Amph cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Amph
Entrez Gene ID	218038
Full Name	amphiphysin
General protein information	Preferred Names amphiphysin Names amphiphysin
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 13 Map Location: 13 A2\|13 6.89 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001289546.1	NP_001276475.1	amphiphysin isoform 2
XM_011244304.3	XP_011242606.1	amphiphysin isoform X1
XM_011244304.2	XP_011242606.1	amphiphysin isoform X1
NM_175007.2	NP_778172.1	amphiphysin isoform 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	AMPH	XP_003318313.1
Rattus norvegicus (Norway rat)	Amph	NP_071553.1
Rattus norvegicus (Norway rat)	LOC100910792	XP_006254144.1
Caenorhabditis elegans (roundworm)	amph-1	NP_501711.1
Xenopus tropicalis (tropical clawed frog)	amph	NP_001096507.1
Homo sapiens (human)	AMPH	NP_001626.1
Canis lupus familiaris (dog)	AMPH	XP_533085.3
Mus musculus (house mouse)	Amph	NP_778172.1
Danio rerio (zebrafish)	amph	NP_957125.1
Gallus gallus (chicken)	AMPH	NP_001004398.1
	AMPH	XP_001100126.1
Bos taurus (cattle)	AMPH	NP_001091599.1

Gene Ontology
Bibliography

Related articles in PubMed

EHBP1L1 coordinates Rab8 and Bin1 to regulate apical-directed transport in polarized epithelial cells.
Nakajo A, Yoshimura S, Togawa H, Kunii M, Iwano T, Izumi A, Noguchi Y, Watanabe A, Goto A, Sato T, Harada A
The Journal of cell biology212(3)297-306(2016 Feb)

Exocytosis regulates trafficking of GABA and glycine heterotransporters in spinal cord glutamatergic synapses: a mechanism for the excessive heterotransporter-induced release of glutamate in experimental amyotrophic lateral sclerosis.
Milanese M, Bonifacino T, Fedele E, Rebosio C, Cattaneo L, Benfenati F, Usai C, Bonanno G
Neurobiology of disease74314-24(2015 Feb)

Stromally expressed ?-catenin modulates Wnt9b signaling in the ureteric epithelium.
Boivin FJ, Sarin S, Lim J, Javidan A, Svajger B, Khalili H, Bridgewater D
PloS one10(3)e0120347(2015)

Mdm2 is required for maintenance of the nephrogenic niche.
Hilliard SA, Yao X, El-Dahr SS
Developmental biology387(1)1-14(2014 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that dynamin, clathrin, adaptor protein complex-2 (AP2), and amphiphysin contribute to the depolarization-evoked entry in endocytosis.
Title: Molecular components required for resting and stimulated endocytosis of botulinum neurotoxins by glutamatergic and peptidergic neurons.

Amph1 is the cytoplasmic substrate for CDKL5 and that its phosphorylation may play crucial roles in the neuronal development.
Title: Identification of amphiphysin 1 as an endogenous substrate for CDKL5, a protein kinase associated with X-linked neurodevelopmental disorder.

amphiphysin interaction with N-WASP regulates actin assembly
Title: Dynamic interaction of amphiphysin with N-WASP regulates actin assembly.

As amphiphysin is involved in the regulation of endocytosis, phosphorylation at this site may play a role in the regulated re-uptake of synaptic vesicles after neurotransmitter release.
Title: Amphiphysin I phosphorylation on residue threonine 260 in a pentylenetetrazole-induced seizure model.

amphiphysin 1 is important in the regulation of actin dynamics
Title: Amphiphysin 1 is important for actin polymerization during phagocytosis.

