Wapl cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Wapl
Entrez Gene ID	218914
Full Name	WAPL cohesin release factor
Synonyms	A530089A20Rik,BC037674,DIF-2,FOE,Wapal
General protein information	Preferred Names WAPL cohesin release factor Names wings apart-like protein homolog dioxin-inducible factor 2 friend of EBNA2 wings apart-like homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 14 Map Location: 14\|14 B
Summary	Studies suggest that the protein encoded by this gene is important for the release of cohesin from chromatin. This gene product is thought to be essential for development, and reduced expression of this gene in cells causes defects in chromatin structure. High levels of expression of the human ortholog of this gene are observed in cervical cancers, and expression of the human ortholog of this gene in mice results in tumor formation. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Aug 2014].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006518824.3	XP_006518887.1	wings apart-like protein homolog isoform X1
NM_001301330.1	NP_001288259.1	wings apart-like protein homolog isoform 2
XM_006518824.4	XP_006518887.1	wings apart-like protein homolog isoform X1
NM_001004436.4	NP_001004436.2	wings apart-like protein homolog isoform 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	WAPAL	NP_055860.1
Pan troglodytes (chimpanzee)	WAPAL	XP_003312721.1
	WAPAL	XP_002805691.1
Canis lupus familiaris (dog)	WAPAL	XP_864834.3
Mus musculus (house mouse)	Wapal	NP_001004436.2
Gallus gallus (chicken)	WAPAL	NP_001186440.1
Danio rerio (zebrafish)	wapala	XP_688605.5
Xenopus tropicalis (tropical clawed frog)	wapal	XP_002937603.2
Bos taurus (cattle)	WAPAL	XP_005226580.1
Caenorhabditis elegans (roundworm)	wapl-1	NP_500567.1
Rattus norvegicus (Norway rat)	Wapal	XP_006252785.1

Gene Ontology
Bibliography

Related articles in PubMed

A mechanism of cohesin-dependent loop extrusion organizes zygotic genome architecture.
Gassler J, Brand?o HB, Imakaev M, Flyamer IM, Ladst?tter S, Bickmore WA, Peters JM, Mirny LA, Tachibana K
The EMBO journal36(24)3600-3618(2017 12)

Cohesin is positioned in mammalian genomes by transcription, CTCF and Wapl.
Busslinger GA, Stocsits RR, van der Lelij P, Axelsson E, Tedeschi A, Galjart N, Peters JM
Nature544(7651)503-507(2017 04)

Cohesin Removal along the Chromosome Arms during the First Meiotic Division Depends on a NEK1-PP1?-WAPL Axis in the Mouse.
Brie?o-Enr?quez MA, Moak SL, Toledo M, Filter JJ, Gray S, Barbero JL, Cohen PE, Holloway JK
Cell reports17(4)977-986(2016 10)

Allogeneic T cell responses are regulated by a specific miRNA-mRNA network.
Sun Y, Tawara I, Zhao M, Qin ZS, Toubai T, Mathewson N, Tamaki H, Nieves E, Chinnaiyan AM, Reddy P
The Journal of clinical investigation123(11)4739-54(2013 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results demonstrate that the zygotic genome folds into loops and domains that critically depend on Scc1-cohesin and that are regulated in size and linear density by Wapl.
Title: A mechanism of cohesin-dependent loop extrusion organizes zygotic genome architecture.

distribution of cohesin in the mouse genome depends on transcription, CTCF and the cohesin release factor Wings apart-like (Wapl)
Title: Cohesin is positioned in mammalian genomes by transcription, CTCF and Wapl.

NEK1 phosphorylates PP1gamma, leading to the dephosphorylation of WAPL, which, in turn, results in its retention on chromosome cores to promote loss of cohesion at the end of prophase I in mammals.
Title: Cohesin Removal along the Chromosome Arms during the First Meiotic Division Depends on a NEK1-PP1?-WAPL Axis in the Mouse.

in mitosis, Wapl-mediated release of cohesin from DNA is essential for proper chromosome segregation and protects cohesin from cleavage by the protease separase, thus enabling mitotic exit in the presence of functional cohesin complexes
Title: Wapl is an essential regulator of chromatin structure and chromosome segregation.

mWAPL may be involved in spermatogenesis and be target genes mediating the reproductive toxicity induced by 2,3,7,8-trachlorodibenzo-p-dioxin .
Title: A dioxin sensitive gene, mammalian WAPL, is implicated in spermatogenesis.

The following Wapl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Wapl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu49859	XM_006518824.4 Latest version!	Mus musculus WAPL cohesin release factor (Wapl), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu10653	NM_001004436.4 Latest version!	Mus musculus WAPL cohesin release factor (Wapl), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu73083	NM_001301330.1 Latest version!	Mus musculus WAPL cohesin release factor (Wapl), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
View Old Accession Versions >>
OMu49859	XM_006518824.3	Mus musculus WAPL cohesin release factor (Wapl), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Wapl ( XM_006518824.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006518887.1 Mus musculus WAPL cohesin release factor (Wapl), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu49859
Clone ID Related Accession (Same CDS sequence)	XM_006518824.3 , XM_006518824.4
Accession Version	XM_006518824.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	wings apart-like protein homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006518824.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACATCCA GATTTGGAAA AACTTACAGT AGGAAAGGAG GAAATGGCAG TTCAAAATTT 
GATGAAGTTT TTTCCAACAA ACGGACTACT CTTAGTACAA AATGGGGTGA GACCACATTT 
ATGGCTAAAT TAGGGCAGAA GAGGCCCAAT TTCAAACCAG ATATTCAAGA AATTCCGAAG 
AAACCTAAAG TAGAAGAAGA AGATACTGGA GATCCCTTTG GTTTTGATAG TGATGATGAG 
TCTCTACCTG TTTCTTCAAA AAATTTAGCC CAGGGTAAGG GTTCATCTTA CTCAGAATCT 
AGTGAGGCTG CTCAGCTGGA AGAAGTCACT TCTGTATTTG AAGCTAATAG CAAATGTAGT 
CATGTGGTGG GTGAAGACAG TTTTGCTTCC GACAGATGCT TACTTGTGGA GGATACTTTA 
ATTGGGAAAG AGAAGAGCAT AAGTAGAATT CCAGAAGACA ACGCAAACAA AAGTAGTTGC 
ACTAAGTTGC TAACTTCAGA TAAAGTGGAG AATTTTAGTG AAGAACATGA AAAAAATAGT 
CACCACTTTC ACAAAAATGC TGAAGATAGT ACTAAGAAAC CCAATGCAGA AACCGCAGTG 
GCTTCTGAAT ATAAAGCTGA TGAAACTAAA GAAACAAATG ATACTTGGAA CTCCCAGTCT 
GGAAAAAGAA CAGAGTCTCC ATCTGAAAGT TGTCCAGTCA AAGGATCTGT AAGAACTGGT 
TTATATGAAT GGGATAATGA TTTTGAAGAT ATCAGGTCAG AAGACTGTAT TTTAAGTTTG 
GATAATGAGT CTCTTTTGGA GATGAAAGAC GAGGATTTAA AAAATCGGAT TGGAGGATTG 
GAAAATCTAA ATGAAACCTT TGAAGAAGAT ATCATACAAA GTGTTCTTAG GCCAAGCAAC 
TGTAGGACGT ACTGTAGGGC CAATAAAGCG AGATCCTCAC AGGGAGCATC AAATTTTGAT 
AAGCTAATGG ATGGCACCAG TCAGTCCTTA GCCAAAGCAA ACAGTGAATC AAGTAAAGAT 
GGCCTGAATC AGGCAAAGAA AGGTAGTGCA AGTTGTGGGA CCAGTTTTCG AGGAACAGTT 
GGACGGACTA GAGATTACAC TGTTTTACAT CCATCTTGCT TGTCAGTGTG TAATGTTACC 
ATCCAGGATA CTATGGAACG GAGTATGGAT GAGTTCACCG CATCCACTCC TGCAGATTTA 
GGAGAGGCTG GCCGGCTCAG AAAAAAGGCA GATATTGCAA CCTCCAAGAC CACTACTAGA 
TTTCGACCTA GTAATACTAA ATCCAAAAAG GATGTTAAAC TTGAATTTTT TGGTTTTGAA 
GATCATGATG AGACAGGAGG TGATGAAGGG GGTTCTGGAA GTTCTAATTA CAAAATTAAA 
TATTTTGGCT TTGACGATCT CAGCGAAAGT GAAGATGATG ATGATGACGA CTGTCAAGTG 
GAAAGAAAGA AAGACAAAAA AAGAACTAAA ACAGCTCCAT CACCTTCCCA GCAGCCTCCT 
CCTGAAAGCA GCGACAATTC CCAGGATAGT CAGTCTAGTA CTAATAATGC AGAAAACTTG 
GATTTTACAG AGGACTTGCC TGGTGTGCCT GAGAGTGTGA AGAAGCCCAT AAGTAAACAA 
GGAGATAAAT CCAAGGAAAA TACCAGAAAG ATTTTTAGTG GCCCCAAACG GTCACCTACA 
AAAGCTGTAT ATAATGCCAG GCATTGGAAC CATCCAGACT CGGAAGAATT GCCTGGACCA 
CCAATAGCAA AACCTCAGCG TGTCACATTA TATACTGTTG TTCAGCACGT GAAACACTTC 
AATGATGTGG TGGAATTTGG TGAAAATCAA GAGTTCACTG ATGACATTGA ATACTTGTTA 
AGTGGCTTAA AGAGTACTCA GCCTCTAAAC ACACGTTGCC TTAGTGTTAT CAGCTTAGCT 
ACTAAATGTG CCATGCCCAG TTTTCGGATG CATCTGAGGG CACATGGGAT GGTTGCAATG 
GTCTTTAAAA CTTTGGATGA TTCCCAGCAT CATCAGAATC TGTCCCTCTG TACAGCTGCT 
CTCATGTACA TATTGAGTAG AGACCGTTTG AACATGGATC TTGATAGGGC CAGCCTAGAT 
CTCATGATTC GGCTTTTGGA GTTGGAACAA GATGCCTCTT CAGCTAAGCT ACTGAATGAA 
AAAGACATGA ACAAGATCAA AGAAAAGATC CGAAGACTCT GTGAAACTGT GCACAACAAG 
CATCTTGATC TAGAAAACAT AACGACTGGT CATTTAGCTA TGGAGACATT GCTGTCCCTC 
ACTTCCAAAC GAGCAGGAGA TTGGTTTAAA GAAGAGCTCC GACTTCTGGG TGGTCTGGAT 
CATATTGTAG ATAAAGTAAA AGAGTGTGTG GATCATTTAA GTAGAGATGA TGAGGACGAA 
GAGAAACTAG TAGCCTCATT ATGGGGAGCA GAGAGATGTT TACGAGTTTT AGAGAGTGTA 
ACAGTGCATA ATCCAGAGAA TCAAAGCTAC TTGATAGCCT ATAAAGATTC ACAACTCATT 
ATTTCATCAG CTAAAGCATT ACAGCATTGT GAAGACCTGA TTCAGCAGTA CAACCGTGCT 
GAGAACAGCA TCTGTGTAGC AGACAGTAAC CCTCTGCCTT ACCAGAATGT AACTAACCAT 
GTGGGCAAAG CAGTGGAGGA CTGCATGAGG GCTATAATTG GAGTATTGCT CAATTTAACT 
AATGATAATG AGTGGGGCAG CACAAAGACA GGAGAACAAG AAGGACTCAT AGGCACAGCG 
ATGAACTGTG TTCTTCAGGT TCCAAAGTAC CTACCTCAGG AGCAGAGATT TGATATTCGA 
GTGCTGGGAT TGGGTCTACT CATAAACCTG GTGGAGTATA GTGCCCGGAA TCGACACTGC 
CTTGTCAACA TGCAAACATC CTGTTCCTTT GATTCCTCCT TCTCTAGTGG AGAAGGCGAT 
CATAGTTTAA GGCTAGCCGG ACAAGTTCAT GCTGTTCAAG CTTTAGTGCA GCTATTTCTC 
GAACGAGAGA GAGCAGCACA ATTGGCAGAA AGTAAAACAG ATGAATTGAT TAAAGATGCT 
CCTACCACTC AGCATGATAA GAGTGGAGAG TGGCAAGAAA CAAGTGGAGA AATACAGTGG 
GTATCAACTG AAAAGACTGA TGGTGCAGAG GAGAAGCAGA AGAAGGAGGA GGAGGATGAA 
GAACTTGACC TCAATAAAGC CCTTCAGCAT GCTGGCAAAC ACATGGAGGA TTGCATCGTA 
GCCTCCTACA CAGCCCTGCT TCTTGGGTGT CTCTGCCAGG AAAGTCCAAT CAATGTAACT 
ACAGTAAGGG AATATCTTCC AGAAGGAGAT TTCTCCATAA TGACAGAGAT GCTTAAAAAG 
TTCTTAAGCT TCATGAATCT TACGTGTGCT GTTGGAACAA CAGGCCAGAA GTCTATCTCT 
AGAGTGATTG AATATTTGGA ACATTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006518887.1
CDS	2005..5454
Translation	MTSRFGKTYSRKGGNGSSKFDEVFSNKRTTLSTKWGETTFMAKLGQKRPNFKPDIQEIPKKPKVEEEDTGDPFGFDSDDESLPVSSKNLAQGKGSSYSESSEAAQLEEVTSVFEANSKCSHVVGEDSFASDRCLLVEDTLIGKEKSISRIPEDNANKSSCTKLLTSDKVENFSEEHEKNSHHFHKNAEDSTKKPNAETAVASEYKADETKETNDTWNSQSGKRTESPSESCPVKGSVRTGLYEWDNDFEDIRSEDCILSLDNESLLEMKDEDLKNRIGGLENLNETFEEDIIQSVLRPSNCRTYCRANKARSSQGASNFDKLMDGTSQSLAKANSESSKDGLNQAKKGSASCGTSFRGTVGRTRDYTVLHPSCLSVCNVTIQDTMERSMDEFTASTPADLGEAGRLRKKADIATSKTTTRFRPSNTKSKKDVKLEFFGFEDHDETGGDEGGSGSSNYKIKYFGFDDLSESEDDDDDDCQVERKKDKKRTKTAPSPSQQPPPESSDNSQDSQSSTNNAENLDFTEDLPGVPESVKKPISKQGDKSKENTRKIFSGPKRSPTKAVYNARHWNHPDSEELPGPPIAKPQRVTLYTVVQHVKHFNDVVEFGENQEFTDDIEYLLSGLKSTQPLNTRCLSVISLATKCAMPSFRMHLRAHGMVAMVFKTLDDSQHHQNLSLCTAALMYILSRDRLNMDLDRASLDLMIRLLELEQDASSAKLLNEKDMNKIKEKIRRLCETVHNKHLDLENITTGHLAMETLLSLTSKRAGDWFKEELRLLGGLDHIVDKVKECVDHLSRDDEDEEKLVASLWGAERCLRVLESVTVHNPENQSYLIAYKDSQLIISSAKALQHCEDLIQQYNRAENSICVADSNPLPYQNVTNHVGKAVEDCMRAIIGVLLNLTNDNEWGSTKTGEQEGLIGTAMNCVLQVPKYLPQEQRFDIRVLGLGLLINLVEYSARNRHCLVNMQTSCSFDSSFSSGEGDHSLRLAGQVHAVQALVQLFLERERAAQLAESKTDELIKDAPTTQHDKSGEWQETSGEIQWVSTEKTDGAEEKQKKEEEDEELDLNKALQHAGKHMEDCIVASYTALLLGCLCQESPINVTTVREYLPEGDFSIMTEMLKKFLSFMNLTCAVGTTGQKSISRVIEYLEHC

