Timeless gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
XM_006513583.3	XP_006513646.1	protein timeless homolog isoform X5
NM_001164081.1	NP_001157553.1	protein timeless homolog isoform 2
XM_006513581.3	XP_006513644.1	protein timeless homolog isoform X2
XM_006513580.2	XP_006513643.1	protein timeless homolog isoform X1
XM_006513582.2	XP_006513645.1	protein timeless homolog isoform X3
XM_030245049.1	XP_030100909.1	protein timeless homolog isoform X4
NM_001164080.1	NP_001157552.1	protein timeless homolog isoform 3
NM_001136082.2	NP_001129554.1	protein timeless homolog isoform 1
XM_006513581.4	XP_006513644.1	protein timeless homolog isoform X2
XM_006513580.1	XP_006513643.1	protein timeless homolog isoform X1
XM_006513582.1	XP_006513645.1	protein timeless homolog isoform X3
XM_030245050.1	XP_030100910.1	protein timeless homolog isoform X6
NM_011589.2	NP_035719.1	protein timeless homolog isoform 2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu85428	XM_030245050.1 Latest version!	Mus musculus timeless circadian clock 1 (Timeless), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu44454	XM_006513580.2 Latest version!	Mus musculus timeless circadian clock 1 (Timeless), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu44455	XM_006513581.4 Latest version!	Mus musculus timeless circadian clock 1 (Timeless), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu44456	XM_006513582.2 Latest version!	Mus musculus timeless circadian clock 1 (Timeless), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu85427	XM_030245049.1 Latest version!	Mus musculus timeless circadian clock 1 (Timeless), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu16520	NM_011589.2 Latest version!	Mus musculus timeless circadian clock 1 (Timeless), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu16520	NM_001164081.1 Latest version!	Mus musculus timeless circadian clock 1 (Timeless), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu16551	NM_001136082.2 Latest version!	Mus musculus timeless circadian clock 1 (Timeless), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$706.30 $1009.00
OMu16574	NM_001164080.1 Latest version!	Mus musculus timeless circadian clock 1 (Timeless), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$587.30 $839.00
OMu16520	XM_006513583.3 Latest version!	Mus musculus timeless circadian clock 1 (Timeless), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
View Old Accession Versions >>
OMu44455	XM_006513581.3	Mus musculus timeless circadian clock 1 (Timeless), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OMu44454	XM_006513580.1	Mus musculus timeless circadian clock 1 (Timeless), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OMu44456	XM_006513582.1	Mus musculus timeless circadian clock 1 (Timeless), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
Hide Old Accession Versions >>

Timeless ( XM_030245050.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030100910.1 Mus musculus timeless circadian clock 1 (Timeless), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMu85428
Clone ID Related Accession (Same CDS sequence)	XM_030245050.1
Accession Version	XM_030245050.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2523bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein timeless homolog isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGCGG TCAAGGATCA TCTGCTTCGG GAGAGAGCGC AGCAGCATGA CGAGACTTAC 
TACATGTGGG CAATGGCTTT CTTCATGGCC TTCAACCGAG CTGCCACCTT CCGCCCCGGC 
CTTGTTTCTG AGACCCTCAG TATCCGTACC TTTCACTTTG TGGAGCAGAA CCTCACCAAC 
TACTACGAGA TGATGCTGAC AGACCGCAAG GAGGCCGCCT CCTGGGCGCG CAGGATGCAC 
CTGGCCCTGA AGGCCTACCA GGAGCTGCTG GCCACGGTGA ACGAGATGGA CATGTGCCCA 
GATGAGGCTG TTAGGGAGAG CAGTCGTATC ATCAAAAACA ACATTTTCTA TATGATGGAG 
TACCGAGAAC TATTCCTGGC GCTCTTTCGA AAGTTTGATG AGAGATACCA TCCACGCTCA 
TTCCTTCGAG ACCTGGTGGA AACCACCCAC CTCTTCCTCA AAATGTTGGA GCGCTTTTGC 
CGGAGCCGCG GGAACCTGAT GGTGCAGAAC AAAAGAAAAA AGAGGAAAAA GAAAAAGAAG 
GTTCAGGACC AGGGTGTTGC TTTCTCACAA AGCCCCGGGG AGCTGGAGGC CATGTGGCCA 
GCCCTGGCAG AGCAGCTGCT GCAGTGTGCC CAGGACCCTG AGCTCAGTGT GGACCCCGTC 
GTTCCCTTTG ATGCGGCCTC AGAGGTGCCA GTGGAGGAGC AGCGGGTAGA AGCCATGGTG 
AGGATCCAAG ACTGCCTTAC GGCTGGCCAG GCCCCGCAAG CCCTGGCCCT CCTGCGGTCT 
GCCCGGGAAG TGTGGCCTGA AGGAAATGCG TTTGGCTCTC CAGTCATTTC CCCAGGGGAA 
GAAATGCAGT TGCTGAAACA AATCCTCTCC ACGCCCCTTC CCCGGCAGCA GGAGCCAGAA 
GAAGGAGATG CAGAGGAGGA AGAGGAAGAG GAGGAGGAAG AGGAGTTACA GGTGGTCCAG 
GTGTCAGAGA AGGAGTTTAA CTTTCTGGAA TACCTGAAAC GCTTCGCATC CTCAACCATC 
GTGCGGGCCT ACGTGCTTCT CCTGCGGAGC TACAGGCAGA ACAGTGCTCA CACCAACCAC 
TGCATCGCCA AGATGCTGCA CCGGCTGGCC CATGGCCTGG GGATGGAAGC CCTGCTTTTC 
CAGCTGTCCC TGTTCTGCCT CTTCAATCGG CTGCTTAGTG ACCCAGCTGC TGCGGCCTAC 
AAAGAGCTAG TGACTTTTGC CAAATACATC ATTGGCAAGT TCTTTGCGTT GGCTGCCGTG 
AACCAGAAAG CGTTTGTGGA GCTGCTGTTC TGGAAGAACA CCGCAGTGGT TCGGGAAATG 
ACCCAGGGAT ATGGCTCCCT CGACAGTGGG TCTTCCAGCC ACAGAGCTCC TCTGTGGAGC 
CCTGAGGAAG AGGCCCAGCT TCAGGAACTA TACCTCGCCC ACAAGGATGT GGAAGGTCAA 
GATGTAGTGG AAACCATATT GGCGCACCTG AAAGTCGTTC CTCGAACACG CAAGCAGGTC 
ATCCACCACC TGGTCCGGAT GGGCCTGGCC GACAGCGTCA AGGAGTTCCA GAAGAGGAAA 
GGGACCCAGA TTGTCTTGTG GACGGAGGAC CAGGAGCTGG AGTTACAGCG GCTCTTTGAG 
GAGTTCCGGG ACTCTGATGA TGTTCTTGGT CAAATCATGA AGAATATCAC AGCCAAACGT 
TCACGGGCTC GAGTAGTGGA CAAACTGTTG GCCCTGGGGT TGGTGTCTGA GCGGAGGCAG 
CTATACAAGA AACGGAGAAA GAAGCTGGCG CCTTCTTGCA TGCAGAATGG AGAAAAGTCC 
CCGAGAGACC CCTGGCAGGA AGATCCGGAA GAGGAAGACG AACACTTGCC AGAGGACGAA 
AGTGAAGATG AGGAGAGTGA GGAAGGCTTG CCATCAGGAC AGGGTCAGGG CAGCTCATCT 
CTCTCTGCTG AAAACCTCGG TGAGAGCCTT CGTCAGGAAG GCCTCTCTGC TCCCCTCCTG 
TGGCTCCAGA GCTCCCTGAT CCGAGCAGCA AATGACCGAG AAGAGGATGG CTGCTCCCAG 
GCAATCCCTC TGGTGCCTCT GACAGAGGAA AATGAGGAAG CAATGGAGAA CGAACAGTTT 
CAGCATCTGC TACGCAAGCT AGGGATCCGG CCGCCCAGCT CAGGGCAGGA AACCTTCTGG 
AGAATTCCAG CCAAACTGAG CTCCACCCAG CTTCGGAGGG TGGCTGCTTC TTTGAGTCAG 
CAAGAAAACG AGGAGGAAAG GGAAGAGGAG CCAGAGCCAG GAGTCCCCGG AGAGCAGGGT 
CCCAGTGAGG AGCACCGGAC AGAAGCCCTG AGAGCCCTTC TGTCAGCCCG TAAGAGGAAA 
GCAGGCCTGG GGCCTACAGA AGAGGAGGCC ACTGGGGAGG AAGAATGGAA CTCAGCGCCC 
AAGAAGCGGC AACTGCTGGA CAGCGACGAA GAGGAAGATG ATGAGGGGAG GAGGCAAGCA 
GTGTCGGGAA CGCCAAGAGT CCACAGGAAG AAACGGTTTC AGATTGAGGA TGAGGATGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030100910.1
CDS	1346..3868
Translation	MGAVKDHLLRERAQQHDETYYMWAMAFFMAFNRAATFRPGLVSETLSIRTFHFVEQNLTNYYEMMLTDRKEAASWARRMHLALKAYQELLATVNEMDMCPDEAVRESSRIIKNNIFYMMEYRELFLALFRKFDERYHPRSFLRDLVETTHLFLKMLERFCRSRGNLMVQNKRKKRKKKKKVQDQGVAFSQSPGELEAMWPALAEQLLQCAQDPELSVDPVVPFDAASEVPVEEQRVEAMVRIQDCLTAGQAPQALALLRSAREVWPEGNAFGSPVISPGEEMQLLKQILSTPLPRQQEPEEGDAEEEEEEEEEEELQVVQVSEKEFNFLEYLKRFASSTIVRAYVLLLRSYRQNSAHTNHCIAKMLHRLAHGLGMEALLFQLSLFCLFNRLLSDPAAAAYKELVTFAKYIIGKFFALAAVNQKAFVELLFWKNTAVVREMTQGYGSLDSGSSSHRAPLWSPEEEAQLQELYLAHKDVEGQDVVETILAHLKVVPRTRKQVIHHLVRMGLADSVKEFQKRKGTQIVLWTEDQELELQRLFEEFRDSDDVLGQIMKNITAKRSRARVVDKLLALGLVSERRQLYKKRRKKLAPSCMQNGEKSPRDPWQEDPEEEDEHLPEDESEDEESEEGLPSGQGQGSSSLSAENLGESLRQEGLSAPLLWLQSSLIRAANDREEDGCSQAIPLVPLTEENEEAMENEQFQHLLRKLGIRPPSSGQETFWRIPAKLSSTQLRRVAASLSQQENEEEREEEPEPGVPGEQGPSEEHRTEALRALLSARKRKAGLGPTEEEATGEEEWNSAPKKRQLLDSDEEEDDEGRRQAVSGTPRVHRKKRFQIEDEDD

Target ORF information:

RefSeq Version	XM_030245050.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus timeless circadian clock 1 (Timeless), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030245050.1

ORF Insert Sequence:

ATGGGCGCGG TCAAGGATCA TCTGCTTCGG GAGAGAGCGC AGCAGCATGA CGAGACTTAC 
TACATGTGGG CAATGGCTTT CTTCATGGCC TTCAACCGAG CTGCCACCTT CCGCCCCGGC 
CTTGTTTCTG AGACCCTCAG TATCCGTACC TTTCACTTTG TGGAGCAGAA CCTCACCAAC 
TACTACGAGA TGATGCTGAC AGACCGCAAG GAGGCCGCCT CCTGGGCGCG CAGGATGCAC 
CTGGCCCTGA AGGCCTACCA GGAGCTGCTG GCCACGGTGA ACGAGATGGA CATGTGCCCA 
GATGAGGCTG TTAGGGAGAG CAGTCGTATC ATCAAAAACA ACATTTTCTA TATGATGGAG 
TACCGAGAAC TATTCCTGGC GCTCTTTCGA AAGTTTGATG AGAGATACCA TCCACGCTCA 
TTCCTTCGAG ACCTGGTGGA AACCACCCAC CTCTTCCTCA AAATGTTGGA GCGCTTTTGC 
CGGAGCCGCG GGAACCTGAT GGTGCAGAAC AAAAGAAAAA AGAGGAAAAA GAAAAAGAAG 
GTTCAGGACC AGGGTGTTGC TTTCTCACAA AGCCCCGGGG AGCTGGAGGC CATGTGGCCA 
GCCCTGGCAG AGCAGCTGCT GCAGTGTGCC CAGGACCCTG AGCTCAGTGT GGACCCCGTC 
GTTCCCTTTG ATGCGGCCTC AGAGGTGCCA GTGGAGGAGC AGCGGGTAGA AGCCATGGTG 
AGGATCCAAG ACTGCCTTAC GGCTGGCCAG GCCCCGCAAG CCCTGGCCCT CCTGCGGTCT 
GCCCGGGAAG TGTGGCCTGA AGGAAATGCG TTTGGCTCTC CAGTCATTTC CCCAGGGGAA 
GAAATGCAGT TGCTGAAACA AATCCTCTCC ACGCCCCTTC CCCGGCAGCA GGAGCCAGAA 
GAAGGAGATG CAGAGGAGGA AGAGGAAGAG GAGGAGGAAG AGGAGTTACA GGTGGTCCAG 
GTGTCAGAGA AGGAGTTTAA CTTTCTGGAA TACCTGAAAC GCTTCGCATC CTCAACCATC 
GTGCGGGCCT ACGTGCTTCT CCTGCGGAGC TACAGGCAGA ACAGTGCTCA CACCAACCAC 
TGCATCGCCA AGATGCTGCA CCGGCTGGCC CATGGCCTGG GGATGGAAGC CCTGCTTTTC 
CAGCTGTCCC TGTTCTGCCT CTTCAATCGG CTGCTTAGTG ACCCAGCTGC TGCGGCCTAC 
AAAGAGCTAG TGACTTTTGC CAAATACATC ATTGGCAAGT TCTTTGCGTT GGCTGCCGTG 
AACCAGAAAG CGTTTGTGGA GCTGCTGTTC TGGAAGAACA CCGCAGTGGT TCGGGAAATG 
ACCCAGGGAT ATGGCTCCCT CGACAGTGGG TCTTCCAGCC ACAGAGCTCC TCTGTGGAGC 
CCTGAGGAAG AGGCCCAGCT TCAGGAACTA TACCTCGCCC ACAAGGATGT GGAAGGTCAA 
GATGTAGTGG AAACCATATT GGCGCACCTG AAAGTCGTTC CTCGAACACG CAAGCAGGTC 
ATCCACCACC TGGTCCGGAT GGGCCTGGCC GACAGCGTCA AGGAGTTCCA GAAGAGGAAA 
GGGACCCAGA TTGTCTTGTG GACGGAGGAC CAGGAGCTGG AGTTACAGCG GCTCTTTGAG 
GAGTTCCGGG ACTCTGATGA TGTTCTTGGT CAAATCATGA AGAATATCAC AGCCAAACGT 
TCACGGGCTC GAGTAGTGGA CAAACTGTTG GCCCTGGGGT TGGTGTCTGA GCGGAGGCAG 
CTATACAAGA AACGGAGAAA GAAGCTGGCG CCTTCTTGCA TGCAGAATGG AGAAAAGTCC 
CCGAGAGACC CCTGGCAGGA AGATCCGGAA GAGGAAGACG AACACTTGCC AGAGGACGAA 
AGTGAAGATG AGGAGAGTGA GGAAGGCTTG CCATCAGGAC AGGGTCAGGG CAGCTCATCT 
CTCTCTGCTG AAAACCTCGG TGAGAGCCTT CGTCAGGAAG GCCTCTCTGC TCCCCTCCTG 
TGGCTCCAGA GCTCCCTGAT CCGAGCAGCA AATGACCGAG AAGAGGATGG CTGCTCCCAG 
GCAATCCCTC TGGTGCCTCT GACAGAGGAA AATGAGGAAG CAATGGAGAA CGAACAGTTT 
CAGCATCTGC TACGCAAGCT AGGGATCCGG CCGCCCAGCT CAGGGCAGGA AACCTTCTGG 
AGAATTCCAG CCAAACTGAG CTCCACCCAG CTTCGGAGGG TGGCTGCTTC TTTGAGTCAG 
CAAGAAAACG AGGAGGAAAG GGAAGAGGAG CCAGAGCCAG GAGTCCCCGG AGAGCAGGGT 
CCCAGTGAGG AGCACCGGAC AGAAGCCCTG AGAGCCCTTC TGTCAGCCCG TAAGAGGAAA 
GCAGGCCTGG GGCCTACAGA AGAGGAGGCC ACTGGGGAGG AAGAATGGAA CTCAGCGCCC 
AAGAAGCGGC AACTGCTGGA CAGCGACGAA GAGGAAGATG ATGAGGGGAG GAGGCAAGCA 
GTGTCGGGAA CGCCAAGAGT CCACAGGAAG AAACGGTTTC AGATTGAGGA TGAGGATGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Timeless ( XM_006513580.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006513643.1 Mus musculus timeless circadian clock 1 (Timeless), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu44454
Clone ID Related Accession (Same CDS sequence)	XM_006513580.2 , XM_006513580.1
Accession Version	XM_006513580.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3777bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein timeless homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006513580.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACAGAA GCTACCTCAC CAGAGACCAG GTCTCTGAGA ACGGAAGCTA CCTCACCAGA 
GACCAGGTCT CTGAGAACGG GCCAGAAGAA AAGGCTCCTT CCAGTCCTGT CGTTCATCCC 
GTAGGCCTCA CCCTCTCCGT CCACCATCTC TACTGCCCGC TCTGCTGGTT GGGCCTCTGG 
TGTATGGACT TGTACATGAT GAACTGTGAA CTTCTAGCCA CGTGTAGCGC CCTTGGGTAC 
TTGGAAGGAG GGACTTACCA CAAGGAGCCG GATTGCCTGG AGAGTGTGAA GGATTTGATC 
CGATACCTGA GGCACGAGGA TGAGACCCGA GATGTGCGGC AGCAGCTGGG AGCTGCACAG 
ATCCTGCAGA GCGACCTCCT GCCAATCCTC ACGCAGCATC GCCAGGACAA GCCTCTCTTC 
GATGCCGTGA TCAGGCTGAT GGTAAATTTG ACACAGCCAG CCTTGCTCTG TTTTGGCAGC 
GTGCCTAAGG ACTCCAGTGT ACGGCACCAT TTTCTGCAGG TTCTAACGTA CCTGCAAGCC 
TACAAAGAGG CCTTTGCCAG TGAGAAGGCA TTTGGAGTCC TCAGCGAGAC CTTGTATGAA 
TTGCTACAGC TGGGCTGGGA GGATCGGCAA GAAGAAGACA ACTTGCTGAT CGAGCGGATC 
CTTCTGCTGG TCAGAAATAT TCTCCATGTC CCGGCCAACC TTGAGCAGGA GAAGAGTATC 
GATGATGATG CCAGCATCCA CGACCGTCTC CTTTGGGCAA TTCACCTCAG TGGCATGGAC 
GACTTGCTCC TCTTCCTGTC CAGCTCATCC GCCGAGCAGC AGTGGAGCCT CCATGTGCTG 
GAGATCATCT CCCTCATGTT CCGAGACCAG ACCCCTGAGC AGCTAGCGGG AGTAGGGCAG 
GGACGCTTGG CTCAGGAGCG AAGCACGGAT GTGGCAGAAT TGGAGGTGCT GCGCCAACGG 
GAGATGGCGG AGAAGAGAGC TCGGGCCCTC CAGCGAGGAA ACAGGCACTC TCGATTTGGG 
GGCTCCTACA TTGTCCAGGG GTTGAAATCT ATTGGGGAGA AGGATGTCGT CTTTCACAAA 
GGCCTTCACA ATCTCCAGAA CTACAGCTCA GATCTGGGAA AGCAGCCCAG GAGGGTGCCC 
AAGCGTCGTC AGGCTGCCCA GGAGCTGTCT GTCCATCGCC GCTCTGTCCT GAATGTGAGA 
CTCTTCCTCA GAGACTTCTG CTCTGAGTTC CTGGAGAACT GCTACAACCC GCTCATGGGC 
GCGGTCAAGG ATCATCTGCT TCGGGAGAGA GCGCAGCAGC ATGACGAGAC TTACTACATG 
TGGGCAATGG CTTTCTTCAT GGCCTTCAAC CGAGCTGCCA CCTTCCGCCC CGGCCTTGTT 
TCTGAGACCC TCAGTATCCG TACCTTTCAC TTTGTGGAGC AGAACCTCAC CAACTACTAC 
GAGATGATGC TGACAGACCG CAAGGAGGCC GCCTCCTGGG CGCGCAGGAT GCACCTGGCC 
CTGAAGGCCT ACCAGGAGCT GCTGGCCACG GTGAACGAGA TGGACATGTG CCCAGATGAG 
GCTGTTAGGG AGAGCAGTCG TATCATCAAA AACAACATTT TCTATATGAT GGAGTACCGA 
GAACTATTCC TGGCGCTCTT TCGAAAGTTT GATGAGAGAT ACCATCCACG CTCATTCCTT 
CGAGACCTGG TGGAAACCAC CCACCTCTTC CTCAAAATGT TGGAGCGCTT TTGCCGGAGC 
CGCGGGAACC TGATGGTGCA GAACAAAAGA AAAAAGAGGA AAAAGAAAAA GAAGGTTCAG 
GACCAGGGTG TTGCTTTCTC ACAAAGCCCC GGGGAGCTGG AGGCCATGTG GCCAGCCCTG 
GCAGAGCAGC TGCTGCAGTG TGCCCAGGAC CCTGAGCTCA GTGTGGACCC CGTCGTTCCC 
TTTGATGCGG CCTCAGAGGT GCCAGTGGAG GAGCAGCGGG TAGAAGCCAT GGTGAGGATC 
CAAGACTGCC TTACGGCTGG CCAGGCCCCG CAAGCCCTGG CCCTCCTGCG GTCTGCCCGG 
GAAGTGTGGC CTGAAGGAAA TGCGTTTGGC TCTCCAGTCA TTTCCCCAGG GGAAGAAATG 
CAGTTGCTGA AACAAATCCT CTCCACGCCC CTTCCCCGGC AGCAGGAGCC AGAAGAAGGA 
GATGCAGAGG AGGAAGAGGA AGAGGAGGAG GAAGAGGAGT TACAGGTGGT CCAGGTGTCA 
GAGAAGGAGT TTAACTTTCT GGAATACCTG AAACGCTTCG CATCCTCAAC CATCGTGCGG 
GCCTACGTGC TTCTCCTGCG GAGCTACAGG CAGAACAGTG CTCACACCAA CCACTGCATC 
GCCAAGATGC TGCACCGGCT GGCCCATGGC CTGGGGATGG AAGCCCTGCT TTTCCAGCTG 
TCCCTGTTCT GCCTCTTCAA TCGGCTGCTT AGTGACCCAG CTGCTGCGGC CTACAAAGAG 
CTAGTGACTT TTGCCAAATA CATCATTGGC AAGTTCTTTG CGTTGGCTGC CGTGAACCAG 
AAAGCGTTTG TGGAGCTGCT GTTCTGGAAG AACACCGCAG TGGTTCGGGA AATGACCCAG 
GGATATGGCT CCCTCGACAG TGGGTCTTCC AGCCACAGAG CTCCTCTGTG GAGCCCTGAG 
GAAGAGGCCC AGCTTCAGGA ACTATACCTC GCCCACAAGG ATGTGGAAGG TCAAGATGTA 
GTGGAAACCA TATTGGCGCA CCTGAAAGTC GTTCCTCGAA CACGCAAGCA GGTCATCCAC 
CACCTGGTCC GGATGGGCCT GGCCGACAGC GTCAAGGAGT TCCAGAAGAG GAAAGGGACC 
CAGATTGTCT TGTGGACGGA GGACCAGGAG CTGGAGTTAC AGCGGCTCTT TGAGGAGTTC 
CGGGACTCTG ATGATGTTCT TGGTCAAATC ATGAAGAATA TCACAGCCAA ACGTTCACGG 
GCTCGAGTAG TGGACAAACT GTTGGCCCTG GGGTTGGTGT CTGAGCGGAG GCAGCTATAC 
AAGAAACGGA GAAAGAAGCT GGCGCCTTCT TGCATGCAGA ATGGAGAAAA GTCCCCGAGA 
GACCCCTGGC AGGAAGATCC GGAAGAGGAA GACGAACACT TGCCAGAGGA CGAAAGTGAA 
GATGAGGAGA GTGAGGAAGG CTTGCCATCA GGACAGGGTC AGGGCAGCTC ATCTCTCTCT 
GCTGAAAACC TCGGTGAGAG CCTTCGTCAG GAAGGCCTCT CTGCTCCCCT CCTGTGGCTC 
CAGAGCTCCC TGATCCGAGC AGCAAATGAC CGAGAAGAGG ATGGCTGCTC CCAGGCAATC 
CCTCTGGTGC CTCTGACAGA GGAAAATGAG GAAGCAATGG AGAACGAACA GTTTCAGCAT 
CTGCTACGCA AGCTAGGGAT CCGGCCGCCC AGCTCAGGGC AGGAAACCTT CTGGAGAATT 
CCAGCCAAAC TGAGCTCCAC CCAGCTTCGG AGGGTGGCTG CTTCTTTGAG TCAGCAAGAA 
AACGAGGAGG AAAGGGAAGA GGAGCCAGAG CCAGGAGTCC CCGGAGAGCA GGGTCCCAGT 
GAGGAGCACC GGACAGAAGC CCTGAGAGCC CTTCTGTCAG CCCGTAAGAG GAAAGCAGGC 
CTGGGGCCTA CAGAAGAGGA GGCCACTGGG GAGGAAGAAT GGAACTCAGC GCCCAAGAAG 
CGGCAACTGC TGGACAGCGA CGAAGAGGAA GATGATGAGG GGAGGAGGCA AGCAGTGTCG 
GGAACGCCAA GAGTCCACAG GAAGAAACGG TTTCAGATTG AGGATGAGGA TGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006513643.1
CDS	1605..5381
Translation	MYRSYLTRDQVSENGSYLTRDQVSENGPEEKAPSSPVVHPVGLTLSVHHLYCPLCWLGLWCMDLYMMNCELLATCSALGYLEGGTYHKEPDCLESVKDLIRYLRHEDETRDVRQQLGAAQILQSDLLPILTQHRQDKPLFDAVIRLMVNLTQPALLCFGSVPKDSSVRHHFLQVLTYLQAYKEAFASEKAFGVLSETLYELLQLGWEDRQEEDNLLIERILLLVRNILHVPANLEQEKSIDDDASIHDRLLWAIHLSGMDDLLLFLSSSSAEQQWSLHVLEIISLMFRDQTPEQLAGVGQGRLAQERSTDVAELEVLRQREMAEKRARALQRGNRHSRFGGSYIVQGLKSIGEKDVVFHKGLHNLQNYSSDLGKQPRRVPKRRQAAQELSVHRRSVLNVRLFLRDFCSEFLENCYNPLMGAVKDHLLRERAQQHDETYYMWAMAFFMAFNRAATFRPGLVSETLSIRTFHFVEQNLTNYYEMMLTDRKEAASWARRMHLALKAYQELLATVNEMDMCPDEAVRESSRIIKNNIFYMMEYRELFLALFRKFDERYHPRSFLRDLVETTHLFLKMLERFCRSRGNLMVQNKRKKRKKKKKVQDQGVAFSQSPGELEAMWPALAEQLLQCAQDPELSVDPVVPFDAASEVPVEEQRVEAMVRIQDCLTAGQAPQALALLRSAREVWPEGNAFGSPVISPGEEMQLLKQILSTPLPRQQEPEEGDAEEEEEEEEEEELQVVQVSEKEFNFLEYLKRFASSTIVRAYVLLLRSYRQNSAHTNHCIAKMLHRLAHGLGMEALLFQLSLFCLFNRLLSDPAAAAYKELVTFAKYIIGKFFALAAVNQKAFVELLFWKNTAVVREMTQGYGSLDSGSSSHRAPLWSPEEEAQLQELYLAHKDVEGQDVVETILAHLKVVPRTRKQVIHHLVRMGLADSVKEFQKRKGTQIVLWTEDQELELQRLFEEFRDSDDVLGQIMKNITAKRSRARVVDKLLALGLVSERRQLYKKRRKKLAPSCMQNGEKSPRDPWQEDPEEEDEHLPEDESEDEESEEGLPSGQGQGSSSLSAENLGESLRQEGLSAPLLWLQSSLIRAANDREEDGCSQAIPLVPLTEENEEAMENEQFQHLLRKLGIRPPSSGQETFWRIPAKLSSTQLRRVAASLSQQENEEEREEEPEPGVPGEQGPSEEHRTEALRALLSARKRKAGLGPTEEEATGEEEWNSAPKKRQLLDSDEEEDDEGRRQAVSGTPRVHRKKRFQIEDEDD

Target ORF information:

RefSeq Version	XM_006513580.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus timeless circadian clock 1 (Timeless), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006513580.2

ORF Insert Sequence:

ATGTACAGAA GCTACCTCAC CAGAGACCAG GTCTCTGAGA ACGGAAGCTA CCTCACCAGA 
GACCAGGTCT CTGAGAACGG GCCAGAAGAA AAGGCTCCTT CCAGTCCTGT CGTTCATCCC 
GTAGGCCTCA CCCTCTCCGT CCACCATCTC TACTGCCCGC TCTGCTGGTT GGGCCTCTGG 
TGTATGGACT TGTACATGAT GAACTGTGAA CTTCTAGCCA CGTGTAGCGC CCTTGGGTAC 
TTGGAAGGAG GGACTTACCA CAAGGAGCCG GATTGCCTGG AGAGTGTGAA GGATTTGATC 
CGATACCTGA GGCACGAGGA TGAGACCCGA GATGTGCGGC AGCAGCTGGG AGCTGCACAG 
ATCCTGCAGA GCGACCTCCT GCCAATCCTC ACGCAGCATC GCCAGGACAA GCCTCTCTTC 
GATGCCGTGA TCAGGCTGAT GGTAAATTTG ACACAGCCAG CCTTGCTCTG TTTTGGCAGC 
GTGCCTAAGG ACTCCAGTGT ACGGCACCAT TTTCTGCAGG TTCTAACGTA CCTGCAAGCC 
TACAAAGAGG CCTTTGCCAG TGAGAAGGCA TTTGGAGTCC TCAGCGAGAC CTTGTATGAA 
TTGCTACAGC TGGGCTGGGA GGATCGGCAA GAAGAAGACA ACTTGCTGAT CGAGCGGATC 
CTTCTGCTGG TCAGAAATAT TCTCCATGTC CCGGCCAACC TTGAGCAGGA GAAGAGTATC 
GATGATGATG CCAGCATCCA CGACCGTCTC CTTTGGGCAA TTCACCTCAG TGGCATGGAC 
GACTTGCTCC TCTTCCTGTC CAGCTCATCC GCCGAGCAGC AGTGGAGCCT CCATGTGCTG 
GAGATCATCT CCCTCATGTT CCGAGACCAG ACCCCTGAGC AGCTAGCGGG AGTAGGGCAG 
GGACGCTTGG CTCAGGAGCG AAGCACGGAT GTGGCAGAAT TGGAGGTGCT GCGCCAACGG 
GAGATGGCGG AGAAGAGAGC TCGGGCCCTC CAGCGAGGAA ACAGGCACTC TCGATTTGGG 
GGCTCCTACA TTGTCCAGGG GTTGAAATCT ATTGGGGAGA AGGATGTCGT CTTTCACAAA 
GGCCTTCACA ATCTCCAGAA CTACAGCTCA GATCTGGGAA AGCAGCCCAG GAGGGTGCCC 
AAGCGTCGTC AGGCTGCCCA GGAGCTGTCT GTCCATCGCC GCTCTGTCCT GAATGTGAGA 
CTCTTCCTCA GAGACTTCTG CTCTGAGTTC CTGGAGAACT GCTACAACCC GCTCATGGGC 
GCGGTCAAGG ATCATCTGCT TCGGGAGAGA GCGCAGCAGC ATGACGAGAC TTACTACATG 
TGGGCAATGG CTTTCTTCAT GGCCTTCAAC CGAGCTGCCA CCTTCCGCCC CGGCCTTGTT 
TCTGAGACCC TCAGTATCCG TACCTTTCAC TTTGTGGAGC AGAACCTCAC CAACTACTAC 
GAGATGATGC TGACAGACCG CAAGGAGGCC GCCTCCTGGG CGCGCAGGAT GCACCTGGCC 
CTGAAGGCCT ACCAGGAGCT GCTGGCCACG GTGAACGAGA TGGACATGTG CCCAGATGAG 
GCTGTTAGGG AGAGCAGTCG TATCATCAAA AACAACATTT TCTATATGAT GGAGTACCGA 
GAACTATTCC TGGCGCTCTT TCGAAAGTTT GATGAGAGAT ACCATCCACG CTCATTCCTT 
CGAGACCTGG TGGAAACCAC CCACCTCTTC CTCAAAATGT TGGAGCGCTT TTGCCGGAGC 
CGCGGGAACC TGATGGTGCA GAACAAAAGA AAAAAGAGGA AAAAGAAAAA GAAGGTTCAG 
GACCAGGGTG TTGCTTTCTC ACAAAGCCCC GGGGAGCTGG AGGCCATGTG GCCAGCCCTG 
GCAGAGCAGC TGCTGCAGTG TGCCCAGGAC CCTGAGCTCA GTGTGGACCC CGTCGTTCCC 
TTTGATGCGG CCTCAGAGGT GCCAGTGGAG GAGCAGCGGG TAGAAGCCAT GGTGAGGATC 
CAAGACTGCC TTACGGCTGG CCAGGCCCCG CAAGCCCTGG CCCTCCTGCG GTCTGCCCGG 
GAAGTGTGGC CTGAAGGAAA TGCGTTTGGC TCTCCAGTCA TTTCCCCAGG GGAAGAAATG 
CAGTTGCTGA AACAAATCCT CTCCACGCCC CTTCCCCGGC AGCAGGAGCC AGAAGAAGGA 
GATGCAGAGG AGGAAGAGGA AGAGGAGGAG GAAGAGGAGT TACAGGTGGT CCAGGTGTCA 
GAGAAGGAGT TTAACTTTCT GGAATACCTG AAACGCTTCG CATCCTCAAC CATCGTGCGG 
GCCTACGTGC TTCTCCTGCG GAGCTACAGG CAGAACAGTG CTCACACCAA CCACTGCATC 
GCCAAGATGC TGCACCGGCT GGCCCATGGC CTGGGGATGG AAGCCCTGCT TTTCCAGCTG 
TCCCTGTTCT GCCTCTTCAA TCGGCTGCTT AGTGACCCAG CTGCTGCGGC CTACAAAGAG 
CTAGTGACTT TTGCCAAATA CATCATTGGC AAGTTCTTTG CGTTGGCTGC CGTGAACCAG 
AAAGCGTTTG TGGAGCTGCT GTTCTGGAAG AACACCGCAG TGGTTCGGGA AATGACCCAG 
GGATATGGCT CCCTCGACAG TGGGTCTTCC AGCCACAGAG CTCCTCTGTG GAGCCCTGAG 
GAAGAGGCCC AGCTTCAGGA ACTATACCTC GCCCACAAGG ATGTGGAAGG TCAAGATGTA 
GTGGAAACCA TATTGGCGCA CCTGAAAGTC GTTCCTCGAA CACGCAAGCA GGTCATCCAC 
CACCTGGTCC GGATGGGCCT GGCCGACAGC GTCAAGGAGT TCCAGAAGAG GAAAGGGACC 
CAGATTGTCT TGTGGACGGA GGACCAGGAG CTGGAGTTAC AGCGGCTCTT TGAGGAGTTC 
CGGGACTCTG ATGATGTTCT TGGTCAAATC ATGAAGAATA TCACAGCCAA ACGTTCACGG 
GCTCGAGTAG TGGACAAACT GTTGGCCCTG GGGTTGGTGT CTGAGCGGAG GCAGCTATAC 
AAGAAACGGA GAAAGAAGCT GGCGCCTTCT TGCATGCAGA ATGGAGAAAA GTCCCCGAGA 
GACCCCTGGC AGGAAGATCC GGAAGAGGAA GACGAACACT TGCCAGAGGA CGAAAGTGAA 
GATGAGGAGA GTGAGGAAGG CTTGCCATCA GGACAGGGTC AGGGCAGCTC ATCTCTCTCT 
GCTGAAAACC TCGGTGAGAG CCTTCGTCAG GAAGGCCTCT CTGCTCCCCT CCTGTGGCTC 
CAGAGCTCCC TGATCCGAGC AGCAAATGAC CGAGAAGAGG ATGGCTGCTC CCAGGCAATC 
CCTCTGGTGC CTCTGACAGA GGAAAATGAG GAAGCAATGG AGAACGAACA GTTTCAGCAT 
CTGCTACGCA AGCTAGGGAT CCGGCCGCCC AGCTCAGGGC AGGAAACCTT CTGGAGAATT 
CCAGCCAAAC TGAGCTCCAC CCAGCTTCGG AGGGTGGCTG CTTCTTTGAG TCAGCAAGAA 
AACGAGGAGG AAAGGGAAGA GGAGCCAGAG CCAGGAGTCC CCGGAGAGCA GGGTCCCAGT 
GAGGAGCACC GGACAGAAGC CCTGAGAGCC CTTCTGTCAG CCCGTAAGAG GAAAGCAGGC 
CTGGGGCCTA CAGAAGAGGA GGCCACTGGG GAGGAAGAAT GGAACTCAGC GCCCAAGAAG 
CGGCAACTGC TGGACAGCGA CGAAGAGGAA GATGATGAGG GGAGGAGGCA AGCAGTGTCG 
GGAACGCCAA GAGTCCACAG GAAGAAACGG TTTCAGATTG AGGATGAGGA TGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Timeless ( XM_006513581.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006513644.1 Mus musculus timeless circadian clock 1 (Timeless), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu44455
Clone ID Related Accession (Same CDS sequence)	XM_006513581.3 , XM_006513581.4
Accession Version	XM_006513581.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein timeless homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006513581.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACAGAA GCTACCTCAC CAGAGACCAG GTCTCTGAGA ACGGAAGCTA CCTCACCAGA 
GACCAGGTCT CTGAGAACGG GCCAGAAGAA AAGGCTCCTT CCAGTCCTGT CGTTCATCCC 
GTAGGCCTCA CCCTCTCCGT CCACCATCTC TACTGCCCGC TCTGCTGGTT GGGCCTCTGG 
TGTATGGACT TGTACATGAT GAACTGTGAA CTTCTAGCCA CGTGTAGCGC CCTTGGGTAC 
TTGGAAGGAG GGACTTACCA CAAGGAGCCG GATTGCCTGG AGAGTGTGAA GGATTTGATC 
CGATACCTGA GGCACGAGGA TGAGACCCGA GATGTGCGGC AGCAGCTGGG AGCTGCACAG 
ATCCTGCAGA GCGACCTCCT GCCAATCCTC ACGCAGCATC GCCAGGACAA GCCTCTCTTC 
GATGCCGTGA TCAGGCTGAT GGTAAATTTG ACACAGCCAG CCTTGCTCTG TTTTGGCAGC 
GTGCCTAAGG ACTCCAGTGT ACGGCACCAT TTTCTGCAGG TTCTAACGTA CCTGCAAGCC 
TACAAAGAGG CCTTTGCCAG TGAGAAGGCA TTTGGAGTCC TCAGCGAGAC CTTGTATGAA 
TTGCTACAGC TGGGCTGGGA GGATCGGCAA GAAGAAGACA ACTTGCTGAT CGAGCGGATC 
CTTCTGCTGG TCAGAAATAT TCTCCATGTC CCGGCCAACC TTGAGCAGGA GAAGAGTATC 
GATGATGATG CCAGCATCCA CGACCGTCTC CTTTGGGCAA TTCACCTCAG TGGCATGGAC 
GACTTGCTCC TCTTCCTGTC CAGCTCATCC GCCGAGCAGC AGTGGAGCCT CCATGTGCTG 
GAGATCATCT CCCTCATGTT CCGAGACCAG ACCCCTGAGC AGCTAGCGGG AGTAGGGCAG 
GGACGCTTGG CTCAGGAGCG AAGCACGGAT GTGGCAGAAT TGGAGGTGCT GCGCCAACGG 
GAGATGGCGG AGAAGAGAGC TCGGGCCCTC CAGCGAGGAA ACAGGCACTC TCGATTTGGG 
GGCTCCTACA TTGTCCAGGG GTTGAAATCT ATTGGGGAGA AGGATGTCGT CTTTCACAAA 
GGCCTTCACA ATCTCCAGAA CTACAGCTCA GATCTGGGAA AGCAGCCCAG GAGGGTGCCC 
AAGCGTCGTC AGGCTGCCCA GGAGCTGTCT GTCCATCGCC GCTCTGTCCT GAATGTGAGA 
CTCTTCCTCA GAGACTTCTG CTCTGAGTTC CTGGAGAACT GCTACAACCC GCTCATGGGC 
GCGGTCAAGG ATCATCTGCT TCGGGAGAGA GCGCAGCAGC ATGACGAGAC TTACTACATG 
TGGGCAATGG CTTTCTTCAT GGCCTTCAAC CGAGCTGCCA CCTTCCGCCC CGGCCTTGTT 
TCTGAGACCC TCAGTATCCG TACCTTTCAC TTTGTGGAGC AGAACCTCAC CAACTACTAC 
GAGATGATGC TGACAGACCG CAAGGAGGCC GCCTCCTGGG CGCGCAGGAT GCACCTGGCC 
CTGAAGGCCT ACCAGGAGCT GCTGGCCACG GTGAACGAGA TGGACATGTG CCCAGATGAG 
GCTGTTAGGG AGAGCAGTCG TATCATCAAA AACAACATTT TCTATATGAT GGAGTACCGA 
GAACTATTCC TGGCGCTCTT TCGAAAGTTT GATGAGAGAT ACCATCCACG CTCATTCCTT 
CGAGACCTGG TGGAAACCAC CCACCTCTTC CTCAAAATGT TGGAGCGCTT TTGCCGGAGC 
CGCGGGAACC TGATGGTGCA GAACAAAAGA AAAAAGAGGA AAAAGAAAAA GAAGGTTCAG 
GACCAGGGTG TTGCTTTCTC ACAAAGCCCC GGGGAGCTGG AGGCCATGTG GCCAGCCCTG 
GCAGAGCAGC TGCTGCAGTG TGCCCAGGAC CCTGAGCTCA GTGTGGACCC CGTCGTTCCC 
TTTGATGCGG CCTCAGAGGT GCCAGTGGAG GAGCAGCGGG TAGAAGCCAT GGTGAGGATC 
CAAGACTGCC TTACGGCTGG CCAGGCCCCG CAAGCCCTGG CCCTCCTGCG GTCTGCCCGG 
GAAGTGTGGC CTGAAGGAAA TGCGTTTGGC TCTCCAGTCA TTTCCCCAGG GGAAGAAATG 
CAGTTGCTGA AACAAATCCT CTCCACGCCC CTTCCCCGGC AGCAGGAGCC AGAAGAAGGA 
GATGCAGAGG AGGAAGAGGA AGAGGAGGAG GAAGAGGAGT TACAGGTGGT CCAGGTGTCA 
GAGAAGGAGT TTAACTTTCT GGAATACCTG AAACGCTTCG CATCCTCAAC CATCGTGCGG 
GCCTACGTGC TTCTCCTGCG GAGCTACAGG CAGAACAGTG CTCACACCAA CCACTGCATC 
GCCAAGATGC TGCACCGGCT GGCCCATGGC CTGGGGATGG AAGCCCTGCT TTTCCAGCTG 
TCCCTGTTCT GCCTCTTCAA TCGGCTGCTT AGTGACCCAG CTGCTGCGGC CTACAAAGAG 
CTAGTGACTT TTGCCAAATA CATCATTGGC AAGTTCTTTG CGTTGGCTGC CGTGAACCAG 
AAAGCGTTTG TGGAGCTGCT GTTCTGGAAG AACACCGCAG TGGTTCGGGA AATGACCCAG 
GGATATGGCT CCCTCGACAG TGGGTCTTCC AGCCACAGAG CTCCTCTGTG GAGCCCTGAG 
GAAGAGGCCC AGCTTCAGGA ACTATACCTC GCCCACAAGG ATGTGGAAGG TCAAGATGTA 
GTGGAAACCA TATTGGCGCA CCTGAAAGTC GTTCCTCGAA CACGCAAGCA GGTCATCCAC 
CACCTGGTCC GGATGGGCCT GGCCGACAGC GTCAAGGAGT TCCAGAAGAG GAAAGGGACC 
CAGATTGTCT TGTGGACGGA GGACCAGGAG CTGGAGTTAC AGCGGCTCTT TGAGGAGTTC 
CGGGACTCTG ATGATGTTCT TGGTCAAATC ATGAAGAATA TCACAGCCAA ACGTTCACGG 
GCTCGAGTAG TGGACAAACT GTTGGCCCTG GGGTTGGTGT CTGAGCGGAG GCAGCTATAC 
AAGAAACGGA GAAAGAAGCT GGCGCCTTCT TGCATGAATG GAGAAAAGTC CCCGAGAGAC 
CCCTGGCAGG AAGATCCGGA AGAGGAAGAC GAACACTTGC CAGAGGACGA AAGTGAAGAT 
GAGGAGAGTG AGGAAGGCTT GCCATCAGGA CAGGGTCAGG GCAGCTCATC TCTCTCTGCT 
GAAAACCTCG GTGAGAGCCT TCGTCAGGAA GGCCTCTCTG CTCCCCTCCT GTGGCTCCAG 
AGCTCCCTGA TCCGAGCAGC AAATGACCGA GAAGAGGATG GCTGCTCCCA GGCAATCCCT 
CTGGTGCCTC TGACAGAGGA AAATGAGGAA GCAATGGAGA ACGAACAGTT TCAGCATCTG 
CTACGCAAGC TAGGGATCCG GCCGCCCAGC TCAGGGCAGG AAACCTTCTG GAGAATTCCA 
GCCAAACTGA GCTCCACCCA GCTTCGGAGG GTGGCTGCTT CTTTGAGTCA GCAAGAAAAC 
GAGGAGGAAA GGGAAGAGGA GCCAGAGCCA GGAGTCCCCG GAGAGCAGGG TCCCAGTGAG 
GAGCACCGGA CAGAAGCCCT GAGAGCCCTT CTGTCAGCCC GTAAGAGGAA AGCAGGCCTG 
GGGCCTACAG AAGAGGAGGC CACTGGGGAG GAAGAATGGA ACTCAGCGCC CAAGAAGCGG 
CAACTGCTGG ACAGCGACGA AGAGGAAGAT GATGAGGGGA GGAGGCAAGC AGTGTCGGGA 
ACGCCAAGAG TCCACAGGAA GAAACGGTTT CAGATTGAGG ATGAGGATGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006513644.1
CDS	1606..5379
Translation	MYRSYLTRDQVSENGSYLTRDQVSENGPEEKAPSSPVVHPVGLTLSVHHLYCPLCWLGLWCMDLYMMNCELLATCSALGYLEGGTYHKEPDCLESVKDLIRYLRHEDETRDVRQQLGAAQILQSDLLPILTQHRQDKPLFDAVIRLMVNLTQPALLCFGSVPKDSSVRHHFLQVLTYLQAYKEAFASEKAFGVLSETLYELLQLGWEDRQEEDNLLIERILLLVRNILHVPANLEQEKSIDDDASIHDRLLWAIHLSGMDDLLLFLSSSSAEQQWSLHVLEIISLMFRDQTPEQLAGVGQGRLAQERSTDVAELEVLRQREMAEKRARALQRGNRHSRFGGSYIVQGLKSIGEKDVVFHKGLHNLQNYSSDLGKQPRRVPKRRQAAQELSVHRRSVLNVRLFLRDFCSEFLENCYNPLMGAVKDHLLRERAQQHDETYYMWAMAFFMAFNRAATFRPGLVSETLSIRTFHFVEQNLTNYYEMMLTDRKEAASWARRMHLALKAYQELLATVNEMDMCPDEAVRESSRIIKNNIFYMMEYRELFLALFRKFDERYHPRSFLRDLVETTHLFLKMLERFCRSRGNLMVQNKRKKRKKKKKVQDQGVAFSQSPGELEAMWPALAEQLLQCAQDPELSVDPVVPFDAASEVPVEEQRVEAMVRIQDCLTAGQAPQALALLRSAREVWPEGNAFGSPVISPGEEMQLLKQILSTPLPRQQEPEEGDAEEEEEEEEEEELQVVQVSEKEFNFLEYLKRFASSTIVRAYVLLLRSYRQNSAHTNHCIAKMLHRLAHGLGMEALLFQLSLFCLFNRLLSDPAAAAYKELVTFAKYIIGKFFALAAVNQKAFVELLFWKNTAVVREMTQGYGSLDSGSSSHRAPLWSPEEEAQLQELYLAHKDVEGQDVVETILAHLKVVPRTRKQVIHHLVRMGLADSVKEFQKRKGTQIVLWTEDQELELQRLFEEFRDSDDVLGQIMKNITAKRSRARVVDKLLALGLVSERRQLYKKRRKKLAPSCMNGEKSPRDPWQEDPEEEDEHLPEDESEDEESEEGLPSGQGQGSSSLSAENLGESLRQEGLSAPLLWLQSSLIRAANDREEDGCSQAIPLVPLTEENEEAMENEQFQHLLRKLGIRPPSSGQETFWRIPAKLSSTQLRRVAASLSQQENEEEREEEPEPGVPGEQGPSEEHRTEALRALLSARKRKAGLGPTEEEATGEEEWNSAPKKRQLLDSDEEEDDEGRRQAVSGTPRVHRKKRFQIEDEDD

Target ORF information:

RefSeq Version	XM_006513581.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus timeless circadian clock 1 (Timeless), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006513581.4

ORF Insert Sequence:

ATGTACAGAA GCTACCTCAC CAGAGACCAG GTCTCTGAGA ACGGAAGCTA CCTCACCAGA 
GACCAGGTCT CTGAGAACGG GCCAGAAGAA AAGGCTCCTT CCAGTCCTGT CGTTCATCCC 
GTAGGCCTCA CCCTCTCCGT CCACCATCTC TACTGCCCGC TCTGCTGGTT GGGCCTCTGG 
TGTATGGACT TGTACATGAT GAACTGTGAA CTTCTAGCCA CGTGTAGCGC CCTTGGGTAC 
TTGGAAGGAG GGACTTACCA CAAGGAGCCG GATTGCCTGG AGAGTGTGAA GGATTTGATC 
CGATACCTGA GGCACGAGGA TGAGACCCGA GATGTGCGGC AGCAGCTGGG AGCTGCACAG 
ATCCTGCAGA GCGACCTCCT GCCAATCCTC ACGCAGCATC GCCAGGACAA GCCTCTCTTC 
GATGCCGTGA TCAGGCTGAT GGTAAATTTG ACACAGCCAG CCTTGCTCTG TTTTGGCAGC 
GTGCCTAAGG ACTCCAGTGT ACGGCACCAT TTTCTGCAGG TTCTAACGTA CCTGCAAGCC 
TACAAAGAGG CCTTTGCCAG TGAGAAGGCA TTTGGAGTCC TCAGCGAGAC CTTGTATGAA 
TTGCTACAGC TGGGCTGGGA GGATCGGCAA GAAGAAGACA ACTTGCTGAT CGAGCGGATC 
CTTCTGCTGG TCAGAAATAT TCTCCATGTC CCGGCCAACC TTGAGCAGGA GAAGAGTATC 
GATGATGATG CCAGCATCCA CGACCGTCTC CTTTGGGCAA TTCACCTCAG TGGCATGGAC 
GACTTGCTCC TCTTCCTGTC CAGCTCATCC GCCGAGCAGC AGTGGAGCCT CCATGTGCTG 
GAGATCATCT CCCTCATGTT CCGAGACCAG ACCCCTGAGC AGCTAGCGGG AGTAGGGCAG 
GGACGCTTGG CTCAGGAGCG AAGCACGGAT GTGGCAGAAT TGGAGGTGCT GCGCCAACGG 
GAGATGGCGG AGAAGAGAGC TCGGGCCCTC CAGCGAGGAA ACAGGCACTC TCGATTTGGG 
GGCTCCTACA TTGTCCAGGG GTTGAAATCT ATTGGGGAGA AGGATGTCGT CTTTCACAAA 
GGCCTTCACA ATCTCCAGAA CTACAGCTCA GATCTGGGAA AGCAGCCCAG GAGGGTGCCC 
AAGCGTCGTC AGGCTGCCCA GGAGCTGTCT GTCCATCGCC GCTCTGTCCT GAATGTGAGA 
CTCTTCCTCA GAGACTTCTG CTCTGAGTTC CTGGAGAACT GCTACAACCC GCTCATGGGC 
GCGGTCAAGG ATCATCTGCT TCGGGAGAGA GCGCAGCAGC ATGACGAGAC TTACTACATG 
TGGGCAATGG CTTTCTTCAT GGCCTTCAAC CGAGCTGCCA CCTTCCGCCC CGGCCTTGTT 
TCTGAGACCC TCAGTATCCG TACCTTTCAC TTTGTGGAGC AGAACCTCAC CAACTACTAC 
GAGATGATGC TGACAGACCG CAAGGAGGCC GCCTCCTGGG CGCGCAGGAT GCACCTGGCC 
CTGAAGGCCT ACCAGGAGCT GCTGGCCACG GTGAACGAGA TGGACATGTG CCCAGATGAG 
GCTGTTAGGG AGAGCAGTCG TATCATCAAA AACAACATTT TCTATATGAT GGAGTACCGA 
GAACTATTCC TGGCGCTCTT TCGAAAGTTT GATGAGAGAT ACCATCCACG CTCATTCCTT 
CGAGACCTGG TGGAAACCAC CCACCTCTTC CTCAAAATGT TGGAGCGCTT TTGCCGGAGC 
CGCGGGAACC TGATGGTGCA GAACAAAAGA AAAAAGAGGA AAAAGAAAAA GAAGGTTCAG 
GACCAGGGTG TTGCTTTCTC ACAAAGCCCC GGGGAGCTGG AGGCCATGTG GCCAGCCCTG 
GCAGAGCAGC TGCTGCAGTG TGCCCAGGAC CCTGAGCTCA GTGTGGACCC CGTCGTTCCC 
TTTGATGCGG CCTCAGAGGT GCCAGTGGAG GAGCAGCGGG TAGAAGCCAT GGTGAGGATC 
CAAGACTGCC TTACGGCTGG CCAGGCCCCG CAAGCCCTGG CCCTCCTGCG GTCTGCCCGG 
GAAGTGTGGC CTGAAGGAAA TGCGTTTGGC TCTCCAGTCA TTTCCCCAGG GGAAGAAATG 
CAGTTGCTGA AACAAATCCT CTCCACGCCC CTTCCCCGGC AGCAGGAGCC AGAAGAAGGA 
GATGCAGAGG AGGAAGAGGA AGAGGAGGAG GAAGAGGAGT TACAGGTGGT CCAGGTGTCA 
GAGAAGGAGT TTAACTTTCT GGAATACCTG AAACGCTTCG CATCCTCAAC CATCGTGCGG 
GCCTACGTGC TTCTCCTGCG GAGCTACAGG CAGAACAGTG CTCACACCAA CCACTGCATC 
GCCAAGATGC TGCACCGGCT GGCCCATGGC CTGGGGATGG AAGCCCTGCT TTTCCAGCTG 
TCCCTGTTCT GCCTCTTCAA TCGGCTGCTT AGTGACCCAG CTGCTGCGGC CTACAAAGAG 
CTAGTGACTT TTGCCAAATA CATCATTGGC AAGTTCTTTG CGTTGGCTGC CGTGAACCAG 
AAAGCGTTTG TGGAGCTGCT GTTCTGGAAG AACACCGCAG TGGTTCGGGA AATGACCCAG 
GGATATGGCT CCCTCGACAG TGGGTCTTCC AGCCACAGAG CTCCTCTGTG GAGCCCTGAG 
GAAGAGGCCC AGCTTCAGGA ACTATACCTC GCCCACAAGG ATGTGGAAGG TCAAGATGTA 
GTGGAAACCA TATTGGCGCA CCTGAAAGTC GTTCCTCGAA CACGCAAGCA GGTCATCCAC 
CACCTGGTCC GGATGGGCCT GGCCGACAGC GTCAAGGAGT TCCAGAAGAG GAAAGGGACC 
CAGATTGTCT TGTGGACGGA GGACCAGGAG CTGGAGTTAC AGCGGCTCTT TGAGGAGTTC 
CGGGACTCTG ATGATGTTCT TGGTCAAATC ATGAAGAATA TCACAGCCAA ACGTTCACGG 
GCTCGAGTAG TGGACAAACT GTTGGCCCTG GGGTTGGTGT CTGAGCGGAG GCAGCTATAC 
AAGAAACGGA GAAAGAAGCT GGCGCCTTCT TGCATGAATG GAGAAAAGTC CCCGAGAGAC 
CCCTGGCAGG AAGATCCGGA AGAGGAAGAC GAACACTTGC CAGAGGACGA AAGTGAAGAT 
GAGGAGAGTG AGGAAGGCTT GCCATCAGGA CAGGGTCAGG GCAGCTCATC TCTCTCTGCT 
GAAAACCTCG GTGAGAGCCT TCGTCAGGAA GGCCTCTCTG CTCCCCTCCT GTGGCTCCAG 
AGCTCCCTGA TCCGAGCAGC AAATGACCGA GAAGAGGATG GCTGCTCCCA GGCAATCCCT 
CTGGTGCCTC TGACAGAGGA AAATGAGGAA GCAATGGAGA ACGAACAGTT TCAGCATCTG 
CTACGCAAGC TAGGGATCCG GCCGCCCAGC TCAGGGCAGG AAACCTTCTG GAGAATTCCA 
GCCAAACTGA GCTCCACCCA GCTTCGGAGG GTGGCTGCTT CTTTGAGTCA GCAAGAAAAC 
GAGGAGGAAA GGGAAGAGGA GCCAGAGCCA GGAGTCCCCG GAGAGCAGGG TCCCAGTGAG 
GAGCACCGGA CAGAAGCCCT GAGAGCCCTT CTGTCAGCCC GTAAGAGGAA AGCAGGCCTG 
GGGCCTACAG AAGAGGAGGC CACTGGGGAG GAAGAATGGA ACTCAGCGCC CAAGAAGCGG 
CAACTGCTGG ACAGCGACGA AGAGGAAGAT GATGAGGGGA GGAGGCAAGC AGTGTCGGGA 
ACGCCAAGAG TCCACAGGAA GAAACGGTTT CAGATTGAGG ATGAGGATGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Timeless ( XM_006513582.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006513645.1 Mus musculus timeless circadian clock 1 (Timeless), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu44456
Clone ID Related Accession (Same CDS sequence)	XM_006513582.2 , XM_006513582.1
Accession Version	XM_006513582.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein timeless homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006513582.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACAGAA GCTACCTCAC CAGAGACCAG GTCTCTGAGA ACGGAAGCTA CCTCACCAGA 
GACCAGGTCT CTGAGAACGG GCCAGAAGAA AAGGCTCCTT CCAGTCCTGT CGTTCATCCC 
GTAGGCCTCA CCCTCTCCGT CCACCATCTC TACTGCCCGC TCTGCTGGTT GGGCCTCTGG 
TGTATGGACT TGTACATGAT GAACTGTGAA CTTCTAGCCA CGTGTAGCGC CCTTGGGTAC 
TTGGAAGGAG GGACTTACCA CAAGGAGCCG GATTGCCTGG AGAGTGTGAA GGATTTGATC 
CGATACCTGA GGCACGAGGA TGAGACCCGA GATGTGCGGC AGCAGCTGGG AGCTGCACAG 
ATCCTGCAGA GCGACCTCCT GCCAATCCTC ACGCAGCATC GCCAGGACAA GCCTCTCTTC 
GATGCCGTGA TCAGGCTGAT GGTAAATTTG ACACAGCCAG CCTTGCTCTG TTTTGGCAGC 
GTGCCTAAGG ACTCCAGTGT ACGGCACCAT TTTCTGCAGG TTCTAACGTA CCTGCAAGCC 
TACAAAGAGG CCTTTGCCAG TGAGAAGGCA TTTGGAGTCC TCAGCGAGAC CTTGTATGAA 
TTGCTACAGC TGGGCTGGGA GGATCGGCAA GAAGAAGACA ACTTGCTGAT CGAGCGGATC 
CTTCTGCTGG TCAGAAATAT TCTCCATGTC CCGGCCAACC TTGAGCAGGA GAAGAGTATC 
GATGATGATG CCAGCATCCA CGACCGTCTC CTTTGGGCAA TTCACCTCAG TGGCATGGAC 
GACTTGCTCC TCTTCCTGTC CAGCTCATCC GCCGAGCAGC AGTGGAGCCT CCATGTGCTG 
GAGATCATCT CCCTCATGTT CCGAGACCAG ACCCCTGAGC AGCTAGCGGG AGTAGGGCAG 
GGACGCTTGG CTCAGGAGCG AAGCACGGAT GTGGCAGAAT TGGAGGTGCT GCGCCAACGG 
GAGATGGCGG AGAAGAGAGC TCGGGCCCTC CAGCGAGGAA ACAGGCACTC TCGATTTGGG 
GGCTCCTACA TTGTCCAGGG GTTGAAATCT ATTGGGGAGA AGGATGTCGT CTTTCACAAA 
GGCCTTCACA ATCTCCAGAA CTACAGCTCA GATCTGGGAA AGCAGCCCAG GAGGGTGCCC 
AAGCGTCGTC AGGCTGCCCA GGAGCTGTCT GTCCATCGCC GCTCTGTCCT GAATGTGAGA 
CTCTTCCTCA GAGACTTCTG CTCTGAGTTC CTGGAGAACT GCTACAACCC GCTCATGGGC 
GCGGTCAAGG ATCATCTGCT TCGGGAGAGA GCGCAGCAGC ATGACGAGAC TTACTACATG 
TGGGCAATGG CTTTCTTCAT GGCCTTCAAC CGAGCTGCCA CCTTCCGCCC CGGCCTTGTT 
TCTGAGACCC TCAGTATCCG TACCTTTCAC TTTGTGGAGC AGAACCTCAC CAACTACTAC 
GAGATGATGC TGACAGACCG CAAGGAGGCC GCCTCCTGGG CGCGCAGGAT GCACCTGGCC 
CTGAAGGCCT ACCAGGAGCT GCTGGCCACG GTGAACGAGA TGGACATGTG CCCAGATGAG 
GCTGTTAGGG AGAGCAGTCG TATCATCAAA AACAACATTT TCTATATGAT GGAGTACCGA 
GAACTATTCC TGGCGCTCTT TCGAAAGTTT GATGAGAGAT ACCATCCACG CTCATTCCTT 
CGAGACCTGG TGGAAACCAC CCACCTCTTC CTCAAAATGT TGGAGCGCTT TTGCCGGAGC 
CGCGGGAACC TGATGGTGCA GAACAAAAGA AAAAAGAGGA AAAAGAAAAA GAAGGTTCAG 
GACCAGGGTG TTGCTTTCTC ACAAAGCCCC GGGGAGCTGG AGGCCATGTG GCCAGCCCTG 
GCAGAGCAGC TGCTGCAGTG TGCCCAGGAC CCTGAGCTCA GTGTGGACCC CGTCGTTCCC 
TTTGATGCGG CCTCAGAGGT GCCAGTGGAG GAGCAGCGGG TAGAAGCCAT GGTGAGGATC 
CAAGACTGCC TTACGGCTGG CCAGGCCCCG CAAGCCCTGG CCCTCCTGCG GTCTGCCCGG 
GAAGTGTGGC CTGAAGGAAA TGCGTTTGGC TCTCCAGTCA TTTCCCCAGG GGAAGAAATG 
CAGTTGCTGA AACAAATCCT CTCCACGCCC CTTCCCCGGC AGCAGGAGCC AGAAGAAGGA 
GATGCAGAGG AGGAAGAGGA AGAGGAGGAG GAAGAGGAGT TACAGGTGGT CCAGGTGTCA 
GAGAAGGAGT TTAACTTTCT GGAATACCTG AAACGCTTCG CATCCTCAAC CATCGTGCGG 
GCCTACGTGC TTCTCCTGCG GAGCTACAGG CAGAACAGTG CTCACACCAA CCACTGCATC 
GCCAAGATGC TGCACCGGCT GGCCCATGGC CTGGGGATGG AAGCCCTGCT TTTCCAGCTG 
TCCCTGTTCT GCCTCTTCAA TCGGCTGCTT AGTGACCCAG CTGCTGCGGC CTACAAAGAG 
CTAGTGACTT TTGCCAAATA CATCATTGGC AAGTTCTTTG CGTTGGCTGC CGTGAACCAG 
AAAGCGTTTG TGGAGCTGCT GTTCTGGAAG AACACCGCAG TGGTTCGGGA AATGACCCAG 
GGATATGGCT CCCTCGACAG TGGGTCTTCC AGCCACAGAG CTCCTCTGTG GAGCCCTGAG 
GAAGAGGCCC AGCTTCAGGA ACTATACCTC GCCCACAAGG ATGTGGAAGG TCAAGATGTA 
GTGGAAACCA TATTGGCGCA CCTGAAAGTC GTTCCTCGAA CACGCAAGCA GGTCATCCAC 
CACCTGGTCC GGATGGGCCT GGCCGACAGC GTCAAGGAGT TCCAGAAGAG GAAAGGGACC 
CAGATTGTCT TGTGGACGGA GGACCAGGAG CTGGAGTTAC AGCGGCTCTT TGAGGAGTTC 
CGGGACTCTG ATGATGTTCT TGGTCAAATC ATGAAGAATA TCACAGCCAA ACGTTCACGG 
GCTCGAGTAG TGGACAAACT GTTGGCCCTG GGGTTGGTGT CTGAGCGGAG GCAGCTATAC 
AAGAAACGGA GAAAGAAGCT GGCGCCTTCT TGCATGCAGA ATGGAGAAAA GTCCCCGAGA 
GACCCCTGGC AGGAAGATCC GGAAGAGGAA GACGAACACT TGCCAGAGGA CGAAAGTGAA 
GATGAGGAGA GTGAGGAAGG CTTGCCATCA GGACAGGGTC AGGGCAGCTC ATCTCTCTCT 
GCTGAAAACC TCGGTGAGAG CCTTCGTCAG GAAGGCCTCT CTGCTCCCCT CCTGTGGCTC 
CAGAGCTCCC TGATCCGAGC AGCAAATGAC CGAGAAGAGG ATGGCTGCTC CCAGGCAATC 
CCTCTGGTGC CTCTGACAGA GGAAAATGAG GAAGCAATGG AGAACGAACA GTTTCAGCAT 
CTGCTACGCA AGCTAGGGAT CCGGCCGCCC AGCTCAGGGC AGGAAACCTT CTGGAGAATT 
CCAGCCAAAC TGAGCTCCAC CCAGCTTCGG AGGGTGGCTG CTTCTTTGAG TCAGCAAGAA 
AACGAGGAGG AAAGGGAAGA GGAGCCAGAG CCAGGAGTCC CCGGAGAGCA GGGTCCCAGT 
GAGGAGCACC GGACAGAAGC CCTGAGAGCC CTTCTGTCAG CCCGTAAGAG GAAAGCAGGC 
CTGGGGCCTA CAGAAGAGGA GGCCACTGGG GAGGAAGAAT GGAACTCAGC GCCCAAGAAG 
CGGCAACTGC TGGACAGCGA CGAAGAGGAA GATGATGAGG GGAGGAGGCA AGTGTCGGGA 
ACGCCAAGAG TCCACAGGAA GAAACGGTTT CAGATTGAGG ATGAGGATGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006513645.1
CDS	1606..5379
Translation	MYRSYLTRDQVSENGSYLTRDQVSENGPEEKAPSSPVVHPVGLTLSVHHLYCPLCWLGLWCMDLYMMNCELLATCSALGYLEGGTYHKEPDCLESVKDLIRYLRHEDETRDVRQQLGAAQILQSDLLPILTQHRQDKPLFDAVIRLMVNLTQPALLCFGSVPKDSSVRHHFLQVLTYLQAYKEAFASEKAFGVLSETLYELLQLGWEDRQEEDNLLIERILLLVRNILHVPANLEQEKSIDDDASIHDRLLWAIHLSGMDDLLLFLSSSSAEQQWSLHVLEIISLMFRDQTPEQLAGVGQGRLAQERSTDVAELEVLRQREMAEKRARALQRGNRHSRFGGSYIVQGLKSIGEKDVVFHKGLHNLQNYSSDLGKQPRRVPKRRQAAQELSVHRRSVLNVRLFLRDFCSEFLENCYNPLMGAVKDHLLRERAQQHDETYYMWAMAFFMAFNRAATFRPGLVSETLSIRTFHFVEQNLTNYYEMMLTDRKEAASWARRMHLALKAYQELLATVNEMDMCPDEAVRESSRIIKNNIFYMMEYRELFLALFRKFDERYHPRSFLRDLVETTHLFLKMLERFCRSRGNLMVQNKRKKRKKKKKVQDQGVAFSQSPGELEAMWPALAEQLLQCAQDPELSVDPVVPFDAASEVPVEEQRVEAMVRIQDCLTAGQAPQALALLRSAREVWPEGNAFGSPVISPGEEMQLLKQILSTPLPRQQEPEEGDAEEEEEEEEEEELQVVQVSEKEFNFLEYLKRFASSTIVRAYVLLLRSYRQNSAHTNHCIAKMLHRLAHGLGMEALLFQLSLFCLFNRLLSDPAAAAYKELVTFAKYIIGKFFALAAVNQKAFVELLFWKNTAVVREMTQGYGSLDSGSSSHRAPLWSPEEEAQLQELYLAHKDVEGQDVVETILAHLKVVPRTRKQVIHHLVRMGLADSVKEFQKRKGTQIVLWTEDQELELQRLFEEFRDSDDVLGQIMKNITAKRSRARVVDKLLALGLVSERRQLYKKRRKKLAPSCMQNGEKSPRDPWQEDPEEEDEHLPEDESEDEESEEGLPSGQGQGSSSLSAENLGESLRQEGLSAPLLWLQSSLIRAANDREEDGCSQAIPLVPLTEENEEAMENEQFQHLLRKLGIRPPSSGQETFWRIPAKLSSTQLRRVAASLSQQENEEEREEEPEPGVPGEQGPSEEHRTEALRALLSARKRKAGLGPTEEEATGEEEWNSAPKKRQLLDSDEEEDDEGRRQVSGTPRVHRKKRFQIEDEDD

