Atp6v0a2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Atp6v0a2
Entrez Gene ID	21871
Full Name	ATPase, H+ transporting, lysosomal V0 subunit A2
Synonyms	8430408C20Rik,AI385560,ATP6a2,AW489264,Atp6n1d,Atp6n2,C76904,ISF,J6B7,SHIF,Stv1,TJ6M,TJ6s,Tj6,V-ATPase 116 kDa,V-ATPase a2
General protein information	Preferred Names ATPase, H+ transporting, lysosomal V0 subunit A2 Names V-type proton ATPase 116 kDa subunit a isoform 2 ATPase, H+ transporting, lysosomal (vacuolar proton pump) non-catalytic accessory protein 2 (38kD) ATPase, H+ transporting, lysosomal V0 subunit a T-cell expressing clone j6 V-type proton ATPase 116 kDa subunit a immune suppressor factor J6B7 vacuolar proton translocating ATPase 116 kDa subunit a
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 F
Summary	This gene encodes a subunit of vacuolar ATPase, a multimeric enzyme that localizes to intracellular vesicles and to the plasma membrane of specialized cells. The encoded protein is a component of the V(0) domain, which functions in proton translocation across membranes. Function of this gene is important in fetal-specific immune suppression during pregnancy. [provided by RefSeq, May 2013].

mRNA and Protein(s)

mRNA	Protein	Name
NM_011596.5	NP_035726.2	V-type proton ATPase 116 kDa subunit a isoform 2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ATP6V0A2	XP_543370.2
Bos taurus (cattle)	ATP6V0A2	NP_788810.1
Rattus norvegicus (Norway rat)	Atp6v0a2	NP_446227.2
Pan troglodytes (chimpanzee)	ATP6V0A2	XP_003314058.1
Homo sapiens (human)	ATP6V0A2	NP_036595.2
Mus musculus (house mouse)	Atp6v0a2	NP_035726.2
Gallus gallus (chicken)	ATP6V0A2	NP_990054.1
Danio rerio (zebrafish)	atp6v0a2a	XP_708125.5
Xenopus tropicalis (tropical clawed frog)	atp6v0a2	NP_001016223.1
Danio rerio (zebrafish)	atp6v0a2b	NP_001116219.1

Gene Ontology
Bibliography

Related articles in PubMed

Mammary epithelium-specific inactivation of V-ATPase reduces stiffness of extracellular matrix and enhances metastasis of breast cancer.
Katara GK, Kulshrestha A, Mao L, Wang X, Sahoo M, Ibrahim S, Pamarthy S, Suzue K, Shekhawat GS, Gilman-Sachs A, Beaman KD
Molecular oncology(2017 Nov)

The V-ATPase a2 isoform controls mammary gland development through Notch and TGF-? signaling.
Pamarthy S, Mao L, Katara GK, Fleetwood S, Kulshreshta A, Gilman-Sachs A, Beaman KD
Cell death & disease7(11)e2443(2016 Nov)

A novel secreted form of immune suppressor factor with high homology to vacuolar ATPases identified by a forward genetic approach of functional screening based on cell proliferation.
Tulin EE, Onoda N, Maeda M, Hasegawa M, Nosaka T, Nomura H, Asano S, Kitamura T
The Journal of biological chemistry276(29)27519-26(2001 Jul)

Three subunit a isoforms of mouse vacuolar H(+)-ATPase. Preferential expression of the a3 isoform during osteoclast differentiation.
Toyomura T, Oka T, Yamaguchi C, Wada Y, Futai M
The Journal of biological chemistry275(12)8760-5(2000 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

a2V deficiency disrupts the endolysosomal route in Notch and TGF signaling, thereby impairing mammary gland development.
Title: The V-ATPase a2 isoform controls mammary gland development through Notch and TGF-? signaling.

The data provide novel evidence showing that a2V can regulate the apoptotic pathways, an essential testicular feature, and is necessary for efficient spermatogenesis.
Title: Male fertility and apoptosis in normal spermatogenesis are regulated by vacuolar-ATPase isoform a2.

These observations suggest that a2V plays a crucial role in spermatogenesis by regulating testicular immune responses, and its inhibition in males leads to poor pregnancy outcome in females.
Title: Vacuolar-ATPase isoform a2 regulates macrophages and cytokine profile necessary for normal spermatogenesis in testis.