Target ORF information:

RefSeq Version	XM_006518824.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus WAPL cohesin release factor (Wapl), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006518824.4

ORF Insert Sequence:

ATGACATCCA GATTTGGAAA AACTTACAGT AGGAAAGGAG GAAATGGCAG TTCAAAATTT 
GATGAAGTTT TTTCCAACAA ACGGACTACT CTTAGTACAA AATGGGGTGA GACCACATTT 
ATGGCTAAAT TAGGGCAGAA GAGGCCCAAT TTCAAACCAG ATATTCAAGA AATTCCGAAG 
AAACCTAAAG TAGAAGAAGA AGATACTGGA GATCCCTTTG GTTTTGATAG TGATGATGAG 
TCTCTACCTG TTTCTTCAAA AAATTTAGCC CAGGGTAAGG GTTCATCTTA CTCAGAATCT 
AGTGAGGCTG CTCAGCTGGA AGAAGTCACT TCTGTATTTG AAGCTAATAG CAAATGTAGT 
CATGTGGTGG GTGAAGACAG TTTTGCTTCC GACAGATGCT TACTTGTGGA GGATACTTTA 
ATTGGGAAAG AGAAGAGCAT AAGTAGAATT CCAGAAGACA ACGCAAACAA AAGTAGTTGC 
ACTAAGTTGC TAACTTCAGA TAAAGTGGAG AATTTTAGTG AAGAACATGA AAAAAATAGT 
CACCACTTTC ACAAAAATGC TGAAGATAGT ACTAAGAAAC CCAATGCAGA AACCGCAGTG 
GCTTCTGAAT ATAAAGCTGA TGAAACTAAA GAAACAAATG ATACTTGGAA CTCCCAGTCT 
GGAAAAAGAA CAGAGTCTCC ATCTGAAAGT TGTCCAGTCA AAGGATCTGT AAGAACTGGT 
TTATATGAAT GGGATAATGA TTTTGAAGAT ATCAGGTCAG AAGACTGTAT TTTAAGTTTG 
GATAATGAGT CTCTTTTGGA GATGAAAGAC GAGGATTTAA AAAATCGGAT TGGAGGATTG 
GAAAATCTAA ATGAAACCTT TGAAGAAGAT ATCATACAAA GTGTTCTTAG GCCAAGCAAC 
TGTAGGACGT ACTGTAGGGC CAATAAAGCG AGATCCTCAC AGGGAGCATC AAATTTTGAT 
AAGCTAATGG ATGGCACCAG TCAGTCCTTA GCCAAAGCAA ACAGTGAATC AAGTAAAGAT 
GGCCTGAATC AGGCAAAGAA AGGTAGTGCA AGTTGTGGGA CCAGTTTTCG AGGAACAGTT 
GGACGGACTA GAGATTACAC TGTTTTACAT CCATCTTGCT TGTCAGTGTG TAATGTTACC 
ATCCAGGATA CTATGGAACG GAGTATGGAT GAGTTCACCG CATCCACTCC TGCAGATTTA 
GGAGAGGCTG GCCGGCTCAG AAAAAAGGCA GATATTGCAA CCTCCAAGAC CACTACTAGA 
TTTCGACCTA GTAATACTAA ATCCAAAAAG GATGTTAAAC TTGAATTTTT TGGTTTTGAA 
GATCATGATG AGACAGGAGG TGATGAAGGG GGTTCTGGAA GTTCTAATTA CAAAATTAAA 
TATTTTGGCT TTGACGATCT CAGCGAAAGT GAAGATGATG ATGATGACGA CTGTCAAGTG 
GAAAGAAAGA AAGACAAAAA AAGAACTAAA ACAGCTCCAT CACCTTCCCA GCAGCCTCCT 
CCTGAAAGCA GCGACAATTC CCAGGATAGT CAGTCTAGTA CTAATAATGC AGAAAACTTG 
GATTTTACAG AGGACTTGCC TGGTGTGCCT GAGAGTGTGA AGAAGCCCAT AAGTAAACAA 
GGAGATAAAT CCAAGGAAAA TACCAGAAAG ATTTTTAGTG GCCCCAAACG GTCACCTACA 
AAAGCTGTAT ATAATGCCAG GCATTGGAAC CATCCAGACT CGGAAGAATT GCCTGGACCA 
CCAATAGCAA AACCTCAGCG TGTCACATTA TATACTGTTG TTCAGCACGT GAAACACTTC 
AATGATGTGG TGGAATTTGG TGAAAATCAA GAGTTCACTG ATGACATTGA ATACTTGTTA 
AGTGGCTTAA AGAGTACTCA GCCTCTAAAC ACACGTTGCC TTAGTGTTAT CAGCTTAGCT 
ACTAAATGTG CCATGCCCAG TTTTCGGATG CATCTGAGGG CACATGGGAT GGTTGCAATG 
GTCTTTAAAA CTTTGGATGA TTCCCAGCAT CATCAGAATC TGTCCCTCTG TACAGCTGCT 
CTCATGTACA TATTGAGTAG AGACCGTTTG AACATGGATC TTGATAGGGC CAGCCTAGAT 
CTCATGATTC GGCTTTTGGA GTTGGAACAA GATGCCTCTT CAGCTAAGCT ACTGAATGAA 
AAAGACATGA ACAAGATCAA AGAAAAGATC CGAAGACTCT GTGAAACTGT GCACAACAAG 
CATCTTGATC TAGAAAACAT AACGACTGGT CATTTAGCTA TGGAGACATT GCTGTCCCTC 
ACTTCCAAAC GAGCAGGAGA TTGGTTTAAA GAAGAGCTCC GACTTCTGGG TGGTCTGGAT 
CATATTGTAG ATAAAGTAAA AGAGTGTGTG GATCATTTAA GTAGAGATGA TGAGGACGAA 
GAGAAACTAG TAGCCTCATT ATGGGGAGCA GAGAGATGTT TACGAGTTTT AGAGAGTGTA 
ACAGTGCATA ATCCAGAGAA TCAAAGCTAC TTGATAGCCT ATAAAGATTC ACAACTCATT 
ATTTCATCAG CTAAAGCATT ACAGCATTGT GAAGACCTGA TTCAGCAGTA CAACCGTGCT 
GAGAACAGCA TCTGTGTAGC AGACAGTAAC CCTCTGCCTT ACCAGAATGT AACTAACCAT 
GTGGGCAAAG CAGTGGAGGA CTGCATGAGG GCTATAATTG GAGTATTGCT CAATTTAACT 
AATGATAATG AGTGGGGCAG CACAAAGACA GGAGAACAAG AAGGACTCAT AGGCACAGCG 
ATGAACTGTG TTCTTCAGGT TCCAAAGTAC CTACCTCAGG AGCAGAGATT TGATATTCGA 
GTGCTGGGAT TGGGTCTACT CATAAACCTG GTGGAGTATA GTGCCCGGAA TCGACACTGC 
CTTGTCAACA TGCAAACATC CTGTTCCTTT GATTCCTCCT TCTCTAGTGG AGAAGGCGAT 
CATAGTTTAA GGCTAGCCGG ACAAGTTCAT GCTGTTCAAG CTTTAGTGCA GCTATTTCTC 
GAACGAGAGA GAGCAGCACA ATTGGCAGAA AGTAAAACAG ATGAATTGAT TAAAGATGCT 
CCTACCACTC AGCATGATAA GAGTGGAGAG TGGCAAGAAA CAAGTGGAGA AATACAGTGG 
GTATCAACTG AAAAGACTGA TGGTGCAGAG GAGAAGCAGA AGAAGGAGGA GGAGGATGAA 
GAACTTGACC TCAATAAAGC CCTTCAGCAT GCTGGCAAAC ACATGGAGGA TTGCATCGTA 
GCCTCCTACA CAGCCCTGCT TCTTGGGTGT CTCTGCCAGG AAAGTCCAAT CAATGTAACT 
ACAGTAAGGG AATATCTTCC AGAAGGAGAT TTCTCCATAA TGACAGAGAT GCTTAAAAAG 
TTCTTAAGCT TCATGAATCT TACGTGTGCT GTTGGAACAA CAGGCCAGAA GTCTATCTCT 
AGAGTGATTG AATATTTGGA ACATTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Wapl ( NM_001004436.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001004436.2 Mus musculus WAPL cohesin release factor (Wapl), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu10653
Clone ID Related Accession (Same CDS sequence)	NM_001004436.4
Accession Version	NM_001004436.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Mus musculus(house mouse)
Product	wings apart-like protein homolog isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CJ134128.1, BY732412.1, BC139434.1, CJ061782.1, DV659439.1, CJ053978.1, DV646537.1, BG068179.2 and BP757948.1. On Aug 6, 2014 this sequence version replaced NM_001004436.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACATCCA GATTTGGAAA AACTTACAGT AGGAAAGGAG GAAATGGCAG TTCAAAATTT 
GATGAAGTTT TTTCCAACAA ACGGACTACT CTTAGTACAA AATGGGGTGA GACCACATTT 
ATGGCTAAAT TAGGGCAGAA GAGGCCCAAT TTCAAACCAG ATATTCAAGA AATTCCGAAG 
AAACCTAAAG TAGAAGAAGA AGATACTGGA GATCCCTTTG GTTTTGATAG TGATGATGAG 
