACCGGGAGAT GCAGCTTGAA 
GATGGGCGCT TCCTCATGTC TGCGGAGCGC TATGACAAGC TTCTCCAAGA TCACACTAAA 
GAGGAGCTGG ACGCGTGGAA GATTGTCCGA GTCAGTGAGA ATTTCCGAGT GATTGCCTTG 
GGCTTGCCAG TGCCCAGGTA CTCTGGGAAT CCATTAGACC CCCCTCTCCG GTCTCGATTT 
CAAGCCAGAG ATATCTATTT TCTACCCTTT CAGGACCAAC TAAAACTATT GTATTCAGTT 
GGAGCCAATG TTTCTGCTGA GAAGATTTCT CAGCTCTTGT CCTTTGCGAC AACCCTCTGC 
TCCCAAGAAT CCTCTACGCT TGGGCTTCCA GACTTTCCCT TAGATAGTTT GCCAGAGGCA 
GTTCAAATCC TGGACTCCTT TCCCATGATG TCGATTGAAC ATGCACTCCA GTGGGTGTAT 
CCTTACACTC TTTTACTCGG ACACGAGGGG AAGATGGCCG TGGAGGGTGT TTTAAAACGC 
TTTGAGCTCC AAGGTTCTGG ACATTCCCTG CTTCCTAAGG AGATTGTAAG AGTGGAGAGG 
ATGACTGACA GCCACGGCTC CTATGCCCAC GTGACAATCC GGGTCGCAGG AAAAGAGGTG 
ACCATTAAGG TACCAGCGGG GACCAGAGCA GTAAACCAGC CTTGTGCTCC TGACCATTTC 
ATTCAGACTG TCAGTCACAA GCAATTACTG GCTGAGATGG TGCAGTCTCA TATGGTGAAG 
GACATATGCT TAATTGGAGG GAAGGGCTGT GGGAAAACGG TCATCGCTAA GAACTTTGCT 
GCTCTCCTAG GATACAGCAT AGAACCCATC ATGCTTTACC AGGATATGAC TGCGCGGGAC 
CTGCTACAGC AGCGGTACAC GCTCCCCAAC GGCGACACCG CCTGGCGCTC CTCACCCCTT 
GTGAGTGCTG CCCGGGAAGG CAAACTGGTC CTGTTGGATG GTATCCACCG CGTCAACGCT 
GGCACACTGG CTGTATTGCA AAGACTGATC CATGACCGGG AGCTCAGCCT GTATGATGGA 
TCTCGGCTGC TGAGGGAGGA CAGGTACCTG AGCTTGAAGG AGAAGCTACA ACTGACCGAT 
GAGCAGCTAC AGAACAGGTC CATTTTTCCT ATCCACCCTT CCTTCAGAAT CATTGCGTTG 
GCAGAGCCCC CAATTGTTGG AAGCACAACA CAGCAGTGGC TGGGACCAGA ATTTTTAACT 
ATGTTCTTTT TCCATCACAT GAAGCCTCTT GTGAAAAGTG AAGAAATCCA AGTGATAAAG 
GAGACGGTTC CAAATGTACC TCAGGAAGCA CTGGAGAAAT TGCTGTCAGT CACTCACAAA 
CTCAGGGAGA CCCAGGATCC CACAGCACAG TCCTTGGCAG CCTCGCTTTC CACCCGTCAG 
CTCTTACGGA TCTCTCGCCG GCTCTCGAAG TATCCCAGCG AAAATCTTCA TGATGCCATT 
ACCAAAGCCT GTCTTTCCAG GTTCTTACCA AGCCTTGCTC AGTCTGCACT GGAGAAGAAT 
CTGGCAGATG CGGCAATAGA AACAAACACT GAGGACAGCC TGGAGCCAGA GCTGGAGAAT 
TACAAGTGTG AAGTAGTCGC CGGGTCTCTG AAGATTGGCG CCGTCAGTGT GCCAGTGCAC 
AATGCCCATG AGAAGATGAA AGTGCCTGAC GTTCTTTTCT ACGACAATGT TCAGCACATG 
GTAGTGATGG AAGACATGCT TAAAGACTTT GTCCTTGGCG AACACTTACT ATTGGTTGGT 
AACCAGGGGG TAGGAAAGAA CAAGATTGTG GACCGATTCC TTCACCTGCT CAACAGACCC 
CGAGAATATA TACAGCTGCA CAGGGACACC ACAGTGCAGT CTCTGACTCT CCAGCCCACA 
GTGAAAGGTG GACTGATTGT ATATGAAGAC TCACCTCTGG TTAAAGCAGT AAAGTTGGGT 
CATATTCTAG TAGTAGATGA GGCAGACAAA GCCCCAACAA ATGTCACCTG TATTTTGAAG 
ACTCTGGTAG AAAACGGAGA AATGATTCTT GCGGACGGAA GACGTATTGT TGCAGATGCA 
GCAAATGTGG ATGGAAGAGA GAACCTTGTC GCGATCCATC CTGACTTCAG GATGCTGGCT 
CTGGCCAACA GGCCTGGCTT CCCCTTCTTG GGCAATGATT TCTTTGGAAC TTTAGGTGAC 
ATTTTTAGCT GTCATGCCAT TGACAACCCC AAGCCCCACT CAGAGCTTTC GATGCTCAAA 
CAGTACGGCC CAGACGTGCC TGAGCCCGTC CTGCAGAAGC TCGTGGCAGC CTTTGGAGAG 
CTGAGGAACT TGGCTGACCA AGGGATTATC AACTACCCTT ATTCCACCAG AGAAGTGGTC 
AACATCGTCA AGCACTTACA GAAATTTCCC ACTGAAGGCC TCTCCAGTGT GGTTCGGAAT 
GTATTTGACT TCGATTCTTA CAACAACGAC ATGAGGGAGA TTTTGATGAA CACTTTGCAC 
AAATATGGGA TCCCCATTGG AGCAAAGCCT ACCAACGTGC AGCTGGCAAA GGAGTTTCCT 
CTGCCAGAGA AGACATTCAT GGGCTACTGG ATAGTTGGCC AGACGGGAAA TGGAATGCAG 
AAGGTCTTGT GTCCAGCTGA AACAAATCAC GTTGACATAA AGGGCCCAGT ACTTGTGAAC 
ATGGAAAAGT ACCCAATAGA AAAACATGAA GCGAGATTCC TAAGCTTTAC TGAAGAGTGT 
ACGTCCTGGA AGTTTCCATT GGATGAAGTC AATTTAATCT GTGACATCGC TGTATCACAT 
GTCATAGCAC AAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011243340.1
CDS	225..3602
Translation	MQSRLLLLGAPGGLGDVASRRVRLLLRQVLRGRPGGDQQRLEVRLLHSGATDSGETVSIGDVSYKLKTPKNPELVPQNYISDSPAQSIVQHLRWLMQKDLLGQDVFLIGPPGPLRRSVAMQYLELTKREVEYIALSRDTTETDLKQRREIRAGTAFYIDQCAVRAATEGRTLVLEGLEKAERNVLPVLNNLLENREMQLEDGRFLMSAERYDKLLQDHTKEELDAWKIVRVSENFRVIALGLPVPRYSGNPLDPPLRSRFQARDIYFLPFQDQLKLLYSVGANVSAEKISQLLSFATTLCSQESSTLGLPDFPLDSLPEAVQILDSFPMMSIEHALQWVYPYTLLLGHEGKMAVEGVLKRFELQGSGHSLLPKEIVRVERMTDSHGSYAHVTIRVAGKEVTIKVPAGTRAVNQPCAPDHFIQTVSHKQLLAEMVQSHMVKDICLIGGKGCGKTVIAKNFAALLGYSIEPIMLYQDMTARDLLQQRYTLPNGDTAWRSSPLVSAAREGKLVLLDGIHRVNAGTLAVLQRLIHDRELSLYDGSRLLREDRYLSLKEKLQLTDEQLQNRSIFPIHPSFRIIALAEPPIVGSTTQQWLGPEFLTMFFFHHMKPLVKSEEIQVIKETVPNVPQEALEKLLSVTHKLRETQDPTAQSLAASLSTRQLLRISRRLSKYPSENLHDAITKACLSRFLPSLAQSALEKNLADAAIETNTEDSLEPELENYKCEVVAGSLKIGAVSVPVHNAHEKMKVPDVLFYDNVQHMVVMEDMLKDFVLGEHLLLVGNQGVGKNKIVDRFLHLLNRPREYIQLHRDTTVQSLTLQPTVKGGLIVYEDSPLVKAVKLGHILVVDEADKAPTNVTCILKTLVENGEMILADGRRIVADAANVDGRENLVAIHPDFRMLALANRPGFPFLGNDFFGTLGDIFSCHAIDNPKPHSELSMLKQYGPDVPEPVLQKLVAAFGELRNLADQGIINYPYSTREVVNIVKHLQKFPTEGLSSVVRNVFDFDSYNNDMREILMNTLHKYGIPIGAKPTNVQLAKEFPLPEKTFMGYWIVGQTGNGMQKVLCPAETNHVDIKGPVLVNMEKYPIEKHEARFLSFTEECTSWKFPLDEVNLICDIAVSHVIAQS

Target ORF information:

RefSeq Version	XM_011245038.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus von Willebrand factor A domain containing 8 (Vwa8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245038.3

ORF Insert Sequence:

