Btf3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Btf3
Entrez Gene ID	218490
Full Name	basic transcription factor 3
Synonyms	1700054E11Rik
General protein information	Preferred Names basic transcription factor 3 Names transcription factor BTF3 NAC-beta RNA polymerase B transcription factor 3 nascent polypeptide-associated complex subunit beta
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 13 Map Location: 13\|13 D1

mRNA and Protein(s)

mRNA	Protein	Name
NM_145455.3	NP_663430.2	transcription factor BTF3 isoform 1
NM_001170540.1	NP_001164011.1	transcription factor BTF3 isoform 2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Btf3	NP_001008310.1
Arabidopsis thaliana (thale cress)	AT1G73230	NP_177466.1
	BTF3	XP_001100404.1
Canis lupus familiaris (dog)	BTF3	NP_001239062.1
Danio rerio (zebrafish)	btf3	NP_001070026.1
Drosophila melanogaster (fruit fly)	CG11835	NP_608532.1
Oryza sativa (rice)	Os03g0851000	NP_001051911.1
Homo sapiens (human)	BTF3	NP_001032726.1
Pan troglodytes (chimpanzee)	BTF3	XP_517710.1
Bos taurus (cattle)	BTF3	NP_001020485.1
Gallus gallus (chicken)	BTF3	XP_423823.4
Mus musculus (house mouse)	Btf3	NP_663430.2
Drosophila melanogaster (fruit fly)	bic	NP_725235.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006614	XP_316643.3
Arabidopsis thaliana (thale cress)	BTF3	NP_173230.1
Xenopus tropicalis (tropical clawed frog)	btf3	NP_001016697.1

Gene Ontology
Bibliography

Related articles in PubMed

Viral Infection-Induced Differential Expression of LncRNAs Associated with Collagen in Mouse Placentas and Amniotic Sacs.
Pan J, Mor G, Ju W, Zhong J, Luo X, Aldo PB, Zhong M, Yu Y, Jenkins EC, Brown WT, Zhong N
American journal of reproductive immunology (New York, N.Y. : 1989)74(3)237-57(2015 Sep)

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Yokoyama S, Ito Y, Ueno-Kudoh H, Shimizu H, Uchibe K, Albini S, Mitsuoka K, Miyaki S, Kiso M, Nagai A, Hikata T, Osada T, Fukuda N, Yamashita S, Harada D, Mezzano V, Kasai M, Puri PL, Hayashizaki Y, Okado H, Hashimoto M, Asahara H
Developmental cell17(6)836-48(2009 Dec)

A genome-wide screen for spatially restricted expression patterns identifies transcription factors that regulate glial development.
Fu H, Cai J, Clevers H, Fast E, Gray S, Greenberg R, Jain MK, Ma Q, Qiu M, Rowitch DH, Taylor CM, Stiles CD
The Journal of neuroscience : the official journal of the Society for Neuroscience29(36)11399-408(2009 Sep)

Genome-wide screening and identification of novel proteolytic cleavage targets of caspase-8 and -10 in vitro.
Bae S, Ha TS, Yoon Y, Lee J, Cha HJ, Yoo H, Choe TB, Li S, Sohn I, Kim JY, Kim CS, Jin HO, Lee HC, Park IC, Kim CS, Jin YW, Ahn SK
International journal of molecular medicine21(3)381-6(2008 Mar)

Genomic analysis of mouse retinal development.
Blackshaw S, Harpavat S, Trimarchi J, Cai L, Huang H, Kuo WP, Weber G, Lee K, Fraioli RE, Cho SH, Yung R, Asch E, Ohno-Machado L, Wong WH, Cepko CL
PLoS biology2(9)E247(2004 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Six putative caspase substrates, including five novel proteins (ABCF1, AKAP1, CPE, DOPEY1 and GOPC1) that may be targeted specifically by the initiator caspases 8 and 10 during the early stages of apoptosis, were identified.[caspase 10]
Title: Genome-wide screening and identification of novel proteolytic cleavage targets of caspase-8 and -10 in vitro.