TCTCTACCTG TTTCTTCAAA AAATTTAGCC CAGGGTAAGG GTTCATCTTA CTCAGAATCT 
AGTGAGGCTG CTCAGCTGGA AGAAGTCACT TCTGTATTTG AAGCTAATAG CAAATGTAGT 
CATGTGGTGG GTGAAGACAG TTTTGCTTCC GACAGATGCT TACTTGTGGA GGATACTTTA 
ATTGGGAAAG AGAAGAGCAT AAGTAGAATT CCAGAAGACA ACGCAAACAA AAGTAGTTGC 
ACTAAGTTGC TAACTTCAGA TAAAGTGGAG AATTTTAGTG AAGAACATGA AAAAAATAGT 
CACCACTTTC ACAAAAATGC TGAAGATAGT ACTAAGAAAC CCAATGCAGA AACCGCAGTG 
GCTTCTGAAT ATAAAGCTGA TGAAACTAAA GAAACAAATG ATACTTGGAA CTCCCAGTCT 
GGAAAAAGAA CAGAGTCTCC ATCTGAAAGT TGTCCAGTCA AAGGATCTGT AAGAACTGGT 
TTATATGAAT GGGATAATGA TTTTGAAGAT ATCAGGTCAG AAGACTGTAT TTTAAGTTTG 
GATAATGAGT CTCTTTTGGA GATGAAAGAC GAGGATTTAA AAAATCGGAT TGGAGGATTG 
GAAAATCTAA ATGAAACCTT TGAAGAAGAT ATCATACAAA GTGTTCTTAG GCCAAGCAAC 
TGTAGGACGT ACTGTAGGGC CAATAAAGCG AGATCCTCAC AGGGAGCATC AAATTTTGAT 
AAGCTAATGG ATGGCACCAG TCAGTCCTTA GCCAAAGCAA ACAGTGAATC AAGTAAAGAT 
GGCCTGAATC AGGCAAAGAA AGGTAGTGCA AGTTGTGGGA CCAGTTTTCG AGGAACAGTT 
GGACGGACTA GAGATTACAC TGTTTTACAT CCATCTTGCT TGTCAGTGTG TAATGTTACC 
ATCCAGGATA CTATGGAACG GAGTATGGAT GAGTTCACCG CATCCACTCC TGCAGATTTA 
GGAGAGGCTG GCCGGCTCAG AAAAAAGGCA GATATTGCAA CCTCCAAGAC CACTACTAGA 
TTTCGACCTA GTAATACTAA ATCCAAAAAG GATGTTAAAC TTGAATTTTT TGGTTTTGAA 
GATCATGATG AGACAGGAGG TGATGAAGGG GGTTCTGGAA GTTCTAATTA CAAAATTAAA 
TATTTTGGCT TTGACGATCT CAGCGAAAGT GAAGATGATG ATGATGACGA CTGTCAAGTG 
GAAAGAAAGA AAGACAAAAA AAGAACTAAA ACAGCTCCAT CACCTTCCCA GCAGCCTCCT 
CCTGAAAGCA GCGACAATTC CCAGGATAGT CAGTCTAGTA CTAATAATGC AGAAAACTTG 
GATTTTACAG AGGACTTGCC TGGTGTGCCT GAGAGTGTGA AGAAGCCCAT AAGTAAACAA 
GGAGATAAAT CCAAGGAAAA TACCAGAAAG ATTTTTAGTG GCCCCAAACG GTCACCTACA 
AAAGCTGTAT ATAATGCCAG GCATTGGAAC CATCCAGACT CGGAAGAATT GCCTGGACCA 
CCAATAGCAA AACCTCAGCG TGTCACAGTG AGGCTGTCTT CAAAGGAACC AAATCAAAAA 
GATGATGGAG TTTTTAAGGC TCCTGCACCA CCACTCAAAG TGATAAAAAC TGTGACAATA 
CCTACTCAGC CCTACCAAGA AATAGTTACT GCACTGAAAT GCAGAAAAGA AGACAAAGAA 
TTATATACTG TTGTTCAGCA CGTGAAACAC TTCAATGATG TGGTGGAATT TGGTGAAAAT 
CAAGAGTTCA CTGATGACAT TGAATACTTG TTAAGTGGCT TAAAGAGTAC TCAGCCTCTA 
AACACACGTT GCCTTAGTGT TATCAGCTTA GCTACTAAAT GTGCCATGCC CAGTTTTCGG 
ATGCATCTGA GGGCACATGG GATGGTTGCA ATGGTCTTTA AAACTTTGGA TGATTCCCAG 
CATCATCAGA ATCTGTCCCT CTGTACAGCT GCTCTCATGT ACATATTGAG TAGAGACCGT 
TTGAACATGG ATCTTGATAG GGCCAGCCTA GATCTCATGA TTCGGCTTTT GGAGTTGGAA 
CAAGATGCCT CTTCAGCTAA GCTACTGAAT GAAAAAGACA TGAACAAGAT CAAAGAAAAG 
ATCCGAAGAC TCTGTGAAAC TGTGCACAAC AAGCATCTTG ATCTAGAAAA CATAACGACT 
GGTCATTTAG CTATGGAGAC ATTGCTGTCC CTCACTTCCA AACGAGCAGG AGATTGGTTT 
AAAGAAGAGC TCCGACTTCT GGGTGGTCTG GATCATATTG TAGATAAAGT AAAAGAGTGT 
GTGGATCATT TAAGTAGAGA TGATGAGGAC GAAGAGAAAC TAGTAGCCTC ATTATGGGGA 
GCAGAGAGAT GTTTACGAGT TTTAGAGAGT GTAACAGTGC ATAATCCAGA GAATCAAAGC 
TACTTGATAG CCTATAAAGA TTCACAACTC ATTATTTCAT CAGCTAAAGC ATTACAGCAT 
TGTGAAGACC TGATTCAGCA GTACAACCGT GCTGAGAACA GCATCTGTGT AGCAGACAGT 
AACCCTCTGC CTTACCAGAA TGTAACTAAC CATGTGGGCA AAGCAGTGGA GGACTGCATG 
AGGGCTATAA TTGGAGTATT GCTCAATTTA ACTAATGATA ATGAGTGGGG CAGCACAAAG 
ACAGGAGAAC AAGAAGGACT CATAGGCACA GCGATGAACT GTGTTCTTCA GGTTCCAAAG 
TACCTACCTC AGGAGCAGAG ATTTGATATT CGAGTGCTGG GATTGGGTCT ACTCATAAAC 
CTGGTGGAGT ATAGTGCCCG GAATCGACAC TGCCTTGTCA ACATGCAAAC ATCCTGTTCC 
TTTGATTCCT CCTTCTCTAG TGGAGAAGGC GATCATAGTT TAAGGCTAGC CGGACAAGTT 
CATGCTGTTC AAGCTTTAGT GCAGCTATTT CTCGAACGAG AGAGAGCAGC ACAATTGGCA 
GAAAGTAAAA CAGATGAATT GATTAAAGAT GCTCCTACCA CTCAGCATGA TAAGAGTGGA 
GAGTGGCAAG AAACAAGTGG AGAAATACAG TGGGTATCAA CTGAAAAGAC TGATGGTGCA 
GAGGAGAAGC AGAAGAAGGA GGAGGAGGAT GAAGAACTTG ACCTCAATAA AGCCCTTCAG 
CATGCTGGCA AACACATGGA GGATTGCATC GTAGCCTCCT ACACAGCCCT GCTTCTTGGG 
TGTCTCTGCC AGGAAAGTCC AATCAATGTA ACTACAGTAA GGGAATATCT TCCAGAAGGA 
GATTTCTCCA TAATGACAGA GATGCTTAAA AAGTTCTTAA GCTTCATGAA TCTTACGTGT 
GCTGTTGGAA CAACAGGCCA GAAGTCTATC TCTAGAGTGA TTGAATATTT GGAACATTGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001004436.2
CDS	456..4058
Translation	MTSRFGKTYSRKGGNGSSKFDEVFSNKRTTLSTKWGETTFMAKLGQKRPNFKPDIQEIPKKPKVEEEDTGDPFGFDSDDESLPVSSKNLAQGKGSSYSESSEAAQLEEVTSVFEANSKCSHVVGEDSFASDRCLLVEDTLIGKEKSISRIPEDNANKSSCTKLLTSDKVENFSEEHEKNSHHFHKNAEDSTKKPNAETAVASEYKADETKETNDTWNSQSGKRTESPSESCPVKGSVRTGLYEWDNDFEDIRSEDCILSLDNESLLEMKDEDLKNRIGGLENLNETFEEDIIQSVLRPSNCRTYCRANKARSSQGASNFDKLMDGTSQSLAKANSESSKDGLNQAKKGSASCGTSFRGTVGRTRDYTVLHPSCLSVCNVTIQDTMERSMDEFTASTPADLGEAGRLRKKADIATSKTTTRFRPSNTKSKKDVKLEFFGFEDHDETGGDEGGSGSSNYKIKYFGFDDLSESEDDDDDDCQVERKKDKKRTKTAPSPSQQPPPESSDNSQDSQSSTNNAENLDFTEDLPGVPESVKKPISKQGDKSKENTRKIFSGPKRSPTKAVYNARHWNHPDSEELPGPPIAKPQRVTVRLSSKEPNQKDDGVFKAPAPPLKVIKTVTIPTQPYQEIVTALKCRKEDKELYTVVQHVKHFNDVVEFGENQEFTDDIEYLLSGLKSTQPLNTRCLSVISLATKCAMPSFRMHLRAHGMVAMVFKTLDDSQHHQNLSLCTAALMYILSRDRLNMDLDRASLDLMIRLLELEQDASSAKLLNEKDMNKIKEKIRRLCETVHNKHLDLENITTGHLAMETLLSLTSKRAGDWFKEELRLLGGLDHIVDKVKECVDHLSRDDEDEEKLVASLWGAERCLRVLESVTVHNPENQSYLIAYKDSQLIISSAKALQHCEDLIQQYNRAENSICVADSNPLPYQNVTNHVGKAVEDCMRAIIGVLLNLTNDNEWGSTKTGEQEGLIGTAMNCVLQVPKYLPQEQRFDIRVLGLGLLINLVEYSARNRHCLVNMQTSCSFDSSFSSGEGDHSLRLAGQVHAVQALVQLFLERERAAQLAESKTDELIKDAPTTQHDKSGEWQETSGEIQWVSTEKTDGAEEKQKKEEEDEELDLNKALQHAGKHMEDCIVASYTALLLGCLCQESPINVTTVREYLPEGDFSIMTEMLKKFLSFMNLTCAVGTTGQKSISRVIEYLEHC