ATGCAGTCAC GCCTCCTCCT CCTAGGAGCC CCGGGCGGCC TTGGGGACGT GGCCTCCCGG 
CGAGTGCGGC TGCTCTTGCG GCAGGTGTTG CGGGGCAGGC CGGGCGGGGA CCAGCAGCGG 
CTGGAGGTCA GGCTGCTGCA CTCTGGGGCG ACCGACTCAG GTGAAACGGT TAGTATTGGA 
GATGTGTCCT ACAAGCTGAA AACTCCCAAG AATCCAGAAC TTGTCCCACA GAACTACATT 
TCAGACTCTC CAGCTCAGTC CATAGTCCAG CATCTGAGAT GGCTGATGCA GAAGGATCTG 
CTGGGGCAGG ATGTCTTTCT CATCGGACCT CCTGGGCCTC TCCGGCGCTC GGTGGCTATG 
CAGTACTTGG AGCTGACCAA ACGAGAGGTG GAATACATCG CCCTGTCAAG GGACACCACT 
GAAACTGACC TCAAACAGCG CCGGGAGATC CGAGCTGGCA CAGCCTTTTA CATTGACCAG 
TGTGCGGTTC GGGCTGCCAC AGAAGGCAGG ACGCTGGTTT TAGAAGGCTT GGAAAAGGCG 
GAGAGAAACG TGCTTCCAGT ACTGAACAAC TTGCTGGAGA ACCGGGAGAT GCAGCTTGAA 
GATGGGCGCT TCCTCATGTC TGCGGAGCGC TATGACAAGC TTCTCCAAGA TCACACTAAA 
GAGGAGCTGG ACGCGTGGAA GATTGTCCGA GTCAGTGAGA ATTTCCGAGT GATTGCCTTG 
GGCTTGCCAG TGCCCAGGTA CTCTGGGAAT CCATTAGACC CCCCTCTCCG GTCTCGATTT 
CAAGCCAGAG ATATCTATTT TCTACCCTTT CAGGACCAAC TAAAACTATT GTATTCAGTT 
GGAGCCAATG TTTCTGCTGA GAAGATTTCT CAGCTCTTGT CCTTTGCGAC AACCCTCTGC 
TCCCAAGAAT CCTCTACGCT TGGGCTTCCA GACTTTCCCT TAGATAGTTT GCCAGAGGCA 
GTTCAAATCC TGGACTCCTT TCCCATGATG TCGATTGAAC ATGCACTCCA GTGGGTGTAT 
CCTTACACTC TTTTACTCGG ACACGAGGGG AAGATGGCCG TGGAGGGTGT TTTAAAACGC 
TTTGAGCTCC AAGGTTCTGG ACATTCCCTG CTTCCTAAGG AGATTGTAAG AGTGGAGAGG 
ATGACTGACA GCCACGGCTC CTATGCCCAC GTGACAATCC GGGTCGCAGG AAAAGAGGTG 
ACCATTAAGG TACCAGCGGG GACCAGAGCA GTAAACCAGC CTTGTGCTCC TGACCATTTC 
ATTCAGACTG TCAGTCACAA GCAATTACTG GCTGAGATGG TGCAGTCTCA TATGGTGAAG 
GACATATGCT TAATTGGAGG GAAGGGCTGT GGGAAAACGG TCATCGCTAA GAACTTTGCT 
GCTCTCCTAG GATACAGCAT AGAACCCATC ATGCTTTACC AGGATATGAC TGCGCGGGAC 
CTGCTACAGC AGCGGTACAC GCTCCCCAAC GGCGACACCG CCTGGCGCTC CTCACCCCTT 
GTGAGTGCTG CCCGGGAAGG CAAACTGGTC CTGTTGGATG GTATCCACCG CGTCAACGCT 
GGCACACTGG CTGTATTGCA AAGACTGATC CATGACCGGG AGCTCAGCCT GTATGATGGA 
TCTCGGCTGC TGAGGGAGGA CAGGTACCTG AGCTTGAAGG AGAAGCTACA ACTGACCGAT 
GAGCAGCTAC AGAACAGGTC CATTTTTCCT ATCCACCCTT CCTTCAGAAT CATTGCGTTG 
GCAGAGCCCC CAATTGTTGG AAGCACAACA CAGCAGTGGC TGGGACCAGA ATTTTTAACT 
ATGTTCTTTT TCCATCACAT GAAGCCTCTT GTGAAAAGTG AAGAAATCCA AGTGATAAAG 
GAGACGGTTC CAAATGTACC TCAGGAAGCA CTGGAGAAAT TGCTGTCAGT CACTCACAAA 
CTCAGGGAGA CCCAGGATCC CACAGCACAG TCCTTGGCAG CCTCGCTTTC CACCCGTCAG 
CTCTTACGGA TCTCTCGCCG GCTCTCGAAG TATCCCAGCG AAAATCTTCA TGATGCCATT 
ACCAAAGCCT GTCTTTCCAG GTTCTTACCA AGCCTTGCTC AGTCTGCACT GGAGAAGAAT 
CTGGCAGATG CGGCAATAGA AACAAACACT GAGGACAGCC TGGAGCCAGA GCTGGAGAAT 
TACAAGTGTG AAGTAGTCGC CGGGTCTCTG AAGATTGGCG CCGTCAGTGT GCCAGTGCAC 
AATGCCCATG AGAAGATGAA AGTGCCTGAC GTTCTTTTCT ACGACAATGT TCAGCACATG 
GTAGTGATGG AAGACATGCT TAAAGACTTT GTCCTTGGCG AACACTTACT ATTGGTTGGT 
AACCAGGGGG TAGGAAAGAA CAAGATTGTG GACCGATTCC TTCACCTGCT CAACAGACCC 
CGAGAATATA TACAGCTGCA CAGGGACACC ACAGTGCAGT CTCTGACTCT CCAGCCCACA 
GTGAAAGGTG GACTGATTGT ATATGAAGAC TCACCTCTGG TTAAAGCAGT AAAGTTGGGT 
CATATTCTAG TAGTAGATGA GGCAGACAAA GCCCCAACAA ATGTCACCTG TATTTTGAAG 
ACTCTGGTAG AAAACGGAGA AATGATTCTT GCGGACGGAA GACGTATTGT TGCAGATGCA 
GCAAATGTGG ATGGAAGAGA GAACCTTGTC GCGATCCATC CTGACTTCAG GATGCTGGCT 
CTGGCCAACA GGCCTGGCTT CCCCTTCTTG GGCAATGATT TCTTTGGAAC TTTAGGTGAC 
ATTTTTAGCT GTCATGCCAT TGACAACCCC AAGCCCCACT CAGAGCTTTC GATGCTCAAA 
CAGTACGGCC CAGACGTGCC TGAGCCCGTC CTGCAGAAGC TCGTGGCAGC CTTTGGAGAG 
CTGAGGAACT TGGCTGACCA AGGGATTATC AACTACCCTT ATTCCACCAG AGAAGTGGTC 
AACATCGTCA AGCACTTACA GAAATTTCCC ACTGAAGGCC TCTCCAGTGT GGTTCGGAAT 
GTATTTGACT TCGATTCTTA CAACAACGAC ATGAGGGAGA TTTTGATGAA CACTTTGCAC 
AAATATGGGA TCCCCATTGG AGCAAAGCCT ACCAACGTGC AGCTGGCAAA GGAGTTTCCT 
CTGCCAGAGA AGACATTCAT GGGCTACTGG ATAGTTGGCC AGACGGGAAA TGGAATGCAG 
AAGGTCTTGT GTCCAGCTGA AACAAATCAC GTTGACATAA AGGGCCCAGT ACTTGTGAAC 
ATGGAAAAGT ACCCAATAGA AAAACATGAA GCGAGATTCC TAAGCTTTAC TGAAGAGTGT 
ACGTCCTGGA AGTTTCCATT GGATGAAGTC AATTTAATCT GTGACATCGC TGTATCACAT 
GTCATAGCAC AAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vwa8 ( XM_017315982.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171471.1 Mus musculus von Willebrand factor A domain containing 8 (Vwa8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu78740
Clone ID Related Accession (Same CDS sequence)	XM_017315982.1
Accession Version	XM_017315982.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4293bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	von Willebrand factor A domain-containing protein 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGCGC GGGACCTGCT ACAGCAGCGG TACACGCTCC CCAACGGCGA CACCGCCTGG 
CGCTCCTCAC CCCTTGTGAG TGCTGCCCGG GAAGGCAAAC TGGTCCTGTT GGATGGTATC 
CACCGCGTCA ACGCTGGCAC ACTGGCTGTA TTGCAAAGAC TGATCCATGA CCGGGAGCTC 
AGCCTGTATG ATGGATCTCG GCTGCTGAGG GAGGACAGGT ACCTGAGCTT GAAGGAGAAG 
CTACAACTGA CCGATGAGCA GCTACAGAAC AGGTCCATTT TTCCTATCCA CCCTTCCTTC 
AGAATCATTG CGTTGGCAGA GCCCCCAATT GTTGGAAGCA CAACACAGCA GTGGCTGGGA 
CCAGAATTTT TAACTATGTT CTTTTTCCAT CACATGAAGC CTCTTGTGAA AAGTGAAGAA 
ATCCAAGTGA TAAAGGAGAC GGTTCCAAAT GTACCTCAGG AAGCACTGGA GAAATTGCTG 
TCAGTCACTC ACAAACTCAG GGAGACCCAG GATCCCACAG CACAGTCCTT GGCAGCCTCG 
CTTTCCACCC GTCAGCTCTT ACGGATCTCT CGCCGGCTCT CGAAGTATCC CAGCGAAAAT 
CTTCATGATG CCATTACCAA AGCCTGTCTT TCCAGGTTCT TACCAAGCCT TGCTCAGTCT 
GCACTGGAGA AGAATCTGGC AGATGCGGCA ATAGAAACAA ACACTGAGGA CAGCCTGGAG 
CCAGAGCTGG AGAATTACAA GTGTGAAGTA GTCGCCGGGT CTCTGAAGAT TGGCGCCGTC 
AGTGTGCCAG TGCACAATGC CCATGAGAAG ATGAAAGTGC CTGACGTTCT TTTCTACGAC 
AATGTTCAGC ACATGGTAGT GATGGAAGAC ATGCTTAAAG ACTTTGTCCT TGGCGAACAC 
TTACTATTGG TTGGTAACCA GGGGGTAGGA AAGAACAAGA TTGTGGACCG ATTCCTTCAC 
CTGCTCAACA GACCCCGAGA ATATATACAG CTGCACAGGG ACACCACAGT GCAGTCTCTG 
ACTCTCCAGC CCACAGTGAA AGGTGGACTG ATTGTATATG AAGACTCACC TCTGGTTAAA 
GCAGTAAAGT TGGGTCATAT TCTAGTAGTA GATGAGGCAG ACAAAGCCCC AACAAATGTC 
ACCTGTATTT TGAAGACTCT GGTAGAAAAC GGAGAAATGA TTCTTGCGGA CGGAAGACGT 
ATTGTTGCAG ATGCAGCAAA TGTGGATGGA AGAGAGAACC TTGTCGCGAT CCATCCTGAC 
TTCAGGATGC TGGCTCTGGC CAACAGGCCT GGCTTCCCCT TCTTGGGCAA TGATTTCTTT 
GGAACTTTAG GTGACATTTT TAGCTGTCAT GCCATTGACA ACCCCAAGCC CCACTCAGAG 
CTTTCGATGC TCAAACAGTA CGGCCCAGAC GTGCCTGAGC CCGTCCTGCA GAAGCTCGTG 
GCAGCCTTTG GAGAGCTGAG GAACTTGGCT GACCAAGGGA TTATCAACTA CCCTTATTCC 
ACCAGAGAAG TGGTCAACAT CGTCAAGCAC TTACAGAAAT TTCCCACTGA AGGCCTCTCC 
AGTGTGGTTC GGAATGTATT TGACTTCGAT TCTTACAACA ACGACATGAG GGAGATTTTG 
ATGAACACTT TGCACAAATA TGGGATCCCC ATTGGAGCAA AGCCTACCAA CGTGCAGCTG 
GCAAAGGAGT TTCCTCTGCC AGAGAAGACA TTCATGGGCT ACTGGATAGT TGGCCAGACG 
GGAAATGGAA TGCAGAAGGT CTTGTGTCCA GCTGAAACAA ATCACGTTGA CATAAAGGGC 
CCAGTACTTG TGAACATGGA AAAGTACCCA ATAGAAAAAC ATGAAGCGAG ATTCCTAAGC 
TTTACTGAAG AGTGTACGTC CTGGAAGTTT CCATTGGATG AAGTCAATTT AATCTGTGAC 
ATCGCTGTAT CACATGAAAA CGGAGAACAG ACTCTGTATG TGGCTGCGTG CAACCCGGTT 
TCCTTGTACT TCATGAATAT GACAGGGAAA AATGGCTTCT TTGTGGACTT CTTCGACATC 
TTCCCAAGGA TGGCCAGTGG GTCCTGGCGA CCATTTGTAA CAGTAGCCCC ACTGGGAAGT 
CCTCTGAGGG GTCAAGTTGT TCTGCATGAG GAGCAGAGTA ATGCCGTCCT CTTACTCGAC 
ACTACTGGCA GTGCCATTCG CCGTCTCGTC CTTCCCACAG AAGAGTTTAC ATCTAAGAAG 
TCTTCATGGT GGAGCAAGGA GGAAGGTGAA ACTTATAGAA TGTGTAAAGA ATTCTCACAC 
AAAAACTGGG TAGTATTCTA CAAACAAACA GGGAATAGCT TGACTGTCCT GGATGTCTTA 
GAAGGACTAG CTCACACCAT CTCACTTCCC ATCAACCTCA GGACAGTTTT CCTGGTGGCG 
GAGGACAAAT GGCTTCTTGT GGAAAACGAA ACAAATCAGA AGTATCTTTT GACTAAGCCT 
GCACACATTG GTTCTGAGGA CACTGGGGCC TGTCAGTTGT ATATGTTGAA AGAAGAGCTG 
CCTAGCACAG GATTTGGAGT CACACAAGAA ACAGAGTTCT GCATACCTGA TAAAGTGTCA 
AGTGATCAGC TGTCATCTGA AAACCTAACT TCAGCTGTGG GCCAGAAGAT CGCCTCTCCT 
AACCGTATTC TCTCGGATGA AAATAGCCAC GCTACCATAG TTGTTGGCTT CCCGGACCTC 
ATGTCACCCA GTGAAGTTTA TTCATGGAAG AGGTCTTCGT CTTTGAGACA ACCAAGTGTT 
ACCAGCATGA CAATGTACAC AGGGAAGCGG ACAAATGCCA CCCCACGGCA CAACAACTGC 
GTGACTCTCA CACATACTAA TCAGGTGGTG AGGATTCTGC CCCCAGGAGA AGTCCCTCTG 
AAAGACCTCT ACCCAAAAGA TGTTACTCCC CCCCAGACAG CTGGCTACAT AGAGGTTACT 
GATCTCCAAG CAAAGAAACT CCGTTACATC CCTGTCCCCA GCGCAGAGTC TCTCTCGCCA 
TACACCGCAT GGATATCTGC TATTTCAGAC ACAGATGCTC TGCTGGCTGA GTGGGACAAG 
AGCAGCGTCG TGACTGTTGA TATGGGAGGG CGTGTCAGGC TCTGGGAAAC TGGACTTGAA 
CGTCTGCAGC AGTCGCTCAT GGAATGGAGA AACATGATTG GACAAGACAG TGACAAACAT 
GTGCAGATAA CAATCGAAAG AGACAGTAAT GAAGACGTGA GTGACCCCAA ACATGGCAAG 
GAGGACCCAG ACAACATGCC CCATGTGGGT GGCAATACCT GGGCTGGTGG AACAGGGGGA 
AGAGACACCG CAGGTCTTGG TGGCAAAGGA GGTCCTTATC GCTTGGATGC AGGCCACCCA 
GTGTATCAAG TCTCTGAGGT GGAGAAAGAT GCAGTTCCTG AGGATGTCAA GAGAGCTGCA 
AGAGAGATGG CCCAGAAAGC ATTTCAGCAG AGACTAAAGG AGATCCAGAT GAGCGAGTAC 
GACGCCGCAA CCTACGAGCG GTTTTCAAGT GCTGTCCAGC GGCAGGTGCA TGCTCTCCGC 
ATCATCCTGG ATAATCTACA GGCTAAAGGC AAAGAAAGAC AGTGGCTAAG ACACCAAGCT 
ACCGGAGAAC TGGACGACGC CAAGATCATT GACGGGCTGG CGGGAGAAAA GTCCATCTAC 
AAACGTCGGG GTGACCTGGA ACCCCAGCTG GGTAGTCCAC AACAGAAGCC CAAGCGCCTG 
CGCCTGGTGG TGGACGTCTC CGGCAGCATG TACCGCTTCA ACGGGGTGGA TCGCCGGCTA 
GAGCGCTCGA TGGAGGCAGT GTGCATGGTC ATGGAAGCCT TCGAGAACTA CGAGGAGAAA 
TTCAAGTACG ACATCGCTGG ACACTCGGGA GATGGCTACA ACATTAAGCT CGTTCCAGTT 
AACCAAATCC CCAAGAACAA TAAGCAGAGA CTAGAAATTC TGAAGACGAT GCACGAGCAC 
TCTCAGTTCT GCATGAGTGG GGACCACACA CTTGAGGGAA CGGAACACGC CATCAAGGAC 
ATTACCACAG AAGAAGCTGA TGAGTACTTT GTCATCATCT TAAGTGATGC AAATCTGTCC 
CGATACGGAA TAAACCCGGC CCGCTTTGCC CAGATCCTGA CAAGCGATCC TCAAGTAAAC 
GCCTTTGCCA TTTTTATCGG CTCTTTGGGG GATCAGGCAG CCAGGCTTCA GAGAACTCTG 
CCAGCTGGCC GGTCCTTCAT CGCTATGGAT ACCAAGAAGA TCCCTCAGAT CTTACAACAG 
ATCTTCACCT CCACCATGCT GTCCAGTATC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171471.1
CDS	87..4379
Translation	MTARDLLQQRYTLPNGDTAWRSSPLVSAAREGKLVLLDGIHRVNAGTLAVLQRLIHDRELSLYDGSRLLREDRYLSLKEKLQLTDEQLQNRSIFPIHPSFRIIALAEPPIVGSTTQQWLGPEFLTMFFFHHMKPLVKSEEIQVIKETVPNVPQEALEKLLSVTHKLRETQDPTAQSLAASLSTRQLLRISRRLSKYPSENLHDAITKACLSRFLPSLAQSALEKNLADAAIETNTEDSLEPELENYKCEVVAGSLKIGAVSVPVHNAHEKMKVPDVLFYDNVQHMVVMEDMLKDFVLGEHLLLVGNQGVGKNKIVDRFLHLLNRPREYIQLHRDTTVQSLTLQPTVKGGLIVYEDSPLVKAVKLGHILVVDEADKAPTNVTCILKTLVENGEMILADGRRIVADAANVDGRENLVAIHPDFRMLALANRPGFPFLGNDFFGTLGDIFSCHAIDNPKPHSELSMLKQYGPDVPEPVLQKLVAAFGELRNLADQGIINYPYSTREVVNIVKHLQKFPTEGLSSVVRNVFDFDSYNNDMREILMNTLHKYGIPIGAKPTNVQLAKEFPLPEKTFMGYWIVGQTGNGMQKVLCPAETNHVDIKGPVLVNMEKYPIEKHEARFLSFTEECTSWKFPLDEVNLICDIAVSHENGEQTLYVAACNPVSLYFMNMTGKNGFFVDFFDIFPRMASGSWRPFVTVAPLGSPLRGQVVLHEEQSNAVLLLDTTGSAIRRLVLPTEEFTSKKSSWWSKEEGETYRMCKEFSHKNWVVFYKQTGNSLTVLDVLEGLAHTISLPINLRTVFLVAEDKWLLVENETNQKYLLTKPAHIGSEDTGACQLYMLKEELPSTGFGVTQETEFCIPDKVSSDQLSSENLTSAVGQKIASPNRILSDENSHATIVVGFPDLMSPSEVYSWKRSSSLRQPSVTSMTMYTGKRTNATPRHNNCVTLTHTNQVVRILPPGEVPLKDLYPKDVTPPQTAGYIEVTDLQAKKLRYIPVPSAESLSPYTAWISAISDTDALLAEWDKSSVVTVDMGGRVRLWETGLERLQQSLMEWRNMIGQDSDKHVQITIERDSNEDVSDPKHGKEDPDNMPHVGGNTWAGGTGGRDTAGLGGKGGPYRLDAGHPVYQVSEVEKDAVPEDVKRAAREMAQKAFQQRLKEIQMSEYDAATYERFSSAVQRQVHALRIILDNLQAKGKERQWLRHQATGELDDAKIIDGLAGEKSIYKRRGDLEPQLGSPQQKPKRLRLVVDVSGSMYRFNGVDRRLERSMEAVCMVMEAFENYEEKFKYDIAGHSGDGYNIKLVPVNQIPKNNKQRLEILKTMHEHSQFCMSGDHTLEGTEHAIKDITTEEADEYFVIILSDANLSRYGINPARFAQILTSDPQVNAFAIFIGSLGDQAARLQRTLPAGRSFIAMDTKKIPQILQQIFTSTMLSSI

Target ORF information:

RefSeq Version	XM_017315982.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus von Willebrand factor A domain containing 8 (Vwa8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315982.1

ORF Insert Sequence:

ATGACTGCGC GGGACCTGCT ACAGCAGCGG TACACGCTCC CCAACGGCGA CACCGCCTGG 
CGCTCCTCAC CCCTTGTGAG TGCTGCCCGG GAAGGCAAAC TGGTCCTGTT GGATGGTATC 
CACCGCGTCA ACGCTGGCAC ACTGGCTGTA TTGCAAAGAC TGATCCATGA CCGGGAGCTC 
AGCCTGTATG ATGGATCTCG GCTGCTGAGG GAGGACAGGT ACCTGAGCTT GAAGGAGAAG 
CTACAACTGA CCGATGAGCA GCTACAGAAC AGGTCCATTT TTCCTATCCA CCCTTCCTTC 
AGAATCATTG CGTTGGCAGA GCCCCCAATT GTTGGAAGCA CAACACAGCA GTGGCTGGGA 
CCAGAATTTT TAACTATGTT CTTTTTCCAT CACATGAAGC CTCTTGTGAA AAGTGAAGAA 
ATCCAAGTGA TAAAGGAGAC GGTTCCAAAT GTACCTCAGG AAGCACTGGA GAAATTGCTG 
TCAGTCACTC ACAAACTCAG GGAGACCCAG GATCCCACAG CACAGTCCTT GGCAGCCTCG 
CTTTCCACCC GTCAGCTCTT ACGGATCTCT CGCCGGCTCT CGAAGTATCC CAGCGAAAAT 
CTTCATGATG CCATTACCAA AGCCTGTCTT TCCAGGTTCT TACCAAGCCT TGCTCAGTCT 
GCACTGGAGA AGAATCTGGC AGATGCGGCA ATAGAAACAA ACACTGAGGA CAGCCTGGAG 
CCAGAGCTGG AGAATTACAA GTGTGAAGTA GTCGCCGGGT CTCTGAAGAT TGGCGCCGTC 
AGTGTGCCAG TGCACAATGC CCATGAGAAG ATGAAAGTGC CTGACGTTCT TTTCTACGAC 
AATGTTCAGC ACATGGTAGT GATGGAAGAC ATGCTTAAAG ACTTTGTCCT TGGCGAACAC 
TTACTATTGG TTGGTAACCA GGGGGTAGGA AAGAACAAGA TTGTGGACCG ATTCCTTCAC 
CTGCTCAACA GACCCCGAGA ATATATACAG CTGCACAGGG ACACCACAGT GCAGTCTCTG 
ACTCTCCAGC CCACAGTGAA AGGTGGACTG ATTGTATATG AAGACTCACC TCTGGTTAAA 
GCAGTAAAGT TGGGTCATAT TCTAGTAGTA GATGAGGCAG ACAAAGCCCC AACAAATGTC 
ACCTGTATTT TGAAGACTCT GGTAGAAAAC GGAGAAATGA TTCTTGCGGA CGGAAGACGT 
ATTGTTGCAG ATGCAGCAAA TGTGGATGGA AGAGAGAACC TTGTCGCGAT CCATCCTGAC 
TTCAGGATGC TGGCTCTGGC CAACAGGCCT GGCTTCCCCT TCTTGGGCAA TGATTTCTTT 
GGAACTTTAG GTGACATTTT TAGCTGTCAT GCCATTGACA ACCCCAAGCC CCACTCAGAG 
CTTTCGATGC TCAAACAGTA CGGCCCAGAC GTGCCTGAGC CCGTCCTGCA GAAGCTCGTG 
GCAGCCTTTG GAGAGCTGAG GAACTTGGCT GACCAAGGGA TTATCAACTA CCCTTATTCC 
ACCAGAGAAG TGGTCAACAT CGTCAAGCAC TTACAGAAAT TTCCCACTGA AGGCCTCTCC 
AGTGTGGTTC GGAATGTATT TGACTTCGAT TCTTACAACA ACGACATGAG GGAGATTTTG 
ATGAACACTT TGCACAAATA TGGGATCCCC ATTGGAGCAA AGCCTACCAA CGTGCAGCTG 
GCAAAGGAGT TTCCTCTGCC AGAGAAGACA TTCATGGGCT ACTGGATAGT TGGCCAGACG 
GGAAATGGAA TGCAGAAGGT CTTGTGTCCA GCTGAAACAA ATCACGTTGA CATAAAGGGC 
CCAGTACTTG TGAACATGGA AAAGTACCCA ATAGAAAAAC ATGAAGCGAG ATTCCTAAGC 
TTTACTGAAG AGTGTACGTC CTGGAAGTTT CCATTGGATG AAGTCAATTT AATCTGTGAC 
ATCGCTGTAT CACATGAAAA CGGAGAACAG ACTCTGTATG TGGCTGCGTG CAACCCGGTT 
TCCTTGTACT TCATGAATAT GACAGGGAAA AATGGCTTCT TTGTGGACTT CTTCGACATC 
TTCCCAAGGA TGGCCAGTGG GTCCTGGCGA CCATTTGTAA CAGTAGCCCC ACTGGGAAGT 
CCTCTGAGGG GTCAAGTTGT TCTGCATGAG GAGCAGAGTA ATGCCGTCCT CTTACTCGAC 
ACTACTGGCA GTGCCATTCG CCGTCTCGTC CTTCCCACAG AAGAGTTTAC ATCTAAGAAG 
TCTTCATGGT GGAGCAAGGA GGAAGGTGAA ACTTATAGAA TGTGTAAAGA ATTCTCACAC 
AAAAACTGGG TAGTATTCTA CAAACAAACA GGGAATAGCT TGACTGTCCT GGATGTCTTA 
GAAGGACTAG CTCACACCAT CTCACTTCCC ATCAACCTCA GGACAGTTTT CCTGGTGGCG 
GAGGACAAAT GGCTTCTTGT GGAAAACGAA ACAAATCAGA AGTATCTTTT GACTAAGCCT 
GCACACATTG GTTCTGAGGA CACTGGGGCC TGTCAGTTGT ATATGTTGAA AGAAGAGCTG 
CCTAGCACAG GATTTGGAGT CACACAAGAA ACAGAGTTCT GCATACCTGA TAAAGTGTCA 
AGTGATCAGC TGTCATCTGA AAACCTAACT TCAGCTGTGG GCCAGAAGAT CGCCTCTCCT 
AACCGTATTC TCTCGGATGA AAATAGCCAC GCTACCATAG TTGTTGGCTT CCCGGACCTC 
ATGTCACCCA GTGAAGTTTA TTCATGGAAG AGGTCTTCGT CTTTGAGACA ACCAAGTGTT 
ACCAGCATGA CAATGTACAC AGGGAAGCGG ACAAATGCCA CCCCACGGCA CAACAACTGC 
GTGACTCTCA CACATACTAA TCAGGTGGTG AGGATTCTGC CCCCAGGAGA AGTCCCTCTG 
AAAGACCTCT ACCCAAAAGA TGTTACTCCC CCCCAGACAG CTGGCTACAT AGAGGTTACT 
GATCTCCAAG CAAAGAAACT CCGTTACATC CCTGTCCCCA GCGCAGAGTC TCTCTCGCCA 
TACACCGCAT GGATATCTGC TATTTCAGAC ACAGATGCTC TGCTGGCTGA GTGGGACAAG 
AGCAGCGTCG TGACTGTTGA TATGGGAGGG CGTGTCAGGC TCTGGGAAAC TGGACTTGAA 
CGTCTGCAGC AGTCGCTCAT GGAATGGAGA AACATGATTG GACAAGACAG TGACAAACAT 
GTGCAGATAA CAATCGAAAG AGACAGTAAT GAAGACGTGA GTGACCCCAA ACATGGCAAG 
GAGGACCCAG ACAACATGCC CCATGTGGGT GGCAATACCT GGGCTGGTGG AACAGGGGGA 
AGAGACACCG CAGGTCTTGG TGGCAAAGGA GGTCCTTATC GCTTGGATGC AGGCCACCCA 
GTGTATCAAG TCTCTGAGGT GGAGAAAGAT GCAGTTCCTG AGGATGTCAA GAGAGCTGCA 
AGAGAGATGG CCCAGAAAGC ATTTCAGCAG AGACTAAAGG AGATCCAGAT GAGCGAGTAC 
GACGCCGCAA CCTACGAGCG GTTTTCAAGT GCTGTCCAGC GGCAGGTGCA TGCTCTCCGC 
ATCATCCTGG ATAATCTACA GGCTAAAGGC AAAGAAAGAC AGTGGCTAAG ACACCAAGCT 
ACCGGAGAAC TGGACGACGC CAAGATCATT GACGGGCTGG CGGGAGAAAA GTCCATCTAC 
AAACGTCGGG GTGACCTGGA ACCCCAGCTG GGTAGTCCAC AACAGAAGCC CAAGCGCCTG 
CGCCTGGTGG TGGACGTCTC CGGCAGCATG TACCGCTTCA ACGGGGTGGA TCGCCGGCTA 
GAGCGCTCGA TGGAGGCAGT GTGCATGGTC ATGGAAGCCT TCGAGAACTA CGAGGAGAAA 
TTCAAGTACG ACATCGCTGG ACACTCGGGA GATGGCTACA ACATTAAGCT CGTTCCAGTT 
AACCAAATCC CCAAGAACAA TAAGCAGAGA CTAGAAATTC TGAAGACGAT GCACGAGCAC 
TCTCAGTTCT GCATGAGTGG GGACCACACA CTTGAGGGAA CGGAACACGC CATCAAGGAC 
ATTACCACAG AAGAAGCTGA TGAGTACTTT GTCATCATCT TAAGTGATGC AAATCTGTCC 
CGATACGGAA TAAACCCGGC CCGCTTTGCC CAGATCCTGA CAAGCGATCC TCAAGTAAAC 
GCCTTTGCCA TTTTTATCGG CTCTTTGGGG GATCAGGCAG CCAGGCTTCA GAGAACTCTG 
CCAGCTGGCC GGTCCTTCAT CGCTATGGAT ACCAAGAAGA TCCCTCAGAT CTTACAACAG 
ATCTTCACCT CCACCATGCT GTCCAGTATC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vwa8 ( NM_173758.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_776119.2 Mus musculus von Willebrand factor A domain containing 8 (Vwa8), transcript variant 2, mRNA.

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CloneID	OMu03757
Clone ID Related Accession (Same CDS sequence)	NM_173758.3
Accession Version	NM_173758.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3117bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Mus musculus(house mouse)
Product	von Willebrand factor A domain-containing protein 8 isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC155167.4 and AC124709.3. On Apr 18, 2009 this sequence version replaced NM_173758.2. Transcript Variant: This variant (2) represents a shorter transcript with a distinct UTR and polyA signal and site, compared to variant 1. The resulting predicted protein (isoform 2) is much shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK154108.1, AK033642.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGTCAC GCCTCCTCCT CCTAGGAGCC CCGGGCGGCC TTGGGGACGT GGCCTCCCGG 
CGAGTGCGGC TGCTCTTGCG GCAGGTGTTG CGGGGCAGGC CGGGCGGGGA CCAGCAGCGG 
CTGGAGGTCA GGCTGCTGCA CTCTGGGGCG ACCGACTCAG GTGAAACGGT TAGTATTGGA 
GATGTGTCCT ACAAGCTGAA AACTCCCAAG AATCCAGAAC TTGTCCCACA GAACTACATT 
TCAGACTCTC CAGCTCAGTC CATAGTCCAG CATCTGAGAT GGCTGATGCA GAAGGATCTG 
CTGGGGCAGG ATGTCTTTCT CATCGGACCT CCTGGGCCTC TCCGGCGCTC GGTGGCTATG 
CAGTACTTGG AGCTGACCAA ACGAGAGGTG GAATACATCG CCCTGTCAAG GGACACCACT 
GAAACTGACC TCAAACAGCG CCGGGAGATC CGAGCTGGCA CAGCCTTTTA CATTGACCAG 
TGTGCGGTTC GGGCTGCCAC AGAAGGCAGG ACGCTGGTTT TAGAAGGCTT GGAAAAGGCG 
GAGAGAAACG TGCTTCCAGT ACTGAACAAC TTGCTGGAGA ACCGGGAGAT GCAGCTTGAA 
GATGGGCGCT TCCTCATGTC TGCGGAGCGC TATGACAAGC TTCTCCAAGA TCACACTAAA 
GAGGAGCTGG ACGCGTGGAA GATTGTCCGA GTCAGTGAGA ATTTCCGAGT GATTGCCTTG 
GGCTTGCCAG TGCCCAGGTA CTCTGGGAAT CCATTAGACC CCCCTCTCCG GTCTCGATTT 
CAAGCCAGAG ATATCTATTT TCTACCCTTT CAGGACCAAC TAAAACTATT GTATTCAGTT 
GGAGCCAATG TTTCTGCTGA GAAGATTTCT CAGCTCTTGT CCTTTGCGAC AACCCTCTGC 
TCCCAAGAAT CCTCTACGCT TGGGCTTCCA GACTTTCCCT TAGATAGTTT GCCAGAGGCA 
GTTCAAATCC TGGACTCCTT TCCCATGATG TCGATTGAAC ATGCACTCCA GTGGGTGTAT 
CCTTACACTC TTTTACTCGG ACACGAGGGG AAGATGGCCG TGGAGGGTGT TTTAAAACGC 
TTTGAGCTCC AAGGTTCTGG ACATTCCCTG CTTCCTAAGG AGATTGTAAG AGTGGAGAGG 
ATGACTGACA GCCACGGCTC CTATGCCCAC GTGACAATCC GGGTCGCAGG AAAAGAGGTG 
ACCATTAAGG TACCAGCGGG GACCAGAGCA GTAAACCAGC CTTGTGCTCC TGACCATTTC 
ATTCAGACTG TCAGTCACAA GCAATTACTG GCTGAGATGG TGCAGTCTCA TATGGTGAAG 
GACATATGCT TAATTGGAGG GAAGGGCTGT GGGAAAACGG TCATCGCTAA GAACTTTGCT 
GCTCTCCTAG GATACAGCAT AGAACCCATC ATGCTTTACC AGGATATGAC TGCGCGGGAC 
CTGCTACAGC AGCGGTACAC GCTCCCCAAC GGCGACACCG CCTGGCGCTC CTCACCCCTT 
GTGAGTGCTG CCCGGGAAGG CAAACTGGTC CTGTTGGATG GTATCCACCG CGTCAACGCT 
GGCACACTGG CTGTATTGCA AAGACTGATC CATGACCGGG AGCTCAGCCT GTATGATGGA 
TCTCGGCTGC TGAGGGAGGA CAGGTACCTG AGCTTGAAGG AGAAGCTACA ACTGACCGAT 
GAGCAGCTAC AGAACAGGTC CATTTTTCCT ATCCACCCTT CCTTCAGAAT CATTGCGTTG 
GCAGAGCCCC CAATTGTTGG AAGCACAACA CAGCAGTGGC TGGGACCAGA ATTTTTAACT 
ATGTTCTTTT TCCATCACAT GAAGCCTCTT GTGAAAAGTG AAGAAATCCA AGTGATAAAG 
GAGACGGTTC CAAATGTACC TCAGGAAGCA CTGGAGAAAT TGCTGTCAGT CACTCACAAA 
CTCAGGGAGA CCCAGGATCC CACAGCACAG TCCTTGGCAG CCTCGCTTTC CACCCGTCAG 
CTCTTACGGA TCTCTCGCCG GCTCTCGAAG TATCCCAGCG AAAATCTTCA TGATGCCATT 
ACCAAAGCCT GTCTTTCCAG GTTCTTACCA AGCCTTGCTC AGTCTGCACT GGAGAAGAAT 
CTGGCAGATG CGGCAATAGA AACAAACACT GAGGACAGCC TGGAGCCAGA GCTGGAGAAT 
TACAAGTGTG AAGTAGTCGC CGGGTCTCTG AAGATTGGCG CCGTCAGTGT GCCAGTGCAC 
AATGCCCATG AGAAGATGAA AGTGCCTGAC GTTCTTTTCT ACGACAATGT TCAGCACATG 
GTAGTGATGG AAGACATGCT TAAAGACTTT GTCCTTGGCG AACACTTACT ATTGGTTGGT 
AACCAGGGGG TAGGAAAGAA CAAGATTGTG GACCGATTCC TTCACCTGCT CAACAGACCC 
CGAGAATATA TACAGCTGCA CAGGGACACC ACAGTGCAGT CTCTGACTCT CCAGCCCACA 
GTGAAAGGTG GACTGATTGT ATATGAAGAC TCACCTCTGG TTAAAGCAGT AAAGTTGGGT 
CATATTCTAG TAGTAGATGA GGCAGACAAA GCCCCAACAA ATGTCACCTG TATTTTGAAG 
ACTCTGGTAG AAAACGGAGA AATGATTCTT GCGGACGGAA GACGTATTGT TGCAGATGCA 
GCAAATGTGG ATGGAAGAGA GAACCTTGTC GCGATCCATC CTGACTTCAG GATGCTGGCT 
CTGGCCAACA GGCCTGGCTT CCCCTTCTTG GGCAATGATT TCTTTGGAAC TTTAGGTGAC 
ATTTTTAGCT GTCATGCCAT TGACAACCCC AAGCCCCACT CAGAGCTTTC GATGCTCAAA 
CAGTACGGCC CAGACGTGCC TGAGCCCGTC CTGCAGAAGC TCGTGGCAGC CTTTGGAGAG 
CTGAGGAACT TGGCTGACCA AGGGATTATC AACTACCCTT ATTCCACCAG AGAAGTGGTC 
AACATCGTCA AGCACTTACA GAAATTTCCC ACTGAAGGCC TCTCCAGTGT GGTTCGGAAT 
GTATTTGACT TCGATTCTTA CAACAACGAC ATGAGGGAGA TTTTGATGAA CACTTTGCAC 
AAATATGGGA TCCCCATTGG AGCAAAGCCT ACCAACGTGC AGCTGGCAAA GGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776119.2
CDS	107..3223
Translation	MQSRLLLLGAPGGLGDVASRRVRLLLRQVLRGRPGGDQQRLEVRLLHSGATDSGETVSIGDVSYKLKTPKNPELVPQNYISDSPAQSIVQHLRWLMQKDLLGQDVFLIGPPGPLRRSVAMQYLELTKREVEYIALSRDTTETDLKQRREIRAGTAFYIDQCAVRAATEGRTLVLEGLEKAERNVLPVLNNLLENREMQLEDGRFLMSAERYDKLLQDHTKEELDAWKIVRVSENFRVIALGLPVPRYSGNPLDPPLRSRFQARDIYFLPFQDQLKLLYSVGANVSAEKISQLLSFATTLCSQESSTLGLPDFPLDSLPEAVQILDSFPMMSIEHALQWVYPYTLLLGHEGKMAVEGVLKRFELQGSGHSLLPKEIVRVERMTDSHGSYAHVTIRVAGKEVTIKVPAGTRAVNQPCAPDHFIQTVSHKQLLAEMVQSHMVKDICLIGGKGCGKTVIAKNFAALLGYSIEPIMLYQDMTARDLLQQRYTLPNGDTAWRSSPLVSAAREGKLVLLDGIHRVNAGTLAVLQRLIHDRELSLYDGSRLLREDRYLSLKEKLQLTDEQLQNRSIFPIHPSFRIIALAEPPIVGSTTQQWLGPEFLTMFFFHHMKPLVKSEEIQVIKETVPNVPQEALEKLLSVTHKLRETQDPTAQSLAASLSTRQLLRISRRLSKYPSENLHDAITKACLSRFLPSLAQSALEKNLADAAIETNTEDSLEPELENYKCEVVAGSLKIGAVSVPVHNAHEKMKVPDVLFYDNVQHMVVMEDMLKDFVLGEHLLLVGNQGVGKNKIVDRFLHLLNRPREYIQLHRDTTVQSLTLQPTVKGGLIVYEDSPLVKAVKLGHILVVDEADKAPTNVTCILKTLVENGEMILADGRRIVADAANVDGRENLVAIHPDFRMLALANRPGFPFLGNDFFGTLGDIFSCHAIDNPKPHSELSMLKQYGPDVPEPVLQKLVAAFGELRNLADQGIINYPYSTREVVNIVKHLQKFPTEGLSSVVRNVFDFDSYNNDMREILMNTLHKYGIPIGAKPTNVQLAKE

