Rasa1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rasa1
Entrez Gene ID	218397
Full Name	RAS p21 protein activator 1
Synonyms	Gap,RasGAP,Rasa
General protein information	Preferred Names RAS p21 protein activator 1 Names ras GTPase-activating protein 1 GTPase-activating protein p120-rasGAP
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 13 Map Location: 13 C3\|13 45.05 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_011244518.2	XP_011242820.1	ras GTPase-activating protein 1 isoform X1
NM_145452.3	NP_663427.2	ras GTPase-activating protein 1
XM_011244518.3	XP_011242820.1	ras GTPase-activating protein 1 isoform X1

Pathways from BioSystems

Homologies

	RASA1	XP_001084074.2
Xenopus tropicalis (tropical clawed frog)	LOC100135092	NP_001107303.1
Pan troglodytes (chimpanzee)	RASA1	XP_517663.3
Canis lupus familiaris (dog)	RASA1	XP_536302.3
Bos taurus (cattle)	RASA1	NP_776874.1
Mus musculus (house mouse)	Rasa1	NP_663427.2
Rattus norvegicus (Norway rat)	Rasa1	NP_037267.1
Gallus gallus (chicken)	RASA1	XP_424907.4
Danio rerio (zebrafish)	rasa1a	XP_001342009.2
Drosophila melanogaster (fruit fly)	vap	NP_727926.2
Homo sapiens (human)	RASA1	NP_002881.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002729	XP_312197.3

Gene Ontology
Bibliography

Related articles in PubMed

RASA1 regulates the function of lymphatic vessel valves in mice.
Lapinski PE, Lubeck BA, Chen D, Doosti A, Zawieja SD, Davis MJ, King PD
The Journal of clinical investigation127(7)2569-2585(2017 Jun)

The caspase-3/p120 RasGAP stress-sensing module reduces liver cancer incidence but does not affect overall survival in gamma-irradiated and carcinogen-treated mice.
Vanli G, Sempoux C, Widmann C
Molecular carcinogenesis56(6)1680-1684(2017 Jun)

The caspase-3-p120-RasGAP module generates a NF-?B repressor in response to cellular stress.
Khalil H, Loukili N, Regamey A, Cuesta-Marban A, Santori E, Huber M, Widmann C
Journal of cell science128(18)3502-13(2015 Sep)

Cutting Edge: Codeletion of the Ras GTPase-Activating Proteins (RasGAPs) Neurofibromin 1 and p120 RasGAP in T Cells Results in the Development of T Cell Acute Lymphoblastic Leukemia.
Lubeck BA, Lapinski PE, Oliver JA, Ksionda O, Parada LF, Zhu Y, Maillard I, Chiang M, Roose J, King PD
Journal of immunology (Baltimore, Md. : 1950)195(1)31-5(2015 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

RASA1 catalytic activity is essential for the function and development of lymphatic vessel valves.
Title: RASA1 regulates the function of lymphatic vessel valves in mice.

These results indicate that the caspase-3/p120 RasGAP stress-sensing module impacts on carcinogen-induced liver cancer incidence but not sufficiently so as to affect overall survival.
Title: The caspase-3/p120 RasGAP stress-sensing module reduces liver cancer incidence but does not affect overall survival in gamma-irradiated and carcinogen-treated mice.

Double-deficient RASA1-neurofibromin 1 mice developed T cell acute lymphoblastic leukemia/lymphoma, which originated at an early point in T cell development and was dependent on activating mutations in the Notch1 gene.
Title: Cutting Edge: Codeletion of the Ras GTPase-Activating Proteins (RasGAPs) Neurofibromin 1 and p120 RasGAP in T Cells Results in the Development of T Cell Acute Lymphoblastic Leukemia.

Rasa1 may have a role in pathogenesis of capillary malformation-arteriovenous malformation in a mouse model
Title: Blood vascular abnormalities in Rasa1(R780Q) knockin mice: implications for the pathogenesis of capillary malformation-arteriovenous malformation.

Regulation of Rasa1 translation by miR-132 was seen in severed axons, demonstrating local function within the axon.
Title: MicroRNA-132 is enriched in developing axons, locally regulates Rasa1 mRNA, and promotes axon extension.

