Tkt cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tkt
Entrez Gene ID	21881
Full Name	transketolase
Synonyms	p68
General protein information	Preferred Names transketolase Names transketolase TK
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 14 Map Location: 14\|14 B
Summary	This gene encodes an enzyme that binds magnesium and thiamine pyrophosphate and catalyzes the transfer of sugar phosphates to an aldose acceptor. This enzyme is a key component of the pentose phosphate pathway during glycolysis. It is significantly expressed in the cornea and may be involved in the cellular response against oxidative stress. Haploinsufficiency of this gene leads to decreased growth and reduction of adipose tissue. [provided by RefSeq, Dec 2013].

mRNA and Protein(s)

mRNA	Protein	Name
NM_009388.6	NP_033414.1	transketolase

Pathways from BioSystems

Homologies

Homo sapiens (human)	TKT	NP_001244957.1
Danio rerio (zebrafish)	LOC557518	XP_005155944.1
Canis lupus familiaris (dog)	TKT	XP_005632478.1
Bos taurus (cattle)	TKT	NP_001003906.1
Gallus gallus (chicken)	TKT	XP_004944618.1
Pan troglodytes (chimpanzee)	TKT	NP_001009097.1
Mus musculus (house mouse)	Tkt	NP_033414.1
Rattus norvegicus (Norway rat)	Tkt	NP_072114.1
Xenopus tropicalis (tropical clawed frog)	tkt	NP_001015866.1

Gene Ontology
Bibliography

Related articles in PubMed

Identification of transketolase as a target of PARIS in substantia nigra.
Kim H, Kang H, Lee Y, Park CH, Jo A, Khang R, Shin JH
Biochemical and biophysical research communications493(2)1050-1056(2017 11)

Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to Mouse Embryo Mesoderm Development.
Bulusu V, Prior N, Snaebjornsson MT, Kuehne A, Sonnen KF, Kress J, Stein F, Schultz C, Sauer U, Aulehla A
Developmental cell40(4)331-341.e4(2017 02)

Requirement for the Mitochondrial Pyruvate Carrier in Mammalian Development Revealed by a Hypomorphic Allelic Series.
Bowman CE, Zhao L, Hartung T, Wolfgang MJ
Molecular and cellular biology36(15)2089-104(2016 Aug)

Comparative proteomic profiling reveals aberrant cell proliferation in the brain of embryonic Ts1Cje, a mouse model of Down syndrome.
Ishihara K, Kanai S, Sago H, Yamakawa K, Akiba S
Neuroscience2811-15(2014 Dec)

Decreased transketolase activity contributes to impaired hippocampal neurogenesis induced by thiamine deficiency.
Zhao Y, Pan X, Zhao J, Wang Y, Peng Y, Zhong C
Journal of neurochemistry111(2)537-46(2009 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Zinc finger protein 746 overexpression suppresses TKT transcription via IRS-like motif in the TKT promoter.
Title: Identification of transketolase as a target of PARIS in substantia nigra.

decreased TK activity leads to pentose-phosphate pathway dysfunction and contributes to impaired hippocampal neurogenesis induced by thiamine deficiency
Title: Decreased transketolase activity contributes to impaired hippocampal neurogenesis induced by thiamine deficiency.

The following Tkt gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tkt cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu16587	NM_009388.6 Latest version!	Mus musculus transketolase (Tkt), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$370.30 $529.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tkt ( NM_009388.6 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033414.1 Mus musculus transketolase (Tkt), mRNA.