Target ORF information:

RefSeq Version	NM_173758.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus von Willebrand factor A domain containing 8 (Vwa8), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173758.3

ORF Insert Sequence:

ATGCAGTCAC GCCTCCTCCT CCTAGGAGCC CCGGGCGGCC TTGGGGACGT GGCCTCCCGG 
CGAGTGCGGC TGCTCTTGCG GCAGGTGTTG CGGGGCAGGC CGGGCGGGGA CCAGCAGCGG 
CTGGAGGTCA GGCTGCTGCA CTCTGGGGCG ACCGACTCAG GTGAAACGGT TAGTATTGGA 
GATGTGTCCT ACAAGCTGAA AACTCCCAAG AATCCAGAAC TTGTCCCACA GAACTACATT 
TCAGACTCTC CAGCTCAGTC CATAGTCCAG CATCTGAGAT GGCTGATGCA GAAGGATCTG 
CTGGGGCAGG ATGTCTTTCT CATCGGACCT CCTGGGCCTC TCCGGCGCTC GGTGGCTATG 
CAGTACTTGG AGCTGACCAA ACGAGAGGTG GAATACATCG CCCTGTCAAG GGACACCACT 
GAAACTGACC TCAAACAGCG CCGGGAGATC CGAGCTGGCA CAGCCTTTTA CATTGACCAG 
TGTGCGGTTC GGGCTGCCAC AGAAGGCAGG ACGCTGGTTT TAGAAGGCTT GGAAAAGGCG 
GAGAGAAACG TGCTTCCAGT ACTGAACAAC TTGCTGGAGA ACCGGGAGAT GCAGCTTGAA 
GATGGGCGCT TCCTCATGTC TGCGGAGCGC TATGACAAGC TTCTCCAAGA TCACACTAAA 
GAGGAGCTGG ACGCGTGGAA GATTGTCCGA GTCAGTGAGA ATTTCCGAGT GATTGCCTTG 
GGCTTGCCAG TGCCCAGGTA CTCTGGGAAT CCATTAGACC CCCCTCTCCG GTCTCGATTT 
CAAGCCAGAG ATATCTATTT TCTACCCTTT CAGGACCAAC TAAAACTATT GTATTCAGTT 
GGAGCCAATG TTTCTGCTGA GAAGATTTCT CAGCTCTTGT CCTTTGCGAC AACCCTCTGC 
TCCCAAGAAT CCTCTACGCT TGGGCTTCCA GACTTTCCCT TAGATAGTTT GCCAGAGGCA 
GTTCAAATCC TGGACTCCTT TCCCATGATG TCGATTGAAC ATGCACTCCA GTGGGTGTAT 
CCTTACACTC TTTTACTCGG ACACGAGGGG AAGATGGCCG TGGAGGGTGT TTTAAAACGC 
TTTGAGCTCC AAGGTTCTGG ACATTCCCTG CTTCCTAAGG AGATTGTAAG AGTGGAGAGG 
ATGACTGACA GCCACGGCTC CTATGCCCAC GTGACAATCC GGGTCGCAGG AAAAGAGGTG 
ACCATTAAGG TACCAGCGGG GACCAGAGCA GTAAACCAGC CTTGTGCTCC TGACCATTTC 
ATTCAGACTG TCAGTCACAA GCAATTACTG GCTGAGATGG TGCAGTCTCA TATGGTGAAG 
GACATATGCT TAATTGGAGG GAAGGGCTGT GGGAAAACGG TCATCGCTAA GAACTTTGCT 
GCTCTCCTAG GATACAGCAT AGAACCCATC ATGCTTTACC AGGATATGAC TGCGCGGGAC 
CTGCTACAGC AGCGGTACAC GCTCCCCAAC GGCGACACCG CCTGGCGCTC CTCACCCCTT 
GTGAGTGCTG CCCGGGAAGG CAAACTGGTC CTGTTGGATG GTATCCACCG CGTCAACGCT 
GGCACACTGG CTGTATTGCA AAGACTGATC CATGACCGGG AGCTCAGCCT GTATGATGGA 
TCTCGGCTGC TGAGGGAGGA CAGGTACCTG AGCTTGAAGG AGAAGCTACA ACTGACCGAT 
GAGCAGCTAC AGAACAGGTC CATTTTTCCT ATCCACCCTT CCTTCAGAAT CATTGCGTTG 
GCAGAGCCCC CAATTGTTGG AAGCACAACA CAGCAGTGGC TGGGACCAGA ATTTTTAACT 
ATGTTCTTTT TCCATCACAT GAAGCCTCTT GTGAAAAGTG AAGAAATCCA AGTGATAAAG 
GAGACGGTTC CAAATGTACC TCAGGAAGCA CTGGAGAAAT TGCTGTCAGT CACTCACAAA 
CTCAGGGAGA CCCAGGATCC CACAGCACAG TCCTTGGCAG CCTCGCTTTC CACCCGTCAG 
CTCTTACGGA TCTCTCGCCG GCTCTCGAAG TATCCCAGCG AAAATCTTCA TGATGCCATT 
ACCAAAGCCT GTCTTTCCAG GTTCTTACCA AGCCTTGCTC AGTCTGCACT GGAGAAGAAT 
CTGGCAGATG CGGCAATAGA AACAAACACT GAGGACAGCC TGGAGCCAGA GCTGGAGAAT 
TACAAGTGTG AAGTAGTCGC CGGGTCTCTG AAGATTGGCG CCGTCAGTGT GCCAGTGCAC 
AATGCCCATG AGAAGATGAA AGTGCCTGAC GTTCTTTTCT ACGACAATGT TCAGCACATG 
GTAGTGATGG AAGACATGCT TAAAGACTTT GTCCTTGGCG AACACTTACT ATTGGTTGGT 
AACCAGGGGG TAGGAAAGAA CAAGATTGTG GACCGATTCC TTCACCTGCT CAACAGACCC 
CGAGAATATA TACAGCTGCA CAGGGACACC ACAGTGCAGT CTCTGACTCT CCAGCCCACA 
GTGAAAGGTG GACTGATTGT ATATGAAGAC TCACCTCTGG TTAAAGCAGT AAAGTTGGGT 
CATATTCTAG TAGTAGATGA GGCAGACAAA GCCCCAACAA ATGTCACCTG TATTTTGAAG 
ACTCTGGTAG AAAACGGAGA AATGATTCTT GCGGACGGAA GACGTATTGT TGCAGATGCA 
GCAAATGTGG ATGGAAGAGA GAACCTTGTC GCGATCCATC CTGACTTCAG GATGCTGGCT 
CTGGCCAACA GGCCTGGCTT CCCCTTCTTG GGCAATGATT TCTTTGGAAC TTTAGGTGAC 
ATTTTTAGCT GTCATGCCAT TGACAACCCC AAGCCCCACT CAGAGCTTTC GATGCTCAAA 
CAGTACGGCC CAGACGTGCC TGAGCCCGTC CTGCAGAAGC TCGTGGCAGC CTTTGGAGAG 
CTGAGGAACT TGGCTGACCA AGGGATTATC AACTACCCTT ATTCCACCAG AGAAGTGGTC 
AACATCGTCA AGCACTTACA GAAATTTCCC ACTGAAGGCC TCTCCAGTGT GGTTCGGAAT 
GTATTTGACT TCGATTCTTA CAACAACGAC ATGAGGGAGA TTTTGATGAA CACTTTGCAC 
AAATATGGGA TCCCCATTGG AGCAAAGCCT ACCAACGTGC AGCTGGCAAA GGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vwa8 ( XM_011245038.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011243340.1 Mus musculus von Willebrand factor A domain containing 8 (Vwa8), transcript variant X3, mRNA.

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CloneID	OMu73110
Clone ID Related Accession (Same CDS sequence)	XM_011245038.2 , XM_011245038.3
Accession Version	XM_011245038.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	von Willebrand factor A domain-containing protein 8 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011245038.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCAC GCCTCCTCCT CCTAGGAGCC CCGGGCGGCC TTGGGGACGT GGCCTCCCGG 
CGAGTGCGGC TGCTCTTGCG GCAGGTGTTG CGGGGCAGGC CGGGCGGGGA CCAGCAGCGG 
CTGGAGGTCA GGCTGCTGCA CTCTGGGGCG ACCGACTCAG GTGAAACGGT TAGTATTGGA 
GATGTGTCCT ACAAGCTGAA AACTCCCAAG AATCCAGAAC TTGTCCCACA GAACTACATT 
TCAGACTCTC CAGCTCAGTC CATAGTCCAG CATCTGAGAT GGCTGATGCA GAAGGATCTG 
CTGGGGCAGG ATGTCTTTCT CATCGGACCT CCTGGGCCTC TCCGGCGCTC GGTGGCTATG 
CAGTACTTGG AGCTGACCAA ACGAGAGGTG GAATACATCG CCCTGTCAAG GGACACCACT 
GAAACTGACC TCAAACAGCG CCGGGAGATC CGAGCTGGCA CAGCCTTTTA CATTGACCAG 
TGTGCGGTTC GGGCTGCCAC AGAAGGCAGG ACGCTGGTTT TAGAAGGCTT GGAAAAGGCG 
GAGAGAAACG TGCTTCCAGT ACTGAACAAC TTGCTGGAGA ACCGGGAGAT GCAGCTTGAA 
GATGGGCGCT TCCTCATGTC TGCGGAGCGC TATGACAAGC TTCTCCAAGA TCACACTAAA 
GAGGAGCTGG ACGCGTGGAA GATTGTCCGA GTCAGTGAGA ATTTCCGAGT GATTGCCTTG 
GGCTTGCCAG TGCCCAGGTA CTCTGGGAAT CCATTAGACC CCCCTCTCCG GTCTCGATTT 
CAAGCCAGAG ATATCTATTT TCTACCCTTT CAGGACCAAC TAAAACTATT GTATTCAGTT 
GGAGCCAATG TTTCTGCTGA GAAGATTTCT CAGCTCTTGT CCTTTGCGAC AACCCTCTGC 
TCCCAAGAAT CCTCTACGCT TGGGCTTCCA GACTTTCCCT TAGATAGTTT GCCAGAGGCA 
GTTCAAATCC TGGACTCCTT TCCCATGATG TCGATTGAAC ATGCACTCCA GTGGGTGTAT 
CCTTACACTC TTTTACTCGG ACACGAGGGG AAGATGGCCG TGGAGGGTGT TTTAAAACGC 
TTTGAGCTCC AAGGTTCTGG ACATTCCCTG CTTCCTAAGG AGATTGTAAG AGTGGAGAGG 
ATGACTGACA GCCACGGCTC CTATGCCCAC GTGACAATCC GGGTCGCAGG AAAAGAGGTG 
ACCATTAAGG TACCAGCGGG GACCAGAGCA GTAAACCAGC CTTGTGCTCC TGACCATTTC 
ATTCAGACTG TCAGTCACAA GCAATTACTG GCTGAGATGG TGCAGTCTCA TATGGTGAAG 
GACATATGCT TAATTGGAGG GAAGGGCTGT GGGAAAACGG TCATCGCTAA GAACTTTGCT 
GCTCTCCTAG GATACAGCAT AGAACCCATC ATGCTTTACC AGGATATGAC TGCGCGGGAC 
CTGCTACAGC AGCGGTACAC GCTCCCCAAC GGCGACACCG CCTGGCGCTC CTCACCCCTT 
GTGAGTGCTG CCCGGGAAGG CAAACTGGTC CTGTTGGATG GTATCCACCG CGTCAACGCT 
GGCACACTGG CTGTATTGCA AAGACTGATC CATGACCGGG AGCTCAGCCT GTATGATGGA 
TCTCGGCTGC TGAGGGAGGA CAGGTACCTG AGCTTGAAGG AGAAGCTACA ACTGACCGAT 
GAGCAGCTAC AGAACAGGTC CATTTTTCCT ATCCACCCTT CCTTCAGAAT CATTGCGTTG 
GCAGAGCCCC CAATTGTTGG AAGCACAACA CAGCAGTGGC TGGGACCAGA ATTTTTAACT 
ATGTTCTTTT TCCATCACAT GAAGCCTCTT GTGAAAAGTG AAGAAATCCA AGTGATAAAG 
GAGACGGTTC CAAATGTACC TCAGGAAGCA CTGGAGAAAT TGCTGTCAGT CACTCACAAA 
CTCAGGGAGA CCCAGGATCC CACAGCACAG TCCTTGGCAG CCTCGCTTTC CACCCGTCAG 
CTCTTACGGA TCTCTCGCCG GCTCTCGAAG TATCCCAGCG AAAATCTTCA TGATGCCATT 
ACCAAAGCCT GTCTTTCCAG GTTCTTACCA AGCCTTGCTC AGTCTGCACT GGAGAAGAAT 
CTGGCAGATG CGGCAATAGA AACAAACACT GAGGACAGCC TGGAGCCAGA GCTGGAGAAT 
TACAAGTGTG AAGTAGTCGC CGGGTCTCTG AAGATTGGCG CCGTCAGTGT GCCAGTGCAC 
AATGCCCATG AGAAGATGAA AGTGCCTGAC GTTCTTTTCT ACGACAATGT TCAGCACATG 
GTAGTGATGG AAGACATGCT TAAAGACTTT GTCCTTGGCG AACACTTACT ATTGGTTGGT 
AACCAGGGGG TAGGAAAGAA CAAGATTGTG GACCGATTCC TTCACCTGCT CAACAGACCC 
CGAGAATATA TACAGCTGCA CAGGGACACC ACAGTGCAGT CTCTGACTCT CCAGCCCACA 
GTGAAAGGTG GACTGATTGT ATATGAAGAC TCACCTCTGG TTAAAGCAGT AAAGTTGGGT 
CATATTCTAG TAGTAGATGA GGCAGACAAA GCCCCAACAA ATGTCACCTG TATTTTGAAG 
ACTCTGGTAG AAAACGGAGA AATGATTCTT GCGGACGGAA GACGTATTGT TGCAGATGCA 
GCAAATGTGG ATGGAAGAGA GAACCTTGTC GCGATCCATC CTGACTTCAG GATGCTGGCT 
CTGGCCAACA GGCCTGGCTT CCCCTTCTTG GGCAATGATT TCTTTGGAAC TTTAGGTGAC 
ATTTTTAGCT GTCATGCCAT TGACAACCCC AAGCCCCACT CAGAGCTTTC GATGCTCAAA 
CAGTACGGCC CAGACGTGCC TGAGCCCGTC CTGCAGAAGC TCGTGGCAGC CTTTGGAGAG 
CTGAGGAACT TGGCTGACCA AGGGATTATC AACTACCCTT ATTCCACCAG AGAAGTGGTC 
AACATCGTCA AGCACTTACA GAAATTTCCC ACTGAAGGCC TCTCCAGTGT GGTTCGGAAT 
GTATTTGACT TCGATTCTTA CAACAACGAC ATGAGGGAGA TTTTGATGAA CACTTTGCAC 
AAATATGGGA TCCCCATTGG AGCAAAGCCT ACCAACGTGC AGCTGGCAAA GGAGTTTCCT 
CTGCCAGAGA AGACATTCAT GGGCTACTGG ATAGTTGGCC AGACGGGAAA TGGAATGCAG 
AAGGTCTTGT GTCCAGCTGA AACAAATCAC GTTGACATAA AGGGCCCAGT ACTTGTGAAC 
ATGGAAAAGT ACCCAATAGA AAAACATGAA GCGAGATTCC TAAGCTTTAC TGAAGAGTGT 
ACGTCCTGGA AGTTTCCATT GGATGAAGTC AATTTAATCT GTGACATCGC TGTATCACAT 
GTCATAGCAC AAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011243340.1
CDS	226..3603
Translation	MQSRLLLLGAPGGLGDVASRRVRLLLRQVLRGRPGGDQQRLEVRLLHSGATDSGETVSIGDVSYKLKTPKNPELVPQNYISDSPAQSIVQHLRWLMQKDLLGQDVFLIGPPGPLRRSVAMQYLELTKREVEYIALSRDTTETDLKQRREIRAGTAFYIDQCAVRAATEGRTLVLEGLEKAERNVLPVLNNLLENREMQLEDGRFLMSAERYDKLLQDHTKEELDAWKIVRVSENFRVIALGLPVPRYSGNPLDPPLRSRFQARDIYFLPFQDQLKLLYSVGANVSAEKISQLLSFATTLCSQESSTLGLPDFPLDSLPEAVQILDSFPMMSIEHALQWVYPYTLLLGHEGKMAVEGVLKRFELQGSGHSLLPKEIVRVERMTDSHGSYAHVTIRVAGKEVTIKVPAGTRAVNQPCAPDHFIQTVSHKQLLAEMVQSHMVKDICLIGGKGCGKTVIAKNFAALLGYSIEPIMLYQDMTARDLLQQRYTLPNGDTAWRSSPLVSAAREGKLVLLDGIHRVNAGTLAVLQRLIHDRELSLYDGSRLLREDRYLSLKEKLQLTDEQLQNRSIFPIHPSFRIIALAEPPIVGSTTQQWLGPEFLTMFFFHHMKPLVKSEEIQVIKETVPNVPQEALEKLLSVTHKLRETQDPTAQSLAASLSTRQLLRISRRLSKYPSENLHDAITKACLSRFLPSLAQSALEKNLADAAIETNTEDSLEPELENYKCEVVAGSLKIGAVSVPVHNAHEKMKVPDVLFYDNVQHMVVMEDMLKDFVLGEHLLLVGNQGVGKNKIVDRFLHLLNRPREYIQLHRDTTVQSLTLQPTVKGGLIVYEDSPLVKAVKLGHILVVDEADKAPTNVTCILKTLVENGEMILADGRRIVADAANVDGRENLVAIHPDFRMLALANRPGFPFLGNDFFGTLGDIFSCHAIDNPKPHSELSMLKQYGPDVPEPVLQKLVAAFGELRNLADQGIINYPYSTREVVNIVKHLQKFPTEGLSSVVRNVFDFDSYNNDMREILMNTLHKYGIPIGAKPTNVQLAKEFPLPEKTFMGYWIVGQTGNGMQKVLCPAETNHVDIKGPVLVNMEKYPIEKHEARFLSFTEECTSWKFPLDEVNLICDIAVSHVIAQS