Report Atp6v0a2 up-regulation/inflammatory response during preimplantation period of pregnancy may lead to successful pregnancy outcome.
Title: V-ATPase upregulation during early pregnancy: a possible link to establishment of an inflammatory response during preimplantation period of pregnancy.

Deceased expression of Atp6v0a2 in the various immune cell populations of the spleen and blood suggests that the maternal environment is not supportive to fetus and leads to poor pregnancy outcome in the abortion-prone mating model
Title: Abortion-prone mating influences alteration of systemic a2 vacuolar ATPase expression in spleen and blood immune cells.

The following Atp6v0a2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Atp6v0a2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu13682	NM_011596.5 Latest version!	Mus musculus ATPase, H+ transporting, lysosomal V0 subunit A2 (Atp6v0a2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$503.30 $719.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Atp6v0a2 ( NM_011596.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035726.2 Mus musculus ATPase, H+ transporting, lysosomal V0 subunit A2 (Atp6v0a2), mRNA.

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CloneID	OMu13682
Clone ID Related Accession (Same CDS sequence)	NM_011596.5
Accession Version	NM_011596.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2571bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Mus musculus(house mouse)
Product	V-type proton ATPase 116 kDa subunit a isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CJ076040.1, AF218252.1, AK017467.1, AK032909.1, AK160318.1 and BP764071.1. On May 2, 2013 this sequence version replaced NM_011596.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC112905.1, SRR1660815.214226.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGGCTCTC TCTTCCGCAG CGAGAGCATG TGCCTGGCGC AGCTCTTCCT GCAGTCGGGC 
ACGGCCTACG AGTGTCTGAG CGCGCTGGGC GAGAAGGGCC TGGTGCAGTT CCGAGACCTC 
AATCAAAATG TAAGTTCTTT TCAAAGAAAA TTCGTCGGTG AGGTAAAGAG GTGTGAAGAG 
CTCGAACGAA TACTGGTGTA CCTGGTGCAG GAAATCACCA GAGCTGATAT TCCCCTGCCT 
GAAGGAGAGG CCAGTCCTCC CGCACCACCT CTTAAACATG TCCTAGAAAT GCAGGAACAG 
CTGCAGAAGC TGGAGGTGGA GCTCAGAGAA GTCACCAAGA ACAAGGAGAA GCTGAGGAAG 
AACCTGTTGG AGCTGGTGGA GTACACCCAC ATGCTGAGGG TCACGAAGAC CTTCCTCAAG 
CGGAATGTGG AGTTTGAGCC CACGTATGAA GAGTTTCCCG CCTTAGAGAA CGACTCTCTG 
TTGGACTACA GCTGCATGCA GCGGCTGGGC GCCAAGCTGG GATTCGTTTC TGGCCTGATT 
CAGCAAGGCA GAGTTGAAGC ATTTGAAAGG ATGCTGTGGC GGGCCTGCAA AGGGTACACC 
ATTGTGACCT ATGCAGAGCT GGACGAGTGC CTGGAGGACC CTGAAACGGG TGAAGTCATA 
AAGTGGTACG TGTTCCTGAT TTCCTTTTGG GGAGAGCAGA TTGGCCACAA GGTTAAGAAG 
ATATGTGACT GTTACCACTG CCACATCTAC CCGTATCCAA ACACGGCTGA GGAGCGCAGG 
GAGATTCAGG AGGGGCTCAA TACTCGGATC CAAGACCTTT ACACGGTGCT GCACAAAACG 
GAGGACTATC TGCGGCAAGT GCTCTGCAAA GCTGCCGAGT CCGTGTGCAG CCGTGTGGTC 