The following Btf3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Btf3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu11605	NM_145455.3 Latest version!	Mus musculus basic transcription factor 3 (Btf3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu11604	NM_001170540.1 Latest version!	Mus musculus basic transcription factor 3 (Btf3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Btf3 ( NM_145455.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_663430.2 Mus musculus basic transcription factor 3 (Btf3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu11605
Clone ID Related Accession (Same CDS sequence)	NM_145455.3
Accession Version	NM_145455.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 615bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	transcription factor BTF3 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC064010.1. On Dec 23, 2009 this sequence version replaced NM_145455.2. Transcript Variant: This variant (1) represents the longer transcript and it encodes the longer protein (isoform 1). ##Evidence-Data-START## Transcript exon combination :: BC064010.1, BI411367.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGCGACGGA CGGGCGCTCC CACCCAGGCG GACTCTCGGG GGCGAGGGCG AGCCAGGGGC 
GGCTGGCCCG GGGCCGAAGC GACGCCCTCT CTCCCTCTCG GCGGAAGCCG AGGACGGGAG 
TCTCAGATGA AAGAAACAAT CATGAACCAG GAGAAACTCG CCAAACTGCA GGCACAAGTG 
CGCATTGGTG GGAAAGGAAC TGCTCGTAGG AAGAAGAAGG TGGTTCACAG AACGGCCACA 
GCAGACGATA AGAAACTGCA GTTCTCCTTA AAGAAGTTAG GGGTGAACAA CATCTCTGGT 
ATTGAAGAGG TGAACATGTT TACAAACCAA GGAACAGTGA TCCATTTTAA CAACCCTAAA 
GTTCAGGCAT CCCTGGCAGC AAACACCTTC ACCATTACAG GCCACGCTGA GACAAAGCAG 
CTGACAGAAA TGCTTCCCAG CATCCTCAAC CAGCTTGGTG CAGACAGCCT GACTAGTTTA 
AGGAGACTGG CTGAAGCTCT GCCCAAACAA TCTGTGGATG GAAAAGCACC CCTTGCTACT 
GGAGAGGATG ATGATGATGA AGTTCCAGAT CTGGTGGAGA ATTTTGATGA GGCTTCTAAG 
AATGAGGCAA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_663430.2
CDS	218..832
Translation	MRRTGAPTQADSRGRGRARGGWPGAEATPSLPLGGSRGRESQMKETIMNQEKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQFSLKKLGVNNISGIEEVNMFTNQGTVIHFNNPKVQASLAANTFTITGHAETKQLTEMLPSILNQLGADSLTSLRRLAEALPKQSVDGKAPLATGEDDDDEVPDLVENFDEASKNEAN

Target ORF information:

RefSeq Version	NM_145455.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus basic transcription factor 3 (Btf3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145455.3

ORF Insert Sequence:

ATGCGACGGA CGGGCGCTCC CACCCAGGCG GACTCTCGGG GGCGAGGGCG AGCCAGGGGC 
GGCTGGCCCG GGGCCGAAGC GACGCCCTCT CTCCCTCTCG GCGGAAGCCG AGGACGGGAG 
TCTCAGATGA AAGAAACAAT CATGAACCAG GAGAAACTCG CCAAACTGCA GGCACAAGTG 
CGCATTGGTG GGAAAGGAAC TGCTCGTAGG AAGAAGAAGG TGGTTCACAG AACGGCCACA 
GCAGACGATA AGAAACTGCA GTTCTCCTTA AAGAAGTTAG GGGTGAACAA CATCTCTGGT 
ATTGAAGAGG TGAACATGTT TACAAACCAA GGAACAGTGA TCCATTTTAA CAACCCTAAA 
GTTCAGGCAT CCCTGGCAGC AAACACCTTC ACCATTACAG GCCACGCTGA GACAAAGCAG 
CTGACAGAAA TGCTTCCCAG CATCCTCAAC CAGCTTGGTG CAGACAGCCT GACTAGTTTA 
AGGAGACTGG CTGAAGCTCT GCCCAAACAA TCTGTGGATG GAAAAGCACC CCTTGCTACT 
GGAGAGGATG ATGATGATGA AGTTCCAGAT CTGGTGGAGA ATTTTGATGA GGCTTCTAAG 
AATGAGGCAA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Btf3 ( NM_001170540.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001164011.1 Mus musculus basic transcription factor 3 (Btf3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu11604
Clone ID Related Accession (Same CDS sequence)	NM_001170540.1
Accession Version	NM_001170540.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-20
Organism	Mus musculus(house mouse)
Product	transcription factor BTF3 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ073405.1, AK139664.1 and BC064010.1. Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded protein (isoform 2) is shorter when it is compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: DV039128.1, DV047358.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGAAAGAAA CAATCATGAA CCAGGAGAAA CTCGCCAAAC TGCAGGCACA AGTGCGCATT 
GGTGGGAAAG GAACTGCTCG TAGGAAGAAG AAGGTGGTTC ACAGAACGGC CACAGCAGAC 
GATAAGAAAC TGCAGTTCTC CTTAAAGAAG TTAGGGGTGA ACAACATCTC TGGTATTGAA 
GAGGTGAACA TGTTTACAAA CCAAGGAACA GTGATCCATT TTAACAACCC TAAAGTTCAG 
GCATCCCTGG CAGCAAACAC CTTCACCATT ACAGGCCACG CTGAGACAAA GCAGCTGACA 
GAAATGCTTC CCAGCATCCT CAACCAGCTT GGTGCAGACA GCCTGACTAG TTTAAGGAGA 
CTGGCTGAAG CTCTGCCCAA ACAATCTGTG GATGGAAAAG CACCCCTTGC TACTGGAGAG 
GATGATGATG ATGAAGTTCC AGATCTGGTG GAGAATTTTG ATGAGGCTTC TAAGAATGAG 
GCAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001164011.1
CDS	142..630
Translation	MKETIMNQEKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQFSLKKLGVNNISGIEEVNMFTNQGTVIHFNNPKVQASLAANTFTITGHAETKQLTEMLPSILNQLGADSLTSLRRLAEALPKQSVDGKAPLATGEDDDDEVPDLVENFDEASKNEAN