Target ORF information:

RefSeq Version	XM_011245038.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus von Willebrand factor A domain containing 8 (Vwa8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011245038.2

ORF Insert Sequence:

ATGCAGTCAC GCCTCCTCCT CCTAGGAGCC CCGGGCGGCC TTGGGGACGT GGCCTCCCGG 
CGAGTGCGGC TGCTCTTGCG GCAGGTGTTG CGGGGCAGGC CGGGCGGGGA CCAGCAGCGG 
CTGGAGGTCA GGCTGCTGCA CTCTGGGGCG ACCGACTCAG GTGAAACGGT TAGTATTGGA 
GATGTGTCCT ACAAGCTGAA AACTCCCAAG AATCCAGAAC TTGTCCCACA GAACTACATT 
TCAGACTCTC CAGCTCAGTC CATAGTCCAG CATCTGAGAT GGCTGATGCA GAAGGATCTG 
CTGGGGCAGG ATGTCTTTCT CATCGGACCT CCTGGGCCTC TCCGGCGCTC GGTGGCTATG 
CAGTACTTGG AGCTGACCAA ACGAGAGGTG GAATACATCG CCCTGTCAAG GGACACCACT 
GAAACTGACC TCAAACAGCG CCGGGAGATC CGAGCTGGCA CAGCCTTTTA CATTGACCAG 
TGTGCGGTTC GGGCTGCCAC AGAAGGCAGG ACGCTGGTTT TAGAAGGCTT GGAAAAGGCG 
GAGAGAAACG TGCTTCCAGT ACTGAACAAC TTGCTGGAGA ACCGGGAGAT GCAGCTTGAA 
GATGGGCGCT TCCTCATGTC TGCGGAGCGC TATGACAAGC TTCTCCAAGA TCACACTAAA 
GAGGAGCTGG ACGCGTGGAA GATTGTCCGA GTCAGTGAGA ATTTCCGAGT GATTGCCTTG 
GGCTTGCCAG TGCCCAGGTA CTCTGGGAAT CCATTAGACC CCCCTCTCCG GTCTCGATTT 
CAAGCCAGAG ATATCTATTT TCTACCCTTT CAGGACCAAC TAAAACTATT GTATTCAGTT 
GGAGCCAATG TTTCTGCTGA GAAGATTTCT CAGCTCTTGT CCTTTGCGAC AACCCTCTGC 
TCCCAAGAAT CCTCTACGCT TGGGCTTCCA GACTTTCCCT TAGATAGTTT GCCAGAGGCA 
GTTCAAATCC TGGACTCCTT TCCCATGATG TCGATTGAAC ATGCACTCCA GTGGGTGTAT 
CCTTACACTC TTTTACTCGG ACACGAGGGG AAGATGGCCG TGGAGGGTGT TTTAAAACGC 
TTTGAGCTCC AAGGTTCTGG ACATTCCCTG CTTCCTAAGG AGATTGTAAG AGTGGAGAGG 
ATGACTGACA GCCACGGCTC CTATGCCCAC GTGACAATCC GGGTCGCAGG AAAAGAGGTG 
ACCATTAAGG TACCAGCGGG GACCAGAGCA GTAAACCAGC CTTGTGCTCC TGACCATTTC 
ATTCAGACTG TCAGTCACAA GCAATTACTG GCTGAGATGG TGCAGTCTCA TATGGTGAAG 
GACATATGCT TAATTGGAGG GAAGGGCTGT GGGAAAACGG TCATCGCTAA GAACTTTGCT 
GCTCTCCTAG GATACAGCAT AGAACCCATC ATGCTTTACC AGGATATGAC TGCGCGGGAC 
CTGCTACAGC AGCGGTACAC GCTCCCCAAC GGCGACACCG CCTGGCGCTC CTCACCCCTT 
GTGAGTGCTG CCCGGGAAGG CAAACTGGTC CTGTTGGATG GTATCCACCG CGTCAACGCT 
GGCACACTGG CTGTATTGCA AAGACTGATC CATGACCGGG AGCTCAGCCT GTATGATGGA 
TCTCGGCTGC TGAGGGAGGA CAGGTACCTG AGCTTGAAGG AGAAGCTACA ACTGACCGAT 
GAGCAGCTAC AGAACAGGTC CATTTTTCCT ATCCACCCTT CCTTCAGAAT CATTGCGTTG 
GCAGAGCCCC CAATTGTTGG AAGCACAACA CAGCAGTGGC TGGGACCAGA ATTTTTAACT 
ATGTTCTTTT TCCATCACAT GAAGCCTCTT GTGAAAAGTG AAGAAATCCA AGTGATAAAG 
GAGACGGTTC CAAATGTACC TCAGGAAGCA CTGGAGAAAT TGCTGTCAGT CACTCACAAA 
CTCAGGGAGA CCCAGGATCC CACAGCACAG TCCTTGGCAG CCTCGCTTTC CACCCGTCAG 
CTCTTACGGA TCTCTCGCCG GCTCTCGAAG TATCCCAGCG AAAATCTTCA TGATGCCATT 
ACCAAAGCCT GTCTTTCCAG GTTCTTACCA AGCCTTGCTC AGTCTGCACT GGAGAAGAAT 
CTGGCAGATG CGGCAATAGA AACAAACACT GAGGACAGCC TGGAGCCAGA GCTGGAGAAT 
TACAAGTGTG AAGTAGTCGC CGGGTCTCTG AAGATTGGCG CCGTCAGTGT GCCAGTGCAC 
AATGCCCATG AGAAGATGAA AGTGCCTGAC GTTCTTTTCT ACGACAATGT TCAGCACATG 
GTAGTGATGG AAGACATGCT TAAAGACTTT GTCCTTGGCG AACACTTACT ATTGGTTGGT 
AACCAGGGGG TAGGAAAGAA CAAGATTGTG GACCGATTCC TTCACCTGCT CAACAGACCC 
CGAGAATATA TACAGCTGCA CAGGGACACC ACAGTGCAGT CTCTGACTCT CCAGCCCACA 
GTGAAAGGTG GACTGATTGT ATATGAAGAC TCACCTCTGG TTAAAGCAGT AAAGTTGGGT 
CATATTCTAG TAGTAGATGA GGCAGACAAA GCCCCAACAA ATGTCACCTG TATTTTGAAG 
ACTCTGGTAG AAAACGGAGA AATGATTCTT GCGGACGGAA GACGTATTGT TGCAGATGCA 
GCAAATGTGG ATGGAAGAGA GAACCTTGTC GCGATCCATC CTGACTTCAG GATGCTGGCT 
CTGGCCAACA GGCCTGGCTT CCCCTTCTTG GGCAATGATT TCTTTGGAAC TTTAGGTGAC 
ATTTTTAGCT GTCATGCCAT TGACAACCCC AAGCCCCACT CAGAGCTTTC GATGCTCAAA 
CAGTACGGCC CAGACGTGCC TGAGCCCGTC CTGCAGAAGC TCGTGGCAGC CTTTGGAGAG 
CTGAGGAACT TGGCTGACCA AGGGATTATC AACTACCCTT ATTCCACCAG AGAAGTGGTC 
AACATCGTCA AGCACTTACA GAAATTTCCC ACTGAAGGCC TCTCCAGTGT GGTTCGGAAT 
GTATTTGACT TCGATTCTTA CAACAACGAC ATGAGGGAGA TTTTGATGAA CACTTTGCAC 
AAATATGGGA TCCCCATTGG AGCAAAGCCT ACCAACGTGC AGCTGGCAAA GGAGTTTCCT 
CTGCCAGAGA AGACATTCAT GGGCTACTGG ATAGTTGGCC AGACGGGAAA TGGAATGCAG 
AAGGTCTTGT GTCCAGCTGA AACAAATCAC GTTGACATAA AGGGCCCAGT ACTTGTGAAC 
ATGGAAAAGT ACCCAATAGA AAAACATGAA GCGAGATTCC TAAGCTTTAC TGAAGAGTGT 
ACGTCCTGGA AGTTTCCATT GGATGAAGTC AATTTAATCT GTGACATCGC TGTATCACAT 
GTCATAGCAC AAAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vwa8 ( NM_027906.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_082182.1 Mus musculus von Willebrand factor A domain containing 8 (Vwa8), transcript variant 1, mRNA.