The following Rasa1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rasa1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu73058	XM_011244518.3 Latest version!	Mus musculus RAS p21 protein activator 1 (Rasa1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu13108	NM_145452.3 Latest version!	Mus musculus RAS p21 protein activator 1 (Rasa1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
View Old Accession Versions >>
OMu73058	XM_011244518.2	Mus musculus RAS p21 protein activator 1 (Rasa1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rasa1 ( XM_011244518.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011242820.1 Mus musculus RAS p21 protein activator 1 (Rasa1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu73058
Clone ID Related Accession (Same CDS sequence)	XM_011244518.2 , XM_011244518.3
Accession Version	XM_011244518.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2676bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ras GTPase-activating protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011244518.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCGG CCGAGGCGGG CAGTGAGGAA GGCGGCCCTG CGACGGCGGG CCCCGGCGGC 
GGCGCGGCCA CGGGCTCCAG CGCCTACTCG GCAGCGTGCC GGGTCAAGTT ACCCGCGGCC 
CTGCCGGTGG CAGCCGCCCC GTGTCCCGGG CTGGCGGACG CCGACTTGGT TGCGGCCCTC 
GGTGGCGGAG CCGCGTCGGG GTCCGGGTTC CTGGGGACCG GGCCGGTGGC CGGTGTCTTG 
GGGGGCGCCG CGCTGACCGG CAGTGCTGCT GCTGGCGTGG CTGGTGCTGC GGCCGCCGGA 
CCTGCGGGAG ACGTCGCTTT CACCAAGGGG ACTCTGTCTT TGCCTGCTGA GACGCTCGGG 
CCGGGCGGCG GATTCCCTCC TCTGCCGCCG CCTCCTCAGC TGCCCCCTTT GGGCTCCGGT 
TTGGGGACCG TGGATGAAGG TGACTCCCTG GATGGACCAG AATACGAAGA GGAAGAGGTG 
GCCATACCGC TGACCGCACC TCCCACTAAC CAGTGGTATC ATGGAAAACT TGATAGAACT 
ATAGCAGAAG AACGCCTCAG GCAGGCAGGG AAATCTGGAA GCTATCTTAT AAGAGAGAGT 
GATCGGAGGC CGGGGTCCTT TGTACTTTCA TTCCTTAGTC AGACAAATGT TGTCAATCAT 
TTTAGGATTA TTGCTATGTG TGGTGATTAC TACATTGGTG GAAGGCGTTT TTCTTCACTC 
TCAGACCTAA TAGGTTATTA CAGTCACGTT TCTTGTTTAC TTAAAGGAGA AAAATTACTC 
TATCCAGTTG CACCACCAGA GCCAGTAGAA GATAGAAGGC GTGTACGAGC CATTCTGCCC 
TACACAAAAG TACCAGACAC TGATGAAATA AGTTTTTTAA AAGGAGATAT GTTCATTGTT 
CACAATGAGT TAGAAGATGG ATGGATGTGG GTTACCAATC TAAGAACAGA TGAGCAAGGC 
CTTATTGTTG AAGACCTAGT GGAAGAGGTG GGCCGAGAAG AAGATCCACA CGAAGGCAAA 
ATATGGTTCC ACGGGAAAAT TTCCAAACAA GAAGCTTATA ATTTATTAAT GACAGTTGGA 
CAAGTCTGCA GCTTTCTTGT GAGGCCCTCA GATAATACTC CTGGAGATTA TTCTCTGTAT 
TTTCGGACCA ATGAAAATAT TCAGCGATTT AAAATTTGTC CAACACCTAA CAACCAGTTT 
ATGATGGGAG GCCGCTATTA TAACAGTATC GGGGACATCA TAGACCATTA TCGGAAAGAA 
CAGATTGTTG AAGGCTATTA TCTTAAGGAA CCTGTACCAA TGCAGGATCA AGGGCAAGTA 
CTGAACGACA CTGTGGATGG GAAGGAGATC TATAACACAA TTCGTCGGAA GACAAAGGAT 
GCTTTTTATA AAAATATTGT TAAAAAAGGT TATCTTCTGA AGAAGGGTAA AGGAAAACGT 
TGGAAAAATT TATATTTTAT CTTAGAAGGC AGTGATGCTC AACTTATTTA TTTTGAAAGT 
GAAAAACGAG CTACAAAACC AAAAGGGCTA ATAGACCTTA GTGTGTGTTC TGTCTACGTT 
GTCCATGACA GTCTCTTCGG CAGGCCAAAC TGTTTTCAGA TAGTAGTTCA GCACTTTAGT 
GAAGAACATT ACATCTTTTA CTTTGCAGGA GAAACTCCAG AACAAGCAGA GGATTGGATG 
AAAGGTCTGC AAGCATTTTG CAGTTTACGG AAAAGTAGCC CAGGGACATC TAATAAACGC 
CTTCGTCAGG TCAGCAGCCT TGTTTTACAT ATTGAAGAAG CCCATAAACT ACCAGTAAAA 
CACTTTACTA ATCCATATTG TAACATCTAC TTGAATAGCG TCCAAGTAGC AAAAACTCAT 
GCAAGGGAGG GGCAAAACCC AGTATGGTCA GAGGAGTTTG TCTTTGATGA TCTTCCTCCT 
GACATCAATA GATTTGAAAT CACACTTAGT AATAAAACAA AGAAAAGCAA AGATCCAGAT 
ATCTTATTTA TGCGTTGCCA GTTGAGCAGG TTACAGAAAG GACATGCAAC AGATGAATGG 
TTTCTACTAA GTTCCCATAT ACCACTAAAA GGTATTGAAC CAGGATCTTT GCGTGTTCGA 
GCACGATACT CCATGGAAAA AATCATGCCA GAAGAAGAGT ACAGTGAATT TAAAGAGCTT 
ATACTGCAAA AGGAACTTCA TGTCGTCTAT GCTTTATCAC ATGTATGTGG ACAAGACCGA 
ACACTACTGG CCAGCATCCT ACTAAAGATA TTTCTTCATG AAAAGCTTGA ATCATTGTTG 
TTATGCACAC TAAATGACAG AGAGATAAGT ATGGAAGATG AAGCCACTAC CCTATTTCGA 
GCTACAACAC TTGCAAGTAC CTTGATGGAG CAGTATATGA AAGCTACTGC CACACAGTTT 
GTTCATCATG CTTTGAAAGA CTCCATTTTA AAGATTATGG AAAGCAAGCA ATCATGTGAG 
TTAAGTCCGT CAAAGTTAGA AAAAAATGAA GATGTGAACA CTAATTTAGC ACACCTATTG 
AGCATACTTT CAGAGCTTGT GGAGAAAATA TTCATGGCTT CAGAAATACT TCCACCGACA 
CTGAGATATA TCTATGGGTG TTTACAGAAA TCAGTTCAGC ATAAGTGGCC TACAAATAAC 
ACCATGAGAA CAAGAGTCGT TAGGCAGAAT GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011242820.1
CDS	366..3041
Translation	MMAAEAGSEEGGPATAGPGGGAATGSSAYSAACRVKLPAALPVAAAPCPGLADADLVAALGGGAASGSGFLGTGPVAGVLGGAALTGSAAAGVAGAAAAGPAGDVAFTKGTLSLPAETLGPGGGFPPLPPPPQLPPLGSGLGTVDEGDSLDGPEYEEEEVAIPLTAPPTNQWYHGKLDRTIAEERLRQAGKSGSYLIRESDRRPGSFVLSFLSQTNVVNHFRIIAMCGDYYIGGRRFSSLSDLIGYYSHVSCLLKGEKLLYPVAPPEPVEDRRRVRAILPYTKVPDTDEISFLKGDMFIVHNELEDGWMWVTNLRTDEQGLIVEDLVEEVGREEDPHEGKIWFHGKISKQEAYNLLMTVGQVCSFLVRPSDNTPGDYSLYFRTNENIQRFKICPTPNNQFMMGGRYYNSIGDIIDHYRKEQIVEGYYLKEPVPMQDQGQVLNDTVDGKEIYNTIRRKTKDAFYKNIVKKGYLLKKGKGKRWKNLYFILEGSDAQLIYFESEKRATKPKGLIDLSVCSVYVVHDSLFGRPNCFQIVVQHFSEEHYIFYFAGETPEQAEDWMKGLQAFCSLRKSSPGTSNKRLRQVSSLVLHIEEAHKLPVKHFTNPYCNIYLNSVQVAKTHAREGQNPVWSEEFVFDDLPPDINRFEITLSNKTKKSKDPDILFMRCQLSRLQKGHATDEWFLLSSHIPLKGIEPGSLRVRARYSMEKIMPEEEYSEFKELILQKELHVVYALSHVCGQDRTLLASILLKIFLHEKLESLLLCTLNDREISMEDEATTLFRATTLASTLMEQYMKATATQFVHHALKDSILKIMESKQSCELSPSKLEKNEDVNTNLAHLLSILSELVEKIFMASEILPPTLRYIYGCLQKSVQHKWPTNNTMRTRVVRQNV

Target ORF information:

RefSeq Version	XM_011244518.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus RAS p21 protein activator 1 (Rasa1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011244518.3

ORF Insert Sequence:

ATGATGGCGG CCGAGGCGGG CAGTGAGGAA GGCGGCCCTG CGACGGCGGG CCCCGGCGGC 
GGCGCGGCCA CGGGCTCCAG CGCCTACTCG GCAGCGTGCC GGGTCAAGTT ACCCGCGGCC 
CTGCCGGTGG CAGCCGCCCC GTGTCCCGGG CTGGCGGACG CCGACTTGGT TGCGGCCCTC 
GGTGGCGGAG CCGCGTCGGG GTCCGGGTTC CTGGGGACCG GGCCGGTGGC CGGTGTCTTG 
GGGGGCGCCG CGCTGACCGG CAGTGCTGCT GCTGGCGTGG CTGGTGCTGC GGCCGCCGGA 
CCTGCGGGAG ACGTCGCTTT CACCAAGGGG ACTCTGTCTT TGCCTGCTGA GACGCTCGGG 
CCGGGCGGCG GATTCCCTCC TCTGCCGCCG CCTCCTCAGC TGCCCCCTTT GGGCTCCGGT 
TTGGGGACCG TGGATGAAGG TGACTCCCTG GATGGACCAG AATACGAAGA GGAAGAGGTG 
GCCATACCGC TGACCGCACC TCCCACTAAC CAGTGGTATC ATGGAAAACT TGATAGAACT 
ATAGCAGAAG AACGCCTCAG GCAGGCAGGG AAATCTGGAA GCTATCTTAT AAGAGAGAGT 
GATCGGAGGC CGGGGTCCTT TGTACTTTCA TTCCTTAGTC AGACAAATGT TGTCAATCAT 
TTTAGGATTA TTGCTATGTG TGGTGATTAC TACATTGGTG GAAGGCGTTT TTCTTCACTC 
TCAGACCTAA TAGGTTATTA CAGTCACGTT TCTTGTTTAC TTAAAGGAGA AAAATTACTC 
TATCCAGTTG CACCACCAGA GCCAGTAGAA GATAGAAGGC GTGTACGAGC CATTCTGCCC 
TACACAAAAG TACCAGACAC TGATGAAATA AGTTTTTTAA AAGGAGATAT GTTCATTGTT 
CACAATGAGT TAGAAGATGG ATGGATGTGG GTTACCAATC TAAGAACAGA TGAGCAAGGC 
CTTATTGTTG AAGACCTAGT GGAAGAGGTG GGCCGAGAAG AAGATCCACA CGAAGGCAAA 
ATATGGTTCC ACGGGAAAAT TTCCAAACAA GAAGCTTATA ATTTATTAAT GACAGTTGGA 
CAAGTCTGCA GCTTTCTTGT GAGGCCCTCA GATAATACTC CTGGAGATTA TTCTCTGTAT 
TTTCGGACCA ATGAAAATAT TCAGCGATTT AAAATTTGTC CAACACCTAA CAACCAGTTT 
ATGATGGGAG GCCGCTATTA TAACAGTATC GGGGACATCA TAGACCATTA TCGGAAAGAA 
CAGATTGTTG AAGGCTATTA TCTTAAGGAA CCTGTACCAA TGCAGGATCA AGGGCAAGTA 
CTGAACGACA CTGTGGATGG GAAGGAGATC TATAACACAA TTCGTCGGAA GACAAAGGAT 
GCTTTTTATA AAAATATTGT TAAAAAAGGT TATCTTCTGA AGAAGGGTAA AGGAAAACGT 
TGGAAAAATT TATATTTTAT CTTAGAAGGC AGTGATGCTC AACTTATTTA TTTTGAAAGT 
GAAAAACGAG CTACAAAACC AAAAGGGCTA ATAGACCTTA GTGTGTGTTC TGTCTACGTT 
GTCCATGACA GTCTCTTCGG CAGGCCAAAC TGTTTTCAGA TAGTAGTTCA GCACTTTAGT 
GAAGAACATT ACATCTTTTA CTTTGCAGGA GAAACTCCAG AACAAGCAGA GGATTGGATG 
AAAGGTCTGC AAGCATTTTG CAGTTTACGG AAAAGTAGCC CAGGGACATC TAATAAACGC 
CTTCGTCAGG TCAGCAGCCT TGTTTTACAT ATTGAAGAAG CCCATAAACT ACCAGTAAAA 
CACTTTACTA ATCCATATTG TAACATCTAC TTGAATAGCG TCCAAGTAGC AAAAACTCAT 
GCAAGGGAGG GGCAAAACCC AGTATGGTCA GAGGAGTTTG TCTTTGATGA TCTTCCTCCT 
GACATCAATA GATTTGAAAT CACACTTAGT AATAAAACAA AGAAAAGCAA AGATCCAGAT 
ATCTTATTTA TGCGTTGCCA GTTGAGCAGG TTACAGAAAG GACATGCAAC AGATGAATGG 
TTTCTACTAA GTTCCCATAT ACCACTAAAA GGTATTGAAC CAGGATCTTT GCGTGTTCGA 
GCACGATACT CCATGGAAAA AATCATGCCA GAAGAAGAGT ACAGTGAATT TAAAGAGCTT 
ATACTGCAAA AGGAACTTCA TGTCGTCTAT GCTTTATCAC ATGTATGTGG ACAAGACCGA 
ACACTACTGG CCAGCATCCT ACTAAAGATA TTTCTTCATG AAAAGCTTGA ATCATTGTTG 
TTATGCACAC TAAATGACAG AGAGATAAGT ATGGAAGATG AAGCCACTAC CCTATTTCGA 
GCTACAACAC TTGCAAGTAC CTTGATGGAG CAGTATATGA AAGCTACTGC CACACAGTTT 
GTTCATCATG CTTTGAAAGA CTCCATTTTA AAGATTATGG AAAGCAAGCA ATCATGTGAG 
TTAAGTCCGT CAAAGTTAGA AAAAAATGAA GATGTGAACA CTAATTTAGC ACACCTATTG 
AGCATACTTT CAGAGCTTGT GGAGAAAATA TTCATGGCTT CAGAAATACT TCCACCGACA 
CTGAGATATA TCTATGGGTG TTTACAGAAA TCAGTTCAGC ATAAGTGGCC TACAAATAAC 
ACCATGAGAA CAAGAGTCGT TAGGCAGAAT GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rasa1 ( NM_145452.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_663427.2 Mus musculus RAS p21 protein activator 1 (Rasa1), mRNA.