The following Amph gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Amph cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu20148	NM_001289546.1 Latest version!	Mus musculus amphiphysin (Amph), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu20214	NM_175007.2 Latest version!	Mus musculus amphiphysin (Amph), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OMu73047	XM_011244304.3 Latest version!	Mus musculus amphiphysin (Amph), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu73047	XM_011244304.2	Mus musculus amphiphysin (Amph), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Amph ( XM_011244304.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011242606.1 Mus musculus amphiphysin (Amph), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu73047
Clone ID Related Accession (Same CDS sequence)	XM_011244304.3 , XM_011244304.2
Accession Version	XM_011244304.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	amphiphysin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011244304.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGACA TCAAGACGGG CATCTTCGCC AAAAACGTGC AAAAGCGCCT CAACCGCGCG 
CAGGAAAAGG TCCTCCAGAA GCTGGGGAAG GCTGACGAGA CAAAAGATGA ACAGTTTGAA 
GAATATGTCC AGAACTTCAA ACGGCAGGAG GCAGAGGGTA CCAGACTTCA GCGAGAACTC 
CGGGGATATT TAGCAGCAAT CAAAGGTATG CAGGAGGCCT CAATGAAGCT CACTGAATCT 
CTACATGAAG TCTACGAGCC AGACTGGTAT GGACGGGAAG ATGTAAAGAT GGTTGGTGAG 
AAGTGTGATG TGCTGTGGGA AGACTTCCAT CAAAAGCTAG TGGATGGCTC CTTGCTGACA 
CTGGACACCT ACCTGGGCCA GTTTCCTGAC ATAAAGAATC GCATCGCTAA GCGCAGCAGG 
AAGCTTGTGG ATTATGATAG TGCTCGCCAT CACCTGGAGG CTCTGCAGAG TTCCAAACGG 
AAAGATGAGA GTCGGATCTC TAAGGCAGAA GAGGAGTTTC AGAAAGCACA GAAAGTGTTT 
GAAGAGTTCA ATGTTGACTT ACAAGAAGAG TTGCCGTCAT TGTGGTCAAG TCGAGTTGGC 
TTTTACGTCA ATACTTTCAA AAATGTCTCC AGCCTTGAGG CCAAGTTTCA TAAGGAAATC 
GCAGTGCTTT GCCACAAGCT CTATGAAGTG ATGACGAAAC TCGGTGATCA GCATGCTGAC 
AAGGCCTTCA GCATTCAAGG AGCTCCCAGT GACTCGGGTC CTCTTCGCAT TGCAAAGACA 
CCATCACCTC CAGAGGAGCC TTCTCCCCTG CCCAGCCCCA CGGCTAGTCC CAACCACACA 
TTAGCACCTG CATCTCCTGC CCCAGTGCGA CCCAGATCAC CTTCACAGAC AAGGAAAGGG 
CCTCCTGTCC CACCTCTGCC TAAAGTCACC CCAACAAAGG AACTGAAACA GGAGAACATC 
ATCAATTTCT TTGAGGACAA CTTTGTACCA GAAATCAATG TGACAACACC TTCCCAGAAC 
GAAGTCTTGG AGGTGAAGAA GGAAGAGACT TTGCTGGATT TGGACTTTGA CCCTTTCAAG 
CCCGATGTGG CTCCGGCAGG TTCTGCTGCA GCGACCCACT CTCCCATGTC TCAGACATTG 
CCCTGGGACT TATGGACGAC AAGCACTGAT TTGGTACAAC CAGCTTCTGG GGGTTCATTT 
AATGATTTCA CCCAGGCCCA GGACACTTCA TTATTCACCA TGCAGACAGA TCAGAATATG 
ATCCCTCCCG CAACTGATAC TGGCGAGACT GTGGAAACTG CAGTGCCAAC TGAGGGTGCC 
CCGGTGGAGG AAGCAGAGGC AGAGAAGGCA GCCCTCCCTG CTGGAGAAGG AGGAAGTCCA 
GAGGGAGCCA AAATTGACGG GGAAAGCACG GAGCTTGCAA TCAGTGAGAG CCCTCAACCA 
GTGGAGCCCG AAGCGGGTGC TCCTCAGGTC ATCCCTTCTG TTGTCATTGA GCCAGCCTCC 
AACCATGAAG GAGAAGGAGA ACACCAAGAA ACTGCTACGG GTACTGAACC GAGGGAGGCC 
GCTGAGGATG TGGCTGCCCA GGGCTCAGCG GGTGAGAAGC AGGAAGTGGC TACAGAGCCC 
ACGCCTCTGG ATTCTCAGGC AACTCTGCCT GCATCTGCAG GTGCTGTGGA TGCCTCGCTG 
TCTGCAGGGG ATGCCACTCA GGAACTGCCT CCTGGCTTTC TCTACAAGGT GGAAACCCTG 
CATGATTTCG AGGCAGCGAA CTCTGATGAA CTTAACCTAC AAAGGGGTGA CGTGGTACTC 
GTGGTGCCTT CAGACTCAGA AGCCGACCAG GATGCAGGCT GGTTGGTGGG GGTGAAGGAG 
TCAGACTGGC TTCAGTACAG AGACCTGGCC ACGTACAAAG GCCTCTTTCC AGAGAACTTC 
ACGCGGCGCC TGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011242606.1
CDS	297..2234
Translation	MADIKTGIFAKNVQKRLNRAQEKVLQKLGKADETKDEQFEEYVQNFKRQEAEGTRLQRELRGYLAAIKGMQEASMKLTESLHEVYEPDWYGREDVKMVGEKCDVLWEDFHQKLVDGSLLTLDTYLGQFPDIKNRIAKRSRKLVDYDSARHHLEALQSSKRKDESRISKAEEEFQKAQKVFEEFNVDLQEELPSLWSSRVGFYVNTFKNVSSLEAKFHKEIAVLCHKLYEVMTKLGDQHADKAFSIQGAPSDSGPLRIAKTPSPPEEPSPLPSPTASPNHTLAPASPAPVRPRSPSQTRKGPPVPPLPKVTPTKELKQENIINFFEDNFVPEINVTTPSQNEVLEVKKEETLLDLDFDPFKPDVAPAGSAAATHSPMSQTLPWDLWTTSTDLVQPASGGSFNDFTQAQDTSLFTMQTDQNMIPPATDTGETVETAVPTEGAPVEEAEAEKAALPAGEGGSPEGAKIDGESTELAISESPQPVEPEAGAPQVIPSVVIEPASNHEGEGEHQETATGTEPREAAEDVAAQGSAGEKQEVATEPTPLDSQATLPASAGAVDASLSAGDATQELPPGFLYKVETLHDFEAANSDELNLQRGDVVLVVPSDSEADQDAGWLVGVKESDWLQYRDLATYKGLFPENFTRRLE

Target ORF information:

RefSeq Version	XM_011244304.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus amphiphysin (Amph), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011244304.2

ORF Insert Sequence:

ATGGCCGACA TCAAGACGGG CATCTTCGCC AAAAACGTGC AAAAGCGCCT CAACCGCGCG 
CAGGAAAAGG TCCTCCAGAA GCTGGGGAAG GCTGACGAGA CAAAAGATGA ACAGTTTGAA 
GAATATGTCC AGAACTTCAA ACGGCAGGAG GCAGAGGGTA CCAGACTTCA GCGAGAACTC 
CGGGGATATT TAGCAGCAAT CAAAGGTATG CAGGAGGCCT CAATGAAGCT CACTGAATCT 
CTACATGAAG TCTACGAGCC AGACTGGTAT GGACGGGAAG ATGTAAAGAT GGTTGGTGAG 
AAGTGTGATG TGCTGTGGGA AGACTTCCAT CAAAAGCTAG TGGATGGCTC CTTGCTGACA 
CTGGACACCT ACCTGGGCCA GTTTCCTGAC ATAAAGAATC GCATCGCTAA GCGCAGCAGG 
AAGCTTGTGG ATTATGATAG TGCTCGCCAT CACCTGGAGG CTCTGCAGAG TTCCAAACGG 
AAAGATGAGA GTCGGATCTC TAAGGCAGAA GAGGAGTTTC AGAAAGCACA GAAAGTGTTT 
GAAGAGTTCA ATGTTGACTT ACAAGAAGAG TTGCCGTCAT TGTGGTCAAG TCGAGTTGGC 
TTTTACGTCA ATACTTTCAA AAATGTCTCC AGCCTTGAGG CCAAGTTTCA TAAGGAAATC 
GCAGTGCTTT GCCACAAGCT CTATGAAGTG ATGACGAAAC TCGGTGATCA GCATGCTGAC 
AAGGCCTTCA GCATTCAAGG AGCTCCCAGT GACTCGGGTC CTCTTCGCAT TGCAAAGACA 
CCATCACCTC CAGAGGAGCC TTCTCCCCTG CCCAGCCCCA CGGCTAGTCC CAACCACACA 
TTAGCACCTG CATCTCCTGC CCCAGTGCGA CCCAGATCAC CTTCACAGAC AAGGAAAGGG 
CCTCCTGTCC CACCTCTGCC TAAAGTCACC CCAACAAAGG AACTGAAACA GGAGAACATC 
ATCAATTTCT TTGAGGACAA CTTTGTACCA GAAATCAATG TGACAACACC TTCCCAGAAC 
GAAGTCTTGG AGGTGAAGAA GGAAGAGACT TTGCTGGATT TGGACTTTGA CCCTTTCAAG 
CCCGATGTGG CTCCGGCAGG TTCTGCTGCA GCGACCCACT CTCCCATGTC TCAGACATTG 
CCCTGGGACT TATGGACGAC AAGCACTGAT TTGGTACAAC CAGCTTCTGG GGGTTCATTT 
AATGATTTCA CCCAGGCCCA GGACACTTCA TTATTCACCA TGCAGACAGA TCAGAATATG 
ATCCCTCCCG CAACTGATAC TGGCGAGACT GTGGAAACTG CAGTGCCAAC TGAGGGTGCC 
CCGGTGGAGG AAGCAGAGGC AGAGAAGGCA GCCCTCCCTG CTGGAGAAGG AGGAAGTCCA 
GAGGGAGCCA AAATTGACGG GGAAAGCACG GAGCTTGCAA TCAGTGAGAG CCCTCAACCA 
GTGGAGCCCG AAGCGGGTGC TCCTCAGGTC ATCCCTTCTG TTGTCATTGA GCCAGCCTCC 
AACCATGAAG GAGAAGGAGA ACACCAAGAA ACTGCTACGG GTACTGAACC GAGGGAGGCC 
GCTGAGGATG TGGCTGCCCA GGGCTCAGCG GGTGAGAAGC AGGAAGTGGC TACAGAGCCC 
ACGCCTCTGG ATTCTCAGGC AACTCTGCCT GCATCTGCAG GTGCTGTGGA TGCCTCGCTG 
TCTGCAGGGG ATGCCACTCA GGAACTGCCT CCTGGCTTTC TCTACAAGGT GGAAACCCTG 
CATGATTTCG AGGCAGCGAA CTCTGATGAA CTTAACCTAC AAAGGGGTGA CGTGGTACTC 
GTGGTGCCTT CAGACTCAGA AGCCGACCAG GATGCAGGCT GGTTGGTGGG GGTGAAGGAG 
TCAGACTGGC TTCAGTACAG AGACCTGGCC ACGTACAAAG GCCTCTTTCC AGAGAACTTC 
ACGCGGCGCC TGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Amph ( NM_001289546.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001276475.1 Mus musculus amphiphysin (Amph), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu20148
Clone ID Related Accession (Same CDS sequence)	NM_001289546.1
Accession Version	NM_001289546.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Mus musculus(house mouse)
Product	amphiphysin isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK132168.1, BC054718.1 and BE949100.1. On Jan 23, 2014 this sequence version replaced XM_006516644.1. Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, compared to variant 1, which results in a longer protein (isoform 2), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK132168.1, SRR1660821.13440.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849387, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGACA TCAAGACGGG CATCTTCGCC AAAAACGTGC AAAAGCGCCT CAACCGCGCG 
CAGGAAAAGG TCCTCCAGAA GCTGGGGAAG GCTGACGAGA CAAAAGATGA ACAGTTTGAA 
GAATATGTCC AGAACTTCAA ACGGCAGGAG GCAGAGGGTA CCAGACTTCA GCGAGAACTC 
CGGGGATATT TAGCAGCAAT CAAAGGTATG CAGGAGGCCT CAATGAAGCT CACTGAATCT 
CTACATGAAG TCTACGAGCC AGACTGGTAT GGACGGGAAG ATGTAAAGAT GGTTGGTGAG 
AAGTGTGATG TGCTGTGGGA AGACTTCCAT CAAAAGCTAG TGGATGGCTC CTTGCTGACA 
CTGGACACCT ACCTGGGCCA GTTTCCTGAC ATAAAGAATC GCATCGCTAA GCGCAGCAGG 
AAGCTTGTGG ATTATGATAG TGCTCGCCAT CACCTGGAGG CTCTGCAGAG TTCCAAACGG 
AAAGATGAGA GTCGGATCTC TAAGGCAGAA GAGGAGTTTC AGAAAGCACA GAAAGTGTTT 
GAAGAGTTCA ATGTTGACTT ACAAGAAGAG TTGCCGTCAT TGTGGTCAAG TCGAGTTGGC 
TTTTACGTCA ATACTTTCAA AAATGTCTCC AGCCTTGAGG CCAAGTTTCA TAAGGAAATC 
GCAGTGCTTT GCCACAAGCT CTATGAAGTG ATGACGAAAC TCGGTGATCA GCATGCTGAC 
AAGGCCTTCA GCATTCAAGG AGCTCCCAGT GACTCGGGTC CTCTTCGCAT TGCAAAGACA 
CCATCACCTC CAGAGGAGCC TTCTCCCCTG CCCAGCCCCA CGGCTAGTCC CAACCACACA 
TTAGCACCTG CATCTCCTGC CCCAGTGCGA CCCAGATCAC CTTCACAGAC AAGGAAAGGG 
CCTCCTGTCC CACCTCTGCC TAAAGTCACC CCAACAAAGG AACTGAAACA GGAGAACATC 
ATCAATTTCT TTGAGGACAA CTTTGTACCA GAAATCAATG TGACAACACC TTCCCAGAAC 
GAAGTCTTGG AGGTGAAGAA GGAAGAGACT TTGCTGGATT TGGACTTTGA CCCTTTCAAG 
CCCGATGTGG CTCCGGCAGG TTCTGCTGCA GCGACCCACT CTCCCATGTC TCAGACATTG 
CCCTGGGACT TATGGACGAC AAGCACTGAT TTGGTACAAC CAGCTTCTGG GGGTTCATTT 
AATGATTTCA CCCAGGCCCA GGACACTTCA TTATTCACCA TGCAGACAGA TCAGAATATG 
GTAGGTGGCT TGGCTGAAAC TGAGCAGGCT CTACCTACAG AGCCCCAAGC CGAAGAGCCT 
CCTGCCACTG CTGCTGCACC TACTGCTGGG CTGGACCTTG GGCTAGAGAT GGAGGAGCCA 
AAGGAGGAGG CTGTGATCCC TCCCGCAACT GATACTGGCG AGACTGTGGA AACTGCAGTG 
CCAACTGAGG GTGCCCCGGT GGAGGAAGCA GAGGCAGAGA AGGCAGCCCT CCCTGCTGGA 
GAAGGAGGAA GTCCAGAGGG AGCCAAAATT GACGGGGAAA GCACGGAGCT TGCAATCAGT 
GAGAGCCCTC AACCAGTGGA GCCCGAAGCG GGTGCTCCTC AGGTCATCCC TTCTGTTGTC 
ATTGAGCCAG CCTCCAACCA TGAAGGAGAA GGAGAACACC AAGAAACTGC TACGGGTACT 
GAACCGAGGG AGGCCGCTGA GGATGTGGCT GCCCAGGGCT CAGCGGGTGA GAAGCAGGAA 
GTGGCTACAG AGCCCACGCC TCTGGATTCT CAGGCAACTC TGCCTGCATC TGCAGGTGCT 
GTGGATGCCT CGCTGTCTGC AGGGGATGCC ACTCAGGAAC TGCCTCCTGG CTTTCTCTAC 
AAGGTGGAAA CCCTGCATGA TTTCGAGGCA GCGAACTCTG ATGAACTTAA CCTACAAAGG 
GGTGACGTGG TACTCGTGGT GCCTTCAGAC TCAGAAGCCG ACCAGGATGC AGGCTGGTTG 
GTGGGGGTGA AGGAGTCAGA CTGGCTTCAG TACAGAGACC TGGCCACGTA CAAAGGCCTC 
TTTCCAGAGA ACTTCACGCG GCGCCTGGAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276475.1
CDS	83..2155
Translation	MADIKTGIFAKNVQKRLNRAQEKVLQKLGKADETKDEQFEEYVQNFKRQEAEGTRLQRELRGYLAAIKGMQEASMKLTESLHEVYEPDWYGREDVKMVGEKCDVLWEDFHQKLVDGSLLTLDTYLGQFPDIKNRIAKRSRKLVDYDSARHHLEALQSSKRKDESRISKAEEEFQKAQKVFEEFNVDLQEELPSLWSSRVGFYVNTFKNVSSLEAKFHKEIAVLCHKLYEVMTKLGDQHADKAFSIQGAPSDSGPLRIAKTPSPPEEPSPLPSPTASPNHTLAPASPAPVRPRSPSQTRKGPPVPPLPKVTPTKELKQENIINFFEDNFVPEINVTTPSQNEVLEVKKEETLLDLDFDPFKPDVAPAGSAAATHSPMSQTLPWDLWTTSTDLVQPASGGSFNDFTQAQDTSLFTMQTDQNMVGGLAETEQALPTEPQAEEPPATAAAPTAGLDLGLEMEEPKEEAVIPPATDTGETVETAVPTEGAPVEEAEAEKAALPAGEGGSPEGAKIDGESTELAISESPQPVEPEAGAPQVIPSVVIEPASNHEGEGEHQETATGTEPREAAEDVAAQGSAGEKQEVATEPTPLDSQATLPASAGAVDASLSAGDATQELPPGFLYKVETLHDFEAANSDELNLQRGDVVLVVPSDSEADQDAGWLVGVKESDWLQYRDLATYKGLFPENFTRRLE