CAGGTGAGGA AGATGAAGGC CATCTACCAC ATGCTCAACA TGTGCAGCTT TGACGTCACC 
AACAAGTGCC TCATAGCTGA GGTCTGGTGC CCCGAGGTGG ACCTGCCAGG CTTGCGCAGA 
GCACTGGAGG AAGGCTCGAG AGAGAGCGGA GCTACAATCC CCTCGTTCAT GAACACAATC 
CCTACGAAAG AAACACCCCC CACTCTGATC CGCACCAACA AATTCACTGA AGGTTTCCAG 
AACATCGTGG ATGCCTATGG AGTCGGGAGC TACAGAGAAG TGAACCCAGC TCTCTTTACC 
ATCATCACCT TCCCGTTCCT GTTTGCTGTG ATGTTTGGCG ACTTTGGGCA CGGCTTTGTC 
ATGTTCCTGT TTGCCCTCTT ACTGGTGTTA AATGAGAATC ACCCCAGACT AAGCCAGTCA 
CAGGAGATCC TTAGGATGTT CTTTGACGGC CGGTATATCC TGCTGCTGAT GGGGCTGTTC 
TCTGTGTACA CCGGCCTCAT CTACAACGAC TGCTTCTCCA AGTCTGTGAA CCTCTTTGGT 
TCTGGGTGGA ACGTATCTGC CATGTACAGC TCCAGCCACT CTCCAGAGGA GCAGAGGAAG 
ATGGTACTTT GGAATGACAG CACCATCAGG CACAGCAGGA CTTTGCAGCT GGACCCGAAT 
ATCCCTGGAG TTTTCCGAGG CCCCTACCCT TTTGGCATTG ATCCTATTTG GAACCTGGCC 
ACAAACCGCC TCACGTTCCT CAACTCCTTC AAGATGAAAA TGTCTGTGAT TTTAGGAATT 
TTTCACATGA CTTTTGGTGT TGTTCTGGGA ATATTTAACC ACTTGCACTT TAGGAAGAAG 
TTCAACGTCT ACCTGGTCTC GGTGCCTGAG ATCCTGTTCA TGCTCTGCAT CTTTGGGTAC 
CTGATTTTCA TGATCATTTA CAAGTGGCTG GCATACTCGG CAGAGACCTC GAGAGAGGCC 
CCGAGCATCC TGATCGAGTT CATTAACATG TTCCTGTTCC CAACCAGCAA GACACACGGG 
CTCTACCCAG GGCAGGCACA CGTCCAGAGA GTGTTGGTGG CTCTCACGGT GCTGGCTGTC 
CCCGTGCTCT TCTTAGGAAA GCCGCTTTTT CTGCTGTGGC TGCACAACGG GCGCAATTGC 
TTTGGCATGA GCCGGAGCGG TTACACACTC GTGAGGAAGG ACAGCGAGGA AGAGGTGTCT 
CTTCTGGGCA ACCAGGACAT AGAAGAGGGC AACAGCCGCA TGGAAGAAGG CTGCCGAGAA 
GTGACGTGTG AGGAGTTTAA CTTCGGGGAG ATCCTGATGA CGCAGGCCAT CCACTCCATT 
GAGTACTGCC TCGGCTGCAT CTCCAACACC GCCTCCTACC TGAGGCTCTG GGCACTCAGC 
CTGGCCCATG CACAGCTCTC TGATGTGCTG TGGGCCATGC TGATGCGCGT GGGGCTGCGT 
GTGGACACCA CCTATGGGGT CTTGCTGCTG CTGCCTGTCA TGGCTTTCTT TGCAGTTTTG 
ACCATTTTTA TCCTTCTGGT CATGGAGGGT CTTTCTGCAT TCCTCCACGC CATAAGGCTT 
CACTGGGTAG AATTTCAGAA CAAATTCTAC GTTGGTGCAG GCACCAAGTT TGTTCCCTTC 
TCCTTCAGTC TGCTCTCCTC CAAGTTCAGT AATGATGACA GCATAGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035726.2
CDS	123..2693
Translation	MGSLFRSESMCLAQLFLQSGTAYECLSALGEKGLVQFRDLNQNVSSFQRKFVGEVKRCEELERILVYLVQEITRADIPLPEGEASPPAPPLKHVLEMQEQLQKLEVELREVTKNKEKLRKNLLELVEYTHMLRVTKTFLKRNVEFEPTYEEFPALENDSLLDYSCMQRLGAKLGFVSGLIQQGRVEAFERMLWRACKGYTIVTYAELDECLEDPETGEVIKWYVFLISFWGEQIGHKVKKICDCYHCHIYPYPNTAEERREIQEGLNTRIQDLYTVLHKTEDYLRQVLCKAAESVCSRVVQVRKMKAIYHMLNMCSFDVTNKCLIAEVWCPEVDLPGLRRALEEGSRESGATIPSFMNTIPTKETPPTLIRTNKFTEGFQNIVDAYGVGSYREVNPALFTIITFPFLFAVMFGDFGHGFVMFLFALLLVLNENHPRLSQSQEILRMFFDGRYILLLMGLFSVYTGLIYNDCFSKSVNLFGSGWNVSAMYSSSHSPEEQRKMVLWNDSTIRHSRTLQLDPNIPGVFRGPYPFGIDPIWNLATNRLTFLNSFKMKMSVILGIFHMTFGVVLGIFNHLHFRKKFNVYLVSVPEILFMLCIFGYLIFMIIYKWLAYSAETSREAPSILIEFINMFLFPTSKTHGLYPGQAHVQRVLVALTVLAVPVLFLGKPLFLLWLHNGRNCFGMSRSGYTLVRKDSEEEVSLLGNQDIEEGNSRMEEGCREVTCEEFNFGEILMTQAIHSIEYCLGCISNTASYLRLWALSLAHAQLSDVLWAMLMRVGLRVDTTYGVLLLLPVMAFFAVLTIFILLVMEGLSAFLHAIRLHWVEFQNKFYVGAGTKFVPFSFSLLSSKFSNDDSIA