Price & Availability↑

CloneID	OMu16587
Clone ID Related Accession (Same CDS sequence)	NM_009388.6
Accession Version	NM_009388.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Mus musculus(house mouse)
Product	transketolase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY276711.1, AK166763.1, AK030446.1, AC154646.2 and AI837706.1. On Dec 18, 2013 this sequence version replaced NM_009388.5. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK030446.1, AK166763.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGAAGGTT ACCATAAGCC AGATCAGCAG AAGCTCCAGG CCCTGAAGGA CACAGCCAAT 
CGCCTGCGCA TCAGCTCCAT CCAGGCCACC ACCGCGGCAG GCTCAGGCCA CCCCACATCA 
TGCTGCAGCG CTGCCGAGAT CATGGCTGTC CTGTTTTTCC ATACCATGCG TTACAAGGCC 
CTGGATCCCC GAAACCCTCA CAATGATCGA TTTGTGCTCT CTAAGGGCCA TGCAGCTCCC 
ATTTTATATG CAGTCTGGGC TGAAGCTGGC TTCCTACCCG AGGCCGAGCT GCTGAACCTG 
AGGAAGATCA GCTCTGACTT GGACGGGCAT CCTGTCCCGA AACAAGCCTT CACCGATGTG 
GCCACTGGCT CCCTGGGCCA GGGCCTGGGA GCTGCTTGCG GGATGGCATA CACAGGCAAA 
TACTTTGACA AAGCCAGCTA CCGAGTCTAT TGCATGCTGG GAGACGGGGA GGTCTCCGAG 
GGCTCCGTCT GGGAGGCCAT GGCCTTTGCT GGAATTTACA AGCTGGACAA CCTCGTTGCC 
ATTTTTGACA TCAACCGCCT GGGCCAGAGC GACCCAGCCC CGCTGCAGCA CCAGGTGGAC 
ATCTACCAGA AGCGCTGTGA GGCCTTTGGC TGGCACACCA TCATCGTGGA CGGACACAGC 
GTGGAGGAGC TGTGCAAGGC CTTTGGTCAG GCCAAGCACC AACCAACAGC CATCATTGCC 
AAGACCTTCA AGGGCCGAGG GATCACAGGG ATTGAAGACA AGGAGGCGTG GCACGGGAAG 
CCCCTCCCCA AAAACATGGC CGAGCAGATT ATCCAGGAGA TTTACAGCCA GGTTCAGAGC 
AAAAAGAAGA TCCTGGCCAC GCCCCCTCAG GAGGATGCCC CATCCGTGGA CATTGCTAAC 
ATCCGAATGC CTACGCCACC CAGCTACAAA GTGGGGGACA AGATAGCCAC CCGGAAGGCC 
TATGGACTGG CCCTCGCTAA GCTGGGCCAC GCCAGTGACC GTATCATTGC CCTGGATGGA 