Target ORF information:

RefSeq Version	NM_001289546.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus amphiphysin (Amph), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289546.1

ORF Insert Sequence:

ATGGCCGACA TCAAGACGGG CATCTTCGCC AAAAACGTGC AAAAGCGCCT CAACCGCGCG 
CAGGAAAAGG TCCTCCAGAA GCTGGGGAAG GCTGACGAGA CAAAAGATGA ACAGTTTGAA 
GAATATGTCC AGAACTTCAA ACGGCAGGAG GCAGAGGGTA CCAGACTTCA GCGAGAACTC 
CGGGGATATT TAGCAGCAAT CAAAGGTATG CAGGAGGCCT CAATGAAGCT CACTGAATCT 
CTACATGAAG TCTACGAGCC AGACTGGTAT GGACGGGAAG ATGTAAAGAT GGTTGGTGAG 
AAGTGTGATG TGCTGTGGGA AGACTTCCAT CAAAAGCTAG TGGATGGCTC CTTGCTGACA 
CTGGACACCT ACCTGGGCCA GTTTCCTGAC ATAAAGAATC GCATCGCTAA GCGCAGCAGG 
AAGCTTGTGG ATTATGATAG TGCTCGCCAT CACCTGGAGG CTCTGCAGAG TTCCAAACGG 
AAAGATGAGA GTCGGATCTC TAAGGCAGAA GAGGAGTTTC AGAAAGCACA GAAAGTGTTT 
GAAGAGTTCA ATGTTGACTT ACAAGAAGAG TTGCCGTCAT TGTGGTCAAG TCGAGTTGGC 
TTTTACGTCA ATACTTTCAA AAATGTCTCC AGCCTTGAGG CCAAGTTTCA TAAGGAAATC 
GCAGTGCTTT GCCACAAGCT CTATGAAGTG ATGACGAAAC TCGGTGATCA GCATGCTGAC 
AAGGCCTTCA GCATTCAAGG AGCTCCCAGT GACTCGGGTC CTCTTCGCAT TGCAAAGACA 
CCATCACCTC CAGAGGAGCC TTCTCCCCTG CCCAGCCCCA CGGCTAGTCC CAACCACACA 
TTAGCACCTG CATCTCCTGC CCCAGTGCGA CCCAGATCAC CTTCACAGAC AAGGAAAGGG 
CCTCCTGTCC CACCTCTGCC TAAAGTCACC CCAACAAAGG AACTGAAACA GGAGAACATC 
ATCAATTTCT TTGAGGACAA CTTTGTACCA GAAATCAATG TGACAACACC TTCCCAGAAC 
GAAGTCTTGG AGGTGAAGAA GGAAGAGACT TTGCTGGATT TGGACTTTGA CCCTTTCAAG 
CCCGATGTGG CTCCGGCAGG TTCTGCTGCA GCGACCCACT CTCCCATGTC TCAGACATTG 
CCCTGGGACT TATGGACGAC AAGCACTGAT TTGGTACAAC CAGCTTCTGG GGGTTCATTT 
AATGATTTCA CCCAGGCCCA GGACACTTCA TTATTCACCA TGCAGACAGA TCAGAATATG 
GTAGGTGGCT TGGCTGAAAC TGAGCAGGCT CTACCTACAG AGCCCCAAGC CGAAGAGCCT 
CCTGCCACTG CTGCTGCACC TACTGCTGGG CTGGACCTTG GGCTAGAGAT GGAGGAGCCA 
AAGGAGGAGG CTGTGATCCC TCCCGCAACT GATACTGGCG AGACTGTGGA AACTGCAGTG 
CCAACTGAGG GTGCCCCGGT GGAGGAAGCA GAGGCAGAGA AGGCAGCCCT CCCTGCTGGA 
GAAGGAGGAA GTCCAGAGGG AGCCAAAATT GACGGGGAAA GCACGGAGCT TGCAATCAGT 
GAGAGCCCTC AACCAGTGGA GCCCGAAGCG GGTGCTCCTC AGGTCATCCC TTCTGTTGTC 
ATTGAGCCAG CCTCCAACCA TGAAGGAGAA GGAGAACACC AAGAAACTGC TACGGGTACT 
GAACCGAGGG AGGCCGCTGA GGATGTGGCT GCCCAGGGCT CAGCGGGTGA GAAGCAGGAA 
GTGGCTACAG AGCCCACGCC TCTGGATTCT CAGGCAACTC TGCCTGCATC TGCAGGTGCT 
GTGGATGCCT CGCTGTCTGC AGGGGATGCC ACTCAGGAAC TGCCTCCTGG CTTTCTCTAC 
AAGGTGGAAA CCCTGCATGA TTTCGAGGCA GCGAACTCTG ATGAACTTAA CCTACAAAGG 
GGTGACGTGG TACTCGTGGT GCCTTCAGAC TCAGAAGCCG ACCAGGATGC AGGCTGGTTG 
GTGGGGGTGA AGGAGTCAGA CTGGCTTCAG TACAGAGACC TGGCCACGTA CAAAGGCCTC 
TTTCCAGAGA ACTTCACGCG GCGCCTGGAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Amph ( NM_175007.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_778172.1 Mus musculus amphiphysin (Amph), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu20214
Clone ID Related Accession (Same CDS sequence)	NM_175007.2
Accession Version	NM_175007.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2061bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Mus musculus(house mouse)
Product	amphiphysin isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK132168.1, BC054718.1 and BE949100.1. On Jan 23, 2014 this sequence version replaced NM_175007.1. Transcript Variant: This variant (1) represents the shorter transcript and encodes the shorter isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC054718.1, SRR7345562.1988472.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849387, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGACA TCAAGACGGG CATCTTCGCC AAAAACGTGC AAAAGCGCCT CAACCGCGCG 