Target ORF information:

RefSeq Version	NM_011596.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus ATPase, H+ transporting, lysosomal V0 subunit A2 (Atp6v0a2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011596.5

ORF Insert Sequence:

ATGGGCTCTC TCTTCCGCAG CGAGAGCATG TGCCTGGCGC AGCTCTTCCT GCAGTCGGGC 
ACGGCCTACG AGTGTCTGAG CGCGCTGGGC GAGAAGGGCC TGGTGCAGTT CCGAGACCTC 
AATCAAAATG TAAGTTCTTT TCAAAGAAAA TTCGTCGGTG AGGTAAAGAG GTGTGAAGAG 
CTCGAACGAA TACTGGTGTA CCTGGTGCAG GAAATCACCA GAGCTGATAT TCCCCTGCCT 
GAAGGAGAGG CCAGTCCTCC CGCACCACCT CTTAAACATG TCCTAGAAAT GCAGGAACAG 
CTGCAGAAGC TGGAGGTGGA GCTCAGAGAA GTCACCAAGA ACAAGGAGAA GCTGAGGAAG 
AACCTGTTGG AGCTGGTGGA GTACACCCAC ATGCTGAGGG TCACGAAGAC CTTCCTCAAG 
CGGAATGTGG AGTTTGAGCC CACGTATGAA GAGTTTCCCG CCTTAGAGAA CGACTCTCTG 
TTGGACTACA GCTGCATGCA GCGGCTGGGC GCCAAGCTGG GATTCGTTTC TGGCCTGATT 
CAGCAAGGCA GAGTTGAAGC ATTTGAAAGG ATGCTGTGGC GGGCCTGCAA AGGGTACACC 
ATTGTGACCT ATGCAGAGCT GGACGAGTGC CTGGAGGACC CTGAAACGGG TGAAGTCATA 
AAGTGGTACG TGTTCCTGAT TTCCTTTTGG GGAGAGCAGA TTGGCCACAA GGTTAAGAAG 
ATATGTGACT GTTACCACTG CCACATCTAC CCGTATCCAA ACACGGCTGA GGAGCGCAGG 
GAGATTCAGG AGGGGCTCAA TACTCGGATC CAAGACCTTT ACACGGTGCT GCACAAAACG 
GAGGACTATC TGCGGCAAGT GCTCTGCAAA GCTGCCGAGT CCGTGTGCAG CCGTGTGGTC 
CAGGTGAGGA AGATGAAGGC CATCTACCAC ATGCTCAACA TGTGCAGCTT TGACGTCACC 
AACAAGTGCC TCATAGCTGA GGTCTGGTGC CCCGAGGTGG ACCTGCCAGG CTTGCGCAGA 
GCACTGGAGG AAGGCTCGAG AGAGAGCGGA GCTACAATCC CCTCGTTCAT GAACACAATC 
CCTACGAAAG AAACACCCCC CACTCTGATC CGCACCAACA AATTCACTGA AGGTTTCCAG 
AACATCGTGG ATGCCTATGG AGTCGGGAGC TACAGAGAAG TGAACCCAGC TCTCTTTACC 
ATCATCACCT TCCCGTTCCT GTTTGCTGTG ATGTTTGGCG ACTTTGGGCA CGGCTTTGTC 
ATGTTCCTGT TTGCCCTCTT ACTGGTGTTA AATGAGAATC ACCCCAGACT AAGCCAGTCA 
CAGGAGATCC TTAGGATGTT CTTTGACGGC CGGTATATCC TGCTGCTGAT GGGGCTGTTC 
TCTGTGTACA CCGGCCTCAT CTACAACGAC TGCTTCTCCA AGTCTGTGAA CCTCTTTGGT 
TCTGGGTGGA ACGTATCTGC CATGTACAGC TCCAGCCACT CTCCAGAGGA GCAGAGGAAG 
ATGGTACTTT GGAATGACAG CACCATCAGG CACAGCAGGA CTTTGCAGCT GGACCCGAAT 
ATCCCTGGAG TTTTCCGAGG CCCCTACCCT TTTGGCATTG ATCCTATTTG GAACCTGGCC 
ACAAACCGCC TCACGTTCCT CAACTCCTTC AAGATGAAAA TGTCTGTGAT TTTAGGAATT 
TTTCACATGA CTTTTGGTGT TGTTCTGGGA ATATTTAACC ACTTGCACTT TAGGAAGAAG 