Price & Availability↑

CloneID	OMu13108
Clone ID Related Accession (Same CDS sequence)	NM_145452.3
Accession Version	NM_145452.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3117bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-26
Organism	Mus musculus(house mouse)
Product	ras GTPase-activating protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB645489.1, BU055087.1, BC065082.1, CV555618.1 and CF534745.1. On Jan 12, 2008 this sequence version replaced NM_145452.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC065082.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164138, SAMN01164139 [ECO:0000348] ##Evidence-Data-END##

ATGATGGCGG CCGAGGCGGG CAGTGAGGAA GGCGGCCCTG CGACGGCGGG CCCCGGCGGC 
GGCGCGGCCA CGGGCTCCAG CGCCTACTCG GCAGCGTGCC GGGTCAAGTT ACCCGCGGCC 
CTGCCGGTGG CAGCCGCCCC GTGTCCCGGG CTGGCGGACG CCGACTTGGT TGCGGCCCTC 
GGTGGCGGAG CCGCGTCGGG GTCCGGGTTC CTGGGGACCG GGCCGGTGGC CGGTGTCTTG 
GGGGGCGCCG CGCTGACCGG CAGTGCTGCT GCTGGCGTGG CTGGTGCTGC GGCCGCCGGA 
CCTGCGGGAG ACGTCGCTTT CACCAAGGGG ACTCTGTCTT TGCCTGCTGA GACGCTCGGG 
CCGGGCGGCG GATTCCCTCC TCTGCCGCCG CCTCCTCAGC TGCCCCCTTT GGGCTCCGGT 
TTGGGGACCG TGGATGAAGG TGACTCCCTG GATGGACCAG AATACGAAGA GGAAGAGGTG 
GCCATACCGC TGACCGCACC TCCCACTAAC CAGTGGTATC ATGGAAAACT TGATAGAACT 
ATAGCAGAAG AACGCCTCAG GCAGGCAGGG AAATCTGGAA GCTATCTTAT AAGAGAGAGT 
GATCGGAGGC CGGGGTCCTT TGTACTTTCA TTCCTTAGTC AGACAAATGT TGTCAATCAT 
TTTAGGATTA TTGCTATGTG TGGTGATTAC TACATTGGTG GAAGGCGTTT TTCTTCACTC 
TCAGACCTAA TAGGTTATTA CAGTCACGTT TCTTGTTTAC TTAAAGGAGA AAAATTACTC 
TATCCAGTTG CACCACCAGA GCCAGTAGAA GATAGAAGGC GTGTACGAGC CATTCTGCCC 
TACACAAAAG TACCAGACAC TGATGAAATA AGTTTTTTAA AAGGAGATAT GTTCATTGTT 
CACAATGAGT TAGAAGATGG ATGGATGTGG GTTACCAATC TAAGAACAGA TGAGCAAGGC 
CTTATTGTTG AAGACCTAGT GGAAGAGGTG GGCCGAGAAG AAGATCCACA CGAAGGCAAA 
ATATGGTTCC ACGGGAAAAT TTCCAAACAA GAAGCTTATA ATTTATTAAT GACAGTTGGA 
CAAGTCTGCA GCTTTCTTGT GAGGCCCTCA GATAATACTC CTGGAGATTA TTCTCTGTAT 
TTTCGGACCA ATGAAAATAT TCAGCGATTT AAAATTTGTC CAACACCTAA CAACCAGTTT 
ATGATGGGAG GCCGCTATTA TAACAGTATC GGGGACATCA TAGACCATTA TCGGAAAGAA 
CAGATTGTTG AAGGCTATTA TCTTAAGGAA CCTGTACCAA TGCAGGATCA AGGGCAAGTA 
CTGAACGACA CTGTGGATGG GAAGGAGATC TATAACACAA TTCGTCGGAA GACAAAGGAT 
GCTTTTTATA AAAATATTGT TAAAAAAGGT TATCTTCTGA AGAAGGGTAA AGGAAAACGT 
TGGAAAAATT TATATTTTAT CTTAGAAGGC AGTGATGCTC AACTTATTTA TTTTGAAAGT 
GAAAAACGAG CTACAAAACC AAAAGGGCTA ATAGACCTTA GTGTGTGTTC TGTCTACGTT 
GTCCATGACA GTCTCTTCGG CAGGCCAAAC TGTTTTCAGA TAGTAGTTCA GCACTTTAGT 
GAAGAACATT ACATCTTTTA CTTTGCAGGA GAAACTCCAG AACAAGCAGA GGATTGGATG 
AAAGGTCTGC AAGCATTTTG CAGTTTACGG AAAAGTAGCC CAGGGACATC TAATAAACGC 