CAGGAAAAGG TCCTCCAGAA GCTGGGGAAG GCTGACGAGA CAAAAGATGA ACAGTTTGAA 
GAATATGTCC AGAACTTCAA ACGGCAGGAG GCAGAGGGTA CCAGACTTCA GCGAGAACTC 
CGGGGATATT TAGCAGCAAT CAAAGGTATG CAGGAGGCCT CAATGAAGCT CACTGAATCT 
CTACATGAAG TCTACGAGCC AGACTGGTAT GGACGGGAAG ATGTAAAGAT GGTTGGTGAG 
AAGTGTGATG TGCTGTGGGA AGACTTCCAT CAAAAGCTAG TGGATGGCTC CTTGCTGACA 
CTGGACACCT ACCTGGGCCA GTTTCCTGAC ATAAAGAATC GCATCGCTAA GCGCAGCAGG 
AAGCTTGTGG ATTATGATAG TGCTCGCCAT CACCTGGAGG CTCTGCAGAG TTCCAAACGG 
AAAGATGAGA GTCGGATCTC TAAGGCAGAA GAGGAGTTTC AGAAAGCACA GAAAGTGTTT 
GAAGAGTTCA ATGTTGACTT ACAAGAAGAG TTGCCGTCAT TGTGGTCAAG TCGAGTTGGC 
TTTTACGTCA ATACTTTCAA AAATGTCTCC AGCCTTGAGG CCAAGTTTCA TAAGGAAATC 
GCAGTGCTTT GCCACAAGCT CTATGAAGTG ATGACGAAAC TCGGTGATCA GCATGCTGAC 
AAGGCCTTCA GCATTCAAGG AGCTCCCAGT GACTCGGGTC CTCTTCGCAT TGCAAAGACA 
CCATCACCTC CAGAGGAGCC TTCTCCCCTG CCCAGCCCCA CGGCTAGTCC CAACCACACA 
TTAGCACCTG CATCTCCTGC CCCAGTGCGA CCCAGATCAC CTTCACAGAC AAGGAAAGGG 
CCTCCTGTCC CACCTCTGCC TAAAGTCACC CCAACAAAGG AACTGAAACA GGAGAACATC 
ATCAATTTCT TTGAGGACAA CTTTGTACCA GAAATCAATG TGACAACACC TTCCCAGAAC 
GAAGTCTTGG AGGTGAAGAA GGAAGAGACT TTGCTGGATT TGGACTTTGA CCCTTTCAAG 
CCCGATGTGG CTCCGGCAGG TTCTGCTGCA GCGACCCACT CTCCCATGTC TCAGACATTG 
CCCTGGGACT TATGGACGAC AAGCACTGAT TTGGTACAAC CAGCTTCTGG GGGTTCATTT 
AATGATTTCA CCCAGGCCCA GGACACTTCA TTATTCACCA TGCAGACAGA TCAGAATATG 
GCTGAAACTG AGCAGGCTCT ACCTACAGAG CCCCAAGCCG AAGAGCCTCC TGCCACTGCT 
GCTGCACCTA CTGCTGGGCT GGACCTTGGG CTAGAGATGG AGGAGCCAAA GGAGGAGGCT 
GTGATCCCTC CCGCAACTGA TACTGGCGAG ACTGTGGAAA CTGCAGTGCC AACTGAGGGT 
GCCCCGGTGG AGGAAGCAGA GGCAGAGAAG GCAGCCCTCC CTGCTGGAGA AGGAGGAAGT 
CCAGAGGGAG CCAAAATTGA CGGGGAAAGC ACGGAGCTTG CAATCAGTGA GAGCCCTCAA 
CCAGTGGAGC CCGAAGCGGG TGCTCCTCAG GTCATCCCTT CTGTTGTCAT TGAGCCAGCC 
TCCAACCATG AAGGAGAAGG AGAACACCAA GAAACTGCTA CGGGTACTGA ACCGAGGGAG 
GCCGCTGAGG ATGTGGCTGC CCAGGGCTCA GCGGGTGAGA AGCAGGAAGT GGCTACAGAG 
CCCACGCCTC TGGATTCTCA GGCAACTCTG CCTGCATCTG CAGGTGCTGT GGATGCCTCG 
CTGTCTGCAG GGGATGCCAC TCAGGAACTG CCTCCTGGCT TTCTCTACAA GGTGGAAACC 
CTGCATGATT TCGAGGCAGC GAACTCTGAT GAACTTAACC TACAAAGGGG TGACGTGGTA 
CTCGTGGTGC CTTCAGACTC AGAAGCCGAC CAGGATGCAG GCTGGTTGGT GGGGGTGAAG 
GAGTCAGACT GGCTTCAGTA CAGAGACCTG GCCACGTACA AAGGCCTCTT TCCAGAGAAC 
TTCACGCGGC GCCTGGAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_778172.1
CDS	83..2143
Translation	MADIKTGIFAKNVQKRLNRAQEKVLQKLGKADETKDEQFEEYVQNFKRQEAEGTRLQRELRGYLAAIKGMQEASMKLTESLHEVYEPDWYGREDVKMVGEKCDVLWEDFHQKLVDGSLLTLDTYLGQFPDIKNRIAKRSRKLVDYDSARHHLEALQSSKRKDESRISKAEEEFQKAQKVFEEFNVDLQEELPSLWSSRVGFYVNTFKNVSSLEAKFHKEIAVLCHKLYEVMTKLGDQHADKAFSIQGAPSDSGPLRIAKTPSPPEEPSPLPSPTASPNHTLAPASPAPVRPRSPSQTRKGPPVPPLPKVTPTKELKQENIINFFEDNFVPEINVTTPSQNEVLEVKKEETLLDLDFDPFKPDVAPAGSAAATHSPMSQTLPWDLWTTSTDLVQPASGGSFNDFTQAQDTSLFTMQTDQNMAETEQALPTEPQAEEPPATAAAPTAGLDLGLEMEEPKEEAVIPPATDTGETVETAVPTEGAPVEEAEAEKAALPAGEGGSPEGAKIDGESTELAISESPQPVEPEAGAPQVIPSVVIEPASNHEGEGEHQETATGTEPREAAEDVAAQGSAGEKQEVATEPTPLDSQATLPASAGAVDASLSAGDATQELPPGFLYKVETLHDFEAANSDELNLQRGDVVLVVPSDSEADQDAGWLVGVKESDWLQYRDLATYKGLFPENFTRRLE