TTCAACGTCT ACCTGGTCTC GGTGCCTGAG ATCCTGTTCA TGCTCTGCAT CTTTGGGTAC 
CTGATTTTCA TGATCATTTA CAAGTGGCTG GCATACTCGG CAGAGACCTC GAGAGAGGCC 
CCGAGCATCC TGATCGAGTT CATTAACATG TTCCTGTTCC CAACCAGCAA GACACACGGG 
CTCTACCCAG GGCAGGCACA CGTCCAGAGA GTGTTGGTGG CTCTCACGGT GCTGGCTGTC 
CCCGTGCTCT TCTTAGGAAA GCCGCTTTTT CTGCTGTGGC TGCACAACGG GCGCAATTGC 
TTTGGCATGA GCCGGAGCGG TTACACACTC GTGAGGAAGG ACAGCGAGGA AGAGGTGTCT 
CTTCTGGGCA ACCAGGACAT AGAAGAGGGC AACAGCCGCA TGGAAGAAGG CTGCCGAGAA 
GTGACGTGTG AGGAGTTTAA CTTCGGGGAG ATCCTGATGA CGCAGGCCAT CCACTCCATT 
GAGTACTGCC TCGGCTGCAT CTCCAACACC GCCTCCTACC TGAGGCTCTG GGCACTCAGC 
CTGGCCCATG CACAGCTCTC TGATGTGCTG TGGGCCATGC TGATGCGCGT GGGGCTGCGT 
GTGGACACCA CCTATGGGGT CTTGCTGCTG CTGCCTGTCA TGGCTTTCTT TGCAGTTTTG 
ACCATTTTTA TCCTTCTGGT CATGGAGGGT CTTTCTGCAT TCCTCCACGC CATAAGGCTT 
CACTGGGTAG AATTTCAGAA CAAATTCTAC GTTGGTGCAG GCACCAAGTT TGTTCCCTTC 
TCCTTCAGTC TGCTCTCCTC CAAGTTCAGT AATGATGACA GCATAGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mammary epithelium-specific inactivation of V-ATPase reduces stiffness of extracellular matrix and enhances metastasis of breast cancer.
Molecular oncology(2017 Nov)
Katara GK,Kulshrestha A,Mao L,Wang X,Sahoo M,Ibrahim S,Pamarthy S,Suzue K,Shekhawat GS,Gilman-Sachs A,Beaman KD

The V-ATPase a2 isoform controls mammary gland development through Notch and TGF-? signaling.
Cell death & disease7(11)e2443(2016 Nov)
Pamarthy S,Mao L,Katara GK,Fleetwood S,Kulshreshta A,Gilman-Sachs A,Beaman KD

A novel secreted form of immune suppressor factor with high homology to vacuolar ATPases identified by a forward genetic approach of functional screening based on cell proliferation.
The Journal of biological chemistry276(29)27519-26(2001 Jul)
Tulin EE,Onoda N,Maeda M,Hasegawa M,Nosaka T,Nomura H,Asano S,Kitamura T

Three subunit a isoforms of mouse vacuolar H(+)-ATPase. Preferential expression of the a3 isoform during osteoclast differentiation.
The Journal of biological chemistry275(12)8760-5(2000 Mar)
Toyomura T,Oka T,Yamaguchi C,Wada Y,Futai M

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Atp6v0a2 cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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