Target ORF information:

RefSeq Version	NM_001004436.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus WAPL cohesin release factor (Wapl), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001004436.4

ORF Insert Sequence:

ATGACATCCA GATTTGGAAA AACTTACAGT AGGAAAGGAG GAAATGGCAG TTCAAAATTT 
GATGAAGTTT TTTCCAACAA ACGGACTACT CTTAGTACAA AATGGGGTGA GACCACATTT 
ATGGCTAAAT TAGGGCAGAA GAGGCCCAAT TTCAAACCAG ATATTCAAGA AATTCCGAAG 
AAACCTAAAG TAGAAGAAGA AGATACTGGA GATCCCTTTG GTTTTGATAG TGATGATGAG 
TCTCTACCTG TTTCTTCAAA AAATTTAGCC CAGGGTAAGG GTTCATCTTA CTCAGAATCT 
AGTGAGGCTG CTCAGCTGGA AGAAGTCACT TCTGTATTTG AAGCTAATAG CAAATGTAGT 
CATGTGGTGG GTGAAGACAG TTTTGCTTCC GACAGATGCT TACTTGTGGA GGATACTTTA 
ATTGGGAAAG AGAAGAGCAT AAGTAGAATT CCAGAAGACA ACGCAAACAA AAGTAGTTGC 
ACTAAGTTGC TAACTTCAGA TAAAGTGGAG AATTTTAGTG AAGAACATGA AAAAAATAGT 
CACCACTTTC ACAAAAATGC TGAAGATAGT ACTAAGAAAC CCAATGCAGA AACCGCAGTG 
GCTTCTGAAT ATAAAGCTGA TGAAACTAAA GAAACAAATG ATACTTGGAA CTCCCAGTCT 
GGAAAAAGAA CAGAGTCTCC ATCTGAAAGT TGTCCAGTCA AAGGATCTGT AAGAACTGGT 
TTATATGAAT GGGATAATGA TTTTGAAGAT ATCAGGTCAG AAGACTGTAT TTTAAGTTTG 
GATAATGAGT CTCTTTTGGA GATGAAAGAC GAGGATTTAA AAAATCGGAT TGGAGGATTG 
GAAAATCTAA ATGAAACCTT TGAAGAAGAT ATCATACAAA GTGTTCTTAG GCCAAGCAAC 
TGTAGGACGT ACTGTAGGGC CAATAAAGCG AGATCCTCAC AGGGAGCATC AAATTTTGAT 
AAGCTAATGG ATGGCACCAG TCAGTCCTTA GCCAAAGCAA ACAGTGAATC AAGTAAAGAT 
GGCCTGAATC AGGCAAAGAA AGGTAGTGCA AGTTGTGGGA CCAGTTTTCG AGGAACAGTT 
GGACGGACTA GAGATTACAC TGTTTTACAT CCATCTTGCT TGTCAGTGTG TAATGTTACC 
ATCCAGGATA CTATGGAACG GAGTATGGAT GAGTTCACCG CATCCACTCC TGCAGATTTA 
GGAGAGGCTG GCCGGCTCAG AAAAAAGGCA GATATTGCAA CCTCCAAGAC CACTACTAGA 
TTTCGACCTA GTAATACTAA ATCCAAAAAG GATGTTAAAC TTGAATTTTT TGGTTTTGAA 
GATCATGATG AGACAGGAGG TGATGAAGGG GGTTCTGGAA GTTCTAATTA CAAAATTAAA 
TATTTTGGCT TTGACGATCT CAGCGAAAGT GAAGATGATG ATGATGACGA CTGTCAAGTG 
GAAAGAAAGA AAGACAAAAA AAGAACTAAA ACAGCTCCAT CACCTTCCCA GCAGCCTCCT 
CCTGAAAGCA GCGACAATTC CCAGGATAGT CAGTCTAGTA CTAATAATGC AGAAAACTTG 
GATTTTACAG AGGACTTGCC TGGTGTGCCT GAGAGTGTGA AGAAGCCCAT AAGTAAACAA 
GGAGATAAAT CCAAGGAAAA TACCAGAAAG ATTTTTAGTG GCCCCAAACG GTCACCTACA 
AAAGCTGTAT ATAATGCCAG GCATTGGAAC CATCCAGACT CGGAAGAATT GCCTGGACCA 
CCAATAGCAA AACCTCAGCG TGTCACAGTG AGGCTGTCTT CAAAGGAACC AAATCAAAAA 
GATGATGGAG TTTTTAAGGC TCCTGCACCA CCACTCAAAG TGATAAAAAC TGTGACAATA 
CCTACTCAGC CCTACCAAGA AATAGTTACT GCACTGAAAT GCAGAAAAGA AGACAAAGAA 
TTATATACTG TTGTTCAGCA CGTGAAACAC TTCAATGATG TGGTGGAATT TGGTGAAAAT 
CAAGAGTTCA CTGATGACAT TGAATACTTG TTAAGTGGCT TAAAGAGTAC TCAGCCTCTA 
AACACACGTT GCCTTAGTGT TATCAGCTTA GCTACTAAAT GTGCCATGCC CAGTTTTCGG 
ATGCATCTGA GGGCACATGG GATGGTTGCA ATGGTCTTTA AAACTTTGGA TGATTCCCAG 
CATCATCAGA ATCTGTCCCT CTGTACAGCT GCTCTCATGT ACATATTGAG TAGAGACCGT 
TTGAACATGG ATCTTGATAG GGCCAGCCTA GATCTCATGA TTCGGCTTTT GGAGTTGGAA 
CAAGATGCCT CTTCAGCTAA GCTACTGAAT GAAAAAGACA TGAACAAGAT CAAAGAAAAG 
ATCCGAAGAC TCTGTGAAAC TGTGCACAAC AAGCATCTTG ATCTAGAAAA CATAACGACT 
GGTCATTTAG CTATGGAGAC ATTGCTGTCC CTCACTTCCA AACGAGCAGG AGATTGGTTT 
AAAGAAGAGC TCCGACTTCT GGGTGGTCTG GATCATATTG TAGATAAAGT AAAAGAGTGT 
GTGGATCATT TAAGTAGAGA TGATGAGGAC GAAGAGAAAC TAGTAGCCTC ATTATGGGGA 
GCAGAGAGAT GTTTACGAGT TTTAGAGAGT GTAACAGTGC ATAATCCAGA GAATCAAAGC 
TACTTGATAG CCTATAAAGA TTCACAACTC ATTATTTCAT CAGCTAAAGC ATTACAGCAT 
TGTGAAGACC TGATTCAGCA GTACAACCGT GCTGAGAACA GCATCTGTGT AGCAGACAGT 
AACCCTCTGC CTTACCAGAA TGTAACTAAC CATGTGGGCA AAGCAGTGGA GGACTGCATG 
AGGGCTATAA TTGGAGTATT GCTCAATTTA ACTAATGATA ATGAGTGGGG CAGCACAAAG 
ACAGGAGAAC AAGAAGGACT CATAGGCACA GCGATGAACT GTGTTCTTCA GGTTCCAAAG 
TACCTACCTC AGGAGCAGAG ATTTGATATT CGAGTGCTGG GATTGGGTCT ACTCATAAAC 
CTGGTGGAGT ATAGTGCCCG GAATCGACAC TGCCTTGTCA ACATGCAAAC ATCCTGTTCC 
TTTGATTCCT CCTTCTCTAG TGGAGAAGGC GATCATAGTT TAAGGCTAGC CGGACAAGTT 
CATGCTGTTC AAGCTTTAGT GCAGCTATTT CTCGAACGAG AGAGAGCAGC ACAATTGGCA 
GAAAGTAAAA CAGATGAATT GATTAAAGAT GCTCCTACCA CTCAGCATGA TAAGAGTGGA 
GAGTGGCAAG AAACAAGTGG AGAAATACAG TGGGTATCAA CTGAAAAGAC TGATGGTGCA 
GAGGAGAAGC AGAAGAAGGA GGAGGAGGAT GAAGAACTTG ACCTCAATAA AGCCCTTCAG 
CATGCTGGCA AACACATGGA GGATTGCATC GTAGCCTCCT ACACAGCCCT GCTTCTTGGG 
TGTCTCTGCC AGGAAAGTCC AATCAATGTA ACTACAGTAA GGGAATATCT TCCAGAAGGA 
GATTTCTCCA TAATGACAGA GATGCTTAAA AAGTTCTTAA GCTTCATGAA TCTTACGTGT 
GCTGTTGGAA CAACAGGCCA GAAGTCTATC TCTAGAGTGA TTGAATATTT GGAACATTGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Wapl ( NM_001301330.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001288259.1 Mus musculus WAPL cohesin release factor (Wapl), transcript variant 2, mRNA.