Target ORF information:

RefSeq Version	XM_006513582.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus timeless circadian clock 1 (Timeless), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006513582.2

ORF Insert Sequence:

ATGTACAGAA GCTACCTCAC CAGAGACCAG GTCTCTGAGA ACGGAAGCTA CCTCACCAGA 
GACCAGGTCT CTGAGAACGG GCCAGAAGAA AAGGCTCCTT CCAGTCCTGT CGTTCATCCC 
GTAGGCCTCA CCCTCTCCGT CCACCATCTC TACTGCCCGC TCTGCTGGTT GGGCCTCTGG 
TGTATGGACT TGTACATGAT GAACTGTGAA CTTCTAGCCA CGTGTAGCGC CCTTGGGTAC 
TTGGAAGGAG GGACTTACCA CAAGGAGCCG GATTGCCTGG AGAGTGTGAA GGATTTGATC 
CGATACCTGA GGCACGAGGA TGAGACCCGA GATGTGCGGC AGCAGCTGGG AGCTGCACAG 
ATCCTGCAGA GCGACCTCCT GCCAATCCTC ACGCAGCATC GCCAGGACAA GCCTCTCTTC 
GATGCCGTGA TCAGGCTGAT GGTAAATTTG ACACAGCCAG CCTTGCTCTG TTTTGGCAGC 
GTGCCTAAGG ACTCCAGTGT ACGGCACCAT TTTCTGCAGG TTCTAACGTA CCTGCAAGCC 
TACAAAGAGG CCTTTGCCAG TGAGAAGGCA TTTGGAGTCC TCAGCGAGAC CTTGTATGAA 
TTGCTACAGC TGGGCTGGGA GGATCGGCAA GAAGAAGACA ACTTGCTGAT CGAGCGGATC 
CTTCTGCTGG TCAGAAATAT TCTCCATGTC CCGGCCAACC TTGAGCAGGA GAAGAGTATC 
GATGATGATG CCAGCATCCA CGACCGTCTC CTTTGGGCAA TTCACCTCAG TGGCATGGAC 
GACTTGCTCC TCTTCCTGTC CAGCTCATCC GCCGAGCAGC AGTGGAGCCT CCATGTGCTG 
GAGATCATCT CCCTCATGTT CCGAGACCAG ACCCCTGAGC AGCTAGCGGG AGTAGGGCAG 
GGACGCTTGG CTCAGGAGCG AAGCACGGAT GTGGCAGAAT TGGAGGTGCT GCGCCAACGG 
GAGATGGCGG AGAAGAGAGC TCGGGCCCTC CAGCGAGGAA ACAGGCACTC TCGATTTGGG 
GGCTCCTACA TTGTCCAGGG GTTGAAATCT ATTGGGGAGA AGGATGTCGT CTTTCACAAA 
GGCCTTCACA ATCTCCAGAA CTACAGCTCA GATCTGGGAA AGCAGCCCAG GAGGGTGCCC 
AAGCGTCGTC AGGCTGCCCA GGAGCTGTCT GTCCATCGCC GCTCTGTCCT GAATGTGAGA 
CTCTTCCTCA GAGACTTCTG CTCTGAGTTC CTGGAGAACT GCTACAACCC GCTCATGGGC 
GCGGTCAAGG ATCATCTGCT TCGGGAGAGA GCGCAGCAGC ATGACGAGAC TTACTACATG 
TGGGCAATGG CTTTCTTCAT GGCCTTCAAC CGAGCTGCCA CCTTCCGCCC CGGCCTTGTT 
TCTGAGACCC TCAGTATCCG TACCTTTCAC TTTGTGGAGC AGAACCTCAC CAACTACTAC 
GAGATGATGC TGACAGACCG CAAGGAGGCC GCCTCCTGGG CGCGCAGGAT GCACCTGGCC 
CTGAAGGCCT ACCAGGAGCT GCTGGCCACG GTGAACGAGA TGGACATGTG CCCAGATGAG 
GCTGTTAGGG AGAGCAGTCG TATCATCAAA AACAACATTT TCTATATGAT GGAGTACCGA 
GAACTATTCC TGGCGCTCTT TCGAAAGTTT GATGAGAGAT ACCATCCACG CTCATTCCTT 
CGAGACCTGG TGGAAACCAC CCACCTCTTC CTCAAAATGT TGGAGCGCTT TTGCCGGAGC 
CGCGGGAACC TGATGGTGCA GAACAAAAGA AAAAAGAGGA AAAAGAAAAA GAAGGTTCAG 
GACCAGGGTG TTGCTTTCTC ACAAAGCCCC GGGGAGCTGG AGGCCATGTG GCCAGCCCTG 
GCAGAGCAGC TGCTGCAGTG TGCCCAGGAC CCTGAGCTCA GTGTGGACCC CGTCGTTCCC 
TTTGATGCGG CCTCAGAGGT GCCAGTGGAG GAGCAGCGGG TAGAAGCCAT GGTGAGGATC 
CAAGACTGCC TTACGGCTGG CCAGGCCCCG CAAGCCCTGG CCCTCCTGCG GTCTGCCCGG 
GAAGTGTGGC CTGAAGGAAA TGCGTTTGGC TCTCCAGTCA TTTCCCCAGG GGAAGAAATG 
CAGTTGCTGA AACAAATCCT CTCCACGCCC CTTCCCCGGC AGCAGGAGCC AGAAGAAGGA 
GATGCAGAGG AGGAAGAGGA AGAGGAGGAG GAAGAGGAGT TACAGGTGGT CCAGGTGTCA 
GAGAAGGAGT TTAACTTTCT GGAATACCTG AAACGCTTCG CATCCTCAAC CATCGTGCGG 
GCCTACGTGC TTCTCCTGCG GAGCTACAGG CAGAACAGTG CTCACACCAA CCACTGCATC 
GCCAAGATGC TGCACCGGCT GGCCCATGGC CTGGGGATGG AAGCCCTGCT TTTCCAGCTG 
TCCCTGTTCT GCCTCTTCAA TCGGCTGCTT AGTGACCCAG CTGCTGCGGC CTACAAAGAG 
CTAGTGACTT TTGCCAAATA CATCATTGGC AAGTTCTTTG CGTTGGCTGC CGTGAACCAG 
AAAGCGTTTG TGGAGCTGCT GTTCTGGAAG AACACCGCAG TGGTTCGGGA AATGACCCAG 
GGATATGGCT CCCTCGACAG TGGGTCTTCC AGCCACAGAG CTCCTCTGTG GAGCCCTGAG 
GAAGAGGCCC AGCTTCAGGA ACTATACCTC GCCCACAAGG ATGTGGAAGG TCAAGATGTA 
GTGGAAACCA TATTGGCGCA CCTGAAAGTC GTTCCTCGAA CACGCAAGCA GGTCATCCAC 
CACCTGGTCC GGATGGGCCT GGCCGACAGC GTCAAGGAGT TCCAGAAGAG GAAAGGGACC 
CAGATTGTCT TGTGGACGGA GGACCAGGAG CTGGAGTTAC AGCGGCTCTT TGAGGAGTTC 
CGGGACTCTG ATGATGTTCT TGGTCAAATC ATGAAGAATA TCACAGCCAA ACGTTCACGG 
GCTCGAGTAG TGGACAAACT GTTGGCCCTG GGGTTGGTGT CTGAGCGGAG GCAGCTATAC 
AAGAAACGGA GAAAGAAGCT GGCGCCTTCT TGCATGCAGA ATGGAGAAAA GTCCCCGAGA 
GACCCCTGGC AGGAAGATCC GGAAGAGGAA GACGAACACT TGCCAGAGGA CGAAAGTGAA 
GATGAGGAGA GTGAGGAAGG CTTGCCATCA GGACAGGGTC AGGGCAGCTC ATCTCTCTCT 
GCTGAAAACC TCGGTGAGAG CCTTCGTCAG GAAGGCCTCT CTGCTCCCCT CCTGTGGCTC 
CAGAGCTCCC TGATCCGAGC AGCAAATGAC CGAGAAGAGG ATGGCTGCTC CCAGGCAATC 
CCTCTGGTGC CTCTGACAGA GGAAAATGAG GAAGCAATGG AGAACGAACA GTTTCAGCAT 
CTGCTACGCA AGCTAGGGAT CCGGCCGCCC AGCTCAGGGC AGGAAACCTT CTGGAGAATT 
CCAGCCAAAC TGAGCTCCAC CCAGCTTCGG AGGGTGGCTG CTTCTTTGAG TCAGCAAGAA 
AACGAGGAGG AAAGGGAAGA GGAGCCAGAG CCAGGAGTCC CCGGAGAGCA GGGTCCCAGT 
GAGGAGCACC GGACAGAAGC CCTGAGAGCC CTTCTGTCAG CCCGTAAGAG GAAAGCAGGC 
CTGGGGCCTA CAGAAGAGGA GGCCACTGGG GAGGAAGAAT GGAACTCAGC GCCCAAGAAG 
CGGCAACTGC TGGACAGCGA CGAAGAGGAA GATGATGAGG GGAGGAGGCA AGTGTCGGGA 
ACGCCAAGAG TCCACAGGAA GAAACGGTTT CAGATTGAGG ATGAGGATGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Timeless ( XM_030245049.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030100909.1 Mus musculus timeless circadian clock 1 (Timeless), transcript variant X4, mRNA.

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CloneID	OMu85427
Clone ID Related Accession (Same CDS sequence)	XM_030245049.1
Accession Version	XM_030245049.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein timeless homolog isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACAGAA GCTACCTCAC CAGAGACCAG GTCTCTGAGA ACGGAAGCTA CCTCACCAGA 
GACCAGGTCT CTGAGAACGG GCCAGAAGAA AAGGCTCCTT CCAGTCCTGT CGTTCATCCC 
GTAGGCCTCA CCCTCTCCGT CCACCATCTC TACTGCCCGC TCTGCTGGTT GGGCCTCTGG 
TGTATGGACT TGTACATGAT GAACTGTGAA CTTCTAGCCA CGTGTAGCGC CCTTGGGTAC 
TTGGAAGGAG GGACTTACCA CAAGGAGCCG GATTGCCTGG AGAGTGTGAA GGATTTGATC 
CGATACCTGA GGCACGAGGA TGAGACCCGA GATGTGCGGC AGCAGCTGGG AGCTGCACAG 
ATCCTGCAGA GCGACCTCCT GCCAATCCTC ACGCAGCATC GCCAGGACAA GCCTCTCTTC 
GATGCCGTGA TCAGGCTGAT GGTAAATTTG ACACAGCCAG CCTTGCTCTG TTTTGGCAGC 
GTGCCTAAGG ACTCCAGTGT ACGGCACCAT TTTCTGCAGG TTCTAACGTA CCTGCAAGCC 
TACAAAGAGG CCTTTGCCAG TGAGAAGGCA TTTGGAGTCC TCAGCGAGAC CTTGTATGAA 
TTGCTACAGC TGGGCTGGGA GGATCGGCAA GAAGAAGACA ACTTGCTGAT CGAGCGGATC 
CTTCTGCTGG TCAGAAATAT TCTCCATGTC CCGGCCAACC TTGAGCAGGA GAAGAGTATC 
GATGATGATG CCAGCATCCA CGACCGTCTC CTTTGGGCAA TTCACCTCAG TGGCATGGAC 
GACTTGCTCC TCTTCCTGTC CAGCTCATCC GCCGAGCAGC AGTGGAGCCT CCATGTGCTG 
GAGATCATCT CCCTCATGTT CCGAGACCAG ACCCCTGAGC AGCTAGCGGG AGTAGGGCAG 
GGACGCTTGG CTCAGGAGCG AAGCACGGAT GTGGCAGAAT TGGAGGTGCT GCGCCAACGG 
GAGATGGCGG AGAAGAGAGC TCGGGCCCTC CAGCGAGGAA ACAGGCACTC TCGATTTGGG 
GGCTCCTACA TTGTCCAGGG GTTGAAATCT ATTGGGGAGA AGGATGTCGT CTTTCACAAA 
GGCCTTCACA ATCTCCAGAA CTACAGCTCA GATCTGGGAA AGCAGCCCAG GAGGGTGCCC 
AAGCGTCGTC AGGCTGCCCA GGAGCTGTCT GTCCATCGCC GCTCTGTCCT GAATGTGAGA 
CTCTTCCTCA GAGACTTCTG CTCTGAGTTC CTGGAGAACT GCTACAACCC GCTCATGGGC 
GCGGTCAAGG ATCATCTGCT TCGGGAGAGA GCGCAGCAGC ATGACGAGAC TTACTACATG 
TGGGCAATGG CTTTCTTCAT GGCCTTCAAC CGAGCTGCCA CCTTCCGCCC CGGCCTTGTT 
TCTGAGACCC TCAGTATCCG TACCTTTCAC TTTGTGGAGC AGAACCTCAC CAACTACTAC 
GAGATGATGC TGACAGACCG CAAGGAGGCC GCCTCCTGGG CGCGCAGGAT GCACCTGGCC 
CTGAAGGCCT ACCAGGAGCT GCTGGCCACG GTGAACGAGA TGGACATGTG CCCAGATGAG 
GCTGTTAGGG AGAGCAGTCG TATCATCAAA AACAACATTT TCTATATGAT GGAGTACCGA 
GAACTATTCC TGGCGCTCTT TCGAAAGTTT GATGAGAGAT ACCATCCACG CTCATTCCTT 
CGAGACCTGG TGGAAACCAC CCACCTCTTC CTCAAAATGT TGGAGCGCTT TTGCCGGAGC 
CGCGGGAACC TGATGGTGCA GAACAAAAGA AAAAAGAGGA AAAAGAAAAA GAAGGTTCAG 
GACCAGGGTG TTGCTTTCTC ACAAAGCCCC GGGGAGCTGG AGGCCATGTG GCCAGCCCTG 
GCAGAGCAGC TGCTGCAGTG TGCCCAGGAC CCTGAGCTCA GTGTGGACCC CGTCGTTCCC 
TTTGATGCGG CCTCAGAGGT GCCAGTGGAG GAGCAGCGGG TAGAAGCCAT GGTGAGGATC 
CAAGACTGCC TTACGGCTGG CCAGGCCCCG CAAGCCCTGG CCCTCCTGCG GTCTGCCCGG 
GAAGTGTGGC CTGAAGGAAA TGCGTTTGGC TCTCCAGTCA TTTCCCCAGG GGAAGAAATG 
CAGTTGCTGA AACAAATCCT CTCCACGCCC CTTCCCCGGC AGCAGGAGCC AGAAGAAGGA 
GATGCAGAGG AGGAAGAGGA AGAGGAGGAG GAAGAGGAGT TACAGGTGGT CCAGGTGTCA 
GAGAAGGAGT TTAACTTTCT GGAATACCTG AAACGCTTCG CATCCTCAAC CATCGTGCGG 
GCCTACGTGC TTCTCCTGCG GAGCTACAGG CAGAACAGTG CTCACACCAA CCACTGCATC 
GCCAAGATGC TGCACCGGCT GGCCCATGGC CTGGGGATGG AAGCCCTGCT TTTCCAGCTG 
TCCCTGTTCT GCCTCTTCAA TCGGCTGCTT AGTGACCCAG CTGCTGCGGC CTACAAAGAG 
CTAGTGACTT TTGCCAAATA CATCATTGGC AAGTTCTTTG CGTTGGCTGC CGTGAACCAG 
AAAGCGTTTG TGGAGCTGCT GTTCTGGAAG AACACCGCAG TGGTTCGGGA AATGACCCAG 
GGATATGGCT CCCTCGACAG TGGGTCTTCC AGCCACAGAG CTCCTCTGTG GAGCCCTGAG 
GAAGAGGCCC AGCTTCAGGA ACTATACCTC GCCCACAAGG ATGTGGAAGG TCAAGATGTA 
GTGGAAACCA TATTGGCGCA CCTGAAAGTC GTTCCTCGAA CACGCAAGCA GGTCATCCAC 
CACCTGGTCC GGATGGGCCT GGCCGACAGC GTCAAGGAGT TCCAGAAGAG GAAAGGGACC 
CAGATTGTCT TGTGGACGGA GGACCAGGAG CTGGAGTTAC AGCGGCTCTT TGAGGAGTTC 
CGGGACTCTG ATGATGTTCT TGGTCAAATC ATGAAGAATA TCACAGCCAA ACGTTCACGG 
GCTCGAGTAG TGGACAAACT GTTGGCCCTG GGGTTGGTGT CTGAGCGGAG GCAGCTATAC 
AAGAAACGGA GAAAGAAGCT GGCGCCTTCT TGCATGAATG GAGAAAAGTC CCCGAGAGAC 
CCCTGGCAGG AAGATCCGGA AGAGGAAGAC GAACACTTGC CAGAGGACGA AAGTGAAGAT 
GAGGAGAGTG AGGAAGGCTT GCCATCAGGA CAGGGTCAGG GCAGCTCATC TCTCTCTGCT 
GAAAACCTCG GTGAGAGCCT TCGTCAGGAA GGCCTCTCTG CTCCCCTCCT GTGGCTCCAG 
AGCTCCCTGA TCCGAGCAGC AAATGACCGA GAAGAGGATG GCTGCTCCCA GGCAATCCCT 
CTGGTGCCTC TGACAGAGGA AAATGAGGAA GCAATGGAGA ACGAACAGTT TCAGCATCTG 
CTACGCAAGC TAGGGATCCG GCCGCCCAGC TCAGGGCAGG AAACCTTCTG GAGAATTCCA 
GCCAAACTGA GCTCCACCCA GCTTCGGAGG GTGGCTGCTT CTTTGAGTCA GCAAGAAAAC 
GAGGAGGAAA GGGAAGAGGA GCCAGAGCCA GGAGTCCCCG GAGAGCAGGG TCCCAGTGAG 
GAGCACCGGA CAGAAGCCCT GAGAGCCCTT CTGTCAGCCC GTAAGAGGAA AGCAGGCCTG 
GGGCCTACAG AAGAGGAGGC CACTGGGGAG GAAGAATGGA ACTCAGCGCC CAAGAAGCGG 
CAACTGCTGG ACAGCGACGA AGAGGAAGAT GATGAGGGGA GGAGGCAAGT GTCGGGAACG 
CCAAGAGTCC ACAGGAAGAA ACGGTTTCAG ATTGAGGATG AGGATGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030100909.1
CDS	1607..5377
Translation	MYRSYLTRDQVSENGSYLTRDQVSENGPEEKAPSSPVVHPVGLTLSVHHLYCPLCWLGLWCMDLYMMNCELLATCSALGYLEGGTYHKEPDCLESVKDLIRYLRHEDETRDVRQQLGAAQILQSDLLPILTQHRQDKPLFDAVIRLMVNLTQPALLCFGSVPKDSSVRHHFLQVLTYLQAYKEAFASEKAFGVLSETLYELLQLGWEDRQEEDNLLIERILLLVRNILHVPANLEQEKSIDDDASIHDRLLWAIHLSGMDDLLLFLSSSSAEQQWSLHVLEIISLMFRDQTPEQLAGVGQGRLAQERSTDVAELEVLRQREMAEKRARALQRGNRHSRFGGSYIVQGLKSIGEKDVVFHKGLHNLQNYSSDLGKQPRRVPKRRQAAQELSVHRRSVLNVRLFLRDFCSEFLENCYNPLMGAVKDHLLRERAQQHDETYYMWAMAFFMAFNRAATFRPGLVSETLSIRTFHFVEQNLTNYYEMMLTDRKEAASWARRMHLALKAYQELLATVNEMDMCPDEAVRESSRIIKNNIFYMMEYRELFLALFRKFDERYHPRSFLRDLVETTHLFLKMLERFCRSRGNLMVQNKRKKRKKKKKVQDQGVAFSQSPGELEAMWPALAEQLLQCAQDPELSVDPVVPFDAASEVPVEEQRVEAMVRIQDCLTAGQAPQALALLRSAREVWPEGNAFGSPVISPGEEMQLLKQILSTPLPRQQEPEEGDAEEEEEEEEEEELQVVQVSEKEFNFLEYLKRFASSTIVRAYVLLLRSYRQNSAHTNHCIAKMLHRLAHGLGMEALLFQLSLFCLFNRLLSDPAAAAYKELVTFAKYIIGKFFALAAVNQKAFVELLFWKNTAVVREMTQGYGSLDSGSSSHRAPLWSPEEEAQLQELYLAHKDVEGQDVVETILAHLKVVPRTRKQVIHHLVRMGLADSVKEFQKRKGTQIVLWTEDQELELQRLFEEFRDSDDVLGQIMKNITAKRSRARVVDKLLALGLVSERRQLYKKRRKKLAPSCMNGEKSPRDPWQEDPEEEDEHLPEDESEDEESEEGLPSGQGQGSSSLSAENLGESLRQEGLSAPLLWLQSSLIRAANDREEDGCSQAIPLVPLTEENEEAMENEQFQHLLRKLGIRPPSSGQETFWRIPAKLSSTQLRRVAASLSQQENEEEREEEPEPGVPGEQGPSEEHRTEALRALLSARKRKAGLGPTEEEATGEEEWNSAPKKRQLLDSDEEEDDEGRRQVSGTPRVHRKKRFQIEDEDD

Target ORF information:

RefSeq Version	XM_030245049.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus timeless circadian clock 1 (Timeless), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030245049.1

ORF Insert Sequence:

ATGTACAGAA GCTACCTCAC CAGAGACCAG GTCTCTGAGA ACGGAAGCTA CCTCACCAGA 
GACCAGGTCT CTGAGAACGG GCCAGAAGAA AAGGCTCCTT CCAGTCCTGT CGTTCATCCC 
GTAGGCCTCA CCCTCTCCGT CCACCATCTC TACTGCCCGC TCTGCTGGTT GGGCCTCTGG 
TGTATGGACT TGTACATGAT GAACTGTGAA CTTCTAGCCA CGTGTAGCGC CCTTGGGTAC 
TTGGAAGGAG GGACTTACCA CAAGGAGCCG GATTGCCTGG AGAGTGTGAA GGATTTGATC 
CGATACCTGA GGCACGAGGA TGAGACCCGA GATGTGCGGC AGCAGCTGGG AGCTGCACAG 
ATCCTGCAGA GCGACCTCCT GCCAATCCTC ACGCAGCATC GCCAGGACAA GCCTCTCTTC 
GATGCCGTGA TCAGGCTGAT GGTAAATTTG ACACAGCCAG CCTTGCTCTG TTTTGGCAGC 
GTGCCTAAGG ACTCCAGTGT ACGGCACCAT TTTCTGCAGG TTCTAACGTA CCTGCAAGCC 
TACAAAGAGG CCTTTGCCAG TGAGAAGGCA TTTGGAGTCC TCAGCGAGAC CTTGTATGAA 
TTGCTACAGC TGGGCTGGGA GGATCGGCAA GAAGAAGACA ACTTGCTGAT CGAGCGGATC 
CTTCTGCTGG TCAGAAATAT TCTCCATGTC CCGGCCAACC TTGAGCAGGA GAAGAGTATC 
GATGATGATG CCAGCATCCA CGACCGTCTC CTTTGGGCAA TTCACCTCAG TGGCATGGAC 
GACTTGCTCC TCTTCCTGTC CAGCTCATCC GCCGAGCAGC AGTGGAGCCT CCATGTGCTG 
GAGATCATCT CCCTCATGTT CCGAGACCAG ACCCCTGAGC AGCTAGCGGG AGTAGGGCAG 
GGACGCTTGG CTCAGGAGCG AAGCACGGAT GTGGCAGAAT TGGAGGTGCT GCGCCAACGG 
GAGATGGCGG AGAAGAGAGC TCGGGCCCTC CAGCGAGGAA ACAGGCACTC TCGATTTGGG 
GGCTCCTACA TTGTCCAGGG GTTGAAATCT ATTGGGGAGA AGGATGTCGT CTTTCACAAA 
GGCCTTCACA ATCTCCAGAA CTACAGCTCA GATCTGGGAA AGCAGCCCAG GAGGGTGCCC 
AAGCGTCGTC AGGCTGCCCA GGAGCTGTCT GTCCATCGCC GCTCTGTCCT GAATGTGAGA 
CTCTTCCTCA GAGACTTCTG CTCTGAGTTC CTGGAGAACT GCTACAACCC GCTCATGGGC 
GCGGTCAAGG ATCATCTGCT TCGGGAGAGA GCGCAGCAGC ATGACGAGAC TTACTACATG 
TGGGCAATGG CTTTCTTCAT GGCCTTCAAC CGAGCTGCCA CCTTCCGCCC CGGCCTTGTT 
TCTGAGACCC TCAGTATCCG TACCTTTCAC TTTGTGGAGC AGAACCTCAC CAACTACTAC 
GAGATGATGC TGACAGACCG CAAGGAGGCC GCCTCCTGGG CGCGCAGGAT GCACCTGGCC 
CTGAAGGCCT ACCAGGAGCT GCTGGCCACG GTGAACGAGA TGGACATGTG CCCAGATGAG 
GCTGTTAGGG AGAGCAGTCG TATCATCAAA AACAACATTT TCTATATGAT GGAGTACCGA 
GAACTATTCC TGGCGCTCTT TCGAAAGTTT GATGAGAGAT ACCATCCACG CTCATTCCTT 
CGAGACCTGG TGGAAACCAC CCACCTCTTC CTCAAAATGT TGGAGCGCTT TTGCCGGAGC 
CGCGGGAACC TGATGGTGCA GAACAAAAGA AAAAAGAGGA AAAAGAAAAA GAAGGTTCAG 
GACCAGGGTG TTGCTTTCTC ACAAAGCCCC GGGGAGCTGG AGGCCATGTG GCCAGCCCTG 
GCAGAGCAGC TGCTGCAGTG TGCCCAGGAC CCTGAGCTCA GTGTGGACCC CGTCGTTCCC 
TTTGATGCGG CCTCAGAGGT GCCAGTGGAG GAGCAGCGGG TAGAAGCCAT GGTGAGGATC 
CAAGACTGCC TTACGGCTGG CCAGGCCCCG CAAGCCCTGG CCCTCCTGCG GTCTGCCCGG 
GAAGTGTGGC CTGAAGGAAA TGCGTTTGGC TCTCCAGTCA TTTCCCCAGG GGAAGAAATG 
CAGTTGCTGA AACAAATCCT CTCCACGCCC CTTCCCCGGC AGCAGGAGCC AGAAGAAGGA 
GATGCAGAGG AGGAAGAGGA AGAGGAGGAG GAAGAGGAGT TACAGGTGGT CCAGGTGTCA 
GAGAAGGAGT TTAACTTTCT GGAATACCTG AAACGCTTCG CATCCTCAAC CATCGTGCGG 
GCCTACGTGC TTCTCCTGCG GAGCTACAGG CAGAACAGTG CTCACACCAA CCACTGCATC 
GCCAAGATGC TGCACCGGCT GGCCCATGGC CTGGGGATGG AAGCCCTGCT TTTCCAGCTG 
TCCCTGTTCT GCCTCTTCAA TCGGCTGCTT AGTGACCCAG CTGCTGCGGC CTACAAAGAG 
CTAGTGACTT TTGCCAAATA CATCATTGGC AAGTTCTTTG CGTTGGCTGC CGTGAACCAG 
AAAGCGTTTG TGGAGCTGCT GTTCTGGAAG AACACCGCAG TGGTTCGGGA AATGACCCAG 
GGATATGGCT CCCTCGACAG TGGGTCTTCC AGCCACAGAG CTCCTCTGTG GAGCCCTGAG 
GAAGAGGCCC AGCTTCAGGA ACTATACCTC GCCCACAAGG ATGTGGAAGG TCAAGATGTA 
GTGGAAACCA TATTGGCGCA CCTGAAAGTC GTTCCTCGAA CACGCAAGCA GGTCATCCAC 
CACCTGGTCC GGATGGGCCT GGCCGACAGC GTCAAGGAGT TCCAGAAGAG GAAAGGGACC 
CAGATTGTCT TGTGGACGGA GGACCAGGAG CTGGAGTTAC AGCGGCTCTT TGAGGAGTTC 
CGGGACTCTG ATGATGTTCT TGGTCAAATC ATGAAGAATA TCACAGCCAA ACGTTCACGG 
GCTCGAGTAG TGGACAAACT GTTGGCCCTG GGGTTGGTGT CTGAGCGGAG GCAGCTATAC 
AAGAAACGGA GAAAGAAGCT GGCGCCTTCT TGCATGAATG GAGAAAAGTC CCCGAGAGAC 
CCCTGGCAGG AAGATCCGGA AGAGGAAGAC GAACACTTGC CAGAGGACGA AAGTGAAGAT 
GAGGAGAGTG AGGAAGGCTT GCCATCAGGA CAGGGTCAGG GCAGCTCATC TCTCTCTGCT 
GAAAACCTCG GTGAGAGCCT TCGTCAGGAA GGCCTCTCTG CTCCCCTCCT GTGGCTCCAG 
AGCTCCCTGA TCCGAGCAGC AAATGACCGA GAAGAGGATG GCTGCTCCCA GGCAATCCCT 
CTGGTGCCTC TGACAGAGGA AAATGAGGAA GCAATGGAGA ACGAACAGTT TCAGCATCTG 
CTACGCAAGC TAGGGATCCG GCCGCCCAGC TCAGGGCAGG AAACCTTCTG GAGAATTCCA 
GCCAAACTGA GCTCCACCCA GCTTCGGAGG GTGGCTGCTT CTTTGAGTCA GCAAGAAAAC 
GAGGAGGAAA GGGAAGAGGA GCCAGAGCCA GGAGTCCCCG GAGAGCAGGG TCCCAGTGAG 
GAGCACCGGA CAGAAGCCCT GAGAGCCCTT CTGTCAGCCC GTAAGAGGAA AGCAGGCCTG 
GGGCCTACAG AAGAGGAGGC CACTGGGGAG GAAGAATGGA ACTCAGCGCC CAAGAAGCGG 
CAACTGCTGG ACAGCGACGA AGAGGAAGAT GATGAGGGGA GGAGGCAAGT GTCGGGAACG 
CCAAGAGTCC ACAGGAAGAA ACGGTTTCAG ATTGAGGATG AGGATGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Timeless ( NM_001164081.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001157553.1 Mus musculus timeless circadian clock 1 (Timeless), transcript variant 4, mRNA.