GACACCAAGA ATTCCACCTT CTCGGAGCTC TTCAAAAAGG AGCACCCAGA CCGGTTCATT 
GAGTGCTACA TTGCCGAGCA AAACATGGTG AGCATTGCCG TGGGCTGTGC CACACGTGAC 
CGGACAGTGC CCTTCTGCAG TACTTTCGCG GCCTTCTTCA CACGGGCCTT CGACCAGATT 
CGCATGGCCG CCATCTCTGA GAGCAACATC AACCTCTGTG GCTCCCACTG TGGTGTGTCC 
ATTGGGGAAG ACGGGCCCTC TCAGATGGCC CTCGAAGACC TGGCCATGTT CCGGTCAGTC 
CCCATGTCCA CCGTCTTTTA CCCAAGCGAT GGAGTTGCAA CAGAGAAGGC AGTGGAGTTA 
GCAGCCAACA CAAAGGGCAT TTGCTTCATC CGGACCAGCC GCCCAGAGAA TGCCATTATT 
TATAGCAACA ATGAGGATTT CCAGGTCGGC CAAGCCAAGG TGGTCCTGAA GAGCAAGGAT 
GACCAAGTGA CAGTGATCGG GGCTGGTGTA ACTCTGCATG AGGCCTTGGC TGCTGCAGAG 
AGTCTAAAGA AAGATAAGAT CAGCATCCGG GTGCTGGATC CCTTCACTAT CAAGCCCCTG 
GACAGGAAAC TCATCCTAGA CTCTGCCCGA GCAACCAAAG GCAGGATCCT CACCGTGGAG 
GACCACTACT ACGAAGGTGG CATAGGAGAG GCAGTGTCTG CTGCCGTAGT GGGTGAACCT 
GGAGTGACGG TCACTCGCCT GGCTGTCAGC CAAGTACCAC GAAGTGGCAA GCCAGCTGAG 
CTACTGAAGA TGTTCGGTAT TGACAAGGAC GCCATTGTGC AAGCTGTGAA AGGCCTTGTC 
ACCAAGGGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033414.1
CDS	144..2015
Translation	MEGYHKPDQQKLQALKDTANRLRISSIQATTAAGSGHPTSCCSAAEIMAVLFFHTMRYKALDPRNPHNDRFVLSKGHAAPILYAVWAEAGFLPEAELLNLRKISSDLDGHPVPKQAFTDVATGSLGQGLGAACGMAYTGKYFDKASYRVYCMLGDGEVSEGSVWEAMAFAGIYKLDNLVAIFDINRLGQSDPAPLQHQVDIYQKRCEAFGWHTIIVDGHSVEELCKAFGQAKHQPTAIIAKTFKGRGITGIEDKEAWHGKPLPKNMAEQIIQEIYSQVQSKKKILATPPQEDAPSVDIANIRMPTPPSYKVGDKIATRKAYGLALAKLGHASDRIIALDGDTKNSTFSELFKKEHPDRFIECYIAEQNMVSIAVGCATRDRTVPFCSTFAAFFTRAFDQIRMAAISESNINLCGSHCGVSIGEDGPSQMALEDLAMFRSVPMSTVFYPSDGVATEKAVELAANTKGICFIRTSRPENAIIYSNNEDFQVGQAKVVLKSKDDQVTVIGAGVTLHEALAAAESLKKDKISIRVLDPFTIKPLDRKLILDSARATKGRILTVEDHYYEGGIGEAVSAAVVGEPGVTVTRLAVSQVPRSGKPAELLKMFGIDKDAIVQAVKGLVTKG