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CloneID	OMu73083
Clone ID Related Accession (Same CDS sequence)	NM_001301330.1
Accession Version	NM_001301330.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Mus musculus(house mouse)
Product	wings apart-like protein homolog isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CJ134128.1, BY732412.1, BC139434.1, CF161743.1, CJ061782.1, DV659439.1, CJ053978.1, DV646537.1, BG068179.2 and BP757948.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACATCCA GATTTGGAAA AACTTACAGT AGGAAAGGAG GAAATGGCAG TTCAAAATTT 
GATGAAGTTT TTTCCAACAA ACGGACTACT CTTAGTACAA AATGGGGTGA GACCACATTT 
ATGGCTAAAT TAGGGCAGAA GAGGCCCAAT TTCAAACCAG ATATTCAAGA AATTCCGAAG 
AAACCTAAAG TAGAAGAAGA AGATACTGGA GATCCCTTTG GTTTTGATAG TGATGATGAG 
TCTCTACCTG TTTCTTCAAA AAATTTAGCC CAGGGTAAGG GTTCATCTTA CTCAGAATCT 
AGTGAGGCTG CTCAGCTGGA AGAAGTCACT TCTGTATTTG AAGCTAATAG CAAATGTAGT 
CATGTGGTGG GTGAAGACAG TTTTGCTTCC GACAGATGCT TACTTGTGGA GGATACTTTA 
ATTGGGAAAG AGAAGAGCAT AAGTAGAATT CCAGAAGACA ACGCAAACAA AAGTAGTTGC 
ACTAAGTTGC TAACTTCAGA TAAAGTGGAG AATTTTAGTG AAGAACATGA AAAAAATAGT 
CACCACTTTC ACAAAAATGC TGAAGATAGT ACTAAGAAAC CCAATGCAGA AACCGCAGTG 
GCTTCTGAAT ATAAAGCTGA TGAAACTAAA GAAACAAATG ATACTTGGAA CTCCCAGTCT 
GGAAAAAGAA CAGAGTCTCC ATCTGAAAGT TGTCCAGTCA AAGGATCTGT AAGAACTGGT 
TTATATGAAT GGGATAATGA TTTTGAAGAT ATCAGGTCAG AAGACTGTAT TTTAAGTTTG 
GATAATGAGT CTCTTTTGGA GATGAAAGAC GAGGATTTAA AAAATCGGAT TGGAGGATTG 
GAAAATCTAA ATGAAACCTT TGAAGAAGAT ATCATACAAA GTGTTCTTAG GCCAAGCAAC 
TGTAGGACGT ACTGTAGGGC CAATAAAGCG AGATCCTCAC AGGGAGCATC AAATTTTGAT 
AAGCTAATGG ATGGCACCAG TCAGTCCTTA GCCAAAGCAA ACAGTGAATC AAGTAAAGAT 
GGCCTGAATC AGGCAAAGAA AGGTAGTGCA AGTTGTGGGA CCAGTTTTCG AGGAACAGTT 
GGACGGACTA GAGATTACAC TGTTTTACAT CCATCTTGCT TGTCAGTGTG TAATGTTACC 
ATCCAGGATA CTATGGAACG GAGTATGGAT GAGTTCACCG CATCCACTCC TGCAGATTTA 
GGAGAGGCTG GCCGGCTCAG AAAAAAGGCA GATATTGCAA CCTCCAAGAC CACTACTAGA 
TTTCGACCTA GTAATACTAA ATCCAAAAAG GATGTTAAAC TTGAATTTTT TGGTTTTGAA 
GATCATGATG AGACAGGAGG TGATGAAGGG GGTTCTGGAA GTTCTAATTA CAAAATTAAA 
TATTTTGGCT TTGACGATCT CAGCGAAAGT GAAGATGATG ATGATGACGA CTGTCAAGTG 
GAAAGAAAGA AAGACAAAAA AAGAACTAAA ACAGCTCCAT CACCTTCCCA GCAGCCTCCT 
CCTGAAAGCA GCGACAATTC CCAGGATAGT CAGTCTAGTA CTAATAATGC AGAGGACTTG 
CCTGGTGTGC CTGAGAGTGT GAAGAAGCCC ATAAGTAAAC AAGGAGATAA ATCCAAGGAA 
AATACCAGAA AGATTTTTAG TGGCCCCAAA CGGTCACCTA CAAAAGCTGT ATATAATGCC 
AGGCATTGGA ACCATCCAGA CTCGGAAGAA TTGCCTGGAC CACCAATAGC AAAACCTCAG 
CGTGTCACAG TGAGGCTGTC TTCAAAGGAA CCAAATCAAA AAGATGATGG AGTTTTTAAG 
GCTCCTGCAC CACCACTCAA AGTGATAAAA ACTGTGACAA TACCTACTCA GCCCTACCAA 
GAAATAGTTA CTGCACTGAA ATGCAGAAAA GAAGACAAAG AATTATATAC TGTTGTTCAG 
CACGTGAAAC ACTTCAATGA TGTGGTGGAA TTTGGTGAAA ATCAAGAGTT CACTGATGAC 
ATTGAATACT TGTTAAGTGG CTTAAAGAGT ACTCAGCCTC TAAACACACG TTGCCTTAGT 
GTTATCAGCT TAGCTACTAA ATGTGCCATG CCCAGTTTTC GGATGCATCT GAGGGCACAT 
GGGATGGTTG CAATGGTCTT TAAAACTTTG GATGATTCCC AGCATCATCA GAATCTGTCC 
CTCTGTACAG CTGCTCTCAT GTACATATTG AGTAGAGACC GTTTGAACAT GGATCTTGAT 
AGGGCCAGCC TAGATCTCAT GATTCGGCTT TTGGAGTTGG AACAAGATGC CTCTTCAGCT 
AAGCTACTGA ATGAAAAAGA CATGAACAAG ATCAAAGAAA AGATCCGAAG ACTCTGTGAA 
ACTGTGCACA ACAAGCATCT TGATCTAGAA AACATAACGA CTGGTCATTT AGCTATGGAG 
ACATTGCTGT CCCTCACTTC CAAACGAGCA GGAGATTGGT TTAAAGAAGA GCTCCGACTT 
CTGGGTGGTC TGGATCATAT TGTAGATAAA GTAAAAGAGT GTGTGGATCA TTTAAGTAGA 
GATGATGAGG ACGAAGAGAA ACTAGTAGCC TCATTATGGG GAGCAGAGAG ATGTTTACGA 
GTTTTAGAGA GTGTAACAGT GCATAATCCA GAGAATCAAA GCTACTTGAT AGCCTATAAA 
GATTCACAAC TCATTATTTC ATCAGCTAAA GCATTACAGC ATTGTGAAGA CCTGATTCAG 
CAGTACAACC GTGCTGAGAA CAGCATCTGT GTAGCAGACA GTAACCCTCT GCCTTACCAG 
AATGTAACTA ACCATGTGGG CAAAGCAGTG GAGGACTGCA TGAGGGCTAT AATTGGAGTA 
TTGCTCAATT TAACTAATGA TAATGAGTGG GGCAGCACAA AGACAGGAGA ACAAGAAGGA 
CTCATAGGCA CAGCGATGAA CTGTGTTCTT CAGGTTCCAA AGTACCTACC TCAGGAGCAG 
AGATTTGATA TTCGAGTGCT GGGATTGGGT CTACTCATAA ACCTGGTGGA GTATAGTGCC 
CGGAATCGAC ACTGCCTTGT CAACATGCAA ACATCCTGTT CCTTTGATTC CTCCTTCTCT 
AGTGGAGAAG GCGATCATAG TTTAAGGCTA GCCGGACAAG TTCATGCTGT TCAAGCTTTA 
GTGCAGCTAT TTCTCGAACG AGAGAGAGCA GCACAATTGG CAGAAAGTAA AACAGATGAA 
TTGATTAAAG ATGCTCCTAC CACTCAGCAT GATAAGAGTG GAGAGTGGCA AGAAACAAGT 
GGAGAAATAC AGTGGGTATC AACTGAAAAG ACTGATGGTG CAGAGGAGAA GCAGAAGAAG 
GAGGAGGAGG ATGAAGAACT TGACCTCAAT AAAGCCCTTC AGCATGCTGG CAAACACATG 
GAGGATTGCA TCGTAGCCTC CTACACAGCC CTGCTTCTTG GGTGTCTCTG CCAGGAAAGT 
CCAATCAATG TAACTACAGT AAGGGAATAT CTTCCAGAAG GAGATTTCTC CATAATGACA 
GAGATGCTTA AAAAGTTCTT AAGCTTCATG AATCTTACGT GTGCTGTTGG AACAACAGGC 
CAGAAGTCTA TCTCTAGAGT GATTGAATAT TTGGAACATT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001288259.1
CDS	456..4040
Translation	MTSRFGKTYSRKGGNGSSKFDEVFSNKRTTLSTKWGETTFMAKLGQKRPNFKPDIQEIPKKPKVEEEDTGDPFGFDSDDESLPVSSKNLAQGKGSSYSESSEAAQLEEVTSVFEANSKCSHVVGEDSFASDRCLLVEDTLIGKEKSISRIPEDNANKSSCTKLLTSDKVENFSEEHEKNSHHFHKNAEDSTKKPNAETAVASEYKADETKETNDTWNSQSGKRTESPSESCPVKGSVRTGLYEWDNDFEDIRSEDCILSLDNESLLEMKDEDLKNRIGGLENLNETFEEDIIQSVLRPSNCRTYCRANKARSSQGASNFDKLMDGTSQSLAKANSESSKDGLNQAKKGSASCGTSFRGTVGRTRDYTVLHPSCLSVCNVTIQDTMERSMDEFTASTPADLGEAGRLRKKADIATSKTTTRFRPSNTKSKKDVKLEFFGFEDHDETGGDEGGSGSSNYKIKYFGFDDLSESEDDDDDDCQVERKKDKKRTKTAPSPSQQPPPESSDNSQDSQSSTNNAEDLPGVPESVKKPISKQGDKSKENTRKIFSGPKRSPTKAVYNARHWNHPDSEELPGPPIAKPQRVTVRLSSKEPNQKDDGVFKAPAPPLKVIKTVTIPTQPYQEIVTALKCRKEDKELYTVVQHVKHFNDVVEFGENQEFTDDIEYLLSGLKSTQPLNTRCLSVISLATKCAMPSFRMHLRAHGMVAMVFKTLDDSQHHQNLSLCTAALMYILSRDRLNMDLDRASLDLMIRLLELEQDASSAKLLNEKDMNKIKEKIRRLCETVHNKHLDLENITTGHLAMETLLSLTSKRAGDWFKEELRLLGGLDHIVDKVKECVDHLSRDDEDEEKLVASLWGAERCLRVLESVTVHNPENQSYLIAYKDSQLIISSAKALQHCEDLIQQYNRAENSICVADSNPLPYQNVTNHVGKAVEDCMRAIIGVLLNLTNDNEWGSTKTGEQEGLIGTAMNCVLQVPKYLPQEQRFDIRVLGLGLLINLVEYSARNRHCLVNMQTSCSFDSSFSSGEGDHSLRLAGQVHAVQALVQLFLERERAAQLAESKTDELIKDAPTTQHDKSGEWQETSGEIQWVSTEKTDGAEEKQKKEEEDEELDLNKALQHAGKHMEDCIVASYTALLLGCLCQESPINVTTVREYLPEGDFSIMTEMLKKFLSFMNLTCAVGTTGQKSISRVIEYLEHC