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CloneID	OMu16520
Clone ID Related Accession (Same CDS sequence)	XM_006513583.3 , NM_001164081.1 , NM_011589.2
Accession Version	NM_001164081.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-16
Organism	Mus musculus(house mouse)
Product	protein timeless homolog isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC052884.1, BC082770.1 and CJ088470.1. Transcript Variant: This variant (4) represents the longest transcript and encodes the longest isoform (2). Both variants 2 and 4 encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF071506.1, AB019001.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849383, SAMN00849388 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACTTGT ACATGATGAA CTGTGAACTT CTAGCCACGT GTAGCGCCCT TGGGTACTTG 
GAAGGAGGGA CTTACCACAA GGAGCCGGAT TGCCTGGAGA GTGTGAAGGA TTTGATCCGA 
TACCTGAGGC ACGAGGATGA GACCCGAGAT GTGCGGCAGC AGCTGGGAGC TGCACAGATC 
CTGCAGAGCG ACCTCCTGCC AATCCTCACG CAGCATCGCC AGGACAAGCC TCTCTTCGAT 
GCCGTGATCA GGCTGATGGT AAATTTGACA CAGCCAGCCT TGCTCTGTTT TGGCAGCGTG 
CCTAAGGACT CCAGTGTACG GCACCATTTT CTGCAGGTTC TAACGTACCT GCAAGCCTAC 
AAAGAGGCCT TTGCCAGTGA GAAGGCATTT GGAGTCCTCA GCGAGACCTT GTATGAATTG 
CTACAGCTGG GCTGGGAGGA TCGGCAAGAA GAAGACAACT TGCTGATCGA GCGGATCCTT 
CTGCTGGTCA GAAATATTCT CCATGTCCCG GCCAACCTTG AGCAGGAGAA GAGTATCGAT 
GATGATGCCA GCATCCACGA CCGTCTCCTT TGGGCAATTC ACCTCAGTGG CATGGACGAC 
TTGCTCCTCT TCCTGTCCAG CTCATCCGCC GAGCAGCAGT GGAGCCTCCA TGTGCTGGAG 
ATCATCTCCC TCATGTTCCG AGACCAGACC CCTGAGCAGC TAGCGGGAGT AGGGCAGGGA 
CGCTTGGCTC AGGAGCGAAG CACGGATGTG GCAGAATTGG AGGTGCTGCG CCAACGGGAG 
ATGGCGGAGA AGAGAGCTCG GGCCCTCCAG CGAGGAAACA GGCACTCTCG ATTTGGGGGC 
TCCTACATTG TCCAGGGGTT GAAATCTATT GGGGAGAAGG ATGTCGTCTT TCACAAAGGC 
CTTCACAATC TCCAGAACTA CAGCTCAGAT CTGGGAAAGC AGCCCAGGAG GGTGCCCAAG 
CGTCGTCAGG CTGCCCAGGA GCTGTCTGTC CATCGCCGCT CTGTCCTGAA TGTGAGACTC 
TTCCTCAGAG ACTTCTGCTC TGAGTTCCTG GAGAACTGCT ACAACCCGCT CATGGGCGCG 
GTCAAGGATC ATCTGCTTCG GGAGAGAGCG CAGCAGCATG ACGAGACTTA CTACATGTGG 
GCAATGGCTT TCTTCATGGC CTTCAACCGA GCTGCCACCT TCCGCCCCGG CCTTGTTTCT 
GAGACCCTCA GTATCCGTAC CTTTCACTTT GTGGAGCAGA ACCTCACCAA CTACTACGAG 
ATGATGCTGA CAGACCGCAA GGAGGCCGCC TCCTGGGCGC GCAGGATGCA CCTGGCCCTG 
AAGGCCTACC AGGAGCTGCT GGCCACGGTG AACGAGATGG ACATGTGCCC AGATGAGGCT 
GTTAGGGAGA GCAGTCGTAT CATCAAAAAC AACATTTTCT ATATGATGGA GTACCGAGAA 
CTATTCCTGG CGCTCTTTCG AAAGTTTGAT GAGAGATACC ATCCACGCTC ATTCCTTCGA 
GACCTGGTGG AAACCACCCA CCTCTTCCTC AAAATGTTGG AGCGCTTTTG CCGGAGCCGC 
GGGAACCTGA TGGTGCAGAA CAAAAGAAAA AAGAGGAAAA AGAAAAAGAA GGTTCAGGAC 
CAGGGTGTTG CTTTCTCACA AAGCCCCGGG GAGCTGGAGG CCATGTGGCC AGCCCTGGCA 
GAGCAGCTGC TGCAGTGTGC CCAGGACCCT GAGCTCAGTG TGGACCCCGT CGTTCCCTTT 
GATGCGGCCT CAGAGGTGCC AGTGGAGGAG CAGCGGGTAG AAGCCATGGT GAGGATCCAA 
GACTGCCTTA CGGCTGGCCA GGCCCCGCAA GCCCTGGCCC TCCTGCGGTC TGCCCGGGAA 
GTGTGGCCTG AAGGAAATGC GTTTGGCTCT CCAGTCATTT CCCCAGGGGA AGAAATGCAG 
TTGCTGAAAC AAATCCTCTC CACGCCCCTT CCCCGGCAGC AGGAGCCAGA AGAAGGAGAT 
GCAGAGGAGG AAGAGGAAGA GGAGGAGGAA GAGGAGTTAC AGGTGGTCCA GGTGTCAGAG 
AAGGAGTTTA ACTTTCTGGA ATACCTGAAA CGCTTCGCAT CCTCAACCAT CGTGCGGGCC 
TACGTGCTTC TCCTGCGGAG CTACAGGCAG AACAGTGCTC ACACCAACCA CTGCATCGCC 
AAGATGCTGC ACCGGCTGGC CCATGGCCTG GGGATGGAAG CCCTGCTTTT CCAGCTGTCC 
CTGTTCTGCC TCTTCAATCG GCTGCTTAGT GACCCAGCTG CTGCGGCCTA CAAAGAGCTA 
GTGACTTTTG CCAAATACAT CATTGGCAAG TTCTTTGCGT TGGCTGCCGT GAACCAGAAA 
GCGTTTGTGG AGCTGCTGTT CTGGAAGAAC ACCGCAGTGG TTCGGGAAAT GACCCAGGGA 
TATGGCTCCC TCGACAGTGG GTCTTCCAGC CACAGAGCTC CTCTGTGGAG CCCTGAGGAA 
GAGGCCCAGC TTCAGGAACT ATACCTCGCC CACAAGGATG TGGAAGGTCA AGATGTAGTG 
GAAACCATAT TGGCGCACCT GAAAGTCGTT CCTCGAACAC GCAAGCAGGT CATCCACCAC 
CTGGTCCGGA TGGGCCTGGC CGACAGCGTC AAGGAGTTCC AGAAGAGGAA AGGGACCCAG 
ATTGTCTTGT GGACGGAGGA CCAGGAGCTG GAGTTACAGC GGCTCTTTGA GGAGTTCCGG 
GACTCTGATG ATGTTCTTGG TCAAATCATG AAGAATATCA CAGCCAAACG TTCACGGGCT 
CGAGTAGTGG ACAAACTGTT GGCCCTGGGG TTGGTGTCTG AGCGGAGGCA GCTATACAAG 
AAACGGAGAA AGAAGCTGGC GCCTTCTTGC ATGCAGAATG GAGAAAAGTC CCCGAGAGAC 
CCCTGGCAGG AAGATCCGGA AGAGGAAGAC GAACACTTGC CAGAGGACGA AAGTGAAGAT 
GAGGAGAGTG AGGAAGGCTT GCCATCAGGA CAGGGTCAGG GCAGCTCATC TCTCTCTGCT 
GAAAACCTCG GTGAGAGCCT TCGTCAGGAA GGCCTCTCTG CTCCCCTCCT GTGGCTCCAG 
AGCTCCCTGA TCCGAGCAGC AAATGACCGA GAAGAGGATG GCTGCTCCCA GGCAATCCCT 
CTGGTGCCTC TGACAGAGGA AAATGAGGAA GCAATGGAGA ACGAACAGTT TCAGCATCTG 
CTACGCAAGC TAGGGATCCG GCCGCCCAGC TCAGGGCAGG AAACCTTCTG GAGAATTCCA 
GCCAAACTGA GCTCCACCCA GCTTCGGAGG GTGGCTGCTT CTTTGAGTCA GCAAGAAAAC 
GAGGAGGAAA GGGAAGAGGA GCCAGAGCCA GGAGTCCCCG GAGAGCAGGG TCCCAGTGAG 
GAGCACCGGA CAGAAGCCCT GAGAGCCCTT CTGTCAGCCC GTAAGAGGAA AGCAGGCCTG 
GGGCCTACAG AAGAGGAGGC CACTGGGGAG GAAGAATGGA ACTCAGCGCC CAAGAAGCGG 
CAACTGCTGG ACAGCGACGA AGAGGAAGAT GATGAGGGGA GGAGGCAAGC AGTGTCGGGA 
ACGCCAAGAG TCCACAGGAA GAAACGGTTT CAGATTGAGG ATGAGGATGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001157553.1
CDS	181..3774
Translation	MDLYMMNCELLATCSALGYLEGGTYHKEPDCLESVKDLIRYLRHEDETRDVRQQLGAAQILQSDLLPILTQHRQDKPLFDAVIRLMVNLTQPALLCFGSVPKDSSVRHHFLQVLTYLQAYKEAFASEKAFGVLSETLYELLQLGWEDRQEEDNLLIERILLLVRNILHVPANLEQEKSIDDDASIHDRLLWAIHLSGMDDLLLFLSSSSAEQQWSLHVLEIISLMFRDQTPEQLAGVGQGRLAQERSTDVAELEVLRQREMAEKRARALQRGNRHSRFGGSYIVQGLKSIGEKDVVFHKGLHNLQNYSSDLGKQPRRVPKRRQAAQELSVHRRSVLNVRLFLRDFCSEFLENCYNPLMGAVKDHLLRERAQQHDETYYMWAMAFFMAFNRAATFRPGLVSETLSIRTFHFVEQNLTNYYEMMLTDRKEAASWARRMHLALKAYQELLATVNEMDMCPDEAVRESSRIIKNNIFYMMEYRELFLALFRKFDERYHPRSFLRDLVETTHLFLKMLERFCRSRGNLMVQNKRKKRKKKKKVQDQGVAFSQSPGELEAMWPALAEQLLQCAQDPELSVDPVVPFDAASEVPVEEQRVEAMVRIQDCLTAGQAPQALALLRSAREVWPEGNAFGSPVISPGEEMQLLKQILSTPLPRQQEPEEGDAEEEEEEEEEEELQVVQVSEKEFNFLEYLKRFASSTIVRAYVLLLRSYRQNSAHTNHCIAKMLHRLAHGLGMEALLFQLSLFCLFNRLLSDPAAAAYKELVTFAKYIIGKFFALAAVNQKAFVELLFWKNTAVVREMTQGYGSLDSGSSSHRAPLWSPEEEAQLQELYLAHKDVEGQDVVETILAHLKVVPRTRKQVIHHLVRMGLADSVKEFQKRKGTQIVLWTEDQELELQRLFEEFRDSDDVLGQIMKNITAKRSRARVVDKLLALGLVSERRQLYKKRRKKLAPSCMQNGEKSPRDPWQEDPEEEDEHLPEDESEDEESEEGLPSGQGQGSSSLSAENLGESLRQEGLSAPLLWLQSSLIRAANDREEDGCSQAIPLVPLTEENEEAMENEQFQHLLRKLGIRPPSSGQETFWRIPAKLSSTQLRRVAASLSQQENEEEREEEPEPGVPGEQGPSEEHRTEALRALLSARKRKAGLGPTEEEATGEEEWNSAPKKRQLLDSDEEEDDEGRRQAVSGTPRVHRKKRFQIEDEDD

Target ORF information:

RefSeq Version	NM_001164081.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus timeless circadian clock 1 (Timeless), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001164081.1

ORF Insert Sequence:

ATGGACTTGT ACATGATGAA CTGTGAACTT CTAGCCACGT GTAGCGCCCT TGGGTACTTG 
GAAGGAGGGA CTTACCACAA GGAGCCGGAT TGCCTGGAGA GTGTGAAGGA TTTGATCCGA 
TACCTGAGGC ACGAGGATGA GACCCGAGAT GTGCGGCAGC AGCTGGGAGC TGCACAGATC 
CTGCAGAGCG ACCTCCTGCC AATCCTCACG CAGCATCGCC AGGACAAGCC TCTCTTCGAT 
GCCGTGATCA GGCTGATGGT AAATTTGACA CAGCCAGCCT TGCTCTGTTT TGGCAGCGTG 
CCTAAGGACT CCAGTGTACG GCACCATTTT CTGCAGGTTC TAACGTACCT GCAAGCCTAC 
AAAGAGGCCT TTGCCAGTGA GAAGGCATTT GGAGTCCTCA GCGAGACCTT GTATGAATTG 
CTACAGCTGG GCTGGGAGGA TCGGCAAGAA GAAGACAACT TGCTGATCGA GCGGATCCTT 
CTGCTGGTCA GAAATATTCT CCATGTCCCG GCCAACCTTG AGCAGGAGAA GAGTATCGAT 
GATGATGCCA GCATCCACGA CCGTCTCCTT TGGGCAATTC ACCTCAGTGG CATGGACGAC 
TTGCTCCTCT TCCTGTCCAG CTCATCCGCC GAGCAGCAGT GGAGCCTCCA TGTGCTGGAG 
ATCATCTCCC TCATGTTCCG AGACCAGACC CCTGAGCAGC TAGCGGGAGT AGGGCAGGGA 
CGCTTGGCTC AGGAGCGAAG CACGGATGTG GCAGAATTGG AGGTGCTGCG CCAACGGGAG 
ATGGCGGAGA AGAGAGCTCG GGCCCTCCAG CGAGGAAACA GGCACTCTCG ATTTGGGGGC 
TCCTACATTG TCCAGGGGTT GAAATCTATT GGGGAGAAGG ATGTCGTCTT TCACAAAGGC 
CTTCACAATC TCCAGAACTA CAGCTCAGAT CTGGGAAAGC AGCCCAGGAG GGTGCCCAAG 
CGTCGTCAGG CTGCCCAGGA GCTGTCTGTC CATCGCCGCT CTGTCCTGAA TGTGAGACTC 
TTCCTCAGAG ACTTCTGCTC TGAGTTCCTG GAGAACTGCT ACAACCCGCT CATGGGCGCG 
GTCAAGGATC ATCTGCTTCG GGAGAGAGCG CAGCAGCATG ACGAGACTTA CTACATGTGG 
GCAATGGCTT TCTTCATGGC CTTCAACCGA GCTGCCACCT TCCGCCCCGG CCTTGTTTCT 
GAGACCCTCA GTATCCGTAC CTTTCACTTT GTGGAGCAGA ACCTCACCAA CTACTACGAG 
ATGATGCTGA CAGACCGCAA GGAGGCCGCC TCCTGGGCGC GCAGGATGCA CCTGGCCCTG 
AAGGCCTACC AGGAGCTGCT GGCCACGGTG AACGAGATGG ACATGTGCCC AGATGAGGCT 
GTTAGGGAGA GCAGTCGTAT CATCAAAAAC AACATTTTCT ATATGATGGA GTACCGAGAA 
CTATTCCTGG CGCTCTTTCG AAAGTTTGAT GAGAGATACC ATCCACGCTC ATTCCTTCGA 
GACCTGGTGG AAACCACCCA CCTCTTCCTC AAAATGTTGG AGCGCTTTTG CCGGAGCCGC 
GGGAACCTGA TGGTGCAGAA CAAAAGAAAA AAGAGGAAAA AGAAAAAGAA GGTTCAGGAC 
CAGGGTGTTG CTTTCTCACA AAGCCCCGGG GAGCTGGAGG CCATGTGGCC AGCCCTGGCA 
GAGCAGCTGC TGCAGTGTGC CCAGGACCCT GAGCTCAGTG TGGACCCCGT CGTTCCCTTT 
GATGCGGCCT CAGAGGTGCC AGTGGAGGAG CAGCGGGTAG AAGCCATGGT GAGGATCCAA 
GACTGCCTTA CGGCTGGCCA GGCCCCGCAA GCCCTGGCCC TCCTGCGGTC TGCCCGGGAA 
GTGTGGCCTG AAGGAAATGC GTTTGGCTCT CCAGTCATTT CCCCAGGGGA AGAAATGCAG 
TTGCTGAAAC AAATCCTCTC CACGCCCCTT CCCCGGCAGC AGGAGCCAGA AGAAGGAGAT 
GCAGAGGAGG AAGAGGAAGA GGAGGAGGAA GAGGAGTTAC AGGTGGTCCA GGTGTCAGAG 
AAGGAGTTTA ACTTTCTGGA ATACCTGAAA CGCTTCGCAT CCTCAACCAT CGTGCGGGCC 
TACGTGCTTC TCCTGCGGAG CTACAGGCAG AACAGTGCTC ACACCAACCA CTGCATCGCC 
AAGATGCTGC ACCGGCTGGC CCATGGCCTG GGGATGGAAG CCCTGCTTTT CCAGCTGTCC 
CTGTTCTGCC TCTTCAATCG GCTGCTTAGT GACCCAGCTG CTGCGGCCTA CAAAGAGCTA 
GTGACTTTTG CCAAATACAT CATTGGCAAG TTCTTTGCGT TGGCTGCCGT GAACCAGAAA 
GCGTTTGTGG AGCTGCTGTT CTGGAAGAAC ACCGCAGTGG TTCGGGAAAT GACCCAGGGA 
TATGGCTCCC TCGACAGTGG GTCTTCCAGC CACAGAGCTC CTCTGTGGAG CCCTGAGGAA 
GAGGCCCAGC TTCAGGAACT ATACCTCGCC CACAAGGATG TGGAAGGTCA AGATGTAGTG 
GAAACCATAT TGGCGCACCT GAAAGTCGTT CCTCGAACAC GCAAGCAGGT CATCCACCAC 
CTGGTCCGGA TGGGCCTGGC CGACAGCGTC AAGGAGTTCC AGAAGAGGAA AGGGACCCAG 
ATTGTCTTGT GGACGGAGGA CCAGGAGCTG GAGTTACAGC GGCTCTTTGA GGAGTTCCGG 
GACTCTGATG ATGTTCTTGG TCAAATCATG AAGAATATCA CAGCCAAACG TTCACGGGCT 
CGAGTAGTGG ACAAACTGTT GGCCCTGGGG TTGGTGTCTG AGCGGAGGCA GCTATACAAG 
AAACGGAGAA AGAAGCTGGC GCCTTCTTGC ATGCAGAATG GAGAAAAGTC CCCGAGAGAC 
CCCTGGCAGG AAGATCCGGA AGAGGAAGAC GAACACTTGC CAGAGGACGA AAGTGAAGAT 
GAGGAGAGTG AGGAAGGCTT GCCATCAGGA CAGGGTCAGG GCAGCTCATC TCTCTCTGCT 
GAAAACCTCG GTGAGAGCCT TCGTCAGGAA GGCCTCTCTG CTCCCCTCCT GTGGCTCCAG 
AGCTCCCTGA TCCGAGCAGC AAATGACCGA GAAGAGGATG GCTGCTCCCA GGCAATCCCT 
CTGGTGCCTC TGACAGAGGA AAATGAGGAA GCAATGGAGA ACGAACAGTT TCAGCATCTG 
CTACGCAAGC TAGGGATCCG GCCGCCCAGC TCAGGGCAGG AAACCTTCTG GAGAATTCCA 
GCCAAACTGA GCTCCACCCA GCTTCGGAGG GTGGCTGCTT CTTTGAGTCA GCAAGAAAAC 
GAGGAGGAAA GGGAAGAGGA GCCAGAGCCA GGAGTCCCCG GAGAGCAGGG TCCCAGTGAG 
GAGCACCGGA CAGAAGCCCT GAGAGCCCTT CTGTCAGCCC GTAAGAGGAA AGCAGGCCTG 
GGGCCTACAG AAGAGGAGGC CACTGGGGAG GAAGAATGGA ACTCAGCGCC CAAGAAGCGG 
CAACTGCTGG ACAGCGACGA AGAGGAAGAT GATGAGGGGA GGAGGCAAGC AGTGTCGGGA 
ACGCCAAGAG TCCACAGGAA GAAACGGTTT CAGATTGAGG ATGAGGATGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Timeless ( NM_011589.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035719.1 Mus musculus timeless circadian clock 1 (Timeless), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu16520
Clone ID Related Accession (Same CDS sequence)	XM_006513583.3 , NM_001164081.1 , NM_011589.2
Accession Version	NM_011589.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-16
Organism	Mus musculus(house mouse)
Product	protein timeless homolog isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF098161.1 and BC082770.1. On Aug 12, 2009 this sequence version replaced NM_011589.1. Transcript Variant: This variant (2) represents use of an alternate promoter and 5' UTR, compared to variant 4. Both variants 2 and 4 encode the same isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF098161.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACTTGT ACATGATGAA CTGTGAACTT CTAGCCACGT GTAGCGCCCT TGGGTACTTG 
GAAGGAGGGA CTTACCACAA GGAGCCGGAT TGCCTGGAGA GTGTGAAGGA TTTGATCCGA 
TACCTGAGGC ACGAGGATGA GACCCGAGAT GTGCGGCAGC AGCTGGGAGC TGCACAGATC 
CTGCAGAGCG ACCTCCTGCC AATCCTCACG CAGCATCGCC AGGACAAGCC TCTCTTCGAT 
GCCGTGATCA GGCTGATGGT AAATTTGACA CAGCCAGCCT TGCTCTGTTT TGGCAGCGTG 
CCTAAGGACT CCAGTGTACG GCACCATTTT CTGCAGGTTC TAACGTACCT GCAAGCCTAC 
AAAGAGGCCT TTGCCAGTGA GAAGGCATTT GGAGTCCTCA GCGAGACCTT GTATGAATTG 
CTACAGCTGG GCTGGGAGGA TCGGCAAGAA GAAGACAACT TGCTGATCGA GCGGATCCTT 
CTGCTGGTCA GAAATATTCT CCATGTCCCG GCCAACCTTG AGCAGGAGAA GAGTATCGAT 
GATGATGCCA GCATCCACGA CCGTCTCCTT TGGGCAATTC ACCTCAGTGG CATGGACGAC 
TTGCTCCTCT TCCTGTCCAG CTCATCCGCC GAGCAGCAGT GGAGCCTCCA TGTGCTGGAG 
ATCATCTCCC TCATGTTCCG AGACCAGACC CCTGAGCAGC TAGCGGGAGT AGGGCAGGGA 
CGCTTGGCTC AGGAGCGAAG CACGGATGTG GCAGAATTGG AGGTGCTGCG CCAACGGGAG 
ATGGCGGAGA AGAGAGCTCG GGCCCTCCAG CGAGGAAACA GGCACTCTCG ATTTGGGGGC 
TCCTACATTG TCCAGGGGTT GAAATCTATT GGGGAGAAGG ATGTCGTCTT TCACAAAGGC 
CTTCACAATC TCCAGAACTA CAGCTCAGAT CTGGGAAAGC AGCCCAGGAG GGTGCCCAAG 
CGTCGTCAGG CTGCCCAGGA GCTGTCTGTC CATCGCCGCT CTGTCCTGAA TGTGAGACTC 
TTCCTCAGAG ACTTCTGCTC TGAGTTCCTG GAGAACTGCT ACAACCCGCT CATGGGCGCG 
GTCAAGGATC ATCTGCTTCG GGAGAGAGCG CAGCAGCATG ACGAGACTTA CTACATGTGG 
GCAATGGCTT TCTTCATGGC CTTCAACCGA GCTGCCACCT TCCGCCCCGG CCTTGTTTCT 
GAGACCCTCA GTATCCGTAC CTTTCACTTT GTGGAGCAGA ACCTCACCAA CTACTACGAG 
ATGATGCTGA CAGACCGCAA GGAGGCCGCC TCCTGGGCGC GCAGGATGCA CCTGGCCCTG 
AAGGCCTACC AGGAGCTGCT GGCCACGGTG AACGAGATGG ACATGTGCCC AGATGAGGCT 
GTTAGGGAGA GCAGTCGTAT CATCAAAAAC AACATTTTCT ATATGATGGA GTACCGAGAA 
CTATTCCTGG CGCTCTTTCG AAAGTTTGAT GAGAGATACC ATCCACGCTC ATTCCTTCGA 
GACCTGGTGG AAACCACCCA CCTCTTCCTC AAAATGTTGG AGCGCTTTTG CCGGAGCCGC 
GGGAACCTGA TGGTGCAGAA CAAAAGAAAA AAGAGGAAAA AGAAAAAGAA GGTTCAGGAC 
CAGGGTGTTG CTTTCTCACA AAGCCCCGGG GAGCTGGAGG CCATGTGGCC AGCCCTGGCA 
GAGCAGCTGC TGCAGTGTGC CCAGGACCCT GAGCTCAGTG TGGACCCCGT CGTTCCCTTT 
GATGCGGCCT CAGAGGTGCC AGTGGAGGAG CAGCGGGTAG AAGCCATGGT GAGGATCCAA 
GACTGCCTTA CGGCTGGCCA GGCCCCGCAA GCCCTGGCCC TCCTGCGGTC TGCCCGGGAA 
GTGTGGCCTG AAGGAAATGC GTTTGGCTCT CCAGTCATTT CCCCAGGGGA AGAAATGCAG 
TTGCTGAAAC AAATCCTCTC CACGCCCCTT CCCCGGCAGC AGGAGCCAGA AGAAGGAGAT 
GCAGAGGAGG AAGAGGAAGA GGAGGAGGAA GAGGAGTTAC AGGTGGTCCA GGTGTCAGAG 
AAGGAGTTTA ACTTTCTGGA ATACCTGAAA CGCTTCGCAT CCTCAACCAT CGTGCGGGCC 
TACGTGCTTC TCCTGCGGAG CTACAGGCAG AACAGTGCTC ACACCAACCA CTGCATCGCC 
AAGATGCTGC ACCGGCTGGC CCATGGCCTG GGGATGGAAG CCCTGCTTTT CCAGCTGTCC 
CTGTTCTGCC TCTTCAATCG GCTGCTTAGT GACCCAGCTG CTGCGGCCTA CAAAGAGCTA 
GTGACTTTTG CCAAATACAT CATTGGCAAG TTCTTTGCGT TGGCTGCCGT GAACCAGAAA 
GCGTTTGTGG AGCTGCTGTT CTGGAAGAAC ACCGCAGTGG TTCGGGAAAT GACCCAGGGA 
TATGGCTCCC TCGACAGTGG GTCTTCCAGC CACAGAGCTC CTCTGTGGAG CCCTGAGGAA 
GAGGCCCAGC TTCAGGAACT ATACCTCGCC CACAAGGATG TGGAAGGTCA AGATGTAGTG 
GAAACCATAT TGGCGCACCT GAAAGTCGTT CCTCGAACAC GCAAGCAGGT CATCCACCAC 
CTGGTCCGGA TGGGCCTGGC CGACAGCGTC AAGGAGTTCC AGAAGAGGAA AGGGACCCAG 
ATTGTCTTGT GGACGGAGGA CCAGGAGCTG GAGTTACAGC GGCTCTTTGA GGAGTTCCGG 
GACTCTGATG ATGTTCTTGG TCAAATCATG AAGAATATCA CAGCCAAACG TTCACGGGCT 
CGAGTAGTGG ACAAACTGTT GGCCCTGGGG TTGGTGTCTG AGCGGAGGCA GCTATACAAG 
AAACGGAGAA AGAAGCTGGC GCCTTCTTGC ATGCAGAATG GAGAAAAGTC CCCGAGAGAC 
CCCTGGCAGG AAGATCCGGA AGAGGAAGAC GAACACTTGC CAGAGGACGA AAGTGAAGAT 
GAGGAGAGTG AGGAAGGCTT GCCATCAGGA CAGGGTCAGG GCAGCTCATC TCTCTCTGCT 
GAAAACCTCG GTGAGAGCCT TCGTCAGGAA GGCCTCTCTG CTCCCCTCCT GTGGCTCCAG 
AGCTCCCTGA TCCGAGCAGC AAATGACCGA GAAGAGGATG GCTGCTCCCA GGCAATCCCT 
CTGGTGCCTC TGACAGAGGA AAATGAGGAA GCAATGGAGA ACGAACAGTT TCAGCATCTG 
CTACGCAAGC TAGGGATCCG GCCGCCCAGC TCAGGGCAGG AAACCTTCTG GAGAATTCCA 
GCCAAACTGA GCTCCACCCA GCTTCGGAGG GTGGCTGCTT CTTTGAGTCA GCAAGAAAAC 
GAGGAGGAAA GGGAAGAGGA GCCAGAGCCA GGAGTCCCCG GAGAGCAGGG TCCCAGTGAG 
GAGCACCGGA CAGAAGCCCT GAGAGCCCTT CTGTCAGCCC GTAAGAGGAA AGCAGGCCTG 
GGGCCTACAG AAGAGGAGGC CACTGGGGAG GAAGAATGGA ACTCAGCGCC CAAGAAGCGG 
CAACTGCTGG ACAGCGACGA AGAGGAAGAT GATGAGGGGA GGAGGCAAGC AGTGTCGGGA 
ACGCCAAGAG TCCACAGGAA GAAACGGTTT CAGATTGAGG ATGAGGATGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035719.1
CDS	124..3717
Translation	MDLYMMNCELLATCSALGYLEGGTYHKEPDCLESVKDLIRYLRHEDETRDVRQQLGAAQILQSDLLPILTQHRQDKPLFDAVIRLMVNLTQPALLCFGSVPKDSSVRHHFLQVLTYLQAYKEAFASEKAFGVLSETLYELLQLGWEDRQEEDNLLIERILLLVRNILHVPANLEQEKSIDDDASIHDRLLWAIHLSGMDDLLLFLSSSSAEQQWSLHVLEIISLMFRDQTPEQLAGVGQGRLAQERSTDVAELEVLRQREMAEKRARALQRGNRHSRFGGSYIVQGLKSIGEKDVVFHKGLHNLQNYSSDLGKQPRRVPKRRQAAQELSVHRRSVLNVRLFLRDFCSEFLENCYNPLMGAVKDHLLRERAQQHDETYYMWAMAFFMAFNRAATFRPGLVSETLSIRTFHFVEQNLTNYYEMMLTDRKEAASWARRMHLALKAYQELLATVNEMDMCPDEAVRESSRIIKNNIFYMMEYRELFLALFRKFDERYHPRSFLRDLVETTHLFLKMLERFCRSRGNLMVQNKRKKRKKKKKVQDQGVAFSQSPGELEAMWPALAEQLLQCAQDPELSVDPVVPFDAASEVPVEEQRVEAMVRIQDCLTAGQAPQALALLRSAREVWPEGNAFGSPVISPGEEMQLLKQILSTPLPRQQEPEEGDAEEEEEEEEEEELQVVQVSEKEFNFLEYLKRFASSTIVRAYVLLLRSYRQNSAHTNHCIAKMLHRLAHGLGMEALLFQLSLFCLFNRLLSDPAAAAYKELVTFAKYIIGKFFALAAVNQKAFVELLFWKNTAVVREMTQGYGSLDSGSSSHRAPLWSPEEEAQLQELYLAHKDVEGQDVVETILAHLKVVPRTRKQVIHHLVRMGLADSVKEFQKRKGTQIVLWTEDQELELQRLFEEFRDSDDVLGQIMKNITAKRSRARVVDKLLALGLVSERRQLYKKRRKKLAPSCMQNGEKSPRDPWQEDPEEEDEHLPEDESEDEESEEGLPSGQGQGSSSLSAENLGESLRQEGLSAPLLWLQSSLIRAANDREEDGCSQAIPLVPLTEENEEAMENEQFQHLLRKLGIRPPSSGQETFWRIPAKLSSTQLRRVAASLSQQENEEEREEEPEPGVPGEQGPSEEHRTEALRALLSARKRKAGLGPTEEEATGEEEWNSAPKKRQLLDSDEEEDDEGRRQAVSGTPRVHRKKRFQIEDEDD

Target ORF information:

RefSeq Version	NM_011589.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus timeless circadian clock 1 (Timeless), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011589.2

ORF Insert Sequence:

ATGGACTTGT ACATGATGAA CTGTGAACTT CTAGCCACGT GTAGCGCCCT TGGGTACTTG 
GAAGGAGGGA CTTACCACAA GGAGCCGGAT TGCCTGGAGA GTGTGAAGGA TTTGATCCGA 
TACCTGAGGC ACGAGGATGA GACCCGAGAT GTGCGGCAGC AGCTGGGAGC TGCACAGATC 
CTGCAGAGCG ACCTCCTGCC AATCCTCACG CAGCATCGCC AGGACAAGCC TCTCTTCGAT 
GCCGTGATCA GGCTGATGGT AAATTTGACA CAGCCAGCCT TGCTCTGTTT TGGCAGCGTG 
CCTAAGGACT CCAGTGTACG GCACCATTTT CTGCAGGTTC TAACGTACCT GCAAGCCTAC 
AAAGAGGCCT TTGCCAGTGA GAAGGCATTT GGAGTCCTCA GCGAGACCTT GTATGAATTG 
CTACAGCTGG GCTGGGAGGA TCGGCAAGAA GAAGACAACT TGCTGATCGA GCGGATCCTT 
CTGCTGGTCA GAAATATTCT CCATGTCCCG GCCAACCTTG AGCAGGAGAA GAGTATCGAT 
GATGATGCCA GCATCCACGA CCGTCTCCTT TGGGCAATTC ACCTCAGTGG CATGGACGAC 
TTGCTCCTCT TCCTGTCCAG CTCATCCGCC GAGCAGCAGT GGAGCCTCCA TGTGCTGGAG 
ATCATCTCCC TCATGTTCCG AGACCAGACC CCTGAGCAGC TAGCGGGAGT AGGGCAGGGA 
CGCTTGGCTC AGGAGCGAAG CACGGATGTG GCAGAATTGG AGGTGCTGCG CCAACGGGAG 
ATGGCGGAGA AGAGAGCTCG GGCCCTCCAG CGAGGAAACA GGCACTCTCG ATTTGGGGGC 
TCCTACATTG TCCAGGGGTT GAAATCTATT GGGGAGAAGG ATGTCGTCTT TCACAAAGGC 
CTTCACAATC TCCAGAACTA CAGCTCAGAT CTGGGAAAGC AGCCCAGGAG GGTGCCCAAG 
CGTCGTCAGG CTGCCCAGGA GCTGTCTGTC CATCGCCGCT CTGTCCTGAA TGTGAGACTC 
TTCCTCAGAG ACTTCTGCTC TGAGTTCCTG GAGAACTGCT ACAACCCGCT CATGGGCGCG 
GTCAAGGATC ATCTGCTTCG GGAGAGAGCG CAGCAGCATG ACGAGACTTA CTACATGTGG 
GCAATGGCTT TCTTCATGGC CTTCAACCGA GCTGCCACCT TCCGCCCCGG CCTTGTTTCT 
GAGACCCTCA GTATCCGTAC CTTTCACTTT GTGGAGCAGA ACCTCACCAA CTACTACGAG 
ATGATGCTGA CAGACCGCAA GGAGGCCGCC TCCTGGGCGC GCAGGATGCA CCTGGCCCTG 
AAGGCCTACC AGGAGCTGCT GGCCACGGTG AACGAGATGG ACATGTGCCC AGATGAGGCT 
GTTAGGGAGA GCAGTCGTAT CATCAAAAAC AACATTTTCT ATATGATGGA GTACCGAGAA 
CTATTCCTGG CGCTCTTTCG AAAGTTTGAT GAGAGATACC ATCCACGCTC ATTCCTTCGA 
GACCTGGTGG AAACCACCCA CCTCTTCCTC AAAATGTTGG AGCGCTTTTG CCGGAGCCGC 
GGGAACCTGA TGGTGCAGAA CAAAAGAAAA AAGAGGAAAA AGAAAAAGAA GGTTCAGGAC 
CAGGGTGTTG CTTTCTCACA AAGCCCCGGG GAGCTGGAGG CCATGTGGCC AGCCCTGGCA 
GAGCAGCTGC TGCAGTGTGC CCAGGACCCT GAGCTCAGTG TGGACCCCGT CGTTCCCTTT 
GATGCGGCCT CAGAGGTGCC AGTGGAGGAG CAGCGGGTAG AAGCCATGGT GAGGATCCAA 
GACTGCCTTA CGGCTGGCCA GGCCCCGCAA GCCCTGGCCC TCCTGCGGTC TGCCCGGGAA 
GTGTGGCCTG AAGGAAATGC GTTTGGCTCT CCAGTCATTT CCCCAGGGGA AGAAATGCAG 
TTGCTGAAAC AAATCCTCTC CACGCCCCTT CCCCGGCAGC AGGAGCCAGA AGAAGGAGAT 
GCAGAGGAGG AAGAGGAAGA GGAGGAGGAA GAGGAGTTAC AGGTGGTCCA GGTGTCAGAG 
AAGGAGTTTA ACTTTCTGGA ATACCTGAAA CGCTTCGCAT CCTCAACCAT CGTGCGGGCC 
TACGTGCTTC TCCTGCGGAG CTACAGGCAG AACAGTGCTC ACACCAACCA CTGCATCGCC 
AAGATGCTGC ACCGGCTGGC CCATGGCCTG GGGATGGAAG CCCTGCTTTT CCAGCTGTCC 
CTGTTCTGCC TCTTCAATCG GCTGCTTAGT GACCCAGCTG CTGCGGCCTA CAAAGAGCTA 
GTGACTTTTG CCAAATACAT CATTGGCAAG TTCTTTGCGT TGGCTGCCGT GAACCAGAAA 
GCGTTTGTGG AGCTGCTGTT CTGGAAGAAC ACCGCAGTGG TTCGGGAAAT GACCCAGGGA 
TATGGCTCCC TCGACAGTGG GTCTTCCAGC CACAGAGCTC CTCTGTGGAG CCCTGAGGAA 
GAGGCCCAGC TTCAGGAACT ATACCTCGCC CACAAGGATG TGGAAGGTCA AGATGTAGTG 
GAAACCATAT TGGCGCACCT GAAAGTCGTT CCTCGAACAC GCAAGCAGGT CATCCACCAC 
CTGGTCCGGA TGGGCCTGGC CGACAGCGTC AAGGAGTTCC AGAAGAGGAA AGGGACCCAG 
ATTGTCTTGT GGACGGAGGA CCAGGAGCTG GAGTTACAGC GGCTCTTTGA GGAGTTCCGG 
GACTCTGATG ATGTTCTTGG TCAAATCATG AAGAATATCA CAGCCAAACG TTCACGGGCT 
CGAGTAGTGG ACAAACTGTT GGCCCTGGGG TTGGTGTCTG AGCGGAGGCA GCTATACAAG 
AAACGGAGAA AGAAGCTGGC GCCTTCTTGC ATGCAGAATG GAGAAAAGTC CCCGAGAGAC 
CCCTGGCAGG AAGATCCGGA AGAGGAAGAC GAACACTTGC CAGAGGACGA AAGTGAAGAT 
GAGGAGAGTG AGGAAGGCTT GCCATCAGGA CAGGGTCAGG GCAGCTCATC TCTCTCTGCT 
GAAAACCTCG GTGAGAGCCT TCGTCAGGAA GGCCTCTCTG CTCCCCTCCT GTGGCTCCAG 
AGCTCCCTGA TCCGAGCAGC AAATGACCGA GAAGAGGATG GCTGCTCCCA GGCAATCCCT 
CTGGTGCCTC TGACAGAGGA AAATGAGGAA GCAATGGAGA ACGAACAGTT TCAGCATCTG 
CTACGCAAGC TAGGGATCCG GCCGCCCAGC TCAGGGCAGG AAACCTTCTG GAGAATTCCA 
GCCAAACTGA GCTCCACCCA GCTTCGGAGG GTGGCTGCTT CTTTGAGTCA GCAAGAAAAC 
GAGGAGGAAA GGGAAGAGGA GCCAGAGCCA GGAGTCCCCG GAGAGCAGGG TCCCAGTGAG 
GAGCACCGGA CAGAAGCCCT GAGAGCCCTT CTGTCAGCCC GTAAGAGGAA AGCAGGCCTG 
GGGCCTACAG AAGAGGAGGC CACTGGGGAG GAAGAATGGA ACTCAGCGCC CAAGAAGCGG 
CAACTGCTGG ACAGCGACGA AGAGGAAGAT GATGAGGGGA GGAGGCAAGC AGTGTCGGGA 
ACGCCAAGAG TCCACAGGAA GAAACGGTTT CAGATTGAGG ATGAGGATGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Timeless ( NM_001136082.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001129554.1 Mus musculus timeless circadian clock 1 (Timeless), transcript variant 1, mRNA.

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CloneID	OMu16551
Clone ID Related Accession (Same CDS sequence)	NM_001136082.2
Accession Version	NM_001136082.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3591bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-16
Organism	Mus musculus(house mouse)
Product	protein timeless homolog isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC052884.1 and BC082770.1. On Aug 12, 2009 this sequence version replaced NM_001136082.1. Transcript Variant: This variant (1) uses an alternate in-frame splice site in the 3' coding region, compared to variant 4. This variant is distinct from but the same length as variant 3. The resulting isoform (1) lacks one aa near the C-terminus, compared to isoform 2, and is distinct from isoform 3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC082770.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849383, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACTTGT ACATGATGAA CTGTGAACTT CTAGCCACGT GTAGCGCCCT TGGGTACTTG 
GAAGGAGGGA CTTACCACAA GGAGCCGGAT TGCCTGGAGA GTGTGAAGGA TTTGATCCGA 
TACCTGAGGC ACGAGGATGA GACCCGAGAT GTGCGGCAGC AGCTGGGAGC TGCACAGATC 
CTGCAGAGCG ACCTCCTGCC AATCCTCACG CAGCATCGCC AGGACAAGCC TCTCTTCGAT 
GCCGTGATCA GGCTGATGGT AAATTTGACA CAGCCAGCCT TGCTCTGTTT TGGCAGCGTG 
CCTAAGGACT CCAGTGTACG GCACCATTTT CTGCAGGTTC TAACGTACCT GCAAGCCTAC 
AAAGAGGCCT TTGCCAGTGA GAAGGCATTT GGAGTCCTCA GCGAGACCTT GTATGAATTG 
CTACAGCTGG GCTGGGAGGA TCGGCAAGAA GAAGACAACT TGCTGATCGA GCGGATCCTT 
CTGCTGGTCA GAAATATTCT CCATGTCCCG GCCAACCTTG AGCAGGAGAA GAGTATCGAT 
GATGATGCCA GCATCCACGA CCGTCTCCTT TGGGCAATTC ACCTCAGTGG CATGGACGAC 
TTGCTCCTCT TCCTGTCCAG CTCATCCGCC GAGCAGCAGT GGAGCCTCCA TGTGCTGGAG 
ATCATCTCCC TCATGTTCCG AGACCAGACC CCTGAGCAGC TAGCGGGAGT AGGGCAGGGA 
CGCTTGGCTC AGGAGCGAAG CACGGATGTG GCAGAATTGG AGGTGCTGCG CCAACGGGAG 
ATGGCGGAGA AGAGAGCTCG GGCCCTCCAG CGAGGAAACA GGCACTCTCG ATTTGGGGGC 
TCCTACATTG TCCAGGGGTT GAAATCTATT GGGGAGAAGG ATGTCGTCTT TCACAAAGGC 
CTTCACAATC TCCAGAACTA CAGCTCAGAT CTGGGAAAGC AGCCCAGGAG GGTGCCCAAG 
CGTCGTCAGG CTGCCCAGGA GCTGTCTGTC CATCGCCGCT CTGTCCTGAA TGTGAGACTC 
TTCCTCAGAG ACTTCTGCTC TGAGTTCCTG GAGAACTGCT ACAACCCGCT CATGGGCGCG 
GTCAAGGATC ATCTGCTTCG GGAGAGAGCG CAGCAGCATG ACGAGACTTA CTACATGTGG 
GCAATGGCTT TCTTCATGGC CTTCAACCGA GCTGCCACCT TCCGCCCCGG CCTTGTTTCT 
GAGACCCTCA GTATCCGTAC CTTTCACTTT GTGGAGCAGA ACCTCACCAA CTACTACGAG 
ATGATGCTGA CAGACCGCAA GGAGGCCGCC TCCTGGGCGC GCAGGATGCA CCTGGCCCTG 
AAGGCCTACC AGGAGCTGCT GGCCACGGTG AACGAGATGG ACATGTGCCC AGATGAGGCT 
GTTAGGGAGA GCAGTCGTAT CATCAAAAAC AACATTTTCT ATATGATGGA GTACCGAGAA 
CTATTCCTGG CGCTCTTTCG AAAGTTTGAT GAGAGATACC ATCCACGCTC ATTCCTTCGA 
GACCTGGTGG AAACCACCCA CCTCTTCCTC AAAATGTTGG AGCGCTTTTG CCGGAGCCGC 
GGGAACCTGA TGGTGCAGAA CAAAAGAAAA AAGAGGAAAA AGAAAAAGAA GGTTCAGGAC 
CAGGGTGTTG CTTTCTCACA AAGCCCCGGG GAGCTGGAGG CCATGTGGCC AGCCCTGGCA 
GAGCAGCTGC TGCAGTGTGC CCAGGACCCT GAGCTCAGTG TGGACCCCGT CGTTCCCTTT 
GATGCGGCCT CAGAGGTGCC AGTGGAGGAG CAGCGGGTAG AAGCCATGGT GAGGATCCAA 
GACTGCCTTA CGGCTGGCCA GGCCCCGCAA GCCCTGGCCC TCCTGCGGTC TGCCCGGGAA 
GTGTGGCCTG AAGGAAATGC GTTTGGCTCT CCAGTCATTT CCCCAGGGGA AGAAATGCAG 
TTGCTGAAAC AAATCCTCTC CACGCCCCTT CCCCGGCAGC AGGAGCCAGA AGAAGGAGAT 
GCAGAGGAGG AAGAGGAAGA GGAGGAGGAA GAGGAGTTAC AGGTGGTCCA GGTGTCAGAG 
AAGGAGTTTA ACTTTCTGGA ATACCTGAAA CGCTTCGCAT CCTCAACCAT CGTGCGGGCC 
TACGTGCTTC TCCTGCGGAG CTACAGGCAG AACAGTGCTC ACACCAACCA CTGCATCGCC 
AAGATGCTGC ACCGGCTGGC CCATGGCCTG GGGATGGAAG CCCTGCTTTT CCAGCTGTCC 
CTGTTCTGCC TCTTCAATCG GCTGCTTAGT GACCCAGCTG CTGCGGCCTA CAAAGAGCTA 
GTGACTTTTG CCAAATACAT CATTGGCAAG TTCTTTGCGT TGGCTGCCGT GAACCAGAAA 
GCGTTTGTGG AGCTGCTGTT CTGGAAGAAC ACCGCAGTGG TTCGGGAAAT GACCCAGGGA 
TATGGCTCCC TCGACAGTGG GTCTTCCAGC CACAGAGCTC CTCTGTGGAG CCCTGAGGAA 
GAGGCCCAGC TTCAGGAACT ATACCTCGCC CACAAGGATG TGGAAGGTCA AGATGTAGTG 
GAAACCATAT TGGCGCACCT GAAAGTCGTT CCTCGAACAC GCAAGCAGGT CATCCACCAC 
CTGGTCCGGA TGGGCCTGGC CGACAGCGTC AAGGAGTTCC AGAAGAGGAA AGGGACCCAG 
ATTGTCTTGT GGACGGAGGA CCAGGAGCTG GAGTTACAGC GGCTCTTTGA GGAGTTCCGG 
GACTCTGATG ATGTTCTTGG TCAAATCATG AAGAATATCA CAGCCAAACG TTCACGGGCT 
CGAGTAGTGG ACAAACTGTT GGCCCTGGGG TTGGTGTCTG AGCGGAGGCA GCTATACAAG 
AAACGGAGAA AGAAGCTGGC GCCTTCTTGC ATGAATGGAG AAAAGTCCCC GAGAGACCCC 
TGGCAGGAAG ATCCGGAAGA GGAAGACGAA CACTTGCCAG AGGACGAAAG TGAAGATGAG 
GAGAGTGAGG AAGGCTTGCC ATCAGGACAG GGTCAGGGCA GCTCATCTCT CTCTGCTGAA 
AACCTCGGTG AGAGCCTTCG TCAGGAAGGC CTCTCTGCTC CCCTCCTGTG GCTCCAGAGC 
TCCCTGATCC GAGCAGCAAA TGACCGAGAA GAGGATGGCT GCTCCCAGGC AATCCCTCTG 
GTGCCTCTGA CAGAGGAAAA TGAGGAAGCA ATGGAGAACG AACAGTTTCA GCATCTGCTA 
CGCAAGCTAG GGATCCGGCC GCCCAGCTCA GGGCAGGAAA CCTTCTGGAG AATTCCAGCC 
AAACTGAGCT CCACCCAGCT TCGGAGGGTG GCTGCTTCTT TGAGTCAGCA AGAAAACGAG 
GAGGAAAGGG AAGAGGAGCC AGAGCCAGGA GTCCCCGGAG AGCAGGGTCC CAGTGAGGAG 
CACCGGACAG AAGCCCTGAG AGCCCTTCTG TCAGCCCGTA AGAGGAAAGC AGGCCTGGGG 
CCTACAGAAG AGGAGGCCAC TGGGGAGGAA GAATGGAACT CAGCGCCCAA GAAGCGGCAA 
CTGCTGGACA GCGACGAAGA GGAAGATGAT GAGGGGAGGA GGCAAGCAGT GTCGGGAACG 
CCAAGAGTCC ACAGGAAGAA ACGGTTTCAG ATTGAGGATG AGGATGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129554.1
CDS	181..3771
Translation	MDLYMMNCELLATCSALGYLEGGTYHKEPDCLESVKDLIRYLRHEDETRDVRQQLGAAQILQSDLLPILTQHRQDKPLFDAVIRLMVNLTQPALLCFGSVPKDSSVRHHFLQVLTYLQAYKEAFASEKAFGVLSETLYELLQLGWEDRQEEDNLLIERILLLVRNILHVPANLEQEKSIDDDASIHDRLLWAIHLSGMDDLLLFLSSSSAEQQWSLHVLEIISLMFRDQTPEQLAGVGQGRLAQERSTDVAELEVLRQREMAEKRARALQRGNRHSRFGGSYIVQGLKSIGEKDVVFHKGLHNLQNYSSDLGKQPRRVPKRRQAAQELSVHRRSVLNVRLFLRDFCSEFLENCYNPLMGAVKDHLLRERAQQHDETYYMWAMAFFMAFNRAATFRPGLVSETLSIRTFHFVEQNLTNYYEMMLTDRKEAASWARRMHLALKAYQELLATVNEMDMCPDEAVRESSRIIKNNIFYMMEYRELFLALFRKFDERYHPRSFLRDLVETTHLFLKMLERFCRSRGNLMVQNKRKKRKKKKKVQDQGVAFSQSPGELEAMWPALAEQLLQCAQDPELSVDPVVPFDAASEVPVEEQRVEAMVRIQDCLTAGQAPQALALLRSAREVWPEGNAFGSPVISPGEEMQLLKQILSTPLPRQQEPEEGDAEEEEEEEEEEELQVVQVSEKEFNFLEYLKRFASSTIVRAYVLLLRSYRQNSAHTNHCIAKMLHRLAHGLGMEALLFQLSLFCLFNRLLSDPAAAAYKELVTFAKYIIGKFFALAAVNQKAFVELLFWKNTAVVREMTQGYGSLDSGSSSHRAPLWSPEEEAQLQELYLAHKDVEGQDVVETILAHLKVVPRTRKQVIHHLVRMGLADSVKEFQKRKGTQIVLWTEDQELELQRLFEEFRDSDDVLGQIMKNITAKRSRARVVDKLLALGLVSERRQLYKKRRKKLAPSCMNGEKSPRDPWQEDPEEEDEHLPEDESEDEESEEGLPSGQGQGSSSLSAENLGESLRQEGLSAPLLWLQSSLIRAANDREEDGCSQAIPLVPLTEENEEAMENEQFQHLLRKLGIRPPSSGQETFWRIPAKLSSTQLRRVAASLSQQENEEEREEEPEPGVPGEQGPSEEHRTEALRALLSARKRKAGLGPTEEEATGEEEWNSAPKKRQLLDSDEEEDDEGRRQAVSGTPRVHRKKRFQIEDEDD

Target ORF information:

RefSeq Version	NM_001136082.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus timeless circadian clock 1 (Timeless), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001136082.2

ORF Insert Sequence:

ATGGACTTGT ACATGATGAA CTGTGAACTT CTAGCCACGT GTAGCGCCCT TGGGTACTTG 
GAAGGAGGGA CTTACCACAA GGAGCCGGAT TGCCTGGAGA GTGTGAAGGA TTTGATCCGA 
TACCTGAGGC ACGAGGATGA GACCCGAGAT GTGCGGCAGC AGCTGGGAGC TGCACAGATC 
CTGCAGAGCG ACCTCCTGCC AATCCTCACG CAGCATCGCC AGGACAAGCC TCTCTTCGAT 
GCCGTGATCA GGCTGATGGT AAATTTGACA CAGCCAGCCT TGCTCTGTTT TGGCAGCGTG 
CCTAAGGACT CCAGTGTACG GCACCATTTT CTGCAGGTTC TAACGTACCT GCAAGCCTAC 
AAAGAGGCCT TTGCCAGTGA GAAGGCATTT GGAGTCCTCA GCGAGACCTT GTATGAATTG 
CTACAGCTGG GCTGGGAGGA TCGGCAAGAA GAAGACAACT TGCTGATCGA GCGGATCCTT 
CTGCTGGTCA GAAATATTCT CCATGTCCCG GCCAACCTTG AGCAGGAGAA GAGTATCGAT 
GATGATGCCA GCATCCACGA CCGTCTCCTT TGGGCAATTC ACCTCAGTGG CATGGACGAC 
TTGCTCCTCT TCCTGTCCAG CTCATCCGCC GAGCAGCAGT GGAGCCTCCA TGTGCTGGAG 
ATCATCTCCC TCATGTTCCG AGACCAGACC CCTGAGCAGC TAGCGGGAGT AGGGCAGGGA 
CGCTTGGCTC AGGAGCGAAG CACGGATGTG GCAGAATTGG AGGTGCTGCG CCAACGGGAG 
ATGGCGGAGA AGAGAGCTCG GGCCCTCCAG CGAGGAAACA GGCACTCTCG ATTTGGGGGC 
TCCTACATTG TCCAGGGGTT GAAATCTATT GGGGAGAAGG ATGTCGTCTT TCACAAAGGC 
CTTCACAATC TCCAGAACTA CAGCTCAGAT CTGGGAAAGC AGCCCAGGAG GGTGCCCAAG 
CGTCGTCAGG CTGCCCAGGA GCTGTCTGTC CATCGCCGCT CTGTCCTGAA TGTGAGACTC 
TTCCTCAGAG ACTTCTGCTC TGAGTTCCTG GAGAACTGCT ACAACCCGCT CATGGGCGCG 
GTCAAGGATC ATCTGCTTCG GGAGAGAGCG CAGCAGCATG ACGAGACTTA CTACATGTGG 
GCAATGGCTT TCTTCATGGC CTTCAACCGA GCTGCCACCT TCCGCCCCGG CCTTGTTTCT 
GAGACCCTCA GTATCCGTAC CTTTCACTTT GTGGAGCAGA ACCTCACCAA CTACTACGAG 
ATGATGCTGA CAGACCGCAA GGAGGCCGCC TCCTGGGCGC GCAGGATGCA CCTGGCCCTG 
AAGGCCTACC AGGAGCTGCT GGCCACGGTG AACGAGATGG ACATGTGCCC AGATGAGGCT 
GTTAGGGAGA GCAGTCGTAT CATCAAAAAC AACATTTTCT ATATGATGGA GTACCGAGAA 
CTATTCCTGG CGCTCTTTCG AAAGTTTGAT GAGAGATACC ATCCACGCTC ATTCCTTCGA 
GACCTGGTGG AAACCACCCA CCTCTTCCTC AAAATGTTGG AGCGCTTTTG CCGGAGCCGC 
GGGAACCTGA TGGTGCAGAA CAAAAGAAAA AAGAGGAAAA AGAAAAAGAA GGTTCAGGAC 
CAGGGTGTTG CTTTCTCACA AAGCCCCGGG GAGCTGGAGG CCATGTGGCC AGCCCTGGCA 
GAGCAGCTGC TGCAGTGTGC CCAGGACCCT GAGCTCAGTG TGGACCCCGT CGTTCCCTTT 
GATGCGGCCT CAGAGGTGCC AGTGGAGGAG CAGCGGGTAG AAGCCATGGT GAGGATCCAA 
GACTGCCTTA CGGCTGGCCA GGCCCCGCAA GCCCTGGCCC TCCTGCGGTC TGCCCGGGAA 
GTGTGGCCTG AAGGAAATGC GTTTGGCTCT CCAGTCATTT CCCCAGGGGA AGAAATGCAG 
TTGCTGAAAC AAATCCTCTC CACGCCCCTT CCCCGGCAGC AGGAGCCAGA AGAAGGAGAT 
GCAGAGGAGG AAGAGGAAGA GGAGGAGGAA GAGGAGTTAC AGGTGGTCCA GGTGTCAGAG 
AAGGAGTTTA ACTTTCTGGA ATACCTGAAA CGCTTCGCAT CCTCAACCAT CGTGCGGGCC 
TACGTGCTTC TCCTGCGGAG CTACAGGCAG AACAGTGCTC ACACCAACCA CTGCATCGCC 
AAGATGCTGC ACCGGCTGGC CCATGGCCTG GGGATGGAAG CCCTGCTTTT CCAGCTGTCC 
CTGTTCTGCC TCTTCAATCG GCTGCTTAGT GACCCAGCTG CTGCGGCCTA CAAAGAGCTA 
GTGACTTTTG CCAAATACAT CATTGGCAAG TTCTTTGCGT TGGCTGCCGT GAACCAGAAA 
GCGTTTGTGG AGCTGCTGTT CTGGAAGAAC ACCGCAGTGG TTCGGGAAAT GACCCAGGGA 
TATGGCTCCC TCGACAGTGG GTCTTCCAGC CACAGAGCTC CTCTGTGGAG CCCTGAGGAA 
GAGGCCCAGC TTCAGGAACT ATACCTCGCC CACAAGGATG TGGAAGGTCA AGATGTAGTG 
GAAACCATAT TGGCGCACCT GAAAGTCGTT CCTCGAACAC GCAAGCAGGT CATCCACCAC 
CTGGTCCGGA TGGGCCTGGC CGACAGCGTC AAGGAGTTCC AGAAGAGGAA AGGGACCCAG 
ATTGTCTTGT GGACGGAGGA CCAGGAGCTG GAGTTACAGC GGCTCTTTGA GGAGTTCCGG 
GACTCTGATG ATGTTCTTGG TCAAATCATG AAGAATATCA CAGCCAAACG TTCACGGGCT 
CGAGTAGTGG ACAAACTGTT GGCCCTGGGG TTGGTGTCTG AGCGGAGGCA GCTATACAAG 
AAACGGAGAA AGAAGCTGGC GCCTTCTTGC ATGAATGGAG AAAAGTCCCC GAGAGACCCC 
TGGCAGGAAG ATCCGGAAGA GGAAGACGAA CACTTGCCAG AGGACGAAAG TGAAGATGAG 
GAGAGTGAGG AAGGCTTGCC ATCAGGACAG GGTCAGGGCA GCTCATCTCT CTCTGCTGAA 
AACCTCGGTG AGAGCCTTCG TCAGGAAGGC CTCTCTGCTC CCCTCCTGTG GCTCCAGAGC 
TCCCTGATCC GAGCAGCAAA TGACCGAGAA GAGGATGGCT GCTCCCAGGC AATCCCTCTG 
GTGCCTCTGA CAGAGGAAAA TGAGGAAGCA ATGGAGAACG AACAGTTTCA GCATCTGCTA 
CGCAAGCTAG GGATCCGGCC GCCCAGCTCA GGGCAGGAAA CCTTCTGGAG AATTCCAGCC 
AAACTGAGCT CCACCCAGCT TCGGAGGGTG GCTGCTTCTT TGAGTCAGCA AGAAAACGAG 
GAGGAAAGGG AAGAGGAGCC AGAGCCAGGA GTCCCCGGAG AGCAGGGTCC CAGTGAGGAG 
CACCGGACAG AAGCCCTGAG AGCCCTTCTG TCAGCCCGTA AGAGGAAAGC AGGCCTGGGG 
CCTACAGAAG AGGAGGCCAC TGGGGAGGAA GAATGGAACT CAGCGCCCAA GAAGCGGCAA 
CTGCTGGACA GCGACGAAGA GGAAGATGAT GAGGGGAGGA GGCAAGCAGT GTCGGGAACG 
CCAAGAGTCC ACAGGAAGAA ACGGTTTCAG ATTGAGGATG AGGATGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Timeless ( NM_001164080.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001157552.1 Mus musculus timeless circadian clock 1 (Timeless), transcript variant 3, mRNA.