Target ORF information:

RefSeq Version	NM_001170540.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus basic transcription factor 3 (Btf3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170540.1

ORF Insert Sequence:

ATGAAAGAAA CAATCATGAA CCAGGAGAAA CTCGCCAAAC TGCAGGCACA AGTGCGCATT 
GGTGGGAAAG GAACTGCTCG TAGGAAGAAG AAGGTGGTTC ACAGAACGGC CACAGCAGAC 
GATAAGAAAC TGCAGTTCTC CTTAAAGAAG TTAGGGGTGA ACAACATCTC TGGTATTGAA 
GAGGTGAACA TGTTTACAAA CCAAGGAACA GTGATCCATT TTAACAACCC TAAAGTTCAG 
GCATCCCTGG CAGCAAACAC CTTCACCATT ACAGGCCACG CTGAGACAAA GCAGCTGACA 
GAAATGCTTC CCAGCATCCT CAACCAGCTT GGTGCAGACA GCCTGACTAG TTTAAGGAGA 
CTGGCTGAAG CTCTGCCCAA ACAATCTGTG GATGGAAAAG CACCCCTTGC TACTGGAGAG 
GATGATGATG ATGAAGTTCC AGATCTGGTG GAGAATTTTG ATGAGGCTTC TAAGAATGAG 
GCAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Viral Infection-Induced Differential Expression of LncRNAs Associated with Collagen in Mouse Placentas and Amniotic Sacs.
American journal of reproductive immunology (New York, N.Y. : 1989)74(3)237-57(2015 Sep)
Pan J,Mor G,Ju W,Zhong J,Luo X,Aldo PB,Zhong M,Yu Y,Jenkins EC,Brown WT,Zhong N

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Developmental cell17(6)836-48(2009 Dec)
Yokoyama S,Ito Y,Ueno-Kudoh H,Shimizu H,Uchibe K,Albini S,Mitsuoka K,Miyaki S,Kiso M,Nagai A,Hikata T,Osada T,Fukuda N,Yamashita S,Harada D,Mezzano V,Kasai M,Puri PL,Hayashizaki Y,Okado H,Hashimoto M,Asahara H

A genome-wide screen for spatially restricted expression patterns identifies transcription factors that regulate glial development.
The Journal of neuroscience : the official journal of the Society for Neuroscience29(36)11399-408(2009 Sep)
Fu H,Cai J,Clevers H,Fast E,Gray S,Greenberg R,Jain MK,Ma Q,Qiu M,Rowitch DH,Taylor CM,Stiles CD

Genome-wide screening and identification of novel proteolytic cleavage targets of caspase-8 and -10 in vitro.
International journal of molecular medicine21(3)381-6(2008 Mar)
Bae S,Ha TS,Yoon Y,Lee J,Cha HJ,Yoo H,Choe TB,Li S,Sohn I,Kim JY,Kim CS,Jin HO,Lee HC,Park IC,Kim CS,Jin YW,Ahn SK

Genomic analysis of mouse retinal development.
PLoS biology2(9)E247(2004 Sep)
Blackshaw S,Harpavat S,Trimarchi J,Cai L,Huang H,Kuo WP,Weber G,Lee K,Fraioli RE,Cho SH,Yung R,Asch E,Ohno-Machado L,Wong WH,Cepko CL

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Btf3 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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