Target ORF information:

RefSeq Version	NM_001301330.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus WAPL cohesin release factor (Wapl), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001301330.1

ORF Insert Sequence:

ATGACATCCA GATTTGGAAA AACTTACAGT AGGAAAGGAG GAAATGGCAG TTCAAAATTT 
GATGAAGTTT TTTCCAACAA ACGGACTACT CTTAGTACAA AATGGGGTGA GACCACATTT 
ATGGCTAAAT TAGGGCAGAA GAGGCCCAAT TTCAAACCAG ATATTCAAGA AATTCCGAAG 
AAACCTAAAG TAGAAGAAGA AGATACTGGA GATCCCTTTG GTTTTGATAG TGATGATGAG 
TCTCTACCTG TTTCTTCAAA AAATTTAGCC CAGGGTAAGG GTTCATCTTA CTCAGAATCT 
AGTGAGGCTG CTCAGCTGGA AGAAGTCACT TCTGTATTTG AAGCTAATAG CAAATGTAGT 
CATGTGGTGG GTGAAGACAG TTTTGCTTCC GACAGATGCT TACTTGTGGA GGATACTTTA 
ATTGGGAAAG AGAAGAGCAT AAGTAGAATT CCAGAAGACA ACGCAAACAA AAGTAGTTGC 
ACTAAGTTGC TAACTTCAGA TAAAGTGGAG AATTTTAGTG AAGAACATGA AAAAAATAGT 
CACCACTTTC ACAAAAATGC TGAAGATAGT ACTAAGAAAC CCAATGCAGA AACCGCAGTG 
GCTTCTGAAT ATAAAGCTGA TGAAACTAAA GAAACAAATG ATACTTGGAA CTCCCAGTCT 
GGAAAAAGAA CAGAGTCTCC ATCTGAAAGT TGTCCAGTCA AAGGATCTGT AAGAACTGGT 
TTATATGAAT GGGATAATGA TTTTGAAGAT ATCAGGTCAG AAGACTGTAT TTTAAGTTTG 
GATAATGAGT CTCTTTTGGA GATGAAAGAC GAGGATTTAA AAAATCGGAT TGGAGGATTG 
GAAAATCTAA ATGAAACCTT TGAAGAAGAT ATCATACAAA GTGTTCTTAG GCCAAGCAAC 
TGTAGGACGT ACTGTAGGGC CAATAAAGCG AGATCCTCAC AGGGAGCATC AAATTTTGAT 
AAGCTAATGG ATGGCACCAG TCAGTCCTTA GCCAAAGCAA ACAGTGAATC AAGTAAAGAT 
GGCCTGAATC AGGCAAAGAA AGGTAGTGCA AGTTGTGGGA CCAGTTTTCG AGGAACAGTT 
GGACGGACTA GAGATTACAC TGTTTTACAT CCATCTTGCT TGTCAGTGTG TAATGTTACC 
ATCCAGGATA CTATGGAACG GAGTATGGAT GAGTTCACCG CATCCACTCC TGCAGATTTA 
GGAGAGGCTG GCCGGCTCAG AAAAAAGGCA GATATTGCAA CCTCCAAGAC CACTACTAGA 
TTTCGACCTA GTAATACTAA ATCCAAAAAG GATGTTAAAC TTGAATTTTT TGGTTTTGAA 
GATCATGATG AGACAGGAGG TGATGAAGGG GGTTCTGGAA GTTCTAATTA CAAAATTAAA 
TATTTTGGCT TTGACGATCT CAGCGAAAGT GAAGATGATG ATGATGACGA CTGTCAAGTG 
GAAAGAAAGA AAGACAAAAA AAGAACTAAA ACAGCTCCAT CACCTTCCCA GCAGCCTCCT 
CCTGAAAGCA GCGACAATTC CCAGGATAGT CAGTCTAGTA CTAATAATGC AGAGGACTTG 
CCTGGTGTGC CTGAGAGTGT GAAGAAGCCC ATAAGTAAAC AAGGAGATAA ATCCAAGGAA 
AATACCAGAA AGATTTTTAG TGGCCCCAAA CGGTCACCTA CAAAAGCTGT ATATAATGCC 
AGGCATTGGA ACCATCCAGA CTCGGAAGAA TTGCCTGGAC CACCAATAGC AAAACCTCAG 
CGTGTCACAG TGAGGCTGTC TTCAAAGGAA CCAAATCAAA AAGATGATGG AGTTTTTAAG 
GCTCCTGCAC CACCACTCAA AGTGATAAAA ACTGTGACAA TACCTACTCA GCCCTACCAA 
GAAATAGTTA CTGCACTGAA ATGCAGAAAA GAAGACAAAG AATTATATAC TGTTGTTCAG 
CACGTGAAAC ACTTCAATGA TGTGGTGGAA TTTGGTGAAA ATCAAGAGTT CACTGATGAC 
ATTGAATACT TGTTAAGTGG CTTAAAGAGT ACTCAGCCTC TAAACACACG TTGCCTTAGT 
GTTATCAGCT TAGCTACTAA ATGTGCCATG CCCAGTTTTC GGATGCATCT GAGGGCACAT 
GGGATGGTTG CAATGGTCTT TAAAACTTTG GATGATTCCC AGCATCATCA GAATCTGTCC 
CTCTGTACAG CTGCTCTCAT GTACATATTG AGTAGAGACC GTTTGAACAT GGATCTTGAT 
AGGGCCAGCC TAGATCTCAT GATTCGGCTT TTGGAGTTGG AACAAGATGC CTCTTCAGCT 
AAGCTACTGA ATGAAAAAGA CATGAACAAG ATCAAAGAAA AGATCCGAAG ACTCTGTGAA 
ACTGTGCACA ACAAGCATCT TGATCTAGAA AACATAACGA CTGGTCATTT AGCTATGGAG 
ACATTGCTGT CCCTCACTTC CAAACGAGCA GGAGATTGGT TTAAAGAAGA GCTCCGACTT 
CTGGGTGGTC TGGATCATAT TGTAGATAAA GTAAAAGAGT GTGTGGATCA TTTAAGTAGA 
GATGATGAGG ACGAAGAGAA ACTAGTAGCC TCATTATGGG GAGCAGAGAG ATGTTTACGA 
GTTTTAGAGA GTGTAACAGT GCATAATCCA GAGAATCAAA GCTACTTGAT AGCCTATAAA 
GATTCACAAC TCATTATTTC ATCAGCTAAA GCATTACAGC ATTGTGAAGA CCTGATTCAG 
CAGTACAACC GTGCTGAGAA CAGCATCTGT GTAGCAGACA GTAACCCTCT GCCTTACCAG 
AATGTAACTA ACCATGTGGG CAAAGCAGTG GAGGACTGCA TGAGGGCTAT AATTGGAGTA 
TTGCTCAATT TAACTAATGA TAATGAGTGG GGCAGCACAA AGACAGGAGA ACAAGAAGGA 
CTCATAGGCA CAGCGATGAA CTGTGTTCTT CAGGTTCCAA AGTACCTACC TCAGGAGCAG 
AGATTTGATA TTCGAGTGCT GGGATTGGGT CTACTCATAA ACCTGGTGGA GTATAGTGCC 
CGGAATCGAC ACTGCCTTGT CAACATGCAA ACATCCTGTT CCTTTGATTC CTCCTTCTCT 
AGTGGAGAAG GCGATCATAG TTTAAGGCTA GCCGGACAAG TTCATGCTGT TCAAGCTTTA 
GTGCAGCTAT TTCTCGAACG AGAGAGAGCA GCACAATTGG CAGAAAGTAA AACAGATGAA 
TTGATTAAAG ATGCTCCTAC CACTCAGCAT GATAAGAGTG GAGAGTGGCA AGAAACAAGT 
GGAGAAATAC AGTGGGTATC AACTGAAAAG ACTGATGGTG CAGAGGAGAA GCAGAAGAAG 
GAGGAGGAGG ATGAAGAACT TGACCTCAAT AAAGCCCTTC AGCATGCTGG CAAACACATG 
GAGGATTGCA TCGTAGCCTC CTACACAGCC CTGCTTCTTG GGTGTCTCTG CCAGGAAAGT 
CCAATCAATG TAACTACAGT AAGGGAATAT CTTCCAGAAG GAGATTTCTC CATAATGACA 
GAGATGCTTA AAAAGTTCTT AAGCTTCATG AATCTTACGT GTGCTGTTGG AACAACAGGC 
CAGAAGTCTA TCTCTAGAGT GATTGAATAT TTGGAACATT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Wapl ( XM_006518824.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006518887.1 Mus musculus WAPL cohesin release factor (Wapl), transcript variant X1, mRNA.