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CloneID	OMu16574
Clone ID Related Accession (Same CDS sequence)	NM_001164080.1
Accession Version	NM_001164080.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3591bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-16
Organism	Mus musculus(house mouse)
Product	protein timeless homolog isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC052884.1, AK153838.1 and BC082770.1. Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 3' coding region, compared to variant 4. The resulting isoform (3) lacks one aa near the C-terminus, compared to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK153838.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849383, SAMN00849388 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACTTGT ACATGATGAA CTGTGAACTT CTAGCCACGT GTAGCGCCCT TGGGTACTTG 
GAAGGAGGGA CTTACCACAA GGAGCCGGAT TGCCTGGAGA GTGTGAAGGA TTTGATCCGA 
TACCTGAGGC ACGAGGATGA GACCCGAGAT GTGCGGCAGC AGCTGGGAGC TGCACAGATC 
CTGCAGAGCG ACCTCCTGCC AATCCTCACG CAGCATCGCC AGGACAAGCC TCTCTTCGAT 
GCCGTGATCA GGCTGATGGT AAATTTGACA CAGCCAGCCT TGCTCTGTTT TGGCAGCGTG 
CCTAAGGACT CCAGTGTACG GCACCATTTT CTGCAGGTTC TAACGTACCT GCAAGCCTAC 
AAAGAGGCCT TTGCCAGTGA GAAGGCATTT GGAGTCCTCA GCGAGACCTT GTATGAATTG 
CTACAGCTGG GCTGGGAGGA TCGGCAAGAA GAAGACAACT TGCTGATCGA GCGGATCCTT 
CTGCTGGTCA GAAATATTCT CCATGTCCCG GCCAACCTTG AGCAGGAGAA GAGTATCGAT 
GATGATGCCA GCATCCACGA CCGTCTCCTT TGGGCAATTC ACCTCAGTGG CATGGACGAC 
TTGCTCCTCT TCCTGTCCAG CTCATCCGCC GAGCAGCAGT GGAGCCTCCA TGTGCTGGAG 
ATCATCTCCC TCATGTTCCG AGACCAGACC CCTGAGCAGC TAGCGGGAGT AGGGCAGGGA 
CGCTTGGCTC AGGAGCGAAG CACGGATGTG GCAGAATTGG AGGTGCTGCG CCAACGGGAG 
ATGGCGGAGA AGAGAGCTCG GGCCCTCCAG CGAGGAAACA GGCACTCTCG ATTTGGGGGC 
TCCTACATTG TCCAGGGGTT GAAATCTATT GGGGAGAAGG ATGTCGTCTT TCACAAAGGC 
CTTCACAATC TCCAGAACTA CAGCTCAGAT CTGGGAAAGC AGCCCAGGAG GGTGCCCAAG 
CGTCGTCAGG CTGCCCAGGA GCTGTCTGTC CATCGCCGCT CTGTCCTGAA TGTGAGACTC 
TTCCTCAGAG ACTTCTGCTC TGAGTTCCTG GAGAACTGCT ACAACCCGCT CATGGGCGCG 
GTCAAGGATC ATCTGCTTCG GGAGAGAGCG CAGCAGCATG ACGAGACTTA CTACATGTGG 
GCAATGGCTT TCTTCATGGC CTTCAACCGA GCTGCCACCT TCCGCCCCGG CCTTGTTTCT 
GAGACCCTCA GTATCCGTAC CTTTCACTTT GTGGAGCAGA ACCTCACCAA CTACTACGAG 
ATGATGCTGA CAGACCGCAA GGAGGCCGCC TCCTGGGCGC GCAGGATGCA CCTGGCCCTG 
AAGGCCTACC AGGAGCTGCT GGCCACGGTG AACGAGATGG ACATGTGCCC AGATGAGGCT 
GTTAGGGAGA GCAGTCGTAT CATCAAAAAC AACATTTTCT ATATGATGGA GTACCGAGAA 
CTATTCCTGG CGCTCTTTCG AAAGTTTGAT GAGAGATACC ATCCACGCTC ATTCCTTCGA 
GACCTGGTGG AAACCACCCA CCTCTTCCTC AAAATGTTGG AGCGCTTTTG CCGGAGCCGC 
GGGAACCTGA TGGTGCAGAA CAAAAGAAAA AAGAGGAAAA AGAAAAAGAA GGTTCAGGAC 
CAGGGTGTTG CTTTCTCACA AAGCCCCGGG GAGCTGGAGG CCATGTGGCC AGCCCTGGCA 
GAGCAGCTGC TGCAGTGTGC CCAGGACCCT GAGCTCAGTG TGGACCCCGT CGTTCCCTTT 
GATGCGGCCT CAGAGGTGCC AGTGGAGGAG CAGCGGGTAG AAGCCATGGT GAGGATCCAA 
GACTGCCTTA CGGCTGGCCA GGCCCCGCAA GCCCTGGCCC TCCTGCGGTC TGCCCGGGAA 
GTGTGGCCTG AAGGAAATGC GTTTGGCTCT CCAGTCATTT CCCCAGGGGA AGAAATGCAG 
TTGCTGAAAC AAATCCTCTC CACGCCCCTT CCCCGGCAGC AGGAGCCAGA AGAAGGAGAT 
GCAGAGGAGG AAGAGGAAGA GGAGGAGGAA GAGGAGTTAC AGGTGGTCCA GGTGTCAGAG 
AAGGAGTTTA ACTTTCTGGA ATACCTGAAA CGCTTCGCAT CCTCAACCAT CGTGCGGGCC 
TACGTGCTTC TCCTGCGGAG CTACAGGCAG AACAGTGCTC ACACCAACCA CTGCATCGCC 
AAGATGCTGC ACCGGCTGGC CCATGGCCTG GGGATGGAAG CCCTGCTTTT CCAGCTGTCC 
CTGTTCTGCC TCTTCAATCG GCTGCTTAGT GACCCAGCTG CTGCGGCCTA CAAAGAGCTA 
GTGACTTTTG CCAAATACAT CATTGGCAAG TTCTTTGCGT TGGCTGCCGT GAACCAGAAA 
GCGTTTGTGG AGCTGCTGTT CTGGAAGAAC ACCGCAGTGG TTCGGGAAAT GACCCAGGGA 
TATGGCTCCC TCGACAGTGG GTCTTCCAGC CACAGAGCTC CTCTGTGGAG CCCTGAGGAA 
GAGGCCCAGC TTCAGGAACT ATACCTCGCC CACAAGGATG TGGAAGGTCA AGATGTAGTG 
GAAACCATAT TGGCGCACCT GAAAGTCGTT CCTCGAACAC GCAAGCAGGT CATCCACCAC 
CTGGTCCGGA TGGGCCTGGC CGACAGCGTC AAGGAGTTCC AGAAGAGGAA AGGGACCCAG 
ATTGTCTTGT GGACGGAGGA CCAGGAGCTG GAGTTACAGC GGCTCTTTGA GGAGTTCCGG 
GACTCTGATG ATGTTCTTGG TCAAATCATG AAGAATATCA CAGCCAAACG TTCACGGGCT 
CGAGTAGTGG ACAAACTGTT GGCCCTGGGG TTGGTGTCTG AGCGGAGGCA GCTATACAAG 
AAACGGAGAA AGAAGCTGGC GCCTTCTTGC ATGCAGAATG GAGAAAAGTC CCCGAGAGAC 
CCCTGGCAGG AAGATCCGGA AGAGGAAGAC GAACACTTGC CAGAGGACGA AAGTGAAGAT 
GAGGAGAGTG AGGAAGGCTT GCCATCAGGA CAGGGTCAGG GCAGCTCATC TCTCTCTGCT 
GAAAACCTCG GTGAGAGCCT TCGTCAGGAA GGCCTCTCTG CTCCCCTCCT GTGGCTCCAG 
AGCTCCCTGA TCCGAGCAGC AAATGACCGA GAAGAGGATG GCTGCTCCCA GGCAATCCCT 
CTGGTGCCTC TGACAGAGGA AAATGAGGAA GCAATGGAGA ACGAACAGTT TCAGCATCTG 
CTACGCAAGC TAGGGATCCG GCCGCCCAGC TCAGGGCAGG AAACCTTCTG GAGAATTCCA 
GCCAAACTGA GCTCCACCCA GCTTCGGAGG GTGGCTGCTT CTTTGAGTCA GCAAGAAAAC 
GAGGAGGAAA GGGAAGAGGA GCCAGAGCCA GGAGTCCCCG GAGAGCAGGG TCCCAGTGAG 
GAGCACCGGA CAGAAGCCCT GAGAGCCCTT CTGTCAGCCC GTAAGAGGAA AGCAGGCCTG 
GGGCCTACAG AAGAGGAGGC CACTGGGGAG GAAGAATGGA ACTCAGCGCC CAAGAAGCGG 
CAACTGCTGG ACAGCGACGA AGAGGAAGAT GATGAGGGGA GGAGGCAAGT GTCGGGAACG 
CCAAGAGTCC ACAGGAAGAA ACGGTTTCAG ATTGAGGATG AGGATGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001157552.1
CDS	181..3771
Translation	MDLYMMNCELLATCSALGYLEGGTYHKEPDCLESVKDLIRYLRHEDETRDVRQQLGAAQILQSDLLPILTQHRQDKPLFDAVIRLMVNLTQPALLCFGSVPKDSSVRHHFLQVLTYLQAYKEAFASEKAFGVLSETLYELLQLGWEDRQEEDNLLIERILLLVRNILHVPANLEQEKSIDDDASIHDRLLWAIHLSGMDDLLLFLSSSSAEQQWSLHVLEIISLMFRDQTPEQLAGVGQGRLAQERSTDVAELEVLRQREMAEKRARALQRGNRHSRFGGSYIVQGLKSIGEKDVVFHKGLHNLQNYSSDLGKQPRRVPKRRQAAQELSVHRRSVLNVRLFLRDFCSEFLENCYNPLMGAVKDHLLRERAQQHDETYYMWAMAFFMAFNRAATFRPGLVSETLSIRTFHFVEQNLTNYYEMMLTDRKEAASWARRMHLALKAYQELLATVNEMDMCPDEAVRESSRIIKNNIFYMMEYRELFLALFRKFDERYHPRSFLRDLVETTHLFLKMLERFCRSRGNLMVQNKRKKRKKKKKVQDQGVAFSQSPGELEAMWPALAEQLLQCAQDPELSVDPVVPFDAASEVPVEEQRVEAMVRIQDCLTAGQAPQALALLRSAREVWPEGNAFGSPVISPGEEMQLLKQILSTPLPRQQEPEEGDAEEEEEEEEEEELQVVQVSEKEFNFLEYLKRFASSTIVRAYVLLLRSYRQNSAHTNHCIAKMLHRLAHGLGMEALLFQLSLFCLFNRLLSDPAAAAYKELVTFAKYIIGKFFALAAVNQKAFVELLFWKNTAVVREMTQGYGSLDSGSSSHRAPLWSPEEEAQLQELYLAHKDVEGQDVVETILAHLKVVPRTRKQVIHHLVRMGLADSVKEFQKRKGTQIVLWTEDQELELQRLFEEFRDSDDVLGQIMKNITAKRSRARVVDKLLALGLVSERRQLYKKRRKKLAPSCMQNGEKSPRDPWQEDPEEEDEHLPEDESEDEESEEGLPSGQGQGSSSLSAENLGESLRQEGLSAPLLWLQSSLIRAANDREEDGCSQAIPLVPLTEENEEAMENEQFQHLLRKLGIRPPSSGQETFWRIPAKLSSTQLRRVAASLSQQENEEEREEEPEPGVPGEQGPSEEHRTEALRALLSARKRKAGLGPTEEEATGEEEWNSAPKKRQLLDSDEEEDDEGRRQVSGTPRVHRKKRFQIEDEDD

Target ORF information:

RefSeq Version	NM_001164080.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus timeless circadian clock 1 (Timeless), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001164080.1

ORF Insert Sequence:

ATGGACTTGT ACATGATGAA CTGTGAACTT CTAGCCACGT GTAGCGCCCT TGGGTACTTG 
GAAGGAGGGA CTTACCACAA GGAGCCGGAT TGCCTGGAGA GTGTGAAGGA TTTGATCCGA 
TACCTGAGGC ACGAGGATGA GACCCGAGAT GTGCGGCAGC AGCTGGGAGC TGCACAGATC 
CTGCAGAGCG ACCTCCTGCC AATCCTCACG CAGCATCGCC AGGACAAGCC TCTCTTCGAT 
GCCGTGATCA GGCTGATGGT AAATTTGACA CAGCCAGCCT TGCTCTGTTT TGGCAGCGTG 
CCTAAGGACT CCAGTGTACG GCACCATTTT CTGCAGGTTC TAACGTACCT GCAAGCCTAC 
AAAGAGGCCT TTGCCAGTGA GAAGGCATTT GGAGTCCTCA GCGAGACCTT GTATGAATTG 
CTACAGCTGG GCTGGGAGGA TCGGCAAGAA GAAGACAACT TGCTGATCGA GCGGATCCTT 
CTGCTGGTCA GAAATATTCT CCATGTCCCG GCCAACCTTG AGCAGGAGAA GAGTATCGAT 
GATGATGCCA GCATCCACGA CCGTCTCCTT TGGGCAATTC ACCTCAGTGG CATGGACGAC 
TTGCTCCTCT TCCTGTCCAG CTCATCCGCC GAGCAGCAGT GGAGCCTCCA TGTGCTGGAG 
ATCATCTCCC TCATGTTCCG AGACCAGACC CCTGAGCAGC TAGCGGGAGT AGGGCAGGGA 
CGCTTGGCTC AGGAGCGAAG CACGGATGTG GCAGAATTGG AGGTGCTGCG CCAACGGGAG 
ATGGCGGAGA AGAGAGCTCG GGCCCTCCAG CGAGGAAACA GGCACTCTCG ATTTGGGGGC 
TCCTACATTG TCCAGGGGTT GAAATCTATT GGGGAGAAGG ATGTCGTCTT TCACAAAGGC 
CTTCACAATC TCCAGAACTA CAGCTCAGAT CTGGGAAAGC AGCCCAGGAG GGTGCCCAAG 
CGTCGTCAGG CTGCCCAGGA GCTGTCTGTC CATCGCCGCT CTGTCCTGAA TGTGAGACTC 
TTCCTCAGAG ACTTCTGCTC TGAGTTCCTG GAGAACTGCT ACAACCCGCT CATGGGCGCG 
GTCAAGGATC ATCTGCTTCG GGAGAGAGCG CAGCAGCATG ACGAGACTTA CTACATGTGG 
GCAATGGCTT TCTTCATGGC CTTCAACCGA GCTGCCACCT TCCGCCCCGG CCTTGTTTCT 
GAGACCCTCA GTATCCGTAC CTTTCACTTT GTGGAGCAGA ACCTCACCAA CTACTACGAG 
ATGATGCTGA CAGACCGCAA GGAGGCCGCC TCCTGGGCGC GCAGGATGCA CCTGGCCCTG 
AAGGCCTACC AGGAGCTGCT GGCCACGGTG AACGAGATGG ACATGTGCCC AGATGAGGCT 
GTTAGGGAGA GCAGTCGTAT CATCAAAAAC AACATTTTCT ATATGATGGA GTACCGAGAA 
CTATTCCTGG CGCTCTTTCG AAAGTTTGAT GAGAGATACC ATCCACGCTC ATTCCTTCGA 
GACCTGGTGG AAACCACCCA CCTCTTCCTC AAAATGTTGG AGCGCTTTTG CCGGAGCCGC 
GGGAACCTGA TGGTGCAGAA CAAAAGAAAA AAGAGGAAAA AGAAAAAGAA GGTTCAGGAC 
CAGGGTGTTG CTTTCTCACA AAGCCCCGGG GAGCTGGAGG CCATGTGGCC AGCCCTGGCA 
GAGCAGCTGC TGCAGTGTGC CCAGGACCCT GAGCTCAGTG TGGACCCCGT CGTTCCCTTT 
GATGCGGCCT CAGAGGTGCC AGTGGAGGAG CAGCGGGTAG AAGCCATGGT GAGGATCCAA 
GACTGCCTTA CGGCTGGCCA GGCCCCGCAA GCCCTGGCCC TCCTGCGGTC TGCCCGGGAA 
GTGTGGCCTG AAGGAAATGC GTTTGGCTCT CCAGTCATTT CCCCAGGGGA AGAAATGCAG 
TTGCTGAAAC AAATCCTCTC CACGCCCCTT CCCCGGCAGC AGGAGCCAGA AGAAGGAGAT 
GCAGAGGAGG AAGAGGAAGA GGAGGAGGAA GAGGAGTTAC AGGTGGTCCA GGTGTCAGAG 
AAGGAGTTTA ACTTTCTGGA ATACCTGAAA CGCTTCGCAT CCTCAACCAT CGTGCGGGCC 
TACGTGCTTC TCCTGCGGAG CTACAGGCAG AACAGTGCTC ACACCAACCA CTGCATCGCC 
AAGATGCTGC ACCGGCTGGC CCATGGCCTG GGGATGGAAG CCCTGCTTTT CCAGCTGTCC 
CTGTTCTGCC TCTTCAATCG GCTGCTTAGT GACCCAGCTG CTGCGGCCTA CAAAGAGCTA 
GTGACTTTTG CCAAATACAT CATTGGCAAG TTCTTTGCGT TGGCTGCCGT GAACCAGAAA 
GCGTTTGTGG AGCTGCTGTT CTGGAAGAAC ACCGCAGTGG TTCGGGAAAT GACCCAGGGA 
TATGGCTCCC TCGACAGTGG GTCTTCCAGC CACAGAGCTC CTCTGTGGAG CCCTGAGGAA 
GAGGCCCAGC TTCAGGAACT ATACCTCGCC CACAAGGATG TGGAAGGTCA AGATGTAGTG 
GAAACCATAT TGGCGCACCT GAAAGTCGTT CCTCGAACAC GCAAGCAGGT CATCCACCAC 
CTGGTCCGGA TGGGCCTGGC CGACAGCGTC AAGGAGTTCC AGAAGAGGAA AGGGACCCAG 
ATTGTCTTGT GGACGGAGGA CCAGGAGCTG GAGTTACAGC GGCTCTTTGA GGAGTTCCGG 
GACTCTGATG ATGTTCTTGG TCAAATCATG AAGAATATCA CAGCCAAACG TTCACGGGCT 
CGAGTAGTGG ACAAACTGTT GGCCCTGGGG TTGGTGTCTG AGCGGAGGCA GCTATACAAG 
AAACGGAGAA AGAAGCTGGC GCCTTCTTGC ATGCAGAATG GAGAAAAGTC CCCGAGAGAC 
CCCTGGCAGG AAGATCCGGA AGAGGAAGAC GAACACTTGC CAGAGGACGA AAGTGAAGAT 
GAGGAGAGTG AGGAAGGCTT GCCATCAGGA CAGGGTCAGG GCAGCTCATC TCTCTCTGCT 
GAAAACCTCG GTGAGAGCCT TCGTCAGGAA GGCCTCTCTG CTCCCCTCCT GTGGCTCCAG 
AGCTCCCTGA TCCGAGCAGC AAATGACCGA GAAGAGGATG GCTGCTCCCA GGCAATCCCT 
CTGGTGCCTC TGACAGAGGA AAATGAGGAA GCAATGGAGA ACGAACAGTT TCAGCATCTG 
CTACGCAAGC TAGGGATCCG GCCGCCCAGC TCAGGGCAGG AAACCTTCTG GAGAATTCCA 
GCCAAACTGA GCTCCACCCA GCTTCGGAGG GTGGCTGCTT CTTTGAGTCA GCAAGAAAAC 
GAGGAGGAAA GGGAAGAGGA GCCAGAGCCA GGAGTCCCCG GAGAGCAGGG TCCCAGTGAG 
GAGCACCGGA CAGAAGCCCT GAGAGCCCTT CTGTCAGCCC GTAAGAGGAA AGCAGGCCTG 
GGGCCTACAG AAGAGGAGGC CACTGGGGAG GAAGAATGGA ACTCAGCGCC CAAGAAGCGG 
CAACTGCTGG ACAGCGACGA AGAGGAAGAT GATGAGGGGA GGAGGCAAGT GTCGGGAACG 
CCAAGAGTCC ACAGGAAGAA ACGGTTTCAG ATTGAGGATG AGGATGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Timeless ( XM_006513583.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006513646.1 Mus musculus timeless circadian clock 1 (Timeless), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu16520
Clone ID Related Accession (Same CDS sequence)	XM_006513583.3 , NM_001164081.1 , NM_011589.2
Accession Version	XM_006513583.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	protein timeless homolog isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006513583.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTTGT ACATGATGAA CTGTGAACTT CTAGCCACGT GTAGCGCCCT TGGGTACTTG 
GAAGGAGGGA CTTACCACAA GGAGCCGGAT TGCCTGGAGA GTGTGAAGGA TTTGATCCGA 
TACCTGAGGC ACGAGGATGA GACCCGAGAT GTGCGGCAGC AGCTGGGAGC TGCACAGATC 
CTGCAGAGCG ACCTCCTGCC AATCCTCACG CAGCATCGCC AGGACAAGCC TCTCTTCGAT 
GCCGTGATCA GGCTGATGGT AAATTTGACA CAGCCAGCCT TGCTCTGTTT TGGCAGCGTG 
CCTAAGGACT CCAGTGTACG GCACCATTTT CTGCAGGTTC TAACGTACCT GCAAGCCTAC 
AAAGAGGCCT TTGCCAGTGA GAAGGCATTT GGAGTCCTCA GCGAGACCTT GTATGAATTG 
CTACAGCTGG GCTGGGAGGA TCGGCAAGAA GAAGACAACT TGCTGATCGA GCGGATCCTT 
CTGCTGGTCA GAAATATTCT CCATGTCCCG GCCAACCTTG AGCAGGAGAA GAGTATCGAT 
GATGATGCCA GCATCCACGA CCGTCTCCTT TGGGCAATTC ACCTCAGTGG CATGGACGAC 
TTGCTCCTCT TCCTGTCCAG CTCATCCGCC GAGCAGCAGT GGAGCCTCCA TGTGCTGGAG 
ATCATCTCCC TCATGTTCCG AGACCAGACC CCTGAGCAGC TAGCGGGAGT AGGGCAGGGA 
CGCTTGGCTC AGGAGCGAAG CACGGATGTG GCAGAATTGG AGGTGCTGCG CCAACGGGAG 
ATGGCGGAGA AGAGAGCTCG GGCCCTCCAG CGAGGAAACA GGCACTCTCG ATTTGGGGGC 
TCCTACATTG TCCAGGGGTT GAAATCTATT GGGGAGAAGG ATGTCGTCTT TCACAAAGGC 
CTTCACAATC TCCAGAACTA CAGCTCAGAT CTGGGAAAGC AGCCCAGGAG GGTGCCCAAG 
CGTCGTCAGG CTGCCCAGGA GCTGTCTGTC CATCGCCGCT CTGTCCTGAA TGTGAGACTC 
TTCCTCAGAG ACTTCTGCTC TGAGTTCCTG GAGAACTGCT ACAACCCGCT CATGGGCGCG 
GTCAAGGATC ATCTGCTTCG GGAGAGAGCG CAGCAGCATG ACGAGACTTA CTACATGTGG 
GCAATGGCTT TCTTCATGGC CTTCAACCGA GCTGCCACCT TCCGCCCCGG CCTTGTTTCT 
GAGACCCTCA GTATCCGTAC CTTTCACTTT GTGGAGCAGA ACCTCACCAA CTACTACGAG 
ATGATGCTGA CAGACCGCAA GGAGGCCGCC TCCTGGGCGC GCAGGATGCA CCTGGCCCTG 
AAGGCCTACC AGGAGCTGCT GGCCACGGTG AACGAGATGG ACATGTGCCC AGATGAGGCT 
GTTAGGGAGA GCAGTCGTAT CATCAAAAAC AACATTTTCT ATATGATGGA GTACCGAGAA 
CTATTCCTGG CGCTCTTTCG AAAGTTTGAT GAGAGATACC ATCCACGCTC ATTCCTTCGA 
GACCTGGTGG AAACCACCCA CCTCTTCCTC AAAATGTTGG AGCGCTTTTG CCGGAGCCGC 
GGGAACCTGA TGGTGCAGAA CAAAAGAAAA AAGAGGAAAA AGAAAAAGAA GGTTCAGGAC 
CAGGGTGTTG CTTTCTCACA AAGCCCCGGG GAGCTGGAGG CCATGTGGCC AGCCCTGGCA 
GAGCAGCTGC TGCAGTGTGC CCAGGACCCT GAGCTCAGTG TGGACCCCGT CGTTCCCTTT 
GATGCGGCCT CAGAGGTGCC AGTGGAGGAG CAGCGGGTAG AAGCCATGGT GAGGATCCAA 
GACTGCCTTA CGGCTGGCCA GGCCCCGCAA GCCCTGGCCC TCCTGCGGTC TGCCCGGGAA 
GTGTGGCCTG AAGGAAATGC GTTTGGCTCT CCAGTCATTT CCCCAGGGGA AGAAATGCAG 
TTGCTGAAAC AAATCCTCTC CACGCCCCTT CCCCGGCAGC AGGAGCCAGA AGAAGGAGAT 
GCAGAGGAGG AAGAGGAAGA GGAGGAGGAA GAGGAGTTAC AGGTGGTCCA GGTGTCAGAG 
AAGGAGTTTA ACTTTCTGGA ATACCTGAAA CGCTTCGCAT CCTCAACCAT CGTGCGGGCC 
TACGTGCTTC TCCTGCGGAG CTACAGGCAG AACAGTGCTC ACACCAACCA CTGCATCGCC 
AAGATGCTGC ACCGGCTGGC CCATGGCCTG GGGATGGAAG CCCTGCTTTT CCAGCTGTCC 
CTGTTCTGCC TCTTCAATCG GCTGCTTAGT GACCCAGCTG CTGCGGCCTA CAAAGAGCTA 
GTGACTTTTG CCAAATACAT CATTGGCAAG TTCTTTGCGT TGGCTGCCGT GAACCAGAAA 
GCGTTTGTGG AGCTGCTGTT CTGGAAGAAC ACCGCAGTGG TTCGGGAAAT GACCCAGGGA 
TATGGCTCCC TCGACAGTGG GTCTTCCAGC CACAGAGCTC CTCTGTGGAG CCCTGAGGAA 
GAGGCCCAGC TTCAGGAACT ATACCTCGCC CACAAGGATG TGGAAGGTCA AGATGTAGTG 
GAAACCATAT TGGCGCACCT GAAAGTCGTT CCTCGAACAC GCAAGCAGGT CATCCACCAC 
CTGGTCCGGA TGGGCCTGGC CGACAGCGTC AAGGAGTTCC AGAAGAGGAA AGGGACCCAG 
ATTGTCTTGT GGACGGAGGA CCAGGAGCTG GAGTTACAGC GGCTCTTTGA GGAGTTCCGG 
GACTCTGATG ATGTTCTTGG TCAAATCATG AAGAATATCA CAGCCAAACG TTCACGGGCT 
CGAGTAGTGG ACAAACTGTT GGCCCTGGGG TTGGTGTCTG AGCGGAGGCA GCTATACAAG 
AAACGGAGAA AGAAGCTGGC GCCTTCTTGC ATGCAGAATG GAGAAAAGTC CCCGAGAGAC 
CCCTGGCAGG AAGATCCGGA AGAGGAAGAC GAACACTTGC CAGAGGACGA AAGTGAAGAT 
GAGGAGAGTG AGGAAGGCTT GCCATCAGGA CAGGGTCAGG GCAGCTCATC TCTCTCTGCT 
GAAAACCTCG GTGAGAGCCT TCGTCAGGAA GGCCTCTCTG CTCCCCTCCT GTGGCTCCAG 
AGCTCCCTGA TCCGAGCAGC AAATGACCGA GAAGAGGATG GCTGCTCCCA GGCAATCCCT 
CTGGTGCCTC TGACAGAGGA AAATGAGGAA GCAATGGAGA ACGAACAGTT TCAGCATCTG 
CTACGCAAGC TAGGGATCCG GCCGCCCAGC TCAGGGCAGG AAACCTTCTG GAGAATTCCA 
GCCAAACTGA GCTCCACCCA GCTTCGGAGG GTGGCTGCTT CTTTGAGTCA GCAAGAAAAC 
GAGGAGGAAA GGGAAGAGGA GCCAGAGCCA GGAGTCCCCG GAGAGCAGGG TCCCAGTGAG 
GAGCACCGGA CAGAAGCCCT GAGAGCCCTT CTGTCAGCCC GTAAGAGGAA AGCAGGCCTG 
GGGCCTACAG AAGAGGAGGC CACTGGGGAG GAAGAATGGA ACTCAGCGCC CAAGAAGCGG 
CAACTGCTGG ACAGCGACGA AGAGGAAGAT GATGAGGGGA GGAGGCAAGC AGTGTCGGGA 
ACGCCAAGAG TCCACAGGAA GAAACGGTTT CAGATTGAGG ATGAGGATGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006513646.1
CDS	1929..5522
Translation	MDLYMMNCELLATCSALGYLEGGTYHKEPDCLESVKDLIRYLRHEDETRDVRQQLGAAQILQSDLLPILTQHRQDKPLFDAVIRLMVNLTQPALLCFGSVPKDSSVRHHFLQVLTYLQAYKEAFASEKAFGVLSETLYELLQLGWEDRQEEDNLLIERILLLVRNILHVPANLEQEKSIDDDASIHDRLLWAIHLSGMDDLLLFLSSSSAEQQWSLHVLEIISLMFRDQTPEQLAGVGQGRLAQERSTDVAELEVLRQREMAEKRARALQRGNRHSRFGGSYIVQGLKSIGEKDVVFHKGLHNLQNYSSDLGKQPRRVPKRRQAAQELSVHRRSVLNVRLFLRDFCSEFLENCYNPLMGAVKDHLLRERAQQHDETYYMWAMAFFMAFNRAATFRPGLVSETLSIRTFHFVEQNLTNYYEMMLTDRKEAASWARRMHLALKAYQELLATVNEMDMCPDEAVRESSRIIKNNIFYMMEYRELFLALFRKFDERYHPRSFLRDLVETTHLFLKMLERFCRSRGNLMVQNKRKKRKKKKKVQDQGVAFSQSPGELEAMWPALAEQLLQCAQDPELSVDPVVPFDAASEVPVEEQRVEAMVRIQDCLTAGQAPQALALLRSAREVWPEGNAFGSPVISPGEEMQLLKQILSTPLPRQQEPEEGDAEEEEEEEEEEELQVVQVSEKEFNFLEYLKRFASSTIVRAYVLLLRSYRQNSAHTNHCIAKMLHRLAHGLGMEALLFQLSLFCLFNRLLSDPAAAAYKELVTFAKYIIGKFFALAAVNQKAFVELLFWKNTAVVREMTQGYGSLDSGSSSHRAPLWSPEEEAQLQELYLAHKDVEGQDVVETILAHLKVVPRTRKQVIHHLVRMGLADSVKEFQKRKGTQIVLWTEDQELELQRLFEEFRDSDDVLGQIMKNITAKRSRARVVDKLLALGLVSERRQLYKKRRKKLAPSCMQNGEKSPRDPWQEDPEEEDEHLPEDESEDEESEEGLPSGQGQGSSSLSAENLGESLRQEGLSAPLLWLQSSLIRAANDREEDGCSQAIPLVPLTEENEEAMENEQFQHLLRKLGIRPPSSGQETFWRIPAKLSSTQLRRVAASLSQQENEEEREEEPEPGVPGEQGPSEEHRTEALRALLSARKRKAGLGPTEEEATGEEEWNSAPKKRQLLDSDEEEDDEGRRQAVSGTPRVHRKKRFQIEDEDD

Target ORF information:

RefSeq Version	XM_006513583.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus timeless circadian clock 1 (Timeless), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006513583.3

ORF Insert Sequence:

ATGGACTTGT ACATGATGAA CTGTGAACTT CTAGCCACGT GTAGCGCCCT TGGGTACTTG 
GAAGGAGGGA CTTACCACAA GGAGCCGGAT TGCCTGGAGA GTGTGAAGGA TTTGATCCGA 
TACCTGAGGC ACGAGGATGA GACCCGAGAT GTGCGGCAGC AGCTGGGAGC TGCACAGATC 
CTGCAGAGCG ACCTCCTGCC AATCCTCACG CAGCATCGCC AGGACAAGCC TCTCTTCGAT 
GCCGTGATCA GGCTGATGGT AAATTTGACA CAGCCAGCCT TGCTCTGTTT TGGCAGCGTG 
CCTAAGGACT CCAGTGTACG GCACCATTTT CTGCAGGTTC TAACGTACCT GCAAGCCTAC 
AAAGAGGCCT TTGCCAGTGA GAAGGCATTT GGAGTCCTCA GCGAGACCTT GTATGAATTG 
CTACAGCTGG GCTGGGAGGA TCGGCAAGAA GAAGACAACT TGCTGATCGA GCGGATCCTT 
CTGCTGGTCA GAAATATTCT CCATGTCCCG GCCAACCTTG AGCAGGAGAA GAGTATCGAT 
GATGATGCCA GCATCCACGA CCGTCTCCTT TGGGCAATTC ACCTCAGTGG CATGGACGAC 
TTGCTCCTCT TCCTGTCCAG CTCATCCGCC GAGCAGCAGT GGAGCCTCCA TGTGCTGGAG 
ATCATCTCCC TCATGTTCCG AGACCAGACC CCTGAGCAGC TAGCGGGAGT AGGGCAGGGA 
CGCTTGGCTC AGGAGCGAAG CACGGATGTG GCAGAATTGG AGGTGCTGCG CCAACGGGAG 
ATGGCGGAGA AGAGAGCTCG GGCCCTCCAG CGAGGAAACA GGCACTCTCG ATTTGGGGGC 
TCCTACATTG TCCAGGGGTT GAAATCTATT GGGGAGAAGG ATGTCGTCTT TCACAAAGGC 
CTTCACAATC TCCAGAACTA CAGCTCAGAT CTGGGAAAGC AGCCCAGGAG GGTGCCCAAG 
CGTCGTCAGG CTGCCCAGGA GCTGTCTGTC CATCGCCGCT CTGTCCTGAA TGTGAGACTC 
TTCCTCAGAG ACTTCTGCTC TGAGTTCCTG GAGAACTGCT ACAACCCGCT CATGGGCGCG 
GTCAAGGATC ATCTGCTTCG GGAGAGAGCG CAGCAGCATG ACGAGACTTA CTACATGTGG 
GCAATGGCTT TCTTCATGGC CTTCAACCGA GCTGCCACCT TCCGCCCCGG CCTTGTTTCT 
GAGACCCTCA GTATCCGTAC CTTTCACTTT GTGGAGCAGA ACCTCACCAA CTACTACGAG 
ATGATGCTGA CAGACCGCAA GGAGGCCGCC TCCTGGGCGC GCAGGATGCA CCTGGCCCTG 
AAGGCCTACC AGGAGCTGCT GGCCACGGTG AACGAGATGG ACATGTGCCC AGATGAGGCT 
GTTAGGGAGA GCAGTCGTAT CATCAAAAAC AACATTTTCT ATATGATGGA GTACCGAGAA 
CTATTCCTGG CGCTCTTTCG AAAGTTTGAT GAGAGATACC ATCCACGCTC ATTCCTTCGA 
GACCTGGTGG AAACCACCCA CCTCTTCCTC AAAATGTTGG AGCGCTTTTG CCGGAGCCGC 
GGGAACCTGA TGGTGCAGAA CAAAAGAAAA AAGAGGAAAA AGAAAAAGAA GGTTCAGGAC 
CAGGGTGTTG CTTTCTCACA AAGCCCCGGG GAGCTGGAGG CCATGTGGCC AGCCCTGGCA 
GAGCAGCTGC TGCAGTGTGC CCAGGACCCT GAGCTCAGTG TGGACCCCGT CGTTCCCTTT 
GATGCGGCCT CAGAGGTGCC AGTGGAGGAG CAGCGGGTAG AAGCCATGGT GAGGATCCAA 
GACTGCCTTA CGGCTGGCCA GGCCCCGCAA GCCCTGGCCC TCCTGCGGTC TGCCCGGGAA 
GTGTGGCCTG AAGGAAATGC GTTTGGCTCT CCAGTCATTT CCCCAGGGGA AGAAATGCAG 
TTGCTGAAAC AAATCCTCTC CACGCCCCTT CCCCGGCAGC AGGAGCCAGA AGAAGGAGAT 
GCAGAGGAGG AAGAGGAAGA GGAGGAGGAA GAGGAGTTAC AGGTGGTCCA GGTGTCAGAG 
AAGGAGTTTA ACTTTCTGGA ATACCTGAAA CGCTTCGCAT CCTCAACCAT CGTGCGGGCC 
TACGTGCTTC TCCTGCGGAG CTACAGGCAG AACAGTGCTC ACACCAACCA CTGCATCGCC 
AAGATGCTGC ACCGGCTGGC CCATGGCCTG GGGATGGAAG CCCTGCTTTT CCAGCTGTCC 
CTGTTCTGCC TCTTCAATCG GCTGCTTAGT GACCCAGCTG CTGCGGCCTA CAAAGAGCTA 
GTGACTTTTG CCAAATACAT CATTGGCAAG TTCTTTGCGT TGGCTGCCGT GAACCAGAAA 
GCGTTTGTGG AGCTGCTGTT CTGGAAGAAC ACCGCAGTGG TTCGGGAAAT GACCCAGGGA 
TATGGCTCCC TCGACAGTGG GTCTTCCAGC CACAGAGCTC CTCTGTGGAG CCCTGAGGAA 
GAGGCCCAGC TTCAGGAACT ATACCTCGCC CACAAGGATG TGGAAGGTCA AGATGTAGTG 
GAAACCATAT TGGCGCACCT GAAAGTCGTT CCTCGAACAC GCAAGCAGGT CATCCACCAC 
CTGGTCCGGA TGGGCCTGGC CGACAGCGTC AAGGAGTTCC AGAAGAGGAA AGGGACCCAG 
ATTGTCTTGT GGACGGAGGA CCAGGAGCTG GAGTTACAGC GGCTCTTTGA GGAGTTCCGG 
GACTCTGATG ATGTTCTTGG TCAAATCATG AAGAATATCA CAGCCAAACG TTCACGGGCT 
CGAGTAGTGG ACAAACTGTT GGCCCTGGGG TTGGTGTCTG AGCGGAGGCA GCTATACAAG 
AAACGGAGAA AGAAGCTGGC GCCTTCTTGC ATGCAGAATG GAGAAAAGTC CCCGAGAGAC 
CCCTGGCAGG AAGATCCGGA AGAGGAAGAC GAACACTTGC CAGAGGACGA AAGTGAAGAT 
GAGGAGAGTG AGGAAGGCTT GCCATCAGGA CAGGGTCAGG GCAGCTCATC TCTCTCTGCT 
GAAAACCTCG GTGAGAGCCT TCGTCAGGAA GGCCTCTCTG CTCCCCTCCT GTGGCTCCAG 
AGCTCCCTGA TCCGAGCAGC AAATGACCGA GAAGAGGATG GCTGCTCCCA GGCAATCCCT 
CTGGTGCCTC TGACAGAGGA AAATGAGGAA GCAATGGAGA ACGAACAGTT TCAGCATCTG 
CTACGCAAGC TAGGGATCCG GCCGCCCAGC TCAGGGCAGG AAACCTTCTG GAGAATTCCA 
GCCAAACTGA GCTCCACCCA GCTTCGGAGG GTGGCTGCTT CTTTGAGTCA GCAAGAAAAC 
GAGGAGGAAA GGGAAGAGGA GCCAGAGCCA GGAGTCCCCG GAGAGCAGGG TCCCAGTGAG 
GAGCACCGGA CAGAAGCCCT GAGAGCCCTT CTGTCAGCCC GTAAGAGGAA AGCAGGCCTG 
GGGCCTACAG AAGAGGAGGC CACTGGGGAG GAAGAATGGA ACTCAGCGCC CAAGAAGCGG 
CAACTGCTGG ACAGCGACGA AGAGGAAGAT GATGAGGGGA GGAGGCAAGC AGTGTCGGGA 
ACGCCAAGAG TCCACAGGAA GAAACGGTTT CAGATTGAGG ATGAGGATGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Timeless ( XM_006513580.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006513643.1 Mus musculus timeless circadian clock 1 (Timeless), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu44454
Clone ID Related Accession (Same CDS sequence)	XM_006513580.2 , XM_006513580.1
Accession Version	XM_006513580.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3777bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein timeless homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACAGAA GCTACCTCAC CAGAGACCAG GTCTCTGAGA ACGGAAGCTA CCTCACCAGA 
GACCAGGTCT CTGAGAACGG GCCAGAAGAA AAGGCTCCTT CCAGTCCTGT CGTTCATCCC 
GTAGGCCTCA CCCTCTCCGT CCACCATCTC TACTGCCCGC TCTGCTGGTT GGGCCTCTGG 
TGTATGGACT TGTACATGAT GAACTGTGAA CTTCTAGCCA CGTGTAGCGC CCTTGGGTAC 
TTGGAAGGAG GGACTTACCA CAAGGAGCCG GATTGCCTGG AGAGTGTGAA GGATTTGATC 
CGATACCTGA GGCACGAGGA TGAGACCCGA GATGTGCGGC AGCAGCTGGG AGCTGCACAG 
ATCCTGCAGA GCGACCTCCT GCCAATCCTC ACGCAGCATC GCCAGGACAA GCCTCTCTTC 
GATGCCGTGA TCAGGCTGAT GGTAAATTTG ACACAGCCAG CCTTGCTCTG TTTTGGCAGC 
GTGCCTAAGG ACTCCAGTGT ACGGCACCAT TTTCTGCAGG TTCTAACGTA CCTGCAAGCC 
TACAAAGAGG CCTTTGCCAG TGAGAAGGCA TTTGGAGTCC TCAGCGAGAC CTTGTATGAA 
TTGCTACAGC TGGGCTGGGA GGATCGGCAA GAAGAAGACA ACTTGCTGAT CGAGCGGATC 
CTTCTGCTGG TCAGAAATAT TCTCCATGTC CCGGCCAACC TTGAGCAGGA GAAGAGTATC 
GATGATGATG CCAGCATCCA CGACCGTCTC CTTTGGGCAA TTCACCTCAG TGGCATGGAC 
GACTTGCTCC TCTTCCTGTC CAGCTCATCC GCCGAGCAGC AGTGGAGCCT CCATGTGCTG 
GAGATCATCT CCCTCATGTT CCGAGACCAG ACCCCTGAGC AGCTAGCGGG AGTAGGGCAG 
GGACGCTTGG CTCAGGAGCG AAGCACGGAT GTGGCAGAAT TGGAGGTGCT GCGCCAACGG 
GAGATGGCGG AGAAGAGAGC TCGGGCCCTC CAGCGAGGAA ACAGGCACTC TCGATTTGGG 
GGCTCCTACA TTGTCCAGGG GTTGAAATCT ATTGGGGAGA AGGATGTCGT CTTTCACAAA 
GGCCTTCACA ATCTCCAGAA CTACAGCTCA GATCTGGGAA AGCAGCCCAG GAGGGTGCCC 
AAGCGTCGTC AGGCTGCCCA GGAGCTGTCT GTCCATCGCC GCTCTGTCCT GAATGTGAGA 
CTCTTCCTCA GAGACTTCTG CTCTGAGTTC CTGGAGAACT GCTACAACCC GCTCATGGGC 
GCGGTCAAGG ATCATCTGCT TCGGGAGAGA GCGCAGCAGC ATGACGAGAC TTACTACATG 
TGGGCAATGG CTTTCTTCAT GGCCTTCAAC CGAGCTGCCA CCTTCCGCCC CGGCCTTGTT 
TCTGAGACCC TCAGTATCCG TACCTTTCAC TTTGTGGAGC AGAACCTCAC CAACTACTAC 
GAGATGATGC TGACAGACCG CAAGGAGGCC GCCTCCTGGG CGCGCAGGAT GCACCTGGCC 
CTGAAGGCCT ACCAGGAGCT GCTGGCCACG GTGAACGAGA TGGACATGTG CCCAGATGAG 
GCTGTTAGGG AGAGCAGTCG TATCATCAAA AACAACATTT TCTATATGAT GGAGTACCGA 
GAACTATTCC TGGCGCTCTT TCGAAAGTTT GATGAGAGAT ACCATCCACG CTCATTCCTT 
CGAGACCTGG TGGAAACCAC CCACCTCTTC CTCAAAATGT TGGAGCGCTT TTGCCGGAGC 
CGCGGGAACC TGATGGTGCA GAACAAAAGA AAAAAGAGGA AAAAGAAAAA GAAGGTTCAG 
GACCAGGGTG TTGCTTTCTC ACAAAGCCCC GGGGAGCTGG AGGCCATGTG GCCAGCCCTG 
GCAGAGCAGC TGCTGCAGTG TGCCCAGGAC CCTGAGCTCA GTGTGGACCC CGTCGTTCCC 
TTTGATGCGG CCTCAGAGGT GCCAGTGGAG GAGCAGCGGG TAGAAGCCAT GGTGAGGATC 
CAAGACTGCC TTACGGCTGG CCAGGCCCCG CAAGCCCTGG CCCTCCTGCG GTCTGCCCGG 
GAAGTGTGGC CTGAAGGAAA TGCGTTTGGC TCTCCAGTCA TTTCCCCAGG GGAAGAAATG 
CAGTTGCTGA AACAAATCCT CTCCACGCCC CTTCCCCGGC AGCAGGAGCC AGAAGAAGGA 
GATGCAGAGG AGGAAGAGGA AGAGGAGGAG GAAGAGGAGT TACAGGTGGT CCAGGTGTCA 
GAGAAGGAGT TTAACTTTCT GGAATACCTG AAACGCTTCG CATCCTCAAC CATCGTGCGG 
GCCTACGTGC TTCTCCTGCG GAGCTACAGG CAGAACAGTG CTCACACCAA CCACTGCATC 
GCCAAGATGC TGCACCGGCT GGCCCATGGC CTGGGGATGG AAGCCCTGCT TTTCCAGCTG 
TCCCTGTTCT GCCTCTTCAA TCGGCTGCTT AGTGACCCAG CTGCTGCGGC CTACAAAGAG 
CTAGTGACTT TTGCCAAATA CATCATTGGC AAGTTCTTTG CGTTGGCTGC CGTGAACCAG 
AAAGCGTTTG TGGAGCTGCT GTTCTGGAAG AACACCGCAG TGGTTCGGGA AATGACCCAG 
GGATATGGCT CCCTCGACAG TGGGTCTTCC AGCCACAGAG CTCCTCTGTG GAGCCCTGAG 
GAAGAGGCCC AGCTTCAGGA ACTATACCTC GCCCACAAGG ATGTGGAAGG TCAAGATGTA 
GTGGAAACCA TATTGGCGCA CCTGAAAGTC GTTCCTCGAA CACGCAAGCA GGTCATCCAC 
CACCTGGTCC GGATGGGCCT GGCCGACAGC GTCAAGGAGT TCCAGAAGAG GAAAGGGACC 
CAGATTGTCT TGTGGACGGA GGACCAGGAG CTGGAGTTAC AGCGGCTCTT TGAGGAGTTC 
CGGGACTCTG ATGATGTTCT TGGTCAAATC ATGAAGAATA TCACAGCCAA ACGTTCACGG 
GCTCGAGTAG TGGACAAACT GTTGGCCCTG GGGTTGGTGT CTGAGCGGAG GCAGCTATAC 
AAGAAACGGA GAAAGAAGCT GGCGCCTTCT TGCATGCAGA ATGGAGAAAA GTCCCCGAGA 
GACCCCTGGC AGGAAGATCC GGAAGAGGAA GACGAACACT TGCCAGAGGA CGAAAGTGAA 
GATGAGGAGA GTGAGGAAGG CTTGCCATCA GGACAGGGTC AGGGCAGCTC ATCTCTCTCT 
GCTGAAAACC TCGGTGAGAG CCTTCGTCAG GAAGGCCTCT CTGCTCCCCT CCTGTGGCTC 
CAGAGCTCCC TGATCCGAGC AGCAAATGAC CGAGAAGAGG ATGGCTGCTC CCAGGCAATC 
CCTCTGGTGC CTCTGACAGA GGAAAATGAG GAAGCAATGG AGAACGAACA GTTTCAGCAT 
CTGCTACGCA AGCTAGGGAT CCGGCCGCCC AGCTCAGGGC AGGAAACCTT CTGGAGAATT 
CCAGCCAAAC TGAGCTCCAC CCAGCTTCGG AGGGTGGCTG CTTCTTTGAG TCAGCAAGAA 
AACGAGGAGG AAAGGGAAGA GGAGCCAGAG CCAGGAGTCC CCGGAGAGCA GGGTCCCAGT 
GAGGAGCACC GGACAGAAGC CCTGAGAGCC CTTCTGTCAG CCCGTAAGAG GAAAGCAGGC 
CTGGGGCCTA CAGAAGAGGA GGCCACTGGG GAGGAAGAAT GGAACTCAGC GCCCAAGAAG 
CGGCAACTGC TGGACAGCGA CGAAGAGGAA GATGATGAGG GGAGGAGGCA AGCAGTGTCG 
GGAACGCCAA GAGTCCACAG GAAGAAACGG TTTCAGATTG AGGATGAGGA TGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006513643.1
CDS	1706..5482
Translation	MYRSYLTRDQVSENGSYLTRDQVSENGPEEKAPSSPVVHPVGLTLSVHHLYCPLCWLGLWCMDLYMMNCELLATCSALGYLEGGTYHKEPDCLESVKDLIRYLRHEDETRDVRQQLGAAQILQSDLLPILTQHRQDKPLFDAVIRLMVNLTQPALLCFGSVPKDSSVRHHFLQVLTYLQAYKEAFASEKAFGVLSETLYELLQLGWEDRQEEDNLLIERILLLVRNILHVPANLEQEKSIDDDASIHDRLLWAIHLSGMDDLLLFLSSSSAEQQWSLHVLEIISLMFRDQTPEQLAGVGQGRLAQERSTDVAELEVLRQREMAEKRARALQRGNRHSRFGGSYIVQGLKSIGEKDVVFHKGLHNLQNYSSDLGKQPRRVPKRRQAAQELSVHRRSVLNVRLFLRDFCSEFLENCYNPLMGAVKDHLLRERAQQHDETYYMWAMAFFMAFNRAATFRPGLVSETLSIRTFHFVEQNLTNYYEMMLTDRKEAASWARRMHLALKAYQELLATVNEMDMCPDEAVRESSRIIKNNIFYMMEYRELFLALFRKFDERYHPRSFLRDLVETTHLFLKMLERFCRSRGNLMVQNKRKKRKKKKKVQDQGVAFSQSPGELEAMWPALAEQLLQCAQDPELSVDPVVPFDAASEVPVEEQRVEAMVRIQDCLTAGQAPQALALLRSAREVWPEGNAFGSPVISPGEEMQLLKQILSTPLPRQQEPEEGDAEEEEEEEEEEELQVVQVSEKEFNFLEYLKRFASSTIVRAYVLLLRSYRQNSAHTNHCIAKMLHRLAHGLGMEALLFQLSLFCLFNRLLSDPAAAAYKELVTFAKYIIGKFFALAAVNQKAFVELLFWKNTAVVREMTQGYGSLDSGSSSHRAPLWSPEEEAQLQELYLAHKDVEGQDVVETILAHLKVVPRTRKQVIHHLVRMGLADSVKEFQKRKGTQIVLWTEDQELELQRLFEEFRDSDDVLGQIMKNITAKRSRARVVDKLLALGLVSERRQLYKKRRKKLAPSCMQNGEKSPRDPWQEDPEEEDEHLPEDESEDEESEEGLPSGQGQGSSSLSAENLGESLRQEGLSAPLLWLQSSLIRAANDREEDGCSQAIPLVPLTEENEEAMENEQFQHLLRKLGIRPPSSGQETFWRIPAKLSSTQLRRVAASLSQQENEEEREEEPEPGVPGEQGPSEEHRTEALRALLSARKRKAGLGPTEEEATGEEEWNSAPKKRQLLDSDEEEDDEGRRQAVSGTPRVHRKKRFQIEDEDD

Target ORF information:

RefSeq Version	XM_006513580.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus timeless circadian clock 1 (Timeless), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006513580.1

ORF Insert Sequence:

ATGTACAGAA GCTACCTCAC CAGAGACCAG GTCTCTGAGA ACGGAAGCTA CCTCACCAGA 
GACCAGGTCT CTGAGAACGG GCCAGAAGAA AAGGCTCCTT CCAGTCCTGT CGTTCATCCC 
GTAGGCCTCA CCCTCTCCGT CCACCATCTC TACTGCCCGC TCTGCTGGTT GGGCCTCTGG 
TGTATGGACT TGTACATGAT GAACTGTGAA CTTCTAGCCA CGTGTAGCGC CCTTGGGTAC 
TTGGAAGGAG GGACTTACCA CAAGGAGCCG GATTGCCTGG AGAGTGTGAA GGATTTGATC 
CGATACCTGA GGCACGAGGA TGAGACCCGA GATGTGCGGC AGCAGCTGGG AGCTGCACAG 
ATCCTGCAGA GCGACCTCCT GCCAATCCTC ACGCAGCATC GCCAGGACAA GCCTCTCTTC 
GATGCCGTGA TCAGGCTGAT GGTAAATTTG ACACAGCCAG CCTTGCTCTG TTTTGGCAGC 
GTGCCTAAGG ACTCCAGTGT ACGGCACCAT TTTCTGCAGG TTCTAACGTA CCTGCAAGCC 
TACAAAGAGG CCTTTGCCAG TGAGAAGGCA TTTGGAGTCC TCAGCGAGAC CTTGTATGAA 
TTGCTACAGC TGGGCTGGGA GGATCGGCAA GAAGAAGACA ACTTGCTGAT CGAGCGGATC 
CTTCTGCTGG TCAGAAATAT TCTCCATGTC CCGGCCAACC TTGAGCAGGA GAAGAGTATC 
GATGATGATG CCAGCATCCA CGACCGTCTC CTTTGGGCAA TTCACCTCAG TGGCATGGAC 
GACTTGCTCC TCTTCCTGTC CAGCTCATCC GCCGAGCAGC AGTGGAGCCT CCATGTGCTG 
GAGATCATCT CCCTCATGTT CCGAGACCAG ACCCCTGAGC AGCTAGCGGG AGTAGGGCAG 
GGACGCTTGG CTCAGGAGCG AAGCACGGAT GTGGCAGAAT TGGAGGTGCT GCGCCAACGG 
GAGATGGCGG AGAAGAGAGC TCGGGCCCTC CAGCGAGGAA ACAGGCACTC TCGATTTGGG 
GGCTCCTACA TTGTCCAGGG GTTGAAATCT ATTGGGGAGA AGGATGTCGT CTTTCACAAA 
GGCCTTCACA ATCTCCAGAA CTACAGCTCA GATCTGGGAA AGCAGCCCAG GAGGGTGCCC 
AAGCGTCGTC AGGCTGCCCA GGAGCTGTCT GTCCATCGCC GCTCTGTCCT GAATGTGAGA 
CTCTTCCTCA GAGACTTCTG CTCTGAGTTC CTGGAGAACT GCTACAACCC GCTCATGGGC 
GCGGTCAAGG ATCATCTGCT TCGGGAGAGA GCGCAGCAGC ATGACGAGAC TTACTACATG 
TGGGCAATGG CTTTCTTCAT GGCCTTCAAC CGAGCTGCCA CCTTCCGCCC CGGCCTTGTT 
TCTGAGACCC TCAGTATCCG TACCTTTCAC TTTGTGGAGC AGAACCTCAC CAACTACTAC 
GAGATGATGC TGACAGACCG CAAGGAGGCC GCCTCCTGGG CGCGCAGGAT GCACCTGGCC 
CTGAAGGCCT ACCAGGAGCT GCTGGCCACG GTGAACGAGA TGGACATGTG CCCAGATGAG 
GCTGTTAGGG AGAGCAGTCG TATCATCAAA AACAACATTT TCTATATGAT GGAGTACCGA 
GAACTATTCC TGGCGCTCTT TCGAAAGTTT GATGAGAGAT ACCATCCACG CTCATTCCTT 
CGAGACCTGG TGGAAACCAC CCACCTCTTC CTCAAAATGT TGGAGCGCTT TTGCCGGAGC 
CGCGGGAACC TGATGGTGCA GAACAAAAGA AAAAAGAGGA AAAAGAAAAA GAAGGTTCAG 
GACCAGGGTG TTGCTTTCTC ACAAAGCCCC GGGGAGCTGG AGGCCATGTG GCCAGCCCTG 
GCAGAGCAGC TGCTGCAGTG TGCCCAGGAC CCTGAGCTCA GTGTGGACCC CGTCGTTCCC 
TTTGATGCGG CCTCAGAGGT GCCAGTGGAG GAGCAGCGGG TAGAAGCCAT GGTGAGGATC 
CAAGACTGCC TTACGGCTGG CCAGGCCCCG CAAGCCCTGG CCCTCCTGCG GTCTGCCCGG 
GAAGTGTGGC CTGAAGGAAA TGCGTTTGGC TCTCCAGTCA TTTCCCCAGG GGAAGAAATG 
CAGTTGCTGA AACAAATCCT CTCCACGCCC CTTCCCCGGC AGCAGGAGCC AGAAGAAGGA 
GATGCAGAGG AGGAAGAGGA AGAGGAGGAG GAAGAGGAGT TACAGGTGGT CCAGGTGTCA 
GAGAAGGAGT TTAACTTTCT GGAATACCTG AAACGCTTCG CATCCTCAAC CATCGTGCGG 
GCCTACGTGC TTCTCCTGCG GAGCTACAGG CAGAACAGTG CTCACACCAA CCACTGCATC 
GCCAAGATGC TGCACCGGCT GGCCCATGGC CTGGGGATGG AAGCCCTGCT TTTCCAGCTG 
TCCCTGTTCT GCCTCTTCAA TCGGCTGCTT AGTGACCCAG CTGCTGCGGC CTACAAAGAG 
CTAGTGACTT TTGCCAAATA CATCATTGGC AAGTTCTTTG CGTTGGCTGC CGTGAACCAG 
AAAGCGTTTG TGGAGCTGCT GTTCTGGAAG AACACCGCAG TGGTTCGGGA AATGACCCAG 
GGATATGGCT CCCTCGACAG TGGGTCTTCC AGCCACAGAG CTCCTCTGTG GAGCCCTGAG 
GAAGAGGCCC AGCTTCAGGA ACTATACCTC GCCCACAAGG ATGTGGAAGG TCAAGATGTA 
GTGGAAACCA TATTGGCGCA CCTGAAAGTC GTTCCTCGAA CACGCAAGCA GGTCATCCAC 
CACCTGGTCC GGATGGGCCT GGCCGACAGC GTCAAGGAGT TCCAGAAGAG GAAAGGGACC 
CAGATTGTCT TGTGGACGGA GGACCAGGAG CTGGAGTTAC AGCGGCTCTT TGAGGAGTTC 
CGGGACTCTG ATGATGTTCT TGGTCAAATC ATGAAGAATA TCACAGCCAA ACGTTCACGG 
GCTCGAGTAG TGGACAAACT GTTGGCCCTG GGGTTGGTGT CTGAGCGGAG GCAGCTATAC 
AAGAAACGGA GAAAGAAGCT GGCGCCTTCT TGCATGCAGA ATGGAGAAAA GTCCCCGAGA 
GACCCCTGGC AGGAAGATCC GGAAGAGGAA GACGAACACT TGCCAGAGGA CGAAAGTGAA 
GATGAGGAGA GTGAGGAAGG CTTGCCATCA GGACAGGGTC AGGGCAGCTC ATCTCTCTCT 
GCTGAAAACC TCGGTGAGAG CCTTCGTCAG GAAGGCCTCT CTGCTCCCCT CCTGTGGCTC 
CAGAGCTCCC TGATCCGAGC AGCAAATGAC CGAGAAGAGG ATGGCTGCTC CCAGGCAATC 
CCTCTGGTGC CTCTGACAGA GGAAAATGAG GAAGCAATGG AGAACGAACA GTTTCAGCAT 
CTGCTACGCA AGCTAGGGAT CCGGCCGCCC AGCTCAGGGC AGGAAACCTT CTGGAGAATT 
CCAGCCAAAC TGAGCTCCAC CCAGCTTCGG AGGGTGGCTG CTTCTTTGAG TCAGCAAGAA 
AACGAGGAGG AAAGGGAAGA GGAGCCAGAG CCAGGAGTCC CCGGAGAGCA GGGTCCCAGT 
GAGGAGCACC GGACAGAAGC CCTGAGAGCC CTTCTGTCAG CCCGTAAGAG GAAAGCAGGC 
CTGGGGCCTA CAGAAGAGGA GGCCACTGGG GAGGAAGAAT GGAACTCAGC GCCCAAGAAG 
CGGCAACTGC TGGACAGCGA CGAAGAGGAA GATGATGAGG GGAGGAGGCA AGCAGTGTCG 
GGAACGCCAA GAGTCCACAG GAAGAAACGG TTTCAGATTG AGGATGAGGA TGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Timeless ( XM_006513581.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006513644.1 Mus musculus timeless circadian clock 1 (Timeless), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu44455
Clone ID Related Accession (Same CDS sequence)	XM_006513581.3 , XM_006513581.4
Accession Version	XM_006513581.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein timeless homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006513581.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACAGAA GCTACCTCAC CAGAGACCAG GTCTCTGAGA ACGGAAGCTA CCTCACCAGA 
GACCAGGTCT CTGAGAACGG GCCAGAAGAA AAGGCTCCTT CCAGTCCTGT CGTTCATCCC 
GTAGGCCTCA CCCTCTCCGT CCACCATCTC TACTGCCCGC TCTGCTGGTT GGGCCTCTGG 
TGTATGGACT TGTACATGAT GAACTGTGAA CTTCTAGCCA CGTGTAGCGC CCTTGGGTAC 
TTGGAAGGAG GGACTTACCA CAAGGAGCCG GATTGCCTGG AGAGTGTGAA GGATTTGATC 
CGATACCTGA GGCACGAGGA TGAGACCCGA GATGTGCGGC AGCAGCTGGG AGCTGCACAG 
ATCCTGCAGA GCGACCTCCT GCCAATCCTC ACGCAGCATC GCCAGGACAA GCCTCTCTTC 
GATGCCGTGA TCAGGCTGAT GGTAAATTTG ACACAGCCAG CCTTGCTCTG TTTTGGCAGC 
GTGCCTAAGG ACTCCAGTGT ACGGCACCAT TTTCTGCAGG TTCTAACGTA CCTGCAAGCC 
TACAAAGAGG CCTTTGCCAG TGAGAAGGCA TTTGGAGTCC TCAGCGAGAC CTTGTATGAA 
TTGCTACAGC TGGGCTGGGA GGATCGGCAA GAAGAAGACA ACTTGCTGAT CGAGCGGATC 
CTTCTGCTGG TCAGAAATAT TCTCCATGTC CCGGCCAACC TTGAGCAGGA GAAGAGTATC 
GATGATGATG CCAGCATCCA CGACCGTCTC CTTTGGGCAA TTCACCTCAG TGGCATGGAC 
GACTTGCTCC TCTTCCTGTC CAGCTCATCC GCCGAGCAGC AGTGGAGCCT CCATGTGCTG 
GAGATCATCT CCCTCATGTT CCGAGACCAG ACCCCTGAGC AGCTAGCGGG AGTAGGGCAG 
GGACGCTTGG CTCAGGAGCG AAGCACGGAT GTGGCAGAAT TGGAGGTGCT GCGCCAACGG 
GAGATGGCGG AGAAGAGAGC TCGGGCCCTC CAGCGAGGAA ACAGGCACTC TCGATTTGGG 
GGCTCCTACA TTGTCCAGGG GTTGAAATCT ATTGGGGAGA AGGATGTCGT CTTTCACAAA 
GGCCTTCACA ATCTCCAGAA CTACAGCTCA GATCTGGGAA AGCAGCCCAG GAGGGTGCCC 
AAGCGTCGTC AGGCTGCCCA GGAGCTGTCT GTCCATCGCC GCTCTGTCCT GAATGTGAGA 
CTCTTCCTCA GAGACTTCTG CTCTGAGTTC CTGGAGAACT GCTACAACCC GCTCATGGGC 
GCGGTCAAGG ATCATCTGCT TCGGGAGAGA GCGCAGCAGC ATGACGAGAC TTACTACATG 
TGGGCAATGG CTTTCTTCAT GGCCTTCAAC CGAGCTGCCA CCTTCCGCCC CGGCCTTGTT 
TCTGAGACCC TCAGTATCCG TACCTTTCAC TTTGTGGAGC AGAACCTCAC CAACTACTAC 
GAGATGATGC TGACAGACCG CAAGGAGGCC GCCTCCTGGG CGCGCAGGAT GCACCTGGCC 
CTGAAGGCCT ACCAGGAGCT GCTGGCCACG GTGAACGAGA TGGACATGTG CCCAGATGAG 
GCTGTTAGGG AGAGCAGTCG TATCATCAAA AACAACATTT TCTATATGAT GGAGTACCGA 
GAACTATTCC TGGCGCTCTT TCGAAAGTTT GATGAGAGAT ACCATCCACG CTCATTCCTT 
CGAGACCTGG TGGAAACCAC CCACCTCTTC CTCAAAATGT TGGAGCGCTT TTGCCGGAGC 
CGCGGGAACC TGATGGTGCA GAACAAAAGA AAAAAGAGGA AAAAGAAAAA GAAGGTTCAG 
GACCAGGGTG TTGCTTTCTC ACAAAGCCCC GGGGAGCTGG AGGCCATGTG GCCAGCCCTG 
GCAGAGCAGC TGCTGCAGTG TGCCCAGGAC CCTGAGCTCA GTGTGGACCC CGTCGTTCCC 
TTTGATGCGG CCTCAGAGGT GCCAGTGGAG GAGCAGCGGG TAGAAGCCAT GGTGAGGATC 
CAAGACTGCC TTACGGCTGG CCAGGCCCCG CAAGCCCTGG CCCTCCTGCG GTCTGCCCGG 
GAAGTGTGGC CTGAAGGAAA TGCGTTTGGC TCTCCAGTCA TTTCCCCAGG GGAAGAAATG 
CAGTTGCTGA AACAAATCCT CTCCACGCCC CTTCCCCGGC AGCAGGAGCC AGAAGAAGGA 
GATGCAGAGG AGGAAGAGGA AGAGGAGGAG GAAGAGGAGT TACAGGTGGT CCAGGTGTCA 
GAGAAGGAGT TTAACTTTCT GGAATACCTG AAACGCTTCG CATCCTCAAC CATCGTGCGG 
GCCTACGTGC TTCTCCTGCG GAGCTACAGG CAGAACAGTG CTCACACCAA CCACTGCATC 
GCCAAGATGC TGCACCGGCT GGCCCATGGC CTGGGGATGG AAGCCCTGCT TTTCCAGCTG 
TCCCTGTTCT GCCTCTTCAA TCGGCTGCTT AGTGACCCAG CTGCTGCGGC CTACAAAGAG 
CTAGTGACTT TTGCCAAATA CATCATTGGC AAGTTCTTTG CGTTGGCTGC CGTGAACCAG 
AAAGCGTTTG TGGAGCTGCT GTTCTGGAAG AACACCGCAG TGGTTCGGGA AATGACCCAG 
GGATATGGCT CCCTCGACAG TGGGTCTTCC AGCCACAGAG CTCCTCTGTG GAGCCCTGAG 
GAAGAGGCCC AGCTTCAGGA ACTATACCTC GCCCACAAGG ATGTGGAAGG TCAAGATGTA 
GTGGAAACCA TATTGGCGCA CCTGAAAGTC GTTCCTCGAA CACGCAAGCA GGTCATCCAC 
CACCTGGTCC GGATGGGCCT GGCCGACAGC GTCAAGGAGT TCCAGAAGAG GAAAGGGACC 
CAGATTGTCT TGTGGACGGA GGACCAGGAG CTGGAGTTAC AGCGGCTCTT TGAGGAGTTC 
CGGGACTCTG ATGATGTTCT TGGTCAAATC ATGAAGAATA TCACAGCCAA ACGTTCACGG 
GCTCGAGTAG TGGACAAACT GTTGGCCCTG GGGTTGGTGT CTGAGCGGAG GCAGCTATAC 
AAGAAACGGA GAAAGAAGCT GGCGCCTTCT TGCATGAATG GAGAAAAGTC CCCGAGAGAC 
CCCTGGCAGG AAGATCCGGA AGAGGAAGAC GAACACTTGC CAGAGGACGA AAGTGAAGAT 
GAGGAGAGTG AGGAAGGCTT GCCATCAGGA CAGGGTCAGG GCAGCTCATC TCTCTCTGCT 
GAAAACCTCG GTGAGAGCCT TCGTCAGGAA GGCCTCTCTG CTCCCCTCCT GTGGCTCCAG 
AGCTCCCTGA TCCGAGCAGC AAATGACCGA GAAGAGGATG GCTGCTCCCA GGCAATCCCT 
CTGGTGCCTC TGACAGAGGA AAATGAGGAA GCAATGGAGA ACGAACAGTT TCAGCATCTG 
CTACGCAAGC TAGGGATCCG GCCGCCCAGC TCAGGGCAGG AAACCTTCTG GAGAATTCCA 
GCCAAACTGA GCTCCACCCA GCTTCGGAGG GTGGCTGCTT CTTTGAGTCA GCAAGAAAAC 
GAGGAGGAAA GGGAAGAGGA GCCAGAGCCA GGAGTCCCCG GAGAGCAGGG TCCCAGTGAG 
GAGCACCGGA CAGAAGCCCT GAGAGCCCTT CTGTCAGCCC GTAAGAGGAA AGCAGGCCTG 
GGGCCTACAG AAGAGGAGGC CACTGGGGAG GAAGAATGGA ACTCAGCGCC CAAGAAGCGG 
CAACTGCTGG ACAGCGACGA AGAGGAAGAT GATGAGGGGA GGAGGCAAGC AGTGTCGGGA 
ACGCCAAGAG TCCACAGGAA GAAACGGTTT CAGATTGAGG ATGAGGATGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006513644.1
CDS	1706..5479
Translation	MYRSYLTRDQVSENGSYLTRDQVSENGPEEKAPSSPVVHPVGLTLSVHHLYCPLCWLGLWCMDLYMMNCELLATCSALGYLEGGTYHKEPDCLESVKDLIRYLRHEDETRDVRQQLGAAQILQSDLLPILTQHRQDKPLFDAVIRLMVNLTQPALLCFGSVPKDSSVRHHFLQVLTYLQAYKEAFASEKAFGVLSETLYELLQLGWEDRQEEDNLLIERILLLVRNILHVPANLEQEKSIDDDASIHDRLLWAIHLSGMDDLLLFLSSSSAEQQWSLHVLEIISLMFRDQTPEQLAGVGQGRLAQERSTDVAELEVLRQREMAEKRARALQRGNRHSRFGGSYIVQGLKSIGEKDVVFHKGLHNLQNYSSDLGKQPRRVPKRRQAAQELSVHRRSVLNVRLFLRDFCSEFLENCYNPLMGAVKDHLLRERAQQHDETYYMWAMAFFMAFNRAATFRPGLVSETLSIRTFHFVEQNLTNYYEMMLTDRKEAASWARRMHLALKAYQELLATVNEMDMCPDEAVRESSRIIKNNIFYMMEYRELFLALFRKFDERYHPRSFLRDLVETTHLFLKMLERFCRSRGNLMVQNKRKKRKKKKKVQDQGVAFSQSPGELEAMWPALAEQLLQCAQDPELSVDPVVPFDAASEVPVEEQRVEAMVRIQDCLTAGQAPQALALLRSAREVWPEGNAFGSPVISPGEEMQLLKQILSTPLPRQQEPEEGDAEEEEEEEEEEELQVVQVSEKEFNFLEYLKRFASSTIVRAYVLLLRSYRQNSAHTNHCIAKMLHRLAHGLGMEALLFQLSLFCLFNRLLSDPAAAAYKELVTFAKYIIGKFFALAAVNQKAFVELLFWKNTAVVREMTQGYGSLDSGSSSHRAPLWSPEEEAQLQELYLAHKDVEGQDVVETILAHLKVVPRTRKQVIHHLVRMGLADSVKEFQKRKGTQIVLWTEDQELELQRLFEEFRDSDDVLGQIMKNITAKRSRARVVDKLLALGLVSERRQLYKKRRKKLAPSCMNGEKSPRDPWQEDPEEEDEHLPEDESEDEESEEGLPSGQGQGSSSLSAENLGESLRQEGLSAPLLWLQSSLIRAANDREEDGCSQAIPLVPLTEENEEAMENEQFQHLLRKLGIRPPSSGQETFWRIPAKLSSTQLRRVAASLSQQENEEEREEEPEPGVPGEQGPSEEHRTEALRALLSARKRKAGLGPTEEEATGEEEWNSAPKKRQLLDSDEEEDDEGRRQAVSGTPRVHRKKRFQIEDEDD