Target ORF information:

RefSeq Version	NM_175007.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus amphiphysin (Amph), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175007.2

ORF Insert Sequence:

ATGGCCGACA TCAAGACGGG CATCTTCGCC AAAAACGTGC AAAAGCGCCT CAACCGCGCG 
CAGGAAAAGG TCCTCCAGAA GCTGGGGAAG GCTGACGAGA CAAAAGATGA ACAGTTTGAA 
GAATATGTCC AGAACTTCAA ACGGCAGGAG GCAGAGGGTA CCAGACTTCA GCGAGAACTC 
CGGGGATATT TAGCAGCAAT CAAAGGTATG CAGGAGGCCT CAATGAAGCT CACTGAATCT 
CTACATGAAG TCTACGAGCC AGACTGGTAT GGACGGGAAG ATGTAAAGAT GGTTGGTGAG 
AAGTGTGATG TGCTGTGGGA AGACTTCCAT CAAAAGCTAG TGGATGGCTC CTTGCTGACA 
CTGGACACCT ACCTGGGCCA GTTTCCTGAC ATAAAGAATC GCATCGCTAA GCGCAGCAGG 
AAGCTTGTGG ATTATGATAG TGCTCGCCAT CACCTGGAGG CTCTGCAGAG TTCCAAACGG 
AAAGATGAGA GTCGGATCTC TAAGGCAGAA GAGGAGTTTC AGAAAGCACA GAAAGTGTTT 
GAAGAGTTCA ATGTTGACTT ACAAGAAGAG TTGCCGTCAT TGTGGTCAAG TCGAGTTGGC 
TTTTACGTCA ATACTTTCAA AAATGTCTCC AGCCTTGAGG CCAAGTTTCA TAAGGAAATC 
GCAGTGCTTT GCCACAAGCT CTATGAAGTG ATGACGAAAC TCGGTGATCA GCATGCTGAC 
AAGGCCTTCA GCATTCAAGG AGCTCCCAGT GACTCGGGTC CTCTTCGCAT TGCAAAGACA 
CCATCACCTC CAGAGGAGCC TTCTCCCCTG CCCAGCCCCA CGGCTAGTCC CAACCACACA 
TTAGCACCTG CATCTCCTGC CCCAGTGCGA CCCAGATCAC CTTCACAGAC AAGGAAAGGG 
CCTCCTGTCC CACCTCTGCC TAAAGTCACC CCAACAAAGG AACTGAAACA GGAGAACATC 
ATCAATTTCT TTGAGGACAA CTTTGTACCA GAAATCAATG TGACAACACC TTCCCAGAAC 
GAAGTCTTGG AGGTGAAGAA GGAAGAGACT TTGCTGGATT TGGACTTTGA CCCTTTCAAG 
CCCGATGTGG CTCCGGCAGG TTCTGCTGCA GCGACCCACT CTCCCATGTC TCAGACATTG 
CCCTGGGACT TATGGACGAC AAGCACTGAT TTGGTACAAC CAGCTTCTGG GGGTTCATTT 
AATGATTTCA CCCAGGCCCA GGACACTTCA TTATTCACCA TGCAGACAGA TCAGAATATG 
GCTGAAACTG AGCAGGCTCT ACCTACAGAG CCCCAAGCCG AAGAGCCTCC TGCCACTGCT 
GCTGCACCTA CTGCTGGGCT GGACCTTGGG CTAGAGATGG AGGAGCCAAA GGAGGAGGCT 
GTGATCCCTC CCGCAACTGA TACTGGCGAG ACTGTGGAAA CTGCAGTGCC AACTGAGGGT 
GCCCCGGTGG AGGAAGCAGA GGCAGAGAAG GCAGCCCTCC CTGCTGGAGA AGGAGGAAGT 
CCAGAGGGAG CCAAAATTGA CGGGGAAAGC ACGGAGCTTG CAATCAGTGA GAGCCCTCAA 
CCAGTGGAGC CCGAAGCGGG TGCTCCTCAG GTCATCCCTT CTGTTGTCAT TGAGCCAGCC 
TCCAACCATG AAGGAGAAGG AGAACACCAA GAAACTGCTA CGGGTACTGA ACCGAGGGAG 
GCCGCTGAGG ATGTGGCTGC CCAGGGCTCA GCGGGTGAGA AGCAGGAAGT GGCTACAGAG 
CCCACGCCTC TGGATTCTCA GGCAACTCTG CCTGCATCTG CAGGTGCTGT GGATGCCTCG 
CTGTCTGCAG GGGATGCCAC TCAGGAACTG CCTCCTGGCT TTCTCTACAA GGTGGAAACC 
CTGCATGATT TCGAGGCAGC GAACTCTGAT GAACTTAACC TACAAAGGGG TGACGTGGTA 
CTCGTGGTGC CTTCAGACTC AGAAGCCGAC CAGGATGCAG GCTGGTTGGT GGGGGTGAAG 
GAGTCAGACT GGCTTCAGTA CAGAGACCTG GCCACGTACA AAGGCCTCTT TCCAGAGAAC 
TTCACGCGGC GCCTGGAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Amph ( XM_011244304.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011242606.1 Mus musculus amphiphysin (Amph), transcript variant X1, mRNA.