CTTCGTCAGG TCAGCAGCCT TGTTTTACAT ATTGAAGAAG CCCATAAACT ACCAGTAAAA 
CACTTTACTA ATCCATATTG TAACATCTAC TTGAATAGCG TCCAAGTAGC AAAAACTCAT 
GCAAGGGAGG GGCAAAACCC AGTATGGTCA GAGGAGTTTG TCTTTGATGA TCTTCCTCCT 
GACATCAATA GATTTGAAAT CACACTTAGT AATAAAACAA AGAAAAGCAA AGATCCAGAT 
ATCTTATTTA TGCGTTGCCA GTTGAGCAGG TTACAGAAAG GACATGCAAC AGATGAATGG 
TTTCTACTAA GTTCCCATAT ACCACTAAAA GGTATTGAAC CAGGATCTTT GCGTGTTCGA 
GCACGATACT CCATGGAAAA AATCATGCCA GAAGAAGAGT ACAGTGAATT TAAAGAGCTT 
ATACTGCAAA AGGAACTTCA TGTCGTCTAT GCTTTATCAC ATGTATGTGG ACAAGACCGA 
ACACTACTGG CCAGCATCCT ACTAAAGATA TTTCTTCATG AAAAGCTTGA ATCATTGTTG 
TTATGCACAC TAAATGACAG AGAGATAAGT ATGGAAGATG AAGCCACTAC CCTATTTCGA 
GCTACAACAC TTGCAAGTAC CTTGATGGAG CAGTATATGA AAGCTACTGC CACACAGTTT 
GTTCATCATG CTTTGAAAGA CTCCATTTTA AAGATTATGG AAAGCAAGCA ATCATGTGAG 
TTAAGTCCGT CAAAGTTAGA AAAAAATGAA GATGTGAACA CTAATTTAGC ACACCTATTG 
AGCATACTTT CAGAGCTTGT GGAGAAAATA TTCATGGCTT CAGAAATACT TCCACCGACA 
CTGAGATATA TCTATGGGTG TTTACAGAAA TCAGTTCAGC ATAAGTGGCC TACAAATAAC 
ACCATGAGAA CAAGAGTCGT TAGTGGTTTT GTTTTTCTTC GCCTTATCTG CCCTGCTATC 
CTGAATCCAC GGATGTTTAA TATCATCTCA GATTCCCCAT CTCCTATTGC TGCAAGAACA 
CTGACATTAG TGGCTAAATC TGTGCAGAAT TTAGCAAATC TTGTGGAATT TGGAGCTAAG 
GAGCCTTATA TGGAAGGTGT CAATCCATTT ATAAAAAGTA ATAAACATCG CATGATCATG 
TTTTTAGATG AACTTGGGAA TGTACCTGAA CTTCCGGACA CTACAGAGCA TTCTAGAACT 
GACCTGTCTC GTGACTTAGC AGCATTGCAT GAGATTTGTG TAGCTCATTC AGATGAACTG 
AGAACACTCA GCAATGAGCG TGGTGTACAG CAGCACGTAC TGAAAAAGCT TCTGGCGATA 
ACAGAACTGC TTCAACAAAA ACAAAACCAG TATACAAAAA CCAATGACAT CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_663427.2
CDS	580..3696
Translation	MMAAEAGSEEGGPATAGPGGGAATGSSAYSAACRVKLPAALPVAAAPCPGLADADLVAALGGGAASGSGFLGTGPVAGVLGGAALTGSAAAGVAGAAAAGPAGDVAFTKGTLSLPAETLGPGGGFPPLPPPPQLPPLGSGLGTVDEGDSLDGPEYEEEEVAIPLTAPPTNQWYHGKLDRTIAEERLRQAGKSGSYLIRESDRRPGSFVLSFLSQTNVVNHFRIIAMCGDYYIGGRRFSSLSDLIGYYSHVSCLLKGEKLLYPVAPPEPVEDRRRVRAILPYTKVPDTDEISFLKGDMFIVHNELEDGWMWVTNLRTDEQGLIVEDLVEEVGREEDPHEGKIWFHGKISKQEAYNLLMTVGQVCSFLVRPSDNTPGDYSLYFRTNENIQRFKICPTPNNQFMMGGRYYNSIGDIIDHYRKEQIVEGYYLKEPVPMQDQGQVLNDTVDGKEIYNTIRRKTKDAFYKNIVKKGYLLKKGKGKRWKNLYFILEGSDAQLIYFESEKRATKPKGLIDLSVCSVYVVHDSLFGRPNCFQIVVQHFSEEHYIFYFAGETPEQAEDWMKGLQAFCSLRKSSPGTSNKRLRQVSSLVLHIEEAHKLPVKHFTNPYCNIYLNSVQVAKTHAREGQNPVWSEEFVFDDLPPDINRFEITLSNKTKKSKDPDILFMRCQLSRLQKGHATDEWFLLSSHIPLKGIEPGSLRVRARYSMEKIMPEEEYSEFKELILQKELHVVYALSHVCGQDRTLLASILLKIFLHEKLESLLLCTLNDREISMEDEATTLFRATTLASTLMEQYMKATATQFVHHALKDSILKIMESKQSCELSPSKLEKNEDVNTNLAHLLSILSELVEKIFMASEILPPTLRYIYGCLQKSVQHKWPTNNTMRTRVVSGFVFLRLICPAILNPRMFNIISDSPSPIAARTLTLVAKSVQNLANLVEFGAKEPYMEGVNPFIKSNKHRMIMFLDELGNVPELPDTTEHSRTDLSRDLAALHEICVAHSDELRTLSNERGVQQHVLKKLLAITELLQQKQNQYTKTNDIR