Target ORF information:

RefSeq Version	XM_006513581.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus timeless circadian clock 1 (Timeless), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006513581.3

ORF Insert Sequence:

ATGTACAGAA GCTACCTCAC CAGAGACCAG GTCTCTGAGA ACGGAAGCTA CCTCACCAGA 
GACCAGGTCT CTGAGAACGG GCCAGAAGAA AAGGCTCCTT CCAGTCCTGT CGTTCATCCC 
GTAGGCCTCA CCCTCTCCGT CCACCATCTC TACTGCCCGC TCTGCTGGTT GGGCCTCTGG 
TGTATGGACT TGTACATGAT GAACTGTGAA CTTCTAGCCA CGTGTAGCGC CCTTGGGTAC 
TTGGAAGGAG GGACTTACCA CAAGGAGCCG GATTGCCTGG AGAGTGTGAA GGATTTGATC 
CGATACCTGA GGCACGAGGA TGAGACCCGA GATGTGCGGC AGCAGCTGGG AGCTGCACAG 
ATCCTGCAGA GCGACCTCCT GCCAATCCTC ACGCAGCATC GCCAGGACAA GCCTCTCTTC 
GATGCCGTGA TCAGGCTGAT GGTAAATTTG ACACAGCCAG CCTTGCTCTG TTTTGGCAGC 
GTGCCTAAGG ACTCCAGTGT ACGGCACCAT TTTCTGCAGG TTCTAACGTA CCTGCAAGCC 
TACAAAGAGG CCTTTGCCAG TGAGAAGGCA TTTGGAGTCC TCAGCGAGAC CTTGTATGAA 
TTGCTACAGC TGGGCTGGGA GGATCGGCAA GAAGAAGACA ACTTGCTGAT CGAGCGGATC 
CTTCTGCTGG TCAGAAATAT TCTCCATGTC CCGGCCAACC TTGAGCAGGA GAAGAGTATC 
GATGATGATG CCAGCATCCA CGACCGTCTC CTTTGGGCAA TTCACCTCAG TGGCATGGAC 
GACTTGCTCC TCTTCCTGTC CAGCTCATCC GCCGAGCAGC AGTGGAGCCT CCATGTGCTG 
GAGATCATCT CCCTCATGTT CCGAGACCAG ACCCCTGAGC AGCTAGCGGG AGTAGGGCAG 
GGACGCTTGG CTCAGGAGCG AAGCACGGAT GTGGCAGAAT TGGAGGTGCT GCGCCAACGG 
GAGATGGCGG AGAAGAGAGC TCGGGCCCTC CAGCGAGGAA ACAGGCACTC TCGATTTGGG 
GGCTCCTACA TTGTCCAGGG GTTGAAATCT ATTGGGGAGA AGGATGTCGT CTTTCACAAA 
GGCCTTCACA ATCTCCAGAA CTACAGCTCA GATCTGGGAA AGCAGCCCAG GAGGGTGCCC 
AAGCGTCGTC AGGCTGCCCA GGAGCTGTCT GTCCATCGCC GCTCTGTCCT GAATGTGAGA 
CTCTTCCTCA GAGACTTCTG CTCTGAGTTC CTGGAGAACT GCTACAACCC GCTCATGGGC 
GCGGTCAAGG ATCATCTGCT TCGGGAGAGA GCGCAGCAGC ATGACGAGAC TTACTACATG 
TGGGCAATGG CTTTCTTCAT GGCCTTCAAC CGAGCTGCCA CCTTCCGCCC CGGCCTTGTT 
TCTGAGACCC TCAGTATCCG TACCTTTCAC TTTGTGGAGC AGAACCTCAC CAACTACTAC 
GAGATGATGC TGACAGACCG CAAGGAGGCC GCCTCCTGGG CGCGCAGGAT GCACCTGGCC 
CTGAAGGCCT ACCAGGAGCT GCTGGCCACG GTGAACGAGA TGGACATGTG CCCAGATGAG 
GCTGTTAGGG AGAGCAGTCG TATCATCAAA AACAACATTT TCTATATGAT GGAGTACCGA 
GAACTATTCC TGGCGCTCTT TCGAAAGTTT GATGAGAGAT ACCATCCACG CTCATTCCTT 
CGAGACCTGG TGGAAACCAC CCACCTCTTC CTCAAAATGT TGGAGCGCTT TTGCCGGAGC 
CGCGGGAACC TGATGGTGCA GAACAAAAGA AAAAAGAGGA AAAAGAAAAA GAAGGTTCAG 
GACCAGGGTG TTGCTTTCTC ACAAAGCCCC GGGGAGCTGG AGGCCATGTG GCCAGCCCTG 
GCAGAGCAGC TGCTGCAGTG TGCCCAGGAC CCTGAGCTCA GTGTGGACCC CGTCGTTCCC 
TTTGATGCGG CCTCAGAGGT GCCAGTGGAG GAGCAGCGGG TAGAAGCCAT GGTGAGGATC 
CAAGACTGCC TTACGGCTGG CCAGGCCCCG CAAGCCCTGG CCCTCCTGCG GTCTGCCCGG 
GAAGTGTGGC CTGAAGGAAA TGCGTTTGGC TCTCCAGTCA TTTCCCCAGG GGAAGAAATG 
CAGTTGCTGA AACAAATCCT CTCCACGCCC CTTCCCCGGC AGCAGGAGCC AGAAGAAGGA 
GATGCAGAGG AGGAAGAGGA AGAGGAGGAG GAAGAGGAGT TACAGGTGGT CCAGGTGTCA 
GAGAAGGAGT TTAACTTTCT GGAATACCTG AAACGCTTCG CATCCTCAAC CATCGTGCGG 
GCCTACGTGC TTCTCCTGCG GAGCTACAGG CAGAACAGTG CTCACACCAA CCACTGCATC 
GCCAAGATGC TGCACCGGCT GGCCCATGGC CTGGGGATGG AAGCCCTGCT TTTCCAGCTG 
TCCCTGTTCT GCCTCTTCAA TCGGCTGCTT AGTGACCCAG CTGCTGCGGC CTACAAAGAG 
CTAGTGACTT TTGCCAAATA CATCATTGGC AAGTTCTTTG CGTTGGCTGC CGTGAACCAG 
AAAGCGTTTG TGGAGCTGCT GTTCTGGAAG AACACCGCAG TGGTTCGGGA AATGACCCAG 
GGATATGGCT CCCTCGACAG TGGGTCTTCC AGCCACAGAG CTCCTCTGTG GAGCCCTGAG 
GAAGAGGCCC AGCTTCAGGA ACTATACCTC GCCCACAAGG ATGTGGAAGG TCAAGATGTA 
GTGGAAACCA TATTGGCGCA CCTGAAAGTC GTTCCTCGAA CACGCAAGCA GGTCATCCAC 
CACCTGGTCC GGATGGGCCT GGCCGACAGC GTCAAGGAGT TCCAGAAGAG GAAAGGGACC 
CAGATTGTCT TGTGGACGGA GGACCAGGAG CTGGAGTTAC AGCGGCTCTT TGAGGAGTTC 
CGGGACTCTG ATGATGTTCT TGGTCAAATC ATGAAGAATA TCACAGCCAA ACGTTCACGG 
GCTCGAGTAG TGGACAAACT GTTGGCCCTG GGGTTGGTGT CTGAGCGGAG GCAGCTATAC 
AAGAAACGGA GAAAGAAGCT GGCGCCTTCT TGCATGAATG GAGAAAAGTC CCCGAGAGAC 
CCCTGGCAGG AAGATCCGGA AGAGGAAGAC GAACACTTGC CAGAGGACGA AAGTGAAGAT 
GAGGAGAGTG AGGAAGGCTT GCCATCAGGA CAGGGTCAGG GCAGCTCATC TCTCTCTGCT 
GAAAACCTCG GTGAGAGCCT TCGTCAGGAA GGCCTCTCTG CTCCCCTCCT GTGGCTCCAG 
AGCTCCCTGA TCCGAGCAGC AAATGACCGA GAAGAGGATG GCTGCTCCCA GGCAATCCCT 
CTGGTGCCTC TGACAGAGGA AAATGAGGAA GCAATGGAGA ACGAACAGTT TCAGCATCTG 
CTACGCAAGC TAGGGATCCG GCCGCCCAGC TCAGGGCAGG AAACCTTCTG GAGAATTCCA 
GCCAAACTGA GCTCCACCCA GCTTCGGAGG GTGGCTGCTT CTTTGAGTCA GCAAGAAAAC 
GAGGAGGAAA GGGAAGAGGA GCCAGAGCCA GGAGTCCCCG GAGAGCAGGG TCCCAGTGAG 
GAGCACCGGA CAGAAGCCCT GAGAGCCCTT CTGTCAGCCC GTAAGAGGAA AGCAGGCCTG 
GGGCCTACAG AAGAGGAGGC CACTGGGGAG GAAGAATGGA ACTCAGCGCC CAAGAAGCGG 
CAACTGCTGG ACAGCGACGA AGAGGAAGAT GATGAGGGGA GGAGGCAAGC AGTGTCGGGA 
ACGCCAAGAG TCCACAGGAA GAAACGGTTT CAGATTGAGG ATGAGGATGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Timeless ( XM_006513582.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006513645.1 Mus musculus timeless circadian clock 1 (Timeless), transcript variant X3, mRNA.

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CloneID	OMu44456
Clone ID Related Accession (Same CDS sequence)	XM_006513582.2 , XM_006513582.1
Accession Version	XM_006513582.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	protein timeless homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACAGAA GCTACCTCAC CAGAGACCAG GTCTCTGAGA ACGGAAGCTA CCTCACCAGA 
GACCAGGTCT CTGAGAACGG GCCAGAAGAA AAGGCTCCTT CCAGTCCTGT CGTTCATCCC 
GTAGGCCTCA CCCTCTCCGT CCACCATCTC TACTGCCCGC TCTGCTGGTT GGGCCTCTGG 
TGTATGGACT TGTACATGAT GAACTGTGAA CTTCTAGCCA CGTGTAGCGC CCTTGGGTAC 
TTGGAAGGAG GGACTTACCA CAAGGAGCCG GATTGCCTGG AGAGTGTGAA GGATTTGATC 
CGATACCTGA GGCACGAGGA TGAGACCCGA GATGTGCGGC AGCAGCTGGG AGCTGCACAG 
ATCCTGCAGA GCGACCTCCT GCCAATCCTC ACGCAGCATC GCCAGGACAA GCCTCTCTTC 
GATGCCGTGA TCAGGCTGAT GGTAAATTTG ACACAGCCAG CCTTGCTCTG TTTTGGCAGC 
GTGCCTAAGG ACTCCAGTGT ACGGCACCAT TTTCTGCAGG TTCTAACGTA CCTGCAAGCC 
TACAAAGAGG CCTTTGCCAG TGAGAAGGCA TTTGGAGTCC TCAGCGAGAC CTTGTATGAA 
TTGCTACAGC TGGGCTGGGA GGATCGGCAA GAAGAAGACA ACTTGCTGAT CGAGCGGATC 
CTTCTGCTGG TCAGAAATAT TCTCCATGTC CCGGCCAACC TTGAGCAGGA GAAGAGTATC 
GATGATGATG CCAGCATCCA CGACCGTCTC CTTTGGGCAA TTCACCTCAG TGGCATGGAC 
GACTTGCTCC TCTTCCTGTC CAGCTCATCC GCCGAGCAGC AGTGGAGCCT CCATGTGCTG 
GAGATCATCT CCCTCATGTT CCGAGACCAG ACCCCTGAGC AGCTAGCGGG AGTAGGGCAG 
GGACGCTTGG CTCAGGAGCG AAGCACGGAT GTGGCAGAAT TGGAGGTGCT GCGCCAACGG 
GAGATGGCGG AGAAGAGAGC TCGGGCCCTC CAGCGAGGAA ACAGGCACTC TCGATTTGGG 
GGCTCCTACA TTGTCCAGGG GTTGAAATCT ATTGGGGAGA AGGATGTCGT CTTTCACAAA 
GGCCTTCACA ATCTCCAGAA CTACAGCTCA GATCTGGGAA AGCAGCCCAG GAGGGTGCCC 
AAGCGTCGTC AGGCTGCCCA GGAGCTGTCT GTCCATCGCC GCTCTGTCCT GAATGTGAGA 
CTCTTCCTCA GAGACTTCTG CTCTGAGTTC CTGGAGAACT GCTACAACCC GCTCATGGGC 
GCGGTCAAGG ATCATCTGCT TCGGGAGAGA GCGCAGCAGC ATGACGAGAC TTACTACATG 
TGGGCAATGG CTTTCTTCAT GGCCTTCAAC CGAGCTGCCA CCTTCCGCCC CGGCCTTGTT 
TCTGAGACCC TCAGTATCCG TACCTTTCAC TTTGTGGAGC AGAACCTCAC CAACTACTAC 
GAGATGATGC TGACAGACCG CAAGGAGGCC GCCTCCTGGG CGCGCAGGAT GCACCTGGCC 
CTGAAGGCCT ACCAGGAGCT GCTGGCCACG GTGAACGAGA TGGACATGTG CCCAGATGAG 
GCTGTTAGGG AGAGCAGTCG TATCATCAAA AACAACATTT TCTATATGAT GGAGTACCGA 
GAACTATTCC TGGCGCTCTT TCGAAAGTTT GATGAGAGAT ACCATCCACG CTCATTCCTT 
CGAGACCTGG TGGAAACCAC CCACCTCTTC CTCAAAATGT TGGAGCGCTT TTGCCGGAGC 
CGCGGGAACC TGATGGTGCA GAACAAAAGA AAAAAGAGGA AAAAGAAAAA GAAGGTTCAG 
GACCAGGGTG TTGCTTTCTC ACAAAGCCCC GGGGAGCTGG AGGCCATGTG GCCAGCCCTG 
GCAGAGCAGC TGCTGCAGTG TGCCCAGGAC CCTGAGCTCA GTGTGGACCC CGTCGTTCCC 
TTTGATGCGG CCTCAGAGGT GCCAGTGGAG GAGCAGCGGG TAGAAGCCAT GGTGAGGATC 
CAAGACTGCC TTACGGCTGG CCAGGCCCCG CAAGCCCTGG CCCTCCTGCG GTCTGCCCGG 
GAAGTGTGGC CTGAAGGAAA TGCGTTTGGC TCTCCAGTCA TTTCCCCAGG GGAAGAAATG 
CAGTTGCTGA AACAAATCCT CTCCACGCCC CTTCCCCGGC AGCAGGAGCC AGAAGAAGGA 
GATGCAGAGG AGGAAGAGGA AGAGGAGGAG GAAGAGGAGT TACAGGTGGT CCAGGTGTCA 
GAGAAGGAGT TTAACTTTCT GGAATACCTG AAACGCTTCG CATCCTCAAC CATCGTGCGG 
GCCTACGTGC TTCTCCTGCG GAGCTACAGG CAGAACAGTG CTCACACCAA CCACTGCATC 
GCCAAGATGC TGCACCGGCT GGCCCATGGC CTGGGGATGG AAGCCCTGCT TTTCCAGCTG 
TCCCTGTTCT GCCTCTTCAA TCGGCTGCTT AGTGACCCAG CTGCTGCGGC CTACAAAGAG 
CTAGTGACTT TTGCCAAATA CATCATTGGC AAGTTCTTTG CGTTGGCTGC CGTGAACCAG 
AAAGCGTTTG TGGAGCTGCT GTTCTGGAAG AACACCGCAG TGGTTCGGGA AATGACCCAG 
GGATATGGCT CCCTCGACAG TGGGTCTTCC AGCCACAGAG CTCCTCTGTG GAGCCCTGAG 
GAAGAGGCCC AGCTTCAGGA ACTATACCTC GCCCACAAGG ATGTGGAAGG TCAAGATGTA 
GTGGAAACCA TATTGGCGCA CCTGAAAGTC GTTCCTCGAA CACGCAAGCA GGTCATCCAC 
CACCTGGTCC GGATGGGCCT GGCCGACAGC GTCAAGGAGT TCCAGAAGAG GAAAGGGACC 
CAGATTGTCT TGTGGACGGA GGACCAGGAG CTGGAGTTAC AGCGGCTCTT TGAGGAGTTC 
CGGGACTCTG ATGATGTTCT TGGTCAAATC ATGAAGAATA TCACAGCCAA ACGTTCACGG 
GCTCGAGTAG TGGACAAACT GTTGGCCCTG GGGTTGGTGT CTGAGCGGAG GCAGCTATAC 
AAGAAACGGA GAAAGAAGCT GGCGCCTTCT TGCATGCAGA ATGGAGAAAA GTCCCCGAGA 
GACCCCTGGC AGGAAGATCC GGAAGAGGAA GACGAACACT TGCCAGAGGA CGAAAGTGAA 
GATGAGGAGA GTGAGGAAGG CTTGCCATCA GGACAGGGTC AGGGCAGCTC ATCTCTCTCT 
GCTGAAAACC TCGGTGAGAG CCTTCGTCAG GAAGGCCTCT CTGCTCCCCT CCTGTGGCTC 
CAGAGCTCCC TGATCCGAGC AGCAAATGAC CGAGAAGAGG ATGGCTGCTC CCAGGCAATC 
CCTCTGGTGC CTCTGACAGA GGAAAATGAG GAAGCAATGG AGAACGAACA GTTTCAGCAT 
CTGCTACGCA AGCTAGGGAT CCGGCCGCCC AGCTCAGGGC AGGAAACCTT CTGGAGAATT 
CCAGCCAAAC TGAGCTCCAC CCAGCTTCGG AGGGTGGCTG CTTCTTTGAG TCAGCAAGAA 
AACGAGGAGG AAAGGGAAGA GGAGCCAGAG CCAGGAGTCC CCGGAGAGCA GGGTCCCAGT 
GAGGAGCACC GGACAGAAGC CCTGAGAGCC CTTCTGTCAG CCCGTAAGAG GAAAGCAGGC 
CTGGGGCCTA CAGAAGAGGA GGCCACTGGG GAGGAAGAAT GGAACTCAGC GCCCAAGAAG 
CGGCAACTGC TGGACAGCGA CGAAGAGGAA GATGATGAGG GGAGGAGGCA AGTGTCGGGA 
ACGCCAAGAG TCCACAGGAA GAAACGGTTT CAGATTGAGG ATGAGGATGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006513645.1
CDS	1706..5479
Translation	MYRSYLTRDQVSENGSYLTRDQVSENGPEEKAPSSPVVHPVGLTLSVHHLYCPLCWLGLWCMDLYMMNCELLATCSALGYLEGGTYHKEPDCLESVKDLIRYLRHEDETRDVRQQLGAAQILQSDLLPILTQHRQDKPLFDAVIRLMVNLTQPALLCFGSVPKDSSVRHHFLQVLTYLQAYKEAFASEKAFGVLSETLYELLQLGWEDRQEEDNLLIERILLLVRNILHVPANLEQEKSIDDDASIHDRLLWAIHLSGMDDLLLFLSSSSAEQQWSLHVLEIISLMFRDQTPEQLAGVGQGRLAQERSTDVAELEVLRQREMAEKRARALQRGNRHSRFGGSYIVQGLKSIGEKDVVFHKGLHNLQNYSSDLGKQPRRVPKRRQAAQELSVHRRSVLNVRLFLRDFCSEFLENCYNPLMGAVKDHLLRERAQQHDETYYMWAMAFFMAFNRAATFRPGLVSETLSIRTFHFVEQNLTNYYEMMLTDRKEAASWARRMHLALKAYQELLATVNEMDMCPDEAVRESSRIIKNNIFYMMEYRELFLALFRKFDERYHPRSFLRDLVETTHLFLKMLERFCRSRGNLMVQNKRKKRKKKKKVQDQGVAFSQSPGELEAMWPALAEQLLQCAQDPELSVDPVVPFDAASEVPVEEQRVEAMVRIQDCLTAGQAPQALALLRSAREVWPEGNAFGSPVISPGEEMQLLKQILSTPLPRQQEPEEGDAEEEEEEEEEEELQVVQVSEKEFNFLEYLKRFASSTIVRAYVLLLRSYRQNSAHTNHCIAKMLHRLAHGLGMEALLFQLSLFCLFNRLLSDPAAAAYKELVTFAKYIIGKFFALAAVNQKAFVELLFWKNTAVVREMTQGYGSLDSGSSSHRAPLWSPEEEAQLQELYLAHKDVEGQDVVETILAHLKVVPRTRKQVIHHLVRMGLADSVKEFQKRKGTQIVLWTEDQELELQRLFEEFRDSDDVLGQIMKNITAKRSRARVVDKLLALGLVSERRQLYKKRRKKLAPSCMQNGEKSPRDPWQEDPEEEDEHLPEDESEDEESEEGLPSGQGQGSSSLSAENLGESLRQEGLSAPLLWLQSSLIRAANDREEDGCSQAIPLVPLTEENEEAMENEQFQHLLRKLGIRPPSSGQETFWRIPAKLSSTQLRRVAASLSQQENEEEREEEPEPGVPGEQGPSEEHRTEALRALLSARKRKAGLGPTEEEATGEEEWNSAPKKRQLLDSDEEEDDEGRRQVSGTPRVHRKKRFQIEDEDD

Target ORF information:

RefSeq Version	XM_006513582.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus timeless circadian clock 1 (Timeless), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006513582.1

ORF Insert Sequence:

ATGTACAGAA GCTACCTCAC CAGAGACCAG GTCTCTGAGA ACGGAAGCTA CCTCACCAGA 
GACCAGGTCT CTGAGAACGG GCCAGAAGAA AAGGCTCCTT CCAGTCCTGT CGTTCATCCC 
GTAGGCCTCA CCCTCTCCGT CCACCATCTC TACTGCCCGC TCTGCTGGTT GGGCCTCTGG 
TGTATGGACT TGTACATGAT GAACTGTGAA CTTCTAGCCA CGTGTAGCGC CCTTGGGTAC 
TTGGAAGGAG GGACTTACCA CAAGGAGCCG GATTGCCTGG AGAGTGTGAA GGATTTGATC 
CGATACCTGA GGCACGAGGA TGAGACCCGA GATGTGCGGC AGCAGCTGGG AGCTGCACAG 
ATCCTGCAGA GCGACCTCCT GCCAATCCTC ACGCAGCATC GCCAGGACAA GCCTCTCTTC 
GATGCCGTGA TCAGGCTGAT GGTAAATTTG ACACAGCCAG CCTTGCTCTG TTTTGGCAGC 
GTGCCTAAGG ACTCCAGTGT ACGGCACCAT TTTCTGCAGG TTCTAACGTA CCTGCAAGCC 
TACAAAGAGG CCTTTGCCAG TGAGAAGGCA TTTGGAGTCC TCAGCGAGAC CTTGTATGAA 
TTGCTACAGC TGGGCTGGGA GGATCGGCAA GAAGAAGACA ACTTGCTGAT CGAGCGGATC 
CTTCTGCTGG TCAGAAATAT TCTCCATGTC CCGGCCAACC TTGAGCAGGA GAAGAGTATC 
GATGATGATG CCAGCATCCA CGACCGTCTC CTTTGGGCAA TTCACCTCAG TGGCATGGAC 
GACTTGCTCC TCTTCCTGTC CAGCTCATCC GCCGAGCAGC AGTGGAGCCT CCATGTGCTG 
GAGATCATCT CCCTCATGTT CCGAGACCAG ACCCCTGAGC AGCTAGCGGG AGTAGGGCAG 
GGACGCTTGG CTCAGGAGCG AAGCACGGAT GTGGCAGAAT TGGAGGTGCT GCGCCAACGG 
GAGATGGCGG AGAAGAGAGC TCGGGCCCTC CAGCGAGGAA ACAGGCACTC TCGATTTGGG 
GGCTCCTACA TTGTCCAGGG GTTGAAATCT ATTGGGGAGA AGGATGTCGT CTTTCACAAA 
GGCCTTCACA ATCTCCAGAA CTACAGCTCA GATCTGGGAA AGCAGCCCAG GAGGGTGCCC 
AAGCGTCGTC AGGCTGCCCA GGAGCTGTCT GTCCATCGCC GCTCTGTCCT GAATGTGAGA 
CTCTTCCTCA GAGACTTCTG CTCTGAGTTC CTGGAGAACT GCTACAACCC GCTCATGGGC 
GCGGTCAAGG ATCATCTGCT TCGGGAGAGA GCGCAGCAGC ATGACGAGAC TTACTACATG 
TGGGCAATGG CTTTCTTCAT GGCCTTCAAC CGAGCTGCCA CCTTCCGCCC CGGCCTTGTT 
TCTGAGACCC TCAGTATCCG TACCTTTCAC TTTGTGGAGC AGAACCTCAC CAACTACTAC 
GAGATGATGC TGACAGACCG CAAGGAGGCC GCCTCCTGGG CGCGCAGGAT GCACCTGGCC 
CTGAAGGCCT ACCAGGAGCT GCTGGCCACG GTGAACGAGA TGGACATGTG CCCAGATGAG 
GCTGTTAGGG AGAGCAGTCG TATCATCAAA AACAACATTT TCTATATGAT GGAGTACCGA 
GAACTATTCC TGGCGCTCTT TCGAAAGTTT GATGAGAGAT ACCATCCACG CTCATTCCTT 
CGAGACCTGG TGGAAACCAC CCACCTCTTC CTCAAAATGT TGGAGCGCTT TTGCCGGAGC 
CGCGGGAACC TGATGGTGCA GAACAAAAGA AAAAAGAGGA AAAAGAAAAA GAAGGTTCAG 
GACCAGGGTG TTGCTTTCTC ACAAAGCCCC GGGGAGCTGG AGGCCATGTG GCCAGCCCTG 
GCAGAGCAGC TGCTGCAGTG TGCCCAGGAC CCTGAGCTCA GTGTGGACCC CGTCGTTCCC 
TTTGATGCGG CCTCAGAGGT GCCAGTGGAG GAGCAGCGGG TAGAAGCCAT GGTGAGGATC 
CAAGACTGCC TTACGGCTGG CCAGGCCCCG CAAGCCCTGG CCCTCCTGCG GTCTGCCCGG 
GAAGTGTGGC CTGAAGGAAA TGCGTTTGGC TCTCCAGTCA TTTCCCCAGG GGAAGAAATG 
CAGTTGCTGA AACAAATCCT CTCCACGCCC CTTCCCCGGC AGCAGGAGCC AGAAGAAGGA 
GATGCAGAGG AGGAAGAGGA AGAGGAGGAG GAAGAGGAGT TACAGGTGGT CCAGGTGTCA 
GAGAAGGAGT TTAACTTTCT GGAATACCTG AAACGCTTCG CATCCTCAAC CATCGTGCGG 
GCCTACGTGC TTCTCCTGCG GAGCTACAGG CAGAACAGTG CTCACACCAA CCACTGCATC 
GCCAAGATGC TGCACCGGCT GGCCCATGGC CTGGGGATGG AAGCCCTGCT TTTCCAGCTG 
TCCCTGTTCT GCCTCTTCAA TCGGCTGCTT AGTGACCCAG CTGCTGCGGC CTACAAAGAG 
CTAGTGACTT TTGCCAAATA CATCATTGGC AAGTTCTTTG CGTTGGCTGC CGTGAACCAG 
AAAGCGTTTG TGGAGCTGCT GTTCTGGAAG AACACCGCAG TGGTTCGGGA AATGACCCAG 
GGATATGGCT CCCTCGACAG TGGGTCTTCC AGCCACAGAG CTCCTCTGTG GAGCCCTGAG 
GAAGAGGCCC AGCTTCAGGA ACTATACCTC GCCCACAAGG ATGTGGAAGG TCAAGATGTA 
GTGGAAACCA TATTGGCGCA CCTGAAAGTC GTTCCTCGAA CACGCAAGCA GGTCATCCAC 
CACCTGGTCC GGATGGGCCT GGCCGACAGC GTCAAGGAGT TCCAGAAGAG GAAAGGGACC 
CAGATTGTCT TGTGGACGGA GGACCAGGAG CTGGAGTTAC AGCGGCTCTT TGAGGAGTTC 
CGGGACTCTG ATGATGTTCT TGGTCAAATC ATGAAGAATA TCACAGCCAA ACGTTCACGG 
GCTCGAGTAG TGGACAAACT GTTGGCCCTG GGGTTGGTGT CTGAGCGGAG GCAGCTATAC 
AAGAAACGGA GAAAGAAGCT GGCGCCTTCT TGCATGCAGA ATGGAGAAAA GTCCCCGAGA 
GACCCCTGGC AGGAAGATCC GGAAGAGGAA GACGAACACT TGCCAGAGGA CGAAAGTGAA 
GATGAGGAGA GTGAGGAAGG CTTGCCATCA GGACAGGGTC AGGGCAGCTC ATCTCTCTCT 
GCTGAAAACC TCGGTGAGAG CCTTCGTCAG GAAGGCCTCT CTGCTCCCCT CCTGTGGCTC 
CAGAGCTCCC TGATCCGAGC AGCAAATGAC CGAGAAGAGG ATGGCTGCTC CCAGGCAATC 
CCTCTGGTGC CTCTGACAGA GGAAAATGAG GAAGCAATGG AGAACGAACA GTTTCAGCAT 
CTGCTACGCA AGCTAGGGAT CCGGCCGCCC AGCTCAGGGC AGGAAACCTT CTGGAGAATT 
CCAGCCAAAC TGAGCTCCAC CCAGCTTCGG AGGGTGGCTG CTTCTTTGAG TCAGCAAGAA 
AACGAGGAGG AAAGGGAAGA GGAGCCAGAG CCAGGAGTCC CCGGAGAGCA GGGTCCCAGT 
GAGGAGCACC GGACAGAAGC CCTGAGAGCC CTTCTGTCAG CCCGTAAGAG GAAAGCAGGC 
CTGGGGCCTA CAGAAGAGGA GGCCACTGGG GAGGAAGAAT GGAACTCAGC GCCCAAGAAG 
CGGCAACTGC TGGACAGCGA CGAAGAGGAA GATGATGAGG GGAGGAGGCA AGTGTCGGGA 
ACGCCAAGAG TCCACAGGAA GAAACGGTTT CAGATTGAGG ATGAGGATGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Timeless
Entrez Gene ID	21853
Full Name	timeless circadian clock 1
Synonyms	C77407,Debt69,tim
General protein information	Preferred Names timeless circadian clock 1 Names protein timeless homolog mTim timeless homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10 D3\|10 76.49 cM
Summary	The protein encoded by this gene is highly conserved and is involved in cell survival after damage or stress, increase in DNA polymerase epsilon activity, maintenance of telomere length, and epithelial cell morphogenesis. The encoded protein also plays a role in the circadian rhythm autoregulatory loop, interacting with the PERIOD genes (PER1, PER2, and PER3) and others to downregulate activation of PER1 by CLOCK/ARNTL. Changes in this gene or its expression may promote prostate cancer, lung cancer, breast cancer, and mental disorders. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2014].

Homo sapiens (human)	TIMELESS	NP_003911.2
Canis lupus familiaris (dog)	TIMELESS	XP_538233.3
Pan troglodytes (chimpanzee)	TIMELESS	XP_509535.2
	LOC712835	XP_002808027.1
Danio rerio (zebrafish)	timeless	NP_001265529.1
Xenopus tropicalis (tropical clawed frog)	timeless	XP_002934575.1
Bos taurus (cattle)	TIMELESS	NP_001095541.2
Mus musculus (house mouse)	Timeless	NP_035719.1
Rattus norvegicus (Norway rat)	Timeless	NP_112630.1
Drosophila melanogaster (fruit fly)	timeout	NP_524341.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010787	XP_001689129.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Timeless cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

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