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CloneID	OMu04758
Clone ID Related Accession (Same CDS sequence)	NM_027906.1
Accession Version	NM_027906.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5718bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Mus musculus(house mouse)
Product	von Willebrand factor A domain-containing protein 8 isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC155167.4, AC124709.3 and AC139758.3. On or before Apr 19, 2009 this sequence version replaced XM_915363.3, XM_001002205.2. Transcript Variant: This variant (1) represents the longer transcript and encodes a longer protein isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGTCAC GCCTCCTCCT CCTAGGAGCC CCGGGCGGCC TTGGGGACGT GGCCTCCCGG 
CGAGTGCGGC TGCTCTTGCG GCAGGTGTTG CGGGGCAGGC CGGGCGGGGA CCAGCAGCGG 
CTGGAGGTCA GGCTGCTGCA CTCTGGGGCG ACCGACTCAG GTGAAACGGT TAGTATTGGA 
GATGTGTCCT ACAAGCTGAA AACTCCCAAG AATCCAGAAC TTGTCCCACA GAACTACATT 
TCAGACTCTC CAGCTCAGTC CATAGTCCAG CATCTGAGAT GGCTGATGCA GAAGGATCTG 
CTGGGGCAGG ATGTCTTTCT CATCGGACCT CCTGGGCCTC TCCGGCGCTC GGTGGCTATG 
CAGTACTTGG AGCTGACCAA ACGAGAGGTG GAATACATCG CCCTGTCAAG GGACACCACT 
GAAACTGACC TCAAACAGCG CCGGGAGATC CGAGCTGGCA CAGCCTTTTA CATTGACCAG 
TGTGCGGTTC GGGCTGCCAC AGAAGGCAGG ACGCTGGTTT TAGAAGGCTT GGAAAAGGCG 
GAGAGAAACG TGCTTCCAGT ACTGAACAAC TTGCTGGAGA ACCGGGAGAT GCAGCTTGAA 
GATGGGCGCT TCCTCATGTC TGCGGAGCGC TATGACAAGC TTCTCCAAGA TCACACTAAA 
GAGGAGCTGG ACGCGTGGAA GATTGTCCGA GTCAGTGAGA ATTTCCGAGT GATTGCCTTG 
GGCTTGCCAG TGCCCAGGTA CTCTGGGAAT CCATTAGACC CCCCTCTCCG GTCTCGATTT 
CAAGCCAGAG ATATCTATTT TCTACCCTTT CAGGACCAAC TAAAACTATT GTATTCAGTT 
GGAGCCAATG TTTCTGCTGA GAAGATTTCT CAGCTCTTGT CCTTTGCGAC AACCCTCTGC 
TCCCAAGAAT CCTCTACGCT TGGGCTTCCA GACTTTCCCT TAGATAGTTT GCCAGAGGCA 
GTTCAAATCC TGGACTCCTT TCCCATGATG TCGATTGAAC ATGCACTCCA GTGGGTGTAT 
CCTTACACTC TTTTACTCGG ACACGAGGGG AAGATGGCCG TGGAGGGTGT TTTAAAACGC 
TTTGAGCTCC AAGGTTCTGG ACATTCCCTG CTTCCTAAGG AGATTGTAAG AGTGGAGAGG 
ATGACTGACA GCCACGGCTC CTATGCCCAC GTGACAATCC GGGTCGCAGG AAAAGAGGTG 
ACCATTAAGG TACCAGCGGG GACCAGAGCA GTAAACCAGC CTTGTGCTCC TGACCATTTC 
ATTCAGACTG TCAGTCACAA GCAATTACTG GCTGAGATGG TGCAGTCTCA TATGGTGAAG 
GACATATGCT TAATTGGAGG GAAGGGCTGT GGGAAAACGG TCATCGCTAA GAACTTTGCT 
GCTCTCCTAG GATACAGCAT AGAACCCATC ATGCTTTACC AGGATATGAC TGCGCGGGAC 
CTGCTACAGC AGCGGTACAC GCTCCCCAAC GGCGACACCG CCTGGCGCTC CTCACCCCTT 
GTGAGTGCTG CCCGGGAAGG CAAACTGGTC CTGTTGGATG GTATCCACCG CGTCAACGCT 
GGCACACTGG CTGTATTGCA AAGACTGATC CATGACCGGG AGCTCAGCCT GTATGATGGA 
TCTCGGCTGC TGAGGGAGGA CAGGTACCTG AGCTTGAAGG AGAAGCTACA ACTGACCGAT 
GAGCAGCTAC AGAACAGGTC CATTTTTCCT ATCCACCCTT CCTTCAGAAT CATTGCGTTG 
GCAGAGCCCC CAATTGTTGG AAGCACAACA CAGCAGTGGC TGGGACCAGA ATTTTTAACT 
ATGTTCTTTT TCCATCACAT GAAGCCTCTT GTGAAAAGTG AAGAAATCCA AGTGATAAAG 
GAGACGGTTC CAAATGTACC TCAGGAAGCA CTGGAGAAAT TGCTGTCAGT CACTCACAAA 
CTCAGGGAGA CCCAGGATCC CACAGCACAG TCCTTGGCAG CCTCGCTTTC CACCCGTCAG 
CTCTTACGGA TCTCTCGCCG GCTCTCGAAG TATCCCAGCG AAAATCTTCA TGATGCCATT 
ACCAAAGCCT GTCTTTCCAG GTTCTTACCA AGCCTTGCTC AGTCTGCACT GGAGAAGAAT 
CTGGCAGATG CGGCAATAGA AACAAACACT GAGGACAGCC TGGAGCCAGA GCTGGAGAAT 
TACAAGTGTG AAGTAGTCGC CGGGTCTCTG AAGATTGGCG CCGTCAGTGT GCCAGTGCAC 
AATGCCCATG AGAAGATGAA AGTGCCTGAC GTTCTTTTCT ACGACAATGT TCAGCACATG 
GTAGTGATGG AAGACATGCT TAAAGACTTT GTCCTTGGCG AACACTTACT ATTGGTTGGT 
AACCAGGGGG TAGGAAAGAA CAAGATTGTG GACCGATTCC TTCACCTGCT CAACAGACCC 
CGAGAATATA TACAGCTGCA CAGGGACACC ACAGTGCAGT CTCTGACTCT CCAGCCCACA 
GTGAAAGGTG GACTGATTGT ATATGAAGAC TCACCTCTGG TTAAAGCAGT AAAGTTGGGT 
CATATTCTAG TAGTAGATGA GGCAGACAAA GCCCCAACAA ATGTCACCTG TATTTTGAAG 
ACTCTGGTAG AAAACGGAGA AATGATTCTT GCGGACGGAA GACGTATTGT TGCAGATGCA 
GCAAATGTGG ATGGAAGAGA GAACCTTGTC GCGATCCATC CTGACTTCAG GATGCTGGCT 
CTGGCCAACA GGCCTGGCTT CCCCTTCTTG GGCAATGATT TCTTTGGAAC TTTAGGTGAC 
ATTTTTAGCT GTCATGCCAT TGACAACCCC AAGCCCCACT CAGAGCTTTC GATGCTCAAA 
CAGTACGGCC CAGACGTGCC TGAGCCCGTC CTGCAGAAGC TCGTGGCAGC CTTTGGAGAG 
CTGAGGAACT TGGCTGACCA AGGGATTATC AACTACCCTT ATTCCACCAG AGAAGTGGTC 
AACATCGTCA AGCACTTACA GAAATTTCCC ACTGAAGGCC TCTCCAGTGT GGTTCGGAAT 
GTATTTGACT TCGATTCTTA CAACAACGAC ATGAGGGAGA TTTTGATGAA CACTTTGCAC 
AAATATGGGA TCCCCATTGG AGCAAAGCCT ACCAACGTGC AGCTGGCAAA GGAGTTTCCT 
CTGCCAGAGA AGACATTCAT GGGCTACTGG ATAGTTGGCC AGACGGGAAA TGGAATGCAG 
AAGGTCTTGT GTCCAGCTGA AACAAATCAC GTTGACATAA AGGGCCCAGT ACTTGTGAAC 
ATGGAAAAGT ACCCAATAGA AAAACATGAA GCGAGATTCC TAAGCTTTAC TGAAGAGTGT 
ACGTCCTGGA AGTTTCCATT GGATGAAGTC AATTTAATCT GTGACATCGC TGTATCACAT 
GAAAACGGAG AACAGACTCT GTATGTGGCT GCGTGCAACC CGGTTTCCTT GTACTTCATG 
AATATGACAG GGAAAAATGG CTTCTTTGTG GACTTCTTCG ACATCTTCCC AAGGATGGCC 
AGTGGGTCCT GGCGACCATT TGTAACAGTA GCCCCACTGG GAAGTCCTCT GAGGGGTCAA 
GTTGTTCTGC ATGAGGAGCA GAGTAATGCC GTCCTCTTAC TCGACACTAC TGGCAGTGCC 
ATTCGCCGTC TCGTCCTTCC CACAGAAGAG TTTACATCTA AGAAGTCTTC ATGGTGGAGC 
AAGGAGGAAG GTGAAACTTA TAGAATGTGT AAAGAATTCT CACACAAAAA CTGGGTAGTA 
TTCTACAAAC AAACAGGGAA TAGCTTGACT GTCCTGGATG TCTTAGAAGG ACTAGCTCAC 
ACCATCTCAC TTCCCATCAA CCTCAGGACA GTTTTCCTGG TGGCGGAGGA CAAATGGCTT 
CTTGTGGAAA ACGAAACAAA TCAGAAGTAT CTTTTGACTA AGCCTGCACA CATTGGTTCT 
GAGGACACTG GGGCCTGTCA GTTGTATATG TTGAAAGAAG AGCTGCCTAG CACAGGATTT 
GGAGTCACAC AAGAAACAGA GTTCTGCATA CCTGATAAAG TGTCAAGTGA TCAGCTGTCA 
TCTGAAAACC TAACTTCAGC TGTGGGCCAG AAGATCGCCT CTCCTAACCG TATTCTCTCG 
GATGAAAATA GCCACGCTAC CATAGTTGTT GGCTTCCCGG ACCTCATGTC ACCCAGTGAA 
GTTTATTCAT GGAAGAGGTC TTCGTCTTTG AGACAACCAA GTGTTACCAG CATGACAATG 
TACACAGGGA AGCGGACAAA TGCCACCCCA CGGCACAACA ACTGCGTGAC TCTCACACAT 
ACTAATCAGG TGGTGAGGAT TCTGCCCCCA GGAGAAGTCC CTCTGAAAGA CCTCTACCCA 
AAAGATGTTA CTCCCCCCCA GACAGCTGGC TACATAGAGG TTACTGATCT CCAAGCAAAG 
AAACTCCGTT ACATCCCTGT CCCCAGCGCA GAGTCTCTCT CGCCATACAC CGCATGGATA 
TCTGCTATTT CAGACACAGA TGCTCTGCTG GCTGAGTGGG ACAAGAGCAG CGTCGTGACT 
GTTGATATGG GAGGGCGTGT CAGGCTCTGG GAAACTGGAC TTGAACGTCT GCAGCAGTCG 
CTCATGGAAT GGAGAAACAT GATTGGACAA GACAGTGACA AACATGTGCA GATAACAATC 
GAAAGAGACA GTAATGAAGA CGTGAGTGAC CCCAAACATG GCAAGGAGGA CCCAGACAAC 
ATGCCCCATG TGGGTGGCAA TACCTGGGCT GGTGGAACAG GGGGAAGAGA CACCGCAGGT 
CTTGGTGGCA AAGGAGGTCC TTATCGCTTG GATGCAGGCC ACCCAGTGTA TCAAGTCTCT 
GAGGTGGAGA AAGATGCAGT TCCTGAGGAT GTCAAGAGAG CTGCAAGAGA GATGGCCCAG 
AAAGCATTTC AGCAGAGACT AAAGGAGATC CAGATGAGCG AGTACGACGC CGCAACCTAC 
GAGCGGTTTT CAAGTGCTGT CCAGCGGCAG GTGCATGCTC TCCGCATCAT CCTGGATAAT 
CTACAGGCTA AAGGCAAAGA AAGACAGTGG CTAAGACACC AAGCTACCGG AGAACTGGAC 
GACGCCAAGA TCATTGACGG GCTGGCGGGA GAAAAGTCCA TCTACAAACG TCGGGGTGAC 
CTGGAACCCC AGCTGGGTAG TCCACAACAG AAGCCCAAGC GCCTGCGCCT GGTGGTGGAC 
GTCTCCGGCA GCATGTACCG CTTCAACGGG GTGGATCGCC GGCTAGAGCG CTCGATGGAG 
GCAGTGTGCA TGGTCATGGA AGCCTTCGAG AACTACGAGG AGAAATTCAA GTACGACATC 
GCTGGACACT CGGGAGATGG CTACAACATT AAGCTCGTTC CAGTTAACCA AATCCCCAAG 
AACAATAAGC AGAGACTAGA AATTCTGAAG ACGATGCACG AGCACTCTCA GTTCTGCATG 
AGTGGGGACC ACACACTTGA GGGAACGGAA CACGCCATCA AGGACATTAC CACAGAAGAA 
GCTGATGAGT ACTTTGTCAT CATCTTAAGT GATGCAAATC TGTCCCGATA CGGAATAAAC 
CCGGCCCGCT TTGCCCAGAT CCTGACAAGC GATCCTCAAG TAAACGCCTT TGCCATTTTT 
ATCGGCTCTT TGGGGGATCA GGCAGCCAGG CTTCAGAGAA CTCTGCCAGC TGGCCGGTCC 
TTCATCGCTA TGGATACCAA GAAGATCCCT CAGATCTTAC AACAGATCTT CACCTCCACC 
ATGCTGTCCA GTATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_082182.1
CDS	107..5824
Translation	MQSRLLLLGAPGGLGDVASRRVRLLLRQVLRGRPGGDQQRLEVRLLHSGATDSGETVSIGDVSYKLKTPKNPELVPQNYISDSPAQSIVQHLRWLMQKDLLGQDVFLIGPPGPLRRSVAMQYLELTKREVEYIALSRDTTETDLKQRREIRAGTAFYIDQCAVRAATEGRTLVLEGLEKAERNVLPVLNNLLENREMQLEDGRFLMSAERYDKLLQDHTKEELDAWKIVRVSENFRVIALGLPVPRYSGNPLDPPLRSRFQARDIYFLPFQDQLKLLYSVGANVSAEKISQLLSFATTLCSQESSTLGLPDFPLDSLPEAVQILDSFPMMSIEHALQWVYPYTLLLGHEGKMAVEGVLKRFELQGSGHSLLPKEIVRVERMTDSHGSYAHVTIRVAGKEVTIKVPAGTRAVNQPCAPDHFIQTVSHKQLLAEMVQSHMVKDICLIGGKGCGKTVIAKNFAALLGYSIEPIMLYQDMTARDLLQQRYTLPNGDTAWRSSPLVSAAREGKLVLLDGIHRVNAGTLAVLQRLIHDRELSLYDGSRLLREDRYLSLKEKLQLTDEQLQNRSIFPIHPSFRIIALAEPPIVGSTTQQWLGPEFLTMFFFHHMKPLVKSEEIQVIKETVPNVPQEALEKLLSVTHKLRETQDPTAQSLAASLSTRQLLRISRRLSKYPSENLHDAITKACLSRFLPSLAQSALEKNLADAAIETNTEDSLEPELENYKCEVVAGSLKIGAVSVPVHNAHEKMKVPDVLFYDNVQHMVVMEDMLKDFVLGEHLLLVGNQGVGKNKIVDRFLHLLNRPREYIQLHRDTTVQSLTLQPTVKGGLIVYEDSPLVKAVKLGHILVVDEADKAPTNVTCILKTLVENGEMILADGRRIVADAANVDGRENLVAIHPDFRMLALANRPGFPFLGNDFFGTLGDIFSCHAIDNPKPHSELSMLKQYGPDVPEPVLQKLVAAFGELRNLADQGIINYPYSTREVVNIVKHLQKFPTEGLSSVVRNVFDFDSYNNDMREILMNTLHKYGIPIGAKPTNVQLAKEFPLPEKTFMGYWIVGQTGNGMQKVLCPAETNHVDIKGPVLVNMEKYPIEKHEARFLSFTEECTSWKFPLDEVNLICDIAVSHENGEQTLYVAACNPVSLYFMNMTGKNGFFVDFFDIFPRMASGSWRPFVTVAPLGSPLRGQVVLHEEQSNAVLLLDTTGSAIRRLVLPTEEFTSKKSSWWSKEEGETYRMCKEFSHKNWVVFYKQTGNSLTVLDVLEGLAHTISLPINLRTVFLVAEDKWLLVENETNQKYLLTKPAHIGSEDTGACQLYMLKEELPSTGFGVTQETEFCIPDKVSSDQLSSENLTSAVGQKIASPNRILSDENSHATIVVGFPDLMSPSEVYSWKRSSSLRQPSVTSMTMYTGKRTNATPRHNNCVTLTHTNQVVRILPPGEVPLKDLYPKDVTPPQTAGYIEVTDLQAKKLRYIPVPSAESLSPYTAWISAISDTDALLAEWDKSSVVTVDMGGRVRLWETGLERLQQSLMEWRNMIGQDSDKHVQITIERDSNEDVSDPKHGKEDPDNMPHVGGNTWAGGTGGRDTAGLGGKGGPYRLDAGHPVYQVSEVEKDAVPEDVKRAAREMAQKAFQQRLKEIQMSEYDAATYERFSSAVQRQVHALRIILDNLQAKGKERQWLRHQATGELDDAKIIDGLAGEKSIYKRRGDLEPQLGSPQQKPKRLRLVVDVSGSMYRFNGVDRRLERSMEAVCMVMEAFENYEEKFKYDIAGHSGDGYNIKLVPVNQIPKNNKQRLEILKTMHEHSQFCMSGDHTLEGTEHAIKDITTEEADEYFVIILSDANLSRYGINPARFAQILTSDPQVNAFAIFIGSLGDQAARLQRTLPAGRSFIAMDTKKIPQILQQIFTSTMLSSI

Target ORF information:

RefSeq Version	NM_027906.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus von Willebrand factor A domain containing 8 (Vwa8), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027906.1

ORF Insert Sequence:

ATGCAGTCAC GCCTCCTCCT CCTAGGAGCC CCGGGCGGCC TTGGGGACGT GGCCTCCCGG 
CGAGTGCGGC TGCTCTTGCG GCAGGTGTTG CGGGGCAGGC CGGGCGGGGA CCAGCAGCGG 
CTGGAGGTCA GGCTGCTGCA CTCTGGGGCG ACCGACTCAG GTGAAACGGT TAGTATTGGA 
GATGTGTCCT ACAAGCTGAA AACTCCCAAG AATCCAGAAC TTGTCCCACA GAACTACATT 
TCAGACTCTC CAGCTCAGTC CATAGTCCAG CATCTGAGAT GGCTGATGCA GAAGGATCTG 
CTGGGGCAGG ATGTCTTTCT CATCGGACCT CCTGGGCCTC TCCGGCGCTC GGTGGCTATG 
CAGTACTTGG AGCTGACCAA ACGAGAGGTG GAATACATCG CCCTGTCAAG GGACACCACT 
GAAACTGACC TCAAACAGCG CCGGGAGATC CGAGCTGGCA CAGCCTTTTA CATTGACCAG 
TGTGCGGTTC GGGCTGCCAC AGAAGGCAGG ACGCTGGTTT TAGAAGGCTT GGAAAAGGCG 
GAGAGAAACG TGCTTCCAGT ACTGAACAAC TTGCTGGAGA ACCGGGAGAT GCAGCTTGAA 
GATGGGCGCT TCCTCATGTC TGCGGAGCGC TATGACAAGC TTCTCCAAGA TCACACTAAA 
GAGGAGCTGG ACGCGTGGAA GATTGTCCGA GTCAGTGAGA ATTTCCGAGT GATTGCCTTG 
GGCTTGCCAG TGCCCAGGTA CTCTGGGAAT CCATTAGACC CCCCTCTCCG GTCTCGATTT 
CAAGCCAGAG ATATCTATTT TCTACCCTTT CAGGACCAAC TAAAACTATT GTATTCAGTT 
GGAGCCAATG TTTCTGCTGA GAAGATTTCT CAGCTCTTGT CCTTTGCGAC AACCCTCTGC 
TCCCAAGAAT CCTCTACGCT TGGGCTTCCA GACTTTCCCT TAGATAGTTT GCCAGAGGCA 
GTTCAAATCC TGGACTCCTT TCCCATGATG TCGATTGAAC ATGCACTCCA GTGGGTGTAT 
CCTTACACTC TTTTACTCGG ACACGAGGGG AAGATGGCCG TGGAGGGTGT TTTAAAACGC 
TTTGAGCTCC AAGGTTCTGG ACATTCCCTG CTTCCTAAGG AGATTGTAAG AGTGGAGAGG 
ATGACTGACA GCCACGGCTC CTATGCCCAC GTGACAATCC GGGTCGCAGG AAAAGAGGTG 
ACCATTAAGG TACCAGCGGG GACCAGAGCA GTAAACCAGC CTTGTGCTCC TGACCATTTC 
ATTCAGACTG TCAGTCACAA GCAATTACTG GCTGAGATGG TGCAGTCTCA TATGGTGAAG 
GACATATGCT TAATTGGAGG GAAGGGCTGT GGGAAAACGG TCATCGCTAA GAACTTTGCT 
GCTCTCCTAG GATACAGCAT AGAACCCATC ATGCTTTACC AGGATATGAC TGCGCGGGAC 
CTGCTACAGC AGCGGTACAC GCTCCCCAAC GGCGACACCG CCTGGCGCTC CTCACCCCTT 
GTGAGTGCTG CCCGGGAAGG CAAACTGGTC CTGTTGGATG GTATCCACCG CGTCAACGCT 
GGCACACTGG CTGTATTGCA AAGACTGATC CATGACCGGG AGCTCAGCCT GTATGATGGA 
TCTCGGCTGC TGAGGGAGGA CAGGTACCTG AGCTTGAAGG AGAAGCTACA ACTGACCGAT 
GAGCAGCTAC AGAACAGGTC CATTTTTCCT ATCCACCCTT CCTTCAGAAT CATTGCGTTG 
GCAGAGCCCC CAATTGTTGG AAGCACAACA CAGCAGTGGC TGGGACCAGA ATTTTTAACT 
ATGTTCTTTT TCCATCACAT GAAGCCTCTT GTGAAAAGTG AAGAAATCCA AGTGATAAAG 
GAGACGGTTC CAAATGTACC TCAGGAAGCA CTGGAGAAAT TGCTGTCAGT CACTCACAAA 
CTCAGGGAGA CCCAGGATCC CACAGCACAG TCCTTGGCAG CCTCGCTTTC CACCCGTCAG 
CTCTTACGGA TCTCTCGCCG GCTCTCGAAG TATCCCAGCG AAAATCTTCA TGATGCCATT 
ACCAAAGCCT GTCTTTCCAG GTTCTTACCA AGCCTTGCTC AGTCTGCACT GGAGAAGAAT 
CTGGCAGATG CGGCAATAGA AACAAACACT GAGGACAGCC TGGAGCCAGA GCTGGAGAAT 
TACAAGTGTG AAGTAGTCGC CGGGTCTCTG AAGATTGGCG CCGTCAGTGT GCCAGTGCAC 
AATGCCCATG AGAAGATGAA AGTGCCTGAC GTTCTTTTCT ACGACAATGT TCAGCACATG 
GTAGTGATGG AAGACATGCT TAAAGACTTT GTCCTTGGCG AACACTTACT ATTGGTTGGT 
AACCAGGGGG TAGGAAAGAA CAAGATTGTG GACCGATTCC TTCACCTGCT CAACAGACCC 
CGAGAATATA TACAGCTGCA CAGGGACACC ACAGTGCAGT CTCTGACTCT CCAGCCCACA 
GTGAAAGGTG GACTGATTGT ATATGAAGAC TCACCTCTGG TTAAAGCAGT AAAGTTGGGT 
CATATTCTAG TAGTAGATGA GGCAGACAAA GCCCCAACAA ATGTCACCTG TATTTTGAAG 
ACTCTGGTAG AAAACGGAGA AATGATTCTT GCGGACGGAA GACGTATTGT TGCAGATGCA 
GCAAATGTGG ATGGAAGAGA GAACCTTGTC GCGATCCATC CTGACTTCAG GATGCTGGCT 
CTGGCCAACA GGCCTGGCTT CCCCTTCTTG GGCAATGATT TCTTTGGAAC TTTAGGTGAC 
ATTTTTAGCT GTCATGCCAT TGACAACCCC AAGCCCCACT CAGAGCTTTC GATGCTCAAA 
CAGTACGGCC CAGACGTGCC TGAGCCCGTC CTGCAGAAGC TCGTGGCAGC CTTTGGAGAG 
CTGAGGAACT TGGCTGACCA AGGGATTATC AACTACCCTT ATTCCACCAG AGAAGTGGTC 
AACATCGTCA AGCACTTACA GAAATTTCCC ACTGAAGGCC TCTCCAGTGT GGTTCGGAAT 
GTATTTGACT TCGATTCTTA CAACAACGAC ATGAGGGAGA TTTTGATGAA CACTTTGCAC 
AAATATGGGA TCCCCATTGG AGCAAAGCCT ACCAACGTGC AGCTGGCAAA GGAGTTTCCT 
CTGCCAGAGA AGACATTCAT GGGCTACTGG ATAGTTGGCC AGACGGGAAA TGGAATGCAG 
AAGGTCTTGT GTCCAGCTGA AACAAATCAC GTTGACATAA AGGGCCCAGT ACTTGTGAAC 
ATGGAAAAGT ACCCAATAGA AAAACATGAA GCGAGATTCC TAAGCTTTAC TGAAGAGTGT 
ACGTCCTGGA AGTTTCCATT GGATGAAGTC AATTTAATCT GTGACATCGC TGTATCACAT 
GAAAACGGAG AACAGACTCT GTATGTGGCT GCGTGCAACC CGGTTTCCTT GTACTTCATG 
AATATGACAG GGAAAAATGG CTTCTTTGTG GACTTCTTCG ACATCTTCCC AAGGATGGCC 
AGTGGGTCCT GGCGACCATT TGTAACAGTA GCCCCACTGG GAAGTCCTCT GAGGGGTCAA 
GTTGTTCTGC ATGAGGAGCA GAGTAATGCC GTCCTCTTAC TCGACACTAC TGGCAGTGCC 
ATTCGCCGTC TCGTCCTTCC CACAGAAGAG TTTACATCTA AGAAGTCTTC ATGGTGGAGC 
AAGGAGGAAG GTGAAACTTA TAGAATGTGT AAAGAATTCT CACACAAAAA CTGGGTAGTA 
TTCTACAAAC AAACAGGGAA TAGCTTGACT GTCCTGGATG TCTTAGAAGG ACTAGCTCAC 
ACCATCTCAC TTCCCATCAA CCTCAGGACA GTTTTCCTGG TGGCGGAGGA CAAATGGCTT 
CTTGTGGAAA ACGAAACAAA TCAGAAGTAT CTTTTGACTA AGCCTGCACA CATTGGTTCT 
GAGGACACTG GGGCCTGTCA GTTGTATATG TTGAAAGAAG AGCTGCCTAG CACAGGATTT 
GGAGTCACAC AAGAAACAGA GTTCTGCATA CCTGATAAAG TGTCAAGTGA TCAGCTGTCA 
TCTGAAAACC TAACTTCAGC TGTGGGCCAG AAGATCGCCT CTCCTAACCG TATTCTCTCG 
GATGAAAATA GCCACGCTAC CATAGTTGTT GGCTTCCCGG ACCTCATGTC ACCCAGTGAA 
GTTTATTCAT GGAAGAGGTC TTCGTCTTTG AGACAACCAA GTGTTACCAG CATGACAATG 
TACACAGGGA AGCGGACAAA TGCCACCCCA CGGCACAACA ACTGCGTGAC TCTCACACAT 
ACTAATCAGG TGGTGAGGAT TCTGCCCCCA GGAGAAGTCC CTCTGAAAGA CCTCTACCCA 
AAAGATGTTA CTCCCCCCCA GACAGCTGGC TACATAGAGG TTACTGATCT CCAAGCAAAG 
AAACTCCGTT ACATCCCTGT CCCCAGCGCA GAGTCTCTCT CGCCATACAC CGCATGGATA 
TCTGCTATTT CAGACACAGA TGCTCTGCTG GCTGAGTGGG ACAAGAGCAG CGTCGTGACT 
GTTGATATGG GAGGGCGTGT CAGGCTCTGG GAAACTGGAC TTGAACGTCT GCAGCAGTCG 
CTCATGGAAT GGAGAAACAT GATTGGACAA GACAGTGACA AACATGTGCA GATAACAATC 
GAAAGAGACA GTAATGAAGA CGTGAGTGAC CCCAAACATG GCAAGGAGGA CCCAGACAAC 
ATGCCCCATG TGGGTGGCAA TACCTGGGCT GGTGGAACAG GGGGAAGAGA CACCGCAGGT 
CTTGGTGGCA AAGGAGGTCC TTATCGCTTG GATGCAGGCC ACCCAGTGTA TCAAGTCTCT 
GAGGTGGAGA AAGATGCAGT TCCTGAGGAT GTCAAGAGAG CTGCAAGAGA GATGGCCCAG 
AAAGCATTTC AGCAGAGACT AAAGGAGATC CAGATGAGCG AGTACGACGC CGCAACCTAC 
GAGCGGTTTT CAAGTGCTGT CCAGCGGCAG GTGCATGCTC TCCGCATCAT CCTGGATAAT 
CTACAGGCTA AAGGCAAAGA AAGACAGTGG CTAAGACACC AAGCTACCGG AGAACTGGAC 
GACGCCAAGA TCATTGACGG GCTGGCGGGA GAAAAGTCCA TCTACAAACG TCGGGGTGAC 
CTGGAACCCC AGCTGGGTAG TCCACAACAG AAGCCCAAGC GCCTGCGCCT GGTGGTGGAC 
GTCTCCGGCA GCATGTACCG CTTCAACGGG GTGGATCGCC GGCTAGAGCG CTCGATGGAG 
GCAGTGTGCA TGGTCATGGA AGCCTTCGAG AACTACGAGG AGAAATTCAA GTACGACATC 
GCTGGACACT CGGGAGATGG CTACAACATT AAGCTCGTTC CAGTTAACCA AATCCCCAAG 
AACAATAAGC AGAGACTAGA AATTCTGAAG ACGATGCACG AGCACTCTCA GTTCTGCATG 
AGTGGGGACC ACACACTTGA GGGAACGGAA CACGCCATCA AGGACATTAC CACAGAAGAA 
GCTGATGAGT ACTTTGTCAT CATCTTAAGT GATGCAAATC TGTCCCGATA CGGAATAAAC 
CCGGCCCGCT TTGCCCAGAT CCTGACAAGC GATCCTCAAG TAAACGCCTT TGCCATTTTT 
ATCGGCTCTT TGGGGGATCA GGCAGCCAGG CTTCAGAGAA CTCTGCCAGC TGGCCGGTCC 
TTCATCGCTA TGGATACCAA GAAGATCCCT CAGATCTTAC AACAGATCTT CACCTCCACC 
ATGCTGTCCA GTATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vwa8 ( XM_006518904.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006518967.1 Mus musculus von Willebrand factor A domain containing 8 (Vwa8), transcript variant X2, mRNA.

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CloneID	OMu49915
Clone ID Related Accession (Same CDS sequence)	XM_006518904.3
Accession Version	XM_006518904.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	von Willebrand factor A domain-containing protein 8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006518904.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCAC GCCTCCTCCT CCTAGGAGCC CCGGGCGGCC TTGGGGACGT GGCCTCCCGG 
CGAGTGCGGC TGCTCTTGCG GCAGGTGTTG CGGGGCAGGC CGGGCGGGGA CCAGCAGCGG 
CTGGAGGTCA GGCTGCTGCA CTCTGGGGCG ACCGACTCAG GTGAAACGGT TAGTATTGGA 
GATGTGTCCT ACAAGCTGAA AACTCCCAAG AATCCAGAAC TTGTCCCACA GAACTACATT 
TCAGACTCTC CAGCTCAGTC CATAGTCCAG CATCTGAGAT GGCTGATGCA GAAGGATCTG 
CTGGGGCAGG ATGTCTTTCT CATCGGACCT CCTGGGCCTC TCCGGCGCTC GGTGGCTATG 
CAGTACTTGG AGCTGACCAA ACGAGAGGTG GAATACATCG CCCTGTCAAG GGACACCACT 
GAAACTGACC TCAAACAGCG CCGGGAGATC CGAGCTGGCA CAGCCTTTTA CATTGACCAG 
TGTGCGGTTC GGGCTGCCAC AGAAGGCAGG ACGCTGGTTT TAGAAGGCTT GGAAAAGGCG 
GAGAGAAACG TGCTTCCAGT ACTGAACAAC TTGCTGGAGA ACCGGGAGAT GCAGCTTGAA 
GATGGGCGCT TCCTCATGTC TGCGGAGCGC TATGACAAGC TTCTCCAAGA TCACACTAAA 
GAGGAGCTGG ACGCGTGGAA GATTGTCCGA GTCAGTGAGA ATTTCCGAGT GATTGCCTTG 
GGCTTGCCAG TGCCCAGGTA CTCTGGGAAT CCATTAGACC CCCCTCTCCG GTCTCGATTT 
CAAGCCAGAG ATATCTATTT TCTACCCTTT CAGGACCAAC TAAAACTATT GTATTCAGTT 
GGAGCCAATG TTTCTGCTGA GAAGATTTCT CAGCTCTTGT CCTTTGCGAC AACCCTCTGC 
TCCCAAGAAT CCTCTACGCT TGGGCTTCCA GACTTTCCCT TAGATAGTTT GCCAGAGGCA 
GTTCAAATCC TGGACTCCTT TCCCATGATG TCGATTGAAC ATGCACTCCA GTGGGTGTAT 
CCTTACACTC TTTTACTCGG ACACGAGGGG AAGATGGCCG TGGAGGGTGT TTTAAAACGC 
TTTGAGCTCC AAGGTTCTGG ACATTCCCTG CTTCCTAAGG AGATTGTAAG AGTGGAGAGG 
ATGACTGACA GCCACGGCTC CTATGCCCAC GTGACAATCC GGGTCGCAGG AAAAGAGGTG 
ACCATTAAGG TACCAGCGGG GACCAGAGCA GTAAACCAGC CTTGTGCTCC TGACCATTTC 
ATTCAGACTG TCAGTCACAA GCAATTACTG GCTGAGATGG TGCAGTCTCA TATGGTGAAG 
GACATATGCT TAATTGGAGG GAAGGGCTGT GGGAAAACGG TCATCGCTAA GAACTTTGCT 
GCTCTCCTAG GATACAGCAT AGAACCCATC ATGCTTTACC AGGATATGAC TGCGCGGGAC 
CTGCTACAGC AGCGGTACAC GCTCCCCAAC GGCGACACCG CCTGGCGCTC CTCACCCCTT 
GTGAGTGCTG CCCGGGAAGG CAAACTGGTC CTGTTGGATG GTATCCACCG CGTCAACGCT 
GGCACACTGG CTGTATTGCA AAGACTGATC CATGACCGGG AGCTCAGCCT GTATGATGGA 
TCTCGGCTGC TGAGGGAGGA CAGGTACCTG AGCTTGAAGG AGAAGCTACA ACTGACCGAT 
GAGCAGCTAC AGAACAGGTC CATTTTTCCT ATCCACCCTT CCTTCAGAAT CATTGCGTTG 
GCAGAGCCCC CAATTGTTGG AAGCACAACA CAGCAGTGGC TGGGACCAGA ATTTTTAACT 
ATGTTCTTTT TCCATCACAT GAAGCCTCTT GTGAAAAGTG AAGAAATCCA AGTGATAAAG 
GAGACGGTTC CAAATGTACC TCAGGAAGCA CTGGAGAAAT TGCTGTCAGT CACTCACAAA 
CTCAGGGAGA CCCAGGATCC CACAGCACAG TCCTTGGCAG CCTCGCTTTC CACCCGTCAG 
CTCTTACGGA TCTCTCGCCG GCTCTCGAAG TATCCCAGCG AAAATCTTCA TGATGCCATT 
ACCAAAGCCT GTCTTTCCAG GTTCTTACCA AGCCTTGCTC AGTCTGCACT GGAGAAGAAT 
CTGGCAGATG CGGCAATAGA AACAAACACT GAGGACAGCC TGGAGCCAGA GCTGGAGAAT 
TACAAGTGTG AAGTAGTCGC CGGGTCTCTG AAGATTGGCG CCGTCAGTGT GCCAGTGCAC 
AATGCCCATG AGAAGATGAA AGTGCCTGAC GTTCTTTTCT ACGACAATGT TCAGCACATG 
GTAGTGATGG AAGACATGCT TAAAGACTTT GTCCTTGGCG AACACTTACT ATTGGTTGGT 
AACCAGGGGG TAGGAAAGAA CAAGATTGTG GACCGATTCC TTCACCTGCT CAACAGACCC 
CGAGAATATA TACAGCTGCA CAGGGACACC ACAGTGCAGT CTCTGACTCT CCAGCCCACA 
GTGAAAGGTG GACTGATTGT ATATGAAGAC TCACCTCTGG TTAAAGCAGT AAAGTTGGGT 
CATATTCTAG TAGTAGATGA GGCAGACAAA GCCCCAACAA ATGTCACCTG TATTTTGAAG 
ACTCTGGTAG AAAACGGAGA AATGATTCTT GCGGACGGAA GACGTATTGT TGCAGATGCA 
GCAAATGTGG ATGGAAGAGA GAACCTTGTC GCGATCCATC CTGACTTCAG GATGCTGGCT 
CTGGCCAACA GGCCTGGCTT CCCCTTCTTG GGCAATGATT TCTTTGGAAC TTTAGGTGAC 
ATTTTTAGCT GTCATGCCAT TGACAACCCC AAGCCCCACT CAGAGCTTTC GATGCTCAAA 
CAGTACGGCC CAGACGTGCC TGAGCCCGTC CTGCAGAAGC TCGTGGCAGC CTTTGGAGAG 
CTGAGGAACT TGGCTGACCA AGGGATTATC AACTACCCTT ATTCCACCAG AGAAGTGGTC 
AACATCGTCA AGCACTTACA GAAATTTCCC ACTGAAGGCC TCTCCAGTGT GGTTCGGAAT 
GTATTTGACT TCGATTCTTA CAACAACGAC ATGAGGGAGA TTTTGATGAA CACTTTGCAC 
AAATATGGGA TCCCCATTGG AGCAAAGCCT ACCAACGTGC AGCTGGCAAA GGAGTTTCCT 
CTGCCAGAGA AGACATTCAT GGGCTACTGG ATAGTTGGCC AGACGGGAAA TGGAATGCAG 
AAGGTCTTGT GTCCAGCTGA AACAAATCAC GTTGACATAA AGGGCCCAGT ACTTGTGAAC 
ATGGAAAAGT ACCCAATAGA AAAACATGAA GCGAGATTCC TAAGCTTTAC TGAAGAGTGT 
ACGTCCTGGA AGTTTCCATT GGATGAAGTC AATTTAATCT GTGACATCGC TGTATCACAT 
GAAAACGGAG AACAGACTCT GTATGTGGCT GCGTGCAACC CGGTTTCCTT GTACTTCATG 
AATATGACAG GGAAAAATGG CTTCTTTGTG GACTTCTTCG ACATCTTCCC AAGGATGGCC 
AGTGGGTCCT GGCGACCATT TGTAACAGTA GCCCCACTGG GAAGTCCTCT GAGGGGTCAA 
GTTGTTCTGC ATGAGGAGCA GAGTAATGCC GTCCTCTTAC TCGACACTAC TGGCAGTGCC 
ATTCGCCGTC TCGTCCTTCC CACAGAAGAG TTTACATCTA AGAAGTCTTC ATGGTGGAGC 
AAGGAGGAAG GTGAAACTTA TAGAATGTGT AAAGAATTCT CACACAAAAA CTGGGTAGTA 
TTCTACAAAC AAACAGGGAA TAGCTTGACT GTCCTGGATG TCTTAGAAGG ACTAGCTCAC 
ACCATCTCAC TTCCCATCAA CCTCAGGACA GTTTTCCTGG TGGCGGAGGA CAAATGGCTT 
CTTGTGGAAA ACGAAACAAA TCAGAAGTAT CTTTTGACTA AGCCTGCACA CATTGGTTCT 
GAGGACACTG GGGCCTGTCA GTTGTATATG TTGAAAGAAG AGCTGCCTAG CACAGGATTT 
GGAGTCACAC AAGAAACAGA GTTCTGCATA CCTGATAAAG TGTCAAGTGA TCAGCTGTCA 
TCTGAAAACC TAACTTCAGC TGTGGGCCAG AAGATCGCCT CTCCTAACCG TATTCTCTCG 
GATGAAAATA GCCACGCTAC CATAGTTGTT GGCTTCCCGG ACCTCATGAG TGCTGTCGCA 
GCACGGTGTC TGACGCAGCC TGGATGGAAC GATGGAGCCG CACTGATGAG CTCGCTGCCG 
CCCTTGCTGC TGAGTCACCC AGTGAAGTTT ATTCATGGAA GAGGTCTTCG TCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006518967.1
CDS	229..4485
Translation	MQSRLLLLGAPGGLGDVASRRVRLLLRQVLRGRPGGDQQRLEVRLLHSGATDSGETVSIGDVSYKLKTPKNPELVPQNYISDSPAQSIVQHLRWLMQKDLLGQDVFLIGPPGPLRRSVAMQYLELTKREVEYIALSRDTTETDLKQRREIRAGTAFYIDQCAVRAATEGRTLVLEGLEKAERNVLPVLNNLLENREMQLEDGRFLMSAERYDKLLQDHTKEELDAWKIVRVSENFRVIALGLPVPRYSGNPLDPPLRSRFQARDIYFLPFQDQLKLLYSVGANVSAEKISQLLSFATTLCSQESSTLGLPDFPLDSLPEAVQILDSFPMMSIEHALQWVYPYTLLLGHEGKMAVEGVLKRFELQGSGHSLLPKEIVRVERMTDSHGSYAHVTIRVAGKEVTIKVPAGTRAVNQPCAPDHFIQTVSHKQLLAEMVQSHMVKDICLIGGKGCGKTVIAKNFAALLGYSIEPIMLYQDMTARDLLQQRYTLPNGDTAWRSSPLVSAAREGKLVLLDGIHRVNAGTLAVLQRLIHDRELSLYDGSRLLREDRYLSLKEKLQLTDEQLQNRSIFPIHPSFRIIALAEPPIVGSTTQQWLGPEFLTMFFFHHMKPLVKSEEIQVIKETVPNVPQEALEKLLSVTHKLRETQDPTAQSLAASLSTRQLLRISRRLSKYPSENLHDAITKACLSRFLPSLAQSALEKNLADAAIETNTEDSLEPELENYKCEVVAGSLKIGAVSVPVHNAHEKMKVPDVLFYDNVQHMVVMEDMLKDFVLGEHLLLVGNQGVGKNKIVDRFLHLLNRPREYIQLHRDTTVQSLTLQPTVKGGLIVYEDSPLVKAVKLGHILVVDEADKAPTNVTCILKTLVENGEMILADGRRIVADAANVDGRENLVAIHPDFRMLALANRPGFPFLGNDFFGTLGDIFSCHAIDNPKPHSELSMLKQYGPDVPEPVLQKLVAAFGELRNLADQGIINYPYSTREVVNIVKHLQKFPTEGLSSVVRNVFDFDSYNNDMREILMNTLHKYGIPIGAKPTNVQLAKEFPLPEKTFMGYWIVGQTGNGMQKVLCPAETNHVDIKGPVLVNMEKYPIEKHEARFLSFTEECTSWKFPLDEVNLICDIAVSHENGEQTLYVAACNPVSLYFMNMTGKNGFFVDFFDIFPRMASGSWRPFVTVAPLGSPLRGQVVLHEEQSNAVLLLDTTGSAIRRLVLPTEEFTSKKSSWWSKEEGETYRMCKEFSHKNWVVFYKQTGNSLTVLDVLEGLAHTISLPINLRTVFLVAEDKWLLVENETNQKYLLTKPAHIGSEDTGACQLYMLKEELPSTGFGVTQETEFCIPDKVSSDQLSSENLTSAVGQKIASPNRILSDENSHATIVVGFPDLMSAVAARCLTQPGWNDGAALMSSLPPLLLSHPVKFIHGRGLRL