Target ORF information:

RefSeq Version	NM_145452.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus RAS p21 protein activator 1 (Rasa1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145452.3

ORF Insert Sequence:

ATGATGGCGG CCGAGGCGGG CAGTGAGGAA GGCGGCCCTG CGACGGCGGG CCCCGGCGGC 
GGCGCGGCCA CGGGCTCCAG CGCCTACTCG GCAGCGTGCC GGGTCAAGTT ACCCGCGGCC 
CTGCCGGTGG CAGCCGCCCC GTGTCCCGGG CTGGCGGACG CCGACTTGGT TGCGGCCCTC 
GGTGGCGGAG CCGCGTCGGG GTCCGGGTTC CTGGGGACCG GGCCGGTGGC CGGTGTCTTG 
GGGGGCGCCG CGCTGACCGG CAGTGCTGCT GCTGGCGTGG CTGGTGCTGC GGCCGCCGGA 
CCTGCGGGAG ACGTCGCTTT CACCAAGGGG ACTCTGTCTT TGCCTGCTGA GACGCTCGGG 
CCGGGCGGCG GATTCCCTCC TCTGCCGCCG CCTCCTCAGC TGCCCCCTTT GGGCTCCGGT 
TTGGGGACCG TGGATGAAGG TGACTCCCTG GATGGACCAG AATACGAAGA GGAAGAGGTG 
GCCATACCGC TGACCGCACC TCCCACTAAC CAGTGGTATC ATGGAAAACT TGATAGAACT 
ATAGCAGAAG AACGCCTCAG GCAGGCAGGG AAATCTGGAA GCTATCTTAT AAGAGAGAGT 
GATCGGAGGC CGGGGTCCTT TGTACTTTCA TTCCTTAGTC AGACAAATGT TGTCAATCAT 
TTTAGGATTA TTGCTATGTG TGGTGATTAC TACATTGGTG GAAGGCGTTT TTCTTCACTC 
TCAGACCTAA TAGGTTATTA CAGTCACGTT TCTTGTTTAC TTAAAGGAGA AAAATTACTC 
TATCCAGTTG CACCACCAGA GCCAGTAGAA GATAGAAGGC GTGTACGAGC CATTCTGCCC 
TACACAAAAG TACCAGACAC TGATGAAATA AGTTTTTTAA AAGGAGATAT GTTCATTGTT 
CACAATGAGT TAGAAGATGG ATGGATGTGG GTTACCAATC TAAGAACAGA TGAGCAAGGC 
CTTATTGTTG AAGACCTAGT GGAAGAGGTG GGCCGAGAAG AAGATCCACA CGAAGGCAAA 
ATATGGTTCC ACGGGAAAAT TTCCAAACAA GAAGCTTATA ATTTATTAAT GACAGTTGGA 
CAAGTCTGCA GCTTTCTTGT GAGGCCCTCA GATAATACTC CTGGAGATTA TTCTCTGTAT 
TTTCGGACCA ATGAAAATAT TCAGCGATTT AAAATTTGTC CAACACCTAA CAACCAGTTT 
ATGATGGGAG GCCGCTATTA TAACAGTATC GGGGACATCA TAGACCATTA TCGGAAAGAA 
CAGATTGTTG AAGGCTATTA TCTTAAGGAA CCTGTACCAA TGCAGGATCA AGGGCAAGTA 
CTGAACGACA CTGTGGATGG GAAGGAGATC TATAACACAA TTCGTCGGAA GACAAAGGAT 
GCTTTTTATA AAAATATTGT TAAAAAAGGT TATCTTCTGA AGAAGGGTAA AGGAAAACGT 
TGGAAAAATT TATATTTTAT CTTAGAAGGC AGTGATGCTC AACTTATTTA TTTTGAAAGT 
GAAAAACGAG CTACAAAACC AAAAGGGCTA ATAGACCTTA GTGTGTGTTC TGTCTACGTT 
GTCCATGACA GTCTCTTCGG CAGGCCAAAC TGTTTTCAGA TAGTAGTTCA GCACTTTAGT 
GAAGAACATT ACATCTTTTA CTTTGCAGGA GAAACTCCAG AACAAGCAGA GGATTGGATG 
AAAGGTCTGC AAGCATTTTG CAGTTTACGG AAAAGTAGCC CAGGGACATC TAATAAACGC 
CTTCGTCAGG TCAGCAGCCT TGTTTTACAT ATTGAAGAAG CCCATAAACT ACCAGTAAAA 
CACTTTACTA ATCCATATTG TAACATCTAC TTGAATAGCG TCCAAGTAGC AAAAACTCAT 
GCAAGGGAGG GGCAAAACCC AGTATGGTCA GAGGAGTTTG TCTTTGATGA TCTTCCTCCT 
GACATCAATA GATTTGAAAT CACACTTAGT AATAAAACAA AGAAAAGCAA AGATCCAGAT 
ATCTTATTTA TGCGTTGCCA GTTGAGCAGG TTACAGAAAG GACATGCAAC AGATGAATGG 
TTTCTACTAA GTTCCCATAT ACCACTAAAA GGTATTGAAC CAGGATCTTT GCGTGTTCGA 
GCACGATACT CCATGGAAAA AATCATGCCA GAAGAAGAGT ACAGTGAATT TAAAGAGCTT 
ATACTGCAAA AGGAACTTCA TGTCGTCTAT GCTTTATCAC ATGTATGTGG ACAAGACCGA 
ACACTACTGG CCAGCATCCT ACTAAAGATA TTTCTTCATG AAAAGCTTGA ATCATTGTTG 
TTATGCACAC TAAATGACAG AGAGATAAGT ATGGAAGATG AAGCCACTAC CCTATTTCGA 
GCTACAACAC TTGCAAGTAC CTTGATGGAG CAGTATATGA AAGCTACTGC CACACAGTTT 
GTTCATCATG CTTTGAAAGA CTCCATTTTA AAGATTATGG AAAGCAAGCA ATCATGTGAG 
TTAAGTCCGT CAAAGTTAGA AAAAAATGAA GATGTGAACA CTAATTTAGC ACACCTATTG 
AGCATACTTT CAGAGCTTGT GGAGAAAATA TTCATGGCTT CAGAAATACT TCCACCGACA 
CTGAGATATA TCTATGGGTG TTTACAGAAA TCAGTTCAGC ATAAGTGGCC TACAAATAAC 
ACCATGAGAA CAAGAGTCGT TAGTGGTTTT GTTTTTCTTC GCCTTATCTG CCCTGCTATC 
CTGAATCCAC GGATGTTTAA TATCATCTCA GATTCCCCAT CTCCTATTGC TGCAAGAACA 
CTGACATTAG TGGCTAAATC TGTGCAGAAT TTAGCAAATC TTGTGGAATT TGGAGCTAAG 
GAGCCTTATA TGGAAGGTGT CAATCCATTT ATAAAAAGTA ATAAACATCG CATGATCATG 
TTTTTAGATG AACTTGGGAA TGTACCTGAA CTTCCGGACA CTACAGAGCA TTCTAGAACT 
GACCTGTCTC GTGACTTAGC AGCATTGCAT GAGATTTGTG TAGCTCATTC AGATGAACTG 
AGAACACTCA GCAATGAGCG TGGTGTACAG CAGCACGTAC TGAAAAAGCT TCTGGCGATA 
ACAGAACTGC TTCAACAAAA ACAAAACCAG TATACAAAAA CCAATGACAT CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rasa1 ( XM_011244518.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011242820.1 Mus musculus RAS p21 protein activator 1 (Rasa1), transcript variant X1, mRNA.