Target ORF information:

RefSeq Version	NM_009388.6
Organism	Mus musculus(house mouse)
Definition	Mus musculus transketolase (Tkt), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009388.6

ORF Insert Sequence:

ATGGAAGGTT ACCATAAGCC AGATCAGCAG AAGCTCCAGG CCCTGAAGGA CACAGCCAAT 
CGCCTGCGCA TCAGCTCCAT CCAGGCCACC ACCGCGGCAG GCTCAGGCCA CCCCACATCA 
TGCTGCAGCG CTGCCGAGAT CATGGCTGTC CTGTTTTTCC ATACCATGCG TTACAAGGCC 
CTGGATCCCC GAAACCCTCA CAATGATCGA TTTGTGCTCT CTAAGGGCCA TGCAGCTCCC 
ATTTTATATG CAGTCTGGGC TGAAGCTGGC TTCCTACCCG AGGCCGAGCT GCTGAACCTG 
AGGAAGATCA GCTCTGACTT GGACGGGCAT CCTGTCCCGA AACAAGCCTT CACCGATGTG 
GCCACTGGCT CCCTGGGCCA GGGCCTGGGA GCTGCTTGCG GGATGGCATA CACAGGCAAA 
TACTTTGACA AAGCCAGCTA CCGAGTCTAT TGCATGCTGG GAGACGGGGA GGTCTCCGAG 
GGCTCCGTCT GGGAGGCCAT GGCCTTTGCT GGAATTTACA AGCTGGACAA CCTCGTTGCC 
ATTTTTGACA TCAACCGCCT GGGCCAGAGC GACCCAGCCC CGCTGCAGCA CCAGGTGGAC 
ATCTACCAGA AGCGCTGTGA GGCCTTTGGC TGGCACACCA TCATCGTGGA CGGACACAGC 
GTGGAGGAGC TGTGCAAGGC CTTTGGTCAG GCCAAGCACC AACCAACAGC CATCATTGCC 
AAGACCTTCA AGGGCCGAGG GATCACAGGG ATTGAAGACA AGGAGGCGTG GCACGGGAAG 
CCCCTCCCCA AAAACATGGC CGAGCAGATT ATCCAGGAGA TTTACAGCCA GGTTCAGAGC 
AAAAAGAAGA TCCTGGCCAC GCCCCCTCAG GAGGATGCCC CATCCGTGGA CATTGCTAAC 
ATCCGAATGC CTACGCCACC CAGCTACAAA GTGGGGGACA AGATAGCCAC CCGGAAGGCC 
TATGGACTGG CCCTCGCTAA GCTGGGCCAC GCCAGTGACC GTATCATTGC CCTGGATGGA 
GACACCAAGA ATTCCACCTT CTCGGAGCTC TTCAAAAAGG AGCACCCAGA CCGGTTCATT 
GAGTGCTACA TTGCCGAGCA AAACATGGTG AGCATTGCCG TGGGCTGTGC CACACGTGAC 
CGGACAGTGC CCTTCTGCAG TACTTTCGCG GCCTTCTTCA CACGGGCCTT CGACCAGATT 
CGCATGGCCG CCATCTCTGA GAGCAACATC AACCTCTGTG GCTCCCACTG TGGTGTGTCC 
ATTGGGGAAG ACGGGCCCTC TCAGATGGCC CTCGAAGACC TGGCCATGTT CCGGTCAGTC 
CCCATGTCCA CCGTCTTTTA CCCAAGCGAT GGAGTTGCAA CAGAGAAGGC AGTGGAGTTA 
GCAGCCAACA CAAAGGGCAT TTGCTTCATC CGGACCAGCC GCCCAGAGAA TGCCATTATT 
TATAGCAACA ATGAGGATTT CCAGGTCGGC CAAGCCAAGG TGGTCCTGAA GAGCAAGGAT 
GACCAAGTGA CAGTGATCGG GGCTGGTGTA ACTCTGCATG AGGCCTTGGC TGCTGCAGAG 
AGTCTAAAGA AAGATAAGAT CAGCATCCGG GTGCTGGATC CCTTCACTAT CAAGCCCCTG 
GACAGGAAAC TCATCCTAGA CTCTGCCCGA GCAACCAAAG GCAGGATCCT CACCGTGGAG 
GACCACTACT ACGAAGGTGG CATAGGAGAG GCAGTGTCTG CTGCCGTAGT GGGTGAACCT 
GGAGTGACGG TCACTCGCCT GGCTGTCAGC CAAGTACCAC GAAGTGGCAA GCCAGCTGAG 
CTACTGAAGA TGTTCGGTAT TGACAAGGAC GCCATTGTGC AAGCTGTGAA AGGCCTTGTC 
ACCAAGGGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of transketolase as a target of PARIS in substantia nigra.
Biochemical and biophysical research communications493(2)1050-1056(2017 11)
Kim H,Kang H,Lee Y,Park CH,Jo A,Khang R,Shin JH

Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to Mouse Embryo Mesoderm Development.
Developmental cell40(4)331-341.e4(2017 02)
Bulusu V,Prior N,Snaebjornsson MT,Kuehne A,Sonnen KF,Kress J,Stein F,Schultz C,Sauer U,Aulehla A

Requirement for the Mitochondrial Pyruvate Carrier in Mammalian Development Revealed by a Hypomorphic Allelic Series.
Molecular and cellular biology36(15)2089-104(2016 Aug)
Bowman CE,Zhao L,Hartung T,Wolfgang MJ

Comparative proteomic profiling reveals aberrant cell proliferation in the brain of embryonic Ts1Cje, a mouse model of Down syndrome.
Neuroscience2811-15(2014 Dec)
Ishihara K,Kanai S,Sago H,Yamakawa K,Akiba S

Decreased transketolase activity contributes to impaired hippocampal neurogenesis induced by thiamine deficiency.
Journal of neurochemistry111(2)537-46(2009 Oct)
Zhao Y,Pan X,Zhao J,Wang Y,Peng Y,Zhong C

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Tkt cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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