Target ORF information:

RefSeq Version	XM_006518904.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus von Willebrand factor A domain containing 8 (Vwa8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006518904.3

ORF Insert Sequence:

ATGCAGTCAC GCCTCCTCCT CCTAGGAGCC CCGGGCGGCC TTGGGGACGT GGCCTCCCGG 
CGAGTGCGGC TGCTCTTGCG GCAGGTGTTG CGGGGCAGGC CGGGCGGGGA CCAGCAGCGG 
CTGGAGGTCA GGCTGCTGCA CTCTGGGGCG ACCGACTCAG GTGAAACGGT TAGTATTGGA 
GATGTGTCCT ACAAGCTGAA AACTCCCAAG AATCCAGAAC TTGTCCCACA GAACTACATT 
TCAGACTCTC CAGCTCAGTC CATAGTCCAG CATCTGAGAT GGCTGATGCA GAAGGATCTG 
CTGGGGCAGG ATGTCTTTCT CATCGGACCT CCTGGGCCTC TCCGGCGCTC GGTGGCTATG 
CAGTACTTGG AGCTGACCAA ACGAGAGGTG GAATACATCG CCCTGTCAAG GGACACCACT 
GAAACTGACC TCAAACAGCG CCGGGAGATC CGAGCTGGCA CAGCCTTTTA CATTGACCAG 
TGTGCGGTTC GGGCTGCCAC AGAAGGCAGG ACGCTGGTTT TAGAAGGCTT GGAAAAGGCG 
GAGAGAAACG TGCTTCCAGT ACTGAACAAC TTGCTGGAGA ACCGGGAGAT GCAGCTTGAA 
GATGGGCGCT TCCTCATGTC TGCGGAGCGC TATGACAAGC TTCTCCAAGA TCACACTAAA 
GAGGAGCTGG ACGCGTGGAA GATTGTCCGA GTCAGTGAGA ATTTCCGAGT GATTGCCTTG 
GGCTTGCCAG TGCCCAGGTA CTCTGGGAAT CCATTAGACC CCCCTCTCCG GTCTCGATTT 
CAAGCCAGAG ATATCTATTT TCTACCCTTT CAGGACCAAC TAAAACTATT GTATTCAGTT 
GGAGCCAATG TTTCTGCTGA GAAGATTTCT CAGCTCTTGT CCTTTGCGAC AACCCTCTGC 
TCCCAAGAAT CCTCTACGCT TGGGCTTCCA GACTTTCCCT TAGATAGTTT GCCAGAGGCA 
GTTCAAATCC TGGACTCCTT TCCCATGATG TCGATTGAAC ATGCACTCCA GTGGGTGTAT 
CCTTACACTC TTTTACTCGG ACACGAGGGG AAGATGGCCG TGGAGGGTGT TTTAAAACGC 
TTTGAGCTCC AAGGTTCTGG ACATTCCCTG CTTCCTAAGG AGATTGTAAG AGTGGAGAGG 
ATGACTGACA GCCACGGCTC CTATGCCCAC GTGACAATCC GGGTCGCAGG AAAAGAGGTG 
ACCATTAAGG TACCAGCGGG GACCAGAGCA GTAAACCAGC CTTGTGCTCC TGACCATTTC 
ATTCAGACTG TCAGTCACAA GCAATTACTG GCTGAGATGG TGCAGTCTCA TATGGTGAAG 
GACATATGCT TAATTGGAGG GAAGGGCTGT GGGAAAACGG TCATCGCTAA GAACTTTGCT 
GCTCTCCTAG GATACAGCAT AGAACCCATC ATGCTTTACC AGGATATGAC TGCGCGGGAC 
CTGCTACAGC AGCGGTACAC GCTCCCCAAC GGCGACACCG CCTGGCGCTC CTCACCCCTT 
GTGAGTGCTG CCCGGGAAGG CAAACTGGTC CTGTTGGATG GTATCCACCG CGTCAACGCT 
GGCACACTGG CTGTATTGCA AAGACTGATC CATGACCGGG AGCTCAGCCT GTATGATGGA 
TCTCGGCTGC TGAGGGAGGA CAGGTACCTG AGCTTGAAGG AGAAGCTACA ACTGACCGAT 
GAGCAGCTAC AGAACAGGTC CATTTTTCCT ATCCACCCTT CCTTCAGAAT CATTGCGTTG 
GCAGAGCCCC CAATTGTTGG AAGCACAACA CAGCAGTGGC TGGGACCAGA ATTTTTAACT 
ATGTTCTTTT TCCATCACAT GAAGCCTCTT GTGAAAAGTG AAGAAATCCA AGTGATAAAG 
GAGACGGTTC CAAATGTACC TCAGGAAGCA CTGGAGAAAT TGCTGTCAGT CACTCACAAA 
CTCAGGGAGA CCCAGGATCC CACAGCACAG TCCTTGGCAG CCTCGCTTTC CACCCGTCAG 
CTCTTACGGA TCTCTCGCCG GCTCTCGAAG TATCCCAGCG AAAATCTTCA TGATGCCATT 
ACCAAAGCCT GTCTTTCCAG GTTCTTACCA AGCCTTGCTC AGTCTGCACT GGAGAAGAAT 
CTGGCAGATG CGGCAATAGA AACAAACACT GAGGACAGCC TGGAGCCAGA GCTGGAGAAT 
TACAAGTGTG AAGTAGTCGC CGGGTCTCTG AAGATTGGCG CCGTCAGTGT GCCAGTGCAC 
AATGCCCATG AGAAGATGAA AGTGCCTGAC GTTCTTTTCT ACGACAATGT TCAGCACATG 
GTAGTGATGG AAGACATGCT TAAAGACTTT GTCCTTGGCG AACACTTACT ATTGGTTGGT 
AACCAGGGGG TAGGAAAGAA CAAGATTGTG GACCGATTCC TTCACCTGCT CAACAGACCC 
CGAGAATATA TACAGCTGCA CAGGGACACC ACAGTGCAGT CTCTGACTCT CCAGCCCACA 
GTGAAAGGTG GACTGATTGT ATATGAAGAC TCACCTCTGG TTAAAGCAGT AAAGTTGGGT 
CATATTCTAG TAGTAGATGA GGCAGACAAA GCCCCAACAA ATGTCACCTG TATTTTGAAG 
ACTCTGGTAG AAAACGGAGA AATGATTCTT GCGGACGGAA GACGTATTGT TGCAGATGCA 
GCAAATGTGG ATGGAAGAGA GAACCTTGTC GCGATCCATC CTGACTTCAG GATGCTGGCT 
CTGGCCAACA GGCCTGGCTT CCCCTTCTTG GGCAATGATT TCTTTGGAAC TTTAGGTGAC 
ATTTTTAGCT GTCATGCCAT TGACAACCCC AAGCCCCACT CAGAGCTTTC GATGCTCAAA 
CAGTACGGCC CAGACGTGCC TGAGCCCGTC CTGCAGAAGC TCGTGGCAGC CTTTGGAGAG 
CTGAGGAACT TGGCTGACCA AGGGATTATC AACTACCCTT ATTCCACCAG AGAAGTGGTC 
AACATCGTCA AGCACTTACA GAAATTTCCC ACTGAAGGCC TCTCCAGTGT GGTTCGGAAT 
GTATTTGACT TCGATTCTTA CAACAACGAC ATGAGGGAGA TTTTGATGAA CACTTTGCAC 
AAATATGGGA TCCCCATTGG AGCAAAGCCT ACCAACGTGC AGCTGGCAAA GGAGTTTCCT 
CTGCCAGAGA AGACATTCAT GGGCTACTGG ATAGTTGGCC AGACGGGAAA TGGAATGCAG 
AAGGTCTTGT GTCCAGCTGA AACAAATCAC GTTGACATAA AGGGCCCAGT ACTTGTGAAC 
ATGGAAAAGT ACCCAATAGA AAAACATGAA GCGAGATTCC TAAGCTTTAC TGAAGAGTGT 
ACGTCCTGGA AGTTTCCATT GGATGAAGTC AATTTAATCT GTGACATCGC TGTATCACAT 
GAAAACGGAG AACAGACTCT GTATGTGGCT GCGTGCAACC CGGTTTCCTT GTACTTCATG 
AATATGACAG GGAAAAATGG CTTCTTTGTG GACTTCTTCG ACATCTTCCC AAGGATGGCC 
AGTGGGTCCT GGCGACCATT TGTAACAGTA GCCCCACTGG GAAGTCCTCT GAGGGGTCAA 
GTTGTTCTGC ATGAGGAGCA GAGTAATGCC GTCCTCTTAC TCGACACTAC TGGCAGTGCC 
ATTCGCCGTC TCGTCCTTCC CACAGAAGAG TTTACATCTA AGAAGTCTTC ATGGTGGAGC 
AAGGAGGAAG GTGAAACTTA TAGAATGTGT AAAGAATTCT CACACAAAAA CTGGGTAGTA 
TTCTACAAAC AAACAGGGAA TAGCTTGACT GTCCTGGATG TCTTAGAAGG ACTAGCTCAC 
ACCATCTCAC TTCCCATCAA CCTCAGGACA GTTTTCCTGG TGGCGGAGGA CAAATGGCTT 
CTTGTGGAAA ACGAAACAAA TCAGAAGTAT CTTTTGACTA AGCCTGCACA CATTGGTTCT 
GAGGACACTG GGGCCTGTCA GTTGTATATG TTGAAAGAAG AGCTGCCTAG CACAGGATTT 
GGAGTCACAC AAGAAACAGA GTTCTGCATA CCTGATAAAG TGTCAAGTGA TCAGCTGTCA 
TCTGAAAACC TAACTTCAGC TGTGGGCCAG AAGATCGCCT CTCCTAACCG TATTCTCTCG 
GATGAAAATA GCCACGCTAC CATAGTTGTT GGCTTCCCGG ACCTCATGAG TGCTGTCGCA 
GCACGGTGTC TGACGCAGCC TGGATGGAAC GATGGAGCCG CACTGATGAG CTCGCTGCCG 
CCCTTGCTGC TGAGTCACCC AGTGAAGTTT ATTCATGGAA GAGGTCTTCG TCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Characterization of the novel protein KIAA0564 (Von Willebrand Domain-containing Protein 8).
Biochemical and biophysical research communications487(3)545-551(2017 06)
Luo M,Mengos AE,Ma W,Finlayson J,Bustos RZ,Xiao Zhu Y,Shi CX,Stubblefield TM,Willis WT,Mandarino LJ

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Integrating genetic and network analysis to characterize genes related to mouse weight.
PLoS genetics2(8)e130(2006 Aug)
Ghazalpour A,Doss S,Zhang B,Wang S,Plaisier C,Castellanos R,Brozell A,Schadt EE,Drake TA,Lusis AJ,Horvath S

Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.
Cell115(5)629-40(2003 Nov)
Mootha VK,Bunkenborg J,Olsen JV,Hjerrild M,Wisniewski JR,Stahl E,Bolouri MS,Ray HN,Sihag S,Kamal M,Patterson N,Lander ES,Mann M

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Vwa8 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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