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CloneID	OMu73047
Clone ID Related Accession (Same CDS sequence)	XM_011244304.3 , XM_011244304.2
Accession Version	XM_011244304.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	amphiphysin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000079.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011244304.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGACA TCAAGACGGG CATCTTCGCC AAAAACGTGC AAAAGCGCCT CAACCGCGCG 
CAGGAAAAGG TCCTCCAGAA GCTGGGGAAG GCTGACGAGA CAAAAGATGA ACAGTTTGAA 
GAATATGTCC AGAACTTCAA ACGGCAGGAG GCAGAGGGTA CCAGACTTCA GCGAGAACTC 
CGGGGATATT TAGCAGCAAT CAAAGGTATG CAGGAGGCCT CAATGAAGCT CACTGAATCT 
CTACATGAAG TCTACGAGCC AGACTGGTAT GGACGGGAAG ATGTAAAGAT GGTTGGTGAG 
AAGTGTGATG TGCTGTGGGA AGACTTCCAT CAAAAGCTAG TGGATGGCTC CTTGCTGACA 
CTGGACACCT ACCTGGGCCA GTTTCCTGAC ATAAAGAATC GCATCGCTAA GCGCAGCAGG 
AAGCTTGTGG ATTATGATAG TGCTCGCCAT CACCTGGAGG CTCTGCAGAG TTCCAAACGG 
AAAGATGAGA GTCGGATCTC TAAGGCAGAA GAGGAGTTTC AGAAAGCACA GAAAGTGTTT 
GAAGAGTTCA ATGTTGACTT ACAAGAAGAG TTGCCGTCAT TGTGGTCAAG TCGAGTTGGC 
TTTTACGTCA ATACTTTCAA AAATGTCTCC AGCCTTGAGG CCAAGTTTCA TAAGGAAATC 
GCAGTGCTTT GCCACAAGCT CTATGAAGTG ATGACGAAAC TCGGTGATCA GCATGCTGAC 
AAGGCCTTCA GCATTCAAGG AGCTCCCAGT GACTCGGGTC CTCTTCGCAT TGCAAAGACA 
CCATCACCTC CAGAGGAGCC TTCTCCCCTG CCCAGCCCCA CGGCTAGTCC CAACCACACA 
TTAGCACCTG CATCTCCTGC CCCAGTGCGA CCCAGATCAC CTTCACAGAC AAGGAAAGGG 
CCTCCTGTCC CACCTCTGCC TAAAGTCACC CCAACAAAGG AACTGAAACA GGAGAACATC 
ATCAATTTCT TTGAGGACAA CTTTGTACCA GAAATCAATG TGACAACACC TTCCCAGAAC 
GAAGTCTTGG AGGTGAAGAA GGAAGAGACT TTGCTGGATT TGGACTTTGA CCCTTTCAAG 
CCCGATGTGG CTCCGGCAGG TTCTGCTGCA GCGACCCACT CTCCCATGTC TCAGACATTG 
CCCTGGGACT TATGGACGAC AAGCACTGAT TTGGTACAAC CAGCTTCTGG GGGTTCATTT 
AATGATTTCA CCCAGGCCCA GGACACTTCA TTATTCACCA TGCAGACAGA TCAGAATATG 
ATCCCTCCCG CAACTGATAC TGGCGAGACT GTGGAAACTG CAGTGCCAAC TGAGGGTGCC 
CCGGTGGAGG AAGCAGAGGC AGAGAAGGCA GCCCTCCCTG CTGGAGAAGG AGGAAGTCCA 
GAGGGAGCCA AAATTGACGG GGAAAGCACG GAGCTTGCAA TCAGTGAGAG CCCTCAACCA 
GTGGAGCCCG AAGCGGGTGC TCCTCAGGTC ATCCCTTCTG TTGTCATTGA GCCAGCCTCC 
AACCATGAAG GAGAAGGAGA ACACCAAGAA ACTGCTACGG GTACTGAACC GAGGGAGGCC 
GCTGAGGATG TGGCTGCCCA GGGCTCAGCG GGTGAGAAGC AGGAAGTGGC TACAGAGCCC 
ACGCCTCTGG ATTCTCAGGC AACTCTGCCT GCATCTGCAG GTGCTGTGGA TGCCTCGCTG 
TCTGCAGGGG ATGCCACTCA GGAACTGCCT CCTGGCTTTC TCTACAAGGT GGAAACCCTG 
CATGATTTCG AGGCAGCGAA CTCTGATGAA CTTAACCTAC AAAGGGGTGA CGTGGTACTC 
GTGGTGCCTT CAGACTCAGA AGCCGACCAG GATGCAGGCT GGTTGGTGGG GGTGAAGGAG 
TCAGACTGGC TTCAGTACAG AGACCTGGCC ACGTACAAAG GCCTCTTTCC AGAGAACTTC 
ACGCGGCGCC TGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011242606.1
CDS	292..2229
Translation	MADIKTGIFAKNVQKRLNRAQEKVLQKLGKADETKDEQFEEYVQNFKRQEAEGTRLQRELRGYLAAIKGMQEASMKLTESLHEVYEPDWYGREDVKMVGEKCDVLWEDFHQKLVDGSLLTLDTYLGQFPDIKNRIAKRSRKLVDYDSARHHLEALQSSKRKDESRISKAEEEFQKAQKVFEEFNVDLQEELPSLWSSRVGFYVNTFKNVSSLEAKFHKEIAVLCHKLYEVMTKLGDQHADKAFSIQGAPSDSGPLRIAKTPSPPEEPSPLPSPTASPNHTLAPASPAPVRPRSPSQTRKGPPVPPLPKVTPTKELKQENIINFFEDNFVPEINVTTPSQNEVLEVKKEETLLDLDFDPFKPDVAPAGSAAATHSPMSQTLPWDLWTTSTDLVQPASGGSFNDFTQAQDTSLFTMQTDQNMIPPATDTGETVETAVPTEGAPVEEAEAEKAALPAGEGGSPEGAKIDGESTELAISESPQPVEPEAGAPQVIPSVVIEPASNHEGEGEHQETATGTEPREAAEDVAAQGSAGEKQEVATEPTPLDSQATLPASAGAVDASLSAGDATQELPPGFLYKVETLHDFEAANSDELNLQRGDVVLVVPSDSEADQDAGWLVGVKESDWLQYRDLATYKGLFPENFTRRLE