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CloneID	OMu73058
Clone ID Related Accession (Same CDS sequence)	XM_011244518.2 , XM_011244518.3
Accession Version	XM_011244518.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2676bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ras GTPase-activating protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011244518.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCGG CCGAGGCGGG CAGTGAGGAA GGCGGCCCTG CGACGGCGGG CCCCGGCGGC 
GGCGCGGCCA CGGGCTCCAG CGCCTACTCG GCAGCGTGCC GGGTCAAGTT ACCCGCGGCC 
CTGCCGGTGG CAGCCGCCCC GTGTCCCGGG CTGGCGGACG CCGACTTGGT TGCGGCCCTC 
GGTGGCGGAG CCGCGTCGGG GTCCGGGTTC CTGGGGACCG GGCCGGTGGC CGGTGTCTTG 
GGGGGCGCCG CGCTGACCGG CAGTGCTGCT GCTGGCGTGG CTGGTGCTGC GGCCGCCGGA 
CCTGCGGGAG ACGTCGCTTT CACCAAGGGG ACTCTGTCTT TGCCTGCTGA GACGCTCGGG 
CCGGGCGGCG GATTCCCTCC TCTGCCGCCG CCTCCTCAGC TGCCCCCTTT GGGCTCCGGT 
TTGGGGACCG TGGATGAAGG TGACTCCCTG GATGGACCAG AATACGAAGA GGAAGAGGTG 
GCCATACCGC TGACCGCACC TCCCACTAAC CAGTGGTATC ATGGAAAACT TGATAGAACT 
ATAGCAGAAG AACGCCTCAG GCAGGCAGGG AAATCTGGAA GCTATCTTAT AAGAGAGAGT 
GATCGGAGGC CGGGGTCCTT TGTACTTTCA TTCCTTAGTC AGACAAATGT TGTCAATCAT 
TTTAGGATTA TTGCTATGTG TGGTGATTAC TACATTGGTG GAAGGCGTTT TTCTTCACTC 
TCAGACCTAA TAGGTTATTA CAGTCACGTT TCTTGTTTAC TTAAAGGAGA AAAATTACTC 
TATCCAGTTG CACCACCAGA GCCAGTAGAA GATAGAAGGC GTGTACGAGC CATTCTGCCC 
TACACAAAAG TACCAGACAC TGATGAAATA AGTTTTTTAA AAGGAGATAT GTTCATTGTT 
CACAATGAGT TAGAAGATGG ATGGATGTGG GTTACCAATC TAAGAACAGA TGAGCAAGGC 
CTTATTGTTG AAGACCTAGT GGAAGAGGTG GGCCGAGAAG AAGATCCACA CGAAGGCAAA 
ATATGGTTCC ACGGGAAAAT TTCCAAACAA GAAGCTTATA ATTTATTAAT GACAGTTGGA 
CAAGTCTGCA GCTTTCTTGT GAGGCCCTCA GATAATACTC CTGGAGATTA TTCTCTGTAT 
TTTCGGACCA ATGAAAATAT TCAGCGATTT AAAATTTGTC CAACACCTAA CAACCAGTTT 
ATGATGGGAG GCCGCTATTA TAACAGTATC GGGGACATCA TAGACCATTA TCGGAAAGAA 
CAGATTGTTG AAGGCTATTA TCTTAAGGAA CCTGTACCAA TGCAGGATCA AGGGCAAGTA 
CTGAACGACA CTGTGGATGG GAAGGAGATC TATAACACAA TTCGTCGGAA GACAAAGGAT 
GCTTTTTATA AAAATATTGT TAAAAAAGGT TATCTTCTGA AGAAGGGTAA AGGAAAACGT 
TGGAAAAATT TATATTTTAT CTTAGAAGGC AGTGATGCTC AACTTATTTA TTTTGAAAGT 
GAAAAACGAG CTACAAAACC AAAAGGGCTA ATAGACCTTA GTGTGTGTTC TGTCTACGTT 
GTCCATGACA GTCTCTTCGG CAGGCCAAAC TGTTTTCAGA TAGTAGTTCA GCACTTTAGT 
GAAGAACATT ACATCTTTTA CTTTGCAGGA GAAACTCCAG AACAAGCAGA GGATTGGATG 
AAAGGTCTGC AAGCATTTTG CAGTTTACGG AAAAGTAGCC CAGGGACATC TAATAAACGC 
CTTCGTCAGG TCAGCAGCCT TGTTTTACAT ATTGAAGAAG CCCATAAACT ACCAGTAAAA 
CACTTTACTA ATCCATATTG TAACATCTAC TTGAATAGCG TCCAAGTAGC AAAAACTCAT 
GCAAGGGAGG GGCAAAACCC AGTATGGTCA GAGGAGTTTG TCTTTGATGA TCTTCCTCCT 
GACATCAATA GATTTGAAAT CACACTTAGT AATAAAACAA AGAAAAGCAA AGATCCAGAT 
ATCTTATTTA TGCGTTGCCA GTTGAGCAGG TTACAGAAAG GACATGCAAC AGATGAATGG 
TTTCTACTAA GTTCCCATAT ACCACTAAAA GGTATTGAAC CAGGATCTTT GCGTGTTCGA 
GCACGATACT CCATGGAAAA AATCATGCCA GAAGAAGAGT ACAGTGAATT TAAAGAGCTT 
ATACTGCAAA AGGAACTTCA TGTCGTCTAT GCTTTATCAC ATGTATGTGG ACAAGACCGA 
ACACTACTGG CCAGCATCCT ACTAAAGATA TTTCTTCATG AAAAGCTTGA ATCATTGTTG 
TTATGCACAC TAAATGACAG AGAGATAAGT ATGGAAGATG AAGCCACTAC CCTATTTCGA 
GCTACAACAC TTGCAAGTAC CTTGATGGAG CAGTATATGA AAGCTACTGC CACACAGTTT 
GTTCATCATG CTTTGAAAGA CTCCATTTTA AAGATTATGG AAAGCAAGCA ATCATGTGAG 
TTAAGTCCGT CAAAGTTAGA AAAAAATGAA GATGTGAACA CTAATTTAGC ACACCTATTG 
AGCATACTTT CAGAGCTTGT GGAGAAAATA TTCATGGCTT CAGAAATACT TCCACCGACA 
CTGAGATATA TCTATGGGTG TTTACAGAAA TCAGTTCAGC ATAAGTGGCC TACAAATAAC 
ACCATGAGAA CAAGAGTCGT TAGGCAGAAT GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011242820.1
CDS	587..3262
Translation	MMAAEAGSEEGGPATAGPGGGAATGSSAYSAACRVKLPAALPVAAAPCPGLADADLVAALGGGAASGSGFLGTGPVAGVLGGAALTGSAAAGVAGAAAAGPAGDVAFTKGTLSLPAETLGPGGGFPPLPPPPQLPPLGSGLGTVDEGDSLDGPEYEEEEVAIPLTAPPTNQWYHGKLDRTIAEERLRQAGKSGSYLIRESDRRPGSFVLSFLSQTNVVNHFRIIAMCGDYYIGGRRFSSLSDLIGYYSHVSCLLKGEKLLYPVAPPEPVEDRRRVRAILPYTKVPDTDEISFLKGDMFIVHNELEDGWMWVTNLRTDEQGLIVEDLVEEVGREEDPHEGKIWFHGKISKQEAYNLLMTVGQVCSFLVRPSDNTPGDYSLYFRTNENIQRFKICPTPNNQFMMGGRYYNSIGDIIDHYRKEQIVEGYYLKEPVPMQDQGQVLNDTVDGKEIYNTIRRKTKDAFYKNIVKKGYLLKKGKGKRWKNLYFILEGSDAQLIYFESEKRATKPKGLIDLSVCSVYVVHDSLFGRPNCFQIVVQHFSEEHYIFYFAGETPEQAEDWMKGLQAFCSLRKSSPGTSNKRLRQVSSLVLHIEEAHKLPVKHFTNPYCNIYLNSVQVAKTHAREGQNPVWSEEFVFDDLPPDINRFEITLSNKTKKSKDPDILFMRCQLSRLQKGHATDEWFLLSSHIPLKGIEPGSLRVRARYSMEKIMPEEEYSEFKELILQKELHVVYALSHVCGQDRTLLASILLKIFLHEKLESLLLCTLNDREISMEDEATTLFRATTLASTLMEQYMKATATQFVHHALKDSILKIMESKQSCELSPSKLEKNEDVNTNLAHLLSILSELVEKIFMASEILPPTLRYIYGCLQKSVQHKWPTNNTMRTRVVRQNV

Target ORF information:

RefSeq Version	XM_011244518.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus RAS p21 protein activator 1 (Rasa1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011244518.2

ORF Insert Sequence:

ATGATGGCGG CCGAGGCGGG CAGTGAGGAA GGCGGCCCTG CGACGGCGGG CCCCGGCGGC 
GGCGCGGCCA CGGGCTCCAG CGCCTACTCG GCAGCGTGCC GGGTCAAGTT ACCCGCGGCC 
CTGCCGGTGG CAGCCGCCCC GTGTCCCGGG CTGGCGGACG CCGACTTGGT TGCGGCCCTC 
GGTGGCGGAG CCGCGTCGGG GTCCGGGTTC CTGGGGACCG GGCCGGTGGC CGGTGTCTTG 
GGGGGCGCCG CGCTGACCGG CAGTGCTGCT GCTGGCGTGG CTGGTGCTGC GGCCGCCGGA 
CCTGCGGGAG ACGTCGCTTT CACCAAGGGG ACTCTGTCTT TGCCTGCTGA GACGCTCGGG 
CCGGGCGGCG GATTCCCTCC TCTGCCGCCG CCTCCTCAGC TGCCCCCTTT GGGCTCCGGT 
TTGGGGACCG TGGATGAAGG TGACTCCCTG GATGGACCAG AATACGAAGA GGAAGAGGTG 
GCCATACCGC TGACCGCACC TCCCACTAAC CAGTGGTATC ATGGAAAACT TGATAGAACT 
ATAGCAGAAG AACGCCTCAG GCAGGCAGGG AAATCTGGAA GCTATCTTAT AAGAGAGAGT 
GATCGGAGGC CGGGGTCCTT TGTACTTTCA TTCCTTAGTC AGACAAATGT TGTCAATCAT 
TTTAGGATTA TTGCTATGTG TGGTGATTAC TACATTGGTG GAAGGCGTTT TTCTTCACTC 
TCAGACCTAA TAGGTTATTA CAGTCACGTT TCTTGTTTAC TTAAAGGAGA AAAATTACTC 
TATCCAGTTG CACCACCAGA GCCAGTAGAA GATAGAAGGC GTGTACGAGC CATTCTGCCC 
TACACAAAAG TACCAGACAC TGATGAAATA AGTTTTTTAA AAGGAGATAT GTTCATTGTT 
CACAATGAGT TAGAAGATGG ATGGATGTGG GTTACCAATC TAAGAACAGA TGAGCAAGGC 
CTTATTGTTG AAGACCTAGT GGAAGAGGTG GGCCGAGAAG AAGATCCACA CGAAGGCAAA 
ATATGGTTCC ACGGGAAAAT TTCCAAACAA GAAGCTTATA ATTTATTAAT GACAGTTGGA 
CAAGTCTGCA GCTTTCTTGT GAGGCCCTCA GATAATACTC CTGGAGATTA TTCTCTGTAT 
TTTCGGACCA ATGAAAATAT TCAGCGATTT AAAATTTGTC CAACACCTAA CAACCAGTTT 
ATGATGGGAG GCCGCTATTA TAACAGTATC GGGGACATCA TAGACCATTA TCGGAAAGAA 
CAGATTGTTG AAGGCTATTA TCTTAAGGAA CCTGTACCAA TGCAGGATCA AGGGCAAGTA 
CTGAACGACA CTGTGGATGG GAAGGAGATC TATAACACAA TTCGTCGGAA GACAAAGGAT 
GCTTTTTATA AAAATATTGT TAAAAAAGGT TATCTTCTGA AGAAGGGTAA AGGAAAACGT 
TGGAAAAATT TATATTTTAT CTTAGAAGGC AGTGATGCTC AACTTATTTA TTTTGAAAGT 
GAAAAACGAG CTACAAAACC AAAAGGGCTA ATAGACCTTA GTGTGTGTTC TGTCTACGTT 
GTCCATGACA GTCTCTTCGG CAGGCCAAAC TGTTTTCAGA TAGTAGTTCA GCACTTTAGT 
GAAGAACATT ACATCTTTTA CTTTGCAGGA GAAACTCCAG AACAAGCAGA GGATTGGATG 
AAAGGTCTGC AAGCATTTTG CAGTTTACGG AAAAGTAGCC CAGGGACATC TAATAAACGC 
CTTCGTCAGG TCAGCAGCCT TGTTTTACAT ATTGAAGAAG CCCATAAACT ACCAGTAAAA 
CACTTTACTA ATCCATATTG TAACATCTAC TTGAATAGCG TCCAAGTAGC AAAAACTCAT 
GCAAGGGAGG GGCAAAACCC AGTATGGTCA GAGGAGTTTG TCTTTGATGA TCTTCCTCCT 
GACATCAATA GATTTGAAAT CACACTTAGT AATAAAACAA AGAAAAGCAA AGATCCAGAT 
ATCTTATTTA TGCGTTGCCA GTTGAGCAGG TTACAGAAAG GACATGCAAC AGATGAATGG 
TTTCTACTAA GTTCCCATAT ACCACTAAAA GGTATTGAAC CAGGATCTTT GCGTGTTCGA 
GCACGATACT CCATGGAAAA AATCATGCCA GAAGAAGAGT ACAGTGAATT TAAAGAGCTT 
ATACTGCAAA AGGAACTTCA TGTCGTCTAT GCTTTATCAC ATGTATGTGG ACAAGACCGA 
ACACTACTGG CCAGCATCCT ACTAAAGATA TTTCTTCATG AAAAGCTTGA ATCATTGTTG 
TTATGCACAC TAAATGACAG AGAGATAAGT ATGGAAGATG AAGCCACTAC CCTATTTCGA 
GCTACAACAC TTGCAAGTAC CTTGATGGAG CAGTATATGA AAGCTACTGC CACACAGTTT 
GTTCATCATG CTTTGAAAGA CTCCATTTTA AAGATTATGG AAAGCAAGCA ATCATGTGAG 
TTAAGTCCGT CAAAGTTAGA AAAAAATGAA GATGTGAACA CTAATTTAGC ACACCTATTG 
AGCATACTTT CAGAGCTTGT GGAGAAAATA TTCATGGCTT CAGAAATACT TCCACCGACA 
CTGAGATATA TCTATGGGTG TTTACAGAAA TCAGTTCAGC ATAAGTGGCC TACAAATAAC 
ACCATGAGAA CAAGAGTCGT TAGGCAGAAT GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RASA1 regulates the function of lymphatic vessel valves in mice.
The Journal of clinical investigation127(7)2569-2585(2017 Jun)
Lapinski PE,Lubeck BA,Chen D,Doosti A,Zawieja SD,Davis MJ,King PD

The caspase-3/p120 RasGAP stress-sensing module reduces liver cancer incidence but does not affect overall survival in gamma-irradiated and carcinogen-treated mice.
Molecular carcinogenesis56(6)1680-1684(2017 Jun)
Vanli G,Sempoux C,Widmann C

The caspase-3-p120-RasGAP module generates a NF-?B repressor in response to cellular stress.
Journal of cell science128(18)3502-13(2015 Sep)
Khalil H,Loukili N,Regamey A,Cuesta-Marban A,Santori E,Huber M,Widmann C

Cutting Edge: Codeletion of the Ras GTPase-Activating Proteins (RasGAPs) Neurofibromin 1 and p120 RasGAP in T Cells Results in the Development of T Cell Acute Lymphoblastic Leukemia.
Journal of immunology (Baltimore, Md. : 1950)195(1)31-5(2015 Jul)
Lubeck BA,Lapinski PE,Oliver JA,Ksionda O,Parada LF,Zhu Y,Maillard I,Chiang M,Roose J,King PD

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Rasa1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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