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CloneID	OMu49859
Clone ID Related Accession (Same CDS sequence)	XM_006518824.3 , XM_006518824.4
Accession Version	XM_006518824.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	wings apart-like protein homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006518824.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACATCCA GATTTGGAAA AACTTACAGT AGGAAAGGAG GAAATGGCAG TTCAAAATTT 
GATGAAGTTT TTTCCAACAA ACGGACTACT CTTAGTACAA AATGGGGTGA GACCACATTT 
ATGGCTAAAT TAGGGCAGAA GAGGCCCAAT TTCAAACCAG ATATTCAAGA AATTCCGAAG 
AAACCTAAAG TAGAAGAAGA AGATACTGGA GATCCCTTTG GTTTTGATAG TGATGATGAG 
TCTCTACCTG TTTCTTCAAA AAATTTAGCC CAGGGTAAGG GTTCATCTTA CTCAGAATCT 
AGTGAGGCTG CTCAGCTGGA AGAAGTCACT TCTGTATTTG AAGCTAATAG CAAATGTAGT 
CATGTGGTGG GTGAAGACAG TTTTGCTTCC GACAGATGCT TACTTGTGGA GGATACTTTA 
ATTGGGAAAG AGAAGAGCAT AAGTAGAATT CCAGAAGACA ACGCAAACAA AAGTAGTTGC 
ACTAAGTTGC TAACTTCAGA TAAAGTGGAG AATTTTAGTG AAGAACATGA AAAAAATAGT 
CACCACTTTC ACAAAAATGC TGAAGATAGT ACTAAGAAAC CCAATGCAGA AACCGCAGTG 
GCTTCTGAAT ATAAAGCTGA TGAAACTAAA GAAACAAATG ATACTTGGAA CTCCCAGTCT 
GGAAAAAGAA CAGAGTCTCC ATCTGAAAGT TGTCCAGTCA AAGGATCTGT AAGAACTGGT 
TTATATGAAT GGGATAATGA TTTTGAAGAT ATCAGGTCAG AAGACTGTAT TTTAAGTTTG 
GATAATGAGT CTCTTTTGGA GATGAAAGAC GAGGATTTAA AAAATCGGAT TGGAGGATTG 
GAAAATCTAA ATGAAACCTT TGAAGAAGAT ATCATACAAA GTGTTCTTAG GCCAAGCAAC 
TGTAGGACGT ACTGTAGGGC CAATAAAGCG AGATCCTCAC AGGGAGCATC AAATTTTGAT 
AAGCTAATGG ATGGCACCAG TCAGTCCTTA GCCAAAGCAA ACAGTGAATC AAGTAAAGAT 
GGCCTGAATC AGGCAAAGAA AGGTAGTGCA AGTTGTGGGA CCAGTTTTCG AGGAACAGTT 
GGACGGACTA GAGATTACAC TGTTTTACAT CCATCTTGCT TGTCAGTGTG TAATGTTACC 
ATCCAGGATA CTATGGAACG GAGTATGGAT GAGTTCACCG CATCCACTCC TGCAGATTTA 
GGAGAGGCTG GCCGGCTCAG AAAAAAGGCA GATATTGCAA CCTCCAAGAC CACTACTAGA 
TTTCGACCTA GTAATACTAA ATCCAAAAAG GATGTTAAAC TTGAATTTTT TGGTTTTGAA 
GATCATGATG AGACAGGAGG TGATGAAGGG GGTTCTGGAA GTTCTAATTA CAAAATTAAA 
TATTTTGGCT TTGACGATCT CAGCGAAAGT GAAGATGATG ATGATGACGA CTGTCAAGTG 
GAAAGAAAGA AAGACAAAAA AAGAACTAAA ACAGCTCCAT CACCTTCCCA GCAGCCTCCT 
CCTGAAAGCA GCGACAATTC CCAGGATAGT CAGTCTAGTA CTAATAATGC AGAAAACTTG 
GATTTTACAG AGGACTTGCC TGGTGTGCCT GAGAGTGTGA AGAAGCCCAT AAGTAAACAA 
GGAGATAAAT CCAAGGAAAA TACCAGAAAG ATTTTTAGTG GCCCCAAACG GTCACCTACA 
AAAGCTGTAT ATAATGCCAG GCATTGGAAC CATCCAGACT CGGAAGAATT GCCTGGACCA 
CCAATAGCAA AACCTCAGCG TGTCACATTA TATACTGTTG TTCAGCACGT GAAACACTTC 
AATGATGTGG TGGAATTTGG TGAAAATCAA GAGTTCACTG ATGACATTGA ATACTTGTTA 
AGTGGCTTAA AGAGTACTCA GCCTCTAAAC ACACGTTGCC TTAGTGTTAT CAGCTTAGCT 
ACTAAATGTG CCATGCCCAG TTTTCGGATG CATCTGAGGG CACATGGGAT GGTTGCAATG 
GTCTTTAAAA CTTTGGATGA TTCCCAGCAT CATCAGAATC TGTCCCTCTG TACAGCTGCT 
CTCATGTACA TATTGAGTAG AGACCGTTTG AACATGGATC TTGATAGGGC CAGCCTAGAT 
CTCATGATTC GGCTTTTGGA GTTGGAACAA GATGCCTCTT CAGCTAAGCT ACTGAATGAA 
AAAGACATGA ACAAGATCAA AGAAAAGATC CGAAGACTCT GTGAAACTGT GCACAACAAG 
CATCTTGATC TAGAAAACAT AACGACTGGT CATTTAGCTA TGGAGACATT GCTGTCCCTC 
ACTTCCAAAC GAGCAGGAGA TTGGTTTAAA GAAGAGCTCC GACTTCTGGG TGGTCTGGAT 
CATATTGTAG ATAAAGTAAA AGAGTGTGTG GATCATTTAA GTAGAGATGA TGAGGACGAA 
GAGAAACTAG TAGCCTCATT ATGGGGAGCA GAGAGATGTT TACGAGTTTT AGAGAGTGTA 
ACAGTGCATA ATCCAGAGAA TCAAAGCTAC TTGATAGCCT ATAAAGATTC ACAACTCATT 
ATTTCATCAG CTAAAGCATT ACAGCATTGT GAAGACCTGA TTCAGCAGTA CAACCGTGCT 
GAGAACAGCA TCTGTGTAGC AGACAGTAAC CCTCTGCCTT ACCAGAATGT AACTAACCAT 
GTGGGCAAAG CAGTGGAGGA CTGCATGAGG GCTATAATTG GAGTATTGCT CAATTTAACT 
AATGATAATG AGTGGGGCAG CACAAAGACA GGAGAACAAG AAGGACTCAT AGGCACAGCG 
ATGAACTGTG TTCTTCAGGT TCCAAAGTAC CTACCTCAGG AGCAGAGATT TGATATTCGA 
GTGCTGGGAT TGGGTCTACT CATAAACCTG GTGGAGTATA GTGCCCGGAA TCGACACTGC 
CTTGTCAACA TGCAAACATC CTGTTCCTTT GATTCCTCCT TCTCTAGTGG AGAAGGCGAT 
CATAGTTTAA GGCTAGCCGG ACAAGTTCAT GCTGTTCAAG CTTTAGTGCA GCTATTTCTC 
GAACGAGAGA GAGCAGCACA ATTGGCAGAA AGTAAAACAG ATGAATTGAT TAAAGATGCT 
CCTACCACTC AGCATGATAA GAGTGGAGAG TGGCAAGAAA CAAGTGGAGA AATACAGTGG 
GTATCAACTG AAAAGACTGA TGGTGCAGAG GAGAAGCAGA AGAAGGAGGA GGAGGATGAA 
GAACTTGACC TCAATAAAGC CCTTCAGCAT GCTGGCAAAC ACATGGAGGA TTGCATCGTA 
GCCTCCTACA CAGCCCTGCT TCTTGGGTGT CTCTGCCAGG AAAGTCCAAT CAATGTAACT 
ACAGTAAGGG AATATCTTCC AGAAGGAGAT TTCTCCATAA TGACAGAGAT GCTTAAAAAG 
TTCTTAAGCT TCATGAATCT TACGTGTGCT GTTGGAACAA CAGGCCAGAA GTCTATCTCT 
AGAGTGATTG AATATTTGGA ACATTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006518887.1
CDS	591..4040
Translation	MTSRFGKTYSRKGGNGSSKFDEVFSNKRTTLSTKWGETTFMAKLGQKRPNFKPDIQEIPKKPKVEEEDTGDPFGFDSDDESLPVSSKNLAQGKGSSYSESSEAAQLEEVTSVFEANSKCSHVVGEDSFASDRCLLVEDTLIGKEKSISRIPEDNANKSSCTKLLTSDKVENFSEEHEKNSHHFHKNAEDSTKKPNAETAVASEYKADETKETNDTWNSQSGKRTESPSESCPVKGSVRTGLYEWDNDFEDIRSEDCILSLDNESLLEMKDEDLKNRIGGLENLNETFEEDIIQSVLRPSNCRTYCRANKARSSQGASNFDKLMDGTSQSLAKANSESSKDGLNQAKKGSASCGTSFRGTVGRTRDYTVLHPSCLSVCNVTIQDTMERSMDEFTASTPADLGEAGRLRKKADIATSKTTTRFRPSNTKSKKDVKLEFFGFEDHDETGGDEGGSGSSNYKIKYFGFDDLSESEDDDDDDCQVERKKDKKRTKTAPSPSQQPPPESSDNSQDSQSSTNNAENLDFTEDLPGVPESVKKPISKQGDKSKENTRKIFSGPKRSPTKAVYNARHWNHPDSEELPGPPIAKPQRVTLYTVVQHVKHFNDVVEFGENQEFTDDIEYLLSGLKSTQPLNTRCLSVISLATKCAMPSFRMHLRAHGMVAMVFKTLDDSQHHQNLSLCTAALMYILSRDRLNMDLDRASLDLMIRLLELEQDASSAKLLNEKDMNKIKEKIRRLCETVHNKHLDLENITTGHLAMETLLSLTSKRAGDWFKEELRLLGGLDHIVDKVKECVDHLSRDDEDEEKLVASLWGAERCLRVLESVTVHNPENQSYLIAYKDSQLIISSAKALQHCEDLIQQYNRAENSICVADSNPLPYQNVTNHVGKAVEDCMRAIIGVLLNLTNDNEWGSTKTGEQEGLIGTAMNCVLQVPKYLPQEQRFDIRVLGLGLLINLVEYSARNRHCLVNMQTSCSFDSSFSSGEGDHSLRLAGQVHAVQALVQLFLERERAAQLAESKTDELIKDAPTTQHDKSGEWQETSGEIQWVSTEKTDGAEEKQKKEEEDEELDLNKALQHAGKHMEDCIVASYTALLLGCLCQESPINVTTVREYLPEGDFSIMTEMLKKFLSFMNLTCAVGTTGQKSISRVIEYLEHC