Target ORF information:

RefSeq Version	XM_011244304.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus amphiphysin (Amph), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011244304.3

ORF Insert Sequence:

ATGGCCGACA TCAAGACGGG CATCTTCGCC AAAAACGTGC AAAAGCGCCT CAACCGCGCG 
CAGGAAAAGG TCCTCCAGAA GCTGGGGAAG GCTGACGAGA CAAAAGATGA ACAGTTTGAA 
GAATATGTCC AGAACTTCAA ACGGCAGGAG GCAGAGGGTA CCAGACTTCA GCGAGAACTC 
CGGGGATATT TAGCAGCAAT CAAAGGTATG CAGGAGGCCT CAATGAAGCT CACTGAATCT 
CTACATGAAG TCTACGAGCC AGACTGGTAT GGACGGGAAG ATGTAAAGAT GGTTGGTGAG 
AAGTGTGATG TGCTGTGGGA AGACTTCCAT CAAAAGCTAG TGGATGGCTC CTTGCTGACA 
CTGGACACCT ACCTGGGCCA GTTTCCTGAC ATAAAGAATC GCATCGCTAA GCGCAGCAGG 
AAGCTTGTGG ATTATGATAG TGCTCGCCAT CACCTGGAGG CTCTGCAGAG TTCCAAACGG 
AAAGATGAGA GTCGGATCTC TAAGGCAGAA GAGGAGTTTC AGAAAGCACA GAAAGTGTTT 
GAAGAGTTCA ATGTTGACTT ACAAGAAGAG TTGCCGTCAT TGTGGTCAAG TCGAGTTGGC 
TTTTACGTCA ATACTTTCAA AAATGTCTCC AGCCTTGAGG CCAAGTTTCA TAAGGAAATC 
GCAGTGCTTT GCCACAAGCT CTATGAAGTG ATGACGAAAC TCGGTGATCA GCATGCTGAC 
AAGGCCTTCA GCATTCAAGG AGCTCCCAGT GACTCGGGTC CTCTTCGCAT TGCAAAGACA 
CCATCACCTC CAGAGGAGCC TTCTCCCCTG CCCAGCCCCA CGGCTAGTCC CAACCACACA 
TTAGCACCTG CATCTCCTGC CCCAGTGCGA CCCAGATCAC CTTCACAGAC AAGGAAAGGG 
CCTCCTGTCC CACCTCTGCC TAAAGTCACC CCAACAAAGG AACTGAAACA GGAGAACATC 
ATCAATTTCT TTGAGGACAA CTTTGTACCA GAAATCAATG TGACAACACC TTCCCAGAAC 
GAAGTCTTGG AGGTGAAGAA GGAAGAGACT TTGCTGGATT TGGACTTTGA CCCTTTCAAG 
CCCGATGTGG CTCCGGCAGG TTCTGCTGCA GCGACCCACT CTCCCATGTC TCAGACATTG 
CCCTGGGACT TATGGACGAC AAGCACTGAT TTGGTACAAC CAGCTTCTGG GGGTTCATTT 
AATGATTTCA CCCAGGCCCA GGACACTTCA TTATTCACCA TGCAGACAGA TCAGAATATG 
ATCCCTCCCG CAACTGATAC TGGCGAGACT GTGGAAACTG CAGTGCCAAC TGAGGGTGCC 
CCGGTGGAGG AAGCAGAGGC AGAGAAGGCA GCCCTCCCTG CTGGAGAAGG AGGAAGTCCA 
GAGGGAGCCA AAATTGACGG GGAAAGCACG GAGCTTGCAA TCAGTGAGAG CCCTCAACCA 
GTGGAGCCCG AAGCGGGTGC TCCTCAGGTC ATCCCTTCTG TTGTCATTGA GCCAGCCTCC 
AACCATGAAG GAGAAGGAGA ACACCAAGAA ACTGCTACGG GTACTGAACC GAGGGAGGCC 
GCTGAGGATG TGGCTGCCCA GGGCTCAGCG GGTGAGAAGC AGGAAGTGGC TACAGAGCCC 
ACGCCTCTGG ATTCTCAGGC AACTCTGCCT GCATCTGCAG GTGCTGTGGA TGCCTCGCTG 
TCTGCAGGGG ATGCCACTCA GGAACTGCCT CCTGGCTTTC TCTACAAGGT GGAAACCCTG 
CATGATTTCG AGGCAGCGAA CTCTGATGAA CTTAACCTAC AAAGGGGTGA CGTGGTACTC 
GTGGTGCCTT CAGACTCAGA AGCCGACCAG GATGCAGGCT GGTTGGTGGG GGTGAAGGAG 
TCAGACTGGC TTCAGTACAG AGACCTGGCC ACGTACAAAG GCCTCTTTCC AGAGAACTTC 
ACGCGGCGCC TGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

EHBP1L1 coordinates Rab8 and Bin1 to regulate apical-directed transport in polarized epithelial cells.
The Journal of cell biology212(3)297-306(2016 Feb)
Nakajo A,Yoshimura S,Togawa H,Kunii M,Iwano T,Izumi A,Noguchi Y,Watanabe A,Goto A,Sato T,Harada A

Exocytosis regulates trafficking of GABA and glycine heterotransporters in spinal cord glutamatergic synapses: a mechanism for the excessive heterotransporter-induced release of glutamate in experimental amyotrophic lateral sclerosis.
Neurobiology of disease74314-24(2015 Feb)
Milanese M,Bonifacino T,Fedele E,Rebosio C,Cattaneo L,Benfenati F,Usai C,Bonanno G

Stromally expressed ?-catenin modulates Wnt9b signaling in the ureteric epithelium.
PloS one10(3)e0120347(2015)
Boivin FJ,Sarin S,Lim J,Javidan A,Svajger B,Khalili H,Bridgewater D

Mdm2 is required for maintenance of the nephrogenic niche.
Developmental biology387(1)1-14(2014 Mar)
Hilliard SA,Yao X,El-Dahr SS

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Amph cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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