Target ORF information:

RefSeq Version	XM_006518824.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus WAPL cohesin release factor (Wapl), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006518824.3

ORF Insert Sequence:

ATGACATCCA GATTTGGAAA AACTTACAGT AGGAAAGGAG GAAATGGCAG TTCAAAATTT 
GATGAAGTTT TTTCCAACAA ACGGACTACT CTTAGTACAA AATGGGGTGA GACCACATTT 
ATGGCTAAAT TAGGGCAGAA GAGGCCCAAT TTCAAACCAG ATATTCAAGA AATTCCGAAG 
AAACCTAAAG TAGAAGAAGA AGATACTGGA GATCCCTTTG GTTTTGATAG TGATGATGAG 
TCTCTACCTG TTTCTTCAAA AAATTTAGCC CAGGGTAAGG GTTCATCTTA CTCAGAATCT 
AGTGAGGCTG CTCAGCTGGA AGAAGTCACT TCTGTATTTG AAGCTAATAG CAAATGTAGT 
CATGTGGTGG GTGAAGACAG TTTTGCTTCC GACAGATGCT TACTTGTGGA GGATACTTTA 
ATTGGGAAAG AGAAGAGCAT AAGTAGAATT CCAGAAGACA ACGCAAACAA AAGTAGTTGC 
ACTAAGTTGC TAACTTCAGA TAAAGTGGAG AATTTTAGTG AAGAACATGA AAAAAATAGT 
CACCACTTTC ACAAAAATGC TGAAGATAGT ACTAAGAAAC CCAATGCAGA AACCGCAGTG 
GCTTCTGAAT ATAAAGCTGA TGAAACTAAA GAAACAAATG ATACTTGGAA CTCCCAGTCT 
GGAAAAAGAA CAGAGTCTCC ATCTGAAAGT TGTCCAGTCA AAGGATCTGT AAGAACTGGT 
TTATATGAAT GGGATAATGA TTTTGAAGAT ATCAGGTCAG AAGACTGTAT TTTAAGTTTG 
GATAATGAGT CTCTTTTGGA GATGAAAGAC GAGGATTTAA AAAATCGGAT TGGAGGATTG 
GAAAATCTAA ATGAAACCTT TGAAGAAGAT ATCATACAAA GTGTTCTTAG GCCAAGCAAC 
TGTAGGACGT ACTGTAGGGC CAATAAAGCG AGATCCTCAC AGGGAGCATC AAATTTTGAT 
AAGCTAATGG ATGGCACCAG TCAGTCCTTA GCCAAAGCAA ACAGTGAATC AAGTAAAGAT 
GGCCTGAATC AGGCAAAGAA AGGTAGTGCA AGTTGTGGGA CCAGTTTTCG AGGAACAGTT 
GGACGGACTA GAGATTACAC TGTTTTACAT CCATCTTGCT TGTCAGTGTG TAATGTTACC 
ATCCAGGATA CTATGGAACG GAGTATGGAT GAGTTCACCG CATCCACTCC TGCAGATTTA 
GGAGAGGCTG GCCGGCTCAG AAAAAAGGCA GATATTGCAA CCTCCAAGAC CACTACTAGA 
TTTCGACCTA GTAATACTAA ATCCAAAAAG GATGTTAAAC TTGAATTTTT TGGTTTTGAA 
GATCATGATG AGACAGGAGG TGATGAAGGG GGTTCTGGAA GTTCTAATTA CAAAATTAAA 
TATTTTGGCT TTGACGATCT CAGCGAAAGT GAAGATGATG ATGATGACGA CTGTCAAGTG 
GAAAGAAAGA AAGACAAAAA AAGAACTAAA ACAGCTCCAT CACCTTCCCA GCAGCCTCCT 
CCTGAAAGCA GCGACAATTC CCAGGATAGT CAGTCTAGTA CTAATAATGC AGAAAACTTG 
GATTTTACAG AGGACTTGCC TGGTGTGCCT GAGAGTGTGA AGAAGCCCAT AAGTAAACAA 
GGAGATAAAT CCAAGGAAAA TACCAGAAAG ATTTTTAGTG GCCCCAAACG GTCACCTACA 
AAAGCTGTAT ATAATGCCAG GCATTGGAAC CATCCAGACT CGGAAGAATT GCCTGGACCA 
CCAATAGCAA AACCTCAGCG TGTCACATTA TATACTGTTG TTCAGCACGT GAAACACTTC 
AATGATGTGG TGGAATTTGG TGAAAATCAA GAGTTCACTG ATGACATTGA ATACTTGTTA 
AGTGGCTTAA AGAGTACTCA GCCTCTAAAC ACACGTTGCC TTAGTGTTAT CAGCTTAGCT 
ACTAAATGTG CCATGCCCAG TTTTCGGATG CATCTGAGGG CACATGGGAT GGTTGCAATG 
GTCTTTAAAA CTTTGGATGA TTCCCAGCAT CATCAGAATC TGTCCCTCTG TACAGCTGCT 
CTCATGTACA TATTGAGTAG AGACCGTTTG AACATGGATC TTGATAGGGC CAGCCTAGAT 
CTCATGATTC GGCTTTTGGA GTTGGAACAA GATGCCTCTT CAGCTAAGCT ACTGAATGAA 
AAAGACATGA ACAAGATCAA AGAAAAGATC CGAAGACTCT GTGAAACTGT GCACAACAAG 
CATCTTGATC TAGAAAACAT AACGACTGGT CATTTAGCTA TGGAGACATT GCTGTCCCTC 
ACTTCCAAAC GAGCAGGAGA TTGGTTTAAA GAAGAGCTCC GACTTCTGGG TGGTCTGGAT 
CATATTGTAG ATAAAGTAAA AGAGTGTGTG GATCATTTAA GTAGAGATGA TGAGGACGAA 
GAGAAACTAG TAGCCTCATT ATGGGGAGCA GAGAGATGTT TACGAGTTTT AGAGAGTGTA 
ACAGTGCATA ATCCAGAGAA TCAAAGCTAC TTGATAGCCT ATAAAGATTC ACAACTCATT 
ATTTCATCAG CTAAAGCATT ACAGCATTGT GAAGACCTGA TTCAGCAGTA CAACCGTGCT 
GAGAACAGCA TCTGTGTAGC AGACAGTAAC CCTCTGCCTT ACCAGAATGT AACTAACCAT 
GTGGGCAAAG CAGTGGAGGA CTGCATGAGG GCTATAATTG GAGTATTGCT CAATTTAACT 
AATGATAATG AGTGGGGCAG CACAAAGACA GGAGAACAAG AAGGACTCAT AGGCACAGCG 
ATGAACTGTG TTCTTCAGGT TCCAAAGTAC CTACCTCAGG AGCAGAGATT TGATATTCGA 
GTGCTGGGAT TGGGTCTACT CATAAACCTG GTGGAGTATA GTGCCCGGAA TCGACACTGC 
CTTGTCAACA TGCAAACATC CTGTTCCTTT GATTCCTCCT TCTCTAGTGG AGAAGGCGAT 
CATAGTTTAA GGCTAGCCGG ACAAGTTCAT GCTGTTCAAG CTTTAGTGCA GCTATTTCTC 
GAACGAGAGA GAGCAGCACA ATTGGCAGAA AGTAAAACAG ATGAATTGAT TAAAGATGCT 
CCTACCACTC AGCATGATAA GAGTGGAGAG TGGCAAGAAA CAAGTGGAGA AATACAGTGG 
GTATCAACTG AAAAGACTGA TGGTGCAGAG GAGAAGCAGA AGAAGGAGGA GGAGGATGAA 
GAACTTGACC TCAATAAAGC CCTTCAGCAT GCTGGCAAAC ACATGGAGGA TTGCATCGTA 
GCCTCCTACA CAGCCCTGCT TCTTGGGTGT CTCTGCCAGG AAAGTCCAAT CAATGTAACT 
ACAGTAAGGG AATATCTTCC AGAAGGAGAT TTCTCCATAA TGACAGAGAT GCTTAAAAAG 
TTCTTAAGCT TCATGAATCT TACGTGTGCT GTTGGAACAA CAGGCCAGAA GTCTATCTCT 
AGAGTGATTG AATATTTGGA ACATTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A mechanism of cohesin-dependent loop extrusion organizes zygotic genome architecture.
The EMBO journal36(24)3600-3618(2017 12)
Gassler J,Brand?o HB,Imakaev M,Flyamer IM,Ladst?tter S,Bickmore WA,Peters JM,Mirny LA,Tachibana K

Cohesin is positioned in mammalian genomes by transcription, CTCF and Wapl.
Nature544(7651)503-507(2017 04)
Busslinger GA,Stocsits RR,van der Lelij P,Axelsson E,Tedeschi A,Galjart N,Peters JM

Cohesin Removal along the Chromosome Arms during the First Meiotic Division Depends on a NEK1-PP1?-WAPL Axis in the Mouse.
Cell reports17(4)977-986(2016 10)
Brie?o-Enr?quez MA,Moak SL,Toledo M,Filter JJ,Gray S,Barbero JL,Cohen PE,Holloway JK

Allogeneic T cell responses are regulated by a specific miRNA-mRNA network.
The Journal of clinical investigation123(11)4739-54(2013 Nov)
Sun Y,Tawara I,Zhao M,Qin ZS,Toubai T,Mathewson N,Tamaki H,Nieves E,Chinnaiyan AM,Reddy P

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Wapl cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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